LOCUS       DAONFI010000001        30070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_1507_length_30070_cov_4.751424, whole genome shotgun sequence.
ACCESSION   DAONFI010000001 DAONFI010000000
VERSION     DAONFI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 30070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30070
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1507_length_30070_cov_4.751424"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLK08_00005"
     CDS             complement(<1..168)
                     /locus_tag="PLK08_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPS51914.1"
                     /translation="MVNKATEDKLVLGIPKGSLQDSTLDLFRRAGYNITISSRSYFPT
                     IDDPEIECMLIR"
     gene            complement(185..1210)
                     /locus_tag="PLK08_00010"
     CDS             complement(185..1210)
                     /locus_tag="PLK08_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51915.1"
                     /translation="MNNAKQYEPKMLKLLKSIKKSASIEMVEPAAALPVMVRAILQAD
                     AADRQFAKAIAALEKEYVDLNELRVSQTKEIVECIGEDYPDARGKAVEIVRSLNNLFD
                     KVGKLSLEPLVEMNKRDIRKLLKDIGLGNYAEGYLSMAVFGIHAIPVDELLAGLLEVQ
                     ELVAPGSDLHDIQAFLERIIKQKDGIASYVFLHAYVDENRKLANKGYSDRYERLLAKR
                     KDEAERLAREAEERKKVEENDIENGNDFEMEEPIDEDVAVVDEEIDEEVDIEIEIEPE
                     DESADKEKQETATEEKKSEPAPAKAAKKPDSKKVKTADKAKSKPLVKKKAAKPAKPAK
                     SKKDSKK"
     gene            complement(1214..1621)
                     /gene="rbfA"
                     /locus_tag="PLK08_00015"
     CDS             complement(1214..1621)
                     /gene="rbfA"
                     /locus_tag="PLK08_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPS51916.1"
                     /translation="MTRRTDRIGNLIRNTLGDILLSKMSDPRFDPAKVSVTRVEVPED
                     LLTARVYVSVLGEKKQQSLALAALNSARGFLQEKLAHEISLRNTPRLSFVIDEQFKKT
                     METLNILQEVAQELREKDEARAQQQQESASEDD"
     gene            complement(1662..4382)
                     /gene="infB"
                     /locus_tag="PLK08_00020"
     CDS             complement(1662..4382)
                     /gene="infB"
                     /locus_tag="PLK08_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPS51917.1"
                     /translation="MAKKRIHELAKELNVTSKAIIDKCAAEGLDDIVKTASSGISAGL
                     EETIRDWFSEGISDVESTAVETSDRVDIEKAHEEAQKTRRRGKKEDEESQAVAEEQTG
                     EEIPAAAEQPTEQIETIAEVKEEVPPQPTTAVVTAQEQPAEQPTTAPEPIQQESPKQQ
                     PAATDEKPAAAERPQKRGKTVKPAGPMLIPKPAQLKGPRVVRMEQPDYAPKPQGRFTP
                     ARSTYGAPHAPAMDLSLERKIAENDEQKRKTNKRRSPRRKGKTNAEVTTEQLREWRNV
                     DLIERAERIADAGHTLRRHRPSSGTKRPGQSGQSTTAATKSNKVEIQEPITIKDISAA
                     TGIKASDMIKKLMQSGMLVTINQVLSTEKAEELMIEYGVELVIAKAKSEEEELIEQLE
                     SRPKTKLTSRSPVVTFLGHVDHGKTSLLDYLRKSSVAAGEAGGITQAIGAYRHEMEGG
                     RYVVFLDTPGHEAFTAMRARGANMTDVVVLVVAADDGVMPQTIEAISHAKAAGVPIVV
                     ALNKIDVPNANPQRAMSQLADQGLNPREWGGDVEVIQTSAIKGTGMADLLEVLLLEAE
                     LLELKAEKEAPASGYVVEARMDPGRGVVATLLNINGTLKVGDTILCGKGYGRVRQIYD
                     DKGKTLDFAGPANPVAITGLDEVPEAGDKFYVVDDLEKAREVAEQRRTETRNKELAAT
                     AKTPRSLEELLGKINEGEIVELPIILKADVQGSIEAIVGTLKKLGSSETRLNIIHAGV
                     GGISSGDVMLAEASNAVIIGFNVVADTVARKLAEQKGVDIRTYRVIYDIVEDMTLALE
                     KGLAPEMREEELGQAEIRQLFRISRVGTIAGCYVTEGLVSRNAKVRIIRDSVVIEDGR
                     DVDSLKRLKDDAREVKAGLECGIKIKGYDDLKESDILAFYRNVEVARKLG"
     gene            complement(4659..5849)
                     /gene="nusA"
                     /locus_tag="PLK08_00025"
     CDS             complement(4659..5849)
                     /gene="nusA"
                     /locus_tag="PLK08_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPS51918.1"
                     /translation="MNQEMLRIVNGIAREKNIDPGVVFDDLEMAMRSAIRKANTPETE
                     IEVSINRATGDIIAVIDGEAMSIAALGRIAAQTAKQVLIQRIREAERDSIFDEYAQQK
                     GKIVTGRVARWEGGSLVVDLDRGEAILPKSEQIPGDSHHIDERIRAMILDVREQPGNV
                     KIILSRTHPDFIRALFELEVPEVSEGIIEINALAREAGYRTKVAVNSLDSKVDAVGAC
                     VGIRGSRIKNIVDELGGEKIDIVRWNESSKNLIGNALKPAEVTEIALCFELGRATVVV
                     TDDQLSLAIGKRGQNVRLAARLTNWDIDILTPDEYNTALDLLEKTAKSIEGVDPAIVD
                     KIIAMGMVSVLDVDEVGPDPLVEELGIEQPVAQAIVEACTEASKNVDETKTKAAQVQR
                     QELP"
     gene            6496..6954
                     /locus_tag="PLK08_00030"
     CDS             6496..6954
                     /locus_tag="PLK08_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPS51919.1"
                     /translation="MIFDSTDNLDLYFDAIPELEAVAEFLAAMPADAAGRTSIEGDDL
                     YVNINEYETRLRGEGRYEFHQKYIDVQVVLEGREELDVLNETNLELTEAYDETVDVGF
                     YARGSQAAGATLRMIPGRFAVLFPQDIHQPCIAANDEPSKVKKAVFKILF"
     gene            complement(7043..7126)
                     /locus_tag="PLK08_00035"
     tRNA            complement(7043..7126)
                     /locus_tag="PLK08_00035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7090..7092),aa:Leu,seq:caa)
     gene            complement(7227..8387)
                     /locus_tag="PLK08_00040"
     CDS             complement(7227..8387)
                     /locus_tag="PLK08_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPS51920.1"
                     /translation="MIKREYFDPEELAIGLSHYDLGIIKSIREFPRGSSRAPKCIVEC
                     ERGRYLFKRRQHGVENLDKVAFTHHIQLSLAAQNFPLPHLQPTRDDGNSMLIFNDHIY
                     ELQEFIAGTGYDQSLKATEQSGHALGLYHKLLEHFRSEYEPPTASYHNAKAIHHSILH
                     CASAMKSKCDQASNTIDNTVTILDEYYRKCVEKLNKIGLHHWPSQIVHGDWHPGNMLF
                     RDQLVAAVIDYDTARLHQRVIDFANGALQFSIIGGGDNPLEWPANLDMPRFKAFLHGY
                     DSVNVITQDELKSIPLLMCEAIISEAVLPIASTGGFGRFNGCEFLQMIRKKVEWILKN
                     YSALVNILNTPIPEYAGLPPQNASSQTARKPIEDIDDPLSGLAEIVERINPK"
     gene            8673..9437
                     /locus_tag="PLK08_00045"
     CDS             8673..9437
                     /locus_tag="PLK08_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPS51921.1"
                     /translation="MIDCHLHLQLAPLNAQICRHVQSAGMKGVDSFWCNATSCDDFQA
                     VAEIALQYGQIVPFFGIHPFFVADSPADWLERLESYLEKFPTAGLGEAGLDCWFKKCK
                     NTLELQKKFLAEQIRLADRLSRPMALHSLNADGAMLDLLEQNTPQRPFMMHAFRGSPE
                     TVGRLASMGAYFSFAGNILNPQARKARQAVVAVPADRLLIETDSPELKIFGYDYPTLP
                     EYLGLVRDEVAKILKTTPQDVENLTLENAKKFLMGQ"
     gene            9476..10261
                     /locus_tag="PLK08_00050"
     CDS             9476..10261
                     /locus_tag="PLK08_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPS51922.1"
                     /translation="MDRFLRTRMLLGEKAMERLDDSHVVIVGLGAVGGYAMEGIARGG
                     VGNLRLVDFDVVSPTNINRQLLALDSTVGQPKVELAAARVKDINPACHVQAMRAFLHT
                     DTLDEILAGRVDMVIDAIDALLPKVELAAACIERKIPIISSMGAALRKDPSFIKIGPL
                     KKATHCPLARRVRKMLRKRGLTTDITCVFSSEPTCELEESAMAELENSEQGVILRGRP
                     RRSLGSLPTITGIFGLTIANAVIMALLQNPSEDETTAITPNNA"
     gene            10376..11704
                     /locus_tag="PLK08_00055"
     CDS             10376..11704
                     /locus_tag="PLK08_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPS51923.1"
                     /translation="MKNDYKIGLVIGLLVLFLGVGYFIFRGPGKTRKPEIPTVPETPA
                     AENTFVGETTATGSSTTSEFPPPSMSGETTPANNTVAVATAAPAAETVSPAAAKPAQS
                     STLAERVDNWSTRTGNENAAENSPGASETLSPRLADNNAGGTASSETVAVKPAAGSLK
                     TPPAEAAVEWGVPAVESTTSASQNNATTVSSVEPVGGTTPSTYVVKPNDVGWYIAENV
                     YGKGMGQYWKLIRQANPDVDMSNLKAGQVLKIPPLPENARQRLRDTVTQGTTFTDATG
                     KNIYVTKSGDSFWTIAQRVYGKGYRYKEIQKANPGVETLRPGLRLVIPGEVVSTSSVA
                     GEMTTYGSDTVTTETSAANYYTVKSGDSLWTIAEKEFGDGSYADLIKKANPNINANNL
                     RVGQRIVLPAKPEGTPATSTHTSDENTAERRHSAPSSSEPVPSDEPDFGL"
     gene            complement(11699..12040)
                     /locus_tag="PLK08_00060"
     CDS             complement(11699..12040)
                     /locus_tag="PLK08_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51924.1"
                     /translation="MQARFVILFHSGNGQDHYDLMIETGERLATWQFLSFPAAGAICR
                     QLPPHRKAYLTYQGHVSNRRGEVTRIDKGFCEPAETADGLRVSLRGKKIAGEFLLSKI
                     AGDEWSFNIAS"
     gene            complement(12196..12990)
                     /locus_tag="PLK08_00065"
     CDS             complement(12196..12990)
                     /locus_tag="PLK08_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51925.1"
                     /translation="MAVSKKTVRIITGVVLALMLLLAILFLMASTVPGDYGPFQLTQQ
                     ERKEAAQDFINKHGAPLLVQIRRPEPFEHTITEDELNYYFASLEEIAFLKPAKRNQER
                     KTSGVYEAMDAAGFADPAVRLDDGVMTLMVRSRAANKVISVDIGFDMTDDRHLLISIK
                     SVRIGKLAVPQTLVAGSINTFKTAVGPQDAITEESLRDLDSIIANVIAKINDKPFPTE
                     FKLGSKRLREIRDFKIDDGKLTVKIVPVQKDDKTADSPESKSSDKD"
     gene            13481..13753
                     /gene="rpsO"
                     /locus_tag="PLK08_00070"
     CDS             13481..13753
                     /gene="rpsO"
                     /locus_tag="PLK08_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPS51926.1"
                     /translation="MSTVAKEVKESIVSENRIHEGDTGSAGVQIALLTARINHLAEHL
                     KSHVKDHSSRRGLLKMVGKRNSLLKYLSARQPERYHELIAKLGLRK"
     gene            13956..16190
                     /locus_tag="PLK08_00075"
     CDS             13956..16190
                     /locus_tag="PLK08_00075"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPS51927.1"
                     /translation="MSSLSKVVKVQKEIGGRVLSFEMGKWAKQANSAVVVTYGETVVL
                     ATVLVGPAGSDIDFFPLYVDYREAQYAAGKIPGGFFKREGRPSTKEILTMRMIDRPIR
                     PLFPYDYRCEVQIQCMVLSSDNENDPDLIAMIGASAALAASSAPFQGPIGACRVGYID
                     GQYVVNPTFSQLAQSEMELLVAGPKEAINMIEFTGREMPEDVVAGATDLGHKIDCDII
                     ALTEEFVSQLGVCKEYEPTVMPEELKKIAAERCSAAIREKKLIPGKQERNEALDEVRQ
                     ALIDELIPEGAAPETLPYTEAQIKEVFYKLEGRIQRELILEGKRPDGRGYEDIRPLYT
                     EVNVLPRTHGSAIFQRGETQALVTATLGTPRDQPIIDGLQDEYKKPFFMHYNFPPFSV
                     GEIRPLRGPGRREIGHGALAEKSLEPIMPSNEVFPYTVRLVADILESNGSTSQAATCG
                     GTLALLAAGVPIKAPVAGISIGMVSDDEAGKYVLLTDIVGEEDFHGDMDFKVAGTNKG
                     ITGIQLDMKARGIKQDRIVATFAQARRARLRILEAMAATISEPAPMSDYAPRMLTVKI
                     DPEKIGKVIGPGGKMINKIQDETGATIDIEEDGTIFIASSGEGAEQAKAMIQAITENP
                     EIGKIYKGRVVGIREFGAFVEILPGQEGLCHVSEMSDEYVADPNDFCKLGDEMEVRII
                     GIDDTGKIKVSRKAALNPDWTIDDVKPAPSRRGSDRGSRNGGERRGGGSHRNGGDRNR
                     RDRE"
     gene            complement(16297..17187)
                     /gene="accD"
                     /locus_tag="PLK08_00080"
     CDS             complement(16297..17187)
                     /gene="accD"
                     /locus_tag="PLK08_00080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPS51928.1"
                     /translation="MAIQNDSLSAEQEKNIAFDASTLSGPPQRVKKDLPDGLWLKCPA
                     CESTLYRKNVTENQEVCPECQYHFRMTGRQRIAQLADEDSFEEFFTDIKPADPLKFEW
                     SGKQYAERLAAEQKKTGLDDSILTGVSFIKGRRVALGVMAGDFLMGSMGSVLGEKLSL
                     LIEFATKENLPLVVVCISGGARMHEGALSLMQMAKTSAALARYDQAGGLYISVLTDPT
                     TGGTTASFAMLGDVIIAEPKALIGFAGPRVIANTIKAELPENFQTAEFLLEKGFVDMI
                     VHRHQLRSEIARIIDYCKPS"
     gene            complement(17226..18068)
                     /gene="rpe"
                     /locus_tag="PLK08_00085"
     CDS             complement(17226..18068)
                     /gene="rpe"
                     /locus_tag="PLK08_00085"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPS51929.1"
                     /translation="MCNKSADVSRQPEHAVNAAHRRLRNDIETNSQPVGRINVLELQM
                     GQNMWTSLPAGPAIAPSLLSVDFARAGEQIDQVLAAGAEMLHMDIMDGHFVPNLSMGV
                     PVVEKFRRYTDAPLDVHLMVSDPGFFFEPFAKAGADSLNFHIEACGRWGTGMADEAKR
                     LIERIRKMNLGVGVTIKPQSPVCLLQPIIDLVDMILVMTVEPGFGGQSFMADQMPKVS
                     EIKNMLKPHQILQVDGGIDVSTITVAHKAGADVFVAGNSVFATQDPGAAVKALKARLI
                     ENSL"
     gene            18202..18744
                     /locus_tag="PLK08_00090"
     CDS             18202..18744
                     /locus_tag="PLK08_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPS51930.1"
                     /translation="MRRDEILQMDDENLIALCDMHIYKASGPGGQHRNKVSSAVRLRH
                     RETGISATANDSRSQHENKVLAVNRLRMNMALRLRENVDIVKLVLPTYLVGCIFKPKK
                     PREGAAGMRLEIGRKDRRFWPTAAWLLDLLVACGGKIGDAARLLNISTGNLTSIFQSD
                     RHLWAAVQEIRRDAGLNHLT"
     gene            18749..19366
                     /locus_tag="PLK08_00095"
     CDS             18749..19366
                     /locus_tag="PLK08_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51931.1"
                     /translation="MSRKDRHIELTEKGFSLIDGGQAAAEFDWIHVTEIVAYRRDPEG
                     EDLLCLGFRAAASRNYVEVDEETDGYSKLLAAMYEAFPAISRDWWQDIAQPLGTNRET
                     IYGMAMSEENEEPAASKYLKSLSLQKKQSRLRRRLLLVMLSAAVVFGIILGLLSARSS
                     GGWLVIVGCAIQFIVSTAVLRRIKSAALTLVLFAVSYWLAAFLCR"
     gene            complement(19736..19921)
                     /locus_tag="PLK08_00100"
     CDS             complement(19736..19921)
                     /locus_tag="PLK08_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51932.1"
                     /translation="MELLLLPDFADLETFFDACFLAGAGAAFLTLFLTAFFAAFFIAA
                     FGAVFLTAFDEDFFATV"
     gene            19974..20465
                     /locus_tag="PLK08_00105"
     CDS             19974..20465
                     /locus_tag="PLK08_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPS51933.1"
                     /translation="MAVNNKLSKKATNKDKVKSVLSKKELEEFRRILIEKRKGILGDM
                     AGMRGSSMGMNLQDSAGDLSNMPTHLADIGSDNFEHEFTLELLESERDLLREIDEALD
                     RIDDGTFGICLGTGQPISIARLKARPWCRYSIEYKRMIEKGLAAPRHSSDDEEHHERD
                     DEE"
     gene            20533..21219
                     /locus_tag="PLK08_00110"
     CDS             20533..21219
                     /locus_tag="PLK08_00110"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPS51934.1"
                     /translation="MEDRKKNQPAYKSARAWTIFLSIAVLAAAADLWSKHAVFSWLLN
                     DNSQQLSNKAANDAAYLKECHSAELPPADSEAFSRVVLQRLHIHRDVCRGLRFTLSTN
                     PGVVFGYRGMPRYVVNIFTVAMMLAVCMFFSFCSRRDYWLITAFALILGGAAGNLYDR
                     LFSVVSISPLVPIRFHVRDFIDCSDIGYQWVFNVADAWLVIGVAMVLLNSLWLWRKEM
                     RNKNAGVAAK"
     gene            21216..21635
                     /locus_tag="PLK08_00115"
     CDS             21216..21635
                     /locus_tag="PLK08_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51935.1"
                     /translation="MKIFLAGIMQGSVRDDTIHPQDWRGPIREIILRRIPDAEVYCHY
                     SEHPNSLTYSGGKIRETFDDGVRRCAGSDLVIAYLPSASMGTAIEMYEAYQNGAVVLT
                     VTPMAANWVVRLYSHAVFATLEELDVFLADGRLEAFF"
     gene            21637..22656
                     /gene="rlmN"
                     /locus_tag="PLK08_00120"
     CDS             21637..22656
                     /gene="rlmN"
                     /locus_tag="PLK08_00120"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPS51936.1"
                     /translation="MIHILDITPERFCEHIERIGEKKFRATQILQWIWQKRVTDFALM
                     SDLSKKLRENLFGEFDIITGSIAARRDSGDGCVKLLVEYPDGERIETVAIPAEERLTA
                     CVSTQAGCAMACSFCASGLDGLRRNLTSGEIVQQVLLLEQATGQRVTNVVFMGMGEPL
                     ANLDATINAVRTLADSRRGAISARKITVSTSGLPKQIRQLAELELPITLAISLHAPND
                     ELRRKLMPVASRYPIAEIISAAAEFFNSHKREVTLEYTLIGGINDSPQCAAQLAAIAA
                     TLRCNVNLIRYNPVSSLPYVRPDERVVNDFAELLRRKHVNVTVRRSKGIDADSACGQL
                     RRRNA"
     gene            22915..23502
                     /locus_tag="PLK08_00125"
     CDS             22915..23502
                     /locus_tag="PLK08_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPS51937.1"
                     /translation="MTDQTKSTDRNAELMLQFKAGNHGAFEELVRRNLGNVHSLIFRF
                     MRNESHVDDLTQETFLRIYRHAESYQPTAKFSTWLYRIVANLCFNAIRAKKYQRTYSL
                     DNSNDNDVSVDLQDKAQRTPSEKLNDDELNRIIKNAIDDLPENQRLAIILKQYEQLNY
                     DEIANVMEISQSAVKSLLSRARMTLRTKLSRYLTA"
     gene            23663..24295
                     /locus_tag="PLK08_00130"
     CDS             23663..24295
                     /locus_tag="PLK08_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51938.1"
                     /translation="MNTNDLDNISAWMDGELDAEESEKVRKILFDDAQAAMTCADFRK
                     LDKFMKRWETPKPAEFLGDVICLAAEHDPAKSPFYRITRLAVPLTMAAIAVLAIMMWV
                     GGARSTDDAASLAWSHLTPAQQSQASRSAKAFLLLPQQEQAKILAEFQKRFNISPSQN
                     DWVGPVLSSFTLQERDELLKLSPVRRLEILRKRQLEMAASGTISQKIPQE"
     gene            24606..25067
                     /locus_tag="PLK08_00135"
     CDS             24606..25067
                     /locus_tag="PLK08_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51939.1"
                     /translation="MEEDKNINSLEDIWPLQAALNSRAGFDTLALGKSLNTDETNMEI
                     RLHVGRALKNYIDAMASECNELQDCLSWKHWYAEAKAGRQYQLQDLQNARVEATDMLF
                     FWISICQLLGLESEDVYRLYEKKLGINHRRQDENRTQAEHGSHEDENRKVL"
     gene            complement(25211..26767)
                     /locus_tag="PLK08_00140"
     CDS             complement(25211..26767)
                     /locus_tag="PLK08_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPS51940.1"
                     /translation="MTTDISDATYEKNLRELEALYGIANVLSASRQQQQMLEEALDVL
                     STQLDMHRGTVMLLSADGEELIAEIGTDFVANKGRQIRYRRGEGVTGQVVATGKSAIV
                     PKISQEPHFLDRIHQRRANLTQELSFICVPIKIDNETVGTLSVDKLYNPSENLEMDER
                     VLSIVASMIAEDVKSRRESQALRQSLEEENLRLRSELADKWRPEKIVGNSGLMREVYQ
                     QIHQVAQADTTVVIRGESGTGKELVAHAIHYSSLRAEGSFVKVNCAALNENLLESELF
                     GHEKGAFTGAIQTRKGRIEEAESGTLFLDEIGDFPPSTQIKLLRVLQEREYERVGSNQ
                     TRTANVRIICATNRNLEEAIEDGSFRQDLYYRINVFPIFLPPLRDRKDDILLLADFFV
                     EKYAKQFNKPVGRISTPAINMMVQYHWPGNVRELENCIERAVLVSTDGVIHGHHLPPS
                     LQTPEITGTFGTGSLDEKVAIFERDIIVDSLKRTRGNMAASARELGTTPRILRYKVKN
                     LNVDPEKFKK"
     gene            27266..28558
                     /locus_tag="PLK08_00145"
     CDS             27266..28558
                     /locus_tag="PLK08_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPS51941.1"
                     /translation="MLSAAPCFAGDAPAATADSVQLNLNIVWTCVAAFLVFFMQAGFA
                     CVETGFTRAKNAVNIMMKNLMDFVAATLVFFLVGFGLMFGKSNGFFGTTLFGMSGVEP
                     GDSWDWTFLMFQTVFAGTSATIVSGAMAERTKFVGYLIYSAFISLLIYPIFGSWAWGN
                     LLITDNCSWLANMGFHDFAGSTVVHSIGGWLALAGAVVLGPRIGKYRPDGKPAAIIGH
                     NLPLAALGVFILWFGWFGFNPGSTTVGSGLIGYIAITTNLAAAGGALAAIAVAWGIFS
                     KPDASMALNGALAGLVAITAGCDTVSPLGAIIIGLCAGVLVVLAVTFIDRILKIDDPV
                     GAVSVHGVCGAFGTLAVGLFSMSDGLLYTGSLKLLGTQALGVATAFAWAFVLGMILFT
                     VINKTIGLRVSREEELRGLDIGEHGMEAYAGFQIFSNI"
     gene            28595..28936
                     /locus_tag="PLK08_00150"
     CDS             28595..28936
                     /locus_tag="PLK08_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPS51942.1"
                     /translation="MKLIIAYIQPERLNDVKQALYADEIHKMSVSNVMGCGQQMGYHE
                     TYRGVRLEVNLLKKVRLEIAVNDKFVKPTIDAIIKGARTGHIGDGKIFIVTLDECIRI
                     RTGETGGEAIG"
     gene            complement(29248..>30070)
                     /locus_tag="PLK08_00155"
     CDS             complement(29248..>30070)
                     /locus_tag="PLK08_00155"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51943.1"
                     /translation="SSAAVFCASYNAAVCGCEKCCTFHNADVTLLKNFSAGSVVHLDP
                     DRRVTGRRSASLADYSPGPRFVEELIRQPGLRGVIKLSPAMPKDELAALCMPLDIEYV
                     STHNTCRQLLAWFPAVGEGSRLATVIRGEYPDYRVDSVFAAAGPAAYGAAAGKYLIEP
                     DAAVLAAGAESALAMKLSAWKLAPGLVWLFADKKPATDTAGQIFEIIQSVPGRLDDIK
                     KALKNLNAGNVELKTRGVDLNADKVQRQLHGKGTQPLTVLWGRVGLNQLAFIAKR"
BASE COUNT         7138 a         7544 c         7836 g         7552 t
ORIGIN      
        1 tcgaatcagc atacactcga tctccggatc atcaatcgtc ggaaaataac tgcgtgacga
       61 aatcgtaatg ttgtagccag ctcgccggaa aagatccagc gtactgtcct gcaatgaacc
      121 ttttggaatg cccagaacca acttgtcttc tgtcgccttg ttcaccatca cttgattctc
      181 tttcttattt ttttgagtct tttttcgatt ttgcaggttt tgccggcttg gccgccttct
      241 tttttaccaa aggcttggat ttggccttgt cagccgtctt tacttttttg gagtctggtt
      301 ttttggcggc cttggctggc gccggctcag attttttttc ttctgtagca gtttcctgct
      361 tttccttatc agccgattcg tcttccggtt ctatttctat ttcgatgtcg acttcttcgt
      421 caatctcttc gtcaactact gcgacgtctt catcgatagg ttcttccatt tcaaaatcat
      481 tgccgttttc gatgtcgttt tcttccacct ttttacgttc ttctgcttca cgggcgagac
      541 gctcggcttc gtccttgcgt tttgcgagca gtctttcata acggtcacta tagcccttat
      601 ttgctagttt acgattttca tcgacatatg cgtggaggaa gacgtatgac gctatgccgt
      661 ctttctgttt gattatccgt tcgagaaatg cctggatatc gtgaaggtcc gaccccggag
      721 ccactaattc ctggacttcc agcaggccag ccagcaattc atccaccgga atggcgtgta
      781 tgccgaaaac cgccatcgac aagtagcctt cggcgtagtt gcccaagccg atatccttga
      841 gcagtttgcg gatatcacgt ttgttcatct cgacaagcgg ctcaagcgaa agtttgccga
      901 ccttgtcaaa gaggttgttt aacgagcgga ctatttcgac ggctttgccg cgggcgtcag
      961 gataatcctc gccgatgcac tcgacgattt ccttggtctg ggaaactcgc agttcattaa
     1021 ggtcgacata ctccttttcc agcgcggcta tcgctttggc aaactgtcgg tcggcggcgt
     1081 cggcttgaag tatggctcgg accatgaccg gcagtgcggc agccggctct accatctcta
     1141 ttgaagccga tttttttatc gacttcagca gttttaacat tttcggttca tactgtttgg
     1201 cattattcat cgttcagtca tcctcactgg cggattcctg ttgttgttga gcccgcgctt
     1261 catctttttc acgaagctcc tgggctactt cctggagaat attcagggtt tccatggttt
     1321 ttttgaattg ttcatctatt acgaaagaca gcctgggggt attgcggagg cttatctcgt
     1381 gagccagctt ttcctgcaaa aagcccctcg cactgttaag cgccgcgaga gccagactct
     1441 gctgtttctt ttctcccaga acagacacgt aaacccgagc cgtaagaaga tcttccggca
     1501 cttcaacgcg ggtaacagat acctttgcag ggtcaaaccg cgggtcgctc attttactga
     1561 gcagtatgtc gcccaaagtg ttacgtatca aattgcctat tctgtcagtt cgtctggtca
     1621 tggcagtatt ttgaataaaa agcttttttg ttttttaaaa atcagccgag tttacgggcc
     1681 acttccacat tgcggtagaa cgcgagtata tcgctttcct tgaggtcgtc gtagcctttg
     1741 attttaatgc cgcattccag gccagccttg acttcacggg cgtcgtcttt gagccgtttg
     1801 aggctgtcga cgtctctgcc gtcttcaata acgacactgt cgcggatgat gcggactttg
     1861 gcattgcggc tgacgaggcc ttccgtcaca tagcagccgg caatggtgcc cacgcgtgaa
     1921 atacggaaca actgccgtat ttcggcttgt ccgagttctt cttcgcgcat ttcaggcgcc
     1981 aggccttttt caagtgccag cgtcatgtct tccacgatat cgtatatgac gcggtacgtt
     2041 ctgatgtcca cgcctttctg ttcggcgagt tttctggcga cggtgtcggc gacgacgttg
     2101 aagccgataa ttacggcgtt cgacgcctcc gccagcatta cgtcgccgga agaaattccg
     2161 ccgacgccgg cgtggatgat gttcagtcgc gtttcactgc tgccgagttt tttcaatgtg
     2221 ccgacgatag cttctataga accctgaacg tcggctttga ggattatggg cagttccaca
     2281 atttcgcctt cgtttatttt gccgagcagt tcttcgaggc ttcgcggtgt cttagccgta
     2341 gcggcaagtt ccttgttgcg ggtttctgtg cgtcgttgtt ccgccacttc tcgcgccttt
     2401 tcgagatcgt cgacgacgta gaatttatcg ccggcttccg ggacttcgtc caggccggtg
     2461 atggccaccg ggtttgcagg gccggcgaaa tccagcgtct tgcccttgtc gtcataaatc
     2521 tgtcgtaccc ggccataacc tttgccgcac aatatggtat cgcccacttt cagagtgccg
     2581 ttgatattca gaagcgtcgc cactacgccg cggccgggat ccatgcgggc ttccacgaca
     2641 tagccgctgg cgggtgcctc tttttcagcc ttgagttcaa gcagttccgc ctccagcagc
     2701 agaacctcca gcaggtcagc catgcccgtg cccttgattg ccgaagtctg aatgacttcg
     2761 acatcgccgc cccattcacg cggattgagg ccctggtcag ccaactgtga catcgcccgc
     2821 tgcggattgg cgttggggac gtcgatttta ttcaacgcga cgacaatcgg gacgccggcg
     2881 gcttttgcgt ggctgatggc ttcgatggtt tgcggcatga cgccgtcgtc agccgccacg
     2941 accagcacta ccacgtccgt catgttcgca ccgcgggcgc gcatggcggt aaacgcttca
     3001 tggcccggcg tatccaggaa gacgacgtaa cggccgcctt ccatttcatg ccgatatgcg
     3061 ccgatagcct gcgtgatacc gccggcttcg ccggcggcta cggacgattt ccgcagataa
     3121 tccagtagtg acgttttgcc gtggtctacg tggccgagga acgtaacaac cggagaacgc
     3181 gacgtaagtt tcgttttggg ccggctttcg agctgttcga tgagctcttc ttcctcgctc
     3241 ttggccttgg cgattacaag ttccacgccg tattctatca taagttcttc cgctttttcg
     3301 gtgctgagaa cctgatttat ggtcaccagc atgccggatt gcataagttt ttttatcata
     3361 tccgaggctt ttatgccggt agcggcggaa atgtctttaa ttgttatagg ttcctgaatt
     3421 tcaactttgt tggattttgt ggcggcagtc gtcgactggc ccgactggcc aggccgcttc
     3481 gttcctgagg atggccgatg tcttcgcagc gtatggccgg cgtcggcgat gcgctccgca
     3541 cgctctatca ggtcgacatt gcgccattca cgaagctgtt ccgtagtcac ttcggcattg
     3601 gttttgcctt ttcgacgcgg gctgcgacgc ttgttggttt tgcgtttctg ctcgtcgttt
     3661 tcggctattt tgcgttccag gctgaggtcc atcgccggcg catgcggtgc gccgtaagtg
     3721 gagcgggctg gcgtaaagcg gccctgtggt tttggagcat aatccggctg ttccatacgg
     3781 acgaccctgg ggcctttgag ttgtgcaggt ttgggaatca gcatggggcc ggcgggcttt
     3841 acggttttgc cgcgtttctg aggacgttcg gctgccgcag gtttttcatc tgtcgctgca
     3901 ggttgctgtt tgggggattc ctgttgaatc ggctcaggtg cggtagtcgg ctgttccgcc
     3961 ggctgctcct gggctgtgac gactgccgta gtcggctgag gcggaacttc ttccttaacc
     4021 tccgcgatgg tttctatctg ttctgtaggc tgctccgcgg cggccgggat ttcttcgccg
     4081 gtttgctctt ccgcgacggc ttgggattct tcatcttctt ttttgccgcg gcggcgggtt
     4141 ttctgtgcct cttcgtgagc tttttcaata tctacgcggt cggaggtttc tacggcggta
     4201 ctttccacgt ccgatatgcc ttcactgaac cagtcacgta tggtctcttc aaggccggct
     4261 gaaatgccgc tggatgcagt ctttacaatg tcgtccagcc cttcggctgc acatttgtcg
     4321 ataatagcct tgctggttac gttcagctct ttggctaact catgtattct tttttttgcc
     4381 acagactacc tccgcaaagc ggtattcctg aaaatcatac aacttttaaa tttttcaatc
     4441 aaattgaacg attccgtatt tatccgtgta ttctgcacgg aaatttactt cgtaacaggt
     4501 aaaacacagc ctggctgccg gatatggtca aagtcgcccg acacctaatc cagcccgcaa
     4561 actacaaaag caactcaaac ctgctacata ccgttaaagc cgtcaaacca gtgacatctc
     4621 aaaagcttta aatatcaaaa tataaaaaat atcaaaattt acggcaactc ctgccgttgt
     4681 acctgggcag ccttggtttt ggtttcatcc acgttcttcg aggcttcagt gcatgcttcg
     4741 acgatagcct gcgcaaccgg ctgttctata ccaagttctt ccacaagcgg gtcagggccc
     4801 acttcgtcca catccaggac tgaaaccatg cccatagcta taattttgtc cactattgca
     4861 gggtcgacgc cttcaatgct tttggcggtt ttttcgagca ggtcaagagc tgtgttatat
     4921 tcatccggcg taaggatgtc tatgtcccag ttcgtcagcc ttgcggcaag gcgtacattc
     4981 tggccgcgct tgccgatggc gagcgaaagc tggtcatccg taacaaccac ggtagcccgg
     5041 ccaagttcga aacagagagc tatctcggtc acttcagccg gcttaagtgc attgcctata
     5101 aggtttttcg acgactcgtt ccagcggacg atgtctattt tttcgccgcc gagttcatca
     5161 acgatgtttt ttattcggct gccgcggata cctacacatg ctccgacggc atcgactttg
     5221 ctgtcaaggc tgttgacggc cactttggta cgatagccgg cttcgcgagc cagcgcgttg
     5281 atttcgatga tgccttcgga cacttccggc acttcaagtt cgaacagcgc acggataaaa
     5341 tcggggtgtg tgcgtgaaag aatgattttc acgttgccgg gctgttcacg tacatccaga
     5401 atcatcgccc ggatgcgttc gtcgatatgg tgagaatcgc cgggaatctg ttcgcttttg
     5461 ggcaagatgg cttcgccgcg gtcaaggtcg accacaagcg agccgccctc ccacctggcg
     5521 acgcggccgg tgacgatttt gcctttctgc tgggcgtatt cgtcaaaaat gctatcgcgt
     5581 tcggcttcgc ggatgcgttg aatcagtact tgtttggcgg tctgggcggc gatgcggccc
     5641 aacgcggcga tgctcatggc ttcgccgtcg atgactgcga ttatgtcgcc tgtggcacga
     5701 ttgatggaaa cctctatttc ggtttcggga gtgttcgcct tgcgaatggc tgaacgcatg
     5761 gccatttcaa ggtcgtcaaa caccacgccg gggtcgatgt ttttttctcg tgctattccg
     5821 ttaactattc tcagcatttc ctgattcatg gcaattatcc tgcaaaaact gttgttcact
     5881 aaccggcgag gcgtgaccct cccagttttt ttgggccata atgtcatact ttcagttggc
     5941 cgacagccgg ccaagcatca catatatgaa gtaaagcaaa aaaacaaaaa aagcgggcaa
     6001 acggcccgct tccgtaaaat tcactgtgga tagaatcatc cctttctgcc cctgccgctc
     6061 ttacggcggg caaaagagga tggaatccta cccggaggtg cggaaagatc cgcgccgtcg
     6121 tgggccatca ccatcttgcc atgccgatta tgcatcggcc cagcctcgct gtccaaggct
     6181 aacaatcacc atcgtgattt catgcctgta tcgctcaagt aacacttcgt taatcctgat
     6241 tcccaaaaat ataaaagcag gccgccaaac ggcatcctgc caaatcgatg cgaatccgac
     6301 attagtaaca gtttgggaaa ttttaaaccc ttacattccc gtggtcaata gcaattgtgc
     6361 ttttttcgta tttttaatgt ttatatgata aaaaaattca ttacaatacg ccgtcatatt
     6421 ttcagcggct tatctgggtg ccggtgtagt agtgtagtaa tttatgtggc ttaagtttat
     6481 tggagaattt ttcaaatgat tttcgactct acggataatc tggatttgta ttttgacgca
     6541 ataccggagc ttgaggctgt cgcggaattc ctcgctgcaa tgccggctga cgcggccggc
     6601 agaacaagta tcgaaggcga cgacctttac gtaaatataa atgaatatga aacacggctg
     6661 agaggcgagg gcagatatga atttcatcaa aaatacattg acgttcaggt ggtgctggaa
     6721 ggcagggagg aactcgacgt tttaaatgag acaaatcttg aattgaccga ggcttacgac
     6781 gaaactgtcg acgtcggttt ttacgccaga ggctctcagg ctgccggggc taccctgcgc
     6841 atgatacctg gcagattcgc ggtacttttt ccgcaggata ttcaccagcc gtgcatcgct
     6901 gcaaatgacg agccgtcaaa agtcaaaaaa gctgtcttta aaattctctt ttagagcagc
     6961 ctggcacagc cgaaaaagct ttcgcaagct cattttggaa ataaatttga gaatcgctct
     7021 aaacgaaatg aaatacctga tttgccggag gagggactcg aacccacact cccttgcgag
     7081 aaaaggattt tgaatccttc gcgtctgcca attccgccac tccggcaagg aggggacatg
     7141 ataaccatat cggctgaaca tggcaagaaa aacatatgtc gattaacgct ggccaatgag
     7201 aatggttatt ttttcgagaa ccatatctat ttgggattga ttcgttcgac gatttcagcc
     7261 aagccgctta gcgggtcgtc gatgtcttct atgggttttc tggcagtttg cgacgatgcg
     7321 ttctgcggcg gcaggccggc gtattccggt atcggcgtat tcagaatgtt taccagggcc
     7381 gagtaatttt tgagtatcca ttcgactttt ttgcgtatca tctgcagaaa ttcgcagccg
     7441 ttgaatcgcc cgaaaccgcc ggtggacgca atggggagga cggcttcgga aatgatggct
     7501 tcgcacatta gcagcggaat ggatttcagt tcgtcctgcg ttatgacatt caccgaatca
     7561 tagccatgca aaaatgcctt gaatcgcggc atatcgagat tcgccggcca ttcaagcgga
     7621 ttatcgccgc cgccgatgat tgaaaactgc aacgcgccgt ttgcaaaatc tatgactcgc
     7681 tgatgcagcc gggccgtatc gtaatctatt accgcggcga cgagctggtc gcggaaaagc
     7741 atgttgccgg gatgccagtc gccgtggact atctggctgg gccagtgatg aaggcctatc
     7801 ttgttgagtt tttccacgca cttgcgataa tattcgtcca gaatcgtgac ggtgttgtct
     7861 atggtgttgc tcgcctggtc gcatttgctc ttcatcgctg acgcacagtg aagtattgag
     7921 tgatgtatcg ccttggcatt gtgataactt gccgtcggcg gttcatattc ggaacgaaaa
     7981 tgttcgagca gcttgtgata cagccccagc gcatggccgg actgctcggt cgccttcaat
     8041 gactggtcgt aacctgtgcc ggcgatgaat tcctgcaatt cataaatgtg gtcattgaat
     8101 atgagcatcg aattgccgtc atcgcgggtc ggctgaaggt gcggcagcgg aaaattctga
     8161 gccgccaggc tgagctggat gtgatgcgtg aacgcgacct tgtccagatt ctccacgccg
     8221 tgctgacgac gtttgaaaag atatctgccg cgctcgcatt cgactatgca cttgggagcg
     8281 cggcttgagc cgcgtggaaa ttcacgtatg ctcttgatta tgccaaggtc gtaatgactc
     8341 aaaccgatgg ctaattcttc ggggtcgaag tattccctct ttatcatttt tttatgtttg
     8401 ccgaccatcc ttacctctaa agtctgttcg tgcaaaagtc tccaagggtt aaaacccgta
     8461 gcattgtgta cttttcaggc gtcatttacg gtttgcagta gctcccaaaa cagaattctt
     8521 ttggcaggca ctctttaaaa aaccgttgaa ctacgaatga catattaaaa tactacgcaa
     8581 acattcatta tcgggcaagt ttttttaata ttataatgat acaaatttaa cagagaaacg
     8641 gctcagaaga ttcgatacaa tataaaaaaa tcatgataga ttgtcattta catctccagt
     8701 tggctccatt aaatgcacag atatgtcggc acgttcagtc tgccggcatg aaaggtgtgg
     8761 actctttctg gtgcaacgca acatcctgcg acgactttca ggctgttgcg gaaatcgccc
     8821 tgcaatacgg ccagattgtg ccgtttttcg gcatacatcc ttttttcgta gccgattcgc
     8881 cggcggactg gctggagagg ctggagtcgt accttgagaa atttccaacc gccggcctcg
     8941 gcgaagccgg cctggactgc tggttcaaaa aatgcaaaaa taccctggag cttcaaaaga
     9001 aatttcttgc ggaacagata cggctggctg accgacttag ccggccgatg gcgttgcact
     9061 cgctcaacgc cgatggtgcg atgttggatt tgctggagca gaatacgccg cagcggccat
     9121 tcatgatgca tgcattccgt ggttcgccgg agacggttgg ccggctggca tcgatgggcg
     9181 cttatttttc atttgcgggc aatatcctca acccgcaagc cagaaaagcc cggcaggcag
     9241 tagtcgccgt gccggcagac cggctgctga ttgagacgga ttcgcccgaa ttgaaaattt
     9301 tcggatacga ctacccgact ctgccggagt acctcggcct tgtcagggat gaagtcgcaa
     9361 aaatactcaa aacgaccccg caggatgtgg aaaatctgac gctcgaaaac gccaaaaaat
     9421 ttctcatggg tcaataaaag atttttgggc tgcaacaaaa atacaggatt ttgacatgga
     9481 cagattttta cgaaccagaa tgctgctcgg cgaaaaagcc atggaacgac tcgacgactc
     9541 gcacgtcgtg atagtcggcc tcggcgccgt cggcggctat gcgatggagg gcattgcgcg
     9601 cggcggcgtg ggcaatctgc ggctggtgga tttcgacgtg gtctcgccga cgaatataaa
     9661 tcgtcaactg cttgctctgg attcgacggt cggccagccg aaagtggaat tggcggctgc
     9721 tcgcgtaaaa gacatcaatc cggcatgtca cgtccaagcc atgcgagctt ttctgcatac
     9781 cgatacgctg gacgaaatac tcgccggccg ggtcgacatg gtcatcgacg ctatcgatgc
     9841 gctattgccg aaggtggaac tggcggctgc ctgcatcgaa cgtaaaatac ccataatctc
     9901 cagcatggga gctgcattgc ggaaagatcc gtcgttcata aaaatcggcc cgctgaaaaa
     9961 ggcgacgcat tgtccgctgg cgaggcgcgt tcgcaaaatg cttcgcaaac gcgggttgac
    10021 gacggacatt acctgcgtgt tttcatccga gccgacgtgt gaactggaag aatcggcgat
    10081 ggcggagctt gaaaatagcg aacagggggt aattttgcgc ggccggccca ggcggtcgct
    10141 cggctcattg ccgacgataa ccggcatttt cggcctgact atcgcaaatg ccgtcataat
    10201 ggccctgctg caaaatccgt ccgaagacga aactacagcg ataactccca ataacgcctg
    10261 aagttccgca atcgccgatt ctgcaccaaa aatcgttatt caaaaaaacc attgtccgcg
    10321 gacagacggt tatatacaat attgcaaatc gttttgagaa tacggagaac cggaaatgaa
    10381 gaatgattat aagataggat tggtcatagg gctattggtg ctgttcctgg gtgtgggtta
    10441 ctttattttc agagggccag gcaaaaccag gaagcccgaa ataccgactg tccctgaaac
    10501 tccggcagcg gagaacactt ttgtaggtga aacgacagca accggctcgt cgaccaccag
    10561 cgagtttccg ccgccgagca tgtccggcga gactactccg gcgaataata ccgtcgctgt
    10621 tgcgacagct gcgcctgcgg ctgaaaccgt ttcgccggct gcggcaaaac cggcacagtc
    10681 ttctacgctg gcagagcggg tcgataattg gtcgactcgt accggcaatg aaaacgccgc
    10741 ggaaaactcg ccgggtgctt ctgaaacact gtcgccgcgt ctggcggata acaatgcagg
    10801 tggtactgca tcttccgaaa ccgtcgcagt aaaacctgcc gccggttcat taaaaactcc
    10861 gcccgccgaa gctgccgtcg aatggggtgt gccggcggtc gaatcaacca catctgcaag
    10921 ccaaaataac gcgacgaccg tttcttctgt tgagccggtc ggcggtacta ctccaagtac
    10981 atacgtcgtt aagccaaacg acgtgggctg gtatattgcc gaaaatgttt acggcaaagg
    11041 tatgggccaa tattggaaac tcatccgcca ggctaatcct gacgtcgaca tgtccaatct
    11101 caaggctggg caagtgctca agattccccc gctgccggaa aatgcaagac agcggctgcg
    11161 ggacactgta acgcagggta cgacctttac cgacgcgacc ggaaaaaata tctacgtgac
    11221 gaaatccggc gacagctttt ggactattgc gcaacgggtt tatggcaaag ggtatcgtta
    11281 caaggaaata cagaaggcta atccgggcgt tgagacattg cggccgggcc tgcggcttgt
    11341 aattcctggt gaagtcgtca gcacatcaag cgttgccggt gaaatgacca cttacggaag
    11401 cgatactgta acgactgaaa cttcagccgc gaattactat actgtaaaat ccggagacag
    11461 cctctggacg atagccgaaa aagaattcgg cgacggttct tatgccgacc tcatcaagaa
    11521 agcaaatccg aatatcaacg ccaataatct tcgtgtagga cagagaatcg ttctgccagc
    11581 caagccggaa ggcacgcccg ctacgtctac ccatacgtcg gacgaaaaca ccgcggaacg
    11641 tcgtcattcg gcgccttcgt cgtctgagcc cgttccgtcc gatgaaccgg atttcgggtt
    11701 atgatgcgat attgaacgac cattcgtcgc cggcgatttt cgaaagcaga aattcgccgg
    11761 cgattttttt tccgcggagg ctgacccgca aaccgtcggc agtttccgcc ggctcgcaga
    11821 agcctttgtc tattcgcgtg acttcgccgc gacgattgct gacatggccc tgatatgtga
    11881 gataagcctt gcgatgcggc ggcagttgtc tgcagatggc gccggcggct ggaaacgata
    11941 gaaactgcca cgttgccaaa cgctcaccag tctcaatcat caggtcgtaa tgatcctgcc
    12001 cattgccgct gtgaaacaat atgacaaatc tggcttgcat gattttttaa aataaattca
    12061 aactgaccgg tctaacggtt tttagtatac cgaaccaaat atcgtcaatc gggcactttg
    12121 agtaataaat aatagaccct tcgtaatttt tgggagttaa cccgcaaaat tattactgga
    12181 gcatgctctg aaaagtcagt ctttatcgga tgatttcgat tccggagagt cggcggtttt
    12241 gtcatctttc tgcaccggca ctatttttac ggtcagcttg ccatcgtcaa ttttaaagtc
    12301 gcgtatctca cgcagtcgtt ttgagcccag tttgaattcc gtcggaaaag gcttatcgtt
    12361 gattttagct atcacgttcg cgattatgga atccaggtct cgcagagatt cttcggtaat
    12421 agcgtcctgt gggccgacgg ctgttttgaa tgtattgatt gagccggcga cgagcgtctg
    12481 cggcacggcg agcttgccta ttctgacgct ttttattgat attagaagat gcctgtcgtc
    12541 cgtcatgtca aagcctatat ccaccgatat gaccttgttg gcggctctgc ttcgcaccat
    12601 tagagtcatt acgccgtcgt caagccgcac agccgggtcg gcaaagccgg cggcgtccat
    12661 ggcttcgtaa acgcccgacg tcttgcgttc ctggttgcgt tttgcgggtt tgagaaatgc
    12721 tatttcctca aggctggcga aatagtagtt aagctcgtct tcggtgatgg tgtgttcgaa
    12781 tggttcaggg cgacgaatct gcaccagcag cggcgcgccg tgtttgttga tgaaatcctg
    12841 ggcggcttct ttgcgttcct gctgcgtaag ctggaacggc ccgtaatcgc ctggaacggt
    12901 actcgccata aggaacagaa tcgccaataa cagcataagc gcgagtacga ctcccgtaat
    12961 tatgcgtacc gtttttttac ttacagccat cgtagcctcc tgaatatctg cgaatcaata
    13021 tagcccatgt gttctttaaa atcaacagcc gatgccgttt ctgtccgtct tctgatgcct
    13081 gtagaaacgc gatgtcggca ggcttatccg acgttagagc attgaaagat tgagagttca
    13141 tatttttgcg tcagtgggtt tgttcgtggc aaggaaggaa aatgaggcat acttggcagt
    13201 attctgaagt ttttctgacg cagccacgga caaagcaaac cagcaaaaaa ggaactctca
    13261 agattgaact gctctagacg caaaacgcct gcaggaacgt atcgaaacct ataaagcacg
    13321 catctgtttg tattcgtcga ggccattaaa aatttaaaaa aatcggaaaa aagttgcaat
    13381 ttggaacgga aacagtacat tattgttctg tttctggtta tttaaactga ccggaccgcg
    13441 tatccgtgtg ggattacggc cggctggtga ggacacgaaa atgtcaactg tagcaaaaga
    13501 agttaaggaa agtatcgtat cggaaaatcg tattcatgaa ggcgacaccg gctctgccgg
    13561 cgtgcagata gcccttctga cggcacgcat caaccatctt gccgagcatc tcaaaagcca
    13621 cgtgaaggat cattcttcgc gtcgtggctt gctgaaaatg gtcggcaaac gcaacagcct
    13681 gctgaaatat ctctcagcca ggcagccgga gcgttatcac gaacttatcg ccaagctggg
    13741 tctgcgcaag taatccagcc ggctgaattt tcaaggccag gcccgcacgg cagggaatgt
    13801 catgcgggcc tgactggttt taacggacac tggaaaccgt taaataggtc tgtttgaata
    13861 aagttaaaaa atcaaattga gtaaaaataa gtaaaaaaag agttaacgaa gagtagtagt
    13921 aacttgagga caaggaaaag tataaggacg aaaaaatgag tagcttgagt aaagtagtga
    13981 aagttcagaa agagattggc ggcagggtgc tgtcattcga gatgggtaaa tgggccaagc
    14041 aggccaactc cgccgtggtt gttacttacg gcgagacggt tgttctggcg acggttctcg
    14101 tcggccctgc gggaagcgac atagattttt tcccgctcta cgtggattat cgtgaagcac
    14161 aatatgctgc cggcaagatt cctggcggtt ttttcaaacg cgaaggccgg ccgtccacga
    14221 aagaaattct tacgatgcgc atgatagacc ggcctatccg gccgctgttt ccgtatgatt
    14281 atcgctgtga agtgcagatt cagtgcatgg ttctcagcag cgacaatgaa aacgaccccg
    14341 acctgatagc catgataggc gcgtctgccg cgttagccgc atcgtcggct ccgttccagg
    14401 ggcccatagg tgcatgccgc gtgggttata ttgacggaca gtacgttgtc aaccccacat
    14461 tttcgcagct ggcgcagagc gaaatggaac ttctggtggc tggcccgaaa gaagctatca
    14521 acatgataga gtttaccggc cgtgaaatgc ccgaagacgt agttgccggc gcaaccgacc
    14581 tgggccataa aatcgactgc gatattattg cactgacgga agaattcgtc tcgcagcttg
    14641 gtgtctgcaa ggaatatgaa ccgaccgtca tgcccgaaga gctcaagaaa atcgccgcag
    14701 agcgatgctc ggcagctatc cgcgagaaaa aactcattcc tggcaagcag gagcgtaacg
    14761 aggcgcttga cgaagttcgt caggcactga tagacgaact catccctgaa ggcgcagccc
    14821 ccgaaacgct gccgtatacc gaagctcaga ttaaggaagt tttttacaaa cttgaaggca
    14881 ggatacagcg ggagctgatt ctcgaaggca aacggcccga tggccgcggc tatgaggata
    14941 ttcgtccgct gtatacggaa gtgaacgtgt tgccccgcac gcacgggtca gccatcttcc
    15001 aacgcggtga gacccaggcg ctggtgacgg ccacgctggg aacgccgcgc gatcaaccca
    15061 taatcgacgg cttgcaggac gagtacaaga aaccgttctt catgcactac aatttcccgc
    15121 cgttcagcgt tggtgaaatc cgcccgctgc gtgggccggg ccgccgcgaa atcggtcacg
    15181 gtgcactggc ggaaaaatcg ctcgaaccga ttatgccgtc gaacgaggtt ttcccctaca
    15241 ccgttcgact cgttgcggac attcttgagt ccaacggctc gacgtcgcag gcagccacct
    15301 gcggcggcac tcttgcgctg ttagctgccg gcgtgccgat aaaagcaccc gtagcaggca
    15361 tcagcatcgg catggtcagc gacgatgaag ccggcaaata cgtgctgctg actgatatcg
    15421 tcggcgaaga ggattttcac ggcgatatgg acttcaaagt tgccggcaca aacaagggca
    15481 taacgggcat tcagcttgac atgaaagccc gcggcataaa gcaggacaga atcgtcgcca
    15541 catttgcgca ggcccgccgt gcaagattgc gaatcctgga agccatggct gccacaatat
    15601 ccgaaccggc gccaatgagc gattacgcac cacgcatgct cacggtcaag attgaccccg
    15661 agaagattgg caaggtgatt ggccctggcg gcaagatgat aaacaagatt caggacgaga
    15721 ccggcgcgac gatagacatc gaggaagacg gcacgatttt cattgcctct tcaggtgaag
    15781 gcgccgagca ggccaaggcc atgattcagg ctatcaccga aaatcctgaa atcggcaaga
    15841 tatacaaggg ccgcgtggtc ggcatacgcg aattcggcgc gttcgttgaa atcctgcccg
    15901 gccaggaagg cctctgccac gtcagtgaaa tgtccgacga atacgtcgcc gatccgaacg
    15961 atttctgcaa actcggcgac gagatggaag ttcgaatcat cggcattgac gatactggca
    16021 agataaaggt ctcgcgcaag gctgcgttga atcctgactg gacgatagac gacgtcaagc
    16081 cggcgccgtc ccgtcgcggt agcgacagag gcagccgtaa cggcggagaa cgtcgtggcg
    16141 gcggtagcca tcgtaacggc ggcgatagaa accgtcgtga ccgcgaatag tatttaatga
    16201 ctgagtgctg atttcttaaa aagaagtgtt cacgagacgg gcctggtttg ccgtaaggcg
    16261 acaggcccgt ttttttatga accgctataa tatgctctaa cttggtttgc agtagtcgat
    16321 gattctcgct atttcgctgc gaagctgatg acggtgtaca atcatgtcca cgaacccttt
    16381 ttccagcaga aactcagccg tctggaaatt ttctggcagt tccgccttga ttgtgttcgc
    16441 gatgacgcgt gggccggcaa aaccgattag cgccttcggc tcggcaatta tgacgtcgcc
    16501 gagcatggcg aagctggctg tcgtgccgcc ggtcgtcggg tcggttaata ccgatatgta
    16561 caatccgccg gcctggtcat agcgagcgag agccgcggat gtttttgcca tctgcatcag
    16621 tgacagtgcg ccttcgtgca ttctcgctcc gccggaaatg cagacgacca ccagcggcag
    16681 attttccttg gtggcaaact cgatgagcag tgagagtttt tcgccgagta ccgagcccat
    16741 cgagcccatg aggaaatcgc cagccatcac tcccagtgca acacggcggc ctttgatgaa
    16801 tgaaacgccc gtaagtatgg aatcgtccag gccggttttt ttctgctcgg cggccagccg
    16861 ctcggcgtac tgtttgcccg accactcaaa cttgagcggg tcggcgggtt tgatatccgt
    16921 aaaaaattct tcgaagctgt cttcgtcggc aagctgggcg attctctggc ggccggtcat
    16981 gcggaagtga tactggcatt ctggacatac ttcctggttc tcggttacgt tctttctata
    17041 tagtgtggat tcacaagccg gacactttag ccagaggccg tcgggcagat cttttttcac
    17101 ccgttgcggc gggccggaaa gggtcgatgc gtcaaacgcg atattctttt cctgttcagc
    17161 cgacaaactg tcattctgaa tagccataaa acaaagctcc gattttccaa tttttcaata
    17221 tgtttttaga ggctgttctc gattaacctg gcttttaaag cctttaccgc cgcccctggg
    17281 tcttgcgtcg caaaaacact attgcctgcg acgaatacgt cagcgccagc cttgtgtgct
    17341 acggttatgg tcgaaacgtc tattccgccg tccacctgga gtatctgatg cggtttgagc
    17401 atgtttttga tttcggacac ttttggcatc tggtcagcca tgaacgattg gccgccaaag
    17461 cctggttcga cggtcattac gagaatcatg tccaccaggt ctattatcgg ctgaagcaga
    17521 cagacgggcg actgcggctt gattgtaacg ccgacgccca gattcatttt gcgtatgcgt
    17581 tctataaggc gttttgcttc gtcagccatg cccgtgcccc agcggccgca ggcttctatg
    17641 tggaaattaa ggctatctgc gccggctttt gcgaacggct cgaagaaaaa tcccggatcg
    17701 ctcaccatga gatgaacgtc cagcggggcg tcggtatatc tgcgaaattt ttccacgacc
    17761 ggcacgccca ttgaaaggtt cggcacgaaa tggccgtcca ttatgtccat gtgcagcatt
    17821 tctgctccgg cggcgagcac ctggtctatc tgttcgccgg cgcgggcgaa atccacactc
    17881 agcagcgacg gcgctatcgc agggccggct ggcagagatg tccacatatt ctgtcccatc
    17941 tgtaactcca atacgtttat ccgaccgacc ggctgtgagt ttgtctcgat gtcattacgc
    18001 agcctgcgat gtgcggcgtt gacggcatgt tccggctgcc gtgaaacatc ggcagactta
    18061 ttgcacattt tcagcaggaa ataattttcg agaaccgctc tgaggccagt ctgtcaatcc
    18121 gacgatttta caggattttt tagcctgcgt ccagaaggat atcgtttcca cggcggcgat
    18181 tttgggttaa aatcggcatg aatgagacgt gatgaaatac ttcagatgga tgacgaaaat
    18241 cttattgccc tttgcgatat gcacatatac aaggcatccg gacctggcgg ccagcatcgc
    18301 aataaggtgt cgtccgccgt acggctgcgt catcgcgaga cgggcataag cgcaacggct
    18361 aacgactccc gcagccagca tgaaaacaag gtgttagccg ttaaccggct gcgaatgaat
    18421 atggctttgc ggttgcggga gaatgtggat attgttaaac tcgttttgcc gacgtatttg
    18481 gtcggctgca tttttaagcc caaaaaacct cgcgaaggag ccgccggcat gcgcctggaa
    18541 ataggccgaa aagacaggcg gttctggccg acggcggcat ggctgctgga tttgctggtt
    18601 gcctgcggcg gaaaaatagg cgacgcagcc aggctgctga atatttcgac gggaaatttg
    18661 acgtcgatat ttcagtctga ccgccatctt tgggcagctg tccaggaaat acgcagggac
    18721 gcggggctga atcatctgac ctgaaaaaat gagccgaaaa gacagacata tcgaattgac
    18781 ggaaaaaggg ttcagcctga ttgacggcgg tcaggcggct gcggaattcg attggataca
    18841 cgtgacggaa atagtggcgt ataggcgtga cccggaaggc gaggatctgc tctgtctggg
    18901 gttcagggcg gctgcctcgc ggaattacgt ggaagtggac gaggagacgg acggttattc
    18961 aaaattgctg gctgcgatgt atgaagcgtt tccagccatc agccgtgatt ggtggcagga
    19021 tatcgcccag ccgctcggaa cgaatcgaga aactatatac gggatggcta tgagtgagga
    19081 gaatgaagag ccggctgcta gcaagtattt aaagtcgctg agcttacaaa aaaaacagtc
    19141 ccgcctgcga cgtcggctgc tgctggttat gttatcggca gccgttgttt ttggcataat
    19201 tttgggatta ttgtccgccc gaagttccgg cggctggctt gtaatcgttg gctgtgcgat
    19261 tcagtttatc gtttcaacgg cagtgctccg cagaataaaa tcagccgccc tgacgctggt
    19321 attgtttgcc gtttcatact ggctggcggc gtttctgtgc cgctgacatg acatcaaaag
    19381 ccgttattcc tttaaaaata atgaattaag gtggcaggcc ctcccggaaa ctgtgtaaag
    19441 gccagatttt tatcaaggaa cttccgtcgg ccgccggcga gggccgtttt ttgactgcaa
    19501 cgccgcaatt aacttgactt tacgtggggc taaaactata ctctctcgac ttgatgcagc
    19561 tgcgcaagtc acaataacgg tatttgatgc gccctttgca aattctgcag ctgttgggct
    19621 gtagatggac gcaaccccga ggcacactgg cgaaagcctg aagcatgatg ctgccggtta
    19681 tataaccagc agcaatttat tgtcttaaaa catattttta gggttttacc ggaggtcaga
    19741 cagtggcaaa aaaatcctca tcgaaagctg tcaaaaaaac tgccccgaaa gctgcgataa
    19801 aaaacgctgc aaaaaaggca gtcaaaaaaa gcgttaaaaa agccgcccct gctcctgcca
    19861 aaaagcaggc gtcgaaaaat gtttccaaat ccgcaaaatc aggcaacagc agcagttcca
    19921 caaaaagcat ttcaaaaaat aaaaataacc aggcagtaaa aaagataaaa aatatggccg
    19981 taaataacaa attatccaaa aaagcgacca ataaagacaa agtcaaatca gttctcagca
    20041 aaaaggaact ggaagaattt cgccggatac ttattgaaaa gcgcaagggc attctgggcg
    20101 acatggctgg aatgcggggc agttcaatgg gcatgaatct gcaggattcc gccggcgacc
    20161 tttcaaatat gcccacgcac cttgccgata tcggctcgga taatttcgag catgaattca
    20221 cgctggaact gcttgagagc gaacgcgacc ttttgcggga aatcgacgaa gcgcttgacc
    20281 gcatagacga cggcacgttc ggcatatgtc tcggcaccgg ccagcccata agcatcgccc
    20341 gcctgaaggc gcggccctgg tgcaggtaca gcatagagta caagcggatg atagaaaagg
    20401 gcttggctgc accgaggcat tcgtctgatg acgaagaaca tcacgaacga gatgacgaag
    20461 aataataggc tataaactca aaaaacgcgg agcgcacttg cctccgcgtt tttattttgt
    20521 acactcacga ttatggaaga ccgtaaaaaa aatcaaccag cgtataaaag cgccagagcg
    20581 tggacgatat ttttatccat cgccgtgctt gccgccgccg cagacctgtg gtccaagcat
    20641 gccgtttttt catggctgtt gaacgacaat tcgcaacagc tctcgaacaa ggctgccaat
    20701 gatgccgcgt atttgaagga gtgtcattcc gcggaactgc cgccggctga cagcgaggcg
    20761 ttttcacgcg tggtgcttca gcggctgcac atacatcgtg acgtttgccg cggcttgcga
    20821 ttcacgctgt cgacgaaccc gggcgtggtg ttcggctacc gcggcatgcc tcggtacgta
    20881 gtcaacattt tcaccgtcgc gatgatgttg gcggtctgca tgttcttttc gttctgcagt
    20941 cgtcgcgatt actggttgat aaccgccttc gcactgatac tcggcggggc ggcaggcaat
    21001 ctgtacgaca ggctgttcag cgtcgtgagc atttcgccgc ttgtgccgat acgttttcac
    21061 gtgcgcgatt tcatcgattg cagcgatatc ggctatcaat gggttttcaa tgtggcggac
    21121 gcgtggctgg ttatcggcgt tgcgatggtg ctgctcaatt cgctttggct gtggcggaaa
    21181 gagatgcgaa ataaaaacgc aggagttgcc gcaaaatgaa aattttcctg gcaggcataa
    21241 tgcagggctc agtcagggac gacaccattc atccgcagga ttggcgcggg ccgatacgcg
    21301 aaataatttt gcgacgcata cctgacgccg aggtttattg tcattattcc gaacacccga
    21361 attcactgac ttacagcggc ggaaaaatcc gcgagacttt tgacgacggc gtacgacgtt
    21421 gtgccggcag cgaccttgtt atagcgtatc tgccgtctgc gtcgatgggc acggctattg
    21481 aaatgtacga ggcataccag aatggcgcag tagttctcac cgtcacgccg atggctgcca
    21541 actgggtggt acgactttac agccatgccg tatttgccac gctggaggag ctggacgttt
    21601 ttctggcgga cggcaggctg gaggcttttt tctgaaatga tacatattct ggacattacg
    21661 ccggaacgtt tttgtgagca catcgaacga atcggcgaaa aaaaattccg cgccacgcag
    21721 attttgcaat ggatatggca gaagcgagtg acggattttg ccctgatgag cgatctgtca
    21781 aaaaaattac gggaaaacct ttttggggag ttcgacatca ttaccggcag tatcgcagca
    21841 cgccgcgaca gcggagacgg ctgcgtgaag ttgctggttg aatatcccga cggcgaacga
    21901 atcgagactg ttgccatacc tgctgaagag cggcttactg cgtgcgtttc tacgcaagcc
    21961 ggctgtgcaa tggcgtgcag tttttgtgcg tccggccttg acgggctgcg gcggaatctg
    22021 acttccggcg aaattgtcca gcaggttctg ctgctcgaac aggcaaccgg ccaacgagta
    22081 acaaacgtgg ttttcatggg catgggcgag ccgctggcga atcttgatgc cacaataaat
    22141 gcggtacgaa cgttagccga ttcccgccgt ggtgcaatat cggctcgcaa aattacggta
    22201 tcgacctcag gccttccgaa gcagatacgt caacttgcag aactggaact gcccataacg
    22261 cttgccatat cgctgcacgc tccgaacgac gaacttcgtc gaaaacttat gccggttgcc
    22321 tcgcgatacc ccatcgccga aattatctct gccgccgctg agttcttcaa ctcgcacaag
    22381 cgggaagtca cgctggaata tacgcttatc ggcggcataa acgactcgcc gcaatgtgcc
    22441 gcacaactgg cggcgatagc agctacgctc cgctgcaacg taaatctcat acgttataat
    22501 cctgtaagtt ccttgccgta tgtaaggccg gatgaacggg tggtaaatga ttttgccgaa
    22561 ctgctgcgcc gcaaacacgt caatgtcacc gttcgccgca gcaagggtat cgacgctgat
    22621 tccgcgtgcg gccaactcag aaggcgaaac gcctgaataa gtaggatttg cattgccgtg
    22681 tcattttctc cgattacaca tttcgagaat ctttttaagc catttttaaa gctgtttata
    22741 aatgtattat ttccggccaa gccttattgt agcttcgcaa tgggggcagt tttttaaaaa
    22801 gcttttcatt attttgaggt tttccggaat tttctggcat tagcgaacct aaaatatatt
    22861 atgacagtaa tagtcggtac aattcgagat aggggtactt caaacgtgaa attggtgact
    22921 gaccagacaa aaagcacgga cagaaacgcc gaattaatgc ttcagttcaa agccggcaat
    22981 cacggggcgt tcgaagaact cgtccgtaga aatcttggca acgtccactc ccttattttc
    23041 aggttcatgc gcaacgaatc gcatgtggac gatttgaccc aggagacttt tttgcggata
    23101 taccgccacg ccgagtcata tcagccgacg gcgaagttca gtacctggct gtacagaatt
    23161 gtcgcaaacc tgtgcttcaa tgccatcaga gccaaaaaat atcaacgtac atattccctg
    23221 gataatagta acgataatga tgtttcagtc gacctgcagg acaaggctca acgtacgccg
    23281 agtgaaaaac ttaatgatga cgaactaaac agaataatca aaaacgccat cgacgatttg
    23341 ccggaaaatc aacgcctggc cattattctc aagcaatacg agcaactgaa ctacgacgaa
    23401 attgcaaatg tgatggaaat ctcgcagtca gccgtcaaaa gcctgctaag ccgcgcacga
    23461 atgacactgc gaaccaaact cagccgatat ctgactgcgt aaaattaggg taatcgccct
    23521 atatatattt acggtttatg ctctatattg agggaaaata gggcaaaaaa ccgttttttt
    23581 gacttttttc tgcgaaactc ttttataggg gcaagtgtta cacagacaga taaatcaggc
    23641 aatctgaaat aaggaatttt ttatgaatac gaatgatttg gataatatat ctgcctggat
    23701 ggatggcgaa cttgatgctg aagaatccga gaaagtacgt aaaattctgt ttgacgatgc
    23761 tcaggcagcc atgacttgtg cggattttcg gaaactcgac aaatttatga agcgatggga
    23821 aacacccaag ccggcggaat ttttaggcga cgttatttgt cttgccgctg agcatgatcc
    23881 agccaaaagc cctttttacc gtataacgcg attagccgtt ccgctgacga tggcggctat
    23941 cgcggtattg gccataatga tgtgggtggg cggggcgcgt tccactgacg atgccgccag
    24001 cctggcatgg agtcatctta cgccggctca gcagtcgcag gcatcgcgca gtgccaaagc
    24061 gtttctgctt ctgccgcagc aggagcaggc aaaaattttg gctgaattcc aaaaaagatt
    24121 caatatcagc ccttcacaga atgattgggt cggtcccgtt ctttcgtcgt tcacgctaca
    24181 ggagcgggat gaacttctaa aactctcgcc ggtcaggcgg ctggaaattc ttcgcaagcg
    24241 gcagttggaa atggcggctt ccggcacaat atcgcaaaaa attccacagg aatgattttt
    24301 taaaaaaata tgccgccatt tctcttccct ggcagcgcct gtctgcattg caatcaacgc
    24361 aggattgcct gtaactttta ttcgtagttt gcaatttttc ccgataatcc tataatcgct
    24421 aaaatgaatt gcgtttttgg aaaatggagc atttaaaagt tgagagttcc gatttttacg
    24481 gttgtagtaa ggaagggaaa ttaggcatat ttggtgatat tccgaatttt tatgacgtaa
    24541 tcacggacaa agtaaaccgg caaacctgaa ttatcaaggt tgaaatgcga taaggagcag
    24601 aagcaatgga agaagataaa aacatcaaca gcctggaaga catctggccg ctgcaggcgg
    24661 cgttaaattc acgtgcagga ttcgataccc tcgctctcgg caaatcgctc aatactgatg
    24721 agaccaatat ggaaatccgt ctgcatgtag gccgcgccct gaaaaattat atcgacgcga
    24781 tggcctccga atgcaatgaa cttcaagact gtctcagttg gaaacactgg tacgccgaag
    24841 cgaaagccgg caggcaatat caactccagg atttgcagaa cgcccgcgtt gaagccaccg
    24901 acatgctctt tttctggata agcatctgcc agctcctggg cctcgaatcg gaggacgttt
    24961 atcgtctgta tgagaaaaaa ctcggcataa accacagacg gcaggatgaa aatcgcaccc
    25021 aggcggaaca tggcagccat gaggatgaaa atcgaaaagt cctatagcct ataagaaagg
    25081 ttcaagtaac aagaaacaac ctacaagcaa aattcaaatt ttaagattca agagagactt
    25141 ctttaaattg aaatttgagt ttttaagatt gcttgtaggt tgtttcttgt tacttgaacc
    25201 tttttacaaa ctatttttta aatttttccg ggtctacgtt cagatttttt accttataac
    25261 gcaatattct cggcgtcgtg ccgagttctc tggcagatgc ggccatattg cctcgcgttc
    25321 gtttcagcga atcgacaata atgtcacgct cgaatattgc caccttttca tcgagcgacc
    25381 ctgttccgaa agtacccgtg atttccggcg tctgcagcga cggcggcaga tgatggccgt
    25441 gaattacgcc atccgtactt accaacacgg ctcgctcgat gcaattttcc aattcgcgca
    25501 cattgccagg ccaatgatac tgtaccatca tgttgattgc cggcgtactt attctgccga
    25561 ccggcttatt gaactgcttt gcatattttt ccacaaaaaa atcagccaac agtaatatat
    25621 cgtccttccg gtcacgcaac ggcggcagga aaatcggaaa aacgtttatg cggtagtaca
    25681 aatcctgtcg aaaagaaccg tcctcgatgg cttcctcaag attgcggttt gtggcgcaga
    25741 taatccgcac gtttgcggtt ctggtctggt tgctgccgac gcgttcatat tcgcgttcct
    25801 gcaatacgcg cagcagcttt atctgggtgc ttggcggaaa gtcaccgatt tcatccagga
    25861 acagcgtacc gctttcagcc tcttcgatac ggcctttacg tgtctgtatt gcgccggtga
    25921 acgcgccctt ctcgtggccg aagagttcgc tttccaaaag attttcgttc agtgcggcac
    25981 agttgacttt tacgaaagag ccttccgccc gcaacgacga ataatgaatc gcgtgtgcga
    26041 caagctcctt gcctgtgccg gattcgccgc gtatgaccac cgtcgtatcc gcctgcgcga
    26101 cctggtgaat ctgttgatag acttctcgca tcaggccgct gttgccgacg attttctccg
    26161 gccgccattt atcggctaat tcactgcgca gccgcaggtt ttcctcttcg agactctggc
    26221 gcaacgcctg actttcgcgg cgggatttga cgtcttcggc tatcatgctg gcgacgatgc
    26281 tcaaaacccg ctcgtccatt tcaaggtttt cgctggggtt gtataatttg tctaccgaaa
    26341 gcgtacctac ggtttcgttg tcaattttta tcggcacaca aataaaactc agctcctggg
    26401 tcagattggc gcggcgttga tgaattctgt ccaggaaatg cggctcttgc gaaattttag
    26461 ggactatggc gcttttgccg gtagctacga cctggccggt tacgccttcg ccgcgacggt
    26521 aacgtatctg tcggcctttg ttggcgacaa agtccgtgcc aatctcggcg ataagctcct
    26581 ccccatcggc ggaaagcagc atgactgttc cgcgatgcat gtccagctga gtactgagta
    26641 cgtccagggc ttcttccagc atttgctgct gttggcggct cgcggaaagt acgttggcaa
    26701 tgccgtacaa ggcttccagc tcacgtaaat tcttctcata agtcgcatct gatatatcgg
    26761 tagtcatcag gtaatcctaa aaaaaattcc aagacaatct tgtcgcttga cttcatgcac
    26821 gtgtcatttt tgaaaacagg ctcatttttg gaacatattt gtaatcaata taattataag
    26881 ctacataatt gtaattttat tcaattttac catctttttg gtaaatatct ttaacgtctt
    26941 taaaaatatg tagttataaa ataaataatt ctatggcacg ctttgtgcaa ctcttcatgg
    27001 caggtcttcc ggtagaaatt attgcaattt acagaccaga cagatttgta aggagtcaaa
    27061 aaatgacgcg acaacatcga aaatgaaatg ccggccagcc tgtgtacggc tatcagccaa
    27121 tagggctggc aaaccggcgg gatgaagtga tcgtaatttt ccagattgat accaaatagc
    27181 aaaaagtcag gattttattg aaggagtttt acagatgcga atatttgcaa gaaagaattt
    27241 gattctgccg gcatcggtat ttctgatgct gtctgccgca ccgtgttttg ccggcgacgc
    27301 tccggctgcg actgccgaca gtgtgcagtt gaatctcaat atcgtatgga cctgcgtagc
    27361 cgcgtttctg gtgtttttca tgcaggccgg ctttgcctgc gtagagaccg ggtttacccg
    27421 cgcaaaaaac gcggtgaaca tcatgatgaa aaacctcatg gatttcgtgg cggcgacgct
    27481 ggtgtttttc ctcgtgggct tcggcctgat gttcggaaaa agtaacggct tttttggcac
    27541 tacgctgttc ggcatgagcg gcgtggagcc tggcgatagt tgggactgga cgttcctgat
    27601 gttccagacc gtctttgccg gcacttctgc aaccatagtt tccggtgcga tggcggaacg
    27661 caccaagttc gtcggctatc tgatatacag tgcgttcatt tcgctgctga tatatccgat
    27721 attcggctca tgggcttggg gcaatctgct gattaccgac aactgttcat ggttggcgaa
    27781 catgggcttt catgactttg ccggctcaac cgtcgttcac agcataggcg gatggctggc
    27841 gttagccggt gctgtagtcc tcggcccgcg tatcggcaag tatcggcccg acggcaagcc
    27901 ggccgcaatc atcggccata atctgccgct cgccgcacta ggcgtgttca tattgtggtt
    27961 cggctggttc ggattcaacc ccggcagtac gaccgtcggc agcggtctga taggatacat
    28021 agcgataacc accaatctgg cagctgcggg cggcgcactt gcggccatag ctgtagcgtg
    28081 gggaattttc tccaagcctg acgcctccat ggcgttgaac ggtgcacttg ccggcttggt
    28141 agccatcact gccggctgtg acacagtaag cccgctggga gccatcatca tcggcctttg
    28201 tgcaggtgtc ctggtagtgt tggctgtgac tttcatcgac cgtatactta agatagacga
    28261 tccggtcggt gcggtcagcg tacacggcgt atgcggagca ttcggcactc tggcggtagg
    28321 cctgttcagc atgagcgacg gcctgcttta taccggcagt ttgaaattgc tgggaaccca
    28381 ggcgctgggc gtagctacgg cattcgcatg ggccttcgta ctgggcatga tactcttcac
    28441 agttatcaac aagaccatag ggttgcgagt cagccgtgaa gaagaactca gaggcctgga
    28501 tatcggcgaa cacggcatgg aagcatatgc agggttccag atattctcca acatctaacc
    28561 cgtaaactga aatcacaaac aggagtcaaa taccatgaag ctgataatag catacattca
    28621 gcccgaaaga ttgaacgacg taaagcaggc actatatgcc gatgagatac acaagatgtc
    28681 cgtaagcaac gtcatgggct gcggacagca gatgggctat cacgagacgt accgcggagt
    28741 caggctggaa gtgaacctgc tcaaaaaggt aagactggaa atagccgtca atgacaaatt
    28801 cgtcaaacca accatcgacg ccataataaa aggcgcacga accggccata tcggtgacgg
    28861 caaaatattt atcgtaacgc tggatgagtg catccgtatt cgtaccggcg agaccggcgg
    28921 tgaagcaata ggctgaaaaa tttttgtcgt gccctgcgtt gtgccaatga cgacgaaatc
    28981 cacggtaagg atacacgccg ataaagaaaa ccgacaatca cttctccata aaaaaggcat
    29041 ggtggaacac aatgtgtttc gccgtgtctt ttttacttgg cagtaagttt ttgagaaccg
    29101 caataaaaaa tacaagcatg agatatggcc atactgctgc ttgcggcatc gacttggtga
    29161 cgtcaagctg tcggcttcgc tttccttgtc agtattgttc tcgcggacgt attaaagcgt
    29221 agtctcaaat tacatatgtt ctggctgtca gcgttttgcg ataaacgcga gctggttcag
    29281 gccgactctg ccccacagaa cggtcagtgg ttgagttcct tttccgtgaa gctgccgctg
    29341 aaccttgtcc gcattcaaat ccacgccgcg ggttttgagt tctacattgc cggcgttgag
    29401 atttttcagc gcctttttta tgtcgtccag tcggcctggc acggattgaa taatttcaaa
    29461 aatctggcca gccgtatcag tcgccggttt tttgtcggca aataaccaca ccaatcctgg
    29521 tgcaagtttc catgccgata atttcatcgc aagggcgctt tctgcgcctg ctgcgagtac
    29581 ggcggcatcg ggttctatga gatatttacc agccgcagcg ccgtatgcag ccggcccggc
    29641 tgcggcgaat accgaatcga cgcgataatc gggatattca ccgcggataa cagttgcaag
    29701 acggcttcct tcgccgacgg ctggaaacca cgcaagcagt tggcgacagg tgttatgtgt
    29761 gctgacgtac tctatatcca gcggcataca gagagccgcc agttcgtctt tcggcatggc
    29821 gggcgagagc ttgatgacgc cccgcaggcc tggctggcgt ataagttctt ccacaaatcg
    29881 cgggcctggc gaataatcgg ctaacgatgc gctgcggcgg ccggtaacgc ggcggtcggg
    29941 atctaaatgc acaacactgc cggctgaaaa attttttaac aaagtcacgt cggcattatg
    30001 gaacgtgcaa catttttcac agccgcaaac ggcagcgtta tatgatgcgc aaaaaaccgc
    30061 agccgacgat
//
LOCUS       DAONFI010000002        21433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_2518_length_21433_cov_4.536720, whole genome shotgun sequence.
ACCESSION   DAONFI010000002 DAONFI010000000
VERSION     DAONFI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21433
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2518_length_21433_cov_4.536720"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="PLK08_00160"
     CDS             complement(<1..1541)
                     /locus_tag="PLK08_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPS51944.1"
                     /translation="MSKIPATQCAVQLVGPDQLRLNREKPVYKPTDHQILAKVEAMGL
                     CFSDLKLLHQFSEHARKGEIVSGIDGAILDEYPAYQPGDKPTVPGHECVCRIIEIGSK
                     VKKHKVGDRCIVQSDWRKILTAASNGAFGYNFEGALQEYILFDERIVIEPGTGDKYLI
                     PVDSECNAAAAALVEPWACVECSYVNVERQTPKAGGRMLVVVDAGRKVKGLAEAMTAG
                     KPAMIDAIGVSAVEGLSVNPLCCCGELASEAYDDIVYFGTKAETIEKLNDALARNGII
                     NIVLGGGKIDREVEVGVGRLHYGLTRWIGTAGDNAADSYKIIPANGEVKAGNSVIVIG
                     AGGPMGQMHVIRALCLGFNVRVVGTDFDDGRLAALSAKANPLAAKNGCEFEAVNTGKT
                     PVEAGFDYYSIMAPVGALVADAIVHAKPGALINIFAGIPAPVKQVLDLNAYISKGLFM
                     FGTSGSLIGDMIIVRDKMESGLLDTGCSLDAVSGIAGAIDGIEAVKNRTMAGKIVVYP
                     ELSNML"
     gene            complement(1690..3747)
                     /locus_tag="PLK08_00165"
     CDS             complement(1690..3747)
                     /locus_tag="PLK08_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS51945.1"
                     /translation="MAKIEWPDFKGETAIGALVKLSHFYGSDPAIVLAGGGNTSGKID
                     GKLYVKGSGHSLATIPVEGFVEMDITKLLALLDAPVPADVNAREAMFKTASLAARLKP
                     ELNQRPSVECVLHSLVPGRYVVHSHATAVNMLTCCLQGKTIIEELFGDDALWIPYIDP
                     GLTLARKLRDELTAWQKTTNRKSPMVIFMQNHGMIIGGDTPEEIREKTDAILAKIDKK
                     LSATCGCDIFGKVEKLPAERCRELLMCVAPALRGLLGEMNGENVNLPVVTFDDGEIAM
                     SIAAADNGSAVAAAGPLTPDQIVYCKSLPLWFASNDEESCTEVIDRLTVAMCEYKKEF
                     LFLPKVVVLQNVGIFFVGSDLAAAKTVRDVYLDAVKVMAGAKRFGAISHLSKRDREFL
                     ENWEVENYRRKISAGSSAAGRVDSKIAVVTGAAQGFGLEIAKNLAIQGASVILGDINA
                     AGSQAAADEIVAATKPGRSLGMAMNVTDATSLAAAMELIVRTYGGIDVFVSNAGVVRA
                     GSVKNQPLADFEFVTKVNYSGYFLCVQNVAPIMAWQYRTCDKWRGDIIQINSKSGLEG
                     SNRNGAYAGSKFGGIGLTQSFALELVGDGIKVNSICPGNFLDGPLWSDPEKGLFVQYL
                     KNGKVPGAKSVADVKNFYESKVPMHRGCRTADVMHAVFYLIDQKYETGQAVPVTGGQV
                     MLK"
     gene            complement(3884..5137)
                     /locus_tag="PLK08_00170"
     CDS             complement(3884..5137)
                     /locus_tag="PLK08_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51946.1"
                     /translation="MSKALDLVKNIATVDQMRQAVAAGLKPERRLKTNSHIHLPPNFS
                     AFETVEQAVTLADEQDVRVLGVSNYYDYRVYDTFAELAGRKNIFPVFGLEIISLVDKY
                     VKDGLRINDPGNPGRMYICGKGITGFASPSLRAAELLGTIRSRDEKRMAEMTAKVNAI
                     FTAAGVAVNISDADVIDMVVKRHKCPKESVTLQERHVAMAFQQKFFDVVKPAGRAAAL
                     SKIFNCQYAGKTDNDVAVQGDIRSYLMKAGKPAFVTETFLKFEEAYELILELGGIPCY
                     PTLIDGAKTLCDYERTPEELVAHLKENNIHMAELIPIRNKTDMLGRYVRAMRKADIAV
                     VGGTEHNTLDLLPLEPQCVGGVEVEDDVREIFFEGMCVAAAHQYQRLNGLAGFVNSQG
                     NRNADIKVFADAGAALIAKYFKKYC"
     gene            complement(5176..6588)
                     /gene="lpdA"
                     /locus_tag="PLK08_00175"
     CDS             complement(5176..6588)
                     /gene="lpdA"
                     /locus_tag="PLK08_00175"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPS51947.1"
                     /translation="MMSKHFNVVVIGAGPGGYVAALKAASMGAKTAIVEKHFLGGTCL
                     NYGCIPSKALLASAELLHKIERAAEFGVKVNGASFDWKAIQARKDRVLKNLRNGIAGL
                     FKARKVELFEGMGRLAGHGKVEIEGKETITADNIILAVGSIPVRIPVWPQDPNLVCTS
                     DEALHWSELPKKLLIVGGGVIGCEFACMMQSYGVDVSVVEMLPSLLPGMDEDLGAELE
                     KALAKRGIKIFTATKVESVEAAGKGVRAAISNAKGNQSLEVDKVLVAVGRRPNTKNIG
                     LDSVGMELSPKGFIQVDDTMQTAVKGIYCIGDANGRCLLAHAASAQGICAVENALGHR
                     REFHSPIPGAVYTYPEIGGVGLTESQAREKGLPISIGKFPIGFLGKAMAVNETEGFVK
                     IIRNRETDELLGMHILGHNATEIIAVGGAMLGMKATVMQLAETVFAHPTISEAVMESA
                     EDALGQALHMPPRKIIKLNV"
     gene            complement(6651..8573)
                     /locus_tag="PLK08_00180"
     CDS             complement(6651..8573)
                     /locus_tag="PLK08_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPS51948.1"
                     /translation="MQTITMPKAGKNSSGTIKKWLIAEGASFNAGDVLAVVEFEDSLG
                     EVTASSAGRLAKILAGEKKTVATGESIAQTDSAGKSAPAAKSSAPAVKPAAPLKGNPE
                     AVTVIIMPKRSNDMEEGTIVKWLVKEGDKVTKGQPIFEIETDKTSVEIEAEVDGRIAK
                     IIIGEGASAPITAPVALICDKNDDAAAWLAVNSAPAAQVEEASTAPVNIELPENVTTI
                     LMPQRSNDMEEGTIVKWLVKEGDKVTKGQPIFEIETDKTSVEIEAEVDGKLAKILLQE
                     GQSAPIKTVVALVCDNVADADKYAAGLAAQAAAEKLAVAKPAESAEKTVAAAPAQAVV
                     PTTPSVAAAPAVQRMAAQVVAGRVKASPAARRIAAQRGVNLAAISAGSGPHGRIISTD
                     VPAAPVAAATVTGKPLPAAAPVVTGPVGEVTVTTMKGMRKAIATNLTKSKQNIPHFYL
                     RFTVDAGPMLSFYKSQKAMFKCSVNDVVLMAVGRAMHEFPALRSRLEGDNVVEYPTAC
                     IGIAVGLEGGLVVPVLKDADRMTFAEIAAASRDLATQARNGKVVNMGKGSFTISNMGM
                     FGVEEFGAIINPPESGILAVGAAREGVIVENGAMRIGKIMTATISCDHRIVDGMLVAQ
                     FAGRLKELLENPQVML"
     gene            8755..9549
                     /locus_tag="PLK08_00185"
     CDS             8755..9549
                     /locus_tag="PLK08_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPS51949.1"
                     /translation="MTGQQRRELILSEVYENGRVMIKTMADRLCVSEATVRRDMKSLA
                     TDGKLELIFGGATLPNRSDFSFHSKEMRNVEAKRTVGRLAAEFVHDGEVLFLDSGTTC
                     FQMIPFLQNKRNVTIIANSARLALEFENTNVSVIMLGGQYRPDRMDTVGPMATAALDQ
                     LRGFKAFLGADGLSMDFGMTAGDIESAHIYRLALRNARQRILLVDYSKFLSPSLFKIE
                     SFEVIDRVITDRQPPEEWIEYFTRKEIDLAWPDMEDNSNTLENNDA"
     gene            9542..12016
                     /locus_tag="PLK08_00190"
     CDS             9542..12016
                     /locus_tag="PLK08_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPS51950.1"
                     /translation="MPKSQFVDPKEVRKAGQIDFTPIPVNQYNRTVKQELKSGKFTKE
                     DLIRIQRDMMLLRGFENMLNEIKTKGSYAGLEYNHRGPAHLSIGQESAAVGQSYLLGV
                     DDHTYGSHRSHSEILAKSLSAIQKLGDAELLNIMKNYFDGECLKVVEKDATGDTKALA
                     IDYLVYGALAEIFGRKAGFNKGMGGSMHTFYPPFGLMPNNAIVGGSGDISVGAALYKK
                     INKKPGIVIGNIGDASLGCGPVWEGLCLATMDQYKTLWDEAHRGGLPFILNFFNNFYG
                     MGGQTCGETMGYKILARLGAGINPEQMHAERVDGFNPLAVIDAIERKKEILARGEGPV
                     LMDTITYRFSGHSPSDAMSYRTREEVEAWQEVDPIKTFAAQLIEAGVCKQDDIDNLQQ
                     WVESVLVKAMAKAIDLNISPRTDLFKEDNLLQKTMLSNRKIENFDTSRKPETLLPFGE
                     DPRSIQIKGRSRSGFDAEGKALPKNKCVVIRDALFEAARDAFYRDSTLVAYGEENRDW
                     GGAFGVYRGLTEAIPYHRLFNSPISEGAICGSACGYALEGGRAMVELMYCDFLGRAGD
                     EIFNQLSKWQSMSGGLLQMPVVVRVSVGSKYGAQHSQDWAALVAHIPGLKVVFPATPY
                     DAKGMLNTALVGTDPVIFFESQRLYDMPEHFEKEVPAGYYEVPFGEPAIRREGKDITI
                     LTIGATLYRALDTAKILEEKYGISAEVIDARSIVPFNYEKVIESVKKTRKILLASDAC
                     ERGSFLHTMASRISQIAFDELDAPAAVVGAKNWITPPDEIEQSFFPFPNDILDAVNEY
                     LIPLAGYKTVRPCDAAKLLKDAKEGI"
     gene            complement(12496..13521)
                     /gene="amrS"
                     /locus_tag="PLK08_00195"
     CDS             complement(12496..13521)
                     /gene="amrS"
                     /locus_tag="PLK08_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPS51951.1"
                     /translation="MTQSAPKPAKWWKASGDTIECLLCPRNCHIAHGREGYCHTRKNI
                     DGKLFQMTYGLCSALAVDPVEKKPLYHFKPGSSVMSMGTVGCNLGCLFCQNCDISRAR
                     VGDIRMTPISPQDIVDAAVSHKCSSVAVTYNEPIVFAEFAMDIADAAHAANLSAVAVS
                     NGYITPLARNEFFTHFDAINVDIKAFTDTFYMELCDAHLTPILDNIEYLVHKTDALVE
                     LTTLLIPGANDSQDEVRSCAKWIYRQCGPFTPWHLTAFRPCYKLIDRPPTSLETLTRS
                     REIALEEGLKFVYTGNAVDLQGSTTFCPSCGKPVIRRDIFQVTMQAGSGMICKFCGCD
                     LSEYFIL"
     gene            complement(13518..14864)
                     /gene="amrA"
                     /locus_tag="PLK08_00200"
     CDS             complement(13518..14864)
                     /gene="amrA"
                     /locus_tag="PLK08_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPS51952.1"
                     /translation="MADNGQIIIAGLYPHPPIAVPGIGRGREEKCAATMAAMSILSQK
                     IVAAKPQCLVIIAPHSPRDRVAFGIWRGERLAGSLIHFGCPSAAVDLPNNEDFNKLLV
                     KAVKNYGLQSWRVSGDFSEDHSAIVPLWYLVKAGWQGPVSVISLNYPGSGGLDELGLA
                     IAAAAKEYGHPVAFIASGDMSHRLLRNAPAGYHPQAKMFDSEFVELLNRGDYEHVKSI
                     DADLRNLAAEDVVDSTLVAMSATGYRTRGRDVLSYEGPFGVGYTVAVLYDANGEVKEK
                     PYSLDMLLDYARAVVTAEVKGREAPKSPPPMDELARKAGVFVTIYESNGNLRGCMGTI
                     GPTEPNVVRQTEYCAKMAACEDPRFYPIRPEELSGLKFEVSILHEPEPADSEDDLDPK
                     IYGVIISTADGRRALMLPGIEQLDTVEKQLLATRQKAGIGPTEPVTIMRFKVDKIK"
     gene            complement(15038..15484)
                     /locus_tag="PLK08_00205"
     CDS             complement(15038..15484)
                     /locus_tag="PLK08_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51953.1"
                     /translation="MSIFKVIMLTTLFATFVTLAGCEKKDMKLQQPNWDWAKPKPVEK
                     EDETQTMEFAQLKKDNKKLKEDVENLTSLNAILDGRVKELLAREDRLTQELNHYKLDN
                     YKLREQVKVLGDVPAQRDRYKLQLDSAQKKIVELQDQLEREKKKSN"
     gene            complement(16254..16913)
                     /gene="trmB"
                     /locus_tag="PLK08_00210"
     CDS             complement(16254..16913)
                     /gene="trmB"
                     /locus_tag="PLK08_00210"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPS51954.1"
                     /translation="MRTNSPKTLLRDRNKKWEYDGVLLPDVSQAEVFDISEFFADPAV
                     PLEIEIGVGKGTFLVARAAYRLEVNFLGIEYAAPYAKYAADRIRRAGLTNAKMLHADA
                     ERFFKKLLPDNSVFRVHLYFPDPWPKRRHHRRRILQPEFLQHVRRVLKPGGQFLIVTD
                     HREYFEHMLKVITDAEGFARTDFPKLLDSDVHIVGTNFEKKYVEQGRNFYKAALLKYV
                     R"
     gene            complement(17020..17415)
                     /locus_tag="PLK08_00215"
     CDS             complement(17020..17415)
                     /locus_tag="PLK08_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51955.1"
                     /translation="MTGCVDEQREWVEDLYLTQSAVEHGQSVNSAQLEKRLGKPDAVL
                     SLRQFSDAVPQNQPRRKVIINDVQKCAKKHDDKVVWVYDESGRYSKPLPFPVLFGQDT
                     EFHAAIFTILRNDEVSGGFIVENWKGLKK"
     gene            complement(17478..17630)
                     /locus_tag="PLK08_00220"
     CDS             complement(17478..17630)
                     /locus_tag="PLK08_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51956.1"
                     /translation="MTADMKTIGLLCLIAGAILFVRQLVRLKKGKTTKCDCGDIFPSK
                     KDDGKK"
     gene            complement(17627..>18701)
                     /locus_tag="PLK08_00225"
     CDS             complement(17627..>18701)
                     /locus_tag="PLK08_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51957.1"
                     /translation="VAATALASRGQFAAVKPLLEHLVKSGMTDVSTFASMTLLAHGDN
                     AQVQYLRGILLDKQTQPELIISMLNQIRREKILAAMPVAQYAANSYDMLPVQIRGFMA
                     IDAMAPNAADLLCDAIKLSRNLSLSINIMRMLASRDDSTKQLTELSSRNDIVGDMARF
                     ELARRSNASSAGVYLQKLLSLGHPIIVEYVYNRIESDLADKRNVDFYIAPLANYIQSR
                     ELSSTRITPMHTLLATGISVLGDMQRPAADKVLAEIFSRPDNDPLKQLAIVALYATPN
                     RNVAAMAKPCLKSPYPDTKAYAAMLLAKFNDPAAIPALLEVQLARPTTEPDVMALVDW
                     YLLRFAGNAKTTQAVETITRSLR"
     assembly_gap    18702..18711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18712..19086)
                     /locus_tag="PLK08_00230"
     CDS             complement(<18712..19086)
                     /locus_tag="PLK08_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS51958.1"
                     /translation="MKQFKLQFFVALAAMVTTSFITVFGQVPQVSCDEAVKSLQAGLN
                     DRKEMALLLDRLSTTEDISLLPVFTKLMRNEDDRVRLLATGLVSRLAGRQAVPLLRDR
                     LFHDPAMRVRGEALSQLLKMSEL"
     gene            complement(19191..20204)
                     /locus_tag="PLK08_00235"
     CDS             complement(19191..20204)
                     /locus_tag="PLK08_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPS51959.1"
                     /translation="MSETSNDIKIIERFDELRKKIFVELDKVIVGQSQATELLLTSML
                     CGGHCLLMGVPGLAKTLMISTLARVMELSFSRIQFTPDLMPGDITGTDVIEEDRSTGR
                     REFRFVRGPVFANIVLADEINRTPPKTQAALLQAMQEHHVTAGGTTHELPKPFFVLAT
                     QNPIEQEGTYPLPEAQLDRFMFMVKVDYPSLEEERKIISVTTSRDVQDVSPVISAGDI
                     VELQGVLRRIPVSEHVVNYAVKLVRATRPADETAPQFIRDYLSWGAGPRAGQYLVLAA
                     KARALLAGRYAVDCDDIRSAAAAVLRHRLMMNFSGEAEGLNTDAIIQMLVKDTPEPGP
                     EDY"
     gene            complement(20212..21327)
                     /gene="proB"
                     /locus_tag="PLK08_00240"
     CDS             complement(20212..21327)
                     /gene="proB"
                     /locus_tag="PLK08_00240"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPS51960.1"
                     /translation="MNPTPPSQLLRQARRFVVKIGTNTLCGKDHRPDREFIDSLAGQI
                     AELNSRGIGVSLVVSGAIGTGMADMGLDSRPIGVSHLQALAAIGQGQLMRIFHDVFAK
                     HSMRVAQILVTRDGFEDRTRYLNIRNTLLTLNDYNAVAIINENDTVAVDEIRFGENDV
                     LAAHVANLLAADVLVLMTNVAGVMKAGRIIDVIQKVDEETMSLAETTRSSHGTGGMVT
                     KLTAAGMVARAGGLAVIAAGRTENILLRLADGEHIGTVFVPAKKRIGSKYLWIGQAAR
                     LAGRIIVDDGAVKALRERGKSLLPSGIVDVAGNFGKGATVVVTDTRGNRVARGLCNYD
                     AESLTRIKGLKTSEIATVLGDKPYDEAIHRDNMIVYK"
BASE COUNT         4847 a         5865 c         5539 g         5172 t
ORIGIN      
        1 agcatattcg atagctccgg gtaaaccaca atcttgccgg ccatcgtccg attcttgacc
       61 gcctcaatgc cgtcaatcgc acctgcgata ccagataccg catccagcga acagcctgtg
      121 tcaagcaggc cggattccat cttgtcgcgg acgattatca tatcgccgat gagcgatccg
      181 ctcgtgccga acatgaaaag acctttgctg atatatgcgt tgaggtcgag cacctgcttg
      241 accggcgccg gaatgccggc gaagatgttg attaatgcac ctggcttggc gtgaacaatc
      301 gcgtcagcca ccaatgcgcc gacaggagcc atgatgctat aataatcgaa tccggcttct
      361 acgggagttt tgccggtatt aacggcttca aattcgcagc cgtttttcgc ggcaagcggg
      421 ttagccttcg cagacagcgc cgccagccgg ccgtcgtcga agtccgtgcc gacgactctt
      481 acattgaatc cgagacacaa cgcacgaata acgtgcatct ggcccatagg gccgccggcg
      541 cctatgacta tcacggaatt gcccgctttc acttcgccgt tagccggaat tattttatag
      601 ctgtcagcgg cgttgtcgcc agccgtgcca atccaccgcg tcaaaccgta atgcaaacgg
      661 ccgacgccga cctcgacctc gcggtctatt ttcccgccgc cgagaactat attgataatg
      721 ccgttacgcg ccagggcatc gttgagtttt tctatcgtct cagccttcgt gccgaaataa
      781 acgatatcgt catatgcctc actggccaac tcgccacagc agcacaacgg attgacactc
      841 aagccttcga cggctgatac gccgattgca tctatcatag ccggcttgcc agcagtcatc
      901 gcttcggcaa ggcctttgac cttcctgccg gcatcgacga caacgagcat cctgccgccg
      961 gctttggggg tctggcgttc cacgtttaca tatgagcatt ccacacaggc ccacggctcg
     1021 acaagagcgg ctgcggcggc attgcattcg ctgtcgacag gaatcaggta tttatcgccc
     1081 gtgcctggct cgatgactat gcgttcgtcg aacaatatat attcctgaag agcaccttcg
     1141 aagttgtacc cgaacgctcc attcgaagca gccgtcaaaa tcttccgcca gtcggactgc
     1201 acgatgcagc ggtcgccgac cttgtgtttc ttgactttgc tgccgatttc aattattcgg
     1261 catacacact catggcctgg aactgtcggc ttgtcgccag gctgatacgc cggatattca
     1321 tcgagaatag ccccgtcaat gccgctcacg atttcaccct tgcgggcatg ttcggaaaac
     1381 tggtgcagca gtttcaggtc ggaaaaacac agacccattg cttcgacctt cgccagtatc
     1441 tggtggtcgg tgggcttgta aacgggcttt tcacgattaa gtctgagttg gtccggcccg
     1501 acgagctgga cggcacattg cgttgcagga atttttgaca ttgtttgctc catgattgat
     1561 acagttttta tccacactct tttaaaccgt tttcagacct cttatttgtc atattcatca
     1621 aggccgttct tttaaaaaaa tgcggtctcg ccatgaaaga ggcgttataa aacggttttc
     1681 agtattttat tatttcagca ttacctggcc gccggtaacc ggtacagcct ggccggtttc
     1741 atatttctgg tctataaggt agaacaccgc gtgcattacg tccgcagtgc ggcagccgcg
     1801 atgcatcggc actttggatt cgtaaaagtt ttttacgtcg gcgacgcttt ttgcgccagg
     1861 aactttgccg tttttgagat attgcacaaa gaggcctttt tccggatcgc tccacagcgg
     1921 gccgtccaaa aagtttccag ggcagatact gttgaccttt atgccgtccc cgacaagttc
     1981 cagcgcgaac gactgcgtca ggcctatgcc gccgaattta ctgccggcat aagctccgtt
     2041 gcgattgctg ccttccaggc cggacttgga atttatctga atgatatcgc cgcgccattt
     2101 gtcgcacgtc ctgtactgcc atgccattat cggtgcgaca ttctgcacgc acagaaaata
     2161 gccgctgtag ttcaccttcg taacgaattc aaaatcagcc agcggctgat tcttgacgct
     2221 gccggcacgc actacgccgg cgttcgagac aaatacgtct attccgccgt atgtccgaac
     2281 gatgagttcc atcgccgccg ccagtgatgt cgcatccgta acgttcatgg ccataccaag
     2341 cgagcggcca ggcttcgtcg cggctacgat ttcatccgcg gcagcctgtg aacctgcggc
     2401 attgatatcg cccagaataa cgcttgcgcc ctgtatggca agatttttgg caatttcaag
     2461 gccaaaaccc tgtgccgcac ctgtaacgac cgcaattttg gaatcgactc tgccggcggc
     2521 tgaactgccg gcggaaatct tgcgccgata attctcgact tcccagtttt cgagaaactc
     2581 gcggtcacgt ttcgagaggt ggcttattgc gccgaaccgt ttcgcgccag ccatcacctt
     2641 tacggcatca agatagacat cacggacggt cttcgccgcg gcaagatcgc tgccgacgaa
     2701 aaatatgccg acgttctgaa gcactacaac cttcggcagg aataaaaatt cctttttata
     2761 ttcgcacatc gccacggtca gcctgtcgat tacttcggtg cacgattctt catcattcga
     2821 cgcgaaccac agcggaagcg acttacaata cactatctga tccggcgtaa gagggccagc
     2881 cgccgcaacg gctgaaccgt tgtccgccgc cgcaatgctc atcgcgattt cgccgtcgtc
     2941 aaaagtcaca accggcagat tgacgttttc tccgttcatt tcgccgagca gtccgcgaag
     3001 agcaggagca acgcacatca gcaattccct gcaacgctca gccggcagct tttcaacttt
     3061 tccaaaaata tcacagccac acgtagctga aagtttttta tctattttcg caagaatcgc
     3121 gtcggttttt tcccgtattt cttccggcgt atcgccgccg attatcatgc cgtgattctg
     3181 catgaaaatt accatcggcg atttacgatt cgtcgttttc tgccatgcag tcagttcgtc
     3241 gcgtaatttg cgggctaacg tcaggcccgg gtctatgtac ggaatccaga gagcatcgtc
     3301 gccgaaaagt tcttctatta tcgttttgcc ctgaaggcag catgtgagca tgttgacggc
     3361 ggtggcgtgc gagtgaacca catatctgcc aggcactagc gaatgaagca cgcactccac
     3421 gctgggccgc tggttaagtt caggtttcag acgggcagcc agcgacgcag tcttgaacat
     3481 ggcttcgcgt gcgttgacat ccgccggcac aggcgcgtcg agaagggcta acagctttgt
     3541 aatgtccatc tccacgaacc cctcaaccgg tatcgtcgcc agtgaatggc ctgaaccttt
     3601 cacatacaac tttccgtcta tcttgcccga cgtattgccg ccgccggcaa gcactatcgc
     3661 agggtcactt ccgtaaaagt gcgacagttt cacgagtgcg cctattgccg tttcgccttt
     3721 gaaatcaggc cattcaatct ttgccatata ttttaatctc ctcgtcgccg caatcatcgc
     3781 ggacggctga tattactaac agtacttttt gagatatttt gcagttatct ctgatattgc
     3841 taacagtatt ttttaaaata ttttgtaatc agcgatgata ttactaacag tactttttaa
     3901 aatattttgc aatcagcgcg gcacctgcat cggcaaacac ttttatgtct gcgttgcggt
     3961 taccctgcga attaacaaat cccgccaggc cgttaagcct ctgatactga tgtgccgcag
     4021 ctacgcacat gccttcaaag aaaatttccc tgacgtcgtc ctcgacttcc acgccgccga
     4081 cacactgcgg ctcaagcggc agcaggtcga gcgtgttatg ctccgttccg ccgactacag
     4141 cgatatcggc tttccgcatg gcgcggacgt atctgccgag catgtcggtc ttgttgcgaa
     4201 tcggaatcag ttccgccatg tggatattgt tttccttgag gtgggcaaca agttcttctg
     4261 gcgtacgttc ataatcacag agcgtttttg ccccgtcgat gagcgttggg tagcacggaa
     4321 tgccgccgag ttcgaggatg agttcatatg cttcttcaaa tttgaggaat gtttcggtaa
     4381 cgaaggcggg tttgccggct ttcatcagat atgagcggat gtcgccctgt acggcgacgt
     4441 cgttatcggt tttgccggca tactggcagt taaaaatttt tgacagcgcg gcagcgcggc
     4501 cggcaggttt aaccacgtca aaaaatttct gctggaacgc catcgcaacg tgtcgttcct
     4561 ggagcgtcac cgattccttc gggcacttgt gacgttttac gaccatgtcg attacatcgg
     4621 catcgctgat gttcacggct acgccggcag cggtgaaaat ggcattaact ttcgccgtca
     4681 tttcagccat gcgtttttcg tcacggctgc ggattgtgcc cagcagttca gccgcccgca
     4741 acgacggcga cgcaaaaccc gtaatgcctt tgccgcagat gtacatgcgg ccaggattgc
     4801 caggatcatt tatacgcaag ccgtccttga cgtatttgtc gacaagcgaa attatctcaa
     4861 gcccgaaaac cggaaaaata ttcttccggc cggcgagttc cgcaaaagta tcataaacgc
     4921 gataatcata ataattgctg acgccaagaa cgcgaacgtc ctgttcgtcg gcaagcgtga
     4981 cagcctgctc gacagtttca aacgcggaga aattcggcgg cagatgaatg tgcgaattcg
     5041 tctttagccg gcgttccggt ttaaggcctg ccgcgacggc ttgacgcatc tggtcaaccg
     5101 tggcaatgtt tttgacgagg tcgagtgctt tgctcataaa attcctcaag aaacgtgaga
     5161 gtgattttac acatttcaca cattcaattt gattattttc ctcggcggca tatgcagtgc
     5221 ctggccgaga gcgtcttcgg cggattccat aaccgcttcg cttatggtcg ggtgtgcaaa
     5281 caccgtctca gccagttgca taacggtagc tttcatgccg agcatcgcac cgccgacggc
     5341 tatgatttcc gtggcattgt ggccgaggat gtgcatgcca agcaactcgt ccgtttcgcg
     5401 gttgcggatg attttgacaa aaccctctgt ttcatttaca gccatcgcct tgccgaggaa
     5461 gcctatcggg aacttgccta tcgatatcgg caggcctttt tcgcgagcct gcgactctgt
     5521 cagccctacg ccgccgattt ccggatacgt gtaaaccgcg cctggaatcg gcgaatggaa
     5581 ctcgcgtctg tggccgagcg cgttttcgac cgcgcatatg ccctgggcac tggctgcgtg
     5641 cgccaacaga caacggccat tggcgtcgcc gatacagtaa atgcctttga ccgcagtctg
     5701 catggtatcg tcgacctgga taaatccttt gggtgaaagt tccatgccta cgctgtcaag
     5761 gccgatattc ttggtattgg gccgacggcc tactgccaca aggactttgt caacttcaag
     5821 cgactgattg ccttttgcat tggatatggc tgccctgacg cctttgccgg cggcttccac
     5881 tgattccact ttcgtagctg tgaaaatctt tatgccgcgt ttcgcgagag ctttttcgag
     5941 ctccgcgcca aggtcttcgt ccatgcccgg cagcaggctc ggcagcattt caacaacact
     6001 cacgtcgacg ccgtaactct gcatcatgca cgcaaactcg cagccgatga caccgccgcc
     6061 aacgattagc agtttcttgg gcagttcgct ccagtgcaag gcctcgtcgg acgtgcagac
     6121 aaggttcgga tcctgcggcc agaccggaat gcgaaccgga atcgagccga ccgccaggat
     6181 tatattatcg gcagtaatgg tttccttgcc ttcgatttca accttgccat ggccggcgag
     6241 tctgcccata ccttcgaaca gttcgacctt gcgagccttg aaaagaccgg caatgccatt
     6301 gcgaagattt ttgagtaccc tgtccttacg agcctggata gccttccagt caaacgacgc
     6361 cccgttgacc tttacgccaa attcagcggc acgttcgatt ttatgcagca actcagccga
     6421 cgcaagcagc gctttggacg gaatgcagcc gtaattcaga cacgtcccgc cgagaaaatg
     6481 cttttcgacg attgctgttt ttgcacccat cgatgcggct tttaacgcgg caacgtatcc
     6541 gccagggccg gcaccgataa cgacaacatt gaaatgcttt gacatcatta ttcctcaaat
     6601 aaattcgaaa tacgaatccc gaaattcgaa acaaattcgg atttcagatt ttacaacatc
     6661 acctgcgggt tttcgagcag ttctttcagg cggccggcga actgggccac aagcatgccg
     6721 tcgacaattc tgtggtcgca gcttatcgtc gcggtcatga ttttgccgat tcgcattgcg
     6781 ccgttttcga cgataacgcc ttcgcgtgcc gcgccgactg ccaaaatgcc gctttccggc
     6841 ggattgataa tcgcgccgaa ttcctcaacg ccgaacatgc ccatgttcga aatggtgaac
     6901 gagcccttgc ccatattgac gaccttgccg ttgcgagcct gcgtcgccag atcacggctg
     6961 gcggcggcta tttcagcaaa ggtcatgcgg tctgcatcct tgagcaccgg cacaaccagg
     7021 ccgccttcca ggccgaccgc tatgcctatg caggctgtcg gatactcgac cacgttatcg
     7081 ccctcaagcc gcgaccgcaa tgccggaaat tcatgcatcg ccctgccgac agccatcaac
     7141 acaacgtcgt tcacactgca cttgaacatc gccttctgcg atttgtagaa cgaaagcatc
     7201 gggccggcat cgacggtaaa acgcagataa aagtgcggaa tgttctgctt ggatttcgtg
     7261 agattcgtgg cgatagcctt tcgcatgcct ttcatcgtcg tgacggtgac ttcgccgacg
     7321 ggacccgtaa cgacaggtgc ggcagccggc aacggcttgc cggttacagt cgcggcggca
     7381 accggtgcag ccggaacgtc ggtcgaaatg attcggccat gcgggcctga gccggctgat
     7441 atggcagcta agtttacgcc tcgctgggct gcaattctgc gagccgcagg cgatgccttc
     7501 actctgcctg cgacaacctg ggcagccatg cgctgtactg ccggcgccgc agcaacagaa
     7561 ggtgttgtcg ggacaactgc ctgagccggt gcagcagcga cagttttttc tgcagattct
     7621 gccggttttg caactgcaag tttttccgcc gcagcctgcg cagccaagcc ggcggcatat
     7681 ttgtcagcat cggcaacgtt atcgcaaaca agcgctacga cggttttaat cggcgccgat
     7741 tggccttcct gcaaaagtat ttttgcgagt ttgccgtcga cttccgcctc tatctcaacg
     7801 gaagtcttgt ccgtctctat ttcaaatatc ggctggcctt tggtgacctt gtcgccttcc
     7861 ttcaccagcc acttgacgat tgtaccctct tccatatcat tgctgcgttg cggcatcaga
     7921 attgtcgtga cgttttcggg cagttcaatg ttcactggtg ctgtggaagc ttcttcaact
     7981 tgagcggctg gggcggaatt caccgccagc catgccgccg catcgtcatt cttatcgcaa
     8041 atgagtgcaa ccggtgcggt tatcggggct gacgcgcctt cgccgatgat tatttttgca
     8101 attctgccgt cgacttccgc ctctatttca acggaagtct tgtcggtttc gatttcaaat
     8161 atcggctggc ctttggtgac cttgtcgcct tccttaacca accacttgac gatagtgccc
     8221 tcttccatgt cgttgctgcg tttgggcatt attatgactg taaccgcctc aggattgccc
     8281 ttgagcggcg cggcaggttt taccgccgga gcagacgact tggccgccgg tgcggatttg
     8341 cctgctgaat cagtctgggc tatcgattcg cctgttgcga cggtcttctt ttcgccggcg
     8401 agtatcttcg ccagccggcc agccgacgat gccgtcactt cgccaagcga atcttcaaat
     8461 tcaaccacgg cgagcacgtc gccggcatta aaggacgcac cttcggcaat tagccacttt
     8521 tttatcgttc cagaggagtt cttgcccgct ttgggcatgg tgatggtctg catttcaata
     8581 cctcatatgt tacagtgtgt gaaaactttt tcgcaataac ctgagtctaa attattccaa
     8641 ctcgatgaaa tagtcaagca tgatattgca aaactatgac aaaaaattga aatttttttt
     8701 cagaattttg ttgatttttt gcgtgaatcg tagtttgata gactcgatat gagtatgacc
     8761 ggccaacagc gacgagaact aattctctcg gaagtgtatg aaaacgggag agttatgata
     8821 aaaacaatgg ctgaccgcct ttgcgtgagt gaggctacgg tcaggcgtga catgaaatcg
     8881 ttggctaccg acggcaaact tgagctgata ttcggcggcg caactctgcc gaatcgcagt
     8941 gatttttcgt tccactccaa ggagatgcgg aacgttgaag ccaaacgcac cgtcggccgg
     9001 ctggcggctg agttcgtaca cgacggcgaa gtgctttttc tggattccgg cacaacctgt
     9061 ttccagatga ttccgtttct gcagaacaag cggaatgtga cgattatcgc caattcagcc
     9121 cggctggcgc tggagttcga gaataccaac gtcagcgtga taatgctggg cgggcagtat
     9181 cggcctgaca ggatggacac cgtcggcccg atggctacgg cggcgttgga ccagcttcgc
     9241 gggttcaagg cgttcctggg cgcagacggg ctgagcatgg atttcggcat gacggctgga
     9301 gatatcgaaa gcgcgcatat atatcgcctc gcgctgcgga acgcacgcca gcggattctg
     9361 ctggtggact acagcaagtt cctcagcccg tcgcttttca aaatcgaatc gtttgaagtt
     9421 atagaccggg tcataaccga caggcagcct ccggaagagt ggatagagta tttcacccgc
     9481 aaggagatag acctggcgtg gcccgatatg gaagataaca gtaacacgtt ggagaataac
     9541 gatgcctaaa agtcaattcg tagatccaaa ggaagttcgc aaagccggcc agattgattt
     9601 caccccaatt ccggtaaatc aatacaatcg cacggtgaaa caggaactca agtccggcaa
     9661 gttcaccaag gaagacctca tccgcattca gcgcgacatg atgctgctcc gcggattcga
     9721 gaacatgttg aacgaaatca agaccaaagg ctcatacgcc ggcctggaat acaatcatcg
     9781 cggccctgcg cacctgtcca tcggccagga gtcagccgcc gtcggccaat cctatctgct
     9841 cggcgtcgac gaccacacct acggcagcca tcgcagccac agcgagattc tggccaaaag
     9901 cctctccgcc attcagaaac tcggcgatgc cgaacttttg aacataatga aaaactactt
     9961 tgacggcgaa tgcctgaaag tcgtcgaaaa agacgcaacc ggcgacacga aagcgttagc
    10021 gatagattat ctcgtttacg gcgcattggc ggaaattttc ggccgcaaag ccggcttcaa
    10081 caaaggcatg ggcggctcga tgcacacttt ttatccgccg ttcggcctta tgcccaacaa
    10141 cgccatcgtc ggcggcagcg gcgatatcag cgtcggagcc gcactttaca agaaaatcaa
    10201 taaaaagccc ggcatagtca tcggcaatat cggcgatgct tcgctcggct gcgggccggt
    10261 atgggaaggc ctctgcctgg ctactatgga ccagtacaag accctctggg atgaagccca
    10321 ccgcggcggc ctgccgttca tattgaactt tttcaacaac ttctacggca tgggcggcca
    10381 gacctgcggc gaaacgatgg gttacaaaat tctggctcgc ctgggcgcag gcataaatcc
    10441 ggaacagatg cacgcagagc gggtggacgg cttcaatccg ctggctgtca tagacgcaat
    10501 cgaacgaaag aaggaaatcc tcgcccgcgg cgaaggacca gtattgatgg acaccatcac
    10561 ctaccgcttc agcggccaca gcccgtcgga cgcaatgagc taccgtacgc gcgaagaagt
    10621 cgaagcctgg caggaagtag acccgataaa gacattcgcg gcccagctca tcgaagcggg
    10681 cgtatgcaaa caggacgata tcgacaacct gcagcagtgg gttgaaagcg tattggtaaa
    10741 ggcgatggca aaggctatcg acctgaacat atcgccccgc accgacctgt tcaaggaaga
    10801 caacctgctg cagaagacga tgttatccaa caggaagatt gaaaatttcg acacgtcgcg
    10861 caagcctgaa accctgctgc cctttggcga agacccacgc agcatccaga ttaaaggccg
    10921 cagccgttcc ggcttcgatg cggaaggcaa ggcactgccg aaaaacaaat gcgtcgtcat
    10981 tcgcgacgcc ctcttcgagg cggctcgcga cgcattttat cgcgactcga ccctggttgc
    11041 ctacggcgaa gaaaaccgcg actggggcgg tgcgttcggc gtatatcgcg gcttgacgga
    11101 agccattccg tatcacaggc tgttcaattc gccaatcagc gaaggcgcaa tctgcggcag
    11161 tgcctgcgga tacgccctcg aaggcggccg cgcaatggtg gaactgatgt actgcgactt
    11221 cctcggccgc gccggcgatg aaatattcaa tcagctctcc aaatggcaga gcatgtccgg
    11281 cggcctcctt cagatgccag tcgtggttcg agtatcagtc ggcagcaaat acggcgcaca
    11341 gcacagccag gattgggcgg cacttgtggc gcatattccg ggcctgaaag tcgtatttcc
    11401 cgcaacccca tatgacgcca aaggcatgct gaacacggct ctggtcggaa cggacccggt
    11461 catattcttc gaatcgcagc ggctgtatga catgccggaa cattttgaga aagaagtgcc
    11521 ggcaggttac tatgaagtac cattcggtga gccggcaatc cgccgtgaag gcaaagacat
    11581 tacaatccta accatcggcg cgacactcta cagggcattg gacacggcga agattctgga
    11641 agaaaaatac ggcatcagtg cggaagtgat agacgcccgc tcaatagtgc cgttcaacta
    11701 tgaaaaggtc atcgagtcgg tcaagaaaac ccgcaagata ctgctagcca gcgatgcgtg
    11761 tgaacgcggc agcttcctgc acacgatggc cagccgaatt tcgcagattg cgttcgatga
    11821 actcgacgct ccggctgcag tcgtaggcgc aaagaactgg attacgccgc cagacgaaat
    11881 cgagcagtcg ttcttcccgt tcccgaacga tatcctcgat gccgtcaacg aatacctgat
    11941 tccgttagcc ggttacaaaa ctgtccggcc atgcgacgca gccaagctgc tgaaagacgc
    12001 aaaagaaggt atatgaaaaa tttcaaattt ggaaattgca gattgttaga gcattgaaag
    12061 attgagagtt catatttttg cgccagtggg tttgttcgtg gcaaggaagg aaaatgaggc
    12121 atacttggca gtattctgaa gtttttctga cgcagccacg gacaaagcaa accagcaaaa
    12181 aaagaactct caagattgaa ctgctctaat tgtttttatt tttcaaactg cccgatttat
    12241 atggtttttc gcatataagg cggaaagtta aaaatattgt caatcggtcg ctttgaataa
    12301 tttctgattt gtagtttctg attttaaacg atatgcaggg acacacgccg gaataatatt
    12361 cttgcgtggc gcggcggata ccggatgtat ccgccgtgtt ttttttgcgc ggattttgaa
    12421 aacctcgaac tctgttaata aaagcccata cgccagaaca ttgcaagact gtcaagaacc
    12481 gctataaaac ttgctctaca aaataaaata ttcactcaaa tcgcagccgc agaatttgca
    12541 tatcatgcca ctgccagcct gcatggtcac ttgaaaaatg tcgcgacgga taaccggctt
    12601 gccgcatgat ggacagaacg tggtcgaacc ctgtaaatcg accgcattgc cggtatagac
    12661 gaatttcaag ccttcttcca gtgctatttc gcgagaccgc gttagcgttt ccagcgatgt
    12721 cggcggccgg tcaatcagtt tgtagcatgg cctgaacgca gtcagatgcc acggcgtaaa
    12781 cggcccgcat tgccgataaa tccatttggc gcaactgcga acttcatcct gtgaatcatt
    12841 cgcaccaggt atcagaagcg tcgtaagctc aaccaatgca tcagtcttgt gcacgagata
    12901 ttctatattg tccagaatcg gcgtaagatg tgcgtcgcag agttccatat aaaaagtgtc
    12961 ggtgaaggct ttgatgtcca cgtttatggc gtcaaaatgc gtgaaaaatt cattcctggc
    13021 caacggcgtg atataaccgt tcgacactgc caccgccgaa aggttcgccg cgtgagccgc
    13081 atcggcgatg tccatcgcga attccgcaaa aactatcggt tcgttgtacg tcacggcgac
    13141 cgagctgcat ttatggctga cggcagcgtc gacgatgtcc tgcggagaaa tcggcgtcat
    13201 tcgtatgtcg ccgacccgcg cacgcgaaat gtcgcaattc tgacagaaca ggcagccgag
    13261 gttgcatccc accgtcccca tcgacatgac actactgcct ggtttgaaat ggtatagcgg
    13321 ttttttctcc accgggtcaa cagccagcgc actgcacaaa ccgtaagtca tctggaacag
    13381 tttgccgtcg atattttttc gcgtatgaca atagccttca cggccatgcg caatatggca
    13441 gttacgcgga cacaggaggc attctatcgt gtcaccggat gctttccacc atttggccgg
    13501 tttgggcgca gactgcgtca ttttatcttg tccaccttga atctcattat cgtcacaggc
    13561 tcggtcgggc cgatgccggc cttctgcctt gtggctaaaa gctgtttttc aacagtgtcg
    13621 agctgttcta ttccaggcag catcagtgcg cgtctgccgt cggcggtgga gattatcacg
    13681 ccgtaaattt tggggtcaag atcatcttcc gaatctgccg gttccggctc atggagtatg
    13741 ctcacttcaa atttcaggcc gctcagttct tcggggcgta tcggatagaa acgcgggtct
    13801 tcacacgcag ccattttggc acaatattcg gtctgccgca ccacgttcgg ctcggtcggg
    13861 ccgattgttc ccatacagcc gcgcagatta ccgttcgatt cgtagatagt tacgaatacg
    13921 ccggctttgc gagccagttc atccatcggc ggcggcgatt tgggggcttc gcggcctttg
    13981 acttcagcgg ttacgacggc acgggcgtaa tccagcagca tatcaaggct gtatggtttt
    14041 tcttttactt cgccgttggc gtcgtaaaga acggccaccg tgtagccgac gccgaaaggc
    14101 ccttcgtaac ttagcacgtc gcggccgcga gtgcgatagc cggtggctga catcgccacg
    14161 agcgtagaat ccaccacgtc ttccgccgcc aaattgcgta gatcagcatc gatacttttt
    14221 acatgttcgt aatcgcccct gttcaggagt tccacgaatt cactgtcgaa cattttagcc
    14281 tgcggatgat agccggctgg cgcattgcgc aaaagccggt gactcatatc gccgctggcg
    14341 atgaatgcaa ccggatggcc gtactctttt gcggcggcag ctatggccag gccgagctca
    14401 tccaatccgc cgctgccggg gtaattcagg ctgatgacgc tcacggggcc ctgccaaccg
    14461 gcttttacca gataccacaa cggcactatc gccgaatgat cttcggaaaa atctccgctt
    14521 acgcgccacg actgcaagcc gtaattttta acggctttca caaggagttt gttgaaatct
    14581 tcattattgg gcagatccac agcagccgac ggacagccga aatgaatcag cgaaccggca
    14641 agacgttcgc cgcgccatat tccgaacgcc acgcggtcac gcggtgaatg aggggctatt
    14701 atcacgaggc attgcggctt tgcggcgacg attttttgcg aaagaatgct catggcagcc
    14761 atggtggcgg cgcatttttc ttcacggcca cggcctatgc caggcacggc aatcggcgga
    14821 tgcggatata gaccggcaat gattatctgc ccattatcag ccatcgattt ctccttctga
    14881 ctatcattat atgcggcaaa ataacccaaa aacgggaaat caggcggaat gcatcgaaga
    14941 gcaaaaatca attcccctag cacagactgc tttccgcaag aaacccaact gtcctgcaaa
    15001 caattccctg caataaattc gtgcgccaag ccggcggtca gttgctcttt tttttctctc
    15061 tttccagctg atcctgcaac tcgactattt ttttttgagc cgagtcaagc tgaagtttgt
    15121 aacggtcccg ctgcgccgga acgtctccca aaactttcac ctgctccctg agcttgtaat
    15181 tgtcaagttt gtaatggttc agttcctggg taagacggtc ttcgcgggca agaagctctt
    15241 taacgcggcc gtcaagtatc gcattaaggc tggtaagatt ttcaacgtct tccttgagtt
    15301 ttttattatc cttctttaac tgggcaaact ccatagtctg agtttcgtct tccttttcca
    15361 caggcttggg tttggcccag tcccagttcg gctgctggag cttcatgtct ttcttttcgc
    15421 agccggcaag cgttacgaag gttgcgaaaa gcgtcgttaa catgatgact ttaaaaatac
    15481 tcatgcggaa atcataacac caaacggctg cgttcgtcca gagtttttta ctttgccgtc
    15541 ggcgggtttt ccgaattttc atccatgcga aaatgagcgc aaaaaaaatg ggcgacaacg
    15601 gttgctcaga cctgttatcg cccttcgcct tgctgtagag atccttatcc gttgaaaccg
    15661 gcaggaggag aaaaaccggt atgatctgcg ggcgctcatc tccgcaaacc ctcgttcggc
    15721 ataatctcta ctctataaac gaggcctgac cgcgatacaa ctgaaaaatc aggatcgcaa
    15781 gcttccgtgt gggcgctcat ctccacatgg tgcaaataca gacctcttct agaggctcgt
    15841 gtagttccgt ttgtctgaac cggcagttgg ttggttggga gaagaaaaga gttaccagtt
    15901 cagccctctc ggctcctcga aatacaactc tcgaagtcga aaccgtccct acactaaaaa
    15961 atattagatt gccaaggcgt caaaatgcga cactttgcgc cgcacaatat caggtgtgca
    16021 cctggcatgc caaaattcgg ccgaaaacca taccgaggct aattttgtca taacatgcct
    16081 aaaacaataa cattaagaca aagaagattt ttaaaatttt ttaaacaaac cgccgcattc
    16141 cctcgaaacg ctatagcatt actgttgcag aatgcaacac gaaacttatg caggcccata
    16201 ggttcaaagc aattttagaa gtgattatcg ccgcccaaat cggaacagcc gcgctaccgg
    16261 acatacttga gaagtgccgc tttataaaaa tttctgccct gctcgacgta ttttttctca
    16321 aaattcgtgc cgacgatgtg tacgtcgcta tcgagcaatt tggggaaatc ggttcttgca
    16381 aaaccttcgg cgtcggtaat aaccttcagc atatgttcga aatattcacg atggtcggtg
    16441 actattaaaa attgtccgcc aggcttgagc actcgccgga catgttgcaa aaattccggc
    16501 tgaagtatgc gacggcgatg atgtctgcgt ttgggccacg ggtcagggaa gtacagatgc
    16561 acacgaaata cgctgttgtc gggcagcagt tttttaaaaa aacgctcagc gtcggcgtgg
    16621 agcatttttg cgttggtcag gcctgcgcgg cggatgcggt cggcggcgta tttggcatac
    16681 ggcgcggcgt attcgatacc caggaaattg acctccagcc gatatgctgc ccgtgcgacg
    16741 agaaatgtgc ccttgcccac accgatttca atctccagcg gcactgcagg gtctgcaaaa
    16801 aattccgata tgtcaaaaac ctcagcctgc gaaacatccg gcaggagtac gccgtcgtac
    16861 tcccattttt tgttcctgtc gcgtaaaagc gttttggggg aattcgttct catggccgag
    16921 gattgtagta agatgacaat aaaatttcaa tttgattcaa ttcaacagcc gactttcaag
    16981 tatcgaataa tgattaaatt ccatcaaaag agacggtttt tactttttga gacctttcca
    17041 gttttcgacg atgaaaccgc cgctgacttc atcgttccgg agaatggtaa agatggcggc
    17101 atggaattcg gtatcctggc cgaagagcac aggaaacggc aatggcttgg aataacggcc
    17161 ggattcgtcg tatacccaga cgactttatc gtcgtgtttt tttgcacatt tctgcacatc
    17221 gtttattatc actttgcggc gcggctgatt ttgcgggacg gcatcgctga actgccggag
    17281 cgacaatacg gcatccggtt tgccgaggcg tttttcgagt tgtgccgagt tgacggactg
    17341 gccgtgttcg acggctgatt gcgtgaggta gagatcctcc acccactccc gctgttcgtc
    17401 gacacagccg gtcataatgc atccgcatac aatcgccgtc gtaacaatcg ttttaaattt
    17461 taaaaaattc ggcagattca tttcttaccg tcgtcttttt ttgatggaaa tatatcgccg
    17521 cagtcgcatt tggtggtttt gccttttttt aaccttacaa gctggcgaac gaaaagtatt
    17581 gcgccggcga taaggcaaag aaggcctatt gttttcatgt cagccgtcat cgcaggctcc
    17641 tggtgattgt ttcaaccgcc tgcgtggttt tggcattgcc cgcgaatcgc agcagatacc
    17701 agtcaacgag ggccatcaca tccggctcgg ttgtcgggcg ggcgagttgc acttcgagca
    17761 acgccggtat ggcagccggg tcgttgaact tcgccagcag catggctgca taagccttgg
    17821 tgtccggata cggacttttg agacatggct tggccatggc ggcgacgttt ctattcggag
    17881 tcgcataaag cgcaacgatg gcaagctgtt tgagcgggtc gttgtcggga cggctgaata
    17941 tttcagccag caccttgtcg gcagccggcc gttgcatgtc gccgagcacg cttatgccgg
    18001 tagccaacag cgtgtgcatc ggcgttatgc gggtgctcga aagctcacgc gactgtatat
    18061 agttcgccag cggcgcgatg taaaaatcga cgttgcgttt gtcagccagg tcgctttcga
    18121 tacggttgta tacatactcg actataatcg gatggcccaa actcagcagt ttctgcagat
    18181 aaacgccggc actcgacgca ttgctcctgc gagccagctc gaatctcgcc atatcgccga
    18241 cgatgtcatt tcggctggac agctcggtga gctgtttggt cgaatcgtcg cgcgacgcca
    18301 gcattcgcat gatgttgatg ctgaggctga gattccggct gagtttgatt gcgtcgcaga
    18361 gcaggtcggc tgcgttcggg gccatggcat cgatagccat gaatccgcgt atctgcaccg
    18421 gcagcatgtc gtaggaattc gctgcgtatt gagccaccgg catggcagcc aggatttttt
    18481 cccgccgaat ctggttgagc atgctgatga tgagttccgg ctgggtctgc ttgtccagca
    18541 gtatgccgcg cagatactga acctgcgcat tgtcgccgtg agccagcaac gtcatggatg
    18601 cgaacgtgga tacgtccgtc atgccggact tgacaaggtg ttcgagaagc ggcttgaccg
    18661 cggcgaactg accgcggctg gctagcgcgg tggctgcgac gnnnnnnnnn ngagttcgct
    18721 cattttcaag agttggctga gcgcttcgcc gcggacgcgc atcgccgggt cgtggaagag
    18781 tctatcacgc aacaggggca cagcctgccg gccggcaagc ctggacacca gccccgtcgc
    18841 caacagccgt acgcggtcat cttcattccg catgagcttg gtaaaaaccg gcagcagtga
    18901 aatgtcttcc gtcgtactca gccggtcaag cagcaacgcc atctctttcc tgtcattcag
    18961 gccggcctgg agagatttga ccgcttcgtc gcatgaaacc tgcggcacct gcccgaaaac
    19021 tgtgataaaa gacgtcgtaa ccatcgctgc cagcgcaacg aagaattgta atttaaattg
    19081 cttcatattt ttcctctatt tactcattca tcgctatatt ctgttctgaa aaaacctgac
    19141 agtcagtccg ccatattatg caaaagactg ctgcaggcgt tcaataatca tcaataatct
    19201 tccggcccgg gttcgggcgt gtctttgacg agcatctgga tgattgcgtc ggtattaagg
    19261 ccttccgcct cgccagaaaa attcatcatg agtctgtgcc gcagaacggc ggcagcggcg
    19321 gagcggatat cgtcgcagtc gactgcgtat ctgccggcca ggagcgccct tgccttggcg
    19381 gctaacacga ggtattggcc ggctcgcggg cctgcgcccc atgacaggta gtccctgatg
    19441 aattgcggcg cggtttcatc cgcaggccgc gtcgcgcgaa ccagctttac cgcataattc
    19501 accacgtgct cactaaccgg aatccggcgt aatacgccct gaagttcaac tatatcaccc
    19561 gcggaaatca ccggcgaaac atcctgcaca tcgcgggaag tggttacgga aattattttg
    19621 cgttcctctt ccagcgaagg ataatcgacc ttgaccatga acatgaaacg gtcaagctgc
    19681 gcttcgggca gcgggtaggt gccttcctgt tcgatggggt tctgcgtagc gagcacgaag
    19741 aacggcttgg gcagttcgtg cgtcgtgccg cctgcggtta cgtggtgctc ctgcatagcc
    19801 tgcagaaggg cagcctgcgt ttttggcggc gtacggttga tttcgtcggc aaggacgatg
    19861 ttcgcaaaca cggggccgcg gacgaagcgg aattcccgcc ggccagtcga acggtcttcc
    19921 tctatcacgt ccgtgccggt tatgtcgccg ggcatgaggt cgggcgtgaa ctgaatgcgg
    19981 ctgaacgaca gttccatgac gcgggcgagc gtggatatca tcagcgtttt cgcaaggccc
    20041 ggcacgccca tgagcagaca gtggccgccg cagagcatgg acgtcaaaag aagttccgtc
    20101 gcctgcgatt ggccgacaat gaccttgtca agctcgacga atatcttttt gcgcagttcg
    20161 tcgaatcgct ctatgatttt gatgtcgttt gaagtttcgc tcatggtttc ctcacttgta
    20221 gacaatcatg ttatcgcgat gaatcgcctc gtcgtagggt ttgtcgccga gtacagttgc
    20281 tatttccgac gttttaaggc ctttgatgcg tgtcagcgat tcggcgtcgt aattgcaaag
    20341 gccgcgggct acgcggttgc cgcgggtatc cgtcacaact accgtagcgc ctttgccgaa
    20401 attgccggct acatcgacta tgccggacgg cagaaggctt ttgccccgtt cccgcaacgc
    20461 cttaaccgcg ccgtcgtcca cgattattct gccggcaagc ctggcagcct ggcctatcca
    20521 gagatatttg ctcccgattc gttttttcgc cggcacaaaa accgtgccga tgtgttcgcc
    20581 gtcggctaac cgcagaagga tattttccgt tcggccggct gcaatcaccg ccaggccgcc
    20641 tgcacgggca accatgccgg ctgccgtgag tttggtaacc atgccgccag tgccgtggga
    20701 acttctggtc gtttcggcca ggctcatggt ttcttcgtcg accttctgta tcacgtcgat
    20761 gattcggccg gctttcatta cgccggcaac gttggtcatg agcacaagca catccgccgc
    20821 aagcagattg gccacgtgcg ccgccagaac gtcattttcg ccaaaccgga tttcatcgac
    20881 ggcgacagta tcattctcgt tgatgatggc aaccgcgttg tagtcattca gcgtaagcag
    20941 tgtgttgcgt atgttcagat atcgcgtgcg gtcttcaaaa ccgtcgcgcg tcacgagtat
    21001 ctgcgcgact ctcatcgaat gtttggcgaa aacgtcgtga aaaatccgca tgagctggcc
    21061 ttggccaatc gcagccaatg cctgcaaatg cgatacgcct atcggccgtg aatccaggcc
    21121 catgtcggcc atgcccgtgc cgatagctcc gctgacgaca agcgacaccc ctatgccgcg
    21181 cgagtttaac tccgcaatct ggccagccag cgagtcgatg aactcgcggt ccggccgatg
    21241 gtctttcccg cacagcgtgt tcgtgccgat tttcacgaca aaccgccgtg cctgtcgaag
    21301 caactgtgat ggaggtgtag gattcatttt atcattccag ttcgtcggtg gcgatatcgc
    21361 cagccaaaac ggccggcaca cggtgaagta cgtggcagac aagctcgaca ttgcgttttg
    21421 tcgcaccctg ttg
//
LOCUS       DAONFI010000003        19766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_2823_length_19766_cov_4.895743, whole genome shotgun sequence.
ACCESSION   DAONFI010000003 DAONFI010000000
VERSION     DAONFI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19766
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2823_length_19766_cov_4.895743"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="argC"
                     /locus_tag="PLK08_00245"
     CDS             <1..871
                     /gene="argC"
                     /locus_tag="PLK08_00245"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPS51961.1"
                     /translation="AKQVKMAQQPYSPDTVAALKPDVALLCVPHQIAMSYVPQLLAKG
                     VKIIDWSADYRIRNAAVYEKWYCEHTDKTSLAHAVYGLPELYRNQIKTAKLVANPGCY
                     PTCSILALAPLLRAGLIESENIFISAVSGVTGAGRKATLENHYPERNENFEPYKVGNH
                     RHTPEIEQILGEVADKPANVLFQPHLCPMDQGMMCTICATPTAAVKDDSQLVDVMKNA
                     YDAEPFVRVRTDTLPATKNVMNTNFCDVAVKLAKGRVLIFSTIDNLLKGASTQAIQNL
                     NVMFGLDETTGLY"
     gene            891..2093
                     /gene="argJ"
                     /locus_tag="PLK08_00250"
     CDS             891..2093
                     /gene="argJ"
                     /locus_tag="PLK08_00250"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPS51962.1"
                     /translation="MNNRHITIPSGFKAAAVACGIKKSGKHDLAIIAADTDASAAIVT
                     TSNQIFGAPIAWDRQILPKGYGKIRGMVINAGNSNVCTGKQGFCDAKAMAARTAKHLG
                     SKPEKVLVASTGVIGRAMPMANVTAGIDSAAPGLNTKSDHRVVEAISTTDTRYKSAVV
                     QIRLGGKNVTVAGIVKGSGMIAPSLATMIGVITTDAAVAPALLYKLLRQAVRTSFNAV
                     TVDSDQSTSDIVAIFASGCAGAKEVKDGTAECKKFFAAVQEVCDALAFSIAADGEGAT
                     RVIEISVTGAHNDKEAQMAAKSVADSPLVKTAVHGADPNWGRIAMALGKSAAKVKAEK
                     LIIKIGGTKIFAKGTGCKCDLKKISEMMKQNVVKIDCELGQGKGKFTALTCDLSREYI
                     SINADYTT"
     gene            2372..2866
                     /locus_tag="PLK08_00255"
     CDS             2372..2866
                     /locus_tag="PLK08_00255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPS51963.1"
                     /translation="MKIRPINVKDIPEVLQIMLDNWDGVMAEHHSVEIVRKFRSEVSA
                     DWLKRQMDWKRIFVVEKDDRIVATGALADFGQPGNPKLCVSQFFVSSHLHGRGIGKCL
                     LKYLTETAKKDGTQQLHVPSSRNAIPFYKSAGFVTDPVQTDAADEITWMSMDISSPGA
                     SDKS"
     gene            complement(2904..3641)
                     /locus_tag="PLK08_00260"
     CDS             complement(2904..3641)
                     /locus_tag="PLK08_00260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPS51964.1"
                     /translation="MRRFYQPCLCVGSQELQRDEAHHAANVLRLKRGDTLELFNGDGL
                     TGDAEILEITKKTVLVNVPQVCECLRRGPLLHIAFAVPKGKRLDWLLEKATELGVASL
                     WPVAFEYSVAGGDELGDSKLSRWEQHCISAAKQCELNFMPKLHDIMPLDDFLMNSPSA
                     KLKIVGDCVDGTPNISEKMSKMMADNSAADICMLIGSEAGFTADERKKIADAGFKSVR
                     LGETVLRIETAVIALAAAITAYCSVKS"
     gene            complement(3757..6153)
                     /gene="lon"
                     /locus_tag="PLK08_00265"
     CDS             complement(3757..6153)
                     /gene="lon"
                     /locus_tag="PLK08_00265"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPS51965.1"
                     /translation="MDNKNENEKISKKVIVTAQDESAQEEIQPAHQLPNELFVLPIRD
                     SVLFPGTVVPLTIQREKSRRLLTDLLPDNKILLAVCQKDPAIENPVADNLYDVGTAIM
                     VLKLLRMEDGTQNVIVQSLGKARITQWTQKDDNLVAVIEPLADHIKKTPQNEALLHTV
                     FTAAQRVVELSPNIPNEAKLVLNNIDTPSSLADFLAANLPMDTSERQLLLAETDVVKR
                     LEMINVRLQKQLEILELSDKIRSEVKEQVDKHQKEYFLNEQLKAIQKELGQTDDKTVE
                     LAELKEQLTRAKLPDAVQKEADREMSRLEKIPSISPDYNVIRTYLEIISELPWSVSTV
                     DKLDVKAAAKVLNADHYGLEKVKRRILEYLAVRKLSPESRGPILCFVGPPGVGKTSLG
                     QSIARAMGRKFIRMSLGGMRDEAELRGHRRTYIGAMPGRVIQELRKAGTNNPVFMLDE
                     LDKVGNDFRGDPSSALLEILDPAQNNTFQDHYLNLPFDLSKVLFIATANYMGTVPPAL
                     RDRMEVIELPGYTSQEKLEIAKRYLVKRQRQENGLKNGNIRFPDEVLKLIINDYTREA
                     GVRELERQIGAICRGVAARVATGRVEKMAVTENLVRKILGPRQYESELALRQAQPGVV
                     TGLAYTPVGGEILFIESAVYPGNGKLVMTGQIGDVMKESAQAALSLVKGRLAELKLDA
                     EFFNKHDIHIHVPAGAIPKDGPSAGCAMFSSLMSLLMKRCAKVDVAMTGEITLRGIVL
                     PIGGVKEKVLAAKRAGIKTVILPEGNRKNLVDVPAIVKKSLKFRFVRNVDQVLRLVLK
                     "
     gene            complement(6155..6592)
                     /locus_tag="PLK08_00270"
     CDS             complement(6155..6592)
                     /locus_tag="PLK08_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPS51966.1"
                     /translation="MIHDCQTEKKGSFHSLHDSIKRTIDEVISGRYRQFCHSGEESWQ
                     PAVNIYSSDECNYIIVDIAGMALDCMNVEASSGELVISGERMSPQMPRHHGAARVLHM
                     EIDHGKFCRKIKLPDEADLDAITAKYKNGLLTIEIPIKHDDEK"
     gene            complement(6776..8971)
                     /locus_tag="PLK08_00275"
     CDS             complement(6776..8971)
                     /locus_tag="PLK08_00275"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPS51967.1"
                     /translation="MNMLRTREETPLIIRSAAGMSPARKLSAGSSGGAGAGGFSGQDI
                     MRMLRRRKWLIIICLFIFLVISGVSSFLWNKMAPIYKAQALIVVYPPVASELALNVGP
                     TLGSASKSLLETRVKSIHSESVFENAIKSIKGTKWFEKNNADGEGLVKLMDAVDVGIV
                     PNTNFIYISMTGLDREDITVIVNAIAEAAEKDASLMQSTDKQKQIERLQETVQKKSQE
                     KEEAVRIIKTLRAGPTRLDPSTIQIKHGMLVKEMELANKQYAQALSMWDGMKSRTDKE
                     IAEMPEIQYMVLNNPEVSILKNRILNIEFELDYALKTLGEKHNTVKSLKNRLEIANTT
                     YKEKYDEVMAKTIKETKELREQEFIASEKYREDISTQLNESDRAMKELHNIETALSDA
                     ANTVSNCDQEINALNYRIMDLRLLLKGETPVVLQHKARKPIVPVYPQMPIMIALGAFL
                     GLFFGGGLALLLEMLDTSIKSAEDVVNKVQLPVMGIVPHEDDVDEDIDDMRLAFLSHP
                     NTIICEAFRQIRTTLLFSGQVGQHSSVLITSPMPSDGRTTVTLNLASHLVKGGMKVLV
                     VEANFRQPVIRHLFKNDCPKNGLSSILQGTANWEECLFTVNNEFDVISAGSIPQNPTE
                     LLGSNEMASLAEELASRYDMVLYDSSPCLIVSDASVLSTRVDGVLFTFRAGVSTFNMG
                     QRSRAMFEQLGAHIYGAVINGMKVNMEGSVRKDYETFYEYSSTPQSPAV"
     gene            complement(8968..13437)
                     /locus_tag="PLK08_00280"
     CDS             complement(8968..13437)
                     /locus_tag="PLK08_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS51968.1"
                     /translation="MAKKKRLNSRMLVLLGVMAAILIICLTYIYFRSLPQNPEFYANK
                     AAQLMSAPQKNYLEAINCLRLAAEYSSGIKRNEYLYRQAELMLEYASRGVDLDDADRE
                     QAYSSGLGLLQKILQSDENYVPARKLLAQHRWAIAVRARQWHLWENYIQQMDKLIEAE
                     DTAENYFRRGYAKSALAMTDPSFNDAAIEDFNKAIELDKEKLQYRIALARFYELIKDT
                     ASAELTYKKSMEANPDKADIRIEYANFLRRKNENKLAQRVIIDAIKCEPNNAAGYVAM
                     AEMALAEKDYKTAQNALEKAKKIDPAMVYAYYYQANIARAQKRLKDAADALREGLSAV
                     ENRDMTSESGKKLDANKAKALLNYWLADIMLDIYQTSNDPKEREAALQEAKACNTVLL
                     KLSPDDPRQLKIDGRLAMIGQNYAKAKDCFEHSLEMETDLRTAAWLINIYRVLNLPTR
                     GETLSKKILAVPGEEDNVYFLLRYAEFRIDANDFTKATEYLSQVLAKEPDNRQAQELM
                     AAIELVRAKRIENQKISPIARALLLRKAENFVVLNKLEQAQNILESLLRSNPKDMVVM
                     SRLLSVYTQNGQTEKAAGTIDASLAKNPNNEDLKRWKALLNEKSPEKRCDIELNFAGK
                     IQDPLNRALSSWSICKRYGQEKSAAKYLAEARKIKPDNQIVIEGYFLQALANKDYELA
                     RKEIEPLEKRDPLDWNLYMGRLLYAQNNFESAATYLQGALKYQSYNQPARLMLADCFL
                     QLNETEKAKQEFQRCFSNDSKDIRAMVGLAKIAMATHNVEEHDRWVELAYNYPEGKVD
                     SYIREEYLRMNVDNKNPAQAIETRERLFEKEPSNLENALRLAFLYERKKDIAKAREKF
                     EYIYANVADKISFAPLLAEFYMRQKETTKADELFNSLIKQAKTANDKIAAYVAYGSFL
                     SGMDQQAAMSMFEKAKSIDPNNTMPYKALSNLLGVQAIQLSQQGKLKDTQQKWQAAVK
                     QLQEVIRMTPEDKQVRQTLYRIYIDAQMYKDAEDGFTMLVHEDPNDVRARLGLGLTYL
                     REGKTGKAVTEFKNVIEMTPDNPDAYAFRAEAYKSQGDLVMAIADLKRACQLSSQMLL
                     RMDLANLYLANDQSDMAEVVYSEIIDTPSTDKYFPAYQQLLRIGLAQQKWPLVEKICN
                     KGEKLFPESPLFCLTRAEMWNRRDNQQSMLEFLNKAVKIAPTDSVVVGAYFKGLMEGR
                     KYDALLKVADYYQNQANFVSLSHAYRGMVLAARKDAGALNEFLEALKIAEKSTDVLNT
                     VGMMAQVYGKAEILKLADNIIAAKPDNWQIFMLLGNYCVELDGYKQAHGYYIKALNLA
                     GDVNSRVAVLLQMAHMYEITRDYESVEKTYLAILNMDANNTVALNNLAYLYVDIMKRP
                     DKAMPMIERAMTLMPGNLNLMDTYGWVLAKNGDYNKASQQLSEVVRRGKAGPESLYHM
                     GYVLERTNQMREARDYYRRSLELVADKKSKMYAEIAEAYNRVENELKKRSEVK"
     gene            complement(13603..14472)
                     /locus_tag="PLK08_00285"
     CDS             complement(13603..14472)
                     /locus_tag="PLK08_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51969.1"
                     /translation="MSNRKGWLHFGIAVGILLISIVSWNVMTAKLRYFFRKEAVPWPA
                     SVKVSELEFRNISLPKQFGPYRMIEDDGILDRDENGNVVKDGKPDGEIYYREDILETL
                     GIGSSLDEERVKERKSNWYVSRYYIDAREPENSPYKCWQLDLTFYTGSEVTVPHVPDI
                     CVLAGGASIVSRENLFITDVAGVPEPWDKRTPFTGLHYERNDNNAYSYVQYYLFSVNG
                     LPETDRDAARIELTALSNRYVYYAKLQFFPRAVIRDKKQADERAKDFLRHCIPGVLRE
                     LPSREYISSLNKK"
     gene            complement(14469..15506)
                     /locus_tag="PLK08_00290"
     CDS             complement(14469..15506)
                     /locus_tag="PLK08_00290"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HPS51970.1"
                     /translation="MKVKTKAVIVSDLTEENIKVENSGETVKQSLADMIKGQDEAAKA
                     PSLPFYVWIKIIVLGALVLLFFRDEVIQLWKTWRGNVNWSHGFIIPFFSCYLIYAWWD
                     KITKIPCRIWLPGIFVILLAMVGKAYGLIVLHNMWSAQLMLPLMIWGLVLYLCGPRMA
                     MVLFVPIFFLELAMPLPDRLYNLMSLPMQNLSARMATSILQLFGVQISVSASYMQVVS
                     ISGVHHSLEVAEACSGMRSLMAFIALGVAMAYLDERPFWERLVIMLAGIPIALICNIL
                     RVTLTCSMFVLDMKELGEGFMHNMMGMALLVPALILLFLLAKLLDGLYVEEDDDVSDA
                     SGGDDAAEVKS"
     gene            complement(15518..17098)
                     /locus_tag="PLK08_00295"
     CDS             complement(15518..17098)
                     /locus_tag="PLK08_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPS51971.1"
                     /translation="MDKMNWLNGLTVTVMFAFAAAGCNSWTDDYLKSSKYRFLSPEKV
                     VERPNEAEVNYIISAMGIEKDDALFPNARRPQPSDWEYSATDYCLAPGDFIEVSIMGL
                     YAEGVETTIRREIAESGYIDLPLLDVRIMAEGMTQMDLVKAIEQAYSKNVIREPQVSV
                     TIMSRSKRTFSVLGAVGRPGTYQITKKDMRLLDVMALCGGVYQSDIEYIYVIRQAQAI
                     RKGDDDAVKTQETTPKRHSEKAKKLQEVGDLMNGPTSSVKPVADVRDVQNLAAVPDDS
                     AASPAAGQSQIEGKQLASAKPTEYQWKFVDNRWVKVPVGGETPAAKTPAGKADNVSLD
                     MPTPKTPDATATTPKKDPFDWADLEKSDLVEVIAINRKNLDQGDYRQNIVIHDNDIIR
                     IPPIELGEFYLMGEVMRPGVYALTSKGITVKMAVAAAGNLGPMAWPENSIIIRRVGKN
                     QEQMIPVNVEKIFKGEDPDVYLKPNDILAVGTHWSSTFLAVIRNSFRMTYGFGFIYDR
                     NFGDPYEPSLDSKRFSRW"
     gene            complement(17283..18812)
                     /locus_tag="PLK08_00300"
     CDS             complement(17283..18812)
                     /locus_tag="PLK08_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51972.1"
                     /translation="MMTTGKINGRMGMISVRPFFLLIIFAVPIIQGGCEFDFSRSANE
                     ETKKAAVTGKDDKTPTDELLLTGLAAADGQRGFCVQPSIYRPGSELQELDGQAVKAVL
                     PPKAWLTYQAGRKQPLDIANFTHLAFDMEYSGEIGVCQVIIDSDSGQWTSRPIAVSRG
                     VNQVILGIHQLWRSVDFDVHAVSALKIGFPDAELNRGFILRNVRFLRDLRNVSPSPAG
                     IELQEIGWDYILKFCDSGERLELFRSDDGLWRMGLAQPVLRLARFGGRKLAESGGEEL
                     DAMGKDRFGEVDLLEHNIVRIQLADNWYFNGFEGGIKAFSNRYVRWTYTIYGDGRCVV
                     AAELDNSGGPELEKVEISVRSQRAAWHGKGMSGAMSDDSFIGPMGKWTFMTVIGDKNR
                     MRFFQNYLNPAKIKPILSQKGIFAPGDADRDGFDESQGCYCLAGRPAGCKFEVICDEK
                     PLFLPIFKIFGPWKKLPTVVVEGRRIEKKVLLGDGSVLFELPGEYRRNFTVEVLGEIR
                     R"
     gene            complement(18831..>19766)
                     /locus_tag="PLK08_00305"
     CDS             complement(18831..>19766)
                     /locus_tag="PLK08_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPS51973.1"
                     /translation="VGCKPTYGRISRYGLVAFGSSLDQIGPMTRTVRDAALLTQVMAG
                     HDGHDATSLPNAVPDYAAAMNTPVKNPKIGLPREFYTDALGGEVRQSIEKAIEVYKSL
                     GAEFVDVSLPHSRIDMDAAGNLSSYAVACYYIVCTAECSSNLARFDGVHYGYRTRQNV
                     SDIVELYCKSRAESLGDEVKRRIMLGNYALSSGYYDAYYNKALKVRRLIADDFSAAFR
                     QCDVLLCPTSPTTAFRFGEKSANPIEMYMADIYTISLNLAGLPGMSLPCGLDSQGMPI
                     GMQLIGPVLSEASLFGIAEMYEKAAKFDAKPAMLQ"
BASE COUNT         4722 a         5346 c         4592 g         5106 t
ORIGIN      
        1 ggccaagcag gttaaaatgg cccagcagcc ctattcgccg gatacagtgg cggctctcaa
       61 gcctgacgta gctcttcttt gtgtgccgca ccagatagcg atgagctatg tgccgcagtt
      121 gttggctaag ggcgttaaaa taatcgactg gtcggctgat tatcgcatca ggaacgccgc
      181 cgtttatgaa aaatggtact gcgagcacac cgacaaaacg tcgctcgcac acgcagttta
      241 cggcctgccg gaactttatc gtaaccagat taagactgca aagttggtgg ctaatcccgg
      301 ctgttacccg acgtgttcga tacttgccct tgcgccgcta ttgcgggccg gcctgattga
      361 atccgaaaac atattcatat ccgccgtcag cggagtcacc ggcgcaggca gaaaggcaac
      421 gctcgaaaac cattaccctg agcggaatga aaatttcgag ccttacaagg tcggcaatca
      481 tcgccatacg ccggaaatcg agcagattct cggcgaagtc gcagacaagc cggctaacgt
      541 tctgtttcag ccgcacctct gcccgatgga tcagggcatg atgtgcacca tctgcgccac
      601 ccccaccgca gccgtaaaag atgactcgca actcgttgac gtaatgaaaa acgcctacga
      661 cgcagagccg ttcgtgcgag tccgtaccga cacactgccg gcgacgaaaa acgtaatgaa
      721 caccaatttc tgcgacgtag ccgtcaaact cgccaaaggc agagtgctta tattttcaac
      781 gatagacaat ctcctgaaag gcgcaagcac gcaggcgata cagaacctca acgtcatgtt
      841 cggcctggac gaaactaccg gcctttatta acaaaaataa cgggaacaac atgaacaaca
      901 gacacataac gattccgtca ggattcaaag ccgccgccgt agcatgcggc ataaagaaat
      961 ctggcaaaca cgaccttgcg ataatcgcgg cggatacgga tgcctcggca gctatcgtaa
     1021 ccacgtcgaa ccagattttc ggcgcgccga tagcgtggga ccggcagata ctgcccaagg
     1081 gatacggcaa aatacgcggc atggtcataa acgccggcaa cagcaacgtc tgtaccggca
     1141 aacagggttt ctgcgacgca aaggctatgg ctgccaggac ggcgaaacat ctgggcagta
     1201 agccggaaaa agtgctggtc gcatcaaccg gcgtaatcgg cagagccatg ccgatggcca
     1261 atgtgacggc tggcatcgac tccgccgctc ccggcctgaa tacaaaaagc gaccaccgcg
     1321 tcgtcgaggc aatctcaacg accgacacca gatataaatc agccgtcgtg caaatcaggc
     1381 tcggcgggaa aaacgtcacc gtcgccggca tagtcaaggg ttcgggcatg attgccccgt
     1441 cgctggcaac aatgataggc gtaatcacca ccgacgctgc cgtcgctcca gccctgctgt
     1501 ataagctgct cagacaggct gtgcggacat ctttcaacgc agtcaccgtc gacagtgacc
     1561 agagcaccag cgacatcgtg gcgattttcg cttccggctg tgccggcgcc aaagaagtca
     1621 aagacggcac ggcagagtgc aagaaattct tcgccgccgt tcaggaagtc tgtgacgcgc
     1681 tggcgttctc gatagccgcc gacggcgaag gcgcaacccg cgtcatcgaa atcagcgtca
     1741 ccggcgcaca caacgacaaa gaagctcaaa tggcagctaa aagcgtagcc gattcgccgc
     1801 tggtgaaaac cgccgtccac ggcgcagatc caaactgggg cagaatcgcc atggcactcg
     1861 gcaaaagcgc agccaaagtg aaggctgaaa aattgataat caaaatcggc ggaacaaaga
     1921 ttttcgccaa aggcacaggc tgtaagtgcg acctgaaaaa aatcagcgaa atgatgaagc
     1981 agaacgtggt gaaaatcgac tgcgaactgg gccagggcaa aggaaaattc accgccctga
     2041 cctgcgacct cagccgcgaa tacatttcca tcaacgccga ttacacgact tagcgcatat
     2101 taaacttgac tttgcgcttg ttgctgtttg cggcgtcggc tggctgcgtc aggctgcatc
     2161 ggaaaccata taaggttgcc tgcttcgcct tccttgccag cctcgtcctc aacgacgcaa
     2221 caaggcgcaa tttcaaattt aacatgctct agaaaatgtt tcctgtaaaa tcacggatgg
     2281 aatgtgaggt ttttccgcaa tacggaaaag ctgccgccgt ctgtaattat gcaatacttt
     2341 cgatactgtt aaaacggaaa ggaaacgaaa aatgaaaatt cgtcccataa acgtcaagga
     2401 cattcctgaa gttttgcaga taatgcttga taactgggac ggcgtgatgg ctgagcacca
     2461 ttcggttgag attgtgcgaa aattcagaag tgaagtatcc gccgactggc tgaagcggca
     2521 gatggattgg aagaggattt ttgtcgttga gaaagatgac agaatagttg ccaccggcgc
     2581 attggccgac ttcggccagc ctggcaatcc gaagctctgc gtatcgcaat ttttcgtaag
     2641 ttcacatctg cacggacgcg gcataggaaa atgcctgctg aagtatctga ctgaaacagc
     2701 aaaaaaagac ggcacacaac aattacacgt tccgagcagc cgcaacgcca taccttttta
     2761 caaaagcgcg ggttttgtta cagaccctgt acaaaccgac gccgcagatg aaatcacatg
     2821 gatgagcatg gatatttcct cccctggcgc atcagataaa tcgtgaattt caatacggca
     2881 atatgccgcg aaaaatgccc aactcaggat ttgacggaac aataagccgt aatagccgca
     2941 gccagtgcta tcaccgcagt ttcaattcgc aaaacagttt cgccgaggcg gacgctttta
     3001 aaaccggcat cggcgatttt tttgcgctcg tcagccgtaa agcctgcttc agagccgata
     3061 agcatgcaaa tatcggctgc ggaattgtca gccatcattt ttgacatttt ttctgaaata
     3121 ttaggcgtgc catcaacaca atcgccgact attttcaatt tcgccgacgg cgagttcatc
     3181 aaaaaatcat ctagcggcat tatatcgtgc aacttcggca tgaagttaag ttcgcactgc
     3241 ttggcggctg atatgcaatg ctgttcccat cgggacagtt tggaatcgcc aagttcgtcg
     3301 ccgccggcaa cgctgtattc gaatgccacc ggccacagcg atgccacgcc aagttcagta
     3361 gccttctcca gcagccaatc cagccgctta cccttcggca cggcaaaagc aatatgcaac
     3421 agaggcccgc gtcgcaaaca ttcgcaaacc tgcgggacat ttacgagcac agtctttttg
     3481 gtgatttcca atatctcggc gtcgccggtc agcccgtcgc cattgaaaag ttccaaggta
     3541 tcgccacgtt tcagccgcag aacattggcg gcatgatggg cttcatcgcg ttggagttcc
     3601 tgtgacccga cgcaaaggca cggctgatag aaacgacgca ttctgctttc ctgtctgctc
     3661 atggccggtg agtataacgc atcgcagccg tttcgggcaa gcgatattcc ggcgccgcat
     3721 tgccgcgtga cattccacgc actgccgcag cactttttat ttcaacacca gccgcaaaac
     3781 ctggtcgaca tttctgacga atctgaattt aagcgatttt ttcactatag ccggcacatc
     3841 tacaaggttt ttgcgattgc cctccggcag aattaccgtt ttgatgccgg ctcgcttggc
     3901 agccagaact ttctccttga ccccgccgat aggcagaaca atgccgcgaa gggttatctc
     3961 gcccgtcatg gcgacatcga ccttcgcaca gcgtttcatc agcaggctca tgagcgacga
     4021 gaacatggca cagccggctg acggcccatc tttgggaatc gctccagccg gcacatgaat
     4081 atgaatatcg tgtttattga aaaattccgc atctagtttg agctcggcca gccggccttt
     4141 taccaaacta agcgcagcct gagccgactc tttcatgacg tcgccaatct ggccggtcat
     4201 cacgagtttg ccgttgccag gatacaccgc cgactcaatg aaaagtattt caccgccaac
     4261 cggcgtatat gccagccctg taaccacacc cggctgtgcc tgccgcagcg ccagttcgct
     4321 ttcatactgt ctgggcccca gaatctttcg tacaagattt tccgtaacgg ccattttctc
     4381 aacacggccg gtggcaaccc tggcagctac gccgcggcat atcgcgccta tctgacgctc
     4441 aagctcacga acgccggctt cacgggtata atcgttgatt atcagcttaa gcacctcgtc
     4501 aggaaaacgg atgttcccat tcttcaagcc gttctcctgc cgctgccgct ttacgaggta
     4561 tcgcttggca atttcgagct tttcctggct ggtataacca ggcagttcga tgacttccat
     4621 tctgtcccgc aacgccggcg gcacagtgcc catgtaattc gccgtagcga tgaacagcac
     4681 cttgctcaaa tcgaacggca aattcagata atgatcctga aaagtgttgt tctgcgccgg
     4741 gtccaatatc tccagaagcg cactgctcgg gtcgccgcga aaatcattgc cgaccttgtc
     4801 cagttcgtcc agcatgaaga caggattatt tgtgccggct ttgcgtagtt cctgaattac
     4861 acggccgggc atggcgccaa tataggtacg tcgatggccg cgaagctcag cctcatcgcg
     4921 catgccgcca agactcatgc gtatgaattt tctgcccatg gctctggcga ttgactggcc
     4981 cagcgatgtt ttgccgacgc cgggcggccc gacgaaacat aaaatcggcc cacgggattc
     5041 tggcgaaagt ttccggacag ccagatactc aaggatgcgt cgcttgacct tttccagccc
     5101 gtaatggtcg gcattgagca ctttggcggc tgctttcacg tcaagcttgt caaccgtgga
     5161 aacactccac ggaagctcgc tgattatctc gagataggtg cgaatgacat tgtaatcggg
     5221 tgaaatggac ggaatttttt ccagccggct catttcacgg tcggcttcct tctgaactgc
     5281 gtcaggaagt ttggcgcgag ttaactgttc cttcaactcg gctaactcga cagttttgtc
     5341 atcggtctgg ccaagctcct tctgaatcgc cttgagctgc tcgttcagga aatattcctt
     5401 ctgatgcttg tcgacctgtt ccttgacctc ggaacgaatc ttgtcggaaa gctccagaat
     5461 ctccagctgc ttctgcagcc ttacgtttat catttcaagc cgcttgacca catcggtttc
     5521 ggcaagaagc aactgacgct ccgatgtgtc catcggaagg ttggcagcca gaaaatcggc
     5581 aagcgaagag ggagtatcta tattgttcag caccagtttc gcttcattcg gaatattcgg
     5641 cgaaagctcc accacccgct gggccgcagt aaaaaccgta tgcagcaacg cttcattctg
     5701 cggagttttc ttgatgtggt cggcaagcgg ctcaataacc gcaaccagat tgtcatcctt
     5761 ctgcgtccac tgagttatcc tggctttgcc gagactctga acgataacat tctgagtgcc
     5821 gtcctccata cgcagaagct tcagtaccat gatagccgtg ccgacatcat acagattatc
     5881 cgcaacggga ttctcaatgg ccggatcttt ctggcagacg gcaaggagta ttttattatc
     5941 tggcagaaga tccgtcagca gccgcctgga tttctcccgc tgaatcgtca gcggcacaac
     6001 cgtacctgga aacagcaccg aatcccgaat cggaagaaca aaaagctcgt taggcaactg
     6061 atgagccggc tgaatttctt cctgcgccga ttcgtcctgc gccgtaacaa tcactttttt
     6121 gctgattttt tcgttttcgt ttttgttatc catcttattt ttcatcgtca tgttttatgg
     6181 ggatttcaat cgtcaaaagg ccattcttgt atttggcggt aatcgcgtcc aaatccgcct
     6241 catccggcaa ttttatcttg cggcagaact tcccgtggtc tatctccata tgcaacaccc
     6301 tcgccgctcc atgatgcctg ggcatctgcg gcgacattcg ctcgccggat atgacaagct
     6361 cgccgctgga cgcctccaca ttcatacaat caagcgccat gccggcgatg tcaactatga
     6421 tataattaca ctcatcgctg gaatatatgt taaccgccgg ctgccaggat tcctcgccgc
     6481 tgtggcagaa ttgtcgatat cggccgctta tgacttcatc aatagtgcgt ttgatactgt
     6541 cgtgcagcga atgaaagctg cccttttttt cagtctgaca atcatgaatc atagcatccc
     6601 accttcccgt gccagcgagg catttattga acagacatat tatatattcc ttaatcgccg
     6661 cacgcaatat gatgccaata aaaaggcctc tctgaacaat cctgagaggc ctttaaatat
     6721 ttccggattt cctgaaattt tcaaataaag ctgcgacggc agccggcaaa ccgaatcata
     6781 ccgccggcga ctgtggcgtg ctgctgtatt catagaaagt ctcgtaatcc ttccgaaccg
     6841 agccttccat atttaccttc atgccgttaa tgaccgcacc ataaatatgt gcccccaact
     6901 gttcaaacat agcgcgggac cgctggccca tattgaacgt gcttacgccg gcacggaaag
     6961 taaacaacac tccgtcaacc cgcgtgctca aaaccgaagc gtcggaaact ataagacatg
     7021 gcgatgaatc atagagaacc atatcataac ggctagccaa ttcttcagca aggctggcca
     7081 tctcgttcga gccgagcagt tccgtgggat tctgaggtat agaacccgcc gatatgacgt
     7141 caaattcatt attgacggta aacaggcatt cttcccaatt cgccgtgccc tgtaatatcg
     7201 aactcaggcc attcttcggg caatcgtttt tgaaaagatg ccgaatcaca ggctgacgga
     7261 aattggcttc aacaaccaga accttcatgc cgcccttgac cagatgactg gccaggttca
     7321 gcgttaccgt agttctgccg tccgaaggca tcggacttgt tatgagtaca ctgctatgct
     7381 ggcccacctg gccggagaac aacaacgtcg tacgtatctg ccgaaacgcc tcacagataa
     7441 ttgtgttggg atgcgacaga aacgccagac gcatgtcgtc gatgtcctcg tcgacatcgt
     7501 cttcgtgcgg aactataccc atgacgggca actgaacctt gttgacgaca tcttccgcag
     7561 acttgatgga ggtatcgagc atttccagca acagcgccaa tccgccgcca aagaaaagcc
     7621 caagaaaagc gccaagggca atcataatcg gcatttgcgg ataaacaggc acgataggtt
     7681 ttctggcctt atgctgaaga actaccggcg tttcaccctt gagcaacagc cgtaagtcca
     7741 ttatacgata attcagggcg tttatttcct gatcgcaatt agacacggta tttgcggcat
     7801 cgctcaatgc cgtttctata ttgtgaagct ccttcatcgc acggtcagac tcgttcaact
     7861 gcgtggaaat gtcttcacga tatttttccg atgctatgaa ctcctgctcg cgcaattcct
     7921 tggtctcttt tatggtcttc gccataactt catcatattt ttctttatac gtagtattcg
     7981 caatctccag acgatttttc aacgatttga cggtattgtg tttttcaccc aacgtcttga
     8041 gcgcgtaatc aagttcaaat tcgatattga gtatgcgatt tttcagtatg ctgacttccg
     8101 gattattcag caccatgtac tgaatttccg gcatttcggc aatttcctta tcagtacggc
     8161 ttttcatgcc atcccacatg ctcaatgcct gggcatactg cttattcgcc agttccatct
     8221 ccttgacaag catgccgtgt tttatctgaa tggtggaagg atcaagcctt gtcgggccgg
     8281 ctcgcagagt cttgattata cgaacagcct cttccttttc ctggctcttt ttctgcacag
     8341 tttcctgcaa acgctctatc tgtttctgtt tatcggtact ttgcatcagc gaagcatctt
     8401 tttcggcagc ttccgcgatg gcattgacaa taacggtaat atcttcccta tccagccccg
     8461 tcatagaaat atagatgaaa ttcgtattcg gcactatacc gacatccacg gcatccataa
     8521 gtttcacaag gccttcgccg tcggcattat tcttttcaaa ccatttcgta cccttgatgg
     8581 actttattgc gttttcaaaa acggattccg aatgtattga ttttactcgc gtttcgagca
     8641 atgatttgct ggcagaacca agtgtcggcc ctacgttcaa tgcaagctcg gacgccaccg
     8701 gcggatacac cactatcaat gcctgagcct tgtatatcgg cgccatcttg ttccataaaa
     8761 atgatgaaac gcccgatatt accaaaaaga tgaaaaggca gatgattatg agccattttc
     8821 gccttctgag catgcgcata atgtcctggc cggaaaagcc gccggctcct gcgccgccag
     8881 aactgccggc ggataacttt ctcgccggac tcatgcctgc ggcacttctt attatcagcg
     8941 gcgtttcttc acgggtacga agcatattca tttaacctca ctcctttttt tcaactcatt
     9001 ttcgacccga ttataggcct cggctatttc tgcatacatt ttggactttt tgtcagccac
     9061 caattccaat gacctgcgat aataatcacg agcctctctc atctgatttg tcctttcaag
     9121 gacgtagccc atatgataca aagattcagg gccggctttg ccgcgacgga caacttcgga
     9181 aagttgctga ctggctttat tatagtcacc attcttcgcc aaaacccacc catatgtatc
     9241 catcaagttc aaattgccag gcatcagagt cattgctctt tcaatcatgg gcatggcttt
     9301 atccggccgt ttcatgatat cgacatagag atacgccaga ttattaagtg ccaccgtatt
     9361 attggcatcc atattcaata tcgccaggta agtcttttcg acgctttcat aatcccgcgt
     9421 tatctcgtac atatgtgcca tttgaaggag cacggcgacc ctggagttga catcgccggc
     9481 taaattcagg gcttttatat aatagccatg tgcctgctta taaccgtcta attccacaca
     9541 atagttgccc aaaagcatga atatctgcca gttatccggc ttagcggcaa ttatattatc
     9601 ggcaagtttg agaatctcgg ctttgccgta aacctgagcc atcatgccaa ctgtatttaa
     9661 gacatccgtc gatttttccg caattttaag ggcttcaaga aactcgttta atgcccctgc
     9721 atcttttctg gcagccagaa ccatgccgcg atacgcatgg cttaacgaga caaagtttgc
     9781 ctgattctgg taataatccg caaccttcag gagtgcgtca tattttctgc cttccatcaa
     9841 gcccttgaaa tatgcaccaa cgacaacaga atcggttggg gcgattttaa cggctttgtt
     9901 caggaactcc agcatggatt gctgattgtc gcgtctgttc cacatttccg cccttgtaag
     9961 gcagaacagc ggcgattccg gaaacagttt ttcacccttg ttgcaaattt tctctaccag
    10021 cggccatttc tgctgcgcca aacctattct cagaagctgc tgatatgccg gaaaatattt
    10081 atcagtgctg ggggtatcga ttatctcaga atacacgact tcagccatgt cggactggtc
    10141 attcgccaga taaagattgg ccaaatccat acgcagaagc atttgactgg acagctggca
    10201 ggctcttttc aggtcggcta ttgccatgac gagatcgccc tgcgatttat acgcttcagc
    10261 ccggaacgca taagcatccg gattatcagg cgtcatctcg ataacattct tgaattcggt
    10321 gacagccttg cccgtcttgc cttcgcgtaa atatgtcaaa cccaatccca gccttgctct
    10381 gacatcattg ggatcttcat gaaccaacat ggtaaagccg tcttcggcat ccttatacat
    10441 ctgcgcgtcg atgtatattc tataaagcgt ctggcgtacc tgtttatctt cgggagtcat
    10501 tctgatgact tcctgtaatt gtttgacagc agcctgccat ttctgttggg tgtctttgag
    10561 tttgccctgc tgcgacagct gtattgcctg gactcccaaa agattggata gtgctttata
    10621 cggcatggta ttgttcggat ctatgctttt agccttctca aacatgctca tggctgcctg
    10681 ctgatccatg cctgacagaa aagagccgta agcgacatac gcagcaatct tgtcgtttgc
    10741 agtcttagcc tgctttatca acgaattgaa aagttcgtca gcctttgtgg tctctttctg
    10801 cctcatgtaa aattccgcaa gaagcggggc aaacgaaatt ttatcagcca cattagcata
    10861 aatatattcg aatttctcgc gtgccttggc gatatccttt ttacgttcgt aaagaaatgc
    10921 cagtcgaagg gcattttcga gattggatgg ttctttctca aaaagtcgtt cacgagtctc
    10981 tatcgcctgg gcgggatttt tgttgtcgac attcatccga aggtattctt cgcggatata
    11041 tgaatctacc ttgccttccg gataattgta agccagttcg acccatcggt catgttcttc
    11101 cacattatgg gtagccattg ctatctttgc aaggccgacc atagccctga tatctttgga
    11161 gtcattggaa aaacagcgtt gaaattcctg cttagccttt tccgtctcgt taagttgcag
    11221 gaaacagtcc gccaacatca gcctggcggg ctgattgtat gactgatact tcaacgcacc
    11281 ctgcagatag gttgcagccg attcaaaatt attctgagca tataataatc tgcccatata
    11341 aaggttccag tcaagcggat cgcgtttttc cagcggttct atttccttgc gggccagctc
    11401 ataatctttg ttcgccaaag cctgcaggaa atagccttct atgactatct ggttatcggg
    11461 ctttatttta cgggcttccg caagatattt cgcggccgac ttttcctggc cgtatctttt
    11521 gcatatgctc caactgctca acgccctgtt gagcggatct tgaatcttcc ctgcgaaatt
    11581 cagttcaata tcacaacgtt tttccggtga tttttcattg agcagcgctt tccagcgttt
    11641 gaggtcttcg ttgttcgggt tcttcgcaag cgatgcgtca atagttccgg cggctttttc
    11701 tgtttggccg ttctgggtat agacgctcaa caatctgctc attacgacca tatccttggg
    11761 attgctcctg agaagcgatt ccaggatatt ctgtgcctgt tccagtttgt tcaaaacaac
    11821 aaaattttcc gcctttcgca aaagcagcgc tctggctatg ggcgatattt tctgattttc
    11881 gattctcttg gctcttacga gttctatagc agccatcaat tcctgagcct gcctgttatc
    11941 cggctctttc gcaagaacct ggctgagata ttcggtggct ttcgtaaaat cgttggcatc
    12001 aatacggaat tcggcgtagc gtaaaagaaa atacacgttg tcctcttcgc ctggaacagc
    12061 aagtattttt ttagacagcg tttcgccgcg agtcggcaga ttgagtactc tatagatgtt
    12121 tatcagccac gccgccgtcc gcaggtctgt ttccatttcc aacgaatgtt caaagcaatc
    12181 cttagccttt gcataatttt ggcctatcat tgcaagacgg ccgtcgattt tcaactgccg
    12241 cgggtcatcg ggcgaaagtt tcaatagaac ggtgttgcag gctttggctt cctgaagtgc
    12301 cgcttcacgt tctttggggt cattgctggt ctgatatata tccagcatga tgtcagccag
    12361 ccagtaattc aacagcgcct tagccttgtt cgcatccagc tttttgccgc tttccgacgt
    12421 catatcacga ttttcaacag cggagagtcc ttcgcgaaga gcatcggcgg catctttgag
    12481 tcttttttga gcgcgtgcga tattagcctg ataatagtac gcatagacca tggcagggtc
    12541 tatttttttt gctttttcaa gggcattctg agcggttttg taatcttttt ccgccagcgc
    12601 catttcagcc atggcgacgt agccggcggc attgttcggc tcgcatttga tggcatcgat
    12661 gattacccgt tgagccagct tgttttcatt tttgcgacga aggaaatttg cgtactcgat
    12721 tcggatatca gccttgtctg gatttgcttc catgctcttt ttgtacgtca attccgcaga
    12781 cgccgtatcc tttatcaatt cgtaaaacct ggccagcgcg atgcgatatt gaagtttttc
    12841 cttgtccagt tctattgcct tgttgaaatc ctcgatagcc gcgtcgttaa acgacggatc
    12901 ggtcatggcc aaggcgcttt tcgcgtagcc gcgacggaaa taattttcag ccgtatcttc
    12961 agcctcgata agtttatcca tctgctgaat gtaattttcc cacaaatgcc actgccgtgc
    13021 acgaacggct atagcccatc tatgctgagc cagcaacttg cgagctggaa cgtaattttc
    13081 atcggactgc aatattttct gaagaaggcc caagccgctg ctgtatgcct gttcacgatc
    13141 ggcatcgtcg aggtcaacgc cgcgggatgc gtattcaagc atcagttcag cctgccggta
    13201 aagatattca ttacgtttga ttccgctgga atactccgca gccagccgta agcagttgat
    13261 tgcttcgagg taatttttct gcggtgccga catcaattgt gcagccttgt tggcgtaaaa
    13321 ctcgggattc tgcggcaaac tgcgaaaata tatataagtc aggcaaataa tcaggatagc
    13381 cgccataact cccaacagaa ccaacattct gctatttaga cgtttcttct tagccattgc
    13441 tcaccttccg aaaaatcatc tgatgaatat catccatttt tataatccga accagtaatc
    13501 aaaatcatct gctaaacatt ctctcgcaac tctgcccaaa accatcgaca cagccggaaa
    13561 ttacgtaagt tgtttattat aataaatcag caccattttt aatcattttt tgttcaggct
    13621 tgagatatat tcccgcgatg gcagttctcg gagcactcca ggaatgcaat gccgcagaaa
    13681 atctttcgca cgttcgtcag cctgtttctt gtcacgaatc accgccctgg ggaaaaattg
    13741 cagctttgcg taataaacat agcggttcga tagtgccgta agctcaattc gtgccgcgtc
    13801 acggtctgtt tccggcaagc cgttgacgct gaaaagataa tactgcacat aactgtacgc
    13861 attgttgtca ttccgttcat aatgcaagcc ggtaaacggc gtacgtttat cccacggctc
    13921 aggtacgcct gcaacatccg taataaacag gttttcgcgg ctcacaatgc tggcgccgcc
    13981 ggctaagacg caaatatccg gcacatgcgg cacggtaact tcactgccgg tataaaacgt
    14041 caaatcaagc tgccagcatt tgtatggaga attctccggc tctcgtgcat ctatgtaata
    14101 tctcgaaacg taccagttgc ttttgcgttc ttttactcgt tcttcatcga ggctgctgcc
    14161 gatgcccagc gtctccagta tatcctcacg ataataaatt tctccgtcag gtttgccgtc
    14221 tttgactaca ttgccgtttt catctctgtc caggattccg tcatcctcaa tcatacgata
    14281 agggccgaat tgttttggca gtgagatatt gcggaattca agctcggaaa ctttcacgct
    14341 agccggccac ggcacagctt ccttgcggaa gaaatacctc agcttagcag tcattacgtt
    14401 ccaggataca atggatatca gcaatattcc cacggcaatg ccgaaatgca gccagccttt
    14461 gcgattgctc atgacttcac ctccgccgca tcatcgccgc cggatgcatc actgacgtcg
    14521 tcgtcttcct cgacgtataa gccgtcgagc agcttagcca aaaggaacaa aagtatcagc
    14581 gccggcacaa gcagcgccat acccatcata ttgtgcataa atccctcgcc cagttccttc
    14641 atatccagga caaacatgct gcacgtgaga gtcacgcgga ggatattaca aataagcgct
    14701 atcggaatgc cggcgagcat tatgaccagt ctttcccaga aaggcctttc atccaggtac
    14761 gccatcgcca cgccgagggc tatgaacgcc atgagcgatc tcatgccgct gcacgcttca
    14821 gccacctcca gcgaatgatg cacgcctgaa atgctcacca cctgcatgta cgacgccgac
    14881 acgcttatct gtacgccgaa aagctgtaaa atgctcgtcg ccattctcgc cgaaagattc
    14941 tgcatcggca gtgacatcag attgtaaaga cggtccggca gcggcatggc aagttcgagg
    15001 aaaaatatcg gcacgaacag aaccatcgcc attctgggcc cgcagaggta cagcaccagt
    15061 ccccatatca tcagcggcag catcaactgt gccgaccaca tgttgtgcag aactatcagg
    15121 ccgtaagcct tgccaaccat cgccagcaga atcacaaaaa tacccggcag ccatatcctg
    15181 caaggaattt tcgttatttt gtcccaccat gcataaatca ggtagcagct gaaaaatggt
    15241 atgataaacc cgtgcgacca gttaacgttg ccgcgccaag ttttccataa ttgtatgact
    15301 tcgtccctga aaaacagcag tacgagagcg ccgaggacga ttatttttat ccagacatag
    15361 aatggcaagg acggggcctt ggcggcctcg tcctgtcctt ttatcatgtc tgcgagcgac
    15421 tgttttacag tttcgcccga gttttcgact tttatgtttt cttcagtcaa atcgctaaca
    15481 attactgctt tcgtttttac tttcatcagc ctgatgctta ccagcgtgag aaacgcttgc
    15541 tgtccagcga cggttcataa ggatcgccga aattgcggtc gtaaatgaat ccgaatccgt
    15601 aagtcatgcg gaacgaatta cgtatgacag caaggaatgt gctcgaccag tgcgtaccta
    15661 cggcaaggat atcattgggc ttgaggtaca cgtccgggtc ttcgcccttg aaaatctttt
    15721 ccacgtttac tggtatcatc tgttcctggt ttttgccgac gcggcggatg ataatagagt
    15781 tttccggcca cgccattggg ccgagattgc cggcagccgc caccgccatt tttaccgtta
    15841 tgcccttgct cgtgagggca taaacgcctg gccgcatgac ttcgcccatc agatagaatt
    15901 cgcccagttc gatgggagga atgcggatta tatcgttatc gtgtataacg atattctggc
    15961 gataatcgcc ctggtcgaga ttttttctgt tgattgcgat aacttcaacg aggtcgctct
    16021 tttccaggtc ggcccagtcg aacgggtctt ttttgggagt agtcgcagtt gcgtcgggcg
    16081 tttttggagt cggcatatcc agggacacat tgtcggcttt gccagcgggc gtttttgccg
    16141 caggcgtttc gccgccgacc ggtactttaa cccagcggtt atcgacgaat ttccactgat
    16201 actcggtcgg tttcgccgag gccagctgtt tgccttcgat ttgcgactgc ccggcagccg
    16261 gcgaagcagc actgtcgtca ggaacagccg ccagattctg cacgtcacgc acatcggcaa
    16321 cgggctttac gctgcttgta ggcccattca taaggtcgcc gacttcctga agttttttag
    16381 ccttttcgct gtgacgtttg ggcgtagttt cctgcgtctt gacggcatca tcatcgccct
    16441 tgcgaatagc ctgtgcctgc cgtatgacgt aaatgtattc gatatcgctc tgatacactc
    16501 cgccgcacag tgccatcacg tccagcagtc gcatatcttt cttggttatc tgatacgtgc
    16561 ccggccggcc cacggcgccg aggacggaga acgtacgttt gctgcgtgac ataatcgtca
    16621 ctgacacctg cggctcacgg atgacgttct ttgaatacgc ctgttcgatt gcctttacca
    16681 ggtccatctg cgtcatgcct tcagccatga ttcgcacgtc cagcagcggc aggtcgatat
    16741 aaccgctttc cgcgatttca cggcgaatgg tcgtttcaac gccttctgcg taaaggccca
    16801 taatgctgac ttctataaaa tcaccaggtg cgaggcaata atcggttgcc gaatattccc
    16861 agtcggacgg ctgtggccgg cgggcattgg gaaacagagc gtcatctttt tcaatgccca
    16921 tcgcactgat gatgtaattt acttccgcct cgttaggccg ttcgacgacc ttttcaggcg
    16981 acagaaaacg gtatttgctg gacttcagat agtcatccgt ccagctgtta cagccggcag
    17041 ctgcaaaggc gaacatcact gttactgtca atccgtttag ccaattcatt ttgtccataa
    17101 attacactcc gatccatgac acgttatggt gtcaggatta tatgcccttt taaaaaataa
    17161 cggtacacgg attatgtagt tatctcgact aatgatatga aaaaaatcgc aacagtccag
    17221 aattttttta acattttgct tcattctgcg cgatttaaac aactctggcc gacggctgaa
    17281 aatcagcggc ggatttcgcc cagaacttcg accgtaaagt ttctgcgata ctcgcctggg
    17341 agttcgaaca aaaccgagcc gtctcccagc agaacttttt tttctatgcg tcggccttca
    17401 accacgaccg tcggcaattt tttccagggg ccgaatattt tgaatatcgg cagaaacagc
    17461 ggcttttcat cacagataac ctcaaatttg cagccagccg gccggccggc aagacaataa
    17521 cagccctgcg actcgtcaaa gccgtccctg tccgcatcgc caggcgcaaa tatgcctttt
    17581 tgcgagagaa tcggtttaat tttcgcggga ttcagataat tctggaaaaa ccgcatgcga
    17641 tttttatcgc cgattaccgt catgaacgtc cacttaccca tcgggccgat gaacgaatca
    17701 tccgacattg ccccgctcat gcccttaccg tgccacgccg cccgctggct gcggacggat
    17761 atttccactt tctccaattc cggcccgccg gagttgtcca gctcagccgc caccacgcac
    17821 cggccgtcgc cgtaaatcgt gtaagtccat ctgacataac gatttgaaaa tgcttttatg
    17881 ccgccctcaa agccgttgaa ataccagttg tctgctaatt gtatccgtac gatattgtgc
    17941 tccaggaggt ctacttcgcc gaagcggtct ttgcccatgg catccagttc ttcgccgccg
    18001 ctttcggcaa gttttctgcc gccgaatcgt gcaagtcgca ataccggctg agccaggccc
    18061 atccgccaga gtccgtcgtc actgcgaaac agctccagcc gttcgccgct gtcgcaaaac
    18121 ttcagaatgt aatcccagcc gatttcctgg agttcaattc cggccggcga tggactcaca
    18181 tttcgcaagt cacgcaaaaa tcgcacgtta cgcaatataa aaccgcggtt gagttcagca
    18241 tcgggaaatc ctatctttaa ggctgatacg gcgtggacgt cgaaatcaac gcttcgccag
    18301 agttgatgta tgccgagtat cacctgattc acgccgcgac tgacagcgat gggcctgctc
    18361 gtccactgcc cggaatcgct gtctattatt acctggcaga cgccgatttc gcccgaatat
    18421 tccatatcga atgccaggtg cgtaaaattg gcgatatcca gcggctgttt tctgccagcc
    18481 tggtatgtca gccacgcctt cggcggcaga accgctttga cggcctggcc gtcaagttcc
    18541 tgcaactcgc tgccagggcg ataaatcgaa ggctgaacgc agaatcctcg ttggccgtcc
    18601 gccgccgcca aacctgtgag caaaagttca tccgtcggtg ttttatcatc ttttcctgta
    18661 acggcagcct tttttgtctc ttcgttcgca ctgcggctga aatcaaattc acatccgccc
    18721 tgaattatcg gcacggcaaa aattatcaat aaaaaaaacg gcctgacgga aatcattccc
    18781 atcctgccgt tgattttacc tgttgtcatc atagctgttt tcatcactct ttattgcagc
    18841 atagcgggtt tcgcatcaaa tttcgccgcc ttttcgtaca tttccgcgat gccgaacagg
    18901 ctggcttcgc tgagtaccgg cccgatgagt tgcatgccga ttggcatacc ctgagaatcc
    18961 aggccgcacg gcagactcat gcccggcagg ccggcgaggt tcagactgat tgtataaata
    19021 tcggccatgt acatttcgat tggattagcc gacttttcgc caaatctgaa agccgtcgta
    19081 ggcgacgtcg gacagagcag cacgtcacac tgccggaaag ccgccgaaaa atcgtccgca
    19141 atcagccgac gaactttcag cgccttgttg tagtatgcgt cataatatcc gctgctcagc
    19201 gcgtagttgc ccagcattat gcgacgtttg acttcgtcgc ccagcgattc agccctgctt
    19261 ttgcagtaca gctccacgat gtccgaaaca ttctgcctgg ttcgataacc gtaatgtacg
    19321 ccgtcgaacc tcgccagatt actgctgcat tcagccgtac atactatgta gtagcaggcc
    19381 acggcataac tgctgagatt gcctgccgca tccatgtcta tgcggctgtg aggaagagac
    19441 acatcgacaa attccgcacc gagagatttg taaacttcga tagccttttc tatggactgg
    19501 cgaacttcgc cgcccagcgc atcggtataa aattcacgcg gcaggccgat tttcggattt
    19561 tttaccggcg tattcattgc ggcggcgtaa tccggcacgg cattggggag gctggtggca
    19621 tcatggccgt cgtggccggc catcacctgc gtaagcagtg cggcatcgcg taccgttcgg
    19681 gtcatcgggc caatctggtc gaggctgctg ccgaaggcga caaggccgta acggcttatt
    19741 ctgccgtagg tgggtttgca gccgac
//
LOCUS       DAONFI010000004        17686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_3355_length_17686_cov_4.714877, whole genome shotgun sequence.
ACCESSION   DAONFI010000004 DAONFI010000000
VERSION     DAONFI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17686
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3355_length_17686_cov_4.714877"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLK08_00310"
     CDS             <1..703
                     /locus_tag="PLK08_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS51974.1"
                     /translation="MMFELKPGQAVVIFPFQYHAYANFATGTIDWLFTSFEWPNAVTP
                     SAFVKLKNTITTMSPEMLSFVERLIEHYTGEGDEDYRAMGVKLMTGALLNELLLAEET
                     HQTIRNRAGTSHTYELFQVVSQYIYANLDQPIQIRDVADYANISESHLRNVFTQKFGV
                     SIGSYIRRSKLIRACRLLETTESSIGEVAQACGFGSVYAFSRTFRQNIGQSPNQYRNR
                     PGSRRKRRPASTNVE"
     gene            complement(821..1900)
                     /locus_tag="PLK08_00315"
     CDS             complement(821..1900)
                     /locus_tag="PLK08_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPS51975.1"
                     /translation="MNIELKKNFKYSLLIPTSMGIRLTPADSQPMHCSDVFKMQATSA
                     ESNVASVASYLGLPVKVLTAFVKGSPIARFIKDNLAGRHMDYEGPEVPQGGPWGYRHQ
                     FNLADSGVGSRGPRVHNDRAGEVGRILNSKDFDLERIFCEEGVQIVHLSGLIAALSPE
                     TSKFCLEIAQTAKKYGTKISFDLNYRASFWVNREAELHKIFGEIAAAADILIGNEEDF
                     QLCLGVKGPEAGGKDIAAKIDGFREMINNARKEFPNASVFATTLREVVSANLHRWGAI
                     MFDGNDWHVIEPREITVLDRIGGGDGFVGGMLYAILKGWSPEKWAQFGWATGALATTL
                     LTDYAQPADEEQVWSIWNGNARVKR"
     gene            complement(2148..2969)
                     /locus_tag="PLK08_00320"
     CDS             complement(2148..2969)
                     /locus_tag="PLK08_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPS51976.1"
                     /translation="MSIDMFNVNGKTVAITGGAGILCGEMAKSLAACGARVAILDYNL
                     AGAQKVADAINAADGTAIAVKCNVLDKSAVVEAFEQTVKNFEHIDVLINGAGGNKKEA
                     TCVPPESTFFDLDADAIRWVFDLNCLGSIVPSQVFGKHMAQRNQGVIINISSMNAFRP
                     LTRIAAYSAAKAAVSNFTQWLSTYMCQNHSPNIRVNAIAPGFFLTDQNRFLLTNQDGS
                     KTRRGQQIIDHTPMSRYGEPADLVGTLVWLISDAAKFVTGIVVPVDGGFSAYSGV"
     gene            complement(2981..3634)
                     /locus_tag="PLK08_00325"
     CDS             complement(2981..3634)
                     /locus_tag="PLK08_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51977.1"
                     /translation="MKQMMLMAVLAAIIGGCDTNNMDVRKQYPDWPKWGWWERPAVAD
                     DETADESGKKEIADGRPDTEAERVELEESGKVKPIRKLTPLPDATITKEQLSKKIQFL
                     EQHREKVWRSVSMLREIDTLPLSEQNELRRKILENLDQWYEPMPVSPPNTKKPDWTDT
                     VIWDFMPSPMFCDAVNRWETIAQKNNLKFPANPSRRELLQYINDYVEGKLKPDRLRK"
     gene            complement(3804..5276)
                     /gene="gnd"
                     /locus_tag="PLK08_00330"
     CDS             complement(3804..5276)
                     /gene="gnd"
                     /locus_tag="PLK08_00330"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPS51978.1"
                     /translation="MSQPQADLGLIGLAVMGQNLVLNMNDKGFTIAVFNRTVSKVDDF
                     LNNQAKGTKIVGCHSIEEFIASLKRPRRVMMLVKAGQPVDDFIELLLPHLDTGDIIID
                     GGNSHFPDTIRRCKYVESKGLLYIGTGVSGGEEGARKGPSMMPGGSPAAWPAVKEIFQ
                     AIAAKAPDPKTGREEPCCDWVGEGGAGHFVKMVHNGIEYGDMQLICEAYNLMKTLGLT
                     NAEMCHVLADWNKGDLDSYLVEITRDILGYRDDAGREVLDSILDTAGQKGTGKWTAVA
                     ALDEGQPLTLIGEAVFARCLSAIREERVEASKILPGPAGKKIEGNRARIVADISKALY
                     ASKIVSYAQGYQLMRAAAKTYGWNLNYGGIALMWRGGCIIRSRFLGKIKEAFDKNPNL
                     VNLLLDSYFTSAVADCQQAWRNTVKLAVDMGVPTPAFSSALAYYDGYRSARLPANLLQ
                     AQRDYFGAHTYERVDRPRGEFFHTNWTGHGGNITATTYNA"
     gene            complement(5944..7440)
                     /locus_tag="PLK08_00335"
     CDS             complement(5944..7440)
                     /locus_tag="PLK08_00335"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPS51979.1"
                     /translation="MVAIASRKTNKGTKKKTTTRAEKAVKTAASGVKFRNYPQALKYL
                     FSLTDYEQMLRVRYNRDTFSLDRMEKLLKKIGNPHKKIKSVHIAGTKGKGSTSMMLAS
                     MLQHCGYKVGLYTSPHICDVRERISINGECIAKPELSALIAKISSHVEQMKKDRPTFF
                     EIFTAIAFMYFADKKIDIAVIETGLGGRLDSTNVIQPEVCGITSISMDHMHQLGNTLA
                     DIAREKAGIFKPQIPVISAPQNPIAKRVMKKVASEVKSPLFFAGDEIEFSYRVESSRT
                     EGCHTRVCLTTPSSKFEHLAVPVLGEHQAINCGLALALLDVLKQKGMKIDDKQALYGL
                     ERVYLPGRMEIIKTEPQILVDGAHNAASIKALIRGIGQHIPYDSMVMIFGCAADKDIR
                     GMMEQIATGADKLIFTKSSTSPRAADPHELAEVYAEMTDGRIAQVTNSLKQALEIAYS
                     AVSREDIICITGSFYLVGEAKIMLQNPVILAGQQPVQEPAMAPAMAAN"
     gene            complement(7712..8071)
                     /gene="folB"
                     /locus_tag="PLK08_00340"
     CDS             complement(7712..8071)
                     /gene="folB"
                     /locus_tag="PLK08_00340"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPS51980.1"
                     /translation="MIIEIENLRLRGIVGVYEHEQACPQDIIINAKLTFNAEQAVETD
                     DLEYAVDYEKLIGDIRSLLEHGRFRLLETVAQRITDMISADRRISHAEVKVSKPHAIS
                     AADRISVIASFDRLSQK"
     gene            complement(8079..8714)
                     /locus_tag="PLK08_00345"
     CDS             complement(8079..8714)
                     /locus_tag="PLK08_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPS51981.1"
                     /translation="MSQPVNIKTIPVGPLETNCHVIWNESDCFLFDAGWQSPKLVQFL
                     KDKSLVPSHVFCTHGHGDHIGGLKDIKANFPQIHICCPAGDIAMLSDPYLNFSASMML
                     NVVAPAADIVVKPGDIFKMGSLEWLVLDTSGHTAGGVSYYCRQADVVVGGDALFAGSV
                     GRCDLPGGSYDRLMENIHKNLLSLPDETVVLCGHGPQTTIGIERKSNPYLN"
     gene            9113..9730
                     /locus_tag="PLK08_00350"
     CDS             9113..9730
                     /locus_tag="PLK08_00350"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPS51982.1"
                     /translation="MMVKRLFDIIVSMAVLLLIWPLLLLIAVLIRLESHGHALFSQRR
                     AGYMGKPFTLYKFRTMFRDVEPYGNSPKSGDDPRLTRIGKFLRETSLDELPQLWNVFT
                     GSMSLVGPRPLYERQAEKWNERQKRRLNVRPGITGWAQIKGRGEIPIEEKIELDIYYL
                     DNWSLMLDLKIILYTFLQAIGKGQGIYEKRYSLDSYREADVMKKH"
     gene            9898..10929
                     /locus_tag="PLK08_00355"
     CDS             9898..10929
                     /locus_tag="PLK08_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPS51983.1"
                     /translation="MGSSVKKELNILFTCAGRRVVLIKRFRDAMQKLKLEGKFIAADI
                     SPLTAAFKFVDRWEIVPKARNVEYIPHLLSIVEKHRIGLIVPLTDLDLRSLARQADAF
                     KQIGCTVMVGDEPSVRICRDKQIFCQHVAAHGLPAIKTFTYEQFLKDPFFPCFVKPMH
                     GSAGIGATHINIMSRLVEHVKFYGDNMLVQSYVPGREYTIDVYKTRSGKIAAIVPRQR
                     LVVRGGEVEQGITVKNPVLIDAARKLVETLPGLWGCFCCQCRWPEGDDPYFFEINPRF
                     GGGVTLSIAAGANIPYYLIQDVLGMKVNGRVGHFTENLALMRYNEDIYRKIKSPDTLP
                     GFDQPVFHE"
     gene            10942..11637
                     /locus_tag="PLK08_00360"
     CDS             10942..11637
                     /locus_tag="PLK08_00360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPS51984.1"
                     /translation="MLSSYQLVAFDMDDTLYPERLFVKSGYDAVSELLQKRFSRQDNF
                     SDWMWQRFLAGKTSGAFNAVNEQFNLGLNEKDILELVEIYRLHLPDISPYDDMRPLLK
                     ELRRHFLLGVVTDGPAAMQRNKFARLGLGGFFHSVVFTGDLPEGSSKPSTAPFLKIAE
                     DLKVSNPSACVYVGDNPAKDFLGPNSLGWLTVQFHRPGQVHFHKPAPHGGLPQMTVSS
                     AKELFAVLTCEME"
     gene            complement(11644..12477)
                     /locus_tag="PLK08_00365"
     CDS             complement(11644..12477)
                     /locus_tag="PLK08_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPS51985.1"
                     /translation="MDVTDKNDDLIKVRQYGETGPFVILLHGGPAAPGYMAAVARELQ
                     DSFRVLEPFQRGSSEGNSLTVKKHVEDLYAVVKKYCGDEKPHIVGHSWGAMLALAYAA
                     DYPQSPRSLVMIGCGTFDKTARAKLAEIQNARMTDDFRRRLADLEKKYPDESVRIGVM
                     GKMFQRLYAYDLLPAREMSAFDVAQCDAAAHRQTWDDMVKQQEMGVYPVSFEKIVAPS
                     IMLHGDYDVHPGDMIFAGLKKHIPHLEYEHWEKCGHNPWLEQYARRDFFQTLKRWLNS
                     H"
     gene            complement(12529..14586)
                     /locus_tag="PLK08_00370"
     CDS             complement(12529..14586)
                     /locus_tag="PLK08_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HPS51986.1"
                     /translation="MNKGKNFFLMMLLALAAVFMFQSFFANKDKKQQPAETPTAAVSQ
                     PVAPQSEPVKDWFIKPAEQNIVALGSLTADKPYKMELRISATGACVESLKLANYFVTV
                     ADQRLYSKNPADYERLAAENPEKYKGHYQVLGNIDYGGHSYRSFETRQVNVQLEGQKL
                     PYVFTEMRDKPWQLVKISGADGKAVSFAWTFYKKAEPFGKKVPALTITKTYTVLEDGY
                     SVQMTLSLENNLKEPMTVAMEQAGPIGIQREDLRADMRMAVSAKLENDNIQPIHYGVK
                     ELSEWPYGQPHFIGSTTQLSDDKKESTPLVWFGMVNKYFGSMLYFNPDKNGLLAAVEY
                     DADYFISPVQASEFISGEENGRVWDTSMWVKDLKLAPGKIATLNFDIFAGPKLYSVFK
                     ENPLYARLHYSDTIITQGCAFMTFNWLMELLIWLLTFFATHLFFGNYGLAIILLVLIV
                     RVILHPLTKKGQVSMMKMQKQMARLKPELDKIKERCGNDKQRMQQETMKFYREHGNPM
                     TGMLGCLPMVLQMPIWVALYSGLNTAVSLRHAAFLPVWITDLAAPDQLVMWSRSLPLI
                     GDSFNLLPILLTVFMFLQQKYMTPPTSASMTPEQEQQQKMMKFMMPIMMLFIFYNMPS
                     GLNLYIMTSSAVGLVEQIYIRRHIKRQEELEAARETIIDIGGKGPRSNRPKKPKNPFG
                     IKF"
     gene            complement(14696..15466)
                     /locus_tag="PLK08_00375"
     CDS             complement(14696..15466)
                     /locus_tag="PLK08_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPS51987.1"
                     /translation="MLAIISDIHSNIEALAAVLDDIRRRGVRKTYCLGDIIGYGPDPG
                     ECLDMVQANTFRSILGNHDHAVLYGPEHFNSGAEGAIYWTRKRLEETADRQALQRRWE
                     YLASLDERVLINPPETGGLEMLLVHGSPRRPISEYVFPDDSRTSPTKMAGIFERFSHL
                     CFVGHTHLPGVFTEKDGFVPPADLGGVYEITDNKALINVGSVGQPRDRDPRASYVVVD
                     DKKVEFIRVPYDVNKTVLKVFNTEDLDNFLGSRLQEGR"
     gene            15932..>17686
                     /locus_tag="PLK08_00380"
     CDS             15932..>17686
                     /locus_tag="PLK08_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPS51988.1"
                     /translation="MLKTLHGYIAKDLVKITLLATFTFTLLMTVFAILEPLRKRGLAS
                     GQVFMLFGYTLPVMLALTLPFAALFATTIVYGRFAQDRELLACRASGIATGTTLRPAI
                     ALGVVVTIISMILTNFVSPEMARRGERNLVANLKQIAYHKLKKEGILSLGDRYIIHVS
                     RVREEDKADNIPAGLEGVIAGQRVKEMDPKTGKEFNSLKLVVASQAYLETTPDEETGK
                     HYASVQLNDQVGPVSYNFAGISSVDELPLTNIPIESPTKEKASFYSWPKLLATRKDPT
                     LNSEIGKDMRTFKRKIIQNRVMQTISKTINAGEPYAGLFSRPVVDDQTGKKYVEQYIV
                     SAGRAVVNGDTAKLESVTTKDGRIIPVVVRRIKGEMVKTTPDGKESFREAGPTTLIVA
                     NAGSIIARYEQTFDKTSVTISLSGNVMRKDSENPDAEMPVSASQKIGQILMPRDSELD
                     SKPISEFFQDWKAGRITRYTDDPEIIKQMQERLKKAPRKVQGEIKAEMNTRIAFGLSA
                     VLLVAMGAALGLMFKGGQMLSAFALSVIPAAIILVLIMMGKKMISNPGSSDTVGLIAI
                     WGGNAGMLLADVLVYLRIS"
BASE COUNT         4204 a         4406 c         4385 g         4691 t
ORIGIN      
        1 catgatgttt gagcttaaac ccgggcaggc cgtcgtgata tttccttttc agtaccacgc
       61 gtacgcgaat tttgcaaccg gaacgataga ctggctgttc acttcgtttg aatggcccaa
      121 cgcggttacg ccgtcggcgt tcgtgaaatt gaaaaacaca atcacgacca tgtcgccgga
      181 aatgctttca tttgtggaac ggctgataga gcattacacc ggcgagggcg acgaggatta
      241 tcgcgcaatg ggcgtaaagc tcatgaccgg tgcactgcta aacgaacttc tccttgccga
      301 ggaaacgcat cagaccatcc gcaatcgtgc cggcacgtca cacacgtatg aactgtttca
      361 ggtggtaagc cagtatattt atgccaacct cgatcagccg atacagatac gagacgtggc
      421 tgattacgcc aacatttccg aaagtcatct tcgcaatgtt tttacgcaga agttcggcgt
      481 gagtatcggc tcatacatca ggcgcagcaa actcattcgg gcgtgcaggt tgctggagac
      541 gacggaatcg tccatcggcg aagttgccca agcctgcgga tttggttcag tttatgcgtt
      601 cagccgtaca ttcaggcaga acatcggcca aagtccaaat caatatcgca atcgtccagg
      661 cagcagaaga aagcgccggc cggctagcac taatgtagaa taaagcggcg catgtaaaac
      721 atggcgtaaa ataaaatcaa ccaccactta aacacaactc ggatatattg caaagcgtac
      781 atgtcgtcat ttttatttaa aacagttctc gaagcggtat ttatcttttt actctggcgt
      841 tgccgttcca tatgctccag acctgttctt catccgccgg ctgagcgtaa tcagtaagaa
      901 gcgtcgtcgc cagtgctcct gtcgcccatc cgaattgagc ccatttttcc ggcgaccagc
      961 ctttaagaat ggcgtaaagc atgccgccca caaagccgtc gccgccgcct atgcggtcaa
     1021 gcacggttat ttcacgcggt tcgattacgt gccagtcgtt gccgtcgaac attatcgccc
     1081 cccagcgatg aagatttgca ctgacgactt cacgcaatgt cgtggcgaaa acgctggcgt
     1141 tgggaaattc ttttcttgcg ttatttatca tttccctgaa gccgtcgatt tttgcggcga
     1201 tgtccttgcc gccggcttcc gggcctttga cgcccaggca cagctgaaaa tcttcctcgt
     1261 tgccgattaa tatgtctgcc gccgccgcaa tttctccaaa aattttatgc aattcagctt
     1321 cgcgattgac ccagaacgaa gccctgtagt tgaggtcgaa agagattttc gttccgtatt
     1381 tttttgccgt ctgcgctatt tcgagacaga atttactggt ctccggcgac aaagctgcaa
     1441 tcaagccgga caaatgtact atctgtacgc cctcttcgca gaaaattctt tcgaggtcaa
     1501 aatcttttga gttgaggatt ctgccgactt cgccggcacg gtcattgtgt actcgcgggc
     1561 cgcgcgagcc tacgccacta tcggcaagat tgaactggtg acgatatccc cacgggccgc
     1621 cctgcggtac ttcagggcct tcgtaatcca tgtggcggcc ggcgaggttg tccttgataa
     1681 aacgggcgat aggactgccc ttgacgaaag ccgtcaaaac ttttaccggc aggccgagat
     1741 acgacgccac gctggcgacg ttggattcgg cacttgtcgc ctgcattttg aaaacatcgc
     1801 tgcaatgcat gggttggctg tctgccggcg tcaggcgtat gcccatactg gtgggtatca
     1861 acagcgagta tttgaaattc tttttaagtt cgatattcat atgcctgttt cctcatgttt
     1921 gattaagagc ttctaaacat atttgcacga attatttatg gccgccgttt ttcaaccgct
     1981 ctagtactct gtaatagagt atgctttaat tcttggctcg tttttgattc attcttccgg
     2041 cggccttacg tttggcaaca attctcccgg ctgaatcacc tgcaaaatga gaattcttta
     2101 ccaactttca agactctgct ttggagatat gttcatcggc atttatatca aacgccgcta
     2161 tatgcgctaa aaccgccgtc gaccggcact acaataccgg ttacaaattt tgcggcgtcc
     2221 gatatcagcc ataccagtgt gccgaccagg tccgccggtt cgccatatcg gctcatcggt
     2281 gtgtggtcga taatctgctg gccccgcctg gttttgctgc cgtcctggtt cgtcagcaga
     2341 aatctgttct ggtcggtcag gaaaaatcct ggggctatcg cgttgacgcg gatgttggga
     2401 gaatgattct ggcacatgta ggtgctcagc cattgagtga aattgcttac ggcggctttg
     2461 gcggcggagt aggctgctat tcgcgtcagc gggcgaaatg cattcatgga tgaaatattg
     2521 atgatgacgc cctgattgcg ttgggccatg tgtttgccga aaacttggct gggcactatg
     2581 ctgccgaggc agttcaggtc aaaaacccat cggattgcat cggcgtcgag gtcgaagaat
     2641 gttgattcgg gcggtacaca ggttgcttct ttcttgttgc cgccggcgcc gttgatgagt
     2701 acatctatat gttcgaaatt tttaactgtc tgttcaaatg cttcgacgac agccgatttg
     2761 tcaagcacat tgcatttgac ggctatcgca gttccgtcgg cggcgttgat tgcgtcagcc
     2821 actttttgtg cgccggcgag attgtagtcc agtattgcca ctctcgcccc gcaggccgcc
     2881 agcgattttg ccatctcgcc gcacagtatg ccggctccgc cggttatcgc aacagttttg
     2941 ccgttaacgt tgaacatatc gatactcatc ttgcacctca tcattttcgt agtcggtcgg
     3001 gcttgagttt accctcgaca taatcgttaa tatattgcag caattccctg cgactcggat
     3061 ttgcggggaa tttcaggttg tttttctgtg ctatcgtttc ccatctgttg acagcgtcgc
     3121 agaacatggg cgacggcatg aaatcccata ttacggtgtc cgtccaatcc ggttttttgg
     3181 tattcggcgg cgatacgggc atgggttcat accactgatc cagattttcc aaaattttac
     3241 gtcgaagttc attctgctcg gaaagcggca gggtgtctat ctcccttagc attgaaacgc
     3301 tccgccatac tttttcgcga tgctgttcca gaaattgaat tttctttgat aactgttcct
     3361 ttgttatggt tgcgtccggc agcggcgtca atttccgtat aggttttact tttccgcttt
     3421 cttccagttc gacgcgttca gcctctgtat ccggccggcc gtcggctatt tcttttttcc
     3481 cggattcgtc agccgtttcg tcgtcagcaa cggctggcct ttcccaccag ccccatttgg
     3541 gccagtcggg gtattgcttg cgaacatcca tattattcgt atcgcagccg ccaataattg
     3601 ccgccaatac agccatcaac atcatctgtt tcatgacaat tctcctgtgc aagactgaat
     3661 cacacaattt ttcagtgcat acgacgtagt tgtgtatatt ctgtcttctg gggctgaatt
     3721 gtcaaacaac aattggtacg ccacgataat ttaactgccg aaatacgcat tccgccggca
     3781 ggaatgcggt atatatcgca taatcacgcg ttgtacgtcg tagcggttat attgccgccg
     3841 tggccggtcc agttcgtgtg gaaaaattcg ccgcgcggcc ggtcgacgcg ttcgtaagta
     3901 tgcgcgccga aataatctcg ctgcgcctgc agaaggttag ccggcagcct tgcgctgcga
     3961 tagccgtcat aatatgcaag cgcactgctg aatgccggcg tcggtacgcc catatcgacg
     4021 gcaagcttga ccgtgtttcg ccatgcctgc tggcagtcgg cgactgcgct ggtgaaatag
     4081 ctgtccagca gaaggtttac cagattcgga tttttatcga aggcttcttt tattttgccc
     4141 aaaaaccggc tgcgaatgat gcagccgcct cgccacatca gggcgatgcc gccgtaattc
     4201 agattccagc cgtaagtctt agccgccgcc cgcatgagtt gataaccctg cgcatatgaa
     4261 acgattttgg atgcataaag agccttgctg atatctgcga caattcgggc tcgattgccc
     4321 tcgatttttt tgccggccgg ccctggcagg atttttgacg cttctacccg ctcttccctg
     4381 atagcggaaa ggcaacgtgc gaaaactgct tcgccgatga gtgtaagcgg ttggccttca
     4441 tctaatgcgg cgacggctgt ccatttgcca gtgcctttct ggccggcggt gtcaagtatg
     4501 ctgtcaagaa cttccctgcc ggcgtcatcc ctgtaaccga gaatgtctcg tgtgatttca
     4561 accagataac tgtcaaggtc gcctttgttc cagtcggcga gaacatggca catttcggcg
     4621 ttggtcagtc ccagagtttt catcaggttg tatgcttcgc agatgagctg catgtcgccg
     4681 tattctatgc cgttgtgaac catttttaca aaatggcctg cgccgccttc gcctacccag
     4741 tcgcagcatg gttcttccct gccggtcttc gggtcggggg cttttgccgc gatagcctga
     4801 aaaatctcct tcactgccgg ccatgccgcc ggactgccgc ctggcatcat gctcgggcct
     4861 ttgcgggcgc cctcttcgcc gccggatacg cccgtgccga tatagagcag acctttgctt
     4921 tccacgtatt tgcagcggcg gatggtgtcc ggaaaatgcg agttgccgcc gtctatgatt
     4981 atgtcgccgg tgtccagatg cggcaggaga agttctatga aatcgtcaac tggctggccg
     5041 gctttcacca gcatcatcac gcgacgagga cgttttaggc tggcaatgaa ttcttcgata
     5101 ctgtggcagc cgactatttt tgtgcccttc gcctgattgt tgagaaaatc gtccaccttg
     5161 ctgaccgtac gattgaagac ggcgatggtg aagcccttgt cgttcatatt gagcaccagg
     5221 ttctggccca tgacggctaa tcctataagg cccaaatcgg cttgtggctg tgacatgatt
     5281 gtttccttta aaaaccgtta aatttaaata aaatgaatcc ctaaagggct atgataattc
     5341 tcgaaacaaa tatttttccg gcaaatactc ttttatttta aaaaccgtag aattacgaat
     5401 gacatattag atcatattaa acttgacttt gcgtttgttg ctgtttgcgg cgtcggctgg
     5461 ctgcgtcagg ctgcatcgaa aaccatacaa ggtgcctgct tcgccttcct tgccaaccgt
     5521 gtcctcaacg acgcaacaag gcgcaatttc aaatttaaca tgctctaaaa aatccgccga
     5581 agtagcattg aatattgcgc catcgcgcag ctcttacgac agtttatgca tgataaccga
     5641 tatttcaagt gaagcggctt tttttgcact gaattgagag ccgtatttat tgaacaatag
     5701 tccaacagaa cgattctttc aagaaccacg aatgacagga aaaatttgaa tggtgctttt
     5761 tgcataacgt tttgagaaat ttctgccaga tggagttaaa tccggtgttg gtaattttta
     5821 aaaccgttct gccgatgcga gaggtttata tggtggctgt ctgtatgttg ccgccataaa
     5881 gcaataaaaa atgtcatagc cacaaaatat ggttatgaca ttttgttata tgcaaaacta
     5941 cgtctaatta gctgccattg ccggcgccat tgctggttct tgcacaggct gttggccggc
     6001 gaggattacc gggttctgca acataatctt cgcttcgccg acgagataga atgaaccggt
     6061 tatgcagatg atatcttccc tgctgacggc ggaatatgcg atttcaagcg cctgcttgag
     6121 gctgttcgtc acttgcgcta ttctgccgtc ggtcatttcg gcatagactt cagcaagctc
     6181 atgcgggtct gccgctcgag ggctggtgga agatttggtg aaaatcagct tgtcggcgcc
     6241 tgtcgctatc tgctccatca tgcccctgat gtccttatcg gcggcacagc cgaaaatcat
     6301 gaccatcgag tcatacggga tatgctggcc gatgccgcga atgagcgctt ttatggacgc
     6361 tgcgttatgc gcgccgtcaa caagtatttg cggttcagtt ttgatgattt ccattctgcc
     6421 tggaaggtag actctttcaa ggccatacag cgcctgcttg tcgtctattt tcatgccctt
     6481 ctgtttcagc acgtccagca aggctaacgc caggccgcag ttaatcgcct gatgttcacc
     6541 gagcaccggc acagccagat gctcgaactt gctgctgggt gtagtgaggc acacccgcgt
     6601 gtggcagcct tccgtgcggg atgattcgac gcgataactg aattcaattt catcgccggc
     6661 aaaaaacagc ggcgacttga cttcggaggc gacttttttc atcacacgtt tagcaatcgg
     6721 attctgcggg gcggagatga cgggaatttg cggcttgaat atgccggctt tttcacgggc
     6781 tatgtccgcc agcgtattgc cgagctgatg catgtgatcc atcgatatgc tcgtaatgcc
     6841 gcagacttcc ggctgaatga cgttcgtgct gtcaagccgg ccgccaaggc cagtctctat
     6901 cacggcgata tcgattttct tatcggcaaa atacatgaag gcgatagccg taaaaatctc
     6961 aaaaaacgtc ggacggtctt ttttcatctg ctcgacgtga gatgatattt ttgcgataag
     7021 tgcggaaagt tccggttttg caatgcattc gccgtttatg ctgattcgct cacgaacgtc
     7081 gcagatatgc ggcgacgtat acagcccgac cttgtagccg caatgctgaa gcatggaggc
     7141 aagcatcatg gaagtcgaac ctttaccttt cgtgccggcg atgtgaactg attttatctt
     7201 tttgtgagga ttgccgattt tcttcagcag tttttccatt ctgtcaaggc tgaacgtatc
     7261 cctgttgtat cttacgcgca gcatttgttc ataatcggtc aacgagaaca gatatttcaa
     7321 tgcttgcggg tagttccgga attttacgcc actcgccgcc gttttaacgg ctttttccgc
     7381 ccgcgtggtc gttttctttt tcgtaccctt attagtcttt cgacttgcta ttgccaccat
     7441 tactttatcc tgttttaaaa aaacggcttc taaatattga cgcatcacac acaaacctgc
     7501 gcgccattac ttacggattc atgtgtgacg aacataaatt attctgctac ccgcatattc
     7561 aaagggtgaa aataattaag atagattatt gtatcaaatt tgaccagaaa agtcaagaaa
     7621 atcgacttaa cgccatgatt acaatggagt taaaatcgca ccgcaaccgg ctgacaggca
     7681 tgaattcaac aattatttat tgaaaaatcg tttatttttg cgacagcctg tcaaagctgg
     7741 cgattacgct tatgcggtct gccgctgaaa ttgcatgggg cttcgagact ttgacttcgg
     7801 cgtgcgatat tcgtctgtcg gcggatatca tgtctgttat acgttgcgca acggtctcaa
     7861 gcagacgaaa tctgccgtgt tccaagagag aacggatatc gcctatgagt ttttcataat
     7921 ccacggcata ttccaggtcg tccgtttcaa ccgcctgctc cgcattgaat gtgagttttg
     7981 cgtttattat gatatcctgc ggacaggcct gctcatgttc gtaaaccccc actatgccac
     8041 gcagtcgcag attttcaatt tctattatca tggctgaatc aattcaggta cggattgctt
     8101 ttgcgttcaa tgcctattgt agtttgcggc ccatgtccac ataataccac cgtttcgtca
     8161 gggagggaaa gcagattttt gtggatattt tccatcagcc ggtcataact gccgcccggc
     8221 agatcgcatc tgccgacact gccggcgaac agtgcgtcgc ccccgactac aacgtcagcc
     8281 tgccggcagt aataactcac tccgccggcg gtatggcctg atgtatcgag aaccagccat
     8341 tcaaggctgc ccattttgaa gatatcgccg ggcttgacga cgatgtcggc agctggcgca
     8401 acgacgttca gcatcatcga tgcggaaaaa ttcagatacg gatcgctaag catggcgatg
     8461 tcgccggctg gacagcagat atgaatttgc ggaaaattgg ctttgatatc cttcaagccg
     8521 ccgatatggt caccgtggcc gtgggtgcag aatacgtgcg acggcacaag acttttatcc
     8581 ttgagaaatt gaaccaattt cggcgactgc cagccggcgt cgaacagaaa acaatccgat
     8641 tcgttccaga tgacatgaca gttcgtttcc agggggccga ccggaattgt ttttatatta
     8701 acgggttgcg acatttctat ctccggaatt ttttacagca ttctgccgca agctacaata
     8761 aaaaatgtcg gagttccgat ttcatggctg caggcacgaa tgattgcaag tctgtgcgga
     8821 aacggcagaa ttttgtaaac aactgcgggc aggatagcag aaaaccagcc atttttcacc
     8881 tgttttttat tgaattaacc ttaattcgat gttaaactta ttgtgcttgt aagatagcct
     8941 gtccggcgaa taaagcttgg ccggcggcga aagagctttt taaaattttt ttaagctctt
     9001 gatacggagt gtgcagattc tgtgaaaaag tttttgtgtg agccttcttt tattttgagg
     9061 gattcatctc atttatcatt ttccggaatc caggcaggag tgtggatttc caatgatggt
     9121 caagaggctg tttgatatca ttgtcagcat ggctgtattg ctgttgattt ggccactgct
     9181 gttattgatt gcagtcctga tacgtctgga atcgcacggc catgccctgt tcagtcaacg
     9241 ccgcgccggc tatatgggca agccctttac actttataag ttcagaacga tgtttcgtga
     9301 cgtcgagcct tatggaaatt ctcccaaaag cggagacgat ccgcgactta cccgaatagg
     9361 aaaatttctc cgcgaaacaa gtctcgacga actgccgcaa ttatggaacg tttttaccgg
     9421 cagcatgtcg cttgtcgggc cacggccgct gtatgagcgg caggctgaaa aatggaacga
     9481 acggcagaaa cggcggctga acgtccggcc gggcattacc ggctgggcac agattaaagg
     9541 cagaggcgag atacccatag aagaaaaaat cgaactggat atttattatc tggataattg
     9601 gtcgctaatg ctggatttga aaataatttt atatactttt ttgcaggcga taggaaaagg
     9661 acagggcatt tacgaaaaac gttacagtct cgattcgtac agagaagctg acgtgatgaa
     9721 aaaacattag agcatgttaa acttgatatg ctctaatggc gaacggcgaa ggaattaaat
     9781 tcgtagccgt tcacggatta tcggacagcc cgacattcgg cggatgtgta tatctgcaat
     9841 ttttgcagag ccgccgaaat caagtttttt aatatggtat gtcctggtga aaatgcgatg
     9901 ggtagtagtg taaaaaaaga gttaaatatt ctgtttacct gtgcaggccg acgggtcgtt
     9961 cttataaaac gatttcgcga cgcgatgcag aaattgaaac tcgaaggcaa attcattgca
    10021 gccgatattt cacctcttac cgcagccttc aaattcgtcg atcgctggga aatagtgccc
    10081 aaagcccgca acgtggaata tattccgcat cttctttcga tagtcgaaaa acaccgtata
    10141 ggcctgatcg tcccgctgac ggatttggat ttgcgaagcc ttgcccggca ggcggatgct
    10201 tttaaacaaa tcggctgtac cgtcatggtc ggcgacgagc cgtccgtacg aatctgtcgt
    10261 gacaagcaga ttttctgtca gcatgttgcc gctcacggct tgccggctat aaaaactttc
    10321 acctatgagc agtttctgaa agaccccttt ttcccgtgtt tcgtaaaacc catgcatggt
    10381 tcggctggca taggcgcgac gcatataaat atcatgagcc ggttggtcga gcatgtaaag
    10441 ttttacggcg ataacatgct tgttcaatcg tatgtgccgg gccgtgaata caccatagac
    10501 gtatataaaa cccgctccgg aaaaatagcg gctatcgttc cgcgtcagcg tctcgtggtg
    10561 cgcggcggag aggtcgagca gggcattaca gtaaaaaatc ctgtccttat cgatgccgcc
    10621 agaaaacttg tggaaacgct acccggcctt tggggatgct tctgctgtca gtgtcgctgg
    10681 ccggaaggcg acgacccgta tttctttgaa ataaatccca gattcggcgg cggcgttacg
    10741 ctaagcatcg ctgctggtgc gaatattcca tattacctca tccaggacgt tctgggaatg
    10801 aaggtcaacg ggcgagtcgg ccattttacg gaaaatctgg cgcttatgcg atataacgaa
    10861 gacatctacc gcaaaatcaa atcgcctgac accctgccag ggtttgacca gccggtattt
    10921 cacgaatgac aggtattggc catgttatct tcgtatcaac tagtcgcgtt tgatatggac
    10981 gacacacttt atccggaacg gctgtttgtc aaaagcggtt acgacgccgt atccgaactg
    11041 ctgcaaaaac gtttcagccg gcaggataat ttcagtgact ggatgtggca gagatttttg
    11101 gctggtaaga catccggtgc attcaatgct gtaaacgagc aatttaatct gggattgaac
    11161 gaaaaagata ttcttgaact cgtcgaaatt tatcggcttc atctgccgga catatcgccg
    11221 tatgatgaca tgcggccgct tctgaaagaa ttgcgacgac attttctgct cggcgtagtg
    11281 acggatgggc cggctgcgat gcagcgaaac aaattcgcaa gattgggcct gggcggtttt
    11341 ttccattccg tggtatttac cggcgatttg cctgaaggtt catccaaacc gagtacggct
    11401 ccgtttttga aaattgcgga agatttgaaa gtgagcaatc cgtcagcctg cgtttacgtc
    11461 ggcgacaatc cggcaaagga ttttctgggg cccaactcgc tcggctggct gaccgtacag
    11521 tttcaccggc ccggccaggt tcattttcac aagccggctc cacatggagg cctgccgcag
    11581 atgaccgtca gttccgcaaa agaacttttt gcggttctca cttgtgaaat ggaataatct
    11641 ttttcagtga gaattcagcc accgtttgag agtttgaaaa aaatcccttc tggcgtattg
    11701 ttcaagccac ggattatggc cgcatttttc ccaatgctca tattcgagat gaggaatatg
    11761 tttcttaagg ccggcgaaaa tcatatctcc aggatgaacg tcatagtcgc catgcaacat
    11821 tattgacggc gcgacgattt tttcaaaact gaccggatag acgcccatct cctgctgttt
    11881 caccatatca tcccaggtct gccggtgagc cgccgcatcg cattgtgcga cgtcgaaagc
    11941 cgacatctct ctagccggca gcaggtcata tgcataaaga cgttgaaaca tttttcccat
    12001 cacgccgatt cgcacggatt catcgggata ttttttttcc aggtcagcca gccgccgtct
    12061 gaaatcatca gtcatcctgg cgttctggat ttccgcgagt tttgcgcggg cggttttgtc
    12121 gaatgtgccg cagcctatca ttaccagcga ccgcggcgac tgtggataat ccgccgcata
    12181 cgcaagcgcc agcatcgctc cccatgaatg gccgacgatg tgaggctttt catcgccgca
    12241 atattttttt acgaccgcat acaggtcttc gacgtgtttt tttaccgtaa gcgaattgcc
    12301 ttcgctgctg ccgcgctgaa acggctcaag cacgcgaaaa gaatcctgta attcacgcgc
    12361 gaccgcagcc atataacctg gagcggctgg cccgccgtgc agcaatatga caaacggacc
    12421 cgtctcgcca tattgccgga cttttatcag gtcgtcattt ttatccgtta cgtccattat
    12481 tcaaagacat tctgtccgct tatatgcggc cgtggttcag tcaaatcttc agaattttat
    12541 tccgaacgga ttttttggtt ttttcggccg gtttgagcga gggccttttc cgccgatatc
    12601 tataatcgtt tcacgggcgg cttcaagttc ttcctgacgc ttgatatgcc ggcggatgta
    12661 tatctgctca acgaggccta cggctgatga ggtcatgata taaaggttca atcctgaggg
    12721 catattgtag aaaatgaaca gcatcattat cggcatcatg aatttcatca tcttctgctg
    12781 ctgttcctgt tcaggcgtca ttgatgccga cgtcggcggc gtcatatatt tctgctggag
    12841 gaacatgaac accgtcagaa gaatcgggag caggttgaaa ctgtcgccga taagcggcag
    12901 cgagcggctc cacattacca gctggtcagg tgcggcgagg tcggttatcc agacgggcaa
    12961 aaatgcggcg tgacggaggc tgacggcggt attgaggccg gaatacaacg ccacccatat
    13021 cggcatctgt agcaccatcg gcagacagcc gagcatgccg gtcatgggat tgccgtgttc
    13081 gcgataaaac ttcatggtct cctgctgcat tctctgtttg tcgttgccgc agcgttcctt
    13141 tatcttgtcg agttccggct tgagtcgggc catctgcttc tgcatcttca tcatggatac
    13201 ctgacctttc ttggtcagcg gatgcagaat gacgcgaacg ataagcacca acaggataat
    13261 cgccaagccg taattgccaa agaacaggtg cgtcgcaaaa aacgtcaaca gccatatgag
    13321 cagctccatc agccagttga acgtcatgaa cgcacagccc tgcgttataa tcgtgtcgga
    13381 gtagtgcagc ctggcgtaaa ggggattttc cttgaatacg gaataaagtt tcgggccggc
    13441 gaagatatcg aaattcagcg tggcgatttt gcccggcgca agtttcaaat cctttaccca
    13501 catggacgta tcccataccc tgccgttttc ctcgccggaa atgaattccg acgcctgaac
    13561 cggcgatatg aaataatcgg cgtcatattc cacggccgcc agcaggccgt ttttgtcggg
    13621 attaaaataa agcatcgagc cgaaatattt gttcaccatg ccgaaccaga caagcggagt
    13681 cgattctttt ttgtcgtcgg aaagctgggt cgtcgagccg atgaaatgcg gctggccgta
    13741 cggccattca ctcagttcct ttacgccata gtgtatcggc tggatattgt cattttccag
    13801 tttggcggag acggccattc tcatgtcggc acgcaagtcc tcgcgttgaa tgcctatcgg
    13861 gccggcctgt tccatcgcca ccgtcatggg ctctttcaga ttgttttcga gcgaaagtgt
    13921 catctgcacg gaataaccgt cttccagcac ggtgtacgtt tttgttatgg tcagtgccgg
    13981 aacttttttg ccgaaaggtt ctgctttttt ataaaaagtc caggcgaatg agacggcttt
    14041 cccgtccgca ccggaaattt tcacaagctg ccaaggtttg tctcgcattt ctgtgaacac
    14101 gtagggcaat ttctggcctt caagctggac gttcacctga cgagtctcaa acgaacgata
    14161 agaatggccg ccgtaatcga tgttacccaa tacctggtaa tggcctttgt atttttccgg
    14221 attttcagcg gcgagccgtt catagtcggc tggatttttc gagtaaagcc gctggtcagc
    14281 cactgttaca aaataattcg ccagcttcag ggattccacg caagcgcctg tggcggatat
    14341 tcgcaactcc atcttatatg gtttgtctgc cgtcaatgaa cccagcgcga caatattctg
    14401 ttccgccggt tttatgaacc agtctttcac gggttcggat tgcggcgcaa caggttgcga
    14461 aacggcagct gtcggggttt cggctggctg ttgtttcttg tccttgttcg caaaaaagga
    14521 ctggaacatg aatacggcgg caagcgccag caacatcatc agaaaaaaat tctttccctt
    14581 gttcaattta aaaatctccg aacaatattt atcaggcttt cgtatgtctg ccagataacg
    14641 gttatttcat atcatggttt caaaatcgca aaattacgag aggacgtatt aaaaattatc
    14701 gtccttcctg aaggcgcgaa ccgaggaagt tgtccaggtc ttccgtatta aacactttca
    14761 gcacggtttt attcacgtca taaggaactc tgatgaattc aactttttta tcatctacga
    14821 cgacataact tgcgcgtggg tctctgtctc tgggctggcc caccgagccg acgtttataa
    14881 gggctttatt atcggttatt tcataaaccc cgcccaaatc cgcaggcgga acaaagccgt
    14941 ctttttccgt aaacactcca ggcaaatgtg tgtggccgac aaaacacaaa tgcgaaaatc
    15001 gttcaaaaat tcccgccatc ttggtgggac tggttctgga gtcgtccgga aaaacgtact
    15061 cgcttatagg ccttcgcggg ctgccgtgca ccagcagcat ctccaagccg cccgtttccg
    15121 gcggatttat caacactcgt tcgtccagcg aagccagata ttcccagcgg cgttgaagtg
    15181 cctgccggtc agccgtttct tccagccgtt tcctggtcca gtaaattgca ccctcagctc
    15241 cggagttgaa atgttccggg ccgtaaagca ccgcatgatc gtgatttccg agtatcgacc
    15301 tgaaagtgtt agcctgtacc atatcgagac actcgccagg gtcggggccg taacctatta
    15361 tgtcgccgag gcagtaagtc tttctgacgc cgcgacggcg gatgtcgtcc agcacagcgg
    15421 cgagggcttc tatatttgaa tgtatgtcgc taattatcgc tagcattgct ttacctgtcc
    15481 atacagtcag gccatgatat gaaaaaaaat gcccgaaaac aattttttaa tgaaaattgc
    15541 cggctgtaaa aatttttgat atgtcatccg tagttattga agatgccaat ccgcagaaaa
    15601 atactttttt agagaattgc gctcactata atcccaaaaa tattcaagac ggggtttgtc
    15661 agggcaggcc ggcagtattg tattttttga atgaaaaatc gggacgaaaa ggttaaagta
    15721 ttcgcgtgtg gaagtctgcg ctcattccgg cggcaggcat gatttgtttt ttggaatcgg
    15781 cagcgttttg cggctagccc gaaatttagc cgtctgactt gccgccagtc aaaaaatacc
    15841 tccacgttgt agtccatttt ttgaagacgt ttggaaaagt tttgaacttc agaaattttg
    15901 ggaaatttaa aaaaagtcag gctcagtata catgctgaaa acattacacg gatatatcgc
    15961 caaagacctg gtcaaaatca cgttactggc gacatttaca ttcacgctgt tgatgacggt
    16021 cttcgccatc cttgagccgt tgcgcaaacg cggcctggca tcgggccagg tattcatgct
    16081 gttcggttat acgctgccgg tcatgcttgc cctgacgttg ccgtttgcgg cattgtttgc
    16141 aaccacgata gtttacggcc gttttgccca ggatcgcgaa ctgctggcct gccgggctag
    16201 cggtatcgcc accggtacaa cactccggcc ggctatcgca ctgggcgtcg tggtgacgat
    16261 tatttccatg atattgacga atttcgtttc gcctgagatg gctcgtcgcg gggaacgcaa
    16321 cctggtggct aacctcaagc agattgccta tcataaactc aaaaaggaag gcatcctgag
    16381 cctgggcgac agatacataa ttcacgtttc ccgcgtccgc gaagaagaca aggcggataa
    16441 cattcccgcc ggccttgaag gcgtcatcgc cggccaacgc gtaaaggaaa tggacccgaa
    16501 aaccggtaag gaattcaatt cgctaaaact tgtcgtagca tcgcaggcgt atctggagac
    16561 cacgccggac gaggaaaccg gcaaacatta tgcgtcggtt cagctcaatg accaggtcgg
    16621 gccggtgtcg tataactttg ccggcatctc ttcagtagac gaactgccgc tgaccaatat
    16681 cccgatagaa tcgccgacga aggaaaaagc cagcttctat tcctggccca aactgctggc
    16741 tacccgcaaa gaccccacgc tcaacagcga aatcggaaaa gacatgcgca cttttaagcg
    16801 taaaataatc cagaacaggg tcatgcagac catatccaag accatcaacg ccggcgaacc
    16861 atatgccggc ctcttcagca gacctgtagt ggatgaccag accggaaaaa aatatgttga
    16921 acaatatatc gtttctgctg gcagagctgt agtgaacggc gataccgcca aactggaaag
    16981 cgtaactaca aaagacggca gaataattcc cgtcgtcgta cgccgcatca agggtgaaat
    17041 ggtaaaaaca acccccgacg gcaaggaatc tttcagggaa gccgggccga ctacgctgat
    17101 agtggcaaac gccgggtcaa taatcgccag atatgagcag acgttcgaca aaacgtcagt
    17161 cacgatatcg ctcagcggca acgtgatgcg caaggattcg gaaaatcccg atgcggaaat
    17221 gccggtgtcc gcctcgcaga aaatcggcca gatactcatg cccagggaca gcgaacttga
    17281 ctcaaaaccc ataagcgaat ttttccagga ctggaaagcc ggccgaataa ccagatatac
    17341 agatgatccg gaaattatca aacagatgca ggaacgcctg aaaaaagctc cgcgaaaggt
    17401 tcagggcgaa atcaaagccg aaatgaacac ccgcatagcc ttcggcctca gtgccgtgct
    17461 gctggttgcg atgggcgctg cgttgggcct gatgttcaag ggcggccaga tgctcagcgc
    17521 atttgcgctt tcagtcatac cggcggcgat tatcctggtt cttataatga tgggcaagaa
    17581 aatgatatcc aatccgggtt cttccgacac ggtaggactg attgcaatat ggggcggcaa
    17641 tgctggcatg ctgctggcgg acgtgctggt ttatctcagg ataagc
//
LOCUS       DAONFI010000005        16738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_3642_length_16738_cov_4.512618, whole genome shotgun sequence.
ACCESSION   DAONFI010000005 DAONFI010000000
VERSION     DAONFI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16738
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3642_length_16738_cov_4.512618"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLK08_00385"
     CDS             complement(<1..663)
                     /locus_tag="PLK08_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPS51989.1"
                     /translation="MQETASTSRPESKPQGEREIVILGAREHNLRNIDVRIPRDRLVV
                     ITGLSGSGKSSLAFDTIYAEGQRKYVESLSAYARQFLEQLSKPDVEHIEGLPPTVAIE
                     QRAGSANPRSTVATTTEIYDYLRLLFARVGTPHCWICGREITSQTVTQMVDAVMSLPD
                     GTKFMILAPIIRGQKGQHAELLAHISSEGYVRVRVDGEICDIKAMPELAKNKVHTIEV
                     VVD"
     gene            848..2032
                     /locus_tag="PLK08_00390"
     CDS             848..2032
                     /locus_tag="PLK08_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51990.1"
                     /translation="MKIRFFKITVLVAVLTFSCFALGQAGEMRLQPAIDLPGIGLRMA
                     VPLSYVYTDPDEPFKAYSCVLTDRGQAVQNITLMAYPYDKSQTVKDFAASTLTDIKNN
                     LAIRELKVLSETPIQVAGLAGIARAMEYNFRGIDTSSVTLIFTRSLEQFKAANPLCAD
                     QIIYVLSMEVSIEQKESLLAQLDMVVRSISLMPLKSPTDMPVNLRHRVFIKNFSCGLA
                     IRLPEFWWSEITGDGIKMGRYDYLCNDDSPMLEIVSVLVSDSLTAQACGQAAIDFDKK
                     RGLSFDILQQGPLKIAGKLGYQFILRQSETQAAESQPAGTKKTAETKDYVIQIRRLLC
                     VPDSTAGKNRHYAMILTGFSADVKKLCDIMDELTDSFDTLPVVKEMPKAPANPASDRE
                     GL"
     gene            2036..3076
                     /locus_tag="PLK08_00395"
     CDS             2036..3076
                     /locus_tag="PLK08_00395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPS51991.1"
                     /translation="MTKVGMNVIEQGDCIKILDSVEEPFADLVFADPPFNIGYKYDVY
                     EDKLAYEEYYDWTEKWMAACTRVMKPDASFWIAIGAEYAAEVRLIGRKLGLTLRNWII
                     WHYTFGQNTKKKFARSHTHLFYFVKDSGRFTFNDKAVRVFSDREVYYHDKRANPQGRI
                     PDDVWDEFPRLCGSFSEREGWHPCQMPESLLARIIRTCSNVGDVVIDPFAGSGTTLVA
                     AKKLSRRYFGTEISPQYVEGVRQRLNKTRAYAGGCPEKSQPADCSDDKIPPAHFEILR
                     DAYADFTVPVTKLKTSGFLMERFAERYNLRLKYSGVACGYTAQAILGCLEKMSKKIGA
                     LPVIKCHSIDGK"
     gene            3086..3862
                     /gene="xth"
                     /locus_tag="PLK08_00400"
     CDS             3086..3862
                     /gene="xth"
                     /locus_tag="PLK08_00400"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPS51992.1"
                     /translation="MKISIATWNVNSVRPRMERIAEFLRRVPADVLCIQELKCRTEDF
                     PYDAFDELGYNCAVNGQKTYNGVAVVSRLPIDDIRCGLGDTELDEQARLISAEIAGLR
                     IYSAYVPNGSDMESEKYRFKLRWLTRLSEILEESHSADEPVVICGDTNVIINDDDAND
                     IVPWRTTGLACDEVRLALQKVTDLGLVDVFRKINPEGGKFSWWDYRQLAFVHNIGLRL
                     DHILASRPLAEKLCSCVIDRESRKGQKPSDHAAVIAEFNI"
     gene            complement(4186..7128)
                     /locus_tag="PLK08_00405"
     CDS             complement(4186..7128)
                     /locus_tag="PLK08_00405"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPS51993.1"
                     /translation="MSNELAKVYDPRAVENEAYEKWQKHNSFAAAANDPRESFCMVIP
                     PPNVTAALHLGHALNNTLQDVIIRFKRMSGFATLWMPGTDHAGIATQTVVEKRVLAEE
                     GKRRTDFKREDFVAKIQAWKDEYEATILSQLKLMGCSCDWARTRFTMDSVCAKAVRET
                     FSRLFSDGLIYRGKRLVNWDPATQTVLADDEVEHEDIAGFFWYLKYPLEKPAEINGKK
                     ITHVTVATTRPETMLGDTAVAMNPADPRAKVLAGTMIRLPIVGRLIPIILDDHVVLPD
                     PESSDEKARFSTGFLKVTPAHDPDDYQIGQRHSLAMINIMAPDGSISDRHGWDDCNTE
                     EAKNLLGMDRFEAREAIVEWFRNENLLEKVLDYTHSVGHSYRSHVPIEPYLSDQWYVA
                     VKKPIENFKLRIANCKSEERDGVKYIPDTDVPLNSLAGLALQPLLDGRLKFTPARYAN
                     NYQAWLENLRDWPISRQLWWGHQIPVWSKAGEHPNRCKYGTTCQVFQTDNGPVTFLCV
                     KPGFDELEKAIESEGWQRDADVLDTWFSSALWPFSTLKWPSDDPADKALLEKFYPGNV
                     LSTAREIITLWVSRMVMMGQYCAGDIPFSEVFIHAMIQDGQGRKMSKSLGNGVNPLDI
                     IDSHGADAMRYTLVSMTTQTQDVRMPIEKMTLPDGREVNSSPKFDIGRNLCNKLCNAS
                     RFAIMNLSDAPQWSDTDTSANLADRWILSRMTSVVMDAADAIEKYRFNDLAEKLYHFL
                     WDDFCDWYLEVAKARINAGEAAPKAVTAYVLDTILRLMHPIMPFITEAIWANLNAVVP
                     SRGPAGIAPEMLITAPWPTADESLVDADSESAFATLADAVRQIRNARQQHNVAPKERV
                     NIIIESAGENDVLQSNIELLEKLAGLGEATVTKQIATPPAGSAAISIGGRKIFVLDII
                     DTAAELERLGKLRATLEKGIAGVTAKLGNANFISRAPAEVVENEKQRLAEMQANLRTI
                     VESINAMK"
     gene            complement(7392..8984)
                     /gene="cimA"
                     /locus_tag="PLK08_00410"
     CDS             complement(7392..8984)
                     /gene="cimA"
                     /locus_tag="PLK08_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPS51994.1"
                     /translation="MKTGEKVELYDTTLRDGTQAKGVSLSLQDKLTAARMFDELGVDY
                     IEGGYPLSNPKDEQFFQEMRKIRLANARLAAFGMTRRKATKSADDAGMNALLHSEAPV
                     VTIVGKTWDMHVKEVLGVSKQENIDMIAESVAFLAAAGRFVIFDAEHFFDGYRANMEY
                     ALKTLAAAMDAGAQRLVLCDTNGGTMPEYISSVVGTVLNALPAAKLGIHCHNDCDMAV
                     ANSLAAVNAGIIQVQGTINGVGERCGNADLVSVSANLALKYGRKVLTEGAIAKLTEVS
                     RGFYELSNMNFRDNQPYVGSNAFAHKGGMHVHAVQKISCSYEHVTPETVGNTRRILVS
                     ELAGVSNIAATVPAKFKLDKDRDAQKKILARVVELENEGYEFERAEASFEMVIRETLG
                     GKWYTPLWKLDHYRCIILKRDSEKATTEATVKLAFDDGQTMHTVREGDGPVDSLAGAL
                     KDALREKYHGLDRLHLMDYRVCVVNTAAETAAKVRVVIDWRSGDKYFGSVGVGENIID
                     ASWRAMVDAIEFQLLNDREAAK"
     gene            9673..11955
                     /locus_tag="PLK08_00415"
     CDS             9673..11955
                     /locus_tag="PLK08_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS51995.1"
                     /translation="MPRQCGIATFTHDLCEAVAAEVADKTPVFAVAMNDRPDGYAYPH
                     RVHFEIRQNSQNDYRLAAEYLNMQQPAAICLQHEYGIFGGPYGSHILSLLRRLRRPLI
                     TTLHTVLESPSEQQKLILQEITRLSTRVVVMAEMGKKFLEDVFHVPAEKIAVIPHGIP
                     DVPFVDPHFFKDQFGVEGKKVMLTFGLLSPGKGLEYAIEAMPAIVKKHPELVYIILGA
                     THPHVKAEYGEEYRNGLTRRVNELGVRDNVIFVNRFVVLKELCEYLGAAELYVTPYLN
                     EAQMTSGTLAYAMGTGKAIVSTPYWHAREVLADGRGTLVPFRNSQAVADAVNNLLDNE
                     NELNAMRKNGYNYCRRMIWPCVAESYLNLFTQAGEAWQEDQRTAKRHGDIKPRRREKF
                     DLPEIDLKHMQLMTDDTGIIQHCVYITPDRYEGYCVDDNARALIVAGMNWQLLKDESV
                     IPMMNTYLSFLYYSQDKKSGRFRNFMNYDRTWRDEKPSEDAHGRAMWALGEAVANCPH
                     ESMIALASRMFQVGLGSVKSFTSPRAWAFTLCGIHAYLRRFSGDSRIRRHRAALSQKL
                     FDLFRLNVTEDWRWCEDIVAYASAKLPHVLMMSGRWMQRGDMVDLGRRALGWLIDIQT
                     NDDGFFSFIGSDGWYRRGHKKAHYAQQPIEAHAMLDACIEAFHITHENYWIDQAQKAF
                     YWFLGENDLRRNLYDFTTGGCRDGLQADGVNENQGAESTLAWLMSLQQMHALQEELSL
                     AEIAAEQNDDSPNGLAVKTE"
     gene            12052..13275
                     /locus_tag="PLK08_00420"
     CDS             12052..13275
                     /locus_tag="PLK08_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS51996.1"
                     /translation="MSDPIVMISSYPPRLCGIATFCEEAREFIKKANPDRDVLVISHT
                     DGKGDGVFPIIDLSSRLWWKPVAEKIHQLKPHVVHIEHEYGLYNFLDARGISDGNRGL
                     LNLLEAISDVPIVIEPHTVHGRLTDFEAEFIYQMAARVDVCLFKCHYQKWRLDWSFSG
                     LGWPVPENIMVVPHGARADRRYAVSEVPELRKELNLDRTKLSNHVVGLIGWIQSNKRW
                     DILMEMWEEVAAEIKQRTNQDWDLLAAGMLRDPHHKADYEKYRSEVEILERKGLARYY
                     EFVPRGEIYYKMMAICDFIVLPSTDETQSGTLARIIALNKPYITTAPMEGLTAQTLES
                     GGGLLFTNREMLKRQVIALACSRKRRMQLGDNLRNYLDNVVSWEIVAGQYNEAYELAR
                     NAKKTGVKVKLPKEF"
     gene            13526..13599
                     /locus_tag="PLK08_00425"
     tRNA            13526..13599
                     /locus_tag="PLK08_00425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13560..13562,aa:Ile,seq:gat)
     gene            13646..13718
                     /locus_tag="PLK08_00430"
     tRNA            13646..13718
                     /locus_tag="PLK08_00430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13679..13681,aa:Ala,seq:tgc)
     gene            14150..>16738
                     /locus_tag="PLK08_00435"
     rRNA            14150..>16738
                     /locus_tag="PLK08_00435"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3872 a         4036 c         4630 g         4200 t
ORIGIN      
        1 gtcaaccacg acttctatgg tgtgaacctt gttcttagca agttccggca tggctttgat
       61 gtcacagatt tcgccgtcca cgcgcacccg cacgtagcct tcactgctga tatgagcgag
      121 aagttcggcg tgttggcctt tctggccgcg gatgatgggg gctaatatca tgaatttcgt
      181 gccgtcgggc aggctcatga cggcgtcgac catttgcgtt acggtctggc tggtaatttc
      241 cctgccgcat atccagcagt gcggcgtgcc gacgcgggcg aagagcagcc gaagataatc
      301 gtaaatttcg gtcgtcgtgg cgacggtgga gcgggggttt gcactgccgg ccctctgttc
      361 gatggctacc gtgggcggca ggccttcgat atgttctacg tcgggtttgg agagctgttc
      421 gaggaactgg cgggcatagg ctgagaggct ttcgacgtat ttacgttggc cttcggcgta
      481 gatggtgtcg aacgccagtg aacttttgcc cgatccgctc aggccggtta tcaccaccag
      541 ccggtcgcgg gggattcgca cgtctatgtt gcgcaggttg tgctcgcgag cgccgagaat
      601 gactatttcc cgttcgccct ggggtttact ttcaggtcgt gatgtactgg ctgtttcctg
      661 catcttgaat ataaacgttc agtgtactaa atttggatgt gaaaaacaat acccaaacgc
      721 aagtcagacg gcggctggaa tatatctatt gcgttgcagt aagccggctg gccaagtata
      781 atttcttcgt ctgtacgaat cagatagatg tacggacaga gatgcggctt ttccgtgagg
      841 acgataaatg aaaatcagat tttttaaaat aactgtgctg gttgcggttc tgacgttttc
      901 ttgcttcgca ttgggccagg ctggcgagat gcgcctgcag cctgcgattg acctgcctgg
      961 catcggatta cgcatggctg tgccgctttc ttacgtgtat accgaccctg acgagccgtt
     1021 caaggcatat tcgtgcgtat tgactgaccg cggccaggct gttcagaaca taacgctaat
     1081 ggcgtatcct tatgacaagt cgcagacggt caaagatttt gcggcttcga ccctgactga
     1141 catcaaaaac aatctggcga tacgcgaact gaaggtgctc agcgaaacgc cgatacaggt
     1201 ggcggggtta gctggcattg caagggctat ggaatacaat ttccgcggca tagatacgtc
     1261 gtcggtgacg ctgattttca cccgttcgct tgaacagttc aaagccgcca atccgctttg
     1321 tgccgaccag ataatttacg ttctgtcgat ggaagtatcc atagagcaga aggaatccct
     1381 gctggcacag cttgacatgg tagtcagatc catcagcctc atgccgctga aaagtccaac
     1441 cgacatgccg gttaatctgc ggcacagggt attcatcaaa aatttcagtt gcggcctcgc
     1501 cattcgtttg ccggagttct ggtggagcga aatcacaggc gacggcatta aaatgggccg
     1561 ctacgactat ctctgcaacg acgattcgcc gatgctcgaa atcgtatccg tgctcgtgtc
     1621 cgattcgctc actgcgcagg cgtgtggcca ggctgcaata gattttgaca agaaacgggg
     1681 cctgtcgttt gacattctcc agcaggggcc gttaaagata gctggcaaac tcggttatca
     1741 gttcatactc aggcagagtg agacgcaggc tgcggaatcg cagccggccg gcacgaaaaa
     1801 aactgcggag acgaaagatt acgtcataca gatacgccgg ctgctttgcg tgccggacag
     1861 cactgccggc aaaaatcggc attacgcgat gatacttacg ggtttttccg cagatgtcaa
     1921 aaaactctgc gatatcatgg acgaacttac ggattcgttc gacacgctgc cggtagtcaa
     1981 ggaaatgccc aaagctccag ccaaccccgc ctctgacagg gaaggactct gaagcatgac
     2041 gaaagtcggg atgaatgtca ttgagcaggg cgattgtatc aaaatacttg attccgtcga
     2101 agagccgttt gccgatttgg tctttgccga cccgccgttc aatatcggct acaaatacga
     2161 cgtctatgag gacaaactgg cctacgagga atattacgac tggactgaaa aatggatggc
     2221 cgcctgcacg cgggtgatga aacccgacgc gtcattctgg atagccatag gtgctgaata
     2281 tgccgccgaa gtccgtctaa tcggcagaaa actggggctg acgctgcgaa actggattat
     2341 atggcattat acgttcgggc agaacaccaa aaaaaaattc gcccgcagcc atactcacct
     2401 gttttatttt gtgaaagatt caggcagatt tacgttcaac gataaggctg ttcgtgtttt
     2461 ttccgaccgc gaggtttatt atcatgacaa acgtgcaaat ccgcagggcc gtattcctga
     2521 cgacgtatgg gacgaatttc cgcggttatg cgggtcgttt tcggaacggg aaggctggca
     2581 tccgtgtcag atgcccgaat cgctgttggc tcgcataatt cgcacctgtt ccaacgtggg
     2641 cgatgtggtt atcgacccgt ttgccggcag cggcacgacg cttgtggcgg caaaaaaact
     2701 ttcgcgacgc tatttcggca cagagatttc gccgcagtat gtggaaggcg tacggcagag
     2761 attgaataaa actcgcgcgt atgccggcgg ctgtccggaa aaatcgcagc cggctgattg
     2821 ctcggacgat aaaattccgc cggctcattt tgagattctt cgcgatgctt atgccgattt
     2881 tactgtgccg gttacgaaac tgaagacctc cggatttctc atggagcgtt ttgccgaacg
     2941 ttacaatctg aggctcaaat atagtggcgt tgcttgcggg tatacggctc aggcgatatt
     3001 gggttgcctg gaaaaaatga gcaaaaaaat cggtgctctg ccggttataa aatgtcactc
     3061 gattgacgga aagtagcggc cggccatgaa aataagcata gcaacgtgga acgtcaactc
     3121 tgtcaggccg cgaatggaac gcattgctga attccttcgc agagtgccgg ctgacgtgct
     3181 ttgcatccag gaactcaaat gtcgtaccga agatttccca tacgacgcct ttgacgaact
     3241 tggatacaac tgtgccgtca acggccagaa aacgtacaac ggcgtagcag tcgtttctcg
     3301 tctgccgatt gatgatatcc gctgcggcct tggtgatacg gagctggacg agcaggccag
     3361 gctgatatcg gctgagattg caggcttgcg aatttacagc gcatacgtac ctaacggctc
     3421 agacatggag agcgaaaagt atcgtttcaa attgagatgg ctgacgcggc tgagtgaaat
     3481 tctggaagag tcgcactccg ccgatgaacc tgtcgtcatt tgcggcgata cgaatgtgat
     3541 tatcaacgac gatgatgcga atgatatcgt gccgtggcgg actacgggcc ttgcgtgcga
     3601 tgaagtccgc ctcgcgctgc aaaaagtgac ggacttgggg cttgtcgacg tgtttcgcaa
     3661 aataaatccc gaaggcggta aattttcatg gtgggattac aggcagctgg cgtttgtaca
     3721 caacatcggc ttgcggctgg atcatatcct ggctagccgt ccgttggcgg aaaaactctg
     3781 ttcatgtgtt atcgaccgcg aaagcagaaa aggtcaaaaa ccgtccgacc atgccgccgt
     3841 aatcgctgaa ttcaatatct gacctgttgc gtgcgcgaaa aattacaagt ttcaaaaagc
     3901 gagctacagg aaagcgtcaa cttcaaagta ttagagcagt tcaatcttga gagttccttt
     3961 tttgctggtt tgctttgtcc gtggctgcgt cagaaaaact tcagaatact gccaagtatg
     4021 cctcattttc cttccttgcc acgaacaaac ccactggcgc aaaaatatga actctcaatc
     4081 tttcaatgct ctaaaatttc atgattttgg gataggttct gcaaatctca gaacttgatt
     4141 attttgaaat tgcttgcaag ttaccttctt gttttttgaa aatttttact tcattgcatt
     4201 aatcgattcc acaatcgttc tgagattggc ctgcatttcc gctagccgct gcttttcatt
     4261 ttcgacgact tccgccggcg cgcgtgatat gaaattcgca ttgcccagtt tagccgttac
     4321 gcccgcgatg cctttttcca gcgtcgccct gagtttgccc aaccgttcca gttcggctgc
     4381 ggtgtcgatg atgtcgagca cgaaaatttt tcggccgcct atgcttatcg ccgcactgcc
     4441 agccggcgga gttgcaatct gttttgtgac ggtcgcttcg ccgaggcctg cgagtttttc
     4501 aagcagttcg atatttgact gcaatacgtc gttttcgccc gcggattcga tgattatgtt
     4561 tacccgctct ttcggggcga cgttatgctg ctggcgggcg ttgcggattt gtcgtactgc
     4621 gtcggcgagc gttgcgaagg ctgattcgct gtcggcgtcg acaagcgact catccgctgt
     4681 gggccagggg gcggtaatca gcatttccgg tgcaatgccg gccggcccgc gggatgggac
     4741 gacggcattc aggttggccc agattgcttc cgttatgaac ggcataatcg ggtgcatcag
     4801 ccgcagtatg gtgtccagaa catacgcggt taccgctttc ggcgcagctt cgccggcatt
     4861 tatacgcgcc ttggccactt ccagatacca gtcgcagaag tcgtcccaca gaaaatggta
     4921 gagcttttcg gcgaggtcgt tgaagcgata tttttcaatg gcgtcggcgg cgtccatgac
     4981 aacggacgtc atgcgggaaa gtatccaccg gtcagccaga ttggcggatg tgtcagtatc
     5041 gctccactgt ggggcgtcgg atagattcat aatcgcaaag cggctggcgt tgcagagctt
     5101 gttgcagaga ttgcggccga tgtcgaattt cggcgacgag ttcacctcgc ggccgtccgg
     5161 cagggtcatc ttttcaatcg gcatacgcac gtcctgcgtc tgcgtcgtca tgctcacgag
     5221 cgtgtaacgc atggcgtcgg cgccgtggga gtcgattatg tccagaggat ttacgccgtt
     5281 gccgaggctt ttggacattt ttctgccctg cccgtcctgt atcatggcgt gtatgaaaac
     5341 ttcgctgaac ggtatgtcgc cagcgcagta ctggcccatc atgaccatgc gcgagaccca
     5401 cagcgtgata atctcccgcg cggtactcag gacgttgcct ggatagaatt tttctaacag
     5461 ggctttgtcg gctgggtcgt cgctgggcca tttcagagtg ctgaacggcc agagcgcact
     5521 gctgaaccac gtatcgagca cgtcggcgtc gcgctgccag ccttccgatt caatcgcttt
     5581 ttcaagttcg tcaaaaccag gcttgacgca gaggaatgtt accggcccgt tgtcggtctg
     5641 gaagacctgg catgtcgtgc cgtatttaca gcggttgggg tgctcgcctg ctttagacca
     5701 taccggtatc tggtggcccc accataattg gcgggatatc ggccagtcgc ggagattttc
     5761 cagccaggcc tgatagttgt tggcatagcg tgcgggcgtg aatttcagcc ggccgtcgag
     5821 cagcggctga agcgccagac ccgcaagcga gttcagagga acgtcggtgt ccggaatata
     5881 ttttacgccg tcacgctctt cggatttgca gtttgcaatt cgtaatttga aattctctat
     5941 cggttttttc accgcgacgt accactggtc ggagaggtac ggttctatcg gcacgtggct
     6001 gcgatacgaa tggccgacgg agtgcgtgta atcaagcact ttttcaagaa gattttcatt
     6061 tctgaaccac tcgactatag cttcacgggc ctcgaatctg tccatgccga gcagattttt
     6121 ggcttcttcg gtgttgcagt catcccagcc gtggcggtcg gatatgctgc cgtcgggggc
     6181 cattatgttt atcatcgcca gcgagtgtcg ctggcctatc tggtaatcgt cggggtcgtg
     6241 agcgggcgtg actttcagaa aacccgtcga gaaacgggct ttttcgtcag aactttcagg
     6301 gtcgggcaaa actacgtggt cgtcgagtat tatcggtatg agtcggccga ctatcggcag
     6361 gcgaatcatt gtgcctgcga gcactttcgc ccgcgggtca gccggattca tcgccaccgc
     6421 cgtgtcgccg agcatggttt caggccttgt ggtggcgacg gtgacgtgcg tgattttttt
     6481 gccgtttatt tccgccggct tttccagagg atacttcaga taccagaaaa aaccggctat
     6541 gtcttcgtgt tcgacttcgt cgtctgccag caccgtctgg gtggctgggt cccagttcac
     6601 cagccgtttg ccacggtaaa tcaaaccgtc gctgaacagc cgtgaaaatg tttcgcgaac
     6661 ggctttcgcg cagacgctgt ccatcgtgaa tcgcgtgcga gcccagtcgc agctgcagcc
     6721 catgagcttc aactgagaga ggatggtggc ttcgtattca tctttccagg cttggatttt
     6781 ggctacgaaa tcttcgcgtt tgaagtcggt gcgacgtttg ccttcttcgg cgaggacgcg
     6841 tttttcgacg acggtctgtg tggctatgcc ggcgtggtct gtgcccggca tccagagcgt
     6901 tgcaaacccg ctcatgcgtt tgaagcggat gatgacgtcc tgcaacgtat tgttaagtgc
     6961 atggcccagg tgcagggctg cggtgacgtt cggcgggggg atgaccatgc agaaactttc
     7021 acgtggatcg ttggctgccg ccgcaaagga gttgtgtttc tgccacttct cgtacgcttc
     7081 attttcgacg gctcgcgggt cgtagacctt tgccagttca ttgctcattt ccataccttc
     7141 taaacgtaaa tcccataata aacgacaaaa tcgaattaaa gaagttacag gaaacaggca
     7201 acaaattcca agtaattttt aactttcagg taaaaccggc acggtttgtc gtatcggctt
     7261 gtatcttgtt tcttttaaaa attgcctgca agttgtacct tgtaactttt ctgtgattat
     7321 gcgacgcaat cgcagttgga ggcggaattt gtcggagcag ttcccgaagg aaaagttttt
     7381 tgggaattgc tctacttcgc cgcttccctg tcgttgagca gctgaaattc tatggcgtcg
     7441 accattgccc gccatgatgc gtcgatgata ttttcgccta cgccgaccga gccgaagtat
     7501 ttgtctccgc ttcgccagtc tatgactacc cggacttttg ccgccgtttc agctgcggtg
     7561 ttgacgacgc agacgcggta gtccatcaga tgcagcctgt ccaggccgtg atatttttcc
     7621 cgcagtgcgt ccttcaacgc gccagcgagt gaatcgacag ggccgtcgcc ttcgcggacg
     7681 gtatgcatag tctggccgtc gtcgaacgcc aacttcaccg tcgcttccgt cgtagccttt
     7741 tcactgtcgc gtttgagtat gatacagcga taatggtcaa gtttccacaa gggggtatac
     7801 catttgcccc ctagcgtttc gcggattacc atctcgaaac tcgcctcggc acgttcgaat
     7861 tcgtagcctt cgttctccag ctccactacg cgggccagaa tttttttctg tgcgtcgcgg
     7921 tctttatcta atttgaattt cgccggaacg gtggcagcta tgttgctgac gcctgcaagt
     7981 tcgctgacga gtattctgcg cgtattgccg acggtttccg gcgtaacgtg ttcgtagctg
     8041 cacgatatct tctgtacggc gtgcacgtgc atgccgccct tgtgtgcgaa ggcgttgctg
     8101 ccgacgtatg gctgattgtc gcggaagttc atgttggaaa gttcataaaa accgcggctg
     8161 acttcggtca gtttggctat ggcgccttcc gtgagtactt tccggccata tttgagagcg
     8221 agattcgccg atacgctcac caggtctgca ttgccgcagc gttcgcctac gccgtttatc
     8281 gtgccttgca cctgtattat gccggcgttg actgccgcca gactgtttgc gacggccatg
     8341 tcgcagtcat tgtggcagtg tatgccgagc ttggcggctg gcagggcgtt cagaacggtt
     8401 ccgactacgc tggagatgta ttccggcatt gtgccgccgt tggtgtcgca gagtaccagc
     8461 cgctgtgcgc cggcatccat agctgcggcg agggttttta gggcatattc catgttggcc
     8521 cgatagccgt caaaaaaatg ttccgcatca aatattacga atcggccggc ggcggctagg
     8581 aacgcgacgg attcggctat catgtcgatg ttttcctgct tcgatacgcc cagcacttct
     8641 tttacgtgca tgtcccaagt tttgccgacg atggtcacca caggcgcttc gctgtgcagc
     8701 agggcattca tgccggcgtc gtcggcggac ttggtggctt tacggcgggt catgccgaac
     8761 gccgccagac gcgcgtttgc cagacggatt ttccgcattt cctggaaaaa ctgctcatct
     8821 ttgggattgc tcagcggata accgccctcg atgtaatcca cgccgagttc gtcgaacatt
     8881 ctggcggctg tgagtttgtc ctgaagcgaa aggctgacgc ccttcgcctg tgtgccgtcg
     8941 cgtaatgtgg tgtcgtaaag ttccactttt tcgccggttt tcatttgtgc atccttcaaa
     9001 aattatcctg aacgctttta aatatcgttt actgcaatac ggacgacttt gacaatcata
     9061 ttgcggcttt gcttttatct gtctgtcgct gaatctttcg gtctcgtaac ggaagcggat
     9121 gtcaaatcag cttctaaaaa aaaggccctg agggatatta tcgggtctct cagggcaggt
     9181 atgaaagaat gtagtccgct acaccctaag gtccggttga aacatggttg atggcaataa
     9241 taatattgcc tgagcagaca ctcggcgccc tctttataac aattattatt gtatttttgc
     9301 cttgcgcctg catcaagtca tgttcctttc tgatatttga ccttagtgca tgattcaatt
     9361 atattaacag agacgccggc gtcaatgatt ttgttgcggt tttgccgcgt tttggaaaat
     9421 ttgtttacgt cgtgacggat gatttttatg caaaatatca ttctttttcg ggcggtttga
     9481 gggaatgttt ttataatccc tgtggaattt ccaaaaaaac ctttatgttc atgcctttgg
     9541 aacatatact ggaacatgtc cgatttccgg acattcaata ttttcgcgat tcgagggaat
     9601 caagcgtgga aagaggtgat aatgaatttg gttgattttc agggaaattc cattgttttt
     9661 ataggaaatt atttgccgag gcagtgcgga atagccactt ttacccacga cctctgcgaa
     9721 gccgtcgccg ctgaagttgc cgacaaaacg ccggtttttg ctgtggcgat gaatgacagg
     9781 cccgacggct atgcctatcc gcatcgtgtg cattttgaaa ttcgccagaa ttctcagaat
     9841 gattaccggc ttgccgccga atatctgaat atgcagcagc cagccgccat atgtcttcag
     9901 catgaatacg gcatatttgg cggcccatac gggtcgcaca tcctgagttt gttgcgtcgt
     9961 ctgcggcggc cgctgattac cacgctccac acggtgctgg aatcgccttc cgaacagcag
    10021 aagctcattt tacaggaaat cacccgcctt tccacacgcg tggtggttat ggcggaaatg
    10081 ggtaaaaaat ttcttgaaga cgttttccat gtgcctgctg aaaaaatcgc cgtaattcct
    10141 cacggaatcc ccgacgtgcc gtttgttgac ccacattttt tcaaagatca gttcggcgta
    10201 gaagggaaaa aggtcatgtt gacctttggc cttctttcgc ctggcaaggg ccttgaatac
    10261 gccatagaag ccatgcccgc aatcgtcaaa aaacatccgg aactcgtcta tataatcctc
    10321 ggcgccactc atccgcacgt caaagccgag tacggtgagg aataccgtaa cggcctgacc
    10381 cgcagggtca acgaacttgg cgtacgtgac aatgtgatat tcgtcaatcg tttcgtggtg
    10441 ctcaaggaac tctgtgaata tcttggcgct gccgagttat atgtaacgcc gtatctcaac
    10501 gaggcgcaga tgacctccgg cacgctggca tatgcgatgg gaaccggcaa ggcgatagtg
    10561 tctacgccgt actggcacgc cagagaggtc ttggccgacg gccgcggtac actggtgccg
    10621 ttccgcaaca gccaggctgt tgccgatgct gtcaataacc tgcttgacaa tgaaaacgaa
    10681 cttaatgcca tgcgaaagaa cggctataat tactgccggc ggatgatatg gccgtgcgtc
    10741 gcagagtcgt atctgaatct tttcactcag gctggcgaag cgtggcagga agaccagcgt
    10801 accgccaagc gacatggcga tataaaacct cgccgccgcg aaaaattcga tttgcctgaa
    10861 atcgacctca aacacatgca gttgatgacg gacgacacgg gcatcatcca gcattgcgtc
    10921 tatataacgc ctgaccgtta tgagggttat tgcgtggatg ataatgccag ggcgctgata
    10981 gtcgcaggga tgaactggca gctgctcaag gatgaatccg taattcccat gatgaacacg
    11041 tacctgtcgt ttttgtatta ttctcaggat aagaagagcg gccgattcag gaatttcatg
    11101 aattacgacc gtacgtggcg agatgaaaaa cccagtgaag atgcccacgg ccgggctatg
    11161 tgggccctcg gcgaagccgt tgccaactgt cctcacgaaa gcatgatagc tcttgcgtcc
    11221 cgtatgttcc aggttggcct cggttctgta aagtcattta cgtctccgcg tgcatgggca
    11281 tttacgctgt gcggcataca tgcgtatttg cggcggttca gcggcgattc acgaatacgt
    11341 aggcatcgcg ccgcactcag tcagaagctt ttcgacctgt tccggctgaa cgtgacggag
    11401 gactggcggt ggtgcgagga catcgtcgcc tacgcctcag ccaaattgcc gcacgtgctg
    11461 atgatgagcg gcagatggat gcagcgcggc gacatggtgg acctcggccg ccgggcgctt
    11521 ggctggctga tagacataca gacaaatgat gacggatttt tttcattcat cggttcggac
    11581 ggctggtaca ggcggggcca taaaaaagcc cattatgccc agcagcccat agaggctcac
    11641 gccatgctgg atgcgtgtat cgaggcgttt catattacac atgagaatta ctggatagac
    11701 caggctcaga aggcgtttta ctggtttttg ggcgaaaatg acctgcggcg gaacttgtat
    11761 gatttcacta ccggcggctg tcgtgacggt ttgcaggctg acggcgttaa tgaaaatcag
    11821 ggtgctgaaa gtacgcttgc gtggctgatg agcctccagc agatgcatgc gctccaggaa
    11881 gaactttccc tggctgaaat cgctgccgag cagaacgatg attcgccgaa cggcttagcc
    11941 gtcaaaacag aataatcaat tattgacgat gtttttttag cgccgcagca atgcggattt
    12001 cgtttttttc gattgtttta ttgttttaga tttgatgcag gagttgttgt catgtctgac
    12061 ccgatagtga tgattagcag ttatccgccg cggctttgcg gcatagcgac cttttgcgaa
    12121 gaagcccgcg agttcatcaa aaaagccaat cccgacaggg atgtacttgt catttctcat
    12181 actgacggca aaggagacgg cgtttttccg attattgact tgtccagccg tctctggtgg
    12241 aaaccggtcg ccgaaaaaat ccaccaactc aagccgcatg tcgttcacat cgagcatgaa
    12301 tacggcttgt ataatttttt ggatgcccgc ggcatatccg acggcaatcg tgggctgttg
    12361 aatctgctgg aagcaatcag cgacgttcca atagtgattg aaccccatac cgttcatggc
    12421 cgtctgaccg actttgaggc ggaattcatt taccagatgg ctgcgcgagt ggatgtttgt
    12481 cttttcaagt gtcattatca gaaatggcgg ctggactgga gttttagcgg cttgggttgg
    12541 cctgtgccgg aaaacatcat ggtcgtgccg cacggcgccc gtgcggacag gcgatatgcc
    12601 gtttccgaag tgccggaact tcgcaaggaa ctcaatctcg acagaaccaa actttccaat
    12661 catgtagtgg ggctcatcgg ctggatacag tccaacaagc ggtgggatat tttgatggaa
    12721 atgtgggagg aagtggcggc ggaaataaag cagcgaacga atcaggattg ggatttgctg
    12781 gcagccggca tgttacgtga cccacatcat aaggctgatt atgaaaaata tcgctcggaa
    12841 gtggaaatac tcgagcgcaa ggggctggcc cgctattacg agtttgtgcc gcgaggcgag
    12901 atttattaca aaatgatggc tatttgcgac ttcatagttc tgccgagtac cgacgaaact
    12961 cagtccggca cacttgcgag aattatcgcc ctgaataagc cctacatcac caccgccccg
    13021 atggaaggcc ttaccgccca gacgcttgaa tccggcggcg gcctgctttt cacgaatcgc
    13081 gaaatgctca aacgtcaggt catcgcactg gcgtgcagcc gcaaacgaag aatgcagctc
    13141 ggcgataacc tcagaaatta tctcgacaat gtcgtgtcat gggaaatcgt tgccggccaa
    13201 tataacgaag catatgaact cgccaggaat gcaaaaaaaa ccggcgtcaa agtcaaactg
    13261 ccgaaagaat tttgaaaaac cgccaatgtt ctgcagctgc ttttaaaaaa aaataaaata
    13321 aataggaata atgggttgac tcggttggcg gattgtgtat tattacccgc ccaagacaag
    13381 gatgtccgaa agggacattg ccagttatta taaaacagct tagcgacttc agacgacgcc
    13441 tgtttaagcc tgaaattaaa gcatcgaaat aagattgcgt aatttggttt tcatggcgaa
    13501 gtcggcaagg tctactgata cagacgggct tgtagctcag ttggttagag cgcgcccctg
    13561 ataagggcga ggtcgctggt tcgactccag ccaggcccat aaaaatgggc ttggcgtatc
    13621 tctcgattaa ggccgttgcc cagccgggga cgtagctcaa ttgggagagc actagctttg
    13681 caagctaggg gttgtcggtt cgatcccgat cgtctccatg ttgattttgg cggctgataa
    13741 gcggttgttt tagcagaaaa gaataagtga ataaaaaaat tcaaaatttt tgaattttaa
    13801 acttgctatc gaaaatgccg attgataata tcttcggtct gcaacttgaa aagcgtattg
    13861 agctgtgagt tgcggcgagc cgtaagggct tgttcgataa agagttgtaa aaaacgaaaa
    13921 aatatttgac ttgcaatttg atgtgcaagt tgataatata acgaatctcg gccttatggg
    13981 gtaaaacctg taacgtcgta ggccttcgag ccgcaagggc tttttaaggc tgaaaaggat
    14041 ttttgacaaa ttcataagag tgaagatttt tctgtcaagg ttcaccgagt gtaaactttt
    14101 ggcgacttaa ttaaaaaggt cacgggctgt taaggcttgt gacatatgag gttaagctag
    14161 taagagtgta tggtggatgt ctaggcgtaa tgaggcgatg aaggacgttg taggctgcga
    14221 tatgccgcgg ggagctgtca aacaagcatt gatccgcgga tttccgaatg aggcaactcg
    14281 ttacgactgt gcaagcagat cgtatcacca gcatctgaat gtataggatg cttggggcta
    14341 acgtagagaa ctgaaacatc ttagtatcta caggaataga aatcaaccga gactccgtaa
    14401 gtagcggcga gcgaaagcgg actagcccaa accgggagca tgcttccggt gttgcgggcg
    14461 gctgacatgg agttggagca ttaatggaag tgtctggaaa gtcacacgaa acagggtgaa
    14521 agtcccgtac atgacaatgt gaagacccta agccgtacca gagtagcgtg gatgccgtga
    14581 aactccgcgt gaagctgggg ggcccaccct ccaaggctaa gtactccatt acgaccgata
    14641 gcgatgagta acgcgagtga aagatgaaaa gtaccccttt taggggagtt aaaagtacct
    14701 gaaaccatat acttacagtt agtcgcagcc ctatgcccag caatgggaat gggttacggc
    14761 gtgccttttg cataatgaac cggcgagtta ctgtatacgg cttggctaag cgtctcaggc
    14821 gtggagccga agcgaaagcg agtcttaata gggcgtatgt cgtgtgcagt agacccgaaa
    14881 ctgagtgatc tactcatggt caggttgaag gaacggtaag acgttctgga ggaccgaacc
    14941 cgttaacgtt gaaaagttat gggatgaact gtgaggagga gtgaaagtct aatcaaactc
    15001 agagatatct ggttctctcc gaaacagctt ttgggctgac ctcgagtcac tacttgatgg
    15061 gggtagagca ctggtaagat cgagggccct tcccgggtac ctcctcttaa taaactccga
    15121 ataccatcaa gactatctcg ggagttagac tgcgggtgat aacattcgtg gtcgaaaggg
    15181 aaacaaccca gatcgccgtc taaggtcccc aatgcatgct aagtgcttaa ggaagttgag
    15241 ttgcacaaac agccaggatg ttggcttaga agcagccacc atttaaacag tgcgtaatag
    15301 ctgactggtc gagcaattca gcgccgataa tgatcgggac taagcatgtt accgaagacg
    15361 cgggctcaca tagtgagcgg taggagagcg tagctgtcga cgttgaagac gtaccgtaag
    15421 gagcgttgga gtggcagcta gtgagaatgc cggtatgagt aacgataaag caggtgaaaa
    15481 tcctgctcgc cgaaaaccta aggtttcctg gggaaggtaa atccgcccag ggttagtcgg
    15541 gccctaaggc aaggccgaaa ggcgtagtcg atggatagca ggttaatatt cctgcactgc
    15601 gcgtgtaggt tgaagcacag atgacgcatt tacgaaactg atcctcccgt tagtcgagga
    15661 ggttgctgat ggggtaactc attggctcga gcccgctttg ttggcgaagt cagcgtagta
    15721 ggtgcctgga aaagctctgt gtggacgaat cgcgtatccg tactaaaact gacacaggta
    15781 ggtgaggtga gtaacctcag gcgctcgaga gaaccgttgt taaggaacta ggcaaattcc
    15841 ccccgtacct tcggaataag gggtccctcc cggattctgc atttatgctg atgatggagg
    15901 gcgcagagaa atggcactag cgactgttta tcaaaaacac aggtctctgc taactggcaa
    15961 caggatgtat agagactgac gcttgcccgg tgccggaagg tcaaggaagt gggttagctt
    16021 cggcgaagct cgcgacttaa gccccggtaa acggcggccg taactatgac ggtcctaagg
    16081 tagcgaagtt ccttgtcggg taagttccga cgtgcatgaa tggcgtaacg actggtgcac
    16141 tgtctcaaca acggactcgg tgaaattaca aacgcggtga agatgccgcg ttcccgccgt
    16201 aagacggaaa gaccccgtga acctttactg tagcctggta ttgatgcctt gtaatgcatg
    16261 cgtaggatag gtgggagatg ttgatctgcc ggttttggcc ggtagggagt catccttgaa
    16321 ataccaccct tgtgctatga gtcacctaac cgtgtccctt gaatcagggc atgggacagt
    16381 gcttggtggg cagtttaact ggggcggttt cctccaaaat cgtaacggag gagtccaaag
    16441 gtaccctcag tgcggttggc aatcgcacga agagtgtaag ggcaaaaggg tgcttgactg
    16501 cgagacctat aagtcgagca ggaacgaaag ttgggcttag tgacccggtg gtcccgtgtg
    16561 gaagggccat cgcttaacag ataaaaggta ctccggggat aacaggctga tcgctcccga
    16621 gcgtccatag cggcggagcg gtttggcacc tcgatgtcgg ctcatcacat cctggggctg
    16681 aaggcggtcc caagggttcg gctgttcgcc gattaaagtg gtacgcgagc tgggttca
//
LOCUS       DAONFI010000006        16642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_3674_length_16642_cov_4.309821, whole genome shotgun sequence.
ACCESSION   DAONFI010000006 DAONFI010000000
VERSION     DAONFI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3674_length_16642_cov_4.309821"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..827
                     /locus_tag="PLK08_00440"
     CDS             273..827
                     /locus_tag="PLK08_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS51997.1"
                     /translation="MSEENDALHDTAALRSLAMSINNASDDEAANEVFTPTDGQPAES
                     TDDFFEAAAEGKPAEVEEVLTVASEEREELVRVEKSIGSKNGVALARIQTLQRTIQMR
                     KIGIPILSAIAGILFLISVATLYQAYNALPEQIDANPLLQNAGLFAAISIFLGVSLLA
                     GVGFFAFEISRYNASIKKIEAAML"
     gene            987..2213
                     /locus_tag="PLK08_00445"
     CDS             987..2213
                     /locus_tag="PLK08_00445"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPS51998.1"
                     /translation="MFHPIEEILQDLREGKMVVLVDDESRENEGDLLCAAEKVTPEII
                     NFMVKFGRGLICLPMTRQRADELDLPPQTKFNTSAFSTAFTVTIDAVKGVTTGVSAAD
                     RAKTILLATRDDAKAADFARPGHIFPLRAVEGGVLLRVGQTEGGVDLTRLAGLKPMAV
                     ICEVMNEDGTMARRDDLDVFCKKHNLKMCTVADIVRYRLEHEQLIKREVEIDLPTKYG
                     NFRVIAYSSLVDPQPHLALCCGGVGEKDSDGRTIIHEEPVLVRVHSECLTGDVLGSLR
                     CDCQQQLHTALETIAKAGKGAVVYMRQEGRGIGLINKLRAYKLQIEDGLDTVQANEHL
                     GFQPDQRDYGIGNQILRDLGLRKLRLMTNNPEKIYGLSGYGLEVAERVAIKIKPCQYN
                     AKYLRTKRDKMGHMLD"
     gene            2291..3277
                     /locus_tag="PLK08_00450"
     CDS             2291..3277
                     /locus_tag="PLK08_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS51999.1"
                     /translation="MAGRPIWCAWQVTYRCNFRCKFCGYWNDPLGRQPEPSVDEYKIG
                     AQKLATFGSLLVSLAGGEPLLRKDLPQIVSAISEYHLPFITTNGSLVTQENAAALMAA
                     GLWGVSVSIDYADPKKHDDARNRQGAWQRAWQAVEYFSKARTQEFQRVNVMAVLLDNN
                     IDELETIMKMAAERNAYFMVQPYGYLKTGSHDYECRHSNISSRLLDMWRRNRNFLSNP
                     TYLKKFDAFHNGGIEGCKAGRAFFNIDSTGDIAICVENKHAPIANLYTDSQYKILNAL
                     KSASRRNTCKCCWYNCRGEVESLYNFKSLIQSLPTFFFNHGVADGGKMGRWT"
     gene            complement(3308..4117)
                     /locus_tag="PLK08_00455"
     CDS             complement(3308..4117)
                     /locus_tag="PLK08_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPS52000.1"
                     /translation="MNQTSEIYRDEKSLLHVRRICAEILDMFVPNVCASCGAKPVGHS
                     MLCEECRIKLLNCVTLPYCPFCGTSLGPHLTSKANSGCYDCPVPMPRFRRVYRIGPYA
                     GVLKPIIRQIKYHSQWAMIEPLTRLLAEHIPNVSQTQFDLVAPVPMFWARRILRNGNH
                     SWMMAKCLAGKLNLPASREIIRIRNTPQQAHFCRSRRMENVKDAFKIRNPQLMSGLKI
                     LLVDDVVTTGATADEAVRTLLDAGASEVHLAVFAKAGTPVPYEDRHPESKS"
     gene            complement(4114..6159)
                     /locus_tag="PLK08_00460"
     CDS             complement(4114..6159)
                     /locus_tag="PLK08_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52001.1"
                     /translation="MQKIPLKKFFRNHRILRWELRLLILLIIACIIGIFALPAWRDYA
                     AIQKLGAGDYQQQRDAAIAAATRASNRPAMIKRLIASLDTPDKAKFIAIHDILFHFRK
                     FTSKHVQPVWLDRYNLIELEKLLAADKSPQTQPAENRKAAAMSRRTFIYDFIINGDDN
                     RYVRRALELVHVDPLPAVRQAAAALAAELGNDDILKKLLADSDPQVAAEATIAVSVAN
                     RTACLNDVIAIFNRSIRASRVEPDLIAACAMALANMQATTPDLKKIASLAIETDDEIL
                     REKLSFILPLIADQDITDALAEYFKKCSAAQKCPNAMMLSAGAKMNMPIAAAIARDIL
                     IRAIDPKKAAGMMQSHIIAAMQTLDQMGIPCRREIYDFIKTYWQPGRHLMLIPAARLL
                     GEQAVTPNQPAGAPTQEQCLELLRQAATWIRQDRQGNFTNTPKSSAAAAVAAWLISPA
                     HIEFLISSGDNPDMETMKVEYTTAFLVREAVTGEPAFAGDYIAWNIGATHSTEAYALG
                     QWLLPGENERIELNPDVRAAGALLLGFATDSANERASAEARIDRRLNREDFFAKGSCQ
                     CALLTLWPGREVLHEQVRNLLWIPDFPRRRVWTALMFAGDRIVLDSLLLNAATNLNDV
                     AESFVVTEMNPVINTLAPSLTTPSAAADESTRLLQMRFMRMQWACGRNSIAYGMPQQ"
     gene            6231..7616
                     /locus_tag="PLK08_00465"
     CDS             6231..7616
                     /locus_tag="PLK08_00465"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPS52002.1"
                     /translation="MNETRIERDSMGEMPVPAEALYGPQTARAVENFPVSGWAMPREF
                     IAAMGWIKQAAAATHCEAGRLDGELADAIIQAAGEVISGKLDAHFPVDVFQTGSGTSS
                     NMNVNEVIARRASQILGGRKVHPNDDVNRSQSSNDVVPTAMHVSAAVAMHDSLLPALR
                     VLKEMLDIKAGEFDGIVKIGRTHLMDAVPVRLGQEFSGYASQIEAAIVVTQQAMGSLC
                     ELPIGGTAVGTGLNAPADFAQSVCLYLGEELHLPFVETSNHFAASGSRDAALQASSAM
                     RQVALAMGKIASDIRLLGSGPRCGIGELELPAVQPGSSIMPGKVNPVICESVIQVACQ
                     VVGCDAAIAAGVTGGVGGILDLNLAMPMITMNLITQIKLLANVARVFGEKCLAGLKAN
                     VSRCGELVEKSLAMVTVLAPIVGYDKAAELAHEAMKSGKTIRELCMEKKILPADKLTE
                     LLNPAAQTGQQ"
     gene            complement(7800..9209)
                     /locus_tag="PLK08_00470"
     CDS             complement(7800..9209)
                     /locus_tag="PLK08_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPS52003.1"
                     /translation="MACVAAVMSGCKVGPNFTKPETQMPAGWIEKAQQTPQTQPAVEK
                     VNLSEWWKIFNDAELTSLINEAMAANLDVCIAKTRIRQARGNYGIAVSGIGPTLDGGS
                     SYQRGQSGNTPVSNQYQAGFDSGWEIDIFGGVQRNIESAEAQLQASVESLRDVQVTLA
                     AEVARYYIELRTLQQRIIITKQNLQAQQRSEAVTRKKFEGGFVSGLDVANSRAQVAAT
                     SAQIPLLESSERQAIYALSVLMNREPSALLERLSKSATIPIAPPAVPVGIPSDLLRRR
                     PDIRMAEANVHSATANIGVAVADLFPKFNFGSSLGWSSADLNTMFNPASRLWSFGPSM
                     SWNIFQSGATLANIDVQKALKDESLLTYRQTVLNAVQEVETALIQSQMEQEHVKALKE
                     AVVQNQKAVEISTVLYSEGNTDFLNVLSAQQSLLNTQSSLIQSSGNVSLYLISLYKAL
                     GGGWDEDIPLKEMEYSNSF"
     gene            complement(9337..10707)
                     /locus_tag="PLK08_00475"
     CDS             complement(9337..10707)
                     /locus_tag="PLK08_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPS52004.1"
                     /translation="MKIYRTLTTATKTLRRNPLRAMLTTLGIVIGIGSVIAMMEIGEG
                     SSSAVRESIVNMGSNMLIISPGSISSSGVNQGSGSVMSLTPDDCEALKKECPSLRAVT
                     PIVCWSTAPVVYGNKNWTPRRMWGVTAAYFEIMVWGGNFNTSRTFTERDVQTSARICV
                     IGQTVVHELFNGKNPLGKEVRINNVPLIVVGVLDKKGANMFGGDLDDIVVAPWTTVKY
                     RVNGTSSGSSSQSSSSESTSTDLSNPVPCKLPSLYPSQSSTQTKNNPMLTRFKSIDYI
                     MASVIDSNHIQQATDEITAVLRRRHDIQPKDKNDFEVFNRTEMLDMLTSSTKLMTNLL
                     LCVATISLIVGGVGIMNIMLVSVTERTREIGLRMAVGARSRDILQQFLTESVILCLVG
                     GTIGILLGHGGSLLLEFFLKWPVETSSFAIISAVSVAVGIGIIFGFYPAWKAAKLDPI
                     EALRYE"
     gene            complement(10704..11450)
                     /locus_tag="PLK08_00480"
     CDS             complement(10704..11450)
                     /locus_tag="PLK08_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS52005.1"
                     /translation="MEFLELVEIYKTYRIGNMEVPVLKGVSLKISRGEFVALMGASGS
                     GKTTLMNILGCLDRPTAGEYWLDGHEISKLSPDARALIRNQKLGFVFQSFNLLARTSA
                     VDNVAMPLAYTRHHISEHECRKRASEILCKVGLEHRMHHEPSQLSGGQQQRVAIARAL
                     INHPPILFADEPTGNLDSHTSQEVLQMFRKLNVEDGLTIILVTHDADVAHSADRVIHI
                     RDGMIVDDCPKSEKPSVCHEDKVTSSGGAK"
     gene            complement(11483..12832)
                     /locus_tag="PLK08_00485"
     CDS             complement(11483..12832)
                     /locus_tag="PLK08_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPS52006.1"
                     /translation="MKHIVRKSIFAIVILAIIAGIVGWIMQGEAEEKMTFDTVDVTRD
                     DISISIAASGTLEPEEVVDVGAQISGRVLSFGKDKNGKTIDYGSIVEKDSVIAKIDDV
                     LYLADEAEAQANVSSAKAGVQLEKANLGQLQAKFLQAKRDWERAQRLGPSDALAKASY
                     DAYQATYDAAKANVDVGKATILQAEATLTLKEKSLWRAQRNLEYCTIKSPITGMIIDR
                     RVSIGQTVTASMNTPSLFLIARDLRRMQVWVLVNEADITKIYKDQPVTFTVDALPDET
                     FEGKVWKIRPNAAMTQNIVTFTVEIVTDNFKGKLKPYLTANVRFIVSKRENVLTVPLA
                     ALSWNPKKEQIAQEYRDYKVAESQPAAEAKTTATQPSSQPADKTIKTTQDILWVKDGE
                     YVKPVEVSVGLTNEAYAEVSGKDLQEHMQVVIGMKPVEEKSDDYVNPFLPFSSKRKR"
     gene            13275..14054
                     /locus_tag="PLK08_00490"
     CDS             13275..14054
                     /locus_tag="PLK08_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52007.1"
                     /translation="MLSFITINPPGSFSTVPIRQWVAGINLEGRCHEYHPDGDFSQEC
                     YEFGVIKPNFPHHWEVPADGIPWKSLHFFFTPSVSMLPLLQFDEIRPGFMKLPLAESP
                     FLYKVQRMLFKSHYLLNSHWLNCVDLAMNALEGALLWCRAEVERVQQKTDSRILTAIE
                     YMNRHIAEKIYLEDIAKASFLSVPRLMSLFKANIGLSPMAYLDDERMERASKMLTTGY
                     FSIKEIAAMTGYPDPAYFSKRFLQHFRMSPLAYRKKHITDV"
     gene            14253..16001
                     /locus_tag="PLK08_00495"
     CDS             14253..16001
                     /locus_tag="PLK08_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPS52008.1"
                     /translation="MIIITLLAGGCIIGCVDPGKYDVNDVYKLQQSIMDRSPQNRSDS
                     GTGPMIPSENSVPRIKMEKNPKTNRYEVSLSLRETVMRALANNPQIAVVSFTPQISRE
                     LVVQAASQFDYTIFGGYEFTHTESSNTTTTGNITGIPTGKLNANDFQFGVQQQTITGA
                     QWSLAASMISTRYRPTTNDWDYDNSIAFNVTQPLLRGAGTEVNLANLRIARLNYKISM
                     SQFNQNVQQTVVSVMSAYYNLIKARQLVVIAENLLAVTEQTYRKVSERHSLDATKVQI
                     KQSETAVRERQAALVLARKNARDAQDALLVLLGDEQLNLLGRNMDIIPATTLTSLPVL
                     IDPTDQYLTALRLNPQLEQLRLAVSQSDINIMVAENQALPSLNLTAGATLAGDSSGTN
                     RGAWNGMLDARYTSYNAALMFEYPLGNRQRLSAVRQSKLQRRQAISNLQSQSDQLAQQ
                     IRESIRQINTQYEEIKIRRQALDAAKEQLDALDVLEKIRAKLTPEFLNLKLNAQATVA
                     TSESNLIDAQVLYNVAMLNLSRYTGTVLELNQVQLSLPVVTQPDNSANPLTLTPAPPN
                     DAPVPLPSMLQSSSSK"
     gene            complement(16063..>16642)
                     /locus_tag="PLK08_00500"
     CDS             complement(16063..>16642)
                     /locus_tag="PLK08_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52009.1"
                     /translation="DVKSSWSAAADLSDKLHILYTDRAYDSILSRAPLMYDELWTGGK
                     CMYKMECVVADGGELIIYAPHIHEIAVTHGRIIEEVGYHSLPFFINQWERYKDYPWGV
                     LAHSTHVRGIGKWINGREVPRVTVTLATGIPEETCRRIGMNYRNPASIDISQWQGHES
                     EGKLYVPKAGEMLYRLKNAPEWQQCNRAYSGS"
BASE COUNT         3929 a         4077 c         4310 g         4326 t
ORIGIN      
        1 gcgaaagcct gatgaatatg atgctacggg ttttaacccg tagagattca cttttattat
       61 gccggttgat atgcggcata catatttacc gcttgagcct ctgatatttt aaaaaaatta
      121 cgacgaattt cggctaaccg agttgctata ataacggttt gcagttggta atatggctta
      181 attgtgttat aattccgtat tcgccaaaaa atgactcgct tgtgcgttta cggcgagaca
      241 tcagtaaatg gctgtaagca ggagaataag atttgagcga agagaatgac gcccttcacg
      301 atactgccgc gctgcggtcg ttggcaatga gcattaataa tgcgtctgac gatgaggcag
      361 ctaatgaggt ttttacgccg acggatggcc agcctgccga atccactgac gatttttttg
      421 aagccgccgc cgaaggcaaa cccgccgagg ttgaagaagt ccttacggtc gcttccgaag
      481 agcgggaaga actcgttcgc gtcgagaaaa gtatcggcag taaaaatggc gttgccctgg
      541 cacgcataca gactttgcag cggactatcc agatgagaaa gataggcata ccgattcttt
      601 ccgcaattgc cggaatattg tttttgatat cggttgccac cctgtatcag gcctacaatg
      661 ccctgccgga acagatagat gcaaatccgc tgctgcagaa tgctggactt tttgcggcaa
      721 taagcatatt tttaggcgtt tccctgctag ccggcgtcgg cttcttcgcg tttgaaatat
      781 cgcggtataa cgcctcgata aaaaagattg aagcggccat gctttgattt tgaaattacc
      841 ctgtaaaaac atggcctgaa gtgtcgtttt aagcgataag aaaatctcat gattttcgat
      901 aatcatcgcg cttcatgtat tttaccatgt taagcgtgac actggaaaac atgttttata
      961 caacgcaaaa cggaatcgga aaaaaaatgt ttcatccaat tgaagaaatt cttcaggacc
     1021 ttcgtgaagg aaaaatggtc gtccttgttg acgatgagtc gcgggaaaat gagggcgacc
     1081 ttctctgtgc agccgaaaaa gttacgcctg agataatcaa tttcatggtc aagttcggcc
     1141 gtggcctgat atgtctgccg atgactcgcc agcgggccga cgaacttgat ttgccgccgc
     1201 agaccaaatt caacacgtca gcgttttcca ccgcgtttac cgttaccatt gatgccgtca
     1261 aaggtgtgac gaccggcgta agcgcggccg accgcgcgaa aaccattctg ctcgctacgc
     1321 gggatgacgc caaagccgct gattttgccc ggccgggcca tattttcccg cttcgcgcgg
     1381 ttgaaggcgg cgtgttgctt cgggtcggcc agacagaagg cggcgttgac ctgaccaggc
     1441 tggctggcct aaagccgatg gctgtcatct gcgaagtcat gaatgaagac ggtacgatgg
     1501 ctcgccgcga cgaccttgac gtcttttgta aaaagcataa tctcaaaatg tgcaccgtgg
     1561 cggacatagt ccgctatcgt ctcgaacacg agcagctgat aaaacgggaa gttgagattg
     1621 acctgccgac gaagtacggc aattttcgcg tcatagcgta ttcatcgctg gtagatcctc
     1681 agccgcatct ggcgctctgt tgcggcggcg tcggcgaaaa agattctgac ggccggacga
     1741 taattcatga agagccggta cttgtgcggg ttcacagcga atgtcttacc ggcgatgtgc
     1801 ttggttcgct tcgctgcgac tgtcagcagc agcttcacac ggcactggag accatcgcca
     1861 aagccggcaa aggcgccgtc gtgtacatga gacaggaagg ccgtggcatc ggcctgatta
     1921 ataaattgcg tgcttataag ctccagattg aagatggact ggataccgtg caggctaatg
     1981 agcatcttgg ttttcagccg gaccagcgcg attacggaat cggcaatcag atactgcggg
     2041 acctgggcct gcggaagctc aggcttatga ccaataatcc cgaaaaaatt tacggcctta
     2101 gcggttacgg ccttgaggtg gctgagcggg ttgccataaa aatcaaaccc tgccagtata
     2161 acgccaaata tctccgcacg aaaagagaca agatggggca tatgctcgat tgacgttttg
     2221 aatcgggtcc ggctgggtga catagatgtt ttcctcatgg cgaaaaaaaa caagactgtt
     2281 tcgttcctgg gtggcagggc gacccatatg gtgtgcctgg caggtcacgt atcgttgcaa
     2341 tttccgttgc aagttttgcg gatactggaa cgacccgctg ggccgtcagc ctgagccgtc
     2401 ggtggatgaa tataaaatcg gtgcgcaaaa acttgccaca ttcggctcac tgctcgtgag
     2461 ccttgccggc ggagagccgt tgcttcgcaa agatttgccg caaatcgtaa gtgccatctc
     2521 tgaatatcat ctgccgttca tcacgacaaa cggttcactg gttacgcagg aaaatgccgc
     2581 cgccctcatg gctgccggct tgtggggcgt ctcggtcagt atcgattacg cagacccaaa
     2641 aaaacacgac gacgccagaa atcgccaggg tgcatggcag cgcgcatggc aggcggttga
     2701 gtacttcagc aaggccagga cgcaggaatt tcagcgggtg aacgttatgg ccgtattgct
     2761 ggataacaat atcgacgagc tggaaaccat catgaagatg gcggctgagc gtaatgcgta
     2821 tttcatggtt cagccgtacg gctatctcaa aacgggttcg catgattacg aatgcagaca
     2881 cagcaacatt tcttcgcgtc tgctcgatat gtggcggcgc aaccggaatt ttctgtccaa
     2941 cccgacgtac ttgaaaaaat tcgatgcgtt tcacaatggc ggcatagagg gctgcaaggc
     3001 cggcagggcg ttttttaaca tcgattccac gggcgatatt gcaatctgcg ttgaaaacaa
     3061 gcatgcgcct atcgccaatc tttatacgga cagccaatat aagattctga acgcgctcaa
     3121 atccgcaagc cgccgcaata cctgcaaatg ctgctggtac aactgtcgcg gagaagtgga
     3181 aagcctctat aatttcaaga gtctcatcca atcattaccg accttctttt tcaatcacgg
     3241 cgttgcggac ggcggcaaga tgggccggtg gacgtaagct gcaaatcgcg aggctttgag
     3301 aaccgcgcta agattttgat tcggggtgtc tgtcttcata tggcacaggc gtgccggctt
     3361 tggcgaatac agccagatgc acttcgcttg cgccggcgtc aagcagggtc cttaccgctt
     3421 cgtcagccgt tgcaccagtg gttaccacat cgtccacaag cagtattttc aggccggaca
     3481 tgagttgcgg attgcggatt ttgaatgcgt ctttcacgtt ttccattctg cgcgagcggc
     3541 agaaatgcgc ctgttgcggc gtgttgcgta tacggatgat ttcccggctt gccggcaggt
     3601 tcagtttgcc tgctagacat tttgccatca tccacgagtg attgccgttg cgcaatattc
     3661 tgcgggccca gaacatcggc actggtgcga cgaggtcgaa ctgcgtctgc gaaacatttg
     3721 gaatgtgttc ggcaagcaat ctggtcagag gttcaatcat cgcccattgg ctgtgatatt
     3781 ttatctgccg tatgattggt tttagaactc cggcgtaagg cccgattctg taaacgcggc
     3841 gaaaccgcgg cattggcacg gggcagtcgt aacagccgct gttggctttg ctcgtaagat
     3901 gtgggccgag cgatgttccg caaaacggac agtatggcag tgtgacgcag ttgaggagtt
     3961 ttatgcggca ttcttcgcag agcattgaat gtccgactgg tttggcgccg caacttgcgc
     4021 agacgttcgg cacgaacatg tccagaattt cggcgcatat ccgtctgaca tgcaagagac
     4081 ttttttcgtc gcgataaatt tcactcgtct ggttcattgc tgcggcattc cgtaggctat
     4141 ggaatttcgt ccgcacgccc attgcatacg catgaatcgc atttgcagaa gtcttgtcga
     4201 ttcgtcggct gccgccgatg gcgttgtcag cgacggagcg agggtgttta ttacgggatt
     4261 catttccgtt acgacgaagg attccgcaac atcgttcagg tttgttgcgg cattgagcag
     4321 cagggagtcc agcacgattc tgtcgccggc aaacatgaga gcggtccaca cccgccgccg
     4381 gggaaagtcc ggaatccaca gaagattgcg aacctgctca tgcaggactt ctctgccggg
     4441 ccagagcgtc agaagtgcac attggcatga acctttggcg aaaaaatcct cgcgattgag
     4501 acgtcggtcg attcttgctt ccgcacttgc acgttcattt gccgagtcgg tcgcgaatcc
     4561 gagcagcagt gcgccggcgg ctcgtacgtc tggattaagt tctatgcgtt cgttttcgcc
     4621 tggcagcagc cattggccca gcgcataagc ctctgttgag tgcgtcgcgc cgatgttcca
     4681 cgcaatgtaa tcgcccgcga aagccggctc gccggtcacc gcctcccgta ccaggaatgc
     4741 cgtggtgtat tcgactttca tcgtctccat atcggggttg tcgccggatg aaatcaggaa
     4801 ctctatgtgt gccggactta tcagccatgc agccacagct gccgccgccg aagatttggg
     4861 cgtatttgtg aaatttccct gcctgtcctg acgaatccac gtagccgcct gtcgcaggag
     4921 ttcgagacac tgttcctgcg taggcgcgcc ggctggctga tttggggtca ctgcctgctc
     4981 acccagcaat ctcgcagccg gtatcagcat aagatgccgc cctggctgcc aatatgtttt
     5041 tatgaaatcg taaatttccc gccgacatgg tatgcccatc tggtcgagcg tctgcatggc
     5101 tgcgattatg tgggactgca tcatgccagc cgcctttttc gggtcgattg cccgaatgag
     5161 tatatccctt gcaatggcgg cggctatggg catattcatt tttgcgccgg ctgaaagcat
     5221 catggcgttg ggacattttt gagcggcgga gcattttttg aaatattcgg ctaacgcgtc
     5281 ggttatatcc tggtcggcaa tcagcggcag aatgaaactg agcttttccc tcaagatttc
     5341 atcgtcagtt tcaatcgcga gagaggcgat ttttttcaaa tccggcgttg tcgcctgcat
     5401 atttgcgagg gccattgcgc aggcggcaat caggtctggt tcaactcgac tggcacgtat
     5461 cgagcggttg aaaatcgcaa tgacgtcgtt caggcatgca gtacggtttg ccacgcttac
     5521 ggctatggtt gcttccgcgg ccacctgcgg gtcggaatct gcaagaagtt ttttcaatat
     5581 atcgtcgttg ccaagttctg cggcaagtgc agccgcggct tgacgcacgg ccggcagagg
     5641 gtcgacatga acaagttcaa gtgcgcggcg aacgtatcgg ttgtcgtcgc cgtttatgat
     5701 gaaatcgtat atgaatgtgc gacgtgacat tgcggcggct ttacggtttt cagccggctg
     5761 ggtctgcgga gatttgtcgg ctgcgagcag tttttccagt tcgataaggt tatagcggtc
     5821 cagccatacc ggctgaacgt gtttggacgt gaatttccgg aagtggaaaa gtatgtcatg
     5881 aattgcaatg aactttgcct tatcgggcgt gtcgagcgag gcgatgagtc tttttatcat
     5941 tgccggcctg ttgcttgcgc gggtcgccgc cgcaatagcc gcatcacgct gctgctggta
     6001 atctcctgca ccgagttttt ggattgccgc gtaatcccgc cacgccggca gagcgaatat
     6061 gccgattatg cacgcgatta tcagcagaat cagcagccgc aactcccacc tgagtattct
     6121 gtgatttcgg aaaaattttt taagaggaat tttttgcatt cgttattcct tttgtataat
     6181 tcgtttttct tattataatg accaaatcat caaaataaaa ggattaccgc atgaacgaaa
     6241 cccgaataga gcgtgattcc atgggtgaaa tgcctgttcc ggctgaagca ctttacggcc
     6301 ctcagacggc acgagcggtg gaaaattttc cggtttcagg ctgggctatg ccgcgcgagt
     6361 ttatcgccgc aatgggctgg ataaagcagg ctgccgccgc gacgcattgc gaagccggtc
     6421 gactcgacgg cgaactcgcc gacgctatca ttcaagccgc cggcgaagtc atttctggca
     6481 aactcgacgc acattttccc gttgacgttt ttcagaccgg cagcggcaca agcagcaaca
     6541 tgaacgtaaa tgaagtcatc gcacgccgcg cgtcgcagat tctcggcgga cgcaaagtgc
     6601 atccgaacga cgatgttaat cgcagccaga gttcaaatga cgtcgttccg actgcgatgc
     6661 acgtttcggc tgcggtggcc atgcatgaca gtcttctgcc ggcacttcgc gttttgaagg
     6721 aaatgctgga cataaaagcc ggcgaattcg acggcatagt aaaaatcggc cgtacacatc
     6781 ttatggatgc cgtgccggtg cggctgggcc aggaattttc gggttatgct tcgcagatag
     6841 aagctgccat agtcgtaacc caacaggcga tgggctcact gtgcgaactg cccatcggcg
     6901 gcacggctgt ggggacgggc ctgaatgcgc cggcggattt cgcacagtcg gtctgcctgt
     6961 acctcggcga ggaacttcat cttccgttcg tggagacttc aaatcatttt gcggcatcgg
     7021 ggtcgaggga tgcagccttg caggcatcct cggcgatgcg gcaggttgcg cttgcgatgg
     7081 gtaaaattgc aagcgatatc cgtctgctcg gcagcggtcc gcgatgcggt attggcgagc
     7141 tggaactgcc ggctgttcag cctggcagca gcataatgcc cggcaaggtc aatcccgtga
     7201 tttgcgaatc ggtaatacag gtggcttgcc aggttgtcgg ttgcgatgcg gctatagcgg
     7261 ctggcgtaac cggcggcgta ggtggaattc ttgacctgaa cctcgccatg ccgatgatta
     7321 cgatgaatct tataacgcag ataaaacttc tggcgaatgt agccagggta tttggtgaaa
     7381 aatgcctcgc cggccttaaa gccaacgtca gccgctgtgg tgaacttgtg gaaaaatcgt
     7441 tggctatggt gactgtgctg gcgccgatag tcggttatga taaggcggcg gagcttgcgc
     7501 acgaggcaat gaaaagcggc aaaaccatac gcgaactttg catggaaaag aaaattctgc
     7561 cggctgataa gttgacggaa cttcttaatc cggctgctca aaccggacag caatgaaatt
     7621 gaagcggttt tattgcggtt cttgaaaata acgcaagatg aaaactcgca acgagccctg
     7681 ttaataaagg cctgtacatc agaacattgc aagactttaa agaaccgctc tggaaaccat
     7741 atgcaattat tttcccgaac cgctctataa aaaataacga ttcgattgtt tgaaaaaaat
     7801 cagaaactgt ttgaatattc catttctttc agcggaatat cctcatccca tccgccgccg
     7861 agagctttgt ataacgatat cagatacaaa gacacgtttc ctgaactttg tatgagggag
     7921 ctttgcgtat ttaaaagtga ctgttgtgcg ctaagcacgt tcagaaaatc agtattgcct
     7981 tcagaataca acacagtcga tatctcgact gctttttggt tctggacgac agcctctttt
     8041 agcgccttga cgtgttcctg ttccatttgc gattgaatca gggccgtttc gacttcctgg
     8101 acggcattaa gcaccgtctg ccgataggtc agcagtgatt catccttcag tgccttttgc
     8161 acgtcgatat ttgcaagcgt tgcaccggat tggaaaatgt tccaactcat agatggcccg
     8221 aatgaccaca ggcgacttgc cggattgaac atggtgttca agtctgccga actccagccg
     8281 agtgaacttc cgaaattgaa ttttggaaac aggtcggcga cggctacgcc gatattggcg
     8341 gttgcggaat ggacatttgc ctccgccatg cgtatatcag gccgacgtct gagcaaatcg
     8401 gacggaatgc cgaccggcac tgccggcgga gcaatcggta ttgtcgcgga ttttgaaagc
     8461 cgctccagca gcgcagacgg ctcacgattc ataagaacgc tcagtgcgta tatcgcctgt
     8521 ctttccgatg attccagtag tggaatctgg gctgatgtcg ctgcgacctg ggcccgagag
     8581 ttggcgacgt caaggccgct gacgaaaccg ccttcaaatt ttttacgagt cacagcctca
     8641 ctacgctgct gtgcctgcag attctgtttt gttatgatta ttcgctgctg aagcgtgcga
     8701 agttcaatat aatatctggc gacctcggcg gcgagagtga cttggacgtc gcgaaggctt
     8761 tctacggagg cttgcagttg tgcttcggca gattcgatgt ttcgctgaac gccgccgaaa
     8821 atgtctattt cccatcctga gtcgaaaccg gcttgatact ggttgcttac aggggtattg
     8881 cctgactggc cgcgctgata gctgcttccg ccgtcgagtg ttgggcctat gcctgaaact
     8941 gctatcccgt aattgccccg agcctgtcgt atgcgggtct tggcgatgca tacgtcaagg
     9001 ttggcggcca tggcttcatt gatgagcgac gtcagctcgg cgtcgttgaa aattttccac
     9061 cactcgctga gatttacttt ttcgacagcc ggctgagttt gtggagtctg ctgagctttt
     9121 tcaatccagc cggcgggcat ctgcgtttcg ggcttggtaa aattggggcc gaccttgcag
     9181 ccgctcatga cggctgctac gcatgccatc gcgaaaatcg ccccaaatac acttgtcgat
     9241 agcatttttt gacaaaagag tttttgacga aagggcctgt aataatgcat ggcagaatcc
     9301 tttctccatt tctgtgccgg ctgatttgtg aaaaaatcac tcatatcgca atgcctcaat
     9361 cggatcgagt ttggctgctt tccatgcagg ataaaaaccg aaaataatgc ctatgccgac
     9421 tgccactgag acggctgaga ttattgcaaa agacgacgtt tcaaccggcc attttaggaa
     9481 aaattccaac agcagtgaac cgccgtggcc gagcagtatg ccgattgtac cgccaaccag
     9541 gcagagaatt accgattcgg tgaggaactg ctgaagaatg tcgcgggagc gtgccccgac
     9601 tgccatccgc aaccctatct ctcgcgtacg ctccgtaacc gacaccagca ttatgttcat
     9661 tatgccgacg ccgcctacta tcagcgaaat tgtagctacg caaagcagaa gattggtcat
     9721 caatttggtc gaagacgtca gcatgtcgag catttcagtt ctgttaaaaa cctcaaagtc
     9781 atttttatct ttgggctgaa tatcatgacg tctgcgaagt acggcggtta tttcatcagt
     9841 tgcctgttgt atatgatttg agtctatcac tgacgccatt atataatcta tacttttaaa
     9901 acgtgtcagc ataggattgt ttttagtctg tgtagatgat tgggacggat acagcgaggg
     9961 aagtttacac gggacaggat tacttaaatc tgtagatgtg ctctcgctgg aactgctttg
    10021 gctggaactt cctgatgatg tgccgttaac acgatattta accgttgtcc aaggtgctac
    10081 cactatatca tccaggtctc ctccaaacat atttgcgcct tttttgtcga gcacgccgac
    10141 tacgatgagc gggacgttgt ttattcttac ttccttgccg agtggatttt ttccgttaaa
    10201 aagttcatgg actactgttt ggcctattac acatattcgc gcggaggtct gaacatcacg
    10261 ttcagtaaag gttctgctcg tattaaagtt tcctccccac accataatct caaaataagc
    10321 tgcagttact ccccacatcc gtcgtggagt ccagttttta tttccgtaaa ctacaggtgc
    10381 tgtcgaccag cagacaatcg gtgttacggc acgaagcgat gggcattcct ttttcaatgc
    10441 ttcacaatca tccggcgtca acgacattac agagcctgag ccctggttta cgccactgct
    10501 ggaaattgag ccaggagata ttatcaacat gttggagccc atgttcacta tactttcccg
    10561 tacagccgaa gatgagcctt cgcctatttc catcatggcg attaccgacc ctattccgat
    10621 aactatgccc agcgtggtaa gcattgcccg cagaggattg cggcgaagcg tcttggtcgc
    10681 agtcgtcaaa gtacgataaa tcttcatttc gcacctcccg acgaagtgac tttatcttcg
    10741 tggcacacgg aaggtttttc agatttcggg caatcatcga caatcatgcc gtcgcgtatg
    10801 tgtatgaccc tgtctgccga atgtgcgacg tcggcatcat gggtgacgag tattatcgtc
    10861 aagccgtctt caacgttgag ttttctgaac atctgcagga cttcctggct cgtatgcgaa
    10921 tccaggtttc cggtaggttc gtcggcgaag agaatcggcg gatggtttat aagggcacgg
    10981 gcgatggcga cgcgctgctg ctggccgccg gataactgcg acggctcatg gtgcattcta
    11041 tgttccagcc ccactttgca gagtatttcg ctggcccgtt tgcgacattc gtgttcggaa
    11101 atgtgatgcc gtgtgtacgc aagcggcatt gctacgttat ccacagcact ggtacgggcg
    11161 agcagattga agctctggaa tacgaagccc aacttctggt tacgtatcaa cgctcgcgca
    11221 tcgggagata gtttggaaat ttcatggccg tccaaccaat attcgccggc tgtcggcctg
    11281 tcgaggcacc ccagaatatt catcagcgtc gttttgccag accccgacgc ccccatcaat
    11341 gccacaaatt cgccgcgaga tatttttaga gaaactcctt tcagcacagg cacttccatg
    11401 ttgcctatgc ggtatgtctt atatatctcg acaagttcca gaaattccat atattttatt
    11461 tctctagcag ggctaacaga ggctatcgtt tccgtttgga actgaagggc aggaacggat
    11521 ttacataatc gtccgatttt tcctcaacag gtttcatgcc gattacgacc tgcatatgtt
    11581 cctgcaaatc cttgccggaa acttcggcat aggcttcatt tgtaaggccg accgaaactt
    11641 ccacaggttt tacgtattcg ccgtctttta cccaaagaat atcttgagtt gtttttatgg
    11701 ttttgtcggc tggttgcgat gaaggttggg ttgcagtggt tttcgcttct gcggctggtt
    11761 gcgactcggc tactttataa tcccgatatt cctgcgcaat ctgttccttt ttgggattcc
    11821 atgacagtgc ggctaagggt accgtcaata cattttcccg ttttgagacg atgaaccgta
    11881 catttgccgt cagatagggt tttagcttgc ccttaaaatt gtccgtgacg atttcaacgg
    11941 tgaaagtgac aatattttgc gtcatcgccg cgttgggcct tattttccag acttttccct
    12001 caaatgtttc atccggcagg gcatcaaccg tgaaggttac tggttggtct ttgtatattt
    12061 ttgtgatatc ggcttcattt accagaaccc agacctgcat gcgtctcagg tcgcgggcta
    12121 tcaggaagag gcttggcgtg ttcatgctgg cggtcacagt ctggccgata ctgactcgcc
    12181 ggtctattat catgcctgtg ataggtgatt ttattgtaca atattcaaga ttccgctggg
    12241 cgcgccagag gcttttttct ttcagggtga gagtggcttc tgcctggagg attgtagcct
    12301 tgccgacatc gacgttagct ttggctgcgt cataagtagc ttgatacgcg tcatagctcg
    12361 ccttggctaa tgcgtcagat gggcccagcc gttgtgcccg ttcccagtcg cgctttgcct
    12421 ggaggaattt ggcttgcagt tggcccagat tggctttttc cagctgaacg ccagccttgg
    12481 cggatgatac atttgcctga gcttccgctt cgtcggctag atacagaaca tcgtctattt
    12541 tggcaatcac cgaatctttt tcgacaatcg acccgtaatc tatagttttg ccattcttat
    12601 ctttgccgaa cgaaagaacc ctgccgctta tctgagcgcc tacgtctacg acttcttcag
    12661 gctcaagcgt gccgctggcg gcgatggaaa tggaaatatc gtcgcgagtt acatccacag
    12721 tgtcgaaggt cattttttct tcggcttcgc cctgcattat ccatccgaca atcccggcaa
    12781 ttatagcgag aatcacaatc gcaaatatag atttccggac tatatgtttc atactttacc
    12841 tcatcattta tgtatgtgga aagccggttt caaaacctga ttctgttata gaggaacttc
    12901 tattagctga tatcgtcgga agacaggaat aacgttaaaa tttatattat ttacttaact
    12961 ctaattgccg cacgacacta tattgaattc aattattaat ttagtgtcaa tggaaaagtt
    13021 atcaaaaggt tgtataatat tatcatgatt taatattaac agccgcgata atatatcaaa
    13081 agccatcccc aatacatggg cggttgttga aatagagcaa ttttgcgggt ctggtaagta
    13141 agaaaaatgg cggttatttt aaggatatta tatctttttt gacgccgcca tggctgcaaa
    13201 agagccgatt gcaggcagtc aagtttttta gacaggttct aaaatcagag attgaattca
    13261 ggagtaaccg gaaaatgtta tcgtttatca caattaatcc gcctggaagt tttagcactg
    13321 taccaatcag acagtgggta gccggaatca atcttgaagg aagatgtcat gaataccatc
    13381 cggatggaga tttttcgcag gaatgctatg aatttggagt catcaaacct aattttccgc
    13441 accattggga ggtaccggcg gacggcattc catggaagag tttacatttc ttttttacgc
    13501 cgtccgtatc catgctgccg cttttacaat ttgatgaaat aaggcctgga ttcatgaaac
    13561 tgccgctggc ggagtcgcct tttttataca aggtgcagcg aatgttgttt aagagccatt
    13621 atcttttgaa cagccattgg ttaaattgtg tcgatttggc tatgaatgcg ctggaaggcg
    13681 ctttgctttg gtgccgggct gaagttgaac gtgtacaaca aaagacagat tcccgcattc
    13741 tcaccgccat tgaatatatg aatcgtcata ttgccgaaaa aatttatctt gaagacattg
    13801 ccaaggcatc gtttttatct gttcccagat taatgtctct ttttaaggcg aatataggcc
    13861 tctcgccgat ggcgtatctt gatgacgagc ggatggagcg tgccagcaag atgctgacga
    13921 cagggtattt cagcatcaaa gaaatagcag ccatgaccgg atatccggat ccagcctatt
    13981 tcagtaaacg ttttttgcag catttcagga tgtctccgct tgcctacagg aaaaagcata
    14041 ttactgatgt ttagcttggt actgttttct gatgccttat aatatggtcg gcgttcgaga
    14101 acccctctga atttgcggaa tattccgtaa acttttctta aagattagcg cttattccta
    14161 ccgatactga atttaatgga taattcgtgt atttttctgc gcttaagtga gcgttgtaag
    14221 gatagccgta aaggaacgcc atgaaaacca gaatgattat tataacattg ctggctggtg
    14281 gttgcattat tggatgtgta gaccctggca aatacgacgt taacgacgtg tataaacttc
    14341 agcagtccat aatggacaga agccctcaga accgttctga tagcggcaca gggccgatga
    14401 taccctcaga aaattccgtg ccacggataa agatggaaaa gaatcccaag accaatcgct
    14461 atgaggtcag cctttctctt cgtgagacgg tcatgcgggc gctggcgaac aatcctcaga
    14521 tagcggttgt gagctttact cctcagattt caagagaact ggtggtacag gccgcctcgc
    14581 aattcgacta taccattttt ggcggatacg aatttacgca cacggaaagt tcgaatacaa
    14641 cgactaccgg caatattacc ggcattccta ctggcaaact caatgccaac gatttccagt
    14701 tcggcgtaca gcagcagacg ataacaggtg cgcaatggag tctggcggcg tctatgattt
    14761 ctacgcgata tcgtcctact acgaacgact gggattatga caacagcatc gcgttcaacg
    14821 tcactcagcc gctgcttcgc ggggctggca cggaagtcaa tctggctaat cttcggatag
    14881 cgcggctgaa ctacaaaatt tcgatgagcc agttcaacca gaacgtccag cagacggtcg
    14941 tgtcagttat gagtgcctat tacaatctca tcaaggctcg ccaacttgtt gtcattgccg
    15001 aaaatcttct cgccgtgacc gagcagacct atcgcaaagt tagcgaacga catagccttg
    15061 atgccacaaa ggtgcagata aagcagtctg aaaccgcggt tcgcgaacgg caggccgcgc
    15121 ttgtacttgc tcgcaaaaac gcacgcgacg cacaggatgc cctgcttgtg ctgcttggag
    15181 acgaacagct taacctgctg ggcagaaaca tggacataat accggctacg actcttacgt
    15241 ctctgccggt tctgattgat ccgacagacc agtatctgac ggcattgcgg ctcaatccgc
    15301 agttggagca gttgcggctc gccgtcagcc agagcgacat caacataatg gttgcggaaa
    15361 atcaagcgtt accgtcattg aaccttactg ccggagcaac gctcgcaggc gacagcagcg
    15421 gcacgaatcg cggcgcgtgg aacggcatgc ttgacgccag atacaccagc tacaatgccg
    15481 ccctaatgtt tgaatatcct ctcggcaacc gtcagcgatt aagcgcagtt cggcagagca
    15541 agttgcaacg caggcaggct atttcgaacc tgcaaagcca atccgaccag cttgcccagc
    15601 agatacgtga aagcatccgc cagataaaca ctcaatatga agaaataaag atacgccggc
    15661 aagcactcga tgcggctaag gagcagcttg acgcccttga cgtgcttgag aaaatacgtg
    15721 ccaagctgac ccctgagttc ctgaatctca aactgaacgc ccaggcgacc gtggcgacgt
    15781 cggaaagcaa tctgattgac gcccaggtac tctataatgt ggcgatgctg aatctgtcca
    15841 gatatactgg tacggtactt gagttgaacc aggttcagtt atcgctgcct gtcgtgactc
    15901 agccggataa ttcggctaat ccgctgacgc ttacgccggc tccgccgaac gatgcgcctg
    15961 tgccgctacc gtcgatgctg caaagcagca gttcaaaata aacggctatt ccacaagcat
    16021 gtctttttgg gtattttggc gcgcttatta tacgttattg tttcacgagc cggagtatgc
    16081 gcggttgcat tgttgccact ccggggcgtt tttgaggcgg tatagcattt cgccggcttt
    16141 tggaacgtag agcttacctt cgctttcatg gccttgccat tgggagatgt ctatgcttgc
    16201 cggattgcga taattcatgc cgatgcggcg gcaggtttct tcgggtatgc cggtcgccag
    16261 tgttacggtc actcgcggca cttcacggcc atttatccat ttgccgatgc cgcgaacatg
    16321 tgtcgaatga gccagaactc cccatggata atccttatac ctctcccact ggttgatgaa
    16381 gaacggtagc gaatgataac cgacttcctc tataattctg ccgtgcgtca ccgcgatttc
    16441 gtggatatgc ggcgcatata ttataagttc gccgccgtcg gcgactacgc attccatctt
    16501 gtacatgcat ttgccgccag tccagagttc atcatacatc agtggcgcac gggacaggat
    16561 tgaatcgtat gccctgtccg tatatagaat atgcagtttg tcggacaggt ctgcggcggc
    16621 tgaccaggac gacttgacat cg
//
LOCUS       DAONFI010000007        15097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_4249_length_15097_cov_4.658490, whole genome shotgun sequence.
ACCESSION   DAONFI010000007 DAONFI010000000
VERSION     DAONFI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_15097_cov_4.658490"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /gene="ndk"
                     /locus_tag="PLK08_00505"
     CDS             complement(<1..364)
                     /gene="ndk"
                     /locus_tag="PLK08_00505"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPS52010.1"
                     /translation="MEKTLVLVKPDGVQRGLIGEVIRRLENKGLKIVAMRMLQVDEAL
                     ARKHYAAHVNKPFFPGLLTFITACPIVAMVLEGPSAVAVVRNMMGPTNGAEAPLGTIR
                     GDYGISKQFNLVHGSDSPE"
     gene            complement(553..2178)
                     /gene="xseA"
                     /locus_tag="PLK08_00510"
     CDS             complement(553..2178)
                     /gene="xseA"
                     /locus_tag="PLK08_00510"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPS52011.1"
                     /translation="MAAKQRNKLFDFTQAQGPSDEPHVGEFTRSPASANKDKKTIGVS
                     ELVERIRGALTAAMPGRLAVVGEISNLSRPASGHLYFNLKDESASIGACMWKSSAGKL
                     KFSPADGMEVVVTGKVDVYGPQGKLQLYVESISPRGEGALELAFRQLKEKLSAAGLFD
                     PTHKRTIPQFPRAVGVITSATGAAVRDIQRTIRRRWPGMNVYLLPATVQGEGAAAQLA
                     TAVTALDSAADKFDIDVIIIGRGGGSMEDLWPFNEEILARAIFAAHTPIISGVGHEVD
                     FTICDMVADLRAATPTAAAEHAVPDREEIVRHVGQLQSAMTRSTSQRIRAARLAVDGI
                     ARSVFFRDPLYSVKARTQRMDELSARLASAQQGLLSRANKRLEPMSHRLAALHPSRLC
                     ERAKAKLDNVFSRLRWVLGAHSKHAGDRLAEIASRLTAARPQIKLAMARQHVTSLERQ
                     LAAMSYRNVLMRGFSVTRGQDGNILRSTSAVTAGMRIETEFFDGKISSTTDGDTVSPV
                     KSAGNSQPSSKAPRKTPAKKRQSPTTDQPGLFD"
     gene            2308..2556
                     /locus_tag="PLK08_00515"
     CDS             2308..2556
                     /locus_tag="PLK08_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPS52012.1"
                     /translation="MEQCTTSQPECPPVCKSERTRLAAALLCVFLGCLGIHRFYVGKI
                     GTGIAMLLTGGLFGVWTLVDFIMILTGSFKDCDGKVLS"
     gene            complement(2642..3217)
                     /locus_tag="PLK08_00520"
     CDS             complement(2642..3217)
                     /locus_tag="PLK08_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPS52013.1"
                     /translation="MAGIKGSKTEQNLLKAFAGESQARNRYTYFASAAAKEGYEQIAA
                     IFQETADNEKEHAKRFFKFLEGGPLEITATYPAGKISDTLENLKEAAAGENEEHTVLY
                     PGFGDVAKKEGFAEAADAFYNIAKVEVEHEKRYRKLIENIEKNQVFKKTQPVRWKCRN
                     CGYVHEGTEAPDKCPSCLHAKKFFEIKETNY"
     gene            3740..3812
                     /locus_tag="PLK08_00525"
     tRNA            3740..3812
                     /locus_tag="PLK08_00525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3773..3775,aa:Thr,seq:ggt)
     gene            4248..5453
                     /gene="tuf"
                     /locus_tag="PLK08_00530"
     CDS             4248..5453
                     /gene="tuf"
                     /locus_tag="PLK08_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPS52014.1"
                     /translation="MAKEKFNRTKPHVNVGTIGHVDHGKTTVTSAITIVQGAKGLAKV
                     VSYDEVAKASESQGRRDATKILTIATSHVEYESNNRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAPDGPMPQTKEHVLLARQVNVPRLVVFLNKCDLMEDEELLELVEMEIR
                     ELLDKYDFDGDNTPIIRGSATGAIADPNGPGAECIQKLMDALDTWIPEPVREIDKPFL
                     MPIEDVFSIKGRGTVVTGRIEKGIVKVGDKVQIVGLGDTRETTITGVEMFNKEMDQGQ
                     AGDNVGCLLRGIEKVDVERGMVLCAPGSITPHHKFEAEVYVLSKEEGGRHTPFFTGYR
                     PQFYFRTTDVTGTVALPEGVEMCMPGDNVTMTIELLNTAIAMEEGLRFAIREGGRTVA
                     SGVVTKILE"
     gene            5465..5632
                     /gene="rpmG"
                     /locus_tag="PLK08_00535"
     CDS             5465..5632
                     /gene="rpmG"
                     /locus_tag="PLK08_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPS52015.1"
                     /translation="MAKSKAREYVWLQCSECKDLNYRTNVRVQGGVPKLELSKFCPRD
                     RKHTLHKIKRK"
     assembly_gap    5758..5857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5864..5936
                     /locus_tag="PLK08_00540"
     tRNA            5864..5936
                     /locus_tag="PLK08_00540"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5897..5899,aa:Trp,seq:cca)
     gene            6018..6416
                     /gene="secE"
                     /locus_tag="PLK08_00545"
     CDS             6018..6416
                     /gene="secE"
                     /locus_tag="PLK08_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPS52016.1"
                     /translation="MQGLNIYKKSQGRYARWLTFFGVMLVGAWGCVTLTQTLGAYPNV
                     SVYVQFILPALILLGLAWVMFKYVVNRPSTADFLIATEGEMKKVSWSSTKEVIGSTKV
                     VIVTTFLLAALLFLVDFVFVNLFSVIGITG"
     gene            6432..7058
                     /gene="nusG"
                     /locus_tag="PLK08_00550"
     CDS             6432..7058
                     /gene="nusG"
                     /locus_tag="PLK08_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPS52017.1"
                     /translation="MTELDNTNAEQASENQGGQPLASDPSMRWCVLRVASNKEEQVRD
                     ALVRKVKIEGLDGQIGRILVPTERRPSPRGKAGKQKFVDRKMYPGYVFIEMKLQDDGS
                     IEENTWFTIKETNGVGDFIGSGGKPSPMADEDVAKMLMQVELAKEGSAVTVEFAKGDV
                     VKIKEGAFENFEGEIDEVLPEKGLVRVVVTIFGRSTPVELEFWQIEKI"
     gene            7323..7748
                     /gene="rplK"
                     /locus_tag="PLK08_00555"
     CDS             7323..7748
                     /gene="rplK"
                     /locus_tag="PLK08_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPS52018.1"
                     /translation="MAKEIKAKIKLQAPAGKATPAPPIGPALGAKNVNIGQFVSQFND
                     RSKEMTPGMPLPVEITVYADNSFTFIIKSPPAAALLKEAAGIESGSGVPNKTKVGKVT
                     KQQIRKISEAKFKDLNARDLDAADRIIAGTARSMGIDVE"
     gene            7913..8599
                     /gene="rplA"
                     /locus_tag="PLK08_00560"
     CDS             7913..8599
                     /gene="rplA"
                     /locus_tag="PLK08_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPS52019.1"
                     /translation="MAKVCHGKRYRQCLEKVPSAPVSVADAVKALKTFAVPKFDMTVD
                     LVMHLGIDPKQADQQLRGSISLPHGIGKSKKVIAFCEGSELEAAKKAGAVEVGGDDLI
                     KKITDGWMDFDVAIATPAMMKSVSKLGRVLGPSGKMPSPKAGTVAQDVAKAVAEFAAG
                     KIEYRNDDFGNVHVPVGKMSFDEKVLVENIEFFIDYVKRHKPSTSKGTYIKKISVSGT
                     MTPGIAIAAN"
     gene            8615..9133
                     /gene="rplJ"
                     /locus_tag="PLK08_00565"
     CDS             8615..9133
                     /gene="rplJ"
                     /locus_tag="PLK08_00565"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPS52020.1"
                     /translation="MSKPVKTLIKQELAKRFDEVTSVAICGFTGLDSGSTYAMRTRLA
                     EKGIKVTVVKNSLAKAAFKEAGLDIATDLLEGPCAVAYGADSIVSVVRELLAINKDMP
                     AFTVKGAILDGDVFSGDEQVKALSKFPTREEALSQLVGCILSAGSNISGALMGPAGQI
                     AGILSSIEQKGE"
     gene            9338..9727
                     /gene="rplL"
                     /locus_tag="PLK08_00570"
     CDS             9338..9727
                     /gene="rplL"
                     /locus_tag="PLK08_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011747091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPS52021.1"
                     /translation="MSEEAIKELGDKIAALTLKEAVALKDYLKEAYGIEPAAGGAVMM
                     AAPAAGAGAAEAAEEKTSFNVVLKEAGANKIQVIKVVRALTNLGLKEAKDLVEGAPKT
                     VKENASKDEAAEAVKQLEEAGAKAVVE"
     gene            10518..14276
                     /gene="rpoB"
                     /locus_tag="PLK08_00575"
     CDS             10518..14276
                     /gene="rpoB"
                     /locus_tag="PLK08_00575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPS52022.1"
                     /translation="MLKRDSVRNFCKVGDFAPLPNLIDLQTASYERFLQADIEPSKRK
                     NEGLEALLREVFPMISYDETMRLDYLDYELGEPRFKPQECRDLRLTYGLPLRIRCRLV
                     CKDPKIDIEESIYVGEIPIMLGGGEFIINGAERVIVSQLHRSPGVDFTVELQESDRPL
                     HGARIIPERGSWIELSVTKKDSITMRIDQSSKIPATMFMRSLDERYGATQAIIREFYA
                     VEKVAAEKLTARTYLVEGYVDPETGEELVPAGGQVGDAMNQIVASAKKKIEIIAGTPD
                     PLILNTLSEDPSTDYESALLRIYARLRPGNPPQVEKAKALFQEKFFDENRYRLGKVGR
                     FRINRKFGQDVPESVMSLRVEDFVHTMSYMLKLRGGQGVIDDIDHLGNRRLRTIDELA
                     ADEMRKGFLKLRRTVQERMSLKDPSEITKIAEIVNSKSISSSIEFFFGRGELSQVVDQ
                     QNPLAQLTHERRLSALGPGGLNRKRAGFEVRDVHISHYGRICPIETPEGNNIGLISSM
                     GIYAGVDDYGFLTTPYLAVDNRKVLGEVVNLRADEEMMTTLAPPEVLLNANRRGETTY
                     LPEGRVLARVDGELSTAEAKNVKYIDISPKQTVGISASLIPFLEHDDANRALMGSNMQ
                     RQAVPLLRTDPPIVATGMEKAIAENTSFSIKARKAGKVTYADAERIIIDGSEEYELRK
                     FEKLNERACQNQKTIVKVGERVKKGQIIADGSATYHGELALGRNVLVAFMPFDGYNFE
                     DAILLSEKLVREDVFTSVHLEDYEVEIRETKLGREEFTRDIPNVSEKSLRYLDANGVV
                     QVGTRVKPGDILVGKVSPKSKTELSPEEKLLHAIFGRAGEDVKNDSLELPAGEEGVVV
                     STKHFSRRGPHMTEEQKKQLKKDMKDYEDKMNERMAKIFREMCGEINEVAGQAMIDPT
                     TRQKVGQSENLAVILEQIETFNAKWIKGEKEKGEEVYNRYWQRIKDIEKEKQRKLAHM
                     KRGDDLPSGVLEMVKVTVATKRKLGVGDKMAGRHGNKGVIARIMPEADMPFLEDGTPV
                     EILLNPLGVPGRMNLGQILETHLGWAAAVLGFQAVTPIFDGATEGEIFKAIDEANQHV
                     RERREMLDVVQTAGDTAELLAEIPLDGKVRLRDGRTGEYFEQKTTVGYIYMNKLHHLV
                     DEKIHARATGPYSLITQQPLGGKARTGGQRFGEMEVWGLEAYGASYTLQELLTVKSDD
                     VEGRTKIYESMVKGQNTLEAGTPVSFDVLCNELRGLGLNIHMEKKKLL"
     gene            14374..>15097
                     /locus_tag="PLK08_00580"
     CDS             14374..>15097
                     /locus_tag="PLK08_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPS52023.1"
                     /translation="MAESVYDRINDYGSVKIMLASPNDIRSWSFGEVKKPETINYRTY
                     RPEKDGLFCERIFGPERDWECACGKYKGTKHKGIICDRCGVKVTHSRVRRKRMGHINL
                     AAPVVHIWFFKALPSRLGTLLDMRTADLEKIILFQDYVVTDAGQTPLKFQQVLTEEEY
                     RAARDKYGMDFRATMGAEAIAELLRKISMDDLAKTLREDLQKTSSKQKVKDIAKRLKL
                     VESLRTSGNNPEWLVMEVIPVIP"
BASE COUNT         3751 a         3662 c         4125 g         3459 t
ORIGIN      
        1 cttccggcga gtccgacccg tggacgaggt tgaactgctt ggatattccg taatcgccgc
       61 ggatagttcc cagcggtgct tctgcgccgt ttgtggggcc catcatgtta cggacgactg
      121 cgacggctga tgggccttcg agcaccatcg ccactatcgg gcaggcggtt atgaacgtca
      181 gaaggccggg gaaaaacggt ttattcacgt gtgccgcata gtgcttgcgg gcgagagcct
      241 cgtctacctg cagcattcgc atcgcaacga tttttagccc cttgttttca aggcggcgga
      301 tgacctcgcc tatgaggcct ctctgcacac cgtcgggttt taccagtacc agcgtttttt
      361 ccatgttgtt aagactccac cccgttgaat gcaattttta tcatttttat aactctctgc
      421 cccacgggtg atgttttatt cgcccacgcc ggaaatatca agcaaaacct gccctgcgtg
      481 caggaatttt caagtcacaa gactcaagtt acaagcaaat ttgaaaatcg aaatttatca
      541 gtcaaaaaat cgtcaatcaa aaagtccggg ttggtcggtg gtgggggatt ggcgtttttt
      601 ggcgggggtt ttgcgtgggg ctttgctaga tggttggctg ttgccggctg attttacagg
      661 ggagacggta tcgccgtcgg tggtgctgct gatttttccg tcgaaaaatt ccgtttcaat
      721 tcgcatgccg gctgtcactg ccgatgtgct tcgtaaaata tttccgtcct gcccgcgggt
      781 tacgctgaat ccgcgcataa ggacattgcg ataactcatt gccgccaact gccgttccag
      841 cgatgttaca tgctgccgcg ccatggccag ttttatttgt ggccgcgctg ccgtcaggcg
      901 gcttgcgatt tcagccagcc tgtcgcctgc atgcttgctg tgtgcgccga gcacccatct
      961 cagccgcgaa aaaacattat ccaacttcgc tttcgcccgc tcgcacaaac gcgacggatg
     1021 cagcgctgcc agacggtgcg acatcggttc gagtctttta tttgcccgtg acagcagtcc
     1081 ttgctgcgcc gacgccagcc gcgccgaaag ttcatccatc cgctgcgtgc gtgccttgac
     1141 cgaataaagt gggtcgcgaa agaaaacgct ccgcgcgatg ccgtcgactg ctagccgcgc
     1201 ggcgcggatg cgttgcgacg tcgaacgcgt cattgccgac tgaagctggc cgacgtgtcg
     1261 cacgatttct tcgcggtcag gcacggcgtg ttccgccgca gccgtcggcg tagcggcgcg
     1321 gaggtcggct accatgtcgc atatcgtgaa gtccacctcg tggccgacgc cggagattat
     1381 cggcgtgtga gcggcaaaaa tcgcgcgagc cagtatttct tcgttgaacg gccagaggtc
     1441 ttccatgctg ccgccgccgc ggccgatgat gatgacgtcg atgtcgaatt tatcagccgc
     1501 cgagtccagt gcggttacgg cggttgcgag ttgtgcggct gcgccttcgc cctgtaccgt
     1561 cgcaggcagc aggtacacgt tcatgcccgg ccaacgtcgg cggatggtgc gctggatgtc
     1621 gcggacggcg gcgcccgtcg cactggttat tacgccgacc gcacgcggga attgcgggat
     1681 ggttctttta tgcgtcgggt cgaataggcc ggcggcggag agtttttcct tgagctggcg
     1741 gaatgccagt tccaatgcgc cctcgccgcg cggcgaaatg ctctcgacgt aaagttgcag
     1801 cttgccctga gggccgtaaa cgtccacctt gccggtcacg accacttcca tgccgtcggc
     1861 tggcgaaaac ttgagcttgc cggctgacga tttccacatg cacgcgccta tggaggcgga
     1921 ttcgtctttt aaattgaaat acaaatgacc tgaggcggga cgtgacaggt tgctgatttc
     1981 accgacgacg gctaatcggc ctggcatcgc agccgtaagt gcgccgcgaa tacgctccac
     2041 aagctccgat accccgatgg tttttttatc tttattggcg gatgcgggcg aacgggtgaa
     2101 ctcgccgacg tgcggttcat cgctcggccc ttgtgcctga gtgaagtcga acagtttgtt
     2161 gcgttgtttg gccgccatgc gttttaagat aaccgaatgg gccgaaaagc tcaagtatcg
     2221 gccgacaaag ttgaattttc atgtctgcgg gaataaaata taagtgtagg ggtaatcgaa
     2281 atctatattt tttaaggaga aatcgaaatg gaacagtgca cgacatctca gccggaatgc
     2341 ccgccagtgt gtaaaagtga aagaaccaga cttgctgccg ctttgttgtg tgtgtttttg
     2401 ggatgcttgg gaatccacag gttttacgtc ggcaaaatag gtacaggcat tgcaatgctg
     2461 ctgaccggcg gactttttgg agtatggacg ctggtggatt tcatcatgat actgacaggg
     2521 tcgttcaaag attgtgacgg caaagttctt tcgtagtaca aagcgtaaaa tgtgaaggcg
     2581 caaaaaaaga ccggcggtta gccggccttt tttttgctca attctgcgca agtaaagcca
     2641 atcaataatt ggtctccttg atttcaaaga atttctttgc gtgcaggcat gacgggcatt
     2701 tgtccggcgc ttccgtgcct tcatggacgt aaccgcagtt acggcatttc cagcggacag
     2761 gctgggtttt tttgaaaacc tggtttttct cgatgttttc gataagtttt ctatagcgtt
     2821 tttcgtgttc cacttcaact ttggcgatgt tgtagaacgc atctgccgct tccgcaaaac
     2881 cttctttctt tgccacgtcg ccgaagccag ggtaaagaac ggtgtgttct tcgttttcgc
     2941 cggcggcggc ttctttcaga ttttccaggg tatcgctgat tttgccggca ggatatgtgg
     3001 ccgtgatttc cagagggccg ccttcaagga acttgaaaaa ccgtttggcg tgttcctttt
     3061 cattgtcagc cgtttcctgg aatatggctg ctatctgttc ataaccttcc tttgccgctg
     3121 ctgacgcaaa atatgtgtaa cgattgcgag cctgcgactc gcccgcaaat gcttttaaca
     3181 aattctgttc agtcttcgaa cctttaatgc cagccatgag tatttctcct taaatgatac
     3241 tgagttaagg ttgttaatgt caaaaaagtc ctgattttcg atatactgca caattatagg
     3301 cccacgacaa ggaaaattct gcaatgaagc caatttttta aatatgtcat cgcagatttt
     3361 cgccttgacg tacacgcgcc ttgcatcata ttttttaaaa acaaatcttg gaacatgctc
     3421 taacatgccg gaattcggct attcttgtcc gtaaaaattt ttaaataaaa tctataagcg
     3481 tgcaactctt tttattgaaa agagttatgg ttttgggttg tagttttaag ggtacatcgg
     3541 atgagagaac gtttatttca gtgaaaaaat tttaaaaatt tttttggaaa atccataaag
     3601 cattatttga caatagcggg cgaaaatgta tattttatcg cttcccgacg gattggggcg
     3661 cagaatatct gcgcacatat acattttagc gagacgatat tttgagcacg cggctgagct
     3721 gacgtgcatg ttggtatttg ctgctgtagc tcagtcggta gagcgcttcc ttggtaagga
     3781 cgaggtcatg ggttcgaatc ccatcagcag ctttgccgtg tggaacggct cggcggctgc
     3841 aggatagcgt ttgttggcgg cttatggacg agcggcggtt tgtgcggcag tggtgcactt
     3901 tttcgccgct ttgttgtttt gtggcggcaa aaaggtccat aatgattttt gaatgttttt
     3961 gagtggttca aatattcgaa ccatttttga aatattttaa aatattttga aatattaaag
     4021 acagcccgac aggggcttgt ctggatgata aacaagcgat ttggtacttt tgaaatgacc
     4081 ctagccgaga tgtccggtgc ggtatcgggc ggaaaggcaa tttttaaagg tcagctaaaa
     4141 aagaaggtgg catgcccggg tgaggcatga cctggcgata cgagagagta tcaaaaagcc
     4201 tttaagtgaa acatagttta tataggtttt catcaggagt aacaggaatg gccaaggaaa
     4261 agtttaacag gacgaaaccg cacgtcaacg tcggcactat cggccacgtc gaccacggca
     4321 aaacgacggt aacctcagcg ataaccatcg tacagggcgc gaaaggcctt gctaaggttg
     4381 tcagctacga tgaagtggcg aaggcttccg aaagccaggg ccgccgcgac gcaaccaaaa
     4441 ttctgacgat tgcgacctcg cacgttgagt acgagtcgaa caatcgccat tatgctcacg
     4501 ttgactgccc gggccacgct gactacgtca agaacatgat taccggtgcc gcccagatgg
     4561 atggcgccat tctcgtggtt tcggctcctg acggcccgat gccccagacc aaagaacacg
     4621 tcctgcttgc ccgccaggtt aacgttcccc gcctggttgt attcctcaac aagtgcgacc
     4681 tgatggaaga cgaagaactc ctcgaactcg tcgagatgga aattcgtgaa ctcctcgaca
     4741 agtacgactt cgacggcgac aatactccga ttatccgcgg cagtgccacc ggcgcaatcg
     4801 ccgaccccaa tggccctggt gcggaatgca tccagaaact tatggacgca ctcgatacat
     4861 ggatacccga acctgttcgc gaaattgaca agcctttcct tatgcccatc gaagacgtgt
     4921 tttccataaa aggccgcggc actgtcgtta ctggccgtat cgaaaagggc atcgtcaagg
     4981 ttggcgacaa ggttcagatt gtcggtctgg gcgatacgcg cgagaccacc attaccggcg
     5041 ttgaaatgtt caacaaggaa atggatcagg gccaagccgg tgacaacgtc ggctgcctcc
     5101 tccgcggtat tgaaaaggtt gacgtggaac gcggcatggt tctctgtgca ccaggcagca
     5161 taaccccgca tcacaagttt gaagccgaag tttacgtcct gagcaaggaa gaaggcggcc
     5221 gtcatacgcc gttcttcacc ggctatcgtc cgcagttcta tttccgcacc acagacgtga
     5281 ccggcacggt tgctctgccc gaaggcgtgg aaatgtgcat gcccggcgat aacgtcacca
     5341 tgaccatcga attgctcaac acggcaatcg ccatggaaga aggccttcgt ttcgctatcc
     5401 gcgaaggcgg ccgtaccgtt gcgtccggcg ttgtgacgaa aattcttgaa taagaggtgt
     5461 aacaatggcc aaatccaagg cccgtgaata cgtatggctg cagtgctccg aatgcaaaga
     5521 cctgaactac cggacgaacg ttcgcgttca gggcggcgtg ccgaagttgg aactcagcaa
     5581 gttctgccct cgtgaccgca agcatacgct gcacaaaatc aaacgtaagt aatgaaaacc
     5641 gataggctct ggagcggaac gtagtttttg atgtagccgc cgtaattttg cggtttttaa
     5701 ccgtaaatga aggcagcagc cgtcaggcgg caggcgaaag cctgataaat tatgatgnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc tatacggcag tagctcaatt
     5881 ggcagagcag cggtctccaa aaccgcaggt tgggggttcg actcctccct gccgtgtagc
     5941 gaagaaccgt aacagccgtg accgcaggta gtatcgccaa gttacagacg ttcggataat
     6001 tttgaggata tacgtaagtg caaggattga acatatataa gaagtcgcag ggccgatatg
     6061 cccgctggct gacttttttt ggcgtaatgc tggtcggcgc atggggttgc gtgacactca
     6121 cccagactct gggcgcatat cccaacgtca gcgtatacgt gcagttcata ctgccggcgc
     6181 tgattctgct tgggctggcg tgggttatgt tcaaatatgt cgtaaatagg ccgtcgacgg
     6241 ctgatttcct catagcaacc gaaggcgaaa tgaaaaaagt gtcatggtcc agcacgaagg
     6301 aagtgatagg ttcgacgaaa gtcgttatag ttaccacttt tcttctggcg gctttactgt
     6361 ttctggttga cttcgttttc gtcaatctct tttcggtgat tggcattact ggctgataaa
     6421 ggcggacgaa tatgactgaa ctcgataaca ccaatgcgga acaggcgtct gaaaaccagg
     6481 ggggtcagcc gttggcttct gacccgtcga tgagatggtg cgttcttcgt gttgcaagca
     6541 acaaggaaga gcaggtgcgc gacgcgcttg tgcgcaaggt gaaaattgaa gggctggacg
     6601 gccagatagg ccgcatcctc gttccgaccg aacgcaggcc cagcccgcgc ggcaaagctg
     6661 gaaaacagaa attcgtagac cgcaagatgt atcctggtta cgtgtttatc gaaatgaaac
     6721 tccaggatga cgggagcatt gaagaaaaca cgtggtttac gataaaggaa accaacggag
     6781 tcggtgattt tatcggctct ggcggcaagc ccagcccgat ggcggacgaa gacgtcgcca
     6841 aaatgctcat gcaggttgag ctggcgaaag aaggttcggc cgtcactgtt gagttcgcca
     6901 agggcgacgt ggtgaagata aaagaaggcg cgttcgaaaa ctttgaaggc gaaatcgacg
     6961 aagttttgcc ggaaaaaggc cttgttcgtg tggtcgtgac aatctttggc cgttctacgc
     7021 cggtggaact ggaattctgg cagatagaga agatatgaaa tttatcagcc acgccggagt
     7081 ttggcggagg ctgattggaa ttttaaatag gcgatgcgat gaaccagata gtcgcgtcgg
     7141 ctaaaaaatt gagtattgtt cgatttttgt gatttgatgc ttgtaggttt tgttcagttg
     7201 ccagcctgtt tgaaattgga aaaagcggaa tcgggtaact ggctgacaag cgataaaaat
     7261 gattacgcac tgtttataag gcttggtggc ctgaataaac atttttgata gagagacgca
     7321 agatggcaaa agaaattaaa gcaaagataa agttgcaggc gcctgccggc aaggctacgc
     7381 ccgcaccgcc gattggccct gctcttggcg caaaaaacgt caatatcggc caattcgttt
     7441 cgcagtttaa cgaccgttcg aaagaaatga cccccggtat gccgctgccg gttgagataa
     7501 ccgtgtacgc cgataattcg ttcacgttta tcatcaaaag tccgccggct gccgcgcttc
     7561 tcaaagaggc ggcgggtatc gagagcggtt caggcgtgcc gaacaagaca aaagtcggca
     7621 aggtcacgaa acagcagatt cgcaaaatat cagaagcgaa gttcaaagac ctcaacgccc
     7681 gcgaccttga cgcagccgac cggattatcg ccggcactgc gaggtctatg ggtattgatg
     7741 tcgaatgacg atatgtaaaa atctgccgac tgtggcagta cgtcaaacca cgggcgttaa
     7801 agtcgtggga gtcttggacg gtttttaaat tttttaaaag attttttaaa gaaaaattta
     7861 aaaaaattta aaaagttgaa aacgttcgca atgaagacgc aggagtagaa caatggcaaa
     7921 agtgtgccat ggaaaacgtt accgccagtg tctggaaaaa gtgccgtcgg cgcccgtatc
     7981 ggtcgcagat gcggtcaagg cgttaaaaac cttcgccgtg ccgaagttcg acatgactgt
     8041 agacctggtg atgcacctgg gtatcgatcc caagcaggct gatcagcagc ttcgcgggtc
     8101 aatatccctg ccgcacggca ttggcaagag caagaaggtt atcgccttct gcgaaggctc
     8161 ggaacttgag gcggcaaaga aggctggcgc tgtggaagtc ggaggcgacg accttatcaa
     8221 gaaaataacg gacggatgga tggattttga cgtggccatc gctacgccgg cgatgatgaa
     8281 gagcgtttcc aagcttggcc gtgttcttgg gccgagcggc aaaatgcctt cgccgaaggc
     8341 tggtacggtt gcgcaggacg tggccaaggc tgtggctgag ttcgcagccg gcaaaatcga
     8401 gtatcgcaat gacgatttcg gcaacgttca tgtgccggtc ggcaagatga gctttgacga
     8461 aaaggtgctt gttgaaaata tcgaattttt catcgactat gtaaaacgtc acaagccgtc
     8521 aacgtccaaa ggcacttaca tcaagaaaat ctcggtatcg ggaacgatga cgcccggtat
     8581 tgcgattgca gcaaattaaa caggcaggtg agccatgagc aaaccggtaa aaacacttat
     8641 aaagcaggaa ctcgccaagc gtttcgacga agtaacgtcc gtagccatct gcggctttac
     8701 tggcctcgat tcaggttcga cctacgcaat gcgaacccgt cttgcagaaa aaggcattaa
     8761 ggtaacagta gtcaagaatt cattggccaa agccgccttc aaggaggctg gcctcgatat
     8821 tgcgactgac cttctggaag gcccttgcgc agtagcctac ggcgccgaca gcatagtcag
     8881 cgttgttcgc gaacttcttg caatcaacaa ggatatgccg gcgtttaccg tcaagggcgc
     8941 gattcttgac ggcgacgtgt tcagcggcga cgagcaggtt aaggcgttga gcaagttccc
     9001 gacgcgggaa gaggctctga gccagctggt cggctgcata ctttccgccg gctcgaacat
     9061 ttccggcgcc ttgatgggcc cggccggcca gatagccggc attctctcgt ccatagaaca
     9121 aaaaggcgag tgatgatttt tgttgagcgt cggagttatt cggcgtgtgt ttggagcggc
     9181 tctcaaaaga attttttttt gagaatcggc attggaagaa ataattttcg gctgcggatt
     9241 gggcctgact gctcgggctt aaatgggacc agcggggttc cgcctatcaa gccggcattt
     9301 tttggactag tttcaactag tcagtggagt ttttgaaatg agcgaagaag caatcaagga
     9361 attgggcgat aaaatcgccg cactgaccct caaggaagcc gttgccctca aggattacct
     9421 caaggaagca tacggcattg aacccgcagc tggcggtgca gtcatgatgg ctgcccctgc
     9481 agccggcgcc ggcgctgctg aagctgccga agaaaaaacc agcttcaacg tagtcctcaa
     9541 agaggcaggc gccaacaaga tacaggttat caaagtcgtt cgcgcattga ccaacctcgg
     9601 cctcaaggaa gccaaagacc tcgtcgaagg cgcccccaag accgtcaagg aaaatgccag
     9661 caaggacgaa gccgccgaag ccgtcaagca gcttgaagag gctggcgcaa aagctgtggt
     9721 tgaataattt tttaaatttt ttaaaaagtt aaaaaaaatt aaaaaatgtc aaccttcctg
     9781 tttggcctgc tttttacggc tgaatgggat atgaaacatt gtgccttccg ccctgaataa
     9841 tcggggcgga aggtactttg tctcttgcag ttgcctggct aaccaaggcc gggcagatgg
     9901 ttatttcgga atttttttgg tttttggttg ttttttaaaa aaactcaaag tgacagattg
     9961 acaatattta cctttacacc taagtatact ggaaaccgtt aaatcagtca gtttgaataa
    10021 aaaatataaa aaatttaaaa atatttttaa aaattattcc gaaatgacta ctaaaaagga
    10081 tattataagg attctcgaaa aattgcggaa ccttataaaa atgacagatg cgtttaaatg
    10141 gctggccagg ttttacgggc ttgtaatgcc agaaacggat ttgtcaaagg cttatactcg
    10201 tatccggcag actggaagtc tgtgggattc gggtatatgt gtatttcggg attcgacaaa
    10261 aaaaccgaat tgcacataac gccgagattt tgcaacagtt aaggcggtta gccggttgct
    10321 atcgaaatga atttgtaagt tggccgtgct tggactgtct caaaattttg agcgacataa
    10381 gctgtaacgt gacaggaaat tccgtggcct tgagccacaa cagtttagtg aactaaatcc
    10441 ggattaggac gtgacgccgg cgataacgca tcgcctaagt catgcccgtt atcgctagca
    10501 gagaggtaaa aaagcatatg cttaaaaggg actctgtaag gaacttctgt aaagttggtg
    10561 atttcgcacc tctgccgaac ttaatcgact tgcagacagc cagttacgaa agattcctcc
    10621 aggcagacat tgagccgtca aaacgtaaaa acgaaggcct tgaagcgctt ctgagggaag
    10681 tattcccaat gataagctac gacgagacca tgcgtcttga ctatctcgat tacgagcttg
    10741 gcgaacctcg cttcaagccg caggaatgtc gcgatttgcg tctgacctac ggcctgccgc
    10801 ttcgcatccg ttgccgactt gtctgcaagg atccgaagat tgacatcgaa gagagcattt
    10861 acgtcggcga aattccgata atgcttggcg gcggtgagtt tatcataaac ggtgccgagc
    10921 gggttatcgt ttcgcagctt caccgcagcc ctggcgtcga cttcacggtc gagcttcagg
    10981 agtctgaccg ccctctgcac ggcgcgagaa ttatccccga acgcgggagc tggatagagc
    11041 tttctgtgac caagaaagac tccatcacga tgcgaataga tcagtccagc aagatacccg
    11101 ccacgatgtt catgcgttcg ctggatgagc gatacggcgc tacccaagcc atcattcgcg
    11161 aattttacgc tgtggaaaag gtagccgcgg aaaaactcac cgcccgcaca tatctggttg
    11221 aaggctacgt tgatcctgaa acaggcgagg aactcgtacc cgccggcggc caggtcggcg
    11281 atgccatgaa ccagatagtt gcgtcggcta aaaagaagat tgaaatcatc gcgggtacgc
    11341 ctgacccgct gattctgaac actctttcgg aagacccgtc gacggattat gagtcggcgc
    11401 tgcttcgcat ttatgcgagg ttgcggccgg gcaatccgcc gcaggtggaa aaggccaaag
    11461 ccctcttcca ggaaaagttt tttgacgaaa atcgctaccg tctgggcaag gtggggcgtt
    11521 tccgcataaa ccgcaaattc ggccaggatg tgccggaatc cgtcatgagc cttcgcgtgg
    11581 aagatttcgt tcatacgatg tcgtacatgc tcaagttgcg tggcggccag ggcgtaattg
    11641 acgatatcga tcacctgggc aatcgtcgtc ttcgcaccat cgatgagttg gctgccgacg
    11701 aaatgcgaaa gggcttcctg aagctccgcc gtacagtcca ggagcgcatg agcctcaaag
    11761 accccagcga aataaccaaa atcgccgaga tagttaattc caagagcata tccagcagca
    11821 tagagttctt cttcggccgt ggcgaactga gccaggtcgt agaccagcag aacccattgg
    11881 cgcagcttac gcatgagcgt cgtctgtcgg cgttggggcc tggcgggttg aatcgcaaac
    11941 gtgccggctt tgaagtccgc gacgttcaca tctcgcatta cggccgaatc tgtccgattg
    12001 agacgccgga aggcaacaac atcggcctta taagctcgat gggcatttat gcgggtgtag
    12061 acgattacgg ctttttgact acgccgtatc ttgccgttga taaccgcaag gtgcttggcg
    12121 aagtagtcaa tcttcgggct gacgaagaaa tgatgaccac tcttgctccg ccggaagtgc
    12181 tgctcaatgc caatcgtcgc ggcgaaacca cctatctgcc cgaaggcagg gtacttgccc
    12241 gcgttgacgg cgaactaagc acggcggaag cgaagaatgt gaaatatatc gatatttcgc
    12301 ccaagcagac tgtcggcatt tcagcgtcgc tgattccgtt ccttgagcat gacgacgcca
    12361 accgtgcact gatgggttcg aacatgcagc ggcaggctgt gccgttgttg cgcaccgatc
    12421 cgccgatagt ggcgacgggc atggaaaagg ctatcgccga gaataccagc ttcagcataa
    12481 aagcccgcaa agccggcaag gttacatacg ccgacgccga acgcatcatc atcgacggca
    12541 gtgaagaata cgaactccgc aagttcgaaa aactcaatga acgtgcctgc cagaaccaga
    12601 aaaccatagt caaagttggc gaacgggtca agaaaggcca gattatcgcc gacggttcag
    12661 ccacgtatca tggcgagctg gcgctgggcc gcaatgttct cgtggcgttc atgccgtttg
    12721 acggttataa cttcgaagac gccatcctcc tgagtgaaaa actcgttcgt gaggatgttt
    12781 tcacgtcggt gcatcttgaa gattatgaag ttgaaatacg tgagaccaaa ctcggccgcg
    12841 aagagttcac ccgcgacatt ccaaacgttt cggaaaaatc gctgcgatat ctcgacgcca
    12901 acggcgtggt gcaggtcggt acgcgagtaa agcccggcga catactcgtc ggcaaggttt
    12961 cgcccaagag caagaccgag ctcagccccg aagagaaact tctgcatgcc atctttggcc
    13021 gagccggcga agacgtcaag aacgattcgc ttgaactgcc tgccggcgaa gaaggcgtcg
    13081 tggtctccac gaagcatttc agccgccgtg gcccgcacat gaccgaagag cagaaaaaac
    13141 agctcaagaa ggacatgaag gactacgaag acaagatgaa cgaacgcatg gccaagattt
    13201 tccgcgagat gtgcggcgaa atcaatgaag ttgccggcca ggcaatgatt gaccccacga
    13261 cccgtcagaa agtcggccag agtgaaaacc tcgccgttat tcttgagcag atagaaacct
    13321 ttaatgccaa gtggattaaa ggcgaaaagg aaaaaggcga agaggtctac aaccgttact
    13381 ggcagcgcat caaggacatc gagaaagaaa agcagcgcaa gctggcgcat atgaagcgcg
    13441 gcgacgatct gccgagcggc gttcttgaga tggtcaaggt taccgtagcc accaagcgca
    13501 agctgggcgt cggcgacaag atggctggcc gccacggcaa caagggcgtt atcgcccgta
    13561 taatgccgga agcggatatg ccgttccttg aagacggcac gcctgttgaa atcctgctca
    13621 acccgctggg cgttcccggc cgtatgaacc tcggccagat attggagacg cacctcggct
    13681 gggcggcggc ggttctgggc ttccaggctg ttacgccgat atttgacggc gcgacggaag
    13741 gcgagatatt taaggctatt gacgaagcca accagcacgt ccgcgaacgt cgtgaaatgc
    13801 tcgacgtcgt tcaaaccgcc ggcgataccg ctgaacttct tgcggaaata ccgcttgacg
    13861 gcaaagtccg cctccgcgac ggccgaaccg gcgaatattt cgaacagaaa acgaccgtcg
    13921 gttacattta catgaataaa ttgcatcacc ttgtcgatga aaagattcat gcccgcgcaa
    13981 ccggccctta ttcgctgatt acgcagcagc ctctgggcgg caaggctcgt acaggcggcc
    14041 aacgcttcgg cgaaatggaa gtctggggcc tggaagcata tggcgcaagt tatacgttgc
    14101 aggagcttct taccgtcaag agcgacgacg tggaaggccg tacgaagatt tacgaatcca
    14161 tggtcaaggg ccagaatacc ctcgaagccg gcacgccggt tagctttgac gttctgtgca
    14221 acgaacttcg cggtttgggc ctgaacatcc acatggaaaa gaaaaaactt ctctgacgcg
    14281 ggttgcttaa tgcagcctgt ttggagaagg cggctggttg tgattactgc ttgttttgat
    14341 taatgcaaac ctccttgcta ggagcaaaaa gatatggctg aaagcgtata cgacaggatt
    14401 aacgattacg gttcggtgaa aattatgctc gccagcccga acgatatccg cagttggtcg
    14461 ttcggtgaag tcaaaaagcc cgaaaccatc aactacagaa cgtaccgccc tgaaaaggac
    14521 ggccttttct gcgagaggat attcgggccg gagcgcgact gggagtgtgc gtgcggcaaa
    14581 tacaagggca cgaagcacaa gggcatcata tgcgaccgct gcggcgtaaa ggtcacgcac
    14641 agccgcgtgc gccgaaaacg catgggccac atcaatcttg ccgcgccggt cgtacatata
    14701 tggttcttca aggcgctgcc cagccgtctc ggcacattgc tggatatgcg cacggcggac
    14761 ctggagaaaa ttatcctttt ccaggattac gtggtgacgg atgccggcca gacgccgctg
    14821 aagtttcagc aggtgctgac cgaagaggaa tatcgcgccg cccgtgacaa gtacggcatg
    14881 gatttccgtg ctacgatggg cgcagaagca atcgcggaac tgcttcgcaa aataagtatg
    14941 gacgacctgg ccaaaaccct ccgcgaagac ctccagaaga cgagttccaa gcagaaggtc
    15001 aaagatatcg ccaaacggct taagctagtc gagagtctcc gtaccagcgg caacaatccc
    15061 gaatggctgg tcatggaagt gattccggtg attccga
//
LOCUS       DAONFI010000008        14987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_4301_length_14987_cov_3.737276, whole genome shotgun sequence.
ACCESSION   DAONFI010000008 DAONFI010000000
VERSION     DAONFI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4301_length_14987_cov_3.737276"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLK08_00585"
     CDS             complement(<1..447)
                     /locus_tag="PLK08_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009637744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPS52024.1"
                     /translation="MAKEQKIRKIVLAYSGGLDTSVILPWLKNRFNCEVVAFVADVGQ
                     GLEELDGIKQKALNTGACAVEVADLRKEFAEDYCFKMLKSGAIYEKNYYLGTSIARPL
                     IARAQVACARKHKADAVSHGATGKGNDQVRFELTFMALAPDLKIIAP"
     gene            complement(567..1577)
                     /locus_tag="PLK08_00590"
     CDS             complement(567..1577)
                     /locus_tag="PLK08_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52025.1"
                     /translation="MSDVAPKIPQLRMLFWESTARCNLSCIHCRRTETDAKNELSTAE
                     ARAFFKDVSSFSRPVIVFSGGEPLMRNDWRELAEYAGTLNLPTALATNGTLIDDSIAK
                     DIKKAGFRRVAVSIDGPDSPSHDGFRGQNGAFLKALVGLDKLAKAGVERQINITVAAH
                     NVERLDEIYNIARRLDVAALHLFMLVPVGCGIKIEQSHQLNAERYEKTLNWICDRQLS
                     GEMELRATCAPHYMRVAMLRGIPVSGRGCLCGISVAFVSHRGDVFPCGYLPLKCGNVR
                     RKPLGEIWRTSEHFAALRNYDRLTGKCGACMFKEYCGGCRARAFAENGDYLSVEPFCT
                     YE"
     gene            complement(1570..2727)
                     /locus_tag="PLK08_00595"
     CDS             complement(1570..2727)
                     /locus_tag="PLK08_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52026.1"
                     /translation="MVGISKILCSTSEPGDVIRYTESSSAGPAVVVFNCTRQCNLSCR
                     HCYASAGNSAGGDGELTTDGIIRLIDELADIGTSVILFSGGEPLMRDDIFDIVAHAGR
                     RNLHAVLSTNGTLITPQTAKRFADIGLDYIGVSIDAAQPEANDDFRRWNGAFDMACQG
                     IINARNAGIRCGLRFTMTRQNISHIPAIFSMAENLQINRICFYHLVPVGRAGASLSPD
                     SWHIRRGLDEIITHTQRLHDAGKAMEVLTVDNHADGAYLYLKLLASGRKQHAELALKL
                     LKKAGGNASGRKICCVSWDGDVYPDQFWRNRPLGNINHKNFNEIWHDETCDSLLRQLR
                     DRHSLLNGRCKKCRFLDICNGNLRARAEHAGNGIWGDDTACYLTDKEIAHV"
     gene            complement(2734..3807)
                     /locus_tag="PLK08_00600"
     CDS             complement(2734..3807)
                     /locus_tag="PLK08_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS52027.1"
                     /translation="MRKSVGKKLLTFWAVTAIFVVGGCEMNMDWWKQFQLKKEQPSVT
                     KEAASNAYVENSRQCIRNGELENALDELERAIQVNPSNSEAYTTIGDIYRHKGEYIKA
                     CNSYKTACIADQYAFRPHYNLGVTYQILATMAEGVKQARIYLNKAVHTYIRATVIKPD
                     SFDAHLNLGACYFQLGQFEQANEETAKANKLDSDNVKAINNLGIIAEMQGDAEKAISY
                     YKTSIEYDPNQVNILLNLASLYTRTGKYRAARSTYEAARKIDENNPATWQQIGVFCYR
                     IKYYQKAIKAFQYAIHLDPQSPGAYRGLGVVCMTVYIQDPKRTDLYANAIAAWKFSLQ
                     LQPKQPDLEALLERYEQPNVANR"
     gene            3921..4301
                     /gene="rnpA"
                     /locus_tag="PLK08_00605"
     CDS             3921..4301
                     /gene="rnpA"
                     /locus_tag="PLK08_00605"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPS52028.1"
                     /translation="MSMENGRLQFRRRLRLSAKRDIDAVFDRGSRAGNAVLTVIALQN
                     PANRDVSRLCAAVSRRNGGAVMRNRLKRLIREAWRLNRQDFPAGYDIVVLPRPGVKDE
                     LELFAAALRTLVPKAAARAGRQQG"
     gene            4298..4555
                     /gene="yidD"
                     /locus_tag="PLK08_00610"
     CDS             4298..4555
                     /gene="yidD"
                     /locus_tag="PLK08_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPS52029.1"
                     /translation="MSGEKTNPAGRALIFVVRMYQATLSPFLGRQCRFQPTCSNYFIE
                     AVQTHGSIRGSLLGLWRILRCNPLSKGGYDPVPVKKTKNTK"
     gene            complement(4681..5706)
                     /locus_tag="PLK08_00615"
     CDS             complement(4681..5706)
                     /locus_tag="PLK08_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPS52030.1"
                     /translation="MNAKNEKYSKYALCREMLDTVDVVRKFDPSRTAAVAAAIKANGR
                     LMLTGEGSSRIFPAKNFIRKVLTAGLNLQVVTEGSRQAMTYDLSRFAVFAASNSGRTK
                     EVVKLLKQLRDGGNKELFGLTAQPENLLKEYARETIVLNCGWETAVAATKSVIEQALT
                     YQSMLGHMTGSNACTCDCKNRLADAIEQALTIEIPADIVAAAAKAPTIYFAGYNDGVA
                     EELTLKTNEITRKKSDFLEGTYACHGIEEVMSPADIVFVVDPIAEEMDKFREVLVDGV
                     GLKVVCIATSPIAGFPTIVVPESGEGTPHVFLAAGWNILVEIGLALGINLDKPQRARK
                     VGNEFMG"
     gene            complement(5870..7072)
                     /locus_tag="PLK08_00620"
     CDS             complement(5870..7072)
                     /locus_tag="PLK08_00620"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPS52031.1"
                     /translation="MLKGNIIIGQSGGPTSVINSSLAGVIDAGRKAANVGKILGMRWA
                     IEGFMAGNVVDLSAEPQETVNLLRRTPSSALGSSRHKVKDPDFPVILQRLKEFDIRAI
                     FMAGGNDTMDTIHRVTEYANSHGYEMCGIGIPKTVDNDLFGTDHTPGYASAARYTALS
                     VQQAGRLARDMQKVDQFVIYQSIGRDAGWLSCAAALTRKNPADAPHLIYIPERVFDKA
                     KFLADVKSTHEKYGFVSIVCGEGIKYADGTPVSATKTKDKFGNEEFGAMGGSSVALNL
                     HRMIKDEFGWRGEFQITESLPMSAIDRAVGQDLDEAYACGCKAVELADSGKTGVMVSI
                     VRKSDSPYAVSYETVPLKDVAVKAKPMPDSFINAEGNFPSQAFFDYIRPLVGELDEYA
                     ELKYIPAK"
     gene            7240..8007
                     /locus_tag="PLK08_00625"
     CDS             7240..8007
                     /locus_tag="PLK08_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HPS52032.1"
                     /translation="MNTKQNSKRILYAGDTSLTTAAAYLAGIMTHYGRDFDYLASNQP
                     LADAPHQHGYALYIISDYPVNNWTDKEFKNVADDVEQGAGLIMLGGWESYCGLGGNYQ
                     NTPIADILPVKISPFDDRVNSPVPCLIEVCRKHPIIDALPFDRPPAIGGYNRVTLKPG
                     SQEILSLRPIDIIATGGGYDFAVGTAEPLLVTGCWGDGRVACLTTDVAPHWVGTLVDW
                     GMKRITAKAPHADSIEVGENYAKFFNRIIGWTLGEKL"
     gene            8421..>8664
                     /locus_tag="PLK08_00630"
     CDS             8421..>8664
                     /locus_tag="PLK08_00630"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HPS52033.1"
                     /translation="MTMIAEDLCAEQMLKPVMTTLQDRIGPQKFNAWFKHGTEVAFGD
                     DHIKVLVPNPFVANWIEHHYQSDLVESFFQHTRRRLP"
     assembly_gap    8665..8674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8675..9819
                     /gene="dnaA"
                     /locus_tag="PLK08_00635"
     CDS             <8675..9819
                     /gene="dnaA"
                     /locus_tag="PLK08_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPS52034.1"
                     /translation="SKRDFDMQAEIVKRTTQGRVRPRKLPANQQLKYRLRDFVVGPSN
                     YLAYQAALNAANPDNRNFTQLFVHGPCGVGKTHLLQGICNELASAQTENGRPLRWRYV
                     TGEQFTNEFIAAVRTQKAGEFREKYRSLDLLAIDDVHFLAAKRATQDEFLHTFNAIES
                     AGRQIILASDSHPRLVGDLNEGLVSRFLAGMVVKVDSPDKDTRLEILRRKAAGLKLAL
                     SPEVADYIAVHIRGSVRELEGTAVKLSAIAALQEEPINLTLVREALSDHLARTDSATT
                     LGDIEAVVAPFFGVTPADIHSSRRTKTVSAARMVAMLLARRYTPMSFPEIGRFMGKNH
                     SSVVLAVQRIEQSINKDENIDWNTPAGVKSMPAKTILQLLEEQLNY"
     gene            10342..11133
                     /locus_tag="PLK08_00640"
     CDS             10342..11133
                     /locus_tag="PLK08_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPS52035.1"
                     /translation="MAELQIKLEGVCLSRGGVEILHDIAWEVLAGQRWVLLGPNGSGK
                     TSMLEVITGYEWPNVGRVTLLDERLGEVDIHTHRRRIGLASTGIDHFIKKHQTALQIV
                     LSGFFDSTGLFDSPTTRQIHTAQTMLDMLGCGYLASRRFSLLSLGEQRKVLIARSLVY
                     SPEMLILDEPCAGLDIAAREFVLKTIAELAGNGHSAALSSIIYVTHHIDEILPCFTHI
                     LAIKSGGIVAAGPIRQTLTSDLMTELYGIPIEVGYFGGRYTARVR"
     gene            complement(11247..11319)
                     /locus_tag="PLK08_00645"
     tRNA            complement(11247..11319)
                     /locus_tag="PLK08_00645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11284..11286),aa:Ala,seq:ggc)
     gene            complement(11466..12344)
                     /gene="ispE"
                     /locus_tag="PLK08_00650"
     CDS             complement(11466..12344)
                     /gene="ispE"
                     /locus_tag="PLK08_00650"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPS52036.1"
                     /translation="MTDNRTANEASQSALEHNAPAKINLNLLVGPVGDDGFHPIDSVA
                     AKVSLFDRVCVTPRNDGEITCSVSGAIDCGPLEKNLAYRAAALFREINDCPGVDIAIH
                     KKIPTGGGLGGGSSDCATVLMAMNDMFGSPMSRESLAAAGGRLGSDVPFFMYDGCCRM
                     TGRGDNITPLAVSSFHAVLMFPGFPCPTGEVYCSYDQNPQTLEGQLDDEIWQSPCGLW
                     ESLLVNYLEEGAFRLAPELGLLQSRLKVTTDKLIHMSGSGSTLFSIFETRDAAEVFAR
                     LVNEEFSLPCEIASSF"
     gene            complement(12341..12526)
                     /locus_tag="PLK08_00655"
     CDS             complement(12341..12526)
                     /locus_tag="PLK08_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52037.1"
                     /translation="MRCYRPSYFRMPRIMQENSLIREKQANLEKYIHNVEDGVPIFTP
                     EESRDTCRFFSKDGRDK"
     gene            complement(12732..13013)
                     /locus_tag="PLK08_00660"
     CDS             complement(12732..13013)
                     /locus_tag="PLK08_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52038.1"
                     /translation="MLIGLGLDNQDGHIRITKGKNFHLMGGSRETHEEMQEKCIKFNE
                     KLDVRGKQLEDLEKKEFFELACECRMNVVAASPHKQPDGQIRKNPRRDG"
     gene            13222..14526
                     /locus_tag="PLK08_00665"
     CDS             13222..14526
                     /locus_tag="PLK08_00665"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPS52039.1"
                     /translation="MESNNITVVGLQWGDEGKGKIVDALARTSCYVTRYCGGANAGHT
                     VKAGGEKFALHLIPCGILHEKVINVIGNGVAFDPEVAWGEIVALRERHIRIDSENFRI
                     SSCAQLVMPWHKYQDKLNEQSLGRNKIGTTARGIGPCYADKANRSTSIRACDLLDVAS
                     LEQKIRHIGQQKNTVFAALYNAAPMDIGTIVAQFRSLAENLSPMICNTSALLRNACQA
                     GRRILFEGGQGSMLDIDHGTYPYVTSSPVTACGVPAGAGVSPKSVGRVLGIMKAYTTR
                     VGAGPFPTELDNEIGQAIRDAGHEYGTTTGRPRRCGWLDAMVVRYTASLSGVDELALM
                     LLDVLTGFDELKICTGYKIDGEIVKDFDPARLDEVECVYETMPGWQQDITDCRKFSEL
                     PAEAQNYVRRISEIVGVPVGIISVGPERTQTILHESEIKELA"
     gene            14523..>14987
                     /gene="uppS"
                     /locus_tag="PLK08_00670"
     CDS             14523..>14987
                     /gene="uppS"
                     /locus_tag="PLK08_00670"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPS52040.1"
                     /translation="MMSGQQLEKRFYEARQEIGCDPMKFPESIAIIMDGNGRWAQMRG
                     LPRPFGHKEGAKVVRQIVKEAARLGLKALTLYSFSHQNWSRPAEEVDALMHLFSEYLA
                     HERETVMENNIRLRHLGSRQGLPDMVLHELDETVRQSSKNTGMFLCLALNYGS"
BASE COUNT         3433 a         3837 c         3966 g         3741 t
ORIGIN      
        1 cggcgcgata attttcaggt ccggcgccag agccatgaac gtcagttcga accgcacctg
       61 gtcattgcct ttgcctgttg cgccgtgcga gacggcgtca gccttgtgtt tgcgggcgca
      121 ggcgacctga gccctggcga tgagcggcct cgcgatgctt gtgccgagat agtagttttt
      181 ttcgtatatc gcaccgcttt tgagcatttt gaagcagtag tcttcggcga attccttgcg
      241 aaggtcagcc acttcaacgg cgcacgcgcc ggtattaagt gccttttgct taatgccgtc
      301 gagttcctcc aaaccctggc cgacgtcggc gacgaacgcc accacttcgc aattaaagcg
      361 gttcttcagc cacggcagaa tgacggacgt atcgagtccg ccagaatatg ccagtacgat
      421 ttttctgatt ttctgttcct ttgccattct ttcttatctc cgcaatttat tccactcgga
      481 tgccgtttta tcggtacggc attcacattt tttgatagac ggccaaagcc ggtattctat
      541 caatttcctt catcaatgcg agtaatttat tcatacgtgc agaacggttc gacggaaaga
      601 taatcgccgt tttctgcaaa cgctcttgcg cggcagccgc cgcagtattc cttgaacatg
      661 caggctccgc atttgccggt gagcctgtca taatttcgca aagcggcaaa atgttcgctg
      721 gtgcgccata tttcgcctag cggtttacgg cgaacgttgc cgcacttgag cggcagataa
      781 ccgcacggga acacgtcgcc tctgtgcgat acgaaagcta ccgaaattcc gcaaaggcag
      841 ccacgtccgg ataccggaat gccgcggagc atcgcaaccc gcatatagtg cggcgcgcag
      901 gtagcccgca gttccatttc ccccgaaagc tgccggtcgc atatccagtt gagcgttttt
      961 tcataacgtt cggcgttgag ttggtgcgat tgctcgattt ttatgccgca gccgaccggc
     1021 acgagcatga acaggtgcag cgcagctacg tcaagacggc gggcgatgtt gtatatttcg
     1081 tccagccgtt cgacgttgtg tgccgcgact gtaatattta tctgccgctc gacgccggcc
     1141 ttcgccagtt tgtccaaccc tacgagggct ttgagaaatg cgccgttctg gccgcggaag
     1201 ccgtcgtgac ttggcgaatc ggggccgtct atgctgaccg caacccgacg aaaaccggct
     1261 ttttttatgt ctttcgcgat tgagtcgtcg ataagcgtac cgttcgtcgc cagcgcggta
     1321 ggcaggttca gtgttcctgc gtactccgcc agttcacgcc agtcattgcg cataagcggt
     1381 tcgccgccgg aaaagacgat gaccggccgg ctgaaagatg aaacgtcttt aaaaaatgcc
     1441 ctggcttcag ccgtcgaaag ttcatttttt gcgtcggttt cagttcgccg gcaatgaatg
     1501 caggaaagat tgcagcgggc ggtggattcc cagaaaagca ttcgcagttg cggaattttt
     1561 ggggcgacgt cagacatgtg ctatctcttt gtcggtcaga tagcaggcgg tatcgtcgcc
     1621 ccatatgccg ttgccggcat gttcagccct tgcacgaaga ttgccgttgc atatatcaag
     1681 aaatctgcat tttttgcagc ggccgttcag cagggaatgc cggtccctca actgccgcag
     1741 cagcgaatcg caggtttcgt cgtgccatat ttcattaaaa ttcttatggt tgatgttgcc
     1801 caatggccta tttcgccaga actggtcagg atagacgtcg ccgtcccaac tgacgcagca
     1861 tatttttctg ccgctggcgt tgccgccggc ttttttgagc agttttaacg ccagttccgc
     1921 atgctgcttg cggccggatg ctaacaattt gagataaagg tatgcgccgt cggcatgatt
     1981 gtcgaccgtg agaacttcca tcgccttgcc cgcgtcgtgc aaacgctgcg tatgagtgat
     2041 tatttcatcc aatccacgcc gtatgtgcca ggaatccggc gacagtgatg cgccggctct
     2101 gccgaccgga acgaggtggt aaaaacatat gcggtttatc tgcaaatttt cagccattga
     2161 aaaaatggcg ggaatatggc tgatattctg tcgcgtcatg gtaaaacgca gcccgcagcg
     2221 tatgccggcg ttgcgggcgt tgatgatgcc ctggcaggcc atgtcgaagg cgccgttcca
     2281 gcgacggaaa tcgtcgttag cctccggctg ggcggcatct atgcttacgc cgatataatc
     2341 aaggccgatg tcagcgaacc gcttggccgt ctgcggcgtg atgagcgtgc cgttcgtact
     2401 cagaaccgca tggagattgc ggcggccggc gtgtgcgaca atatcaaaaa tatcatcccg
     2461 cataagcggt tcgccgccgg aaaaaagtat caccgacgtg ccgatatcgg ccagctcgtc
     2521 aatgagtctg ataattccgt ccgttgtgag ttcgccatcg ccgccggctg aattgccggc
     2581 tgatgcgtaa cagtggcggc acgacaggtt gcactgccgc gtgcagttga ataccacaac
     2641 agcagggccg gctgacgatg attccgtata tcgaatcaca tcgcccggct cactggtaga
     2701 gcagagtatt ttggaaattc ctaccatcag gcgttaccga ttagctacat tcggctgttc
     2761 atatctttcg agcagtgctt ccaggtcggg ttgtttgggc tgaagttgca acgaaaattt
     2821 ccacgcggca attgcgttgg catacaggtc ggttcgtttg gggtcttgta tgtatacagt
     2881 catgcacacg acgccaaggc cgcgatatgc gccggggctt tgcgggtcga gatgtatggc
     2941 gtactggaag gctttgatgg ctttctggta gtattttatg cgatagcaga agacgcctat
     3001 ctgctgccac gtggcgggat tgttttcgtc gattttgcgg gcggcttcat aggtgctgcg
     3061 tgcggcgcgg tatttgcccg tgcgggtgta aaggctggcc aggttgagaa ggatgttcac
     3121 ctggttcggg tcgtactcga tggaagtctt gtagtagctg atagcctttt ccgcgtcgcc
     3181 ctgcatttcg gcgattatgc ccaggttatt gatagccttt acgttatcgc tgtcgagctt
     3241 gttagccttc gccgtttctt cgttagcctg ttcgaattgg ccgagctgga aataacacgc
     3301 gccaagatta aggtgtgcgt caaagctgtc gggcttgatg accgtggcgc ggatgtatgt
     3361 gtgtacagcc ttgttcaggt aaattctggc ctgctttacg ccttcagcca tggtggccag
     3421 tatctgatac gtcacgccga ggttatagtg cggccgaaaa gcgtattggt cggcgatgca
     3481 ggcggttttg tagctgttgc aggctttgat gtattcgcct ttgtgacggt agatgtcacc
     3541 gatggtggtg taggcttcgg agtttgaggg gttgacctgt attgctcttt cgagttcgtc
     3601 cagtgcgttt tcgagttcac cgttgcggat gcattgtctg ctgttttcga cgtatgcgtt
     3661 ggaggcggct tctttggtga ccgagggctg ttcttttttc agttggaact gtttccacca
     3721 gtccatgttc atttcacagc cgccgacaac gaaaattgcc gtcacagccc agaacgtcaa
     3781 caacttttta ccgacagact tacgcatcca cattttcctc ttgctacggt atattaacga
     3841 caataagttt tatatattac tcaaaaaagc tccggtggca ataaagtttt ttgctatttg
     3901 gcgttaagtt attattcttt atgtcgatgg aaaatggacg attacaattt cgccgccgcc
     3961 ttcggctgtc agccaagcgt gatatagacg cggtttttga ccgcggcagc cgagccggca
     4021 acgccgttct gacggtcata gcattgcaaa atccggcgaa tcgggacgtt tcgcgactct
     4081 gtgccgccgt cagccgccgg aacggcgggg cggtaatgcg aaatcgactc aagcggctga
     4141 ttcgcgaggc atggcggctg aaccggcagg attttccggc aggttacgac attgtcgtac
     4201 ttccgcggcc gggagtgaag gacgagcttg agctttttgc cgccgcactc aggacgctcg
     4261 tgccgaaggc tgccgccagg gcagggagac aacagggttg agcggcgaaa agacaaatcc
     4321 ggcagggcgg gcgctgattt tcgtcgttcg gatgtaccag gccacgcttt cgccgtttct
     4381 cggcagacag tgcagatttc agcctacgtg cagcaattat tttatcgagg ctgtccagac
     4441 ccacggctca atccgtggca gcctgctggg cttgtggcga atcctgcgat gtaatcctct
     4501 atccaaaggc ggttacgacc ctgtacctgt aaaaaaaaca aaaaacacga aataaatttt
     4561 taaaaactct ctgaaatcac aataaaaaag gcgtcactcc tgatttgtac aggactgacg
     4621 cctttcatat gttttctaaa attgctctaa gcaatcaaaa atggtaaatc gcattcttca
     4681 ttatcccatg aattcattgc cgactttgcg ggcacgctgg ggcttgtcca ggttgatgcc
     4741 caacgcaagg ccgatttcca ccaaaatatt ccagccggct gcgagaaaca cgtgcggcgt
     4801 gccttcgccg gattcaggaa ctactatcgt cgggaaaccg gctatcggcg aggtcgctat
     4861 gcagacgacc ttcaggccta cgccgtcgac cagcacttcc ctgaatttgt ccatttcctc
     4921 ggcaatgggg tctacgacga acacgatatc ggcggggctc atgacttcct caataccgtg
     4981 gcaggcgtaa gtgccttcga ggaaatcgct ctttttgcgg gttatttcgt tggttttgag
     5041 ggtgagttct tcagctacgc cgtcgttgta gccggcgaag taaatcgtag gggccttggc
     5101 ggctgcggcg acgatatccg ccggtatttc aatcgtcagc gcctgttcta tcgcatcggc
     5161 gagcctgttt ttgcaatcgc aggtgcaggc gttgctgccg gtcatgtggc cgagcatgga
     5221 ctgatacgtt aacgcctgtt cgatgacgct cttggttgcg gcgacggcgg tttcccagcc
     5281 gcagttcaga acgatggttt ccctggcata ttccttcaaa aggttttccg gctgtgccgt
     5341 caggccgaac agttccttat tgccgccgtc gcggagttgc ttgaggagtt tgacgacttc
     5401 cttcgtgcgg ccggaattgc tggcggcgaa tacggcaaat ctgctcaggt catacgtcat
     5461 tgcctggcgt gaaccttcgg taacgacctg caaattcagg ccggcggtca agaccttgcg
     5521 tataaaattc ttggcgggga aaatccggct tgaaccttcg ccggtaagca tgagtcggcc
     5581 attggccttg atagccgcgg cgacggctgc tgtgcgggaa gggtcgaatt tacggactac
     5641 atcaacagtg tcgagcattt cgcgacaaag cgcatatttc gaatattttt catttttcgc
     5701 gttcataacg acgtttcctt aaaacggatt ttttaaaaaa aatttcaata ctgacttgat
     5761 aacggcagat gcggtgatgg gtacgcatca ccagccgtcc tgcagcgtta ccggcctgca
     5821 ttgaggttct tttgcgtcaa gttgtgaacc ggttattatc ataaaaaatt cattttgccg
     5881 gaatgtactt caattcggcg tattcgtcaa gctctcctac gagcggacgg atgtaatcga
     5941 aaaatgcctg cgacgggaaa ttgccttctg cgttgatgaa gctgtccggc atgggcttgg
     6001 ccttgacggc tacgtctttc aacgggactg tctcataact gacggcatac ggcgagtcgc
     6061 ttttgcggac gatggaaacc atgacgccgg tcttgccgga gtcagccagt tctacggctt
     6121 tgcagccaca ggcgtaggct tcatccaaat cctgcccaac ggctcggtct attgcgctca
     6181 tcggcaggga ttccgttatc tggaactcgc cgcgccagcc gaactcgtcc ttaatcatgc
     6241 gatgcaggtt caacgccacg ctgctgccgc ccattgcgcc gaattcctca ttgccgaact
     6301 tgtcttttgt ttttgtcgcc gacaccggcg tgccgtcggc atattttatg ccttcgccgc
     6361 aaactatcga cacgaatccg tatttttcgt gcgtcgattt gacgtcggct aaaaattttg
     6421 ccttgtcgaa gacgcgttct ggaatataga tgagatgcgg cgcgtcagcc ggatttttcc
     6481 tggtaagcgc tgcagcgcag ctcagccagc cggcgtctct gccgatggac tggtagatta
     6541 cgaactggtc gactttctgc atgtcgcgag ccagtcggcc agcctgctgt acgctcagtg
     6601 cggtgtaacg tgccgccgac gcataaccag gtgtgtggtc tgtcccgaag aggtcgttgt
     6661 ccaccgtctt gggtattcct atgccgcaca tctcgtaacc gtgggagttg gcgtattcag
     6721 tgacgcgatg gatggtatcc atggtgtcgt tgccgccggc catgaaaatg gccctgatgt
     6781 cgaattcctt cagccgttgc agaatgaccg gaaaatcagg gtctttgacc ttgtgtcgac
     6841 ttgaccccag cgccgacgaa ggagtacggc gaagcaggtt taccgtttcc tgcggctcag
     6901 ccgacaggtc tacgacattg ccggccatga agccctcgat ggcccatctc atgccgagaa
     6961 ttttcccgac attagccgct tttcgtccgg cgtcgattac gccggccagc gatgagttta
     7021 tcacgctggt cgggccgccg gactgcccga taatgatgtt tcctttcaac atatcgtacc
     7081 tttccgttga aatgaacacg aaagcatttt ttcaagtttt tcaattcggc tgcactttcg
     7141 cggcagtcat gcttctgcgg ctgcacaaga aatgcgtgct ctctttttta aaaatgcctt
     7201 ctattgacaa cttcgctcac gacgcataac atctgcgcca tgaatacgaa gcagaacagc
     7261 aaacggatat tatatgcagg cgacacgtcg ttgacgacgg cggctgcata cctggccggc
     7321 atcatgacgc attacggccg ggatttcgac tacctcgcca gcaatcagcc tctcgccgac
     7381 gccccgcacc agcacggcta tgccttatac ataatcagcg actatcctgt caataactgg
     7441 actgataaag agttcaaaaa tgtcgcagac gacgtcgaac agggggctgg ccttataatg
     7501 cttggcggct gggaaagtta ttgcggcctc ggcggaaact atcagaatac gccgatagca
     7561 gacatcctgc cggtgaaaat ttcgccgttc gacgaccgcg taaattcacc tgtgccgtgc
     7621 cttatcgaag tatgcagaaa acatcctata atcgacgctc tgccgtttga caggccgccg
     7681 gctatcggcg gttacaaccg cgttacgctc aaaccaggct cgcaggagat actttctctt
     7741 cggccgatag acataatagc aaccggtggc ggatatgatt ttgcggtcgg aacggctgag
     7801 ccgctgctcg ttacaggctg ctggggcgac ggcagggtgg cttgcctgac gacggacgtc
     7861 gcaccgcatt gggtgggcac acttgtagac tggggcatga agcgaattac ggcaaaagcg
     7921 ccgcatgcag acagcatcga agtaggagaa aattacgcaa aattctttaa ccgtattatc
     7981 ggctggactt tgggcgaaaa attgtagttc ggtttgtcgt taattttacc tttgacgcat
     8041 tggcgttttt taggaattgc taacagataa tttgtctgcc gcgaatccaa aacagcgaag
     8101 aaaaacatac ttttgcgatt atttcaacgg tacagccgag ttatccccaa taaaaaacgc
     8161 agcttgtgga taagtcgtgg agagaagttt ttttttacgg ttaagttcgt tttgactatg
     8221 cggttatcat gtgtggataa gttttgtaat tctttagtgt tgacccgtat gaacctcgga
     8281 cgtaaagtgt gtcagtcgtt gctgctcatg agcagcggcg attggctgaa agcctgtgat
     8341 atttatgcag ttcgtcacgg gaagaacggc ttgcaacgat aaaagtgaga cagttttcat
     8401 aaaatacaaa gggaataagt atgaccatga ttgcggaaga tctctgcgca gagcaaatgc
     8461 taaaaccggt tatgactacg ctgcaggatc gtatagggcc gcaaaaattc aacgcatggt
     8521 tcaagcacgg cacggaagta gcgttcggcg acgaccacat aaaggtactc gttcccaatc
     8581 cgttcgttgc aaactggata gagcatcatt accagagcga ccttgtggag tcgtttttcc
     8641 agcatactcg ccgccggctg ccggnnnnnn nnnngcagca aacgcgactt cgatatgcag
     8701 gctgaaattg tcaagcgtac cacgcagggc cgcgtccggc cgagaaaact tcccgccaac
     8761 caacagctca aataccgcct gagggatttc gtcgtcgggc cgagcaacta tctggcatac
     8821 caggccgccc tgaatgccgc caatcccgac aatcgcaatt ttacgcaact tttcgtacat
     8881 gggccgtgcg gcgtgggcaa gacgcacctg ttgcagggca tatgcaatga attagcgagc
     8941 gcacagacgg aaaacggcag gccgttgcga tggcgatacg tcacaggcga acagtttaca
     9001 aatgaattca tcgccgccgt ccgaacgcag aaggcagggg agttccgcga aaaatatcgc
     9061 agccttgatt tgctggcgat agacgatgtg catttcctgg ctgccaagcg ggcaactcaa
     9121 gatgaatttc tgcacacgtt caacgccatt gaatccgccg gccgccagat tattctcgcg
     9181 tcggattctc atccgcggct ggtcggcgac ctgaacgaag gcctcgtcag ccgtttttta
     9241 gccggcatgg tcgtaaaggt tgactcgccg gacaaagata cgcggctgga gattcttcgc
     9301 cgcaaagcag ccgggttgaa acttgcactt tcgccggaag tcgccgatta tatcgccgta
     9361 catatccgcg gctcggtgag ggaactggaa ggcacggcgg tgaaactttc ggctatcgca
     9421 gccttgcagg aagaaccgat aaacctgacg ctggtacggg aagcgctgag cgaccacctg
     9481 gcacgtacag acagcgcaac cacgctcggc gatatagaag ccgtcgtcgc tccgtttttc
     9541 ggcgtaacgc cggctgatat ccattcgtca cgccgtacaa agaccgtttc ggcggctcgc
     9601 atggtggcta tgttattggc ccgccgctat acgccgatga gttttccgga aatcggccgg
     9661 ttcatgggca aaaatcacag ctccgtagtg ctggctgtcc agcgaatcga gcagtcgata
     9721 aataaagatg aaaatatcga ctggaatacg ccggctggcg taaaatccat gcctgcgaaa
     9781 acaatcctgc aacttttgga agaacagctt aattattaga atttcaaatt gcggaatttc
     9841 aaattgcaga ttgaagggga aataaaagta tgtacgaact tttggagttt ttaagtttcc
     9901 ggcaccagat ttaatttcat aataaaacat ccacacccat tgcgtccgat aatatatatt
     9961 atgttgcgtt gcaagtcttt tatttgcaaa tatttacatt tttacttcct tgcaaattac
    10021 ttttttactc ttgttacttt atcagttaaa agtttcaata aacaagatga caacatacac
    10081 gtaatttatt taaaaaattc aaactgaccg aaccatttga cacatatatc gtaactgctt
    10141 gcaaaataag tctttatcga accttataac gcgaatcggt cactttgagt aaaaaattta
    10201 aaaacttcaa aaaaaacaag ttttaaattt cccgaataat cattattcga tattttgggg
    10261 tgcttgaaat ttggctcttg aatcctgctt acttgcttgt aagttgtttt ttgattcttg
    10321 ttacttttac ctggaagtat catggctgag ttacagataa aacttgaagg cgtatgtttg
    10381 tcgcgtggcg gcgtggagat tttgcatgat attgcatggg aagttttagc cggccagcga
    10441 tgggttttgc ttgggccgaa cggttccggc aaaacgtcaa tgctggaagt catcacgggc
    10501 tacgaatggc cgaacgtcgg ccgtgttacg ctgctggacg aacggctggg tgaggttgac
    10561 attcatactc accgtcgtcg gataggtttg gccagcaccg gcatagacca tttcatcaaa
    10621 aaacatcaga ctgcgttgca gattgtcttg agcggatttt ttgattccac cggccttttc
    10681 gattcgccga ctacgcggca gattcataca gcccaaacaa tgcttgatat gctcggctgc
    10741 ggctacctgg ccagccgccg attttcatta ttgtcactcg gcgaacagcg aaaagttctg
    10801 attgcccgca gcctggttta tagccccgaa atgcttattc tggatgaacc gtgtgccggc
    10861 ctggatatag ctgcccgcga atttgtctta aaaaccatcg ccgaactcgc cggcaacggc
    10921 cattcggcgg cgctgagctc gattatatac gttacgcatc atatcgacga aattctaccg
    10981 tgttttacgc atatattggc gataaaatcg ggtggaatcg tggcggctgg gccaatcagg
    11041 cagactctta caagtgattt aatgacagaa ctttacggca ttcctattga ggttggatat
    11101 ttcggcggac gctacactgc aagagtccga taacttaatt atgaggcctg ttgcagggcg
    11161 gctgagccgg aatttccaat taaaaaacca tccggaatta tccggacggt tttttaattt
    11221 tttaaataat tttctacaaa aaaaaatgga gctgaggggg ttcgaacccc tgaccttctg
    11281 catgccatgc agacgcgctc ccaactgcgc cacagcccca gtcaggacga atcttttacc
    11341 agttaacatc gctggcgtca agcatatctg gggctttttg caaaattatt tctgcggctg
    11401 gaagtctcaa acctgaaatg cattaaggtt ttttgacaag aaggaaaaga ctttcgagaa
    11461 ttgttctaaa agctggatgc gatttcgcaa ggcaggctga attcttcatt taccagccgc
    11521 gcgaacactt cggcggcatc gcgcgtctca aagatagaaa aaagagtgct gccgctgccg
    11581 gacatgtgga tgagtttgtc tgttgtgact ttcaggcggc tttgcagcag tcccagctcc
    11641 ggtgccagcc taaacgcccc ctcttcaaga taattcacca gcaacgattc ccataacccg
    11701 cacgggctct gccatatttc atcatccagc tggccttcca gagtttgtgg attctggtcg
    11761 taggagcaat aaacttcgcc cgtgggacat ggaaaacccg gaaacatgag aaccgcgtga
    11821 aaactgctga cagccagcgg cgtgatgtta tctcccctgc cggtcattct gcaacagccg
    11881 tcgtacatga aaaacggcac atcgctgccc agccgccccc ctgccgcggc taacgattcg
    11941 cgggacatcg gtgaaccgaa catgtcgttc atcgccatca ataccgtcgc acagtcggac
    12001 gagccgcccc ccaatccgcc gccggtagga atttttttgt gtatcgcaat atccacgccc
    12061 ggacagtcat tgatttcgcg aaacagcgcc gccgccctgt atgccaggtt tttttcgagc
    12121 ggcccgcaat ctatagcccc gctgacgctg cacgtgattt cgccgtcgtt acgcggcgtc
    12181 acgcagacgc ggtcaaacag ggatactttg gctgcaaccg agtctatggg atgaaaaccg
    12241 tcatcgccga cagggccgac cagcagattg agatttatct tcgccggcgc attgtgctcc
    12301 aacgcactct gcgaagcttc gtttgcggtt cggttgtcag tcatttatcg cgtccatcct
    12361 tgctgaaaaa tcggcacgta tcgcgagact cttccggcgt gaatatcggc acgccgtctt
    12421 cgacgttgtg aatgtacttt tcgagattag cctgcttttc ccgtatcagc gagttttcct
    12481 gcatgatgcg gggcattctg aaataactcg gacgataaca cctcatgtca ctatctccca
    12541 cgtaagcgta tccaccgttt acgtcggctg gcactcctta tccacgcatt ttgtcgggat
    12601 agctaacggt aagcagggca atcgacaagc tgcatgttaa ggaatactga actgctgcgg
    12661 caaatatcac gaataatctg tttattccgt tatatgtttg ccggccgtcc atgaggggca
    12721 taaaacgccg cttatccgtc gcgacgaggg tttttccgga tttggccgtc aggctgctta
    12781 tgcggcgagg cggccactac gttcatccgg cattcgcagg ctagttcgaa aaattctttt
    12841 ttttccaaat cctccagctg tttcccgcga acgtccagtt tttcgttgaa ttttatgcac
    12901 ttttcctgca tctcttcgtg agtttcacgc gagccgccca taagatgaaa attcttgcct
    12961 tttgtaatcc tgatatggcc atcctgatta tcaaggccaa gccctatgag catggcacga
    13021 tttttatcat catccgacaa agtacatctc cagatattca acaactgaaa aatgaaaacc
    13081 taccagattg attataaaaa aaatgcagcc tgattcaaag ttttatttga atgatggcgg
    13141 ccggcaaaaa aaaagcattt gacttgactg taacggcggg ttgaatatga tttattggct
    13201 ttgcagaaag gaatgcgatt gatggaatca aacaatataa cggtagtagg gttacaatgg
    13261 ggcgacgaag gaaaaggcaa gatagtcgac gcccttgcga ggacgagttg ttatgtaacc
    13321 cgttactgcg gcggagctaa tgcgggccat accgtaaaag ccggcggcga gaagtttgcc
    13381 ctccatctca ttccgtgcgg catactccac gagaaagtga ttaacgtcat aggcaacggc
    13441 gtggcgttcg atccggaagt cgcatggggt gaaatcgtcg cccttcgcga acggcacata
    13501 aggatagatt cagaaaattt ccgcatatcg tcgtgtgccc agcttgtaat gccgtggcac
    13561 aagtatcagg acaagttaaa cgaacagtcg ctgggccgaa ataaaatcgg cacgacggcc
    13621 agaggcatcg gcccgtgtta cgccgacaag gctaaccgtt cgacctccat ccgtgcatgc
    13681 gatttactgg acgtcgcgtc gcttgagcag aagatacggc acatcggcca gcagaagaac
    13741 accgtttttg ccgcacttta taacgccgct ccgatggaca tcggcactat cgtcgcccag
    13801 ttccgcagcc ttgcggaaaa tctttcgccg atgatatgca acacgtccgc cctgctccgc
    13861 aacgcatgtc aagccggccg tcgcatactt ttcgaaggcg gccagggcag catgctcgat
    13921 atcgaccacg gcacttatcc gtacgtgacg agttcgccgg ttaccgcctg cggcgtaccg
    13981 gcaggcgcag gcgtttcgcc caaaagcgtc ggccgtgtgc tggggataat gaaagcatac
    14041 accacgcggg tcggcgcagg gccgttcccg acggagctgg acaacgaaat cggccaggca
    14101 attcgcgacg ccggccatga atacggcaca actaccggcc ggccaagacg atgcggctgg
    14161 ctggatgcga tggtcgtccg ctataccgcc agcctttcag gcgtggacga acttgccctg
    14221 atgctgcttg acgtattgac gggcttcgac gaattgaaga tatgcaccgg ttataaaatc
    14281 gacggtgaaa ttgtgaaaga cttcgaccct gcacggctcg acgaggttga atgcgtctac
    14341 gaaaccatgc ctggctggca gcaggatata actgattgtc gcaaattttc cgaactgccg
    14401 gcggaggcgc agaattacgt tcgccgtata agtgagatag ttggcgtacc cgtcggcata
    14461 atcagcgtcg gccctgaacg tacgcagacg atattgcatg aatcggaaat taaggaactc
    14521 gcatgatgtc agggcagcag cttgaaaagc gtttttatga agcgaggcag gaaataggct
    14581 gtgacccgat gaaatttccg gagtcgatag ccataataat ggacggcaat ggccgatggg
    14641 ctcagatgcg cggcctgccg cggccgttcg gccataaaga aggcgccaaa gtagtccggc
    14701 agatagtcaa ggaagcggct cgcctgggcc tcaaggcact gaccctttac agcttttcac
    14761 atcagaactg gtcacggccg gcggaagaag tcgacgccct catgcacctg ttttccgaat
    14821 atcttgcaca tgaacgcgag actgttatgg aaaacaacat ccggctgcgg catctcggct
    14881 cacggcaggg cctgccggat atggtgctgc acgaactgga tgaaaccgtc agacagtcgt
    14941 cgaaaaatac gggcatgttt ctctgcctgg cgttgaatta cggctcg
//
LOCUS       DAONFI010000009        14904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_4346_length_14904_cov_4.336858, whole genome shotgun sequence.
ACCESSION   DAONFI010000009 DAONFI010000000
VERSION     DAONFI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_14904_cov_4.336858"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLK08_00675"
     CDS             <1..356
                     /locus_tag="PLK08_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52041.1"
                     /translation="AAVRYEPIASAANLVRTAGELPKFPPIRRDLSITVKENVTWRQI
                     QEVLAAKPQPLRVADEYVTTYRGRQIPKGCKSVTMAFEFRSNDGTLQHEQVDVLMDDI
                     VAAMKTAFNADLRTA"
     gene            353..1642
                     /gene="hflX"
                     /locus_tag="PLK08_00680"
     CDS             353..1642
                     /gene="hflX"
                     /locus_tag="PLK08_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPS52042.1"
                     /translation="MMAELRETFEIESERTLLVCVVLPGDNHDTHDPLGELRALAKTA
                     GSDVVDEILCKRQQPHTGLYVGTGKASQIADRCEMNEIDVVIFDNDLTPGQIRELEAV
                     TKTKVIDRSELILDIFAMHAGTHQAKLQVELAQLEYTYPRLTGMWSHLDRHGGGVGTR
                     GPGERQIETDRRIVQKRVGMLKEKLDAIDRRKLQEVKNRNREFCACLVGYTNAGKSTL
                     MNLLTDAQTYAEDKLFATLETKTRKWELSADCPALLSDTVGFIRNLPHHLVASFRATL
                     EEAIFADLLLHVADASSHRVFEQIDAVHKVLAELGCDPERELLILNKTDRIIDPTVYA
                     VLRQRYPKAVTMSAMSGNGAEKLIAAVRDRATGLPTPVKLRANFRNGRLMSFLARHAK
                     IENQTYLADFAEIDAVIASDRLRELLNAFRNDIAVMD"
     gene            complement(1668..1898)
                     /locus_tag="PLK08_00685"
     CDS             complement(1668..1898)
                     /locus_tag="PLK08_00685"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HPS52043.1"
                     /translation="MILKIKKLLAKRKRMTLGEIAIHFDADPQAIEPMMQKLVDGGIV
                     RKYKPAAARLCGSCKICGEQCDLTMIVYEYLN"
     gene            complement(1895..2188)
                     /locus_tag="PLK08_00690"
     CDS             complement(1895..2188)
                     /locus_tag="PLK08_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HPS52044.1"
                     /translation="MMFERFRRRFRAGRRERCCCTDTSAECIPLALGQVGNKYVIFRN
                     SDAKTREMGLYPGAEVHVIKNTRGDTGIVVAVGEARYAVHRDIAGLIIVRPGT"
     gene            complement(2191..4464)
                     /gene="feoB"
                     /locus_tag="PLK08_00695"
     CDS             complement(2191..4464)
                     /gene="feoB"
                     /locus_tag="PLK08_00695"
                     /inference="COORDINATES: protein motif:HMM:NF007105.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HPS52045.1"
                     /translation="MYDTTNDRTSYTIALAGNPNAGKTSVFNALTGMKQSVGNWPGVT
                     VERLEGKYFYDGKKVTVVDLPGIYSLNAYSVDEAVSRKYILTEKPSVVVNVIDATNLE
                     RNLYLTSQLLEMRVPMVAVLNMTDIASQRRIRIDVEHLRSHLDCPVVPMVASRKQGVD
                     ELKSAIAEVAASGHVPSTFPIYDEEVEEAIDAVMSYCQALSLDKAVSPRWLAVKMFER
                     DRWAMLFASGELADKLDKHISHIERHTGEKIDLVIADGRYGFVHGLVSDVLRRRRRLR
                     RTVSDAIDRVVLNRAVGIPLFLLIMYCMFFLTINLGDPFINFFDRFCGTLFVDGPNYL
                     LAKAAAPRWLGILLADGVGGGIQTISTLIPPIFFIFLSLSILEDSGIMARAAFIADRL
                     LHRIGLPGKAFIPLMVGFGCTVPGILATRTLESRRDRLLTIMINPFMSCGARLPVYVL
                     FTVAFFPVSGVWVVFLIYVIGIILAVCTGVLLKNTILKGTAVASVMELPPYHIPTLGG
                     VMHHTWNRLKGFVFKAGKSILLVVVALNCLTEFTVGDENIISKIGRTISPVFEPMGIR
                     RDNWPASVGLCTGIFAKESVVGTLNSLYASQKQSDAGGESAAKFDFVAQMADAFSAIT
                     DGFKKLGVKLIDYSGASDELGNDDENDAFAALRKNFRTSSAAFAYMLFVLIYAPCVAA
                     IATIFSETNWKWTAFSVGYLTLLAWLVATAFYQASILPAQPAISAVWLTTVAGIVVVL
                     FAVLRAVGRRMSVEYKE"
     gene            complement(4515..5789)
                     /locus_tag="PLK08_00700"
     CDS             complement(4515..5789)
                     /locus_tag="PLK08_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HPS52046.1"
                     /translation="MYKFLAVWMVVGFASTTSVAQTESQSSSAINPFKILPADTLTDN
                     WFGLGRKLAQSGITIDLNVTQLYQQNLGGGLSTHRRAGRYSGRYDLELNADMGRLFNL
                     PGGRVYMLTRGGWSDGIDASSVGSIFGVDGLEFGDEPADVWELYYEQKMFNDRMILRV
                     GRVDLTCGFECHDAPASFDCNTYANDEVTQFLNNSLVNNPTIPFPDPGFGVILHITPV
                     DWWYVTAAVVDADADYRETGFNTAFHGPSNTFSIYETGIMPDFYSPKGRLPGAYRVGL
                     WYDPQAKERFDGNGVKRDDAGFYIDCDQMVFKENAVEDDSQGLGVFARYGYADGDVNE
                     IKNFWSAGVQYQGLIDGRDDDVLGVGVGQGLLSRKADFTASNETALECYYNMQLTPWL
                     HVSPDIQYIFNPGGDSDVSDAVVLGFRVQVTF"
     gene            complement(5820..6785)
                     /locus_tag="PLK08_00705"
     CDS             complement(5820..6785)
                     /locus_tag="PLK08_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52047.1"
                     /translation="MSTCTTHIKKSIVLCLLLAVGLMSGRICMAECTDCDGCEGGSCT
                     KKVSEQCSECVLKNQKIAKEKFLAGLELIRKDKWNDALKNYRGSASVETKFKRVYAKQ
                     AELLQAAVMHRALLEKPHLSADDIKAIKNCWRYCITRNAMTPAMAIAEKLNTVVKTHE
                     TKELLAETYMLSSENQKAYDVLTAMTFDSMTQGARMTYGIVSARLGMKDEASKIMDGL
                     DMSKIPANLRYKYAMLLAAAGKSSEAIEMLKMILEGQCGLGAKRMKFQIEKTPDFESL
                     KSSAEYKKLFTTGENDCNSCPNRDKCSQSSRRLGYAEFSIVFKGG"
     gene            complement(6812..7459)
                     /locus_tag="PLK08_00710"
     CDS             complement(6812..7459)
                     /locus_tag="PLK08_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52048.1"
                     /translation="MLLPSLSRAKALARRVVCLANLSGIGKMVYLYACDNDEYYPDPY
                     AMGAGGFTYSDGEKIYYGQSYFRCGVGFSYKEGLPEQYGLMGALLNAGLATAPSAWIC
                     PGATEEMALFGNTYWNSGNSVLGNKRLGDYTSDELFDLGLLSDNHWFLPANSGVRGAV
                     QRCLLATKRVFPHAEVGIKDWTNINGWGYELTKKSPRNNNILFYDLHACVDYERH"
     gene            complement(7626..8690)
                     /locus_tag="PLK08_00715"
     CDS             complement(7626..8690)
                     /locus_tag="PLK08_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS52049.1"
                     /translation="MNALRHLGLIAAIAITSCGSMAAVYKQILSLDDMRSTEGSGNVY
                     TVIIEDDDAYCILSNSSGGQSGRITKISGVSTSKTYSQILSTAEWNEANGGVTSSLAT
                     IYDGRIVGDYIQFSDSSTDAIWRVNRNTGEVTCYASQADILAATGQTAVSLASPFVVY
                     GGEHYVYEGTSDSIIRTNGANNVDIFLTSAQLTAISGNSTVSGGIDFDSSGNLYWGSN
                     DSDSLYSWDGANGSTVLDVADITAVTGGSSAGFGDIYYGQDGNVYFYETTTKSILCFD
                     VNATSAADSLTMLFSAAELIAGDAASNSVGTFAWYDGHIAWSTISTLANIGFYVAVPE
                     PATIVLLTIGTFGAMFRRRR"
     gene            complement(8861..9058)
                     /locus_tag="PLK08_00720"
     CDS             complement(8861..9058)
                     /locus_tag="PLK08_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52050.1"
                     /translation="MKANSCFCESPVLAKISELYRELIAHDGFGELHMDVRILKRGQK
                     EVIIHCGKQYRYVVDFPQKQS"
     gene            complement(9213..9614)
                     /locus_tag="PLK08_00725"
     CDS             complement(9213..9614)
                     /locus_tag="PLK08_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPS52051.1"
                     /translation="MAKTKHINISDSLEDYLEAIFFLIQQKQAARAKDIGRQLGVSRP
                     SVTGALHALADRNLVNYSPYGIITLTQQGQAIAREVYRRHEILRDFFVKILDVEYSEA
                     DSAACKMEHVISDAVLKKFLNFIEKCSRPDL"
     gene            complement(9849..10262)
                     /locus_tag="PLK08_00730"
     CDS             complement(9849..10262)
                     /locus_tag="PLK08_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52052.1"
                     /translation="MKKAIVAVFLGGLIFAGCDKPVEIEFQKPTGTKMVTMGHEYTWP
                     AKITFQRSDDVNQIKDYDVKLTIPSNQGMIEASGKISFYPFKATDEATLTTYMFSFSA
                     KDLERLYNGEVITVKADNADDQLMYKMILGTGKNK"
     gene            10879..12279
                     /locus_tag="PLK08_00735"
     CDS             10879..12279
                     /locus_tag="PLK08_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPS52053.1"
                     /translation="MLTLFWKNATHKRVGARNGVTPTDLAEIEPRLRKAHEAVVSQCH
                     DGKLGYATLPTNETYRKQVTDLVARYKENTTDMVVLGIGGSALGNIALQSALNPSTYN
                     ILTERKRKGPRLFVLDNVDPCYVHDVLKFVSRRASSTLVNVISKSGETAETASQFMLF
                     RQMLRDKIGDNFASRIVATTDASKGTLHDIAKKEGYAMLPVPGDVGGRFSVLSPVGLF
                     SAAMCGIDIDKLLEGANAMRQRAEVMDVMQNPATILAAIKYIMFTKKKKPMHVMMSYS
                     NRLYMLADWYRQLWAESLGKQYDREGNEVFTGPTPIKALGTTDQHSQVQLYREGPNDK
                     FTVFLDVKKQPEDIRVPNTFDDIQGMSYLRKKRMSKLLQAEKLATEYAMTVSQRPSVT
                     LTFPEISERYIGEFLFLYEFTTSIMGELMNINAYDQPAVELGKKATFALMGRKGYEEL
                     AEKMKSFVRMDRRYQA"
     gene            complement(12504..13253)
                     /locus_tag="PLK08_00740"
     CDS             complement(12504..13253)
                     /locus_tag="PLK08_00740"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HPS52054.1"
                     /translation="MQQTITPEICAAKIRNAKNIAVLTGAGISTAAGIPDFRSPTGIY
                     NSGRYNADVVFDINYFYRDPSEFYRFTRDLLAIVDTLSPTFTHRFLADMERAGMLSAV
                     VTQNIDPLHYTAGSKNVIPIHGNYNTSHCLECGREYSLADLERMLGEMDIPRCDCNKR
                     GIIKPDVVFFGEAVNRIFDAQRAARESDLFFVLGSSLVVYPAAMLPHIAGGEVVIVNR
                     GPVAFEPADGRYFVDGDLDGFFRKVAEFIKL"
     gene            complement(13572..14171)
                     /locus_tag="PLK08_00745"
     CDS             complement(13572..14171)
                     /locus_tag="PLK08_00745"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPS52055.1"
                     /translation="MLLTVRETILGIVMIDGRTVKVLLSLLVAMTGGALLLMSMDSMP
                     PQNATSASIIGKVDYTQMGLTHTWRRIVVHSATDMNDSLPQRCHFIISADANTSGKGV
                     VATDLWTRQVRGYHVMIPGHDFDSDSIGICLIGDFSAKGPGREQFNALVTLVNKLQKR
                     CGIAADCVYINNQLDPSSDQPGKAFPTEMFNRYLLKNLD"
     gene            14637..>14904
                     /locus_tag="PLK08_00750"
     CDS             14637..>14904
                     /locus_tag="PLK08_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPS52056.1"
                     /translation="MKLPVYLDNNATTPVDPRVLDAMLPYLKDHFGNAASRSHSFGLE
                     ASHAVENARQNIADLLHTETKEEIVFTSGATESDNLAIKGIAEKL"
BASE COUNT         3904 a         4038 c         3402 g         3560 t
ORIGIN      
        1 tagccgccgt acgatacgaa ccaatagcat cggcggcaaa cctggtgcgg acagccggcg
       61 aattgcccaa gttcccgcca atccgccgcg acctttcgat aaccgtaaaa gaaaacgtca
      121 catggcggca gatacaggaa gtcctcgccg ccaagcccca gccgctgcgg gtagccgacg
      181 aatacgtcac aacataccgc ggcagacaga taccaaaagg ctgcaaatcg gtcacgatgg
      241 ccttcgaatt ccgctcaaac gacggcacat tgcagcatga acaggtggac gtactcatgg
      301 acgacatcgt ggcggcgatg aaaaccgcgt tcaacgccga cctgcggact gcatgatggc
      361 tgaactgcgg gaaacattcg aaatcgagtc cgaacggacg ctgcttgtct gcgtggtgct
      421 gccaggcgac aaccatgata cgcacgaccc gctgggtgaa ctgcgggcgt tggctaaaac
      481 agccggctcg gacgtggtgg atgaaatcct ctgcaaacgg cagcagccgc atactggcct
      541 ttacgtcggc acgggcaaag cctcgcagat tgcagaccgc tgtgaaatga acgaaatcga
      601 cgtggtcata ttcgacaacg acctcacgcc cggccagata cgggaactcg aagccgtcac
      661 caaaaccaag gtcatagacc gcagcgaact gatacttgat atcttcgcga tgcacgccgg
      721 cactcatcag gccaagttgc aggttgaact cgctcagctt gaatacacgt atcccaggct
      781 gacgggcatg tggtcgcatc ttgacaggca cggcggcggc gtgggcacga gagggccggg
      841 cgaacggcag atagagaccg acaggcgaat cgtacagaaa cgcgtcggca tgctaaaaga
      901 aaaactcgac gcgatagacc gtcgcaaact ccaggaagtg aaaaaccgca acagggaatt
      961 ctgcgcctgc cttgtcggtt ataccaacgc cggcaaaagc acgctcatga atctgctcac
     1021 cgacgcccag acctacgccg aagacaaact tttcgccacg cttgaaacca agacccgaaa
     1081 atgggaactg agcgcggact gcccggcact gcttagcgac accgtgggat tcatacgcaa
     1141 tctgccgcat catctcgtcg ccagtttccg ggctacgctc gaagaggcta tttttgccga
     1201 cctgctgctg catgtcgcgg acgcatcgtc gcaccgcgtg ttcgagcaga tagacgccgt
     1261 tcacaaagtg ctcgccgaac tcggctgcga ccctgagcgg gaactgctta tcctcaacaa
     1321 gaccgacagg attatcgacc ccaccgtata tgccgtcctg cggcagagat atccaaaggc
     1381 tgtcaccatg tcggcgatga gcggcaacgg cgccgaaaaa ttgatagccg ccgttcgcga
     1441 ccgcgcaacc ggcttgccga cgccagtgaa attgcgtgca aatttccgta acgggcggct
     1501 gatgagtttc ctggccaggc acgcaaaaat cgaaaatcag acatacctgg cagacttcgc
     1561 cgaaatagac gccgtgatag cctccgaccg tctaagagaa ttgctcaacg ctttccggaa
     1621 cgatatcgcc gtcatggact gaacgtcatc atccgcatgc ggcggcatca attcagatat
     1681 tcgtagacta tcatggtcag gtcgcactgt tccccgcaga ttttgcaact gccgcagagt
     1741 ctggcggcgg caggtttata tttacggaca atgccgccat ccacgagttt ctgcatcatc
     1801 ggctctatcg cttgagggtc tgcgtcaaag tgaatcgcaa tttcgcccag agtcatgcgt
     1861 tttctttttg cgagcagctt ttttattttg agtatcatgt tcccggccga actattatga
     1921 gtccggcaat gtcacggtgt acggcataac gcgcttcgcc gacggcgact acgatgccgg
     1981 tatcgccgcg agtatttttg attacatgca cttccgcacc ggggtaaagg cccatctccc
     2041 tggttttggc gtcggaattc cgaaatatga cgtatttgtt tcccacctgg ccaagcgcca
     2101 gcggaatgca ttctgccgac gtgtctgtac aacagcacct ttcacgtctg ccggcacgaa
     2161 atcttcttct gaatctttca aacatcatgg tcactcttta tattcaacac tcatccgtct
     2221 gccgacggct cgtaaaactg caaaaagaac cacaacaatt ccagccaccg tcgtcagcca
     2281 tacagccgaa atcgccggct gagccggcaa gatgcttgcc tggtaaaacg ccgtcgccac
     2341 cagccaagcc agcaatgtga gatatcctac tgaaaacgca gtccatttcc agttggtttc
     2401 gctaaaaatt gtcgcaatcg ccgccacgca cggcgcatat atcaggacaa acagcatata
     2461 cgcaaaagcc gccgaactgg ttctaaaatt tttacgaagc gcggcaaaag cgtcgttttc
     2521 atcatcatta ccaagttcat cgctagcgcc gctgtaatct atcagcttga cgcccaattt
     2581 tttaaagccg tccgttatcg cggaaaaagc gtcagccatt tgtgccacga aatcgaattt
     2641 tgcggctgat tcgccgccgg catccgactg tttctgactg gcgtaaaggc tgttaagcgt
     2701 gccgaccacc gattctttgg caaatatgcc ggtgcacaat cccacgctcg ccggccaatt
     2761 atcgcgacgg atacccatgg gctcaaaaac aggcgatatc gtacggccga ttttggaaat
     2821 gatattttca tcgcccaccg tgaattcggt aaggcagtta agggctacga ccacgagcag
     2881 gatagatttg ccggctttga acacaaaacc tttcagccga ttccacgtgt gatgcatcac
     2941 gccgcctaac gtcggtatgt gatacggcgg aagctccatg accgacgcca ccgcagtgcc
     3001 tttcagaatg gtgtttttca gcagtacgcc ggtacatacc gccaatataa tccctatgac
     3061 gtaaatcaga aacaccaccc atacgccgct gaccggaaaa aacgccaccg taaacaatac
     3121 gtaaaccggc agacgcgctc cgcaactcat gaacggattt atcataatgg taagcagccg
     3181 gtcacggcgg ctttcgagcg tgcgcgtcgc caatatgccc ggcacggtac agccgaatcc
     3241 caccatcagg ggtatgaacg ccttgccagg caggcctatc ctgtgaagca gcctgtcagc
     3301 gataaacgcc gcgcgagcca ttatgccgct gtcttccaga atcgacaggc tgagaaatat
     3361 gaaaaatatc ggcggaatca gcgtggagat tgtctgtatg ccgccgccga cgccgtcagc
     3421 taacagaatg cccagccatc ttggcgcggc ggctttcgcc aacagataat tcgggccatc
     3481 cacgaaaagc gtaccgcaaa atctgtcgaa aaaattgatg aacggatcgc ccagatttat
     3541 cgtcagaaag aacatgcagt acattatcag caaaaacagc ggtatgccca ccgccctatt
     3601 caacacgaca cgatctatgg catccgaaac cgtcctccgt agccgtcgcc ggcgacgcag
     3661 cacgtccgat accaacccgt gcacgaaacc atatcgcccg tcggcaatca cgagatctat
     3721 cttctcgccg gtatgccgtt cgatatgcga aatgtgctta tccagcttgt ctgcaagctc
     3781 gccggatgcg aaaagcatgg cccatctgtc gcgttcgaac atttttaccg ccagccaacg
     3841 cggcgaaacc gccttgtcca gcgaaagagc ctgacaatac gacatcacgg catcgatggc
     3901 ttcttcgact tcctcatcat aaatcggaaa cgtcgacgga acatggccgg aggcggcaac
     3961 ctctgcaatg gctgatttca gttcgtcaac gccctgcttg cgagatgcaa ccatcggcac
     4021 gacaggacaa tccagatgag acctcagatg ctcgacgtct atgcggatgc gccgctgcga
     4081 cgcgatatcc gtcatgttca acaccgccac catcggcacg cgcatttcaa gcagttgcga
     4141 cgtcagataa agattccgct ccagattggt ggcgtctatg acgttgacga cgacggaagg
     4201 tttttcagtc aaaatatatt tgcgggagac ggcttcgtcg acggagtagg cattcaacga
     4261 ataaattccc ggcaaatcga ccacggtcac ttttttgccg tcgtaaaaat atttcccctc
     4321 cagccgttcg accgtcacgc ccggccaatt gccgacactc tgcttcatgc ctgtcaacgc
     4381 gttgaagacg gaagttttgc cggcgttggg attgccggca agcgcgattg tataacttgt
     4441 tctatcattt gtagtatcgt acattttata atcttcaaaa tttgttattt ttcacggcag
     4501 cgacgcaacc agtctcagaa agtcacctga acgcgaaaac ccaatacgac cgcatcgctc
     4561 acatcagaat ctccgccagg gttgaatata tactggatat ccggcgaaac gtgcagccac
     4621 ggcgtaagct gcatattata gtagcattcc agggctgtct catttgacgc cgtaaagtcg
     4681 gccttccggc tgagcaatcc ctggccgaca cccacgccaa gaacgtcatc atcacggccg
     4741 tctataaggc cctgatattg tacgcccgca ctccagaaat ttttaatctc gttcacatcg
     4801 ccgtcggcat aaccgtatct tgcaaaaacg cccaaacctt gtgaatcgtc ttcgacggcg
     4861 ttttccttaa aaaccatctg gtcgcaatcg atataaaatc ctgcgtcgtc ccgcttgacg
     4921 ccgttgccgt cgaatcgctc cttcgcctgc gggtcatacc acaagccaac acgatacgcc
     4981 cctggcagcc ggccctttgg ggaataaaag tcgggcataa tgcccgtctc gtaaatgctg
     5041 aatgtgttcg aaggcccatg aaacgccgta ttaaaacctg tttcgcgata atcagcatcc
     5101 gcatcgacga cggctgccgt cacgtaccac cagtcgaccg gcgtaatatg cagaatgacg
     5161 ccaaagccgg gatcggggaa aggaatcgtc ggattgttaa ccagagaatt gttcaaaaac
     5221 tgcgtgacct cgtcattggc gtacgtattg cagtcaaacg acgccggagc atcgtgacac
     5281 tcaaacccgc acgtcaaatc aaccctgccg actcgcaaaa tcatcctgtc attgaacatt
     5341 ttctgttcgt agtacaattc ccagacatct gccggctcat cgccgaactc caaaccatcc
     5401 acgccgaaaa tactgccgac cgacgaagca tcaatgccgt ccgaccatcc gccgcgggta
     5461 agcatataaa ctcttccgcc cggcagattg aaaagccggc ccatatctgc gttaagctca
     5521 aggtcatatc ggccggagta acggcccgcg cggcgatgcg tgcttaatcc gccgccgaga
     5581 ttctgctgat ataactgagt gacgttcaaa tctatcgtta tgccgctctg agcgagtttt
     5641 ctgccaagtc cgaaccagtt gtcagtaaga gtatctgccg gaagtatttt aaagggattt
     5701 atggcggatg acgactgaga ctccgtctgc gctactgacg tcgttgaagc aaaaccaaca
     5761 accatccaaa ccgccaaaaa tttatacatc cagtttcctt tcaaatgggc taatatacgt
     5821 cagccgcctt taaaaacaat agaaaattcg gcatatccca accttctgct tgattgcgaa
     5881 cacttgtctc gattcggaca ggaattgcaa tcattttcac ccgtagtgaa aagcttttta
     5941 tactcagccg acgatttgag cgattcaaaa tccggcgttt tttctatctg gaatttcatt
     6001 ctttttgccc ccagcccaca ctgcccttca agtatcattt ttaacatttc aatcgcctcg
     6061 gacgatttgc cggcagcagc caacagcatg gcgtatttgt atcgcagatt cgccggaatc
     6121 ttcgacatat caaggccatc catgattttc gacgcctcat ccttcatgcc cagcctggcc
     6181 gacactatgc cataagtcat tctggcaccc tgagtcatgg aatcaaaagt cattgccgtt
     6241 aagacgtcgt acgctttttg attttctgac gaaagcatgt atgtctcagc caacaattcc
     6301 ttcgtctcgt gagtcttaac tacagtattg agtttttctg ctatcgccat cgccggcgtc
     6361 atggcgttcc gcgttataca gtatcgccaa cagtttttta tcgccttgat atcatcggca
     6421 gaaagatgag gcttctcaag caaggcacga tgcataacgg cagcctgcag aagttcggcc
     6481 tgttttgcat agactctttt gaacttcgtc tcgacagatg cggagccccg gtaatttttc
     6541 agggcatcgt tccatttgtc cttacgaata agttcaaggc ctgcaagaaa tttctctttg
     6601 gcaattttct gatttttcaa tacgcattca ctacattgtt ctgatacttt cttcgtacat
     6661 gagccgcctt cgcagccatc acagtcggta cactcagcca tgcatattct gcccgacatc
     6721 aacccaaccg cgagaagtaa acataatact atggactttt tgatatgcgt cgtacaggtc
     6781 gacattatga tttcctttcg aaagattgtg ttcaatgacg ttcgtaatcc acacacgcgt
     6841 gcagatcata aaagaggata ttgttgttcc gtggcgactt tttagtcaat tcgtaacccc
     6901 atccgttgat attcgtccag tccttgatac caacctcagc atgtggaaag actcgtttgg
     6961 tggccaagag gcaacgttgc accgctcctc gtaccccgct attggcggga agaaaccagt
     7021 gattgtcgct aagcagtccc aagtcgaaca gttcgtctga cgtataatcg cccagccgct
     7081 tattgcccaa tacgctattt ccactgttcc aataagtatt tccgaataat gccatttctt
     7141 ccgtcgcgcc tggacatatc catgctgatg gtgccgtcgc aagcccggca ttaagtaatg
     7201 cacccatcag gccgtattgc tccggcaggc cttccttgta actgaacccc acgccgcaac
     7261 ggaagtacga ctggccgtaa taaatctttt cgccatccga atatgtaaat ccgcctgcac
     7321 ccattgcgta agggtcggga taatattcat cgttatcaca tgcataaaga tataccatct
     7381 tgccaatgcc ggaaagattt gccaggcaga ccacccgccg tgccaaagcc ttggcgcggc
     7441 taagtgatgg taacagcatg cttattaata gcgaaataac tgacgtcaca accagtagtt
     7501 caatcaagga aaatgccttt gtcatacgtt tcggatgcat ggttttgtac gataataagg
     7561 gctgttccga cccaaacatg gtgtttggac acgccggcct gaattgtaaa cccattgaca
     7621 cggtatcacc gtctacggcg aaacattgcg ccaaacgtgc ctatcgtaag aagtacaata
     7681 gtagcgggct caggaactgc cacataaaag cctatattcg ccaacgttga aatcgtactc
     7741 catgcgatat gtccgtcata ccatgcgaaa gtacctaccg aattgctagc tgcgtcgcca
     7801 gcaatcagct ctgcagctga aaatagcatc gtcaaactgt ctgcagcact tgtcgcattc
     7861 acgtcgaagc agagaatact cttcgttgtc gtctcataaa aatacacgtt gccgtcctgg
     7921 ccgtaatata tgtcgccaaa gccggcggag cttccgccgg taacagccgt tatgtcagcc
     7981 acgtcgagaa ctgtagaacc gtttgcccca tcccaactat acagcgaatc tgaatcgttt
     8041 gagccccagt acaggttacc cgaagaatca aaatcaattc cgccggaaac cgtgctgttt
     8101 ccgctgatgg cagtcaattg cgccgaggta agaaagatat cgacattgtt cgcaccgttc
     8161 gtcctgatta tgctgtcgct tgtgccctca taaacgtaat gctccccacc gtatactaca
     8221 aatgggctag ccagactaac ggcagtctgg cctgttgctg ccagaatatc tgcctgcgaa
     8281 gcatagcatg tcacttcgcc ggtattcctg ttaacgcgcc aaatagcgtc ggtagaagaa
     8341 tctgaaaatt ggatataatc gccaactatt ctgccgtcgt agatggtagc caggctggat
     8401 gtaacaccgc cgtttgcttc attccattcg gcagtcgata aaatctgcga atacgttttc
     8461 gacgtcgata cgccggaaat ttttgtaatt ctgccgctct ggccgccaga actgttcgac
     8521 aatatgcagt atgcgtcgtc atcctcgata atcacggtat agacgtttcc cgaaccttcg
     8581 gtacttcgca tatcatcaag tgaaagtatt tgcttgtaaa cggcagccat actgccgcaa
     8641 gacgtaattg ctatagcagc aataagtcca agatgtctga gtgcattcat gtgaactctc
     8701 ctgttcgtaa attacttacc tgcgagtctc cactatgcac ccactgcatc agagggattt
     8761 tattccattc aatttttcat cacggcgtat atcaggcatc acacctgcag ccggcagtta
     8821 ttgtttattc ataggcactt cctttcatac aatttattcc ttacgactgc ttctgcggaa
     8881 aatccaccac ataacgatat tgcttgccac agtggattat cacttccttc tggccacgtt
     8941 tgagaatccg cacatccatg tgaagctcgc cgaacccatc atgggctatc agttcgcggt
     9001 atagttccga tatcttcgcc agaaccggcg attcacaaaa acatgaattt gctttcatat
     9061 ggctgtacat ttctctgctc tatccgtcaa tgtgaatgaa tcatcttccc aatgtttgtt
     9121 aaggacaaca aagttcgccc agccaaatat tcatcgtaaa aaactgtttc actttttata
     9181 caatcttcgt gttaacgttc ctctgcagat ttttataaat ctggtcggga acatttttcg
     9241 ataaagttca aaaatttttt gagaaccgca tcagaaatta catgctccat tttacatgcg
     9301 gcggaatcag cctctgaata ttcgacgtcg agtattttta cgaaaaaatc ccgcaatatc
     9361 tcatggcggc gataaacttc gcgggcaata gcctggccct gctgcgtaag cgtaataatg
     9421 ccgtacggcg aataattcac gagatttctg tcagccagcg cgtgcaaagc acctgtgacc
     9481 gacggcctgc ttacgcccag ctgacggccg atatccttcg ccctggcagc ctgcttctgc
     9541 tgaatcagga aaaatatcgc ttcaagataa tcctcaaggc tatcgctgat attgatgtgt
     9601 ttcgttttcg ccattgtctc gcaccagtaa aaaactcggc taataaaatc aagtcatgaa
     9661 gaattataaa aagcccaaaa gttagtcgtg ccgaacaaaa tacttttagc cgaacttttt
     9721 gtcaagcaca attcaaaaat tttttagagt ggtgcctaaa agtgcaggta gataccaggg
     9781 aataaaaaaa ccgccgggcg gcccgacggt ttttcatttc ggataaacct gattctgatt
     9841 tgttctgttt atttattctt gccagtgccg agaatcatct tatacataag ctggtcgtcg
     9901 gcgttgtcgg cttttacggt aataacttcg ccgttgtaca accgctcaag gtctttcgct
     9961 gagaaggaga acatataagt ggtcagagtg gcttcgtcgg tagccttgaa cggatagaac
    10021 gatatcttgc ccgatgcctc tatcatgccc tgattgcttg ggattgtaag ttttacatcg
    10081 tagtccttaa tctgatttac atcatcactt cgctggaaag ttattttcgc cggccaggta
    10141 tattcatgtc ccatggtaac catttttgtt cctgtgggtt tttggaattc tatttcaacc
    10201 ggcttgtcgc agcctgcgaa aatcaacccg cccaaaaaca cggcaacgat ggcttttttc
    10261 atgacgcgct ccttaagtgt tgtgaattca gtcccttaca agccgcagcc ctcaccgcaa
    10321 ctcaacagga ctttccgttt cctatatgta atggtattcc gtaacttctt gttcttcaag
    10381 gtaaaatgta aaaatattat caggttcgcc ggctaaatga atggcagtat attcttattc
    10441 tgatttgcgt ttccccaaaa tcgccgtaaa aggcttctgt aatttcccaa cgaataagga
    10501 atcagcggca tttcagcatt tttaaacctt ttttatggaa attcaacaat tttcttcgta
    10561 gaataccgcc ttgcatccaa cgacgtatct gccgcacaaa agagaggtta tccgatatag
    10621 gggagtgatt tgtttaaaca ttttttcaga aaaatgcttt tgaaacttgc aaaaaggcag
    10681 acaattaata aaattccgta ctttcagagt aaccacggct gttatccgca cgggcggaat
    10741 gtgtggaaga tgttttagaa atgtatgata gtgttacagg cgtttgaatt cctggcagcc
    10801 cacgtggggc cagccgctca aacgcctcgc aattaatttg aactacatag agtgtgttca
    10861 ggaaaggtca gaaacgatat gcttacgctc ttttggaaga atgctacgca caaacgcgtg
    10921 ggagcccgca acggagtcac cccgacggac ctggcagaaa tcgaaccgcg tcttcgcaag
    10981 gcgcacgaag cagtcgtctc gcaatgccac gacggaaagc tcggctacgc gactctgcca
    11041 acgaacgaaa catacagaaa acaggtaacc gacctggtgg cacgttataa agaaaacacc
    11101 accgacatgg tcgttctggg cataggcggc agtgcgctgg gcaatattgc attgcagagc
    11161 gcattaaatc catcgacata taacattctc actgagcgca agcgcaaagg cccacggctg
    11221 ttcgttctgg acaacgtcga cccatgctac gtacacgacg tactcaaatt cgtttcccgc
    11281 agggcgtctt ccacacttgt aaacgtaatc agcaaatccg gcgaaacggc agagaccgcc
    11341 agccagttca tgctgttccg tcagatgctg cgtgacaaga ttggcgataa cttcgcgtct
    11401 cgtatcgtag ccaccaccga tgcgtcgaag ggcactctgc acgacatagc caaaaaggaa
    11461 ggctacgcca tgctgcccgt accaggcgac gtcggcggcc gtttcagcgt gctttcgccg
    11521 gtcggcctct tcagtgcagc catgtgcggc atcgacatcg acaaactact cgaaggcgcc
    11581 aacgccatgc ggcagcgggc ggaagtcatg gacgtgatgc agaacccagc caccatcctg
    11641 gcagccatca agtacatcat gttcaccaag aaaaagaaac ccatgcatgt gatgatgtcg
    11701 tattccaacc gcctgtacat gctggcggac tggtatcgcc agctgtgggc cgaaagcctc
    11761 ggcaagcagt acgaccgcga aggcaacgaa gtcttcaccg gcccgacccc aatcaaagcc
    11821 ctcggcacaa ccgaccaaca ttcgcaggta cagctctacc gcgaagggcc gaacgacaaa
    11881 ttcaccgtct tcctcgacgt aaagaaacag cccgaagaca tccgcgtacc caacacattc
    11941 gacgatattc agggcatgag ttacctgcgg aaaaaacgca tgagcaaact cctgcaagcc
    12001 gaaaaactcg ccaccgaata cgccatgacc gtttcgcaac ggcccagcgt cacgctgact
    12061 tttcctgaaa tcagcgaacg ctacatcggc gaattcctgt tcctgtacga attcacaacc
    12121 agcatcatgg gcgaattgat gaacatcaac gcttatgacc agccggctgt cgaactcggc
    12181 aaaaaagcca ccttcgccct catgggccgc aaaggctacg aagaactcgc cgaaaagatg
    12241 aaatccttcg tccgcatgga cagacgctat caggcgtaat taacactgat tcgcacatta
    12301 aaaaacggac ggctttcaat cgaaggccgt ccgttttttt gaatgactct ctcaaaaaat
    12361 tttcatttcg agaatttttt tatactcatt ttacaccgcg ttgagttccc tgtatttgtc
    12421 ggcgattatt acgacaacca tgaaaaatcg gctcttttgc agccatagca acgcccatgt
    12481 ttcggggatg gattctaaaa agctcagagt ttgatgaatt ccgcaacttt tcggaaaaat
    12541 ccatcaaggt cgccatcgac gaaatatctg ccgtccgccg gctcgaatgc gaccggcccc
    12601 ctgttcacta ttacgacctc accgccggca atatgcggca gcatagcggc tggatagacc
    12661 accagcgaag aaccgagcac gaagaacagg tcggactctc tggcggcacg ctgggcgtcg
    12721 aatatccggt tgactgcttc gccgaaaaat actacgtccg gcttgattat gccacgttta
    12781 ttgcaatcgc accgcgggat gtccatctcg ccgagcatgc gttccaggtc agccagcgaa
    12841 tattcacgcc cgcactcaag gcagtgcgac gtgttgtaat tgccgtgaat cggtatgaca
    12901 tttttactgc cggcagtata gtgaagaggg tcgatattct gcgttacaac cgcagacagc
    12961 atgccggccc gttccatgtc agccagaaat ctgtgcgtga acgtcggaga aagcgtatcg
    13021 acaatcgcca gcaaatcccg agtgaatctg taaaattccg acgggtcgcg ataaaaataa
    13081 ttgatatcga acacgacatc tgcattgtag cggccagagt tgtatatgcc cgtcgggctg
    13141 cgaaagtccg gtatgccggc tgccgtcgat atgccggcgc ctgtcagcac tgcgatattt
    13201 ttcgcgtttc ggatttttgc cgcgcaaatt tcaggtgtaa tagtttgttg catgatagtc
    13261 aaaaaactga agtttctaac aaaatgaatc aaggcacgga ttttttaaaa aaccattcac
    13321 aaaacctcgg ctgcctgcaa tcggcaattt tggcgacacc cgtattgagg gatggattct
    13381 agaacctatc ccaaaaacct tggctgcctg caatcggccc ttttgcagcc tggcggcgtc
    13441 agaaaaacgg cagaatatcc ttaaatatgc tgccattttt cttccttgcc agcccgcaaa
    13501 attgctcgat ttcggcgacg cccatgtttt tgggataggt tctagaaatt cttaacagac
    13561 atcctgaaaa atcaatcaag attcttcagc agatagcggt tgaacatttc cgtcggaaat
    13621 gccttgcctg gctgatcgct ggacggatcg agctgattgt tgatgtaaac gcaatctgcg
    13681 gcaatgccgc aacgtttctg aagtttgttt acgagcgtca ccagggcgtt gaactgttcg
    13741 cggccagggc ctttggcgga aaaatcgcct atcaggcaga tgccgatgct gtcgctgtcg
    13801 aaatcatggc cgggtatcat cacatgataa ccacgaacct gcctggtcca caaatcggtt
    13861 gcaaccacac ccttgcccga cgtattagca tcggcggata tgatgaaatg gcatcgttgc
    13921 gggagagaat cgttcatatc cgttgcggaa tgaaccacaa tcctgcgcca cgtgtgggtc
    13981 aggcccatct gggtataatc aactttgccg ataatgctgg cggaagtggc attctgagga
    14041 ggcatggaat ccatggacat aagaagaagg gcaccgccgg tcatggcgac cagcagtgat
    14101 aacaacactt taactgtcct gccgtcaatc atcacgattc ccagtattgt ctctcttacg
    14161 gtaagcagca cctttatggc ctcctaatct aaactcttta tcggcaaagt tcaagcaatt
    14221 ccaacagaag ttttacaaaa actttttgta tttcaactac tttaaaaaca agcacttgca
    14281 atatttatcc acagcttgtc cacaggaaaa ttttattgac taaggcattt accttacaat
    14341 atttacggta aaaatttaca aaacatccag ggctgccggc taaatgcatt ccatttcgta
    14401 acattgcgat gaaaagatgt taaaatatga ttcgcaaaag attttttttg aaagttcaag
    14461 caggccagcc tgctttttga ttgtcgatac agacggacgc agtcaaaaaa aaactggcaa
    14521 caagctcaaa atcttttaca aaataatctt ttgcagcctt gcaaaaattt tgaattggta
    14581 ttagccggct gcgacaatat aatgttttgc ccgtagttac ataacaggaa taaaagatga
    14641 agttaccggt ttatctcgac aataacgcca ccacgccagt cgaccctcgc gtgctggacg
    14701 ccatgctccc atatctcaag gatcacttcg gcaatgcagc cagccgttcg catagcttcg
    14761 gcctggaagc gtcgcacgcc gttgaaaacg cccgccagaa tatcgccgac cttcttcaca
    14821 ccgagaccaa ggaagaaatc gtcttcacgt ccggcgcgac ggaaagcgac aatctcgcca
    14881 taaaaggcat tgctgaaaaa ctcg
//
LOCUS       DAONFI010000010        14503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_4507_length_14503_cov_3.664037, whole genome shotgun sequence.
ACCESSION   DAONFI010000010 DAONFI010000000
VERSION     DAONFI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4507_length_14503_cov_3.664037"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLK08_00755"
     CDS             complement(<1..617)
                     /locus_tag="PLK08_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPS52057.1"
                     /translation="MKSVIYDNPAYWNLLWVVLILAIVVAVGITWRRRALTRFVGNIF
                     AGLLAPGVSLTRLRIKGWLWVLAMVALVLALVDIRWGKHMVEVQQKGVDIMFVLDVSR
                     SMLAEDVTPNRLMRAEEYIGDMLDDMKGDRAGLVVFAGSEKLLVPLTGNYSEIKMALQ
                     EAGPQSVVRGGTALGDAIRVSARSFLDKTPDYKAIVVFSDGEDMD"
     gene            complement(728..1822)
                     /locus_tag="PLK08_00760"
     CDS             complement(728..1822)
                     /locus_tag="PLK08_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPS52058.1"
                     /translation="MMSWHNAWFLLLLMPVALIAWRMFLPRKWAAVGFSDTGQAAKIS
                     GGLKAGFMWLCPALKILGLVLLVICLARPQRGIEKTKIFTEGIAIEMVLDRSGSMQAL
                     DFTLNNRRVDRLDAIKNVATRFIEGDKALNLPGRQHDIIGMVTFAHYADNLCTLTPDH
                     AYLIKKIDEIKIVNTQDEDGTAIGDALGLAVERLDTLKSKKKPDGTDRIKSKIIILLT
                     DGENNRGDIMPLKAAELAKSHGIKIYTIGVGTKGMAPVPVIDPFTGQTVIRLAKVDID
                     EKTLKEIARLTGGEYFRATDTKSLEKIYATIDKLEKTVIEQKKYAEYRELALKPFRIG
                     GYMFPPLLLVAFVVLGLEALLANTWLRKVP"
     gene            complement(1819..2820)
                     /locus_tag="PLK08_00765"
     CDS             complement(1819..2820)
                     /locus_tag="PLK08_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52059.1"
                     /translation="MRSIWFAILVFAVAAGCITGGCSRDRKSVEQLPPPIVKTAEKGP
                     VKLTVTLDRSEAQVAWPLRLTIAAVAKNGVTVTLPNAVKTLGDMTVTQTADDPDIPTA
                     DGTARLWKRTMTLESYTSGEKNIPAVTVKFTDLRDPKKPVKTELTTEAFKLKIISAIQ
                     GKADPYKPRDIKGTLELTPKPDYSWVWIALCVIAGLAAVLLVVRYFIRRRIMRALTAG
                     EWAMRQLNILEDEHLPETGLVEKFYVRLTSVVRQYIERRFGIAAPKLTTREFLEKVQA
                     RHIVSDDNRQSLQQFLSAADMVKFAKFTPNPDEVNSSFGTARDFISRTAARSEEEVR"
     gene            complement(2934..3818)
                     /locus_tag="PLK08_00770"
     CDS             complement(2934..3818)
                     /locus_tag="PLK08_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPS52060.1"
                     /translation="MVSTSELLKKVRRIEIYTSHIVDDILSGQYHSAFKGRGMEFEEV
                     RPYAIGDDVRTIDWNVTARHNQPFVKLFREERELTVTLLVDLSASQGFGSGEQLKHEL
                     VAEVGATLAFSAIKNNDKVALIGFTDEIEKAVPARKGTRHVLRVIRELLGCEPKGVKT
                     SIARALEHLSRVQKRRSVVFLISDFLDSGYEKALRVARRRHDIVPIVVSDPREDELPN
                     VGLIELRDEETGQHVLVDTSRGRNRRQYAQLVTQRNKTRDDLFRRLNMEAIYLKTGQD
                     FIDPLKAFFHKRLMKLKR"
     gene            complement(3844..4839)
                     /locus_tag="PLK08_00775"
     CDS             complement(3844..4839)
                     /locus_tag="PLK08_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPS52061.1"
                     /translation="MSTHEQITEMSDRVKEASSHFRRLVDEVSHVIVGQERLLHRMLI
                     GLLSNGHLLIEGVPGLAKTLAVSCLSKGIDTGFQRLQFTPDLLPADLTGTQVYHPQTQ
                     TFVVQKGPIFSNLILADEINRAPAKVQSALLEAMQERQVTIGGETFKLPQPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVIVGYPSRQEERKILDRMGSTAPDIDINAVMKPDDI
                     IAARKLVDEMYVDDKVKDYIVDLVVATREPGTFGVPVEGLIDYGASPRATIAITLAAK
                     ANAFLAGRGYVIPQDVKDLAMDVLRHRVIVTYEAEAEGKTSEDVVRTILEYVPVP"
     gene            complement(5198..6082)
                     /locus_tag="PLK08_00780"
     CDS             complement(5198..6082)
                     /locus_tag="PLK08_00780"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPS52062.1"
                     /translation="MKYQKISDIFKDGRKTLSFEFFPPKTDKGREQLYRTAEELMTLS
                     PDFFSVTYGAGGSSARSTLEIVETLQSKFDIPVMHHFTCTKHTRVAIRNELNELMASG
                     IHNILALRGDPPAAEPHYEPGPDEPRYAFELIKLIRERAGWFSVGVAVFPEGHCQTPS
                     RELDSLYTRVKQEVGADFGICQVCFDIEIFREFYMRQCDAGVTMPMIPGILPVVNWEN
                     FVNFCNRCATKIPEILKPIYEPIAADTAAMEKAGVDFSVELCRSLLDINVPGLHMYCL
                     NKPEPISTVVHTLGNDWR"
     gene            complement(6392..6733)
                     /locus_tag="PLK08_00785"
     CDS             complement(6392..6733)
                     /locus_tag="PLK08_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPS52063.1"
                     /translation="MHTITKKELVDRIADRTEQKRVVVKRIIQMFLDEIIDELGQGNR
                     LEFRDFGVFETKSRAARMAQNPKTLQRVHVPPKRTVKFKVGRLMKLKLLGEELPPRDY
                     NSKEFEENDNV"
     gene            7071..8846
                     /locus_tag="PLK08_00790"
     CDS             7071..8846
                     /locus_tag="PLK08_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52064.1"
                     /translation="MKKLAVIALFVSIIASLHGVAWSQYQRPNVSYEAAGQLASLARS
                     AMTDYLHKRTPADKYPIPDSLSMFDSDAYDYTVFITLRRDGVKLASGSASGRGLARNT
                     VAAALKAMRSSALGDRVTDDTLTKLAVEIEILGQMQVIPEQDVTAKLIPGLLGLRFWS
                     GIDDPKLIGLLKANNIDVTILPSEEYRNGWTPLQIREKILETVSKTDVSLLPDSKPKW
                     QIFSSLHFLNPADTKECYIYYRGKILRVYPGNKYFLVTLLNASARNFDMAESSVKNPD
                     GTSAPLWQELYLAYVMRKVAAKFPGKIRKLSTDIANAILAKVAKERVRFDHRMNIAYV
                     MADPTEDEILSTAFLSLAVAGDNSNPEIVSLSKAIRNFLFMRIDNNGVITDSRERKIG
                     NLAFAVALRAIAQEKMTPQQTMIFCKSLDTLLGRAGEMGSSIKQGDSLDLTATAWLAR
                     TIIAGGAEIQTQYLTFLKRFTKRLIDAQPEFSQLLDENGALIDSRGQADTVATATAVC
                     VFMEMQGVAKWAGVSAASRNAIDFCWLMSYRPHEAYFAADQAKWPGAVRINPAAAKTS
                     LAASAAVLDAITDIMAPESETDKKS"
     gene            8849..9595
                     /locus_tag="PLK08_00795"
     CDS             8849..9595
                     /locus_tag="PLK08_00795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPS52065.1"
                     /translation="MDIPRIFNIAESAHRIHNPFTSEKLAAFGAALRLERGTLVLDLG
                     SGSGEMLCTWARDYGILGIGVDMSRLFSEQAELRAEELGVADRVEFIHGDATGYVADE
                     KVGVAACIGATWIGGGVAGTIKLLAKSLRSGGIILVGEPYWLRLPSTEDTAKGCGADS
                     IADFLTLPELIASFGELGYDVVEMVLADQESWDRYEAAKWLTMRRWLETNPGDDFAKE
                     VRAKLTAEPKRYVTYTREYLGWGVFALMAR"
     gene            9843..10379
                     /locus_tag="PLK08_00800"
     CDS             9843..10379
                     /locus_tag="PLK08_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017014962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52066.1"
                     /translation="MALKYLNLDETTRKFMLQEVDLDFNSEKGLNMSRNFNEEGIRNW
                     GDCLKTAIEMYDDAWLANQLRDNNYMKEYTERRKPKGGFTMAKVPVTAPEMFAEGEFN
                     RFYARGLCLRAIEEGILNVIVYRAKDVAHSRPESEAKIGTSIDANVLLNDLRTSQGED
                     TERGIPGGPNSGLSIKLP"
     gene            10520..11815
                     /locus_tag="PLK08_00805"
     CDS             10520..11815
                     /locus_tag="PLK08_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52067.1"
                     /translation="MSRRAKNTLFRRGAFTACHAELSSIIKANGKSLRIFFRGLFNRP
                     GIKYNPAMGRKRKKILGKNHTSLAIDEWAVSRRIHFIIAMMAQIVVASAESMYTACRD
                     ARVNAKYGGNVEPPYPSDDHWMNFYNNPDKPLVTAMELVIPSKDWFTVLLGLDSQQID
                     ALNEQFHRIQENSDNLSPQDSFSHLLMLSNNYANIIYRNHILKLRDAIAGIDDGSPEQ
                     IKAVKASPEILFMIRVWVPCFLEYKISFDELLTQARTGDIEAIEKIIRLDRGTASCDP
                     LIQTHFLQAKRQNRKAWVERYTKALNADEPLGVFSSQHIKYLLAGYIYEMSDIFSKAL
                     DKCYRSIKLKFKRGCKPKSKLSYEDILSLFHCYAADSYPKNIKQLPIIKDVDFLSIDN
                     KSFETRVRETRKIWHEILVSLIAEVNEEATKCQAESAVQ"
     gene            11910..12830
                     /locus_tag="PLK08_00810"
     CDS             11910..12830
                     /locus_tag="PLK08_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52068.1"
                     /translation="MVYAQGRILKVKVPHSSQTANTPGLRGKIIEFSAQSRKRMIDLL
                     ARIDTDNAGFVCFITLTYPDLDGPPSHEITERDRRTFLKRIARRFPESSAIWRREHEP
                     RKSGEQAGAIYPHYHMLFFGMPFFDCDELNAIWREIMNYPDYVRTEIKGIKSWRQAMY
                     YLSKYMAKIARQGDGSQPDGQAPAGEAGTDDGGNAAGSLVYGTYLTGDNKINNEPGKK
                     LLPVGRAWGVFNRHNLPLAELHSASLPAGQWMEAVKDAAAQVWPGVNAVDGYGFTLFL
                     DNPTRSKQWFDGISKLANVLPLKPDDGIPF"
     gene            12842..13027
                     /locus_tag="PLK08_00815"
     CDS             12842..13027
                     /locus_tag="PLK08_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52069.1"
                     /translation="MQKTAQQISLPPGTPDWITLGLIAETIDTWQPYYPKSLTPADAV
                     EIIQSMGKLMYTLQGKH"
     gene            13042..>14503
                     /locus_tag="PLK08_00820"
     CDS             13042..>14503
                     /locus_tag="PLK08_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPS52070.1"
                     /translation="MTTKNSYLALARVSSREQEREGFSLDVQEDALRRYAERHGGEIV
                     KFYRIAETASKQAERKTFKELLEYAKKNAHKLDGLLFYKVDRAARNLFDYVELERLEM
                     KYNLPIVYVAQPTENTPAGRMQRRILANMASFYTEQQSLDVREGQSRRAESGLFPCKA
                     PYGYRNNRVDGRSLVEIDTDAAANVRRIFELYSQHGQTLDGVVQAMAADGRTYTSTQP
                     QFTRSKVHHILRDRSYIGEVLYRGQWLAGKHSPLIGREVFDRVQVLLGDKTYASHDSV
                     YGSGMVKCGHCGRPMVYEVKTKSTSSGPKEYRYYRCSRYTSAGHPRVRINETQLDKQI
                     IEFFRQMKVSDPKIHAWMVKIIQLKAETFQRDDEKRLDDLQRQMAVANRQRSKLLNMR
                     IAEEIDRDTFAAKDAELRDQMQHLSVLIDAHSREKSEYADIAIKAFELSQQLENKWLT
                     GDVAAKREILNILCLNYTLNDVSLDMEIRKPFDVLAK"
BASE COUNT         3698 a         3730 c         3565 g         3510 t
ORIGIN      
        1 ctgtccatat cctcgccatc ggagaaaacc acgattgcct tgtaatcagg tgtcttgtcg
       61 agaaaactgc gggctgaaac cctgattgcg tcgccaagcg cagtaccgcc gcgcacaacg
      121 ctctgcgggc cagcctcctg aagagccatt ttgatttcac tataattgcc cgtaagcggc
      181 acaagcagtt tttccgaacc ggcaaaaacc accagccccg cgcggtcgcc tttcatgtcg
      241 tcgagcatat cgccgatata ttcctccgcc cgcatgaggc gattgggcgt cacatcctcg
      301 gccagcattg aacgcgacac gtccagcacg aacatgatat ccacgccttt ctgctgaacc
      361 tccaccatat gcttgcccca tcgtatatcc accagtgcaa gcacaagcgc aaccatcgca
      421 agaacccaca gccaaccctt gatgcgcaat ctcgtaaggc tgacccctgg cgcaagcagg
      481 ccggcgaata tgttgccgac gaaacgcgtc aacgccctgc gacgccacgt tatgccgacg
      541 gctacgacta tcgccagaat caataccacc cacagaagat tccaatacgc cggattatca
      601 tatatcacgc tcttcatgtt cagtaaaaat cctctcggtt attttctgca acaatccgtc
      661 tgcaagccgt tcaacgtcac taatcgattt cagcctcaaa aacaggttca acagattgac
      721 gattttttta aggaactttt ctcaaccagg tattcgccag cagcgcctcc aggcccagca
      781 cgacaaacgc caccagcaac aacggcggaa acatataccc gccaatcctg aaaggtttta
      841 gggcaagttc acggtattcc gcatattttt tctgctctat gacagttttt tccagcttgt
      901 cgatagtggc gtagattttt tcgagtgact tcgtatccgt cgcgcggaaa tactcgccgc
      961 cggtcagccg tgcgatttcc ttcagcgttt tttcgtcaat atcaacctta gccagtctta
     1021 tgacggtctg gccggtaaag gggtctatca ccggcacggg ggccatccct ttagtgccga
     1081 cgcctatggt gtaaatcttg atgccgtgtg acttggcaag ctcagccgcc ttgagcggca
     1141 ttatgtcacc gcgattgttt tcgccgtcgg tcagcagaat gatgattttg ctcttaatcc
     1201 tgtcagtgcc gtcaggcttt ttcttgcttt tgagcgtatc cagccgctca acggcaagcc
     1261 caagcgcatc gcctatggct gttccgtcct catcctgcgt attgacgatt ttaatttcgt
     1321 cgattttctt tataaggtat gcatggtcag gcgtcagagt gcagagatta tcggcataat
     1381 gcgcgaacgt gaccatgccg atgatatcat gttgacggcc cggcagattc agagccttgt
     1441 cgccttcgat gaatcgcgtc gccacgtttt ttatggcgtc cagccggtcg actcgccgat
     1501 tgtttaacgt gaaatccagc gcctgcatac tgcctgacct gtcaagcacc atctctatgg
     1561 caatgccttc ggtgaaaatt ttcgtcttct ctatgccgcg ttgcggccgc gcaaggcaga
     1621 taaccagcaa caccaacccc agtatcttca gcgcgggaca cagccacatg aatccggctt
     1681 taaggccgcc ggaaattttt gctgcttggc cggtatcgct gaacccaaca gccgcccact
     1741 tacgcggcag aaacatccgc catgctatca gcgccacagg catcagcagc aacagaaacc
     1801 atgcgttatg ccaactcatc atctgacctc ctcttcactg cgggcagccg tgcgcgatat
     1861 gaaatccctg gcagtaccga acgacgaatt cacctcgtca ggattcggcg tgaactttgc
     1921 gaacttcacc atgtcggctg cggaaaggaa ttgctggagg ctttgacgat tgtcgtcgct
     1981 gacgatatgc ctggcctgaa ctttttcgag aaattcacgc gtagtgagtt taggcgcggc
     2041 gattccgaac cgtcgttcga tatactgccg cacgacggag gtaaggcgta cataaaattt
     2101 ttcgacaagg ccggtttccg gcagatgttc atcttcgagg atatttaatt gccgcatagc
     2161 ccactcgcct gcggtaaggg ctcgcatgat tctgcgacgg atgaaatatc gcacaaccag
     2221 cagaaccgcc gccaagcctg caataacgca cagggcaatc catacccacg aataatccgg
     2281 cttgggcgta agttcgaggg tccccttgat gtcgcgaggt ttatacggat cagccttgcc
     2341 ctggatggcg gatattatct tcagtttgaa cgcctcggta gtgagttccg ttttcacggg
     2401 ctttttaggg tctcgaaggt cagtaaactt caccgtaacg gctggaatat ttttttcgcc
     2461 tgacgtgtag ctctcaagcg tcatggtgcg tttccacagt cttgcagttc cgtcagcagt
     2521 cggaatatca gggtcgtcgg cagtttgagt aacggtcatg tcgccaagag ttttaacggc
     2581 attcggcagc gtaactgtta cgccgttttt cgccacagcc gctatggtca gacgcaacgg
     2641 ccaggctacc tgcgcttcgc tgcggtcgag tgtaacggtg agtttgacgg ggcctttctc
     2701 ggctgttttg actatgggcg gcggaagctg ttccacagat ttcctatcgc ggctgcaacc
     2761 gcccgtaatg cagccagccg caacagcaaa aaccagtatt gcaaaccaaa tgctacgcat
     2821 attttctcct gcgttgccgg aatctcacaa aagcaaaaac cggacaacat cgaatacaaa
     2881 actcatgcct gaatccgtcg attcaccatt cgcaaccata aatctgcaat cactcagcgt
     2941 ttgagcttca tcagccgttt atggaaaaac gccttcaacg gatcgatgaa atcctggccg
     3001 gttttgagat atattgcttc catattcagc cgccggaaca ggtcgtcccg cgtcttgttg
     3061 cgctgcgtca caagctgtgc gtattgtcgc ctgttgcgac ctctcgacgt atcgaccaat
     3121 acatgttggc cggtttcttc gtcccgcaat tcaatcagtc caacattcgg cagttcatct
     3181 tcccgcgggt cggatacgac tatgggtacg atatcgtgtc gccggcgcgc cacgcgaaga
     3241 gccttttcat aaccgctgtc gagaaagtcg ctgatgagga aaaccaccga gcggcgtttc
     3301 tgaacgcggc tgagatgttc aagcgcgcgg gcgatggacg tttttacgcc cttcggctca
     3361 cagccgagca gttcgcgtat cactctaagc acgtgtcgcg tgcccttgcg agccggtaca
     3421 gccttttcta tttcatccgt aaaaccaatc agcgcgacct tgtcgttatt tttaatcgcc
     3481 gaaaacgcta gcgttgcgcc gacctcggct acgagttcgt gtttgagctg ttctccgctg
     3541 ccgaacccct gcgatgcgct caggtcaacc agcagcgtta cggtaagttc acgttcttcg
     3601 cgaaacagct tgacgaacgg ctgattgtga cgtgctgtga cgttccagtc tatcgttcgc
     3661 acatcatcgc cgatggcata cggccgtact tcttcaaact ccatgccgcg gcctttgaac
     3721 gcggaatgat actgaccgct gagaatgtcg tccacgatgt gcgacgtgta tatctcgatg
     3781 cgccgcactt ttttcagaag ttcgctggtg ctaaccattg tccagacctc cagactccaa
     3841 cgcctacggc actggcacgt attccagtat tgtccgcaca acatcttcgg aagttttgcc
     3901 ttcagcctcg gcttcgtagg tgactataac gcgatgacgc aaaacgtcca tagcgagatc
     3961 cttcacatcc tgcggaatca cgtaaccgcg tccggcgagg aatgcgttag ccttggcagc
     4021 cagagtaatt gctatggtag cacgcggcga ggcgccatag tctataaggc cttcgaccgg
     4081 cacgccgaac gtaccgggct cacgggtcgc caccaccagg tcgactatat aatccttgac
     4141 cttgtcgtca acgtacattt cgtcgaccag cttgcgggcg gcgatgatat cgtcaggctt
     4201 cattactgcg tttatgtcga tatccggcgc agtactgccc atgcggtcga gaattttccg
     4261 ttcttcctga cgcgacggat atccgacgat tacttttagc ataaaacggt ctacctgcgc
     4321 ttcgggcaac ggatacgtac cttcctgctc gattgggttc tgggtagcca tcacaagaaa
     4381 cggctgcgga agtttgaagg tttctccgcc gatggtaacc tgacgttcct gcatggcttc
     4441 gagaagagcg ctttgaacct tggccggcgc acggtttatt tcatccgcaa gaatgagatt
     4501 cgagaaaatc ggacccttct gcactacgaa cgtctgcgtc tgcggatgat atacctgcgt
     4561 gccggtaagg tcagccggca gaagatcggg agtgaattgc aaccgctgaa aaccggtatc
     4621 tatgcccttg gaaagacatg atactgcaag ggtcttggcc aggcccggca caccttctat
     4681 aagaagatgg ccattgctca gaaggccgat gagcatccgg tgaagcagcc tttcctggcc
     4741 gacaatgacg tgcgaaactt catcgacaag acgtctgaaa tgcgacgacg cctcttttac
     4801 cctgtccgac atttccgtaa tctgttcatg cgtactcatt taccgcacct cccgtgcttc
     4861 ctgtcaaaat gtccaaaaca taaataacca tacgcggccg attttattgc atggtttgca
     4921 accgctttat acgctatcaa aatcagccga tttaattgga aactgcaata aagcaacatt
     4981 atggatttat acgtagccgt ttacggcttg gttcacattt ttcaccgaca gccctaaaaa
     5041 atccctgccg gagttgcctg aaaaattcag accccgtatc aagaaaccct tacaattttt
     5101 gaaaaacaga acttttctcc gataaaccat gaaattttac cgcttcgata taaaaatcac
     5161 cgttgatttg acaagcggaa atcaaacgat tatttcatta tcgccaatca ttgcccagcg
     5221 tgtgtaccac agttgagatg ggttcaggct tgttcagaca atacatgtga aggcctggaa
     5281 cattaatgtc aagcaggctg cgacagagtt cgacgctgaa atccacgccg gctttttcca
     5341 tcgcggctgt atccgcggcg atgggctcgt aaatcggttt gagtatttcg gggattttcg
     5401 tggcacatct attgcagaaa ttaacgaaat tctcccagtt tacaaccggc agtatgccgg
     5461 gaatcatcgg cattgtcacg ccggcatcgc actgccgcat ataaaattcg cggaaaattt
     5521 ctatgtcgaa acagacctgg catatgccga aatccgcacc gacctcctgc tttacgcgag
     5581 tgtaaagcga gtccaattcc cgcgaaggcg tctggcagtg gccttccgga aaaaccgcca
     5641 cacccaccga aaaccagcca gcccgctcgc ggataagttt tataagttca aatgcataac
     5701 gcggctcatc agggccaggc tcataatgcg gctcagccgc cggcgggtcg ccccgtaatg
     5761 cgagaatgtt gtgtatcccg ctagccatta actcgttaag ctcgttacgt atagccactc
     5821 gcgtgtgctt ggtgcaggta aaatggtgca ttacgggaat gtcgaatttc gactgaagcg
     5881 tttcgacaat ttccagcgta ctcctggctg acgaaccgcc ggcaccataa gtaacggaaa
     5941 aaaagtcggg cgagagcgtc atcaactctt cagccgtcct gtaaagctgc tcacgccctt
     6001 tatccgtctt gggcgggaaa aattcaaacg acaaagtctt tctgccgtct ttaaaaatat
     6061 ccgaaatttt ctggtatttc atcgattctt ccgaaaatag caaacaaaat gcttctgccg
     6121 gctacatctg cctgcatttg aatatcttac gtgcagatag ggaaaaaggc aaaaaatttt
     6181 tacctttttg aatatttcac gacggcatga acgattccag attagagcat gttaaacttg
     6241 agatgctcta gaatcgcctc tctatatccg ttcggtctta aaacaggaca ataccgccgg
     6301 cagccatgaa aaatgcgccg ccagattaac cggcggcgca tttggaaaga caaaaaatca
     6361 aaccgacatt ctttatgcac attaatatgt gctatacgtt gtcgttctct tcaaattctt
     6421 tggagttata atctctcggc ggcagttctt cgcccagaag cttcagcttc ataagcctgc
     6481 cgaccttgaa tttgacggtt cgtttcggcg gcacatgcac tctctgaagg gttttgggat
     6541 tctgagccat cctggcggca cgcgatttgg tctcaaaaac gccgaaatca cgaaattcca
     6601 gcctgttgcc ctggccgagt tcatcaataa tctcatccag gaacatctgg ataatacgtt
     6661 tgaccaccac ccgcttctgc tccgtgcggt cagcaattct atcaaccagt tcttttttag
     6721 taatagtgtg cataaatgta cctttcgttt cgggccgctt tgggcattga tcgaaaaaat
     6781 gtacatctcg acggttttat accgcttttt caaccctgtc catggactac gccaaatccg
     6841 ttaaaaaatc gtccaaaaac atcaattatg caaaattttc atattgccta accatagtct
     6901 cgcctgaaca gcacgctaca tcgtcgacat ctattataga actctccacg tcgacaagac
     6961 ttacaatata ttataagcac ttgccaacgt gaacaccaaa taaaaaatga tttttttgag
     7021 ttttatctca gggctttaca tttcgccatc aaggccgtaa actaccatcc gtgaaaaaat
     7081 tagcggtcat cgcgttgttc gtaagtatta tcgcctcact tcatggtgta gcgtggtcac
     7141 agtatcagag gcccaacgta agttacgaag cggccggaca gctggccagc cttgcccgca
     7201 gcgccatgac cgattatctg cacaaacgaa cgccggcaga taagtatccg atacctgata
     7261 gcctaagcat gttcgattcc gacgcttatg actacacggt tttcattacg cttcgtcggg
     7321 acggcgtgaa acttgcatca ggcagtgcaa gcggcagagg tttggcccgt aacaccgtcg
     7381 cggcggcact caaggcgatg cgcagcagtg ccctgggtga cagggtcacg gacgatacgc
     7441 tcaccaaact ggcggttgaa atagaaattc tcggccagat gcaagtcatt ccggaacagg
     7501 acgtcaccgc caagctgatt cccggcctgt tgggcctcag gttctggtca gggattgacg
     7561 accccaaact catcggcctg cttaaagcca acaatataga cgtcaccata cttccttccg
     7621 aggaataccg caacggctgg acgccgctgc agatacgtga aaaaattctc gaaaccgtct
     7681 cgaaaaccga cgtttcgctt ctgcccgact ccaaacccaa gtggcagata ttcagcagcc
     7741 ttcattttct caatccagcc gacacaaagg aatgctacat atattacagg ggtaaaattc
     7801 tgcgagttta ccctggcaac aaatattttc tggtaacgct cctgaatgcg tcagccagaa
     7861 atttcgacat ggcagagagt agcgtaaaaa atcctgacgg cacaagtgcg ccgctgtggc
     7921 aggagctata cctggcgtat gtgatgcgta aagtggcggc aaaatttccc ggcaagatac
     7981 gcaaactctc aaccgatatc gccaatgcca ttctggccaa agtcgccaaa gaacgcgtac
     8041 ggttcgacca cagaatgaat atcgcctacg taatggcaga cccgacagaa gacgaaatac
     8101 tttcgaccgc ctttttgtcg ctagccgtgg ctggagacaa ttcaaatcct gaaatagtgt
     8161 ccttgagcaa ggcaatccgt aattttctat ttatgcgaat agacaataac ggcgtcataa
     8221 ccgacagccg cgagcgtaag ataggcaatc tcgccttcgc cgtcgctcta cgggcaatag
     8281 cccaggaaaa gatgacgcct caacagacga tgattttttg caagtcgctc gacaccctgc
     8341 tcggccgtgc cggcgaaatg ggcagttcaa taaaacaggg cgattcactt gatttgaccg
     8401 caacagcctg gttagccaga accataattg ccggcggtgc ggaaattcag acccaatacc
     8461 tgacatttct gaaacggttc accaagagat tgatagacgc ccagccggag ttttcgcagt
     8521 tgttggatga aaatggtgcg ttgatagatt cccgcggcca agccgacact gttgccacag
     8581 caacagcagt gtgcgtgttc atggaaatgc agggtgtggc aaaatgggct ggcgtatctg
     8641 cagccagtcg aaacgcgata gacttctgct ggctgatgtc atacaggccg cacgaggcgt
     8701 atttcgcagc cgaccaggct aaatggcccg gtgcggtacg aatcaatcca gccgccgcca
     8761 aaacatctct tgccgcatca gccgccgttc tggacgccat aactgacatc atggctccag
     8821 aatcagaaac agataagaaa tcatgacaat ggacatcccg cgtattttca acattgccga
     8881 gagcgcacat cgcattcata acccgttcac atcggaaaag ctggccgcgt tcggcgcggc
     8941 gctgcgtctg gaacggggaa ccctggtgct cgacctcggc agcggttcgg gcgagatgct
     9001 ttgcacgtgg gcacgcgatt atggaatcct cggcatcggc gtcgacatga gccggttgtt
     9061 cagcgagcag gctgaactcc gcgccgaaga actcggcgtc gccgaccggg tcgaattcat
     9121 tcacggggac gcaaccggct acgttgccga tgagaaggtc ggtgtggcgg cgtgcatcgg
     9181 cgccacatgg attggagggg gagtcgctgg caccatcaag cttctggcaa aaagtctgcg
     9241 ttccggagga atcatcctcg ttggggagcc ctactggctg cggctgccgt cgacggaaga
     9301 cacagccaag ggatgcggcg ccgattcgat tgccgacttc ctcacgcttc ctgaacttat
     9361 cgcatcgttc ggcgaacttg gctacgatgt ggtggaaatg gttctggccg accaggaaag
     9421 ttgggatcgt tatgaggccg ccaagtggct caccatgcgc cgctggctcg aaacgaatcc
     9481 cggcgacgac tttgcgaaag aggttcgagc gaaactgacc gcggagccaa aacgttacgt
     9541 cacctacacg cgggaatacc ttggctgggg cgtttttgcg ctgatggcgc ggtgacgtga
     9601 gcggcgatat ttctttccac aaaggattga ccacagggac cttgcaaagt aaaaaaaagc
     9661 gtgccagttg caagtaattg gcgatgaaac aatctgcgtt tttaatctca aataagtcgg
     9721 agcaacgaga cacggttaga actttttgtt agagaattgc aatattcatt gcctaacatt
     9781 tccaaaatct ttacaatatt agcttgtgga tgaaagctaa cattttttaa ggagattcgg
     9841 aaatggcgtt gaaatattta aatctggatg agaccacaag aaaattcatg cttcaagaag
     9901 ttgaccttga ttttaatagc gaaaaaggtc tcaatatgag taggaatttt aatgaagagg
     9961 gcatcagaaa ttggggggac tgtctaaaaa cagccattga aatgtatgat gatgcatggc
    10021 ttgcaaatca gcttcgtgat aataattata tgaaagagta tacagagagg cgaaaaccta
    10081 agggcggctt tactatggca aaagttccgg taactgctcc agagatgttc gcagaaggag
    10141 agtttaatag attttatgca cgtgggcttt gcttgcgagc aatagaagaa ggaattctca
    10201 acgtcatagt ctatcgtgca aaggatgtag ctcactcaag gcccgaatct gaagccaaga
    10261 taggtacttc tattgacgcc aacgtgcttt taaacgattt aagaacctca caaggcgagg
    10321 atactgagcg aggcattcca ggcggcccca attctggttt gtctatcaag cttccataat
    10381 tcttggggag taccggacgt tcccattggg tttgatggaa gcggttttta gcggagcggg
    10441 gcgggcagtc aaggcggggc ctcggcggaa aaggaagatt ttttgagcat tgttcaagct
    10501 gtagtttagc ctgggcaatg tgtcccgcag ggcaaaaaat actcttttcc gccggggtgc
    10561 ctttacggcc tgtcacgcgg agctatcttc catcatcaaa gccaatggga aatcgctacg
    10621 aattttcttt agggggcttt tcaataggcc ggggataaaa tataatccgg caatgggtag
    10681 gaaacgtaaa aaaatactgg gcaaaaatca tacctcgctg gcgatagacg aatgggcggt
    10741 atcaaggcgg attcatttta ttattgccat gatggcacaa attgtcgtgg cttctgcgga
    10801 aagcatgtat acagcctgcc gtgacgcgag ggtaaacgca aaatatggtg gtaatgttga
    10861 accgccttat ccgtctgatg accattggat gaatttttac aataacccgg acaaaccatt
    10921 agtaacggca atggaacttg ttattcccag taaagactgg tttacggttc ttttggggct
    10981 ggattctcaa caaatcgatg ctttaaatga gcagtttcat cgtattcagg aaaacagtga
    11041 taacttatcg ccccaagatt ctttttcaca tttgcttatg ctgtctaata attatgcaaa
    11101 catcatttat cggaatcata ttctcaaatt aagagatgca atagccggta ttgacgacgg
    11161 ctcaccagag cagataaaag ccgttaaggc ttcacctgag attctgttta tgattcgtgt
    11221 ttgggttcct tgttttctgg aatataaaat ttcgtttgac gaacttctga cgcaggctcg
    11281 cactggcgac attgaggcaa tagagaagat tattcggctt gacagaggaa ccgccagttg
    11341 tgacccgtta atacaaacac attttcttca agccaagcgt caaaatagaa aagcatgggt
    11401 tgaacgatat acaaaggcat tgaatgctga tgagccattg ggcgtgtttt cgtcgcagca
    11461 tatcaagtat ctgttggctg gttatattta tgagatgtcg gatattttct ccaaagctct
    11521 ggataaatgc tatcgctcga ttaaattgaa attcaaacgc ggctgtaagc cgaaatcgaa
    11581 attgagttat gaggatattt taagcctgtt tcattgctat gctgcggatt cgtatcccaa
    11641 aaatatcaag caactcccta tcattaaaga tgtcgatttt ctttcaattg acaacaagtc
    11701 ttttgaaact cgtgtccgtg aaacccgtaa gatttggcat gaaattctcg tttcacttat
    11761 tgcagaggtt aacgaagaag caacgaaatg ccaggctgaa tccgctgtgc aataaaacct
    11821 acaaagattt ttcggcaggt ataggggttt aaaccccgcc ataattttat tgtgcaagca
    11881 ttatgaatta tgcttgcaaa agtaaaatta tggtttacgc acaagggcgt atattaaagg
    11941 tcaaggttcc gcattcctcg caaacagcca atacgccggg gctgcgtggg aaaatcatcg
    12001 aattttccgc acagtcgcgt aagcggatga tagacctgct ggcccggatt gacacggaca
    12061 acgccgggtt cgtctgcttc atcacactga catatccaga ccttgacggc ccaccgtctc
    12121 atgaaattac cgaacgcgac cgccggacat ttttaaaacg catagcccgg cgttttcctg
    12181 aatcgtcggc aatctggcgg cgtgaacacg aacccagaaa gtccggcgaa caggcggggg
    12241 cgatttatcc gcattatcac atgctctttt tcggcatgcc gtttttcgac tgcgacgagc
    12301 tgaacgcaat ctggcgtgaa atcatgaatt atcccgatta cgtccgcacg gaaatcaagg
    12361 gcataaaatc gtggcggcag gcgatgtatt atctttcaaa atacatggcc aaaatcgcaa
    12421 ggcaaggcga cggaagccag ccggacggac aggcccccgc cggcgaggcg gggactgacg
    12481 acggcggcaa cgccgccggt tctcttgtct atggcacata tctgaccggt gataataaaa
    12541 taaataatga accgggaaag aaattgttgc ccgtaggccg tgcatgggga gtttttaacc
    12601 gccataattt accgctggcg gagctgcatt ccgcgtcgct tcctgcgggt cagtggatgg
    12661 aagcagtcaa ggacgctgcc gctcaagtct ggccgggcgt gaacgctgtc gacggttacg
    12721 ggtttacgct gtttctggat aatccgacaa ggtcaaaaca gtggttcgat ggaatatcca
    12781 agctcgccaa tgttttaccg ctaaaacctg acgacgggat tccgttttga ggctgattac
    12841 gatgcagaaa acagcacaac aaatcagcct cccgcccggc acgccagatt ggataacgct
    12901 cggcctcatc gccgaaacca tcgacacgtg gcagccttat tatcccaaat ccttgacacc
    12961 cgccgacgcg gtggagataa tccagagcat gggtaaattg atgtatacat tacagggtaa
    13021 gcattaaaag gcggttgggt gatgactaca aaaaattctt atttagccct tgctcgtgta
    13081 tcgtctcgcg aacaggaacg cgagggattt tctctggacg ttcaggaaga tgcgttgcgg
    13141 cggtatgcag agcgtcacgg cggggagatt gtgaagtttt accgtatcgc tgaaaccgca
    13201 agcaagcagg cggaacgcaa gacgttcaaa gagctgctcg aatacgccaa gaaaaacgct
    13261 cacaagctcg acggcctgct gttttacaag gttgataggg ctgccagaaa tcttttcgat
    13321 tacgtcgaac tcgaacgcct cgagatgaaa tacaacctgc ctatcgttta cgtagcccag
    13381 ccgaccgaaa acaccccggc aggacgcatg caacgccgca ttcttgcgaa catggcaagt
    13441 ttttatactg aacagcaaag cctcgatgtc cgcgaaggcc aatcccgccg ggctgaaagc
    13501 ggcctgttcc cctgcaaagc tccttacggt tatcgcaata atcgcgttga cggcagaagc
    13561 cttgttgaaa tcgacactga cgcagccgcc aatgtccgcc gcatttttga actgtattca
    13621 cagcacggcc aaacgctcga tggcgtggtt caggcgatgg ctgccgacgg cagaacttac
    13681 accagcacgc agccgcaatt tacacgcagc aaggttcacc atattctgcg tgaccgttcg
    13741 tatatcggcg aggttttgta tcgcgggcaa tggctggcgg ggaaacattc gccgcttatt
    13801 gggcgggaag ttttcgaccg cgttcaagtg ctgcttggcg acaagaccta tgcaagccat
    13861 gattcggttt acgggtcggg catggtcaag tgcggccatt gcgggcggcc tatggtttat
    13921 gaggtgaaaa ccaagtccac gtccagcggc ccgaaagaat accgctatta tcggtgcagc
    13981 cgttacactt cggcaggcca cccgcgagtg cgtatcaacg aaacgcagct tgataagcag
    14041 ataatcgaat ttttccggca gatgaaagta agtgacccca agattcatgc gtggatggtg
    14101 aaaattattc agctaaaagc cgagacgttc cagcgtgatg atgaaaagcg gctggatgat
    14161 ttgcaacggc agatggctgt ggcgaaccgt caacgcagca agctactaaa tatgcgtatt
    14221 gcagaggaaa ttgaccgcga tacattcgcc gctaaagatg cagaactccg cgaccagatg
    14281 cagcatttga gcgtgcttat cgacgcccac agccgggaga aatccgaata cgccgatatc
    14341 gccataaaag cgtttgaact ttcgcaacag cttgagaata agtggcttac cggcgatgtt
    14401 gccgcaaagc gtgaaatcct gaatattctc tgtttgaact acacgcttaa tgacgtaagt
    14461 cttgatatgg aaataagaaa gccctttgac gttctcgcca aag
//
LOCUS       DAONFI010000011        13843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_4827_length_13843_cov_4.440238, whole genome shotgun sequence.
ACCESSION   DAONFI010000011 DAONFI010000000
VERSION     DAONFI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_13843_cov_4.440238"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PLK08_00825"
     CDS             <1..1690
                     /locus_tag="PLK08_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HPS52071.1"
                     /translation="ATPKHNDGAIFAQTIADYPLVEAIKQNVVKHPVLPDIASRAKLH
                     ERQSAKYTEKYADYIDLGVIEWRKAYAEHEKMGKKAILFIMTDDTRNCDDVAEYLKGH
                     YPDFKDAVLVIHTKNNGEISEAATGKNKEELERLRKDANEIDAADSRYKVIISVMMLK
                     EGWDVKNVTTIVGLRAYSAQSNILPEQTLGRGLRKMYPGDIEEYVSVVGTDAFMDFVE
                     SIKAEGVILDHKPMGTGTGPKTPLVVEIDTENDKKDIDALDIEIPILSPRVYREYKSL
                     ADLDISAITHQRIAYMVFSEEELREIVFKDIANDKITHTTILDTTGIIDYRSVIGYFA
                     QSIMKELRLVSGYDILYGKIKAFVQDELFDRRVELENPNTMRNLSELAATKTLYETFK
                     KAINELTVRTKGDAEIRDTIKLRQTRPFVAKDQGYLVPKKSVFNRIIGDSDLELKFAD
                     FLERCSDVVSFAKNYLYINFKLDYANTTGNISNYYPDFIVRLSDKKVVIVETKGQEDL
                     DVPLKMERLKQWCEDVNRVQTDVKYDFVFVDEKSFHEFKPTSFRQLLEGFQEYK"
     gene            complement(1897..3576)
                     /locus_tag="PLK08_00830"
     CDS             complement(1897..3576)
                     /locus_tag="PLK08_00830"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPS52072.1"
                     /translation="MAKYALGLDYGTNSVRALLVDVSNGREIASSAWTYEHGKDGIIL
                     LREDPKFARQHPADYLKGAELTIKRTLGAAKRVVDNFSPNQIVGMGVDFTASTPLPVD
                     RRGVPLAFSERFEENPDSLAWLWKDHTADAEAEEITNMARRNRPQFLAKCGGAYSCEW
                     YFSKILHCLRHSPAVFNAAYTWVEAADWIPAVLTGTDTPEKIRIGVCQAGHKAMFNSE
                     WGGYPDEKFLKQIDPRIGELRGRLPAKAQDISKSVGGICAEWADKTGLPAGMPVATGS
                     IDAHMGAVGAGVEPGTMVQIIGTSACDMIVVPQDRALPDIPGVCGIVKDSILPGFTGI
                     EAGQSAVGDIFNWFVECIQPGGKKVGSHQALTKDAAQLKPGESGLLALDWNNGNRNVL
                     QDSRLSGLLVGQSLHTTPAEIYRTLVESAAFGLLTIITRLEQYNIRINNIVNCGGISE
                     KNAMIMQIYADVTGRPMKISRSSQTCALGCAIAGAVVGGAYETFPQAQAKMTGLKIRD
                     FEPKPEANQVYKQLFRLYRQLYDAFGTTAWKGNMGNIMKELIELRETVRKK"
     gene            complement(3666..7013)
                     /locus_tag="PLK08_00835"
     CDS             complement(3666..7013)
                     /locus_tag="PLK08_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52073.1"
                     /translation="MKRKLFLFFGTLIIILLAFGTYQMFVPGISIFDPTYHIRRPNFT
                     ASRGGSGPELGVVTEFSYTDYTADGRKRGTFTAKSWSQEDDGSFSLIKPSAVMYQKDG
                     TRTYMTADRGWIEGEQVAKGITVRRGRFEGNVVIYFDQSKDVNRKQPQDRTPEEILRD
                     CIRISMNDIEFSQELLELRTNSEIYLWSKTVDLVGKGLVILWNDSPRELRLLRIVDGD
                     IMLIKELPEQMDMIQLPTQSLAEQKRKENKQAEELKKATPKPKILVEKTSPTTAPVAP
                     ATQEGEGEFEITLGETVDSQGNTEPAQTMKIVRRSDSDARNIYEAQFNNNVRVFSGQR
                     KLIGAVLLTLKFEWDRAWRQGGGDKTSNTDSQALSDMNRDKTPSSAPADMAATKPAKG
                     KKLAIARPKGDNWMEIYWDGPLEIKPVGKTDSPNRKHCTLSGEGPAVQLSDADARIIC
                     RQFSFENPQQEANFRGAKDFPVRMMLARGDEIECNGLVRFVRSEGRAYLDGPGSMNRY
                     AKGGETGSGGEDYMLLPHLPTAASDLTEKITWQGNVIASFDEIKSGKSMRPFLKDAEF
                     RKNVFIERFMPDGKPSDYVRCDILDIDIAATSRGKAYPRQVTARGNVDAFQQGSKVLS
                     RFVQIDFVPRTDAAAANPQSMQIAGLDGARVEPSYIRARGDVDITYVDPKKTDEQPMR
                     LVGQTLDSDLLKRLAVVTGKPARIWQGTNKITGDVIFFDEPAEAVKVEGPGTLDFTSR
                     QDFNGNTSGRVRDVHIKWLRDMLYHGQSGLALINGNVDMLSEVDRNKGQDAMLCDQMK
                     LRFLKRQEDIASVVAANNRKDKKDKPLGLNIDRYSKRQISSIEALGSRNEMAALLTSS
                     QDPLNSGWLEKRMQLRGRRIVYNALTGEAEVKGAGTLMAEDYRLPEKKTLSGRTAGEI
                     PNPQSMDGVVDRPSQTGLMWSKGLQLDQRNRHVTVEGNVQMGHKSGNRINVAQARAQV
                     QPMDSLRSGRTSSLRADKLDVWFAEPKEGQVAGKTSDMLGREGPQLGPVKQFVADGDV
                     TFRDNNRMIDGQRVVYEYEDDGPEVLQRMLKVYGYLPGASQKKNARLTITDPISNTAQ
                     TVETPEITYDLLHDRLKTGTVSAGGGY"
     gene            complement(7041..7553)
                     /locus_tag="PLK08_00840"
     CDS             complement(7041..7553)
                     /locus_tag="PLK08_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPS52074.1"
                     /translation="MQKDLSKIKLLVVDIDGVLTDGKIIMTEAGDEIKNFNCKDGSGI
                     KYFNRAGGKVAIITGRDSALVLRRAGELGIESVRQNCKSKLPAYEEVLREHGVSQNEV
                     AVVGDDLPDLPMLLHCGFAACPADAVEDVASRVDYVCKLPGGAGCVREVIEMILRSSG
                     RWQEIMARYL"
     gene            complement(7563..8546)
                     /locus_tag="PLK08_00845"
     CDS             complement(7563..8546)
                     /locus_tag="PLK08_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPS52075.1"
                     /translation="MEKAYSFGRKVLELEGQAVLAAVRNVLAGDSFGRCIEAVLNCAG
                     QVVLTGVGKAGIIGQKISATLASTGTPSIFLHPTEALHGDLGRVGRNDVVMAMSNSGS
                     SEEIVRLLDHLKRRGVKIIGLTGKADSPLGRYADISLVYGEFAEACPLGAAPSVSTTV
                     LLAVGDAVSLTVMELREFGPEDYAQFHPGGALGRKFLKVAEVMTSRDGKCCCVSESLN
                     LRDALTEAENVGRRSGAILLVDTNGRLTGILTDADLRRRLLEKGAGILEKPVREIMTC
                     NPKHIAIDSLASEAEAVLNKYRIDELPVVDGDGCPVGVLDVQDVLGLNTLK"
     gene            8875..9528
                     /locus_tag="PLK08_00850"
     CDS             8875..9528
                     /locus_tag="PLK08_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPS52076.1"
                     /translation="MIFQVPTESLIFLLPALIMSLTVHEYAHARTAMHFGDNTAKSLG
                     RVTLNPLKHLDVLGTLALLLVGFGWAKPVPINPSYMRKRPFSEIMVSLAGPGSNLLLA
                     MAAAITLRIINAFDASGSLEALTTAIFYLAAINIILCVFNLMPLYPLDGHHILRDLLP
                     ASKRISYQYWQIRYGMIILLVIVFGPELLGKMTGRHVLDPLGWVLQKAIYLIYFIGE"
     gene            complement(9634..10038)
                     /locus_tag="PLK08_00855"
     CDS             complement(9634..10038)
                     /locus_tag="PLK08_00855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPS52077.1"
                     /translation="MSEKITKMELEIRVRYPEVDQMGYVHHSRYWQYFEMGRIEMLRR
                     QGYSYAEMERMGIFVVVVKAAIRYHLPAKFDEIIVVKTTIKKIGAARIDYEYEVRRKD
                     DNALICTAETTLASVDSSGQLTALPEFLREMA"
     gene            10167..11249
                     /locus_tag="PLK08_00860"
     CDS             10167..11249
                     /locus_tag="PLK08_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPS52078.1"
                     /translation="MTKMNISEIDPNFKSVRIGESDFDFYDVLESPVQITGVFWNKSE
                     NCFSRLPDEVLAAAGNELLVYLSRCTAGGQIRFTADSKRIALKFELFDNVDMLHMPRS
                     GSSGFDLFFEGEFIGKAWQSDFAKPQDQCIIADNQPRSQRREYTINMPLYNGVRRLYI
                     GLLPDSRLGGPISGPRKKICFYGSSITQGGCASKPGNAYTSMICRWQNLEQLNMGFSG
                     NAKGEPAIAQYIASQKPDVCVLDYDHNAPTLSHLRTTHEPFFKIIRKALPSMPIIIVS
                     RPNKNDGNEEYGSYRQRRDVIKTTHDNAAAGGDKKVFFVDGSCIMDGPDSSACTMDGI
                     HPNDLGFFRMACVIGEKVKKALEICN"
     gene            11519..>13843
                     /locus_tag="PLK08_00865"
     CDS             11519..>13843
                     /locus_tag="PLK08_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPS52079.1"
                     /translation="MLKEVLDNNLVVQITERLAGETPGKCVTLNGVWGSAQSLLAACI
                     GKKTGKPVMLLTHHLDDADAAADDIEIFLSTLSDNESGVHQFPAWEARLSGDRVSDEI
                     TGERLRLLDLLANPQTADEEVRFIVAPMMAMLQPVPTKEALAAGHLKLRCGMEMSIDS
                     LMAWLTDGGYERADQVDQQGQCAHRGGIVDIFSPGVQTALRVEFWGDTIESIRKFDLD
                     TQRSTETLQAFDVPSMAVGQCRAGDDFTATNFLEYLPDDTIIILPEPAELRDLANQLY
                     DRVRDDLVEESSPVALRKTSEVFAAISRFTRVEMHLFGGKSNTDAIRMGVRSLERLSI
                     NTGEALGELEEIAAVNNVWIFCENDAERQRLTETLNEHYPLLAKRVHFGQGHISTGFH
                     WPASNLAAVGHAEIYQRYSHIRRIRRVRAGRPIESMLDLNEGDYVVHVAHGIARFEGL
                     RKHERDDAGEEYLKLKFANGAVLNVPAAQINLVQKYIGAKGHRPTLSTLGGTQWAKAK
                     DRVTQAVDDLAAEMLRLHALRESMPGTVYPKDTPLQRQFAEEFVYTETEDQLSSIGQV
                     DADMSLPRPMDRLICGDVGYGKTEIAMRAAFKAVEAHRQVAVLVPTTVLADQHFRTFR
                     ERFADFPINVDVISRFRTAKQQNEIIKRLQLGQVDILIGTHRLLSRDVKFPDLGLVII
                     DEEQRFGVASKEHLKSLRTTVDVLTLSATPIPRTLHMALLGLRDISSLQTPPMDRRAI
                     HTEVCMFDETLIRTAINRELNRDGTVRRIIPPTLT"
BASE COUNT         3333 a         3763 c         3464 g         3283 t
ORIGIN      
        1 cgcgacacct aagcacaacg atggagcaat atttgcgcag accatcgcgg attatccgct
       61 ggttgaggct atcaagcaga atgtcgtcaa gcaccctgtt ttgcccgaca tcgccagccg
      121 tgcaaagttg cacgaaaggc agagtgcgaa gtatacagaa aaatacgccg actatattga
      181 tttgggtgtt atagagtggc gaaaagcata cgccgaacat gagaaaatgg gcaaaaaggc
      241 catcctgttt ataatgacgg atgatacccg taattgtgat gatgtggcgg agtacctgaa
      301 agggcattac ccagatttta aagatgcggt tttggtaatt cacacaaaaa acaatggcga
      361 aatttcagaa gccgctacag gcaaaaacaa ggaggaactt gagagactcc gcaaggatgc
      421 aaatgaaata gatgcagcag acagccgata caaagtcatc atttccgtaa tgatgcttaa
      481 agaaggctgg gacgtaaaaa atgtaacgac catcgtcggt ttgcgggcct attcggcaca
      541 gagcaatatt ttgccggaac aaacattagg tagaggtttg cgtaaaatgt atcccggcga
      601 tattgaagaa tatgtcagcg tggtaggcac ggacgcattt atggactttg tagaatccat
      661 taaggccgaa ggtgtaatac ttgaccataa acccatggga acaggaacag gacctaaaac
      721 gccgctggtt gtggaaatag atacagaaaa cgacaagaaa gacattgacg cgctggacat
      781 tgaaattcca atattatcgc caagagttta tcgagaatat aaaagcctgg ctgacttgga
      841 tatttcggcg ataacgcatc agaggattgc gtatatggtc ttcagtgagg aagaactccg
      901 ggaaatagtg ttcaaagata ttgcaaatga taaaattacg catacaacta ttcttgatac
      961 aacaggcatt atagattatc gtagcgtaat tggatatttt gcgcaaagca tcatgaagga
     1021 gttacgcctt gttagcggct atgatatttt gtacggcaaa ataaaagcat ttgtgcaaga
     1081 tgaattattt gaccgccgtg tggaactgga aaatccaaac acaatgcgta acctgtcaga
     1141 gttggcagcc accaagaccc tatatgaaac atttaagaaa gccatcaatg aattaacagt
     1201 tcgaacgaag ggcgatgcag aaatccggga caccatcaaa ctacgtcaaa cacgtccctt
     1261 tgtggcgaaa gaccaaggat atcttgtccc caagaaaagc gtttttaacc gtataattgg
     1321 cgatagcgat ttggaattaa aattcgccga ttttcttgaa agatgcagcg atgttgtctc
     1381 ttttgcaaag aattatctgt atataaattt caaacttgac tatgcgaata cgacaggcaa
     1441 tatttccaac tactacccgg attttattgt caggctgtca gacaaaaaag ttgtcattgt
     1501 tgaaacaaag gggcaggaag atttggatgt gccgcttaaa atggaaagac tcaagcaatg
     1561 gtgtgaagat gttaaccgtg tgcaaacaga tgtgaaatac gactttgttt tcgttgacga
     1621 aaagagtttt cacgaattca agccaacatc tttccggcaa ttgcttgaag gttttcaaga
     1681 atacaagtaa ggcttttacc gcaatctgga aaaatatcaa aaacgaaggt tttttgcagc
     1741 atttttggct tatctgtaca caaaatactc catgtcaaaa ggtttattga tttcggagat
     1801 gtcccccgct attacgctgg taaacagcgg acgatagaag ctgataacaa ctcgattaca
     1861 ggttgaatca gcttctattt tttgagaacc gtcgtattat tttttcctga ctgtttcgcg
     1921 aagctcgata agttccttca ttatattgcc catattgcct ttccacgccg tcgtgccgaa
     1981 ggcatcgtat aattggcgat ataatctgaa gagctgttta tagacctgat tcgcttcggg
     2041 tttaggttca aagtcccgaa ttttcagacc cgtcattttt gcctgagcct gcggaaaagt
     2101 ttcatacgct ccgccgacga ctgcgccggc aatagcacag ccaagtgcac acgtctgtga
     2161 cgaacgcgat attttcatcg gccggccggt tacgtcggca tatatctgca taatcatggc
     2221 gtttttttca gaaataccgc cgcagttgac tatgttgttt atcctgatgt tatactgttc
     2281 cagcctggtg attatcgtaa gcaggccgaa tgccgcagat tcaaccagag tgcgataaat
     2341 ctcagccggc gtcgtatgaa gcgattggcc cacaagaagg ccgctcagcc gcgaatcctg
     2401 caatacattt cgattgccgt tattccaatc caacgcaagc aggccgcttt cgccaggctt
     2461 gagttgtgcg gcgtctttag taagcgcctg gtgtgagcct accttcttgc cgccaggctg
     2521 aatgcactcg acgaaccagt taaaaatatc tccgaccgcg ctctggccag cctctatgcc
     2581 ggtaaaacca ggcagaatac tatccttgac tatcccgcat acgccgggaa tatccggcaa
     2641 cgcacggtct tgcggaacaa ctatcatgtc gcaagcgctc gtaccgatta tctgtaccat
     2701 cgtgccgggc tctacgcctg cgccaacagc acccatgtgc gcgtctattg agccggtagc
     2761 tacgggcatg ccagccggca gaccggtttt atccgcccac tcggcacaaa tcccgccgac
     2821 gcttttggaa atatcctgag ccttagcagg cagacgccca cgcagctctc ctatgcgggg
     2881 atctatctgt ttcagaaatt tctcatcggg ataaccgccc cactcggaat tgaacatggc
     2941 tttatggcca gcctggcaga cgccgatacg aattttttcc ggcgtatctg tgcccgtcag
     3001 gactgccgga atccagtcgg cggcttcaac ccacgtatat gccgcgttga agacggctgg
     3061 actgtgccgc aggcagtgaa gaattttgct gaaataccat tcacacgaat acgctccgcc
     3121 gcatttggcc aaaaactgcg gccggttacg acgagccatg ttggtaatct cttcagcctc
     3181 cgcgtcggca gtgtggtctt tccacagcca cgcaagcgaa tcgggatttt cttcaaaacg
     3241 ctcggaaaac gccagcggca cgccgcggcg gtcaaccggc agaggagtgc tggcggtaaa
     3301 atccacaccc atgccgacaa tctgattcgg cgaaaaatta tccactaccc gcttggctgc
     3361 gccgagggtg cgttttatgg taagttcagc acctttaaga taatcagccg gatgctgacg
     3421 cgcaaatttg gggtcttcac gtaaaagtat tatgccgtct ttgccgtgct catacgtcca
     3481 cgccgaagac gcaatctctc tgccgttgga aacatccacc aataacgcac gaaccgaatt
     3541 cgtgccataa tcaaggccta atgcgtattt cgccatatac acgtctcctt gccacgaaca
     3601 aacccactgg cacaaaaata ggaattctca actttgcaat gctctggcaa aagctgtttc
     3661 aaaactcaat atccgccgcc tgcgcttacc gtgccggtct tgaggcggtc atgcagaagg
     3721 tcataggtaa tttcaggcgt ttcgacagtt tgcgcggtat ttgatatcgg gtcagtaatg
     3781 gtcagccgcg catttttctt ctgcgatgcg cctggcaaat atccatacac tttcagcata
     3841 cgctgcaaca cttcagggcc gtcgtcctca tactcgtata cgactcgttg gccgtctatc
     3901 atgcggttgt tgtcgcggaa cgtaacatcg ccgtcggcta caaactgttt gaccggccca
     3961 agctgagggc cttccctgcc gagcatatca gacgttttgc cggcgacttg accttcctta
     4021 ggctctgcaa accaaacgtc gagcttgtcg gcccgcagcg acgaagttct gcctgaacgc
     4081 aacgagtcca tcggctgaac ctgcgcacgc gcctgggcca cgtttatgcg attgccgctc
     4141 ttgtgtccca tctgcacatt gccctcgacc gtaacatgtc tgtttctctg gtcgagctgg
     4201 aggcctttgc tccacatcag gcctgtctgg ctgggccggt cgaccacacc gtccatgctt
     4261 tgcggattcg gtatttcgcc ggcggttcgg ccggacaacg tcttcttctc cggcagccga
     4321 taatcctctg ccatcagcgt accggcgccc ttgacttcgg cttcgccggt cagcgcgttg
     4381 tacactatcc gacggcctct gagttgcatg cgtttctcca gccagccgga attaagcgga
     4441 tcctgggaac tcgtgagcag cgcggccatc tcgttcctgc tgcccaacgc ttcgatagat
     4501 gatatctgcc gcttggaata gcggtcgatg ttcaggccga gcggcttgtc ttttttatct
     4561 ttcctgttgt ttgcagccac gacggaagcg atatcttcct gccgcttgag gaagcgcagc
     4621 ttcatctggt cgcagagcat ggcgtcctgg cctttgttgc ggtcgacttc gctgagcatg
     4681 tccacgttgc cgtttatcag cgcaaggccg ctctggccgt gatagagcat atctcgcaac
     4741 cattttatgt gtacgtcgcg cacgcggccg gatgtattgc cattgaaatc ctgtctgctg
     4801 gtgaaatcaa gcgtgccggg gccttcaacc ttgacggctt cggcgggttc gtcgaagaat
     4861 atcacgtcgc ctgttatctt gtttgtaccc tgccagattc gggcaggctt gccggtaacc
     4921 actgcaagcc gcttgagcag gtcgctgtcg agtgtctggc cgaccagccg catcggctgt
     4981 tcatccgtct ttttggggtc gacatacgtt atgtcaacgt caccccgtgc gcggatgtag
     5041 ctcggctcga cccgtgcgcc gtcaaggccg gcaatctgca tgctttgcgg attggcggca
     5101 gcggcatcgg tgcgaggcac gaaatcaatc tgcacgaatc gcgacaaaac cttgctgccc
     5161 tgctgaaacg catccacatt gccgcgggct gtaacctgcc tgggatacgc cttgccccgc
     5221 gacgtagcag cgatgtctat atcgagaata tcgcaacgca cgtaatcgct cggcttgccg
     5281 tcaggcatga accgttcgat aaaaacgttt ttgcggaact cggcatcctt caggaacggc
     5341 cgcatacttt tgccgctttt tatctcgtcg aacgacgcaa tcacattacc ctgccacgtg
     5401 attttttccg tcaggtcgct ggcagccgtc ggcagatgcg gcagaagcat gtaatcttcg
     5461 ccgcctgagc cggtctcgcc gcctttggca tagcggttca tcgagcccgg cccgtcgaga
     5521 tatgccctgc cttcgctgcg aacaaaccgc acaaggccgt tacattcaat ttcatcgccg
     5581 cgagcgagca tcattctgac tggaaaatcc tttgcgccgc ggaaattggc ttcctgttgc
     5641 ggattttcaa agctgaactg gcggcatatt atacgggcgt cggcgtcgga aagctgaact
     5701 gccggccctt cgcctgaaag cgtgcaatgt ttgcgattgg gcgaatcggt tttgccgacg
     5761 ggcttgattt caagcgggcc gtcccagtaa atttccatcc agttatcgcc cttgggcctt
     5821 gcgatggcga gttttttacc cttagcaggc ttggtcgcag ccatgtctgc cggcgcagaa
     5881 ctcggagtct tgtccctgtt catatcgctg agcgcctggc tgtcagtgtt ggacgttttg
     5941 tcgccgccgc cctggcgcca cgccctgtcc cattcaaact tcagcgtaag gaggacggcg
     6001 cctatcagct tgcgttggcc ggaaaacacg cggacgttat tgttgaattg agcttcgtaa
     6061 atgttacggg cgtcactgtc gctgcggcgg acaattttca tggtctgcgc cggctcggtg
     6121 ttgccttgcg aatccacagt ctcgccgagc gttatttcaa attcgccctc gccttcctgc
     6181 gtcgcgggtg caacaggagc agtagtgggc gaggtttttt ccaccagaat ctttggtttc
     6241 ggggttgctt ttttgagttc ttcagcctgc ttgttttcct tgcgcttctg ttcagccagc
     6301 gactgcgtgg gcagctgtat catgtccatc tgttcgggca attccttgat gagcattatg
     6361 tcaccgtcga cgattcgcag cagtcgcagt tcacgcggac tgtcgttcca gagaatcacc
     6421 aggcccttgc cgacgaggtc gaccgttttc gaccacaggt atatttcgct attggttcgc
     6481 agctcaagga gttcctggct gaactcgata tcattcatgc taatgcgtat gcagtcccgc
     6541 agaatttctt caggcgtacg gtcctggggc tgtttgcggt ttacgtcttt cgactggtca
     6601 aaatatatta cgacattgcc ttcgaatcgg ccccgcctaa cggttatacc cttggcgacc
     6661 tgctcgcctt caatccagcc gcggtcggca gtcatgtaag tacgagtgcc gtctttctga
     6721 tacattacgg cagacggttt tatgaggctg aaactgccgt cgtcctcctg cgaccagctt
     6781 ttggcggtaa aagtgccgcg ttttctgccg tcggcagtgt aatcggtata gctgaattcc
     6841 gtcacgactc ccagctcagg cccgctgccg ccccgggagg cagtgaaatt aggccgacgg
     6901 atatgatacg tcgggtcgaa tatcgatatg ccaggcacga acatctggta tgtgccgaac
     6961 gccagcaata tgattatcaa tgtcccgaaa aacagaaata acttacgttt catttgaaat
     7021 tcctaatagc agtcatcgtt ttacaggtag cgggccatta tctcctgcca ccgacctgaa
     7081 ctgcggagaa tcatttctat gacctcccgc acgcagcctg ctccgcccgg cagtttgcaa
     7141 acataatcga cgcggctggc gacgtcttcg accgcgtccg ccggacatgc cgcgaacccg
     7201 caatgaagca gcatcggcaa atcgggcaga tcgtcgccta ctacggcaac ttcattctgc
     7261 gaaacgccgt gttcgcgcag cacttcctca tacgccggca gtttgctctt gcagttctgc
     7321 cgcacgctct ctatgccaag ttcgccggca cgacgcagaa ccagcgcact gtcacggccg
     7381 gtaatgatgg cgaccttgcc gccggcacga ttgaaatatt ttatgccgct gccgtccttg
     7441 cagttgaaat ttttgatttc gtcgccggct tcggtcatga tgattttacc gtcggtcagt
     7501 acgccgtcga tatcaaccac cagcagcttt attttcgaca aatccttctg catgtgtgat
     7561 tcctatttga gcgtattaag gcccagaacg tcctggacgt ccagcacgcc tacgggacag
     7621 ccgtccccgt ccaccacggg caattcgtcg atacgatatt tgttcagaac ggcttcggct
     7681 tcgctggcca gcgagtctat ggcgatgtgt ttgggattac aggtcataat ttcacggacc
     7741 ggcttttcga gaatgccagc ccccttctcc agcagccgtc ttcgaaggtc tgcgtcggta
     7801 agtatgcctg tgagtctgcc gttcgtgtcc accagcagaa tcgcgccgct tcgacggccg
     7861 acgttttccg cctctgtcag tgcgtcgcgc aaattgagcg actcgctcac acagcagcat
     7921 ttgccgtcgc gggaggtcat gacctcggct accttcagaa attttctgcc cagcgcgccg
     7981 cccggatgga attgcgcgta atcttcaggg ccgaattccc gaagctccat caccgtaagc
     8041 gacaccgcat cgccgacggc taataatacc gtggtcgaaa cgctcggagc ggcgcccagg
     8101 gggcatgctt cggcaaattc gccgtaaacc agtgaaatgt cggcataacg gcccagcggc
     8161 gagtcggctt tgccggtaag gccgattatc ttcacgcccc gccgtttgag gtgatccagc
     8221 agccggacta tctcttcact gctgccgctg ttggacatcg ccataaccac atcgttcctg
     8281 cccaccctgc ccaggtcgcc gtgaagcgcc tcggtcggat gaagaaaaat gctcggcgtg
     8341 ccggtagaag ccaacgtagc cgatattttc tgaccgataa tgccggcttt gcccacgccg
     8401 gtcaatacta cctggccggc acagttcagt accgcctcga tacagcggcc aaaactgtcg
     8461 ccagccaaaa cattccgtac agccgccaga accgcctggc cttcaagctc aagtactttg
     8521 cggccaaacg aatatgcctt ttccatgtct gtcacaaaaa atcctttcac ggtatcttat
     8581 atatacacca tgcatgcgca gggaatacct atagtgaagc gatattatat caatacccct
     8641 ctcacattca cagccttttt aaccggatac cgccccttaa aaaaagatga atttaacaaa
     8701 aattggctac tttcgaaacg caggcaggaa aacccgcccg acgcatttgc tgatattctg
     8761 ccgttttccg caccggcggg agaaaaaatc tcagcctcgc cataaccagc agcttttgaa
     8821 ggaacatcca gttttcactt gagaaaaaac cggctgtccg ttaaaaggta tgccatgata
     8881 tttcaagtcc caaccgaatc actgatattt ctgctgccgg ccctgattat gagcctgaca
     8941 gttcatgaat acgctcatgc ccgcacggcg atgcatttcg gcgacaacac cgccaaaagc
     9001 cttggccgcg taacgctcaa cccgttaaag catctggatg ttctgggaac gctggcgtta
     9061 ctgctggtgg gcttcggctg ggctaagcct gtgccgataa atccgtcata catgcgtaaa
     9121 cgccctttca gtgaaataat ggtctcgctg gctgggcccg ggagcaatct gctgctggcg
     9181 atggcagccg cgataacgct gagaattata aacgctttcg acgcatccgg cagccttgag
     9241 gcattgacga cggcgatttt ctatctcgca gctatcaaca tcatactttg cgtattcaat
     9301 ttaatgccgc tttacccgct ggacggccat catatcctgc gcgacctgct gccggcgtcg
     9361 aagagaatat cgtatcaata ctggcagata cgatacggca tgataatatt gctggtaatc
     9421 gttttcgggc cggaacttct gggcaaaatg accggccgac acgtcctgga cccgctgggt
     9481 tgggtactcc aaaaagccat atacctgatt tacttcatcg gcgaataatt ttttaaaaac
     9541 accgtctacg aattaccttt tcaatttttt ttaaaaaaac cactgataac aaatagactt
     9601 ttcaaaatat tttttaaaaa acaccgtcca cgtctacgcc atttcgcgta aaaattccgg
     9661 caatgccgta agctggccac tgctgtccac gcttgctagc gtcgtctcgg cggtacaaat
     9721 cagcgcattg tcatctttgc gtctgacctc gtattcgtaa tcgatgcggg ctgcgccgat
     9781 tttttttatc gtggttttga cgactatgat ttcgtcgaat ttcgcgggca gatgatatcg
     9841 aatggctgcc ttgaccacga ccacgaatat gcccatgcgt tccatctcgg cgtatgaata
     9901 gccctgccgg cgaagcatct ctatgcggcc catctcgaaa tactgccagt agcggctgtg
     9961 atgaacgtaa cccatctgat ccacttccgg ataacggacg cgaatctcca gttccatttt
    10021 tgttattttt tcgctcatga acggaattta ccctaactga agcaattttg caatacgccg
    10081 tcattgacat ccggcatcat aaaaaatata accaaattaa attcggaagt taatccagta
    10141 tctgcaaaat caggattttc ccaaaaatga caaaaatgaa tatttcggaa atagacccta
    10201 atttcaaatc agtccgcatc ggcgaaagtg atttcgattt ttatgatgtt ctggaatcgc
    10261 ctgttcagat tacaggcgta ttctggaaca aatccgaaaa ctgtttttcg cgtcttccgg
    10321 atgaagtact cgccgcagcc ggcaatgagt tgctggttta tctcagccgc tgtactgcgg
    10381 gcggccagat acggttcacc gccgactcaa aaagaatcgc cctgaaattc gagcttttcg
    10441 ataatgtcga catgcttcat atgcctcgca gcggttcaag cggtttcgac ctgtttttcg
    10501 agggcgaatt catcggcaag gcatggcaga gcgacttcgc aaagccgcag gatcaatgca
    10561 taatagccga caaccaaccc cgcagccagc gccgcgaata taccataaac atgccgttgt
    10621 acaacggcgt aagacgtctg tacataggcc ttcttcccga cagcagattg ggcgggccaa
    10681 tcagcgggcc gcggaagaaa atctgttttt acggctcttc cataacgcag ggcggctgcg
    10741 cgtccaaacc aggcaatgca tacacgagca tgatttgcag atggcagaac ctcgaacagc
    10801 tcaatatggg tttttccggc aatgcgaagg gcgagccggc gatagcgcaa tacattgcct
    10861 cgcaaaaacc cgacgtctgc gtactggatt acgaccataa tgcaccgacg ctatcgcacc
    10921 ttcggactac gcatgaaccg tttttcaaaa taatccgcaa agctctgccg tcgatgccga
    10981 taatcatcgt aagcaggccg aataaaaatg acggcaatga ggaatacggc tcatacaggc
    11041 aacggcggga cgtcatcaaa accacgcacg ataacgcagc cgcaggtggc gataaaaagg
    11101 tatttttcgt ggacggctcg tgcatcatgg acggccctga ttcatcggcg tgcacgatgg
    11161 acggcataca tccgaatgac ctgggctttt tcagaatggc ctgcgtaata ggcgaaaaag
    11221 tcaaaaaagc actcgaaatc tgtaattgac atctttttgg gaaacgattt tttatccgat
    11281 ttttatccgg tttattcaaa aacgtaaaaa tcaacggctt aaagtaaggc ggcaatacgg
    11341 aaatttagag catgttaaac ttgagatgcg acactttatg cgttggcgaa acgagctatg
    11401 caaggaaggc gaagcaggaa tactgcccgt attccgatgc agcctgacgt agcagagcga
    11461 tgttgcagcc agcagaaatg acgcatctca agtttaacat gctctagttc aaaagaaaat
    11521 gctcaaagag gtactggaca acaatttagt agttcaaata accgaacgcc tggctggtga
    11581 aacacctggc aaatgcgtta cgctcaacgg cgtatggggt tctgcgcagt cgctgctggc
    11641 ggcatgcatc ggcaaaaaga caggcaaacc ggtaatgctt ctgacgcacc atctcgacga
    11701 cgccgacgcg gcagccgacg atatcgagat tttcctgtca accctgtcag acaatgaatc
    11761 cggcgtccat caattcccag cctgggaagc cagactatcc ggcgacaggg tcagcgacga
    11821 aataaccggc gaacggctgc ggctgctgga tttgttggct aacccgcaaa ctgccgacga
    11881 ggaagtgcgg ttcatcgtcg caccgatgat ggcgatgctt cagccggtgc cgaccaaaga
    11941 agctctcgcc gccggacatc tgaaattgcg atgcggcatg gagatgtcca tcgactctct
    12001 catggcctgg ctgaccgacg gcggttatga acgcgcagac caggtcgacc agcaaggtca
    12061 atgcgcacat cggggcggca tcgtcgacat attttcgcca ggcgtacaga cggcacttcg
    12121 cgtggaattc tggggcgaca caatagagtc aatccgcaaa ttcgatctcg acacgcaacg
    12181 aagcaccgaa acactgcagg cgttcgacgt tcccagcatg gctgtgggcc agtgtcgtgc
    12241 cggcgacgat tttaccgcga cgaattttct ggaatatctg ccggatgata caatcataat
    12301 tcttcccgaa ccggcggaac tgcgagacct ggccaatcag ctttacgacc gtgtgcgtga
    12361 cgacctcgtg gaggaatcgt cgcccgtggc gttgcgaaag acgtctgaag tttttgccgc
    12421 gataagccgg ttcactcgcg tcgaaatgca cctgttcggc ggcaaaagca ataccgacgc
    12481 aattcgtatg ggtgtgcgtt cgctggagcg gctgagcata aacacaggcg aagcgctggg
    12541 cgaacttgag gaaatagccg ccgtcaacaa cgtgtggata ttctgcgaaa acgatgcgga
    12601 acgtcagcgg ctgacagaaa cgctgaatga acattatccg cttcttgcca aacgtgtaca
    12661 tttcgggcag ggccatattt ccacgggttt tcactggcct gcctccaacc ttgccgcggt
    12721 cggccatgcc gagatttacc agagatattc gcatatacgg cgtatccgca gggtgcgagc
    12781 cggccggcct atcgagtcga tgctcgacct taacgaaggc gattacgtag tacacgtggc
    12841 gcacggcatc gccagattcg aaggcctccg aaaacacgaa cgcgacgatg ccggcgaaga
    12901 atacctcaaa ctgaaattcg ccaacggcgc cgtactgaac gttccagccg cccagattaa
    12961 tctggtgcag aaatatatcg gcgccaaagg ccatcggccg acgttaagca cgctcggcgg
    13021 cactcaatgg gccaaggcta aagaccgcgt aacgcaggcg gtcgacgacc tggccgccga
    13081 aatgctccgg ctgcacgccc tgcgggaatc catgcccggc acggtctacc cgaaagatac
    13141 gccgctgcaa cggcaattcg ccgaagaatt cgtctatacc gagactgaag accagctttc
    13201 atcaatcggc caggtggatg cggacatgtc gcttccgcgg cccatggaca ggctgatatg
    13261 cggcgacgtc ggatacggca aaacggaaat cgccatgcgc gccgctttca aagcagtcga
    13321 agcacacagg caggtcgccg tactcgtgcc aaccactgtg cttgccgacc agcatttccg
    13381 cacattccgc gagcgtttcg cagacttccc catcaacgtg gatgtcatca gccggttcag
    13441 aaccgccaaa cagcagaacg aaatcatcaa acgtctgcaa ctcggccagg tggacatact
    13501 catcggcact cacagactgc tgagcaggga tgtcaaattc cccgacctcg gccttgtcat
    13561 tatcgacgag gaacagcgtt tcggcgtagc ctctaaggag catctcaaat cgctccgcac
    13621 gacggtggac gttctgacac tctcggcaac gcccatcccg cggacgctgc acatggccct
    13681 gctaggcttg cgggatattt cgtcgcttca gactccgccc atggatcgcc gggccattca
    13741 caccgaagtc tgcatgttcg acgaaacgct tatcaggaca gccatcaatc gcgagctcaa
    13801 ccgcgacggc accgtccgcc gcatcatccc gcccaccctc acc
//
LOCUS       DAONFI010000012        13627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_4955_length_13627_cov_4.449971, whole genome shotgun sequence.
ACCESSION   DAONFI010000012 DAONFI010000000
VERSION     DAONFI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13627
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_13627_cov_4.449971"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLK08_00870"
     CDS             <1..248
                     /locus_tag="PLK08_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPS52080.1"
                     /translation="AEATDRNMLDWPKVYQEILKLKPSDQNLLVLRFFENCSTARIAE
                     ITSMAHGTVRTRLSRIITKLKKKFQDSEVAEYETKQH"
     gene            229..660
                     /locus_tag="PLK08_00875"
     CDS             229..660
                     /locus_tag="PLK08_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52081.1"
                     /translation="MKQNSIKNLLNSLPFDDTINQTHKAKLKDAVLHVHAVACKRRES
                     RWRNAAVIIFAIGMGLAVSTQIWRETKKHDYVTIQKTLPAVASRPTLNEQEKPPQAVN
                     PSIWSKKAWKKRFDDIQLSQNSQQRIIWISPTRKPILGEQR"
     gene            657..2486
                     /locus_tag="PLK08_00880"
     CDS             657..2486
                     /locus_tag="PLK08_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52082.1"
                     /translation="MKNLKKMKLFTLLAIATFLLAGCYPQAAVIWSHDGQHGAVLTKN
                     EIYLCDSQGKLSTESLKYSGAVAWSPNSDDLYYVQPLMINSWKEAAKYVDAKKQAELI
                     ALAKDALAKLQPESLKDDKNNPFAKFEPEDTILMLLYLKDNQADELKTKLDAKTLQGI
                     AKAKAQLNQLFVCKNCNTAQPQLILSTFMNIENLKISPNGKAVEFSKSGKTLYVCDMK
                     GRVSLIAQNAAILPDWTSDGNTLIYSSTNNGGIQPPRLTYTQDKKSWRINTSDEIYDV
                     SGGNMSYEYKNGKFSAIGHNVTIISSTSGNKLLAETFIYVYDQNNSAASSTPSDKLQL
                     GSIKKVKIFDERGEMFKKFPDATELAGIVFHDTCKIRCLKNGWIIFSSVPLSVPATTQ
                     EMPQSQSLFAITPELPSTIINIIPKETQRQMGSINWDTFQMSPDQTRIAVLSNDKSVK
                     ILNLKTSNIQIVQPPLAGLDAPVLPTWQNNENLCILTCKESADEKVYMVDLWSNGSRK
                     TISTGWPKIIMQGIVDKYRSNKSAAPVKAAIKSAETEIHMNMKSCAKATTQPTAKKCP
                     AKVIHMKSYIKPANPTTQPAEKNHTTEIHMSGSYISTNQLAVD"
     gene            2684..3199
                     /locus_tag="PLK08_00885"
     CDS             2684..3199
                     /locus_tag="PLK08_00885"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HPS52083.1"
                     /translation="MLYEPTGDRTELDHSRQPAKALGKTGSGNSQRGDKRYFTPEHAN
                     RALVLVRRIVSEIVTGYARLLEIEEMIESAERTGSNDGLEQARKRLVKTVDDLQTCLD
                     ELDQIGVELRDFSRGIVDFPSIHEDREIKLCWLLGEESVCHWHEAGAGFACRQPLCLL
                     GKNEILQPTLF"
     gene            3586..4485
                     /locus_tag="PLK08_00890"
     CDS             3586..4485
                     /locus_tag="PLK08_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52084.1"
                     /translation="MLAVLLFSVSPAAAISDDENLQKVYAALELGQADHDVLAKLSDG
                     TENIHENEFFWMMKKISALPSLSQDELNQLDAPAWKTLISAPQRFRFLPVRMKVCVYT
                     LQKLSVENRHLIRSPYWPSDRPIYRIHAVKMKGNEPNPNEPLVIFSQTLPPNLPEKSE
                     LQADGQTAYKTGPLYEIAGVFYKYIETGNSTGTFVNKYPVILAWQMNPAKIQAPQGAL
                     SRFAFGIVILIIALAIAVFMFLKRRIRSQKKGFGYETLFSHDKSRTESEEDIDPMSAI
                     PNELADAAREYRLEHPDEPADAK"
     gene            4501..5436
                     /locus_tag="PLK08_00895"
     CDS             4501..5436
                     /locus_tag="PLK08_00895"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPS52085.1"
                     /translation="MTCKNDLSVDIAGVNLQNPLMTASGTCGYADEYGDFVNLRELGA
                     FVTKSISLAPRKGNDYPRIVETRAGMLNAIGLANVGLDTFVNEKVPQLEALGLPVFVN
                     IAGTTPDDYVKVAAALENVDIVKGVELNISCPNVKAGGIQFGVQPELVEEITRTIKSV
                     CRKNILIVKLSPNVTDITATAQAAVDGGADALSMINTFTAMVVDIEKRRPVLANRTGG
                     LSGPAIRPIAVYMVHRVYTQVAKPAGIPIIGMGGIQYARDAIEFLLAGATGLAVGTAL
                     FVDPRCILEIKQGIDEYLTRHKKGSIRDIIGTLEG"
     gene            5704..7167
                     /locus_tag="PLK08_00900"
     CDS             5704..7167
                     /locus_tag="PLK08_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS52086.1"
                     /translation="MRVFMLGWEFPPFISGGLGTACYGLTKGLNEIDVDICFVLPTAI
                     PIDATTTGKTHVQLRSPSDVEPARLQRLMNPLSEEEEETTLEVTHNRRTLHTRKHHHL
                     RVEHLPAFLMPYATPETYEKNIVELRRYARNSAYKQALRYAIAKANGNHHCLENWHFP
                     LETSAVDSDDANKSIIQGTAGAHYEGDLFAQVHRFASLAVELAMREDFDIVHAHDWMT
                     YPAGIAVAQATGKPLVVHVHSTEFDRSGQNVNQVIYDIERAGMTLAKSVVCVSHLTAN
                     IAKHRYGVPENKITVIYNAVELPARPNGPLKTIAKNEKIVLFLGRVTMQKGPEYFLQA
                     AKRVVEKYKKVRFIVAGSGDMITRCIKMAADLGIGKYVTFTGFLRGQDVDRVFRMADL
                     YVMPSVSEPFGIAPLEALSRDVPVIISKQSGVSEILTHVLKVDFWDTDEMANKILAVL
                     RHEPLGKTLQQHSKIELHGISWHDSALKLKELYATLV"
     gene            7283..8476
                     /locus_tag="PLK08_00905"
     CDS             7283..8476
                     /locus_tag="PLK08_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPS52087.1"
                     /translation="MASVVFYFQVHQPYRLRRYSIFDTSENYFDDYSNEQILRKVAEK
                     CYLPTNRLMLDLIRKHQGRFRICYSITGTILEQSQQWMPEVVDSFKALADTGCVEFIA
                     ETYFHSLAFLYDRDEFRRQAKMHCQLMQDLFGYSPKVFRNTELTYNNQVGKLVEDMGF
                     NAILTEGADFVLGYRSPNFIYHPPACKKLKLMMKNYRLSDDIAFRFGNRAWAEWPLTT
                     EKFAGWVDQINGCGYTCNLFMDYETFGEHQWADTGIFDFMYHLPDAIFAGGKNDFLTV
                     SQAIARYPDVGEVDVPHMISWADTERDLSAWLGNSMQSNALTDVYELASQVLATKNKR
                     LIHDWQKLQTSDHFYYMCTKYFSDGDVHKYFNPYESPYDAYINYMNILDNVRARIAQC
                     QPATV"
     gene            8573..9088
                     /locus_tag="PLK08_00910"
     CDS             8573..9088
                     /locus_tag="PLK08_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52088.1"
                     /translation="MNVNTMKNLATFFCGMAALIAGCDGISPKGGNVETCEKVPDPIS
                     LLLPQKISIHPFTQTATFEDGKQGLHMRIQFTDFFGDPTKAFGDLRFELYEFKPYQQH
                     KRGDQLDIWDEYISDPDKNMIHWDSHTRSYEFRLASKNPILPGQKFILVVYFNSRFTP
                     RMTAETVLTAE"
     gene            9236..10054
                     /locus_tag="PLK08_00915"
     CDS             9236..10054
                     /locus_tag="PLK08_00915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPS52089.1"
                     /translation="MSDNSPRPLALITGASSGIGAEFARSLAKRGCDLILVARREDKL
                     RKLADDIHQDCDAAIEIFPADLADDNQRHAVADRIRQTGGLEYLVNNAGFGTKERFWQ
                     ADFASQDIMARLHVLAVMELCHAALGDMVKKGGGNIINTASVAGFFQSPSNVVYCSTK
                     AWIISFTEGLAFELVGSGVNVQALCPGYTLSEFHDVMQISRDGIPKSWWLPANVVVHD
                     SLAALARRKLVCVPGLWRYKLLIAISPFLPRWLKYRVAAARHKRVIKTGKPIMM"
     gene            10202..10372
                     /locus_tag="PLK08_00920"
     CDS             10202..10372
                     /locus_tag="PLK08_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPS52090.1"
                     /translation="MAHVISDDCTKCGACAAVCPVEAISEGETKYIIDPDTCVDCGAC
                     AGECPVSAISAQ"
     gene            10445..11014
                     /locus_tag="PLK08_00925"
     CDS             10445..11014
                     /locus_tag="PLK08_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPS52091.1"
                     /translation="MSIYALLGIAVGLAMDAFAVAIAAGLTLPKVTLRHSLRLAWHFG
                     VFQAIMPIIGYFAGKNFAGNLFYKYDHWIAFSLLTLLGGKMLYEAFTRNADSEKSDPT
                     KGWRLVTLSVATSLDALAVGVTMALMKTEIFTPALIIGIVAFIFTILGIRGGSWLGEK
                     WEHISEFIGGLVLILIGVKILADSLGWLG"
     gene            complement(11018..11965)
                     /locus_tag="PLK08_00930"
     CDS             complement(11018..11965)
                     /locus_tag="PLK08_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HPS52092.1"
                     /translation="MSQIRLEYAGPRGKSRFAKQLGLSPSTYDYYEASRVPPVDVLIR
                     ISEVAGVDLGWLLTGRKSADGLDVNNPIVQRAARLLREQPSSARPLAAFLEILEGIKA
                     FPPKENSVDDVGQGNLAAENVAFGAVGKVASAGGGRRGWIPILGRSAAGISHFWADDE
                     QVADIVTLGDLIEKHVGEVRDEKIAAVSRASSGDVQQGVQIITLDRPDVPGGAVEFIA
                     SEAICRKYPDAFALRIDGDSMSPEIFHGDIVVLSPSVRAVVGEPAVIQLAGAIGVTCK
                     IYHPADDGLHMVAANADYKPTIVPMASVNWALKVLAKIR"
     gene            12158..12451
                     /locus_tag="PLK08_00935"
     CDS             12158..12451
                     /locus_tag="PLK08_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52093.1"
                     /translation="MKKSSTNIGRRKGAKTHKFDDKSSSNSAEHNASANNLSAPHAAR
                     KGITSAKKLPGHPVRKVDARSLHRILTWSIELSQRQQQLMDAAIEILANAKND"
     gene            complement(12558..13295)
                     /locus_tag="PLK08_00940"
     CDS             complement(12558..13295)
                     /locus_tag="PLK08_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS52094.1"
                     /translation="MQAGTLNPISVTGWNQDVVIGVGETYNTGSTAQLDNASGTTGGA
                     MVWYGVGLNTSYPTTGLPTGLIQSATSSDVYFQLQSFGSAAGTNNNAVYGGGTLTLVT
                     VAAYDHIALIGATGQGTANLTITFNYTTGDPDVYSSLGTDSINHDWFGGGSIAYTANG
                     RISTVSGDFEYVNSGNPRLYETIFDVDETRLLQSIVIVDNAVPSDPDVPQHNIIMAVS
                     GDTVPEPATLVLLVAGMGAVFAVRRRK"
BASE COUNT         3847 a         3482 c         3231 g         3067 t
ORIGIN      
        1 ccgcagaagc cacagaccga aacatgctgg attggccgaa agtgtaccag gaaattttaa
       61 aactcaaacc gtccgaccag aatctgctgg ttctgcgttt ttttgaaaac tgctccacgg
      121 caaggattgc cgaaataacc agcatggcac atggcacggt tcgcacgcgg ctgagccgaa
      181 taatcacgaa attgaaaaag aaattccaag acagtgaggt ggctgaatat gaaacaaaac
      241 agcattaaaa atctgctcaa cagccttccg tttgacgata cgataaacca gacccataag
      301 gcaaaactga aagacgccgt tctgcacgta cacgccgtag cctgtaaacg cagggaaagt
      361 cggtggcgaa acgcagccgt aataattttc gccatcggca tgggcctcgc ggtaagcact
      421 caaatctggc gagaaacaaa aaaacacgat tacgtgacaa tacaaaaaac actgccagcc
      481 gtcgcaagcc ggccgacact aaatgaacag gaaaaaccgc cgcaggctgt aaatccgtcg
      541 atatggtcga aaaaagcatg gaaaaaacgt tttgacgaca tacagctgtc acaaaattca
      601 caacaaagaa taatatggat aagtcctaca cgaaaaccaa tattgggaga acaacgatga
      661 aaaaccttaa aaaaatgaaa ctttttacat tactcgcgat tgcaacgttt ctgttggccg
      721 gctgttatcc gcaggcagcc gtcatatggt cgcacgacgg ccaacatggc gcggtactca
      781 caaaaaatga aatctatctc tgcgactcac aaggcaaatt atcgacagaa tctctgaaat
      841 attcaggcgc agttgcatgg agtccgaatt ccgatgattt atattacgtt cagccgctta
      901 tgataaattc gtggaaagag gcggctaaat atgtagatgc aaaaaaacag gcggaactga
      961 tagcactggc caaagacgcg ctggccaaat tacagcctga gagtttaaaa gatgataaaa
     1021 acaacccctt tgcaaaattc gaaccggaag acaccatact aatgctgttg tacctgaaag
     1081 acaatcaggc tgacgaactc aaaaccaaac tcgatgcaaa aacattgcag ggcatcgcaa
     1141 aagcaaaggc acagttgaat caattattcg tctgcaaaaa ctgcaacacg gctcagccgc
     1201 aattaatcct cagcactttc atgaatatcg aaaacctgaa aatctcgcct aacggcaaag
     1261 cggtcgaatt ttcaaaatcc ggaaaaacgc tctacgtatg cgacatgaaa ggccgcgttt
     1321 cactgattgc ccaaaatgcc gccatcctgc ctgactggac atctgacggc aatacgctaa
     1381 tttacagttc cacaaataac ggcggcatac agcctccacg cttgacttat acgcaggata
     1441 aaaaaagttg gagaataaat acttcagacg aaatatatga tgtttcaggc ggcaacatgt
     1501 catatgaata taaaaatggc aaattttccg ctatcggcca taatgtcact attatatcgt
     1561 cgacatcagg gaataaactt ctcgccgaaa cgttcattta tgtctatgac cagaacaatt
     1621 ctgcggcaag cagcacacct tccgacaaat tacaactggg ctctatcaaa aaggtaaaaa
     1681 tatttgacga acgcggcgaa atgttcaaaa aatttcccga cgcaacggag ttagccggca
     1741 tagtgttcca cgacacatgc aagatacgct gcctgaaaaa cggctggata attttctcat
     1801 ccgtaccact ttcagtgcca gccaccactc aggaaatgcc gcaatcgcaa agcctcttcg
     1861 ccataacccc cgaattgccg tcaacaataa taaacatcat acctaaggaa acacagagac
     1921 aaatgggttc aataaattgg gacacgttcc agatgtcgcc cgaccagacg cgaatcgcgg
     1981 tactcagtaa cgacaaatcc gtaaaaatcc tgaatctgaa aacttcaaac attcagatag
     2041 ttcaaccgcc actggccggc cttgacgcac cggttctgcc gacatggcag aataacgaaa
     2101 atctctgcat actcacatgc aaagaaagtg cggatgaaaa agtttatatg gttgatttat
     2161 ggtcaaacgg ttcacggaaa acaataagca ccggctggcc gaaaatcatc atgcagggca
     2221 tcgtagataa atatcgttcc aacaaatctg cagcccccgt caaagctgcc attaaatcgg
     2281 cagagacaga gattcatatg aatatgaaat cttgtgccaa agctaccact cagccgacgg
     2341 cgaaaaagtg ccctgccaaa gtaattcata tgaaatctta tatcaagccg gccaatccaa
     2401 caactcagcc tgcagaaaaa aaccatacta ctgaaattca tatgagcggc tcttatatca
     2461 gtacaaatca attagcagtt gactgaaaaa aatcgtcggt aattaacacg tcgacattga
     2521 cataacatgg cccgtaaggg gatggcattt cgatgttatc cccttacagg atgcggattt
     2581 gtggtgttat cggacgttta tcgcaaaaaa atttaaaaaa ccgtctccga ctagccaccc
     2641 gtcgctagag tcaaagtcag tttttgccga tataagtttt accatgttat atgaacctac
     2701 aggagacagg acggaacttg accacagcag acagcctgcg aaggctctcg gcaagacggg
     2761 gtctggaaat tctcaacgcg gcgacaaacg ttatttcact cccgaacatg ccaatcgtgc
     2821 cctggtgctg gtacggcgga tagtttcgga aatagtcacc ggctacgcca ggctgctgga
     2881 aatagaagag atgatagagt ccgccgagag gaccggcagc aacgacgggt tggaacaggc
     2941 acgcaagcgg ctggtgaaga cagtcgatga cctgcagaca tgcctggacg aacttgacca
     3001 gataggcgtt gaactgcggg acttttcgcg cggcatagtt gatttcccgt cgatacacga
     3061 ggatagggaa atcaagttat gttggctgct tggcgaggaa agcgtatgtc actggcatga
     3121 ggcgggagcg ggcttcgcct gccgccaacc tctctgcctg ctgggtaaaa acgaaatact
     3181 tcagccgaca cttttttaaa atgaccaatt tgcgttataa aggttcgcta aaatttttta
     3241 aaatgaccaa tttgcgttat aaaggttcgt taaaattttt taaaatgacc aatttgcgtt
     3301 ataaaggttc gctaaaattt tttaaaacga ccaattcgcg ttataaaagt tcgttaattt
     3361 tttttaaatg actgccattt ttcttccttg ccagcccgca aaattgctct atttcggcga
     3421 cgcccatgtt tttgggatag gttcaataaa aaaattttga gaaacgctcc atatgtttcg
     3481 ttacgaccaa gagtggaaga gcagattcaa aaaaatttca aaattgcatt tctgcgtcgc
     3541 aatttctaaa atacggccat gataaaggtt gctgcaaatt ttattttgtt ggccgttctg
     3601 ttgttttcag tgtcgccggc ggcggctatt tccgacgacg aaaaccttca gaaagtttac
     3661 gccgcactgg aacttgggca ggctgaccat gacgtgttgg ccaagctttc cgacggcacg
     3721 gaaaacatcc atgaaaacga gtttttctgg atgatgaaga aaatctccgc cctgcctagc
     3781 ctttcacagg atgaactcaa ccagctggac gccccagcct ggaaaactct aatatcagcc
     3841 ccgcagcggt tcagatttct gccggttcgc atgaaagtct gcgtatatac gctacaaaaa
     3901 ctttcagttg aaaaccggca tctgattcgt tcgccatatt ggccgtccga ccggcccatc
     3961 taccgaattc acgccgtaaa aatgaaaggc aacgagccca acccaaatga gccgctggtt
     4021 attttcagcc agactctgcc gccgaatctg ccggaaaaat ccgaacttca ggcggacggc
     4081 caaactgcat ataagacagg gccgctatat gagatagccg gcgttttcta caaatatatc
     4141 gaaaccggaa attcgaccgg cacttttgta aacaagtatc cggtaatact ggcgtggcag
     4201 atgaatcctg ctaaaattca ggcaccccaa ggcgcactga gcaggtttgc attcggcata
     4261 gtcatactaa taatcgcttt ggcgattgcg gttttcatgt tcctcaagcg aaggattcgc
     4321 agccagaaga aaggcttcgg atacgaaacc ctcttcagcc atgacaaatc cagaaccgaa
     4381 agcgaagaag acattgatcc gatgtcagcc atcccgaatg aactggcaga cgccgccaga
     4441 gagtatcgtc tggaacatcc cgacgaacca gccgacgcga agtgaaagga atgccctccc
     4501 gtgacatgta aaaacgattt gtcagtagac atcgccggcg taaatctcca aaacccgctc
     4561 atgaccgcca gcggcacatg cggctatgcc gacgaatacg gcgatttcgt caatctgcgg
     4621 gaactcggcg cgttcgtgac caaatccata agtctcgctc cccgcaaggg caatgattat
     4681 ccgcgaatag tggaaacccg cgccggcatg ctcaacgcga taggcttggc taacgtcggc
     4741 cttgacacgt tcgtcaatga aaaagtgccg caactggaag ccctcggcct gccggtattt
     4801 gtgaatatcg caggcacgac cccggatgat tacgtaaagg tggcagccgc acttgaaaac
     4861 gtcgacatcg taaaaggcgt ggaactgaac atatcctgtc cgaacgtcaa agccggcggc
     4921 attcagttcg gcgttcagcc ggaactcgtg gaggaaatca cgcgaaccat aaaatccgtc
     4981 tgtcgcaaaa atatcctcat agtgaaacta tcgccgaatg tcacggacat aaccgccacc
     5041 gcccaagccg ccgtcgacgg cggggctgac gcattaagca tgataaacac ttttacggca
     5101 atggttgtgg acattgaaaa acgtcggccg gtgttggcta accgcacagg cggcctttca
     5161 gggccggcta tacggcctat cgcagtctat atggttcacc gcgtctatac gcaggtggcc
     5221 aagccggctg gcattccaat aatcggcatg ggcggcatcc agtacgcccg cgacgcaata
     5281 gaattcctgt tggctggcgc cacggggctg gctgtcggca cggcactttt cgtagacccg
     5341 cgatgcattc tggaaataaa acagggcatc gacgaatatc tcacgaggca taagaagggg
     5401 tcaatacgcg acattatcgg cacattggaa ggctgaaatt gtaaggaggc ttaaccacat
     5461 tctgtcaatt aggggaaatc gcggataatt ttgaacaatt cagaaagttt ttaagaattt
     5521 atttcaaaat aatttataaa atcctgctac ataaatgtag gcggcaaaaa tctttcattt
     5581 cgggacaaaa gaaaaaaatt tcacataaaa tattttccgg attgaatttg aattgaagac
     5641 gacgacgctc ctatatataa ttatggacac aactggagtt gaaatagtta aaagggtatt
     5701 gccatgaggg tattcatgct gggctgggag ttcccgccct ttattagcgg tggcttgggg
     5761 acggcttgtt acggcctgac gaaagggctc aacgagatag acgttgatat atgcttcgtt
     5821 ctgcctacag ccatcccaat agacgcgacg acaaccggca agactcatgt gcaactgcgg
     5881 agtccgtcgg atgttgagcc tgcgcgcctt cagcggctga tgaaccccct gtccgaagag
     5941 gaagaagaaa caactctcga agtaacccat aacaggcgaa ccctgcatac gcgaaagcat
     6001 catcatctca gggttgaaca tctgccggca tttctgatgc cctatgcaac gcccgaaacc
     6061 tatgaaaaaa acatcgtcga gcttcgtcgg tacgccagaa attccgcata taagcaggcg
     6121 ttacgttatg caatcgccaa ggctaacggc aaccatcact gcctggagaa ctggcatttc
     6181 ccgctcgaga cctctgccgt cgactccgac gacgcaaata aatccataat ccagggcaca
     6241 gccggcgcac actacgaagg cgatcttttc gcacaggttc accgttttgc atcgctggcg
     6301 gtggaactgg ccatgcgtga agatttcgac atagttcacg cccacgactg gatgacgtat
     6361 cccgccggca tcgccgtggc gcaggccacc ggcaagccgc tcgtggttca cgtgcacagt
     6421 acggaattcg accgttccgg ccagaacgtc aaccaggtga tatacgacat agagcgggca
     6481 ggcatgacgc tggcgaaaag cgtggtctgc gtcagccacc tcaccgccaa catcgccaag
     6541 caccgatacg gcgtgccgga aaacaaaatc accgtcattt acaatgcggt cgaactgccc
     6601 gccaggccca acggcccatt gaagaccatt gccaaaaacg aaaaaattgt cctgtttctc
     6661 ggccgcgtaa ccatgcaaaa aggcccagaa tacttcctgc aagccgccaa aagggtcgtg
     6721 gaaaaatata aaaaagtacg tttcatagta gccggcagcg gcgacatgat tacgcgctgc
     6781 atcaagatgg ccgccgattt gggcatcggc aaatatgtga cgttcaccgg cttcctgcgg
     6841 ggccaggacg tagacagggt attcaggatg gctgaccttt acgtcatgcc cagcgtatcc
     6901 gagccgttcg gcattgcccc actggaagca ctctcccgcg atgtgccggt cataatctca
     6961 aagcagtccg gcgtttcgga aattctgacc cacgtcctga aggtcgattt ctgggacacg
     7021 gatgaaatgg ccaacaaaat cctggccgta ctccgccatg agccgctggg gaaaaccctc
     7081 cagcaacaca gcaaaatcga actgcacgga atttcctggc acgatagtgc attgaaactc
     7141 aaagaacttt acgctacact agtgtaaagt taagcgagtt ttaaaaagac tgtacgggaa
     7201 taatctgtgg cggcttcttg gtgaagcggg caaagtcaaa tcccgtcgca gccggataat
     7261 aaaatgagga gacggagcag ggatggcatc agtcgtattt tattttcagg tgcatcagcc
     7321 gtacaggctg aggcgttaca gcatttttga tacgagcgaa aattacttcg acgattacag
     7381 caacgagcag attttaagaa aagtagcgga aaaatgttat ctgcctacga accggctgat
     7441 gctcgacctg attcgcaaac atcagggccg ttttcgcata tgttattcaa tcaccggcac
     7501 aatactcgaa caatcccagc aatggatgcc ggaagtcgtc gattccttca aagcgttagc
     7561 cgacaccggc tgcgttgaat tcatcgccga aacctatttc cacagcctgg cgttcctcta
     7621 cgaccgcgac gagttccgtc gccaggcaaa gatgcactgt cagctgatgc aggatctttt
     7681 cggctattcg cccaaagttt tccgcaatac ggagttgacg tataacaatc aggtcggcaa
     7741 gctggttgag gatatgggct tcaatgccat cctgaccgaa ggcgccgact tcgtgctcgg
     7801 ttatcgaagt cccaacttca tttaccatcc gccggcctgc aaaaaactca agctgatgat
     7861 gaaaaactat cgtctcagcg acgacatcgc gttccgtttc gggaatcgtg cctgggcgga
     7921 atggccgttg actacggaga aattcgccgg ctgggtagac cagattaacg gctgcgggta
     7981 tacctgcaac ctgttcatgg attacgagac gttcggcgaa caccagtggg ccgacaccgg
     8041 cattttcgac ttcatgtatc atctgcccga cgcaatattt gccggcggca agaatgattt
     8101 tctgaccgtc agccaggcta tcgcacgcta tcccgacgtc ggtgaagttg acgtgcctca
     8161 catgatatcg tgggctgaca cggaacgtga cctgtcggca tggctgggca attccatgca
     8221 gtccaacgcg ctgacggacg tctacgaact ggcgtcgcag gtgttagcca ccaaaaacaa
     8281 acggctgata cacgactggc aaaagctcca gacgagtgac catttttatt acatgtgcac
     8341 caaatatttc tccgacggcg acgtgcacaa gtattttaat ccatatgaat cgccctacga
     8401 cgcatatata aactacatga acatattgga caacgtgcgg gccaggattg cccaatgtca
     8461 gccggcgacg gtttgaatgt ttgatacgcg gctgacgtcg gcggcagttt tataattttt
     8521 ttacaatttt tttacaattt ataatcggcc gaaattagga tacgtcaaac caatgaacgt
     8581 aaatacaatg aaaaatctcg cgacgttttt ttgcggcatg gccgcattaa tagccggctg
     8641 cgacggcatc agtcccaaag gcggcaatgt cgaaacctgc gaaaaagtgc cggacccgat
     8701 atcgctgctt ctgccgcaaa aaatctccat tcatcccttc acgcagacgg ctaccttcga
     8761 agacggcaaa cagggcctgc acatgcggat acaatttacc gattttttcg gcgaccctac
     8821 caaggcgttc ggcgacctgc gatttgaact ctacgaattc aagccctacc agcagcacaa
     8881 acgcggcgac cagctcgata tctgggacga atatatctcc gacccggaca agaacatgat
     8941 tcactgggac agccataccc gcagctatga attccgcctc gcatccaaga atcccatcct
     9001 gccgggccag aagttcatac tcgtggttta tttcaacagc cgttttacgc ctcgcatgac
     9061 cgcagaaaca gttttaacgg ctgaatgacg tagcagaact atttgtcacg catatcgtaa
     9121 ctgcttgcaa aataagtcgt taccgaaccc tataacgcga atcggtcact ttgagttttt
     9181 aaaaaaatct tctgtcggct gattttgtcg tccgcatttt ccagaggagc tgattttgtc
     9241 cgacaattcg cccagaccgt tagccttaat aaccggcgcg tcgtccggta taggggctga
     9301 gttcgcccgc agcctggcaa aacgcggctg tgacctgata ctcgttgcaa ggcgcgagga
     9361 caaacttcgc aaattagccg acgacatcca ccaagactgc gacgcagcca ttgagatttt
     9421 tccagccgac ctcgccgacg acaaccagcg acatgccgtg gcagacagga taaggcagac
     9481 gggcggtttg gagtatctgg taaacaacgc cggtttcggc acaaaagagc gtttctggca
     9541 ggctgacttc gccagccagg atatcatggc tcggctgcac gtattagccg tcatggaact
     9601 ctgccacgca gcattgggcg acatggtcaa aaaaggcggc ggcaacatca tcaataccgc
     9661 cagcgtcgcc ggatttttcc aaagtccatc caacgtcgtg tattgttcaa ccaaggcatg
     9721 gataatcagt ttcacggagg ggttagcctt cgaactcgtc ggcagcggag tcaatgtcca
     9781 ggcactgtgt ccgggctata cgctgagcga atttcacgac gtgatgcaga tatcccgtga
     9841 cggaattccc aaaagctggt ggctgccggc aaacgtggtg gtgcatgact cgctggctgc
     9901 gctcgccagg cgcaaacttg tctgcgtacc agggttgtgg cggtacaagc tgttaatcgc
     9961 tatcagcccg tttctgccgc gatggctgaa atacagggtc gcagcagcca gacataaacg
    10021 cgtaataaaa accggcaaac ccataatgat gtaattttcg agccaccttt ttataaaaca
    10081 gacttgaata tgtaccgctc atttcctata taatatggtt aagttttgtg ggactcccaa
    10141 gcgttaggga gtatttaagt ttaatcaggc tttcgagcca tagttttaaa ggaatgttca
    10201 aatggcacac gttatctccg acgattgcac caaatgcggc gcttgcgcag ccgtatgccc
    10261 tgttgaggca ataagcgaag gcgaaaccaa atatatcatc gaccccgaca catgcgtgga
    10321 ttgcggtgcc tgcgcaggcg aatgccctgt cagtgcaata agcgcacagt aaatcgcatg
    10381 tcgcaaaatt cccaaagccc cgataatata tcaattatcg gggctttttg ttcgtagcac
    10441 aaaaatgtcc atatatgccc ttcttggtat agcagtaggc ttggcgatgg acgctttcgc
    10501 ggtcgccatc gcagccggtt tgacattgcc gaaggtgacc cttcgtcact cattgcggct
    10561 ggcgtggcac ttcggcgtat ttcaggctat catgccgata atcggttatt tcgccggcaa
    10621 aaatttcgcc ggcaatcttt tttataaata cgaccactgg atagcatttt ccctgctaac
    10681 tctgctcggc ggcaaaatgc tgtacgaagc gttcacaagg aatgccgaca gtgaaaaatc
    10741 tgacccgacg aaaggctggc ggctggtgac gctgtccgtc gcaaccagcc tggatgccct
    10801 ggcagtgggc gtcacgatgg cattgatgaa aacagaaatt tttacgccgg cgttaataat
    10861 cgggatagtg gcgttcatat tcaccattct tgggattcgc ggcggcagtt ggctgggtga
    10921 gaagtgggag catatttccg agttcatcgg cgggctggtt ctgatactca tcggcgtaaa
    10981 aatcctcgcc gacagcctcg gctggctggg ctgagcccta ccggatttta gccagcacct
    11041 tgagcgccca attcacgctg gccatcggaa ctatcgtcgg tttgtaatct gcattggcag
    11101 ccaccatgtg aaggccgtcg tcggctggat gataaatctt gcatgtgacg ccgatagccc
    11161 ccgcaagctg aataacagcc ggctcgccga caaccgccct gacggacgga ctcagcacga
    11221 ctatatcgcc atgaaaaatt tccggcgaca tggaatcgcc gtcaatcctt agagcgaagg
    11281 catcgggata tttgcggcag atggcttcgc tggcaatgaa ttccacggca ccgcccggca
    11341 catcgggcct gtccagcgtg attatctgaa cgccctgctg aacgtcgccg gaactggccc
    11401 ggctgacagc cgctattttt tcatcccgca cctcgccgac atgtttttca atcaaatcgc
    11461 ccagtgtgac gatatcggcg acctgttcgt cgtctgccca gaaatgcgat atgcccgccg
    11521 cacttctgcc gagaatggga atccagcccc gccgtccgcc gccggctgaa gcgacttttc
    11581 ctacagcccc aaaggcaaca ttttcagccg ccagatttcc ctgccccaca tcatcaaccg
    11641 aattttcttt cggcggaaaa gcctttatgc cttcgagtat ttcaagaaac gccgccaacg
    11701 gccgtgctga cgagggctgc tcccgcaata gccgtgccgc acgctgaact atcggattgt
    11761 tgacatccaa gccgtcagcg gactttctgc ccgtaagcag ccagccaaga tccacgcctg
    11821 ctacctcact tatgcgaata agaacgtcaa ccggcggcac acgggaagcc tcataataat
    11881 cgtaagtgct cggcgacaga cctaactgtt ttgcaaaacg agacttacca cgtggaccag
    11941 catattccag gcgtatctgg gacactcttt tgcaaacccc tttgagtaat tcttcttccg
    12001 ttggcggcat aaatgaccca ttattttttc gcttttgcga aaattttagt tgtaaatatt
    12061 cgctattgtg gataaaatta ccccaaaatc acagacgggt aaaaccgggt ggattatatc
    12121 attttttgtt ttttttacaa gtattacggg gaacgccatg aaaaaaagct caacaaatat
    12181 tggccgacgt aagggagcaa aaacacataa gttcgacgac aagtcgtcaa gcaactccgc
    12241 tgaacacaac gcttcagcca acaacctgtc cgcaccacac gcagccagga aaggcataac
    12301 atcggccaaa aaactgccgg gccatccggt tcgtaaggtc gacgcacgtt cgctacacag
    12361 gatattgacg tggtcaatag aactcagcca gcggcaacag caacttatgg acgccgcaat
    12421 tgaaatactg gctaacgcaa aaaacgactg ataccaattc aaaaaatgag tgcaatagag
    12481 catctcaaat ttgagattgt ggcttagtgc gttatcaaga acaaggctga caagaaaggg
    12541 aagcagaagt ccctgagtta ttttcgccgt ctgacggcaa atactgcacc catccctgcg
    12601 acaagcagta caagcgttgc cggttcagga accgtatcgc cactgacggc catgatgata
    12661 ttgtgctgcg gcacatcagg gtcggatgga acagcattat ccacaatcac tattgattgc
    12721 agcaggcgtg tttcgtcgac atcaaagatg gtttcgtaaa gacgcgggtt accggaatta
    12781 acatactcga agtcgccgct gacagtgctg attcggccat tggcggtata ggcaatcgac
    12841 ccgccgccaa accagtcatg gttgatggaa tccgtgccca gactggaata tacgtctgga
    12901 tcgccggtcg tatagttaaa cgtgatagta aggttcgccg tcccctggcc ggtcgcaccg
    12961 attagtgcaa tgtggtcata cgccgccaca gtcaccagcg tcagagtgcc tccgccataa
    13021 acggcgttat tattcgttcc ggcggctgag ccaaaagatt ggagctgaaa ataaacatcg
    13081 gaagaggttg cactttgtat caggccagtc ggcagaccag tcgtaggata gcttgtgttc
    13141 aatccaaccc cataccatac cattgcaccg cctgtagttc cgctggcatt gtctaattga
    13201 gccgtgcttc cggtattgta tgtttcacca acccctataa cgacatcctg attccagcct
    13261 gttacagaaa ttggattaag cgttccagcc tgcacaaaag aacactgaaa aacaatagcg
    13321 caaaccaaaa gccctgcgga aaaagaacgg acacaagccc gcatagcata atccctcgat
    13381 taggctaatt tgggaaagca aggattttac aataggctct ctcgacctca ctttccaata
    13441 atttaattat aagtacaacc ctttttaaaa taacacatta aaattaattt ttcaaaaaat
    13501 tttattatcg gcatcattac atatttaaaa attttgcgtt tggtgaaata gagcatgtta
    13561 aacttgagat gtgacacttt atgcgttggc gaaacgagct atgcaaggaa ggcgaagcag
    13621 gaatact
//
LOCUS       DAONFI010000013        12859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_5421_length_12859_cov_4.136442, whole genome shotgun sequence.
ACCESSION   DAONFI010000013 DAONFI010000000
VERSION     DAONFI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5421_length_12859_cov_4.136442"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1001)
                     /locus_tag="PLK08_00945"
     CDS             complement(150..1001)
                     /locus_tag="PLK08_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPS52095.1"
                     /translation="MITFALLPAAAQEVSTTVEPTKVEAFTKPNFDAILSFAVPGTIA
                     EISVEIGQKVKKGQVLARLEDSVEQTRQAQLQAQAENTIKIKAAAAQLDQKKVDLERT
                     KAAAKDGAATELEMEYARLDVLTQELTKQIEEFNNSQDKLKNKEMKSQVDRMKIVSPC
                     DGIIETRFVEKGQAVDSNTQAIRVVNIDPLWVDVPLSVNSTMSLKIGGKMKVRYLNAP
                     AEVCQAEILNVASTVDPASDTRLVRLEIPNPSKRPAGERVEVLLDENQTQTRTTAKTQ
                     PDKNNSK"
     gene            complement(1077..3071)
                     /locus_tag="PLK08_00950"
     CDS             complement(1077..3071)
                     /locus_tag="PLK08_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52096.1"
                     /translation="MTVTSDIYSWRVFTRGYKTDELPTGIDILWDRTKGFLNSFRFRT
                     GRFLKLADSVLELNQDMADLSDASLRQTADRMREIFRLNRDTPADRIRGLAVVREVAR
                     RIRNEHPFRVQVAGAMAMLEGCITEMATGEGKTLVAALVATLEGWRGKGCHVLTVNDY
                     LAQRDSESMKGIFEFCGLTCGFVNQEMSPPQRKESYDCDITYLTNKEVTADFLRDQLK
                     LGRRRKLLDRIVDSMDIYSRQVGRDMLQRGLYSAIVDEADSILIDEAVTPLIISSNSS
                     DPIQADAFSRACEIAASMTAGLHYRVNHQYQEITLTGEGRDKLQNVTAGLGGIWAGER
                     RSEELAVQALTAREFYESGRQYIVDDGKVVIVDEATGRLMPDREWRNGLHQAVTAKEK
                     LEVQPPKDTCARISFQRFFRLYEKLCGMTGTAREASWEFWDIYKMPAVRIPTNRPCRR
                     IKLPTRVFSDADDKRDAIVAEVRKMHSQGRPVLVGTRSVKESVSLSEKLSDAGLKHQV
                     LNAVYHKIEAQIIASAGNSGQITVATNMAGRGTDIKLGREVEKLGGLHVIATEKHFSR
                     RVDRQLQGRSARQGDPGSAVEIICLDDELFVRYAAIFRMLLKPFVGKKSAGKIVRMAA
                     ILAARLGQFRAGRQALAMRKSVVKTDNWLDETLGFAGKEL"
     gene            complement(3068..5269)
                     /locus_tag="PLK08_00955"
     CDS             complement(3068..5269)
                     /locus_tag="PLK08_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52097.1"
                     /translation="MAQVRPTFHESWYRIAQLRPRVRVTVQIQKQYYRGVGWYIIRDP
                     ANNDHFRLSESGYAFIALLDGYRTIEQAWKICLEKLGDDAPTQGEVLQLLGQLYMSNL
                     LCGDMPPDCESLFARYKKRKLREIGATLGNILFLRLPLFDPDRFLEGFEKVFGWIFSW
                     FGFALWAVLVVMGLAAVAGRWSDLVSQAGNVLAPSNLVFMYLCFVVIKAIHELGHGLA
                     CKKFGRLEGDTGEVHTIGIMLLVFTPMPYVDATSSWSFRDKRRRIMVAAGGMYVEIAV
                     AAVAAMLWAASASGSILHALSYNTMFIASVSTLLFNINPLLRYDGYYILSDILEIPNL
                     YDRSKRYLYYLIRRYVWGIDSIINPAHTRGERIWFLPYAVASCIYRVIIFAGILLFIA
                     DKLFFVGVILAMCMAVAWLVWPAVKFVSYLATNNELARHRQRAVLTTVLFFGMIFSLL
                     GEYEWPDRFSAEGIVEPEKLTLVYSGTDGELVSHMKTDIAVKTGDGPLITLKNPELTS
                     KYKSLLAELEESGLRKKIAQSENRTAEAAIYDSQSKALTDQLARVKTELSRQRIFAAT
                     DGVWLAPRLSRQEGKWFKRGELIGMIVPADSMRIRVAVSQEEAARLFSSGGIRVSYRF
                     DGASDSKTGKGNIVQIVQAGRKQLPSAALGYAGGGEIETAASNPQDRQGAYEATHNYF
                     EVLVTPARNAADIADFREGRKVLVRFDVGNRPLLSQFYRKIMQVFQQRFRQ"
     gene            complement(5285..7153)
                     /locus_tag="PLK08_00960"
     CDS             complement(5285..7153)
                     /locus_tag="PLK08_00960"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPS52098.1"
                     /translation="MIDNLAARHISDADPQEEIHRLIRFEGTFEEFLCKWLSAVCHCA
                     SASQAAVLDRDETHGNIVAKAIFPSTEVGGGMPFWLQQFAAQLGRNADWPAECSIHDI
                     PVPGGFYGQTQKVFAYPLGIDGTKLIAAFLISGECDSKRLAAITAPFTCLINNYVAGR
                     KVSVQENSLRRFRKLFELQSSLRHHARFGPLAMAICNEIATAAGAERVSLGVLGGHFV
                     KCMAMSHTEKLNRKMKVVRQVEQAMEECLDQQQVVIYPAGDGDRAVTRMAEELSRHHG
                     SGQIIAIPITPADEPKIVILMEFTDRRNDLKELLEITGAICEVEAARLYEFYLHSCWF
                     GIRIAREIKKFAAIVVGAKHTWLKLAAIAIICVLFWAVFFRGDYEIESPVVLEAVQQR
                     VVAAPFECYLVEADVQPGDYVQAGKTMLGRLDDGELRQQLASLEARRQTSVKRAAMAM
                     REGKTAESQVELAQAKQAQADMDIVRYKLSKVNIVAPTSGYLVSEDMGKQLGRAFKIG
                     DVLFEIAAVDSLRAVIRVEEEDISQLSVGQSGEFAIIANPDKKLRFTIERIHPAANVV
                     EQKNIFEVWAKIEERPTWLRPGLEGEAHIYVGKRRHIWIWTHRAADWIQMKLWQWF"
     gene            complement(7191..7511)
                     /locus_tag="PLK08_00965"
     CDS             complement(7191..7511)
                     /locus_tag="PLK08_00965"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPS52099.1"
                     /translation="MVEENAQNQQVKLTIDQRDMHTFYANAFQTNVTPEELFVSFGTN
                     QPIPAQDGSKQPQIMLKLESRIAMNYYTAKRLAIQLSQAVQGFEERFGVLELDINKRT
                     QGKK"
     gene            7799..8833
                     /locus_tag="PLK08_00970"
     CDS             7799..8833
                     /locus_tag="PLK08_00970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS52100.1"
                     /translation="MSQPKRIFLVADFKHGRARAIRAENRHWIKGFMRLGHDVQCFSY
                     IDMVAQLAPVKFGGIMLKLGKKKADEVIAEQIRYYHPDIVMIITMKDMDGETVAGMKK
                     VAPNAIFVGRDIDWFLESDINKIEVAKKMDIVMNTDAGEFLKAYKKLGVPRCAFIPCL
                     CDPDIQRPYDVDEKFRSDIIFAGKATHGQGECDDDRFAIVERLSRMPNAKVYGAFGQP
                     AIDGLDCFYALGGAKIALSINAVNNVRLYHSDRFINSLSCGAFVLAKRVPDTDMLFQD
                     GVHLRYFDTADEFFELADWYLKHDAERVKIARAGMERAHNEFNCVKMAEHAISLIEKG
                     SYDVPWATVL"
     gene            9074..9859
                     /locus_tag="PLK08_00975"
     CDS             9074..9859
                     /locus_tag="PLK08_00975"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPS52101.1"
                     /translation="MIGLGVNIDHVATIRQARRTVEPDPVWAAAAAELGGASCITFHL
                     RKDRRHINDRDAGLLRRTVRGKLNMEMSLDEEIVRIALELRPTQATLVPENRQEITTE
                     GGLDVVSQFERFSEVTRRLNDAGIMVSAFIDPVESQIIASRNAGAQCVELHTGTLANA
                     ILPPDGSKASQEAVAGAFADVRNGLAVGLDQGLIVHAGHGITYQNVHILAALRGFTEF
                     NIGHTIIARGIFTGLREAVAEMKRLIDRSQSAPEPLELERIAL"
     gene            9856..10728
                     /gene="dapA"
                     /locus_tag="PLK08_00980"
                     /pseudo
     CDS             9856..10728
                     /gene="dapA"
                     /locus_tag="PLK08_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
     gene            10850..>12859
                     /locus_tag="PLK08_00985"
     CDS             10850..>12859
                     /locus_tag="PLK08_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPS52102.1"
                     /translation="MSIESHEILILDGACGSTLQGMDIPDSAWAGREGCNEWLNIAAP
                     QIVQEMHSKFLAVGTMAVETNTFGANSIVLEEYGLADRVDEINRAAVANAQAAVVAAD
                     NAAGYVIGSVGPTTKLPSLGHIAVDKMHEAYVGQLRPLIDAGVDALLIETCQDILQTR
                     IALIAAQQVMNELNREIPLMASVTVEQSGKMLVGTDIATAVTTLEPFGLFSLGLNCAM
                     GPDLMEPHIRTLGKLWPGRISLLPNAGMPQVIEGKTCYMQQPLPFAKKMREFVTQYGV
                     SIIGGCCGTRPDHIKAMVDALGDGVKPAMRCPEFQPSLSSAYQSVTIEQQPRPFMIGE
                     RANANGSKNFRELLLAGDFDGALQVGLDQQATGAPHALDLCVAFAGRDEKADMERLIK
                     MFANSIRLPVVIDSTSPDVIETALKNYPGRALINSINLEDGGTNLRKICRLAKKYGAA
                     CIALTIDEKGMAMTADAKLAVATRIYDIAVCECGLRPSDLVFDVLTFTIGSGDESTKN
                     AGVETLAGIRLVKRDLLGVFTTLGLSNISFGLKPASRRILNSMFLHEAISAGLDTAIV
                     DAARILPLNQMDARDRAVALDLIYNRVSEKNPDPLARFIERFNDATVRQQTQEPAKDR
                     RPAEELLTEHMFAGKRDGLEDVIAALLEKYAPTEIINNLLIEAMRI"
BASE COUNT         2772 a         3384 c         3479 g         3224 t
ORIGIN      
        1 gccaggctgc aaaagagccg attgcaggca gccaaggttt ttgggatagg ttttagaacc
       61 gctctgtcca tattattcaa gccgaccgat ttttgaagtt tatgcgtgca tatgccgtcc
      121 atgctgttga acctgacggc gttaatcggt cactttgagt tatttttatc aggttgcgtt
      181 ttggcggtag ttcgagtttg cgtctgattt tcgtcgagca atacttccac tcgttcgccg
      241 gctggccgtt ttgaaggatt gggaatctcc agccgcacaa gccgcgtatc gctggccggg
      301 tctactgtcg aagctacgtt cagaatctca gcctgacaga cctccgccgg tgcgttgaga
      361 tatctgactt tcattttgcc gccgatttta agactcatgg tgctgtttac gcttagtggc
      421 acgtcaaccc agagcgggtc tatgttcacg acgcggatag cctgcgtgtt gctgtcgacg
      481 gcttggcctt tttcgacgaa cctggtttca ataatgccgt cgcacgggct gacgattttc
      541 atgcggtcga cttgcgattt catttccttg tttttgagtt tgtcctgcga attgttgaac
      601 tcttcaatct gttttgtgag ttcctgggtg agcacgtcga gcctggcgta ttccatttcc
      661 agttctgttg ccgcgccgtc ttttgccgcg gcctttgtgc gttcaagatc gacttttttc
      721 tggtcgagct gggctgccgc ggcctttatt tttatggtgt tttcagcctg cgcctgcaac
      781 tgcgcctggc gggtctgctc gaccgaatcc tccagccgcg cgagaacctg gcctttcttt
      841 accttttggc cgatttccac ggatatttcg gcaatcgtgc ctggtacggc aaaagataga
      901 atcgcgtcga aattcggctt ggtgaaggcc tcgacctttg tcggttcgac ggttgtggat
      961 acttcctgtg ccgccgccgg cagcagtgca aacgtaatca tcaaaaatcc ggccgtcata
     1021 aaaaatttgg ttttcgtttt catgttaaaa actccattgc cgcgattacg gcgtcgttat
     1081 aattcttttc ctgcaaatcc gagagtttcg tcgagccagt tatcggtctt gactacgctt
     1141 ttccgcatcg ccagtgcctg ccgtccggcc ctgaactggc ctagccgcgc agccagtatc
     1201 gcagccatgc ggacgatttt gccggctgat ttcttgccga cgaacggttt taacaacatt
     1261 ctgaaaatgg cggcgtaccg tacaaaaagt tcatcgtcca ggcagatgat ttcgactgcc
     1321 gaaccagggt cgccctgtct tgcgcttctg ccctgcaact gtctgtccac acgtcgcgaa
     1381 aaatgctttt ccgtggcgat gacatgcaag ccgccgagct tttctacttc gcggccgagt
     1441 tttatgtcag tgccgcggcc agccatgttc gtggctacgg ttatctggcc gctgttgccg
     1501 gctgacgcta ttatctgcgc ctctatcttg tgatagactg cgttgagcac ctggtgtttc
     1561 aatccggcgt ccgagagttt ctcgctgagc gataccgact ccttgacgct tcgcgtgccc
     1621 accagtaccg gccggccctg cgagtgcatt tttcgcactt cagccacgat tgcgtcgcgt
     1681 ttatcgtctg cgtcgctaaa gacgcgggtt ggcagtttta ttcgcctgca cggccggttg
     1741 gtgggtattc gcaccgccgg cattttgtaa atgtcccaga attcccagct ggcttcgcgg
     1801 gctgtgccgg tcatgccgca gagtttttca taaagccgga aaaacctctg aaaacttatc
     1861 ctggcacagg tatccttggg cggctgaact tccagttttt cttttgcggt tacagcctga
     1921 tgaaggccgt tacgccattc gcggtctggc atcaggcggc cggtggcttc atcgactatc
     1981 accaccttac catcatcgac aatatactgt ctgccgcttt cgtaaaattc cctggcggta
     2041 agcgcctgaa ctgcgagttc ttcgctacgt cgttcgccgg cccatatgcc gccgaggccg
     2101 gctgtcacat tttgcagctt gtctctgcct tcgccggtga gggttatttc ctgatattga
     2161 tgatttacgc ggtaatgaag gccagccgtc atcgacgcgg cgatttcaca agcccggctg
     2221 aacgcatccg cctggatggg gtcggacgaa ttgctggaaa taatcagcgg cgtgacggct
     2281 tcatctatca ggatgctgtc ggcttcgtcg actattgcgc tataaaggcc gcgctggagc
     2341 atgtcacggc cgacctgccg gctgtaaatg tccatcgaat ctacaattct gtccaatagt
     2401 tttcgtcgtc ggccgagctt cagctggtcg cgaagaaaat ccgccgtcac ttccttgttc
     2461 gtcaggtacg ttatgtcgca gtcatacgac tccttgcgtt gcggcggaga catttcctga
     2521 ttgacgaaac cgcacgtcag cccgcagaat tcgaaaatcc ccttcatcga ttccgaatcc
     2581 cgctgtgcga gataatcgtt gacggtgagt acgtgacagc ccttgccccg ccagccttcc
     2641 agcgtggcga cgagtgcagc caccagagtt ttgccttcgc cggtggccat ttccgtaatg
     2701 cagccttcca gcatggccat tgcgcctgcc acctgaactc ggaaaggatg ttcgtttcgt
     2761 atgcggcggg cgacttcgcg aacgacggcc aagcctctta tgcggtctgc cggagtgtcg
     2821 cggttgagcc tgaaaatttc ccgcatgcgg tcagccgtct gccgcagcga tgcgtcggaa
     2881 aggtcagcca tgtcctgatt cagttcgagc acgctgtcgg cgagttttaa aaatctgccg
     2941 gttctgaacc tgaacgaatt taaaaatcct tttgttctgt cccacaatat atctatgccg
     3001 gtggggagtt cgtcggtttt gtagccgcgg gtgaatactc gccatgaata tatgtctgac
     3061 gtaacggtca ttgcctgaat ctctgctgga agacctgcat tattttgcgg taaaactgcg
     3121 aaagaagcgg ccggttgccg acgtcgaacc gtaccaggac ttttctgcct tccctgaagt
     3181 ctgctatatc agcagcgttt ctggctgggg tgaccagaac ctcgaaataa ttatgcgtcg
     3241 cttcatatgc tccctgcctg tcctggggat ttgacgcggc ggtttcgatt tctccgccgc
     3301 ctgcgtatcc caacgctgcc gatgggagct gtttccggcc ggcctggact atctgaacga
     3361 tgtttccttt acctgttttg ctgtcagatg cgccgtcgaa cctgtagctg actcttatgc
     3421 cgcctgacga aaacagcctg gccgcctctt cctgtgaaac ggcgaccctg attcgcatgg
     3481 aatcggctgg aactatcatg cctatcagtt cgccgcgttt gaaccatttg ccttcctgcc
     3541 ggcttagccg tggggcaagc cacacgccgt ccgttgctgc gaaaatcctc tgccggctga
     3601 gttcggtttt aactctggca agctggtccg tcaaagcttt cgactgcgag tcgtatatgg
     3661 cggcttcggc ggttctgttt tcactctgtg cgattttttt tctcaggccg ctttcttcga
     3721 gttcagccag aaggctttta tattttgatg taagctccgg atttttcagg gttatcagcg
     3781 gcccgtcgcc ggtttttacg gcgatgtcgg tcttcatgtg gctgaccagc tcgccgtcag
     3841 tgcctgagta tacgagggtg agtttttccg gttcgactat gccttctgcg gaaaagcggt
     3901 cgggccattc gtattcgccg agcaggctga aaatcatgcc gaaaaacaga acggtagtca
     3961 aaacggctct ctggcggtgg cgagccagct cgttgtttgt cgccaggtag ctgacgaatt
     4021 tgacggcagg ccataccagc cacgcgaccg ccatgcacat ggccagaatc acacccacga
     4081 aaaacagttt gtcggcgatg aacagcagaa tgccggcgaa gattattaca cggtatatgc
     4141 aggatgcgac ggcatacggc agaaaccata tccgttcgcc tcgcgtgtgt gctggattga
     4201 tgatgctgtc aattccccat acgtacctgc gaatcagata atacaaatac cgtttcgaac
     4261 ggtcatatag attcgggatt tccagaatgt cgctgaggat gtaataaccg tcgtacctca
     4321 gcaggggatt tatgttgaac agcagcgtcg aaacgctggc gatgaacatg gtgttgtagc
     4381 tcagcgcgtg aagaatgctg cccgatgcgc ttgctgccca gagcattgcc gcgacggctg
     4441 ctactgcgat ttcaacatac atgccgccgg ctgccaccat gatgcgtcgt cgcttgtcgc
     4501 ggaaagacca gctgctcgtg gcgtccacgt aaggcatggg cgtaaaaacc agcagcatta
     4561 tgccgatggt atgaacttcg cccgtatcgc cctccagccg tccgaatttc ttgcacgcca
     4621 ggccgtggcc gagttcgtga attgccttta tcaccacgaa acacaagtac atgaatacca
     4681 ggtttgacgg cgccagtacg ttgcctgcct gcgaaacaag gtcagaccat ctgcctgcaa
     4741 ccgcggcaag ccccattact acgagtacag cccacaatgc gaatccgaac catgaaaaaa
     4801 tccagccgaa aaccttttca aaaccttcca gaaatctgtc cgggtcgaac agcggcagac
     4861 gcaggaacag tatatttccc aacgtggcgc cgatttcacg caatttacgt tttttgtatc
     4921 ttgcgaaaag gctttcgcag tccggcggca tatcgccgca gagaagattc gacatgtaca
     4981 gctggccgag cagttgcagg acttctccct gcgtgggggc gtcgtcgccg agtttttcga
     5041 gacagatttt ccacgcctgc tcaatggtgc gataaccgtc cagcagggcg ataaatgcgt
     5101 atcccgattc gctgagccgg aaatggtcat tatttgcagg gtcgcggatg atataccatc
     5161 cgacgccgcg gtaatactgt ttctgtattt gaaccgttac ccgtacgcgc ggccgcagct
     5221 gtgcgattct gtaccatgat tcgtgaaatg tcggacgtac ctgagccatg gccggccatt
     5281 cctcctaaaa ccactgccag agcttcatct gtatccagtc cgcagcacga tgagtccata
     5341 tccagatgtg ccttcgtttg ccgacgtata tgtgcgcctc gccctcaagg ccgggccgca
     5401 gccacgtcgg ccgctcttct attttcgccc agacttcaaa gatatttttc tgttccacga
     5461 cattggcggc tggatgtatc cgttctatgg tgaaccggag ttttttgtcc gggttggcga
     5521 tgattgcgaa ttcgccggac tggccgacgg aaagctggct gatgtcttcc tcttcgacgc
     5581 ggatgaccgc gcggagagaa tctacggctg cgatttcaaa caggacgtcg ccgattttga
     5641 acgcgcggcc caactgcttg cccatgtcct cggatacgag atagccgctt gtcggcgcga
     5701 cgatgtttac tttcgagagt ttgtatctga cgatatccat gtcggcctga gcctgtttag
     5761 cctgtgcgag ttccacctgc gattctgcag ttttgccctc ccgcatggcc atcgcagccc
     5821 gttttacgga agtctgccgc cttgcctcca gcgatgcgag ctgttgccgc agttcgccgt
     5881 catccagacg gccgagcatg gtcttgccag cctgcacata gtcgcccggc tgaacgtcgg
     5941 cttctacgag ataacactcg aacggcgcgg cgactacccg ttgctggacg gcttccagca
     6001 cgacgggaga ctctatttca taatcgcccc tgaaaaacac ggcccagaaa agcacgcata
     6061 taatcgctat tgcggcgagt ttcagccatg tgtgtttggc gcctacgacg atggctgcga
     6121 atttctttat ttcgcgggct atccggatgc cgaaccagca gctgtgcagg taaaattcgt
     6181 acagcctcgc cgcttcgacc tcgcaaatcg cgcctgtgat ttcaaggagt tccttcaaat
     6241 cgttgcggcg gtcggtgaat tccataagta tgacgatttt aggctcatcg gcgggtgtga
     6301 ttggtatggc gattatctgg cctgagccat gatggcggga gagttcttcg gccatgcgtg
     6361 ttactgcgcg gtcgccgtcg ccggcgggat aaatcaccac ttgctgctgg tcgagacatt
     6421 cctccattgc ctgttcgacc tgcctgacga ctttcatctt gcggttgagt ttctcggtgt
     6481 gactcatggc catgcatttg acgaagtggc cgccgagtac gccaagtgaa actctctccg
     6541 cgccggctgc ggttgcgatt tcgttgcata tcgccatagc cagcggcccg aatctggcat
     6601 gatgccgcaa cgacgactgc aactcgaaaa gttttctgaa tcgccgcagg gaattttcct
     6661 ggacggaaac ttttctgccg gcgacatagt tgtttatgag gcacgtaaag ggggctgtaa
     6721 tcgcggccag gcgttttgag tcgcattcgc cggaaatgag aaacgccgct atcagtttcg
     6781 tgccgtcgat gcccagcgga taggcgaaaa ccttttgcgt ctggccgtaa aaaccgcctg
     6841 gaacgggaat gtcatgaata ctgcattcgg ctggccaatc ggcattacgg ccaagctgcg
     6901 cggcaaactg ttgcagccaa aaaggcatac cgccgccaac ctctgtcgac ggaaatatcg
     6961 ctttggctac gatgttgccg tgagtttcat ccctgtcaag tacggcggct tgagatgccg
     7021 acgcacaatg acagaccgca ctcagccact tgcacaaaaa ttcctcaaaa gtgccttcaa
     7081 atcttatcag gcgatgaatt tcttcctgcg ggtcggcatc ggatatatgc cgtgcggcca
     7141 aattgtcaat catgtcatcg ggcacttgtt aagccgtctg cgacgtaaaa ttatttcttg
     7201 ccctgagttc gcttgttgat atcaagctca agtacgccga accgctcctc aaaaccctgt
     7261 acagcctgcg aaagctgaat cgccagccgt ttggcggtgt aatagttcat cgctatacga
     7321 ctttcgagct tgagcattat ttgcggctgt ttactgccgt cctgcgcggg aatgggctga
     7381 ttcgtgccga aacttacgaa taattcctca ggcgtgacat tggtctggaa tgcattggcg
     7441 taaaacgtat gcatgtcgcg ctggtcgatt gtcaatttta cctgttgatt ctgggcgttt
     7501 tcttctacca tgattttccc tttaaaaaag ccttgttgga attacacata atatctgccg
     7561 atttcaaatc gccgaatata caatatcctg cggtcaaaca ccataagtaa ttggataaat
     7621 tgtgtcaaac cctttttctg tcaaagacgt tcgcagaggc atcgtttatt cagtggctgc
     7681 cggagttgcc ggctgatttt gtaattgatt aacgttttga atttacctat cattgtcccg
     7741 taaattattc cacgaagcgt acttgttcaa attcggtctg aaatcttcag gatgccttat
     7801 gtcacagcct aaacgcattt ttcttgtcgc agattttaaa catggcagag cgcgcgcgat
     7861 acgtgccgaa aatcgtcatt ggattaaagg atttatgcgg ctgggccatg atgtacaatg
     7921 cttcagctat attgatatgg tagcgcagct ggctcccgtt aaatttgggg gaatcatgct
     7981 gaaattgggc aaaaaaaagg cggatgaagt cattgctgag cagattcgat attaccatcc
     8041 tgatattgta atgataatca caatgaaaga catggacggc gaaactgttg ccggaatgaa
     8101 aaaagttgcg ccgaatgcaa tcttcgtcgg cagggatatc gactggtttc ttgaaagcga
     8161 tatcaacaag attgaggttg cgaagaagat ggatatcgtg atgaatacgg atgctggcga
     8221 atttctgaaa gcatacaaga agttgggagt ccctcgttgt gcattcattc catgtttatg
     8281 cgaccctgat attcagcggc cgtatgatgt cgacgagaag ttcaggagcg atataatatt
     8341 cgccggcaaa gccacccacg gccagggcga atgcgacgat gacagatttg ccatcgttga
     8401 aagattatcc agaatgccca atgcaaaagt atatggcgca tttggccagc cggccataga
     8461 cggccttgac tgtttttatg ctcttggggg ggcgaagata gcgctgagca tcaatgccgt
     8521 aaacaatgtc agactttatc actccgacag gtttatcaac agcctttcat gtggcgcctt
     8581 cgtattggcc aagagagtgc cagataccga catgcttttt caggatggag ttcacttgag
     8641 atattttgac actgcggatg aattttttga attggctgac tggtatttga aacacgatgc
     8701 ggaacgtgta aaaattgcga gagccggaat ggaaagggct cataatgaat ttaattgtgt
     8761 aaaaatggcc gaacacgcca tcagcctgat tgaaaaaggc agttatgatg tgccgtgggc
     8821 gactgttctg tagtttgtga atttttgcga aaaaaatgag ggattgaaac agcttttacc
     8881 tgtctctgta atatcgtctc agccggttga ttattgaaac aattgtcaga aataagggtt
     8941 ttccagagca tgcaaagttt ttataataca tgactgccgc tgctgtcggt atgccgacgt
     9001 taatacagga aaacatgttt gaattttttc tatggtcgtg atgccgctga acggtattgg
     9061 gagtatattt tagatgatag gattaggagt aaacatagac cacgtcgcca cgattcgtca
     9121 ggccaggcgt acggtagagc ccgatccggt ctgggccgcg gcggcggctg agcttggcgg
     9181 ggcaagttgc attacatttc atcttcgcaa ggacaggcgt cacataaatg accgcgacgc
     9241 cggtttgtta cggcggaccg ttcgcggcaa gctgaacatg gagatgtcgc tcgacgagga
     9301 gatagtgcgg atagcccttg aattgcggcc gacccaggcg acgcttgtgc ctgaaaaccg
     9361 ccaggaaatc actaccgagg gcggcctcga cgtcgtcagc cagtttgagc gtttttccga
     9421 agtgacgcga cgtcttaacg acgccggcat catggtcagc gcatttatcg accctgtcga
     9481 gtcgcagata atcgccagcc gcaatgccgg ggcccagtgc gtagaacttc ataccggcac
     9541 tctggcgaac gcgatattgc cgccggacgg gtcgaaggcg tcgcaggagg cggtagctgg
     9601 cgcttttgcc gatgtgcgaa acggcttagc cgtaggcctt gaccagggcc ttatagtaca
     9661 tgccggccac ggaataacct atcagaacgt tcacattctc gcggctctgc ggggatttac
     9721 ggaattcaat atcggccata cgataatagc tcgcggtata ttcactggtc tgcgggaggc
     9781 tgtggctgaa atgaagcgat tgattgacag gtcacaaagt gctccggaac cgctggagct
     9841 tgaaaggatt gcgttatgag tctgaagctc gaaggatcaa tggttgcact ggtcacgccg
     9901 tttcatggcg aaagtgtgga ttacgatacg ttgggcaggc tgattgaatt ccagattgcc
     9961 ggcggaactc aggcgatagt gccgtgcggc actaccggcg agtctcccac gcttgattac
    10021 aaggaacatg accgtgtgat agaatttacg gtggaaaagg tggctggccg cgtgccggtt
    10081 atcgccggta ctggcagcaa ctctaccgaa gaggcatacc gcattaccgc tcatgccaaa
    10141 aaagccggcg cagacgcctg cctcatagtg aatccgtatt acaacaagcc cacgcagcga
    10201 ggcatgtatg cccatgtcgc acgcatcgcc gaagtcggtc tgccgatagt gctgtacaac
    10261 atacccggca gaacaggcgt ggaattgacc gcggaaacca tcgtcaacat gtataaccag
    10321 atagagcagg tggtcgcggt caaggaggct accggcaaac ttgatatgtc cactgccatc
    10381 gcgagcatgt gcgacattac gatactttcc ggcgatgatt cactgacgtt gccgataatg
    10441 agcgtcggcg gcaaaggtgt cgtcagcgtt ctggccaacg ttctgccggc tgaggttcgc
    10501 aaactttgcg actttgcagc cgccggcgat tacgaatcgg cccgcagaca gcatttgcgg
    10561 cttttccccc tgtgcagagc catgttctgc gagacgaacc ccattcccgt gaaagccgcc
    10621 atgtccatgg ccggcttgtg tgacgattcc ctgcgtctgc cgctctcgcc cctggacgag
    10681 cagtatcgcc cgatgctcag aaaatttttg aaagatttcg gcgccgtaaa atggtccgga
    10741 cggcagttgg cagtcgacgg tcggcagtca tgttgggtaa ccattttgaa aacgtattca
    10801 tggcggctga ctgtggtttg ccggctgcct ttttgttttg gagaatgttt tgagtatcga
    10861 gtctcacgaa attttaatac ttgacggtgc ttgcgggtcg actcttcagg gcatggatat
    10921 tcctgattct gcgtgggccg gccgtgaggg ctgtaacgaa tggctgaata ttgccgcgcc
    10981 gcagattgtg caggagatgc actcgaaatt tttagccgtc ggcacgatgg ctgtcgagac
    11041 taacactttt ggggcgaatt cgatagtttt ggaagaatat ggccttgcgg acagagtcga
    11101 tgaaataaac cgtgcagccg tcgccaacgc tcaagccgcc gttgtcgctg ccgacaatgc
    11161 cgccggttac gtcatcggtt cggtcgggcc taccacaaag ctgcccagcc tgggccatat
    11221 tgccgttgac aaaatgcacg aggcctatgt cgggcaatta cggccgctta ttgatgccgg
    11281 cgtagacgcg ctgctcatcg agacctgcca ggatattttg cagacccgca tcgccctaat
    11341 tgccgcgcag caggtgatga acgaacttaa tcgtgaaatt cccctgatgg cgtcggtgac
    11401 ggtggagcag tcgggcaaga tgctcgtcgg tacggacatt gcgacggcgg tgactacgct
    11461 tgagccgttc ggcctgtttt cgctgggcct gaactgcgcg atggggcccg atctcatgga
    11521 accgcacatc cgtacgctcg gcaaactctg gccgggccgc atttctcttc tgccgaatgc
    11581 cggcatgccg caggtcatag aaggcaaaac ctgctacatg caacagccat tgccgtttgc
    11641 caaaaaaatg cgggaattcg tgacgcaata cggggtcagc attattggcg gctgctgcgg
    11701 tacgcggccc gaccatataa aagccatggt cgatgctctt ggcgacggcg tcaaacctgc
    11761 catgcgatgt ccggaatttc agccgtctct ttccagtgct tatcagagtg tcacgataga
    11821 gcagcagccg cggccgttca tgataggcga gcgggctaat gccaacggct cgaaaaattt
    11881 tcgcgaactt ctgctcgccg gtgatttcga cggcgcgttg caggtcggcc ttgaccagca
    11941 ggctaccggc gcgccgcacg cgctggacct ttgcgtagca tttgccggcc gtgatgagaa
    12001 ggcggacatg gaacggctga taaaaatgtt cgccaacagc atccgcctgc cggtagtgat
    12061 tgattcgact tctcccgacg taatagaaac ggcgctgaaa aattatcctg gcagggcgct
    12121 tataaattcc atcaaccttg aagacggcgg aactaatctg cgtaaaatct gtcgtcttgc
    12181 aaagaaatac ggcgcagcct gcatagccct gaccatcgat gaaaaaggca tggccatgac
    12241 ggcagacgca aaactcgcgg tggctacgcg gatatacgac attgccgtct gtgagtgcgg
    12301 cttgcggccg agcgacctgg ttttcgacgt tctgacgttt accatcggct caggcgacga
    12361 atccaccaaa aacgccggcg tagagacgct cgccggtata cggctggtca agcgcgacct
    12421 gttgggcgtt tttaccacgc ttggcctcag caatatatct tttggtttga agccggccag
    12481 ccgccgcata ctgaattcca tgttcctgca cgaagccatc tccgccggcc ttgataccgc
    12541 catagtggat gccgccagaa tcctgcccct gaatcagatg gatgcccggg atcgcgcggt
    12601 ggctctcgac ctgatatata atcgagtgag cgaaaaaaat cccgacccgt tggccagatt
    12661 catagaacgg ttcaacgacg ccactgttcg gcagcagacg caggagccgg ccaaagaccg
    12721 tcggccggct gaggagctgc ttaccgagca catgtttgcc ggcaaacgcg acggactgga
    12781 agacgtcatc gccgcactgc tggaaaagta tgcgccgacc gagataataa acaatcttct
    12841 gattgaggcc atgcgcata
//
LOCUS       DAONFI010000014        12274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_5757_length_12274_cov_4.218758, whole genome shotgun sequence.
ACCESSION   DAONFI010000014 DAONFI010000000
VERSION     DAONFI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_12274_cov_4.218758"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PLK08_00990"
     CDS             complement(<1..997)
                     /locus_tag="PLK08_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPS52103.1"
                     /translation="MLDTTVKFFSDFWTVLEQMSPYLLFGFILAGIIRVLLPVKWIER
                     HLGGEGFWASLKSSAIGVPLPVCSCGVIPLAASLRKHGAGAGPTTAFLISTPQTGVDS
                     ILATWAMLGPVFTIFRVAAALLSGLLGGWAVSLLVRTSTTDVKNHTTDNNHDDELPKT
                     VKGKISEAVRFAFVTLPGDLAGALMVGLIISGLISALIPDKFFAQHLSSLPLQMLLML
                     AMGIPMYVCSTASIPIAAALVLSAGISPGAALVFLMSGPATNAATITIITRMLGWRAM
                     ITYLASVAVTSIGAGILMDTAFSGIKVSPMAMHQHGQGVSIIYTIAAVILLAMLLI"
     gene            complement(1054..1722)
                     /locus_tag="PLK08_00995"
     CDS             complement(1054..1722)
                     /locus_tag="PLK08_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPS52104.1"
                     /translation="MNNNWDMSKYDVIFFDLDGTLTDPSMEMVSSAVYALQKCGIASP
                     DKAKLQKFLTEPLLECFEQHFGMTQEQANEAFNHYWQYYATQGISRNKPYNGVPELLD
                     KLSEQGKIMCVATARQTENAVGVLAATGLTRYFTHILGASNDMSRRTKKMIIFDLIAS
                     IGEHDMEKIIMVGDRTADILGAQANGIDTIGVTWGQDSQDEIKATNPTYMANSVKEMA
                     DLLL"
     gene            complement(1827..5348)
                     /locus_tag="PLK08_01000"
     CDS             complement(1827..5348)
                     /locus_tag="PLK08_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPS52105.1"
                     /translation="MLKILQKLFIKSIGGSRNDRIIRSCMNLVHEKINPLETMMQSLD
                     DNELRAASLDLRKQTLAGDVKRNDVLPQAFALIREASRRGRNHRQYDVQLVAGMILDR
                     GWIAEEATGEGKTIACYPAIYMAWLEGFKTHVVTVNDYLVERDANFAKPIFELLGVTV
                     GFIRSEMPAYGDGATARRAAYECDVTYGTNSEFGFDYLRDNMKMSPAEQVQGRLDFAI
                     VDEVDSILIDEARTPLIISGPAYGKTDRYNKADAVARELISRNRPYDQANRQVENLTM
                     RLQTLEGELKKNKKSDAADKLQKQIEQTRDELVQAEDARSRQTQYYEVVLDKKSAHMT
                     HEGVTAAQEIAGVGSFYVGANMEWPHLMEQALRAHLVYERDKDYVVQDGQIVIVDEFT
                     GRLLEGRQWSDGLHQAVEAKERVTVKEENQTLATITLQNYFKLYKKLAGMTGTAMTEA
                     EEFAKIYKLDVASVPTNVPCCRNDSEDRIYADEDAKFKAIVEEINEYSKTGRPVLVGT
                     TSIEKSEKLSQMLVHTYGIEHELLNGRPENAAREAEIVFKACQQHELSKKNSQMVGNV
                     TIATNMAGRGTDIKLSDGAKQSGGLHIVGTERHEARRIDNQLRGRAGRQGDPGSSRFF
                     LSLEDDLMSIFAGDWTKKMLAWLGLTGDMAIENKRISNGIERAQKKVEERNFEIRKNL
                     LEYDEIMDVQRHGFYSMRQAVLEGRNIQRLVFRMVNDSIASAAENFLDGSYPQRCIGE
                     WARNTLQAAVRDEHIKAKTADDMPSLEADLREAAKSEAYATIAITLGEYMDEELEASA
                     WDLRGLSSWAKSRFGVDISQNQLRKMNPHEVEQALCDSAAEKIDKIDLSPLLECLEPD
                     FAKKSFAGWANSKFNLGLSGDDFDGTAQQAVEKIMTGVHELYRRRQVEYPVEYAVEMT
                     VGRNGEDNIYALEELCRWANVKFGTDFTAENLQGKNAHELLKMLTDISHEWLEGDKLS
                     QCINGAAGIEPSVKDAIEFAKKRFYTELAEKDFEGCDVLEKLIEIGRLFLTRELAELE
                     RFVLLQIFDQSWKDHLLSMDHLKSAIGLRGYAEQDPKVAYKREGGKLFEEMFAGIREK
                     LSSMIFKVRLASREDELESVYQVSEQVHEQLHGYGHLAQGAGQAAQQPGESQKIKQIV
                     RQDVKVGRNDPCPCGSGKKYKKCCGRM"
     gene            5594..5962
                     /locus_tag="PLK08_01005"
     CDS             5594..5962
                     /locus_tag="PLK08_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52106.1"
                     /translation="MQANVSLQEVDYNEMEFGDLDRDQAEAAFRSIDWAPRVSVFEQT
                     SQKTDDCCPPSFLVTFGRRHYLSITVQPDRIIVQLSMPGNGSAKIKTFEKNYSTAAME
                     EMPTIIAKAFDDFGLLFDSI"
     gene            complement(5988..7043)
                     /locus_tag="PLK08_01010"
     CDS             complement(5988..7043)
                     /locus_tag="PLK08_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52107.1"
                     /translation="MKYKPDWAEARERLTALWNHETLDRPCIAVIAPNGKKTPAPELP
                     ANLEDIWLNIEWVKKAYLAKLENTWWGGEAIPSYLTMGGWVLCLGGTPKFAQDTIWFE
                     HVNIDFNGPSPFYYKDGDPWVEKYMALYNVIVEMAGKDDFLVGKPSNLPANDLFSMFM
                     GTEEFLFSLIDHPEWMRNAIIKGAQEQLKLCRKIEKNICEKHDFWYGHGGWMCFWAPQ
                     PFTSTQSDVSCMLSPEMFEEFIMPEMDILGQAYGALWYHLDGGNARQHLPRLLSLPYL
                     KVLQYVPAPCEPANGPEHLDFYRTIQAAGKIVHIGVPKENVLPLVKSLDTSLLMLDVT
                     CNSVEEGQALLKATKKA"
     gene            complement(7286..9823)
                     /gene="topA"
                     /locus_tag="PLK08_01015"
     CDS             complement(7286..9823)
                     /gene="topA"
                     /locus_tag="PLK08_01015"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPS52108.1"
                     /translation="MGKAVKDKKVDGKCLVIVESPAKAKTINKYLGSDFVVKASMGHV
                     RDLPNKNPKGVKAPVPGVDLEHDFEPTYEVLPRGKKTLAELKPLAKTAPLVYLATDLD
                     REGEAIAWHLAQSLKLNPENTRRVVFNEITRSAIKEAFANPRDVDMNMVDAQQARRIL
                     DRIVGYQVSPLLWKKVAGGLSAGRVQSVAVRLIVEREREIDAFMPEEFWRIGGIFTKN
                     ASDAPSIAAKWQKFLADTDEKGDGPTKKSQLEFLAQNHAFTAELVSFAGQKFNANNVE
                     QATRVARALGVSVKDTQTKEDPKAKGPARNKITVVASLAADKNFIVTDVSQKLTFSHP
                     GAPFITSTLQQAASTQIYFAARRTMSVAQQLYEGISIPGEGEVGLITYMRTDSRNISA
                     EAIAAVRNLIEDKFGPKYLPDKPNFYASGKMAQEAHEAIRPTDASRTPESIKSSLTDD
                     QFKLYRLIWQRFVASQMTSAQWNVTEAAITTDTSDGIAQFKAAGRSLAFDGYLRATGI
                     SSNGEQILPELAAHDRVAPVDISPTQHFTQGPSRYTEASLVKALEADNIGRPSTYAAI
                     IQTIQDRNYVVQENRAFRPTDLGFVVTDKLVAHFPEIFEVKFTAHMEDQLDAVEAARE
                     DWIAVLKEFYEPFSRQLVQATEDMVHAKAETQPSEYKCPQCGKPMVYRFGKNGRFLSC
                     SAYPDCKAAQPIDRDGKPVGTTHTDIACPKCGKPLILRKGRFGPFLSCEDYPKCDGIV
                     NLDRKGGVKLPSAPPLQIEVTCPKCNSPVMNLRRSKKGPWISCAAYPKCRGRVAWSKL
                     DTADQKKYEAALEKHEAQHPKITIRKTNGDEAEEGYTPQIIQEEIDS"
     gene            complement(9962..10489)
                     /locus_tag="PLK08_01020"
     CDS             complement(9962..10489)
                     /locus_tag="PLK08_01020"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HPS52109.1"
                     /translation="MLRAFKPVAAPDARVLVLGCMPGVKSLEMGQYYAHPHNAFWPIC
                     EKLFAASPQLPYKMRCDRLIAAGIALWDVLHSCRRKGSLDSNIEPDSMQPNDFAAFFR
                     KHRKIRAIFFNGTTPQKIFTKQILPTLNSQAAALPMTRLISTSPACAAWNFEQKLENW
                     RQILMILKSDEISNP"
     gene            10708..>12274
                     /locus_tag="PLK08_01025"
     CDS             10708..>12274
                     /locus_tag="PLK08_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPS52110.1"
                     /translation="MKCEGTEVFTGSELLVKGALETEGGVHLLGGYPGSPIAGYFDSL
                     TALKDVLREKGIRAVINNNEALAAAMLNGTQSLPLRSMIAMKSVGVHVAADALALANL
                     AGANPEGGAIVIYGDDPWSDSTQVASDSRYISKHLFIPTIEPSNPQEIKDFVNLSFKL
                     SAISELVVGYIITTNLADGGGTVECRPNHFPSVNMYDRIEMVTKDIDFDKRVLLPPRT
                     WWQEASLTGRFSRAMKAARELGLNRFEYRNETSVRKPLGFVCAGLGHDYLTQALYEMG
                     QLGQAPILKLGLSYPVDPEMIEDLARQVERIIVVEERRGFMEEQIAEIMTALRQKDAA
                     FDDVMIFGKKFPDGLKGLPEIRGLDPSIIIQTLGPLVCRYCTTADSMAASTIQTEIRT
                     IEQTESVKVTTKLPARLPTFCPGCPHRDSANLCTDIKRCFADPDYMRQKHHREPVDLI
                     FHGDIGCYTMLMFPPNNELMHDLSGMGLGGGTGSGIDPFTTNNKVTFMGDSTFFHIGM
                     TSISQAIKFNQNIT"
BASE COUNT         2648 a         3086 c         3230 g         3310 t
ORIGIN      
        1 gtatcagcaa cattgcaagc agaatgacgg cagcaatagt ataaattatg gaaacgccct
       61 ggccatgctg gtgcatcgcc atcggcgaaa cttttatacc actgaaagcg gtgtccatca
      121 gtatgcctgc gccgattgac gttacagcta cgctggccag atacgttatc attgcccgcc
      181 aacccaacat acgggttata atggtgattg tcgccgcgtt ggttgccggc cccgacatga
      241 ggaataccaa tgcggcgcca ggcgaaattc cggcgctgag tacaagcgcg gcggcgatgg
      301 gtatcgatgc cgtcgagcag acatacatgg gtatgcccat tgcaagcatg agcaacatct
      361 gtaacggtaa gcttgaaaga tgttgcgcaa aaaatttgtc gggaattagc gcgctgatta
      421 agccagaaat tatcaagccg accataagcg ctccggccag atcgcctggc aaggtgacga
      481 atgcaaatcg gacagcttct gagattttgc ctttgactgt tttcggtagt tcgtcatcat
      541 ggttattgtc agtagtgtgg tttttcacat ctgttgttga agtgcgtaca agcaggctga
      601 cggcccagcc gccgagcagg ccgctgagca gggctgcggc tacgcggaaa atcgtgaaca
      661 ccggcccgag catagcccac gtggcgagta tgctgtcaac tccggtctgc ggcgtcgaga
      721 tgagaaatgc agtcgtgggc cctgcacccg cgccgtgctt acgcaacgag gcggctaacg
      781 gtattacgcc gcaactgcac accggcagcg gaacgccgat ggctgatgac ttgagagatg
      841 cccaaaatcc ttcgccgccc aggtgtcttt ctatccactt tacaggaagc agcacgcgga
      901 ttatgccggc aagtataaac ccgaaaagga gatacggcga catctgttcc agaactgtcc
      961 agaaatccga aaagaatttt acagtcgtat caagcatggt aaatcctggc tgctttcaaa
     1021 aaatcgattg ttgaaagtca tcgctacgaa acattatagc agtaaatccg ccatttcctt
     1081 gacggaatta gccatgtacg tcggatttgt cgccttgatt tcgtcttgcg aatcctggcc
     1141 ccatgttacg ccgatagtgt ctatgccgtt ggcttgtgca ccgaggatgt cggcagtgcg
     1201 gtcgccgacc atgataattt tttccatgtc atgttcgccg atggatgcta tcaagtcgaa
     1261 tattatcatc tttttcgtac ggcgggacat gtcgttgctt gccccgagaa tgtgcgtaaa
     1321 atatctggtc aacccggtag cagccagaac cccgacggca ttctcggtct gacgcgccgt
     1381 cgcaacacac atgatttttc cctgttccga cagtttatcg agcagttccg gcacgccgtt
     1441 gtagggtttg ttgcggctaa tgccctgcgt cgcgtagtac tgccagtaat ggttaaatgc
     1501 ttcgttcgcc tgttcctgcg tcatgccgaa atgttgttca aaacattcca gcagcggctc
     1561 tgtcaggaat ttttggagtt ttgccttatc aggcgaggca atcccgcatt tctgcagcgc
     1621 ataaaccgca ctgctgacca tctccatgct cggatctgtc agtgtaccgt cgagatcaaa
     1681 aaatattaca tcgtatttcg acatatccca attattattc atggccacac cccctacaca
     1741 ataaaatata tcatattttt tgtttgaagc ggtgtcaacc cgttagcgag gttctgtaaa
     1801 ttattttcat tttgaacata cgtttattac attcggccac agcatttttt atattttttc
     1861 ccactgccgc acgggcatgg gtcgtttctg cccactttta cgtcttgccg tacaatctgt
     1921 tttatttttt gtgactcccc gggctgttga gccgcctggc cggcgccttg tgcaagatgg
     1981 ccgtaaccgt gtaattgctc gtgaacctgc tcggagacct gatatacgct ttccagttcg
     2041 tcttcacggc tggccagtcg aactttgaaa atcatgctgc tgagtttttc acgaatgccg
     2101 gcgaacattt cttcaaacag tttgccgcct tcgcgtttgt aggctacttt tgggtcctgt
     2161 tcggcgtaac cgcgtaagcc tattgcgctc ttcagatgat ccatgctaag cagatggtct
     2221 ttccatgact ggtcgaaaat ctgcaacagt acgaaacgtt ccagctcagc caattcgcgc
     2281 gtcaggaata atctgccgat ttctatgagt ttttccaaaa cgtcacagcc ttcgaaatcc
     2341 ttttccgcca attcggtata aaatcttttt ttggcgaatt cgatagcgtc tttgacggac
     2401 ggttcaatac cggcagcccc gtttatgcac tgtgaaagtt tatcgccttc gagccattca
     2461 tgcgaaatgt cggtcagcat tttcagcaat tcgtgtgcgt ttttgccctg aagattttct
     2521 gcggtgaagt cggttccgaa tttcacattt gcccatcggc agagttcttc cagtgcgtag
     2581 atgttatctt cgccgttgcg gccaacggtc atttcgacag catattcaac gggatattcc
     2641 acctgccgcc ggcgataaag ctcatgaacg ccggtcatga ttttttccac cgcctgttgt
     2701 gctgtgccgt cgaaatcatc gccggaaagg ccaaggttga attttgaatt tgcccagccg
     2761 gcgaacgatt tttttgcaaa atccggctca agacattcca gcagcggcga gaggtcgatt
     2821 ttgtcgattt tctcggcggc tgaatcgcat agcgcctgct ctacttcgtg aggattcatc
     2881 tttcgcaact gattttgcga gatgtcgaca ccgaagcggc ttttggccca actgctcagg
     2941 ccccttaaat cccacgccga cgcttcgagt tcttcgtcca tatattcgcc gagagttatg
     3001 gcgatggtgg cgtaggcttc tgatttggcg gcttcgcgga ggtcagcctc aagcgagggc
     3061 atatcgtcag ccgttttcgc ttttatatgc tcgtcacgca cggcagcctg gagagtattg
     3121 cgggcccatt cgccgatgca tcgctgggga taagagccgt ccaggaaatt ttcagccgcc
     3181 gacgcaatgg aatcgttgac cattcgaaaa acaagtctct ggatattcct gccttcgagc
     3241 accgcctgtc gcatgctgta aaacccatga cgttgcacat ccattatttc gtcgtattca
     3301 agcaggtttt tgcggatttc gaagttgcgt tcttcgactt tcttctgagc gcgttctatg
     3361 ccatttgaga tacgtttgtt ttcgatggcc atgtcgccgg tcagacccag ccacgcgagc
     3421 attttttttg tccagtcgcc tgcgaatatg ctcatcaggt cgtcttcgag gctgaggaag
     3481 aatctgcttg agccagggtc gccctgcctg ccggctcggc cgcgcagctg gttgtcgatg
     3541 cgccttgcct cgtgacgttc cgtgccgacg atatgcaggc cgccggactg ttttgcgccg
     3601 tcggaaagtt tgatgtccgt gccgcggcca gccatgttgg tggctatcgt gacgttgccg
     3661 accatttgcg agtttttctt gctcagttcg tgctgttggc aggctttgaa tactatttca
     3721 gcctcgcgag ccgcgttttc cggccggccg ttgagcagtt cgtgttcaat gccgtatgtg
     3781 tgtacgagca tttgtgagag tttttcactc ttctcgatac tggttgtgcc gacgaggacg
     3841 gggcggccgg tttttgaata ttcatttatc tcttcgacga tggctttgaa tttggcgtct
     3901 tcgtcggcgt agatgcggtc ttcggagtcg ttacggcagc agggtacgtt agtgggcacg
     3961 ctggctacgt cgagcttgta gattttggcg aattcttcgg cttcggtcat ggctgtgcca
     4021 gtcatgcctg cgagcttttt gtagagcttg aaataattct gcagcgtgat tgtcgctagc
     4081 gtctgatttt cttccttgac ggtgacgcgt tctttggctt cgactgcctg gtgaaggccg
     4141 tccgaccact gccggccctc cagcagtcgg ccggtaaatt catcgactat gactatctgg
     4201 ccgtcctgca cgacataatc cttgtcgcgt tcatagacca gatgagcgcg cagggcctgc
     4261 tccatgagat gcggccattc catgttggcg ccgacgtaaa agctgcccac gcctgcgatt
     4321 tcctgtgccg cggttacgcc ctcgtgcgtc atgtgagccg acttcttgtc cagcaccact
     4381 tcgtaatatt gagtttgccg gcttcgggcg tcttccgcct ggacgagttc gtcgcgggtc
     4441 tgttcaatct gtttctgcag tttgtcggcg gcgtcggatt ttttgttctt tttcagttcg
     4501 ccttcaagcg tctgaagtct cattgttagg ttttcgacct gtctgtttgc ctggtcatac
     4561 ggccgattac gggaaatcag ttcgcgtgcc acggcgtcag ccttgttgta gcggtcggtt
     4621 ttgccgtagg cggggccgga aataatcagc ggcgtacgag cctcgtctat caggatggag
     4681 tcaacctcgt cgacgatggc gaaatccagc cgtccctgta cctgttccgc aggcgacatc
     4741 ttcatgttgt cgcgcagata atcgaagccg aattcgctat ttgtgccgta tgtcacgtca
     4801 cattcgtacg ctgcccgccg cgcggttgcg ccgtcgccgt atgccggcat ttccgaacgt
     4861 atgaaaccaa ctgtaacgcc cagcagttcg aaaatgggtt ttgcaaaatt ggcgtcgcgt
     4921 tcgacaaggt agtcgttgac ggtgacgacg tgcgttttaa aaccttccag ccacgccatg
     4981 tagatggctg gataacatgc gatggtttta ccttcgccgg tggcttcttc ggcaatccag
     5041 ccgcggtcga gtatcatgcc ggctaccagt tgtacgtcat attggcgatg gttacggccg
     5101 cggcgggagg cttcgcggat aagggcgaac gcctgcggca gaacgtcgtt acgcttgacg
     5161 tcgccggcta gcgtctgttt tcgcaggtcg agcgaggcgg cgcgcagttc attatcgtcc
     5221 agggactgca tcatcgtttc cagaggattg attttttcat gcacaaggtt catgcatgaa
     5281 cggataatgc ggtcgttacg cgacccgccg atacttttta tgaacaattt ctgcaatatt
     5341 tttaacatct tgtttcctgt tcgtatggct gaatttataa cctcggaagt atcgcatttt
     5401 ttgacgaaat aatcaattaa tttcagatta agacgctgat ttgcggcaaa cagttccggc
     5461 gcagcgaggc tgattttttt gcgatgaatt tcaataaaat cgacctattt tgttttcaga
     5521 gccgccgcaa aatgttatta ttaacaatcc gacataagtc cgccggacgg gctggttttt
     5581 acggagacaa ataatgcagg caaatgtgag tttacaggaa gttgattata acgaaatgga
     5641 attcggcgat cttgatcgtg accaggctga ggcggcgttc cggtcgatag attgggcccc
     5701 cagggtgagt gttttcgagc agacatcgca gaagaccgac gactgttgtc cgccgagttt
     5761 tctggtgacg ttcggccgca ggcattatct gtccataacc gttcagcccg acagaattat
     5821 cgtgcaatta tccatgccgg gcaatggttc ggcgaaaatt aaaacgttcg aaaaaaacta
     5881 ttcgacagcg gcgatggaag aaatgccgac gattatcgca aaagcatttg acgattttgg
     5941 cctgctgttc gattcaattt aatccggcag tggcgggtcg ttatttttca ggcttttttg
     6001 gttgccttca gcaatgcctg tccttcttcc acactgttgc acgtcacatc cagcattaac
     6061 aaagatgtat ccagtgattt taccaatggg aggacatttt cttttggcac tccgatatga
     6121 actattttgc cggctgcctg tatagtacgg taaaaatcaa gatgttcagg gccgttagcc
     6181 ggttcgcatg gtgccggcac gtattgaaga actttcaaat aaggcagcga caacaatcgc
     6241 ggcaaatgct ggcgggcatt accgccgtcc agatgatacc acaacgcacc gtaggcctgc
     6301 ccaagtatat ccatttccgg cataatgaat tcttcaaaca tttccggcga gagcatgcac
     6361 gacacgtctg attgcgtgga tgtgaacggc tgaggagccc aaaagcacat ccatccgccg
     6421 tggccgtacc agaagtcatg tttttcgcaa atgttttttt caattttgcg acataatttc
     6481 aattgttcct gagcgccctt gatgattgca ttacgcatcc attccggatg gtcaatgagg
     6541 ctgaacaaaa attcttctgt tcccataaac atcgagaaaa ggtcattggc aggaagatta
     6601 gagggtttcc ctaccagaaa atcatctttg ccggccattt cgacaatgac gttatataag
     6661 gccatgtatt tttcaaccca cgggtcgcca tctttgtaat aaaatggaga agggccatta
     6721 aaatcaatat taacgtgctc aaaccatatt gtgtcttgtg caaattttgg cgtgccgccc
     6781 agacagagta cccatccgcc cattgtcagg tatgacggaa tagcctcgcc gccccaccat
     6841 gtgttttcga gctttgccaa ataagccttt tttacccatt caatgtttag ccatatgtct
     6901 tccagatttg ctggtaattc aggtgccggc gtttttttac cgtttggggc tattactgca
     6961 atgcagggtc ggtcaagcgt ttcgtgattc caaagggcgg ttaaccgttc acgggcttct
     7021 gcccagtcgg gcttatattt catgatgata aaaccttata ctatgtttag tttgaaacaa
     7081 catattatca cttatgaaat attaccacgg atattcgaga tgcatagagt aacatattgt
     7141 tttagcaggt aatttattgc tatagttgta taggagcaat attataagat tttcgaaagc
     7201 tgcgccgcca tcggaccgtt tttatgacgt agcagatgca taattgccgc tcatttgcgt
     7261 ctcacagctt aattgagaac accctctagc tgtcgatttc ttcctggatg atttgcggcg
     7321 tgtagccttc ttcggcttcg tcgccattag ttttgcgaat ggtaattttg gggtgctgcg
     7381 cttcatgttt ttccagggct gcttcatatt ttttctggtc agccgtatcc agtttgctcc
     7441 aggctacgcg gcctctgcat ttgggatacg ccgcacaact tatccacggc ccttttttgg
     7501 aacgccgcag gttcattacc ggcgaattgc acttgggaca tgtcacttct atttgaagcg
     7561 gcggagcgga gggcagtttt acgccgccct tgcggtcaag attaacaatg ccgtcgcatt
     7621 tgggataatc ttcgcagctc aggaacggcc cgaaacggcc tttgcgcaaa atcagcggct
     7681 taccgcattt cgggcacgcg atatcggtat gcgtcgtgcc gacgggtttg ccgtcgcgat
     7741 ctataggttg ggcggctttg cagtccggat acgcgctgca actgaggaat cggccgttct
     7801 tgccgaagcg gtataccatc ggtttgccgc attgagggca tttatattcg ctcggctggg
     7861 tttcagcctt ggcgtgcacc atatcttcgg tggcttggac gagttgccgg ctgaacggct
     7921 cgtaaaattc cttgagtacg gcaatccagt cttcgcgtgc cgcttcaacg gcgtctagct
     7981 ggtcttccat atgcgcggtg aatttcacct caaaaatttc ggggaaatgc gcgacgagct
     8041 tatccgtgac aacgaagccc aggtcagtcg gccggaaggc gcggttctcc tgcacgacat
     8101 aattcctgtc ctgtatggtc tggataattg cggcataagt actgggccgg ccgatattgt
     8161 cggcttccag agccttgacc agcgacgcct cggtatacct cgacgggcct tgcgtgaagt
     8221 gttgcgtcgg actaatgtct accggcgcta cgcggtcatg tgccgccagt tcgggcaaaa
     8281 tctgttcgcc gttgctgctg atgccggtgg cgcgcaaata gccgtcgaat gcgagcgaac
     8341 ggccggcggc tttgaattgt gcaataccgt ccgatgtgtc ggtggtgatg gcggcttcag
     8401 tgacgttcca ctgggctgat gtcatctggc tggctacgaa tcgctgccat ataaggcggt
     8461 aaagtttgaa ctggtcgtcg gtcaggctgg actttatgct ctccggtgtg cgggatgcgt
     8521 cggttggacg gattgcctcg tgggcttcct gggccatctt gcccgatgcg taaaagttcg
     8581 gtttgtccgg cagatatttg gggccgaatt tatcttctat aagattgcgc accgcggcga
     8641 ttgcctccgc cgatatgttt ctgctgtcgg tacgcatgta agtaatcagg ccgacttcgc
     8701 cctcgccggg aatagatatg ccttcgtaaa gctgctgcgc tacgctcatg gtacgacggg
     8761 cggcgaagta aatctgcgtg ctggctgcct gctgaagcgt ggacgttatg aacggcgctc
     8821 cggggtgcga aaacgtcagt ttctggctaa cgtccgtaac gatgaaattt ttatccgccg
     8881 ccagtgatgc gacgacggtt attttatttc tggccgggcc tttagccttg gggtcttcct
     8941 ttgtttgcgt atccttgacg gatacgccca gtgccctggc gacgcgggta gcctgctcga
     9001 cattattcgc attaaacttc tggccggcaa aacttacaag ctccgcagta aaagcgtgat
     9061 tctgagccag aaattcaagt tgcgactttt ttgtcgggcc atcgcctttt tcatcggtgt
     9121 cggctaaaaa tttttgccat ttggcggcaa tcgacggggc atcggatgcg ttttttgtga
     9181 aaattccgcc gattcgccag aattcttccg gcatgaaagc gtcaatttca cgctcccgtt
     9241 cgactatcaa ccgcacagcc accgactgta cgcgtccagc cgacaagcca ccggcgactt
     9301 ttttccacaa cagcggcgaa acctggtaac caactatgcg gtcgagaatt cgtcttgcct
     9361 gctgagcatc taccatgttc atatcgacgt cgcgcgggtt ggcgaaagcc tcttttatag
     9421 cggagcgggt aatttcatta aaaaccacgc gacgggtatt ttcggggtta agtttgagcg
     9481 actgcgcgag atgccaggcg atggcttctc cttcgcggtc aaggtcggtt gccagataga
     9541 ctagcggtgc ggttttagca agcggcttga gttcggcgag ggtctttttg ccgcggggca
     9601 gaacttcgta agtcggctca aaatcatgtt ccaggtcaac gcctggaacg ggagccttta
     9661 cgcctttggg gtttttattg ggcaggtcgc ggacgtggcc catggaggct tttacgacga
     9721 aatcgcttcc gaggtatttg tttatggttt ttgccttggc tggcgattcg actatcacca
     9781 gacacttgcc gtcaaccttc ttgtccttta ctgccttgcc catcgaacct cccaactcgc
     9841 aaaaagggtc agatttcaac ttgccaaagg acagaatcat tgaatggcag acggtgtttt
     9901 gtcaagcact ttttttcatg aatttttaag tttcgatttt aagtgcctgt atttaatggg
     9961 cttacggatt tgaaatttcg tcggatttca gaatcatcaa tatttggcgc caattttcaa
    10021 gtttttgttc gaaattccag gctgcgcagg ctgggctggt ggagataagg cgagtcatgg
    10081 gcagagcggc ggcttggctg ttaagcgtcg gcagaatctg ctttgtaaaa attttctgcg
    10141 gagttgtgcc gttgaagaaa atcgccctga ttttacggtg tttgcgaaaa aaagctgcaa
    10201 aatcattggg ctgcatcgaa tcaggttcaa tgttggagtc cagcgagcct ttgcggcggc
    10261 atgagtgcag tacgtcccac agggctatgc cggcggcgat taatctgtcg catcgcattt
    10321 tataaggtag ctgggggctg gcggcgaaaa gtttttcgca tatcggccaa aatgcgttgt
    10381 gcggatgggc gtaatattga cccatctcca gcgactttac gccaggcata cagccgagta
    10441 caagcactct cgcatcaggt gcagcaacag gtttgaacgc tcgcaacata agtgaatttc
    10501 aaaatatttt taaattctgg aaacgggcat atgcgaattt tcctgacttt ccgattcgtt
    10561 caaatcatga tatccgcagc ctgccggagt tgcaagtcgc gcattttccc aaaggtacgt
    10621 ttcgcggagt gttttttgaa aataggcatg taaagagggt cgccagttcg ttacaatatg
    10681 acgactatga agacagaagg aagattcatg aagtgcgagg gcacggaagt gttcactggc
    10741 agtgaattgc tcgtgaaagg cgcccttgag acggaaggcg gcgtacatct gctcggcggt
    10801 tatccaggtt cgccgatagc aggctatttc gactcgctga ctgcgttgaa ggatgttttg
    10861 cgtgaaaagg gcatacgtgc cgtaatcaat aacaacgaag cgctggcggc agccatgctc
    10921 aacggcacac aatcgctgcc gttgcgttcc atgatagcga tgaaaagcgt cggcgtgcac
    10981 gtagccgccg atgcccttgc ccttgccaac ctggctgggg ctaatccgga aggcggagct
    11041 atcgttattt acggcgacga cccgtggagc gattccaccc aggtcgccag cgacagccgc
    11101 tatatctcca agcacctgtt cattcccacc atcgagcctt ccaatccgca ggaaatcaag
    11161 gattttgtaa atctttcgtt caaactgtcg gctatcagtg aactggtagt gggttacatc
    11221 atcaccacga atcttgccga cggcggcggc actgtggagt gtcggcccaa ccatttcccg
    11281 tcggtcaaca tgtatgaccg tatcgagatg gttacgaaag acatagattt tgacaaacgc
    11341 gtacttctgc cgccgcggac gtggtggcag gaggcgtcgc tgaccggccg attcagccgc
    11401 gcgatgaaag ccgcccgtga attggggctg aaccgctttg aatatcgtaa tgaaacttcc
    11461 gtccgcaagc cgctgggctt cgtctgtgcc ggcttgggac atgattatct tacccaggcg
    11521 ctgtacgaaa tgggccagct cggccaggcg cctatactca aactcggcct gtcgtatccg
    11581 gtcgacccgg aaatgataga agacctggcg cggcaggtgg agcggataat tgttgttgag
    11641 gaacggcgcg gcttcatgga agagcagatt gctgaaatca tgacggcgtt gcgtcagaaa
    11701 gacgccgcgt ttgacgacgt gatgatattc ggcaaaaaat ttcccgacgg cctcaaaggc
    11761 ttgccggaaa tacgcggcct agacccgtcg ataatcatcc agacgctcgg cccgctagtc
    11821 tgtcgttatt gtacgacggc ggacagcatg gcagcgagca ctattcagac agaaatccgc
    11881 acaatcgaac agactgaaag cgtcaaggtc acgacaaaac tgccggctcg tctgccgacg
    11941 ttctgccctg gttgtccgca ccgcgattca gccaatctct gcacggatat caagcgctgt
    12001 tttgccgacc cggattatat gcgacagaag catcaccgtg agccggtgga tctgattttt
    12061 cacggcgata tcggctgcta taccatgctc atgtttccgc cgaataacga acttatgcat
    12121 gatttgtcgg gcatgggact tggcggtgga acgggcagcg gcattgaccc gtttaccacg
    12181 aacaacaaag tcacttttat gggcgacagc acgtttttcc atatcggcat gacgtccatc
    12241 agccaggcta tcaagttcaa tcagaacata acct
//
LOCUS       DAONFI010000015        11816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6062_length_11816_cov_4.184848, whole genome shotgun sequence.
ACCESSION   DAONFI010000015 DAONFI010000000
VERSION     DAONFI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6062_length_11816_cov_4.184848"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1161
                     /locus_tag="PLK08_01030"
     CDS             259..1161
                     /locus_tag="PLK08_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52111.1"
                     /translation="MRITRQGQIGNLLAGEITIAAFLIAIFTYFTFIAATPIQPAIVV
                     QTEHFRIHAATTEQANKIASLAENSLYRISTEILTYKSSITSKIDVFIWSCQHDFSKS
                     LAYDKTSGTGLSRLLLAEDGTILRRIDIIPPAPVNTVLPHEIAHLLLNELDLNRQKAG
                     LPSIPLALHEGIAILAEGKYHDDLIMKAAVKLCCPVSDKNAEAVGRLSNFEKLIAATN
                     YDGTGMEVCDFYAQSYSFVEYLHAELKPEGMNRLLNGLAAGMPFNDAIQLALGDTSAN
                     FKQNLVRKWYAHSNKNTEALIAIR"
     gene            complement(1291..3885)
                     /locus_tag="PLK08_01035"
     CDS             complement(1291..3885)
                     /locus_tag="PLK08_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52112.1"
                     /translation="MLYKRLLSLLSVAIIALASSAFAQDSVGDSRDVIKAQMLVVKGD
                     ESLRMNQPENAVNYYERALQLSPGNAAIQQKLNDAREMTATAGSSSSLLNDVMARRNV
                     AKQMASHDIQKAINRSMELMAQPKDVKDFETAEQAAKIAQNILDQNKGYFTSEEYRAK
                     AADINRQLTWIDSKKREWNAQRVKVQAAAITEQIKKREMESLSQRKKKVAELTIQVQT
                     LRNEGKYHKALQVLDQILEIDPQNFWAQDTKYYLDRRATLSETREAMDDLETESMKLS
                     TSVRKAEVPWYVLIRYPKDWAELTAGRKRYSADGNIDAEGDRQLLEKLEMNIAKLKCP
                     GESFEDVINFLREYSGANFYVNWRALEAAGIERTATVTLDLKNVSLRKALELLLSDVG
                     GGATELGYVMSSGVITISTQEELNKDKQTKVYQISDLVHRVPNFVGERVKVDAIGDAV
                     SSNSSSGGGSSGGGLFDDSDTNTAETGREEANMTKGEAINALIELIKSTIDPNNWRPE
                     GEIGSMSTFNGNLVITQTSDNHRRIERLIAKLRETKTIQVSVESRFIIVNTGFLSQIG
                     VDLDFYFNLNGSDSSNQVTYTGDGTQPGYSNNYTRSTSPSVYNKGWSPIGANQSSSTF
                     ANVLGQETNVPNGIGTVANEPAMSIAGTFLDDIQVDFLITATQAHQNTRNLTSPRVTL
                     HNGQRAYVSVQRTMAYISGIEWVVSENSAAPNPEISYVPTGSMLDVDVTVSHDRRYVT
                     MTLRPQVVNLIDMRSADVYSTNGGSSPIEMPEIELQDIQTTVTVPDGGTLLIGGQKLS
                     GEVEREMGVPVISKIPVINRAFTNKGKIRDEQTLLILIKPKIIILDESEQDKGTWEEE
                     NAYMSQNN"
     gene            complement(4231..5988)
                     /locus_tag="PLK08_01040"
     CDS             complement(4231..5988)
                     /locus_tag="PLK08_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52113.1"
                     /translation="MSLEVPAGIGGRTEEVAPDAVDSTILNTVKQIETAIQNAKAPEL
                     EMRNYVDTLKAQQNDFLQNDVLASVDFGSPKISGMIQRGKLLKATEQVDLAKVQNVMP
                     APAKPFDWTGLELRLVESAGTEENNSGTPSGTLAEEPVWRGVDCYPIAELDAAWRKAL
                     EKTVIVPQIVAVKCEVEFEELAADGHWLPVNDIQTFRLPVAAVGDANQPARAPVIPDY
                     VEAQDGTTNLQNVQQAISDMRNWMIEELQPEYYKIWSREGQISWQQHMPLEKISALFE
                     EKSSASTPAVPAAAATNRSRYNRNVMAAEMGMMGEMTPNQPTYASRQRNSEATDDGSI
                     PNINSQLEAGKLLAWFHAKNLKFGKKYRCRFRWLFVNPLLTYTKSVKPEDIKDATTVK
                     TVASPWSPWSDTVAVSRKMEFFLTGANDQRKEVTVTVFTEAMGQLVRARFNRIQAGQP
                     IGGESEVSVVNPLSGEREKMAMNFQTGAIVIELNFNRRFITSFGRVKKDAVEMVYLDV
                     NGNVQSRIRENDLLLPKYRSMTQEAQAGEPEVEKVVREEKERTSSGKTKKTTTKIVPI
                     EPQMPGEIMPNMPKTRKIK"
     gene            complement(6155..7729)
                     /locus_tag="PLK08_01045"
     CDS             complement(6155..7729)
                     /locus_tag="PLK08_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52114.1"
                     /translation="MKKNNNVFMIALAAVTVVVVGVLMWLGYSRNQAITEELMAQRQG
                     VTGKLKNLSRKPYVNQSTNMTNEKRVEMLLDNLSQVRSLNYEFNSRNFSVPMLDITGA
                     TPRPALPYDARVWGSRELAARYVDAYRRIMADLLARLNPTTPPDDAEVNMAAKQQAEM
                     LENMARIEGKSAESARRPAGLMNEYGVGMTNPNSMAGVSQDVATLAKEYALNNAILMK
                     SQGGYVYADNGSLFPMFIFGKIYSLADLPPEDLWLAQKSIWIQTDIINAIAEAVKDVF
                     DARKLPDSERNVLNSPIKQLTAINIEKLKSAGSGRGGAMYPGMAEPGYGGTAGGEAVK
                     SDTLTTYGANALFDVANYSFTVVMPTRYLPVLEKKLLKQNYHVILSEEILPVDSAGGS
                     AAVGEGMMAGFGMSGGASSVKAVNGLYNFGTEPVCRITITAQLLLVTSFTRGQWDSAH
                     NMWVSYPLMPVEVMKNMPQTALRPEDRDFIEQFERLGRGEQPVEGRPAIPWYRNDAWR
                     PAKSSEKDARTPGKVQ"
     gene            complement(7747..9903)
                     /gene="pilM"
                     /locus_tag="PLK08_01050"
     CDS             complement(7747..9903)
                     /gene="pilM"
                     /locus_tag="PLK08_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPS52115.1"
                     /translation="MAASKTVWGIDIGQCALKAVKLQQVGERFQVDACEIIEHASVLS
                     QPDVDSRALIQTSLDEFLQRHDVTGSVVSVAVLGQSSFTRFVKLPPVEYKKIPEIVRF
                     EAEQQIPFPINEVTWRYQTFQDPDSPEIEAGIFALKKVDVSEMLSFFAPAGLEVDVLQ
                     MAPLALYNFMKVDGQYTSDGATLLADVGADKTHLVIVDGGKIWTRTIKIGGNNFTHAL
                     CKSFNLNFEKAEKLKRTAAASKYARQIFQVMRPVFANLVQEIQRSIGYYTSINRECRF
                     KKLLGLGCGFLLPGMQKYLEQNLNMHVEQFNTFNSADSNDPNFQARALSFGVAYGLAL
                     QGLNAGEVDCNLLPEEILKRRLWSKKKPWFVAAAAAVMVAVGLYAMRGFADYNTLNNV
                     KSQHALGEAQSIVKKLDDWKRESQKIDTNYEQKEEQIAQNLRFRAFSNIWPTINANIS
                     AALEQTMFGPNDAKILDAFALGTPQDQAALVGQIQSDQAVLELVGLLPFEQPSDDSTK
                     TATASLRSQLVKKFMSKPRKQRQLLLLAALKSMYVSDLDSVRTGTSSTSAGERGMMMG
                     PGGPGMAGEPGFNNGNQSARNEKAVRGLIVDLELRTPMDKEAADMAQKFKVNLKNTFD
                     ANPDLEVHGQIEVKYLTQQTEAGGNVAAGGRSGGFGAGNFEMGGKAATTAKGEDMPPV
                     LVHENDMTDVIIQVRVKLAVVGDGLPKPAKDEENNK"
     gene            10211..11638
                     /locus_tag="PLK08_01055"
     CDS             10211..11638
                     /locus_tag="PLK08_01055"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPS52116.1"
                     /translation="MKTFCINTLGCKVNQYESAALQAMLERSGLHQSGSGKADLVIIN
                     SCCITAAAARKSRQAIRKLARQSPEAAVAVVGCLSTYHESQVQKIFKDLGRPAEKILI
                     VGHHDDVADRLLMFLRGQTAAKTPQSAPKTPENIKKRRLDAIKAAPLATQGLAAIKDF
                     PEHQRAFVKIQDGCDAMCSYCVVPFTRSRVWSRGVDEILAECRNLISAGYREIVLCGV
                     FIGAFGRQTAIRRNWPATAATGGSPLAELLYKVSKLDGLWRVRLGSLEPLDVTDELLG
                     LAADNEKIAPHFHMALQSGSAKILRLMNRQYTPDEFLNAVSRLRQRLGDLPAITTDII
                     VGFPGEDDDDFNQTLAVAKLAGFSKIHAFPFSAIEPTPAWHRRREAPPPEIVRERMER
                     LADVEKTLADEYRCAFMGRTLEALAENSAGPAGYEYRAMTDRYLTVAFNSNSQINAGQ
                     LVTLQINGQQDDYLTAEKVSSPAVR"
BASE COUNT         2774 a         3140 c         2771 g         3131 t
ORIGIN      
        1 cagcatcata atccgttcgc aaagcagttt tattgctgtt ttactcaaaa tttcatgttt
       61 ttttacaaat ttcttgtcca gccatgaatt tgtaacgtct tgtcagtaaa aatcttatct
      121 caatacaaaa tcccaacctt cttcgaaaat aaaatagggt atttttttaa gaaaagtagg
      181 gggtttgcct gatataatat atatgtaatg ccttacatgg ctttcgagtt aatactgtgt
      241 agaatgtgag ggcatcgcat gaggattaca agacaaggac aaattggcaa tctcctggct
      301 ggcgagataa caatagccgc ttttctaatc gctattttta catactttac ttttatcgct
      361 gccacaccaa tccagcctgc tatagtagtt cagaccgaac attttcgcat tcatgccgca
      421 acaactgagc aagccaacaa aatagcttct ctggcagaaa acagtttata tcgaatcagt
      481 accgagattc tcacatacaa atcttccata acctcaaaaa ttgacgtatt catctggagc
      541 tgccagcatg atttcagtaa gtccctcgct tacgataaaa catcaggaac cggcctcagc
      601 cgtctgcttc tcgctgaaga cggtacaata ttgagaagaa tcgacatcat tccgccggca
      661 cctgttaata ccgttctgcc gcatgaaatc gcacacttgc tcctgaatga acttgatttg
      721 aacagacaga aagccggctt gccatccatc ccgttagccc tccatgaagg catcgcaata
      781 ctggcggaag gtaaatatca cgacgacttg ataatgaaag cagcagtcaa actgtgttgc
      841 ccggtatcag ataaaaacgc agaagcggtt ggccgtcttt ccaattttga aaaattgata
      901 gccgcaacga attatgatgg tacaggcatg gaagtttgcg atttttatgc gcaaagctac
      961 agttttgttg aatatctgca tgccgaactc aagccggaag gcatgaacag gctactcaac
     1021 ggccttgcag caggcatgcc ttttaacgat gcaatacaac tggctctcgg cgatacatct
     1081 gcaaatttca agcagaatct cgttcggaaa tggtatgccc actccaataa aaacacagaa
     1141 gcattaatcg cgatacgata aaataacgca tcaacattga tagacttctc tgcaaaatat
     1201 cgaacaaacc agctatcatg atgaagctct aaataaaaaa ggcggaagtt ttttagcaaa
     1261 actcccgcct tttgataatt tggtttattt tcagttattc tgactcatat atgcgttttc
     1321 ttcttcccag gtacctttgt cctgttcaga ctcatcaagt atgataattt tcggcttgat
     1381 gagaataagc agggtctgct catcacgtat tttacctttg ttggtgaacg ccctgttaat
     1441 tacgggtatc ttgctgataa ccggcacgcc catttcacgt tcaacttcgc ccgaaagttt
     1501 ctggccgcca atcaacaatg taccgccgtc cggaacggta accgtcgtct gaatatcctg
     1561 aagttcaatt tcaggcattt caatcgggct tgaaccgccg ttggtactgt atacgtcggc
     1621 actacgcatg tctataagat tgacgacctg cggcctaagt gtcatggtta catagcggcg
     1681 atcatgcgaa accgtcacat caacgtcaag catcgaacct gtaggaacat atgaaatttc
     1741 cggattcggt gcggcagagt tttcagaaac gacccactct atgccggata tgtatgccat
     1801 cgttcgctga acgctgacat aggcccgctg gccattgtgc aacgttacgc gaggcgatgt
     1861 caaattacgc gtgttctgat gagcctgagt ggctgttatc aggaagtcaa cctgaatatc
     1921 atcaaggaac gtacctgcaa tggacatcgc cggctcattc gcgaccgtac caataccgtt
     1981 gggcacattt gtctcctgcc ccaaaacgtt cgcaaaagtc gatgaactct ggttagcacc
     2041 tatcggcgac catcctttgt tataaacgct tggcgaagtg cttctggtat agttgttgct
     2101 gtagcctggc tgagttccgt cacccgtata agtaacctgg ttactactgt cactgccatt
     2161 gagattgaaa tagaaatcaa ggtcaacgcc aatctggctg aggaagccgg tgttgactat
     2221 gataaagcgg ctttctacag aaacctgaat ggttttggtt tcacgaagtt ttgctatcaa
     2281 cctttcaata cggcgatgat tgtcagaagt ctgcgtaatc accagattgc cgttaaaagt
     2341 gctcatggaa cctatttcgc cttcaggccg ccaattattg gggtcaatgg tgctttttat
     2401 aagttcgata agggcattta ttgcctcacc cttggtcata ttagcttctt cacggccagt
     2461 ttcggcagtg ttggtatcac tatcgtcaaa aagcccgccg ccgctactgc cgccgccact
     2521 gctggaattg ctggaaacgg catcgccgat ggcatcgacc ttgacacgtt cgccgacaaa
     2581 attcggcacg cgatgcacaa ggtcacttat ctgataaact ttggtttgct tgtccttgtt
     2641 aagttcttcc tgcgtcgata ttgttataac gccggaactc attacataac caagttcggt
     2701 cgcaccgccg ccgacatcgc tcagcagaag ctcaagagcc ttgcgaagcg aaacattttt
     2761 caggtcaagc gtaactgtag cagttcgttc aataccggct gcttccaaag cgcgccagtt
     2821 gacataaaaa ttcgcgccgg aatattcacg caaaaagttt atgacgtctt caaaactttc
     2881 gccaggacat ttgagcttcg caatattcat ctccagcttt tcgagcaact gacggtcacc
     2941 ttcggcatca atattaccgt cggcagagta gcgtttacgg ccggcagtca gttcagccca
     3001 atccttggga tatcttataa gtacgtacca ggggacctcg gctttgcgga cagaggtcga
     3061 aagtttcatg ctttccgttt ccagatcgtc catcgcttcg cgggtttccg aaagcgtcgc
     3121 tcttctgtcc agataatact tggtgtcctg agcccagaaa ttctgcgggt ctatttccaa
     3181 tatctggtca agcacctgga gagctttgtg atatttgcct tcgtttctga gagtctgaac
     3241 ctggatggtc aactctgcaa cttttttctt gcgctgcgat aaagattcca tttcgcgttt
     3301 ctttatctgt tcggtaatag ctgccgcctg aaccttcacc cgctgggcat tccattcccg
     3361 ttttttggaa tctatccagg taagctgccg gttgatatcg gcggcttttg cgcgatactc
     3421 ttcggacgtg aaatagcctt tgttctggtc gagaatgttt tgtgctattt tggcagcctg
     3481 ctcagctgtt tcaaaatcct tgacgtcctt gggctgagcc atcagttcca tggaacgatt
     3541 gatagccttc tgtatatcat ggctagccat ctgcttggcg acattccggc gagccattac
     3601 atcgttcaga agcgaagaac tgctgccggc agtcgcggtc atttcacgcg catcgttgag
     3661 tttttgttga atcgcggcat tgccaggcga aagctgaagt gcccgctcat agtagttcac
     3721 ggcgttttcg ggctggttca tgcgaagcga ttcgtcgccc ttcacgacaa gcatctgcgc
     3781 tttaatgacg tcacgcgaat caccaacact atcctgcgca aacgcagaac tcgccagagc
     3841 tataatcgcc acactcagca gcgaaagaag acgtttgtac agcaccgcta ttctcctttc
     3901 tgagttaaaa aacccagatt acaagttatc cgtaaggaca acacatatta caacaggctt
     3961 gcgggtacaa agaccggctg cccgaactct ttacgcctta tatcgacaaa atttatctct
     4021 tcgctttatc ggattttaca aaaatttata gcgtaggaaa ttacagtatc ggcggctttt
     4081 aagcaagatt tttttggcat aacattaaaa aatcatcact taaaggcttc tatcggtcat
     4141 tttcaccata ataatcgccg tttagttcaa tccgataaaa tatttaaggc aatcacctta
     4201 cttgagtatt atttccgctt attttaaaaa ttattttatt ttcctggttt tcggcatatt
     4261 gggcattatt tcgcctggca tttgcggctc tattggaact attttcgttg ttgttttttt
     4321 ggttttgccg ctggaagtgc gttctttttc ttcgcggacg actttttcca cttccggttc
     4381 gccagcctgc gcttcctgag tcattgaacg atacttcggc agcagcaggt cgttttctcg
     4441 aatacggctt tgaacgttgc cgttcacatc gagatatacc atttccaccg catctttctt
     4501 gacacggccg aatgaagtta taaaccgtct gttgaagttc agttctatga caatcgctcc
     4561 cgtctggaag ttcatggcca ttttttctct ttcgccggag agtgggttga ctacactcac
     4621 ttccgattcg ccgccgatag gttggccagc ctgtatcctg ttgaacctgg cccgaacgag
     4681 ttgtcccatt gcctcggtaa agaccgtcac ggtcacttcc ttgcgttgat cgttggcgcc
     4741 tgtgaggaag aattccattt ttctgctcac cgctacagtg tcagaccacg gcgaccacgg
     4801 cgaagcaacc gttttgactg ttgtggcatc cttgatatcc tcaggtttga cggatttggt
     4861 ataggtaagc agcggattga cgaacagcca gcggaaacgg cagcggtatt ttttgccgaa
     4921 tttcagattt ttcgcatgga accacgccag aagtttgcct gcttcaagct gtgaattgat
     4981 attcggtatt gaaccgtcgt cagtcgcttc actgttgcgt tgccggctag cataagtcgg
     5041 ctgattggga gtcatttcgc ccatcatccc catctcagcc gccattacgt tcctattgta
     5101 tctgcttcta ttcgtagccg cggctgcggg tacggctggc gtactggctg acgatttttc
     5161 ttcgaaaagt gcggatattt tttcgagcgg catatgctgt tgccaggata tctggccttc
     5221 gcggctccaa atcttataat attccggctg aagctcctct atcatccagt ttcgcatatc
     5281 ggaaatcgcc tgctgcacgt tttgcaaatt tgtagtgccg tcctgggctt cgacataatc
     5341 aggaatgaca ggcgcacgag ccggctgatt agcatcgcca accgcggcaa ccggcagacg
     5401 gaacgtttgt atgtcgttca ccggcagcca atggccgtcg gcggcaagtt cctcaaattc
     5461 gacctcgcat ttcacggcta ctatctgcgg aacaataacg gttttttcca atgcctttcg
     5521 ccatgccgcg tcaagctcgg caatcgggta gcagtctacg ccgcgccaaa ccggctcttc
     5581 cgccagagtg ccggagggcg ttccgctgtt attttcttcc gtaccggcac tttccaccag
     5641 ccgaagttca aggcccgtcc agtcaaacgg tttagcaggc gcgggcatga cgttctgcac
     5701 ttttgccagg tcgacctgtt cggtggcttt gagcagtttg ccccgctgaa tcatgccgct
     5761 tattttcgga gacccgaaat ccaccgacgc cagaacgtca ttctgcagaa aatcgttctg
     5821 ctgtgccttg agcgtatcca cgtaattccg catttcaagt tcaggagctt tggcattctg
     5881 tatggcggtt tctatctgtt tgaccgtatt cagaattgtc gaatcgacgg catcgggagc
     5941 aacttcttcc gttcggccgc ctatgccggc tgggacctcc aaactcatcg gagtcgacag
     6001 accgtaatac atgacggcaa acaacaatgc aatcacacac aaaagaaaaa tcactttttc
     6061 tatatgtttg tcaaaaaatc ctggtccttg gaatctggcc atgttctcag gcctcctatc
     6121 ttgtgtttat tacaatcatc aactactgcg gactctactg cacttttcca ggagtcctgg
     6181 catctttttc agacgacttt gccggccgcc atgcgtcatt tctgtaccac ggtatcgccg
     6241 gccggccttc aacgggctgt tcgcccctgc ccagccgttc aaactgttca atgaaatcac
     6301 ggtcttccgg ccgcaaagcg gtctgcggca tatttttcat cacttccaca ggcatgaggg
     6361 ggtatgacac ccacatattg tgcgccgaat cccattggcc tctggtaaag ctggtcacca
     6421 gaagcagctg agccgttatc gttatacggc agactggctc agtaccgaaa ttgtacaacc
     6481 cgtttactgc cttgacggac gaagctccgc cggacatgcc aaagccggcc atcatgcctt
     6541 cgccaactgc cgccgaacca cccgccgaat caaccggcag aatttcctcg ctcagaatca
     6601 cgtgataatt ctgcttgagc agctttttct ccaaaacggg cagatatctt gtcggcataa
     6661 ctacggtaaa gctgtaattc gcgacgtcaa aaagcgcatt cgcaccatat gtggtgagtg
     6721 tgtcggattt tacggcttcg ccgccggctg taccgccgta gccaggttca gccatgccag
     6781 gatacatcgc accaccacgg cctgaaccgg ctgatttcaa tttctcaatg tttatcgccg
     6841 tcagctgttt gatgggcgaa ttaagaacgt tgcgttcgga atccggaagt ttcctggcgt
     6901 caaagacatc cttgaccgcc tcggcgatgg cattgattat gtcggtttga atccatatgc
     6961 ttttctgggc cagccataaa tcttccggcg gcaggtcagc cagagaatat attttgccga
     7021 aaatgaacat cggaaacagc gacccgttgt cggcatacac gtagccgcct tgtgatttca
     7081 tcaatatggc gttgtttaaa gcatactctt tagccaacgt agctacatcc tgtgaaacgc
     7141 cggccatgct gttcgggttt gtcattccga cgccatactc gttcatcaag ccggccggcc
     7201 ttcgggcgga ctccgccgac ttgccttcga ttctggccat gttttcaagc atttcagcct
     7261 gctgcttggc agccatattc acctccgcat cgtccggagg cgtcgtcgga ttaagccttg
     7321 cgagcaaatc ggccattatc ctgcgatacg catccacata cctggcggct aactccctgc
     7381 tgccccagac ccttgcatca tacgggagag ccggccgagg cgtcgcgccg gtaatatcaa
     7441 gcataggcac tgaaaaatta cggctgttga actcataatt gaggctgcga acctggctga
     7501 gattgtccag cagcatttca acgcgctttt cgttcgtcat gttggtgctt tgattgacgt
     7561 agggcttgcg tgagagattt ttcagctttc ccgtcacgcc ctgccgctgc gccatgagtt
     7621 cttccgtgat agcctggttc ctggaatagc ccagccacat gagaaccccc acgactacaa
     7681 cagtcaccgc cgcaagcgct atcatgaaca cgttattatt tttcttcatc acacactcca
     7741 gtggtgttac ttattgtttt cttcatcttt cgccggcttt ggcaagccgt ctccgacgac
     7801 agccagcttc acgcgaacct gtatgatgac gtcagtcatg tcgttttcat gcaccaggac
     7861 gggcggcata tcttcccctt tggcagtcgt cgccgctttg ccgcccattt cgaaattgcc
     7921 ggcgccgaat ccgcctgacc tgccgccggc tgcgacattg ccgcctgcct ccgtctgctg
     7981 cgtaagatac ttgacttcta tctggccgtg aacttccagg tcgggattcg cgtcgaatgt
     8041 gttcttgaga ttgaccttga acttctgagc catatccgca gcctctttat ccatgggagt
     8101 gcgcaattcc aaatcgacaa tcaggccgcg gactgctttt tcatttctag ccgactgatt
     8161 gccgttatta aagcccggct cgccagccat gcccggcccg ccaggcccca tcatcatgcc
     8221 gcgttcgccg gcagacgtcg aagacgttcc cgtcctaacc gaatcaaggt cgcttacata
     8281 catactttta agcgccgcaa gtaacagcaa ctgccgctgt ttgcgaggct tgctcatgaa
     8341 tttcttcacc agctgcgaac gaagcgatgc cgtggcagtt ttcgtcgaat cgtccgacgg
     8401 ctgttcgaaa ggcagcaggc ctacaagttc cagaactgcc tggtcgctct gtatctggcc
     8461 gacaagcgca gcctgatcct gcggcgtacc caatgcgaaa gcatccagta ttttcgcatc
     8521 gttaggcccg aacatggtct gttcgagagc ggcggatatg ttggcattta tagtcggcca
     8581 gatatttgaa aatgcgcgaa aacgaaggtt ttgggcaatc tgctcttctt tctgttcgta
     8641 attggtgtct atcttctggg attcgcgttt ccagtcgtca agtttcttga cgatgctctg
     8701 cgcttcgccg agggcatgct gacttttaac attattgagg gtattgtaat cggcaaagcc
     8761 gcgcatcgca taaaggccga cagccaccat aactgcggcg gcggcggcta cgaaccacgg
     8821 cttttttttg ctccaaaggc ggcgtttgag aatttcttcc ggcaggagat tgcaatcgac
     8881 ttcgccggcg ttcaggccct gcagggccaa cccgtacgcc acgccgaacg ataacgccct
     8941 ggcctgaaaa ttcgggtcgt tcgaatccgc actgttgaaa gtgttaaact gttcaacgtg
     9001 catgttgaga ttctgttcca gatatttctg catgcccggc agaaggaatc cgcagcctag
     9061 ccccagaagc tttttgaagc ggcattcacg gtttatggat gtgtaatatc ctatcgatcg
     9121 ctggatttcc tgaacgagat tcgcgaaaac cggccgcatc acctggaata tctgccgcgc
     9181 gtacttgctg gcggctgcgg tgcgtttgag cttttcagcc ttctcgaagt tcaggttgaa
     9241 acttttgcat agtgcgtgcg tgaagttgtt gccgcctatc tttatggttc gcgtccagat
     9301 tttcccgccg tcgacgatta ccaggtgcgt cttgtccgcc cccacgtcag ccagcagcgt
     9361 agcgccgtcc gacgtatatt ggccgtccac tttcatgaaa ttgtatagcg cgagcggagc
     9421 catctgaagc acgtccactt ccagccctgc cggcgcaaaa aacgaaagca tctcggagac
     9481 gtcgactttt ttgagtgcga atatgcccgc ttctatttcc ggcgaatcgg ggtcctggaa
     9541 cgtctggtat cgccacgtga cctcgttgat tggaaacggt atctgctgct cagcctcgaa
     9601 gcggacgatt tcgggaattt ttttgtattc caccggcggc agttttacga accgcgtgaa
     9661 gctgctttgt ccaagaacgg cgacggacac gactgagccg gtgacatcgt gtcgttggag
     9721 aaattcatcc agagacgtct ggataagagc cctggaatcg acatcaggct gcgacaggac
     9781 acttgcgtgt tcgatgattt cacaggcatc tacctgaaaa cgttcaccga cctgttgcag
     9841 cttgaccgcc tttagagcgc actgacctat gtcaatgccc cataccgttt tcgatgctgc
     9901 cataggtact ccttggctta ctacatttac gggacacttc cctgcaacag cccaaatgta
     9961 cctgccacat cggccaacgt attactgtta caggcaaata taccttgaac acattatttg
    10021 cagacggctg gcgtattttt caggctgcgc cgcgtccggt ttttataatt gtttattaat
    10081 atattatgtc gcattcgcca aagcaacaag agtttatcga gtatctttat ataatttccc
    10141 cacttgcagt tcaaggccat tgcaaaaaaa cctgttaaaa tgacacatgt gatgatataa
    10201 ccgccctgcg atgaaaactt tttgcataaa tacgctcggc tgtaaggtaa atcagtatga
    10261 atccgccgcg ttgcaggcga tgcttgagcg ttctggactg catcaatcgg gcagcggcaa
    10321 ggcagacctg gtgataataa actcctgctg catcaccgcc gcggcagccc gaaaaagccg
    10381 ccaggctatc aggaaattag cccggcagtc acccgaagca gccgtcgccg tcgtcggctg
    10441 cctcagtaca tatcacgaat cgcaggtaca aaagattttc aaagatttgg gccggccggc
    10501 tgagaaaatc ctcatcgtcg gccatcatga cgacgtagcc gacagacttc tgatgttcct
    10561 tcgcggccaa acagctgcaa aaacacccca atccgcccca aaaacgccgg aaaacataaa
    10621 aaaacggcgg ctggacgcca tcaaagctgc cccactagcg acacagggcc tggcggcgat
    10681 aaaagatttt cccgaacatc agcgtgcgtt cgtgaagata caggacggct gcgacgccat
    10741 gtgcagctac tgcgtcgtgc cgtttacgcg gtcaagagtc tggtctcgcg gagtcgatga
    10801 aattctggcg gaatgtcgca atctcatttc ggcgggttat cgggaaatcg tactctgcgg
    10861 cgtatttata ggggcattcg gccggcagac agccatccgt cgtaactggc ctgccacagc
    10921 ggcaaccggc ggctcgccgc tggcggaact gctatataag gtatcgaagc tggacggctt
    10981 atggcgggtt cgcctcggca gcctcgaacc gctggacgtg accgacgaat tgctgggact
    11041 ggcggctgac aatgaaaaaa tcgcgccgca ttttcacatg gcactgcaga gcgggtcggc
    11101 taaaattctc agactgatga accgccagta tacgcccgac gaattcttga acgcagtcag
    11161 ccggctcaga cagagactcg gcgacctgcc cgcgataaca accgacataa tcgtcggttt
    11221 tcctggcgaa gatgatgacg acttcaatca aacgctcgcc gtcgcgaaac tagccggatt
    11281 ttccaaaatc cacgcatttc ccttcagcgc aatagagcct actcccgcct ggcatcgccg
    11341 ccgcgaagcc cccccgccgg aaatcgtgcg tgaacgaatg gaacgactcg ccgacgtgga
    11401 aaaaacgctg gctgatgaat accgctgcgc cttcatgggc agaacgctcg aagcattggc
    11461 ggaaaactcc gccggcccgg caggctacga atatcgggct atgacggaca gatatctgac
    11521 cgtagcgttc aacagcaact cgcaaataaa tgccggccaa ctcgtaacgt tgcaaatcaa
    11581 cggccaacaa gacgattatc ttaccgccga aaaagtttca tcgccggctg ttcgctgacg
    11641 acaaataaaa cagaaatgat gctttgaacg gcacatatta tttccatgcg aatttttttt
    11701 tcttataatg gcggcatgac atttaaacgg tcactatttt ttttcgtcat ttttttttcg
    11761 gccatgtcgt gtggagttgt ccgcggcgac acggaaacgg accgcccggg caaaac
//
LOCUS       DAONFI010000016        11812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6069_length_11812_cov_3.314366, whole genome shotgun sequence.
ACCESSION   DAONFI010000016 DAONFI010000000
VERSION     DAONFI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11812
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6069_length_11812_cov_3.314366"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLK08_01060"
     CDS             <1..316
                     /locus_tag="PLK08_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS52117.1"
                     /translation="ENAARLHPILMEGCRRVQAASNGKIGHVSGCGLVAAMQFTKPGT
                     TEPDAESAWKMVKAAYESGLMLFAPVGVGGCCIKVNPPLTINEEALREGLNVLEDIAA
                     NV"
     gene            433..1674
                     /locus_tag="PLK08_01065"
     CDS             433..1674
                     /locus_tag="PLK08_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPS52118.1"
                     /translation="MAGNGDKKTAMWVLTQLRAAGYQALFAGGCVRDMLLNLPSNDFD
                     VATDATPQQVQAVFPKVLMVGAKFGVAMVIKSARTVEVATFRNDGSYSDGRRPDGVLF
                     SSAREDALRRDFTINGMFYDPLADEVIDYVGGKADLKKGIIRTIGQPSRRFGEDYLRM
                     LRAVRFAVRFDFKIEKQTTAAIRKLCSNIVKISGERVCDELARMLVGPNPVKAMRLLE
                     KLGLAREILPEFFADGDEPWQRAVERIEKISQLDEIGMIGAMLCEMPVSEEQKIIRRW
                     GASNLWRKTAEFMIVNRDSWRDVLDGRLATFKKLLANPAWRSLFELWRTREIIETGGN
                     EKCCRISAAANDIDPAQISPRPLVSGSDLGKKGITPGREMGAILSELYDLQLDGVIKS
                     RADALREAMKIYDKNKRATHF"
     gene            complement(1661..3931)
                     /locus_tag="PLK08_01070"
     CDS             complement(1661..3931)
                     /locus_tag="PLK08_01070"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPS52119.1"
                     /translation="MIFIRRYSQTDIMLTERRRMERPLLWLVWISALMFSMVDGNFFY
                     MGLLTLVTAMSYFATMRNMEICLDRWLVNFAAIAATVWIIAEHKIMGQRTIVSICHYT
                     ILIQICKIFERKRPFDYILTQLLAMILVVSSAMLCAFFWLAAMVVGYVFLACYVAMVF
                     TLKRGLDRAAEHTLPGEYEHIDPKKLAWNSIRNWPGKPLRQVMFSLGCMSLFMGCLAF
                     MLAPRTAKGILAVINSPEPLESMQEKTMSLGQSREIYLSRRQIMSVQIITNRHIGPAQ
                     SFSSQYLRENVLTKYSQSSWQTEDPGFMHINIPQIQNSRDYANLGIIVHRISLMPGFQ
                     GGLPAPYPTLSIEMPDSSKISVTRNLVYNDDDENTFTRRAYETYSFGEPMTQKQINIV
                     MKFTNWSHTPRRPAAKINMSDNDKKLIIELAETWAGDLLRQRDALPEASRETMNIEIA
                     SRLAAKLDERCSYTLDLRDANPNRDAVVDFLFYMKKGHCEYFASTLGVMCCLLDVPAR
                     VAIGYHFDDYKRSSKSYIVRECDAHAWCEVYSNETNWTIVDPTPASAMASPNSDLQFW
                     AKLKAKWMSLQYWWYENIIGFDATSQEHMKQDIQKTSENFFRHIGDIAAKLWNSIVLL
                     ISKGVIDAFLIKAFIWFLIVSLSLFVLKICQIIFFNMLMARTPEGQARMRRLKVLRHI
                     VDITERKGLPYRPENTLREWSAQAIEQFNLPAAEMRQIVELHNRWRWGNIKPSPEEME
                     KILAAARIIENRIKSA"
     gene            complement(3928..5106)
                     /locus_tag="PLK08_01075"
     CDS             complement(3928..5106)
                     /locus_tag="PLK08_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPS52120.1"
                     /translation="MVKIGKARKMLVHLHATWGGWIFIAASLVVGWAATRNNAPLLFV
                     IFGTMIALITFSGVHAKNMLEGIELQRELPQRVWQYETLYFGYVLKNMRRSPAMSISM
                     REIGLTGVKDASALCAYLPANGVFRSGSRLTALHRGRTDLVAVRITSSFPFGLVRLKK
                     EFWQTNSMIVWPAKGKLKANLLRHGAAMTSTARPSRMQSGQDEFFGLRDYRIGDNLRW
                     IHWRKSANRQTPAVREMSHPVPDVLFIIVDIQQTNGDEIFAGDQEKMLRFTATLMDDA
                     LGHGYQVGMAFAGVNRVEIINPGYGVGIRARLLDSLADMTEPENCTLHDVIKALPMTP
                     LRKSQTLLITQSIARLPSQPMTHLAEFCRHLSVISSEQIEQVFEDSPVVSIYVGRKKK
                     "
     gene            complement(5126..6067)
                     /locus_tag="PLK08_01080"
     CDS             complement(5126..6067)
                     /locus_tag="PLK08_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPS52121.1"
                     /translation="MHVDYKRKLAQLMGQIETVLYGKREVVREVIIALLAKGHVLLED
                     VPGVGKTTLSRAIAGSIDATFSRIQLTPDMLPSDMLGVSIYESTTGQFSFKKGPIFAN
                     IVLADEINRTTPRTQSALLEAMNESQVTADGKTYRLGPPFMVIATQNPYEFEGTYFLP
                     ESQLDRFMIRLRIGHPNRSAERQILLNRPSQTVLGEIQPIMSVDELLYIQNMVDEVSV
                     DDSILNYIIDIAEATRRAEELTVGLSPRGSLALLQAAKSAALLDDRQFVIPDDVKNLA
                     PSVCAHRLLARSFAHDGAASAAEAVFIRILETVPTPQ"
     gene            complement(6331..6906)
                     /gene="folE"
                     /locus_tag="PLK08_01085"
     CDS             complement(6331..6906)
                     /gene="folE"
                     /locus_tag="PLK08_01085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPS52122.1"
                     /translation="MTEEQTNAIKQAVVTILKAVGEDPTREGLKGTPARVAAMYDELF
                     SGLREDPAKHLDVYFTEQYDEMVILRDIPFYSMCEHHLLPFMGKAHVAYLPGGKVVGL
                     SKLARVVEAFARRPQVQERLTGQIADLLMDHIGARGAGVIVEANHTCMTIRGIKKPGS
                     VMVTSAMRGLFRSNLATRTEAINLLTGGRTL"
     gene            complement(6996..7682)
                     /locus_tag="PLK08_01090"
     CDS             complement(6996..7682)
                     /locus_tag="PLK08_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPS52123.1"
                     /translation="MEKFALIGLGRFGTRLAKLLSQNGAEVIAVDKRRDLVEEIRDFV
                     SRAVCLDATDEESLRSQGIDKVDVAIVGVGTAFESAVLTTVLLKQLGVPRVISRATSQ
                     VRSQVLSRVGADDIVNPEQESADRWCSRLMATKVIEKIELADDFSLVQAPAPEKTVGK
                     TLGELDIRKNFKIQVVAIKRTVEDIDADGVKRTRQFVISVPMAETTIRKGDVLMLIGS
                     DDAVRIFAEE"
     gene            complement(7759..9666)
                     /locus_tag="PLK08_01095"
     CDS             complement(7759..9666)
                     /locus_tag="PLK08_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPS52124.1"
                     /translation="MIPPRTTTRLGNRVLPVVLNILLAMAGMVAVFTLVLQYGGFSYD
                     SLPLSRDTLINIQMVVVLVFILDRFLRLMISRSRLEYLKDNWLDFTLIFLLIVAGLVI
                     YRYKGDLLSAGVLYILVTQIYLLAVLILRVISAHYLIAGSGLPPSWLVIGSFMSLWLV
                     GSGLLMLPAAVRQEYWTSWNYLDALFTSVSATCVTGLVVVDTGTHFTFFGQAVILFLI
                     QLGGLGIMIFGTMLAILAGRALTIRSSEALSEMLASDERGNMARMVKFVVSSTFIIEI
                     IGAICLYSLFSQPTVLDASGQRLTFAGTIWYSVFHSISAFCNAGFALYGQNLMQGTNG
                     GWAVPLREHWQILGVVAPLIILGGLGFPVLGNLFEAARDRIRRRKQKNAISLKEDPKK
                     NLYIHTKIVLVTSAVLVILGAVGFSLVETGVVKPKYPDLMHSPSGRNLSSGEWASMNG
                     IQRLRESFFQSVTARTAGFNTMDMGSLSTAGKLWMCGLMTIGGSPASTAGGMKTITVA
                     LLFISAWCILKQRNSVEVFGRTIADELFRRAATLALLYIFMLAVVTFMLCMAMPAEQF
                     IDLLFEACSACGTVGLSTGVTSRLTVFSKIVVILGMFIGRIGPLTLIIALGAHVRKAN
                     YSYPVENVIIG"
     gene            complement(9855..10259)
                     /gene="hisI"
                     /locus_tag="PLK08_01100"
     CDS             complement(9855..10259)
                     /gene="hisI"
                     /locus_tag="PLK08_01100"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPS52125.1"
                     /translation="MNFENLKKTISELKYSDDGLIPAVCVDCETKEVLMVAYMNNASL
                     EDTVRTGKTHFWSRSRQKYWMKGESSGHTQEVRAVYTDCDKDTLVIEVKQNGAACHEG
                     YRSCFFRKLNENGQWDIQGKRLFDPEKVYGKK"
     gene            complement(10314..>11178)
                     /locus_tag="PLK08_01105"
     CDS             complement(10314..>11178)
                     /locus_tag="PLK08_01105"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPS52126.1"
                     /translation="EGFPKKSGKVRDVYDIGEHLLLVATDRISAYDVIMTNGIPDKGR
                     VLTQISAFWFNLLKDVVPNHVVTTEFSELPGSLKKHTELDGRFMLCKKAKVVPIECVV
                     RGYLAGSGWKEYQKSRTVCGVKLPDGLLQCSELPQPIYTPSTKAEQGHDENISFERAC
                     ELQGSQTVTRLRDLSIAFYKKGREYAAKRGILLADTKFEFGLDSKGELMIVDEVLTPD
                     SSRFWPADKYQPGRDQESFDKQFVRNYLDEIKFNRQPPGPVLPADVVKNTREKYMEAF
                     TRLTGMPFKWA"
     assembly_gap    11179..11188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11364..>11812)
                     /locus_tag="PLK08_01110"
     CDS             complement(11364..>11812)
                     /locus_tag="PLK08_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPS52127.1"
                     /translation="TKFNNVMIDDATDKGICVIDLDTVMPGAAMYDFGDSVRIGAATA
                     VEDERDLDKVSMSLELFDRLTHGYLSAAKDFLTPRELELLAFSAKLITFEIGLRFLAD
                     HLSGDVYFKVHRENHNLDRCRTQFKMVQDMENKFDKMQEIVKKYAK"
BASE COUNT         2616 a         3058 c         3111 g         3017 t
ORIGIN      
        1 agaaaatgcc gccaggctgc atcctattct gatggaaggc tgccgtcgcg tgcaggctgc
       61 gtccaatggc aaaatcggcc atgtttcagg ctgcggactg gtggctgcca tgcagtttac
      121 caagcccggc acgactgaac ctgacgcgga atcggcatgg aaaatggtaa aagccgccta
      181 tgaaagcgga ctgatgctct ttgctcctgt cggcgtcggc ggatgctgca taaaggtcaa
      241 tccgccgctc acgataaatg aggaagccct tcgcgaaggt ttgaatgtgc tcgaagacat
      301 cgccgcaaat gtgtaatttt ttcgcggcat gctaaaaaaa aggcggattc acattgagtc
      361 cgcctttttc ttttttgttt gtcgacagcc tgaaagtgtc gccatcgacg caaaattgag
      421 tataatgcac gcatggctgg aaatggcgac aagaagacgg cgatgtgggt tttgacgcag
      481 ctgagagcgg ccggttatca ggcgctcttt gccggcggtt gcgtgcgcga catgcttctg
      541 aatcttccca gcaatgattt cgatgtcgca accgatgcca cgccgcagca ggttcaagcc
      601 gttttcccaa aagtattgat ggtgggggct aaatttggcg tggcgatggt tataaagtcg
      661 gctcgtaccg ttgaagttgc gacgtttcgc aatgacggca gttactccga cggccgacgg
      721 cccgacggcg tactattctc ctcggcacgc gaagacgctc tacgccgcga cttcaccata
      781 aacggcatgt tttacgaccc gttggctgac gaggtcatag attacgtcgg cgggaaggca
      841 gaccttaaaa agggtatcat tcgcactatc ggccagccgt ccaggcgttt cggcgaagat
      901 tatctgcgaa tgcttcgcgc cgtgcggttt gccgtacgat tcgatttcaa aatcgaaaaa
      961 cagacaactg ccgcgattcg gaaactttgc agtaatatcg taaaaatcag cggcgaacgg
     1021 gtctgcgacg aacttgccag aatgttagtg ggccccaatc cggtaaaagc gatgcggctg
     1081 cttgaaaaac tggggctggc acgcgaaata cttccggaat tctttgccga cggtgacgaa
     1141 ccgtggcagc gagccgtcga acggattgaa aaaatttccc aactcgatga aataggcatg
     1201 ataggtgcaa tgttgtgcga gatgccggta tccgaggaac aaaaaattat acgcaggtgg
     1261 ggcgcgtcga atctgtggcg aaagactgcg gaattcatga ttgtcaaccg tgatagctgg
     1321 cgggacgtac ttgatggccg cctggcgacg tttaaaaaac ttttggcaaa tccggcgtgg
     1381 agaagcctct ttgagctttg gcgaacaagg gaaataatcg aaaccggcgg caatgaaaaa
     1441 tgttgcagaa tatcagccgc tgctaatgat atcgaccctg cacagatatc gcccagacct
     1501 ttggtgtcgg gcagtgattt gggtaaaaaa ggaataacgc ctggcaggga gatgggagcg
     1561 attctgagcg aactttacga cttgcagctt gacggagtca taaagtcacg agcagacgct
     1621 cttcgggagg cgatgaaaat ttacgacaaa aacaaacgtg ctacgcactt ttgatgcgat
     1681 tctcgataat tcgggcggct gcgaggattt tttccatttc ttccggcgac ggtttaatat
     1741 tgccccatcg ccaacggtta tgcagttcca ctatctgtcg catttccgcg gcaggcaggt
     1801 tgaattgttc tattgcctgt gcgctccatt ctcgcaaagt gttttccggc cgatacggca
     1861 atcctttccg ctcggttata tcgacgatat gacgcaacac tttcaggcgg cgcattctcg
     1921 cctggccttc aggcgttcgg gccatgagca tgttgaaaaa gattatctgg catatcttca
     1981 ggacaaaaag gctaagcgaa actatcaaaa accatatgaa cgcttttatc agaaacgcat
     2041 cgattacgcc cttggagatt agcaggacga tactgttcca taattttgcg gcgatgtcgc
     2101 ctatgtgccg gaaaaagttt tccgatgttt tctggatatc ctgtttcatg tgctcctgcg
     2161 acgtggcgtc aaagccgatg atgttttcat accaccagta ttgcagcgac atccactttg
     2221 ccttcagttt ggcccagaat tgtaaatccg aatttggcga cgccatcgca gatgccggcg
     2281 tggggtctac aatcgtccag ttcgtttcgt tgctgtagac ttcgcaccat gcgtgagcgt
     2341 cgcattcgcg gactatgtat gatttggacg agcgtttgta atcgtcgaaa tggtaaccga
     2401 ttgccacgcg ggccggcacg tcaagcagac agcacatgac gccgagcgtg gatgcgaaat
     2461 actcacaatg gccttttttc atgtaaaaaa gaaaatccac cacggcgtca cgattcggat
     2521 ttgcatcgcg caggtcgagc gtatatgagc atctttcatc aagtttggcg gcgagtctgg
     2581 atgctatttc gatgttcatg gtttcgcggc tcgcttccgg cagggcgtcc cgctgcctga
     2641 gcaggtcgcc ggcccacgtt tccgccagtt cgattatgag ttttttatcg ttgtcgctca
     2701 tgttgatttt ggcggctggc cggcgaggcg tatgcgacca gtttgtaaat ttcatgacga
     2761 tatttatctg cttttgcgtc atgggctcgc cgaaggagta tgtttcgtaa gctcttctgg
     2821 tgaaggtgtt ttcatcgtcg tcgttatata ccagatttcg tgtgactgaa atttttgagc
     2881 tatccggcat ctctatggaa agagtgggat acggtgccgg caggccgccc tggaatcctg
     2941 gcatgagcga tattctatgt acgataatgc ccagatttgc gtagtccctg ctgttttgaa
     3001 tctgcgggat atttatgtgc atgaatcccg ggtcttccgt ctgccagctg gattgggaat
     3061 atttagtaag cacgttttcc ctcagatact gacttgagaa ggattgtgcc ggcccgatat
     3121 gcctgttggt tataatctgg actgacatta tctgacgcct ggaaagatat atctcgcggg
     3181 attggcccag agacatggtt ttttcctgca tcgactccag cggttctggc gaatttatta
     3241 cggcgagtat ccctttagcc gttcgcggtg cgagcataaa cgccagacat cccatgaaca
     3301 gcgacatgca gccgagtgaa aacattacct gccgcaacgg cttgccgggc cagttgcgga
     3361 tggagttcca cgcgagtttt ttggggtcta tgtgttcata ttcgccaggc agagtatgtt
     3421 ccgccgcgcg gtccagtccc cgcttgagag tgaacaccat tgccacgtaa cacgccagaa
     3481 agacgtatcc gaccaccatc gccgccagcc agaaaaacgc gcaaagcatc gccgatgaga
     3541 ccaccagtat catcgcgagt aattgcgtga gtatataatc aaatggccgt ttacgctcaa
     3601 aaatcttgca tatctgtatc aatattgtgt agtggcagat ggatacgatg gttctctgac
     3661 ccattatttt gtgttcggca attatccata cggttgccgc aatggcagcg aaattgacta
     3721 accatctatc aagacatatt tccatattcc gcatggtcgc aaaatagctc atcgccgtaa
     3781 ccagcgtcag caggcccatg taaaagaagt tcccatcaac catgctgaac atgagggcgg
     3841 atatccacac cagccatagc aggggccttt ccatgcgacg gcgctccgtc aacatgatgt
     3901 cggtctgtga atatcgtcgg atgaaaatca tttcttcttt ctcccgacgt aaattgacac
     3961 gactggtgaa tcctcgaata cctgttcaat ctgttccgac gaaatgacgc tcaaatgccg
     4021 gcagaactcc gccagatgcg tcatgggctg actgggaagc cttgctatgc tttgggtgat
     4081 tagcagcgtc tgcgattttc tcagcggagt catcggcagg gctttgatga cgtcgtgaag
     4141 cgtgcaattt tccggttcgg tcatgtcagc cagcgaatcg agcagcctgg cgcgaatccc
     4201 gacgccgtac cccggattga ttatctcaac cctgttgact cccgcgaatg ccatgccgac
     4261 ctggtatccg tgacccaacg cgtcgtccat gagcgtagcc gtgaatcgca acattttttc
     4321 ctggtcgccg gcgaatattt catcgccgtt ggtttgctgg atgtcgacga ttataaaaag
     4381 gacgtccggg acgggatggc tcatttcccg gacagccgga gtctgccggt ttgcactttt
     4441 tcgccaatgt atccatcgca gattatcgcc aattctgtaa tcccgcaggc cgaagaattc
     4501 atcctggccg ctctgcattc gactgggccg ggccgttgaa gtcatcgccg ctccgtggcg
     4561 gagaagatta gcctttagct tgcccttagc cggccagact atcatggagt tggtctgcca
     4621 gaattctttt ttgagccgca ccagcccgaa cgggaacgat gacgttatgc ggactgccac
     4681 gagatcagtc ctgcctctgt gtaatgccgt cagccgactg cctgagcgga aaacgccgtt
     4741 agccggcaga taagcgcaca acgcactggc atccttcacg ccggttaaac caatttcccg
     4801 catggatatc gacatagccg gactgcgacg catgtttttg agtacgtatc cgaaataaag
     4861 cgtctcgtac tgccatacgc gctgcggcaa ttcccgctgg agttctatcc cttcgagcat
     4921 gttttttgca tgtacgccgg aaaaggtaat cagtgctatc atcgtgccga atatcacgaa
     4981 aagcagcggg gcgttatttc gcgtggctgc ccagccgact accagcgagg cggcgataaa
     5041 aatccatccg ccccacgtgg catggagatg cacaagcatt tttctcgctt tgcctatttt
     5101 taccatgcga ctttaccgga actttttatt ggggagttgg aactgtttca agtatgcgga
     5161 tgaataccgc ctctgccgcc gatgccgctc cgtcgtgtgc gaacgacctc gccagcagcc
     5221 tgtgagcaca tacgctcggc gccagatttt taacgtcgtc tggtatgacg aactgtcggt
     5281 cgtcgagcag ggcggctgac ttggctgcct gtagcagggc tagcgagccg cgcggactga
     5341 gcccgacggt aagttcctcg gcgcggcggg tcgcttcggc gatgtcgatg atatagttta
     5401 aaatcgaatc gtcgaccgaa acttcatcca ccatattctg tatataaaga agttcatcga
     5461 cgctcattat cggctgaatt tcgcccaaaa cggtctgaga cggccggtta agcagtatct
     5521 ggcgttcagc gctcctgttg ggatggccga tgcgcaggcg tatcatgaaa cggtcaagct
     5581 ggctttccgg cagaaaatac gtaccctcga attcatacgg gttctgcgtg gcgatgacca
     5641 tgaacggcgg gccgagccgg tacgttttcc cgtcagccgt cacctgcgac tcgttcatcg
     5701 cttccagaag cgcgctttgg gttcttggcg ttgtgcggtt gatttcatcg gctaacacta
     5761 tgttggcgaa aatcgggcct tttttgaaag aaaactggcc ggtggtcgat tcgtatatgc
     5821 ttacgccgag catgtcgctc ggcagcatgt cgggcgtgag ctgaatgcgg ctgaacgtgg
     5881 cgtcgatact gccggcgatt gcccgcgaaa gcgtagtctt gccgacgcct ggtacgtcct
     5941 ccagcaatac gtgtcccttc gcgagaagtg cgattatgac ttcccgcaca acctcccgtt
     6001 tgccgtaaag cacagtctct atctgtccca taagctgggc gagcttacgt ttataatcta
     6061 catgcatttt gtatccctgt tacgattgtc atctaatatg ataaatcagt ataccaaaac
     6121 actttggatt gcacaagaaa tgctctggat gcggaaatta tttgtgcgaa agcatatctt
     6181 ctgtaaaacg caatagtatg ccggaaattg cgtattttcc ccgtttgacg gtattttttg
     6241 ggcaattcac ggacgccgga catgctctag gcgttccgcg attttcaaaa ctacgtcact
     6301 tggcgttgct ctttgcgaga atcgtcttga ttaaagcgtt ctgccgccag tcagcaggtt
     6361 tatcgcttcc gttcgcgtag ccaggttgct gcggaacagg ccgcgcatgg ctgacgtcac
     6421 cattaccgag ccgggctttt ttatgccgcg tattgtcatg catgtgtggt tagcttccac
     6481 tattacgcct gccccgcgtg cgcctatatg atccataagc aggtcggcta tctggccggt
     6541 aagccgttcc tgtacctgcg gacgacgggc gaacgcctca accacgcgag ccagcttgct
     6601 cagccccacc accttgccgc ccggcagata cgccacgtgt gccttgccca tgaacggcag
     6661 aagatgatgc tcgcacattg agtaaaacgg tatatcgcgc agaatgacca tttcgtcata
     6721 ttgctcggta aaatagacgt caagatgttt tgcggggtct tcccgcaagc cggaaaagag
     6781 ttcgtcgtac atcgccgcaa cccgtgcagg cgtacccttg aggccttcgc gcgtggggtc
     6841 ttctccgacg gctttgagaa ttgtaactac agcctgtttt atcgcgttcg tctgttcttc
     6901 agtcattttt ttacaccaac aatcattaaa aatagtaacc ggcagctttt acggcggctg
     6961 cgttcatctg cattttttgt tcttttttaa aaaaatcatt cttcggcaaa aattcgtaca
     7021 gcatcgtccg agccgatgag cataagcacg tcgcctttcc gaatggtggt ttccgccatc
     7081 ggcacggaaa tgacgaattg ccgcgtacgt ttgacgccgt ccgcgtcgat atcttccacc
     7141 gtacgtttta tggctacgac ctgtattttg aaatttttgc gtatatccag ttcacccagc
     7201 gttttgccca cggttttttc cggcgccggt gcctgcacga ggctgaaatc gtcggcgagt
     7261 tctatttttt cgatgacctt cgtcgccatg agacggctgc accatcggtc ggcggattcc
     7321 tgttcaggat tgacgatatc atctgcgccg acgcgggaaa ggacttgcga gcgtacctgg
     7381 ctggttgcgc ggctgatgac ccgtggtacg ccgagctgtt ttagcaggac ggttgtgaga
     7441 actgcgcttt caaaggctgt gccgacgccc actattgcca cgtcgacctt gtcgataccc
     7501 tggctgcgca gcgattcttc gtcagtggcg tcgaggcaga ctgcacggct gacgaagtcg
     7561 cgtatttctt ccacgagatc gcggcgtttg tcgacggcga tgacttctgc gccgttctgc
     7621 gagagcagtt ttgccagcct tgtgccgaaa cggcccaatc ctataagtgc gaatttttcc
     7681 atgtctgcca ctcccgtatt ttgttgcggg ccggtttacc cgcgtttttt tttttaaaag
     7741 ttttttgaaa aaagaagttt agcctattat aacgttttcc accgggtatg aatagttcgc
     7801 tttccgcacg tgcgcgccga gtgcgataat caacgtcaat ggccctattc tgccgatgaa
     7861 catgccaagt atgaccacta tttttgaaaa aaccgtcagc cgcgaagtca ctcccgtgct
     7921 cagtcctacc gtaccgcacg cgctgcatgc ctcgaaaagc aggtctatga actgttctgc
     7981 cggcatggcc atgcagagca tgaacgtcac cacggcgagc atgaaaatat acagcagggc
     8041 tagagtcgcc gcgcggcgga agagttcgtc ggcgatagtt cggccgaaca cttcgacgct
     8101 gttacgctgc ttcaggatgc accacgccga tatgaacaac aatgccacgg ttatggtttt
     8161 catgccgcct gccgtactcg ccggactgcc gccgatggtc atcaatccgc acatccacaa
     8221 tttgcccgca gtgctcagtg agcccatgtc catagtgttg aatccggcgg tgcgcgcggt
     8281 tacggactgg aagaagcttt ctctcagccg ttgaatgcca ttcatgctgg cccattcgcc
     8341 ggatgacaga tttctgcccg acggactgtg catcaggtcg ggatatttgg gtttgaccac
     8401 gcctgtttcc accaggctga aacctaccgc gccgagaatt acgagtactg ccgacgttac
     8461 cagcactatt ttcgtatgta tgtaaagatt ttttttaggg tcttctttca atgatatcgc
     8521 gtttttctgt ttacggcggc ggatacggtc tctggctgct tcaaacaggt tgcccagcac
     8581 cggaaaaccg aggccgccga ggattatcag cggggctacc acgcccagta tctgccagtg
     8641 ttctcgcagc ggtacagccc agccgccgtt cgtgccctgc atcagattct ggccgtaaag
     8701 ggcgaaccct gcgttgcaga acgccgaaat gctatgaaaa acgctgtacc atatcgtgcc
     8761 ggcgaacgtc agccgttgcc cggacgcgtc aagcacggtt ggctgggaaa acagggagta
     8821 aagacatatt gcgccgatta tctcgatgat gaatgtcgaa gaaaccacga acttgaccat
     8881 gcgagccatg ttgccgcgtt cgtcggatgc gagcatttcg ctgagagcct cgctgctgcg
     8941 aatagtcagc gcgcggccgg cgagtatggc cagcatcgtg ccgaaaatca ttatgcccaa
     9001 tccgccgagc tgaatcagaa acagaatgac cgcctgtccg aaaaatgtga aatgcgtgcc
     9061 ggtgtcaacc acgaccaggc cggttacgca cgttgcgctt accgatgtga acagggcgtc
     9121 aagatagttc cagcttgtcc agtattcctg tcgcacggcg gcaggcagca tcagcaggcc
     9181 tgagccgacc agccaaaggc tcatgaaact tcctattacc agccagctcg gcggcaggcc
     9241 gctgccggcg atgaggtagt gcgcgcttat gacgcgcaat atcagaaccg ccagcagata
     9301 tatctgcgtt accagtatgt aaagaacgcc ggctgacaga aggtcgccct tgtagcgata
     9361 tatcacgagg ccggcgacaa tcagcagaaa tatcagcgtg aaatccagcc agttgtcctt
     9421 caggtactca aggcggctgc gggatatcat cagccgcaga aaccggtcta atatgaatac
     9481 cagcacgacg accatctgta tgtttatgag ggtatcgcgg ctgagcggga ggctgtcata
     9541 gctgaagcct ccatactgca acacgagcgt aaagacagct accatgcctg ccattgccag
     9601 caggatgttc agaactacgg gcagtaccct gttgcccagc cgtgttgtcg tccggggagg
     9661 tatcatttat catttaccca aaccgacgct tgatttcgag tatcgttcgt caaagactca
     9721 gggcgtctga caaaatgccg ctgtagccgt cacgttgaat taaacctatc ccaaaaatat
     9781 gggcgtcgtc gccaggctgc aaaagaaccg attgcaggca gccaaagttt tttggatagg
     9841 ttcttaaaaa aactctattt tttgccgtaa actttttccg ggtcgaaaag acgcttgccc
     9901 tgtatgtccc attggccgtt ctcgtttagt ttgcggaaaa aacaggacct gtagccttcg
     9961 tggcaggctg cgccgttctg cttgacctca ataacgagcg tgtccttgtc gcagtcagta
    10021 tagacggcgc ggacttcctg agtatggccg gaactttcgc ctttcatcca gtatttctgg
    10081 cggctgcgcg accagaaatg cgttttgccg gtgcggacgg tgtcttcaag gctggcgttg
    10141 ttcatatatg cgaccatgag cacttccttc gtttcgcagt ccacgcatac cgcagggata
    10201 aggccgtcgt cggaatattt cagttcgctt atcgtcttct tgaggttctc aaaattcatt
    10261 gcagctcctt atttcagttg tcaaaaattc gtaatttttc aaaaaactct attttatgcc
    10321 catttgaaag gcatgccggt cagtcgcgtg aatgcttcca tgtatttttc gcgagtgttt
    10381 ttcaccacgt cagccggcag aacggggccg ggcggctgac ggttgaattt gatttcgtcc
    10441 agataatttc gcacgaactg cttatcgaaa ctttcctggt cgcggcccgg ctggtattta
    10501 tccgccggcc agaatcgcga cgagtccggc gtgaggactt cgtctacaat catgagttcg
    10561 ccttttgaat cgaggccgaa ctcgaatttc gtgtccgcca ggagtatgcc gcgcttggcc
    10621 gcgtattcac ggcctttttt ataaaaagct atcgacaggt cacgcaatct ggttacggtt
    10681 tgactgccct gaagctcgca ggcacgttcg aacgaaatgt tttcgtcgtg gccctgttcc
    10741 gcctttgtcg aaggggtgta aatgggctgc ggcagttcgc tgcattgcaa aaggccatcc
    10801 ggcagtttta cgccgcacac cgttcgcgac ttctgatatt ccttccagcc gctgccggcg
    10861 agataaccgc gaacgacgca ttctatgggt acgactttgg cttttttgca tagcatgaat
    10921 cggccgtcaa gctcggtatg ttttttcagg ctgccgggca gttcggaaaa ttcggtcgtt
    10981 acgacatgat tcggcacgac gtctttgagc agattgaacc agaatgcgct tatctgcgtc
    11041 agtacgcggc ctttgtccgg tatgccgtta gtcataatca cgtcatatgc gcttatgcgg
    11101 tctgtagcga cgagcagcag atgctcgccg atatcgtata cgtcacggac tttccctgat
    11161 ttttttggaa aaccctcgnn nnnnnnnngt cgtcagtaat gcttcgccgg ccattttttt
    11221 atgtctcctg tttgtgctgc gattcgaaca atcggcaaat taggttattg tttgttgtcc
    11281 aatcgggaca gccaatttgc gtatcgcttc attatactca gtcactcagc ctctgcaatc
    11341 ctttgcgaca ttggaacaga aaatcatttt gcatattttt tgacgatttc ctgcatttta
    11401 tcgaatttat tttccatgtc ctgtaccatt ttgaactgcg tgcggcagcg gtcgaggttg
    11461 tgattttcgc gatgaacttt gaaataaacg tcgccggaga gatggtcagc cagaaatcgc
    11521 aagcctattt cgaaggttat gagttttgcg gaaaacgcga gaagctccag ttctcgcggc
    11581 gtcaggaaat cctttgccgc cgacagatat ccgtgcgtca gcctgtcgaa aagttcgagc
    11641 gacatggaga ctttgtccag gtcgcgttcg tcctcgacgg cggtggcggc gcctatccgt
    11701 accgagtcgc cgaagtcgta catcgctgcg cctggcatga cggtgtcaag gtctatgacg
    11761 cagatgccct tgtcagtcgc gtcgtctatc atgacgttgt tgaattttgt gt
//
LOCUS       DAONFI010000017        11718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6145_length_11718_cov_3.996485, whole genome shotgun sequence.
ACCESSION   DAONFI010000017 DAONFI010000000
VERSION     DAONFI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6145_length_11718_cov_3.996485"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLK08_01115"
     CDS             complement(<1..653)
                     /locus_tag="PLK08_01115"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPS52128.1"
                     /translation="MSLIGMGNTNGRTLNHEEVRQVISQAAASISPAGKKVLVIIPDH
                     TRTCPLPQVVRELYEFIGKKASKFDLLIALGTHTAMTDEQIDRLLGVEPGRRGEVFPD
                     VNVFNHEWKNPESLRTIGGFTRDEVLDYLPADCEIFARDIDVSVNVRLFDYDKVLICG
                     PVFPHEVVGFSGGAKYFFPGVCGEELLNFFHWLGALITIPKMIGIKDTPVRRMLHAA"
     gene            856..3165
                     /locus_tag="PLK08_01120"
     CDS             856..3165
                     /locus_tag="PLK08_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HPS52129.1"
                     /translation="METIAHRTEDGREQRLGEHLTSVSESAGRFAADFNVKELGEITG
                     LLHDVGKYTAEFQKYIRRLQNRSLQTFHAAAGAWQALALSAEHKNSPAFQLAAMAIQG
                     HHSGLHKPTLFLYDKGWKSQAQNIFNRAVKGGWMCGKYDNAPSLGAWLDLTAKLSKAG
                     CYEMQCRLECLTRLMFSALIDADRLDSEKFAEPEKFAMRGDFSNHEKILSELLAKLEA
                     HMAKFRADTPINILRAEMLQDCESKASQPSGVFSLAMPTGGGKTLAAMAFAFRHALAN
                     HLHRIIVAIPYTSIIDQNAAIYADIFGEQNVLQHHCNLDPDRYNGDEDESQKLLAEEL
                     ACENWDRPIIVTTNVQLLESLFAASPSRCRKIHNVARSVIIFDEAQTLPPAMLEPTLD
                     MLKIFVKFAGTSLICCTATQPAWKTIRDDVVGDISLKGIDDISEIISQPEIWHKKLSR
                     VKYHWPSDMTQTTTWEELASKIVEHPRCLVIVPFREHVRKLISLLPDGTIGLSALMTP
                     VHRKKVLDDIKRNMADKNAVCRVIATSLVEAGVDLDFPVVFRAIGPLDSIAQAAGRCN
                     REGLLENGGDVHIFIPPENHQKSTVIGRGTDTTVTLVNAFRNSGAEPDMSDPTIFQKY
                     FEELFKISNVDKLAVQASRRELDFPETQKRYHIIDDGWSASIIIPQDAKAAAALAVIT
                     QHNSGQKFSSAAELRKARSTLQQYTVAVSKKIAGAWISAGRIASLYQAADFIDGPYYL
                     NDVFLENGYDDLGLKLGEIPKMSTGKTII"
     gene            3274..3951
                     /gene="cas5c"
                     /locus_tag="PLK08_01125"
     CDS             3274..3951
                     /gene="cas5c"
                     /locus_tag="PLK08_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015487358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HPS52130.1"
                     /translation="MGSPPRMSIKVGGDYACFSRPEFKAERASYPWITPSAARSIFEA
                     VLWKPEIRWEIRRIKLLRPVKFTNFRRNEINTKIPPSKAAHGKAELYTDSDRAQRNTL
                     ALKDVEYILEADLFLTDKGLAGGENIVKYVEMFQRRLEKGQHFHQPYLGCREFAARVS
                     PPRGDEDAIDENMDAGLMFYDFSVFPGVDKNGKTKGKAVPLFFRANMTNGIIEVQPKS
                     EIERGVS"
     gene            3948..5873
                     /gene="cas8c"
                     /locus_tag="PLK08_01130"
     CDS             3948..5873
                     /gene="cas8c"
                     /locus_tag="PLK08_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HPS52131.1"
                     /translation="MILQELVKLCDSEELIPMPDFQWRQTHWHINIDANGKLLGPPVC
                     VKEEIPVPNTNNRTITQGLDTILPWTIQPRTNEPESEFLCDNITYTFGFYSKEAKKLD
                     KKMSLYQRYLIDALSATNDPGISAQLKCINMIRTKPQMLFNYWGDFSQKGKKAKDAEG
                     NSIFLPKEWEDTDVFVFKFENKLVVHRQPVIDYWQRLRQNRREELPKGQCLISGKIAA
                     LALKHMQVKLGTASADPKGCSVVSFNNNAFTSYGLKKNANAQISQENMEKYAVALQML
                     LSPVGGKSKNVKCAVHNYLLSKEIRAVFWVDYPKKDSTITEILQGCFHGNISVIKELL
                     DSPHTGKQIEINNAVNFLCFTMAAAKSRVIILSDLKTTLPKLQQALLAYYNDIELHTN
                     EHEPSLCLKNILESAKPEGKNCEGSRNQINAFFQAIVMFTPYPLGILNQTITKSLVVV
                     RDKRGKARYIDTNLFRLIKAILNRNFNYPIQSTLEKTMNAMRKQKEDSIKNKKNFQSY
                     QLGRIFAVIEKAQKEAINSNEELNTRFLKSATMTPARTFLHLLEMFPNYIAKLNKSGR
                     NEKEEKLKKYWAQECRNTMAEIFADINSDFNPEDIENLLPKRLNLEEQGILLMGYYQQ
                     RASFFTKKENGQETDQE"
     gene            5911..6858
                     /gene="cas7c"
                     /locus_tag="PLK08_01135"
     CDS             5911..6858
                     /gene="cas7c"
                     /locus_tag="PLK08_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HPS52132.1"
                     /translation="MSNLTSDLYNRYDMVLIFDVTDGNPNGDPDADNQPRIDIETNQG
                     LVTDVCQKRKVRDYVANRLGMEIFFKHQGILNNFIDDAHRQADAKSKKSNPEKSKCTR
                     EWLCKNRYDIRTFGAVLDTGEKDKKAGQVRGAVQCTFARSIDPILYMNFTITRKSVTT
                     NKEAEAQIKDNGYIIGTMGRKSLVLYGLYKSNWFVNPALAHQTGFSKADFKILCDALL
                     NMWEDDHSASRGMMTTCALYVFEHDATESGKPALGKAPSHLLLESVKIEKIKAKQESG
                     EPARQFSDYAVTLDKNGWTEKVNVYNLVDPADYARLFGK"
     gene            7072..7761
                     /gene="cas4"
                     /locus_tag="PLK08_01140"
     CDS             7072..7761
                     /gene="cas4"
                     /locus_tag="PLK08_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPS52133.1"
                     /translation="MYDDDSLIPIAALQHYCYCPRQCALIHLEQAWEENQHTAAGQVL
                     HKKTHEPATAMENGVLVARGVRLCSMTLGLTGQADTVEFHPAQPSDIDAVQLANRSGR
                     WRVQPVEYKKGGPKKNSADRVQLCAQALCLEEMLNTHIEVGILFYGRIRRRVEVMLDN
                     QLREKTIGVISQTRDLIMSSLPQNLPAPCNDARCRLCSLAELCQPNLRKSAKKYFDDT
                     INLLLCDGGQP"
     gene            7758..8786
                     /gene="cas1c"
                     /locus_tag="PLK08_01145"
     CDS             7758..8786
                     /gene="cas1c"
                     /locus_tag="PLK08_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HPS52134.1"
                     /translation="MKIHDNTLYITTQGAYLSKDGTNIVVRVEKQERLRLPIHTIGGI
                     VCFGNVICSPFLLGLCGQEGVAVSFLSRNGRFLARLVGPQSGNLLLRRAQHNASSDSV
                     MAAELAKVFIAAKIANTRYVLQRNLRDHPSEISQPVQSAVSYLAGLINELRQPLSLER
                     IRGIEGDAARRYFSVFDRLLTSQRSDFMFTERSRRPPRDNINSLLSFIYTLLSHDVSA
                     ACQSVGLDPQMGFLHADRPGRESLALDMIEELRPALADRFVLTLINRQQVSAKGFKKS
                     PAGGVSMDDKTRKAILTAWQERKTDEFVHPFLNEKISVGLLPFIQAKLLAKCLRNDID
                     GYPPMIWK"
     gene            8799..9089
                     /gene="cas2"
                     /locus_tag="PLK08_01150"
     CDS             8799..9089
                     /gene="cas2"
                     /locus_tag="PLK08_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPS52135.1"
                     /translation="MLVLITYDVNVQTAEGKKRLRHIARLCQNHGQRVQNSVFECLVS
                     PAMWVELKNSLQKEIDAQTDSLRYYFLGKNWHRRVEHFGAKPSYDPQGTLII"
     repeat_region   9293..9781
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9294..9324
                     /rpt_unit_seq="gtcgctccatcacaggagcgcggattgaaac"
     gene            9836..10420
                     /locus_tag="PLK08_01155"
     CDS             9836..10420
                     /locus_tag="PLK08_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52136.1"
                     /translation="MLDYNCVACGEAMSSPESMIGKKELCPKCGKSIIVASTKTLNKK
                     TTPSSHEFKIKVCKAKIDNIKTHFTGQGKMTLYTDTWGNKMIHVIFAVPFNWFLALCL
                     AFVILLITRFIAQAIVDSGIVTFLIIPGWLIYYFLLRSFLSKNYEFVNQLTKETCLIA
                     PNKKMCFFKMNDDAWIATPSNAERSNIVSLYIQD"
     gene            10507..11127
                     /locus_tag="PLK08_01160"
     CDS             10507..11127
                     /locus_tag="PLK08_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52137.1"
                     /translation="MREAKQDYKDKTEILTNQKAMAENTCKSLMGTINNLPREISLAK
                     NIASDKTMPQNSRNSAKKNIKRYSKELAQAKIDFKKNQETLLRSSAEFSEIQKQVPGE
                     TYFKKIDKTNMSKERCGQILDGKFKVLEIIDDTRVIAEMAIEPYYVKGSTKLKYHTIQ
                     VQIKTESTQKLKSKQIVKTNEVYQIIATNSNEGKPQLILEPVKISN"
     gene            complement(11305..>11718)
                     /locus_tag="PLK08_01165"
     CDS             complement(11305..>11718)
                     /locus_tag="PLK08_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HPS52138.1"
                     /translation="RQVFTSMWTSAEDFKAQHIALTERADIMLVVPATANIISKFAAG
                     QADDLVSTLALSAFGACEILIAPAMNTRMWKAHPTQENIEKLKKWHMNIVGPDCGHLA
                     CGTTGEGRLADLDDIYAAAEQLLLKNPPKKANETD"
BASE COUNT         3472 a         2492 c         2701 g         3053 t
ORIGIN      
        1 catgcggcgt ggagcatgcg acgaaccggc gtgtctttta tgcctatcat tttcggtatg
       61 gtaataagcg ctccgagcca gtgaaaaaag ttcaacaatt cctcgccgca tacgccgggg
      121 aagaaatatt ttgcaccgcc ggaaaatccc acgacttcat gcggaaagac cggcccgcaa
      181 ataagcacct tgtcgtaatc gaaaagacgt acatttacgc ttacgtcgat atcgcgtgca
      241 aaaatctcac agtcggccgg cagataatcc agaacttcgt cgcgagtgaa accgcctatc
      301 gtgcgtaaag attccggatt tttccattcg tgattaaaaa cattgacgtc cgggaaaact
      361 tctccgcgtc tgccgggttc tacgcccaga agcctgtcta tctgttcgtc ggtcattgcc
      421 gtgtgcgtgc cgagtgcgat taaaaggtcg aatttcgacg cctttttgcc gatgaattcg
      481 taaagctcgc gaacgacctg cggcagcggg catgtacggg tatggtcggg tattatcaca
      541 agcacttttt tgccggcagg tgagatgctc gcggcagcct gcgaaattac ttgccttacc
      601 tcttcatggt tcagcgttct accgttcgta ttgcccatgc cgattaagct catatatatt
      661 gctccgttgc tgatgatgta aatttcaaac tgtgaacgtt catgtatggg aaatgtattc
      721 aaatacattt tatccgcatg tatcatgttt accaagccaa agggcggttt tacaacagtt
      781 acggacgata cggcaatgcg cgtgatttcg ttttgacgtt tattacggcg ttgtcgtttg
      841 atatactttc attttatgga aaccatagct catagaacgg aagacgggcg ggaacagcgg
      901 cttggggaac acctgacatc ggtttcggaa tcagccggca gatttgcagc tgatttcaac
      961 gtgaaagaat tgggcgaaat aacaggcctg ctgcatgacg tcggcaaata taccgcggaa
     1021 tttcaaaaat atatcaggag actgcaaaac aggtcgttgc agacgtttca cgcggcagcc
     1081 ggagcatggc aggcgttggc attatcggct gagcataaaa acagtccggc atttcagttg
     1141 gcagcgatgg cgatacaggg acatcattcc ggcttgcata aacctacgct ttttttgtat
     1201 gacaaaggct ggaagtcaca ggctcaaaac atttttaatc gtgccgtcaa aggcggctgg
     1261 atgtgtggta agtacgataa tgctccaagc ctcggagcgt ggctggattt gacggcgaag
     1321 ttgtcgaaag ctggctgcta tgaaatgcag tgtcgtcttg aatgccttac gcggctgatg
     1381 ttttccgcac tgatagacgc cgaccggctc gattccgaga aatttgccga gccggaaaag
     1441 ttcgccatgc gcggcgactt cagcaatcat gaaaagattt tgagcgaact gcttgcaaag
     1501 ctggaagccc atatggcgaa atttcgtgcc gatacgccaa taaatatcct gagagccgaa
     1561 atgctgcagg attgcgagag taaggcgtcg cagccgtcgg gtgtgttttc gctggctatg
     1621 cccacgggcg gcggcaagac gctggcagct atggcgtttg catttcgaca cgcactggcc
     1681 aaccatctgc accggataat cgtggctatt ccgtacacaa gcatcatcga ccagaacgcg
     1741 gcaatctatg ccgatatttt cggcgaacag aatgtcttgc aacatcattg caatcttgac
     1801 ccggaccgct acaacggcga tgaagatgag tcgcagaaac tgctcgctga agaattagcc
     1861 tgcgaaaact gggacaggcc gattatcgtc accaccaacg ttcagttatt ggagtcgctt
     1921 tttgcggcgt cgccgtccag gtgtcgcaaa attcataacg tcgcccgaag cgtaatcata
     1981 ttcgacgaag cgcagacctt gccgccggcc atgttggagc cgacgctgga tatgctgaaa
     2041 atttttgtca aatttgccgg cacgtcgctc atatgctgta cagctaccca gccggcgtgg
     2101 aagaccatcc gcgacgatgt tgtcggtgac atatcattga aaggtatcga cgatatttcg
     2161 gaaatcatca gtcagcctga gatttggcat aaaaaacttt cccgcgtcaa atatcattgg
     2221 ccatcggaca tgactcaaac aaccacgtgg gaggagctgg cctcaaaaat tgtcgaacac
     2281 ccgcgatgtc tcgtaattgt gccgtttcgc gagcatgtcc gcaaattgat aagcctgctg
     2341 cccgacggca caatcggtct gtccgcattg atgacgcccg ttcatcgcaa aaaagtgctc
     2401 gacgatataa aacggaacat ggcagataaa aacgccgtgt gccgggtgat tgcaacgtca
     2461 ctcgttgaag ccggcgtgga tttggacttt cctgtggtat ttcgcgcgat tgggccgttg
     2521 gattccatcg cccaagccgc cggccgatgc aatcgcgaag gtttgcttga aaatggcggc
     2581 gacgtccata ttttcattcc gccggaaaat catcaaaagt ctacggttat cggcagaggt
     2641 acggatacaa ccgtgacgct tgtaaacgcg ttcagaaaca gcggtgccga accggatatg
     2701 tccgacccga cgattttcca aaaatatttc gaggaacttt ttaaaatcag caatgtcgat
     2761 aagctcgccg tgcaagcttc aagaagagaa ttggattttc cggaaacgca gaaaagatat
     2821 catataatag atgacggctg gtcagcctct attataattc cgcaggacgc gaaagccgcc
     2881 gctgccctgg ccgtcatcac gcaacacaat tccggccaaa aattttcttc tgccgccgaa
     2941 ttgcgcaaag cccgttcaac gcttcagcaa tataccgttg ccgtatccaa aaaaattgcg
     3001 ggagcgtgga tatctgccgg caggattgcg tcgctgtatc aagctgcaga ttttatcgac
     3061 ggcccatatt atttgaatga cgtttttttg gaaaacggct atgacgacct gggcctgaaa
     3121 cttggggaaa tcccaaaaat gtcaacgggg aaaacaatta tctgaaattt tccttgaaaa
     3181 agttttggta tttgttaggt tgagaatatt gccactactg tggcggcaaa agggtagttc
     3241 agttcttttt attcgaaagg aaggtttttt actatgggtt ctccgccaag aatgagtatc
     3301 aaagtcggcg gcgattatgc gtgtttttcc aggcctgagt tcaaggctga acgtgcaagt
     3361 tatccctgga taactccatc ggcggcacgt tcgatttttg aagccgtcct gtggaagccg
     3421 gaaatccgtt gggaaatccg gcgaatcaaa ctgctgcggc cagtgaagtt caccaacttt
     3481 cgccgcaacg agataaacac gaaaattccg ccttcaaaag cagcccacgg caaggctgag
     3541 ctgtataccg actccgaccg cgcacagcgg aacacgttgg cgcttaagga tgtagagtat
     3601 attttagagg ctgacctttt ccttacggac aagggccttg ccggcggcga gaacatcgtc
     3661 aaatatgtcg agatgtttca acgacggctt gaaaaaggtc agcacttcca tcagccgtat
     3721 ctcggctgcc gcgagttcgc cgcccgcgtc tctccgcccc gcggcgacga ggacgccatt
     3781 gacgaaaata tggatgccgg cctgatgttt tacgatttca gcgtttttcc cggcgtggac
     3841 aagaatggca aaaccaaagg caaagccgtc ccgctgtttt tccgcgcaaa catgacaaac
     3901 ggcataatcg aagtccagcc gaaaagtgaa atcgaaaggg gtgtatcatg attcttcagg
     3961 aactcgtgaa actctgcgac agcgaagagt tgattccaat gccggacttc cagtggagac
     4021 agacacattg gcacatcaat attgatgcta atggaaaact tttagggcca ccagtttgtg
     4081 taaaagagga aataccagtt cctaatacca acaacagaac tataacacaa ggccttgata
     4141 cgattctccc atggacaata caaccaagaa ccaatgaacc ggaaagtgaa tttttgtgcg
     4201 ataatattac ttatactttt ggtttttatt ctaaagaggc aaagaaactg gataaaaaga
     4261 tgtctcttta tcaacgatat cttattgatg ccttgtcagc aacaaatgat ccaggtatca
     4321 gcgcacaatt aaagtgcatt aatatgatta gaaccaagcc gcaaatgtta tttaattatt
     4381 ggggcgattt ttcacaaaaa ggtaaaaaag ctaaagatgc ggaaggcaac agtatttttc
     4441 ttcctaaaga gtgggaagat acagatgtat tcgtttttaa atttgaaaat aaattagttg
     4501 tacatcgtca accggtcata gattattggc aaaggcttag gcaaaataga agggaagaat
     4561 tacccaaagg acagtgccta atatctggca aaattgcagc attagcatta aagcatatgc
     4621 aagttaagtt gggaacagct tcagccgacc ccaaaggttg ttctgttgtt tctttcaata
     4681 ataatgcttt cacctcatat ggtttaaaaa agaatgcaaa tgctcaaatt agtcaagaaa
     4741 acatggaaaa gtatgcagtt gcattgcaaa tgctgctttc acctgttggt ggaaaaagta
     4801 aaaatgttaa atgcgctgta cataattatt tattgtcaaa ggaaattagg gctgtatttt
     4861 gggtagatta ccctaaaaag gatagtacta tcacggagat tttacaaggt tgttttcatg
     4921 gaaacatatc tgtgattaaa gaattacttg attctccaca tactggcaaa cagatagaaa
     4981 ttaacaacgc cgttaatttt ttatgtttta caatggctgc ggcaaaatca cgtgtcataa
     5041 ttttgagcga cctgaaaaca accctaccta agttacaaca agcccttttg gcatattata
     5101 acgacattga actccataca aatgaacacg agcctagtct ttgtttaaaa aatattctcg
     5161 aatctgcaaa accggaagga aaaaactgcg aagggtctcg taatcaaata aacgcgttct
     5221 ttcaagcaat tgtaatgttt accccatatc cattgggaat attgaaccaa acaatcacaa
     5281 aaagtttagt tgtagttcgt gataaaagag ggaaagcacg gtatatagat acgaatttat
     5341 ttcgtttgat taaggctata ctgaatcgaa actttaacta tcctatacaa tcaacattgg
     5401 agaaaaccat gaatgccatg cgaaaacaaa aagaagattc gataaaaaat aaaaaaaatt
     5461 tccaaagtta tcaattaggc agaatttttg ccgtaattga aaaagcacaa aaagaagcaa
     5521 ttaattccaa tgaagaatta aataccagat ttctaaaatc tgctaccatg actcccgcaa
     5581 gaacatttct tcacttactc gaaatgtttc ctaattatat agcaaaactt aataaaagtg
     5641 gtcgcaatga aaaagaagaa aaactgaaaa aatattgggc ccaagaatgt cgtaatacga
     5701 tggcagaaat ttttgctgat ataaattctg acttcaatcc agaagatatc gagaacttac
     5761 tgccaaaaag attgaatctg gaagaacaag gtatattact aatgggatat tatcagcaac
     5821 gtgcatcatt ttttactaaa aaagagaatg gtcaggaaac tgaccaagaa taatctaagc
     5881 atataacatt taaatgaaag gaaactaaca atgagtaatc ttactagcga tctttataac
     5941 aggtatgaca tggtattgat ttttgatgtt actgacggca atcctaatgg cgatcctgat
     6001 gccgataatc agccccgaat tgatattgaa actaaccaag gtttggttac tgatgtatgt
     6061 caaaaacgta aagttcgtga ttacgttgca aataggcttg gaatggaaat tttctttaag
     6121 catcaaggta ttctcaacaa ttttatagat gatgcgcaca ggcaagctga tgccaaatcg
     6181 aagaaaagta atccagaaaa gagtaaatgc acacgcgagt ggttatgtaa aaatagatat
     6241 gatataagga cttttggcgc agttcttgat acaggagaga aagacaaaaa agcaggtcag
     6301 gttagaggtg ctgttcagtg cacgtttgct cgctcgatag atccaatatt atatatgaat
     6361 tttaccatta cccgtaagag tgttacgaca aacaaggagg cagaagcaca aattaaagac
     6421 aacggctata tcatcggtac gatggggcga aaaagtcttg tgttatacgg cttgtataag
     6481 tccaactggt ttgttaatcc ggctttggcg catcagacgg gtttcagcaa ggctgatttc
     6541 aaaatactct gcgatgcact gcttaatatg tgggaagacg atcactctgc cagcagggga
     6601 atgatgacaa cttgtgcatt gtacgtattc gaacacgacg cgaccgaatc cggaaagccg
     6661 gctctcggca aagccccgtc gcacctgctg ctcgaaagcg taaaaatcga aaaaataaaa
     6721 gcaaaacagg aatccggtga gccggcccgt caattcagcg attacgctgt aacgcttgac
     6781 aaaaacggct ggactgaaaa ggtcaacgtt tacaatctgg tcgaccctgc agactatgca
     6841 aggctgtttg ggaaataagt ctggctgata cggacattga ttcggcgtcg tgtcggcgaa
     6901 atgcggcatg acgccgaatc aggttcgata atctggcggt ggcagaaaag ctctatggtt
     6961 taaatccgta gcatcaattt gccgaacttt cgcctgtctg ccttatgccg gattatttcc
     7021 tctgcggttt tttaaaaaaa agctgtagaa atacgaaggc catattaaac aatgtatgac
     7081 gatgacagtc tcatcccgat agcggcgtta cagcattatt gttattgtcc gcgacagtgt
     7141 gcgcttattc atttggaaca ggcgtgggaa gaaaatcaac acaccgccgc cggccaggtt
     7201 ttgcacaaaa aaacgcatga acctgcgacg gctatggaaa acggcgtgct cgtcgcccgc
     7261 ggcgtgcggc tttgcagcat gacgctaggc cttaccggcc aggccgatac tgtagaattt
     7321 catcctgcgc agccgtcgga tattgatgcc gttcaacttg cgaatcgctc cggcagatgg
     7381 cgggttcagc cggtagagta caaaaagggc ggcccgaaga aaaactcggc tgaccgcgtt
     7441 caactttgtg cccaagccct gtgccttgaa gagatgttga atacgcatat tgaggttgga
     7501 atactttttt atggcagaat tcgccggcgt gttgaagtga tgttggataa tcagttgcgc
     7561 gagaaaacta tcggtgtgat ttcgcagact cgcgacctta taatgagttc tctgccgcag
     7621 aatctgcccg cgccatgcaa tgacgcgcga tgtcgtctat gctctcttgc tgaactatgt
     7681 cagccaaacc tgaggaaatc cgcaaaaaaa tatttcgacg acacgattaa cctgcttctt
     7741 tgtgatgggg gacagccatg aagatacacg acaatacgct ttacattacg acccagggtg
     7801 catacctgtc aaaagacggc acgaatattg tcgttcgggt tgagaagcag gagcgtctgc
     7861 ggttgccgat tcatacgatt ggcggtattg tctgttttgg taatgttatt tgtagtccgt
     7921 ttttgctggg cctttgtggt caggagggtg ttgccgtttc tttcctgtcg agaaacggac
     7981 gttttcttgc tcgtcttgtc gggccgcaat ccggcaatct tctattacgg cgggcacaac
     8041 acaatgcgtc gtccgactct gttatggcgg cggagttggc gaaagttttt atagccgcga
     8101 aaattgcgaa tacgcgatac gttcttcagc ggaatcttcg cgaccatcct tctgaaattt
     8161 cgcaaccggt acaatcggcg gttagctatc tagccggcct tataaacgaa cttcgccagc
     8221 cgctttcgct tgagcgtatt cggggtattg aaggcgatgc cgccagacgt tatttttctg
     8281 tttttgaccg gctgttgacg agtcagcggt cagatttcat gtttacggag cgttcccgtc
     8341 gcccgccgcg tgataatatc aattctctgc tgtcgtttat ttatacgttg ttgtcgcatg
     8401 acgtttctgc agcctgtcag tccgtcggcc ttgaccctca gatgggattt ctgcatgctg
     8461 accggcctgg cagggaatca cttgcacttg atatgataga ggaacttcgg ccggcgttag
     8521 ccgacaggtt tgtcctgacg ctgataaatc gtcaacaggt atctgccaaa ggtttcaaaa
     8581 aatcaccagc cggaggcgtg tcgatggatg acaaaacacg caaagcgata cttacagcat
     8641 ggcaggaacg caaaaccgat gaatttgtgc atccgttttt gaatgagaaa ataagcgtag
     8701 gtcttctgcc gttcatacaa gccaagttgc ttgcaaaatg tttgcgaaac gatattgacg
     8761 ggtatccccc gatgatttgg aaataggagc tttttgggat gctggttttg attacgtacg
     8821 atgtaaacgt tcagactgct gaaggcaaga agcggctgcg gcatatagct cggctttgtc
     8881 agaatcatgg ccaacgtgtt cagaattcgg tgtttgaatg tcttgtatcg cccgccatgt
     8941 gggtggaatt gaagaattca ttacaaaagg aaatagatgc acagacagat tcattgcgat
     9001 actattttct tggcaaaaac tggcatcgtc gtgtggagca ttttggtgca aaaccatcat
     9061 atgatccgca gggcacgctg ataatttgat tattgaactc gcccgctatg ggctgccgcg
     9121 aaccttatgt gacggcaaaa tgcccggcag attcgcgaca acggcaagta ttgcattgac
     9181 aagtaaatat gacttttata taagaaaatc gctccctgtg aatggagcgg cgacatactt
     9241 tcgcggaaat atttatgtaa catttgatgc ggcagcagtt aaggatagct gccgtcgctc
     9301 catcacagga gcgcggattg aaactcaata ggctgcgtca attacaaatc catttgcaat
     9361 cgtcgctcca tcacaggagc gcggattgaa acctatagca aaatgcaata gtatcaagtg
     9421 tgaagattgt cgctccatca caggagcgcg gattgaaact agagaaacaa aaataaaaaa
     9481 tatcatatag cgtcgctcca tcacaggagc gcggattgaa acgtcttgaa cgattaattg
     9541 acaattcggc aggtatgtcg ctccatcaca ggagcgcgga ttgaaactcg ccggcggcgg
     9601 cctgcacctg ctcggcagat agtcgctcca tcacaggagc gcggattgaa acgactaccg
     9661 tgtagacggg cagaaacgcg gattcggtcg ctccatcaca ggagcgcgga ttgaaaccac
     9721 ttccagttag agcggttgtc caaaaattag gtcgctccat cacaggagcg cggattgaaa
     9781 cgggttgact cacgccgttt ttcgccctat aatttcatat gaaaggtaca gaaatatgct
     9841 tgattataac tgtgtagctt gtggcgaagc catgtcaagt ccagaatcaa tgattgggaa
     9901 aaaagaattg tgtcccaaat gtggaaaatc tattattgtc gcttctacta aaactctaaa
     9961 taaaaaaaca actccatcaa gccatgagtt taaaattaag gtttgtaaag caaaaattga
    10021 taatattaaa acccatttta ctggtcaagg taaaatgacc ttatataccg atacatgggg
    10081 caacaaaatg atacatgtaa tttttgcagt accctttaat tggtttttag cattatgttt
    10141 agcatttgtc attttgctta ttactcgttt tattgcacaa gctattgttg attctggaat
    10201 tgtaactttt cttataattc caggttggct aatttattat tttttacttc gtagtttttt
    10261 gagtaaaaat tatgaatttg ttaatcaact tacaaaagaa acatgtttaa ttgcaccaaa
    10321 taaaaaaatg tgttttttta agatgaatga cgacgcatgg atagcaacgc catcaaatgc
    10381 ggaacgaagt aatattgttt cattatatat tcaggactaa ccatgaaaac tacactactc
    10441 ataatcgcgt tgctctgctc ggtagttttt tgccaaacgg tagaccctga cttgcaggaa
    10501 aaaataatgc gcgaagcaaa gcaggactat aaagacaaaa ccgaaatttt aaccaatcaa
    10561 aaagctatgg ctgaaaatac gtgtaaaagt cttatgggaa ctatcaataa tcttccaaga
    10621 gaaatttctc ttgctaaaaa tattgcgtcc gataagacaa tgcctcagaa ctcaagaaat
    10681 tcagctaaaa aaaatattaa aagatattca aaagaattag ctcaagctaa aattgacttt
    10741 aagaaaaatc aggaaacatt gctccgttca tccgctgaat tttctgaaat acaaaaacaa
    10801 gtccctggcg aaacatattt caagaaaatt gataaaacaa atatgtcaaa agaacgttgc
    10861 ggtcagatac ttgatggtaa atttaaggtt cttgaaataa tagacgatac tcgcgttatt
    10921 gcagaaatgg caatcgaacc atattatgtt aaaggaagca caaaactaaa atatcataca
    10981 atacaggttc aaatcaaaac agaatcgact caaaaattaa aatcaaagca aatagttaaa
    11041 accaatgaag tttatcaaat catagctact aactcaaacg aaggtaagcc tcaactgatt
    11101 ttagagccag ttaaaataag taattaaaat tttattaaac taacgatggt aaagcactaa
    11161 atgatgttga attttttaaa cttttttcac ctactgtagc agaagtaggt atctgcataa
    11221 aacaattcgc tccatcacag gaacatggat tataaatctt ggagtaaata gaacggctta
    11281 catgcaaaac gaatcagata ccctttaatc cgtttcgttc gcttttttgg gtggattttt
    11341 caaaagtaac tgttcggcgg cggcataaat atcgtccagg tcagccagac ggccttcgcc
    11401 tgttgtgccg catgcgagat ggccacaatc agggccgacg atattcatat gccacttttt
    11461 gagtttttcg atattttcct gtgtaggatg tgctttccac atgcgtgtat tcatcgccgg
    11521 cgcgatgagt atttcacatg ccccgaaagc tgaaagcgcc agcgtggaaa caaggtcgtc
    11581 agcctggccg gctgcgaatt ttgagatgat atttgccgtc gccggcacta caagcatgat
    11641 atctgcacgc tcggtcagag cgatatgctg tgccttaaaa tcttccgccg aagtccacat
    11701 gctcgtgaaa acctgcct
//
LOCUS       DAONFI010000018        11676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6183_length_11676_cov_4.213579, whole genome shotgun sequence.
ACCESSION   DAONFI010000018 DAONFI010000000
VERSION     DAONFI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_11676_cov_4.213579"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLK08_01170"
     CDS             complement(<1..936)
                     /locus_tag="PLK08_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPS52139.1"
                     /translation="MLWFKEESADISHEKMLLLAQHFAEEALNKICRNPRRVLLLPPD
                     ITRAHCGAGWITRELYRIFSSYADVHVIPTLGQHVPHTPQQNEWMFGDIPQTHIHVHD
                     WRGGSKIIGEVPADFVREVSHGKADWPIPVSLNKMLLEEKWDLILNVGHVVPHEVLGF
                     ANHNKNYFIGLAGKDMICASHMMAACCGIENNLGCLVTPLRACFNRAQDDYLKHLPDA
                     FFQMVMAYNPDGKLVHTGVYAGNGTETYLAAAKASCKQNITIVPPLKKIVAVMQGNEF
                     YSTWVANKAIYRTRMAIADGGELLIIAPGLTRFGEQ"
     gene            complement(975..1823)
                     /locus_tag="PLK08_01175"
     CDS             complement(975..1823)
                     /locus_tag="PLK08_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS52140.1"
                     /translation="MITGASAGIGAAASIALAKKGFRLCMMARNQEKLCQAADAVHNS
                     TGKKESAIIYTGDVTDENDRHGAIKKIIAEFGRLDILINNAGDALAGAIEDVSLNEAR
                     QQIELNALAPMGMIQLVGPIFRKQGSGRIINVSSISGLMSLPGLGIYSASKYALEAIN
                     DAARREYHPWGIKVSAIEPGGISTQIWNSTRDDLLHRIEKSGNSPFRNFYHEQLQQLD
                     TLLNGGTDVSEVVKVIVHAATAKKPKLRYCFQKQCRDRRMVSHLPTFIEDWIVRKYVK
                     VNLPSE"
     gene            complement(1870..2847)
                     /gene="rfaD"
                     /locus_tag="PLK08_01180"
     CDS             complement(1870..2847)
                     /gene="rfaD"
                     /locus_tag="PLK08_01180"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HPS52141.1"
                     /translation="MMKRFIVTGGAGLIGSNVVKALNNRGWREIIVVDHLNHPAKREN
                     LAQLKYTEYMDKADFRKQMKERRIPAVDCVFHLGACSSTLQTDEAYLHDNNFQYTQEL
                     CEWSIKNGTRFVYASSAATYGDGSCGYCDDDAVTKKLIPLNPYGRSKQEFDLWAMSGG
                     VLDKIAGLKYFNVYGPGEDHKGEMQSLIRKSYDRINQTGEITLFKSHRPDYTDGMQQR
                     DFVYVEDAVNVTLFFYDHPEISGLFNCGTGCARTWIDLANALFAAMNRPSKIRFVDMP
                     QSIRDKYQYHTQADMTKLRRAGYTAPFAGIEEGVRKYVQEYLQIQKKVV"
     gene            complement(2973..3725)
                     /gene="recO"
                     /locus_tag="PLK08_01185"
     CDS             complement(2973..3725)
                     /gene="recO"
                     /locus_tag="PLK08_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPS52142.1"
                     /translation="MRYHDRAICLRSLDYSETSQIIHFLTRENGVVHLLAKGTKRKKS
                     KTGGAIDLLCEGEVIYTKKDSDTLGMLMEFSENVSRSELRKDASRLNAALYAIELVYA
                     MLPEGLAQSEIFTLLSNTLRRLAEKDSPQNAVLAWFQWRLLRHVGLLGGLDSCVSCGM
                     SVEEMAHDDEVFFTSRYGGLLCSRCLAPGGEKKQADKIVLGALAALTAAEAGKKVNLP
                     PNQAIAIMKLLDYHISQQLGKQLKMARYVIKT"
     gene            3870..5414
                     /locus_tag="PLK08_01190"
     CDS             3870..5414
                     /locus_tag="PLK08_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52143.1"
                     /translation="MEIFAKIRKFFDIVLHVGPMAESIWVNVSTAAVRLGIGVLRIFI
                     FARLMAEVEMGVWGTATMGMTIVAPLVNLGMNQGFERYVSVYESAGRLRWFFRRGFLG
                     AITVTIVLAILAACFYPAWRKMLFPVSLGYTSWRSGLLTWLVIVNVAALGVYFLLTGF
                     LAGLRMFRLTAFLDLLFSILTTFAAAGVLYFYRSATAVLLVHLLSVLACFSIGTAMLF
                     IGLHRVKSSDFLTVLDGDDSGFKPADNFLKILLQLGRFGFVGLMGTTVWLIVTFVSYY
                     LTYLTDGRAVAGRYTVMLRLAQPIYTLAIAIWAVIYTHTAHTWQHGDKKFAMERMDVM
                     FRRVMAMVLVGGWAIILSEPAWSRILGPKYCGAGGYLPGLLMMFAAMSSVLSLFTWGR
                     ILGRQWSIVIAGAAAVVLNVAAYYAFERFFITKMFSSLSAGVGVFAGSVMMTVFYFTH
                     WKIKISIRSAVMLLLPAIFLLSNGIANSLCAAAITLVMCYFAFTPAERKRLFDLKNYS
                     NQSRLF"
     gene            complement(5788..5982)
                     /locus_tag="PLK08_01195"
                     /pseudo
     CDS             complement(5788..5982)
                     /locus_tag="PLK08_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346944.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            complement(6257..6328)
                     /locus_tag="PLK08_01200"
     tRNA            complement(6257..6328)
                     /locus_tag="PLK08_01200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6294..6296),aa:Thr,seq:cgt)
     gene            6487..6714
                     /locus_tag="PLK08_01205"
     CDS             6487..6714
                     /locus_tag="PLK08_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPS52144.1"
                     /translation="MREKVQEVIDQIKPMLQADGGDIELVDVNEKTGVVSVRLQGACR
                     GCPGARMTLKMGVERKLQSEIPGVTEVLAVD"
     gene            6756..9392
                     /gene="mutS"
                     /locus_tag="PLK08_01210"
     CDS             6756..9392
                     /gene="mutS"
                     /locus_tag="PLK08_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPS52145.1"
                     /translation="MSLNPDVQADTPAMRQYRTFKEQYPDYVLLFRMGDFYETFYEDA
                     KIASRTLGLTLTSRSKGPNAVPLAGIPYHALDNYLGRLIQAGYRVALCEQIEDPKEAK
                     GVVKRDVVRLITPGTLTDETLLEQREGNYLAAIFSVGRKPQNCDSPSVGLAWVELSSG
                     AFWAMLAPAQTALDELVRINPAEVIYPEGSEYDQPKWRDHAKQVIGCAFSSRAPWAFD
                     KNSSVQTLHNHFHTATLAGFGFDEWDESVSAAGAIIEYLNETQKTAMGHICELRRFER
                     RDYMAIDGNTLRCLEVLRTLRTNSRQGSLLACIDRTKTGMGARLLERWLTFPLVCYSS
                     IIARQDAVEFFISRRKSLDALRELLGDCAQIDRITTNIAMARVRPREFVALGQSLALL
                     PRVAEYLDEDADKLELLTRLRPAIENLDEPLALIETAIDPDCPNVVRDGGVIRNGFNE
                     ELDRLRAIDRDGQSWLAEYQQQESQRTGIPLKVGYNKVFGYYIEITHTHRDKVPPEYH
                     RKQTLKNAERYITDELKHYEAEVLTASERAKEIEQQLYEQVRVSLLPWIEKLQRAAES
                     LATLDVLSSLAQVALERGYIRPEITQDNVLNIVGGKHPVVAELLREQFVPNDVKMGNV
                     KDRLLIITGPNMAGKSTYIRQAALLTLMAQTGCYIPAESAIIGIADRLFTRVGAADEL
                     ARGLSTFMLEMVETANILNNATQRSLVILDEVGRGTSTCDGLSLAWAICEYIALNIKC
                     RTLFATHYHELTELEDLIDGVKNLNIAVREWKGDVIFLHKIIPGGTDRSYGVHVAKLA
                     GVPKDVINRAQAILPQMQAQLADVLDMPDLADKARAAAAQMNLFADPAARIAGEIRHA
                     DLDNMTPIQAYELLRKLKDELS"
     gene            complement(9428..9850)
                     /locus_tag="PLK08_01215"
     CDS             complement(9428..9850)
                     /locus_tag="PLK08_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52146.1"
                     /translation="MLRFHCSDIDILTDRIASVTGPRSKVYGNIILQIGADKFNYGYF
                     PLDKNWEVTPKDIFERRYEYAVRLCDDFQTSGGVKPMPDKMPAWRNKNIIDAVCRNFG
                     TKIDTFVIPGYGAAEIYHLDWNKPAATDQNLMTKKFKT"
     gene            complement(9904..10989)
                     /locus_tag="PLK08_01220"
     CDS             complement(9904..10989)
                     /locus_tag="PLK08_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPS52147.1"
                     /translation="MMINSQKNFRLDIIMLALLELLLCVVYLKTVPRFYGDESWEASI
                     GYNIAREGSIRNGAIDGWGGTNVHFLQNQLIQPLFCSISFLIFGYGVIPARLPSVLMA
                     VITVCTTYAIMHKLFTRRAAILCAIMVAVNPWFFIIARRVRPEIFALAFAMLFLMMML
                     KIGRGKLAWPIIAGICSAIASLSHPNGFVLCGCIFVSVTLWRRPESFFKAVLVMAISF
                     IITVSPYLIYVAAAINNPNVSFAAQMTGGKLREAWSVTYLLQSEFGRWLRYFQWPKGL
                     PLMILYACSLMAAFIKSKPADRIIGTVPIVFALVLPFTTVNTAAVYLSLLSPFFAMLL
                     TRFIQRIWWKTRIKFLINTALKTGRYG"
     gene            complement(10979..11644)
                     /locus_tag="PLK08_01225"
     CDS             complement(10979..11644)
                     /locus_tag="PLK08_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HPS52148.1"
                     /translation="MTPARDFLKLYRLMFCRFGHQHWWPGDTPFEICVGAILTQNTSW
                     QNVEKAIANLKKNRCLSLQKIDAMPPAELAELIRPAGYFNIKTKRLQNFTSHVKKNHH
                     GRLADMFRQSEKILRNELLDISGIGPETADSIILYAAGKASFVIDAYTRRVFSRHGLC
                     DKDADYHDLKKLCEDNLPHDTELFNDYHAQLVAVAKKYCKTKPVCKGCPLENFLRKSK
                     YDD"
BASE COUNT         2769 a         2794 c         2880 g         3233 t
ORIGIN      
        1 ctgttcgccg aaccgtgtta gcccaggcgc aattatcaat aattctccgc cgtcggcgat
       61 agccatgcga gtgcgataaa tcgctttatt tgcaacccac gtactgtaaa attcgttacc
      121 ctgcatgacg gcgacaatct tttttagcgg cggaacaatc gtgatatttt gtttgcatga
      181 cgcttttgcg gcggcaagat acgtttccgt accattgcct gcgtaaacac cggtatgcac
      241 gagttttccg tcaggattat acgccatcac catttggaaa aaggcatccg gcagatgttt
      301 tagataatca tcctgagccc tgttgaagca tgcgcgcaac ggcgtaacca ggcagccgag
      361 attattttct attccacagc aggccgccat catgtggctg gcacatatca tgtccttgcc
      421 ggcaaggccg atgaaatagt tcttgttatg gtttgcaaat ccgagcactt catgcggaac
      481 aacgtggccg acgttcaata tcaaatccca tttttcttcc agcagcattt tgttgagcga
      541 gaccggaatc ggccaatctg ctttgccgtg tgaaacctcg cgcacaaaat ccgcaggcac
      601 ttcgccgatg attttcgacc cgccgcgcca atcgtgtacg tgaatatgcg tctgtggaat
      661 gtctccgaac atccactcat tctgttgagg cgtatgcgga acatgctggc ccaacgtcgg
      721 gattacatgt acgtcggcgt aagatgaaaa aattcggtat agttcccgtg ttatccatcc
      781 agcaccacaa tgtgcgcgcg taatatccgg cggaagcagc aggacgcgac gcgggttacg
      841 acagattttg ttcaatgctt cttcagcgaa atgttgtgcc agcagcagca ttttttcgtg
      901 tgatatatcg gctgattctt ctttaaacca tagcatctca gaaccttctg gtaaaaattt
      961 cctgatttcc gacactactc agacggcaga ttaactttga catattttcg tactatccag
     1021 tcttctatga atgtcggcag gtgagatacc atgcgccggt cgcgacactg tttctggaag
     1081 cagtaccgca atttgggctt ttttgccgtt gctgcatgaa caatgacctt tacgacctca
     1141 cttacgtctg tgccgccgtt aagcagcgtg tcaagctgct gcaactgctc gtgataaaaa
     1201 ttcctgaacg gactgttgcc ggatttttca atccgatgga gcaggtcgtc tcttgtagaa
     1261 ttccatattt gtgttgaaat gccgccaggc tcaatagccg atacttttat gccccaggga
     1321 tgatattcgc ggcgtgcggc atcattgatg gcttcgagag cgtatttgct ggctgaatat
     1381 atacccaggc caggcagtga catcaatcct gaaatcgaac tgacatttat tattctgccg
     1441 ctaccctgtt tgcgaaatat cgggccgacg agctgtatca tgcccatcgg cgcaagtgcg
     1501 ttgagttcta tctgctggcg ggcttcgttg aggctgacat cttcaatcgc gccggctaat
     1561 gcatcgccgg cattatttat gagtatgtcc agccggccaa attcggctat gatttttttt
     1621 attgcgccat gcctgtcatt ttcgtccgta acatcgccgg tataaattat ggcggattct
     1681 tttttgccgg tcgagttgtg gactgcgtcg gcagcctggc agagcttttc ctgattacga
     1741 gccatcatgc aaaggcgaaa tcctttttta gccagtgcta ttgacgccgc tgcgcctatt
     1801 cccgcacttg ctccggttat caatatcgtt ttgccttcaa aattattttt tttggaaaac
     1861 agcatctttt taaacaactt tcttttgtat ctggagatat tcctgtacgt attttcgcac
     1921 gccttcttca atgcctgcga acggagcagt ataacctgcc cgccgcagtt tggtcatgtc
     1981 agcctgcgtg tgatattgat atttatcacg gatggattgc ggcatatcga caaagcggat
     2041 ttttgacggc cggttcatcg ccgcaaacag cgcatttgcc aaatcaatcc acgttctcgc
     2101 acagcctgtg ccacaattaa acaggcctga aatttcagga tgatcataaa aaaacagcgt
     2161 cacgtttaca gcatcttcaa cgtaaacaaa atcccgctgc tgcataccgt cggtgtaatc
     2221 cggccgatgc gatttaaaca gtgtaatttc gccggtctga tttattctgt catacgattt
     2281 gcggataagc gattgcattt cgcccttatg gtcctcgcca gggccgtaaa cgttgaaata
     2341 tttaagtccg gcaattttgt cgagcacgcc gcctgacatt gcccataaat cgaattcctg
     2401 tttgcttctg ccgtacgggt ttaacgggat gagctttttc gtgacggcat cgtcgtcgca
     2461 atacccgcaa gaaccgtcac catatgtcgc agcgctggat gcgtaaacga atcttgtgcc
     2521 gtttttaatc gaccattcgc aaagctcctg cgtatattgg aaattgttgt catgcagata
     2581 agcttcatcg gtttgcagag tggaactgca cgcaccgaga tgaaatacgc aatcgaccgc
     2641 cggtatacga cgttctttca tctgcttgcg gaaatccgcc ttgtccatat attcggtata
     2701 cttcaactgc gcgaggtttt cccgcttggc cgggtgattc aggtggtcaa ctacgattat
     2761 ttcccgccag ccgcgattgt tcagggcttt taccacattg cttccgataa gccctgcgcc
     2821 gccggtcact ataaaccgtt tcatcataga atataaatct ttcgtgttac tgttttttaa
     2881 aaaatattac gattagggta acaattccaa ccatcaaatc aacgataaat tccgttgaac
     2941 atttcgccgc aaagattgga cggatgactt tatcatgttt tgattacgta tcttgccatt
     3001 ttgagttgtt taccgagttg ctgggatatg tggtaatcta gcaatttcat gattgcgatg
     3061 gcctggtttg gcggcaggtt gacttttttg ccggcttctg cggcggttaa tgccgcaagt
     3121 gcgccgagta cgattttatc tgcctgtttt ttttctccgc ctggtgcgag acagcggctg
     3181 cacagcaatc cgccgtagcg agaagtaaaa aatacttcgt cgtcatgagc catctcttct
     3241 acgctcatgc cgcacgatac gcacgaatcc agtccaccca gcaaacctac gtgccgcaac
     3301 aaccgccatt gaaaccatgc gagcaccgcg ttttgcggcg aatctttttc cgccagccgc
     3361 cgcagcgtat tgctcaacag tgtaaaaatt tccgattgag ccaagccttc cggcaacatg
     3421 gcataaacca gctcgattgc gtataaggct gcgttcaatc gggaggcgtc tttcctgagt
     3481 tcactgcggg aaacgttttc actgaattcc ataagcatgc cgagggtgtc ggaatctttt
     3541 ttggtataaa taacttcacc ttcgcagagc agatctattg caccgccggt tttgcttttt
     3601 ttacgtttgg ttccttttgc gagcaggtgc accacgccgt tttcgcgcgt gagaaaatgg
     3661 attatctgcg acgtttcgct gtaatccagg cttcgaagac atattgctct gtcatggtat
     3721 ctcataatgt taatatatgg ttgtaattcc acagctttcg atagccgatt agcatcggct
     3781 gccgtaagta aatttccgaa cagtcaggat accagtaacg ccaaaaaaat tcatccgaat
     3841 ttatggctga ccggttataa tccgcacaga tggagatttt tgctaaaata cgaaaatttt
     3901 tcgatattgt gttgcacgtc gggccgatgg ctgaatcgat atgggtaaac gtgtcgacgg
     3961 cggcagtacg gctcggcata ggagtattgc ggatatttat ctttgcaagg ctgatggctg
     4021 aagtggaaat gggcgtctgg ggaacggcta ccatggggat gaccatagtt gcgccattgg
     4081 ttaatctggg catgaaccag gggtttgaac gatacgtaag cgtgtatgag tccgcagggc
     4141 ggttgcgatg gtttttccgg cgtggatttt taggggcaat tactgttaca attgtgctgg
     4201 cgatattggc ggcatgtttt tatccagcct ggcggaagat gctgtttccg gtttctcttg
     4261 gttatacttc ctggcggagc ggactgctta cgtggctggt tattgtgaac gtagctgcgc
     4321 ttggcgtgta ttttttattg acgggttttc tcgccggcct tcggatgttc aggctgacgg
     4381 cgtttttgga tttgctcttt tcgatattga ctacttttgc ggctgccggc gtactttatt
     4441 tttatcggtc tgccacggct gtgctgctgg ttcatctgct atcggtgtta gcctgttttt
     4501 caataggaac agccatgctt ttcatcggct tgcatcgcgt taaatcatct gattttttaa
     4561 ctgtactcga tggcgacgac agcgggttca agcctgccga taattttctc aaaattcttt
     4621 tgcagttggg ccgattcggg tttgtcgggc tgatgggaac tactgtgtgg ctgatagtga
     4681 cgtttgtgag ttattatttg acgtatctta cggacggcag ggccgtagcc ggcaggtata
     4741 cggttatgct tagactggct cagccgatat acacgcttgc cattgcaatc tgggctgtaa
     4801 tttatacgca tactgcccat acgtggcagc atggcgataa aaaattcgct atggaacgca
     4861 tggatgtgat gtttcgtcgt gtaatggcga tggttcttgt cggcggctgg gcaattattc
     4921 tgagcgagcc ggcctggtcg cgcatattag ggccgaaata ttgcggcgca ggcggatatc
     4981 tacccggcct gttgatgatg tttgccgcaa tgagcagcgt attgagcctt tttacttggg
     5041 gccgtatttt gggcagacag tggtcgatag taatagccgg agcggctgcg gtcgtgttga
     5101 acgtggcggc atattatgcg tttgaacggt tttttattac taaaatgttt tcgtcgctgt
     5161 ccgccggcgt gggcgtattt gctggttcgg tgatgatgac cgtcttttat tttacgcact
     5221 ggaaaataaa aatttcgatt cgcagtgccg tgatgcttct gctgcctgcg atatttttac
     5281 tgtcgaatgg aattgcaaat tctctttgcg cggcggcaat tactttggtg atgtgctact
     5341 ttgcttttac tcccgccgag cgtaaaagac tttttgactt aaaaaattac agcaatcaat
     5401 cgcggttatt ttaaaaaccg cttatgaatg ctataactca gtttcgctga cgggagtttc
     5461 agtgccgact ggtgacatta ttgtcgggac gtcgtcgagc agttcaagca aattcttttc
     5521 ccatgcggta tattcttcgg cataccctga tatttccgtt ttatatgctg cctggtcgta
     5581 gggcttgtcc gattctatca gacgtatagc cattttgccc atgcgccata tgcctatgcg
     5641 acgtaagccc agattctgtt ccatggcgtc gaaccatgcg gggatttccg acatgtattt
     5701 gtcaaaagtc ttttctttgc ctgtggatac cgtcattgta agcattgtcc gatagcgtgc
     5761 tgcatgtcgg acggacgcac ggttatcgag cggtttgatg tccttggctt taaggccatt
     5821 ggcacttttt gttattttat aggatactct ttgatcctcg gcgagggctt tgaagccttc
     5881 catcagaata tcgctgaaat ggaagaagac atcgccttcc ggattctcta tgaacccgta
     5941 gcctttggat ttatcatacc attttactct accgacaggc atttcagccc tccaggattt
     6001 tttgggtaag aaccgtgtcg ccttgagtcc tccgacaatc ttggcggcct catgtaccac
     6061 gaatctgttt tttaatcttc gctgttctgt aaaaaacgtg cctttctggg ccatattttc
     6121 aacaaaactg gttttaaaat actttcctgc agaattctgc ggatttgccc gcggtctctg
     6181 cagaatctat attttggaag ttttaattat agcaaaataa cttaccatgt aaacaaccct
     6241 ttgacgcgca gcctaaagcc gacggtgaga atcgaactca caaccccagc tttacgaaag
     6301 ctgtgctcta ccgttgagct acgtcggcaa actgcaggtc agtctcacaa agagccagaa
     6361 tactatcagt aatggattga aaatcaagcc aaaaatcaaa ttttgagatt tccggcacac
     6421 gcacttttaa tttagccgcc gttacttata atgtcattaa atcacatttt tttaaggaaa
     6481 ctaaatatga gagaaaaagt tcaggaagtc atcgaccaga tcaagcccat gcttcaagcc
     6541 gacggcggcg atatcgagct tgtggatgta aacgagaaaa caggtgtagt ctctgtacgg
     6601 ttacagggtg catgcagagg ttgccccggt gcgcgcatga ctcttaaaat gggcgtggaa
     6661 cggaaacttc agtccgagat acctggcgtt acggaagttt tagccgtcga ctgacctttg
     6721 ttggccgcgt gtaactattg cggttttaga acgatatgtc acttaatccc gacgtacagg
     6781 ctgatacgcc agccatgcgg cagtacagaa cgttcaagga gcagtatccg gattacgttc
     6841 tgctgtttcg catgggcgat ttttacgaga cgttttacga ggatgcgaaa atagcatccc
     6901 gcacgttggg ccttacgctg accagccgca gcaagggccc caatgccgtt ccgttagccg
     6961 gcataccgta tcatgcgttg gacaattacc tgggccgtct gattcaagcc ggctatcgcg
     7021 tcgctctgtg cgaacagata gaagacccca aagaagccaa aggcgtcgtc aaacgcgacg
     7081 ttgtaaggct gataacgccc ggcactctta ccgatgaaac actgctggaa cagcgggagg
     7141 gcaattatct tgccgccatt ttttccgtcg gcagaaaacc tcaaaactgc gactcgccgt
     7201 ctgtcggcct tgcgtgggtt gagctttcca gcggagcgtt ctgggccatg cttgctccgg
     7261 cgcagacggc gctggatgaa cttgtgcgca taaatcctgc cgaggtcatt tatcccgaag
     7321 gcagcgaata cgaccagccc aaatggcgcg accacgccaa gcaggtcatc ggctgtgcat
     7381 tttcttcgcg tgcgccgtgg gcgtttgata aaaattcatc cgttcagact ttgcacaatc
     7441 attttcatac cgctacgctt gccggattcg gcttcgatga atgggacgag tccgtctccg
     7501 ctgcgggagc gataatagaa tatttgaatg agacgcagaa aaccgcgatg ggccatattt
     7561 gtgaacttcg cagatttgag cgtcgcgact atatggctat cgacggcaat actttgcgat
     7621 gtcttgaagt tttgcggacg ctgcgcacca attcgcgaca gggcagcctg cttgcgtgta
     7681 tagaccgtac taagacaggt atgggtgcga ggctgcttga acggtggttg acgtttccgc
     7741 tagtctgcta ttcatcgata attgcgcggc aggatgcggt ggaatttttc atctcgcgtc
     7801 gaaaatcgct cgacgctctt cgcgaacttt tgggtgattg tgcgcagata gaccgcataa
     7861 cgaccaatat tgcgatggct cgcgtacggc cgcgcgaatt tgttgcactg ggccagagtc
     7921 tggctcttct gccgcgcgtg gcggaatatc tggacgaaga tgccgacaaa ctcgaactgc
     7981 tgacccgatt gcggccggcg atagaaaatc tggatgaacc gctcgcgctt attgaaacag
     8041 ccatcgaccc cgactgtccg aacgtggtac gtgacggcgg agtgattcgg aatggattta
     8101 atgaagagct tgaccggttg cgggctatcg accgcgacgg ccaatcatgg ctggcggagt
     8161 atcagcagca ggaatcccaa cgcacgggta ttccgctaaa agtcgggtat aacaaggttt
     8221 ttggatatta tattgaaatc actcataccc atcgcgacaa agttcctccg gaatatcatc
     8281 gcaagcaaac gctgaagaat gccgagcgat acataaccga tgaactcaaa cattatgaag
     8341 ccgaagttct caccgcctct gaacgtgcca aagaaatcga acaacagctt tatgagcagg
     8401 tgcgggtctc gctgttgccg tggatagaaa aacttcaacg tgcggctgaa tcgcttgcca
     8461 cgctggatgt gttgagcagc cttgcgcagg tggcgctgga acgcggatac atacggccgg
     8521 aaatcacgca ggataacgtg ctgaacattg tcggcggaaa acatccggta gtggcggaac
     8581 tgctgcggga gcaatttgtg cccaacgatg ttaaaatggg caacgtcaaa gaccgtctgc
     8641 tcatcataac cggcccgaat atggccggca agagtacgta tatccgccag gcggctcttt
     8701 taacactgat ggcgcagacg ggctgttata ttccggcgga aagcgcgatt atcggcattg
     8761 ccgacaggct gtttacccgc gtcggggcag ccgacgaact tgcccgaggt ttgtcgacgt
     8821 tcatgcttga aatggtcgag acggcaaata ttctgaacaa tgctacgcaa cgttcgcttg
     8881 taattctgga cgaagtcggc cgcggcacga gtacctgtga cggtttgagc ctggcttggg
     8941 ctatttgcga atatatcgca cttaacatca aatgtcgcac actcttcgcc acccattatc
     9001 atgaactgac cgaacttgaa gatcttatcg atggcgtaaa aaatctcaac atcgccgttc
     9061 gcgaatggaa gggcgacgtg atttttttac ataaaatcat ccccggcggg acggatcgca
     9121 gttacggcgt acatgtcgcg aaactcgccg gtgtgccgaa ggacgtcatc aaccgggcgc
     9181 aggcgattct tccgcagatg caggcgcaac ttgccgatgt actcgatatg cccgaccttg
     9241 ccgacaaagc ccgcgccgcg gctgctcaga tgaatctttt tgccgatcct gccgcgcgca
     9301 tcgccggtga aatcagacac gccgaccttg ataatatgac tccgatacag gcttacgaac
     9361 ttttgagaaa acttaaagat gaattatcct aatgttgttt tacgccgacg ttgccgtttc
     9421 atccgggtta tgttttaaat ttttttgtca tgagattctg gtcggttgcg gctggtttgt
     9481 tccaatccag gtgataaatt tctgcggcgc cgtagcctgg tatgacgaac gtatctattt
     9541 ttgtgccgaa atttcgacag acagcgtcta tgatattttt atttcgccat gcgggcattt
     9601 tgtccggcat gggttttaca ccgccgctgg tctggaaatc atcgcatagg cgcacagcgt
     9661 attcatatct gcgttcaaaa atatctttcg gtgtcacttc ccaattttta tccagcggaa
     9721 aataaccgta attaaattta tcagcgccga tttgcaggat gatgttgcca tacactttcg
     9781 agcgtgggcc ggttacggaa gcaattctat ccgtcagaat gtcgatatcg gaacaatgaa
     9841 atcgcaacat tacgaacatg cccgcgaaac atatcagcag atagctgcaa gctatcgcac
     9901 cggctagcca taccttccag tttttaaggc tgtgtttatc aaaaatttaa tgcgagtttt
     9961 ccaccatatt cgttgtatga accttgtgag caacattgcg aaaaatggcg acagcaacga
    10021 cagataaact gcggcggtat ttacggtcgt aaacggcagt accagcgcga aaactatcgg
    10081 gacagtcccg ataattctgt cggccggttt tgatttgata aatgcagcca ttagtgaaca
    10141 ggcgtaaaga atcattaacg gcaggccttt gggccattga aaatatctca gccatcggcc
    10201 gaattcactt tgcagaagat atgttaccga ccatgcctcg cgcaatttcc cgcctgtcat
    10261 ctgtgccgcg aatgaaacgt tcggattatt aattgccgcg gcaacataaa tcaggtaagg
    10321 cgataccgta atgatgaaac ttatcgccat taccagtact gctttaaaaa aactttcagg
    10381 tcgccgccaa agcgtcacgg aaacgaaaat gcatccgcaa agaacaaaac cgttcggatg
    10441 agatagcgaa gctatggcac tgcaaatacc ggctattatc ggccacgcca attttccacg
    10501 cccgatttta agcatcatca ttaagaacag catcgcgaat gcaagggcaa agatttcagg
    10561 ccttacccgc cttgcgatga tgaaaaacca cggatttacg gcaaccatta ttgcgcatag
    10621 aatcgctgcg cggcgtgtga acaatttgtg cataatcgca taagttgtac ataccgttat
    10681 tactgccatc aataccgatg gtaatcgtgc tggaataact ccgtagccga atattagaaa
    10741 agaaatgctg caaaacagcg gctgaatcag ctgattctgt aaaaaatgca cgttcgtgcc
    10801 gccccagccg tcaatcgcgc cgttgcgtat ggaaccttca cgcgcgatat tgtatcctat
    10861 ggaagcctcc cagctttcgt cgccgtaaaa tcgcggtacg gttttgagat acacgacgca
    10921 cagaaggagc tccagcaatg caagcattat gatatcgagc ctgaaatttt tttgactatt
    10981 aatcatcata tttgcttttc ctcaaaaaat tttcaagcgg acagcctttg caaaccggtt
    11041 ttgttttgca gtattttttc gcgacggcta cgagttgggc atgataatcg ttaaacaatt
    11101 cggtgtcatg cggcagattg tcttcgcaga gttttttcag gtcatgatag tcagcgtctt
    11161 tgtcgcacaa gccatgcctg gaaaaaactc ttcgggtata cgcgtcgatt acgaatgacg
    11221 ccttaccggc agcataaagt atgatactat cggcagtttc cggcccgatg ccgctgatgt
    11281 cgagcagttc gtttctcaaa attttctcag attgacggaa catgtcagcc agccggccat
    11341 gatgattttt tttgacgtgc gatgtgaaat tctgcaatcg ttttgtcttg atattaaaat
    11401 aaccggctgg ccggataagt tccgccagtt ctgccggcgg cattgcatct attttttgca
    11461 gcgagagaca tcggtttttt ttcaggttgg cgattgcttt ctcgacattc tgccagcttg
    11521 tgttttgcgt gagtattgcc ccgacgcaga tttcaaacgg cgtatcgccg ggccaccagt
    11581 gctgatggcc gaatcggcag aacatcaggc gatacaactt taaaaaatct ctggcgggag
    11641 tcatcgccgc gccccgtcag ttgttgatga aagata
//
LOCUS       DAONFI010000019        11476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6333_length_11476_cov_4.034410, whole genome shotgun sequence.
ACCESSION   DAONFI010000019 DAONFI010000000
VERSION     DAONFI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11476
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6333_length_11476_cov_4.034410"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLK08_01230"
     CDS             complement(<1..351)
                     /locus_tag="PLK08_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPS52149.1"
                     /translation="MSHDIENLDHNYHGPVAWMAGHSVAANLCMLLCLVGGFIAMQNI
                     KQEVFPDLTLDVVRVTVSYPGASPDEVRKSIVEAIENVVMGIDGVDEVTSSAEEGSAT
                     VDVEVLEGADVQKVY"
     gene            complement(353..1603)
                     /locus_tag="PLK08_01235"
     CDS             complement(353..1603)
                     /locus_tag="PLK08_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPS52150.1"
                     /translation="MSESRLVQAEKKIIRTVTPLAIIAAGIGVMIFFVENKPRAQRVK
                     PQSSVTLIKTVPLEPGKHQTIIEGMGTVEPSKSISLIPQVAGRVEWISSNMIPGGRFK
                     KGEVMLQIEKVDYQLIVEQRKSEVSSAEKDLRLEVGQQAIAKREYEMLKQSITEDDKD
                     LVLREPYMKYARANLAAAKAALEDAELNLKRTTMTAPFNCSVESESVDPGSQLAVGSV
                     IAQLAGTDDFWIETSVPVDQLQWLNLNGGETQPKSYIYNLTAWGKDAFRVGYVKNMMM
                     HLETEGRMARLLVNVVDPLCMRKENHGMPRLILGMYVRVNIEGKTLTNVYRIDRGALH
                     DGDRIYIMTKNDKLEIRTVDIVWRGNQYVFVEKGITPGERLIVTDMPVAVEGMSLREA
                     PDDEPADSQPSTQSASQPAAKEAD"
     gene            complement(1646..3049)
                     /locus_tag="PLK08_01240"
     CDS             complement(1646..3049)
                     /locus_tag="PLK08_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPS52151.1"
                     /translation="MAKRFLLMILGGLVFLAGCKAPKSADNVVVPPQKFSESGHAPML
                     DKWWRSFGDENLNTLVEESLQGNFSLKTAADRLTQYKAQAIIAGADLYPQVQGEGNAT
                     RYRYEDRGGRYYESDYGLGVVASYEIDLWGRVRNTAEAAKLDVSASEDDLKTAAITLV
                     ASVSEAYFGLIESRGQLVLLDEQIKTNQDYLELVTLRFRRGQVSVTDVLQQKQLLEST
                     IGDRKKMQATVKVYENQIAVLTGKSPGDYSVRTAGDLPILPALPVTPVPSELLQRRPD
                     VHSTFLAVQAADKRVAVAIADCLPSFSLTISAGGYGTSTNELFSQWLGTLGGNMVAPL
                     FEGGKLLAEVERRKAMASEAVNSYAHTMLTALREVEDALVQETQQEKFLESMKEQLKL
                     SMQSVDQTRERYTKGAMDFLRFLTTVSNNQELQRTYLTSRRQLISYRISLYKALSGGW
                     DVTPLEKQADMNIRLLN"
     gene            complement(3062..3691)
                     /locus_tag="PLK08_01245"
     CDS             complement(3062..3691)
                     /locus_tag="PLK08_01245"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPS52152.1"
                     /translation="MSISSDTKHKLLDAACDIFAEKGYRNATIKDICGRAEANIASVN
                     YYFGSKKVLFEFVWRQVYEIINEKYPLEEGVGPNPTPQERLRCHIYALMRRIFDEGRV
                     GLLHAMTFSAGSHDVETMAIIKELFRSHRDYMAGNVRAILGSAVPDMYVKLCSMSIVS
                     QCFSLYRWPLPDGKKFRDIFDMGPEVMAGHIYRFSLAGLESTRKMFEKQ"
     gene            complement(4091..5212)
                     /locus_tag="PLK08_01250"
     CDS             complement(4091..5212)
                     /locus_tag="PLK08_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPS52153.1"
                     /translation="MSATIFKFFKFFKNFLKKLEEINPFNGIIAGLLIIGLLIVGWDW
                     SAARKSVKQRCFATAALSAIMSTPQFNMAVCAPTDHCAAEAVKRAEDAARIVEREMNI
                     YNPASELFKFNAAKPGSIVNLSAETQTAIRLARKAFDETGGAFDATLRPVILLWKNAA
                     KTNKLPAVAELAAARKSSSWSDFRFSDGQIGKMQQSACVDLGGIAKGFAIDLAVGAMK
                     KAGCEGGLVDIGGDVGCFGHKNDGSEWVIGIRDPFEPHSGKMLATLHLTDAAVCTSGN
                     YERAELICGRQYNHIIDPRTLQPADTCPSVTVIAPTAAMADAWATALSVLGPDGLKLL
                     KKNGNIHAMLVVGKRNNFNIVTSPDFSRKFVTQIEGVLN"
     gene            complement(5251..5604)
                     /gene="queF"
                     /locus_tag="PLK08_01255"
     CDS             complement(5251..5604)
                     /gene="queF"
                     /locus_tag="PLK08_01255"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPS52154.1"
                     /translation="MSDKLDILDVFDNPHPGRDYIIKHVAPEFTSLCPKTGQPDYGTI
                     TVEYIADKTCVELKSLKFYYQSFRNEGVFYEDLTNRILDDLVKKLAPRWMRITAEFTP
                     RGGLHSVITAEHGNR"
     gene            5720..6433
                     /gene="queC"
                     /locus_tag="PLK08_01260"
     CDS             5720..6433
                     /gene="queC"
                     /locus_tag="PLK08_01260"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPS52155.1"
                     /translation="MSEKNEHNSPLAVVLLSGGLDSATTLAISHSEGFTCHAISFDYG
                     QRHKFELRQAALVAQSQAAASHRIIRIDLSAAGGFGASALTDNIAVPKDRHDTSHGSI
                     PVTYVPARNTIFLSFALGYAEVIGAFDIFLGVNAVDYSGYPDCRPAFITAFEKLANLA
                     TAAAVEGRGQYRIHAPLINLTKAQIIRKGLELGVDYSITHSCYSPSADGRACGHCDAC
                     VLRREGFATAGVNDPTKYA"
     gene            6638..9202
                     /locus_tag="PLK08_01265"
     CDS             6638..9202
                     /locus_tag="PLK08_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HPS52156.1"
                     /translation="MSGLKNIKRMFVAGMAVLLMLAYVNVALAAVSITGEVTPSDIDT
                     DNDQPSVDLYVGSATNFGQLDIDGGSTVTVNGANVGYGTNDGTINIDGGNMVTNSDMT
                     VGESGLGSLFLDNSSSLTVNGWLYLAGDYAGNTTTIMNGSTLTVTDTTSGLYIGGGAN
                     TVTFTASNSGTITVGKLEIHDNANVSFTGTSTLNITGDATFEGDYDVEFQQDVAIGGD
                     MTVSGDSTSSLTVVGTGTTLDVGGTLTIDSTSGITVQNSAVVSTDVLEITSGSTLVVD
                     DGVSSGAVVVGTGSAVNGSVVVASDGILRNSGTVEGQVEIENGGQIGNEGDDPNQITI
                     ASGYDFTLASGSTYVVNIDTTKSPANDNISIVSGNATLESGSVIKVVPVSLSQLSAGD
                     TFDVITASSGTITDNGVTIEQASWASLFRYFTGSVVGGNVYQLLYTTSSFQTEAHGRN
                     NVSVAAALDDMLNSGTATAAQTTMLDNLLSFTDNASFNNALHQMNAEIYTLHGRNLPN
                     QMESFNRFISQHLTQMRSGKVAQLTAKADASMGMALASTEVDPETMAEVIESQKEQDQ
                     RQDAITDPDAVSSTDWSVWGQIINMWHKEKSSSRLTGYNNYSWGAVAGTDKEFAPGLR
                     IGALIGYIRSDYHMKDSRGKLDVDTLRVGPYVDWTRGKMFVEAALTFGYHWNDAERKV
                     NYTGFSDEDKADNYGAWDLTPYARVGYRFNVAGWDVTPSMAMQYVHYSQNGVTEHGGA
                     AAMKVEDYDTNSLRQIFMVEVSKLFDLNYVKLVPALTLGYSYDYLDTGETLGSEFIGG
                     YPCTFYPGGTSKGHVIWGASVTALLSDNLSLFVRYDGFAAGSDYSNALSSGVNYKF"
     gene            complement(9444..9812)
                     /gene="rnpB"
                     /locus_tag="PLK08_01270"
     ncRNA           complement(9444..9812)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLK08_01270"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9927..11342
                     /gene="mgtE"
                     /locus_tag="PLK08_01275"
     CDS             9927..11342
                     /gene="mgtE"
                     /locus_tag="PLK08_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPS52157.1"
                     /translation="MGERYAWIQQNNPERDGMENEVQTFDIEQVHELVEGRKFGQLKA
                     ALSAMEIHELGDLLSRIENENELAIAFRMLPTEKAAEILAHLESDQQEDLLHKLSKEK
                     VAEIINEMAPDDRTELLEEMPGQLAQKLLMQLRGDERKIADSLLAYPEDSIGRLMTPK
                     YLAVRKDWTIDHVLRHIRNRAEEAETVKMIFVVDRKWRLVGSIDIEQVVLSDPAENVE
                     EVMSEQVGCLLATDDQESAVDAFKKYDAIALPVVDSWNTLVGIVTFDDVMDLQEEEST
                     EDMQKMAGMAALEDSYFTADFLHMLKKRLPWLVLLLFVETLAVMALKSYESLLALLAM
                     FTPLINATAGNTGNQVAGLMIRGFAVKEIELSHWYRVFFREVVRGAVMGATLGLVSCG
                     IVLVFQHTPDRWKVAWAVAMAMLVAVVMANVIGSMLPFIFKRFGIDPAITSGPFIAGL
                     MDVSSIVIFFAIAHNAMNIIS"
BASE COUNT         2738 a         2916 c         2897 g         2925 t
ORIGIN      
        1 atagactttc tggacgtcgg ctccttcgag aacttccacg tcgacagtag ctgagccttc
       61 ctccgccgat gaagtcacct cgtctacgcc gtctatgccc ataacgacgt tttcgattgc
      121 ttcgacgatg cttttacgga cttcgtcagg actggcaccc ggatacgaca ctgttacccg
      181 tacgacatcc agcgtcaggt caggaaagac ctcctgcttg atattctgca ttgcgataaa
      241 tccgccgacc aggcacagca gcatgcaaag attcgcagcc acgctatggc cggccatcca
      301 cgcaacgggg ccgtgatagt tatggtcgag attttcgata tcgtggctca tatcagtcag
      361 cctcttttgc agccggctga gatgcggact gagttgacgg ctgactgtcg gccggctcgt
      421 cgtctggagc ttccctgagc gacatgcctt cgaccgcgac cggcatatcc gtgacgataa
      481 gtctctcgcc gggcgttatg cctttttcaa cgaacacgta ctggttgccg cgccagacta
      541 tatcgaccgt acgtatttcc agcttgtcgt ttttggtcat aatatatatt ctgtcgccgt
      601 catgcagtgc gccgcgatct atacggtaga cgtttgtcag cgtctttcct tctatgttta
      661 cgcggacgta catgcccaga atcagcctgg gcatgccgtg attttccttc cgcatgcaca
      721 gcgggtccac cacattcacg agcagcctgg ccattcggcc ttccgtctcc aggtgcatca
      781 tcatgttttt tacgtatcct accctgaatg cgtcctttcc ccacgccgtc aaattgtaaa
      841 tatacgactt gggttgagtc tctccgccgt tgagattcag ccattgaagc tggtcgaccg
      901 gcacggacgt ttcaatccag aaatcatccg tacctgcaag ttgcgctatt acagagccga
      961 ctgctaactg cgacccgggg tctacggatt cactctcgac gctgcaatta aaaggagctg
     1021 tcatggttgt ccgtttaagg ttcagctcgg cgtcttccag ggcggctttg gcggcggcga
     1081 gattggcccg ggcgtatttc atgtacggct cccgaaggac gaggtctttg tcgtcttcgg
     1141 ttatggactg cttgagcatt tcatattcgc gttttgcgat agcctgctgt ccgacttcca
     1201 gccgcaggtc tttttcagct gaactgacct cgcttttacg ctgttcgaca atcaactggt
     1261 aatcgacctt ttcaatctgg agcatgactt cgcctttttt gaaccgtccg ccaggaatca
     1321 tgttggacga tatccactcc actctgccgg cgacctgcgg aataaggctg atgctcttcg
     1381 acggttcgac cgtacccatg ccttcgataa tggtctgatg tttgccgggc tcaagcggca
     1441 cggttttaat cagcgttacc gacgattggg gcttgacccg ctgtgcgcga ggtttgtttt
     1501 ccacaaaaaa aatcattacg ccgatgccgg cggctattat cgccagcggc gtgacggttc
     1561 ttatgatttt cttttctgcc tgtacgagtc tgctttcact catatgtgcc atacctcttc
     1621 atttcaacat catgtgaata aggcatcagt tcaacagtct gatattcatg tccgcctgtt
     1681 tttcaagcgg tgtaacatcc cagccgccgg aaagagcctt gtaaagcgat attcgatacg
     1741 aaataagctg cctgcgtgac gtaaggtaag tacgctgcaa ttcctgattg ttgcttaccg
     1801 tagtcagaaa tcgcagaaaa tccattgcgc ctttggtgta tcgttcccgc gtctggtcga
     1861 cggactgcat ggaaagtttc agctgttcct tcatgctttc cagaaatttt tcctgctgcg
     1921 tttcctggac gagtgcgtct tccacttctc gcagtgcagt gagcatggta tgcgcgtagc
     1981 tgttgacagc ctccgaggcc atcgcctttc gcctctcaac ctcagccagc agcttaccgc
     2041 cttcaaacag cggcgccacc atattcccgc ccagtgtacc taaccattgt gaaaaaagtt
     2101 cgttcgtact cgttccgtaa ccgccagccg atatcgtcag gctgaaactc ggcagacagt
     2161 ctgctattgc aacagcgacg cgtttgtcgg cagcctggac ggcaagaaac gtgctgtgga
     2221 cgtccggccg ccgctgaagc agctcggaag gcacaggcgt aaccggcaga gccggcagaa
     2281 ttggcaaatc gcccgccgtt ctgacgctat aatcgccagg acttttaccc gtaagaaccg
     2341 caatctgatt ttcgtagacc ttgacggtag cctgcatttt ttttctgtcg ccaatcgtcg
     2401 attccagcag ctgtttctgc tgcaacacgt cggtgaccga cacctggccg cgccggaatc
     2461 gcagcgtgac gagctcaaga taatcctggt tcgtttttat ctgttcatcc aacagtacga
     2521 gttggcctct gctttcgata aggccgaaat aagcctccga aaccgacgca accagcgtta
     2581 tcgccgcggt tttaaggtcg tcctcgctcg cagatacgtc cagtttggca gcttcagcgg
     2641 tgtttcttac gcggccccac aaatctattt catacgacgc cacgacgccg aggccataat
     2701 cgctttcgta ataacggccg cctcggtctt catagcgata ccgagtcgca ttgccttcgc
     2761 cctgtacctg cggatagagg tcggcgccgg caataatggc ttgggcttta tactgggtca
     2821 gacggtcagc cgcggtttta agcgaaaaat tgccttgcag cgattcttca accagcgtat
     2881 tgagattttc gtcgccgaac gaacgccacc acttgtccag catgggcgcg tggccgctct
     2941 cggaaaattt ctgcggcggc acgactacgt tatcagccga cttcggagct ttacagccgg
     3001 ctaaaaatac cagtccgcca agaatcatca gcaaaaatct ttttgccata atcgacgccc
     3061 tttactgctt ttcaaacatc tttctggtac tttcaaggcc agccagcgaa aagcgataaa
     3121 tatgtccagc catcacttcc ggccccatat cgaaaatatc cctgaacttt ttgccgtccg
     3181 gcaacggcca gcgataaagg ctgaaacatt ggctgactat gctcatactg cacaatttga
     3241 catacatatc aggcacggca gagcccagta tcgcacgaac gttgccggcc atgtaatcgc
     3301 gatgcgacct gaaaagttcc ttgatgatgg ccatcgtctc tacatcgtgt gagccggctg
     3361 aaaacgtcat ggcgtgaagc aggccgacgc ggccttcgtc aaaaatccgc cgcatcagcg
     3421 catatatgtg acaccgcaga cgctcctgcg gcgtgggatt cggcccaact ccctcttcaa
     3481 gaggatattt ctcgtttatt atctcgtaca cctgccgcca aacgaattca aacaacactt
     3541 tcttgctgcc gaaataataa ttcaccgatg ctatattagc ttcggcacgg ccgcagatgt
     3601 ccttgattgt cgcattgcga taaccctttt cggcaaaaat atcgcaagcg gcatcaagaa
     3661 gtttatgttt tgtatcactc gatattgaca tcttgaattt cacgccatca ggctatgtcg
     3721 tcagaacggc tcacaaaaag aaacctcttt tgcaaaccac tctaaaagct tgaaaacagg
     3781 acaataaaaa taaatcaata atcaaaacgc atattttaaa cgttcatttt aaatatgcaa
     3841 ttgttttttc tttttttatt agacgtggtt tgcgaaagat ttttttagaa tcgctctatt
     3901 tgttgctatc tttagagggg taagctattt gccgttctca attaccctgc ggccgcaaaa
     3961 aataagatat taaaggctgt gagcggcaat catacatctg cgacgtcaca aaaactcagc
     4021 gaattgacag attcgcagtc gtttttgttc cttgcatctg cataattgcc actcatttgc
     4081 gtctcacagt ttaattgaga acgccctcta tttgcgtcac gaattttcgg gaaaaatccg
     4141 gcgatgtgac gatattgaaa ttgtttcttt tgccgactac tagcatggcg tggatatttc
     4201 cgtttttttt cagcagtttc aagccgtccg gcccaagcac actcaacgcc gtcgcccacg
     4261 catccgccat cgccgccgtc ggtgcgatta ccgtaaccga gggacaagta tcggccggct
     4321 gtagcgttct tgggtcgatg atatgattat attgtctgcc gcaaatgagt tccgcccgtt
     4381 cgtagttgcc gctggtgcag acggcggcat cggtcagatg tagcgtggcg agcattttcc
     4441 cgctatgcgg ttcgaacgga tcacgtatgc cgataaccca ttcactgccg tcgtttttgt
     4501 ggccgaaaca gccgacgtct ccgccgatat ccaccaggcc gccttcgcag ccggcttttt
     4561 tcatcgcccc gacagccagg tctatggcaa aacctttggc tatgccgccg agatcgacgc
     4621 aggcggattg ctgcatcttg ccgatttggc cgtccgaaaa tctgaaatcg ctccacgacg
     4681 agctttttct ggcggcagca agttcagcca ccgccggcaa tttgttggtt ttggctgcat
     4741 ttttccacag cagtatgacg gggcgaagag ttgcgtcgaa tgcaccgccg gtttcatcga
     4801 aggcttttcg agccagccgt atggccgtct gcgtttctgc cgagagattt acgatagaac
     4861 cgggtttggc tgcgttgaat ttaaaaagtt cgctggcagg gttgtaaatg ttcatttccc
     4921 gttcgacgat tctggcggcg tcttcagcac gtttgacggc ctcggcggca cagtggtcgg
     4981 ttggggcaca gacggccatg ttgaactgcg gcgtggacat tatcgccgac aatgccgccg
     5041 tagcgaaaca tctttgtttg acgctttttc tggcggctga ccaatcccat ccgacgatca
     5101 gaaggccaat tatcagaagg cccgcgatta tgccattgaa aggattgatt tcctccaatt
     5161 tttttaaaaa atttttaaaa aatttaaaaa atttgaatat tgtggctgac atttactgtt
     5221 tctcgaatcc acaagtttcg gaattttcga ttacctgttt ccgtgttcag ccgtgatgac
     5281 ggaatgcaag ccgccgcgcg gcgtgaattc agccgttatt ctcatccatc gcggagcgag
     5341 tttttttacc aggtcgtcaa gaatgcggtt ggtaaggtct tcatagaaaa cgccttcatt
     5401 acggaagctc tggtagtaga atttgaggct tttgagttcc acgcaggttt tgtcggcgat
     5461 gtattccacc gttatcgtgc cgtagtccgg ttggccggtc ttcggacaaa ggctggtgaa
     5521 ttccggcgcg acgtgcttta ttatgtagtc acggccagga tgcggattgt caaaaacgtc
     5581 cagaatgtca agtttatcac tcattttctc aaaatccttg cgaaggtttc agattttaaa
     5641 gtcggcgtct gccgttaaaa ggaatactat acatttaaat gcgttgcata taaagaggcg
     5701 aaagtgtaat ctttgcggca tgagtgaaaa aaatgaacat aatagcccgc tggcggtggt
     5761 gttgctgtcc ggcggattgg attcggctac gacgctggcg atcagccaca gcgagggttt
     5821 tacctgccac gccataagtt tcgattatgg ccagcgacac aaatttgaac ttcgccaggc
     5881 agctctggtc gcccaaagtc aagccgccgc cagccaccgc atcatcagga tagacctttc
     5941 agcagccggc ggtttcggcg ccagcgccct cacggacaac atcgccgtgc ccaaagaccg
     6001 gcacgataca tcgcacggct caatacccgt aacatatgtg cctgccagga ataccatttt
     6061 tttatcgttc gcgttgggct atgcggaggt aatcggggca ttcgacatat ttttgggtgt
     6121 aaatgccgtt gattacagcg gttatcccga ttgcaggcct gcgtttataa cggcttttga
     6181 aaagctggcg aatctggcta ccgcggcagc tgtcgagggc agaggccaat acaggataca
     6241 tgctccgctt ataaatctga cgaaagccca gattatccgc aaaggcctgg aactgggcgt
     6301 ggattattca attacgcaca gctgttattc gccgtcggct gacggccgag cctgcggcca
     6361 ttgcgacgcc tgcgtgcttc ggcgggaagg gttcgcaacc gccggcgtaa atgacccgac
     6421 aaaatatgca tgaaaaccgt ttttattaaa gattctttcg ctcaaatacc gaaaaaatga
     6481 tatgaaaata taggttttag cttgaattga aaaagtatct tttgtataaa attgccagtt
     6541 cggaagagtt atgcgcacat agagcgcaac agaattttaa ttagttgatt attaataagg
     6601 gctggtcgaa gcaaggcggc ttcgggaggt ttttataatg agtggtttaa aaaatatcaa
     6661 acgcatgttt gttgcaggca tggcggtgtt gttaatgctg gcatatgtta atgtcgcatt
     6721 ggctgcagtg tccataacag gcgaagttac tccgagtgat attgacaccg ataacgatca
     6781 gcccagcgtc gatttgtatg tcggcagtgc aaccaacttc ggccaacttg atattgacgg
     6841 cggctcgacc gttaccgtca atggcgccaa cgtcggctac ggcactaatg acggcactat
     6901 taatatcgac ggcggcaata tggtgaccaa tagcgatatg accgtcggcg agagcggctt
     6961 ggggtcactg ttccttgata acagttcatc gttgacggtc aacggctggc tgtatctggc
     7021 gggcgactac gccggcaaca ccacaaccat aatgaacggt tcgacgctga ctgttacgga
     7081 tacgacatca ggcctttata tcggcggcgg cgctaacacc gtcacattta cggccagcaa
     7141 tagcggtacg attactgtcg gcaagctgga aatacacgac aatgccaacg tgagcttcac
     7201 cggcacttca actttgaata ttaccggcga tgccacattc gaaggcgatt atgatgtcga
     7261 attccagcag gatgtcgcta tcggcggcga tatgaccgtt tccggcgata gtacatcatc
     7321 gctgacggta gtcggtacag gaacgacgct tgatgtaggc ggtacgctga cgattgattc
     7381 tacaagcggc ataactgttc agaacagtgc tgtcgtaagc acggatgtac tggaaataac
     7441 tagcggcagc acactggtcg tggacgacgg cgtaagttcc ggtgctgtcg tagttggcac
     7501 aggttcggcg gttaacggtt ctgttgtggt tgcgtcagac ggtatattgc gaaactcggg
     7561 cacagtagaa ggccaggtcg aaattgaaaa cggcggccaa atcggcaatg aaggcgatga
     7621 cccgaaccag ataacgatag catccggtta tgattttact ttggcaagcg gctctactta
     7681 tgtagtgaat atcgacacga caaaatcgcc cgctaatgat aatatctcca tcgtatccgg
     7741 caatgccact cttgaaagcg gttcagtcat aaaagttgta cctgtctctt tgagccagtt
     7801 gagtgcaggc gatacttttg atgtgataac tgcttcaagt ggcacgatta cggataatgg
     7861 cgtcactatc gagcaggcgt catgggcttc tctgtttcga tattttaccg gctctgtcgt
     7921 cggcggaaac gtttatcaac tgctttatac taccagttca tttcagacag aggctcacgg
     7981 aagaaacaat gtttccgttg ccgcagccct cgatgacatg ttgaattcag gtacggcgac
     8041 ggctgcccag actacaatgt tggataacct gctctccttt accgacaatg cctcgttcaa
     8101 taatgcattg catcagatga atgccgaaat ttacacactg catggccgca atctgcctaa
     8161 ccagatggaa agttttaacc ggtttatcag ccagcatctc acccagatgc gcagcggcaa
     8221 agttgcacag ttgaccgcca aggctgatgc cagcatgggt atggcgttgg ccagcactga
     8281 agttgatccg gaaaccatgg ctgaagtcat tgaaagccag aaggaacagg accagcggca
     8341 ggatgcgatt acagatcctg atgcagtcag cagtaccgat tggagcgttt ggggccagat
     8401 aataaacatg tggcacaagg aaaaatcctc atcgcggctg accggatata ataactactc
     8461 atggggtgcc gtggccggaa cggataagga attcgccccc ggcttgcgca taggtgcctt
     8521 gataggctat attcgtagcg attatcacat gaaagattcc cgcggcaaac tggacgttga
     8581 tactctgcga gtcggcccat atgtcgactg gacgcgcggc aagatgttcg ttgaagccgc
     8641 acttacgttc ggctatcact ggaatgacgc ggaacgcaaa gtcaactaca ccggtttcag
     8701 cgacgaagac aaagccgaca attacggcgc atgggatttg acgccctacg cccgcgtcgg
     8761 atacaggttt aatgtggccg gctgggatgt gacgcccagc atggcgatgc agtacgtaca
     8821 ttacagccag aacggcgtga cggaacatgg cggcgcggcg gcgatgaaag tcgaagatta
     8881 tgacaccaat tcgctccggc agatattcat ggttgaagtt tcgaaactct ttgacctgaa
     8941 ctatgtaaaa cttgtgccgg cgttgactct tggttattct tacgattatc tggataccgg
     9001 cgaaactctg ggttcggaat tcatcggcgg atatccatgt acgttttacc ctggcggcac
     9061 atcaaaaggc catgtcatat ggggcgcaag cgtgacagca ttgcttagcg acaacctgtc
     9121 cctgttcgtt cgttacgacg gcttcgcagc cggcagcgat tacagcaacg ctctgagcag
     9181 tggcgtcaat tacaagttct gatataaaac gataattagt ggtacatgaa ggctggcttt
     9241 atcgaggcca gccttctttt ttagcagttt ttgaaaagaa cctctctaat atcaaaatat
     9301 tcagaaatga aatccttatt tttctaagga taatagcgcg gcatattttt taaaaaaaat
     9361 attctgccgt ttttctgacg ccaccaggcg gcaaaagagc cgattacagg cagccaagat
     9421 ttggggatag gcctaattaa taaaaggctg gatgcgctat aagccgaatt ctgtaccctt
     9481 ttcagggtga aggtcattta tctgggccgg catttgcata caggttccgg cgttagcgcc
     9541 gagcggccta ccagcgacat attgcccgga caaagcagtc gcaatttggc cttgcacccg
     9601 gtggggtttg ccgtgccgtc gccatcactg acgacgcggt gcgctcttgc cgcacctttt
     9661 cacccttacc gacataagtc ggcggtctgt tttctgtggc actttcccta tgttaaaaaa
     9721 aacacggtgg gcgttaccca ccaccgttgt ccggaggtgt tcggactttc ctcccccatc
     9781 ctcaggatgg gagcgacctt ccgcgcatcc agccgcaaaa attataccag attgcaccga
     9841 atataagcct atattttaag acatcatgtc tatttttctt aaaggttgac ttttgtggcg
     9901 gatatggcga aaataaccca gcaattttgg gggaacggta cgcatggata caacagaaca
     9961 atccggaacg cgacggcatg gaaaacgaag tgcagacatt cgacatcgaa caggtgcacg
    10021 agcttgtaga gggacgcaag ttcgggcagt tgaaagctgc gctgagtgca atggaaattc
    10081 atgaactggg cgacctgctc agccgcattg aaaacgagaa tgaactcgca atcgcattcc
    10141 ggatgcttcc cacggaaaaa gccgccgaaa ttctcgcaca tcttgaaagt gaccagcagg
    10201 aagacctgct ccacaaactt tcaaaagaaa aagtggcgga aatcattaac gagatggctc
    10261 cggatgaccg tactgaactt ctggaggaaa tgcccggcca gcttgcccag aaacttctca
    10321 tgcaactccg cggcgacgaa cgtaaaatcg ccgacagcct cctggcatat cctgaagatt
    10381 ccatcggcag actgatgacg cccaagtatc ttgccgttcg caaggactgg acgatagacc
    10441 acgttttgcg gcacatacgc aatcgtgccg aggaagctga aacggtcaag atgatattcg
    10501 tggttgaccg caaatggcgg cttgtcgggt ctatcgatat tgagcaggtc gttttgtccg
    10561 acccggcgga gaatgttgaa gaggtaatga gcgaacaggt tggctgtctg ctggctaccg
    10621 acgaccagga atcggcggtt gacgctttca aaaagtacga cgccattgcc ctgccggtcg
    10681 tggatagttg gaatacactt gtcggcattg tcacgttcga tgacgtcatg gatttgcagg
    10741 aagaggaatc cactgaggac atgcagaaga tggccggcat ggcggctctg gaagacagct
    10801 actttaccgc cgattttctg catatgctca aaaagcgact tccgtggctg gtattgttgt
    10861 tgttcgttga aacgttggct gtcatggcgc tcaagagtta cgagtcgctg ctggcgctgc
    10921 tggcgatgtt tacgccgctc ataaacgcca ccgccggcaa taccggcaac caggttgcag
    10981 gattgatgat tcgcggcttt gccgtcaagg aaatcgaact ttcacattgg tatcgggtgt
    11041 ttttccgtga agtggtgcgc ggcgccgtca tgggagcaac gcttggcctt gtgtcatgcg
    11101 gaatagtgct ggtttttcaa catacgccag acagatggaa agtcgcgtgg gcggtagcca
    11161 tggcgatgct ggtcgcggtt gtaatggcta acgtaatagg ttcaatgctg ccgttcattt
    11221 tcaaacgttt cggcatagat ccggctataa ccagcgggcc gttcatcgct ggccttatgg
    11281 acgtttcaag tatcgtcata ttctttgcca tcgctcataa cgccatgaat atcatctcgt
    11341 aaaaatgtat atcgtcttct gccgcgattt tttaaaaaaa tcagaaaaat cagtacaatt
    11401 tcagtctgca ccaaagataa gtagaaccta tcccaaaaac atgggcgtcg ccgaaatcga
    11461 gcaattttgc ggcccg
//
LOCUS       DAONFI010000020        11474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6335_length_11474_cov_3.344951, whole genome shotgun sequence.
ACCESSION   DAONFI010000020 DAONFI010000000
VERSION     DAONFI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6335_length_11474_cov_3.344951"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="PLK08_01280"
     CDS             <1..1935
                     /locus_tag="PLK08_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52158.1"
                     /translation="KYEWTPGDDIWDKYFRGKFVSEYEFPVKVENNRLDIDISMPQNG
                     DNEKNKLLSRQVCTVNGMLIYPAAKAEELKATLESINKERKQQFEKQALLNIAPQNGT
                     MPAISKEYQESGYVPFVRNFCKDLYHNSLPVEDEIGKPVTISAAIGERAISRFAIRPL
                     CNLKNITLEISDLVSDNGMKLPPACVSWHWLRYIEQPVDKYKFRTLDYRPIADLIMPA
                     GSSDAVKDYNRQFLVEVTPSKAVAAGVYNGTITIKPANAPAVKLALKVVVYPFKLETY
                     ANDDERIQIYDPKSFLCHYGNLWLTSDDKEFWEYVDKDLSLMKKYRMAPTVMFDWFAS
                     MNDLDRFMDIYMKYGFHGYPLFGGYELLRIFDGKKPENTGISPFIEVLKQVTSRQKEK
                     HWPPFLFYCTAEIHTGMPAYIKAKGFLKQMKNAVPDAKLLVLINKNDEMDMLVNSDAD
                     VIGPNAVSMTEDGIEKVRQAKKKLWFYGWGRERFRCGLIDWRIRNRGGVSEWYSFVAQ
                     APFNPFDGKYPDCWNDAPPYLGPSGPIPTMNLEIFTAGRIDFLYLATLEQWLKRAGKI
                     KSPQSIQACAKAQKIIDELNETIVPDYYYYYKRRKLAMNAMSPLDIPRKKIFGFADED
                     YDKLRQRIAESICELKDACK"
     gene            1922..2905
                     /locus_tag="PLK08_01285"
     CDS             1922..2905
                     /locus_tag="PLK08_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52159.1"
                     /translation="MPVSKALILLLMFLPCAAGTDACKTVKLTDDEYDNHSPVWSPNG
                     KKIAYVSIRGRKKCLVLRPAACSKDEKILAENIADTPVISWQPDGRFIAVTACLKNEK
                     LVYVINTVTGQCRVFPGTFCKYSPNGKILAVIQNGRIALLNSVTGKVDKKFKVPVNVQ
                     GFDWSPDGKIIFYSCDGDLWKMDIDGSSIVRLLNHTKGSKGVTSPFIESPKVYLGGKK
                     VIFSLIVDGLYAGTTHNMTGSYDIANASFAVLGDADGWDVSPCGRWIVMAVGSNLMLY
                     DLDKNKTVMIGNGSEPSFSPDGRRIAYSHSKELFAPGEIFIMEMPESCFKK"
     gene            3025..3933
                     /locus_tag="PLK08_01290"
     CDS             3025..3933
                     /locus_tag="PLK08_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPS52160.1"
                     /translation="MYMQEIEFTSTADGSHQKALFYVPGPKAKRPLPLVVGLHQWSVH
                     LDERRNGPAYFSQCRKRGWAFIYPEFRGPNDRPQACASLLAVQDVIDAVDYAKSHAAI
                     DEDRIYMVGASGGGMMSLIMAGRYPEIWAAVSVWVPIFDLKKWYADGMKRGNRYPSML
                     EACCGGAPGSSRGVDGQYRIRSPKSCIKNAANLSLDINAGVHDGHIGAVPIYHSFDAF
                     NVLAKAAGFDEKIVGTADIKYMVTQEAIPPALRYKKRESFNRMHKVLFRRQAGKVRIT
                     IFEGGHEVDPTAAFEFFVGKKRSSRE"
     gene            complement(4115..4258)
                     /locus_tag="PLK08_01295"
     CDS             complement(4115..4258)
                     /locus_tag="PLK08_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52161.1"
                     /translation="MKVYLAIKELIEEFQLDALTVGCYPDLMDRVCMAASLLADDGIP
                     LAC"
     gene            complement(4387..4956)
                     /locus_tag="PLK08_01300"
     CDS             complement(4387..4956)
                     /locus_tag="PLK08_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52162.1"
                     /translation="MSIIKNKLIPSIAVGALSSRLEVGTDRASHAAGQAAEILAELGM
                     TVENLGSIDTPQKAVSARISVSQKRVDAIVLVAVSWFEDYLVLDLLEECSRPLLLWSL
                     PGMETGALCGVQQLTAYLKHLEVPYGCIYSQLESVESQQKAKDFLLGTALKSRLRRTK
                     IGLDGYHVRGMTEVSVNEFALKKQLGLGL"
     gene            complement(4964..6202)
                     /locus_tag="PLK08_01305"
     CDS             complement(4964..6202)
                     /locus_tag="PLK08_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS52163.1"
                     /translation="MTSKERILAALNGEPVDHIPLTTWTFGFPAPEHLKWQTNGKPVD
                     YWYTLRLEHLHNLPLPWTLEDEFKRCEAWASLGLDPYLEISVPWSQSKDVTFEDSIIP
                     IGEPGGDDKYPVMVRTYQTPAGPLRHAVRRTGDEGPGWPLQPNCVPIIEDYNIPRAVE
                     HAVSSPEDVDKIKYLFAPPSEGDKVWFEGRMKQIKKFADEKGFFTQAWTAFGMDAAVW
                     FTGTENAIMMSLDAPEAFQKLMDIIYATDYARTELACKSDGVDMVCQRGWYSSTDFWS
                     PDLFDQFIFPYIKGIADLAHKYGKKYGYVMTTGVEKLGPRLADAGVDLLYFVDPILDG
                     ISLEKAKELFGNRMTMVGGTNALSLQSGDPKRIENEVRRAIEILGPTKRFILHPMDAI
                     FPDTPWEGVEQMITAWKKYR"
     gene            complement(6220..7413)
                     /locus_tag="PLK08_01310"
     CDS             complement(6220..7413)
                     /locus_tag="PLK08_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52164.1"
                     /translation="MAKKNSINVALIGRGFMGRTHSNAWGQVNRFCNPPIKAVLHTAC
                     DNVADGAEDYCQTWGWQKFEQDFSKVCKDSKVDLVDLGTPNYIHASMAIEALQAGKHV
                     ACEKPLAGTLEDASKMVNAARKSKAKTFVWYSYRRCPAIAFAHQLVKAGKLGDIRHVR
                     AFYLQSWADESVPLAWRFDKKLAGSGAHGDLNAHIVDMTRFVTGQEITEISGAIAETF
                     IKKRNKMVGNVAGGIAAGLQKSQSMGNVTVDDTVLFLARFSGGAVASFEAARQATGNL
                     NRNGFEINGTKGSLMFNFERMAELQYFDATADEGLRGWTTINCTHGGSHPFVDMWWPD
                     AHAIGYEHTFTNMASDILRVLAGQKPIVPLADFEDAYQTQRVLEAVMLSATKKRPVKL
                     KEILN"
     gene            complement(7433..8470)
                     /locus_tag="PLK08_01315"
     CDS             complement(7433..8470)
                     /locus_tag="PLK08_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPS52165.1"
                     /translation="MDNKGYVFSFGPWNIHEGADPFGPTVRESIAFNKKLEQYKKLGF
                     DGVQFHDDDAVPNMNNLSKEQIIAEAAAVKKKLDEHGLKAEFVAPRLWEDPRTIDGGY
                     TSNCPECRKYAIDRSLRCIDIAKALGTDLIVFWLAREGTYIREAKDSCDAVKKIADAI
                     NAMFDYDKKIKIAIEAKPNEPMDHTYIPTIGHAIGLGLLTKDASRVGCVIESAHAILA
                     GLDPSDEMGYALAHDKLWTVHLNDQNGLKFDQDKTFGAVDLRRAFNQVRILENHGYAK
                     KGHMVGLDVKAMRTQKAEVATKHLSNSREIFLRLLEKVRTFDTKAEAELIKARDYEGL
                     EMLVINHLLGK"
     gene            complement(8499..9713)
                     /locus_tag="PLK08_01320"
     CDS             complement(8499..9713)
                     /locus_tag="PLK08_01320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPS52166.1"
                     /translation="MQWPNEFPGVYWLDDKEATAVLDVVKNGSLFRYYGLKAPKYVDK
                     YESLSKEYYGCKYTLGINSGTGALSTALSALQIGPGCEVIVPAFLWVATVGAVVQANA
                     IPVICEVDESFTMDPIDLEKKITPNTKLIIPIHMAGSPCNMEAIMKIARKHKIAVLED
                     CAQCNGGEFKGQKVGTFGDMGIFSLQLNKNMTCGEGGLIITNDEKLYYRAFAAHDMGL
                     IRVNGRLATPPEYALSWGQGRRMSELCGSVASEQIQKLPQITQKMRESKNRIKNLLKN
                     VSRLSFRKLTDVEGDSGPFMIIMLENAAKALSAVEKMKQAGLHNVFRLADYGLHVYSN
                     ILSLVNKVPLSPAGNPWKLCENQKSDYSYAKGACPKSDELFAKSILIPIPSCLSEQQE
                     AEAAEIIRQAMK"
     gene            complement(10607..10882)
                     /locus_tag="PLK08_01325"
     CDS             complement(10607..10882)
                     /locus_tag="PLK08_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52167.1"
                     /translation="MACLRKRSGTYYVQYYVGSTQKRVSLRTGNYQLANAAINQLEHR
                     LSQGHDSPLPTRTPIADAVQKYLDCMRAHKSPGSYQKDANIMRLIYT"
     gene            complement(11042..11113)
                     /locus_tag="PLK08_01330"
     tRNA            complement(11042..11113)
                     /locus_tag="PLK08_01330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11077..11079),aa:Arg,seq:acg)
BASE COUNT         2916 a         2680 c         2711 g         3167 t
ORIGIN      
        1 aaatacgaat ggactcctgg agacgatatc tgggacaaat attttcgcgg gaaatttgtg
       61 tctgaatatg agtttcctgt caaggttgaa aataacagac tggacatcga tatcagcatg
      121 ccgcagaatg gggataatga aaagaacaag ctgttatcgc gtcaggtatg tacagttaat
      181 ggaatgctta tttatcctgc ggcgaaagca gaagagttaa aggctacgct tgaatccatt
      241 aataaagagc gtaagcagca gtttgaaaaa caggcattat tgaatattgc gcctcaaaac
      301 ggaacaatgc cggccatttc aaaagagtac caggaatccg gatatgtgcc gtttgtacga
      361 aatttttgta aagacctgta ccataacagt cttccggtgg aagatgagat aggcaaaccg
      421 gttactattt cagccgcgat tggagaacgc gcgataagcc ggtttgcaat aagaccgctt
      481 tgtaatttaa agaatatcac gctggagatt tccgatctgg tttcggataa cggcatgaaa
      541 ttgccgccgg catgtgtttc atggcattgg ctaagatata tagagcagcc ggtggataaa
      601 tataaattta ggactctcga ttatcgtcct attgcagatt tgataatgcc ggctggctct
      661 tcggatgccg tgaaagatta taatcgtcaa tttctcgtgg aagtgactcc gtcgaaggct
      721 gtggcagccg gtgtctataa cggcacaatc accataaagc cggcaaatgc accagccgtc
      781 aagctggccc tgaaagtggt cgtctatccg tttaaattgg aaacgtatgc caacgacgat
      841 gaacgcattc agatatatga ccccaagtct ttcttatgcc actatggcaa cttgtggctg
      901 acaagtgacg acaaggaatt ttgggaatat gtcgataagg atttgtcgtt gatgaaaaaa
      961 tatcggatgg ctccaactgt gatgtttgac tggtttgcat ccatgaacga tttggacagg
     1021 tttatggaca tatacatgaa atacggattt catggatatc cgctttttgg cggctatgaa
     1081 cttttgcgga tatttgacgg taaaaaacct gaaaacactg gcatttcgcc cttcatcgaa
     1141 gttcttaaac aggtcaccag ccgtcagaag gaaaaacatt ggccgccgtt tttgttttat
     1201 tgcacagccg aaattcatac cggaatgcct gcgtatatca aagccaaagg atttctcaaa
     1261 caaatgaaaa atgctgttcc tgatgctaaa ctgctggtgc tgattaacaa aaatgatgag
     1321 atggacatgc ttgtaaattc tgatgcggac gttatcggcc ccaatgccgt atccatgacg
     1381 gaagacggta tagagaaagt ccggcaggcg aaaaagaagc tctggttcta cggctggggc
     1441 cgtgagagat tccgatgcgg cctgattgac tggcgaatac gaaacagggg cggcgtgagc
     1501 gagtggtatt cgtttgttgc gcaggctccg tttaatcctt tcgacggcaa gtatcccgat
     1561 tgctggaatg atgcaccgcc gtatttaggc ccgtcaggtc cgattccgac gatgaatctt
     1621 gaaattttca cggcagggcg catagacttt ctatatctgg ccacccttga gcagtggctg
     1681 aagcgggccg gcaaaataaa aagcccgcag agcattcagg catgcgcaaa agcccaaaaa
     1741 attatcgatg aattaaatga aactattgtg ccggattatt actattatta taagcgaaga
     1801 aaacttgcaa tgaatgcgat gtcgccattg gatataccga gaaagaaaat ttttggtttt
     1861 gccgatgagg attatgacaa acttcgccag agaattgcag agtcaatttg cgagttgaaa
     1921 gatgcctgta agtaaagctt tgatattatt gttgatgttt ctgccctgtg ccgccggcac
     1981 ggatgcgtgt aagaccgtaa aattgaccga tgatgaatat gacaatcatt cgccggtttg
     2041 gtcgccaaat ggcaaaaaga tagcatatgt ttccatacgc ggcagaaaga aatgcttggt
     2101 cttgcggccg gctgcttgca gtaaagacga aaaaatactg gctgaaaata ttgcggatac
     2161 cccagtgatt tcatggcagc cggatggcag attcattgca gttacagcat gcctcaagaa
     2221 tgaaaagctt gtctatgtta tcaatacggt aacgggccaa tgccgcgtat ttccaggtac
     2281 tttctgcaag tattctccta acgggaaaat attggctgta attcagaatg gcagaattgc
     2341 attgttaaat tctgttaccg gtaaagtaga caaaaaattt aaagtacccg ttaacgtaca
     2401 gggttttgat tggtcgccag acgggaaaat tattttttat tcctgcgacg gcgatctttg
     2461 gaaaatggat atagatggga gctccattgt ccgtctcttg aatcatacta aaggcagcaa
     2521 gggcgtgacg tcaccattta ttgaatcgcc taaggtatat ctcggcggga aaaaagtgat
     2581 tttcagtctg attgtcgacg gcctttatgc cggaactacg cataatatga caggcagtta
     2641 tgatattgca aatgcgtcat tcgcggtttt gggcgatgcc gacggatggg atgtttcgcc
     2701 atgcgggaga tggattgtta tggctgtcgg ttcaaacctg atgctttacg atttggataa
     2761 gaacaagact gtcatgattg gcaatggctc tgagccgtct ttttcgccgg atggccgccg
     2821 tattgcatat tcacattcaa aggaattatt cgcgcctggt gaaattttta taatggaaat
     2881 gccggaatcg tgttttaaaa aatgattttg gtggtttctc acgtcaatat aattttggca
     2941 gagtacatca agtcggcagg ggagtaaata ttcgtttttt tatactttca ggtattgtgc
     3001 accttatggc gtggtgatat ttttatgtat atgcaggaaa tagaattcac atctacggca
     3061 gacggttcgc atcagaaggc cttgttttat gttccaggtc cgaaagcgaa aaggcctttg
     3121 ccgcttgtgg tcggccttca ccagtggagc gttcatctgg acgaacgtcg caatgggccg
     3181 gcctattttt cgcagtgtcg caaacgtggt tgggcgttca tttatcctga atttcgcggc
     3241 cctaatgaca ggccgcaggc atgtgcgtcg ctgttggcgg ttcaggatgt aattgacgct
     3301 gtggattatg caaaaagtca tgccgccatt gatgaggata gaatatatat ggtcggagcc
     3361 agcggcggcg gaatgatgtc gttgataatg gctggccgat atcctgagat atgggcggcg
     3421 gtgagcgtat gggttccgat tttcgacctt aagaagtggt atgccgacgg catgaaacgt
     3481 ggcaatagat atccttccat gttggaagca tgttgtgggg gtgcgccagg cagttccagg
     3541 ggagttgacg ggcagtatcg cattcgttct ccgaaaagtt gcataaaaaa cgcggccaat
     3601 ctgtcgcttg acataaatgc cggtgttcac gatggtcata tcggggcagt tcccatatat
     3661 cattcgtttg atgcgttcaa tgttttggcg aaggccgccg ggtttgatga aaaaattgtt
     3721 ggtactgctg acataaaata catggtcact caggaagcaa ttccgccggc gttaagatat
     3781 aaaaaacggg aatctttcaa tcgcatgcac aaagttcttt ttcggcggca ggccgggaaa
     3841 gttcgtatta cgatttttga aggtggccat gaagttgacc cgacggcggc gtttgagttt
     3901 tttgtcggga agaaacgttc tagcagagaa tgatatcttg ctccgtatag cctgcacagg
     3961 ttctcatcag ttccgcaaga ctgaacgaac cgttgccaca atgggttagt atgacactgc
     4021 catcttccag cggttcgagc caatcggtat tgtgcgtagg ctggcctgac aacagtgtca
     4081 aaataagctg ccctattgca ccgttgacgt cgccttaaca ggcaagcggt attccatcat
     4141 ccgccaacaa tgaggcagcc atgcacacgc gatccatcag gtccggatag catccgacag
     4201 tcaatgcgtc aagctggaat tcttcgataa gttccttgat tgcaagataa actttcatcg
     4261 aatccaagcc gtcgttttcg cagacattgc atctgccgct tcgcgaaaca atgtttttcc
     4321 ataattgctc aacatcggta tttgaaattt ctttcgccct gttaagaatc cgtggtatat
     4381 ccagatttac aacccgaggc ctaattgttt tttcagtgcg aattcattaa ctgaaacttc
     4441 cgtcatgcca cgaacgtgat atccatccaa gcctatcttt gttcttcgga gcctgctctt
     4501 caaagccgta ccaagcagaa agtcctttgc tttctgctgc gattccacag attccagttg
     4561 gctataaata caaccatatg gaacttccaa atgctttaaa tatgcggtca gctgttgtac
     4621 tccgcacagt gccccagttt ccatgcccgg cagagaccaa agcagcagcg gcctgctgca
     4681 ctcttccaac aaatccagga caaggtaatc ctcgaaccag cttacagcta caagcacaat
     4741 ggcatcgaca cgtttttgtg aaactgatat tctagcggaa acagcttttt gaggcgtgtc
     4801 tattgatccc agattttcga cagtcattcc aagttcagcc aatatttcgg ctgcctgacc
     4861 tgccgcgtga gatgctctat ccgtaccaac ttcaagccga ctggacaatg caccaacagc
     4921 aatgctggga atcaatttgt tttttatgat agacatttat gtttcagcga tattttttcc
     4981 aggcggttat catctgctca acgccttccc acggggtatc cgggaaaatt gcatccatcg
     5041 ggtgaagtat gaaacgtttc gtaggcccga ggatttctat ggcacggcga acttcattct
     5101 caatacgttt ggggtcgcca gactgtaaag ataatgcatt cgttccgccc accatagtca
     5161 ttcgattgcc gaatagttct tttgcttttt ccaatgaaat accgtccaga atcggatcga
     5221 caaaatacaa cagatctact ccggcatcgg caagtcgagg cccgagtttt tcaacgcctg
     5281 tcgtcatgac atatccgtac tttttaccat atttatgggc caaatcggct atgcccttga
     5341 tgtatggaaa aataaattga tcgaaaaggt cgggtgacca gaagtccgtc gacgaatacc
     5401 agccacgctg gcaaaccata tcgacaccgt cacttttgca tgcaagttca gttctcgcat
     5461 aatcggtcgc gtaaataata tccataagct tctggaacgc ttctggggca tcaagtgaca
     5521 tcatgatggc gttttcggtt ccagtgaacc atacggcagc atccataccg aatgccgtcc
     5581 aggcctgagt gaaaaagcct ttttcatcgg caaatttctt gatctgcttc atgcggcctt
     5641 caaaccagac tttgtcacct tcgctcggtg gagcaaaaag atacttgatt ttatcaacat
     5701 cttctgggct gctcactgca tgttctacag ctctgggaat attataatcc tcgattatcg
     5761 gcacacagtt cggctgcaat ggccagcccg gcccttcatc gcctgttctt ctgactgcat
     5821 ggcgcaatgg gccagccgga gtctgatatg tccgcaccat tacaggatat ttatcatctc
     5881 caccgggttc acctatcggt attatgctat cctcaaaagt aacgtctttg gattggctcc
     5941 acggaaccga tatttcaaga tatggatcga gccctagcga tgcccacgcc tcacaacgtt
     6001 tgaattcatc ttcaagcgtc cacggcaggg gaaggttgtg cagatgctca agccgaagcg
     6061 tataccaata atcaaccggc ttgccgttgg tttgccattt caaatgctca ggggccggga
     6121 aaccgaatgt ccacgtggta agcggaatat gatccacagg ttcaccattg agtgcagcca
     6181 atattcgttc tttagacgtc attttatatg cttccaatct taatttagaa tttcttttaa
     6241 tttcacgggt cgtttctttg tggcggagag catcacagcc tccagtacac gctgggtctg
     6301 atacgcatct tcaaaatcgg ccagcggaac aattggtttt tgcccggcaa gtacgcggag
     6361 gatatcgctg gccatattcg taaaggtgtg ttcgtaaccg atagcgtgtg cgtccggcca
     6421 ccacatgtca acaaatggat gactgccgcc gtgcgtacag ttaatcgtag tccagcctcg
     6481 cagtccttca tcagcagtcg cgtcaaaata ctgaagttca gccatacgct caaagttgaa
     6541 catcagcgaa cctttcgtgc cgtttatctc aaatccgttg cgattgagat tgccagtagc
     6601 ctggcgagcg gcttcgaacg atgcaactgc cccaccagaa aacctcgcaa ggaacagcac
     6661 cgtatcatcg accgtcacat ttcccataga ctgtgatttc tgcaaaccgg ctgctattcc
     6721 accggcaaca ttgccgacca tcttgttgcg tttcttgatg aacgtttcgg ctatcgcgcc
     6781 ggatatttcc gtaatttcct ggcctgtgac aaatcgcgtc atatctacga tatgtgcgtt
     6841 caggtcacca tgtgctccgc tgccggccaa ctttttatcg aaccgccatg ccagcggtac
     6901 tgactcatcc gcccagctct gcagataaaa tgcacgcaca tgacggatat cgccaagttt
     6961 gccggctttg acaagctgat gtgcgaaagc tatcgccgga caacggcgat agctgtacca
     7021 gacaaaagtt ttagccttgg atttgcgtgc tgcattgacc atcttgctgg catcttcaag
     7081 cgtaccggcg agaggttttt cgcaagctac atgcttgcca gcctgcaacg cttcgattgc
     7141 catcgacgca tgaatgtaat tcggcgtgcc aaggtcaaca aggtcaacct tgctgtcttt
     7201 gcagaccttg ctgaagtcct gctcaaactt ctgccagccc cacgtttggc aataatcttc
     7261 ggccccgtcg gctacgttgt cacacgctgt atgcagcact gcctttatag gcggattgca
     7321 aaaacgatta acttgtcccc acgcattgga atgcgtgcgg cccataaatc cgcggcctat
     7381 aagtgcaaca ttgatggagt tttttttagc catttgacat gtcctcaaac aattactttc
     7441 caagcaggtg attaatcacc aacatttcca agccttcgta atcgcgggct ttgatgagtt
     7501 ccgcttcggc cttcgtgtca aacgtgcgaa ctttttcaag caggcgcaga aaaatctcgc
     7561 ggctgttgga caaatgtttc gtggccactt ccgctttctg ggtacgcatg gctttgacgt
     7621 cgaggcctac catgtggcct ttcttcgcat agccatgatt ttcaagtatc cgaacttgat
     7681 tgaacgcgcg acgaaggtcg acagcgccga acgttttgtc ctggtcaaac ttcaggccgt
     7741 tctggtcatt cagatgcacc gtccacaatt tgtcgtgagc cagcgcgtag cccatctcgt
     7801 ccgatgggtc caggccggca agtatcgcgt gcgcactttc aatcacgcag ccgacgcgcg
     7861 acgcatcttt cgtcagcaag cccagcccta tcgcgtgacc tatggtcgga atgtatgtgt
     7921 ggtccatcgg ctcgttgggt ttggcttcga tggcgatttt gattttcttg tcgtagtcga
     7981 acatggcgtt gattgcgtcg gcgattttct tcaccgcgtc gcaggagtct ttcgcttcgc
     8041 gaatgtaagt gccttcacgc gccaaccaga aaacgataag gtcagtgccg agtgcttttg
     8101 cgatgtcgat gcaacgcagc gaacggtcga tggcgtattt tctgcattca gggcagttgg
     8161 acgtgtagcc tccgtcgatg gtgcgcgggt cttcccatag acgcggggct acgaattcgg
     8221 ccttcaggcc atgctcgtcg agtttctttt taacggcagc cgcttcggcg ataatctgct
     8281 ctttggagag attattcatg ttcggcactg catcgtcgtc atgaaactgc acgccgtcga
     8341 acccgagttt tttatattgt tcgagctttt tattgaaggc tatcgattca cgcactgttg
     8401 gcccgaacgg gtctgcacct tcatgtatgt tccaaggtcc gaatgaaaat acgtaccctt
     8461 tattgtccat gatttttctc caatattata tgttaaattt atttcattgc ctgacggatt
     8521 atctcagccg cttccgcttc ctgttgttcg ctcaggcagg atggaatcgg aatcagtatg
     8581 gattttgcaa aaagctcatc acttttcggg catgcacctt ttgcgtatga ataatcgctt
     8641 ttttgatttt cacacaattt ccaagggttg ccggcaggcg aaagcggaac tttattaaca
     8701 agcgagagga tattgctgta gacgtgcaat ccatagtccg ccagcctgaa aacattgtgc
     8761 agacccgcct gtttcatttt ttcaactgcg gagagtgctt tcgcagcatt ctcaagcatt
     8821 attatcataa atggcccaga gtcaccttca acatcagtca attttctgaa tgaaagccta
     8881 ctaacatttt tcaaaagatt tttgatacga tttttggatt cacgcatttt ttgagtaatc
     8941 tgcggaagct tctgaatctg ttctgatgca acggaaccgc aaagctccga catacgtctg
     9001 ccctgtcccc acgacaaagc atattccggc ggtgtagcca atcggccgtt gacgcgtatc
     9061 aaacccatat catgcgctgc aaacgcgcga taatacagtt tttcatcatt ggtgataatt
     9121 agaccgccct cgccacaagt catgttcttg ttcaactgga gactgaagat gcccatatcg
     9181 ccgaacgtcc cgactttttg ccctttgaat tcgccgccgt tgcactgtgc acagtcctct
     9241 agaaccgcta ttttatgctt gcgggctatt ttcatgatgg cttccatatt acacggcgag
     9301 ccggccatgt gaatcggaat aatcagcttg gtgtttggcg taattttctt ttccaggtca
     9361 atcgggtcca tggtgaaact ttcgtcaact tcacagatga cagggatggc attggcctga
     9421 acaaccgccc caactgtggc aacccaaaga aacgccggaa cgattacctc gcaccccgga
     9481 cctatttgca acgccgacaa agccgttgac aatgcgccag ttccactgtt tatacccaac
     9541 gtatatttac agccgtaata ttcctttgat aaagattcat atttatccac atattttgga
     9601 gctttcaggc cgtaataacg gaataaggaa ccatttttga caacatccag tactgcggtc
     9661 gcttccttgt catccaacca gtaaactcct ggaaattcat ttggccactg catttttcac
     9721 cttgccttat acgttaattc ataaaacatg ttttatttat attttacaca tgtatagtat
     9781 aaggtactat tgttcttttg tcaactcggt aaatttcgac aagaaagatt tgattgcaat
     9841 aacacgcaac tttatgtaag aatttacgat atggatctaa aatataatca aaaaactcca
     9901 gatgctccgt caacgcagtg gcctgtcaat acgggaactg gtcaagcttt ccggcgtgac
     9961 ggctgggatg atttcctgta ttgaaaggaa caaaacatct cccagcctga ctaccctgca
    10021 aaagatatta tccgctttgg acacagacct tgcggaattc ttttcactca aaaaagaaac
    10081 cgacgatggc cctttctgtt gatgataact cgctgctttg gcgttgtccg cattgtcaca
    10141 ctggccacac tttcggacag gaacttttac cgataaccga cactccaaga aatggaggtg
    10201 ccgcgatgct gtaaaataat caaacaacaa aagacaattt gaaaaaccta cctcggagaa
    10261 tcagttttct gcaagtccat cagggtaggt tgttaatggt taatcttcca tactatgctt
    10321 ttgtagcaaa ttattgatac ggttaaagtc tacgatcccc gaagattcga caataggcat
    10381 aaagggagaa catatttgtt tcatgtcatt agatttgctt aattgctcta gctgttctcg
    10441 atctttcaaa aaatttataa attccttcac gcagctatat ttatgaaaga gagttggttt
    10501 gattccttca aggtacaaac cttcgatttt attaaacaat ttaagatctg ttagtggagt
    10561 atctaacatt attgattcga gataaataaa tctaacggag ttttttttaa gtatatataa
    10621 gcctcatgat gttggcgtcc ttctggtagc tgccgggcga tttgtgggca cgcatgcagt
    10681 cgaggtattt ctggacggcg tccgcaatcg gtgtgcgtgt gggtagcggc gaatcgtggc
    10741 cctgactgag cctgtgttcc agttggttta tggcggcgtt cgccagttgg tagttgcctg
    10801 tgcggagaga tacccgcttt tgcgtgctgc cgacgtaata ctgtacgtag tacgtaccgc
    10861 tgcgttttcg taaacatgcc atgttgagac tccttgcaaa aaagttgatt aacactcttt
    10921 gcgagtccca tttttcctga aattacaggg tacagtttag ggtacagttg accatgttta
    10981 ccgaaaaaga aaaggactca catttctgca agtcctttat tcttagatac ttctaaaaat
    11041 acgcacgaca ggattcgaac ctgtaacctt cggttccgta gaccgatgct ctatccaatt
    11101 gagctagcgg tgcatggctc ttatcttacc gaccgcaagc cgtttttcaa cgggcgcttg
    11161 cgacgagaag acgccttgcc gaggactgcc gcgaaagccc tgcttgcgac ccgccggggc
    11221 agcccacatc aagccgagat gagtaggata ccgcagatcg ccccgactgc caagagcgcg
    11281 gcggttcggg gcgaagtgtc gcacctcccg gcggtttggc tttcagtttg gttgcgactg
    11341 cgctggcttg aggtacttgt cgaaccagtc ggcgatgcgt tccgcgtcag gaccccactc
    11401 tccccagccg tggcccaatc ctggcttgat cacgatctgg aaaccaggat ccagcggacg
    11461 cagctcgaag tcgg
//
LOCUS       DAONFI010000021        11421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6364_length_11421_cov_4.512845, whole genome shotgun sequence.
ACCESSION   DAONFI010000021 DAONFI010000000
VERSION     DAONFI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6364_length_11421_cov_4.512845"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PLK08_01335"
     CDS             <1..1630
                     /locus_tag="PLK08_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52168.1"
                     /translation="DAYDLLDYHLQVPREYLDAGQITPLFHNVYSFYPLGAEMIYLFM
                     MSLQGGVNEGMYLATLVHGLFGVLAVAGMFFGLRDSLGRFRPWAAALMLATVPGVIFY
                     SWLAFSELAMICYMVLGLFWLKRWIVCRDIRTAIMTGLMCGCACSVKYLSVGMIALPI
                     FLVMFLFAIAASRRIWWHVITAVILCIVVMGPWLVRNAVFTKNPVFPLATKFFGRGHW
                     SSQVEQRWVHGHSGGRRPPVPAVPGWDAGSENRLKMLWTNFFGSEFSGLGVTWLAILS
                     SLWIVVEKKWRHDAWLFALMFIIAVQLAMWFLFAPNMPPRFILPSSAAAVLLAAEGLT
                     RLRWRGKSTAFLSSIILAAVVIFDFAGAFGIWLRQTKDTRDIVPVPADVFMRQMSPGA
                     DIYKLGPQAKVLLVGDAAVFYYPSNVIYCTPFDNNPLDIVPQEAVNKLANMGVTHILV
                     NWREIWRLAGTYGFPAKLTSGLYDLQLGGKTPPTLPVLDELIKRGATVQHFGGEYPFP
                     MPQERRKKWNPFLMPPNWPVFSIYTLPDFVTKNN"
     gene            complement(1800..3188)
                     /locus_tag="PLK08_01340"
     CDS             complement(1800..3188)
                     /locus_tag="PLK08_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPS52169.1"
                     /translation="MNIDRYFEISRRGSTITREIAGGVTTFMAMSYIIFVQVGILSLT
                     GMPAGGIIVATCLSAAIASVVMGLWANYPIALAPGMGENVFFTFTLCGVGAAAGAFRL
                     QWPEALGLVSISGVLFLLLSFVGFRTYVLNAIPQSLKAGIAAGIGLLISAVGLWYGNI
                     VKFNAIGGPGLESMTNNPCAWLAMCGILLVMILYAFRIHGAVMITLLCCGAGACLMGL
                     IPWPTKIFALPQGFDKTAGAFIGGFAGITRMLRGSFAMEVVMFTLVLLIMDMFDTIGT
                     LVGVAGQCGLMKNGKLEKASQALAADAVGTIIGASLGTSTVTSFVESAAGVSAGARTG
                     LAAIVAGACMAAAIFLSPIIQVIGGGIEVTPGAFKYPIIAPALVMVGAIMLKTIRDIN
                     WDDVTEYVPAFITVVIMPLTLSIATGIAAGFIAYAAGKLLTGKSRQCPAVVYIVAAIF
                     LARYVAGAFLNC"
     gene            3316..4326
                     /gene="hemB"
                     /locus_tag="PLK08_01345"
     CDS             3316..4326
                     /gene="hemB"
                     /locus_tag="PLK08_01345"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPS52170.1"
                     /translation="MSEHNSQSVGLRRNRRLRYSATLRDMLAEVHLQRDELIAPLFVR
                     SGSGVKSEISSMPGQYQFSADTALETVRRWADRGLRAVLLFGVTDDKNKDDVGSFAWA
                     DNAPVQTLVREIKRVLPEMLVVADTCLCEYTSHGHCGPLKEYRDGCFDVDNDRAIEYL
                     ARAAVSQSRAGADVVAPSAMMDNQVAAIRASLDDAAMPRTAIMSYSVKFASCMYGPFR
                     DAAESPPKSGDRKTYQMDFRSPRQCMLELQEDEEQGADIVMVKPAMTYLDILSQVRAN
                     TSLPLAAYHVSGEYAQIKFAAQAGAINEKAAVIEAVSAIKRAGADIIITYYAENLANW
                     LN"
     gene            4576..4905
                     /locus_tag="PLK08_01350"
     CDS             4576..4905
                     /locus_tag="PLK08_01350"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HPS52171.1"
                     /translation="MTLLAAPLTMLMKHQIGRRVKKLSELSVIWDELLPDIIREHTVL
                     DDFNNGTLKVVVDSPAVRFELQTILRSGIERELKTRFGGALNKIKIVPGQFYTLDRET
                     GQPRYSF"
     gene            5433..7160
                     /gene="ricT"
                     /locus_tag="PLK08_01355"
     CDS             5433..7160
                     /gene="ricT"
                     /locus_tag="PLK08_01355"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPS52172.1"
                     /translation="MTDSVDLPNNGQSAPFENDTQQTQPPAELRENSTESQPAPQTES
                     QPVASFAQNAFENSTEVSTDANSEEKQLPRISEAQTRESQIQPEESAAELAEEDFQLN
                     EADENLLDETYTESVQDFQENNSEGYELQNNVAPEGNTIVVRYGVMQFVGEFRHNLAV
                     VPTIGSKVVVRTERGVELGEVLMPVFRTEDGCGSCVRKITGEHLQKYLSKCGPEHPFR
                     RQGKVLRTANQQDIIDFRHLSLSADEAAKFCRENIARFKLNMKLVKVEHLLGGGRIIF
                     YFTAEQRVDFRELVKTLAGQFRTRIEMRQVGARDEARLVGDYERCGQRCCCQQFLKNL
                     KPVSMKMAKVQKATLDPSKISGRCGRLMCCLRYEDEGYEELRKRLPRRGIWVRLEDGA
                     IGKVVDTQILTQLVKIELPNREFEAVNVDEIAERDIPQPSEQELEDLARKPREKKEAA
                     PIVAPVTYFSAQTMMEVDAQLDVAATQSNQAENPPSEQAESERGEEKHGSRRRRHGRN
                     RKPGGDKNFQPSRDAQQQQKPENTGESQPHETRENRSKHKRRHHHYSKRPHDNNGQAN
                     QQQGSAGGE"
     gene            7163..10243
                     /locus_tag="PLK08_01360"
     CDS             7163..10243
                     /locus_tag="PLK08_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPS52173.1"
                     /translation="MGNGCFAGIDIGAVSVTAVVICPSGEQTNFDGWERTALPAGSVA
                     WFSPYRRTKGKPVVAAQTMLDEIRMAICGQTLRNVVLTGSGAKTAAGRIDAATVNEFS
                     AIAAGLSLLDIEARSVFEIGGETSKYLLLEREPASNEYSIIDYATNGDCAAGTGSFID
                     QQARRLRFEIESVGDICSRAARSAQIAGRCSVFAKSDMIHAQQKGYSPAEVLRGLCDA
                     VARNFRMAVVRSHRIELPVVFIGGVTANSAVVNSMKQVFELGDGLVVPNHAVHIPAAG
                     AAVTAMRAIGQIRDGQSSDPTEPEKFPTLDPLSLEKVILLRDCVKPFEHDGQGRIDAY
                     MGIDIGSVSTNVVVLDENGQVVDEIYTRTQGRPIEVVAGALREIETQWSAKLNIRGVG
                     TTGSGRELIGQLVGADTINDEITAHKTGAMFVGKTLLGGRVPDTIFEIGGQDSKFISL
                     SDGIVVDFTMNEACAAGTGSFLEERADELEVAIKGEFARLALSSKTPIRLGERCTVFM
                     ERDVNSYMQHGAKREDLLAGLCYSVVQNYINRVVRGRKIGSTIFFQGGTAYNDATAAA
                     FSMVTGKEIIVPPHNGVIGAIGSALLAQQKMADVQCGEPQTDYMRLMSEHGCVTASKF
                     RGYDLERVDFTLREFTCRACNNNCSIQEFTVAGEKTYWGDKCSDRYRKHVKTDRQAVI
                     DDVMCERNELLFDALHLPSPQEGAPKIGIPMTMFAWGQLPFWRTLLAELGFDVILGEQ
                     TNQRIIKAGLAAAVAEPCFPIIVAHGHMLNMAEKQVDYILAPSTLSVETRWTHTEAHL
                     CPWHQTLSLVTRRAPELKQFDMMRKYVTPLVEFQLGRAASTRQLERYFTKHHTAKMLG
                     ITAKKLRAAANKAFAAQDAFVAACHARGEAALETLRRTKQPGILLAGRYYNVHDAGMN
                     LAVARKLRDYYGVNVIPMDCVDLRDVDIRETNPNMYWALGREIIAAACFASQHDNLHI
                     IHITNFKCGPDSYVKQYIAQASGKPYLSLQFDGHSNDAGMITRCEAYLDSKGII"
     gene            complement(10250..10792)
                     /locus_tag="PLK08_01365"
     CDS             complement(10250..10792)
                     /locus_tag="PLK08_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HPS52174.1"
                     /translation="MLTVLVVLIVIDVVSLVVFFKLLSNYPVHHFDEVIPGVLYRSAQ
                     PDPERWKVLRDQYHIHTVVNFRYDDPGDEGMMFEKQFCKDNNIKLIRIALDDAPPTEK
                     ELTEFLNIVKNPANQPVLVHCELGRSRTGVMVAAYRIKCDGWTAAKAIEESKHFKQNI
                     RPVYVDYLNKIASQSANPDK"
     gene            complement(10891..>11421)
                     /locus_tag="PLK08_01370"
     CDS             complement(10891..>11421)
                     /locus_tag="PLK08_01370"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPS52175.1"
                     /translation="PQWEHEETTSFIIEMVGYLFLIFGLCIRVWAILYIGGKKSKELT
                     TTGPYSLCRHPLYLGTFSIVVGAGLCFENIPMLLATILIMVPIHLVVARMEDEHMAEL
                     FPQTYPEYKKLVRGYWPNFKNYRSSETIEIPIRSIRRVLVDTIAVLMIPQIEDLLELL
                     QENCHMPILWTYHMPF"
BASE COUNT         2809 a         2729 c         3140 g         2743 t
ORIGIN      
        1 ggacgcatac gacctgctgg actaccatct gcaagtgccg cgggaatatc ttgacgccgg
       61 acagattacg ccgctgtttc acaatgtcta cagcttttat ccgctcggcg cggagatgat
      121 ttatctgttc atgatgtcgc tacagggcgg tgtgaacgag gggatgtatc tggcgacgct
      181 ggtacatggg ctttttgggg tgttggctgt ggcgggaatg tttttcggct tgcgcgattc
      241 tttgggccga ttccggccgt gggctgcggc gctcatgttg gcaaccgtgc cgggggtgat
      301 attttattca tggctggcgt tttcggaact ggcgatgata tgctatatgg ttcttggcct
      361 gttctggctg aagcgatgga tagtgtgccg tgacattcgt acggcaataa tgacgggttt
      421 gatgtgcggt tgtgcatgtt cggtaaaata tctttcggtg ggcatgattg ccctgccgat
      481 atttttggtc atgttccttt ttgcgattgc ggcgtcgagg cggatttggt ggcacgtcat
      541 tactgccgtc attttatgca tcgtggtaat ggggccatgg ctggtgcgga atgcggtttt
      601 tacgaaaaat cctgtttttc cgctggcgac gaaatttttt ggcaggggac attggtcatc
      661 gcaggttgag cagcgatggg tgcatggcca tagtggcggc agacggccgc ctgtgccggc
      721 ggtgcctggc tgggatgcgg gcagcgagaa tcggttaaaa atgctgtgga ccaacttctt
      781 cggctcggaa ttttcaggac tcggtgtaac gtggctggcg attctttcat cgctgtggat
      841 agttgtggaa aaaaaatggc gacacgacgc atggctgttt gcgcttatgt tcataatcgc
      901 agtgcagctc gcgatgtggt tcttgttcgc cccgaatatg ccgccgcgtt ttattctgcc
      961 gtcgagtgcg gcagccgttc tattggctgc ggaaggattg acgcggctgc gatggcgagg
     1021 taaatcgacg gcctttttat catccataat attggcggcg gttgtgattt tcgattttgc
     1081 cggcgcattc gggatatggc tgcggcagac taaagatacc agggatatcg tacctgtgcc
     1141 ggctgatgtt ttcatgcgtc agatgtcgcc tggtgcggat atttacaaac tcggcccgca
     1201 ggcgaaagtg ctgcttgtcg gcgacgcagc cgttttttat tatccgtcaa acgtgatata
     1261 ttgcaccccg tttgataaca atccgctcga tattgttccg caggaagccg tcaacaaact
     1321 cgccaatatg ggtgtgacgc acattttggt gaattggcgt gaaatatggc ggcttgccgg
     1381 aacgtacggt tttccggcaa agctcacgag cggtctctat gatttacagc ttggcggaaa
     1441 aactccgccg actctgcctg tactggatga attgataaag cgcggcgcga cggtacagca
     1501 tttcggcggc gaatatccgt tccctatgcc gcaggaacga cgtaaaaaat ggaatccgtt
     1561 tctgatgccg ccgaattggc cggttttttc aatctatacc ctgccggatt ttgtcacgaa
     1621 aaataattag aacctatccc aaaaacatgg acgtcgccga aatcgagcaa ttttgcaggc
     1681 tggcaagaaa gaataatagc ggcatatttt ttaagttacc cgcaccaggt ttaattccac
     1741 cagctttagc tggtggaaat ttcacaaaat tttaaaaatt taaaaaagtt ttaaaaaatt
     1801 cagcaattca gaaaagcgcc tgcgacgtat ctggccagaa aaatcgcagc cacgatatat
     1861 acgactgcgg gacattgtct ggatttgccg gtgagcaatt tgccggcggc gtaggcgatg
     1921 aaacctgcag cgatgcctgt tgcgatactc agcgtcagcg gcatgataac gacggttatg
     1981 aacgctggta cgtattcggt tacgtcgtcc cagtttatgt ctcgtatggt tttgagcatt
     2041 attgcgccga ccattacgag tgccggcgcg attatcggat atttgaacgc acccggcgtt
     2101 acttctatgc cgccgccgat tacctgtatt atcgggctga ggaaaatcgc cgctgccatg
     2161 catgcgcctg cgacgatggc tgccaggccg gttcgtgctc cggctgatac gccggctgct
     2221 gattccacga acgacgtaac cgtacttgtg ccgagcgacg ctccgatgat agtgccgacg
     2281 gcatctgcgg cgagagcctg gctagccttt tccagtttgc cgtttttcat cagcccgcat
     2341 tggccggcta cgccgaccag tgtgccgatg gtgtcgaaca tgtccattat cagaagtacc
     2401 agcgtgaaca tgaccacttc catcgcgaaa ctgccgcgca gcattctggt tatgccggcg
     2461 aatccgccta tgaaagcgcc cgccgttttg tcaaaacctt gcggcagtgc aaatatttta
     2521 gtcggccatg gaatcagacc cataaggcat gcgcctgcgc cgcagcacag cagcgttatc
     2581 attactgcac cgtgtatgcg aaacgcataa agtatcatta ccagcaatat gccgcacatc
     2641 gccagccacg cgcatggatt gttcgtcatg ctttccagcc ccggcccgcc gatggcgttg
     2701 aatttgacga tattgccata ccagaggccg acggcagata tcaaaaggcc gatgcctgcg
     2761 gcgatgccgg ctttgaggct ctgaggtatg gcgttaagta catatgtgcg gaaacccacg
     2821 aaactgagca gcagaaacaa tacgccggaa atgcttacca ggccaagcgc ctcgggccat
     2881 tgcagtctga acgcgccggc tgccgcgccg acgccgcata aggtaaacgt gaaaaaaaca
     2941 ttttcaccca tgcctggcgc gagggcgatg ggataattgg cccaaaggcc catgactacc
     3001 gaagctattg ccgccgacag acacgtcgct acgattattc cgccggctgg catgcctgta
     3061 aggctgagta tgccaacctg tacgaagatg atgtaactca tggccatgaa tgttgtaaca
     3121 ccgcctgcaa tttcgcgggt tatggtgctg ccgcggcggc ttatttcgaa atacctgtcg
     3181 atgttcattt cagcctcgta ataaaagcaa taaaaatttt ttgaaaacca ctctaaatgg
     3241 ccgaaggcca taattatggg attttaacta ttattgcgat ttgccgcaag gttggatgtt
     3301 gtgtataatt ccggcatgtc cgaacataat tcacaatcgg ttggtttgcg aagaaatcgc
     3361 cggctgaggt atagcgctac tcttcgcgat atgctggcgg aagtgcattt acagcgtgat
     3421 gaactcatag cgccattatt cgtgcgcagc ggcagcggtg ttaaatctga aatcagttcc
     3481 atgcctggcc agtatcagtt ttcagccgac accgcgctgg agaccgttcg ccgttgggct
     3541 gacaggggcc tccgagcggt tctgctattc ggcgtaacgg atgacaagaa taaagacgat
     3601 gtcggcagtt ttgcgtgggc cgataatgct cccgtgcaaa cccttgtacg tgaaatcaag
     3661 cgtgtgctgc cggagatgct ggtggtggcg gatacgtgtt tgtgtgaata tacctcgcat
     3721 ggccattgcg ggccgctgaa agaatatcgc gacggctgtt ttgacgtaga taacgacagg
     3781 gcgatagaat atctcgcgcg ggccgcggtt tcccagtctc gcgccggtgc agacgttgtc
     3841 gcgccgtctg cgatgatgga taaccaggtt gcggcgatac gcgcatcgct cgatgacgcc
     3901 gccatgccgc gaacagccat aatgagttac agcgtaaaat ttgcgtcatg catgtatggg
     3961 ccgttccgtg acgcagccga gagtcctccg aaatccggtg accgcaaaac ataccagatg
     4021 gattttcgtt cgccgcggca gtgcatgctg gaacttcagg aagacgaaga gcagggcgcg
     4081 gatattgtca tggtcaaacc ggcaatgacg tatctggata ttttgtcgca ggtgcgtgca
     4141 aatacgtctc tgccgttggc ggcgtatcac gtaagcggcg aatatgccca gattaagttt
     4201 gccgcccagg caggcgcgat taacgaaaag gctgccgtca tagaagccgt ttccgccatc
     4261 aaacgcgcag gcgccgatat catcataacc tattatgcgg aaaatctggc taactggctg
     4321 aattgaagaa gcgtcgtcac tgtttattcc atatgcaaat ctgaaatgaa ataagataaa
     4381 tttccttcaa aataatgccg atatatattt aagtggcgaa tatatgttga ggaacggttc
     4441 gacttgtttg tcggctgtac aaatctcttg atatagccgc ctgacggaag tatgatacct
     4501 gctgttttga tggagaccac tgtgcaggat gcacaattaa gaacaatctg gcagcaaagg
     4561 cagccgaagg agacggtgac acttcttgcc gcgccgctga cgatgttgat gaagcatcaa
     4621 atcggcagac gcgtcaaaaa gctttccgag ctttccgtta tatgggatga gcttttgccc
     4681 gacattatcc gcgaacacac cgtgctggac gatttcaaca acggcacgct gaaggtggtg
     4741 gtggactcgc cagcagtgcg ttttgaactt caaaccatcc ttagaagcgg aatagaacgt
     4801 gaacttaaaa cccgttttgg cggtgctctg aataaaatca aaatcgttcc aggccagttt
     4861 tacacgttgg acagggaaac cggccaacct cgctactctt tctaattttt ttatctcgaa
     4921 agctggtaat ccagtgaatt ccgcaacctg tctggggaaa cgtaatcctt acagccatat
     4981 ttaaagggat tgagtattat atataaaaaa tgtatactgt ttccttgcaa gtgttgttgt
     5041 aatttatgcg gcttgccagg aaagatggaa ttttatcatg aaatgtcttt ttgaggcaaa
     5101 tgcgccgcaa aggctggcca tgatgaaact gagggtttga acaacattaa tatggccttt
     5161 gtaatttttt tacggttttt aaaagatgtc tgcccaagaa cattttttgg gaaagctgct
     5221 ataaaccgta tataaaaata tccgctggca ggcgaaataa gtagactgaa cgtgataaaa
     5281 tgctgcggat tttttgggaa attacgataa aaaagcccgg ttgcttgcgt atggacctcc
     5341 ggtgacgggc cggggacgtt aaaaacaaaa aatctctgtg ggccgccgca gattatctaa
     5401 cattcgtctg tacggatgtc ggagtaaatg acatgactga ttcggtagat ttgccgaata
     5461 acggccaatc cgcgcctttt gaaaacgata cgcagcaaac gcaaccgcca gccgaactgc
     5521 gggaaaattc gacggaaagt cagcctgccc ctcaaactga aagtcagccg gttgcgtctt
     5581 ttgcgcagaa cgcttttgaa aattcgactg aagtttcgac tgacgcgaat tctgaagaaa
     5641 aacagctgcc gcggatttca gaggctcaaa cgcgtgaatc tcaaatacag cccgaagaat
     5701 ccgccgccga actcgccgaa gaggattttc agctcaatga ggctgatgaa aaccttttgg
     5761 atgagaccta tactgaaagc gttcaggatt ttcaggaaaa taattccgag ggctatgagc
     5821 tgcagaataa tgttgcgccg gaaggtaaca ccattgtggt gcggtacggc gtgatgcaat
     5881 ttgttggcga gtttcgtcac aatttagccg tagtgccgac gataggttca aaggtggtcg
     5941 ttcgtactga gcgtggcgtt gagcttggcg aagttctcat gccggttttt cgtacggagg
     6001 atggctgcgg cagttgcgta cgcaagataa ccggcgaaca tttgcagaaa tatctatcga
     6061 aatgcgggcc tgagcatccg ttccggcggc agggcaaggt attgcgtaca gccaaccagc
     6121 aggacatcat cgattttcgc cacctgtcgc tttctgccga cgaagccgcc aaattctgcc
     6181 gcgaaaatat cgctagattc aaactgaaca tgaaactcgt caaggttgaa cacctccttg
     6241 gcggcggcag gataatattc tatttcactg ccgagcagcg tgtggatttt cgcgaacttg
     6301 taaaaacgct ggctggccaa tttcgtaccc gtatagaaat gcgtcaggtt ggtgcgaggg
     6361 acgaggcgcg gctggttggg gattatgaac gttgcggcca gcggtgctgt tgtcagcagt
     6421 ttttgaaaaa tctcaagccg gtgtccatga agatggccaa ggtgcagaag gctacgctcg
     6481 acccgtcgaa aatttcaggc cgttgcggca gattgatgtg ttgcctgaga tatgaagacg
     6541 aaggctacga ggagcttcgc aaacgtctgc cgcggcgcgg catatgggta cggctggaag
     6601 atggcgcaat cggcaaggtc gtcgatacgc agatattaac gcaacttgtg aagatagaac
     6661 tgcccaatcg cgagttcgaa gccgtcaatg tcgacgaaat cgccgagcga gacattcccc
     6721 aaccgtccga acaggaactt gaagacctcg cccgcaagcc gcgcgaaaag aaagaagcag
     6781 cgccgatagt ggctccggtg acttatttta gtgcgcaaac catgatggaa gtagacgcgc
     6841 agcttgatgt cgccgcaacg cagtcaaatc aggctgaaaa cccaccctca gaacaggcgg
     6901 aatctgaacg tggcgaagaa aaacacggca gccgtcggcg aagacacggc agaaatagaa
     6961 aacctggcgg cgataaaaat tttcagccca gccgtgatgc gcagcaacag cagaagcctg
     7021 aaaacaccgg agaatcccag ccgcatgaaa cgcgggaaaa tcgcagtaaa cataagcgac
     7081 gtcatcatca ttacagtaaa agaccgcatg ataataacgg ccaggcgaat cagcaacagg
     7141 gaagcgccgg aggtgaatga aaatgggcaa cggttgtttc gcggggattg acatcggcgc
     7201 cgtcagtgtg actgctgttg tcatttgccc gtcgggggag cagacaaatt ttgacggctg
     7261 ggaaagaaca gccctgcctg ccggcagcgt tgcatggttc agcccatatc gcaggacgaa
     7321 gggtaagcct gtcgtcgctg cgcagacgat gctcgacgaa atccgtatgg ccatttgtgg
     7381 ccaaacgttg cgtaacgtcg tattgaccgg cagtggtgcg aagacagccg ccggccgaat
     7441 tgacgccgcg accgtgaatg aattttccgc gattgccgca ggcctctcac tgctcgatat
     7501 agaggctaga agcgtttttg agataggcgg tgaaacaagc aaatatctgc tgctggaaag
     7561 agagcctgcg tctaatgaat attcaataat cgattatgcc accaacggcg actgtgctgc
     7621 cggtacgggc agtttcatag accagcaggc ccgccgcctg cgttttgaaa tcgaatccgt
     7681 cggcgatatc tgcagtcgag cggcccgctc ggcgcagatt gcagggcgat gcagcgtttt
     7741 tgccaagagc gacatgattc acgcccagca gaaaggttac tcgccggcgg aagtgttgcg
     7801 gggtctgtgc gatgccgtcg ccaggaattt ccgcatggct gtcgttcgca gtcatcgcat
     7861 agaattgccg gtggttttta tcggcggagt gacggcgaat tctgcggtgg tcaattccat
     7921 gaagcaggtt ttcgaactcg gcgatggcct tgtcgtgccg aatcatgcag tgcatatccc
     7981 tgccgccggc gcagccgtca ctgccatgcg tgctatcggg cagatacgcg acggccaatc
     8041 gtcagaccca actgagccgg aaaaattccc gacgctcgac ccgcttagcc ttgaaaaagt
     8101 tattctgctg agagattgcg tcaaaccgtt cgaacatgac ggccagggcc gtatcgacgc
     8161 gtatatggga atagatatcg gcagtgtatc gacgaacgtc gtggtgctgg acgaaaacgg
     8221 gcaagtggtg gatgaaattt atacccgtac gcagggccgg cccatagagg tagttgccgg
     8281 cgcgcttcgc gaaatagaga ctcagtggtc ggcaaagctc aacatacgtg gcgtcggcac
     8341 gacgggcagc ggacgcgaac ttatcggcca gcttgttggc gccgacacga taaacgatga
     8401 aataaccgca cataagacgg gagccatgtt cgtcggcaaa acgctgcttg gcggacgggt
     8461 gccggatacg atattcgaaa taggcggcca ggacagtaaa ttcatatcgc tcagcgacgg
     8521 catagtggtg gatttcacca tgaacgaagc gtgtgcagcc ggcacaggca gctttcttga
     8581 agaacgtgca gacgaacttg aagtggctat caaaggtgaa tttgcgcggc ttgcactttc
     8641 gagcaaaacg cctattcgtt taggcgaacg ctgtaccgta ttcatggaac gcgacgttaa
     8701 cagttatatg cagcacgggg cgaaacgtga agatttgctg gcagggttgt gttactcggt
     8761 cgtgcagaac tacatcaacc gcgtagtgcg aggccgtaaa atcggcagta cgattttctt
     8821 tcagggcggc acggcttaca acgatgcgac agccgccgcg ttcagcatgg tgaccggcaa
     8881 ggaaatcata gtgccgccgc ataatggcgt tatcggggcg ataggttctg cactattggc
     8941 tcagcagaaa atggcggacg tacagtgtgg tgagccgcag accgattata tgcggctcat
     9001 gagcgagcac ggctgcgtga ccgcgtcaaa gttcagagga tacgatttgg aacgggttga
     9061 ttttacgctg cgggagttta cctgccgcgc ctgcaacaat aactgttcca tacaggaatt
     9121 cactgtcgcc ggtgaaaaaa cgtattgggg cgacaaatgc tcggaccgtt atcgcaaaca
     9181 tgtcaagacc gacaggcagg ctgtcataga tgacgtcatg tgtgaacgta acgagctgct
     9241 tttcgatgcg ttgcatctgc cgtcgcctca ggaaggcgcg ccgaaaatcg gcatacccat
     9301 gacaatgttt gcttggggcc agctgccatt ctggcggacg ctgcttgcgg aactgggttt
     9361 tgacgtaatt ctcggcgaac agaccaatca gcggataatc aaagcaggcc ttgctgccgc
     9421 cgtcgccgaa ccgtgttttc cgataatcgt agcgcacggc catatgctta atatggcaga
     9481 aaaacaggtc gattatattc tggcccccag cacgctttct gttgaaacca gatggacgca
     9541 taccgaagca cacctttgtc cgtggcatca gacgttgtcg ctggtgacgc gccgcgctcc
     9601 ggaactcaag cagttcgaca tgatgcggaa atacgttact ccgctcgtgg aatttcagct
     9661 tggccgcgcc gcatccacgc gacaactgga acggtatttc acaaaacatc acactgcaaa
     9721 aatgctgggt attaccgcta aaaaacttcg agctgccgcg aataaggcat ttgccgccca
     9781 ggatgctttc gtcgccgcat gtcacgccag aggtgaagcg gcccttgaaa cgctacgcag
     9841 gacgaaacag cccggcatat tgctggctgg gagatattac aacgtccatg atgccggaat
     9901 gaatctcgcc gtcgcaagaa aattgcgtga ttattacggc gtaaacgtaa ttccgatgga
     9961 ctgcgtggat ttgcgagacg tggacattcg ggaaacaaat ccgaacatgt actgggctct
    10021 gggccgggaa atcattgctg ccgcctgttt tgcatcgcaa cacgacaacc tgcacataat
    10081 ccatatcacc aacttcaaat gtggccctga ttcatatgtg aaacaataca tcgctcaggc
    10141 atccggcaag ccgtatcttt cgctgcaatt cgacggacat tcaaatgacg ccggcatgat
    10201 aacccgctgc gaagcgtacc tggattccaa ggggattatt tgaacagggt tatttgtcgg
    10261 gattcgcaga ttgagatgcg attttgttca gataatcgac gtaaaccggc cgaatgttct
    10321 gcttaaaatg tttcgattct tcgatagctt tcgccgccgt ccatccgtca cattttattc
    10381 tgtatgccgc caccattaca cccgtgcggg aacggccgag ttcgcaatgt accagtaccg
    10441 gctggttagc cggattttta acgatattca gaaattcggt gagttctttt tctgtcggcg
    10501 gggcgtcatc gagggcgatg cgtatgagtt tgatgttgtt gtctttgcaa aactgttttt
    10561 cgaacatcat gccttcgtca ccagggtcgt cataacggaa atttacgact gtatgaatgt
    10621 ggtattggtc gcgcagaact ttccatcgct cagggtcggg ctgtgcgctg cgatacagaa
    10681 cgccaggtat gacctcgtca aagtggtgca ccggataatt agaaagcagt ttgaaaaaaa
    10741 ccaccagcga gacaacgtct atcactatca gtacgacaag taccgttagc acagatttat
    10801 ttctgagcat gtttctgagg ctagttttat ttacgaaaac catatatgaa tatttttgtg
    10861 gtaaaccaaa agttaaaaac tgcggttcat tcagaatggc atgtgataag tccagagaat
    10921 gggcatatga cagttttcct gcaacagttc cagcaaatct tcgatttgcg gaatcatcag
    10981 aacggctatc gtgtcgacaa gtacgcgccg tatcgagcgt attggaattt ctatcgtttc
    11041 cgaagagcgg taatttttga aattgggcca gtaaccccgc acgagttttt tatattccgg
    11101 atatgtttgc gggaaaagct ccgccatgtg ttcgtcttcc atgcgggcta ctacgagatg
    11161 tatcggcacc attataagta tcgtcgccag gagcataggg atgttttcaa aacatagtcc
    11221 tgcgcctacg actatgctga acgtgcccag atacaacgga tgcctgcata gcgaataagg
    11281 ccctgttgtt gtgagttctt tggatttttt tccgccgatg taaagaatcg cccatacgcg
    11341 gatgcatagg ccgaatatga ggaatagata tccgaccatt tcgataataa aagatgttgt
    11401 ttcttcgtgt tcccattgcg g
//
LOCUS       DAONFI010000022        11157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6592_length_11157_cov_5.101333, whole genome shotgun sequence.
ACCESSION   DAONFI010000022 DAONFI010000000
VERSION     DAONFI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11157
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6592_length_11157_cov_5.101333"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1444
                     /locus_tag="PLK08_01375"
     CDS             233..1444
                     /locus_tag="PLK08_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPS52176.1"
                     /translation="MATKTINDIKIGGRTVLMRVDFNVPLKDGVITNDNRIVEALPSI
                     RKVIADGGKLVLMSHLGRPDGEVKPEYSLKPAVDRLSQLIGKPVKLGPSWPIAGDEAK
                     AAIAAMNPGDVLCLENVRFDSREDTKDLDQVKQFGAELAAMGDLYVNDAFGTCHRKHA
                     SMWGCPLAVQAKGGAAVAGYLVEKEIKYLSDAVSNPVRPFLAILGGAKVSDKIKLIST
                     LLTKVDKIIIGGAMAYTLLKAKGVNVGISRVEEDQIDAMKELLAKAGEKIVLPVDHLT
                     AAAFDSDAANVTAGVEIPADQMGLDIGPKTIELFSKIVLGSKTIVWNGPMGVFERDAY
                     AAGTKAIAEAMAKATDAGAVTVIGGGDSATAVAKFKLQDRMTHVSTGGGASLTYLEGK
                     PMPPLEVLDQK"
     gene            complement(1681..3009)
                     /locus_tag="PLK08_01380"
     CDS             complement(1681..3009)
                     /locus_tag="PLK08_01380"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPS52177.1"
                     /translation="MDACRKGINVAIVGCGTVGGATARLLTTDRDILVNRLGCDVNLK
                     YIVDLNLKHAKELGLPEKLFQTDFNVALSDPEIDVVVELVGGLNFARELIEKSLIAKK
                     HVVTANKALLAHHGTELFSLARNNGVSISFEASCGGGIPIIQPLCCSLLANRIDAIYG
                     IVNGTCNYLLTEMSQQGRSYADALREAQADGLAEADPTLDVAGIDTAHKLTILSSLAF
                     GQRIDFDAIPCQGIDKLEICDIKWGSELGYVIKLLAIALRQGDGLCLCVRPAFISIKH
                     PLAWVAGPFNAISVYGHAVGHTMFYGRGAGGLPTASSVAADIANIALGNAQSQFEKLR
                     IWSDVAKPAKQLPVNEIKSRYYIRVMAEDKPGVLAKIADIFGRQNISIDAVLQRDYNR
                     PDATRPVIITTHTACEGNVRTAIEEVDALDSIKAKPVCIGIVDEYPEFVK"
     gene            complement(3147..4259)
                     /locus_tag="PLK08_01385"
     CDS             complement(3147..4259)
                     /locus_tag="PLK08_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPS52178.1"
                     /translation="MKLFFIRMMIAEIISDSFFEAQLDADRIWLLWLMVLSSVLLLVY
                     GADKLVAAAVQIARSLGFSPVIIGATIVSLGTTAPETFVSVTAAFSNNPGLALGNGVG
                     SIICDTALIFGLACCLCRLPKNRFLLVRQGWLQLAAGTLLTASFLVLAWMKGSFYGVV
                     MPRWMGFSFVVLLIGYMGISAHWARLHPEIIPEEAMDGQNNGRPTRKSKFGAVAVMLV
                     TMAMGLLLVLLGSDIMVGSTARICIHYGVPESVLAVTLVAFGTSLPELVTAIVSIIKG
                     HPELLVGNIIGADILNVLFVIGASAAATPLLVPTEFFYLHLPVMMAALILLRLFIFAK
                     GDRFHRWYGIPFLSLFAGYLAILFVMISTGHLKLHG"
     gene            4565..5839
                     /locus_tag="PLK08_01390"
     CDS             4565..5839
                     /locus_tag="PLK08_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPS52179.1"
                     /translation="MSLKIRCSECHKKISVDEAFAGSMCRCPYCKSIVLVPKQISRAA
                     DSVGRPGSPAIRPTIPTVGRPTAPGQTTISAPGHETMPSPVLREMKQPQPAEAGIPGG
                     KSSYIASMDSKAVENKKPAQEEKAVQKTSTKKPAATSATVEEAVVDAVKVDTSKLSKE
                     QIAAIPTANPIMFQGIVSLALIVVLLAMIGACIFLGYRMFASKERQNSDSIYSSQEIA
                     KEIQATSYATEDVNPFEYNTGASLICNQFKITGPVVFCIDAGTSMGDKYCLARDMVRA
                     SVLSMKSSENFAVIVNQDGGTNVITPMTAGGPEGEAKIRQKLMEQADDGTVVLGGAPM
                     LEEAILSASEFKPKTIVLLIADKDIDSPKRLIADLQKSGADLVIISLDAKPGQDTMIK
                     ELAKNAGQNTQLKLYNVEELEKSYEKCNLPAD"
     gene            6017..6742
                     /locus_tag="PLK08_01395"
     CDS             6017..6742
                     /locus_tag="PLK08_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPS52180.1"
                     /translation="MAVTRKRIEGNLAAIRSRIAAAAARSGRDAADVKIVAVTKSVDT
                     ETIRNLIDAGITALAENRVQQFVTRAEEINRYLQRRRNPLASPVEWHLIGHLQRNKVK
                     SAVEISAMIHSVDSLRLAEEINERCEKMNRRLPVLLQVNCSQEEQKYGCAVGAAIHLG
                     ELMAQMKHIELSGLMTMGPMDGNPDGTRHAFVRLRELFEEMRHENIGGRDFRHLSMGM
                     SGDYEIAVEQGATMVRIGSALFE"
     gene            complement(6725..8632)
                     /locus_tag="PLK08_01400"
     CDS             complement(6725..8632)
                     /locus_tag="PLK08_01400"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPS52181.1"
                     /translation="MSREWLLANRLGSYSSSTTAGCNTRRYHALLTAATNPPVGRIVA
                     LSMLMEQVYTSQGKFELACNEFPNGVFAPQGWRDLRTFTKGIIAEYDYRIGDRQTLKK
                     RVLLCRDSNTLIVRYELSGEAEKIIIRPFTPMRDFHHLANAGYHPDFEVSNGRLAVTA
                     GEQPKLVISCDRAEFLPDMQWWFNFYYRTDAARGQDFMEDLPSAGYFTWRPSKKKESC
                     EIVASLDGNVAVKFLDEYNKLLEESEEIDAGVSGCPDMMRCLAQSGNVFLVDRKTPDD
                     FDGASILAGFPWFADWGRDTFIALPGLLLSTGRFELARRVFKTYADNISQGMIPNRFD
                     DYGLAPHYNSIDASLWFTLAALRYIRADDSHEGRCFEREVLLPACKTIVDQYRAGTMF
                     GIHEDTDGLLVGGDENTQLTWMDVRIDNESVTPRYGKAVELNALWYQANALLGDIDAK
                     YAVAARKTAGAFINNFWLENAGYLADCVYPYGPDKSLRPNQILAAALPYSPLKPAQIK
                     SIVKVVREKLLTPLGLRTLSPDDWRYKGRYEGPQYIRDRAYHQGTAWAWLMGFYIEAS
                     IKTGASRAQLARLMKPFEKHMAEGCLGQINEIFDGDAPHTPRGCFAQAWSVAEVLRAT
                     EMIARLSLKKG"
     gene            complement(9306..9911)
                     /gene="lexA"
                     /locus_tag="PLK08_01405"
     CDS             complement(9306..9911)
                     /gene="lexA"
                     /locus_tag="PLK08_01405"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPS52182.1"
                     /translation="MQTVTPRQLEILRYIRDFRRSHGYSPTMQEIGDHLSLTKVTVFE
                     HVGALEKKGVLLRGEKHRARSLVVSDDFVFPDEQEESLPFVGRIVAGVPLEAFENCER
                     MNLNEEFPADGSTFVLEVAGESMINDHIMDGDYVICQRRNTARNGEIVVALLNDGETT
                     LKRIYREKGKIRLQPANDAFAPIVVDKCEIQGVVIGLMRKY"
BASE COUNT         2918 a         2919 c         2661 g         2659 t
ORIGIN      
        1 aaagaaaaaa ttacttttcg acaaaacttt aaacatggct gattcgtcgc ctgcggcggc
       61 aagcagacgg ctgaaatttc ctaaaagagg aaagaaaatg gttgaaaaac tttcatcagc
      121 cgcttgaaat tttcagcgat atttgcagaa tagcttttcc ggtgcggtta tgcagtcagg
      181 caggccgctt atggcaaaat ccttaaacaa tcaattattt atgaggtata tcatggccac
      241 aaagacaatt aacgacataa aaatcggcgg cagaaccgtt ctgatgcgcg tcgatttcaa
      301 cgtaccgctc aaggacggcg taattacgaa cgacaatcgt atcgtagaag cattgcccag
      361 catacggaaa gtcattgccg acggcggcaa actggtgctt atgagccatc tcggccggcc
      421 ggatggagag gtcaaacccg aatactccct gaaacctgcc gtcgaccgtc tgtcgcagct
      481 tatcggcaag ccggtaaaac ttgggcctag ctggcccatc gccggcgacg aagcaaaagc
      541 cgccatcgcc gcaatgaacc cgggtgacgt gctctgcctt gaaaacgtcc gattcgacag
      601 ccgcgaagat accaaagatc tcgaccaggt aaaacagttc ggcgcggaac tggcggctat
      661 gggcgatttg tacgtcaacg atgcgttcgg cacatgtcac cgcaagcatg cgtccatgtg
      721 gggttgcccg ctggccgtcc aggctaaggg cggtgcggcc gtagccggct atctcgtgga
      781 aaaggaaata aaatatctaa gcgacgcagt atcgaatccg gttcgcccgt ttctggcaat
      841 actgggcggc gcaaaggttt cggacaaaat caaactcatc tccacactgc tgacgaaagt
      901 cgataaaatc atcatcggcg gggcgatggc ttatacgctg ctcaaggcaa agggcgtgaa
      961 cgtcggcatc agccgcgtcg aagaagacca gatagacgca atgaaggaac ttctcgccaa
     1021 ggccggcgaa aagatagttc tgcccgtcga ccacctcact gcagccgcgt ttgattccga
     1081 cgcggctaac gtcacagccg gcgttgaaat accggcagac cagatgggcc tcgacatagg
     1141 ccccaaaacc atagaacttt tcagcaaaat tgtgctcggc agcaagacaa tcgtctggaa
     1201 cgggcctatg ggcgtctttg aacgcgacgc atacgccgcc ggcacaaaag caatcgccga
     1261 agcgatggcc aaggctaccg acgccggcgc agtcacggta ataggcggcg gcgacagcgc
     1321 aacggcggtg gctaaattca aactccagga caggatgacc cacgtctcca caggcggcgg
     1381 agccagcctt acatacctgg aaggcaagcc gatgcctcct cttgaagtgc tggaccagaa
     1441 atgacttacg gctgactgcc tattcttgaa aagttctata atttataaag ctttatgaag
     1501 gccattctca ggagttccag ggaatggcct gtgttttgtc ttattacagt taccgtcagg
     1561 atatacagta actaaggccg aaatcctgag tctcatcttt gcggataatg cctgaatatt
     1621 tacggtcttt ttcaagcatc aacagaacga ttctcaaaat gaactttttt gcagacagtt
     1681 ctatttgacg aattcgggat attcgtcaac tattccaatg cagaccggct tggcctttat
     1741 cgaatccagt gcgtcgactt cttcaatcgc cgttcgtaca ttgccttcgc aagccgtgtg
     1801 tgtggttatg atgaccggcc gcgtggcgtc cggcctgtta taatcccgct ggaggactgc
     1861 gtctatactg atattctgtc ggccgaaaat atcggcgatt ttcgcaagta cgccgggctt
     1921 gtcttcagcc atgacgcgga tgtagtagcg gcttttgatt tcgttgaccg gaagctgttt
     1981 ggccggcttg gcgacgtcgg accagattct aagtttttca aactgcgact gtgcattgcc
     2041 cagtgcgata ttcgcaatat ccgccgccac cgatgacgca gtcggcaatc cgcccgcgcc
     2101 tctgccgtaa aacattgtgt ggccgacggc atggccgtaa acgcttatgg cgttaaacgg
     2161 gccagccacc caggcaagcg ggtgttttat ggaaataaac gccggccgaa cgcaaaggca
     2221 taatccgtcg ccctgacgca acgctatagc cagcagtttt atgacatatc caagttcact
     2281 gccccacttg atgtcgcata tttcgagttt atctataccc tgacacggga tggcgtcgaa
     2341 gtctatcctc tggccgaacg ccagcgaaga aagtatcgtc aatttatggg ctgtatctat
     2401 gccggcgaca tccagagtcg ggtccgcctc cgccagcccg tcagcctgcg cctctctgag
     2461 tgcgtcggcg taacttctgc cctgctgtga catctcggtc agcagataat tgcatgtgcc
     2521 gttgactatg ccgtaaatgg cgtcaatcct gttagccagc aggctgcagc acaacggctg
     2581 aattatcgga atgccgccgc cgcaactggc ttcgaacgaa atgcttacgc cattgtttct
     2641 tgccagcgaa aaaagctcag tgccatgatg tgcaagcagc gccttgttgg cggtaaccac
     2701 gtgctttttg gctattaacg atttttcaat gagttccctg gcaaaattca atccgcccac
     2761 cagttccaca acaacgtcta tttcagggtc gctaagagca acattgaaat cggtttggaa
     2821 cagtttttcc ggcaggccca attctttggc gtgtttgaga ttcaaatcga cgatatattt
     2881 caggttgacg tcgcagccga ggcgattgac gaggatatct ctgtcagtcg ttaagagacg
     2941 ggccgtggct ccgcctacag tcccgcagcc tacaatcgca acatttatgc cttttctgca
     3001 tgcatccatt gaatttctcc tgcaaagccg gatatcatca tttctgagcg ggcaaaaacc
     3061 cttaaaataa agatgatacg cacttgagat taaaaatcaa actacttttg tggcagatta
     3121 gagcaagttt aacatgctct aatccgtcat ccgtgcaatt tgagatggcc agtcgatatc
     3181 attacgaaaa ggattgccag atatcccgca aacagcgaaa gaaatggtat gccgtaccag
     3241 cggtgaaaac ggtctccctt cgcaaaaata aacagccgca gcagaatcag tgctgccatc
     3301 atcaccggca gatgcagata gaagaactcc gtgggcacaa gcagcggcgt agcggcggca
     3361 gaagcgccta tgacgaacag cacatttaga atgtccgctc cgattatatt gcctacaagc
     3421 agttcagggt ggcctttgat tatggagaca atcgcagtca caagctccgg cagagacgtc
     3481 ccgaatgcca cgagcgtcac agccagcacg ctttccggca cgccgtagtg aatgcatatc
     3541 ctggcagttg agccgaccat tatatccgac cccaacagca caagcagaag gcccattgcc
     3601 atcgtaacaa gcattacggc tactgcgccg aactttgatt ttctagtcgg cctgccgtta
     3661 ttttggccgt ccatagcttc ttcgggtatt atttcaggat gcagccgcgc ccaatgggct
     3721 gaaatgccca tgtaacctat cagcagaacg acaaaagaga atcccatcca gcgcggcatt
     3781 acaacgccgt aaaaactgcc cttcatccac gccaaaacca gaaatgatgc tgtaagtagc
     3841 gtccctgctg caagttgcag ccagccctgc cgcacaagca gaaacctgtt cttcggcagc
     3901 cgacacagac agcacgccaa gccgaaaata agcgccgtat cgcagattat cgaccctacg
     3961 ccgttgccca gcgcaaggcc gggattgttg ctgaaggctg cggttacaga tacgaacgtc
     4021 tcaggtgcgg tcgtgcccag tgaaactatc gtcgcgccta tgatgaccgg cgaaaagccc
     4081 aaggaacggg ctatctgcac tgccgccgca accagcttgt cggcgccgta aaccaacaaa
     4141 agaacggaac taagcaccat cagccacagc agccagattc tatccgcatc caactgagcc
     4201 tcaaaaaacg aatccgatat gatttcagct atcatcatgc ggataaaaaa taacttcacc
     4261 aaccatgatt tacaagaaaa taacgttgaa tgaaaaattt ttttcttgtt gaaatcgtaa
     4321 ggaatcgtat attcttattt gcaaaaatta ctcggctgaa aatgtagttt tgaacgaagc
     4381 agccactgaa gctgtaaagt cgaaattttt ctcctccttc gaaaactggc agaaacggat
     4441 gcgttcgttc atgtttttta aaagaccgta aaattacgaa tgatattttt taaaaaaatt
     4501 aaaaaattaa atttaaaaat tttaaaaact tttctatata acagcccctg ctgggagaaa
     4561 tcgaatgtca ttaaaaatca ggtgcagcga atgccacaag aaaatttctg tggatgaagc
     4621 cttcgccgga agcatgtgcc gctgtccgta ttgcaaatca atcgttctcg tacctaagca
     4681 gatatctcgc gccgccgatt ccgtgggccg tccaggcagt cccgcgatac ggccgacgat
     4741 accgactgtc ggtcggccga cggcgcccgg ccagacaaca atctccgcac ctggtcatga
     4801 aacaatgccc tcgcctgttc tcagggagat gaaacaaccc cagccggcgg aggccggcat
     4861 acctggcggc aagagcagtt acatagcatc catggattca aaagccgttg aaaataaaaa
     4921 gccagcccag gaggaaaaag ccgtgcagaa aacctcaaca aaaaaaccgg ctgcaacttc
     4981 cgcaaccgtc gaagaagcag tcgtcgacgc cgtcaaggtc gacaccagca aactcagcaa
     5041 ggaacagata gcagccatcc ccaccgcaaa tccaataatg ttccagggca tagtatcgct
     5101 ggcgctgata gtagttcttc ttgcaatgat aggtgcatgc atcttcctcg gctaccggat
     5161 gttcgcctcg aaggaaagac agaattcgga ttcgatatat tcttcgcagg aaatcgccaa
     5221 agaaattcag gcgacgtcct acgctacgga agatgtcaat ccattcgaat acaataccgg
     5281 cgcttcgctg atatgcaacc agttcaagat aaccgggccg gtagtgttct gcatagacgc
     5341 cggcacatct atgggcgaca aatactgcct ggccagagac atggtccgcg cttctgtact
     5401 cagtatgaaa tcatctgaaa atttcgcagt aatcgtcaat caggacggcg gcacaaacgt
     5461 catcacgccc atgacagccg gcggccctga aggcgaagct aaaatacgtc aaaaactcat
     5521 ggagcaggct gacgacggca cggtagtcct cggcggtgcg ccgatgctgg aagaggcgat
     5581 attgtccgca tccgaattca agcccaaaac catagtgtta ttaattgccg acaaggatat
     5641 agactcccca aaacgtttaa tcgccgacct ccagaaatcc ggcgcagacc tggttataat
     5701 ttcactcgac gccaagcctg gccaggatac gatgataaaa gaactcgcga agaacgccgg
     5761 ccaaaatacg caactgaagt tatataacgt cgaagaactt gaaaaaagtt atgaaaaatg
     5821 caatctgccg gcggactgat tttcatttac cagtcgcaag catattaaaa atcggaaata
     5881 tggcctgtca cgcttttgaa agtttttgtt gtgttgcagc aacggcgatt gcatgggtaa
     5941 ctacctgaac agccgcagct tgaacgtttt tcatgttttt tattcggatt gagattttgt
     6001 cgagggtatt tatacggtgg ctgtgacaag aaaaaggatt gaaggcaatc ttgcggctat
     6061 cagaagtcgg atagctgccg cggcggctcg ctccggccgt gatgctgctg acgtaaaaat
     6121 agtcgcggtc accaaatccg tggacacgga aaccatacgt aatctcatag acgccggcat
     6181 tactgccctg gccgaaaatc gggttcaaca gttcgtgacg cgagcagaag agattaaccg
     6241 atatcttcag cgaaggcgca accctctggc gtcgccagtc gaatggcatc tgataggtca
     6301 tcttcagcgc aacaaggtaa aatccgccgt cgagatttca gccatgatac attcggttga
     6361 ttcgctgcgg ctggcggaag agataaacga acggtgcgag aagatgaatc gtcggctgcc
     6421 ggttctgctc caggtgaact gttcgcagga ggagcagaaa tacggctgtg ccgtcggcgc
     6481 agcgattcac ctgggcgaat tgatggccca gatgaaacac atcgaattat cggggttgat
     6541 gacgatgggg ccgatggacg gcaatcctga cggcacgaga catgcgttcg tccgtttgcg
     6601 tgaactgttt gaagaaatgc gacacgaaaa catcggcggc agagattttc gtcacctttc
     6661 catgggcatg agcggtgatt atgaaatcgc cgtcgaacag ggtgcgacta tggtgcgcat
     6721 cggctcagcc ctttttgagt gacagtctgg ctatcatttc cgtcgcccgc aacacttcag
     6781 ccaccgacca ggcctgcgca aagcagcctc ttggcgtgtg tggcgcatcg ccgtcgaata
     6841 tttcgtttat ctggcccaga caaccttcag ccatgtgttt ttcaaacggc ttcatgagcc
     6901 tggccagttg cgcccgcgac gcgccggtct ttatcgacgc ctcaatataa aagcccatca
     6961 gccacgccca cgccgtcccc tggtgatacg ccctgtcgcg gatatattgc gggccttcgt
     7021 atctgccctt gtatcgccag tcgtccggcg aaagcgtccg caatcccaac ggcgtaagga
     7081 gtttttctct gaccactttc acgatgctct ttatctgtgc cggtttcagt ggcgaatacg
     7141 gcagtgcagc ggcaagaatc tgattaggcc gcagggactt gtccggtcca taaggataaa
     7201 cgcagtccgc caaatatccc gcattctcca gccagaagtt gttaatgaac gcgccggcag
     7261 tcttacgggc tgcgacagca tatttggcgt cgatatcgcc cagcagcgcg ttagcctgat
     7321 accacagtgc gttaagttcg acagccttgc cgtaacgcgg cgtaacggat tcgttgtcta
     7381 ttcgcacatc catccacgta agctgcgtat tttcatcgcc gcccaccagc aggccgtcag
     7441 tgtcctcgtg tattccgaac atcgtgccgg cgcgatactg gtcgactatg gttttgcatg
     7501 ccggcagcaa tacttcacgc tcaaaacatc ggccttcgtg ggaatcatcc gcacgaatgt
     7561 accgcagggc agccaacgta aaccacagcg aggcatcaat gctgttgtaa tgcggcgcca
     7621 agccgtaatc gtcaaaacga ttgggtatca tgccctgcga aatattgtcg gcgtatgttt
     7681 tgaaaacacg gcgggcaagt tcgaatctgc cggtagaaag cagcaggcct ggcagtgcga
     7741 taaacgtgtc acggccccaa tcggcaaacc acggaaaacc ggctaatatg gaagcaccgt
     7801 caaaatcgtc cggcgttttc cggtcaacca gaaacacatt gcccgactga gccaggcatc
     7861 gcatcatgtc cggacatcca gaaacgccgg cgtcgatttc ttcgctctct tccagcagtt
     7921 tattatattc gtccaaaaac ttcacagcca cgttgccgtc cagggaggct acgatttcgc
     7981 agctttcctt ctttttggac ggccgccagg taaaatatcc ggcggacggc agatcttcca
     8041 taaaatcctg gccgcgagcc gcgtcggtac gataataaaa attgaaccac cactgcatgt
     8101 ccggcagaaa ttcagccctg tcgcaactga tgacgagttt gggctgctcg ccagccgtta
     8161 cagccagccg gccattcgaa acctcaaagt caggatgata accggcgttg gccagatgat
     8221 ggaagtcgcg catcggcgta aacggccgaa ttatgatttt ttccgcttcg ccggagagtt
     8281 cataacgcac aataagcgta ttggaatcgc ggcagagcag aacccgcttt ttgagagtct
     8341 ggcggtcgcc tatcctgtaa tcgtactcgg cgattatgcc tttagtgaac gttctcaaat
     8401 cccgccaacc ctgcggcgca aaaacgccgt tggggaactc gttacacgcc agctcgaatt
     8461 taccctgcga cgtataaacc tgttccatca acattgagag agcgactatc cttcccaccg
     8521 gcggattggt cgccgccgtc agcagtgcgt gatatctgcg agtattgcag ccggcagtag
     8581 ttgaagaact gtaagacccc aggcggttcg ccagcaacca ttcacgactc aacagcccct
     8641 cctggcccgc acgttgagcg cgaacaacgt attgattaag tatttttttg cccatttcac
     8701 ttttattata ggcagcctta aatcatttat ccattttcat gatttttttt tgttctgcga
     8761 cagaaaaata cttgcttctt gtgtggggtg tttacccgta attctcgccg aaccataaaa
     8821 aaacacgctt catacctttt ttaaaaaaac gataaacaaa cgtgtctgaa gtgattctcg
     8881 aaagaatttt ttcttttgtg cttcatcggt cagcttaaaa atgttaaagg ggctcggcta
     8941 gacttaaggt tactttccga cctctgtggc agtcgaaccc ctttaaaaaa atatctgcca
     9001 ttttctgacg ccgcaaaaca acaacaagcg caaaggcaag tttaatatgc ttgaataaaa
     9061 cgaaagaaaa agcccgatta tgtatcgggc tcctcttttc catttcttac aaaacagggt
     9121 tttcggaaac aatttttaga acctatccca aaaacatggg cgtcgccgaa atcgagcaat
     9181 tttgcggccc ggcaaggaag aaaaatggca gcatactttt aaaaaaaaca tattctgccg
     9241 tttttctgac gccgccaggc tgcaaaagag ccgattgcag gcagccaagg tttttgggat
     9301 aggttctaat attttctcat aaggcctatg actacacctt gaatttcaca tttatcgact
     9361 acaataggcg caaacgcatc atttgccggc tgaagacgga ttttcccctt ttcgcgataa
     9421 atacgtttaa gggtagtctc gccgtcatta agcagcgcaa ctacaatttc gccgttacgg
     9481 gctgtatttc ttcgctggca gatgacataa tcaccatcca tgatatggtc atttatcata
     9541 ctctcgccgg caacttccag tacgaacgta gaaccatccg ccggaaattc ctcattgaga
     9601 ttcatccgtt cgcaattctc aaacgcttcc agcggcacac cggcgactat cctgccaaca
     9661 aacggcagcg actcttcctg ttcgtccgga aaaacaaagt catccgagac taccaaagag
     9721 cgagccctgt gtttttcgcc gcgtaacagt acgccttttt tttccaacgc gccgacgtgt
     9781 tcgaagactg taaccttggt aagactcaga tgatccccaa tctcctgcat tgttggggaa
     9841 taaccgtgac tcctccgaaa gtcccttata tatcgaagaa tttccagttg ccgtggtgtt
     9901 actgtctgca ttcgtgttct cctgtgtttt caccttttgg tgttttgcga ctgtataatt
     9961 tgatcctgca gtaatacggc aggcatcaac actcgacata gccacttccg catccgcctg
    10021 tgcttcgcac aatatctgcg gatttttcgc cgcggaggag gctgcaacgg cggtagcctt
    10081 cttaccatgc gagcagcttg attgcgactc aacagggttc gcctgcaaca tcgcaatggc
    10141 ttgttccgct cgagctatat gttcggcgac caattcttcg tcgctcttcg cgccgggccg
    10201 gatttcgtgc aacacttcaa gtatcgattt gacgacgcct ttgaggtata tgctcttctg
    10261 ctgccatttt acgccgtaat atcccgtgca aaccaacgcc ggctcaaggc cgaggtcatc
    10321 cgccagtttt gcaagcattc ggggcgaaat ctgttcacgt ttttccaatg tcggcagttc
    10381 ggcatcggac ggatatgcct tgcggaaatc tccgccaggt ccgaactggc ataatggttt
    10441 ctttttcatc tcaaacagcc tttccatgct cgacgccagg gtctttatct gtcgcagacg
    10501 agccgtgcga caaatccgat tctctataaa aaccctattt tgcagctaac cggaagcctt
    10561 acgctccggc atcagaaaca ggaagtatat ccatcttttt cctgttccta tctgctctat
    10621 ctattcccag cgtcaactaa ccgtttaggc ctctgttagt agtatatact cctaacatcg
    10681 gataaactca agcctttctt taaaattttt cccaaaaatc ccaaaaattt tccttacaaa
    10741 attttcagtt ttatcgattt ttaacatcat tgctctatat atggcagtgt ttggacacac
    10801 agccaccatc tatagatata tcggtaagga tgatgaaaga taataaataa atttttgccg
    10861 gcatgaatta atattcacgt aacaacttct gcttatcgta tccgttgtgt atgtacagct
    10921 tgagggggca ttctggagat attgttgacc tcagctagat atcatggtat agaaaattgc
    10981 ttatatccgt gtcggccagc ctcaaaatgt tgaacgctga caaagttgca atagaaaaaa
    11041 ggctgctttc agccgatgag atgtttggag aatgaaatga cattacgaaa gatacgggta
    11101 ctttttttac gtctgggcag tttgccagtc atccttatgg cgatattcgt acggccg
//
LOCUS       DAONFI010000023        11094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6642_length_11094_cov_5.046653, whole genome shotgun sequence.
ACCESSION   DAONFI010000023 DAONFI010000000
VERSION     DAONFI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6642_length_11094_cov_5.046653"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1098
                     /locus_tag="PLK08_01410"
     CDS             220..1098
                     /locus_tag="PLK08_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52183.1"
                     /translation="MEKYLLKTFVPADRFFHLHRAEITWSKLDAGHLHGYAEVFWVEK
                     GDLFHVRNGVGELLHEGDVAWLRAGDSHAMRNTGKKTSIINLAFADSVLSSLERRYYS
                     KSTPAPWKCPLNHRINHLDARQIVKFQNLANVLAFSPQTRLSLDNFLLDLLQTISMNN
                     YHNITPQTDCPEWLTRAINEFAQKQLWNLTQQEFAKKLAGRSVEHVGRTVKKIFGKTT
                     NELLNEYRLEKSAADLCITNKAVLEIAMDSGFDNLGYFYRLFKRHFNESPCRFRKLHQ
                     ALIFTFTSGGKNGNCK"
     gene            complement(1117..1764)
                     /locus_tag="PLK08_01415"
     CDS             complement(1117..1764)
                     /locus_tag="PLK08_01415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPS52184.1"
                     /translation="MNIDAQSSYWDSQIAKEFTAPLDLGILEKYLAHQSVIVDVGCGY
                     GRVCDELANNGYINVKGFDISAAMVNKGRRCGLNLELMEHGRIPLDDESVDGALLFSV
                     LTCIPTDDGQRRLMDEIIRILRPGGYVFISDFPIQSHTRCVERYEKYAERYSIYGVFE
                     LPDGAILRHMSMATLHERVAAFNFCDEWTEDIKSMRGNPIRAWRFIGQKPLCDKS"
     gene            complement(1761..2858)
                     /locus_tag="PLK08_01420"
     CDS             complement(1761..2858)
                     /locus_tag="PLK08_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52185.1"
                     /translation="MSDSRIPVAVIGLGAFGQRTLKALQQCDNVEVVAVADKLSDVAQ
                     NVATDAGVEGYTDNRQMILKKKPLAVFVATPPMAWGELLGFCAQNGVHVWKEAPLARN
                     LGEAVNFVRMFEKAGLKLAVGTQRRFSQSYRKLAESKLSIGDVFLARAHYLFNWGSQL
                     NWRADRVSAGGGALLEVGYHPIDLLIWTLGLPEEVYGANMIARKRNLLDETQGIAGSI
                     HDTDDTASATLKYKDDTMAAVVTSRITGPASEEFSLHGRGGSIVANFETCRLRNPDGD
                     VLDYFESKEPPHEIYVRQVQNFLNSINEGVLHYECSAAENLLTHAVIDAIYLSCQTAQ
                     PESPLEQLRIHGMTPRQCLKFSASALAVEEK"
     gene            2969..3937
                     /locus_tag="PLK08_01425"
     CDS             2969..3937
                     /locus_tag="PLK08_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52186.1"
                     /translation="MKELESLPVEYIEIPTDTHQPVAWTINDYAKLTPRHFDIHTDLE
                     LGIVLRGGSRRWYEGHHMEAFRGQFWMAGLWEPHGMEVIRTNTRHLVLGIKPEFLDIP
                     DPFAAFDWLYCFHLDNRQRPQANTAEEKNFVIEFAEKVIALMEGDSPYWQQKLRIYLQ
                     EFLLYFMERQAKPAEVCQTSDSFGSRRTLLPALLTFERNPHKTITLSDAAHMAHMSRS
                     KFSEMFRQTMGIPFGKYCQRRRLAGAMRDLQATDMKLLAIAKRWGFSDAPHLARAFKT
                     ATKQTPQDYRKSWRQQAVKNTPGCAPRMPGQNNDEPNQKRKSRHLW"
     gene            3999..5297
                     /gene="larA"
                     /locus_tag="PLK08_01430"
     CDS             3999..5297
                     /gene="larA"
                     /locus_tag="PLK08_01430"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPS52187.1"
                     /translation="MLDLKQVNLLYGKTGLTVKVPANAYILECSEVPSLPDIEKTVYA
                     ALANPIESPSLEQMIKKRKPQTVAITISDITRPVPNRVFLPVILNILNRCGIRDSQIA
                     IIVGTGMHRPSTAEEHCYLVGADIMSRVAVVDHHATDVATLKKVCDDPPVSVCRQFAE
                     ADFKIVTGYIEAHFMAGFSGGRKGVCPALVDLQTIRNFHGYGTLCSPYADTGNLQNNP
                     CHEIALKVAHAVGVDFLFNVAINNKREIAGIFCGELNAAHLAGCKQVAAWTTAKTDGP
                     YDLVITNGGGYPLDQTFYQTVKGMCTALPAIGEHTVLLQASACNEGLGSKEYANTILR
                     YGHQWQKFLSDISDSRDFTVDQWQFQMQTRVISRIGIKNLWFVTDGIEANILEKLCLT
                     PVYDKGNASTKLQQVVDRFVSDNPCARIAVIPEGPYTILK"
     gene            5360..5671
                     /locus_tag="PLK08_01435"
     CDS             5360..5671
                     /locus_tag="PLK08_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52188.1"
                     /translation="MIKKEPTLEQLRKAIRQLDDELAGKADPLLSFVPREAFITYREY
                     LREMLDIIETDKLPAILPKQYDEMSADIIANWPFTFLGMGIVEAQRAFGDYYNQEAAR
                     R"
     gene            5668..6486
                     /locus_tag="PLK08_01440"
     CDS             5668..6486
                     /locus_tag="PLK08_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPS52189.1"
                     /translation="MRFLSCIVILFATLCACGCSELVVIKTNPSGAEIYCNGERAGVS
                     PCNVPLPESEGDMVGMNVIEARKDGYLPEYYYLSGKPSDETVGHIQTYSLARPTVEIE
                     LTKLPENMTSADVPDVVKSDSESMTVLPGAYENRATIEVRVVRVKDGRVLGQISRYED
                     IGNPTAVAQSIVGELCEYLPPADDKPEIRVVPLKNRRHSDFGKKLSENISEEIMRETC
                     FSGGYPKVQTLELNGVVDEDKLDTQATFNKIKKKGLLYGVDYVLMGGVAESIKP"
     gene            6663..6926
                     /locus_tag="PLK08_01445"
     CDS             6663..6926
                     /locus_tag="PLK08_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014243689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPS52190.1"
                     /translation="MKKGIHPEYRPVVFVDVASGTKYVTRSTVKSKDKVTVDGVEYPM
                     VKVDISAASHPFYTGQQKFLDTAGRVEKFTKKFGGEYFKKRKK"
     gene            7035..8132
                     /gene="prfA"
                     /locus_tag="PLK08_01450"
     CDS             7035..8132
                     /gene="prfA"
                     /locus_tag="PLK08_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPS52191.1"
                     /translation="MADTLIDKLDELDRRFEEIFGQMNNPAVAADGQKIVALSKEHAS
                     LAKIVIPYRKFRQLAKNCDEAQAIQNDPAADAELKELAETEVAELRLAVNNALEEVKG
                     LLVMSEEQEIGSVIMEIRAGTGGDEAALFAGDLLDMYRHYAEQRGWKTEIMDASPTEM
                     GGFKEVVVNIKGESVWSRLGYEGGGHRVQRVPKTENQGRIHTSAATVAVLPEPEELDI
                     QIDWEKDVLEHVSRAGGPGGQNVNKVSSAIKLEHLPTGITVSMRDEKSQHKNRAKARQ
                     ILTARVYEHFRSQQASAESAARKEMIGSGDRSQRIRTYNFPQNRCTDHRLKGEGQEGA
                     NFNLERVLAGDLDPIVDALIAHDKAQRLANL"
     gene            8281..9342
                     /gene="queA"
                     /locus_tag="PLK08_01455"
     CDS             8281..9342
                     /gene="queA"
                     /locus_tag="PLK08_01455"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPS52192.1"
                     /translation="MSTKQFDYHLPAELIAQHPSDDRAASRLMHLTRSDGKINHRTFA
                     DIVELLQPGDLLIVNNTRVIPARFYCRRESGAQIEGLFVKYLADNCWEVMLKNATRCK
                     EGETVFFRNDELQGLRLMQRLGQGLWKVLPQPAGLAEEILDRLGRTPLPPYIHREQLS
                     PDEEDRDRYQTVFARTPGAVAAPTAGLHFTQKLLEKLQARGISMADVTLHVGLGTFLP
                     VKVDDASKHEMHSEWYEISAQTAEAIGSAKRDGRRVIAIGTTSVRVLESVTRRFDKKI
                     QPCSGETKLFLYPSAEFYAVDAMITNFHLPQSTLLMLVAAFCCPGGNKGIQMILDAYA
                     QAVQQQYRFFSYGDAMFIE"
     gene            9339..10235
                     /locus_tag="PLK08_01460"
     CDS             9339..10235
                     /locus_tag="PLK08_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPS52193.1"
                     /translation="MTRILVTSDLHYDVKRSQDAAERLATHACKTGGDAIVLAGDTAG
                     ADLEKFSQALALFKNFSGQKLLVPGNHCLWCNPIGIDGLPPDASIERYEKLIPQVAEE
                     HGFAVLDQQPLVMDGTGLVGSVGWYDYSMRCESLGIPLDFYRIKLSPGAARYMGGYET
                     LLEKYGNILTQEHMSLGVRWMDGFRVRLGMSDEAFLNSLLAKLKRQLEEISRHADRII
                     AFIHHLPFMELVPQKRPMRFAFAAAYLGSIRIGQLLRNFENVTDIFCGHSHWPAKYND
                     GKIIATAMGNTYLEKRLTIVEC"
     gene            complement(10264..>11094)
                     /locus_tag="PLK08_01465"
     CDS             complement(10264..>11094)
                     /locus_tag="PLK08_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52194.1"
                     /translation="TNRIYSYDRRMFRFDCGQSELDVPFSAIDWINTYQPVGRIWCDF
                     GSTSNIWFFVKSRPQVPGFSCTFACQPSCLTENEEYVRGKKSFDEACYKYDVQIVVLR
                     TSDFNRPLLEKLSRDKKFCLVDLDVRQALFIRNDGPNAILARQKRITPENFDVRKYIA
                     YLRESSPTRPAINLHLGASTLERMGWYDQAIEVFEELIAEDKNMPEAYFELGATLALR
                     GMKYESAGKNEEALHDFRSAKEFFEKTLQINPSFTYATYNLRIAKDDIRRLEILTGER
                     "
BASE COUNT         3070 a         2765 c         2644 g         2615 t
ORIGIN      
        1 catgggcgtc gccgaaatcg agcaattttg ctggctggca aggaagaaaa atggcggcat
       61 atttttttaa aaaaatattc tgccgttttt ctgacgccgc caggctgcaa aagagccgat
      121 tgcaggcagc caaggttttt gggatagatt ctagtataaa ttatatttat ttgatattct
      181 tgcacgatat tgaacgtttt tttatactat actgacataa tggaaaaata tctgcttaaa
      241 acgttcgtac ctgcggacag attttttcat ctgcacaggg cggaaataac atggtcgaaa
      301 ctggacgcag gccatctgca cggctatgcg gaggtttttt gggttgaaaa aggcgacttg
      361 ttccacgtcc gtaatggggt aggcgagctg cttcacgagg gcgatgttgc atggttacgc
      421 gcaggcgatt cacacgctat gcggaatacc ggcaaaaaaa cctcgataat aaatctggca
      481 tttgctgatt cggtgttgtc ttcacttgag cgacgttatt attcaaagtc gactccggcg
      541 ccgtggaaat gcccgctaaa tcatcggata aatcatcttg atgccaggca gattgtaaag
      601 tttcaaaacc tcgccaacgt attagccttc agcccgcaaa cccgactctc tctggataat
      661 tttctactcg acctgctgca aaccataagc atgaacaatt accataatat tacgccgcaa
      721 acagattgcc ctgagtggct gacacgggca atcaacgaat tcgcacagaa acagttatgg
      781 aatttgacgc agcaggagtt tgcgaaaaaa ctggcgggca gatctgtcga acacgtcgga
      841 cggactgtta aaaaaatttt tgggaaaacc acgaatgaac ttctcaacga atatcgtctg
      901 gaaaaatcag ccgccgattt gtgcataacg aacaaagccg tactcgaaat cgccatggat
      961 agcggctttg acaacctggg ctatttttac aggctgttta aacgacattt taacgagtct
     1021 ccctgtcgtt tcagaaagtt gcatcaggcg cttattttca ctttcacttc gggcggcaaa
     1081 aacggcaact gcaaataagg ccgccaaaga gaattttcaa gatttgtcgc aaagaggttt
     1141 ttggcctata aaccgccagg ctctgatagg attgccacgc atcgatttta tatcttcagt
     1201 ccactcatcg cagaaattga aagccgccac gcgctcatga agcgtcgcca tgctcatgtg
     1261 ccgcaatatg gcgccgtctg gcaattcgaa gacgccatat atcgaatatc gttcggcata
     1321 tttctcataa cgctccacgc atcgggtatg cgactgtatc ggaaaatcgc ttatgaaaac
     1381 atatccgccc ggccgtagaa tacggattat ttcatccata agtctacgtt ggccgtcatc
     1441 tgtcggaatg cacgtcagta ccgaaaaaag cagtgccccg tcaacagatt cgtcatcaag
     1501 cggaatcctg ccatgctcca taagctccag attcaacccg caacgacggc ctttattcac
     1561 catcgccgca gaaatatcaa aaccttttac attgatatac ccgttattgg caagttcatc
     1621 gcaaacgcgg ccatatccgc agccgacgtc gactattacg ctctggtgag cgagatattt
     1681 ttcaagtatg cccaaatcaa gcggcgcggt gaattccttg gcaatctgcg aatcccagta
     1741 ggaactctgc gcgtcgatat tcatttttcc tccaccgcca gagcggaagc tgaaaatttg
     1801 agacactgcc gcggagtcat gccgtgtatc cgcagttgtt ccagcggact ttccggctga
     1861 gctgtttgac atgaaagata tatggcgtca atcactgcat gcgtaaggag attttcagct
     1921 gcggaacatt cataatgcaa gacgccttca ttaatggaat ttaaaaaatt ctgtacctgc
     1981 cggacgtaaa tttcatgcgg cggttctttg gattcaaaat agtcaagcac gtcgccatcc
     2041 ggatttctca ggcggcaggt ctcgaaattc gcaactatcg acccgccgcg gccatgcagt
     2101 gaaaattcct cgctcgccgg gccggttatt ctcgacgtga ccacagccgc catcgtgtca
     2161 tccttgtatt tcagtgtcgc cgaggcggta tcgtcggtat cgtgaatcga acccgcaatg
     2221 ccctgcgttt catcgagcag gtttcgtttc ctcgcaatca tgtttgcgcc gtacacttct
     2281 tccggcaggc cgagcgtcca tatcagcagg tctatcggat gatagcctac ttccagcagt
     2341 gcaccgccgc cggctgatac tctgtcggct cgccagttaa gctggctgcc ccagttaaac
     2401 agataatgcg cacgcgccag gaaaacatcg cctatgctga gtttgctttc tgcaagttta
     2461 cgatatgatt gcgagaatcg gcgttgtgtg ccgacggcta gtttcaggcc ggctttttca
     2521 aacattcgca cgaaattgac agcctccccc aaatttctcg ccagcggagc ttccttccag
     2581 acgtgaacgc cattctgtgc gcaaaatccc aacaactcgc cccatgccat cggaggagtt
     2641 gcgacaaaaa cggcaagcgg ttttttcttc aaaatcatct gccgattatc ggtataaccc
     2701 tcaactcccg catcggttgc gacattttgc gccacatcgg aaagtttgtc cgccaccgcc
     2761 acgacttcca cattgtcgca ctgttgaagt gccttgagcg tacgctgtcc gaatgcgccc
     2821 aaccctatca cggcaactgg aattcttgag tcactcatat tgcaagagta tcccactcaa
     2881 atcaaaattt gtcaatctca aaattgaata atcgtccacg atatgatata cttcctccac
     2941 cggtgcagca gccgtgaggt atcgccacat gaaggaactg gaatccctgc ctgtagaata
     3001 tatcgaaatc ccaaccgaca cgcatcagcc tgtggcgtgg acgattaacg attatgccaa
     3061 actcacgccc aggcatttcg acattcacac cgaccttgag ctgggcatag ttcttcgcgg
     3121 cggcagcaga cgatggtacg aggggcatca tatggaagcg ttccgcggcc aattctggat
     3181 ggctggcctc tgggagccgc acggcatgga agtcatccgc acgaataccc gtcatctggt
     3241 actcggcatc aagccggaat ttctggacat ccccgacccg ttcgccgcat tcgactggtt
     3301 gtactgtttt catctcgaca acagacagcg gccgcaggct aataccgcgg aagaaaaaaa
     3361 tttcgtcatc gaattcgcgg aaaaggtaat cgccctgatg gaaggcgaca gcccctattg
     3421 gcagcagaaa ttgcggatat atctccagga atttctgctg tacttcatgg agcggcaggc
     3481 gaaacccgcc gaggtctgtc agacgtccga cagcttcggc agccgccgaa ccctgctgcc
     3541 tgcgctcctg acgttcgagc gaaatccgca caagaccatc actctcagtg atgcagccca
     3601 tatggcgcac atgtcgcgtt cgaaattctc cgaaatgttc aggcagacca tgggcatacc
     3661 attcggcaaa tattgtcagc ggcggcggct cgccggagcc atgcgggatt tgcaggcaac
     3721 ggacatgaaa ctcctggcca tcgccaagcg atggggattt tctgacgcgc cgcacctggc
     3781 tcgcgcattt aaaacggcta ccaagcagac gccgcaggat tatcggaaat cgtggcggca
     3841 gcaggcagtc aaaaacacgc caggctgcgc gccgagaatg cccggccaga ataatgacga
     3901 accgaaccaa aagagaaaat cacgacatct ctggtaattt tgaaatttaa catttgaata
     3961 ttctgaatta atatgagatc tgggaaaaag aggttattat tttagactta aaacaagtta
     4021 atttattata tggcaaaaca ggattgaccg tgaaagtgcc agctaatgca tatattttag
     4081 agtgttctga ggttccgtct ttgccagata ttgagaaaac ggtttatgcc gcactcgcta
     4141 atccgattga atcgccatcg cttgagcaaa tgataaaaaa acgcaagccg caaaccgttg
     4201 ccattacaat ttccgacatt acccgccctg tgcccaatcg tgtttttctt ccggtaattc
     4261 ttaacattct taacagatgc ggcataagag attcacagat tgcgattatc gtcggtactg
     4321 gtatgcatcg gccaagtacg gcagaggaac attgttatct tgtcggagca gatataatgt
     4381 ctcgcgtagc agtggtagac caccatgcga cagatgttgc tacattgaaa aaagtatgtg
     4441 atgacccgcc ggttagtgta tgcagacagt ttgcagaagc tgactttaaa attgtaacag
     4501 gatatattga agcccatttt atggctggtt tttccggcgg acgaaaaggc gtgtgtcctg
     4561 cacttgtgga tttgcagacg attcgtaatt ttcatggata tggcacatta tgttcaccat
     4621 atgcagatac gggaaacttg caaaataatc catgtcatga aatcgcttta aaagtggcac
     4681 atgcagtagg tgtggatttt cttttcaacg ttgcgataaa caataaacgc gaaatagcag
     4741 gcattttttg cggcgaattg aatgcggctc atctggctgg ctgtaaacag gttgcggcct
     4801 ggactacggc aaaaactgac ggcccgtatg acttggtgat aaccaatggt ggaggctatc
     4861 cgctggatca gacgttttat cagacggtta aaggcatgtg tactgcactg cctgcgattg
     4921 gagaacatac cgtattattg caggctagcg catgcaatga aggcttgggt tcaaaagaat
     4981 atgcaaacac gatattgcgt tatggccatc aatggcaaaa atttctgtct gatatttctg
     5041 atagcaggga ttttaccgtt gaccaatggc aatttcagat gcagacgcga gtgataagtc
     5101 gaataggtat taagaacctg tggtttgtta ccgacggaat tgaagcaaat atactcgaaa
     5161 aactttgttt gacacctgtt tatgacaaag gcaatgcgag cacgaaatta caacaggtcg
     5221 tggataggtt tgtatccgat aatccgtgcg cacgaatagc cgtaatccct gaaggtccat
     5281 atacaatttt aaaatagccg aagacttatg aaaaagactg aatctggagc atgttaaatt
     5341 tttaaacatg gaacgataca tgataaaaaa agaacccaca cttgagcagt tacggaaggc
     5401 tatccggcaa cttgacgacg aactcgccgg caaagccgac ccgctccttt cgtttgtgcc
     5461 gcgggaagcg ttcattacat atcgcgaata tctccgcgaa atgctcgata tcattgaaac
     5521 cgacaaactg cccgcaatac tgcccaaaca gtacgatgaa atgtcggcag acataatcgc
     5581 aaattggccg ttcacctttc tcggcatggg aatagtagag gctcagcggg catttggaga
     5641 ctattataat caggaggccg cccgcagatg agatttttga gttgcattgt cattttattc
     5701 gccacgcttt gtgcctgcgg ttgtagcgag ctggtcgtaa tcaaaaccaa cccgtccggc
     5761 gcggaaatat attgcaacgg cgaacgcgcc ggcgtcagtc cgtgtaacgt accgctgccg
     5821 gaaagcgaag gcgatatggt aggcatgaac gtcatcgaag cgagaaagga tggttatctg
     5881 ccggaatact attatctgtc cggcaaaccg tccgatgaaa ccgtcggcca cattcaaacc
     5941 tattcgttag ccaggccgac cgtagaaatc gaactcacga aactgcctga aaatatgaca
     6001 tccgctgacg tgcccgacgt agtcaaatct gactccgaat ccatgacagt tctgccaggc
     6061 gcatacgaaa acagggcaac catcgaggtg cgggtagtgc gggtaaaaga cggcagagtg
     6121 ctgggacaga tttctcgcta cgaggacatc ggcaacccta ccgccgtcgc acaatccata
     6181 gtaggcgaac tctgcgaata tctgccgcct gcggatgaca agccggaaat tcgcgtcgtg
     6241 ccgctgaaaa acagacgcca cagtgacttc ggcaaaaaac tcagcgaaaa tatttctgaa
     6301 gaaataatgc gggaaacatg tttttccggc ggctatccaa aagttcagac gctggaactc
     6361 aatggcgtgg tagacgagga taaactcgac acgcaggcga cttttaacaa aatcaaaaag
     6421 aagggcctgc tttacggcgt cgattacgta cttatgggcg gcgtagccga aagtattaaa
     6481 ccataaaaca cacccgccgg attttttgcg acgtccgtgc cagctacata ttcaaattct
     6541 tcgggcatac gctaaaaaaa ccttgctttc agcggaattt tggttaatat accaatcctt
     6601 ttatgggcga ttgcgggcag aggcgcgctg cataccgccc caaaattgca aggagttttt
     6661 aaatgaaaaa aggcatacac cctgaatatc gtcctgtcgt attcgttgac gtggcatccg
     6721 gcacaaagta cgtaacccgc tcaactgtaa agagtaaaga caaggttacc gtagatggcg
     6781 tagaatatcc gatggtcaag gttgatattt cagccgcatc gcatcctttc tataccggcc
     6841 aacagaagtt tttggacacc gccggccgcg tagaaaagtt caccaaaaaa ttcggcggcg
     6901 aatatttcaa aaaacgcaaa aaatagtttc cgtaaaatcc aaacgccgac ggatggggta
     6961 aaaaactcat aataacccct gccgccggcg tttttacatt tacacgatat caatgtccgt
     7021 cgagcaaaac ccaaatggct gatacgctta tagacaaact ggatgaactt gatcgccgct
     7081 ttgaggagat ttttggacag atgaacaatc cagccgtcgc agctgacggc cagaaaatcg
     7141 tcgccctgtc caaagagcat gcctcgttag ccaagattgt cataccgtat cgcaagttcc
     7201 ggcagctagc caaaaattgt gatgaagcgc aggctatcca gaacgacccc gccgccgatg
     7261 cggaacttaa ggaacttgcc gaaactgaag ttgcggaact ccgtcttgcc gtcaacaatg
     7321 cgttggaaga ggtcaaaggc ctgctcgtaa tgagcgaaga acaggaaata ggttcggtca
     7381 taatggaaat ccgtgccggc accggcggcg acgaagccgc acttttcgcc ggcgacctgt
     7441 tggatatgta tcgtcattat gccgaacagc gcggctggaa aacggaaatc atggatgcca
     7501 gtcccactga aatgggcggc ttcaaggaag tggtggtcaa catcaagggc gaatccgtct
     7561 ggtcacgtct gggctatgaa ggcggcggcc atcgcgtaca gcgagtgcca aaaacagaaa
     7621 atcagggacg catccacaca tccgcagcca ccgtcgccgt actgccagag ccggaagaac
     7681 tcgacatcca gattgattgg gaaaaagacg tcctcgaaca cgtaagccgc gcaggcggcc
     7741 cgggcggcca aaatgtcaac aaggtttcgt cggcaatcaa acttgaacat ctgccgaccg
     7801 gcataacagt ttcaatgcga gatgaaaagt cgcaacacaa aaatcgcgcc aaggcgagac
     7861 agattctgac ggctcgcgtt tacgagcatt tcagatcgca gcaggcgtcg gcagaatccg
     7921 cggcaagaaa agaaatgata ggctctggtg accgctcgca acgaatcaga acttacaact
     7981 tcccgcagaa tcgttgcact gaccatcgtc tcaagggcga aggccaggaa ggtgcgaatt
     8041 ttaatctgga acgcgtatta gccggcgacc tcgaccctat cgtcgatgcg ctgattgctc
     8101 acgataaggc acagcggctg gcaaaccttt aaaaaaacaa accgcatcag ccaaaactgt
     8161 acctccatcc gtacgcaaaa tgaaacaact gccagaaaat tcatacaaga ttgcccgact
     8221 tctgaaacaa ttatatttgt ctgcatccat agccaaccag gaacactggc gactgatttg
     8281 atttccacaa aacaatttga ttatcatctg ccggcggaac tcatcgcaca acatccctcc
     8341 gatgaccgcg ccgcgtcacg tctcatgcat ctgacacggt cggatgggaa aatcaaccat
     8401 cgtacttttg cagacatcgt cgaacttctt cagcccggcg acctgctcat tgtcaacaat
     8461 acccgcgtaa ttcctgcccg cttttactgt cggcgcgagt caggcgcgca gattgaagga
     8521 ttgtttgtaa aatatcttgc cgataactgc tgggaagtga tgctcaaaaa cgcaacccgt
     8581 tgcaaagaag gcgagacagt ctttttccgg aatgatgagt tgcaaggctt gcggctcatg
     8641 cagcgactgg ggcagggcct gtggaaagtt ttgccgcaac cagccggcct ggcggaagaa
     8701 attcttgacc gcctcggcag aactccattg ccgccgtaca tacaccgtga acaactctcg
     8761 ccagacgaag aagaccgcga cagatatcag actgtttttg ccagaacgcc aggcgccgta
     8821 gccgcaccaa cggcaggcct ccacttcacg caaaaattac tcgaaaaact ccaggcgcgc
     8881 ggcatatcaa tggctgacgt aacactacac gtaggattgg gcacatttct gccggtaaaa
     8941 gtcgatgacg cctcaaaaca cgaaatgcac agcgaatggt atgaaatatc cgcacaaacg
     9001 gcagaggcaa tcggttcggc aaaacgcgac ggccgacgtg ttattgccat cggcacgacc
     9061 agcgtgcgtg tgcttgaatc cgtaaccaga agatttgata aaaaaattca gccatgcagc
     9121 ggcgaaacaa aactcttcct ctacccgtcg gcggaatttt acgcagtcga cgcaatgata
     9181 acgaatttcc atctgccgca aagtactttg ctaatgcttg tagccgcatt ttgttgtcct
     9241 ggcggaaata aaggcataca aatgatactc gacgcatatg cacaggcggt gcaacaacaa
     9301 taccgctttt tctcatacgg cgatgccatg ttcatagaat gacgcgaata ctcgtaacaa
     9361 gcgatttgca ttatgacgtc aaacgttcgc aggacgctgc ggaacggctg gcaactcacg
     9421 cgtgcaaaac cggcggcgac gcgattgtac tggcaggcga tacggctgga gcggatttgg
     9481 aaaaattctc gcaggcgctt gcactgttca aaaatttttc cggccaaaaa ttactcgtgc
     9541 caggcaacca ctgcctgtgg tgcaatccta tcggcattga cggattaccg cccgacgcct
     9601 ctattgaacg atatgaaaaa ctcatcccgc aggttgcgga agagcacggg tttgcagtgc
     9661 tcgaccaaca accactcgta atggacggaa caggtcttgt cggctcggtg ggatggtacg
     9721 actattccat gcgatgcgaa tcactcggaa tcccgctgga tttttatcgt atcaaacttt
     9781 cgccaggcgc ggctagatac atgggcggct acgaaactct tttggaaaaa tacggcaata
     9841 tattgacgca ggagcatatg tcattgggag taagatggat ggacggtttt cgagtgcgac
     9901 tgggaatgag cgatgaggct tttttgaatt cactgctcgc caaacttaag cgccaactgg
     9961 aagaaatttc gcgacacgca gatagaatca tcgcattcat tcaccacctt ccgttcatgg
    10021 aattagttcc gcaaaaacgt ccgatgcgat ttgcttttgc ggcggcctat ctcggctcga
    10081 tcaggatagg tcaattattg cggaatttcg aaaatgttac ggatatattt tgcggccatt
    10141 cacattggcc ggcgaaatat aatgacggca aaataatcgc cacggcaatg ggtaatacgt
    10201 atctggaaaa aagactgact atcgtagaat gttgaattta cggctgccgc gaaaaatgca
    10261 aaattatctt tcacccgtca aaatttcaag acgacggatg tcatctttgg ctattcgcag
    10321 attatacgtc gcataagtga aagacggatt tatctggagc gttttttcaa aaaattcttt
    10381 tgcactgcgg aaatcatgga gagcttcttc atttttgccg gctgattcat atttcatacc
    10441 tctcaacgcc agcgtagcgc caagctcgaa atacgcctca ggcatatttt tatcttccgc
    10501 aatcaattcc tcaaaaacct ctatagcctg gtcataccac cccatgcgtt caagagtaga
    10561 cgcacccaga tgcagattta tcgccggccg cgtaggactg gactcccgca ggtaggcaat
    10621 atattttctg acatcaaaat tttctggggt aattcttttt tgtctggcaa gaattgcatt
    10681 gggaccatca ttgcggatga acaatgcctg tctcacatcc aggtcaacaa gacagaattt
    10741 tttatccctt gaaagttttt ccagcaaagg cctgttgaaa tcgctcgtgc gcaatacaac
    10801 gatttgaaca tcatatttgt agcacgcttc atcaaacgac tttttccccc tgacatattc
    10861 ttcgttctcg gtcaggcaag acggctgaca ggcaaaggta catgaaaaac ccggtacttg
    10921 cgggcgagat tttacaaaaa accagatatt gcttgtcgaa ccgaaatcgc accagattcg
    10981 tccgaccggc tgataagtgt taatccagtc tatcgccgaa aacggcacgt caagttccga
    11041 ttgcccgcaa tcaaaacgaa acatgcgacg atcatagcta taaatcctat tagt
//
LOCUS       DAONFI010000024        11082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6649_length_11082_cov_4.478643, whole genome shotgun sequence.
ACCESSION   DAONFI010000024 DAONFI010000000
VERSION     DAONFI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6649_length_11082_cov_4.478643"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLK08_01470"
     CDS             complement(<1..859)
                     /locus_tag="PLK08_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPS52195.1"
                     /translation="MKVISIINQKGGVGKTTTVANLAAAMAAAGRKVAVIDLDPQSHL
                     TLHFGFEPADDTATIYDVLCEQATMDEAMVKVRDNLWVVPATTDLAAATAELVSRPNR
                     NMILRNAIDAMKSHVDFMMIDCPPSLGLLTLNALAATDDVIIPLQPQFLALQGLSKLL
                     RTIELVNQRINPKLRVAGVVFCMFEQVTRLARDVVTDVMKFFKSTRTSGKPWADACVY
                     HTAIRRNIKLAECPSFGQSIFEYDAQCNGARDYRALADEFLNRIAAVQPESTVPADMP
                     SITQEHLGAE"
     gene            complement(877..2928)
                     /locus_tag="PLK08_01475"
     CDS             complement(877..2928)
                     /locus_tag="PLK08_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPS52196.1"
                     /translation="MRKNVLTIGLMIFYSGICFAGQAWWETAWPLRVNVSLDEEFTAS
                     QLPGDDVGVVEFHTGGKTRDDGGDVRVCTADNVQVRHKVLCCGPGDLIRVAFAPVSGV
                     KDYYVYYGNQSAVAESSLDIKRGLMLESWRYVGGNADAYPQATITLEKSKKNPKNFVG
                     CGFVPRIFSAINPFTGNYSLINVYTGWFVAPQSGSYLFIISSNNASFLSIDSKLLLET
                     PAWHGPVRQPTIRRNVSLTAGLHRMDVLQVTPRGYPTMAVYWRQPGESGFEVMPAQFF
                     TRIYSATAGLPERYGLPGTLDIRVNERGEAVVDNRHIQRRVFEVVGSDADGEGWQWDF
                     GDGQTGAGRKVSHVYLHDGVYAVTAKKGFDVITQRYYVSRDWPMVMRDVAEEPAGYGK
                     IVGDYDLSAVSATDLMTAVELFKMAGNWDGMMRLCEMFVNRPNITARELESVLGTYCD
                     MLILAGQAGRAAQALEKLAAGRKHGDAAVALYVRAGDIYLYELDDVDAAVRCYRFGRQ
                     DDDVPQKMSRREIIAAGDACMAAGDYSQANKYYMRAGLSGDLVKKNPVTARGNFARQA
                     EDYLHRGEYELLADVLEKWEDAIPTDRLTGYLTLLRVRMFGEQGRYVRAVNCAARLVN
                     VNPQSQYAPQLLLAGAEYAQKNSQPQRAAELLEKLLKNYPESPLCDNAKKLLNQWHNS
                     Q"
     gene            3001..3615
                     /locus_tag="PLK08_01480"
     CDS             3001..3615
                     /locus_tag="PLK08_01480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPS52197.1"
                     /translation="MLETGVKTSKYDGVIFDMDGTLVESSLDFDVLRRELEMSPGRGI
                     LEQINEMPPSRQSTAHSKLLEFELAGARNAILNDGAEEILNELLSRRIPIALLTRNTR
                     QSFEIVSKKFPIMKNFDIVMCREDGVIKPEPDGIILACRKMGIVPQKTLCVGDFEFDM
                     LAARAAGAIGVLLTTHPNWKNYADKADYIINDLPALLNIFDSRD"
     gene            3662..4876
                     /locus_tag="PLK08_01485"
     CDS             3662..4876
                     /locus_tag="PLK08_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPS52198.1"
                     /translation="MNLPLANILHNKMRSLFCVTGIGLGVCMIITLAGLSRGSLYEIA
                     DRMNCVDADVIIVPPGLADKTPTLSGIAISEKVSDILMQKYPDVIARCAAVCIGSLKL
                     GGQDQRVMGIDPRDWDMITNRAELSGGRLFDPDNRFSAWLTEQLLKPAEDDDDNGELL
                     NLSDRLASHGGLEIVIDSRLAAAGRYRLGQMVHAGNHDWKIVGIVPAGVPTRVLTPIR
                     TAQYLFGFGDIKKCTMIFVKFKPGIDAAPLAAQISKQMKLDVLPMSQYRTMLMDRFGV
                     MFSYIDAVNAVALIIAFLIVTVTLYMMVLQQTREIAIMKSCGAGGTFILRQVMGEALL
                     LTLAGMAVGVGMSFPAALAISHFRPLLTVIITPGWILTASAAALVGAVVSALYPAWRA
                     SKVDMLEALTYE"
     gene            4869..5543
                     /locus_tag="PLK08_01490"
     CDS             4869..5543
                     /locus_tag="PLK08_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS52199.1"
                     /translation="MNDPEKHILSAVGLNKSFGRGEAANHVLRGIDIGVNAGEFVAVM
                     GPSGCGKSTLLHILGLMTSADSGEIFYNGQAVTHSESSRRKLRRNHIGFIFQRFNLIG
                     TLSAADNIRISLRIRGLKDDGRSDELLKLMHVSHVARRRPGQMSIGEQQRVAAARALA
                     HKPAILFADEPTGNLDSENGLALLELFKKVNTELGQTIVMITHSNQAAQWAHRIIRMK
                     DGKIDG"
     gene            5536..6294
                     /locus_tag="PLK08_01495"
     CDS             5536..6294
                     /locus_tag="PLK08_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52200.1"
                     /translation="MDDVTAKSEYSHIRVRWWFAAFAVAFAIGVCWLAWLIKTQQLQF
                     STWGEFKSAVITASPLLKLLVLGLYLSICCTFLPLNTSWIISAAAMQGTAVAGNMWQT
                     VLLVSLVGAAASTVANLNDYHLFTVMMRYHHIAKIRHTRTYAVAVKWFERSPFALLLL
                     FNVLPIPVDVVRPLAAAHKYRRTKFAAANFIGRFARYAVIAVITYMLGRQGWIATLAL
                     LAAAVILALPKLAGKILAGRKHNLPESTDEKISA"
     gene            6275..7066
                     /locus_tag="PLK08_01500"
     CDS             6275..7066
                     /locus_tag="PLK08_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPS52201.1"
                     /translation="MKKYQLECMVLAAVVSAVASCPAQQCQPTCSPQEAVSPQTKAIL
                     NRLQAAGDRYTTLTADVNYVVEMRMTGETETRDGWVAFQKNTETTPDMFRIHFDTLKL
                     DQGKKTASKLDYIFDGQWLTVAKHNIKSMTRFQVAKPGEKIEALRIGKGPFPIPFGQK
                     TDDMIKCLQITTRDAEPDDPANTDYLKLIPRKNYEDQLNFKKMEIWIDRETNLPVKVV
                     VRDRNKNVTTAEFSNIKTQSAIDPSLFQMEKPAGWDLTIERIKQN"
     gene            7082..9895
                     /gene="polA"
                     /locus_tag="PLK08_01505"
     CDS             7082..9895
                     /gene="polA"
                     /locus_tag="PLK08_01505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPS52202.1"
                     /translation="MSEKFYIIDGHSQIFRAYYAPFRDLTSPAGEPTRATYVFTNMLL
                     KFIADKKPDYLAMAIDGPHEKLNRTKIFKDYKATRKPTPEDFKPQEKRILQIVKILGI
                     PILDAEGYEADDILATLADRYASENVDVILVSRDKDLDQLINDHVMLYDPMKDETLDA
                     ASIEQSKGYPPSLAVEIQTLMGDNIDNIPGVPGVGPKTAVKLVRQYGSADAVLAAADE
                     QTPKLRDSLKTLGSDRLATSRKLVTLDKHVPMKTDLPEFRFSQPDMQNASKIFMELGF
                     TKLVEKYAAEKPAPKSAAKNDPFIGGLFASLEQSAASSPVAAATTDIKKSQPAMPDGF
                     TTAANFDYQLIDTQDGLDRLAASLRDVKRLAVDTETTGIKAMNCSLVGISLAWKAGHG
                     VYIPVMGPLGATVLDIGKIRDAIGPAMADATVEKIGHNLKYDLIVLENNGFTFAGKMF
                     DTMIAAHVIDSQHASLKMDYLSLEYLHHRCIPITDLIGSGRNQTTMNTVPTDTVAVYA
                     AEDADVTLRLADAMEKILDEMALRNLFENLEMPLMPILTEMERNGITVDPARLKAMQS
                     EFSMKADVLRDRIIRLAGVNFNLESPKQLAEILFVKMGYPAIKKTRTGMSTDSEVLEQ
                     LASEHDCEIAALLLEYRKLTKLISTYLVSLVDCINPRTGRVHTSFHQASVITGRLSSS
                     DPNLQNIPVHDEGRQIRSAFIAKHGCKLISADYSQVELRMLAHFCGDETLCRAFADDL
                     DIHRIVASEVFGVDVDDVTPAMRSRAKTVNFGIIYGQTGFGLARTLGIGRTEASEFIR
                     KYKQRFPKIEVFMAECVEQARNKGYVETISGRRRRIPDIDSRNQQKRNLAERTAINTV
                     VQGSAADLIKQAMINLDRTLEKQNSPAKMLLQIHDELIFETPAESAESTAKVIAHEMS
                     TAIKLNVPLKVDCNIGDNWLEAK"
     gene            complement(9965..11065)
                     /locus_tag="PLK08_01510"
     CDS             complement(9965..11065)
                     /locus_tag="PLK08_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPS52203.1"
                     /translation="MAGLAVKPKKDCKYDIVSLGEVMIRLDPGDNRIHTTRQFKVWEG
                     GGEYNVARGLKRCFRKDAAVVTAIADNAVGRLLEDLIYQGGVDMEYVKWFPYDGIGRD
                     VRVGLNFTERGFGVRAATGCSDRANSAASQLKKGDIDWEKIFGCDGARWFHTGGIYAA
                     LSQTTPDVIIEAVTTAKKYGTIVSYDLNYRPSLWKSIGGQEKARQVNRAIAPYIDVMI
                     GNEEDFSAALGFEVEGLDKDCSKLDPANFKKMIAKAVGMFPNFSIVATTLRNAKTASI
                     NDWGAIVYGDGQFFEATFRPDAEIYDRVGGGDSFASGLIYGLMEGKTPQQAVNYGAAH
                     GLLAMTTPGDTTTASLKEVERIMQSGTARVDR"
BASE COUNT         2845 a         3146 c         2744 g         2347 t
ORIGIN      
        1 cttcagcacc gagatgctcc tgtgtaatcg acggcatgtc agccggcaca gtcgattccg
       61 gctgaacggc cgcaatacga tttaaaaatt catcggctaa cgcacggtaa tcccttgcgc
      121 cgttgcattg cgcgtcgtat tcaaaaatgc tctggccgaa agacggacac tccgcaagtt
      181 tgatgttgcg ccgtatggcg gtgtgataca cgcatgcgtc agcccagggc ttgccggaag
      241 tcctggtcga cttgaaaaat ttcataacgt ccgtaaccac gtcgcgagcc agccgcgtga
      301 cctgttcgaa catgcagaaa accacgcccg caacacgcag tttcggattt atccgctgat
      361 tcacaagttc tatcgttcgc aacagtttgc tcaaaccctg cagggcgagg aactgcggct
      421 gaagcgggat tatcacatca tctgtagcag ccagcgcgtt cagcgtcaat aatccaagcg
      481 acggcggaca gtctatcatc atgaaatcca cgtgcgactt catggcgtct atcgcatttc
      541 gaagaatcat gttgcgattg ggacggctaa ccagttccgc cgtagccgcc gccaggtcgg
      601 tcgtggcagg cacgacccag agattgtcgc gaactttcac cattgcctcg tccatggtcg
      661 cctgttcgca gagcacgtca taaatcgtgg cggtgtcgtc ggcaggttca aaaccgaaat
      721 gcagcgtaag gtgactttgc gggtcgaggt ctatgaccgc aaccttccgg cccgcagcag
      781 ccatcgccgc cgccaggttc gcaaccgtgg tggtcttgcc gaccccacct ttctgattta
      841 tgatggatat tactttcaca gagggtatta taaaaattat tgcgaattat gccactgatt
      901 aagcagcttt tttgcattgt cacacagcgg cgattcaggg taatttttca gcagcttttc
      961 aagcagctcc gccgcccgtt gcggctgaga gtttttttgg gcatattcag caccagccaa
     1021 cagaagttgc ggtgcatact gactttgcgg gttgacgttc acaagcctag ccgcacagtt
     1081 caccgccctg acgtaccgcc cctgctcacc gaacattcgc actcgcagga gcgtaagata
     1141 ccctgtcagc ctgtcagtgg gaatcgcatc ttcccacttc tcaagcacgt cagccagcag
     1201 ttcgtattcg ccgcgatgca gataatcctc agcctgcctc gcgaaatttc ctctcgccgt
     1261 aaccggattt ttcttaacca ggtcacccga cagcccagcg cgcatataat acttattagc
     1321 ctgcgaataa tcgccggcag ccatacatgc atcgccggcg gcaatgattt cccgacggct
     1381 cattttctgc ggcacgtcat cgtcctgccg gccgaaccgg taacatcgaa ctgcggcgtc
     1441 aacatcgtca agctcgtaca gatagatatc gccggcccga acgtacaggg caacagccgc
     1501 atcgccatgc tttctgccgg cagctaattt ttccaatgcc tgagccgcac ggcctgcctg
     1561 cccggccaga ataagcatat cgcaataagt ccccaacacg ctttcaagct cgcgggcagt
     1621 aatgttcggg cgatttacga acatttcgca aagtcgcatc atgccgtccc aattcccggc
     1681 cattttaaaa agctcgacag ccgtcatcaa atccgtcgcg gaaaccgccg ataaatcata
     1741 atcgccgact atctttccat aaccggcagg ctcttcagcc acatctcgca tgaccatcgg
     1801 ccaatcgcga gaaacgtaat atcgctgcgt aatgacatca aaaccctttt tggcggtcac
     1861 agcgtacacg ccgtcatgca ggtaaacgtg cgaaactttt cggcctgcgc cggtctggcc
     1921 gtcgccgaag tcccactgcc agccttcgcc gtccgcatcg cttccgacga cctcaaaaac
     1981 gcgccgctga atatggcgat tgtcaacaac cgcctcgcct ctttcgttaa cgcggatatc
     2041 cagagtgcct ggcaaaccgt atcgctcagg caggccggcg gttgctgaat atatgcgagt
     2101 gaaaaactgt gccggcatga cttcaaatcc cgattcgcca ggctgacgcc agtaaaccgc
     2161 catcgtcgga tagccccgcg gcgtaacctg caagacgtcc atcctgtgca agccggcagt
     2221 caagctgacg ttgcgacgta tcgtcggctg tcgaaccggg ccatgccagg ctggcgtctc
     2281 aagaagcagt ttggaatcaa tggacaaaaa cgacgcgtta ttgctcgaaa tgatgaacag
     2341 gtaactgccg ctttgcggtg cgacaaacca gccggtataa acatttatca ggctataatt
     2401 gcccgtaaaa ggatttatgg cggaaaatat ccgcggcaca aacccgcagc cgacgaaatt
     2461 tttgggattt ttcttcgatt tctccaaagt tatcgtcgcc tgcggatagg cgtcggcatt
     2521 gccgcccaca tatcgccagc tttccaacat cagcccgcgt tttatatcca aagaactttc
     2581 cgccacagcc gactggttgc cgtaatagac ataataatcc tttacgcccg aaacaggagc
     2641 gaatgccacc cgaatcaaat cgcccggccc gcagcaaagc actttatgtc gaacctgcac
     2701 attgtcagcc gtacatacgc ggacgtctcc gccgtcgtcg cgagttttac cgccggtatg
     2761 gaattcaaca acgccgacgt cgtcgccagg tagttgcgag gccgtaaatt cttcgtcaag
     2821 ggaaacattc acgcgcaacg gccacgcagt ctcccaccag gcctggccgg caaagcaaat
     2881 tcccgaataa aaaatcatca gccctattgt caaaacattt tttcgcatgg atgcatttta
     2941 tcggcccgac agccgtgaag aaactgtatt tttgactgct ttgaagttat cattacgcac
     3001 atgcttgaaa ctggcgtaaa aacatcgaaa tacgacggcg tgatattcga catggacggc
     3061 acgctggtgg agtccagcct ggacttcgac gtactgcggc gagagcttga aatgtcgccc
     3121 ggccgcggca tacttgaaca gataaacgaa atgccgccca gccgacaatc tactgcgcat
     3181 tccaaattgt tggagttcga acttgccggc gccagaaatg ctatcctcaa tgacggcgcc
     3241 gaagagattt taaacgaact gctcagccga cggataccca tagccctgct tacgcgaaac
     3301 acgcgacagt cgtttgaaat cgtctcgaaa aaatttccga ttatgaaaaa ttttgatatc
     3361 gtcatgtgca gggaagacgg cgtcatcaag cctgagccgg acggcattat actggcctgc
     3421 cgaaagatgg gcatcgtccc gcaaaaaacc ctctgcgtcg gcgattttga attcgacatg
     3481 ctcgccgccc gtgctgccgg cgcaataggc gtactgctga ccacacatcc gaactggaaa
     3541 aattatgctg acaaggcaga ttatattatt aacgacttgc ctgcactgct gaatattttt
     3601 gattccaggg attgacggcc atttgttaaa ttttctgaat tttttatctg aagacaaaaa
     3661 aatgaatctg ccactggcaa acatattgca taacaagatg cggagtctct tttgcgtaac
     3721 gggcataggt ttgggcgtat gcatgattat aacgctcgcc ggcctgtccc gcggcagcct
     3781 gtacgaaata gccgaccgca tgaactgcgt tgacgcggac gtaatcatcg tcccgcccgg
     3841 ccttgccgac aaaacaccca cactcagcgg aatcgccatc agcgaaaaag tctccgatat
     3901 tcttatgcag aaatatcccg acgtcatagc ccgctgtgcg gcagtctgca tcggctcgct
     3961 caagctcggc ggccaggacc agcgcgtcat gggcatagac ccgcgcgact gggacatgat
     4021 aacgaatcga gcagaactca gcggcggcag gctgttcgac ccggacaacc gtttttcggc
     4081 gtggctgacg gaacaactgt taaaaccggc agaagacgat gacgataacg gcgagctgct
     4141 gaatctttcg gacaggctgg catcgcacgg cgggctggag attgtgatag actcccgcct
     4201 ggcagccgcc ggcagataca ggctcggcca gatggttcac gccggcaatc acgactggaa
     4261 aatcgtcggc atagtgccag ccggcgtgcc gacgcgcgta cttacgccta tccgcaccgc
     4321 gcaatacctc ttcggcttcg gcgacattaa aaaatgcacg atgatattcg tgaagttcaa
     4381 acccggtatc gacgctgcgc ctctcgcggc acagataagc aagcagatga aactcgacgt
     4441 cctgccgatg tcgcagtacc gcacaatgct tatggacaga ttcggcgtaa tgttcagcta
     4501 catcgacgcc gtcaatgccg tcgcactgat aatcgcgttc ctgatagtta ccgtcaccct
     4561 ttacatgatg gtacttcagc agacccgcga aatcgcgata atgaaaagct gcggcgcagg
     4621 cggaacgttc atattgcggc aggtcatggg cgaggcgcta ctgctgacgc tggcgggcat
     4681 ggcagtcgga gtcggtatga gttttccggc ggctctggcg atatcgcatt tcaggccgct
     4741 gcttacagtg ataataacgc cgggctggat tttgacggca tctgcggcgg cgctcgtcgg
     4801 cgccgtcgtc agtgcgctgt atccggcatg gcgggcatcg aaagtggaca tgttggaagc
     4861 actcacatat gaatgaccct gaaaaacaca ttctcagcgc cgtgggcctt aataagagtt
     4921 tcgggcgggg cgaggctgca aatcatgttt tgcgcggcat agatatcggc gtaaatgcag
     4981 gcgaattcgt tgcagtcatg gggccgagcg gctgcggcaa gagtacgctg ctgcacattc
     5041 tgggtctgat gacatcggca gacagcggcg agatttttta caacggccaa gccgtcacgc
     5101 acagcgaatc atctagacgg aaactgcggc gaaaccacat aggcttcatc ttccagcgat
     5161 tcaatcttat cggcacgcta agcgcagccg acaatatccg catttccctc cgcattcgcg
     5221 gcctgaaaga cgacggcaga agcgacgaac ttctgaaact catgcacgta tcgcatgtcg
     5281 cgcgacgtcg gccgggccag atgtccatcg gcgaacagca gcgcgtcgcc gcggcccgcg
     5341 cgcttgcaca caagccggct atcctctttg ccgacgaacc gaccggcaac ctcgacagcg
     5401 aaaacggcct ggcgctgctg gaacttttca aaaaggtcaa cacggaactc ggccagacaa
     5461 tagtgatgat aacccattcc aaccaggcag cccaatgggc acacagaata atacgcatga
     5521 aagacggaaa aatagatgga tgacgtaact gcaaaatcgg aatattccca catccgcgtg
     5581 cgatggtggt ttgccgcatt tgcagtcgcc ttcgcaatag gcgtttgctg gctggcgtgg
     5641 ctgataaaaa cgcagcagtt gcaattcagt acatggggcg aattcaaaag cgccgttatt
     5701 acggcatcgc cactgctaaa gctgctggtg ctgggcctgt atctgtccat ctgctgcacg
     5761 tttctgccgc tgaacacgag ctggataata tccgcggctg ccatgcaggg aactgccgtc
     5821 gccggcaaca tgtggcagac ggttctgctg gtcagcctcg tcggagcagc cgcttcaacg
     5881 gtggcaaacc tcaacgatta tcatcttttc acggtcatga tgcgatatca tcacatcgcg
     5941 aaaatccgtc acacaaggac ttacgccgtc gccgtaaaat ggttcgaacg ctcgccattc
     6001 gcactgctgc tgcttttcaa cgtcctgccg atacccgtag acgtagttcg tccgctagca
     6061 gcagcacata aataccggcg tacaaaattc gcagccgcca attttatagg ccgattcgct
     6121 agatacgcag taatcgcggt tataacctac atgctcggca gacagggctg gatagcgact
     6181 ctcgccttac tggcggcggc agtgatactc gctctgccga aactcgcagg aaaaatcctg
     6241 gccggacgca aacacaattt accggagtca acagatgaaa aaatatcagc ttgagtgtat
     6301 ggttcttgcc gccgtcgtat ctgccgtcgc atcttgtcct gcacagcagt gtcaaccgac
     6361 ttgttcaccg caagaggccg tatcgccgca aaccaaagcc atcctgaacc ggctgcaagc
     6421 cgccggcgac agatacacca cactcactgc cgacgttaac tatgtcgtcg aaatgcgtat
     6481 gaccggcgaa actgaaacac gcgacggatg ggtggcgttt cagaaaaaca ctgaaacgac
     6541 tccggatatg ttccgtattc attttgacac gctgaaactc gaccagggca aaaaaacggc
     6601 atcgaagctc gattatattt ttgacggcca atggctgacc gtggccaagc acaacatcaa
     6661 gtcaatgacg cgattccagg tagcaaagcc aggcgaaaaa atcgaggctc tgcgaatcgg
     6721 caagggccct ttccccatac cattcggcca aaaaactgac gacatgataa aatgcctcca
     6781 gataaccacc cgcgacgcag aaccggacga tccggcaaat accgattacc tcaaactcat
     6841 ccctcgaaaa aattatgaag accagctaaa tttcaaaaaa atggaaatat ggattgaccg
     6901 cgaaaccaat ctgccggtaa aagtagtcgt gcgcgacagg aacaaaaacg tcaccactgc
     6961 cgaattttcc aacataaaga cacagtcagc aatcgacccg tcgctatttc agatggaaaa
     7021 gccggcaggc tgggatttga cgattgaacg tattaaacaa aactgaaccg tggggagacc
     7081 gatgagcgaa aaattttaca taatcgacgg ccactcgcag atatttcggg catattacgc
     7141 accattccgc gacctgacca gtccagccgg cgaacccacg cgggcgacat acgtattcac
     7201 aaacatgctc ctgaaattca tcgccgacaa aaaacccgac tatctcgcca tggccatcga
     7261 cggcccgcat gaaaaactaa accgcacaaa aatctttaag gattacaagg ccacccgcaa
     7321 acctacgcca gaagatttca agccgcagga aaaacgcata ttgcaaatcg tcaaaatact
     7381 cggcatcccg attctggacg ccgaaggcta tgaagccgac gacatcctcg ccacgctggc
     7441 cgacaggtac gcatccgaaa atgtcgacgt aatcctcgtc agccgcgaca aagacctcga
     7501 ccagctcatc aacgaccacg tcatgcttta cgacccgatg aaagatgaaa ctctcgacgc
     7561 cgcctcaatc gagcagagca aaggttaccc cccgtcatta gccgtcgaga ttcagacgct
     7621 tatgggcgat aatatcgaca atattcctgg cgtgcctggc gtaggcccaa aaacagcggt
     7681 caaactcgtc aggcagtacg gctcagccga tgcggtgctt gcggcggctg atgagcagac
     7741 gccaaaactc agggacagcc tcaaaactct cggcagtgac cggctggcga cttcccgcaa
     7801 actcgtgacc ctcgataaac atgtgccgat gaaaaccgac ctgccggaat tccgcttcag
     7861 ccagccggac atgcaaaatg cgtcaaaaat tttcatggag ctgggcttta caaaactcgt
     7921 cgaaaaatac gcggctgaaa aaccggcacc aaaatcggcg gcaaaaaatg acccgtttat
     7981 cggcggactt tttgcgtcgc tggaacagtc agccgcaagt tcaccggttg cggctgcgac
     8041 aactgatatt aaaaaatcac agcctgcaat gccggatgga tttaccaccg cagccaattt
     8101 tgattaccag ttaatcgaca cgcaggacgg tctcgacagg ctggccgcat cgctgcgcga
     8161 cgtaaagcgg ttagccgtcg ataccgaaac gaccggcata aaagcgatga actgttcgct
     8221 ggttggcata tcgctggcat ggaaggctgg acacggagtt tatattccag tcatgggccc
     8281 gttgggcgcc acggtacttg acatcgggaa aatacgcgac gcaatcgggc ctgcgatggc
     8341 tgacgcgacg gtggaaaaaa tcggccacaa tctgaaatac gatttgatag tgctggaaaa
     8401 caacggcttt acgtttgccg gaaaaatgtt cgacacaatg atagccgccc acgtgattga
     8461 ctcgcaacac gcaagcctga aaatggatta cctgtcgctt gagtacctgc atcaccgttg
     8521 cataccgata accgacctca tcggcagcgg ccgcaaccag accaccatga acaccgttcc
     8581 cacagatacc gtcgccgtat atgctgcgga agatgccgat gtaacactgc ggctggctga
     8641 cgcgatggaa aaaattctgg acgaaatggc cctgcggaat ctttttgaaa atctggaaat
     8701 gccgctcatg cccatcctga cagaaatgga acgtaacggc ataactgttg acccggccag
     8761 gctcaaggcg atgcagtccg agttttccat gaaagccgac gtactcaggg acaggattat
     8821 ccgactcgca ggcgtcaatt tcaatctcga atccccaaaa cagctggctg agattctttt
     8881 tgtaaagatg ggatatcccg caatcaaaaa aacacgcacc ggcatgagta ccgactcgga
     8941 agtcctggaa caactcgcgt cggaacacga ctgcgaaatc gccgcactgc tgctggagta
     9001 tcgcaaactc acgaaactta taagcacata cctcgtctca ctggtcgact gtatcaatcc
     9061 gcgtaccggc agagtgcata cgtcgttcca ccaggcatcc gtcatcaccg gccgattatc
     9121 cagcagtgac cctaatttgc aaaacattcc ggtacacgac gaaggccggc agattcgttc
     9181 ggcattcatc gccaagcacg gctgcaaact gattagcgca gattattcgc aggtcgaact
     9241 gcgaatgttg gcgcattttt gcggcgacga aacactctgc cgcgcattcg cagatgacct
     9301 ggatattcac cgcatagtgg cgtcggaagt tttcggcgtg gatgtggatg acgtcacgcc
     9361 agccatgcga agccgcgcca aaaccgtgaa tttcggcata atatacggcc agacgggctt
     9421 cggcctggca agaacgctcg gcatcggcag aaccgaagcg tcggaattca tacgcaaata
     9481 taaacagcgt ttcccgaaaa ttgaagtgtt tatggctgaa tgcgtcgaac aggcaagaaa
     9541 caagggctat gttgaaacca tatccggccg ccgacgccgg atacctgaca tcgattcccg
     9601 caaccagcag aaacgtaatc tggcagaaag aaccgccata aacaccgtcg tgcagggctc
     9661 agccgcagac ctgataaagc aggccatgat aaacctcgac cgtacacttg aaaaacagaa
     9721 cagcccggcg aaaatgctgt tacagattca cgatgaactc atttttgaaa caccggctga
     9781 atcggcggaa tccactgcaa aagtaatcgc ccatgaaatg tcgacagcca tcaaactcaa
     9841 cgtgccactg aaagtggact gcaacatcgg cgacaactgg ctggaagcca agtagaatcc
     9901 acctgctgaa agcataaacg tcgagaataa aactgctatt gcatgaacac acaaatccga
     9961 tgttttatct gtcgactctt gctgttccgc tttgcatgat tctttcgact tctttaaggc
    10021 ttgccgtagt tgtatcgccg ggggtcgtca ttgccagcaa tccatgtgcg gcgccgtaat
    10081 tgacggcttg ctgtggggtt ttgccttcca taaggccata aatcaatccg gaagcaaaac
    10141 tgtcgccacc gccgacacgg tcgtaaattt cagcatcagg ccggaatgtc gcctcaaaaa
    10201 actggccatc gccatacact atagcacccc agtcatttat tgacgccgtc ttcgcattgc
    10261 gaagcgtggt ggcgactatt gaaaaattag gaaacatccc gacggctttt gctatcatct
    10321 ttttgaagtt ggccgggtca agttttgaac aatccttatc caggccttca acttcgaatc
    10381 caagcgcggc ggaaaaatcc tcttcattgc ctatcatcac gtcaatatac ggtgcgattg
    10441 cacggttgac ctgccgtgcc ttttcctgtc cgccgataga tttccaaaga gacggccgat
    10501 aattcaggtc gtaagacaca atcgtgccat actttttcgc cgtcgtgaca gcctcgatta
    10561 tgacatccgg cgtcgtctgg gaaagcgccg cataaatgcc gccggtatga aaccacctcg
    10621 ccccgtcaca gccgaaaatt ttctcccagt caatgtcgcc ctttttaagt tgcgaggcgg
    10681 cactgttggc ccggtcggaa cagccggtcg ccgctctaac gccaaaacct ctctccgtga
    10741 aattcaggcc gacgcggacg tcgcggccta tgccgtcata cggaaaccat tttacgtatt
    10801 ccatatcgac gccgccctgg taaatcaggt cttcgagcag ccggccaacg gcattgtcgg
    10861 cgatggctgt aactactgcg gcatctttgc ggaaacagcg tttaaggcct ctggcgacgt
    10921 tgtattctcc gccgccctcc cataccttga actggcgagt cgtgtgaatg cgattatcgc
    10981 cgggatccag ccttatcatg acttcgccga gagaaactat atcgtatttg caatcctttt
    11041 tgggtttaac cgccaagcca gccatcattc acctcttatc tg
//
LOCUS       DAONFI010000025        11057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6668_length_11057_cov_5.044901, whole genome shotgun sequence.
ACCESSION   DAONFI010000025 DAONFI010000000
VERSION     DAONFI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_11057_cov_5.044901"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLK08_01515"
     CDS             <1..1137
                     /locus_tag="PLK08_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPS52204.1"
                     /translation="YGEAFVNDAQRNIEKAIKYQSLRENLEQQLGVARKKTNNETQIE
                     EIEEKLDKVLEVTRMESPEYRVILGQGIQGLSARTEKGDTIRFMLPGHRIILYVLPLG
                     KNLSMTDFAPNTHKFTIIDDNKADVSSIDSKMIYIPFETLQKLNNMDVEHDPETRQMT
                     VPARCSQIHIKVRGDTNERRLEAIAAKIEDVWKNFSARYADKPFGLQPAPNGLMIQTW
                     RQRQVNVVAPIESQRTLVIIITVIMSFVAIALIFVILYTIVVQKTREMGLLKAIGAGS
                     MGVAGLFLRYGAAIAVIGSIAGIIGGAYFVRHINAVQDWVDGTFGFRVWSRETFMFEF
                     IPDKVDWTAACWIFVGAIFAGLLGALLPAVRAGRMQPIEALRYE"
     gene            1685..1939
                     /locus_tag="PLK08_01520"
     CDS             1685..1939
                     /locus_tag="PLK08_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52205.1"
                     /translation="MKHFVDTLSRAERMLVILRHELYEDSWNEMLADMKARLAGEPYV
                     FKLADRIEDDMDRIEKLRNFEARWAVDLADYISLFRTDDA"
     gene            1972..3204
                     /locus_tag="PLK08_01525"
     CDS             1972..3204
                     /locus_tag="PLK08_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPS52206.1"
                     /translation="MLKMWKIRSFALLLLAAMFVVGCEPQKPQAEAVGLDDAAGNAAD
                     KSLGKLDLRILWNRDMPMADNDSVVNFYCFGDTLVLVSEYNGVYAVDASNGNPKWAVR
                     LTEAVETVYQPSRVKISLTPQPVGQGRQDVDVSKDLKNYDAILINSTTRLMLVDNATG
                     NVLRDIPLTFIAAGAAACNGRQAVVMSSFGAVRQIDLLAGVCGFNKIIEQDSSVPMVF
                     YEGLYFIGSQQGKMLCYTVKNNIEKNWEFDVQGPINTPFVVNDMGLFVAGGDKRIYGL
                     SLQKGERLWLPVRIYGSFAGPMAAGENLLFQYAIGSGLSAISMDSGKLAWTKPEGLVV
                     LANFDDRVYLLTNSRKLMVVREADGESLGTASLKPFTKFAVNTAHRGIFAATAEGKII
                     CISPDNVEDLTVGDVAGN"
     gene            3240..4868
                     /gene="purH"
                     /locus_tag="PLK08_01530"
     CDS             3240..4868
                     /gene="purH"
                     /locus_tag="PLK08_01530"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPS52207.1"
                     /translation="MSNVRIKRALISVFDKTGIEDFARALVDMGVEIISSGGTAKALE
                     AVGIKVRRVQEWTGFPEMLGHRVVTLHPKVHGAILALRDDPSHQADMKKYGIEPLDLV
                     CVSLYPFEKAVAKPGCTLADAVEMIDIGGPTMVRAAAKNHRYGTVVTDPSQYEELLGQ
                     MKQNGGEVSFDLRQKFALQAFSLTCAYDSAISGYLAEQLGQEFPDRFSVSFRKSSELR
                     YGENPHQKAAFYVSGEAEKEPCMGRAKILGGKAISYNNYFDANGALELVKEFTSPAVA
                     IIKHANPAGCAMDDDLVEAYRKAYFGDVNAAMGGIVAVNRPVTCELATAITESYQRWG
                     KAAGAGGFFAEIVIAPSFEPGAAEIIRNRQGWGPNVRMLEVGEFDGRRNKEKFLKYCV
                     GGMLVQERDLLGLNLDDWKVVTKRQPSDREMDDLKFAWLVCKHVKSNAIVLAKDRTLL
                     GAGAGQMSRVNSAFLAGQIAKGYGGGNAVDMNGCVLASDAFFPFPDSLDWARDAGATA
                     VIEPGGAKKDQDVIDYADKLGLAMIFTGARHFNH"
     gene            4962..5564
                     /locus_tag="PLK08_01535"
     CDS             4962..5564
                     /locus_tag="PLK08_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPS52208.1"
                     /translation="MNDSLAASTREYVQRREMTLEERLAEERILFLWGEIGPQSAGGV
                     IMHMLELQSKNPTREIHLYINSPGGSVDDTLAIYDTMQFLTCPVATYCVGQAASGAAL
                     ILASGTKGKRFALPHSKVMIHQPYGGVTGQVSDVKIQAEEILKAKKLLNEILSRHTGK
                     DVETVRQATERDRFLTPAEAVEFGLVDEIIESHSQNKKDK"
     gene            5561..6193
                     /locus_tag="PLK08_01540"
     CDS             5561..6193
                     /locus_tag="PLK08_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPS52209.1"
                     /translation="MIMASNHLVPIVVEQTGRGERSYDIFSRLLKDRIVFIGGPVDDN
                     LANLVVAQLLFLSNEDPHSDIHIYINSPGGSISAGMAMLDTMQFVRCDVATYCIGMAA
                     SMGAVLLAAGTKGKRFSLPNSRVMLHQPLLSGVLEGPATDLDIEAQELLRIRNKLYEF
                     LADATGKNIETIEHDCDRNKWLDAIEAVEYGMIDKVLDRMPETKKNENEA"
     gene            6183..6800
                     /locus_tag="PLK08_01545"
     CDS             6183..6800
                     /locus_tag="PLK08_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52210.1"
                     /translation="MKRNVQIAFLGLFCCLLAMAGCEDPMLKHEREMQFRQAELERKN
                     ALQADEIQKLQANEKELQTQVDTLKKIGDKRPDEFVKITRMRISSYTGGVDRDGKPGH
                     DAVKVMFTPEDSAGDPVKTAGTVKIELFNLAAAKPADKLLGTFNYTPGEAVKSWAGGM
                     FTNHYSFILPLPKTFKGGELTVRVEFTDYFSGKTFSGQKVCKIRK"
     gene            complement(6832..7863)
                     /locus_tag="PLK08_01550"
     CDS             complement(6832..7863)
                     /locus_tag="PLK08_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HPS52211.1"
                     /translation="MNKIWNIYDGRVLVRQTPWSIRIGVPAVLLTLFCTGTCVTYFYI
                     HPDTTVTESIYMTVITIATLGSRDAGRGGEWWTIITILGGLTLITILGAQITAFIVEN
                     RMLGIIGRQKVKKKIEALEGHVAVCGFGRTGQQVAMQLRDSGKTILVVENDPQRVSVA
                     LSCDFLVIQGDAQEEEIIAQMRLPLASTLVTCLANDAANLFLTLSARQENHELFIIAR
                     VMQESSMSKLRRAGANRVICPQLVGSNRIVDLVLRPEMVDFLEMTHSGVDLEMDRYEI
                     TPQSPLADKTLAEIALPHHVGAQVVAVRHKNGHINYHPTHDLKLIVGDVIILVGKEGI
                     NRAMEQFKM"
     gene            8081..8764
                     /gene="ispD"
                     /locus_tag="PLK08_01555"
     CDS             8081..8764
                     /gene="ispD"
                     /locus_tag="PLK08_01555"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPS52212.1"
                     /translation="MAKVSVIIPGAGRGLRFGGSMNKIFELLGDMPLFLRTIEAFIKR
                     PDVLEVIFVCSMDDMEYVAQHYGWRLEKLGVKLVAGGSTRTQSVKNALDAVSDEAELV
                     AIHDAARPCIAQSWIDAVFSQASMCGAALLAVPIHGTVKRTDGRGIVTQTLPRDEYAN
                     LFEAQTPQVFAREIIMKVYENPAEATDDCALVEQAGYPVSVVHGDLRNIKVTRREDMI
                     FARQVISTL"
     gene            8794..9927
                     /locus_tag="PLK08_01560"
     CDS             8794..9927
                     /locus_tag="PLK08_01560"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS52213.1"
                     /translation="MGFSDRKVLLITASVGSGHNQAARSISEGLAEAGCDSMTIDVMS
                     YVPAWFRWYYSGGYSLAVSRLPFLYGVGFRLNDMPDLPHRNISEKIRIAFESAAASRL
                     VRAVFDYKPDIIVNTHFLAPPILARLGYDNQFIVVTDIQAHRWWYSEGIDRWFVQQPQ
                     SANRLIEKWNVPQGKITVSGMPVRMAFDTSRSDGDDLRRELRLPAGRPVVLLSGGTVF
                     TCGPVPAIARGIASICPNACVVVLAGRNKKLLARLGSMAESRDGRVVPMGFTDRVSDL
                     MDAASLMVTKSGGMICAECIAKSLPAVFMPPVPGQEAQNAKYLAECGAARVAEKFSDI
                     GGIVAEMLADGDGLANMQKAAGGLKRPARKIIVEKIIERISNH"
     gene            10026..>11057
                     /locus_tag="PLK08_01565"
     CDS             10026..>11057
                     /locus_tag="PLK08_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPS52214.1"
                     /translation="MRYAIIMAGGSGKRLWPCSRTNRPKQLIPMVEGKSLLEIAVERL
                     LGLFEPEKIFIITNRDYADKVCAAIDKLPAENIIGEPSGRDTANAVALAAAIIEGRDP
                     HATMAIFTADHIIRPESCFIEAVELAMKTAEKNPDSLVTFGIRPNWPHTGLGYIECGD
                     TVAPGVHKVESFREKPDHPTARKYVESGRYFWNSGMFVWKVSAIQAALREFLPASAAK
                     FDAVTRAVRKSEDVGPILDKVYPELQKISIDYAVMEKTQGVLMTELNCEWIDLGSWPA
                     LENVLDQDDKGNTILAAHHVVMDSFNNIIVSEDESHIIAMLGMDDCIVVQSGNATLVC
                     RRSDSQRLKE"
BASE COUNT         2633 a         2759 c         3118 g         2547 t
ORIGIN      
        1 tacggcgaag ccttcgtaaa cgacgcccaa cgcaatattg aaaaggctat caagtatcag
       61 tcacttcgcg aaaatctgga acagcagctt ggcgtcgccc gtaaaaaaac aaacaacgaa
      121 acgcagatag aggaaatcga ggaaaaactg gacaaggtgc tcgaagtgac gcggatggaa
      181 tcgcctgagt atcgtgtgat tctcggccag ggcattcagg gcctcagcgc ccgtaccgaa
      241 aaaggcgata ccatacgctt catgctgccc ggccatcgaa ttattcttta cgtcctgccg
      301 ctggggaaaa atctcagcat gacggatttt gcgccgaata cgcacaagtt taccataatc
      361 gacgacaaca aagccgacgt ctccagcatc gactccaaaa tgatttacat cccgttcgag
      421 acgcttcaga aactgaataa catggatgtg gagcatgacc ctgaaacacg gcagatgacc
      481 gtgcctgcac gttgcagcca gatacacata aaggtacgcg gcgatacgaa cgaacggcgg
      541 ctggaagcta tcgccgccaa aattgaagac gtctggaaaa atttcagcgc ccgatatgcc
      601 gacaaaccgt ttggccttca gccagcgccg aacggcctca tgattcagac gtggcgacaa
      661 cgccaggtaa atgtagttgc gcctatagag tcgcaacgta cgctggtcat catcataacg
      721 gtgataatgt cgtttgtcgc cattgcactc atattcgtga ttctttatac gatagtcgtg
      781 caaaagacgc gggagatggg tctgctcaag gcgataggcg caggcagcat gggcgtagcc
      841 ggcctttttc tgcgatacgg cgcggctata gctgtaatcg gctcgatagc cggcattata
      901 ggcggcgcct attttgtgcg acatataaat gccgttcagg attgggtgga cggtacgttc
      961 ggctttcgcg tatggtcgcg ggaaacgttc atgttcgagt tcattccgga taaagttgac
     1021 tggacggctg cgtgctggat attcgtaggt gcgatattcg ccggccttct cggcgcgctg
     1081 ctacccgccg ttcgagccgg cagaatgcaa ccgatagaag ccttgcgata cgaatgattc
     1141 catggcaatt ttcgtatgtc tgccggagaa tccctctaaa actgacggta tttttttgac
     1201 cggaacgaaa aatctgatta tgcgataatg attattgagc ggtagggctg acagccttac
     1261 cgttcggaag gctagagcat attaaacttg actttgcgct tgttgctgtt tgcggcgttg
     1321 gctggctgcg tcaggctgca tcggaaacca tataaggttg cctgcttcgc cttccttgcc
     1381 agcctcgtcc tcaacgacgc aacaaggcgc aatttcaaat ttaacatgct ctaatatccg
     1441 ctaaaatttt tgataaccgc tctaatcgga aaaagttgag agaacagttg caatctgcct
     1501 gccagcctgt tgaaagctgt tgataactcg acaaatccat ctttcatttc atgttatgtg
     1561 gggaaatggt tgaaaatacg tcagtcttat cgtaaatttg taaagaattt tttaagacag
     1621 attcgtataa aatttttaca gaggtgcttg agtgtccggc caaagtgaat atgcccgaca
     1681 gcgagtgaag cattttgtag atacgctgag cagggcagag cgaatgctgg tgatactccg
     1741 ccacgagctt tacgaggata gctggaatga aatgctcgcc gacatgaaag cccgtttagc
     1801 cggcgagccg tacgttttta aactggctga ccgtatcgag gatgatatgg accgtatcga
     1861 gaaacttcgc aatttcgaag cccgctgggc tgttgatctt gccgattata tctcgctttt
     1921 cagaaccgat gatgcgtaaa ccgtaatgtc gtatttatca aggagatgtc catgctgaaa
     1981 atgtggaaaa ttcggtcgtt tgcgttattg ttgttggctg cgatgtttgt tgtcggttgt
     2041 gagccgcaaa agccgcaggc tgaggctgtc ggccttgatg atgccgccgg caatgccgct
     2101 gacaagtcgc tcggcaaact ggatttgcga atcctctgga atcgtgatat gccgatggct
     2161 gacaatgatt ccgtagtcaa tttttattgt ttcggcgata cgcttgtgct tgtgagcgag
     2221 tataacggcg tatatgccgt ggatgctagc aacggcaatc cgaagtgggc agtgcggctg
     2281 acggaagcgg ttgagaccgt gtatcagccg tcccgcgtga aaatatcgct tacgccccaa
     2341 cccgtcggcc agggccgtca ggatgtcgac gtatccaaag atttgaaaaa ttatgatgcg
     2401 atattaatca actcgacgac caggctgatg cttgtggaca atgccaccgg caatgttctt
     2461 cgtgacattc cgttgacctt catcgccgcc ggcgcggcag cctgtaacgg ccggcaggct
     2521 gtagtgatgt cctcgttcgg cgcggttcgc cagatagatt tgctggctgg cgtgtgcgga
     2581 ttcaacaaaa ttatcgaaca ggattcatct gtgccgatgg tgttttatga aggcctttat
     2641 ttcatcggct ctcagcaggg caagatgttg tgctataccg taaagaacaa catagagaaa
     2701 aactgggaat tcgatgttca ggggccgata aatacgccgt tcgtagtcaa tgacatgggc
     2761 ctgttcgtgg ctggcggcga caaacgcata tatggccttt cgcttcagaa gggcgagagg
     2821 ctctggctgc cggtgcggat ttacggcagt tttgccgggc cgatggcggc gggtgaaaat
     2881 ctgcttttcc agtatgccat aggttccgga ttgtctgcga tttctatgga ttccggcaaa
     2941 cttgcctgga cgaaaccgga gggccttgtc gtgctggcta atttcgacga cagggtttac
     3001 ctgctgacga attcccgcaa attgatggtt gtccgcgaag ccgacggcga gtcgctcggc
     3061 acggccagcc tcaaaccgtt tacgaagttt gccgtcaata ccgcccatcg cggcattttc
     3121 gcagccacgg cggaaggtaa gattatctgc atctcgccgg acaatgtcga agacctcacg
     3181 gttggcgacg tggctggcaa ttgattttta cgatttattc ccagggagta cgataaaaaa
     3241 tgagcaacgt caggattaaa cgtgcgctga taagcgtttt tgacaagacg ggcatagagg
     3301 attttgcccg tgcgctggtt gatatgggtg ttgagataat ttcatcaggc ggcacagcca
     3361 aggcgctgga ggctgtcggc ataaaggtac gccgcgttca ggaatggaca ggctttccgg
     3421 aaatgctggg ccatcgtgta gtgacgctgc atccgaaagt tcacggcgca attctggcgt
     3481 tgagggatga tccgtcgcat caggcggaca tgaaaaaata cggcattgag ccgctcgacc
     3541 ttgtatgcgt gtcgctgtat ccgtttgaaa aagccgtcgc caaacccggc tgcacgctgg
     3601 ctgacgctgt cgaaatgatt gacatcggcg ggccgacgat ggttcgcgct gcagctaaaa
     3661 atcacagata cggcaccgtc gtgaccgacc cgtcgcaata cgaggaactg cttggccaga
     3721 tgaagcagaa cggcggagaa gtttcatttg atttacgcca aaaattcgcg ttgcaggcgt
     3781 tttcgcttac gtgtgcgtac gacagtgcca taagcggtta ccttgcggaa cagctcggcc
     3841 aggaatttcc cgaccggttc agcgtgtcgt tcagaaaatc ttccgaactg cgttatggcg
     3901 aaaatccgca tcagaaggct gcgttttacg tttccggcga agccgaaaaa gagccttgca
     3961 tgggccgtgc gaagattctt ggcggcaagg ctataagcta caacaactat tttgatgcca
     4021 acggagcttt ggagctggtc aaggaattca cgtcgccggc agtggcgatt atcaagcacg
     4081 ctaacccagc cggctgtgca atggacgacg acctcgttga agcttatcgc aaagcctact
     4141 tcggtgacgt caacgccgca atgggcggca tcgtcgctgt aaacaggcct gtgacttgtg
     4201 aacttgccac ggcgataacc gagtcatacc agcgctgggg caaagccgcc ggcgcaggcg
     4261 gttttttcgc cgaaatagta atcgctccga gttttgaacc tggtgcggca gaaattattc
     4321 gtaatcgcca gggttggggg ccgaacgtcc gcatgttgga ggtcggcgaa tttgacggca
     4381 gacgcaataa ggaaaaattc ctcaaatatt gcgtcggcgg catgctggtg caggaacgcg
     4441 acctgttggg gctgaacctc gacgactgga aagtcgtcac taagcgtcag ccgtccgacc
     4501 gcgaaatgga tgacctcaaa ttcgcctggc tggtctgcaa acacgtcaag agcaacgcca
     4561 tagttctggc taaggacagg acgctgctcg gcgccggtgc cggccagatg tctcgcgtaa
     4621 attcagcctt tcttgccggc cagatagcta aaggttacgg cggcggcaat gcagtcgaca
     4681 tgaacggctg cgttctggcc agtgatgcgt ttttcccgtt tcctgactcg ctcgactggg
     4741 ccagggatgc cggcgctact gctgtcatcg agccgggcgg cgcaaagaag gaccaggacg
     4801 tcatcgatta cgccgacaag ctcggacttg ccatgatttt caccggcgct cgccatttca
     4861 atcattgagc ggttcgtgta taatgtcatc ctgctttgac gcacggcgag gcttacgccg
     4921 cggaatgacg aaaatacgag acggaattta agggatatca tatgaacgac agtttagccg
     4981 caagtacaag agaatatgtt caacgacgcg agatgactct cgaagaacgg ctggccgagg
     5041 agcgcatact tttcctctgg ggcgaaatcg gcccccagtc agccggcgga gtcataatgc
     5101 atatgcttga attgcagtcc aaaaatccca cgcgggaaat acatctgtat atcaattcgc
     5161 ctggcggttc ggttgacgac acgctggcga tatacgacac gatgcagttt ctgacctgtc
     5221 cggtggctac gtattgcgta gggcaggctg ccagcggagc tgctctgata ttagccagcg
     5281 gcacaaaagg caaacgtttc gcactgccgc acagcaaggt aatgatacat cagccttacg
     5341 gcggcgtgac cggccaggtg tcggacgtaa aaatccaggc ggaggaaatc ctcaaagcca
     5401 agaaactgct taacgaaatt ctgtcccgac acaccggcaa ggacgttgag acagtccggc
     5461 aggctacgga gcgggacaga tttcttacgc cggcggaggc tgtcgagttc ggcctggttg
     5521 acgaaatcat cgaaagccac tcgcagaaca aaaaggataa atgattatgg cttcaaacca
     5581 tctggtacct atagtcgtcg aacagacggg ccgcggcgaa cgcagctacg acatcttcag
     5641 ccgcctgctc aaggacagga tagtctttat cggcgggccg gtcgacgaca acctggctaa
     5701 cctggttgtc gcgcagctgc tgttcctgtc caatgaagac ccgcacagcg atatccacat
     5761 ttatatcaac tcgccgggcg gcagcatctc agccggcatg gccatgctcg atacgatgca
     5821 gttcgtccgc tgcgatgtgg ctacgtattg cataggaatg gcagcctcga tgggtgcggt
     5881 tctgctggct gccggcacga agggcaaacg cttttcgctg ccgaattccc gcgtaatgct
     5941 gcatcagccg ctgctgtcgg gcgtgctgga agggccggcg accgacctgg atatagaggc
     6001 tcaggaattg ttgcgaatcc gcaacaaact ttacgaattt ctggctgatg cgaccggcaa
     6061 gaatatcgag acgatagagc acgactgcga ccgcaacaag tggctcgatg ccatcgaagc
     6121 cgtggaatac ggcatgatag acaaagtgct cgaccgtatg ccggagacga agaaaaacga
     6181 aaatgaagcg taatgtacaa attgcgtttt tagggctgtt ctgctgtctg cttgcgatgg
     6241 ccggctgcga agatcccatg ctcaagcatg agcgggagat gcagtttcgt caggcggaac
     6301 tggaacgcaa gaatgctctc caggctgacg agatacaaaa attgcaggct aacgaaaaag
     6361 agttgcagac tcaggtggac acgctgaaaa agataggcga taaacggccc gacgaattcg
     6421 tcaaaatcac ccgcatgaga atcagcagtt ataccggcgg tgtggacagg gacggaaaac
     6481 ccggccacga tgccgtcaaa gtgatgttca cgccggaaga ttccgccggc gatccggtca
     6541 aaaccgccgg tacggtcaaa atcgagttat tcaacctggc agccgcaaaa ccggccgaca
     6601 aactgctagg cacgttcaat tacacacctg gagaggctgt caaatcctgg gctggcggca
     6661 tgttcacgaa tcattattca ttcattctgc cgctgccgaa gacttttaaa ggcggcgagc
     6721 ttaccgtcag ggtggaattc acagactatt tttcaggcaa gacattttcc ggccagaaag
     6781 tctgcaaaat ccgtaaatag ttttttgtga atccgggaaa taaaggccgg cttacatttt
     6841 gaattgttcc atcgccctgt ttatgccttc ttttccgacg agtattatca catcgccgac
     6901 gattaatttc aaatcgtgcg tgggatgata gtttatatgg ccgtttttgt gccgtacggc
     6961 aacgacctgt gccccgacgt gatgcggaag tgcgatttca gccagcgttt tgtcggcgag
     7021 cgggctttga ggcgttattt cgtacctgtc catttccagg tctacgccgc tatgagtcat
     7081 ttccagaaaa tccaccatct ccggccgcaa tacgagatcc acgattctgt ttgagcccac
     7141 gagctgcgga catattacgc gattcgctcc tgcccgccgg agtttgctca tggaactttc
     7201 ctgcattacg cgtgcgatta tgaacaattc gtggttttcc tgtcttgccg aaagcgtcag
     7261 aaagagattc gccgcgtcgt tagccaggca tgtcaccagg gtcgatgcca gcggcagtcg
     7321 catctgtgcg attatttcct cttcctgcgc atcgccctgt atgaccagga aatcgcagga
     7381 cagggcgacg ctgacgcgtt gcgggtcgtt ttcgacgacg agaattgttt tgccggaatc
     7441 ccgcagctgc atggctacct gctggccggt acggccaaat ccgcatactg ccacatggcc
     7501 ttcgagtgct tcgattttct tcttcacttt ctgtctcccg ataatgccca gcattctgtt
     7561 ctcgacgatg aatgcggtta tctgtgcgcc cagaatggta atcagcgtaa ggccgcccag
     7621 tatcgttatg attgtccacc actcgccgcc gcggcccgca tcgcgagaac cgagcgtggc
     7681 gatggtgatg acggtcatat aaatggattc ggttacggtg gtgtcgggat ggatgtaaaa
     7741 ataagtcacg caagtgccgg tgcagaacag cgtcaacagc actgccggca cgcctattct
     7801 tatgctccac ggcgtctgcc ggacgagaac cctgccgtcg tatatgttcc atatcttatt
     7861 catgatgaat tgacgttgct gcacatacgc atatggccga aagacccgta tgcctgaact
     7921 gtacggaggc gcctcctgat ttttaaatcc gaccaatacc agcctgcacg gctggattct
     7981 atcggctgcc tgcggtttat acaagtttct ttttggctca ttcgtatatc aggacggaag
     8041 ggcagttgac ttttagccgg ccagatgtta catagatgtc atggcaaaag tatcggtaat
     8101 aatccccgga gccggcagag gccttcgttt cggcgggagc atgaacaaga tattcgaact
     8161 cctcggcgat atgccgctgt ttcttcgaac catcgaagca tttataaaac ggcccgacgt
     8221 tcttgaagtg atattcgtct gttcgatgga tgatatggaa tatgtcgctc agcattacgg
     8281 ctggcggctg gagaagctgg gcgtcaagct cgtagccggc ggttctaccc gcacgcagtc
     8341 ggtgaaaaac gcccttgatg ccgtgagcga cgaggctgaa ttggtggcta ttcacgatgc
     8401 agcaaggccg tgcatcgctc agagctggat agatgccgtc ttttcgcaag cctccatgtg
     8461 cggcgcggca ttgctggctg tgccgattca cggaactgtc aaacgcactg acggccgcgg
     8521 aatcgtcacg cagactctgc cccgcgatga atatgccaat cttttcgagg ctcagacccc
     8581 gcaggttttt gcccgcgaaa tcatcatgaa ggtatacgaa aatccggctg aggcgactga
     8641 cgattgcgcg cttgtcgagc aggccggcta tccggtgagt gtggttcacg gggatttgag
     8701 aaacataaaa gtcactcgcc gcgaagacat gatttttgca cggcaggtga tatctacgct
     8761 ttgaaaccgg accggtcatc atggagggcg ggtatgggtt ttagtgacag gaaggttttg
     8821 ctgataaccg cttccgtggg cagcggtcac aatcaggcgg ctcgcagcat ctccgaaggc
     8881 cttgccgagg cgggttgcga ttccatgact attgacgtaa tgtcatatgt gccggcctgg
     8941 ttcagatggt attacagcgg gggatattct ttggcggtat cacgtctgcc gtttttgtac
     9001 ggcgtgggat tccggcttaa cgacatgccg gatttgccgc atcgcaacat cagtgaaaaa
     9061 atccgcatag cttttgaatc ggctgctgct agccggctgg tgcgggcggt tttcgattat
     9121 aaacccgata tcatcgtcaa tacgcatttt ttggcgccgc cgattctcgc gcggctcggg
     9181 tatgacaatc agtttatcgt cgtgaccgac attcaggcgc atcgctggtg gtattcggaa
     9241 ggcattgacc gctggttcgt tcaacagccg cagagtgcga accgcctgat tgaaaagtgg
     9301 aatgttccgc aggggaaaat caccgtcagc ggcatgccgg tgcgcatggc gttcgacacg
     9361 tcccgcagtg acggcgatga tttgcgacgg gaacttcgtc tgccggccgg ccggccggtg
     9421 gtattgctgt ccggcggaac ggtgtttacg tgcgggccgg tgccggcgat agcccgcgga
     9481 atagcgtcga tatgtccgaa tgcatgcgtg gtggtgcttg cagggcggaa caaaaaactg
     9541 ctggccaggc tcggcagcat ggctgaatcg cgtgacggca gggtagtgcc gatgggtttt
     9601 accgacaggg tgagcgattt gatggacgcg gccagcctta tggtgacaaa gtctggcggc
     9661 atgatttgtg ccgaatgtat cgcaaaatcg ctgccggcgg tattcatgcc tcctgttcca
     9721 ggccaggaag cgcagaatgc gaaatatctg gctgaatgcg gcgctgcgag agtggcggaa
     9781 aaattttccg atatcggcgg aatcgtcgcg gagatgctgg ctgacggcga tgggcttgct
     9841 aatatgcaaa aagctgccgg cggacttaaa aggccggcca gaaaaataat cgtcgaaaaa
     9901 atcattgaaa gaatttcaaa ccattgacgc aatgtcagtc cccgctgccg ccgggctgga
     9961 atccgttgga ttaacgcgtc gcaaagtgat aaaataacta tagattgttt agaggagacg
    10021 gcacaatgcg gtatgcaata ataatggcgg gcggttccgg caaacgatta tggccctgtt
    10081 ccagaacgaa cagacccaaa cagctgatac cgatggtgga gggcaaaagt ctgcttgaga
    10141 ttgcggtgga gcgtcttttg ggactttttg agcctgaaaa aatttttata atcaccaatc
    10201 gcgattatgc cgacaaggtg tgtgccgcca ttgataaatt gccggctgag aacataatcg
    10261 gcgagccctc aggccgtgat accgccaacg ccgtagccct ggctgccgcc attatcgaag
    10321 gcagagaccc gcatgccacg atggccattt tcacagccga ccatatcatc cggccggaaa
    10381 gctgctttat cgaagccgtc gaactcgcca tgaagacagc tgaaaaaaat cccgattcac
    10441 ttgttacttt cggcatacgt ccaaattggc cgcacaccgg ccttggttac attgagtgcg
    10501 gcgataccgt agcgccaggc gtgcataagg tggaaagttt cagggaaaag cccgatcatc
    10561 ccaccgcccg caaatacgtc gagtccggcc gatatttctg gaatagcgga atgttcgtct
    10621 ggaaggtttc agccatacag gcggctcttc gggaatttct gccggcttcg gcggcaaaat
    10681 tcgacgcggt taccagggct gtccggaagt cggaggatgt cgggccgatt ctcgataaag
    10741 tgtaccctga actgcagaaa ataagcatcg attatgcggt catggaaaag acgcagggcg
    10801 tgctgatgac tgagcttaat tgcgaatgga tagacctcgg ttcatggccg gcgctggaaa
    10861 acgtgctcga ccaggacgac aagggcaata ccatactggc ggcacatcac gtggttatgg
    10921 acagttttaa caacatcatc gtttccgagg acgagtccca cattatcgcc atgctcggca
    10981 tggacgactg catagtagtg cagtcgggca atgccacgct cgtatgccgc cgcagcgaca
    11041 gtcagcgtct caaggaa
//
LOCUS       DAONFI010000026        10879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6839_length_10879_cov_4.291112, whole genome shotgun sequence.
ACCESSION   DAONFI010000026 DAONFI010000000
VERSION     DAONFI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6839_length_10879_cov_4.291112"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLK08_01570"
     CDS             <1..230
                     /locus_tag="PLK08_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HPS52215.1"
                     /translation="QECRARKAPIIATATAGDTEVAQHANDVIYLPRTSKYAAPITTV
                     VALQMLAYHIAAARGCEIDQPRNLAKSVTVE"
     gene            complement(332..3055)
                     /gene="gyrA"
                     /locus_tag="PLK08_01575"
     CDS             complement(332..3055)
                     /gene="gyrA"
                     /locus_tag="PLK08_01575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPS52216.1"
                     /translation="MADDDQEQYEENMDGQNLPEDDGGDDDDSPIVAAMFPGERIDEL
                     PIEEEMRESYLTYAMSTIMDRALPDVRDGLKPSQRRVLVAMNDLNLGPRSKHRKCAKI
                     AGDTSGNYHPHGEAIVYPTLVRLGQPWNMRYPLIDPQGNFGSIDGDPPAAMRYTEARM
                     KAPAMELLADLNLQTVDLQPNYDDTRTEPTVLPSKFPNLLVNGSQGIAVGMATSIPPH
                     NVTEICDALVKMIDNPDITIDELMEIVKGPDFPTGGLICGRRGIVEGYHTGRGRLCVR
                     AKMHTEQTRGGKEQIVITEIPYQVLKSTIIEHIAETVKAGKIPDIADVQDHSDRTGMR
                     IVVDLKKGAESSVVINQLYKFTPLQSTFSIINIALVNRAPRTLNLRDMLYYFLEHRKD
                     VIRRRTIFLLRRARQRAHILEGLILAVADIDAIIELIKKSPDVPTAKERLMKYQLSLT
                     EDATVRKLLPDSFLNRTVGKPQNLTGVQADAILAMQLQRLTGLEIEKLANDYSRLTEE
                     IEGYEAILTQESLLLDVIREDIFEIKDKYGDERRTQIVQDANEIDIEDLIAEEDVVVT
                     LSHEGYIKRMPVSTYRRQGRGGQGIIGGESKEGDFIEQLFIASTHDYLLVFLNTGKMH
                     WLKVYDIPSMTRTSKGRAIVNLLQMEQNEKICAVVNVREFSDDKYLFIATKKGQVKKT
                     ELSAYENVRKGGLIATGLNEGDQVIGVAITNGNDEIVLGTRDGQAIRFKESDVRPMGR
                     TAAGVRGINIRDEDEVVDMVVVNTHETLLTVCENGYGKRTDFDEYRIQGRGGSGIINI
                     KASDRNGPVVAIKSIHDADELMMITQNGKVVRIAVRELRTIGRATQGVRVISLKPGDK
                     LMAVAKVVPDDDENANQHELPLTPADPQQKLIPDSSEPEENGSPDTVNDEE"
     gene            complement(3357..4160)
                     /locus_tag="PLK08_01580"
     CDS             complement(3357..4160)
                     /locus_tag="PLK08_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPS52217.1"
                     /translation="MGAIVTITSDYGLHDPYVGILKGQIFRHCPDANIVDISHTITPC
                     NILEAAFIIAGAAPYFPAGTLHVVAVDPHGTDRNIIAAQFQGQLYIFPDNGVITLIKE
                     MLPLEAIVAVEPQKFLPREIVDRCHGRDILAPLAGAILKNIGLSRLGHRPHSFKLLEL
                     PKPLAMPNEIVGSVMYVDSFGNMISNITGTLISERWPGAKKLSVVCNTKAVDTVTDNY
                     ASRNPGEAMAVVNPMGLLEIAVVAGRACDTFDAKTGTEIRVSFGKFLDA"
     gene            complement(4274..5038)
                     /locus_tag="PLK08_01585"
     CDS             complement(4274..5038)
                     /locus_tag="PLK08_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPS52218.1"
                     /translation="MDRLDRQKLDSLYRRYNNRRWAELDPIKFLYSYDSAADIEVVGL
                     IAASFAYGRVEQILKSVERILTPLGRQPAKFLLSTPPQEIRRLYPDFRHRFQTSSELS
                     ALLVGIRKTLASDGSLEKAFAAAFAKKPNTPDALTLWRDAIFLAGDSKAGHLLPDPRR
                     GSACKRMFMYLRWMIRKDDVDMGVWKCITPAELIFPLDVHMHRQAMALHATSRKAADL
                     KAALETTESFRKIQPSDPVKYDFALAHQGMDIHRNG"
     gene            complement(5045..5779)
                     /locus_tag="PLK08_01590"
     CDS             complement(5045..5779)
                     /locus_tag="PLK08_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPS52219.1"
                     /translation="MGPIIQALVKLQAIELDLTRIKRRRKAKGNAVTAQENRIAQLND
                     ELSSLKEQSLGKRKKADEFELFLNTNRQRVEKLRTDLNAARTNKEYAAILTQINTQKA
                     DDARVEEEALALMNEIDSLQAQITDIETRTAQETEKLATIKGSCAEEIEKLDRMITEL
                     TEKRNAAAVEVPKEELAIFNRLSNRFEGEAMAEVLVEGKKPPYTYTCSGCYMSLKAEH
                     ANALLSHDKVRLCDNCGRIIYLTALK"
     gene            complement(5991..7118)
                     /locus_tag="PLK08_01595"
     CDS             complement(5991..7118)
                     /locus_tag="PLK08_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPS52220.1"
                     /translation="MSKKISAILKTIIAAFLWLPCSFARAARRYASPAKAAATEQPSI
                     LVVACHWLGDTLWAAQAVPHLRQRWPDAHIAAITKSFAAPLWSGRLSVDEIINGDAIV
                     SDRRRENVDWQAMKKLASQLAARRWDIVIDLTGNRYSAIFCHRLRARYTVGFDGGEFG
                     RLYATCVTGAKQHGEHLSHQPFRVIKPLLGTFFDEEDEYADKNILPPEPQTDYRQACE
                     ILKMAADCPTAIIAPGAGWREKCWAAQNFADIANILLREKWQIVLAGSPQEYELSQKI
                     QKLIYEQFKPSVHLIHEKSVGTILTILSGGAAFLGNDSGLGHIAAAMNLPVIVVFNGQ
                     TDPAACKPLGRLAKYVIAGTPDATPENIAQFFIHMRQERPC"
     gene            complement(7153..8529)
                     /locus_tag="PLK08_01600"
     CDS             complement(7153..8529)
                     /locus_tag="PLK08_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52221.1"
                     /translation="MSTPHLKIFLKHQPVRVWLWIFAIWAVLEAFCVWLRIGLSIEDV
                     PLKFPISICIISPHLKNWPMLAVFCTIAWLVMKNIRQIKTAGLILFSLLLICSGNLMQ
                     GFNGAFLYPLGVETGNTDQYYAEAAGIKISSAEWLADFNENQRDLMTHSRTHPPFAVL
                     LFRELAGAATNDGTCSRWPVLAVWLICTSLCVPMLFEIFHKIGIGINHAKYLAIFLAV
                     MPAFNIYSVLSLDSMIFFLSLFFFHGITDINARGANLENIVAVTISTIAINLLTFGGI
                     FFFVLLLTLAIIRLIRHEKKGYVAALLIAAVVFGAIIIYLNFYCGYDHLRAFLTACGL
                     ESHGRTAAQRVWLYIESRFEDVCEIGLCLGLVTLATMLSAKNRRLDILDFRDRRNEIA
                     VTAIGVLFAIFLGGAYKTGETARTCMFIYPYIFLLMRGLSVRTLREIFVCCTIQTAMM
                     QFMGSYDW"
     gene            complement(8558..9307)
                     /locus_tag="PLK08_01605"
     CDS             complement(8558..9307)
                     /locus_tag="PLK08_01605"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPS52222.1"
                     /translation="MYFQPVKKFDSLASTSIRLREMLNADPTLPGGLVICAAGQIDGR
                     GRFDRKWISPAGDCLTFSALLRIDVPAEYLLSLPMVVSVAVTRMLKKLNIDAKLKWPN
                     DVLVNNRKICGILCENVSMKNCTVPAAIIGVGLNVNLSADAAAEISQPATSMLIETSQ
                     KYSIDGVLNILLEALDAAVSAWAGEGFVAARQTWLANTDDIGRDVEIHSTGEIFRGTI
                     IGLGRFGQLLVKDAEGVSREILEGDVKVTRQ"
     gene            9755..9988
                     /locus_tag="PLK08_01610"
     CDS             9755..9988
                     /locus_tag="PLK08_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HPS52223.1"
                     /translation="MLERIMEEGVVFLCVGMGVVYIFLSVMIWAMGFCAKLSAWLSKF
                     FPEKVPVAKVKKNNNGDDEAAVAAAVAVASVQN"
     gene            10059..>10879
                     /locus_tag="PLK08_01615"
     CDS             10059..>10879
                     /locus_tag="PLK08_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HPS52224.1"
                     /translation="MTRKVNVMVTAFRDGFQSVVGARVFTKDFMPAVEAAHKAGIRHF
                     EFGGGARFQSPFFYCGENAFDSMDEFRRTVGQDVNLQTLARGINVVGLDSQSSDIIDL
                     HAKLFKKHGTTTIRNFDALNDVNNLIFSGQCIKKHGLRHEIVITMMALPPGCEGAHTP
                     EFYIGCLDRILAADVEFDSLCFKDASGTATPSVVYETVRQARAKLGQDMPIVMHTHET
                     AGTSVLVYRSALDAGASQIDLGLNPMSGGTGQPDVLTMWHALRGTEYELGFDVDKL"
BASE COUNT         2359 a         2730 c         2932 g         2858 t
ORIGIN      
        1 tgcaggaatg tcgggcacgc aaagcgccga ttatcgcgac tgcgacggcg ggcgatacgg
       61 aagtggctca gcatgccaac gatgtgatat atctgccgcg gacgtcgaaa tatgccgcgc
      121 caataaccac tgtcgttgcg ctgcagatgc tggcatatca tatagcagcc gctcgcggct
      181 gtgagataga ccagccgcgc aacctggcaa agagcgtaac cgtcgaataa cgtcccaggg
      241 aaccgccctg aaaataaatg gacacaaaaa aagccggtcg tcttattaaa acgaccggct
      301 tttttcagaa agttttttaa aaattatcaa atcattcttc gtcattcacg gtatcgggag
      361 agccgttttc ttccggctca gacgaatcgg ggatgagttt ctgctgcggg tcggcaggcg
      421 tcagaggcag ttcatgctga ttggcgtttt cgtcgtcatc cggaacgact tttgcgacag
      481 ccatgagttt atcgcctggt ttgagcgata tgacacggac gccctgcgtt gcgcggccta
      541 tggtgcgaag ctcccggaca gctattcgta cgactttgcc gttctgcgtt atcatcatga
      601 gttcgtcggc gtcgtggatg gacttgatag ccaccaccgg cccgttacgg tcggatgcct
      661 tgatgttgat aatgccgctg ccgccacggc cctgtatgcg gtattcgtca aaatccgtcc
      721 gcttgccgta gccgttctcg cagaccgtca gaagcgtctc gtgtgtattg actacgacca
      781 tgtcgactac ttcatcttca tcgcgtatgt ttatgccgcg tacgccggct gccgttcggc
      841 ccatcggtct gacgtcggat tccttgaagc gaatcgcctg gccgtcccgc gtgccgagaa
      901 ctatctcgtc attgccgttg gttatcgcca cgccgatgac ctggtcgcct tcattgaggc
      961 ccgtcgcaat aaggccgccc ttgcggacgt tttcatacgc agacagttcg gtctttttga
     1021 cctgtccttt ttttgtggcg atgaacaggt acttgtcgtc ggaaaattcg cggacgttta
     1081 cgacggcgca gattttttcg ttctgttcca tctggagcag atttacgatt gcgcggcctt
     1141 tgctcgtgcg ggtcatcgac gggatgtcgt agaccttcag ccagtgcatc ttgccggtgt
     1201 tcaggaatac cagcaggtaa tcgtgcgtgg aggcgataaa gagctgctcg atgaaatcgc
     1261 cctctttgct ttcgccgccg attatgccct ggccgccgcg gccctgacgg cgataggtgg
     1321 aaaccggcat acgcttgatg tagccctcgt gcgatagcgt aactaccacg tcttcttcgg
     1381 cgatgaggtc ttctatgtcg atttcattcg cgtcctggac aatctgcgtg cgacgttcat
     1441 cgccgtattt gtcctttatc tcaaaaatat cttcgcggat gacgtccagc aggagcgatt
     1501 cctgagtgag gatggcttcg tagccttcga tttcctcggt taatctggag taatcgttgg
     1561 cgagcttctc tatctcaagg ccggtcagcc gctgcaactg catggccagg attgcgtcag
     1621 cctgtacgcc ggtaagattc tgcggcttgc cgaccgtgcg gttgaggaac gagtcgggca
     1681 gaagttttct taccgttgcg tcttccgtaa ggctgagctg gtatttcatc agccgctctt
     1741 tggcggtcgg cacgtcgggc gatttcttga taagttctat aatggcgtcg atatcggcta
     1801 ctgcgagtat caggccttcc aatatgtgtg cccgttgacg tgcgcgacgc aacagaaata
     1861 tggttctgcg gcgtataacg tccttacgat gctctaggaa ataatagagc atgtcgcgaa
     1921 gattcagcgt gcgaggcgca cgattgacaa gggcgatgtt gattatgcta aaagtgctct
     1981 ggagcggcgt aaatttgtaa agctggttga ttacgaccga cgattcggcg cctttcttca
     2041 agtccacgac tattcgcatg ccggtacggt cggagtgatc ctgtacgtcg gcgatgtcgg
     2101 ggattttgcc ggcttttaca gtttcggcga tgtgctcgat aatcgtgctt ttgagaacct
     2161 ggtaaggtat ttccgtaatg acaatctgtt ccttgccgcc ccgcgtctgt tcggtatgca
     2221 ttttagcgcg gacgcacagc cggcctctgc cggtgtggta accttccact atgccgcgac
     2281 ggccacatat cagaccgccc gtcgggaaat ccggcccctt gacaatctcc ataagctcat
     2341 cgatggttat gtccgggttg tcaatcattt ttaccagcgc gtcgcatatt tccgtaacgt
     2401 tgtgcggcgg gatgctggtg gccatgccga ctgctatgcc ctgtgagccg ttgaccagca
     2461 gattaggaaa tttggacggc agaactgtag gctcggtacg ggtatcgtcg taattcggct
     2521 gcaaatccac ggtttgcaga ttcaggtcag ccagcaattc catggctggg gctttcatgc
     2581 gtgcttcggt ataacgcatg gcagccggcg gatcgccgtc gatagaaccg aaattgccct
     2641 gcgggtcgat aagcggatat cgcatgttcc acggctgacc cagccgtacc agagttgggt
     2701 aaactattgc ttcgccatgc ggatgatagt taccggaagt gtcgcctgct atttttgcgc
     2761 acttacgatg cttgctgcgg gggccgaggt taaggtcgtt catggctacc agtacgcgac
     2821 gttgagaagg cttgagcccg tcgcgaacgt cgggcaatgc ccggtccatg atggtactca
     2881 tggcgtaggt gaggtagctc tcgcgcattt cctcttcgat gggaagctcg tctatgcgct
     2941 cgccggggaa catcgctgcg actatcggcg aatcgtcgtc atcgccgccg tcatcttcgg
     3001 gcaggttttg tccatccatg ttctcttcgt attgttcctg atcgtcgtcg gccacttttg
     3061 aactccattc gttatcaaga cgtctcaaat caatgtcgtc tgcatgcgtt tccggtcaga
     3121 ctgccatcgc gatatacgta aaaaccgttt tttaaaattt taaatttaaa atttttaaaa
     3181 aacagtaaaa gcgaatttcc gtacgatgaa atattatgcc ggaaaatgca gatgacgaca
     3241 ccggttcgaa tctggagcag ttctcaaaat ttttttgaga actgctctaa aaaacatagc
     3301 gggaattata tccgaaacga ttccgaaagg caacgaatat cggctgtgtt attgagttat
     3361 gcgtcgagga attttccgaa cgaaacgcgg atttccgtgc cggtttttgc gtcgaaagta
     3421 tcgcaggcac ggccggcgac gacggctatt tccagcaggc ccatgggatt gacgacagcc
     3481 atcgcctcgc cagggtttct ggatgcgtaa ttgtcggtga ctgtatcgac agcctttgta
     3541 ttgcagacta cgctgagttt tttcgcgccg ggccagcgtt ccgagatgag tgtgccggtg
     3601 atgttcgata tcatgttgcc gaagctgtcg acgtacatca ccgagccgac gatttcattc
     3661 ggcatggcga gcggtttggg gagttcgagc agcttgaacg aatgcggccg atggcctagg
     3721 cggctaaggc ctatgttctt gagaatggcg ccagccagcg gagccaggat atcccggccg
     3781 tggcagcggt cgacaatttc acgcggaagg aacttttggg gctctacggc tacgatagcc
     3841 tccagcggca gcatttcctt tatgagcgta attacgccgt tatccggaaa tatgtaaagc
     3901 tggccctgga attgtgcggc gattatgttc cggtctgtgc cgtgcgggtc gacggctacc
     3961 acgtgtagtg tgccggcggg aaagtacggc gccgctccgg cgatgatgaa cgccgcctca
     4021 agtatgttgc agggggtgat ggtgtgggaa atgtcgacaa tattggcatc ggggcagtgc
     4081 cggaatatct ggcctttgag tatgccgacg tatgggtcat gaagaccgta atcactcgtt
     4141 atggttacta tggcgcccat atatccacct catacaacgt aaaaaatcga aaaacgtcag
     4201 tccatgtatg ggaatttata tcagaaacgg tttgccaagt ccagcttgga cgtggaaaat
     4261 cggtcgaatg ccgtcagccg ttcctgtgaa tatccatacc ctgatgcgcc agggcgaaat
     4321 cgtatttgac ggggtctgac ggctgaattt ttcggaaaga ttcggtagtt tccagggcgg
     4381 ctttcaggtc ggctgctttg cgggacgtgg cgtgaagcgc catcgcctgc cgatgcatat
     4441 gaacgtcaag cgggaaaatc agctccgccg gcgtaatgca cttccagaca cccatatcga
     4501 cgtcgtcttt tcgtatcatc catcgcaggt acatgaacat ccttttgcat gcgctgccgc
     4561 gtcgcgggtc gggcagaaga tggccggctt ttgaatcgcc ggcgagaaat atcgcgtcgc
     4621 gccaaagggt caatgcatcg ggcgtatttg gcttttttgc aaaagcggcg gcgaaggctt
     4681 tttcaagtga gccgtccgat gcgagtgttt tccgtatgcc gacaagcaac gccgacaatt
     4741 cgcttgatgt ctgaaaacgg tggcggaaat cgggatacag acgtcgaatt tcctgcggcg
     4801 gcgtagagag aagaaatttc gccggctgac ggccgagcgg cgttaaaatc cgttcgacgg
     4861 atttcagtat ctgttcgacc ctgccgtagg cgaaactcgc ggcaatcagg ccgaccactt
     4921 cgatatcagc cgccgagtcg tatgagtaaa ggaattttat ggggtctaat tccgcccaac
     4981 ggcggttatt gtacctgcga tagagagagt ccagcttttg cctgtccagc cggtccatgg
     5041 cgtcttactt cagcgcggtg agatatatta ttctgccgca gttgtcgcag agacgtacct
     5101 tgtcgtggct gagaagggcg ttggcgtgtt cagccttcag cgacatataa cagcccgaac
     5161 aggtgtatgt gtagggcggt tttttgcctt ccaccagaac ctccgccatc gcttcgccct
     5221 cgaaacggtt ggacagcctg ttgaagatgg cgagttcttc ttttgggact tcgactgccg
     5281 cggcgttgcg cttttcggta agctcggtta tcatcctgtc gagcttttct atttcttccg
     5341 cacatgagcc tttgatggta gcgagttttt ccgtttcctg tgcggtgcgg gtttctatat
     5401 cggttatctg tgcctgaagg ctgtcgattt cgttcatgag tgccagggct tcttcttcca
     5461 cgcgggcatc gtcggctttt tgagtgttta tctgcgtgag gatggctgcg tattccttgt
     5521 tggttctggc ggcgttgagg tcggtgcgga gtttttcaac ccgctgccgg ttggtgttga
     5581 ggaaaagctc aaactcgtcg gctttcttgc gtttccccag cgattgttct ttcaggcttg
     5641 aaagttcgtc gttcaattgg gcgatgcggt tttcctgagc ggtcacggcg tttcccttgg
     5701 ctttccgtcg gcgtttaatc cgcgtcaggt caagctcaat cgcctgtaat ttaaccaatg
     5761 cctgtattat cgggcccatt tgcctgttcc ttcatcttcc atattcggtt tttgtgtcaa
     5821 tatcgggacg aaacagtcct tacttacgta aacagtattt gacgattatg gcgattcgct
     5881 ttgaaaaaac aagggatgat tgattttttt caattatttt atggcgatgg ccgtaaaagt
     5941 tttccccgtt agacttatgt ttgttattga aggtttttag aaccatcctc tcaacatggg
     6001 cgttcctgac gcatatgtat gaaaaattgt gcgatgtttt caggcgtggc gtcgggtgtg
     6061 ccggcgatga cgtatttggc tagcctgccc agtggtttgc aggcggcggg gtcggtttgg
     6121 ccgttgaaga cgacaattac aggcagattc attgctgcgg cgatgtgtcc caagccggaa
     6181 tcattaccca gaaacgctgc cccgcccgat agtatcgtca aaattgtgcc gacgcttttt
     6241 tcgtgtatca gatgtaccga cggtttgaac tgttcataga tgagtttttg aattttttgc
     6301 gacagttcgt actcttgcgg cgagccggcc agcacaatct gccatttttc gcgcagcagt
     6361 atattggcga tgtctgcgaa gttttgcgca gcccagcatt tttcacgcca gcctgcgcct
     6421 ggggctatta tcgccgtcgg gcaatctgcc gccattttca gaatttcgca tgcctgccgg
     6481 taatccgttt gcggctccgg cggcaggatg tttttgtccg catattcatc ttcttcatca
     6541 aaaaatgttc cgagtagagg ttttattacg cggaatggct gatgagacag atgttcgcca
     6601 tgctgttttg cgccagttac gcatgtcgca taaagccggc cgaattcccc gccgtcaaaa
     6661 cccaccgtat acctcgccct cagccggtga cagaaaatcg cactgtaccg attgcccgtc
     6721 aaatcgatga cgatatccca gcgtcgcgcg gctagttgcg atgcgagttt tttcatcgcc
     6781 tgccaatcga cgttttcgcg gcgacggtcg ctgactatgg cgtcgccgtt tatgatttca
     6841 tctacgctca gccggccgct ccacagcggc gcggcgaaag attttgtaat cgcggcgata
     6901 tgcgcatccg gccatcgctg tcgcagatgc ggtactgcct gtgccgccca taacgtatcg
     6961 cccagccagt ggcaggcgac gaccagtatc gaaggctgtt ccgtggcagc cgctttcgcc
     7021 ggcgaggcgt atcttctggc tgcgcgcgca aaactgcacg gtagccacaa aaatgctgcg
     7081 attatcgttt tcaggatagc cgaaattttt ttagacatat aaatataaac cgtatgtttt
     7141 tattcgccgg ttttaccagt cgtatgaacc catgaactgc atcattgccg tctgaattgt
     7201 acagcagacg aaaatctccc gcaacgtccg cacgctcaag ccgcgcatca gcagaaagat
     7261 gtatggataa atgaacatgc aggtacgagc cgtctcgccc gtcttgtaag cgccgccgag
     7321 aaatatcgca aacaatacgc cgatggctgt aaccgcaatc tcgttgcgac ggtctctaaa
     7381 atccaaaatg tcaagccggc gatttttcgc agacagcatc gtcgccagtg tcaccagccc
     7441 caggcagagg cctatttcgc agacgtcttc gaatcgcgat tcgatataca gccatacccg
     7501 ctgtgccgct gttctgccgt gcgactccag cccgcaagcc gttaaaaacg ccctcaaatg
     7561 gtcataaccg caataaaaat tcagatagat tataatcgct ccgaaaacaa ctgccgcgat
     7621 taacaatgcc gccacatatc cctttttttc atgtcgtatc agccggatta tcgccagcgt
     7681 taacaacagg acgaaaaaga aaatcccgcc gaaggtcaga agatttatcg caatcgtcga
     7741 aattgtcact gcgacgatat tttccaggtt tgcaccgcgg gcatttatgt ccgttatgcc
     7801 gtggaagaag aatagcgaca ggaaaaatat catgctgtcc aggctcagca cgctgtatat
     7861 gttgaatgcc ggcatgacgg ccagaaatat agccagatat ttcgcatggt ttataccgat
     7921 tccgattttg tggaatattt caaaaagcat cggcacacag aggcttgtgc aaatcagcca
     7981 tactgcgagt accggccatc tggaacatgt gccgtcattt gtggccgctc ccgccagttc
     8041 gcgaaacagc agcactgcaa atggcggatg cgtacgcgaa tgtgtcatca aatcacgctg
     8101 attttcatta aaatcagcca gccattcggc agaggagatt tttatgccgg cggcctccgc
     8161 ataatactgg tcggtatttc ctgtttctac gccgagcgga tacagaaacg ctccattaaa
     8221 accctgcatg aggttgccgc tgcatatcag cagcaggctg aatagtatca atccggccgt
     8281 tttgatttga cggatgtttt tcatgaccag ccacgctatt gtgcagaaaa ccgccagcat
     8341 gggccagttt ttcagatgcg gtgaaatgat gcatattgaa atgggaaatt ttaacggcac
     8401 atcttctatc gaaaggccga tgcgcagcca gacgcaaaat gcttccagca cggcccatat
     8461 cgcgaaaatc catagccaca cgcgaacggg ctgatgcttt aaaaaaattt taagatgtgg
     8521 cgtcgacatt tttttaacgc ttcgtttttt ttgtttatta ttgcctcgtg acttttacgt
     8581 cgccttcgag gatttccctc gaaacacctt cggcgtcttt taccaggagt tggccgaatc
     8641 ggccgaggcc gattattgtg cctctgaaga tttcacctgt cgagtgaatt tccacgtctc
     8701 tgccgatgtc gtccgtattg gccagccatg tctgccgcgc ggcgacaaag ccttctccgg
     8761 cccatgcgga tacggcggca tccaacgctt ccagcagtat gttcagaacg ccgtcaatgc
     8821 tgtatttctg cgatgtttca ataagcatgg atgtcgcggg ctgtgaaatt tcagcggctg
     8881 catcggctga aaggttcacg ttaaggccga ccccgataat cgccgccggc acggtgcagt
     8941 ttttcatcga cacgttttcg cacaaaattc cgcagatttt cctgttgttc accaacacgt
     9001 cattgggcca tttgagtttt gcgtcgatgt tcagtttttt gagcattctg gttactgcga
     9061 cgctgacgac catcggcagg gaaagcaggt attccgcagg tacgtcgata cgcagcaggg
     9121 cggagaatgt aaggcaatct ccggcaggcg atatccattt tcggtcgaat ctgcctctgc
     9181 cgtcaatttg tccggctgcg catataacga ggccgccggg cagtgtcggg tcggcgttta
     9241 acatttcacg caaccggatg ctggtggaag ctaaagagtc gaattttttc acaggctgga
     9301 aatacatgca ataagctcct gataacgcct tgtattgtaa gaccttgtgg cggcaagtca
     9361 accggcgggc gacattttct gcaaggctat tcgataaaaa gttatcaaaa tattgaaaaa
     9421 atgctcttga aaaatgagtg cggtttaggt tatgctcgaa ttgatttgtg aatcggcagc
     9481 cgtttgcgtc ctctaagtgc ggcctgactt gaacgtttgt ttccgctctc cctctgcggc
     9541 ggaatcatgg caggcccgac cctggtttgc caggaactgc ccatttaatg ccataatggc
     9601 tatcggactg cttacgttct gcggtaaagg tctatccata tgacgccgtt tttacagaat
     9661 taacggcatt atatgacgta ccctgtttga ataagtgaaa aggcgagttt ttttaaaagt
     9721 ctgtaaaagt ctgtaatggc tggagaaaaa gaaaatgctc gaaagaataa tggaagaagg
     9781 cgttgtgttt ctctgcgtcg gaatgggcgt ggtctacatt tttctgagcg tgatgatatg
     9841 ggcgatgggg ttctgtgcga agctgtcggc atggctgagt aagttttttc ctgaaaaagt
     9901 gcctgtggcg aaagtcaaga agaacaacaa tggtgacgat gaggccgccg ttgccgccgc
     9961 cgttgccgtc gccagtgttc agaactgatt ttcgcgatat tttcatgaac tctacaagct
    10021 gtttttcaat tttaacccaa taaacaggat atcggaatat gaccaggaaa gttaatgtaa
    10081 tggtgacggc gttccgcgac ggttttcagt cggtcgtcgg tgcgcgtgtt tttacgaaag
    10141 acttcatgcc tgccgttgag gctgcccata aagccggcat ccggcatttt gaattcggcg
    10201 gcggcgcgcg tttccagtcg ccgttttttt attgcggcga aaacgctttc gacagcatgg
    10261 acgaattccg tcgtacggtc ggccaggatg taaatcttca gactcttgca cgcggcatta
    10321 acgtcgtcgg gctggacagc cagtccagcg atattatcga cctgcacgca aaacttttca
    10381 aaaagcatgg cactactacg attcgcaact ttgacgcgtt gaatgacgtc aataacctca
    10441 ttttttccgg ccagtgcata aagaagcatg gcctcaggca tgagatagtc ataacgatga
    10501 tggctcttcc gccaggctgt gagggtgcac atacccctga gttttacata ggctgcctgg
    10561 acaggatact tgccgcagac gttgaattcg atagcctttg tttcaaggac gcatccggca
    10621 cggcgacgcc gtcggtcgtg tacgagactg tccggcaggc tcgcgccaag ctcggccagg
    10681 acatgcccat agtaatgcat acgcacgaaa ccgccggcac gagtgtgctg gtttacaggt
    10741 ctgcgcttga cgcaggcgct tctcagatag acctgggcct gaatcccatg agcggcggca
    10801 ctggtcagcc ggacgtattg actatgtggc acgcgttgag gggaacggaa tatgagctcg
    10861 gctttgacgt ggataagct
//
LOCUS       DAONFI010000027        10805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_6911_length_10805_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAONFI010000027 DAONFI010000000
VERSION     DAONFI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_10805_cov_4.000000"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..3172)
                     /locus_tag="PLK08_01620"
     CDS             complement(65..3172)
                     /locus_tag="PLK08_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52225.1"
                     /translation="MTTVTTLAKRCAVFCFALGVGLGSLFAAAPVANEYLQDDATIDG
                     RQINFFTDGNEQVAVVNGDFRFEVGRRVISGNQGVVWINTLGKTAAARHDITIYVNGN
                     ARIDDIDSSTTDEMMLVRVHIEGLVTAKSTAVTSNKSLLNSGLYRKARQTRIEELQML
                     AAAKKRASSQGMKIEEKSLVVRKSAQPEANETPAAERTEQNAEKAAEIPLAVTGEKPS
                     KLAAETAPASKAGQTTGGEPSPISVEMAGVKEGKEATPPASVNFEATKGIEVLKDPED
                     PNKRIIVSKGPVYISQGPPKSYQHIELQAQSAVVFTQTKAVGPESDEKVPYAPKLVGA
                     GVNDNETVTGVYLDGDVIISRGERKLMGKTAYYDFIEQKALVLKPVFRTIQEQRNIPI
                     YIRATEARMLGPREIAFNNAIVSTSDFKTPEYSINSKNVKFRDETVYDDNGERLSANR
                     YAVDYDNSTFEVRGVPIFFLPPGQSTFEQEHTALRSVAVGHEGNLGFGAESKWFLFRL
                     LGIVKPKWLDAMLTVSGYEKGAVLGVDYDYAQQEGNRQFSGYGLLYGTYDTNKYDEFG
                     QDNKQLVEHDMRGRILMRHKEYLPKDWEISAEFSYLSDSNYLQEYFADEYYTGKEQEN
                     LVYVKKQRDNWAITGLVNARLNDFLSQTESLPEVAAFLIGQPILDDTMTFYSENRVGG
                     KRWAYSSESPTTSDGAEYANQDSSNTMFRGDTRQEVSLPLRVDTPYGPLNVAPYLVGR
                     VTGWSDTLPSESGGTRPYIQAGVRSNMSFWRVYNNAKSRLFDVNRLRHVITPEVVFYT
                     SAAGGPSQEDLYQLDEGIENINDTTGVLLSLKQRLETKRGAPGQERTVDWMRLNIDFA
                     WFDKNAEYTPGNGTFYFDRPEDSIARRYVGFDYSWNISDRTVLMADAKYDIEEGRLDI
                     ANVGIAIQRDPRLSYFAGFRYIQELEAAVGTVGFDYVINKKYSIRAMEQYDFKFDDGN
                     NLGTSFMIVRRFPRWNVGVDFSYDNRETGEDAVTVMLMLWPEGIPEARLGGGRYNLLS
                     QSNKN"
     gene            complement(3357..4718)
                     /locus_tag="PLK08_01625"
     CDS             complement(3357..4718)
                     /locus_tag="PLK08_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HPS52226.1"
                     /translation="MTPFIAGRVISPLLEFSLRRNTFGIARQMEKTQWLTTQKLRDIQ
                     LEKLRSLVGAALEHTEYYAKFAGVDKSWRPASLDDIRRLPLMDKLTLNSHREELTNRT
                     VRGGVIKSSTGGSSGYPLIFYMDRRRQAWDKAARIRSHEWFGISHGMREAYVWNSPIE
                     LSRNDRLKHFRDWLINERLFAAYDLCEENIAAYVQKLKRFRPSCVFGYPSSMHLLCML
                     AEGAGLRLDDIGVKVVFSTAEVLYEHQREVISRAFGGVPVADGYGSREAGFIAHQCPY
                     GGMHITSENVIVEILRNDGTAATAGEDGEIVVTQLDNYAMPFIRYRTEDVGQLRAGVC
                     RCGRGLELMQVVQGRCNDFLITPHGRRIHSSAVHAMIRVEPGVVKFQVRQAADGSVKI
                     MLVTDEQFSRDAGERIRRKMLERLGPDGIAAVEYCKDIPLPKSGKYRYVISELSAMTG
                     HED"
     gene            complement(4715..6949)
                     /locus_tag="PLK08_01630"
     CDS             complement(4715..6949)
                     /locus_tag="PLK08_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS52227.1"
                     /translation="MSDFIRCRPDGSQAALRAVLVVDTLCSGGKERVVALLASGLAAS
                     GCEVAVITLRRTGTLGLELASRGICVVEIDSSRRFDMRAILRIRRFLKNFRPDVINVH
                     DRASLVYVSAANKGLAGRLIMTCHGLLGGRNHARFDELWASRQVDGVTAVSRDVAGAY
                     VKLLQLRPIVKIIENGIEPARVNRDGGAALRKELGIDNDDMMLLSVGNVKPEKGYEDL
                     IAAAQILSRRNLPKKLRFIVAGNIADEKYYNQLQAMLADANLGQTVKFVGGKADIDTY
                     YSAADVFVMSSRSEGLPMVLLEAMSAGLPSVATAVGGISRVIGDDCGLLCPPAKPEML
                     AASIELMCVDSELRRKVGLAGRRKVLEKYSSSVMTRAYMDYFQKISRHCEYGVVMVSP
                     ALPLEGGMTSVSESLIEGLEHRMTVRGINSGKLTPQHRSIFRGLAAQRNLRRQMLKNI
                     RRMENCICHIHTCSGMSFWRDAWLAAAAGQAGAKVLLHIHGARFDSFMGELGWMMKRR
                     AGKILSRADGVITLGETLRSKYSALQPDENIKWLVVHNGVKIPEKTADGGSGKFLFLG
                     NYSRRKGAADLIKALAVTRDGGNALCANIAGIETQQGQMKQLQNMIDELGIQKQATLT
                     GLLSGNPKYEAITTAEGFVLPSYNEAQPMAILEAMAAGLVVVTTAVGDIPSVVRNGTD
                     GLLVAPGDVNALAAAMLKLSQDTAMRRNMANSARQRVAERYSVEVVTNDLIKIYDNLL
                     GAGK"
     gene            complement(6921..8249)
                     /locus_tag="PLK08_01635"
     CDS             complement(6921..8249)
                     /locus_tag="PLK08_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HPS52228.1"
                     /translation="MGIRDILMTLLGWGCIPVALMDAYYGLLAYCWLSLMKPQSLVWS
                     SSVQENRFVLATAVCMLVRTLTTPLPWWRPKGPAVWFLLFWIWMTICGYLSPNRQAAM
                     EFVLKFTKIAIALMLITGLVRTKEQLKQMVILLAMCPGIYAIRLGLFLLRGGGVTHNG
                     GPLGMDNNDTALFIAMGIPMLLFASYEVQHKWASRGLKITALLAIPAIISGASRGGML
                     AMSIAVAITLWRKFGFIKMILGTLVLGVIALVIIPQATLERYMTIKSYKQDESAMGRI
                     WAWQVARKMADRNPLFGIGLGQTPFIKQYPKYQVHEEDHPHVAHSVWFTTMAGSGYVG
                     LFFYVMMLTSVLLMTRKIRKLTEKGLPDGKNSWAWHYAAMIEATVAAFCVGATFLSQV
                     GFEYVYAICVLTTALWWVTQNELSLAANPLSTGDMRQKEKKNVGLYPLPA"
     gene            complement(8317..9498)
                     /locus_tag="PLK08_01640"
     CDS             complement(8317..9498)
                     /locus_tag="PLK08_01640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS52229.1"
                     /translation="MRWMFVTTRYPWPLANGLWLRLFNIAKELCQLGDTVAVTSFPPE
                     TEFAGMFEQSGAIFLPTDSSQRKDKGPGRYMMSPFIFEQSLAKAVDFHSPYYDVTVLA
                     GPGTLQYSPEARKSPYVLADLIDDPILEYRRRNITSMNVKRFFQQVNFIARYPRYEQM
                     FLEKIDMFTLVSDYDATSFKERNPATEVMVSSNGVDAEYFARPSGYVKSPSRPKVVFL
                     GVMDNINNILAAEYLVKKILPYIVEKCPEVQIQLVGANPVEDVLALDGYNVQVTGTVD
                     DVRPFLWSADAVCLPMVSGTGIKNKLLEAWASGTAVAATPMAVEGIDVRDGEDILVRE
                     NPKELSQAILELLYNDTLRQKLSARALLASRAFNWHMVAARLREAVCPEKYSALLDEG
                     Q"
     gene            complement(9510..10646)
                     /locus_tag="PLK08_01645"
     CDS             complement(9510..10646)
                     /locus_tag="PLK08_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPS52230.1"
                     /translation="MTDKNKLRFDCRHFIGDRPCKFRTVCTCEHYAPVGRRILIIKLG
                     ALGDVVRTACLLPTLRKKYPDSQITWVSRDNGVRILQGHPMIDRLFEFNAENVFPLFY
                     EKFDMVLSLDKERQPAALCNALNSPDKRGMILGEYGTATYANKEAEYYFRLGVDDDLK
                     FRKNVKSYPQLIHEAIGLDYDHTPYELYCSEEDVRQARETLVTVRTTGMPVIALNVGS
                     SRTFANKAPSREKWVEIARRMINIGWQVVLLGGPDEETDKEWIADHLGGKAFRTQSDN
                     SEKQFVALISCCDMLITGDTLAMHVAIARRIPLVVLFGPTCPQEIELYGLGEKIVSTH
                     PCGPCYRRTCDIKPNCMDVIDSGMIIETAGRVMDRCGRKQNRLQ"
BASE COUNT         2196 a         2965 c         2887 g         2757 t
ORIGIN      
        1 gccagcaaaa ttgctcgatt tcggcgacgc ccatgttttt gggataggtt ctaaatttgc
       61 gatgtcagtt tttattgctc tggctgagca ggttatagcg gccgccgccc aggcgagctt
      121 ccggtatgcc ttccggccac agcatcaaca tgaccgtgac tgcatcttca ccggtttccc
      181 tgttatcgta tgagaaatcc acgccgacgt tccaccgcgg gaagcgacga actatcatga
      241 agcttgtgcc gagattgttt ccgtcgtcga atttgaagtc atactgttcc atcgcacgta
      301 tggaatactt tttgttgatg acgtaatcaa atccgacagt gccgacggcg gcttcgagtt
      361 cctgaatata tcggaaaccg gcgaagtagc tcagccgcgg gtctcgctgt atggctatgc
      421 cgacgtttgc gatatccagc cggccttctt ctatgtcgta tttggcgtca gccatcaaaa
      481 ccgtccggtc ggatatattc cacgaataat cgaatccgac gtatctgcgt gctatggaat
      541 cttccggcct gtcgaagtag aaagttccat tgcctggtgt gtattcggca tttttatcga
      601 accatgcgaa gtcgatgttc agcctcatcc agtcgaccgt tcgttcctgg cctggagcgc
      661 cgcgtttggt ttccaaacgc tgcttcaacg acagcagtac gcctgtggtg tcgttgatat
      721 tttcgatgcc ttcgtcgagc tggtacaggt cttcctgact tgggccgccg gcggcactgg
      781 tataaaatac gacttcaggt gttatgacgt ggcgcaggcg gttgacgtcg aacagccggc
      841 tcttggcgtt gttgtagacc cgccagaacg acatgttcga ccgcacgccg gcctgtatgt
      901 atggccgtgt gccgccggat tctgacggca gcgtgtcgct ccagcccgtg acccggccga
      961 ccaggtacgg tgctacgtta agagggccgt agggcgtatc gacgcgcaac ggaagcgaaa
     1021 cttcctgcct cgtatcgccg cggaacatcg tatttgacga atcctggttg gcgtattcgg
     1081 cgccgtcgct ggttgtcgga ctttcgcttg aatatgccca gcgtttgccg ccgacgcggt
     1141 tttcgctata aaaagtcatg gtgtcgtcga gtatcggctg gccgatgagg aatgctgcga
     1201 cttcaggcag agattccgtc tgactgagga aatcgttcag cctcgcattg accaggcccg
     1261 ttatggccca gttgtcacgc tgcttcttga cgtaaacaag gttttcctgc tctttgccgg
     1321 tgtaatattc gtcggcgaaa tattcctgca ggtagtttga gtcgctcaga tagctgaact
     1381 cagccgaaat ttcccagtct ttgggcagat attccttgtg acgcatcagg atacggccgc
     1441 gcatgtcgtg ttcgaccaac tgcttgttgt cctgtccgaa ttcgtcatat ttgtttgtgt
     1501 cgtaagtgcc gtaaagaagg ccataaccgc tgaactggcg attgccttcc tgctgggcgt
     1561 aatcgtaatc cacgccgagc actgcgcctt tttcataacc gcttactgtt agcatggcat
     1621 cgagccattt cggcttgacg atgcccagca ggcggaacag aaaccacttg gattccgcac
     1681 cgaatcccag gttgccctca tggccgacgg ctacgcttcg cagggctgtg tgttcctgtt
     1741 cgaatgtgga ctggcccggc ggcaggaaga atatcggcac gccgcgcact tcaaacgtgc
     1801 tgttgtcata atctactgcg taacggttgg cgcttagcct ttcgccgttg tcgtcataga
     1861 ccgtctcgtc gcggaatttt acgttctttg aattaatgct gtattcaggc gttttgaagt
     1921 cgctggtgga tactatggcg ttgttgaacg ctatttcacg ggggccgagc atgcgggctt
     1981 cggttgcacg gatgtatatc ggtatgttcc gctgttcctg gatggtgcgg aataccggct
     2041 tgagtaccag tgccttctgt tctatgaaat cgtaatatgc ggttttgccc ataagcttgc
     2101 gttcgccgcg cgatatgatt acgtcgccgt cgagataaac gccggtgacg gtttcattgt
     2161 catttacgcc tgcgcctacc agttttgggg catatggaac tttttcgtcg gattcagggc
     2221 ctacagcctt tgtctgcgta aaaaccacag ccgactgcgc ctggagttcg atatgctgat
     2281 aacttttcgg cggcccctgg gaaatgtaaa cggggccctt ggaaacgatg attcttttat
     2341 tggggtcttc ggggtctttg agaacttcga ttcccttggt tgcttcgaag tttaccgatg
     2401 ccggcggagt cgcttccttg ccttccttta cgccggccat ctccacggaa atcggcgacg
     2461 gctcgccgcc cgttgtctgg ccagcctttg atgccggtgc ggtttccgcc gcaagttttg
     2521 atggtttttc acccgttacg gccaggggga tttcggcagc tttttcggca ttttgttccg
     2581 tccgttctgc ggctggcgtt tcattcgcct ccggctgagc ggatttacgt accacgagcg
     2641 atttttcttc tattttcatg ccctggctgc ttgcgcgttt tttggctgca gcgagcatct
     2701 ggagttcttc aatacgggtt tggcgggcct tcctgtaaag gccggaattc agaagcgatt
     2761 tgttcgacgt gacggctgta ctcttggctg ttacaaggcc ttctatgtgt acgcgaacca
     2821 gcatcatttc gtcggtagtg gacgagtcga tgtcgtcaat tctcgcattg ccgttgacgt
     2881 aaatcgttat gtcgtgtcgt gcggcggcgg ttttgcccaa cgtgtttatc cacacaactc
     2941 cctggttgcc ggaaataacc cgtcggccca cttcgaaccg gaaatcgcca tttacgacgg
     3001 caacctgctc attgccgtcg gtaaaaaagt ttatctgtcg gccgtctata gttgcgtcgt
     3061 cctgcagata ttcattggca acgggggcag ctgcaaaaag gctgccgagc ccgactccca
     3121 gcgcgaaaca gaaaaccgca cagcgcttcg ccagcgttgt aactgttgtc atgttatcac
     3181 tcgctctgtt aattaaattg gtacctggca ttaactacaa taacgaacat ggtatcttaa
     3241 cttatgagaa aggccaagta aattcaaaaa accgacgccg gccaactttt ccacacccca
     3301 ataaaacact aacagaaggt gtcgtcgtaa aaattatcaa caggcatgga agctgattaa
     3361 tcttcatggc ccgtcatagc ggaaagctca ctgattacgt agcggtattt gcccgatttc
     3421 ggaagaggga tgtctttgca atattccacg gcggcaatgc cgtccgggcc gaggcgttcg
     3481 agcatttttc ggcggattct ttcgccggcg tcccggctga actgttcgtc ggtcacgagc
     3541 atgattttta cgcttccgtc agccgcctgc cgtacctgga attttacgac gcctggttcg
     3601 actcttatca ttgcgtgtac ggctgagctg tggattcgtc tgccgtgcgg cgttattagg
     3661 aagtcgttgc atctgccctg cacgacctgc atgagttcca ggccgcggcc gcagcggcat
     3721 acgccggctc gtagctggcc gacgtcttcc gtgcgatagc ggatgaacgg catggcgtaa
     3781 ttgtcgagct gcgttacgac tatttcgccg tcctcgccgg cagttgcagc agtgccgtcg
     3841 tttcgcaaaa tttccacaat cacgttttca ctcgttatat gcatgccgcc gtatgggcat
     3901 tgatgtgcga taaatccggc ttcacggctg ccgtagccgt cggctaccgg cacgccgccg
     3961 aaggcgcggc tgataacttc gcgctggtgt tcatataaaa cctcagccgt cgaaaaaacg
     4021 acttttacgc cgatatcgtc cagccgcaaa ccagcccctt ccgccagcat gcaaagcaga
     4081 tgcatcgaac ttggatagcc gaacacgcac gatggcctga accgcttgag tttctgaacg
     4141 tatgccgcga tgttctcttc gcacaaatcg taagccgcaa acagccgttc gtttatcagc
     4201 cagtcccgga aatgttttaa acggtcattg cgggaaagtt cgatgggtga gttccacacg
     4261 taggcttccc gcatgccgtg ggaaatgccg aaccattcat ggctgcggat gcgggctgct
     4321 ttgtcccagg cctggcggcg cctgtccatg taaaaaatca gcggatagcc cgacgacccg
     4381 ccggtgctcg attttatcac tccgccgcga acggtgcgat tcgtcaactc ctctcgatgc
     4441 gaattgagcg tgagcttgtc catgagcggc agacggcgga tgtcgtccag tgatgcgggc
     4501 cgccacgatt tgtccacgcc ggcgaatttt gcataatatt ccgtatgttc cagcgctgcg
     4561 ccgacaagcg agcggagttt ttcgagctga atgtcacgaa gcttctgcgt cgttagccat
     4621 tgcgtttttt ccatctgccg cgcaataccg aacgtattac gcctcagcga gaattcgaga
     4681 agcggcgata tgactctgcc ggcgatgaac ggtgtcattt tccggctccg agcagattgt
     4741 cgtatatttt tataaggtcg ttcgtgacga cttcgaccga atatctttca gcgacccgtt
     4801 gtctggcgct gttggccata tttcgtcgca ttgcggtgtc ctgcgagagt ttcaacatcg
     4861 ctgcggcgag cgcgtttacg tcgccaggcg caacaaggag gccgtccgtg ccgttacgca
     4921 ctaccgacgg tatatcgccg actgccgtgg tgacgacgac gaggccggca gccatggctt
     4981 cgagtatggc cattggctgg gcttcgttgt aactcggcag gacgaaacct tcagccgtcg
     5041 ttatcgcttc atatttcgga ttgccgctga gcaggccggt gagtgtcgcc tgtttttgaa
     5101 tgccgagttc gtcaatcata ttctgcaact gtttcatctg gccctgttgc gtttcaatgc
     5161 cggcgatgtt tgcgcagagc gcgttgccgc cgtcgcgggt gacggctagt gcttttataa
     5221 ggtcggctgc accttttcgg cggctgtaat tgcccaggaa aaggaatttc ccgctgccgc
     5281 cgtctgcggt tttttctggg atttttaccc cgttatgcac gaccagccat ttgatgtttt
     5341 cgtccggctg caaggctgaa tatttcgacc tgagcgtttc gcctagcgtt attacgccgt
     5401 cggcgcggct gagaattttt ccggctcgtc gcttcatcat ccagccgagt tcgcccataa
     5461 acgaatcgaa tcttgcgccg tggatatgca acagaacttt tgcgcctgct tggccggctg
     5521 ccgccgccag ccacgcatcg cgccaaaagc tcatgccgct gcacgtgtga atgtgacaga
     5581 tgcaattctc catccgccgt atatttttca acatctgccg cctgagattc ctttgtgcgg
     5641 cgaggcctct gaaaatggaa cgatgctgcg gcgtaagttt gccgctgttt atgcctcgca
     5701 cggtcatgcg atgttcaagc ccctcgatga gggattccga cacgcttgtc atgccgcctt
     5761 cgagcggcag tgccggactg accatgacca ccccatattc gcagtgtcgc gatattttct
     5821 gaaaatagtc catgtatgca cgcgtcatta cggatgacga atatttttcg agcactttgc
     5881 gtctgccggc gaggccgact tttcgtcgca gttccgaatc gacgcacatc agttcgattg
     5941 aggcggcgag catttccggc ttagccggcg gacagagcag gccgcagtcg tcgccgatta
     6001 cccgtgaaat gccgccgacg gccgtcgcca ccgaaggcag gccggcagac atggcctcca
     6061 gcagaaccat cggcagacct tcgctgcggc tggacatgac aaatacgtct gccgccgaat
     6121 aatacgtgtc gatgtccgct ttcccaccga cgaatttcac cgtctggcct aggttggcat
     6181 cggcgagcat ggcctgcaac tggttgtaat acttctcatc cgcgatattg cctgcaacga
     6241 tgaaacgtaa tttttttggc agatttcggc ggctgaggat ttgggctgcg gcgatgagat
     6301 cttcgtaacc cttttccggt ttcacgttac cgaccgacag cagcatcatg tcgtcattgt
     6361 ctatgccgag ttcttttctg agggctgcgc cgccgtccct gttcaccctt gccggttcaa
     6421 taccgttttc gattattttg acaatcggcc gcagttgcag gagtttcaca tacgcaccgg
     6481 caacatctct ggaaaccgca gttacgccgt ctacctgccg gctggcccat aattcatcga
     6541 atctcgcgtg attgcggccg ccaagaaggc cgtgacacgt cattattaac cggccggcaa
     6601 gccctttgtt ggccgcgctg acgtaaacca gcgaagctcg gtcatgtacg tttattacgt
     6661 cgggcctgaa atttttcaga aaacgcctta ttcgaagaat tgcgcgcata tcgaacctgc
     6721 ggctggaatc gatttccacg acgcatatgc cgcggctggc gagttccagc cccagcgtgc
     6781 ctgtgcggcg aagcgtgatg accgccactt cacagccgct ggcggccagc cccgatgcca
     6841 gaagagccac gaccctctcc ttgccgcctg aacacaatgt atccacgact agaaccgcgc
     6901 gaagcgcggc ttgcgagccg tcaggccggc agcggataaa gtccgacatt tttcttctcc
     6961 ttttgtctca tatcgcccgt agaaagcgga ttcgccgcca gcgacagttc attttgcgtg
     7021 acccaccaca atgccgtcgt caggacgcat atggcgtaaa cgtattcgaa gccgacctgg
     7081 ctgagaaatg tggcgccgac gcaaaatgcg gcgacggtgg cttctatcat tgcggcgtaa
     7141 tgccacgccc agctgttttt gccgtccggc agaccctttt ccgtgagttt tcgtatcttt
     7201 cgcgtcatca acagtacgga tgtaagcatc attacataga aaaacagtcc tacgtaaccc
     7261 gaccctgcca tggtggtgaa ccacacgctg tgggctacgt gcggatggtc ttcttcgtga
     7321 acctggtatt tgggatactg tttgatgaaa ggcgtctggc cgaggcctat gccgaagagc
     7381 ggatttctgt cagccatctt tctggcgacc tgccacgccc atatgcggcc catagccgat
     7441 tcatcctgtt tgtacgactt gatagtcata taccgctcca gcgtagcctg cggtattatc
     7501 acgagtgcta tcacccccag cacgagtgtg ccgagaatca ttttgataaa gccgaatttt
     7561 cgccatagcg tgatggcgac ggctatgctc atggccagca tgccgccgcg gcttgcgccg
     7621 gaaataatcg ccggtatcgc cagcagtgcg gtgattttca accctcggct ggcccacttg
     7681 tgctgaactt catacgatgc gaaaagcagc atcggtatgc ccatcgcgat gaaaagagcc
     7741 gtgtcgttgt tgtccatgcc cagcgggccg ccgttatgcg ttacgccgcc gccgcgaaga
     7801 agaaacaggc ccagccggat ggcgtatatg cccggacaca tcgccagaag aatgaccatc
     7861 tgctttaact gctcctttgt gcggacaagg ccggtaatga gcatcagggc gatggcgatt
     7921 ttcgtgaatt tcaggacgaa ttccattgcg gcttggcggt tgggcgagag ataaccgcat
     7981 atcgtcatcc atatccagaa cagcagaaac cacacagccg gccccttggg ccgccaccac
     8041 ggcagaggcg ttgtgagcgt tcgcacaagc atgcagaccg ccgtggcgag cacgaaacgg
     8101 ttttcctgta ccgatgaaga ccatacgagc gactgaggtt tcatgaggct cagccagcaa
     8161 tacgccagca gcccgtaata cgcatccatc agcgcaacag gtatgcaacc ccagccaaga
     8221 agtgtcatga gtatgtcgcg tatacccatt tttaaaatcc gcaaagaaaa ttcattattc
     8281 cgtttcagaa atccataccg gccaggccgg cggaatctac tggccttcat ccagcagggc
     8341 tgagtatttt tctggacata ccgcctccct cagccgtgcc gccaccatat gccagttaaa
     8401 cgctctgctt gcaagcagag ctctcgccga cagcttctgc ctgagtgtgt cattatacag
     8461 caactccagt atcgcctgcg agagttcttt gggattttcc cgcactaata tgtcttcgcc
     8521 gtctcttacg tcaatgcctt cgacggccat gggcgtcgcc gccacagccg tgccgctggc
     8581 ccatgcctcc aggagtttgt tctttattcc agtgccggag accatcggca gacataccgc
     8641 gtcggcagac cataaaaaag gtctgacgtc gtcgaccgtg ccggttacct gtacgttgta
     8701 accgtctaac gctagtacat cttcaacagg atttgcgcct acgagctgta tctgtacttc
     8761 gggacatttt tcgacaatgt acggcaatat ttttttcacc aggtattccg cagcgaggat
     8821 attgttgata ttgtccatta cgcccagaaa caccactttc ggccggcttg gggattttac
     8881 gtatcctgag ggccgtgcaa aatattcagc atccacaccg ttcgacgaga ccatgacctc
     8941 cgtcgcaggg ttgcgctcct tgaaacttgt ggcgtcataa tccgaaacca gtgtgaacat
     9001 gtcaatcttt tcgaggaaca tctgttcgta tcgcggataa cgggcgatga aattgacctg
     9061 ttggaaaaat cgcttgacgt tcatgctggt tatgttccgg cggcgatatt ccagaatcgg
     9121 gtcgtctatg aggtcagcca ggacgtaggg tgattttctg gcttcaggtg aatattgcag
     9181 agtgcccggc ccggccagca ctgttacgtc gtaatacggc gaatggaaat cgacagcctt
     9241 ggccaatgac tgttcgaata tgaaaggact catcatataa cggccggggc ctttatcttt
     9301 ccgctgactg gagtcagttg ggagaaaaat cgcgccgctc tgttcgaaca tgccggcgaa
     9361 ttccgtctca ggcggaaaac tcgtcaccgc cacggtatcg ccgagttgac ataactcttt
     9421 tgcgatattg aacaatctca gccacaggcc gttcgcaaga ggccatggat accgtgttgt
     9481 aacaaacatc caacgcatat agaatcaacc tattgcagac ggttctgttt tctgccgcac
     9541 ctgtccatga cacggccggc tgtttctatt atcatgccac tgtctatgac gtccatgcag
     9601 ttgggcttga tatcgcacgt gcgtctgtaa cacgggccgc atggatgtgt ggaaacaatt
     9661 ttttcgccga ggccgtaaag ttctatctcc tgggggcacg tcgggccgaa gagaaccacc
     9721 agcggtatcc ggcgggcaat tgccacgtgc atggccagcg tatcgccggt tatcagcatg
     9781 tcgcaacagc ttatcagtgc gacgaattgt ttttctgaat tgtcggattg cgttctgaag
     9841 gctttgccgc ccagatggtc ggctatccat tccttgtccg tctcttcatc cgggccgccc
     9901 agcaggacta cctgccagcc gatgtttatc atgcgcctgg cgatttcgac ccacttttcc
     9961 cgcgacggag ccttgttcgc gaatgtgcgg gatgagccca cgttaagcgc aatgaccggc
    10021 atgcccgtgg tgcggacagt tacgagcgtc tcgcgagcct gccgtacgtc ctcttcgctg
    10081 caatacagtt cataaggcgt atggtcgtaa tcaaggccga tggcttcatg tatgagctga
    10141 gggtagcttt tgacgttttt gcggaattta aggtcgtcat ccacgccgag gcggaaataa
    10201 tattcagcct ccttgttggc gtatgtggcc gtgccgtact cgcctagtat catgccgcgt
    10261 ttgtcaggcg agttgagggc attgcagagg gcggctggct ggcgttcttt gtcgagtgat
    10321 aaaaccatgt cgaatttttc gtaaaaaagc ggaaacacgt tttcggcgtt gaactcgaac
    10381 agacggtcta tcatcggatg gccctggagt atgcgtacgc cgttgtcgcg ggaaacccat
    10441 gttatctgag agtcaggata tttttttctg agcgtcggca gaagacatgc cgtgcgaacc
    10501 acgtcgccca gtgcgcccag cttgataatc agtatgcgtc tgccgaccgg agcgtaatgt
    10561 tcgcaagtgc agacggttcg gaacttgcag ggcctgtcgc ctatgaaatg ccggcaatcg
    10621 aatctcaatt tatttttatc tgtcatcaat actcgcaatc gtcatttcat taccagcgtt
    10681 tcgacggcgg taacgtaatt ttctacgcac ctgttccacg tgaactcttt tacctgcgaa
    10741 aggcctctgt cgacgagtga ttcccgcagg ctggcatcgg tcataattct tctcatggcg
    10801 tgagt
//
LOCUS       DAONFI010000028        10619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7086_length_10619_cov_3.262022, whole genome shotgun sequence.
ACCESSION   DAONFI010000028 DAONFI010000000
VERSION     DAONFI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7086_length_10619_cov_3.262022"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1511
                     /gene="hydG"
                     /locus_tag="PLK08_01650"
     CDS             90..1511
                     /gene="hydG"
                     /locus_tag="PLK08_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPS52231.1"
                     /translation="MKLFENAKLSKGAVDFINDDYIESLINAGPSDATKVREIIAKSM
                     EKKPLTVEEASALLNADEKLSEEIFSAARELKKRVYGNRIVLFAPLYVGNYCINNCKY
                     CAFKKSNTEQIRRTLTDEELRQEITALENMGHKRLIMVYGEHPKYDGKYIAHTVETAY
                     GVKVGHGSIRRININAAPMDHENYRIIKAAGIGTYQIFQETYNHKIYAAMHPADTTKG
                     SFTWRLDGLSRAFEAGIDDMGIGALIGLGDWKFDALGLVSHAIYLQKHHGVGPHTISF
                     PRIQPAAGVKMDESKFPNNYEFKKMVAMLRLAVPYTGLILTARESADIRRDLIEFGIS
                     QIDAGSRIDIGGYSLLGKTQELKREQFQLGDVRSLDNVVRELAQSGNIPSWCTACYRL
                     GRTGEHFMEFAIPGFIKRYCTPNALSTFTEYLTDYASPETKQVGLKLVQDELKKMEES
                     PMKKELINRLERIQTTDDRDLYF"
     gene            complement(1623..2429)
                     /gene="trpA"
                     /locus_tag="PLK08_01655"
     CDS             complement(1623..2429)
                     /gene="trpA"
                     /locus_tag="PLK08_01655"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPS52232.1"
                     /translation="MMNRIDVKFQKLRENGQKALVAFISAGDPDFDVSLEIITQMCKS
                     GVDVLELGVGFSDPTADGPVIQRASQRAIKAGMSLTRSLEMVKLLRSRVDTPIVIFSY
                     YNPVFHYGVEKFCRDAVKAGVDGTLLVDLAYEESAEFTNVRGNLDIKLVRLIAPTTSN
                     ARAAKICSAAEGFVYLISRTGVTGTGGLDYSEVESHVREVKAMTGLPVCLGFGVSTAE
                     DVRKISAYADGVVIGSAFEKIIEANLADVKRLPEMLAQKTAEFKAATIQK"
     gene            complement(2426..3640)
                     /gene="trpB"
                     /locus_tag="PLK08_01660"
     CDS             complement(2426..3640)
                     /gene="trpB"
                     /locus_tag="PLK08_01660"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPS52233.1"
                     /translation="MSKADIKKILASPDERGHFGEFGGQYVGESLMSALEELSAAFSK
                     YRYDDGFWAEYRYLMRDYVGRPTPVYFAERLSSHIGGAKIYLKREDLAHTGAHKVNNT
                     IGQALMAKYMGKRRVIAETGAGQHGVATATVAARFGMDCKVFMGVEDIRRQQPNVKRM
                     KLLGAEVVGVESGTGTLKDAMNEAMRYWSQVVRDTFYVIGTVAGPAPYPEMVREFQKI
                     IGIEAREQMLEHIGRLPDAVIACVGGGSNAIGAFYAFIPDDSVRLIGVEAAGRGVQTG
                     QHAASIGHGRIGVLHGSKSYVLQDENGQIIEAYSISAGLDYPGVGPEHSFLKDAGRAE
                     YPSVTDAEALEAFHLLCRYEGIIPALESSHALAYAAKEAKKLGSDKILLVNLSGRGDK
                     DLGHFDKPTEDK"
     gene            complement(3640..4287)
                     /locus_tag="PLK08_01665"
     CDS             complement(3640..4287)
                     /locus_tag="PLK08_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPS52234.1"
                     /translation="MQVKICGLRDVVQAEACVRLGADAIGLVFYPQSPRFVEISKAVE
                     ICHAVAGHVARVGVFVDMSADEIIDHVKKCGLDVAQLHGSETAELVADLKAAGVRVVK
                     VLNSTGDDLRRDAAKFAGAADSFLVEAGKGVLPGGNAATWNWAGAGTLREVASFALAG
                     GLCAENVGDAINAAGCDAVDVSSGVEISPGVKDMKKVKMFINRAKSLNIDGRRVF"
     gene            complement(4290..5084)
                     /gene="trpC"
                     /locus_tag="PLK08_01670"
     CDS             complement(4290..5084)
                     /gene="trpC"
                     /locus_tag="PLK08_01670"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPS52235.1"
                     /translation="MERNILKEIVELKRGEVTELKKSQPLADLKERIERRRHHRDFYA
                     AMADSADDVRIIAEIKRASPSRGIIDEDIDPAKVAVDYQIGGAKAISILTEREFFMGS
                     LQDLEAVRDACSLPILRKDFTIDEYQIYESAAVGADAILLIVRLLEKQQLKDYIALAT
                     QLGIDCLVEIHHDSDVEKLEGSGAKIVGINNRDLQTFETSLDVAIRMAGMLGGQYLAV
                     AASGIFGRDDIDANLAAGIHCFLIGESLVRAADSVAFLKELRGVDA"
     gene            complement(5089..6099)
                     /gene="trpD"
                     /locus_tag="PLK08_01675"
     CDS             complement(5089..6099)
                     /gene="trpD"
                     /locus_tag="PLK08_01675"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPS52236.1"
                     /translation="MNFSTTLSKIISHESLTRQDVSDVFSAVFSGDLTPAQMGGFLCA
                     MSTKGVTFEELAGAASAMRAKASRIQVIAKPVVDTCGTGGDGADTFNISTVAAFVVAG
                     AGVTVAKHGNRSASSQCGSADVLEELGLNLDADPEVVEEAVNTIGIGFMFARKFHGAM
                     KYAASVRRELGVRTMFNMLGPLTNPAAAGCQVLGVYSPDLTETFANALGLLGVKRAFV
                     VHGHDGLDEITVCDKTRVSELCGGDVKTYEIYPEQYFGELAAADALRGGDRHVNAQIA
                     RDVLSGRKGPRRNVVLINAAAALVAAEKADNIDAGIKLAEQSVDSGAAAEKLQKLIEY
                     MR"
     gene            complement(6112..6693)
                     /locus_tag="PLK08_01680"
     CDS             complement(6112..6693)
                     /locus_tag="PLK08_01680"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPS52237.1"
                     /translation="MIIMIDNYDSFTYNLVQYLRQLGAEVEVYRNDEITVEEIAARDP
                     AGIVISPGPSIPQNAGISVDVVKYFAGKIPLLGVCLGHQAIGYAFGANIVHARHLMHG
                     KTSNIDTDGKGVFAGIKGSVQVMRYHSLAIEEATLSKDFEITARADDGEIMGVRHRTL
                     PVEGVQFHPESIMTPAGKRMLRNFLKQCGEITE"
     gene            complement(6721..8199)
                     /locus_tag="PLK08_01685"
     CDS             complement(6721..8199)
                     /locus_tag="PLK08_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPS52238.1"
                     /translation="MFIRSFPDRKGYEKLASEHNVVAVCSEILADMDTPVSLLRKFFC
                     PGRDCFLFESMEGTARWGRYSFLGLSSRARVLVYRDRVEIHNGVQREVIPHGGDPLAV
                     LREFMSRFKVALSPELPRFQGGMVGYFTYETVSFFENIPNRLDENAVMAEFMMPDEMI
                     IFDNVRHTLMCVVLTFIGKGDDISLCYDNAVQRLTKMTDDIQRPFRAIEVRRQRDLNL
                     RPVEPDDVYRKNVAKTKEMIFDGEIIQAVIAQSFEADAPGDPTALYRAMRFINPSPYL
                     YFMHFGGMTICGASPETMVRLEGTRACVRPIAGTRPRGCDETQDRILADELLKDEKEK
                     AEHLMLVDLGRNDLGKVAVTGTVQVTDLMFIERYSHVMHLVSNVECDMREGLDCFDLF
                     RATFPAGTLSGAPKVRAMEIIAGLEKTPRGPYGGAVGYIAFGGNMDFAITIRTAVIEG
                     EKLTVHAGAGIVADSDPETERMETVNKARAIERALHFIGDRC"
     gene            complement(8451..9209)
                     /locus_tag="PLK08_01690"
     CDS             complement(8451..9209)
                     /locus_tag="PLK08_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPS52239.1"
                     /translation="MVQMIRTISVIGGRGAMGRRFVDMFRAQGLTVRSFDSSDGEMDY
                     AAAAAADVVVLAVPIRCFEEVVVKLGPHTRKDGLVADITSVKELPVKIMLNHCRGEVL
                     GGHPLFGPAEKNLEGQIFFLCPARLGNLTTRFKSLLKRLKFRIIVLTAAEHDRQMSIV
                     QSLRHMMMAGFGATIAKFGDAGQLVNVGDWFGRLKELRDRQASQSAELFADIALNNPH
                     SREVFDEFAAAVERLKNALDKNDRKEMIELMKSI"
     gene            complement(9209..10303)
                     /gene="pheA"
                     /locus_tag="PLK08_01695"
     CDS             complement(9209..10303)
                     /gene="pheA"
                     /locus_tag="PLK08_01695"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPS52240.1"
                     /translation="MAGELENLRKQINELDNELLELLQKRAEVALAIGQTKRVVGMEV
                     CDPAREREVMRRLKSSIHGPLVPDSIEHIFREIISACRGLQANVRVAFLGPEATFSHL
                     AAMRRFGECACFVPSESIEDVFRKVESGNVEFGVVPVENSTNGTVGETFDAFLHSPLQ
                     ICGEMCLRISNSLLSKAASLGDIRRVYSHPQPIAQCRNWLTKYLPNAVCVALESTAKA
                     GERAAAEPDAAAIGGELVREKYGLTALASDIQDRSSNQTRFWVISRESCYPTGHDKTS
                     LRFAASHKPGSLFRCLGVFAENGVNITRIESRPSPSHVWEYVFFLDIEGHRSDEPVAK
                     SLTTLSDMVGQIYVMGSYPTSEDFMAGEKR"
     gene            complement(10323..>10619)
                     /locus_tag="PLK08_01700"
     CDS             complement(10323..>10619)
                     /locus_tag="PLK08_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPS52241.1"
                     /translation="RTFETATRNTLDLSAIPVIKKKSHLPVIVDPSHAVGISEFVAPL
                     AKASLAVGADGLMIEVHNNPCQALCDGDQSITPDAFGELMKVLSRMADVMDMRM"
BASE COUNT         2443 a         3163 c         2629 g         2384 t
ORIGIN      
        1 ccgctgcctt gaatcgcgac ttgcgtccat aggccgcacc gtcggcaggg gcaggggtga
       61 ctctgcaaat ttcaccagga gaacctatca tgaaactgtt tgaaaacgcc aaactcagca
      121 aaggcgccgt cgatttcata aacgacgact acattgaatc actcatcaac gccggcccat
      181 ccgacgcaac gaaagtacgg gaaataatcg ccaaaagcat ggagaaaaag ccgctgaccg
      241 tcgaagaggc ctccgccctt ctcaatgccg acgaaaaact gtcggaagaa attttctccg
      301 ccgcacgtga actcaagaaa cgcgtctacg gcaaccgcat cgtgctgttc gcaccgttgt
      361 acgtgggcaa ttactgcata aacaactgca aatattgcgc gttcaagaag tccaacacgg
      421 aacagattcg ccgcacgctc accgacgagg agctcaggca ggagataacg gcattggaga
      481 acatgggcca caaacggcta atcatggtct acggcgaaca tccgaaatat gacggcaaat
      541 acattgcgca tacggtggaa accgcctacg gcgtaaaggt cggccacggg tcaatacgtc
      601 gcattaatat caatgccgca cctatggatc acgaaaacta ccgcatcatc aaagccgccg
      661 gcatcggcac ataccagatt ttccaggaaa cgtacaatca caaaatttat gcggcgatgc
      721 atccggcaga caccaccaag ggcagcttca catggcggct ggacggcctc agccgcgcgt
      781 tcgaagccgg catcgacgat atgggcatcg gcgcactcat cggcttgggc gactggaaat
      841 tcgacgcact gggccttgtg tcgcacgcta tatatctcca gaagcatcac ggcgtaggcc
      901 cgcataccat aagttttcct cgcattcagc cggcggctgg cgtcaaaatg gatgagtcga
      961 aattccccaa caattacgaa ttcaagaaaa tggtcgcaat gctgcggctg gcagtgccgt
     1021 acaccggcct gatacttaca gcccgtgaaa gcgccgatat ccgccgcgat cttatcgaat
     1081 tcggcatatc gcagatagac gccggcagca gaatcgacat cggcggttac agcctgctcg
     1141 gcaagacgca ggaactcaaa cgcgaacagt tccaactggg cgacgttcgc agccttgaca
     1201 acgtagtccg cgaactcgca cagagcggca atataccgag ctggtgcaca gcctgctacc
     1261 ggctgggcag aacaggcgaa catttcatgg aattcgccat tccaggcttt ataaaacgtt
     1321 actgcacgcc aaacgcactg tcgacgttta cggaatacct caccgactac gcatcgccgg
     1381 aaacaaaaca ggtcggcctt aaactcgtgc aggacgaact taaaaagatg gaagagtcgc
     1441 ctatgaaaaa ggaactcatc aacagacttg aacgcataca gaccaccgac gacagggatt
     1501 tgtattttta gaacctaaaa tttttatata agaacgtggt tttatagcgg ttctctaaaa
     1561 ttttttttta gagaaccgct tttataatcc cttgcggaac ttttatgcat acatggtgga
     1621 atttactttt gtattgttgc ggctttgaat tcggcggttt tttgggcaag catttccggc
     1681 agtcgtttaa cgtcagcaag attggcttcg attatttttt caaatgcgct gcctattacg
     1741 acgccgtcgg catatgctga aatttttctg acatcttctg cagtggacac gccgaaaccc
     1801 agacataccg gcaggccggt catcgccttg acctcgcgaa cgtgcgactc cacttcgctg
     1861 tagtctagtc cgccggttcc agtaacgccg gtgcgagaaa taaggtacac gaatccttca
     1921 gcggcggaac atatctttgc agccctcgcg tttgaagtcg tcggcgcaat gagacgtacg
     1981 agctttatat ccagattccc gcgaacattc gtaaactcag ccgactcttc ataggcaagg
     2041 tcgaccagca gcgtgccgtc aacgccggcc ttcacggcat cgcggcagaa tttttcaacg
     2101 ccgtaatgaa ataccgggtt gtaatagctg aaaatcacta taggcgtatc cacgcgactg
     2161 cgaaggagtt tgaccatttc gagcgagcga gtaaggctca tgccggcttt tatcgcgcgt
     2221 tgtgaagcac gctgaatcac ggggccgtcg gcagtggggt cggaaaatcc tacgccgagt
     2281 tccagcacat ccacaccgga cttgcacatt tgcgttatta tttccagcga gacatcaaaa
     2341 tccggatcgc cggcggatat gaacgccacc agcgcctttt ggccgttctc gcgaagcttt
     2401 tgaaatttta catctatcct gttcatcatt tatcctcggt tggcttgtca aaatgtccca
     2461 aatccttgtc gccgcggccg gagaggttca ccagcagtat tttgtcactg ccgagttttt
     2521 ttgcctcttt ggccgcatag gccagcgcgt gagaactttc cagcgccgga atgataccct
     2581 catacctgca cagcagatga aacgcctcca gcgcctcggc atccgttacg ctgggatact
     2641 ctgccctgcc ggcgtccttg aggaacgaat gctcaggccc tacgccgggg taatccaagc
     2701 cggcggatat cgaatatgcc tctataatct ggccgttctc gtcctgcaac acataagatt
     2761 tcgaaccgtg cagcacgcca atccggccat ggccgattga cgccgcgtgt tggccggtct
     2821 gaactccacg gccagccgcc tcaacgccga ttaatctgac actatcgtca ggaatgaatg
     2881 cgtaaaacgc gccaatggcg ttactgccgc cgccaacgca ggcgatgact gcgtctggca
     2941 gacggccaat gtgctccagc atctgctcgc gagcctcaat gccgattatc ttctgaaatt
     3001 cgcgaaccat ctcagggtac ggcgccgggc cggcgacagt gccgatgacg taaaacgtat
     3061 cccgcaccac ctgcgaccag tatcgcatcg cctcattcat ggcgtccttc agcgttcccg
     3121 tgccgctttc cacgccgacg acctccgcgc cgagcagttt catgcgtttg acgtttggct
     3181 gctggcggcg gatgtcttca acacccatga acaccttgca atccatgccg aaccgcgccg
     3241 caacagtagc cgtcgccacg ccatgctggc cggcaccggt ctcggctatc acgcgacgct
     3301 tgcccatgta cttggccata agtgcctggc cgatagtgtt attgaccttg tgcgctccgg
     3361 tatgcgccag gtcttcacgc ttcagataaa tcttcgctcc gccgatgtga ctgctgagac
     3421 gctcggcaaa gtaaaccggc gtcggccggc cgacgtaatc ccgcatcagg tagcgatact
     3481 cggcccagaa cccgtcgtca tagcgatatt tcgaaaaagc cgccgacaac tcctccagcg
     3541 cactcatgag cgattcgccc acatattggc cgccgaattc gccgaaatgg ccccgctcat
     3601 ccggcgaggc taaaatcttt tttatatctg ccttggacat cagaaaactc tcctgccatc
     3661 aatattcaat gattttgctc tgttaatgaa cattttaacc tttttcatat ctttcacgcc
     3721 aggcgatatt tcaacgccgc tgctgacgtc taccgcatca cagccggcgg catttatcgc
     3781 atcgccaaca ttttccgcac acaatccgcc ggcaagggca aacgacgcga cctcacgaag
     3841 cgtccctgcg ccagcccaat tccatgtcgc ggcattgccg cccggcagaa cgcctttgcc
     3901 ggcttcgacg agaaacgagt ccgccgcgcc tgcgaacttc gccgcatctc tccgcaaatc
     3961 gtcgccggtg ctgttgagaa cttttaccac tcttacgccg gcggctttca agtcggcaac
     4021 cagttccgca gtctcactgc cgtggagttg tgccacatcc aggccgcatt tttttacatg
     4081 gtcgataatt tcatcggctg acatatccac aaatacgccc acacgtgcga catgtcccgc
     4141 aacggcatga cagatttcga cagccttcga aatttccaca aatctcgggc tttgggggta
     4201 aaaaaccagt ccaatcgcat ccgcaccgag tcgcacgcac gcctcagcct gcacgacgtc
     4261 tctcaagccg caaattttca cctgcacact caagcgtcaa ctccacgcaa ttctttaaga
     4321 aatgcaacag aatcagccgc cctgacgaga ctctcgccga tgaggaaaca gtgtatgcct
     4381 gcagccagat tcgcgtctat gtcgtcacgg ccgaaaattc cgctggcagc taccgccaga
     4441 tattgcccgc ccagcatgcc agccatacga atcgccacat caagagaagt ctcgaacgtc
     4501 tgcaaatcac gattgtttat gccgacgatt tttgcgccgc tgccttcgag tttctccacg
     4561 tcgctatcgt gatgaatttc caccaggcag tcaatgccca gttgcgtcgc gagagctatg
     4621 tagtccttga gctgctgttt ttcaagcaga cggacgataa gcagaatagc gtccgccccg
     4681 acggcggcag attcgtaaat ctggtattcg tctatcgtaa aatccttgcg gagtatcggc
     4741 aaggagcagg cgtcgcggac ggcttcaagg tcctggagcg aacccatgaa gaattcccgc
     4801 tccgtcagga tggatatcgc ttttgcgccg cctatctggt aatcgacggc taccttcgca
     4861 gggtcgatat cttcgtcgat tatgccgcga gaagggctgg cccgcttgat ttccgcgatt
     4921 attcgcacgt cgtccgcgga atcagccatc gcagcataaa aatcgcgatg atgcctgcgg
     4981 cgttctatcc gctccttcag gtcagccagc ggctgggact tttttaattc cgtcacttcc
     5041 ccgcgtttga gttcaacaat ctctttcaga atattccgtt ccatagccct acctcatata
     5101 ttctataagt ttttgcagtt tttcggcagc agcaccggaa tcgacggact gttcagccag
     5161 tttgattccc gcgtcaatat tatcggcttt ttcggcagcc accagcgcgg ctgcggcgtt
     5221 tataagcacg acgtttctac gcgggccctt ccgccctgat aaaacgtcgc gtgcgatttg
     5281 agcgttgaca tgcctgtctc cgccgcgaag agcatcggct gcggccagtt cgccaaaata
     5341 ttgttccgga taaatttcat acgttttgac gtcgccgccg caaagttcac tgacccgcgt
     5401 cttgtcgcag acggttattt cgtccaaccc gtcatggcca tgcactacga acgcgcgctt
     5461 cacgccaagc agcccaagcg cattggcgaa agtctccgta aggtcaggcg aatacacacc
     5521 cagcacctga cagccggcag cagccggatt cgtcaggggg ccaagcatat tgaacatcgt
     5581 acgaacgcct agttcacgtc gcacggatgc cgcatatttc atcgcgccgt gaaattttct
     5641 ggcgaacatg aacccgatgc cgattgtgtt tacagcttct tccacgacct cagggtcggc
     5701 gtccagattc agccccagtt cctccagcac gtcggcactg ccgcactggc tggacgcgct
     5761 gcgattgccg tgcttggcga ctgtcacgcc ggcgccagcc accacaaaag cagccacagt
     5821 tgaaatgttg aacgtgtcgg ctccgtcgcc gcccgtgccg caggtatcca caaccggttt
     5881 ggcgatgacc tggatgcggc tggctttggc acgcatggcg gaagcggctc cggcgagttc
     5941 ctcaaacgtg acgccctttg tcgacatcgc gcacaggaat ccgcccatct gtgcaggcgt
     6001 gaggtcgccg ctgaaaacgg cactgaaaac gtcagacaca tcctggcgcg tcaacgattc
     6061 atgcgatatt atctttgaaa gtgtggtgga aaaattcatg cgtcgtctcc gtcattccgt
     6121 aatttcgccg cactgtttga ggaaattgcg caacatacgc ttgccagccg gcgtcatgat
     6181 ggattccgga tgaaactgca cgccctcaac aggaagcgtc ctgtgacgca cgcccatgat
     6241 ttcgccgtca tcggcacgtg cggtaatttc aaaatccttt gagagcgtcg cttcctcgat
     6301 agccagcgaa tgataccgca ttacctgcac gctgcccttg atgccggcga atacaccctt
     6361 gccgtccgta tcgatgttgg acgtcttgcc gtgcataagg tgtcgggcat gtacgatgtt
     6421 agccccaaaa gcgtaaccga tggcctgatg gcccaggcag acgcccagca acggaatttt
     6481 tccggcaaaa tatttgacta cgtccacact tatgccggcg ttctgcggaa tgctggggcc
     6541 aggcgatatc acaatgccgg ccgggtccct tgcggcaatc tcctcgaccg ttatctcgtc
     6601 attgcgataa acttccactt ccgcgcccaa ctgccgcaga tactgcacaa ggttgtacgt
     6661 gaacgaatcg taattgtcta tcattattat cattttataa attccttccg gcaaaaatct
     6721 tcagcacctg tcgccgataa aatgcagggc ccgctcgata gcacgcgcct tgttgacggt
     6781 ttccatgcgt tcggtttcag ggtcgctgtc agcgactatt cctgcgccgg cgtgtacagt
     6841 aagtttttct ccttcgatga cggcggtacg gatggttatg gcgaaatcca tattgccgcc
     6901 gaaagcgata tatcctactg ctccgccata cggcccgcgc ggagtttttt ccagtccggc
     6961 gattatctcc atcgcccgaa ctttcggcgc gccgctgagc gtgccagccg gaaatgtcgc
     7021 cctgaacaga tcgaaacagt ccagcccctc gcgcatatcg cattcaacgt tgcttacaag
     7081 atgcatgaca tgcgaataac gctcgatgaa catcaggtcg gtaacctgaa ccgtgccggt
     7141 cacagccacc ttgcccaggt cgtttcggcc aagatctacc agcatgagat gttcagcctt
     7201 ttccttttca tccttgagca gttcgtcggc aaggattctg tcctgagttt catcacagcc
     7261 gcgcggcctt gtgccggcaa taggccgcac gcacgcacgc gtcccttcca gacgaaccat
     7321 cgtctcaggc gacgcaccac atatcgtcat gccgccgaaa tgcatgaaat aaagataggg
     7381 tgaaggatta atgaatcgca tggcacgata aagagccgtc gggtcgccag gagcgtcagc
     7441 ttcgaacgac tgggcaataa ccgcctggat tatctcgccg tcgaaaatca tctccttggt
     7501 tttggccaca tttttacgat agacgtcatc aggttcaacc ggccgcaggt tcaggtcccg
     7561 ctgacggcga acctcaatag ccctgaatgg ccgctggatg tcatccgtca ttttagtcag
     7621 ccgctgcaca gcattatcat aacaaagcga aatgtcgtcg cccttgccga taaacgtaag
     7681 caccacgcac atcagcgtgt gacggacatt gtcgaatatt atcatttcat ccggcatcat
     7741 gaattcagcc atgacggcgt tttcgtcgag gcgattcggg atattttcaa aaaagctcac
     7801 cgtttcatac gtaaaatacc cgaccatgcc gccctggaag cgtggcagtt cagggctcag
     7861 ggccaccttg aagcggctca tgaattcacg cagcaccgcc agagggtctc cgccgtgcgg
     7921 tatgacttcc cgctgtacgc cgttgtgtat ttccactcgg tcgcgataga caagaaccct
     7981 ggcacgcgac gaaaggccca aaaagctgta tcggccccat ctggctgtgc cttccatgct
     8041 ttcaaacagg aaacaatccc tgcccgggca gaaaaatttg cgtagcagcg acacgggcgt
     8101 atccatatca gccaatattt cggaacagac cgccacgaca ttgtgttccg aggcaagttt
     8161 ttcgtagcct ttcctgtcgg gaaaacttct tataaacatt tgtatctcca ataaaaaagg
     8221 ttgtgaactt caatcacggt tcacaacctt gttaaatatc cccattcgga atattttctg
     8281 gctatgaacc ttttacacgg ctcacaaccc actaaaacat ccaactcgga catccgggcc
     8341 gtgaaccggc ttcatcgccg ggcccggagt gaatgcgtac agtcaccctc aacgcccggc
     8401 cttcggccag cgccaccacc aacgccggat tgtcctgtcg atgttacgca tcatatcgat
     8461 ttcataagct ctatcatttc tttcctgtcg tttttgtcaa gcgcattttt caatctttcg
     8521 acagccgccg caaattcgtc aaaaacttca cggctatgcg ggttgttcag ggcgatatcg
     8581 gcaaagagtt ccgccgactg ggacgcctgc cggtcgcgca attccttcag ccgcccgaac
     8641 cagtcgccga cattgaccag ctgaccggcg tcgccgaatt tggcaatcgt cgctccgaag
     8701 ccagccatca tcatgtgcct cagcgactgg acaatactca tctgccggtc atgctccgcg
     8761 gcggtaagga ctattatacg aaatttcagt ctttttagca ggcttttgaa tcgcgtcgta
     8821 aggttgccca gccgcgccgg acaaagaaag aaaatctgtc cttcaagatt tttttccgcc
     8881 ggcccgaaca gcggatgccc gccgagcacc tcgcccctgc aatggttgag catgatttta
     8941 acgggcagtt ccttaaccga cgttatatcg gcaacaaggc cgtctttcct ggtatgaggg
     9001 ccaagtttga ctactacctc ctcaaagcag cgaatcggca cagccagcac aaccacatcg
     9061 gcggcagctg cggcggcgta atccatctca ccgtcggagg aatcgaacga tctgacggtc
     9121 aggccctgcg cgcggaacat gtccacgaaa cgccggccca tcgcgccgcg tccgccgata
     9181 acactgattg tgcgtatcat ctgaaccatt atctcttttc accagccatg aaatcttcgg
     9241 atgtcgggta cgaacccatg acgtaaatct ggccgaccat gtcggaaagc gtcgtaagcg
     9301 acttagccac cggctcatca ctgcgatggc cttctatatc caggaagaac acatattccc
     9361 aaacgtgcga cggcgaaggc cgtgattcga ttcgcgttat gtttacgccg ttttcggcaa
     9421 acacgcccaa acagcgaaac agcgagccgg gcttgtgcga ggcggcaaac cgcagcgacg
     9481 tcttgtcgtg gccggttgga tagcagcttt cccttgaaat gacccagaac cgcgtctggt
     9541 tggagcttct gtcctgtata tcgctcgcca gggctgtcaa gccgtatttt tcgcgtacca
     9601 gttcgccgcc gatggcagcg gcatccggtt cggccgccgc gcgttcgccg gctttagccg
     9661 tgctttcgag cgcgacacag acggcattgg gcagatattt agtcagccaa ttccgacact
     9721 gggcaatcgg ctgaggatgc gagtagactc gcctgatatc gccaagcgac gcagccttcg
     9781 acaacagcga attggatatc cgcagacaca tttcgccgca tatctgcaac ggcgaatgca
     9841 ggaacgcatc gaacgtttcg ccaaccgtgc cgttggtgga gttctcaacc ggcactacgc
     9901 cgaattccac attgccggac tcgaccttgc gaaagacgtc ttctatgctc tccgacggaa
     9961 caaagcaggc gcactcgcca aaacgcctca tcgcagcaag gtggctgaag gtggcttcgg
    10021 ggccgaggaa tgccacgcgg acgttagcct gcaaaccgcg gcaggcactg attatttcac
    10081 gaaagatgtg ctcaatggaa tccggcacaa gagggccgtg aatgctgctc ttcagccgcc
    10141 gcataacctc acgctcgcgg gcagggtcac acacttccat gccgacaacg cgcttcgtct
    10201 ggccaatggc aagcgcaact tcagcccgct tctgcagcag ttccagcagt tcgttatcca
    10261 gttcgtttat ctgctttctc aggttttcaa gttcgccggc catgcatgtc ctttcgttag
    10321 cgtcacatcc gcatatccat gacgtcagcc atccgcgaga gcaccttcat cagttcgccg
    10381 aaagcgtcgg gcgttatcga ctgatcgccg tcgcaaagtg cctggcaggg attgttgtgc
    10441 acctctatca tcagaccgtc cgctccgact gcaagactcg ccttagccag cggcgcgacg
    10501 aattcactta tgccgaccgc atggctgggg tcaactatca ccggcagatg cgacttcttc
    10561 tttataaccg gaatcgccga caaatcaagt gtgttgcggg tagccgtctc gaacgtgcg
//
LOCUS       DAONFI010000029        10608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7092_length_10608_cov_4.489719, whole genome shotgun sequence.
ACCESSION   DAONFI010000029 DAONFI010000000
VERSION     DAONFI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7092_length_10608_cov_4.489719"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLK08_01705"
     CDS             <1..350
                     /locus_tag="PLK08_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HPS52242.1"
                     /translation="LKPCTQFGFRNTLLSLIEVYEDAAAHGECEMKFAGMAKIDGHDC
                     LVIERHLPYRPEYPAMKTVVYIELDNLLPLEIWGYDWKGELTSHYAYHDVNLAADLSI
                     NDFSPKANGIKGH"
     gene            complement(354..1958)
                     /locus_tag="PLK08_01710"
     CDS             complement(354..1958)
                     /locus_tag="PLK08_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52243.1"
                     /translation="MMRYLGTILVLNLCLASLAWPDAIAFFREKQAPTGGWDVSPALY
                     ADGAGDGLIVWAALVNGVSPKSPWLHRATDNLMAARPKTVFSRAMRIMAMTADAAAAD
                     RKIISEDVAWLSDLQSPDGGWPAGRDSSPDKLDTLDTAWAVLALDRAQSVGGVAVNKA
                     VFQRAVKFFQTAVNDDDGYGYDWPKARPDRLRGQSHSLTTAAAIAALDVLNKYVGGQD
                     EDADMPESLADSARGWLSGKDSPMKIENWYWGSQPLGQYRYFLARATVDRQAVTDAIC
                     SVQDKNKCTPANGLAEGRLMSTAWAILTLAENSSPKTAALATTNANMKNSDVSLPRTY
                     IPKLVREIYVASWSPRDNYPSRASEVLADELRDAYSLGLECIALDSPDKLEGQGARLL
                     WCTGFPAEIDIKYQSQLLRFIADGGVVLLDSADDVEYDSAASVLRKMFGDAAFYPVTA
                     SMPLATGNFGGGVGLDVTDKLGCEKKLSRPLLMAVVKNGRTVAVLSAVPLAKIASGNF
                     VQDNPPMQSSDAKEILLNIILFADTQ"
     gene            complement(1955..3325)
                     /gene="der"
                     /locus_tag="PLK08_01715"
     CDS             complement(1955..3325)
                     /gene="der"
                     /locus_tag="PLK08_01715"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPS52244.1"
                     /translation="MSLLPIVSIIGRPNVGKSSLFNRLAGRMLSIVADRPGVTRDRLS
                     APVALGDGEKARYVELIDTGGMGIEDSDNLTDDVEFQIRSGIDMSDVIIFLVDAREGI
                     VPLDSLVAQKLRRSNKPVIFAANKVDEANLTSEIGEMGRLGFGEPIALSATHGRGVRD
                     LLDRIERELKDVPPVRPAEAVMQLAIVGKRNAGKSTLINALCGEKRVIVSEVPGTTRD
                     SVDVRLCFNGKEILLIDTAGVKRKKKLADEIEFYSQHRALRSVRRADVTAMVIDASVP
                     VSQIDKDLAGTIAAEFKPVLIVVNKWDLAKNAASTDDYAEYFGKTFPELSFAPIIFTT
                     ATQGMNVKTVLDISSELCAQSRHRVTTGQLNAAIEAITSKRGPSHKTGTKPPKIFYSS
                     QIDIEPPTIVMFVNDARSFDKVYQRFLVGRLREYLAFSEVPIRLMLRGKETYRAYMDK
                     KNRPRE"
     gene            complement(3416..3859)
                     /locus_tag="PLK08_01720"
     CDS             complement(3416..3859)
                     /locus_tag="PLK08_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPS52245.1"
                     /translation="MNDRFMNEAIIEARRGLADGGIPVGAVLVYHDEIIGRGHNRRVQ
                     RSNPILHATIAAVENAGCQPAGVYHASTLYTTVSPCMMCSGTIMLYGIGGVVIGENRS
                     FQGEEELLRQRGVKVTVLQDRDCIRMMDEFINNNPHLWLENIGRK"
     gene            complement(3929..4234)
                     /locus_tag="PLK08_01725"
     CDS             complement(3929..4234)
                     /locus_tag="PLK08_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPS52246.1"
                     /translation="MPTYDYECSECGHRFEKFQGMTAKPIKKCPKCGKMKLRRLIGIG
                     AGVIFKGSGFYQTDYRSSGYREAARKDTSSSSASASSAGGSKTTVNSDKTDKKASKK"
     gene            complement(4499..6505)
                     /gene="tkt"
                     /locus_tag="PLK08_01730"
     CDS             complement(4499..6505)
                     /gene="tkt"
                     /locus_tag="PLK08_01730"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015221841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPS52247.1"
                     /translation="MATHIGKAIDPELSQKIATTIRFLAADGVQKAKSGHPGMPMGCA
                     DIATVLLTRVLRIDPSDTNWFNRDRFVLSAGHGSMLIYSMLHLAGVLSMDDLKQFRQY
                     GSKTPGHPEVHDVPGVDVTGGPLAAGFATSAGLALAERMLAQRYSDDVIDHFTYVIMG
                     DGCHMEGLSQEAASFAGHLKLGKIIAFYDDNEISIEGSTNIAFTENVNKRYEALGWHV
                     QDIDGHNHDAIAAAVEEAQNDPRPSLIVCHTVIGRGAPKKQGTASSHGEPLGEEEILA
                     AKKAIGWSEETFYVPCEVYDYFKTRRAEWAAIRKEWDAKLAGLEKSEPKAAAEIKRII
                     AGEMPANWEKARPTFPADPKGMASRASSGKVLNAFAAVIDELVGGSADLAPSNKSEMT
                     AGKWNGYVSAGEYFNRNIHFGVREHAMGNIVNGLALHGLIPFGATFLVFHDYMRAAVR
                     LSSIMHCRSIWLYTHDSFYVGEDGPTHQPVEQLASLRAIPRLHLMRPADANEVAYAWQ
                     HALSRKDAPTAFAMTRQDLPTLDREKYACACGTLKGGYVLSDDENAEIMFIATGSEVS
                     LALSVAEELRTQGRKVRIVSMPCLDLFREQPADYQAAVIPASITRRVVLEAGIRQGWE
                     GYLGSDGIFVGKEDFGTSGPYKVLAEKFGFTKEAVLAKIKAAKF"
     gene            6802..7368
                     /locus_tag="PLK08_01735"
     CDS             6802..7368
                     /locus_tag="PLK08_01735"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPS52248.1"
                     /translation="MHSKEVLGEGKYYRMVKAKGWEYVEPVGFSDVVLIIPVTDDGRM
                     VLIEQYRVPADKQVFEIPAGLVGDKEHIKDENIIEAARRELIEETGYDAKNITIAIKA
                     APNAGSNTLEVHMLLATGLKKVGNGGGNPEEGENIKVYEVPLAHVRTWLNDKIAHGHV
                     VDLKVYAGLFFAMEYWNEHQNALDGHNR"
     gene            7833..8486
                     /locus_tag="PLK08_01740"
     CDS             7833..8486
                     /locus_tag="PLK08_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52249.1"
                     /translation="MKIIIILIACSVVIIAPVRAEYQSFTYGGHTYRLYFGKDMRTWD
                     DARKFAASTSIGIARGYLSNVNSAAENAAIYSVIHKVFDKAGYLGGIGSAARDGGGAC
                     YAWLGGNDMASEGEFYWASSTKISAQKFWTGGHAGHAVDSMYVNWGAGKLGHEPDNYN
                     SSQNAVAIAAEKWPLRGGIGCGGQWNDINQNNVISFVVEYDTQTPAGKPTEKMPPKN"
     gene            complement(8476..9246)
                     /locus_tag="PLK08_01745"
     CDS             complement(8476..9246)
                     /locus_tag="PLK08_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPS52250.1"
                     /translation="MRIAGIEKSSFVDYPGRLATVLFTPGCNLDCFYCHNRHLLAALD
                     EEKFYAPQDILNFLRSRQGLIDAVVISGGEPTLQNDLFEFMYQIRQMRFEIKLDTNGL
                     RPWIVKSLITHKLVDFIAMDIKAPLHKYFRIVNSDVPQEILRESIDLIMSSNVEYEFR
                     TTVAPMLTMSDILDIATMIKGAKRYALQQYRQPGTAVDMFGVVRYPDPHPVSFLHQAH
                     ELIAKFVQHCEIRGLGGDQPKPVTLAYEPSAIDEPVNF"
     gene            complement(9331..>10608)
                     /locus_tag="PLK08_01750"
     CDS             complement(9331..>10608)
                     /locus_tag="PLK08_01750"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPS52251.1"
                     /translation="NLTFDLKPPATLRDQAVIVGGEIKPDKYGDFQAEMDIFNKAFCE
                     VMMEGDSKGRVFTFPVPTINVTRDFDWDSPSVNSFMDATAKYGIPYFANYVNSDLDPE
                     DAVSMCCRLRLDTRELRKRGGGLFGSNPMTGSIGVFTINLPRIGYTTKTKEAFLARVT
                     ELMHVGKTQLEIKRRIVEEQTENGLYPYSQFYLRNIKLRTGEYWTNHFNTIGLIGMNE
                     AMLNFMGCGVVSQKGLEFALEIMQHMRDELAKIQEETGHIYNLEATPAEGTSYRLAKL
                     DRERYPEIITAGDEVPYYTNSSQPPVGYTEDIFEVLENQDELQSMYTGGTVLHLYLGQ
                     KVESPELCKRLIQKIFKKYKLPYISITPTFSICNQHGYIAGEHYHCPYCDSKTEVWSR
                     VTGYLRPIENYNKGKKAEYLERKKYVMPKLVEV"
BASE COUNT         2593 a         2862 c         2603 g         2550 t
ORIGIN      
        1 cactcaagcc gtgcacgcag ttcggcttcc gaaacacact gctaagcctt attgaagtat
       61 acgaagacgc cgcagcccac ggcgaatgtg aaatgaaatt tgcaggcatg gcaaaaatcg
      121 acggccatga ttgtctggta atcgaacgcc acctgcccta tcgccctgaa tatccggcga
      181 tgaagaccgt cgtctacatc gaattggaca accttctgcc gcttgagata tggggttatg
      241 actggaaagg cgaactcacc agccattatg cataccatga tgtaaatctg gcagccgacc
      301 tcagcatcaa tgacttctcg ccaaaagcca acggcattaa aggacattga tttttattgc
      361 gtatcggcaa acaggataat attaagcaga atttctttag cgtcggacga ttgcattggg
      421 ggattatctt gaacgaaatt gcccgacgct attttcgcca gcggcacagc cgacagaacc
      481 gccactgttc tgccgttttt aaccaccgcc atcagcagcg gccgtgacag ttttttctcg
      541 cagccgagct tatccgtaac gtcaagcccc acgccgccgc caaaattgcc cgtcgccagc
      601 ggcatagacg cagtcacagg ataaaacgct gcatcgccga acatctttcg caaaacggat
      661 gcggcagagt catattccac atcgtcagcc gaatccagca acaccacgcc gccgtccgct
      721 atgaatcgca gtaattgcga ctgatattta atatctattt cagccggaaa gccggtacac
      781 cataaaagcc gagcaccctg gccttcgagc ttatcgggcg aatccaacgc aatacactcc
      841 agccccagac tgtacgcatc gcggagttcg tcagccagca cttcagaggc acgcgacgga
      901 taattatcgc gcggcgacca cgatgcgacg taaatttccc tgaccagttt cggaatataa
      961 gtccgcggca gactgacgtc gctatttttc atattcgcat tcgttgtggc gagggcagca
     1021 gtctttggcg atgaattttc cgccagcgtc agtatcgccc aggcagtcga catcagcctg
     1081 ccctccgcca atccgttagc cggcgtgcat ttattcttgt cctgaacgga gcatatcgca
     1141 tccgtcacag cctgcctgtc aacggttgcc ctggccagaa aataacgata ctggcccagc
     1201 ggctgagaac cccagtacca gttttcaatt ttcatgggcg aatctttgcc gctcagccag
     1261 ccacgggcac tgtccgccaa tgattccggc atatcggcgt cttcatcctg cccgccgaca
     1321 tatttgttca aaacgtccag agcagctatc gcggcagccg tagtcagcga gtgcgactgg
     1381 ccacgcagcc ggtcaggccg tgcctttggc caatcataac cgtagccgtc gtcatcattc
     1441 acggcagtct ggaagaattt tacggctcgt tgaaaaaccg ctttattcac agctacgccg
     1501 cccacgcttt gagccctgtc cagagcaagc actgcccacg ccgtatccag cgtatcaagt
     1561 ttgtcaggcg aagaatcgcg accagcaggc catccgccgt cgggcgactg caaatcgctc
     1621 agccacgcga cgtcttcaga aatgattttt ctatccgccg ccgcagcgtc agccgtcata
     1681 gccattattc gcatcgcgcg gctgaacacg gtcttcggcc tggcagccat aagattatcc
     1741 gtcgcacgat gcagccatgg agatttgggc gaaacgccgt tgactaatgc agcccatact
     1801 atcaggccat cgccagcccc gtcggcatac aacgcaggcg aaacgtccca tccacccgtc
     1861 ggagcctgtt tttcgcggaa aaacgcgatt gcatccggcc aggcaaggct ggccagacag
     1921 agatttaata ccagaattgt ccccaaatac ctcatcattc ccggggcctg tttttcttgt
     1981 ccatataagc ccgatacgtc tccttcccgc gaagcatcaa acgtatcggc acttctgaaa
     2041 acgccaggta ctcacgcagc cggccgacca gaaaacgctg ataaaccttg tcgaaactgc
     2101 gagcgtcgtt cacgaacatc acgattgtcg gcggttcgat atctatctgc gacgaataga
     2161 atatcttggg cggttttgtg ccggttttgt gacttgggcc gcgtttggac gttatcgcct
     2221 cgatagctgc gttaagctgg ccagtcgtca cgcgatgtcg agattgcgca cagagttcag
     2281 aagaaatatc gagaaccgtc ttgacgttca tgccttgcgt agccgtcgta aaaattatcg
     2341 gagcgaacga aagttcagga aaagttttgc cgaaatattc ggcataatcg tccgtggaag
     2401 cagcattttt cgccaaatcc cacttgttca cgactataag aacaggtttg aattccgcgg
     2461 caatcgtgcc ggcgaggtct ttgtctatct gggacaccgg cacggaggcg tctatcacca
     2521 tcgccgtcac gtccgcgcgg cgaactgaac gcaacgccct gtgctgtgaa taaaattcga
     2581 tctcatccgc gagttttttc tttcgtttca caccggcggt atcgatgagc agtatttctt
     2641 tcccattgaa gcagagccgc acatccacgc tgtcgcgggt agtgcccggc acttcggaaa
     2701 cgataacccg tttttcgccg cacaaagcgt ttatgagcgt acttttgccc gcgttgcgtt
     2761 tgccgacgat agccagctgc attacagcct ccgccggccg aactggcggc acgtccttca
     2821 attcacgctc tattctgtcc aacaggtctc ttacgccgcg gccatgagtc gcactgagag
     2881 ctatgggctc accaaaaccc aggcgaccca tctcgccgat ttcgctcgta aggttcgctt
     2941 cgtcaacctt gttggctgcg aatatgacag gtttattgct ccgacgaagt ttttgggcta
     3001 ccaggctgtc cagcggaaca attccttcgc gggcatcgac caggaaaata atcacatccg
     3061 acatatcaat gccggaacga atctgaaact ccacgtcgtc cgtaaggtta tcactatctt
     3121 caatgcccat gccgcccgta tcaatcagtt cgacgtagcg ggctttttcg ccatcgccca
     3181 gcgctaccgg cgcacttaac cggtctcgcg tcacgccggg tctgtcagcc acaatggaca
     3241 gcattcggcc ggccagccgg ttgaaaagac tgctcttgcc cacattgggc cggcctatga
     3301 ttgatactat gggtaataag gacatgtttt ctttctttgt gtatctgttt cgccgaaatt
     3361 ttcaatatgc aaaaatcgta tgcgctactt caaaccgtca gccggcaaag cctcgctatt
     3421 tcctgccgat attttccagc cacagatgcg gattattgtt tatgaattcg tccatcatcc
     3481 ggatgcagtc cctgtcctgc agaacggtca cctttacgcc gcgctgccgc agaagttctt
     3541 cttcgccctg gaaagacctg ttttcgccta tcaccacgcc gccgatgccg taaagcatta
     3601 tcgtgccgct gcacatcatg cacggcgaga cggtggtata gagtgtactt gcgtgatata
     3661 cgcctgccgg ctgacagccg gcgttttcta cggcggcaat cgtggcgtga agtatcggat
     3721 tgctgcgctg tacgcgccgg ttatggcctc tgccgattat ttcgtcatga taaacaagca
     3781 ctgcaccaac aggtatgccg ccgtcggcaa ggcctcgcct ggcttcgatg atggcctcgt
     3841 tcatgaatct gtcgttcata ttccactcct aatcgggggt gtgcaaatcc acgatatttg
     3901 tccagccatc aaatttacct tatagcggtt attttttcga ggcttttttg tcggttttgt
     3961 cgctatttac agtcgttttg cttccgccgg ctgaggatgc ggaagcagat gaggatgagg
     4021 tatcttttct ggcggcttca cggtagccgc tggagcggta gtccgtctga taaaagccgc
     4081 tgcccttgaa tatcacacca gcaccaatcc cgatgagcct tctaagcttc atttttccac
     4141 atttgggaca tttctttatg ggcttcgcag tcatgccctg aaacttctca aaacggtggc
     4201 cgcattcact gcattcgtaa tcataagtag gcatttcagt aatctcgtaa aaccgtgtat
     4261 tttaacacat aaagctaaaa tattcacgtt aaattcatca cgttaaaaaa tgttcaaagc
     4321 ccggccatcg aatgaccggg ctttgaatgg agtgcattaa atttctacgg gtcaaaaccc
     4381 gtgcatcaca cttatcaggc tatcgcctgc ctgtccgcct gacggcagat gtttttatct
     4441 acagtttttt aaaaaaccgt agaatcacaa aacgacatat tttaaaaaaa ccttgcgatt
     4501 agaatttggc ggcttttatc ttcgccagaa cagcttcttt tgtaaagccg aacttttccg
     4561 ccaacacctt gtacggcccg ctagtgccga aatcttcctt gccgacgaat atgccgtcgc
     4621 ttcccagata gccttcccag ccctgccgga tacccgcttc gagcacgacc cgcctggtga
     4681 tggatgccgg aatgacggca gcctggtaat cggccggctg ttcacggaaa aggtccagac
     4741 acggcatgga gacaatgcgt accttgcggc cctgcgtgcg gagttcttcc gccacgctca
     4801 gagccagcga aacttcactg ccggtggcaa taaacatgat ttccgcgttt tcatcgtcac
     4861 tcagcacata accgcccttt agagtgccgc acgcacatgc gtatttttcg cggtcgagcg
     4921 tgggcaaatc ctggcgagtc atcgcaaagg cagtaggggc atccttgcga gacagcgcat
     4981 gctgccaggc gtaggcaact tcgttggcgt cagccggccg cataaggtgc agccgcggaa
     5041 tagctctcaa cgaggcaagc tgttcgaccg gctgatgagt cgggccgtct tcgccgacgt
     5101 agaagctgtc atgcgtgtac agccatatgc tgcggcagtg catgatgctg gacagacgaa
     5161 cggcggcacg catgtagtcg tggaacacaa ggaacgtcgc accaaacggt atcaggccgt
     5221 gcagcgccaa gccgttgact atgtttccca tagcgtgttc gcgaacgccg aaatgtatgt
     5281 tgcgattgaa atattcgccc gccgaaacat agccgttcca tttgccggca gtcatttccg
     5341 acttgttcga cggagcaagg tcggcagaac cgcccacgag ttcgtcgata accgcggcga
     5401 atgcgttgag cactttgccg ctcgacgcgc ggctggccat gcctttgggg tcagccggga
     5461 aagtcggccg tgctttttcc caatttgccg gcatttcacc ggcgatgatg cgcttgattt
     5521 cggcagcagc cttcggctcg gatttttcca agccggctaa cttggcatcc cattccttgc
     5581 gaatggcggc ccattccgcg cggcgggtct tgaaataatc gtaaacttca cacggcacgt
     5641 agaatgtctc ttccgaccag ccgatagcct tcttggcggc gaggatttcc tcttcgccga
     5701 gcggctcgcc gtggctggag gcagtaccct gctttttcgg ggcgccgcgg ccaatgacgg
     5761 tgtggcagac tatcaggctc ggccgcgggt cattctgtgc ctcttcaacc gcggcggcaa
     5821 tcgcgtcgtg attgtggccg tctatatcct gaacatgcca gcctagggct tcatatcgct
     5881 tgttgacatt ttcggtaaac gctatgttcg tgctgccttc gatgcttatt tcattgtcgt
     5941 cgtaaaaggc aataatcttg cccagcttga gatggccggc aaaagaggcg gcttcctggg
     6001 aaagcccttc catatggcag ccgtcgccca tgatgacgta ggtgaaatga tcaatcacgt
     6061 cgtcgctgta acgctgtgcc agcatacgct cggcaagagc aaggccagct gacgtggcaa
     6121 aacccgcagc cagcggccca ccagtcacgt cgacacccgg cacgtcatgc acttcaggat
     6181 ggccaggcgt cttcgagcca tactgacgga actgcttgag gtcatccata gacaaaacac
     6241 cagccaggtg aagcatggaa tatatcagca tactgccgtg gccggcggag agaacaaacc
     6301 ggtcacgatt aaaccagttg gtatcggacg gatctattcg aaggacgcgg gttaaaagca
     6361 cggtcgcgat atccgcacag cccatcggca tgccggggtg gccggatttg gctttctgta
     6421 cgccatcggc ggcaagaaag cggatagtgg tcgctatctt ctggctcagc tcagggtcga
     6481 tagcctttcc aatatgtgtt gccatcattc tttcctcata aaaagcattt tcttgacaaa
     6541 atttcgcagg aaactttggt ttttcacatt tttcattcgg ggtacagcct atgtcgccga
     6601 caaaatccat taaaaaaatc acagccagcg acaattcatt ggagatggat tataaaaatc
     6661 acattgtttt acggctgtac ggccatattg aggctgatta ctcttgaaac cagacatcaa
     6721 gcggggtatt ttaataataa tggcgtaaaa atacaagttt ttcgccctgc cgtcagcaag
     6781 aattcaatgg agtgataata aatgcatagc aaggaagtat tgggcgaggg taaatattat
     6841 cgtatggtca aggctaaagg ctgggagtat gtagagccgg tggggttttc cgacgtcgtt
     6901 ttgattatcc ccgtaaccga cgacggcagg atggtactta tcgagcaata ccgtgtgccg
     6961 gctgacaagc aggtttttga aataccggca gggttggtag gcgacaagga acacatcaaa
     7021 gacgaaaaca taatagaggc ggctcgtcgc gaacttatcg aggaaaccgg ctacgacgcc
     7081 aaaaacatca ccattgcgat aaaagctgcc cccaacgccg gctcaaacac cctggaagtg
     7141 catatgcttc tcgcgaccgg cttgaaaaaa gtcggcaacg gcggcggcaa tccggaagaa
     7201 ggcgagaaca ttaaagttta cgaagtgccg ctagcccatg ttcgaacatg gctgaatgac
     7261 aagatagccc acggccatgt cgtcgacctg aaagtctacg ccggcctttt cttcgcaatg
     7321 gaatactgga acgaacatca aaatgctctt gatggacaca atcgttaata atgccacttc
     7381 gttgaatatt taaagaaaaa tgtgaatctg aacgtacatg aaccgctgtt taagatggag
     7441 cgtgaaatcg ctcttaatta agagcttcta acaaaatgag atgtcgcacc gaggcgtagt
     7501 tggttgattg gctggcaagg aaggcgaagc agatagcctt atagggcttg cgatgcagcc
     7561 tgacgccgcc agacattcaa ccaacaagcc gaatgcaata tcattttgtt agaagctcta
     7621 aacgacctaa aactatcact tctaaaaaga cgtacgatta ccgtgcaaaa ccgccaaaag
     7681 gatattctct tttttgatat aatcttagtg attctagaac ctatccccaa aacatgggcg
     7741 ttgcctaaat cgagcaattt tgcggaacag tcccaaaaac ctccccagac tgctataatt
     7801 tatattataa aatgttttat gaaggtgcga atatgaaaat aatcatcatc ctgattgcgt
     7861 gcagtgtcgt aattattgcg ccggttcggg cagaatatca atcgttcaca tacggcggcc
     7921 atacatacag gctgtatttc ggaaaagaca tgcggacatg ggacgacgcc agaaaatttg
     7981 cggcatccac ttccataggc atcgccagag gttatttgtc aaacgtgaat tcagccgctg
     8041 aaaatgccgc catatattct gtaatacaca aagtatttga caaggctggt tatctcggcg
     8101 gcatcggcag tgccgcacga gacggcggcg gagcctgtta tgcttggctg ggcggcaacg
     8161 acatggccag cgaaggtgaa ttctactggg cgagttccac taaaatatct gctcaaaaat
     8221 tctggacggg cggccacgcc ggccatgctg tagattcgat gtacgtcaac tggggcgccg
     8281 gcaaattggg acatgagccc gacaattaca actcatctca aaacgcagta gcaatcgcgg
     8341 ctgaaaaatg gccgcttaga ggaggaatcg gctgcggcgg ccaatggaac gacatcaatc
     8401 agaacaacgt catctctttc gtcgtcgaat atgacacgca aacaccggcc ggaaaaccaa
     8461 ctgaaaaaat gccacctaaa aattgaccgg ctcgtctatg gctgaaggtt catacgccaa
     8521 cgtcaccggt ttgggctgat cgccgccaag gccacgaatt tcgcaatgct gaacaaattt
     8581 ggctataagc tcatgcgcct gatgcagaaa actcaccgga tgcgggtcag gatatctcac
     8641 cactccaaac atatccacgg ctgttcctgg ctgacgatat tgctgaagag cgtagcgctt
     8701 agcgcctttt atcatagtgg caatatccag aatgtccgac atggtaagca tgggggctac
     8761 cgtcgtgcgg aattcatatt cgacattgct ggacattatc aggtctatgc tttcccgcag
     8821 aatttcctgc ggcacatcac tgttgactat gcgaaaatat ttatgcagcg gcgcttttat
     8881 gtccatcgct atgaaatcga caagtttgtg cgtaatgagc gatttgacaa tccagggccg
     8941 caaaccgttg gtatccagct tgatttcaaa acgcatctga cgaatctgat acatgaattc
     9001 gaacaggtcg ttttgaagag tgggctcgcc gccggatatc acaactgcgt caataagccc
     9061 ctgccgcgac ctgagaaaat tcagaatatc ctgcggagcg taaaattttt cttcgtcaag
     9121 agcagccagc aaatgccggt tatgacagta aaaacagtca agattacagc ccggcgtaaa
     9181 cagcaccgtc gccagcctgc caggataatc cacaaacgaa cttttttcta tccctgctat
     9241 tctcatatat cattcacata acggctataa acaataataa aactcacgga aaccatatac
     9301 ccctactacc gcatccggct gccgaattca tcacacttca accagcttgg gcattacata
     9361 ttttttacgt tcaagatact ctgctttttt gcccttgtta taattttcta tcggccggag
     9421 atagcccgta acgcgcgacc agacctcggt ttttgaatcg caatacggac aatggtaatg
     9481 ctcgcccgca atatagccgt gctggttgca tatgctgaac gtaggcgtaa tcgatatata
     9541 cggcagtttg tattttttga agattttctg tatcagccgt ttgcagagct caggactctc
     9601 aactttctgg ccgagataca gatgcagcac cgttccgccg gtatacatcg actgcaattc
     9661 atcctgattt tccagaactt caaaaatatc ttccgtatag ccgaccggcg gctgagacga
     9721 atttgtatag taaggcactt catcgccggc agttatgatt tcaggatatc gttccctgtc
     9781 tagcttggcc aggcgatagc tcgtaccttc ggcgggagtg gcttcaaggt tataaatatg
     9841 gccggtctct tcctgtatct tcgccagctc atcgcgcatg tgctgcatga tttccagcgc
     9901 gaactccaaa cccttctggc tcacgacgcc gcagcccata aaattgagca tcgcttcgtt
     9961 catgccaatc aagccgatgg tgttgaaatg attcgtccag tattcgccgg tacggagctt
    10021 gatgttcctc aggtagaact gcgaatacgg ataaaggccg ttctccgtct gctcctcgac
    10081 gatacgccgc ttgatttcga gttgcgtttt gccgacgtgc ataagctcgg tcacgcgagc
    10141 cagaaatgcc tcttttgttt tcgtcgtata acctatgcgc ggcagattga tggtaaaaac
    10201 gccgatagaa cccgtcatgg gattgctgcc gaaaaggccg ccgccgcgtt tgcgcaattc
    10261 gcgggtatcc aaacgcagcc ggcaacacat gctgacggcg tcttcagggt caaggtcgga
    10321 attgacgtaa ttggcaaagt acggaatgcc gtatttggcc gtcgcgtcca taaatgaatt
    10381 gaccgacggc gaatcccagt cgaaatcgcg cgtgacgttt atcgtcggca cggggaaggt
    10441 aaagaccctg cctttggaat cgccttccat catgacttcg cagaaggctt tgttgaaaat
    10501 gtccatctca gcctggaaat cgccgtattt gtccggtttt atctctccgc cgacgattac
    10561 cgcctggtcg cgaagagtcg ccggcggctt caggtcaaac gtaagatt
//
LOCUS       DAONFI010000030        10577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7118_length_10577_cov_4.328360, whole genome shotgun sequence.
ACCESSION   DAONFI010000030 DAONFI010000000
VERSION     DAONFI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10577
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7118_length_10577_cov_4.328360"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..2711
                     /gene="clpB"
                     /locus_tag="PLK08_01755"
     CDS             126..2711
                     /gene="clpB"
                     /locus_tag="PLK08_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPS52252.1"
                     /translation="MRMDKLTVKAQEAFMGAQGRASEAGNPQITPLHLLAEMAEQEGG
                     VVGPLLEKVGISHNRLMTVINAELDRLPKQSSGGGMGMDSTLNNVMTQAGKEAKALKD
                     DYISVEHFLLAMTQEESNARDVLLTLGATHDEVLKAMQEIRGSQRVTDQNPEEKYQAL
                     KKYGRDLCEIARSGKLDPVIGRDEEIRRCMQVLSRRTKNNPVLIGLPGVGKTAIVEGL
                     AQRIVNGDVPNVLLNKRVIALDMGALLAGAKFRGEFEDRLKAVLNEVTQAQGEIILFI
                     DELHTVVGAGAAEGAISAGNLIKPALARGELRCIGATTLDEYRKHVEKDAALERRFQP
                     VLVDEPSVEDTIAILRGLKPRYDAHHGVRIQDAALVTAAVMSHRYIADRQLPDKAIDL
                     IDEAASRLRIENDSMPSELDELNRRIMQLQIEREALRKESDDASKKRLVELEKQLADM
                     QSTFDGMKAEWENETSLLSNTKKLREAIEDKKAQLDQAQRTGDLETAARIQYGELRDL
                     QTKLSNAEKAIVEKKEHGSLTSEEVTPDQIAEVVAKWTHIPVSKLMESERDKLLTMED
                     QLRLRIVGQDDALRAVADAIRRNRAGLGDPHRPIGSFLFLGPTGVGKTELCKALAEML
                     FDTEDAMVRIDMSEFMEQHSVARLIGAPPGYVGYEEGGRLTEAVRRKPYCVILFDEVE
                     KAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTIIVMTSNIASQYIQSLAEKHAEDWEI
                     EAQIKQTLRDYFRPEFLNRIDERVIFHALGKEELEGIVEIQVRQLAKRLADKNVSLRI
                     SPAAKDLLCEFGYDPAFGARPLKRVIQQYLENELAKKILTGEVMAGQTVVVDAQGKAF
                     HFSVE"
     gene            complement(2975..4819)
                     /gene="mnmG"
                     /locus_tag="PLK08_01760"
     CDS             complement(2975..4819)
                     /gene="mnmG"
                     /locus_tag="PLK08_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPS52253.1"
                     /translation="MEFDIVIVGGGHAGIEAVWAAARMGANAALVTMYKNTIGQMSCN
                     PAIGGIGKGQIVREIDALGGLMGLAADANGIQFRMLNASKGPAVQGPRCQADRHAYSG
                     WMQEKLLTTPGIMILEGMAIRICTSGGKVCGLTYRPVNADADVTLKCSAVIVTAGTFL
                     AGIMHRGPEKWAGGRINEPAANELSESLRNCGLTLGRLKTGTCPRLAAETVDYSRCTR
                     QDGDAVPSPFSFMNRQLDVEQIPCWLTATNEKIHQAIRDNFHRAPMYTGQITSVGPRY
                     CPSFETKVQRFADKTSHQVFLEPEGRNTNWIYCNGITTSMPRDVQDFMVANIPGLENA
                     KILQYGYAIEYDFAQPTQLFSTLETKCMQGLYLAGQLNGTTGYEEAGALGMMAAINAA
                     LKLQNRAPLILTRQQAYMGVMIDDLITKGVTEPYRMFTSRAEHRLTLRYDNADRRLTP
                     VGCDIGTVDTERHKVFEEKSRNCSAAENIMHTTRIEGKTLWELMRRPGAKIDLLINEA
                     GESGKPLQELQNLDAQAVNTIAIDAAYEGYTVKQEQYNARFKDLDAKKLPAGFDYFSV
                     KQLRWEAKEKLSKIQPANLGQALRVSGITPADITVLAISMADSHNSGS"
     gene            complement(4882..5709)
                     /locus_tag="PLK08_01765"
     CDS             complement(4882..5709)
                     /locus_tag="PLK08_01765"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPS52254.1"
                     /translation="MQKNCNYNLQEHEPDILLDAGKILDGLDTLRVGRSILCFKEVNS
                     TNDVAWDSARQGNSDGLVILAEAQRHGRGRHGKIWLSPPGTAILMSVVLMDDSVCINP
                     GALTIAAGLAVAEGIDQASGLHTRLKWPNDVMFEGKKLAGILVERRSYKKTFVMVIGM
                     GINVFASPSNSSVDSPAASLAELVDSQKLSRNDIVQKILRRLDYWIIQVAASNLAMLH
                     DMWIPRCDMIGNRVRLKAGGILHAGRVLDVEPLGGLLLVNDLGARELIPAEGTTVVK"
     gene            complement(5930..6826)
                     /gene="nadC"
                     /locus_tag="PLK08_01770"
     CDS             complement(5930..6826)
                     /gene="nadC"
                     /locus_tag="PLK08_01770"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPS52255.1"
                     /translation="MTDRLTKRQHDCFLRLLDMALEEDLGSGDVTCLLLPKAVQTTAM
                     FIAREDMILAGECLLDDIASRYSPEIRTEHIAADGQRLASGQAIARWSGPADAIMSAE
                     RVALNFLQRLSGIATITAEYVRQVQGTRAKIYDTRKTTPGWRELEKYAVRTGGGCNHR
                     MGLHDAVLVKDNHLAALARAGRITPLAEMAPMLDELRKKLPAGGFVEVEVDTLEQLAD
                     ALRLPVDIILLDNMPPAVIRKAIEMRRSAGVEGKIEYEASGGITQKNLGEVAKTGVER
                     ISVGALTHSVQCVDIGLDSDLD"
     gene            complement(6823..7299)
                     /locus_tag="PLK08_01775"
     CDS             complement(6823..7299)
                     /locus_tag="PLK08_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52256.1"
                     /translation="MTETTDTRKRKGKLIFLVAIVAVCAAVYMYQRKDLTIPGWRSNF
                     DVALAEARKEGKMMVVLFTAQPPGEIARAIAVRVNMPANRDALFGKDGKSGRYIPACV
                     SVSKSSPLVRKYDITQLPALMVLYPDGSVRNRHDGEIGEVPFRVEFLEYKESGSKK"
     gene            7389..8792
                     /locus_tag="PLK08_01780"
     CDS             7389..8792
                     /locus_tag="PLK08_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52257.1"
                     /translation="MMENLNKSRKSAGGIAFCYLIIVLISFIFYAAFAQRTVGWQDSG
                     EYQWRVLAGDWTTSQGLARAHPLYISAGRGILLLAGKTNLVYALNLLSSLGMAIALGN
                     FFLLGNFLTGCRWIPILGAAMLGVCHTPWWLAEVAEVYTWSLAIFFGELVTMAWLIRR
                     PSSWKLCLLALLAGLELAVHGFAVLAYPVYFVVAIVLVWKKKLSLWSLADGLIFWSAG
                     ASLWLGQMIWLWERTDFLYALRNALVGMYGDKILTLGVHHELLKVNICLSGLNFLNFL
                     LPLAIIGWWNFRRRLGSGIAAAIGAITVIHVAFFIRYPVPDKFTFILPTLGLIALAAT
                     VGMDAMAERGIAWRRAVVVMATISVILGPLCYLALPKILSGHVGGLEKTRWPYRNELV
                     YWISPWKQNENSAQKYCTEKLTSLPKNATVGDNGSATDFFPMMVLQQAYDIRPDVKFR
                     SDLLPGGTMWKRNYAPQ"
     gene            complement(8761..9612)
                     /gene="folP"
                     /locus_tag="PLK08_01785"
     CDS             complement(8761..9612)
                     /gene="folP"
                     /locus_tag="PLK08_01785"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPS52258.1"
                     /translation="MKDVVRDILSSNGSGRFYVMGILNVTPDSFSDGGKYLSAEAAIS
                     QAARMIEDGADVIDIGAESTRPGSERVDADEQISRLKDILPIITEMKTVVSVDTTLSE
                     VARFAIEHGADIINDISAGTDDTRMFPLLAAHRTPVIFMHMPAQPKVMQHQPHYDDVA
                     GEVAIYLEGRLEAAVSAGLPPELCIIDPGIGFGKLPEHNLQLLRETNRLTALGRPVLI
                     GASRKRFIGAITGQKTPQERLGGTIAVCCETYRLGASIFRVHDVRPTIDALKITAAIE
                     GRNSSST"
     gene            complement(9613..>10577)
                     /locus_tag="PLK08_01790"
     CDS             complement(9613..>10577)
                     /locus_tag="PLK08_01790"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HPS52259.1"
                     /translation="RRELEKKLRDLLKEDRAKNKVLKISNFGLIQMTRQRLRPSLKQS
                     IFKMCPYCDGTGMVLSNESVALSVIRNLKVACASQDVASIELAVAPEVAHYLLNSQRQ
                     IITDIEHEFRKTITVTSRHDLSGNDVHISCINNRGAEVSWDIFRPKTPKQLASLLVEV
                     KNDLTLDTEQDNEPESNPLPVADRQTDQPQNKQQQPREKNMPAQPQPSQEKSKRKRPR
                     RKKKHQSMSQPQLPQNEIPGEIKPAVVQQEILSQQPQQSPVFEQLHEHVVAEDAQQEE
                     HAKKSRRRGKRGGRKRHKAKPQTNENAVNELSEHVSEQLQNEDE"
BASE COUNT         2408 a         2787 c         2759 g         2623 t
ORIGIN      
        1 ccgcaaaatt gctcgatttc ggcgacgccc atgtttttgg gataggttct agaaaaccgt
       61 cgtctaaggc tgctataatc atctggcacg ataattgcag agccaaaacg ggaggtacac
      121 tatatatgcg tatggacaaa cttacagtca aggcacagga agcgttcatg ggagcgcagg
      181 gccgtgcatc cgaagctggc aatcctcaaa ttaccccgct gcatctgctg gcggaaatgg
      241 ctgagcagga aggcggcgta gtagggccac tattggaaaa agtcggcatc tcgcacaacc
      301 gcctgatgac cgtaataaat gcggaactag accgcctgcc aaaacagtcc agcggcggcg
      361 gcatgggtat ggattcgacg ctgaacaacg tcatgacgca agccggcaaa gaagcaaaag
      421 cacttaagga cgattacata tcagtggagc attttcttct tgcgatgact caggaagaga
      481 gtaatgcccg cgacgtacta ctgacgctcg gtgccacgca tgacgaagtt cttaaagcca
      541 tgcaggaaat ccgcggcagt cagcgcgtta cggaccagaa tccggaagaa aaatatcagg
      601 cgctgaagaa atacggccgt gatttgtgcg agattgcgcg cagcggaaaa ctcgatcccg
      661 tcatcggccg cgacgaggaa attcgccggt gcatgcaggt actttcacgg cgcaccaaaa
      721 ataatcctgt actcatcggg ttgcctggtg tgggcaaaac ggctatcgtc gaaggcctcg
      781 ctcagcggat agtcaacggc gatgtaccga acgttctgct caacaagcgc gtaatagcgc
      841 tggacatggg tgcattgctg gctggggcga aattccgcgg cgaattcgaa gaccggctaa
      901 aagccgtcct gaatgaagtg actcaggctc agggcgaaat aattcttttc atcgacgagt
      961 tgcatacggt cgtcggcgcc ggcgcagccg aaggcgcaat atccgccggc aatctcatca
     1021 agccggctct cgcccgcggc gaactgcgct gcataggcgc cacgacgctt gatgaatatc
     1081 gcaaacatgt tgaaaaggac gcggctctcg aacgacgttt tcaacccgtg ctggtggatg
     1141 agccgtccgt cgaagatacc atcgcaatcc tgcgcggcct caagccgcgc tacgacgcgc
     1201 atcacggcgt gcgtattcag gacgccgcgc tcgtaaccgc cgccgtcatg agccatcgct
     1261 atattgctga ccgtcaactt ccggacaagg ctattgacct gattgatgag gcggcctcgc
     1321 ggctgcgtat agaaaacgac tcaatgccgt ccgaactgga cgagttgaac cgcaggataa
     1381 tgcagcttca gatagaacgt gaagcgcttc gaaaagaaag cgacgacgcc agcaaaaagc
     1441 ggctggtgga actggaaaag cagctggctg acatgcagtc gacttttgac ggcatgaaag
     1501 ccgagtggga aaacgaaaca tcgctgcttt ccaataccaa gaaactgcgc gaagcgatag
     1561 aggacaaaaa ggctcaacta gaccaggcgc aacgcaccgg cgatcttgag acggctgccc
     1621 gcattcaata tggcgaactg cgcgacctgc aaaccaaact ttcaaacgcg gaaaaggcga
     1681 ttgtggaaaa aaaggaacac ggctcgctta ccagcgaaga agtcacccct gaccagattg
     1741 ccgaagttgt cgcgaaatgg acgcatattc ccgtcagcaa actgatggaa tcggaacgcg
     1801 acaaacttct gacgatggaa gaccagctgc gcctgcgaat cgtgggtcag gacgatgcct
     1861 tgcgcgcggt agccgatgca attcgtcgca atcgcgccgg ccttggcgat ccgcacaggc
     1921 caatcggcag tttcctgttc ctcggcccta cgggcgtcgg caaaaccgaa ctctgcaagg
     1981 ctttggcgga aatgcttttc gataccgaag acgcgatggt gcgcatagac atgagcgaat
     2041 tcatggagca acactcagtg gcgcggctga ttggtgcgcc gcccggatac gtgggctatg
     2101 aagaaggcgg cagactgacg gaagccgtcc ggcgcaaacc gtactgcgtc atattgttcg
     2161 acgaagtgga aaaagcccat cccgacgttt ttaatgtgct gttgcaggtt ctggacgacg
     2221 gcaggctgac ggacggccaa ggccggactg tagacttcaa aaacaccatc atcgtcatga
     2281 cttcgaacat cgccagccaa tatattcaga gcctggccga aaaacatgcc gaagattggg
     2341 aaatagaggc tcagataaag cagacgttgc gcgactactt caggcccgaa tttttgaacc
     2401 gtatagacga gcgggtgata ttccacgctc tcggcaagga agaactcgag ggcatcgtgg
     2461 agatacaggt tcgccagctg gccaagcgtc tggcggataa aaacgtctct ctgagaattt
     2521 cgccggcggc gaaggatttg ctgtgcgaat tcggttacga cccggcgttc ggtgcaaggc
     2581 cgcttaaacg ggttatacag caatatcttg aaaacgaact cgcaaaaaaa attcttacag
     2641 gtgaagtcat ggccggccag accgtcgtgg tggatgcgca aggcaaagcc ttccacttct
     2701 cggtcgaata acaaacatga tcatggtata agtttttaaa aaaccaattt taagttaaca
     2761 acaccaggtt taattccacc agctttagtt ggcaaaaatt tataaaaaat gctttatagt
     2821 aattctcgaa agtctctgtg aatagtctgc cgtttctcct ggtattgtta atgccataat
     2881 atgcagtaac gacagcttaa aacgatggtt ttgtctttgc agatgttgcc taaacattca
     2941 cggttatttt caagaaccgc tatagaaaac actttcaaga accggaatta tggctgtcgg
     3001 ccatgctgat tgccagcacg gtaatgtcgg caggcgttat gccgctcacc cgcaacgcct
     3061 ggccgagatt cgccggctga atttttgaga gtttttcttt cgcttcccat cgtagctgtt
     3121 ttaccgaaaa ataatcaaaa ccggcgggca gtttttttgc atccaggtct ttgaatctgg
     3181 cgttatactg ctcctgtttg acggtatatc cttcgtacgc cgcgtcgatg gcgatggtat
     3241 tgacggcttg tgcgtcgaga ttttgcaatt cttgtaacgg cttgccggat tcgcctgctt
     3301 cattaatcag caggtcgatt ttcgccccgg gacgacgcat gagttcccac aaagttttgc
     3361 cttcgattct ggtcgtgtgc atgatatttt ccgccgcact gcaatttctg gatttttctt
     3421 caaaaacttt gtgacgttca gtatcgaccg taccgatatc gcagccgaca ggcgtaagac
     3481 gccggtctgc attgtcatat cgcagtgtca gccggtgttc agcgcggctg gtgaacatgc
     3541 gatacggctc ggtcacgcct ttggttatca ggtcgtctat cattacgccc atatatgcct
     3601 gctgccgcgt aagtatgagc ggtgcgcgat tctgaagttt cagtgcggca ttgatagcgg
     3661 ccatcatgcc cagcgcgccg gcctcttcat aacctgtagt gccgttaagc tggcccgcca
     3721 gatacagccc ctgcatgcat tttgtctcaa gcgtggaaaa cagctgcgtc ggctgggcga
     3781 aatcatattc aatcgcatat ccgtattgca gaattttcgc attttcaagg ccagggatgt
     3841 tggcgaccat gaaatcctgt acgtcgcgcg gcatggatgt ggttatgccg ttacagtaaa
     3901 tccagtttgt attcctgcct tccggttcga gaaaaacctg atgcgacgtt ttgtcggcaa
     3961 atctctgaac tttcgtttca aaacttgggc agtatcgcgg gccgacggat gttatctggc
     4021 cggtatacat gggcgcgcga tgaaaattat cgcgaatagc ctggtggatt ttttcgttgg
     4081 tcgccgtcag ccaacacggt atctgttcga cgtcaagttg acgattcatg aaagagaacg
     4141 gtgacggcac ggcatccccg tcctgccgcg tacatctgga gtagtcgact gtttccgccg
     4201 ccagccgagg gcatgtaccg gtcttcagcc gtcccaatgt aaggccgcaa tttcggagtg
     4261 actcgctgag ttcattcgcc gccggttcgt ttattcgtcc gccagcccat ttttccgggc
     4321 cgcgatgcat gatgcctgca agaaacgtgc ccgccgtaac tatgacggct gaacatttta
     4381 atgtgacatc cgcatcggca ttaaccggcc gatatgtcag gccgcatact tttccgccgg
     4441 aggtacatat gcgtatcgcc atgccttcga gtatcataat ccccggagtc gtcaaaagtt
     4501 tttcctgcat ccatcctgaa taggcatggc ggtcagcctg gcagcgcggc ccctgaacgg
     4561 ctgggccttt tgaggcgttg agcatgcgga actgaatacc gttagcatcg gcggcgaggc
     4621 ccatgaggcc gccgagtgcg tctatttcac gaacaatctg acctttgccg atgccgccga
     4681 tagccggatt gcaactcatc tggccgattg tgtttttata catcgtcaca agagcagcgt
     4741 ttgcgcccat tcgggctgcg gcccacactg cttcgatgcc ggcgtggccg ccgccgacta
     4801 tcactatgtc aaattccatt ttcataacgg cgaaaaattg taacagatat gcactaaaaa
     4861 aagaatgccg ccgtaaaaag attattttac gacggtcgtg ccttcggctg gtataagttc
     4921 gcgtgctccg aggtcattca ccagcagcag gccgcccaat ggttcgacat ccaacacccg
     4981 gccggcgtgc aaaatcccgc cggccttcag ccgcacgcga ttgcctatca tgtcacaccg
     5041 tggtatccac atatcgtgaa gcatcgcaag gttggaggca gctacctgaa ttatccagta
     5101 gtcgagacgg cggagaattt tttggacaat atcgttgcga gataattttt gagaatcaac
     5161 caactccgcc agcgacgcgg ctggcgaatc gaccgatgaa ttcgacggcg atgcgaaaac
     5221 gtttatgccc atgcctatta ccattacgaa cgttttttta taactgcgac gctcaacgag
     5281 tatgccggcg agctttttgc cttcgaacat cacgtcatta ggccatttga gtcttgtatg
     5341 caagccgctg gcctggtcta tcccttcggc cacggctaag ccggctgcta tcgtcagcgc
     5401 gccaggattt atgcacacgg aatcatccat gagaactacg ctcatcagga tagccgtacc
     5461 cggcgggctc aaccagattt tgccgtgtcg tccacggcca tgccgctgag cctccgccaa
     5521 tattaccaga ccgtcgctat tgccctgccg agccgaatcc catgcaacat cattggtcga
     5581 attcacctct ttaaaacaca gaatgctgcg gccgacccgc agagtatcaa ggccgtcgag
     5641 aatttttccc gcatccagca aaatatcggg ttcatgttcc tgcaagttgt aattgcaatt
     5701 tttttgcaag tcgttccaat ctcatatttt tttagaacct atcccaaaaa catgggcgtc
     5761 gccgaaatcg agcaattttg caggcagcca aggttttggg gacaggttct actatgcccg
     5821 tcgtaattct acatttttct aaaaaatgta gatgaaaaca cccgccggca aacagccaac
     5881 ccaatagcct gacaaatacg acgctacggg ttttttaatc cataaaaaat cagtccaaat
     5941 cgctatccag tccgatgtcc acgcattgta ccgaatgcgt cagtgcgccg acggaaatcc
     6001 gttcgactcc ggttttggct acctcgccaa gatttttctg cgtaatgccg ccagacgctt
     6061 catattcgat ttttccttcc acaccggctg agcgacgcat ttctatcgcc tttcgtatga
     6121 cagccggcgg catgttgtcc agcaatatga tgtcgaccgg aagtctcagt gcgtcggcaa
     6181 gttgttcgag cgtatccact tccacctcga caaatccgcc ggcgggcagt ttttttcgca
     6241 actcatcaag catgggggcc atttcagcca gcggcgtaat tcggccggca cgggctagcg
     6301 cagcaagatg attgtccttt accagtacgg catcatggag gcccatgcga tgattacagc
     6361 cgccgcctgt ccggacggca tatttttcca actcgcgcca gcctggcgta gttttgcggg
     6421 tatcgtatat tttcgcccgc gtgccctgca cctgccggac atattcggcg gtaatggtgg
     6481 cgattccgct caatcgctgt aaaaaattca gtgcgacacg ttccgccgac atgattgcat
     6541 ccgccggccc gctccatcgt gcaatagcct ggccgctggc gagccgctga ccgtcggcgg
     6601 caatgtgttc cgtacgtatt tccggactgt acctggaggc gatatcgtca agcagacatt
     6661 cgccggcgag tatcatatct tcacgcgcaa taaacatggc tgtggtctga actgctttcg
     6721 gcagcagaag gcaggttacg tcgccgctgc cgaggtcttc ttccaacgcc atatccaaca
     6781 gccgcaaaaa acaatcgtgc tgtcgttttg taagtctgtc cgtcattttt tgctgccgct
     6841 ttctttatat tcaagaaatt ccaccctgaa cggcacttcg cctatttctc cgtcgtggcg
     6901 atttcgcacc gacccgtcag gatagagaac catcaacgcg ggcagttgcg ttatgtcata
     6961 ttttcgcaca agcggcgagc tttttgagac cgatacgcac gccggtatat atctgccgct
     7021 tttgccgtcc ttgccgaaca gggcatcgcg gttggcaggc atattcaccc tcacggcgat
     7081 ggcacgggcg atttcaccag gcggctgggc agtaaaaaga actaccatca tcttgccttc
     7141 ttttcttgct tcggcaagtg cgacgtcaaa attgctccgc cagcctggta tcgtcagatc
     7201 ctttcgctga tacatgtaaa ccgccgcaca gactgcgact atcgctacaa gaaaaatcag
     7261 cttgcctttg cgttttctgg tatcggttgt ttctgtcata aaaatttctc cggtgcatga
     7321 attttaactt tacggcacgg cttgttcaag tttgcccttt aaaagaagtt ctgatatcat
     7381 aataacgtat gatggaaaat ttgaacaagt cgcgtaaatc agccggcgga atcgcgttct
     7441 gctatttgat aatcgttctg atttcgttca ttttttatgc cgcgtttgcc cagcgaaccg
     7501 tcggatggca ggatagcggc gaatatcagt ggcgggtgct tgccggcgac tggacgactt
     7561 cgcagggcct ggcgagggcg catccgctgt atatatcagc cggcagaggc atacttctcc
     7621 tggccggcaa aacaaacctt gtttacgcac tgaacctctt aagctcgctc ggcatggcaa
     7681 tagcgctggg aaatttcttt ctgctcggca atttcctgac aggctgcaga tggataccga
     7741 ttctgggggc agccatgctg ggcgtatgcc atacaccctg gtggctggcg gaggtagcgg
     7801 aagtttatac ctggtcgctg gcgatttttt tcggcgaact ggtgacgatg gcatggctga
     7861 ttagacggcc gtcgtcatgg aaactctgcc ttcttgcatt gctggcggga ttggaactgg
     7921 ctgtccatgg atttgcagtg ctggcgtatc ctgtttactt cgtggtggcg atagtactgg
     7981 tctggaagaa aaaattatcg ctgtggtcgc ttgcggacgg attgattttc tggtctgccg
     8041 gcgcatcatt atggctgggc cagatgatat ggctatggga gcggacggat tttctctacg
     8101 cactgagaaa tgccctcgtc ggcatgtatg gcgacaagat acttaccctc ggcgttcatc
     8161 acgagttgtt gaaggtaaac atctgcctgt cgggcctgaa ttttttgaat ttccttcttc
     8221 cgctggcgat aatcggctgg tggaattttc gccggcggct cggcagcgga atagctgccg
     8281 cgattggcgc cataaccgtt attcacgtcg cttttttcat acgatacccc gtgccggata
     8341 aattcacgtt tatacttccg acgctcggat tgattgcgct ggcggctacg gtcggcatgg
     8401 acgcgatggc tgaacgcggc atagcgtggc gacgggctgt tgtagtgatg gcgaccatat
     8461 ccgtaattct tgggccgctg tgctatctgg ccctgccaaa aattctgtcc ggccacgtgg
     8521 gcggacttga aaaaacgaga tggccgtatc gcaatgaact cgtctactgg atatctccat
     8581 ggaaacagaa cgaaaattcg gctcaaaaat attgtactga aaaattgacg tcattgccga
     8641 aaaatgcaac cgtaggcgac aacggcagcg cgacggattt tttcccgatg atggtgcttc
     8701 agcaggcata cgacatacgg cctgacgtaa agttcaggag cgacctgctg ccaggcggga
     8761 ctatgtggaa gaggaattac gcccctcaat agccgccgtt attttcagcg cgtcgattgt
     8821 cggccgtacg tcatgtaccc tgaatatcga cgcccccaga cgatacgtct cacagcagac
     8881 ggcgatagtt ccgccaagtc gctcctgcgg cgttttttgg ccggttattg caccgataaa
     8941 tcttttgcgg ctcgcgccta tcagaaccgg cctgcccaac gcagtcaggc ggttagtctc
     9001 acgcagcagt tgcaggttat gctccggcag ttttccgaat cctatgccgg ggtcgattat
     9061 acaaagttca ggcggcaagc cggcagaaac ggctgcctcc agacggcctt ccagatagat
     9121 ggccacttct ccggcaacat cgtcgtaatg cggctgatgc tgcattacct tcggctgagc
     9181 cggcatgtgc ataaaaatga ccggcgtacg atgcgccgcc agtaacggaa acatccgcgt
     9241 atcgtcagtg ccggcagaaa tatcattaat gatatccgcg ccatgttcaa tggcgaatcg
     9301 ggcgacctcc gaaagagtcg tatcgacgct tacgactgtt ttcatctccg taattatggg
     9361 cagaatatct ttcagccgac taatctgctc atccgcgtca acccgttcac tgccaggcct
     9421 cgtactttcc gcgccaatgt caatgacgtc tgcgccgtct tcaatcatgc gtgccgcttg
     9481 cgatattgca gcctcggctg aaagatattt tccgccgtcg gaaaagctgt ccggcgtcac
     9541 gttcaatatg cccatgacgt aaaaacggcc gcttccgttc gacgataata tatccctgac
     9601 gacgtctttc aattattcat cctcattctg cagttgttcc gaaacatgtt cggaaagttc
     9661 gttaaccgca ttttcatttg tttgcggttt ggctttgtgt cgttttctgc cgccccgttt
     9721 gccgcgacgt ctggattttt tcgcatgctc ttcctgttgt gcgtcttctg cgacgacatg
     9781 ctcatgcaac tgttcgaata caggtgattg ttgcggttgt tgcgacaaaa tctcctgttg
     9841 tacgactgcc ggtttgattt cacccggaat ttcattttgc ggcagttgcg gctgtgacat
     9901 tgactggtgt ttcttttttc ggcgtggccg ttttcttttt gatttttcct gagacggctg
     9961 gggctgagcc ggcatatttt tttcccgggg ctgttgttgt ttattctgag gctggtcagt
    10021 ttgcctgtct gcaaccggca gagggttgga ttctggctca ttatcctgtt cggtgtcaag
    10081 cgtcaaatcg ttcttcactt ctaccaacag cgacgcaagc tgtttggggg ttttgggtct
    10141 gaatatatcc cagctgactt cagccccgcg gttgttaatg catgaaatat gtacgtcgtt
    10201 gcccgacagg tcgtgccgcg acgttaccgt aattgttttc ctgaattcat gttcgatatc
    10261 agttataatc tgccgctggg aattcagaag atagtgtgct acttccggag ccacagcaag
    10321 ttctatactt gccacatcct ggctcgcaca cgccaccttg aggttgcgta tgaccgacag
    10381 cgccaccgat tcgttcgaca gtaccatgcc ggttccgtcg caatagggac acattttaaa
    10441 aatactctgc ttgagcgatg gccgaaggcg ctgtcgcgtc atctgtatga ggccgaagtt
    10501 gcttatcttg agcaccttgt ttttggccct gtcttccttc agcaggtcgc ggagtttctt
    10561 ttctagttcg cgacggt
//
LOCUS       DAONFI010000031        10460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7221_length_10460_cov_3.781163, whole genome shotgun sequence.
ACCESSION   DAONFI010000031 DAONFI010000000
VERSION     DAONFI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7221_length_10460_cov_3.781163"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>217
                     /locus_tag="PLK08_01795"
     CDS             <1..>217
                     /locus_tag="PLK08_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS52260.1"
                     /translation="IETVCRNSDFITIHTPLNDDTHHIINARVLSMMKPSAYFINTSR
                     GGMVDHDALAEALRNHTIAGAAIDVFVK"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            399..1727
                     /locus_tag="PLK08_01800"
     CDS             399..1727
                     /locus_tag="PLK08_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPS52261.1"
                     /translation="MSEKKFANVKTAIPGPKSAALLQRWHRAEADTTGFQAPVVWDSA
                     SGVVITDVDGNSYLDWTSGVLVTNVGHCHPKLVAAVQQAVAKLINSYECPTEARIKAA
                     EALVAHLPKHLDKCFFMTTGSEATESCTRIMKRKTGCFEIIGVHGGFHGRTYAAASAG
                     GLSGPKRGYGPTMPGVIRVPYPYAYRSPVGSEPGKMIDYYMTLLEDEVRANSTGALAG
                     LIVEPYLGAAGFIFPPKGYLTEIEKWCRAKGILFTLDEVQSSYGRTGRMWAMEWENLT
                     PDLVSIGKGIGNGIPTSAVAARGDVIACLGKGEMSSTVGGNPVSSTAVSTILEIMDEE
                     KLVDNSLRMGKFFMDKLLKLQEKSRYIGEVRGRGLIIGVELVKDKKTKEPAADLAKQL
                     ICRCAENGLIIGSVGIYGNVIRVAPPLVVNEAQAMESIEIFEKSILSLES"
     gene            1731..2975
                     /locus_tag="PLK08_01805"
     CDS             1731..2975
                     /locus_tag="PLK08_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPS52262.1"
                     /translation="MKPDKTLIENAINANLSNASNFLCDLISTPSLPGQETAAMNLAV
                     KVFEKIASVETVPLSNALKDDRDYSDPVSGINYDGRNNLRIVMPGSGDGKNLLLNTHM
                     DTVPPSQGQTQPYEPKIVNGEIFGRGACDAKGQVATIYLMMAALKTLGIKLCGSLAGH
                     IVVEEEVGGNGTLAMARRGENADGCVVLEPTEMRLLSSIRGAVWFHINVKGKPGHSGQ
                     AGQTRSALKSAIRIIEILDGYHADLLEKSRGDALFDKYPNPMPLTFGKLAAGNWPATA
                     PGEAEIEGVLGLLPNKTAGQVMEEMRQIIRTEGGDGIKDDFEINFMYRHDASVCPRDS
                     QIVKCMSKATESAGRTPCIDAMTASCDAWFYNNQLKIPTVVFGGGSLSVAHSNNEHMP
                     VDELAKAAEILVNLAIEWCGVK"
     gene            3064..4095
                     /locus_tag="PLK08_01810"
     CDS             3064..4095
                     /locus_tag="PLK08_01810"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPS52263.1"
                     /translation="MKALVKTKKGPGNLELMDMPVPSAGEGEVLLKILAAGVCGTDIH
                     VRDDNFPYWPPVILGHEFSGRIVKVGPGVTLFKEGDRVVGEPHTRACGHCYLCRTGNI
                     QICPEKRSPGWGIDGAMAEYLVMPEKLLHRLPDSIPDDVGAVIEPTGNAVHDVLERAR
                     VQAGEFVVVIGPGPIGLLAAMTARAAGAREVVIIGTNSDESLRLEKARQLGFKTVNCQ
                     QQKASQIVQEMTAGIGADLVVECSGSAGGIASTVELIRKKGRICAIGLPGKQQVIFPY
                     AAASFKVCDVFFCMSTSYTCWDTAINLVAAGKIPAGEIITHRRPLEEWKEVFDDIDNQ
                     RAIKAVLIP"
     gene            4109..5185
                     /locus_tag="PLK08_01815"
     CDS             4109..5185
                     /locus_tag="PLK08_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52264.1"
                     /translation="MTNRKTVRFGLLGAGLIAPFHAKSILAADGCELVAVASRTAEHA
                     AKIAGEYNCKAYSSLDELLADKTIDVINILTPNHLHYEATIRCAKAGKHVLVEKPPAM
                     SLAQTDEMIAACRQAGVKLGIMLNCRARGPVRAIKTAIEQGRFGRLLQADAYMKWYRS
                     TDYYFSGDWRSKRSCGAGVTVQHAFHYIDLLQYLMGPAAKVQARMSNMAHPQVELEDS
                     LMSFINFKNGAQGAVVASTALWPGKDVRIEITGENGTAIMVGSKMDTWKFRDEQPEDE
                     QIRRLGADTGATAASGPADFDFADHQVVIEAMAKAIRENCQPLITAESARNTLEIALA
                     MYQSAADNKIVELPISNEESIWNF"
     gene            5205..6233
                     /locus_tag="PLK08_01820"
     CDS             5205..6233
                     /locus_tag="PLK08_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS52265.1"
                     /translation="MMTNLPETIKTVSELEEVLTSPSAKLVEFMKTLDGDIMILGAGG
                     KIGPTLAEIAKHAVDKAGVKKDIIAVDVVDLPALKAKGIKTIKCNMLNLEEVQKLPKA
                     KNIIFMVGRKFGSDGAEHLTWAINVMVPHNIAQTFTDSRIAVFSTGCVYPVMDIKTGG
                     ATELIPANPVGEYAMSSLGRERMFDYFSHTACEKVVIIRLNYALDLRYGVICDIATKV
                     FNGQPLDITTGYANGIWQGDACDQIIRSLSLASAPSTILNVTGPELFSIRQVANQFGE
                     IFGKTPIIEGQENGLGYISNAAKANGLFGYPSIPLEQVIKWVAAWIQAGGENIGKPTH
                     FETQNGKY"
     gene            6258..7046
                     /locus_tag="PLK08_01825"
     CDS             6258..7046
                     /locus_tag="PLK08_01825"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPS52266.1"
                     /translation="MKIARIESDGKISHAIVEDDSYRLISGSIFGEWRKTDTVIPHGK
                     ARLLTPTVPPQIICIGANYRKHCEECHAPIPDRPVIFFKTCNTLCNPDDAVVLPKTAP
                     NEVDFEAELAIVIGRQAKNVSKNDALNYVLGYTCANDISARDVQLKQDKLWDRGKSFD
                     TFCPLGPWIATGLDGNNLNLSLELNGQLMQNSKTDDMIFDIPFIIHYLSQCMTLYPGS
                     VILTGTPFGVGMGRNPQVFLKHGDKMTVKIEGIGNLTNNVVMEK"
     gene            7181..8728
                     /locus_tag="PLK08_01830"
     CDS             7181..8728
                     /locus_tag="PLK08_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPS52267.1"
                     /translation="METESAIENEATSPGTPCINEQCSDVQAAYGESMTDNQQTVENK
                     ADCSKNASGGKIWTVGTLSYTTKGLLILFSWLMIGDFVWAIRDRATGPVAQVMLKKFG
                     ASDFIVGLLIGSLPGAIGLFLSPAISCWSDRCRTRWGRRIPFLIVPAPIAAVSMIGLA
                     FSPDIGMWLHRLVGASPDTANTFVIATFGVFWTIFDFTAITANAVFGGLVNDVVPHSV
                     IGRFYGMVRAVSLLAGIFFFYVLLGKADEYYTAIYACTGAFYGIGFTAMCFKVKEGKY
                     PPPPSLSGEKGVHPLRAVKSYFYECFSSSYYIWYYAMTALSMMSLTAAVLFGVYAAQS
                     FGLSLQGYGTCVAIAFAGSFILAYPLGILVDRLHPFRMSLLTLGLHGITTLCEFLFIR
                     DAWTFSIIFVIQQILAGSYLTAVSGLPPMLLPKSRFSQMSSAGGIVMSICGILLSMLL
                     GKFLDIMNHQYIYTFGVACIFNVLAFIAAIFLYRGFIGFGGFKSYVPPEPATIAAAET
                     SSSKASE"
     gene            8832..9587
                     /locus_tag="PLK08_01835"
     CDS             8832..9587
                     /locus_tag="PLK08_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPS52268.1"
                     /translation="MIKTGLVSITFRQLGVQEIVSLVQKAGLDGIEWGGDVHVPHGDV
                     STAEHVRDLTTKAGLETAAYGSYYRLGATDTPKFSDVLASAVALGTDKIRVWAGKKAS
                     DIADYEYRKQIVEDAKRVADLAAEKGITIVCEWHCNTLTDELDSGKKLLNDVAKDNFK
                     TYWQPSMNRLPEVCMKEIDELIPHLAGMHVYAWESTTRLPLSNHYDLWRQYFNRAKSA
                     GDFFALMEFVEGDSQDSFLRDAAVLKSLQKETI"
     gene            9584..>10099
                     /locus_tag="PLK08_01840"
     CDS             9584..>10099
                     /locus_tag="PLK08_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52269.1"
                     /translation="MMINVVLAGIGGYGEGYLNALLNDADTHNARLAGCVDPFAAASK
                     HIDVIRQRNIPVYDSLEEFYKNHSAELAVISSPIHYHKSQTITALQAGSNVLCEKPVA
                     ALVEDALAMADAAKKAKRFVAIGYQWSFSPANIELKEDILAGHYGKPKRFKTCTCWPR
                     WESYYKRGQHVQ"
     assembly_gap    10100..10109
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10110..>10460
                     /locus_tag="PLK08_01845"
     CDS             <10110..>10460
                     /locus_tag="PLK08_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52270.1"
                     /translation="DSPANNATAHYLNNMFFVAGTAINKSAEPEKIEAELYRAKSCEN
                     YDTAAVRMMTDTGVELLFLTTHSSKETIGPVLEFEFEHGKVTFSPGDFKLVGQLSHGD
                     KKVYGNPYDDEMHK"
BASE COUNT         2830 a         2629 c         2706 g         2275 t
ORIGIN      
        1 atcgaaacgg tatgccggaa cagcgatttc atcacgatac acacgccgct taacgatgac
       61 actcatcaca tcatcaacgc acgcgttctt tcgatgatga agccgtccgc ctatttcata
      121 aatacttcac gcggcggcat ggtcgaccac gacgcgcttg ccgaggcatt gcgaaatcat
      181 accatcgccg gcgcagctat cgacgttttc gtaaaagnnn nnnnnnnttg ggctggataa
      241 tgtcataatc acgccgcatc tcagctggta cagcgtggat gcggaacgcg cgatacgtga
      301 aaaaatagtt caggctatcg acatgttcat cgacgggagc gggccgagga ttccggtcaa
      361 tcccgacgct gttaaaaatc ggaagaaggt ttaaaaaaat gtcagagaag aagtttgcga
      421 acgtaaagac cgccattccc ggcccgaaat cggcggcatt gttacaacgg tggcacaggg
      481 cagaagcgga tacgacgggc tttcaggcgc ctgtggtctg ggattccgcc agcggcgtcg
      541 ttatcacgga tgtcgacggc aacagttatc tggactggac aagcggcgta cttgtcacca
      601 acgtcggaca ctgccacccc aaactggtag ccgccgtcca gcaggcagtg gccaaactga
      661 ttaacagcta cgaatgccca acagaggcac gaatcaaagc cgccgaagca ctggttgctc
      721 acctgcccaa acatctcgac aaatgctttt tcatgactac cggcagcgaa gccaccgagt
      781 cctgcacgcg aataatgaaa cgcaagaccg gttgttttga gataataggc gttcacggcg
      841 gctttcacgg ccgcacgtat gccgccgcaa gcgcgggcgg cttgtccggc ccaaaacgcg
      901 gttacggccc gacaatgccg ggcgtcatcc gcgtgccgta cccatacgcc tatcgtagtc
      961 cggtcggcag cgaacctgga aaaatgatag actactacat gaccctgctt gaagacgaag
     1021 tcagggcgaa cagcaccggt gcgctggccg gcttgattgt cgagccgtat cttggagcag
     1081 ccggtttcat cttcccgcca aagggctatc tgaccgagat tgaaaagtgg tgccgcgcaa
     1141 aaggcatact gttcacactc gatgaagtcc agagttcata cggcagaacc ggccgcatgt
     1201 gggcgatgga atgggaaaac cttacgcctg accttgtatc tatcggcaag ggtataggca
     1261 acggcattcc gacgtcggct gttgcggcaa gaggcgacgt catcgcctgc ctgggcaaag
     1321 gtgaaatgag cagcacggtc ggcggcaatc cggtctccag caccgccgta agcaccatcc
     1381 tggaaataat ggacgaggaa aaactcgtcg acaactccct gcgaatgggc aaattcttca
     1441 tggacaaact gctcaaattg caggaaaagt cacgatatat cggcgaagtc cgcggcagag
     1501 gcctgattat cggagtggaa ctggtcaagg acaagaagac caaagagccc gctgccgacc
     1561 tggcaaaaca acttatctgc cgctgtgccg aaaatggcct gattatcggc tcggttggca
     1621 tttacggcaa tgtcatacgc gtcgcgccgc cgctcgtagt gaacgaggca caagctatgg
     1681 aaagcatcga gattttcgaa aaatccatat taagcctgga gtcctgatgc atgaagccgg
     1741 ataaaacact tatcgaaaat gccatcaacg caaatctttc aaatgcgtcg aattttctgt
     1801 gtgacctgat ttcgacaccg tcactgcccg gccaggaaac ggcggcaatg aatctggcgg
     1861 taaaggtttt tgaaaagata gcctcggtcg aaaccgtccc gctgagcaac gccctgaagg
     1921 atgacaggga ctactccgac ccggtaagcg gaataaatta cgacggcagg aataacctgc
     1981 gcatagtcat gcccggcagc ggcgacggaa aaaatctttt gctcaacacg catatggaca
     2041 ccgttccgcc aagccaggga caaactcagc cgtatgagcc gaaaatcgtc aacggcgaaa
     2101 tattcggccg cggcgcgtgc gatgccaaag gacaggttgc gacgatttat ctcatgatgg
     2161 cggcattgaa gacgctgggg ataaaattgt gcggcagcct tgcgggccat atcgtcgtcg
     2221 aagaggaagt cggcggcaac ggcacgttag cgatggcgag gcgaggcgaa aacgccgacg
     2281 gctgcgtcgt actcgaaccg acggaaatgc gtctgctgag cagcatccgc ggggctgtgt
     2341 ggtttcatat caacgtcaaa ggcaagcccg gccattccgg ccaagccggc caaacccgca
     2401 gcgcgcttaa atcggcaatt cgcataatcg aaatcctcga cggctatcac gccgacctgc
     2461 ttgaaaaatc acgaggcgac gccctgttcg acaaatatcc caatccgatg ccgctgacct
     2521 tcggcaaact cgctgcagga aactggccgg ctaccgcgcc aggcgaagcg gaaatcgaag
     2581 gcgtactggg actgctgccg aacaaaaccg ccggacaggt tatggaagaa atgcgacaga
     2641 ttatccgcac ggaaggcggc gacggcatta aagatgattt tgaaatcaat ttcatgtacc
     2701 gccacgacgc atccgtctgc ccgagagact cgcagattgt gaaatgcatg tcgaaagcta
     2761 ccgaatcagc cggcagaacg ccctgcatcg atgccatgac ggcatcctgc gacgcgtggt
     2821 tttataacaa tcaactaaaa ataccgaccg tcgttttcgg cggcggaagc ctctccgtgg
     2881 cgcactcgaa caacgaacat atgcctgtcg acgaattggc aaaagcggcg gagattctgg
     2941 tcaatcttgc cattgaatgg tgtggagtaa aatgaaactc tgcggtttta aaaccgcaac
     3001 atcatatttt tcaggcagtc gcctgcccga cagacgacag ataattacga aggacatatt
     3061 aaaatgaaag cgttggtcaa aacaaaaaaa ggccctggaa atctcgaact gatggatatg
     3121 cctgtcccgt ctgccggcga aggtgaagta ttgctcaaaa tcctggcagc gggcgtatgc
     3181 ggaacggata tacatgtgcg ggacgataat ttcccatatt ggccgccagt cattctcggc
     3241 catgaattca gtggccgaat agtcaaggtc ggccctggcg taacactttt caaggaaggc
     3301 gacagggttg taggcgagcc gcatacgcgc gcttgcggcc attgttacct ctgcaggacg
     3361 ggcaacatcc agatatgtcc tgaaaaacgc agcccgggct ggggcatcga cggagcgatg
     3421 gcggaatatc tcgtcatgcc cgaaaaactg ctacaccggc tgccggactc gataccagac
     3481 gacgtcggcg ccgttattga gcctaccggt aacgcggtgc acgacgtact tgaacgtgcc
     3541 agagtgcaag ccggcgaatt tgtcgtcgta ataggcccag gccccatagg ccttctggcg
     3601 gcgatgaccg ccagagccgc cggcgcaaga gaagtcgtga ttatcggcac aaattcagac
     3661 gaatcactgc ggctggaaaa agcccgacag ctcggcttca aaaccgtcaa ctgccagcag
     3721 cagaaagcat cccagattgt tcaggaaatg acagccggca taggagccga cctggtggtt
     3781 gaatgctccg gctcagccgg cggcatagcg tcgacagttg aactgatacg caaaaaagga
     3841 cgcatctgcg ccatcggcct gccgggcaaa cagcaggtga ttttcccgta cgccgccgct
     3901 tcgtttaaag tttgcgacgt gtttttctgc atgtccacca gctatacgtg ctgggatacc
     3961 gcaataaatc tcgtggcagc tggcaagata ccagccggcg aaataatcac gcatcgccgg
     4021 ccgcttgaag aatggaagga agttttcgac gacatagaca atcaacgtgc catcaaagca
     4081 gtactcatac cataaacagg aacgcaatat gacgaacaga aagacagtac gtttcggctt
     4141 attgggcgcc ggcctgattg cgccgtttca cgcaaaaagc atactggcgg cagacggctg
     4201 cgaactggta gccgtcgcat cccgaacggc tgaacacgca gccaaaattg caggagagta
     4261 caactgcaag gcatattcgt cgctcgacga acttctggcg gataagacga tagacgtcat
     4321 aaacattctc accccaaatc atctgcatta tgaagcgaca atcagatgtg caaaagccgg
     4381 caagcacgta ctcgtggaaa aaccgccggc aatgagcctc gcccagacgg atgaaatgat
     4441 agcggcatgc cggcaggctg gcgtaaagct cggcataatg ctgaactgcc gcgcacgcgg
     4501 gccggtgcgt gcaatcaaaa cagccatcga acagggccgc ttcggcagac tgctgcaggc
     4561 ggacgcttac atgaagtggt atcgcagcac ggattattat ttttccggcg actggcgaag
     4621 caaacgcagc tgcggcgcag gcgttacggt tcaacatgct ttccattaca tagacctgct
     4681 gcaatatctg atggggccgg cggcaaaagt ccaggcccgc atgagcaata tggctcatcc
     4741 gcaggtcgaa ctggaagatt cgctcatgtc gttcataaat ttcaaaaatg gcgcacaggg
     4801 agcggtggtg gcgtcgacgg cactctggcc gggcaaggat gtaaggattg aaataaccgg
     4861 cgaaaacggc actgccatca tggtgggctc aaaaatggat acgtggaaat tcagggacga
     4921 acagccggaa gatgagcaga ttcggcggct tggcgccgac accggagcaa cggcggcgtc
     4981 agggccggct gacttcgatt tcgctgacca tcaggtggtc atcgaagcca tggccaaggc
     5041 gatacgggaa aactgccagc cgcttatcac ggcagaaagt gcgcgaaata cgctggaaat
     5101 cgcgctggcc atgtatcaat ccgcagccga caacaaaatc gtggaattac ccataagtaa
     5161 tgaagaatca atctggaatt tttaatttat tttacagagg aaaaatgatg acaaatctac
     5221 ccgaaacaat caaaacggta tccgaactgg aagaagtgct gaccagccct tcagcaaaac
     5281 tcgtcgagtt catgaagacg ctggacggcg acataatgat actgggcgcc ggcggaaaaa
     5341 tcgggcccac gcttgccgaa atcgcgaaac acgccgtaga taaagccggc gttaaaaaag
     5401 atatcatagc ggtcgatgtg gtggacctgc cggcactgaa ggccaaaggc atcaaaacca
     5461 taaaatgcaa catgctgaat cttgaggagg tacaaaaact ccccaaagcc aagaatatca
     5521 tcttcatggt cggccgcaaa ttcggctccg acggcgcaga acacctcact tgggctatca
     5581 acgtcatggt gccgcataat atcgcccaga cattcacgga ttcgagaata gcggtatttt
     5641 cgaccggctg cgtatatccc gtgatggaca tcaagacggg cggcgcgact gaactgattc
     5701 cggctaatcc ggtgggcgaa tatgcaatgt cgtcactggg ccgcgagcgg atgttcgatt
     5761 atttcagcca taccgcctgc gagaaagtcg tcataatacg cctgaattac gctctcgacc
     5821 tgcgatacgg cgtgatatgc gacatcgcca caaaagtatt taacggccag ccgctggata
     5881 tcacgaccgg ttacgcaaac ggcatctggc agggcgacgc gtgcgaccag ataatcagaa
     5941 gtctgtcgct ggcatccgcc ccgtcaacga tactcaacgt aactgggcca gaactgttct
     6001 ccatccgcca ggttgcaaat caattcggtg aaattttcgg caaaacacct ataatagaag
     6061 gccaggaaaa cggcctcggt tatataagca atgccgcaaa agccaacggc cttttcggat
     6121 atccgtcaat accgttggaa caggttataa aatgggtcgc ggcatggata caggccggcg
     6181 gtgaaaatat cggcaaaccg actcatttcg aaacacagaa cgggaaatat tgataacccg
     6241 taaaaggaag ctgcaaaatg aaaattgcga gaatcgaatc tgacgggaaa atcagccatg
     6301 cgatagttga agacgattct taccgcctga taagcggcag tatttttggc gaatggcgaa
     6361 aaacggatac ggtcataccg cacggcaaag cccgactcct cacccccacc gtgccgcctc
     6421 agattatctg cataggtgca aactatcgca aacactgcga agaatgtcac gcgccgatac
     6481 cagaccggcc agtgattttt ttcaaaacat gcaatactct ctgcaatccg gacgatgcgg
     6541 tagttctccc aaaaacagcg ccaaacgaag ttgatttcga agcggaactc gccatcgtaa
     6601 taggacgcca agctaaaaac gtttccaaaa acgatgccct caattacgtg ctgggttata
     6661 cgtgcgcgaa tgacatcagc gcacgcgacg ttcaattaaa acaggataaa ttgtgggatc
     6721 gcggaaaaag tttcgacacg ttctgcccgc tggggccatg gattgcaaca ggcctggacg
     6781 gaaacaatct caatctgagt cttgaactca acggccagct tatgcagaat tcaaaaacgg
     6841 acgatatgat tttcgacatc ccgttcataa tccattatct ttcgcagtgc atgacgctgt
     6901 atccgggcag cgtgattcta actggtacgc cttttggcgt cggcatgggc cgtaatcctc
     6961 aggttttcct caaacacggc gataaaatga ccgtaaaaat agaaggtatc ggtaatctga
     7021 ccaacaatgt tgtaatggaa aagtaatgta atatgcagag ttattcgtca attgtattgg
     7081 aaaatactgg cgaacatctc ttgccgataa ttgccatatc ataatcacgt aatgccgagt
     7141 ttgtgtttat ttgttcttta aaagatggag atgacttata atggaaacag aatcagctat
     7201 agaaaatgaa gcgaccagcc ctggaactcc atgtataaat gaacaatgtt cagatgtaca
     7261 ggcagcatat ggtgagagca tgactgacaa ccagcagaca gttgaaaata aagccgactg
     7321 ctcgaaaaac gcatccggcg gaaaaatatg gacggtcgga acgctaagtt atacgaccaa
     7381 aggcctgctg atacttttca gctggttgat gataggcgat ttcgtgtggg caatacgcga
     7441 ccgggctaca ggccctgttg cacaggtcat gcttaaaaag ttcggggcat cggactttat
     7501 tgtcgggttg cttataggtt ctctgccagg tgcaatcggg ttgtttctga gtccggcgat
     7561 aagttgctgg agcgatagat gcagaacccg ctggggccgt cgtattccgt ttcttatcgt
     7621 gccggctccc atcgcagccg tgtcaatgat tggcctcgca ttcagccccg atatcggcat
     7681 gtggttacac cggctggtcg gtgcaagtcc tgataccgcc aacacgttcg tcatcgccac
     7741 gtttggcgtt ttctggacta tttttgattt tacagcaata acagccaatg cagtatttgg
     7801 cggcctggta aacgacgttg ttccgcattc ggtaataggt cgtttttacg ggatggttcg
     7861 tgcagtcagc ctgctagccg gcatattttt cttttacgta ctgctgggca aagctgacga
     7921 atattataca gcaatatacg cctgcaccgg agctttttat ggcataggtt tcacagcgat
     7981 gtgcttcaag gtcaaggaag gcaaatatcc gccgccgcca tcgttgtccg gcgaaaaagg
     8041 cgtacaccca ctcagggcgg taaaatcata tttctacgaa tgcttttcgt cgtcatatta
     8101 catctggtat tatgcgatga cagcactgtc aatgatgtca ttaacagcgg cggttctttt
     8161 tggcgtatat gccgcacaaa gtttcggcct ttcattgcag ggttatggaa cttgtgttgc
     8221 aatagccttt gcaggttcgt tcatcctggc gtatccgctc ggcatccttg ttgacaggct
     8281 tcatccattt cggatgagcc tgctgacttt gggccttcat ggcataacca cgttatgtga
     8341 atttctcttc attcgcgacg catggacatt ttcaattatt ttcgttattc agcagatact
     8401 tgccggctca tatcttacag ctgtctcagg tctgccgccc atgcttcttc ccaagtcgcg
     8461 gttttcacag atgagttcgg ctggcggcat agtcatgagc atctgcggaa tcttgttgag
     8521 catgcttttg ggcaaatttc tggacatcat gaatcatcag tatatttata ctttcggagt
     8581 tgcctgcata ttcaacgtat tagcctttat tgcagccata tttctttatc gcggctttat
     8641 cggtttcggc ggcttcaaga gttacgtccc gccagagccg gccactatag ccgccgcaga
     8701 aacatcttcc tcaaaagcat cggaatagct tttacatagc tcctacatag ttcctacatt
     8761 cataaaagaa cactttggaa gttttttaga acctaaagta gctttccaat cggcatgaaa
     8821 ggttgttaga tatgataaaa acaggcttgg tttccattac attccgtcaa cttggcgttc
     8881 aggagatagt ttccctggtt caaaaagcag ggctggacgg cattgaatgg ggcggcgacg
     8941 ttcacgttcc ccacggcgat gtttcgacgg cagaacatgt tcgcgacctg acaacgaaag
     9001 caggccttga aacagcggca tacggttcat attaccgcct cggagcaacc gatacgccga
     9061 aattttcaga cgtgcttgca agtgccgtag cattggggac agacaagatt cgtgtatggg
     9121 ccggcaaaaa agccagcgac atcgcagatt acgaatatcg caaacagatt gtcgaagatg
     9181 ccaaacgggt agccgacctt gctgccgaaa aaggaatcac gatagtttgc gaatggcact
     9241 gcaacacttt gaccgatgaa cttgattccg gcaaaaaact tctgaatgac gtggcaaaag
     9301 ataattttaa aacttattgg caaccttcga tgaacagact gccggaagtc tgtatgaagg
     9361 agattgacga actgattcca catctggccg ggatgcatgt ttatgcatgg gagagtacca
     9421 ctcgactgcc gctgtccaat cattacgacc tttggcggca atattttaat cgtgcgaaat
     9481 cagccggcga tttttttgcc ttgatggaat ttgtcgaagg cgattcccaa gactcttttc
     9541 tgcgtgacgc ggcggttctc aagtctttac aaaaggaaac gatatgatga ttaatgtagt
     9601 tttagccggc ataggcggtt atggagaagg ctatttaaat gctcttttga acgatgccga
     9661 tacacacaac gcacggctgg ccggctgtgt agatcccttt gcagcagcat ccaaacatat
     9721 tgacgttata cggcagcgta atattccggt ttatgacagc cttgaggaat tttacaaaaa
     9781 tcattccgcc gaacttgcgg taatatcttc gcccatacat tatcacaaat cccagactat
     9841 aacggctctg caagccggct cgaatgtatt gtgcgaaaag ccggtagctg cattggtcga
     9901 agacgcatta gccatggctg atgcggcaaa aaaggccaaa cggtttgtgg ccatcggtta
     9961 tcaatggtcg ttttcaccag ccaatattga attgaaagag gatatactgg ccggccacta
    10021 cggcaaaccg aaacgattca aaacctgcac atgttggccg cgatgggaat cctattacaa
    10081 acgcggccaa catgtgcagn nnnnnnnnng gacagtccgg ccaataatgc aacggctcac
    10141 tatctcaata acatgttttt cgtagcggga actgcaataa acaaaagtgc cgaacctgaa
    10201 aagatagaag ctgaacttta tcgcgccaaa tcatgcgaaa actatgacac cgcggcagtg
    10261 cggatgatga ccgatactgg tgtcgaactt ctttttctta cgacacattc aagcaaggaa
    10321 acaatagggc ctgtcctgga atttgaattt gaacacggca aggtcacatt cagccctggt
    10381 gatttcaaac ttgtcggtca gctttcgcat ggcgacaaaa aagtttacgg caatccgtat
    10441 gacgacgaaa tgcacaaatt
//
LOCUS       DAONFI010000032        10289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7378_length_10289_cov_5.954661, whole genome shotgun sequence.
ACCESSION   DAONFI010000032 DAONFI010000000
VERSION     DAONFI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7378_length_10289_cov_5.954661"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLK08_01850"
     CDS             <1..192
                     /locus_tag="PLK08_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52271.1"
                     /translation="RRQAAKEPIAGSQGFWDRSTYKRYKKSLTTFVGFFCAIQLLRKS
                     ITGIAVLKNNTKMETCNES"
     gene            complement(766..1788)
                     /locus_tag="PLK08_01855"
     CDS             complement(766..1788)
                     /locus_tag="PLK08_01855"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52272.1"
                     /translation="MARLGKTVLSLAVCLVGVSWALAEDAAPKSQWLYAMTDSEKTPE
                     AQTIDTMDVAAPVSEEGWKKPIPVSFSVDYTLVSDYIFRGINFSEYPGEGREKPNNQM
                     TVGTELDLGSFGYIGGSVWFEWFAGQEHLNSEGDSKNLQEVDYTVYYGYNIESIGVNP
                     EIGFIWYEFPRCDENCDSTQELTLNLAFDESVWLRAMGINMKESILNPYLFMAFDLNL
                     AAGASYYETGLSHDFALADLGCSGVPVFKDITLTPSWSMSWQHNWLNKFSVDSSAGSR
                     RNFTSMNNMQWGLDLTYNIKNAFNIPDKYCGEMYVKGFIYYSQALQNQYLNDEFWGGM
                     SVGYSW"
     gene            complement(2013..2762)
                     /gene="pflA"
                     /locus_tag="PLK08_01860"
     CDS             complement(2013..2762)
                     /gene="pflA"
                     /locus_tag="PLK08_01860"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HPS52273.1"
                     /translation="MAIIRVHSIETMSGQDGPGVRCVIFLQGCPLRCTYCHNPDTWPA
                     AGGKEYSPQQLFDIVCKYKPYFKNRGGVTFSGGEPMLQARDLVPLFQLCSENNIHTAI
                     DTSGAVLNESVEECVKLADLVLLDIKHLHSDEFHTLTGGRLENLLQFVQMLSRHAKPT
                     WIRQVIVPDWNDTTKDMDELAAFLADKPFVQKVELLGYHKLADAKYLELGMTPPMPDV
                     PAMMDMRLAELQKYLNEKLAGLRINNRSNAR"
     gene            complement(2846..5245)
                     /locus_tag="PLK08_01865"
     CDS             complement(2846..5245)
                     /locus_tag="PLK08_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPS52274.1"
                     /translation="MSIDIIIKNNKTKKIVGLMLAVSICMLLLSGCKNADDKNRKSEV
                     DEKQLAAIKTIRNATVIVEIDLQYDKGESPYAGGVDCEDLVQQERPVRMAGVLVSDTE
                     AITLDPMIEKRFVKQIRVCTLDEKVDATIDSYLKEQGGAVLKFAGKLKGVTPLVFDVN
                     AKDRLYVLSSQSDPNNPAKDWTFSGSKLAVSPILTLDSEKPYKYIGANDLFLVADADG
                     KSVTLGARRKTIMPLDEKWKVAPADFLKISAADLRKLQDKLKAEFEKKIFRVALSFRS
                     PRKDVNERMAARLAENDEEADKTERDTLGIAIGENRVLVLAKLSQKSTARLQRVRVYP
                     SGKEIVAKFDKTLKNYGAFIVKTEKPLPAVQKFRKVDTNDVLYSLMLVAMPRVQGESV
                     LTYYNHTRINSDELGWRDNIYPHIFSANESDSFVFDTDMNLIAMPMANRPEPGTEERW
                     NRRRETSLTYAAQLSQIWADKFDDEDIDKSNVPLSEEDESRLAWLGVELQPMNQDLAR
                     INKVSEQTQDGSFGAIVSYIYPDSPAEKAGLELGDILLQLKVVDQPRPVKVEVGTYTF
                     GSRPFPWNELDQVPEQYYEYIPTPWVPAENKFTRALTDIGFGRKFTLEYIHDGKVCHR
                     EFVVTQSPEHYGTAKKFKSKALGLTVKDMTFEVRRYFQVLPDQAGGVIISNIEPGSKA
                     SVAGLKPYEIITHVNDMPVSDVPQFKKLLKTTAGELKLGIKRMQKGRVVKIRMAVGQP
                     NAAAKKTTAQQAQPTTQPANMEKSQIKKPAAPKNVTNKTVEQPAKSTVDTPAEKDISN
                     K"
     gene            complement(5291..6655)
                     /locus_tag="PLK08_01870"
     CDS             complement(5291..6655)
                     /locus_tag="PLK08_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPS52275.1"
                     /translation="MLKRCFIAAVGIFFAAIASAGTLPISGGVDFRTVVKSAKDKVFP
                     AVVYIKCLMENMESGKKVTHEVAGSGVIISPKGEVLTNWHVVDKAVEIRCLLLDGRYF
                     DADKIGQDKDTDLALLQLKYPKEKPQKFIYAELGDSAGMLEGDFVMAMGAPWGLNRSV
                     TMGIIACTKRYLPDNSEYSYWFQTDAAISPGNSGGPLVDTDGKVIGINTRGIMVGGDT
                     GFTIPSDTIKLIIARLREFGEANWSWTGLQLQPLKDFNKNIYFEGTQGVIVAETDPES
                     PARRGGIKQRDRIIEINGTPVTAAWSEDLPEIRRLIGLLPKNKDAVFKVIRDGKTLDF
                     KIKPRKKGKVEGEELECPRWDITVKAINQFDNPDLYFYCKQGVFVYGVKYPGNGSEAG
                     LGQQDIILKIDGKSVKTLEDVRKINEQAIKDIDKNHRMIFTVLRNGLMQQIVVDYARD
                     YEKE"
     gene            7202..9133
                     /locus_tag="PLK08_01875"
     CDS             7202..9133
                     /locus_tag="PLK08_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPS52276.1"
                     /translation="MSLVIAEHVSMSFGAEYILRDVSFRLGESDKVGLVGPNGEGKST
                     LLKILAGQLEPVSGSIHMSRGLTTAYLPQDPPAPPGKTVDDAIEDAFAALHDIEREIH
                     EITARLGRHDENDMKRLGELQTQFEVLGGYDHVSRVQRTLSGLNFPDELRGRRLDELS
                     GGQRTRASLAALLLKNPDFLMLDEPTNHLDIEATQWLEDWLKNYAGVLVVVSHDRYFL
                     DSVTNSTWQVARGGVETYRGCYSEFLPKRQQRYEERMRIWTQQQEYIAETRDFINRHI
                     AGQRTKEAQGRRTRLERFIAEEAIEKPVSPETMSLKLRTGKTTGDIVMRAEDIAVGYS
                     PAQPLVKAEKLEIVKGRRVAIVGANGIGKTTLLRSILGELELLGGKVTLGANVVPGYL
                     SQAHTELAQDMTAMEAVMALGCTGRQAMDALGAMQLGDEAAARKISELSGGQRSRVIL
                     ARLLLMESNLLMLDEPTNHLDMPSVETLQQALADFEGSVLFVSHDRYLIEKIATDIWI
                     VADGTVKTVSGGWQAYLDYCKNSAGNPTSPQQENKSRQADKEKRKEQYRQSRQRNNLL
                     QKLQKQHSDIETDIDSLERRLAGMNDEISSASVTGDMQLVEKLSLDYQACKKRLDELW
                     VRWEDVGNEIQQHCEAAVE"
     gene            9218..10129
                     /gene="dapF"
                     /locus_tag="PLK08_01880"
     CDS             9218..10129
                     /gene="dapF"
                     /locus_tag="PLK08_01880"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPS52277.1"
                     /translation="MESVEFTKLTACGNDFICIDNLSGQFDSILSDSKLSSAFARGIC
                     DRHKGVGADGVIFACKDGYTGGLAEISSAHFESDGSFCELCGNGSACFTRWVVAKGIL
                     PDRQINILTPAGIIQGQCIDRDYVRVCISPPENHRPNLNIECESADQKKTFVCDYIVT
                     GIPHVVTIVDNVSAVNVRRWGYQLRHHPAFKPRGVNANFVQILSPGRIAMRTFEFGVE
                     DETLACGTGASACAIVTALKNDWPPEIYDGTAPVIVRTRGGEILRVYITRDESGAIVD
                     CCLESRARVLYTGVLNDVFLLSTLESR"
BASE COUNT         2503 a         2660 c         2536 g         2590 t
ORIGIN      
        1 cgccgccagg ctgcaaaaga gccgattgca ggcagccaag gtttttggga taggtctaca
       61 tataaaagat ataaaaaaag cctgacaaca tttgtcgggt ttttttgtgc cattcagctt
      121 ttgcgtaaat cgataacagg catcgcagtt cttaaaaata atacaaagat ggaaacttgc
      181 aacgagtcct gataataaaa gtcccatcta tcggaacact ttagagcatg ttaaacttga
      241 gatacgacac ttttatgcgt tggcgaaacg agctatgcaa ggaaggcgaa gcaggaatac
      301 tgcccgtatt ccgatgcagc ctgacgcagc agaacgaagt tgcagctaga gcagttcaat
      361 cttgagagtt ctttttttgc tggtttgctt tgtccgtggc tgcgtcagaa aaacttcaga
      421 atactgccaa gtatgcctca ttttccttcc ttgccacgaa caaacccact ggcgcaaaaa
      481 tatgaactct caatctttca atgctctagc agaaatgatg cagagcaagt ttaaaatgca
      541 ccaagatttt cacaagaacc gctatagatg aaagactgac tgccgttttc cagcctggca
      601 agacaacagt tagccaggtg tgtccatcat attgcatttt attgtcgcat ccgtaaaaca
      661 cgactatttt accaaaaaac cgaagctccg cccggctgaa ccgtgcggaa cttcggtcta
      721 aaggaatgaa gcgttcgggg agtaaaccct gggtaatctc tagttttacc agctgtaacc
      781 tacgctcatg ccgccccaga attcatcatt cagatactga ttctgaagag cctggctgta
      841 gtaaataaat cctttgacat acatctcgcc gcaatacttg tcgggtatat tgaacgcatt
      901 tttgatgtta tacgtcaaat caaggcccca ctgcatattg ttcatgctgg tgaagttgcg
      961 acgtgaaccg gcactcgaat cgaccgaaaa cttgttcagc cagttatgct gccatgacat
     1021 cgaccagcta ggagtaagag taatatcctt aaataccggt acaccactac agcctaagtc
     1081 cgccagtgca aaatcatggc ttaagccggt ttcataatag cttgcgccgg cggcaagatt
     1141 caaatcaaaa gccatgaaca gatagggatt caatatcgat tccttcatat ttatgcccat
     1201 ggcacgcagc catacgctct catcgaatgc caggttcaag gtcagttcct gcgtactgtc
     1261 gcagttttca tcgcaacgcg gaaattcgta ccatatgaac ccgatttcag gattgacgcc
     1321 gattgattcg atattgtaac cgtaataaac ggtgtaatcc acttcctgga gattcttcga
     1381 gtcgccttca ctgttaagat gctcctggcc ggcaaaccac tcaaaccaga ccgaaccgcc
     1441 aatatagccg aacgatccga gatcaagttc agtgcctacg gtcatctggt tattcggctt
     1501 ttccctgcct tcgccaggat attcactgaa attaatgccg cggaatatat aatcactcac
     1561 cagcgtgtaa tctacactga atgaaaccgg aatgggcttt ttccagcctt cttcgctgac
     1621 cggcgcagct acgtccatgg tgtcgatagt ttgagcttca ggcgtttttt ccgaatcagt
     1681 catcgcgtac agccactgtg atttcggcgc ggcatcctcc gccaacgccc atgatacacc
     1741 aaccaggcac actgccaggc taagaaccgt ctttcctaat cgtgccattt catattctcc
     1801 tgaatacttt attgtgcagc caacaaggct acaaacttaa actcggaatt ccctgtgcac
     1861 acgccatgcc aaagaccata aaaataaaaa tacatcgatt ttacacaatg tttatcgaca
     1921 taaaatcaaa gagaacatta atgataaaag atggtaacga aagcagtgat actacaaaaa
     1981 tgtagtcaaa tagaaaattg acgacaaaaa agttatctgg cattgctgcg attattaatg
     2041 cgcaaaccag ctaatttttc attcagatat ttttgcagtt cggccagccg catatccatc
     2101 attgccggca catccggcat gggtggagtc atgccgagct caagatattt cgcgtctgca
     2161 agtttgtgat atcccagaag ttcgactttt tggacaaacg gtttgtcggc gagaaatgcc
     2221 gccaattcat ccatatcctt agtggtgtca ttccagtcag gcactatcac ctgtcgaatc
     2281 caggtaggtt tggcatggcg gctgagcatt tgaacgaact gcagcaggtt ttcgagtctt
     2341 ccgccggtga gtgtgtgaaa ttcgtcggaa tgaagatgct tgatatccag taaaacaagg
     2401 tccgccagtt ttacacattc ttcgacgctc tcattaagca ccgcgccgct ggtatctatt
     2461 gcggtatgaa tgttattttc actgcataat tgaaacagcg gcacaaggtc gcgagcctgc
     2521 agcatcggtt cgccgccgga gaatgtgacg ccgccgcgat ttttgaaata cggcttatat
     2581 ttgcacacga tgtcaaaaag ctgttgcggc gaatattctt ttccgccagc cgccggccat
     2641 gtgtccggat tgtggcaata agtgcatcgt aacggacagc cttgcaaaaa aatcacgcac
     2701 cgcacgccag ggccgtcctg gccactcatg gtctctatcg aatgaactct gattatcgcc
     2761 accggtagtt atccttcaaa acagggagca tctcacaggc actctgtacc ctgaaggttg
     2821 tttgggcatc tatttttaca cgatttcatt tattcgaaat gtctttttcc gccggcgtgt
     2881 ctacggttga ctttgccggt tgttctacag ttttattcgt cacatttttc ggcgctgccg
     2941 gttttttaat ctgagatttt tccatattag ctggctgagt tgtcggctgg gcttgttgtg
     3001 cagtcgtttt ttttgcggca gcgttcggtt gcccgacggc cattcggatt ttgaccactc
     3061 tgcctttctg catgcgcttt atgccgagtt tgagttcgcc tgctgtagtc ttgagcagtt
     3121 ttttaaactg cggcacatcg cttaccggca tatcgttaac atgcgttatt atctcatacg
     3181 gtttaaggcc agccacggaa gccttcgagc caggttcgat attggatata atcactccgc
     3241 cagcctggtc gggcagaacc tggaaatatc ttcgcacttc aaaggtcata tcctttacgg
     3301 taaggcccag tgcctttgac ttgaacttct tggctgtgcc gtaatgctca ggcgattgcg
     3361 taacgacgaa ttccctgtgg cacactttgc cgtcgtgaat atattccagc gtaaacttgc
     3421 ggccgaaacc tatatcggtc aaggcacgcg tgaacttgtt ttccgccggc acccacggag
     3481 tggggatgta ttcgtaatac tgttccggca cctggtccag ctcgttccac gggaaaggcc
     3541 ggctgccgaa ggtatacgtt ccgacctcga cctttacggg ccgcggctgg tctacgacct
     3601 tgagttgcag taaaatatcg ccgagttcaa ggcctgcctt ctccgccggc gaatcaggat
     3661 agatatacga aactattgcg ccgaacgatc cgtcctgggt ttgttcggaa accttgttta
     3721 tacgggcaag atcctgattc atcggctgaa gttcgacacc caaccacgca aggcgggatt
     3781 cgtcttcttc gctaagcggt acgtttgatt tgtcgatgtc ctcgtcgtcg aatttgtctg
     3841 cccatatctg cgaaagctgc gccgcataag taagcgacgt ttctcgccgg cgattccatc
     3901 gctcttccgt gcctggttca ggccgattcg ccataggcat ggcgatgaga ttcatgtcag
     3961 tgtcaaatac gaacgaatcc gattcgttgg cggaaaatat gtgcggatag atattatcac
     4021 gccagccgag ttcatccgaa ttgattcgcg tatggttgta atacgtcaat accgattcgc
     4081 cctgcactcg cggcatggct acgagcatca ggctgtaaag aacgtcgttg gtgtcgacct
     4141 tgcgaaattt ttgaactgcc ggcagcggtt tttcagtttt gacaataaat gcgccgtaat
     4201 ttttaagtgt tttgtcgaat tttgcgacta tttccttccc tgacggatag actcgcaccc
     4261 gttgcagcct ggcggtcgat ttctgcgaca acttggccag caccagcacg cgattttcac
     4321 ctatggcaat gcctaacgta tcgcgttcgg tcttatcggc ttcttcgtca ttttccgcaa
     4381 gacgggcagc catgcgctcg ttgacgtctt tcctgggact gcggaaactc aaggctacgc
     4441 gaaagatttt cttctcgaat tcagccttga gcttatcctg aagtttccgc aggtcggcag
     4501 cggagatttt taggaaatcc gcaggcgcca ctttccattt ctcgtcgagc ggcattatgg
     4561 ttttgcgtcg agcgccgagc gtgaccgatt tgccgtcagc atcggcgacg agaaacaggt
     4621 cgtttgcacc gatgtattta tacggctttt cactgtcgag ggtcagaatc ggcgagacgg
     4681 caagtttcga gccgctgaaa gtccaatcct tcgccggatt gttcgggtcg ctctggctgg
     4741 ataacacgta aagccggtcc ttggcgttta cgtcgaaaac cagcggcgtt acgcccttga
     4801 gtttgccggc gaatttcaaa accgctccgc cctgctcttt caggtatgaa tcaatagtag
     4861 cgtccacttt ttcatcaagc gtgcagactc gaatctgttt gacgaaacgt ttttcaatca
     4921 tggggtcgag cgtgattgct tcagtgtcgc tgaccagcac gccagccatc ctgactggcc
     4981 tttcctgctg gacgagatcc tcgcaatcca cgccgccggc atatggcgat tcgcccttat
     5041 cgtactgaag gtctatttcc actatgacgg tggcatttcg aatggttttg attgcggcga
     5101 gctgtttttc gtcgacttcc gacttcctgt ttttatcgtc cgcatttttg cacccgctca
     5161 gaagcagcat gcatatcgac acagccagca tcaagccgac gattttttta gttttattat
     5221 tttttataat aatatcgata ctcataatca aaaattcctt tcgagtattt tcgagcattt
     5281 ctgaataatt ttattctttc tcataatctc tggcgtagtc cactacaatc tgctgcataa
     5341 ggccgttgcg cagaacggta aatatcatac ggtgattttt gtcgatatcc ttgatagcct
     5401 gctcgttaat ttttctgacg tcttcaagcg tttttacgga tttgccgtct atcttgagga
     5461 ttatgtcctg ctggcccagt cccgcttccg agccgttacc cggatatttc acgccgtata
     5521 caaacacacc ctgtttgcaa tagaaataca ggtcagggtt gtcaaactgg ttgattgcct
     5581 ttactgtgat gtcccatctg gggcattcga gttcttctcc ctcgactttg cctttcttcc
     5641 tgggtttgat tttgaaatcc agggttttgc cgtcgcggat gaccttgaag acggcatcct
     5701 tgtttttcgg cagcaacccg ataagcctgc gtatctcagg caagtcctcc gaccatgcag
     5761 ccgtcaccgg cgtgccgttt atttcgataa ttctgtcgcg ttgtttgatt ccgccgcgtc
     5821 gggccgggct ttcagggtcg gtttcggcga caatgactcc ctgtgtgcct tcgaagtaga
     5881 tatttttatt aaaatctttc agcggctgaa gctgcaagcc cgtccaactc cagttagctt
     5941 cgccgaattc cctcagccgt gcaattatca gcttgattgt atcggatgga atcgtaaatc
     6001 ctgtatcgcc gccgaccata atgccgcgcg tattgatacc gatgactttg ccgtcggtat
     6061 caaccagcgg gccgccgctg ttgccggggc tgattgcggc atcggtctga aaccagtagc
     6121 tgtactcgct gttatcgggc aggtatcgtt tggtgcaggc gattatgccc atcgtaaccg
     6181 agcgattaag gccccacggc gcgcccatcg ccatcacgaa atcaccttca agcatgccgg
     6241 ctgaatcgcc aagttcggcg tagataaatt tctgcggctt ttctttcggg tatttgagtt
     6301 gcaggagggc aaggtcggtg tctttgtcct ggcctatctt gtcggcgtcg aaatatcggc
     6361 cgtcgaggag caggcagcgg atttcaacgg ctttgtcaac aacgtgccag ttggtcagca
     6421 cttcgccttt gggggaaatt attacgccgc tgccggcaac ttcatgcgta actttcttgc
     6481 cggactccat gttttccata aggcatttta tgtaaacgac ggctggaaag accttgtcct
     6541 tggcactctt gacgacagtc ctgaaatcca cgccgccgct aatcggaagc gttcctgccg
     6601 aagcaatcgc ggcaaaaaaa attccaactg ccgcgatgaa acatcttttc aacatacgcc
     6661 ggctccttgt gaaaaaatac atttttttaa catgcgtacc ggccaaatat ataccggctt
     6721 cctttaaaaa gaactctgcg aattaaaaca tagcataata gatatcatat taaaaaacct
     6781 gtcattgcgg tattttaccc gataagcgtt ctacaaacaa gaaattagag tttgcggcag
     6841 actgcgcaat aaccgtgtca gccggcccaa ataacgatta gagcagttca atcttgagag
     6901 ttcctttttt gctggtttgc tttgtccgtg gctgcgtcag aaaaacttca gaatactgcc
     6961 aagtatgcct cattttcctt ccttgccacg aacaaaccca ctggcgcaaa aatatgaact
     7021 ctcaatcttt caatgctcta gtgaaaaatc acgcagccgt tataattgtc ccaaaaaaca
     7081 ttgacacatc cgtttcgcag cgataccgtg atggcctgcc ggcggatatg cccgcctgcc
     7141 gatgcaaaaa ataaaccgta ataaaccctg ttaaagccgt atatctccgg atttaagacc
     7201 gatgtcacta gtaatcgcag aacatgtttc catgtccttc ggcgccgagt atatcctgcg
     7261 cgacgtttcg tttcgccttg gcgagtcgga caaggtcgga ctcgtaggcc ccaacggcga
     7321 aggcaaaagc acgctgctga aaatattagc cggccagctt gagcccgttt ccggcagcat
     7381 tcacatgagc agaggcctca cgacggcgta tctgccgcag gacccgcccg cgccgcctgg
     7441 aaaaaccgtc gacgacgcca tagaagacgc atttgccgcc ctgcacgata ttgagcggga
     7501 gattcacgag ataacggcgc ggcttggccg gcatgacgaa aacgacatga aacggctcgg
     7561 cgaattgcag acgcagtttg aggttttggg cggatacgac cacgtcagcc gtgttcaacg
     7621 gacgctgtcg ggcctgaatt ttcctgacga actcagaggc cgtcggctgg acgagctctc
     7681 aggcggccaa cgaacccgcg cctcgctagc cgcacttctg cttaaaaatc ccgacttcct
     7741 catgctcgat gagccgacga atcatctgga catcgaagcg acacaatggc tggaagactg
     7801 gctgaaaaat tatgcgggcg tacttgtcgt cgtcagtcat gaccgttatt tcctggattc
     7861 ggtgacgaac tctacatggc aggtcgcccg cggcggcgtg gagacgtatc gcggctgtta
     7921 cagcgaattt ctgcccaaga gacagcagcg ttacgaggaa cgcatgcgaa tctggacgca
     7981 gcagcaggaa tacatcgccg agacgcgtga tttcatcaac cgccacatcg ccggccaacg
     8041 aacgaaggaa gcgcaaggcc gtcgtacgcg gcttgaacgg ttcatcgccg aagaggcaat
     8101 cgagaaaccc gtcagccccg aaaccatgtc gctcaaactc cgaaccggaa aaaccaccgg
     8161 cgacatagtc atgcgggctg aggatatcgc cgtgggttat tcgccggctc agccgctggt
     8221 gaaagccgaa aaactcgaaa tcgtcaaagg ccgtcgggtc gccatcgtcg gagctaacgg
     8281 catcggcaaa accactttgc tacgttcgat acttggcgag cttgaactgc tcggcggcaa
     8341 ggtgacgctg ggggcgaatg tcgtaccggg ttatctttcg caggctcata ctgaactggc
     8401 gcaggacatg acggcaatgg aggcggtaat ggcgctgggc tgtaccggca ggcaggcgat
     8461 ggatgcgctg ggggcgatgc agctcggcga cgaagcggcg gccaggaaaa tctccgaact
     8521 gtccggcggc cagcggagtc gcgtcattct tgccaggctg cttctgatgg agtcaaacct
     8581 gctgatgctc gacgagccga cgaatcacct cgatatgccg tcggtggaaa ctctccaaca
     8641 ggcactggct gacttcgaag gtagtgtgct tttcgtcagc catgaccgat accttataga
     8701 gaaaattgct accgacatct ggatagttgc ggacggcacg gtcaaaacag tttcaggcgg
     8761 ctggcaggca tacctggatt attgtaaaaa ctcagccggc aatccgactt cgccgcagca
     8821 ggaaaataaa tccagacagg ccgacaagga aaaacggaag gaacaatacc gacaatcgcg
     8881 ccaacgcaat aacctgctcc agaaacttca gaaacagcat tctgatatcg aaacggatat
     8941 cgattctctc gaacgccggc ttgccggaat gaatgacgaa atatcatcag ccagcgtcac
     9001 cggcgacatg cagttggtcg aaaaactctc gcttgactac caggcctgca aaaaacggct
     9061 cgacgagtta tgggtgcgat gggaagacgt cggcaatgaa atccagcagc attgcgaagc
     9121 ggcagtcgaa tgacgatgcg tcagatgcag ccggtatcga ttttaaaagt cgacgaagac
     9181 tggataatgc gataatgggc ggctaaaatg gttcactatg gaatcagtag agttcaccaa
     9241 gctgacggca tgcggcaatg attttatctg catagacaat ctttccggcc aattcgacag
     9301 tatattgagc gactcgaaat tgtcgtcagc cttcgcgcgc ggcatatgcg accgccacaa
     9361 gggtgtggga gcggacggcg tgatattcgc ctgtaaagac ggctataccg gcggactggc
     9421 ggagatttcg tcagcccatt tcgaaagcga cgggtcgttt tgcgaactct gcggcaacgg
     9481 gtcagcctgt ttcacgcgct gggtagtggc gaagggtata ctgccggaca ggcagataaa
     9541 cattctcacg ccagccggca taatacaggg ccaatgcatt gatcgcgatt acgtccgcgt
     9601 gtgcatatcg ccgccggaaa atcatcggcc aaacctgaat atagagtgtg aatcggctga
     9661 ccagaaaaaa acgttcgtct gcgattacat cgtaaccggc attccgcacg tggtgacaat
     9721 cgtcgacaac gtgtcagccg tcaatgtccg ccgctgggga tatcagttgc gacatcatcc
     9781 cgcgttcaag ccgcgcggcg tgaatgcgaa tttcgtgcag atattgtcac ccggcagaat
     9841 agcgatgcgg acgtttgagt tcggcgtgga agacgagacg ttagcctgcg gcaccggcgc
     9901 aagcgcatgt gcaatcgtca ctgccctaaa aaacgattgg ccgccggaaa tatatgacgg
     9961 tactgcgccg gtcatagtgc gtactcgcgg cggagagatt ttgcgggttt acatcacgcg
    10021 ggacgaatcc ggcgcaatcg tggactgctg cctggaatcc cgcgcccgcg tactttatac
    10081 aggcgtactg aacgacgtgt tcctgctttc gacgctggag tcgcgatgaa cgtagtgagc
    10141 atagaccaga actcccacag cctctgggat acgttaccca aattacacgc cctcgcctca
    10201 gcaggcataa acacgacaca ctacgtcgag gatatagacg tcgcctttac ggcaaaaggc
    10261 gcaggcgtcg atgacaccag ccttaaaat
//
LOCUS       DAONFI010000033        10282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7385_length_10282_cov_4.145302, whole genome shotgun sequence.
ACCESSION   DAONFI010000033 DAONFI010000000
VERSION     DAONFI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10282
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7385_length_10282_cov_4.145302"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1650)
                     /locus_tag="PLK08_01885"
     CDS             complement(136..1650)
                     /locus_tag="PLK08_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPS52278.1"
                     /translation="MGYNAILWEIEDKVQLETCSDVIWPEALSKNEFRSILNYSRKLG
                     LEPIPLLQTIGHAEYILKHDAYCHMRELKNRHDCYCTSNLDVRSFLKNMAEEYLDMFG
                     DIQHFHIGGDEAYIFASCPKCKAAAERLNSNSLYAEHIIDIAQPIIARGVRPGIWSDM
                     MLSHPENIEYIPKNLAIWDWNYWDGDINPEAVMVWGRGRITKEQVSEVEKKTLPEIMD
                     SEGNLRSFYTTDVLRRLGYDVFICSSTRSFGDTVFCGSHDIHSHNVIGAAQKGRRSGL
                     MGHCVTSWAVRIFNLDIQEAWLAMASECSTSPETQYEDITFQVGEKIFGINPKEFYDA
                     IEKVATKIPFAVSGHDSGVQWSGLKDSLPAPANHIKSIFEKWKNEDNGKRYEEKKQEL
                     KEALLKIPQGQAKLENFTEQVTTSRGKNFCKQWLIAAKFQMRTAKMVERAFKRFENGF
                     DKIDQQGYNEIMRIRKDFENWLLYWMTPQSAKLNSELVFNPLAEWFKTTPNLHP"
     gene            complement(1821..2798)
                     /locus_tag="PLK08_01890"
     CDS             complement(1821..2798)
                     /locus_tag="PLK08_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS52279.1"
                     /translation="MTSREIILANFANDAGIRPGMDFDRGRMNDLFYGGAEPYGYTQR
                     RWTEGSREYYDDPWGNIWVRMKDGCQKGEIYKQALDNWDNLDSMTWPDFSADSCYKAV
                     RADFAADSERFHIFCLPGWIFERSRYLRRLDEYFIDMIENPEKLHVLHNKLADIFSGI
                     IVKAAEIGADAIFFCEDMGTQKGLFFSAAMWREFFGAIYRRLFGMAHDCGMKVMMHSC
                     GYNWDILPDLIAAGVDCFQFDQPLIYDTKKLALLLKQHKKVLWSPVDIQKVMPTGNRE
                     LIESAAREMVRAYRGGLICKNYTDLAGIGVKKEWDEWAYDAICHEAGIN"
     gene            3124..3900
                     /locus_tag="PLK08_01895"
     CDS             3124..3900
                     /locus_tag="PLK08_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52280.1"
                     /translation="MFHIVLFLGYNNYFSLDDKAVECKRGTLVITSPEQPHDFGPLRP
                     GTFDYHELTFSLVNGDGIELDMPIGIYLANLFGLDNLSEIPAVSECSAEKTKVICDLF
                     QEECNILKESGGKNLSACYRRIFRLLECIVETYTPHLPCVTNKKSRLEKVKKYIDSNY
                     SAGICLNDIADIANLSKYHFCRAFQKEFGVSPKAYQIELRLQAAAQLLRTSGLNCGEI
                     ADKLGFSDQYFFSKIFKKRFGVSPKNFETENWNRTGKFGR"
     gene            complement(3979..4788)
                     /locus_tag="PLK08_01900"
     CDS             complement(3979..4788)
                     /locus_tag="PLK08_01900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPS52281.1"
                     /translation="MSFYKELGRALVKLKISKYFRIRKKNFSLRFYPTAQSLVLWVDA
                     LQKKNHYQSDETFFEMYLRPEDTVIDVGANVGLYTLLAASVVGPKGHVYSIEPHPTIF
                     SYLTENIKLNHFTNIQPFNVAIGEKNGNTCFTDKKHDDINAVSDSETGIHVKLCTLDD
                     IIQTGDTVNLLKIDVEGYEKFVLTGAPRILSQTDCIFIESYESNFARFNCSTQEIIAI
                     LKQHNFFIFSYEGQTLREISEDYVSQSCENFIAVKDINKFLERTGMQIVSH"
     gene            complement(4923..6041)
                     /locus_tag="PLK08_01905"
     CDS             complement(4923..6041)
                     /locus_tag="PLK08_01905"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPS52282.1"
                     /translation="MNLFLGLYTGAACDGIDAVIISIDGKVGNDMTTEIVASESMLMP
                     DSTRRELLAIAGGKQVPADHMAKMSQDLAGSAATMGRMLIASTGIRNDDVTACGWSGF
                     EVGRRDSCILELTDQALLAHKLSMPVVSGFIASDIAAGGKGCGISAWCDWQRFRDNRL
                     TRAVVNLGGLAEMIFIPADAQPDDVIAGHVGPGTCVIDDLTFRYQHKIYDTDGSIASS
                     GKVVPELLNTLQAKPFFHENFPRSTSLFEFSPVYLFQVIQEAHKFACTGPDLIATMTE
                     LTAWSVAKAVESMTERPHEVILCGGGALNIHLADRIRRRLSPSSVYTVEKYGIPLRAK
                     KALDTAILAAARIQGFKTHIHAVTGASADCTQGSITIP"
     gene            complement(6049..6897)
                     /locus_tag="PLK08_01910"
     CDS             complement(6049..6897)
                     /locus_tag="PLK08_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52283.1"
                     /translation="MKSAAYIVVLLAAGMTAFCGLEIRDGERIIKLDPPCVAPPENGN
                     IAGTISAGKYALSTIEACCRDTSLRYKPVFWNTSDGRFEFAGLPGDAIYDIRVTTADG
                     SIFEGADMSMPDWRLEELAAARRDFLNMPQQPLLPFTISDAEEITKLVSDTKGFADIT
                     RVMIIKGHGRFAAALVELIRTGRVAGVKSGNVIWRTDIWYFERTGNAWRRMDNQERTI
                     ERLVTEPSKLAGITAAFDPKMNVKIENDGSSKTAYFTLSDSPNPSASRLAGSELQLNT
                     KPIIME"
     gene            complement(6899..7429)
                     /locus_tag="PLK08_01915"
     CDS             complement(6899..7429)
                     /locus_tag="PLK08_01915"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPS52284.1"
                     /translation="MKRQKTDYTEIFNATMKKLPSPGLLLGVYMPTGKANIMTIGWCT
                     AGFIWGMPVFSVLVRPSRYTYQGMEYSGCFTVNVPSADMNAALAVCGSKSGRDIDKFA
                     ACNLTQERAQNVLAPSIAECPIVYECQVVHSNDILPEKLSDEIMTSAYNDGDYHRVYF
                     GKIIATYAAPNASELL"
     gene            7605..8138
                     /gene="pyrR"
                     /locus_tag="PLK08_01920"
     CDS             7605..8138
                     /gene="pyrR"
                     /locus_tag="PLK08_01920"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPS52285.1"
                     /translation="MTKSEISQAIDSLCSKVAAELPEDVPVAIIGIRTRGEILARRIL
                     ERLNNELPGREINFGILDITFYRDDLSRRTGVPLVKSTEIEFDIDDVWVLLVDDVLHT
                     GRSIRAALDELHDFGRPGIVRLAVLVDRGGREMPICADYTGVKFTDIPDNERIQVRLQ
                     EKDGQEGAYIVKKKRDS"
     gene            8092..9105
                     /locus_tag="PLK08_01925"
     CDS             8092..9105
                     /locus_tag="PLK08_01925"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPS52286.1"
                     /translation="MARKAPISSKRKETPESFCANQEVRVSEGFVWSRKDLLGLDELS
                     PEEIVHILDTAESFREVSMRSIKKVPVMRGKVVALMFFEDSTRTRMSFELAAKRLSAD
                     PLIFTAKGSSVSKGESTLDTVKNIEAMGVDVFVIRHSQAGASHMVSKKTAASVVNAGD
                     GSHEHPTQGLLDIFTMRQKLGRIAGLKVAIVGDIAHSRVARSNILGLKKLGAEVIVVG
                     PPTLVPGAIKSMGCDICHNLDDVLGEVDVINMLRIQFERFGGGLFPSLREYYHLYGLD
                     SDRLKKCKNNVLVMHPGPINRGVEITSSVADGPNSCILQQVTNGLAIRMAVLFLVTSG
                     QNA"
     gene            complement(9127..9786)
                     /gene="nadD"
                     /locus_tag="PLK08_01930"
     CDS             complement(9127..9786)
                     /gene="nadD"
                     /locus_tag="PLK08_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPS52287.1"
                     /translation="MSRMLVDMAVYGQIIFGGTFDPVHLGHTGIAGEIAQTLGVTRIM
                     LLPTAGNPLKHHAAATPAQRLDMLRLATAHEIFFEICDYELFRTPPTYTIDTVEYLTS
                     AGRLTGHTGFIIGADSLNDLHHWRRVDELLTMVDLVIAVRPPEDLSSVWEIINSLKPR
                     FGNESAERMKSNIVRTKLYDISSTEIRSRAAEGKSLAGLVAPSVEEYIKTNGIYRRQP
                     R"
     gene            9909..>10282
                     /locus_tag="PLK08_01935"
     CDS             9909..>10282
                     /locus_tag="PLK08_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPS52288.1"
                     /translation="MLAKRIIPCLDVDKGRVVKGVNFVNLIDAGDPVEVARRYEREGA
                     DELVFLDITASHESRDTIVDVVKSTAEQVFMPLTVGGGIRTLEDCRRMLNAGADKVSV
                     NSAAIRNPSFINEAAKRFGSQC"
BASE COUNT         2476 a         2465 c         2523 g         2818 t
ORIGIN      
        1 aagcaaaaga cacttcgcgg gtttgacgcc gcagcgtagc gaaggcgccg cgaagcgaat
       61 cccgccctct ccgatatatt cggcggtatt acaagaatat cagccaacgt ttcttatgct
      121 acatactgta aaattttaag gatgaagatt aggagttgtt ttgaaccatt ctgctagagg
      181 attgaagacg agttcactgt taagttttgc cgattgaggt gtcatccaat acaataacca
      241 attttcgaaa tcttttctta ttcgcatgat ttcattgtag ccttgctggt caatcttatc
      301 aaagccgttc tcgaaccgct tgaatgcccg ttcgaccatt tttgctgttc tcatctgaaa
      361 tttggctgca attaaccatt gtttacaaaa atttttacca cgagaggttg tgacttgttc
      421 agtgaagttt tccaatttgg cttggccttg tgggattttc aataacgctt ctttaagttc
      481 ctgttttttc tcttcatatc ttttgccgtt atcttcattt ttccattttt caaaaatgct
      541 ctttatatga tttgccggtg ccggcagtga atctttcaat cctgaccatt gtacgccgga
      601 atcgtggcct gaaactgcaa atggtatctt ggttgccact ttttcaatgg catcataaaa
      661 ttctttcgga tttatgccaa aaatcttttc tcccacttgg aaggttatat cttcatactg
      721 tgtttctggt gaagtactac attctgacgc catggccagc cacgcttctt gaatatcaag
      781 attgaatata cgcacggccc agctggtaac gcaatgtccc atcaagcctg aacgtctccc
      841 tttttgtgca gcccctatga cattatgcga atgaatgtca tgcgagccgc aaaaaacggt
      901 atcgccgaaa gagcgggtcg aactgcatat aaaaacgtcg taacctaacc gtcgaaggac
      961 atccgtcgta taaaaacttc tgaggttgcc ttccgaatcc ataatttcag gcagtgtttt
     1021 tttctctact tcacttacct gttcttttgt aatacggcct ctgccccata ccatgacagc
     1081 ctcaggatta atgtcgccgt cccaataatt ccagtcccag atagccagat ttttgggtat
     1141 atattcgata ttttccggat gactgagcat catgtctgac catattcccg gcctgactcc
     1201 acgggcgata ataggctgtg ctatatcaat gatgtgctcg gcataaaggc tatttgaatt
     1261 cagcctctcg gcggcggcct tgcattttgg gcaggatgcg aatatgtaag cctcatcgcc
     1321 gccgatatga aaatgttgta tatcgccaaa catatcgaga tattcttccg ccatgttttt
     1381 caaaaaactt ctgacgtcaa gattggacgt acaataacag tcgtggcgat tttttaattc
     1441 tcgcatatga cagtaggcgt cgtgcttaag gatatattcg gcgtggccga tagtctgcaa
     1501 tagcggtatt ggttcaagac cgagttttcg tgaataattt aagattgagc gaaattcatt
     1561 cttcgacaat gcttccggcc aaatgacgtc gctgcacgtt tccagttgaa ccttatcctc
     1621 aatttcccat aatatcgcat tgtatcccat atcactgagt cgctcgagcc atctgcagat
     1681 gtattcttta cgaagcgaaa catgattgaa atccatgtga aatatcatgg aaaaattatt
     1741 attttggggt ggtgtcattt tatgcctatt tccatataac cggatttgag attttatttt
     1801 aacaatagct ggtacttcaa tcaatttatt ccagcttcat ggcatattgc atcatatgcc
     1861 cactcatccc attctttttt aactccgata ccggctaagt cggtataatt tttgcatatt
     1921 aaaccgcctc tatatgcacg aaccatttcg cgagctgccg actcaattaa ttccctgttg
     1981 ccggtcggca tgactttttg aatgtccaca ggcgaccata gaaccttttt atgttgcttg
     2041 agcaggaggg cgagtttttt tgtgtcgtat attaacggct ggtcaaactg gaagcagtct
     2101 acgccggctg caattaaatc cggcaaaata tcccaattat acccgcagga atgcatcatg
     2161 actttcatgc cgcagtcatg tgccatgcca aaaaggcgac gataaatagc gccaaaaaat
     2221 tcacgccaca tcgccgcgct aaagaataag cctttctgcg tacccatatc ttcgcagaaa
     2281 aatattgcat ccgctccgat ttcagccgct ttgactatga tgccgctgaa aatatctgcc
     2341 aatttattgt gcagtacgtg caatttttca ggattctcaa tcatgtcaat aaagtattcg
     2401 tctaggcgac ggagatagcg tgaccgttca aatatccagc cgggcagaca gaatatatga
     2461 aaccgttcac tgtctgctgc aaaatcagca cgaacggctt tatagcagga atcggctgaa
     2521 aaatcaggcc atgtcattga atccagatta tcccagttat ccagggcctg tttatatatt
     2581 tcaccctttt ggcagccatc cttcatccgc acccagatat taccccaggg gtcatcgtaa
     2641 tattcacgag agccttctgt ccaacgtctc tgtgtataac cgtatggctc tgcgccgccg
     2701 taaaaaagat cgttcatacg gccacggtcg aaatccattc ctggccttat gccggcatca
     2761 tttgcaaagt tagccaaaat gatttcacgt gaggtcataa atattctcca atgtgaaact
     2821 caacggttaa aacccgtaga ttacgaaatt tccgatcata tttaaatgct cttgcgattc
     2881 tgcacatatt ttatataata tagtatattg cttttataag gcgtaacaat ggcttaagtt
     2941 gctatttttt tgtattatat tgctaatatg cactatgaac tgacatcgtt gcctgccatc
     3001 atgccgcaga tgctgaattt tgattcagcc gccggaaaag ttatttttct gttcaggcat
     3061 accacattat ttcaacaaag gcagcagcga agaatcagct ctccttcctg tcatcaccat
     3121 aaagtgtttc acatagttct gttcttgggc tacaataatt atttcagcct tgatgacaaa
     3181 gcggttgaat gcaaacgcgg aacgctcgtg attacgtctc ccgagcagcc gcatgacttt
     3241 gggcctctgc gtccaggtac gtttgactat catgaactga cattctctct cgtcaatggc
     3301 gatggcattg aacttgatat gccgattgga atttatcttg ccaatctgtt cggtttggat
     3361 aatctttctg aaattcccgc cgtttctgaa tgttctgcgg aaaaaacaaa agtcatttgc
     3421 gatttatttc aggaagaatg caacatttta aaagaatccg gagggaaaaa tctttcagcc
     3481 tgttatcgac gaatattcag gctgcttgaa tgtattgttg aaacatatac gcctcatttg
     3541 ccgtgtgtta ccaataaaaa aagcagactg gaaaaggtaa aaaaatatat agacagcaac
     3601 tattccgccg gtatatgcct gaacgatatc gcggatatcg ccaatctcag taaatatcac
     3661 ttttgccgtg cttttcagaa agaattcggc gtcagcccca aagcatatca aattgaactt
     3721 cggttgcagg ctgcggcaca gctgctgcgg actagcggat tgaattgcgg cgagatagcc
     3781 gacaaattgg gattttcaga ccagtatttt ttctccaaaa tatttaagaa gcgatttggt
     3841 gtttcgccga aaaattttga gactgaaaac tggaatcgga ctggaaaatt cgggcggtga
     3901 aatcattgtg cactgattct gccttttccg gtcaatatgt ttcacaaaaa aataatttcg
     3961 agaaccgccc taaacacatt aatgcgatac gatttgcatg cctgtgcgtt caagaaattt
     4021 gttgatgtct tttacggcga taaaattttc gcacgattgg gaaacgtagt cttcgctgat
     4081 ttcgcggagg gtttgacctt cgtaagaaaa tatgaaaaaa ttgtgctgtt ttaatatggc
     4141 gataatttcc tgggtggagc agttgaatct ggcaaaattg ctttcatatg actctataaa
     4201 aatacagtca gtctggctga gaattcgcgg agctccagtc aaaacgaatt tttcataacc
     4261 ttctacgtcg attttcagaa gatttacagt atctcctgtt tggattatgt cgtccagcgt
     4321 acataatttt acatgaatgc cggtttcgct gtcggagacg gcatttatgt cgtcatgttt
     4381 tttatcggta aaacaggtat tgccgttttt ttcaccgatg gcgacgttga aaggctggat
     4441 attggtgaaa tgatttaact ttatgttttc cgtgaggtag ctgaagattg ttggatgcgg
     4501 ctcgatggaa tagacgtggc cttttgggcc tacgacggat gcagccagaa gcgtataaag
     4561 accgacattg gcgccgacgt cgataacggt atcttcaggg cgaagataca tttcaaaaaa
     4621 ggtttcatcc gactgatagt gatttttttt ctgtaaagcg tcaacccaca gaaccagcga
     4681 ttgcgctgtg gggtagaatc gtaatgaaaa attcttttta cggattctga aatacttgga
     4741 aattttcagt ttgaccaggg cacggcccaa ttctttgtaa aaactcatta tcactattcc
     4801 ttcaaaagtt gatacgtata ctacttataa tcccaaaaat tgtcaaccgt tccggtcggg
     4861 agaatcagat gattggaaat tgcttgccat ggtggcgttg cgaggctgag tacattttct
     4921 ttctatggta tcgtaatgct gccctgcgtg caatcggcgg atgctcctgt tactgcgtgg
     4981 atgtgtgttt taaagccctg aatccttgcc gccgccaata tcgccgtgtc cagagctttc
     5041 ttggctcgca gtggtatgcc gtatttttct acggtatata cgcttgacgg gctcagccga
     5101 cggcggattc tgtcggcaag atggatgttg agtgcgccgc cgccgcataa tatcacctcg
     5161 tgcggccgtt ccgtcatcga ctcgacagcc ttcgccaccg accacgccgt cagctccgtc
     5221 atagtcgcta tcaggtcagg gccggtacat gcaaatttgt gcgcttcctg gatgacctga
     5281 aacagataga cgggcgaaaa ttcgaatagt gatgtcgacc gtggaaaatt ttcgtgaaag
     5341 aacggcttag cctgcagggt gttcaaaagc tccggcacga ccttgccgga cgaagcaatc
     5401 gagccgtcgg tatcgtaaat tttgtgctga tatctgaacg tcagatcgtc aatcacgcat
     5461 gtgccaggcc cgacatggcc ggctattacg tcatccggtt gtgcgtcggc tggtatgaaa
     5521 atcatttccg ccagtccgcc gagattcacc actgcccgcg tcagcctgtt atccctgaaa
     5581 cgctgccagt cgcaccacgc cgaaattccg cagcctttgc cgcctgctgc aatatcgctt
     5641 gcgataaagc cgctgacgac cggcattgac aatttatgag ccaataacgc ctggtcggtc
     5701 aattccagta tgcatgaatc ccgacggccg acttcaaatc cgctccagcc gcatgcggta
     5761 acgtcgtcgt tcctgatgcc tgtgctcgcg atgagcattc tgcccatggt ggcggctgag
     5821 ccggcgaggt cttgtgacat tttcgccata tggtcggcgg ggacctgttt gccgccggcg
     5881 atggccagca gttcccgtcg cgtagagtcg ggcatgagca tgctttcaga cgcgacaatt
     5941 tctgtcgtca tgtcgttgcc gacttttccg tcaatcgaaa ttattacagc gtcgattccg
     6001 tcgcacgccg cgccggtgta aagtcctaaa aaaagattca ttattcggtc attccattat
     6061 tatcggtttt gtatttagtt gcagttccga gccggctagc cggctggctg acggatttgg
     6121 cgagtcgctc aacgtgaaat acgccgtttt actgctgccg tcgttttcga ttttcacgtt
     6181 cattttcggg tcgaatgccg ctgtaattcc agcgagtttt gacggttctg tgacaaggcg
     6241 ttctatcgtt cgttcctggt tgtccattcg tcgccacgcg ttgcctgtcc gttcgaaata
     6301 ccagatatcc gttcgccaga tgacgtttcc ggattttacg cccgcgactc tgccggtgcg
     6361 tatgagttcc accagtgccg cagcgaatcg gccgtgacct tttataatca ttacgcgggt
     6421 gatatctgca aaaccctttg tgtcgctgac gagtttggtt atctcttcgg cgtcgcttat
     6481 ggtgaaaggc aataggggtt gctggggcat gtttagaaaa tcgcggcgag cggcggcgag
     6541 ttcttccagc cgccagtccg gcattgacat gtcggcgcct tcaaaaatac tgccgtcggc
     6601 ggtggtgacg cggatgtcat atatcgcgtc gcctggcagg ccggcgaatt caaatcggcc
     6661 gtccgatgtg ttccagaaga ctggtttgta tcgcagactg gtgtcgcggc agcaggcttc
     6721 tatggttgaa agcgcatatt ttccggcgga tatcgtgcct gcgatattgc cgttttcagg
     6781 cggcgcaacg cagggcgggt cgagctttat aatccgctcg ccgtcgcgta tttcaagtcc
     6841 gcagaatgcc gtcattcctg cagccagcag caccacaata tatgccgccg atttcaattc
     6901 acagaagctc cgacgcgttt ggagcggcat aggtggcgat tattttcccg aaatagacgc
     6961 ggtgataatc gccgtcgttg tatgcgctgg tcattatttc atccgagagt ttttcgggca
     7021 ggatgtcgtt ggaatggacg acctggcatt catagacgat ggggcattcg gcgatgctcg
     7081 gcgcgaggac gttttgtgcg cgttcctgcg tcaggttaca tgccgcgaat ttgtcgatat
     7141 cacggccgct tttgctgccg cagacggcta acgccgcgtt catgtcggct gacgggacgt
     7201 ttaccgtgaa acatccggaa tattccatgc cctgataggt gtaacggctt ggccgtacga
     7261 gtacgctgaa aacgggcatt ccccatatga aaccggctgt gcaccagccg atggtcatga
     7321 tgttggcttt gccggtcggc atgtataccc caagcagcag cccgggagag gggagttttt
     7381 tcatggtagc gttgaaaatt tccgtgtaat ccgttttttg ccgtttcatg gttgctctcc
     7441 ttgataaaat ccaacctgat tttaccaaca tattcacggc gttgccagct ttttaaaacc
     7501 gtcgtcatcc gttggctgga tgtttctgaa atgaacggaa taggaagagg atgcttgcgt
     7561 tttggcggcg gagaggctaa aatttcacgg taatgaaatc gctgatgaca aaaagcgaaa
     7621 tatctcaggc tatcgacagc ctttgcagca aggtagccgc cgagcttccg gaagatgttc
     7681 cggtggcgat aatcggcatt cgtacccgtg gagagattct tgcccgccgc atactcgaaa
     7741 gactcaataa tgaactgccg ggccgcgaga taaatttcgg catactcgac ataacgtttt
     7801 atcgtgacga tctctcccgc cggaccggcg tgccgctggt gaaatccact gagattgagt
     7861 tcgatattga cgacgtatgg gtgctgctgg tggacgacgt tctgcacacc ggcagaagca
     7921 tacgcgccgc tcttgacgag ttgcacgatt ttggccggcc gggcattgtg cggctggcgg
     7981 tactggtgga ccgcggcggc cgtgaaatgc cgatatgcgc cgattacacc ggcgtaaaat
     8041 ttacggatat tcccgacaat gaacgcattc aggtgaggtt acaagaaaaa gatggccagg
     8101 aaggcgccta tatcgtcaaa aagaaaagag actcctgaga gtttttgtgc gaatcaggag
     8161 gttcgggtaa gcgagggctt cgtctggagc cggaaggacc tgttgggcct tgacgagctt
     8221 tcaccggagg agatagttca cattctcgac actgccgaga gttttcgtga agtttccatg
     8281 cgttcgataa aaaaagtccc cgtgatgcgc ggcaaagtgg tggcgctgat gttcttcgag
     8341 gattccacgc ggacgcgaat gagttttgag cttgccgcca aacggctcag tgccgacccc
     8401 ctgattttca ctgcgaaggg ctcgagcgtt tcaaagggcg aaagcacgct cgataccgtc
     8461 aagaatatag aggcgatggg cgtggacgtt ttcgtcatac gtcacagcca ggccggcgcg
     8521 tcgcatatgg tgtcgaagaa aacggcggcg tcggtggtca acgccggcga cggctcgcat
     8581 gaacatccga cgcagggcct gctggatatt ttcaccatgc ggcagaagct cggcagaatc
     8641 gccggcctga aagtggcgat agtgggcgat atcgcccatt cccgcgtggc gagaagcaat
     8701 atcctgggtc tgaaaaaact cggtgcggaa gttatcgttg tcggcccgcc aacgctcgtg
     8761 ccgggtgcaa tcaaatccat gggctgcgat atctgccata atctcgacga cgtactcggc
     8821 gaagtcgacg tcatcaacat gctcagaatt cagttcgaga gattcggcgg cggcctgttt
     8881 ccgtcgttac gggagtatta ccatctttac ggcctggata gcgaccggct caaaaagtgc
     8941 aagaacaacg tgttggtcat gcaccctggg ccgattaatc gcggcgtgga aatcacctcc
     9001 agcgtagccg acggcccgaa cagctgcata cttcagcagg tcaccaacgg cttggccatc
     9061 cgaatggctg tgctgttcct cgtcaccagc ggccagaacg cctgacgatg cggatgacga
     9121 atttcatcag cggggttgtc ggcgataaat gccgttggtt ttgatatatt cttccaccga
     9181 tggggcaacc aggccggcta gcgattttcc ctctgcggcg cggctgcgga tttctgtgga
     9241 cgatatgtcg tataatttcg tcctgacgat attggatttc atacgctcag ccgattcatt
     9301 gccgaaacgg ggttttaacg agttgattat ttcccatact gacgacaaat cttccggcgg
     9361 ccggactgca attaccaggt cgaccatagt tagtagttca tccacccgcc gccagtgatg
     9421 caaatcgttc agtgagtcgg ctccgattat gaatcctgtg tggccggtca atctgccggc
     9481 agaggtgaga tattcaacgg tgtctatggt gtatgtcggc ggagtgcgga aaagttcgta
     9541 atcgcaaatt tcaaaaaaaa tttcgtgtgc ggtggctaat cggagcatgt caagtcgctg
     9601 agcaggcgtg gcggcggcgt gatgttttag cggattgccg gctgtcggca gaagcattat
     9661 ccgcgtgacg cccaatgttt gggcgatttc gccggctatg cccgtgtggc ccaggtgaac
     9721 cgggtcaaac gttccgccga aaattatctg tccgtatact gccatatcta ccagcatacg
     9781 cgacatcgcg atacttttcc agtccgtaaa aacactattc acaaaaccgc cccaaatcgt
     9841 tacaatgcaa ggcctgaccg ccggttcagg cgagtcgtaa ccggctttag cgcttaaaag
     9901 gtttgaaaat gctggcaaaa cgaataatac cgtgtctgga tgttgataag ggacgtgtgg
     9961 taaaaggcgt gaatttcgtt aaccttatcg acgcaggcga ccctgttgag gtagcccgcc
    10021 gctatgagcg tgaaggtgcg gacgaactgg tctttctgga cataaccgcc agccacgaaa
    10081 gtcgcgatac catcgtggat gtggtcaaat cgacggctga acaggtgttc atgcccctga
    10141 ccgttggcgg cggcatccgc acgctcgaag actgccgcag aatgctcaac gccggcgccg
    10201 acaaggtgtc cgtgaactca gccgccatcc gcaacccgtc tttcataaat gaagccgcca
    10261 aacgcttcgg ctcacaatgc at
//
LOCUS       DAONFI010000034        10246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7419_length_10246_cov_3.935826, whole genome shotgun sequence.
ACCESSION   DAONFI010000034 DAONFI010000000
VERSION     DAONFI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7419_length_10246_cov_3.935826"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLK08_01940"
     CDS             <1..854
                     /locus_tag="PLK08_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPS52289.1"
                     /translation="FGSSRRRSDMGMDLETQVDITLEQVASGVEKTIEFERMDYCETC
                     GGTGAKPGTSPVKCKTCGGYGQVQQQAAGFFGMSVRVITCPDCHGRGTIVTNPCKNCS
                     GTGRVRKQRTLTVNIPAGIHDGQVLRVRGEGEPSLSGTAKGDLHVYVHVNEHPFFIRR
                     NDDIICSVPVSFVQAALGGTITIPTLAGPEDIEISAGVQHGHIITLKRRGLPNINGRT
                     AGHEHVQIMVEIPKKLTARQRELLVEYAATEDADVTTTRKNFINRLKDHFKDWAESLS
                     NNMQVQK"
     gene            851..1435
                     /gene="grpE"
                     /locus_tag="PLK08_01945"
     CDS             851..1435
                     /gene="grpE"
                     /locus_tag="PLK08_01945"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPS52290.1"
                     /translation="MTKHHHHHGSGNHPPQEDEQQLQAPDFTAAAEDAAQPERKTVEQ
                     ERDDLLARLQRLGADYQNYQKRVQKDISNARDFANESLLRDLLSVLDDMERAYAAAQE
                     NHPADDPLLKGMSMVHDNLLAVLQRYGLTPIESVGLQFDPEKHAAMMQQPTNEVPPMT
                     VLQEVQKGYSLKGRTIRPASVIVAKPLDETQPAE"
     gene            complement(1536..4388)
                     /locus_tag="PLK08_01950"
     CDS             complement(1536..4388)
                     /locus_tag="PLK08_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52291.1"
                     /translation="MLRKTGCSSNRGSALLMIVGLLTILALLGGTFLIVARYERKQAQ
                     ALESSPEPDARAQEIFDTIRAKLVEDLYISGTSGPYSSQEASNNPSTWRRFIDCPPST
                     DMDSSATPTLGYMRTNLYDFWLASFEPNSSDVWPQISCVVANVPDPLVYIDENASSLF
                     DTDGNTVGDSQVTDIDLSLLNGNRYSIAARVIDLSGLLALNTVGETSSTTTWTTICPT
                     SVNILGLQSTSGGVTSTINTNILSRITDGNGSNQGRNGTNTSATLESYYENCGYKLAG
                     PASNYNPFSAADELFLRWLSTDNPSKTGRIYNATSDLTSDFGPSFRKYLTATSTSRSI
                     VRHPSSFTINNGNRVPLVYTNSSGINLSNPTVREQLYTQLCEAIPNNATPANRRKIAQ
                     FVVNLWAYTATSAEIRASDFSWTPSTPSVGTVYAAVPQLVISDVFAVHISPTEIAAPP
                     ALPPFWDAAYGYAYGVELLNPTNVAIDPAEYTITSNGVSAALTSVIGTGTIEAGHSVV
                     IYKFSGKAVDKSKDISTLFATPDATWHSWNNLDFNHNNNPFSLTETRGSTTFPIDSVT
                     PSDFGWSASLDYAANTAAEEPSNAIRANRTFCAGCYRDDSVTDLGTTPATIGRDRALV
                     GGSGESGYYRPTALSGDSTTDTAITNFVHPLGRSNFTDEADATGKISLGTCAKGFYFD
                     FDSDNYSPTSTPKHAINNFADIANLYLVGPTPTQSLPMALNSYATDSSRGRIDFTTDA
                     AYYASSTTSYPDVPWPTLLNELTEVLDTDPTATVSSFAKIYGRININTAPKEVLMQLP
                     FTYIDVDGNGVQDSGEEITDATREAIVNAIINYRDDSSSRTGATNLRTNSQYRGFLTP
                     GEIAIPLRTYTTSVIGTPDSTASLKAVNSLYKAVANVVSVNSDTYAAYITVILKKPLG
                     SSETLNQWRYLAIFDRSNCTSNTDTPDALLGLTRIK"
     gene            complement(4409..5527)
                     /locus_tag="PLK08_01955"
     CDS             complement(4409..5527)
                     /locus_tag="PLK08_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52292.1"
                     /translation="MAGNVKIGLNMFRRGMTMIELLVAVSVLAILIMIFGRILSQSQR
                     VVTGSQKIMKFNASGTAAATILRKDIGQTTSAGFIYIINNYITVDGNTWPRATMYMGV
                     TGNFTSWNSSSTTSSEAIITLGLCNQNGLTSNSLPKMLWRNCTLLTSNSSDPSSITSS
                     SQDTLKYSLADTENVGSTSLTKRAFLLSNAWLGSSGLPGNSNWSPAASTDGLTLPPST
                     IDDVNMIWKYLISNVNKLSIMWTKGDYTTTNPKKLIWYGMDINGALHLPNGSGPGGIT
                     TVSYASNYDNPVSDRKYNSNVSGFLSQTEDSVYPLSATTNLAKFELHSGNNYCVLWNS
                     ELPNTYWPTALKITYEVTDGKSDNPTTKTYEIICPTGI"
     gene            complement(5536..6324)
                     /locus_tag="PLK08_01960"
     CDS             complement(5536..6324)
                     /locus_tag="PLK08_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS52293.1"
                     /translation="MKQRKNNRAFTLAEVILAIGIMGVGMVFTACLFPVAIKESESSS
                     AAMEGLIIAQNGLDVAKQMLHTSGTSNVEANTPNKTVGNFVWIDGDNSEYTAIADLPN
                     NSALGLSAYYAPTTDWNATPAPTEIPNIGCVVLLRQHDTSSNEYILISVSYRKRKTGS
                     TIKAESVSFPIGSMENTVTVGSTSEAKKFVKNGILIVDSSDETLAGKMARILGRDNNK
                     ITINHPLTNGGSISNAIVIYEDDSGNVLQETPILSATSAIVTIN"
     gene            complement(6332..7069)
                     /locus_tag="PLK08_01965"
     CDS             complement(6332..7069)
                     /locus_tag="PLK08_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52294.1"
                     /translation="MKTFKVQFSGNPHGKAAFTLIELLVTISIIALVLGIAGPTAMRM
                     FTSSSDTQAYNMFSTQLSLARAQAIESANYVGLHCQIADDDKTYVKNYKLADKCITAI
                     VAYDSGVFDLKTGEQPQEVPGNIAFGQIDTTYVSSGDYNTTVGSDMDNFTTFTILFNC
                     DGEITQKADNGNISFKSSSALFDSGTTSTGIWSLPTAEQGVTCVTMFDYITFKNKSTD
                     TLKKNYLNEAGQFIAANVYTGRLFDRE"
     gene            complement(7128..7661)
                     /locus_tag="PLK08_01970"
     CDS             complement(7128..7661)
                     /locus_tag="PLK08_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS52295.1"
                     /translation="MKRRTATGFSLVELLVAIGIIALLATIGTIAATSFSRKAKVQKT
                     HAFMDMLMSAILTYQESTGTYPNPTTNTWNQASNSSGHLYYILKNDPATFNKVTQMPS
                     ESLHMSGTSANGFVDAWGNWLYYNAKGGMGGTPALWSAGADGVGTGDAVTQRSSSYDA
                     NPTTNSNAKDDVYSYHK"
     gene            complement(7880..8338)
                     /locus_tag="PLK08_01975"
     CDS             complement(7880..8338)
                     /locus_tag="PLK08_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS52296.1"
                     /translation="MAYARTRCGFTLVEMLVVLIILSVGTALMVGVAEDVRQHSRNET
                     TRNIQAILVRALKTYKSSYGDYPAGDGKDGSSSIMLGCLQNCSRTRGELMRLPRQAIR
                     YDRNGRQTIVDGFGREMHYSRFGGLAAKNPLLYSQGQDTDDPTDDITESL"
     gene            complement(8370..9182)
                     /locus_tag="PLK08_01980"
     CDS             complement(8370..9182)
                     /locus_tag="PLK08_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52297.1"
                     /translation="MKRLYKAFTLMEILVAIGIIALLAGMAVVAFTKAKKAARKNLTM
                     TSIGMISAGAEMFKNDFGVYPPSSDKDPDESNYTNFTELGFSTNTSQLSDFKTTFNSG
                     AKLICLFLTGYPSPTNSVIIDSSNYNSDGTLKLNDVESNDACTGFGFRTRKRGKVYGP
                     YGGAEAVKTQYSGDADAENTPKVFIDSWNHAILYYRCNYDSGANTYSYDKADNQTVTV
                     DNSSSPTPYGTSGTDTKFNNFTNVRKDFLIISAGDVDNNFDWKEAYTNVSGE"
     gene            complement(9267..9974)
                     /locus_tag="PLK08_01985"
     CDS             complement(9267..9974)
                     /locus_tag="PLK08_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52298.1"
                     /translation="MTQAHKRGFTLIELLVVISIIVLLVGIIGPSISSIRNLSFKAKS
                     TAAIQELAAGAMQYKSEYNYYPLQPSIPDSGTYNSQASAAFGKLMLGYTTVMSGSTFK
                     FRSDSSTYERTPLVPESTDRFVLNTSTTIKTNRKTDTTVSVLLYTPLDQWKKGDEMAI
                     LYYPSTIGNDGTALSDVFAVSCNDTYTASTSLTNFSSKLQDTKFGTTKIVNSDSFLLI
                     APGIDGEYFTDDDVKNF"
     gene            complement(10054..>10246)
                     /locus_tag="PLK08_01990"
     CDS             complement(10054..>10246)
                     /locus_tag="PLK08_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPS52299.1"
                     /translation="DKMLYKVADTYDEEVEVMVSSMVSLLEPVMVIVLGLIVGGIVVA
                     LFMPMVSMLNAVQSTSSSS"
BASE COUNT         2545 a         2244 c         2534 g         2923 t
ORIGIN      
        1 gattcggcag ttctcgccgg cgcagtgata tggggatgga tctggagacc caggttgata
       61 ttacgcttga gcaggtggcg tctggcgtag agaagacgat tgaatttgaa cgaatggatt
      121 attgtgaaac ttgcggcggc actggcgcaa agcccggtac atcgcctgtc aaatgcaaaa
      181 cttgcggcgg gtatggccag gtacaacagc aggcggcggg ttttttcggc atgagcgtgc
      241 gggttatcac ttgtccggat tgtcacggac gtggcactat tgtgactaat ccttgtaaaa
      301 attgcagcgg tacgggcaga gtcagaaaac agcggacgct tacggtaaac attccagccg
      361 gcattcacga cggccaggtg ctgcgtgtgc gtggcgaggg tgagccgagc ctcagcggta
      421 cggccaaagg cgatttgcac gtttatgtac atgtgaatga acatccgttt ttcattcgcc
      481 ggaatgacga tattatctgt tccgtgccgg tttcttttgt tcaggctgcg cttggcggga
      541 cgattaccat tccgacgtta gccggccctg aggatattga aatttcggct ggcgtacagc
      601 acggccatat aattacgctc aagcgtcgcg gcctgccgaa tataaacggc agaacggcag
      661 gccatgaaca tgtgcagatt atggttgaaa ttcccaaaaa actcaccgca cgtcaacggg
      721 aacttttggt ggaatatgcg gctaccgaag atgccgatgt tacgacgacg aggaaaaatt
      781 ttatcaatcg cctgaaagat cattttaaag attgggcgga gtcactgagt aataatatgc
      841 aggtgcagaa atgacaaaac atcatcacca tcacggaagt ggcaaccatc cgccacaaga
      901 ggatgagcag cagttgcagg ctcccgattt taccgcagcc gctgaagatg ctgctcagcc
      961 ggagcggaaa accgtcgaac aggaacgcga cgacctttta gcccgcttgc agcggttggg
     1021 tgcggattac cagaattatc agaagcgcgt tcagaaagac atctcaaatg cgagggattt
     1081 tgcgaacgaa tcgcttttac gggatttgct cagcgtgttg gacgatatgg agcgtgctta
     1141 cgcggctgcg caggagaatc atcctgccga cgatccgttg ctcaaaggca tgtcgatggt
     1201 tcacgataac ctgttagctg tccttcagcg ttacgggttg acgcctatag agtcagtcgg
     1261 cttgcagttc gatccggaaa aacacgccgc aatgatgcaa cagccgacca atgaagtgcc
     1321 gccgatgact gtactgcagg aagttcagaa aggttattca ctcaaaggcc gtacgattcg
     1381 tccggcgtcg gtcatagtgg ctaaaccgct tgacgaaact cagccggcag aatgacgtca
     1441 ttctgaaaat cggcatcgtc catagaattt tgataaaaaa aagccccgca tttgcgaggc
     1501 ttttttcttt aaaaaaacac attctacaaa gtacgtcatt ttattcgcgt caggcccagc
     1561 aatgcatctg gcgtgtcagt gttggaagtg cagttgctgc ggtcaaatat tgccagatat
     1621 cgccattgat taagagtttc agagctgccg agcggttttt tgagaattac agtaatatag
     1681 gctgcgtaag tatcactatt gacggatact acgttggcta cggctttata gagtgaattg
     1741 acagccttaa gcgatgcagt tgaatctggc gttccaatta cacttgtagt atacgttcgc
     1801 aatggaatgg caatttcacc tggcgtaaga aagccgcgat attgagaatt cgtacgcaga
     1861 ttcgttgcac cagtacgact tgatgaatca tctcgataat tgatgattgc attgacgatt
     1921 gcttcacgtg tcgcatccgt gatttcttca ccgctatcct gtacgccgtt gccgtcgaca
     1981 tcaatatagg tgaagggtaa ttgcatcagc acctcttttg gggcggtgtt gatatttatt
     2041 ctgccgtaaa tttttgcaaa tgacgaaacc gtggcggtgg ggtcggtatc taaaacttcc
     2101 gtcagctcat tcagtaaagt gggccacggt acatccggat atgacgtagt gctgcttgcg
     2161 taataggcag cgtcagtagt gaaatcaata cggcctctgg aagaatcagt tgcataagaa
     2221 ttcaacgcca ttggcagact ttgtgtaggg gtggggccga caagataaag gttggcaata
     2281 tcggcaaagt tgttaattgc gtgttttggc gttgaggtag gtgaataatt atcgctgtcg
     2341 aagtcgaaat aaaaaccttt tgcacacgtt ccgagagaaa ttttgcctgt ggcatcagcc
     2401 tcgtcggtga aattacttct tcccagcgga tgtacgaagt tggttatggc agtgtcagtt
     2461 gtagaatcgc cgcttaatgc cgtcggacga taatagccgg attcgccact tccgccgaca
     2521 agagctctgt cgcggccaat cgttgccgga gttgtgccga gatctgttac gctatcatcg
     2581 cggtagcatc ctgcgcagaa tgtacggttt gctctgatag cattgcttgg ttcttccgct
     2641 gcggtgtttg ccgcgtaatc cagacttgcc gaccagccga agtctgacgg agtgacggaa
     2701 tctatcggaa acgtcgtgct gcccctcgtc tcagtaagac tgaatggatt attgttatgg
     2761 ttaaaatcca aattgttcca gctgtgccat gtagcgtctg gagttgcaaa gagcgtagat
     2821 atatcttttg acttgtcgac tgctttgccg ctgaatttgt atattactac gctgtgcccg
     2881 gcctcaatag tgccagttcc tatgacagat gtcaatgccg cactgacgcc gttactggtt
     2941 attgtgtatt cggcggggtc aatagctaca ttggtcggat taagcagctc tacaccatat
     3001 gcataaccgt aagcagcgtc ccagaatgga ggtaaagccg gcggagcggc aatttcggtg
     3061 ggggagatgt gaactgcaaa aacgtcgctt atgacaagtt gagggacggc tgcataaaca
     3121 gtgccgacac ttggcgttga tggtgtccat gaaaaatcgc tggcccgtat ttctgcggaa
     3181 gttgccgtat aagcccataa atttacaaca aactgggcaa tttttctacg gtttgcagga
     3241 gtagcgttat tcgggatggc ttcacataac tgggtgtaaa gttgttcccg taccgtcggg
     3301 tttgacaggt tgattccgga tgaatttgtg taaaccaacg gaacgcggtt gccgttattt
     3361 atcgtaaaac tcgatggatg tcgcacgatg cttcgtgatg tacttgtagc tgtaagatat
     3421 ttccggaatg atgggccgaa atcactggtt aaatcggagg ttgcattata tattctgccg
     3481 gttttacttg ggttgtccgt gctcagccat cgcaggaaca gttcgtcggc tgcgctgaac
     3541 ggattgtaat ttgatgcagg gccggcgagt ttatatccgc aattttcata atagctctct
     3601 aatgttgcac tggtgtttgt gccgttacgg ccttgattag aaccatttcc gtcggtgatt
     3661 cgagacagaa tatttgtgtt gattgtggac gttactccgc cggacgtact ttgcaagccg
     3721 agaatattca cgctcgttgg acaaatagtt gtccatgtgg tcgtggacga agtttcgcca
     3781 acggtattca atgccagcag gcctgaaagg tcaataacac gagcggcaat ggaatatctg
     3841 ttgccgttga gcagggaaag gtcaatgtct gtaacttgtg agtcgccgac agtgttgccg
     3901 tcggtatcga acaggctgct tgcattttca tcaatgtata ccaaagggtc aggcacattt
     3961 gcaaccacac atgatatctg cggccaaaca tcgctgctgt ttggttcaaa actggctaac
     4021 cagaaatcat aaaggttggt tcgcatgtaa cctaaagttg gtgttgccga tgaatccatg
     4081 tccgtggatg gaggacaatc tataaaacgg cgccaggtcg atggattatt actggcttcc
     4141 tgtgatgagt atggcccgct cgtgccgctg atatataggt cttcaacaag tttggcacgg
     4201 atggtatcaa atatttcctg tgccctcgca tcaggttcag ggcttgactc caacgcctgc
     4261 gcctgcttac gctcataacg ggcgactatc aggaatgtgc ccccgagcag ggctaatatt
     4321 gtcagtaacc ctacaatcat cagcaatgct gacccgcgat ttgaactgca acctgttttg
     4381 cgaagcataa tcgtttcctt tctcggcgtc agatgccggt cgggcagatt atttcatatg
     4441 tctttgttgt cggattgtca gatttgccgt cggtcacttc atacgtaatc ttgagagccg
     4501 tcggccaata agtgttgggt aattccgaat tccacaaaac gcaataatta ttgccgctat
     4561 gaagttcaaa tttagccaga ttcgtcgtag ccgaaagcgg ataaaccgaa tcttctgttt
     4621 gcgataagaa tccgcttacg ttgctattat actttctgtc tgaaacagga ttatcgtaat
     4681 tgcttgcata ggagacggtt gtaatgccgc cagggcctga accgttaggc agatgaagcg
     4741 caccattaat gtccataccg taccatatca actttttggg attagttgtt gtatagtcgc
     4801 ctttggtcca cataatcgaa agtttgttga cgttcgatat gagatatttc catatcatgt
     4861 ttacatcatc tattgttgaa ggtggtaatg tcaacccgtc ggtggaggct gccggcgacc
     4921 agttggagtt accgggaaga ccactactgc ccaaccatgc gtttgaaagc agaaaggcac
     4981 gttttgtcaa acttgtactg ccgacgtttt cagtatcggc aagcgagtat ttcaatgtat
     5041 cttgtgaaga ggaagtgata ctactggggt cgctggagtt tgatgtcaat aaagtgcaat
     5101 tccgccagag catttttggc agactgtttg aagtaagacc gttttgattg cataagccta
     5161 aggttattat ggcttccgaa cttgtagtgc tcgaactatt ccaacttgtg aaatttcctg
     5221 ttacgcccat atacatagtt gcacgcggcc acgtatttcc gtcgacagta atgtagttgt
     5281 ttatgatgta aataaaaccg gctgacgttg tttgtccgat atccttacgc aatatggttg
     5341 ctgcagcagt accgctggcg ttgaatttca ttattttctg cgagccggta accacccttt
     5401 gagactggct gagtatgcgg ccgaaaatca ttatgagaat ggcaagcaca gacactgcaa
     5461 caagcagttc aatcatggtc atgccgcggc ggaacatatt taatccgatt tttacgttac
     5521 ctgccatatt atgcctcagt taatggttac tattgccgaa gttgcagaga gtatcggcgt
     5581 ttcctgcaaa acatttccgg agtcatcttc gtaaatgacg atagcattac ttatacttcc
     5641 gccgttggtt agcgggtgat tgatggtaat tttattattg tcacggccca ggatgcgtgc
     5701 cattttgccg gcaagtgttt cgtccgatga atctacaatc agaatgccgt tcttaacgaa
     5761 tttttttgcc tcacttgtcg aacccactgt tacagtattc tccatactgc caatcggaaa
     5821 agatacgctt tcggctttta tggtgctgcc ggttttgcgt ttgcggtatg aaactgaaat
     5881 caagatatat tcattcgagc tggtgtcatg ctgcctcagt aaaacaacgc agccaatatt
     5941 gggaatctcc gtaggcgccg gtgttgcgtt ccaatcggtt gtaggagcgt aatacgcgct
     6001 caaacctaat gcggaattgt tcggcaaatc cgcaatagcg gtgtattcac tgttatcgcc
     6061 gtctatccag acgaaattgc cgacagtttt atttggggta ttggcttcga catttgatgt
     6121 gccggaagtg tggagcatct gttttgccac gtcgaggccg ttttgtgcga ttataaggcc
     6181 ttccatggcg gcggatgacg actcgctttc ttttatggca acgggaaata gacatgcggt
     6241 aaagaccatg ccgactccca ttatgccgat ggctaatata acttcagcca atgtaaaggc
     6301 tcgattgttt tttctttgct tcatacgtcc attactccct gtcgaacagg cggccggtat
     6361 atacgtttgc agctatgaat tggcctgctt catttaaata attcttcttg agcgtatctg
     6421 tgcttttgtt tttaaacgtt atatagtcga acatcgttac gcaggtaacg ccctgttcgg
     6481 ctgtgggcag actccatatg ccggtactgg ttgttccgct gtcaaataag gctgacgatg
     6541 acttgaaact tatattacca ttatcagctt tttgggtgat ttcgccgtca cagttgaaaa
     6601 ggattgtaaa tgttgtgaaa ttgtccatgt cgctacctac agtcgtattg taatctcctg
     6661 atgatacgta tgtagtatcg atttggccga atgctatgtt tcctgggact tcctgcggct
     6721 gttcaccggt ttttaggtca aaaacgccgg aatcatatgc gacgattgcc gttatgcact
     6781 tatcagcaag tttgtagttt tttacataag ttttatcgtc gtcggcaatc tggcagtgta
     6841 agccgacgta gttggctgat tcaatagcct gtgcgcgagc cagcgataac tgcgtcgaaa
     6901 acatgttata cgcctgtgta tcggaactgg atgtaaacat gcgcatggct gtcggccctg
     6961 cgatgccgag tacgagggct attatcgaaa tcgtcaccag cagctcgatg agcgtaaagg
     7021 cggcttttcc atgaggattg cctgaaaatt gtactttgaa tgtcttcatc caattatctc
     7081 gcattcagca aaaatttttc aaaaaacaaa tatcgacggc tgaaaattta tttgtgatac
     7141 gaatatacgt cgtctttagc gttggaattt gtagttggat tcgcatcata tgaactggaa
     7201 cgctgtgtta cggcatcacc agtaccgacg ccgtctgcgc cggcgctcca gagtgcaggt
     7261 gtgccgccca taccgccttt ggcattgtaa tacaaccagt taccccaggc atccacgaaa
     7321 ccatttgcag aggtaccgct catgtgtaat gattccgagg gcatctgtgt tactttattg
     7381 aatgtagccg gatcattttt gagtatataa tacagatggc cgctggaatt gctggcttga
     7441 ttccacgtat ttgtcgtcgg attgggataa gtgcctgtgc tttcctggta agtgagaatt
     7501 gcactcatta gcatgtccat gaaagcgtgg gttttctgta cctttgcttt gcgggaaaaa
     7561 ctggtggctg cgattgtgcc tatggtagcc agcagggcga taatgcctat cgccaccagt
     7621 aactcgacga gcgaaaaacc ggttgcagtt ctacgtttca ttataaaaat tccttaacta
     7681 aaataacaag aatcaaaaaa caggactcaa gcaaatttca attttcaatt ttacgagtaa
     7741 cggcggctgc aatttttaat ttattccttc aacgttgctt gcagatttaa aaaccttgtt
     7801 acctgtaatt tatttgacga acatagccgg cgatggccga gcctgccagt caatccactg
     7861 attcactttt tgaaaaatcc tacagacttt ctgttatatc gtctgtcggg tcatccgtat
     7921 cctggccttg gctgtagagc agggggttct ttgccgccag tccgccgaac cggctgtaat
     7981 gcatttcccg cccgaagccg tccactatgg tctgtcgacc attcctgtca taacgtattg
     8041 cctgccgcgg cagacgcatt aattcgcctc gcgttcgtga gcagttctgc agacaaccca
     8101 gcattatgga actgctgcca tctttgccgt cgccggcggg ataatcgcca tatgaacttt
     8161 tatatgtttt cagggctcga acaaggatgg cctgtatatt acgggttgtt tcattgcggc
     8221 tgtgttgccg gacatcttcg gcgacaccca ccatcaaagc cgtgccgaca gacagaatta
     8281 tcagcacaac cagcatctca accaacgtaa aaccacatct tgtacgtgcg tatgccatta
     8341 atcggttttt ccatgtttca cttttgcaat tattcgccgg aaacgttcgt atatgcttct
     8401 ttccagtcaa aattattgtc gacatcgcca gccgaaatta tcagaaaatc ctttcgcaca
     8461 ttagtaaagt tgttgaattt tgtatctgtt ccgcttgtgc cataaggcgt tgggcttgat
     8521 gaattatcaa ctgttactgt ttgattgtcg gctttgtcgt atgaataagt attggcgccg
     8581 gaatcataat tacatctata ataaagtatt gcatgattcc aactgtcgat aaaaactttc
     8641 ggcgtatttt cagcatcggc atcgccggag tattgagttt ttacggcttc agcgccgcca
     8701 taggggccgt aaactttgcc tcgcttgcga gtacggaaac cgaagccggt acaggcgtca
     8761 ttggattcaa cgtcatttaa tttcagcgtg ccgtcactgt tgtaattcga agaatcaatt
     8821 ataactgaat tggtcgggct tggataaccg gtcaaaaaca aacatatcag ctttgcaccg
     8881 ctgttgaatg tagttttgaa atcactcaat tgacttgtgt tagtagaaaa acccagttcg
     8941 gtgaaatttg tataatttga ttcgtccgga tctttgtccg aacttggagg atagacgccg
     9001 aagtcgtttt tgaacatttc agcgccggcg gaaatcatac cgatggaggt catggtaaga
     9061 ttctttcttg cggctttctt ggcttttgtg aaagcgacga cagccatgcc ggcgagcagg
     9121 gcaattatgc cgatggcgac aagtatttcc ataagcgtga atgctttata taagcgtttc
     9181 ataagtcatt tcctgataaa aaattcaaat tacagatttt taaatggaaa ccggtaatcg
     9241 tttcacccgt cgattttcaa aaaacgtcaa aaattcttga catcgtcatc cgtaaaatat
     9301 tcgccgtcaa ttcccggagc tatcagcagg aaagaatccg aattgacgat tttggtagtg
     9361 ccgaacttgg tgtcctggag tttggaactg aaattcgtca agctcgttga agctgtatat
     9421 gtatcattgc acgagactgc aaaaacatcg cttaaagctg tgccgtcgtt acctatagtt
     9481 gacgggtagt aaagaatggc catttcatcg ccttttttcc actgatcaag tggtgtatat
     9541 aaaaggactg aaacggttgt gtcggttttt cggtttgttt ttatggtggt tgatgtattt
     9601 aaaacaaatc tgtcggtcga ttccggaacc aatggcgtac gctcatacgt tgacgagtcg
     9661 ctccggaatt tgaaagttga accggacatg acagttgtat agcccagcat caatttgccg
     9721 aaagcggctg aagcctgaga attatatgta cctgagtcgg gtatgcttgg ctgcaacggg
     9781 tagtagttgt attcgctttt gtattgcatt gcgccggcag ccaactcctg tatggctgcg
     9841 gtggatttgg ccttaaaact cagattacgt attgaactga tacttgggcc tattatgccg
     9901 accagcaaaa ctataatgct gatgaccact aacagttcaa taagcgtaaa acctctttta
     9961 tgtgcttgcg tcatatatta tcaatcctta aatgcctgct gttgcaataa tcaatcggct
    10021 gttttagccg gaattaattt tatttccgct tttttatgac gacgacgagg tagactgtac
    10081 tgcgttgagc atgctgacca tcggcatgaa cagcgcgacc actatgccgc cgactatgag
    10141 gccaagcacg atgaccatga ccggctcaag cagtgaaacc atggacgaga ccatgacttc
    10201 aacttcttcg tcgtaggtgt cggcgacttt gtaaagcatt ttgtcg
//
LOCUS       DAONFI010000035        10236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7429_length_10236_cov_4.347412, whole genome shotgun sequence.
ACCESSION   DAONFI010000035 DAONFI010000000
VERSION     DAONFI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7429_length_10236_cov_4.347412"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLK08_01995"
     CDS             complement(<1..412)
                     /locus_tag="PLK08_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52300.1"
                     /translation="MIVRELIVVVVLIAGAIISCKNTTGYEKESTMNTPEMTVKIGWG
                     QADITPSMPVLIAGQFCARVSESVLDPITITAMAIEGEDQAVMVSCDLVCIPDELRDE
                     VRSRLKKAANGVDPMKVVFNATHTHTAPEMRVDIK"
     gene            650..1468
                     /locus_tag="PLK08_02000"
     CDS             650..1468
                     /locus_tag="PLK08_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52301.1"
                     /translation="MKFAHAYLPEIRTLGLMQFDPIWAQRMHETSIFEILHIHKGKVK
                     LIFENISFDAGEGDILLVPPKTLHRDSFDIDKGVEILFCSFSWVLSKQYLNIVNIEKV
                     NSTKLKCRNQLGSIFSNIIMDLHGGTEFDMLLTRSRILTILLTILRETVVSQSPATDK
                     KDISHIRRMKIFKQAMDYLNMNYAKCVSLDEIADSINISPYYLSHIFSEESGLTLFSY
                     LTNLRMNKAGEFLKSGKLTISEIAYKVGYENANYFSKVFRKHFNCSPTDFAVQK"
     gene            complement(1519..1791)
                     /locus_tag="PLK08_02005"
     CDS             complement(1519..1791)
                     /locus_tag="PLK08_02005"
                     /inference="COORDINATES: protein motif:HMM:NF036579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPS52302.1"
                     /translation="MGWSGIPAYFYGAYVLGVKPQSDGFRIFHVAPAFHVCSSASGTV
                     PTQHGNIEISWWGADNEFHGKLRHPAAITPKFSEAASTAKWEISQS"
     gene            2429..3202
                     /locus_tag="PLK08_02010"
     CDS             2429..3202
                     /locus_tag="PLK08_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPS52303.1"
                     /translation="MLSNNKNLPYWATGLAIKPDAGPIFRQIKAWAIRAITESLFSPG
                     ELFPSEVQLAKVAGISRLTVRQATNELVAEGLLIREQGKRPRVAMAKTVTHFLELGGI
                     SRYLDSQGKKYYREVLCSEIQACPSEIAKILNLKKHNPIFMLERIRSTEYEKLGWEKT
                     WMNRALCPGIENHDFSVESLYKILISKYGIMPHRADGIIDVAVAGSEHSKMLDVKEGA
                     PLLSVRRTVYTDDNKPLQFNHEIYRGDRYSFAFNVKNLQ"
     gene            3314..3862
                     /locus_tag="PLK08_02015"
     CDS             3314..3862
                     /locus_tag="PLK08_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HPS52304.1"
                     /translation="MIKQSEIKKYQQIAANALQDAGISLTDEERANIEIAELSLGEFE
                     RTGVSLVTYINTDYYCAKELVLLPGQTCPEHRHPSVNGKPGKMETFRCRCGIVYLYIP
                     GKPATQPRAIVPSGSENCYSVFHEIILEPGQQYTIPENTLHWFQAGSKGAIVSEFSTT
                     SRDESDIFTDTRIKRIPEIIEN"
     gene            3938..4498
                     /locus_tag="PLK08_02020"
     CDS             3938..4498
                     /locus_tag="PLK08_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPS52305.1"
                     /translation="MSVRTDHFPSALAENANVNIEIARQAINTSLDYNVDERINRGEM
                     TMQNYFDSHLKGLGKLNYCGFEKAWKQIVGEDFPEIKQAYHDLRVPLYILSNINDVHT
                     QVLKQHWLNDIAVQFWASYEIGLIKPAHEVYEFALKNIDCKPEEILFFDDLIPNIATS
                     LEMGINATLFTCPADVINKFKKYELI"
     gene            4600..5451
                     /locus_tag="PLK08_02025"
     CDS             4600..5451
                     /locus_tag="PLK08_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPS52306.1"
                     /translation="MPLVRMTELLNDARNNRYAIAAFECWGSANIHAISQGAAECGMP
                     VIFQASPTEYGIMGGPEILRKVVETYVNFTGITAALHLDHATTLQQIELCVKAGFTSV
                     MLDASRKHFNENLALSKAAAKIAHQHSICIEAELGHVGGLEGAIEESEHDLDCLTDPD
                     EAIEFVRETGIDCLAVGIGTVHGDYKGEPKINLERLKEISAVIDIPLVLHGGSGTPED
                     IIRKAIRLGISKINICTEIHKSWLEGVALAKKELTPSIPGKFYQPAHNMLKEKVIEII
                     KLFTNIQ"
     gene            complement(5640..5713)
                     /locus_tag="PLK08_02030"
     tRNA            complement(5640..5713)
                     /locus_tag="PLK08_02030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5677..5679),aa:Pro,seq:cgg)
     gene            5910..6551
                     /gene="lipB"
                     /locus_tag="PLK08_02035"
     CDS             5910..6551
                     /gene="lipB"
                     /locus_tag="PLK08_02035"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPS52307.1"
                     /translation="MKTLITEDLGQISYAEALEKQLRLVAGLIENDNEPERLLLLEHV
                     PAVITAGRRGREEDFLVPRERLTELGIELHESSRGGQLTYHGPGQLVGYLIRKLDSHN
                     NTVHDHVWRLEESVIRLLAELGIAADRKKWFTGVWVGEEKITAIGVAVKRWVTYHGFA
                     LNVNTDLDCFKLFVPCGIRDKGVTSIQKILSRQMDMADIKKRLDICLRSVYGY"
     gene            complement(6566..7330)
                     /gene="pgl"
                     /locus_tag="PLK08_02040"
     CDS             complement(6566..7330)
                     /gene="pgl"
                     /locus_tag="PLK08_02040"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPS52308.1"
                     /translation="MDKARTIFIEDSQQAAACRAVEVIESVVHESVASRDVCNIALAG
                     GTTPIPAYQVLATRAMTGSIPWNRVNFFFGDERNVPPDHIESNYHTAQRELFDKAPVD
                     WSRVYPMPGDAADLQAAAVSYEQTIRHVVGDNSGAGIPHFDLIMLGMGCDGHVASLFA
                     GSALLDETKKLVAAEHVEMLGRYRMTMTLPLIKAAHNVLLLVTGEDKAEMVYKAVIEG
                     DRSIPAMHIKPTSGKFWIVVDKAAARLAVDEIEQRH"
     gene            complement(7400..8869)
                     /gene="zwf"
                     /locus_tag="PLK08_02045"
     CDS             complement(7400..8869)
                     /gene="zwf"
                     /locus_tag="PLK08_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPS52309.1"
                     /translation="MTKVTGNVSGQSAVANQFVLFGSTGDLAARKILPALYNLLRDGH
                     LPHGIVLLGVGRKPLCDNDFRRLVEKSVKEFSRQKSPQDDAVLAEFLKHAHYYQLDAH
                     MADEYVGLANRLAELAAAYDMEDNRIFHLSVGPDLFRPIVSGLGAAGLTGQGPERFSR
                     LVVEKPIGHDFQSACDLNAGLDEYFAENQIYRIDHYLGKETVQNILAFRFANALFDPH
                     FNYQWVDNVQITTFETLGMEGRRGPYYEQAGAMGDMMQNHMLQLMSLVAMDRPSCLRC
                     GDIRRKKLEFLKTVRVPADGRTCCAMGQYAGYRDEAGVSPESAVETFAAVRLEVDNLR
                     WQGVPFYLRTGKMLSRRESYIVLEFKAEEMACCKETSSRNRLVMRINPNEGIAMEFLA
                     KRPGMGMVLEPVEMDFSYSVAFASSSSEAYEHLLLDVMLGDQTMFISSDEVQECWRIV
                     DGVRRKFHDMKMSPEIYTAGSDGPKTADEIFSGETHSWI"
     gene            9343..10104
                     /gene="tpiA"
                     /locus_tag="PLK08_02050"
     CDS             9343..10104
                     /gene="tpiA"
                     /locus_tag="PLK08_02050"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPS52310.1"
                     /translation="MSRKLFIAGNWKMNTTAQSGTALAKGLVETIGDISEIETAFFVP
                     ACYLSIISNAVDGSNIGCGAQNMYFEPKGAFTGEISADMVKDCGCKYVLLGHSERRHI
                     FGETDEMINKKVLKALESGLKVILAIGELLSERKAGQTNAVNERQLRKGLANVTAAQM
                     TDVTIAYEPVWAIGTGETATPQQAQEAHAYARGILADMFGRNVSDNVRIQYGGSMNPK
                     NAAELLAQQDVDGGLIGGASLKVDDFTTVVKAGLK"
BASE COUNT         3020 a         2408 c         2236 g         2572 t
ORIGIN      
        1 cttttatatc cactcgcatc tcaggagcgg tatgcgtatg cgtggcatta aaaacaacct
       61 tcatgggatc aaccccattt gcagctttct tcagcctgga acgcacctca tcacgcaatt
      121 catcaggaat acacaccaaa tcgcaactca ccattactgc ctgatcctca ccttcaatgg
      181 ccatagccgt aatagttatg ggatccagca cactttcaga tacgcgagca caaaactgcc
      241 ctgcaataag tactggcata gacggcgtta tatcagcctg accccaaccg attttaacag
      301 tcatttccgg cgtattcatg gtagattcct tttcatatcc tgtggtattt ttacatgaaa
      361 ttatcgcacc agcaatcaac actactacaa ctatcagttc tcttacaatc atggttaagt
      421 cccttcggta tattgttcgt ggttcgcgag tatgactgta acgggctttt cagcttttat
      481 ctcaacaatt gtgattatcg cacagatact aaacccgtca ataataacaa tttgctattg
      541 cgggtaattt attgctttgt tatatctaca tggacctgct tgagatgctt gacaatattt
      601 ttaaaacagt taaattaatc agataatggc ccaaaaaaat gacatcttgt tgaaattcgc
      661 acatgcttac ctgcccgaaa tacgtacatt gggcctgatg cagttcgacc ccatatgggc
      721 tcaacgaatg cacgaaacgt ctattttcga aatactccat attcataaag gcaaggtgaa
      781 gcttatattt gagaatatct ccttcgatgc cggcgaagga gatattctcc ttgtaccgcc
      841 gaaaactcta caccgtgaca gttttgacat agacaaaggc gtcgaaattc ttttttgcag
      901 tttttcatgg gtattgagca aacaatacct gaacatcgtg aatattgaaa aagtcaacag
      961 tacaaagctg aaatgccgaa atcaactcgg cagcattttt agcaatatca ttatggatct
     1021 tcacggcgga acggaattcg acatgttgct gacgcgatcc aggattctaa cgattctgct
     1081 gacaatcctg cgagaaacag ttgtgtcgca atcgcctgcg acggataaaa aagacatttc
     1141 gcatattcgt cgcatgaaaa tattcaaaca ggcgatggat tatctgaaca tgaattacgc
     1201 caaatgcgtt tcgctggatg aaattgccga cagtatcaat atcagcccct attatctctc
     1261 tcacatattc agcgaggaaa gcggattgac tctgttttca tatttgacca acctgcgaat
     1321 gaacaaagcc ggtgaatttc tgaaatccgg caagctgact atttcagaga ttgcctataa
     1381 ggtcggctat gaaaacgcaa attacttttc aaaggttttc cgaaagcatt ttaactgctc
     1441 tccaacagat tttgcagtac agaaatgatt ttgcaataaa taccattcag tggcattttt
     1501 atgcgtttta ttcatttatc atgactgact aatttcccac ttagccgtgg aggcagcttc
     1561 agaaaatttc ggagtaatgg cagcaggatg tctaagtttt ccatgaaact cattatcagc
     1621 cccccaccat gaaatttcaa tattgccatg ctgcgtcgga acagtgccac ttgcgctgct
     1681 gcaaacatga aaagccggtg caacatggaa aatcctgaaa ccatcagatt gtggcttaac
     1741 tcccaaaaca tatgcaccgt aaaaatatgc tggtattccg ctccaaccca agaaaatcca
     1801 catccttttg gttaaattcg gcgtcgcttc cgtaagcgat cgcccgcaat actgccggta
     1861 aagttatcag tgttacttat cccgctaacc gaacaaaaaa ttcgaccgca atgccgaagg
     1921 ccggataatc gcggaatact tccccaacca gacgtcggtg cattaccagt acacgggcgt
     1981 tatatcatat aaagtgggag taaaatttgg ataaattcag cacagcgaag ctacggcctg
     2041 tttatcctta cctagcgcgt aacggactca gaaagcagac cattgttgtc gtaaaagcct
     2101 ttgaactttc acaacagctg aaaataagtg gtttactatt gccgttgtcg caaaacgtct
     2161 gatattggaa attctgtgtt tgaactattg cgacaatggc gtaagtcttg atatggaaat
     2221 aagaaagctc tttgacattc tcgccaaagg gcctttcgtt tcgataagtt ggagcgacag
     2281 gacacggttt agaacttttt gctagggaaa tttcacaatt caaaaactgg ctgcatattt
     2341 tgattaataa gtaaaatttt ttccataatt gcattgaaaa ggcaaaagat gcttgacaag
     2401 aaaatgaaaa ccgctaacat atatatacat gttgagcaat aataaaaacc ttccatactg
     2461 ggccacaggg ctggctataa aaccagacgc aggtccaatt tttcgtcaga taaaagcttg
     2521 ggccatccgg gcaattacgg aaagcctatt ttcgccggga gagctatttc ccagtgaagt
     2581 ccaactggca aaggtggccg gcatttcacg cctgacagta cgtcaagcca caaacgaatt
     2641 agtcgcagaa ggactgctta taagggagca aggtaaacgc ccgcgagtgg cgatggctaa
     2701 gacggtgaca cattttctcg agcttggcgg cataagtcgc tatcttgaca gccaggggaa
     2761 aaagtattac cgcgaggttc tctgctcgga aattcaagcc tgcccgtcgg aaatagcaaa
     2821 aattctaaat ttgaaaaaac acaatccaat ttttatgttg gaaagaatca ggtcaactga
     2881 atatgaaaaa cttggctggg aaaagacatg gatgaaccgg gcactctgtc ctggaattga
     2941 aaaccatgat ttcagcgtgg aatcactcta taaaattctt atttccaaat acggcatcat
     3001 gccgcatcgt gccgacggca taatcgatgt cgcagtagcc ggcagcgagc acagtaaaat
     3061 gctcgacgtg aaagaaggag ccccactact gtcagtacgc agaactgttt atactgatga
     3121 taataaacca ctgcagttca accatgaaat atatcgtgga gacagataca gtttcgcatt
     3181 taacgtaaaa aatttgcaat aacagaagct caaaatggac tttttgtgca ttatcgacca
     3241 gattccgcct ggattatttc tcattagaca agtcaaaggc tgatatatca atttaattta
     3301 aaaagagatg aaaatgataa aacaaagtga aataaaaaaa tatcaacaga tcgcagctaa
     3361 cgctttgcaa gatgccggga tttccctgac ggatgaagaa cgggcaaata tcgagatagc
     3421 ggaactcagc ctgggggagt ttgaaagaac aggtgtatct ttagttactt atataaatac
     3481 tgactattat tgtgccaaag aattagtcct tcttcccggg cagacatgtc cagagcaccg
     3541 ccacccatca gtcaatggaa agcctggaaa aatggagaca ttccgatgtc gatgcgggat
     3601 cgtatacctt tatatcccag gcaagccggc aacgcaaccg agagctattg ttccgagcgg
     3661 tagtgaaaat tgctactccg tattccatga aattatcctt gaacctggcc agcaatatac
     3721 tattcccgaa aatactctgc attggttcca agccggcagt aaaggggcca ttgtttccga
     3781 attttcgaca acaagcagag atgagtcgga tatttttacg gatacaagaa taaaaaggat
     3841 tcctgaaata attgaaaatt aactcagcaa cctgcgacta attcaataaa agtacgatat
     3901 gataaaatct attttaagcg acattggaaa cgttatattg tcagtacgaa cagatcattt
     3961 tccgtccgcc ttggcagaaa atgcgaatgt caatattgaa attgcccgtc aagcaataaa
     4021 tacatctctg gactataatg tcgatgaaag aatcaataga ggggaaatga caatgcagaa
     4081 ttattttgat tctcatttaa aaggcctagg aaagttgaat tattgtggat ttgaaaaggc
     4141 ctggaaacaa atcgtcggag aagattttcc ggaaattaaa caggcatatc atgatttaag
     4201 agtcccatta tatattttaa gtaatatcaa tgacgttcac acccaagttt tgaaacagca
     4261 ctggctaaat gatatagcag ttcaattttg ggcatcatat gaaatcggtc tgattaagcc
     4321 cgctcatgaa gtttatgaat ttgcattaaa aaatatagat tgtaaacccg aagaaatcct
     4381 gtttttcgat gacctcatac ccaacatcgc aacatcgctt gagatgggaa ttaacgctac
     4441 tcttttcaca tgtccagccg atgtgataaa caaatttaaa aaatatgaat taatttaatt
     4501 ctatagaaaa ttacattttg ttttccagat ttattgctta cagaacataa atgcgttaac
     4561 agaatttcat atataatact ttcttttaaa ggaaaaatca tgccattggt gagaatgacc
     4621 gaactactta acgatgcccg caacaatagg tatgccattg cagcattcga atgctggggc
     4681 agtgcaaata tccatgcgat ttctcaagga gccgcagaat gtggaatgcc tgttattttt
     4741 caggcaagcc ctacagaata tggaattatg ggcggtcctg aaattctcag gaaagttgtt
     4801 gaaacctatg tcaatttcac aggcatcaca gccgccctgc atctggacca tgctacaact
     4861 ttgcaacaga tagaactatg cgttaaagcc ggatttactt ctgtaatgct tgatgcatcc
     4921 cgaaaacatt ttaacgaaaa tttagcgtta tcaaaggcgg ctgcaaaaat agctcatcaa
     4981 cacagtattt gcattgaagc agaattaggg cacgttggcg gccttgaagg agccatagaa
     5041 gaaagcgaac atgatttgga ttgtttgacc gatcccgatg aagctataga attcgttcga
     5101 gaaaccggca tcgactgttt agccgttgga ataggaactg tccacggaga ttataaaggc
     5161 gaaccgaaaa tcaacctcga acgcttgaaa gaaatctctg cagtaataga tatacctctg
     5221 gtattgcacg gcggatctgg aactcctgaa gatattattc gaaaagccat ccgtcttggt
     5281 atttcgaaaa ttaatatatg cacagaaatt cataaaagct ggcttgaagg tgttgcattg
     5341 gcaaaaaaag agctgactcc gtcaattcct ggaaaatttt atcaaccagc ccataacatg
     5401 ctcaaagaaa aagtaattga aataattaaa cttttcacca acattcaata agaggcatgc
     5461 caagccatct gtcaactcca tatataaaat cggagcgaca ggatttcgct acgcggcgct
     5521 aaagcgtgaa cctgcggggg cgtccgttcg cttcgctcac atccgcagcc gcagattcaa
     5581 ttttgccctt cggccaataa aaaagaccaa cccattaaag ggctggtctt ttttaccagt
     5641 cggagcgaca ggatttgaac ctgcggcctc tgcgtcccga acgcagcgct ctaccaagct
     5701 gagctacgct ccgaattgca cacataaacg gcgaacagag gacagaattt tatccatatt
     5761 caactataaa tcaagtatta tccctctatt atactgcaaa aaatacctca ggccgtttca
     5821 aaacctgctt tccccataag attgaaagat ttcacggcca atcgtagccc aaccttgaat
     5881 acatcggctg aaatacgtac actgcggata tgaaaacgct gataacagaa gacctcggcc
     5941 aaatcagcta tgccgaggca ttggaaaaac agctcagact cgtcgccgga ttaatcgaaa
     6001 acgataatga gcctgagcgt ctgctgctgc tggaacacgt accggctgtc ataaccgccg
     6061 gcagacgcgg cagggaagag gattttctcg tcccacgcga gcggctgact gaacttggaa
     6121 tagaattgca cgaatcaagt cgcggcggac aacttaccta tcacggccct ggccaactcg
     6181 tcggatatct catacgaaaa ctcgattcgc acaacaatac ggtacacgat cacgtgtgga
     6241 gacttgagga gagcgtaata aggctattgg cggagcttgg catagctgcg gacagaaaaa
     6301 agtggttcac gggcgtatgg gtcggcgaag aaaaaatcac ggctatcgga gtcgccgtca
     6361 aacgctgggt gacttatcac ggcttcgctc tgaacgttaa taccgacctg gactgcttca
     6421 aactctttgt tccgtgcggc attcgcgaca agggtgtgac cagtattcaa aaaattctca
     6481 gccgacaaat ggacatggct gacatcaaaa aacgcctcga tatatgcctc agaagcgttt
     6541 acggctatta aaagacgtca tctattcagt gtctctgttc gatttcatcg accgccagtc
     6601 gagcggcggc tttatcgacg acaatccaga atttgccgct ggtcggtttt atatgcattg
     6661 cggggataga gcggtcgcct tcgatgacgg ctttgtagac catttcagcc ttgtcttcgc
     6721 cggtaacgag cagcagtaca ttatgagccg cctttatgag cggcagagtc attgtcatac
     6781 gatagcggcc gagcatttcg acgtgttccg cagctacaag tttttttgtt tcatcgagaa
     6841 gtgccgagcc ggcaaaaaga ctcgccacat ggccgtcgca gcccatgccc aacattataa
     6901 ggtcaaaatg cggtatgccg gctccgctgt tgtcgccgac gacatggcga atggtttgtt
     6961 catagctcac ggcagcagct tgaaggtcgg cggcatcgcc gggcatgggg tagacgcgag
     7021 accagtctac gggggctttg tcaaaaagtt cacgctgagc ggtgtggtaa ttgctttcta
     7081 tatggtcggg cggtacgtta cgctcgtcgc cgaagaagaa attgacccga ttccacggaa
     7141 tcgaccctgt catggctctg gttgcaagaa cctggtatgc cggtatcggc gtcgtaccgc
     7201 cggcgagcgc aatgttgcac acgtcgcgag acgcgactga ctcatgcacc accgattcta
     7261 taacctccac agccctgcac gccgcagcct gctgcgaatc ctcgataaat attgtcctag
     7321 ccttgtccat ccaatcactc cccatccgtt cgagtttttt tacattctat gtcgtttaca
     7381 gctaaaatgc cacaaaaagt tatatccagc tgtgcgtttc tccggaaaaa atttcatccg
     7441 ccgttttcgg gccgtcgctg ccggcggtat aaatctccgg cgacatcttc atatcatgaa
     7501 atttccttct tacgccgtcc actatccgcc agcattcctg aacctcgtct gaactgataa
     7561 acatggtctg gtcgcccagc atcacgtcca gtagcagatg ttcatacgcc tccgaagacg
     7621 acgacgcaaa agccaccgaa tatgaaaaat ccatctcgac aggttcgagc accataccca
     7681 tgccgggccg cttagccaga aattccatcg caatgccttc gttgggattt attcgcatga
     7741 ccagccggtt gcgactagat gtctccttgc agcaggccat ctcttcagcc ttgaattcca
     7801 gaactatata actttcccgc cgcgagagca tcttgccggt acgcagataa aacggcacgc
     7861 cctgccagcg gaggttatcc acttccagcc gcacagcggc gaacgtttca acggcggatt
     7921 caggcgatac gcctgcttca tcgcgatagc cggcgtattg tcccatcgcg caacatgtcc
     7981 tgccgtcagc cggcacacgt accgttttca gaaattcgag tttttttcgc cgtatatcgc
     8041 cgcatcgcag gcaggacggc ctgtccatcg ccacaagcga catcagttgc aacatgtgat
     8101 tctgcatcat atcgcccata gcgccagcct gttcatagta aggcccgcga cggccctcca
     8161 tgccgagcgt ttcaaacgtg gttatctgca cattatcgac ccattgataa ttgaaatgcg
     8221 ggtcgaacag cgcattggcg aaacggaacg ccaaaatatt ctgcacagtc tccttgccga
     8281 ggtagtggtc tatacgatat atctgattct cggcaaaata ctcatccaga ccggcgttaa
     8341 ggtcacaggc ggattgaaaa tcatggccga tgggtttttc cacaaccagc cgcgaaaacc
     8401 gctcgggccc ctgacccgtc aaaccggcag ctcctaaacc gctgacgatt ggcctgaaaa
     8461 ggtccggccc gacggacaga tgaaaaattc tgttatcttc catgtcataa gcggcagcta
     8521 actccgccag acgattagcc aggccgacat attcatcggc catatgagca tcaagttggt
     8581 aataatgcgc atgtttcaga aactcagcca atacggcatc atcctgcggc gatttctggc
     8641 gagagaattc ttttaccgat ttctccacca ggcggcggaa gtcgttgtcg cacaaaggct
     8701 tcctgccgac gcccagcaga acaataccgt gcggcaagtg cccatcccgt aaaaggttat
     8761 atagtgcagg cagtattttc cttgcggcaa ggtcgccagt gctaccgaac agcacaaact
     8821 ggttggctac cgcagattgg ccgctgacgt taccggtcac ttttgtcata attagctgtc
     8881 ctttcaggaa attatataca tatttcggcg gcttttccgg aaaaagacaa acaatatgaa
     8941 cctatcccga aaaccttggc tgcctgtaat cggcgacgcc catgtttttg ggataggttc
     9001 tagtcaaaac tggcgacagt atcgaaaaaa cggataagca tgcaaaagct ggtttcaaaa
     9061 cccccaattt cccaaaaaaa tcttgacgta aaagccttaa atcggtacaa atagctctct
     9121 tatgctgcta gaggtatgcg ctgattccgt ataccgacag gcagattagt ataaacttag
     9181 taattctaca gtttaaaatc gtttaaaaat aaccgtcata cggcggacag acgcttaagc
     9241 ctgataaata tgatgtgacg ggttttttaa cctgtcaaag ttttttttat ccgttttgaa
     9301 tttttcgaca gttcgacagt ttgacagaaa gtgaaggtaa cgatgtcaag aaagctgttt
     9361 atagccggca attggaaaat gaacaccacc gcccaaagcg gcacagccct cgcaaaggga
     9421 ctggtcgaaa ccataggcga cataagcgaa atcgaaaccg catttttcgt tccagcctgc
     9481 tatctctcga taatcagcaa cgccgtcgac ggcagtaaca tcggctgcgg tgcacagaac
     9541 atgtatttcg aacccaaagg tgcatttacc ggcgaaatat cggctgacat ggtaaaagac
     9601 tgcggctgca aatacgtgct gctgggccat tccgaacggc gacatatttt tggcgaaacc
     9661 gacgaaatga taaacaaaaa agtcctcaaa gccctcgaaa gcggcttgaa ggtaatttta
     9721 gccatcggcg aacttctttc tgaacgtaaa gccggccaaa ccaacgccgt caatgaacgt
     9781 cagctccgca agggcctcgc caacgtcacc gccgcccaga tgactgacgt tacaatagca
     9841 tatgaacctg tatgggccat cggcacaggc gaaaccgcta cgccgcagca ggcacaggaa
     9901 gctcacgcat acgcacgcgg catattggcg gatatgttcg gcaggaatgt ttcagataat
     9961 gtgcgtatac aatacggcgg ctcgatgaac ccaaaaaatg ccgccgaact gctggctcaa
    10021 caggacgtgg acggcggact catcggcggt gcgtcgctga aagtggacga ttttaccaca
    10081 gtagtcaaag ccggcttgaa atagagaatg acgtcgggtt tttgcccata aaaattctcg
    10141 ccgagtcaat aaaaaggtta aaaagacagg aacaaggtaa tgggaacttt tcttctgata
    10201 tcgttgctga tagtggcagt atttcttatt ctggtc
//
LOCUS       DAONFI010000036        10198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7466_length_10198_cov_3.706694, whole genome shotgun sequence.
ACCESSION   DAONFI010000036 DAONFI010000000
VERSION     DAONFI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_10198_cov_3.706694"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /gene="hcp"
                     /locus_tag="PLK08_02055"
                     /gene_synonym="priS"
     CDS             complement(<1..1223)
                     /gene="hcp"
                     /locus_tag="PLK08_02055"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPS52311.1"
                     /translation="MDMFCFQCEQTAGGRGCTKHGVCGKDPAVAALQDLLVYAAKAIA
                     MYARRARDLGASSHDVNIFTLEALFTTVTNVNFDARCLVTRLKKAGEILQKAKSLYEY
                     AADKAGVTPENLDDEIAFDPSDDLEDMIEQSRRIGIESRRTFINNDDILGLQELLTYG
                     LKGMAAYADHAMILGMEDQSVYAFIHEALDFLTGPKNKNVNELVNMCLRCGEMNLKTM
                     ALLDAANTEAYGHPEPTQVRITPVKGKAILVSGHDLKDLAMLLDQTQDTGIKVYTHGE
                     MLPCHGYPLLNQYPHLVGNYGGAWQDQQKEFAEFPGAILMTTNCIQRPAECYKDRIFT
                     TGLVAWPGVKHINNSDFTPVIEAALAAEGFVEDAPEKSIMVGFGHAAVMKLAPMLIES
                     VKSGGIRHFFLIGGC"
     gene            complement(1446..2750)
                     /locus_tag="PLK08_02060"
     CDS             complement(1446..2750)
                     /locus_tag="PLK08_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPS52312.1"
                     /translation="MDVYQIIAIVVVLTAVLNHVNRRVLKLHPTIGMMLAGALVAVAV
                     AVVGQFRHPLAVNVRHWVRELNFNELVLQGILGFLLFAGSLKINLSRLLKRIWPIGIF
                     AVGGVVFSAFIIAWILNAVSQCCNLGLSFMDCLLFGAIISPTDPIAVLSILKQVGAPQ
                     NLEMDIEGESLFNDGIGVVMFLVVVRVMTSSKGVSVGNVLGLFGLEAVGGILLGWLYG
                     VIASRMIRNNDDVRTQILLTLSIATGGYQFADVIHASGPLAMVVAGIVVGNDKGCMRC
                     EVRSHLDNFWDVVDEVGNAILFMLVGLEMLLVANEALPEWRICLLAAAVTVPAVLVSR
                     YFSVLIPYLGLKWKFPFSRGTLAIMTWGGLRGGLPLAMALCIPTTELQISRYHITHAE
                     TLDRAMLLMMTYSVVIFSILVQGLTIKPLVRFFKNPAADDCE"
     gene            3155..3880
                     /locus_tag="PLK08_02065"
     CDS             3155..3880
                     /locus_tag="PLK08_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPS52313.1"
                     /translation="MEMQTVQNFNITRPETLVGNIARSLIGFIAASGMGDGDKLPSER
                     QLVEQMDASRLPLREALSMLKGLGIVQAHHGKGVFVKQVELKNIFKMLSPLLKVQRNI
                     SMDDIMVVRYYLEPAIAAQAAARRTDSDIRKMQEHLKAMQDNVAEIEKFIIPDIAFHV
                     TLAQATGNMIFDLFVSVMHDLIVQVQKSFPDQEASRKKSVQFHADILRAIIERDEKTA
                     AEKMRQHIEDIGKAICKTLKSSR"
     gene            3853..4842
                     /locus_tag="PLK08_02070"
     CDS             3853..4842
                     /locus_tag="PLK08_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HPS52314.1"
                     /translation="MQDAKKFKVIVTDYVFESFENEEKSLAEIGATLELHQCKSQNEL
                     TAVIKDADVILNTYLPGLDAKVFSYAPKLKGIVRYGIGLDTISISDATAAGIKVANVP
                     DYCVEEVADHAMSLFLCMARKVALSDKKMKSGVVSLGYVKPLKGITSMTAGIIGFGRI
                     GQAIASRLKPFGSKIVFSDPCIDDSKGLAEKVSLDELCETCDAIFVQCPANKHTHHLL
                     DASRFASMRKSPIIINAARGQIIDTAALLDALQSGKASAAGLDVLEDEKQVIESDHPF
                     RKMDNVILTPHSAWYSSDAIIKLQTQACQEVVRILKGENPKNFANPEVLENRK"
     gene            4839..5627
                     /locus_tag="PLK08_02075"
     CDS             4839..5627
                     /locus_tag="PLK08_02075"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPS52315.1"
                     /translation="MKIAKIKFDGKTSYAKVEDNGYNLIGDKIFGQYAATGTIIPFDK
                     AKLLVPVEPWQIIAIGLNYRQHAIECNMQFPKAPVVFIKTPNTITGPGSDIILPKMAP
                     DCVDYEAELAIIIGRQAKNVTENEAGDYILGYTCSNDVSARDCQLKQDVQWARGKCFD
                     TFAPIGPYIVTGLNGDNLDIKLTLNGQVMQQSNTSDLIFSCKTLVSYISRCMTLYPGS
                     IIMTGTPSGVGMGRNPQVYMKHGDKVCVEIEGIGCLENTVVTEA"
     gene            5629..7107
                     /locus_tag="PLK08_02080"
     CDS             5629..7107
                     /locus_tag="PLK08_02080"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPS52316.1"
                     /translation="MNLLTEKFYIDGKWVESDSGKTVTIINPANGQILANVHQASKEQ
                     IKQAIAAADKAFKETWGPMPAIQRSRIMHKIANLINENIEGLSAIETNNVGKPIIESR
                     NVDIPGAADTFEFFANVSVDILGNTIPSPFGNAVMDYTIREPLGVIGAITPWNFPLLL
                     AARKIATSIAAGNTIVIKPASLTPLSTLALGKIFDAAGLPAGVVNIVTASGRDIGEAF
                     ASSTTIHEVTFTGSTSTGTELMRQCASSRIPCTLELGGKSPAVICEDCDVEEAVAGTL
                     FGVFINQGECCCGLTRLIVHQNVYDEFTSKFVSAAKKIRVGNPMDEKTQMGPVVDAGH
                     LETVMEYIDSGKSQGATLACGGIRLAGDEFAKGFFVEPTVFTNVTPDMRIWREEIFGP
                     VVTITKAKDNDELIELANDTDYGLAASIWTKNLKTAHWMSAKIEAGTVWFNLHNFLFP
                     AAPYCGFKASGVGGELGKQSLIAMTKTKNVMINLMQDTFKWY"
     gene            7152..9227
                     /locus_tag="PLK08_02085"
     CDS             7152..9227
                     /locus_tag="PLK08_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPS52317.1"
                     /translation="MSTDRINMLRQRCFSRKIQQFRYQLIAAAKSLKETENIESMDIR
                     FGLCTKHVLSSLKFDMDELELLAGRLVCQQIEADKEAFAKAVEYINDNLPTGGQTGHC
                     ELDISKLFQYGIDGLTEYIKKFEQNDARDSFVYALEGLSAMIENIAAMVSAKISDASA
                     ARKSELQIIAESCKNIAHNPPCNFRDAIQLLWLVDIGVMFGENVSLVVPGHLDRTLWP
                     YYKHDIDAGIITRQDALTLIESLYILINEAIPDGLAMSVMVGGTNAYGQDVTNELSYI
                     CMEAVRKTKMIYPTVGVCWNEQTPDALVNLTVGLIAEGYKTPAFFGDRTIQKGLESYG
                     LNKEERCNYINSTCVEITPVGASNVWVASPYFNLCQWLLDEIKQTESKEPDDFADFLN
                     RYFMRASKKIGEAVEQQNQLRKLRYQNSGKPLQSVFTRDCIKNGKNIDRGGASYNWVE
                     CSLVGIANLADSLTVIKHEIFEKHNLAFSDLTGLLEKNFSDNEPVRLRFLNSYPKYGQ
                     ADEEVDLLVKTIAQYLIGECKKHKMFPDDSPFMPGMFCWIMHEHLGGQTGATPDGRKA
                     GFPFADGGGPAQGRETCGPTAGIISTTCWNHSPLIGGLAYNMKFSSQLFKTDKGKESL
                     KDLILTFLRRGGFETQINVIDRETLLKAQKHPEEYRDLVVRIGGYCDYFTKLSPQMQA
                     EVLLRTEFK"
     gene            9243..10151
                     /locus_tag="PLK08_02090"
     CDS             9243..10151
                     /locus_tag="PLK08_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPS52318.1"
                     /translation="MDSNRSIQGKIFDIQRFSINDGPGIRTTVFFKGCPLNCVWCHNP
                     ESISSTSVLSFLPDKCIGCGYCFKVCPHGAHLMIDGKHVIDRKKCDACGLCAKECYSG
                     ALELVGKIVTVEEVMATVMVDISFYQNSGGGLTVSGGEPLAQPEFCHVLLQKAKAEGL
                     HCCCDTCGHAKWETFKKILNLVDLFLFDVKDTNPERHKINTGVDNKLIQANLQKLHDA
                     GANIWIRIPLIPGWNDFEDNLAGLVEMFKKLPNIKAVEIMPYHKLGISKLERFGLKPR
                     MIINSEPPSKKSIDRLAKLFSFYSGS"
BASE COUNT         2975 a         2330 c         2377 g         2516 t
ORIGIN      
        1 tcgcatccgc cgatgaggaa gaagtgacga atcccgccgc tcttgaccga ctctataagc
       61 atgggtgcaa gcttcatgac cgcggcgtgt ccgaatccta ccattattga tttttccgga
      121 gcatcttcca caaaaccttc cgccgcaagt gcagcctcta taaccggcgt aaaatcgctg
      181 ttgtttatgt gcttgactcc cggccatgcg acaaggccag tcgtgaatat gcggtcttta
      241 taacactcag ccggccgctg aatgcaattc gtagtcatca gaatagcgcc aggaaattcg
      301 gcaaactcct tctgctggtc ctgccacgct ccgccgtaat tgccaaccag gtgcggatac
      361 tgattcagca gcggatatcc gtggcacggc agcatttcgc cgtgggtata aacttttatg
      421 cccgtatcct gcgtctggtc gagcagcatg gccaaatctt tcaaatcgtg gccggataca
      481 agaattgctt tacctttgac gggcgtgata cggacctgag tcggctcagg atggccgtat
      541 gcctcggtat tggcagcatc aagaagagcc attgttttca gattcatttc gccgcaccgc
      601 agacacatat ttacaagctc gttgacattc ttatttttgg ggccggtcag aaaatccagc
      661 gcttcatgta taaacgcata tacactctgg tcttccatgc cgagaatcat ggcgtggtcg
      721 gcatatgcgg ccatcccctt caagccgtac gtcagaagct cctgcaatcc caatatgtca
      781 tcattgttga taaacgtccg tcgagactcg atgcctattc gccggctctg ctcaatcata
      841 tcttcaaggt cgtcgcttgg gtcaaatgcg atttcgtcgt ccagattctc cggcgttacg
      901 ccagccttat cggctgcgta ttcatagaga ctttttgcct tttgcagaat ttctccggct
      961 ttcttgagcc gcgtaacaag gcatcgtgca tcaaagttaa cattggtcac agtcgtaaaa
     1021 agcgcttcca gcgtaaatat attcacatcg tgactcgacg caccgaggtc acgcgcacgt
     1081 cgtgcgtaca ttgcgatagc cttggcggca tacaccagca aatcctgcaa cgccgcaacc
     1141 gcagggtctt tcccacaaac gccatgtttc gtacaccccc tgccgccggc agtctgctca
     1201 cattggaaac agaacatatc catgtcaatc tccttttaaa acgaattata gccgcaatgg
     1261 ccaattcctt tttgaaattc taggcatctg taagtatatt tttcagagtt attgaagaaa
     1321 gtgtgtacat ttttccaaaa acgtcccctg aatgatgaac aagcacggcc gaaaaccgcc
     1381 tctcaacgaa taaatactat tcttgaaagg ctataaagat tattttggct gcatgcgtta
     1441 atgactcatt cacagtcgtc ggctgccgga tttttaaaaa atctcaccaa cggcttgatt
     1501 gtcaacccct ggacaagtat tgaaaatata acgacggaat aagtcatcat gagcagcatg
     1561 gctctgtcga gcgtttctgc atgagtgatg tgatacctgc taatctgcag ttctgtcgtc
     1621 ggaatgcaca acgccatagc caacggcaaa ccgccgcgca agccgcccca cgtcatgata
     1681 gccaacgttc cccgtgaaaa cgggaatttc catttcagcc ccagatacgg aatcaaaacg
     1741 gaaaaatatc gtgaaaccag tacggcaggc acggtaacag ctgccgccag cagacatatt
     1801 cgccattccg gcagcgcttc attcgcaacc aggagcattt caaggccgac gagcatgaaa
     1861 agtatcgcgt tgccgacttc atcgacaaca tcccagaaat tgtcaaggtg actgcgtact
     1921 tcacacctca tgcagccttt gtcattaccg acgactatgc ctgcaacaac catcgctagc
     1981 ggccccgatg cgtgtattac gtcggcaaac tgataaccgc cggtagctat gctgagcgta
     2041 aggagaatct gcgtacggac atcgtcatta ttgcgtatca tccgcgacgc gataacgcca
     2101 tacagccatc ccaacaatat cccgcctaca gcctccagcc cgaaaagtcc aagtacattg
     2161 ccgacactca cacctttact ggacgtcatt acacggacaa ctacaaggaa catcaccacg
     2221 ccgatgccgt cgttaaaaag cgactcgcct tcgatatcca tctccagatt ctgcggcgcg
     2281 ccgacctgtt tcaatatgga caatacggcg atgggatctg tcggcgatat tatcgcgccg
     2341 aagagcaggc agtccatgaa cgataacccc agattacagc attgcgaaac cgcattaagt
     2401 atccaggcga tgatgaacgc cgaaaaaaca acacccccga cggcaaaaat tcctatgggc
     2461 cagattcgtt taagcagccg cgaaagattt attttcagcg aaccggcaaa aagcagaaat
     2521 cccaaaatgc cttgaagtac cagttcatta aaattcagtt cacgcaccca gtgtctgacg
     2581 tttaccgcca aaggatgtct gaattgaccc acgacggcaa ccgcaacagc caccagagcg
     2641 ccagccaaca tcatgcctat cgtcgggtgc aacttcaaaa cccgccgatt gacgtgattc
     2701 agcacggctg taagcacaac tacaatcgcg attatctgat atacatccat aatattagcc
     2761 tcataaatac cttcaggata tgtggaaagc aagattttta ttgttcggga agaaaacgct
     2821 tacgtcccgt aacttccaac aattccatga catatgacat gattccagat tcatggagca
     2881 ttttcaaaaa tacgaacgtc aaccataact tcataaattc gcaggcctgg cagtttagag
     2941 gcttgcaaaa cggtactgca acgcagcata cagtgtatag ccggcaaatt tttaccggca
     3001 aaccgattta ctgaaaattg catgaaacat aagcgaatgg tcaatgcgac acattggaga
     3061 aattttacat ggatgaattt atggcggctt gacttattgg aaaattgtgg tatcctacca
     3121 actggtcata tgagtatatc actttggaag aagcatggaa atgcagactg ttcaaaattt
     3181 caatattacg cggcctgaaa cactggtggg caacattgcc cgcagtctta tcggctttat
     3241 tgcagctagc ggcatgggcg acggcgataa actgccgtcg gaacgtcaac tcgtcgagca
     3301 gatggacgcc tctcgtctgc cgttgcggga agcgctttcc atgctcaagg gattgggtat
     3361 agttcaggct catcatggaa aaggcgtatt cgtcaagcag gttgaactga aaaatatttt
     3421 caaaatgctc tcgccgcttc tgaaggtgca gcggaacatt agcatggacg atataatggt
     3481 tgtgcgatac tacctcgaac cggccatcgc cgcccaggct gccgcacgac gtacggatag
     3541 tgacatccga aaaatgcagg aacacctgaa agctatgcag gataatgtcg ctgagattga
     3601 aaaattcatc attcctgaca ttgcttttca cgttaccctg gctcaagcga caggaaacat
     3661 gatatttgac cttttcgtct ccgtcatgca tgacctcatc gtgcaggtgc agaaaagttt
     3721 tccggaccag gaagcgtcga gaaaaaaatc cgttcagttt catgctgata ttctgcgggc
     3781 aattatcgaa cgggatgaaa aaaccgcagc tgaaaaaatg agacaacata tagaagatat
     3841 aggaaaagca atatgcaaga cgctaaaaag ttcaaggtaa tcgtcacgga ttacgtattc
     3901 gaatcgttcg aaaatgaaga aaagtcgctc gccgaaatcg gagcgacgct ggaacttcat
     3961 caatgcaaat cgcagaatga gttgacggct gtcattaaag atgccgacgt gattttaaac
     4021 acatatttgc ctggattgga tgcgaaagtg ttttcatacg ctccgaaact caaaggcatt
     4081 gtgcgatacg gcatcgggct tgatacgata agcatttcgg atgctacggc ggctggaatc
     4141 aaggtcgcca atgtgccgga ctactgcgta gaggaggttg ccgaccatgc gatgtcgctg
     4201 tttttgtgta tggcacgcaa agttgcgctt tcagataaaa agatgaaatc cggtgtggta
     4261 tcgttgggat atgtcaaacc gctcaaggga ataacatcca tgacggcggg cataatcggt
     4321 ttcggccgaa taggccaggc gattgcctca cgattaaaac cattcggttc gaagatagtt
     4381 ttttccgacc cgtgcattga tgactctaaa ggactggcgg aaaaggtatc actcgatgaa
     4441 ctctgcgaaa catgtgatgc catattcgta caatgtcccg ctaacaagca tacgcatcat
     4501 ctgctcgacg caagccgttt tgcatctatg aggaaatcgc cgattataat aaacgcggcg
     4561 cgaggacaga ttatagacac cgcagcactg cttgacgcgt tacagtcagg caaggcatct
     4621 gccgccggtc ttgacgtgct ggaagatgaa aagcaggtca ttgaatctga ccatccgttc
     4681 agaaaaatgg acaacgtgat attaacgccg cacagtgcat ggtattcgag tgatgctata
     4741 atcaagcttc aaacgcaggc ctgccaggaa gttgtaagaa ttttaaaagg tgagaatcct
     4801 aaaaactttg caaacccgga agtattggaa aacagaaaat gaaaatagca aaaatcaaat
     4861 ttgatggcaa aaccagttat gcgaaagtag aagacaacgg atataaccta atcggcgata
     4921 aaatcttcgg acaatacgcc gctactggta cgattatacc tttcgacaag gcaaaacttc
     4981 ttgttccagt cgagccatgg cagattatcg ccattggcct gaattacaga cagcatgcga
     5041 ttgaatgtaa catgcagttt ccaaaagcgc ctgtagtttt cattaaaacg ccaaatacca
     5101 taaccgggcc gggcagtgat attattttgc cgaaaatggc gccggattgc gtcgactatg
     5161 aagctgagct tgccataatt atcggcagac aggcaaagaa cgtgactgaa aatgaggctg
     5221 gcgattacat attaggttat acatgctcaa atgacgtcag tgcgagagat tgccagctca
     5281 agcaggacgt tcaatgggcg aggggaaaat gcttcgacac tttcgcgccg ataggcccgt
     5341 atattgttac cggcctgaat ggcgataatc tggacataaa acttacgctc aacggacagg
     5401 tcatgcagca atctaacaca tcggacctga tattcagttg caaaacactg gtaagctata
     5461 tttcgcgatg catgacgctt tatcctggca gcatcataat gacaggcacg ccgtccggcg
     5521 tgggtatggg ccgtaatccg caagtttata tgaaacacgg cgacaaggtc tgcgtagaaa
     5581 tagaaggcat aggttgcctt gaaaataccg tcgtaacgga ggcatgaaat gaatttgtta
     5641 acggaaaaat tttatattga cggcaaatgg gtagaatcgg attcgggcaa gacggtaacg
     5701 ataataaatc cggctaacgg acaaattttg gcaaatgttc accaggcatc caaagaacag
     5761 attaagcagg ctatcgcggc agccgataaa gcgtttaagg aaacatgggg accaatgccc
     5821 gccatccagc gcagccgtat aatgcacaaa atcgcaaatc tgattaatga aaatatagag
     5881 ggactctcgg ctatagagac taataacgtc ggtaaaccca ttatcgaatc acgaaacgtc
     5941 gatataccag gagcggcaga tacttttgaa ttttttgcaa atgtcagcgt ggatattctc
     6001 ggcaatacga taccctctcc gttcggcaac gctgtcatgg attatacgat acgtgaaccg
     6061 cttggcgtaa ttggcgcaat aacgccgtgg aattttccgt tgctgcttgc agcccgtaaa
     6121 atcgctacct caatcgccgc tggaaatact attgtaatta aaccagcctc tcttaccccg
     6181 ttaagtacat tggcattagg caaaattttt gatgcagccg gcttgccagc cggcgtagtt
     6241 aacatcgtaa cggcatcggg acgtgacata ggcgaagctt ttgcaagttc gacgacgatt
     6301 catgaagtta catttaccgg ttcaacatcg accggaacgg aacttatgcg tcaatgtgca
     6361 tcttcgcgca taccttgcac tcttgaactc ggcgggaaaa gtccggcggt catctgcgag
     6421 gactgtgacg tcgaagaagc cgttgccgga acattgttcg gcgtattcat aaatcagggc
     6481 gaatgctgct gtgggcttac tcggctgatt gtgcatcaaa atgtatatga tgaattcacg
     6541 tcaaaatttg tctcagccgc caaaaaaatc cgcgtcggca atccgatgga tgaaaagaca
     6601 caaatgggcc ccgttgttga tgccggacat cttgaaacag tcatggagta catagattcc
     6661 ggaaaatcgc aaggcgcaac gcttgcctgt ggaggaatcc ggctggccgg cgatgaattt
     6721 gcaaaagggt tttttgttga gccgacggtt tttacaaatg ttacacccga catgcgaata
     6781 tggcgtgaag aaattttcgg ccctgtcgtt acgataacaa aagccaaaga caatgatgaa
     6841 ttgatagaac tcgcgaatga tacagattat ggtcttgcgg caagtatctg gacaaaaaat
     6901 ttaaaaacgg cacattggat gtccgcaaaa atagaggcag gaaccgtctg gttcaatttg
     6961 cataattttc tgtttcctgc cgccccttat tgcggattca aggctagtgg tgtcggaggc
     7021 gagcttggca agcaaagcct catagccatg actaaaacca aaaatgtcat gattaacctg
     7081 atgcaggata catttaaatg gtattgatac atttggtata attggttttt gatttgagga
     7141 taactaataa aatgtccact gacagaatca atatgctcag gcagcgttgt tttagtcgta
     7201 aaatacaaca gttcagatat cagctgattg ctgccgcaaa atcactaaaa gaaactgaaa
     7261 acattgaatc catggatatc cgtttcggtt tgtgtacgaa gcatgtgctt tcgtctctga
     7321 aatttgacat ggatgaactg gaattattgg ccggccgcct tgtatgtcag caaatagaag
     7381 cagataaaga agctttcgcc aaagctgtag aatatataaa tgataatctt cccacaggcg
     7441 gccagacagg acattgcgag ctggatatct caaaactttt tcagtacggc attgacggat
     7501 tgactgaata tataaaaaaa tttgaacaaa atgatgctcg agattcattt gtatatgccc
     7561 tggaaggttt atcggccatg attgaaaata ttgcggctat ggtcagtgcg aaaatttccg
     7621 acgcatctgc cgcaagaaaa agcgagcttc aaataattgc cgaatcgtgt aaaaatatcg
     7681 cacataatcc gccctgtaat ttcagggatg caattcagtt gctttggctt gtagatatcg
     7741 gcgtcatgtt tggtgaaaat gtcagcctgg tcgtaccagg acatctggac aggactcttt
     7801 ggccgtatta taaacatgat atagatgccg gcataatcac acgacaggat gcgttgacgc
     7861 ttatcgagtc tttatatatt ctgattaacg aggctattcc tgacgggttg gccatgtccg
     7921 taatggtcgg tggcacaaat gcatatggac aggacgttac gaacgaactt tcatatatat
     7981 gcatggaagc agtaagaaaa acaaagatga tatatccgac agtcggcgtt tgctggaatg
     8041 aacagacgcc tgacgcactt gtaaatctta cggtcggcct tatcgccgaa ggttataaaa
     8101 cgccggcgtt ttttggcgac agaaccattc aaaaaggtct ggaaagttat ggtttgaaca
     8161 aagaggaacg ctgcaattac ataaattcca cgtgcgtaga aataactcct gtgggggcga
     8221 gtaacgtatg ggtggcttcg ccgtatttta atctgtgtca atggctgttg gatgaaataa
     8281 aacagaccga atcgaaagaa ccggatgatt ttgctgattt cctcaatcgc tatttcatgc
     8341 gagccagtaa aaaaatcggc gaagcggttg aacaacagaa ccagttgcgg aaattacgtt
     8401 atcagaactc cggcaaacca ctccaaagcg tctttacacg agattgcata aaaaacggga
     8461 aaaatatcga ccgtggcgga gcatcgtata attgggtcga atgttcatta gtaggcatag
     8521 ctaatctggc ggattcatta accgttatca agcatgaaat ttttgagaaa cacaatttag
     8581 ccttcagcga tttaacagga cttcttgaaa agaatttttc cgataatgaa cctgtcaggt
     8641 tgcgtttttt aaattcatat cccaagtacg gccaagctga tgaagaagtg gatttattgg
     8701 taaagactat tgcgcaatat ctgataggcg aatgtaaaaa acataaaatg tttccggacg
     8761 acagcccgtt tatgcctgga atgttctgtt ggataatgca tgagcatctt ggcggccaga
     8821 caggtgctac gcctgatggg cgcaaagccg gtttcccgtt tgcggatggc ggcgggccgg
     8881 cgcagggacg cgaaacatgc ggcccgacgg ctggcattat ttcaacgaca tgctggaatc
     8941 actcccctct tatcggcggc ctggcgtata atatgaaatt ttccagtcag ctttttaaaa
     9001 ctgacaaagg caaagaatcg ctcaaagatc ttattttaac atttctccgc aggggaggat
     9061 ttgagaccca gataaatgtc attgaccgtg aaactctttt aaaagcccag aaacatccgg
     9121 aagagtatcg cgatctcgtc gttcgaatcg gcggatattg tgactatttc acaaagctct
     9181 cgccacagat gcaggctgaa gtgctgttgc gaacggaatt caaatagcag gttatacaaa
     9241 agatggacag taatcgttca attcagggaa aaatttttga catccagcga ttttccatca
     9301 acgatggccc tggaatacgg actacggtgt tctttaaagg ctgcccgctg aattgcgtat
     9361 ggtgtcataa ccctgaaagc ataagttcaa cgtcggtact ttcttttttg cctgataaat
     9421 gcatcggctg tggatactgt ttcaaagtct gcccacatgg cgcacatctc atgatagacg
     9481 gtaaacatgt aatcgaccgc aaaaaatgcg acgcctgtgg cctctgtgct aaggaatgtt
     9541 attcaggggc actcgaactg gtcgggaaaa tcgtcacagt ggaagaagtt atggcaacgg
     9601 ttatggtgga tatatctttt tatcaaaact ccggcggcgg cctgactgta tctggcggcg
     9661 aaccattggc ccagcccgaa ttctgccatg tactgctgca aaaagcgaaa gcagaaggac
     9721 ttcactgctg ctgcgataca tgcggacatg ccaaatggga aactttcaaa aaaatcctca
     9781 atcttgtcga tttattttta tttgatgtaa aagacaccaa tccggagcgg cataaaatta
     9841 atacaggcgt cgacaataaa ttgattcagg caaacctcca aaaattgcat gatgctggcg
     9901 cgaatatctg gatacgtatc ccattgatac ctggctggaa cgattttgaa gataatctgg
     9961 ctggacttgt tgaaatgttc aaaaaactgc cgaatatcaa ggctgtggag attatgccat
    10021 atcacaagct tggaataagc aaacttgaac ggtttggttt gaagccgcgc atgataatca
    10081 atagtgaacc gccgagcaaa aaaagcatag accgcttggc aaaattattt tcattctata
    10141 gcggttcttg aaaataaccg tgaatgtttc ggcaacatct gcaaagacaa aaccatcg
//
LOCUS       DAONFI010000037        10196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7467_length_10196_cov_4.554383, whole genome shotgun sequence.
ACCESSION   DAONFI010000037 DAONFI010000000
VERSION     DAONFI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7467_length_10196_cov_4.554383"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLK08_02095"
     CDS             <1..398
                     /locus_tag="PLK08_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016710609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HPS52319.1"
                     /translation="RDNFLNFWFRFIYKNMTAVETENFSYIKDLIERDYDTFSGLMLE
                     SMFREMLAASQNYNEIGSWWDRKGENEIDIVAVNDMKKEILFAEVKLNPKRASLTALE
                     VKAAKLLPEYPGYKTDYVILSTDDIGKYL"
     gene            complement(737..2029)
                     /locus_tag="PLK08_02100"
     CDS             complement(737..2029)
                     /locus_tag="PLK08_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPS52320.1"
                     /translation="MTLSTIYCREDEQTLVARAHAYRILLVVTEPPQEQYLRKYADAM
                     ARSQSAEIGVFRVHELHSGSWTSCHAKFHTEDRGTTAIPELTSHMKLRQRITEIAESW
                     PFDLLIADWPHADPDMGTFAHLVKDIDTTVVLVRHRQCTVRRVLVPAGGGAHALEGIR
                     IADAMAKAWALEKQVLRIVHPGPDFWARRADLKRRCRHIRNATRLYLDVADVEMPVKV
                     RLGGDVADEIICRSRLGDLIVIGGSSQWLMENHASASIPSRVVSGASGPVLMVLTNRG
                     RPSALTDVFWDRTVAVDLTAEDKKHAIAALVDALVDARQVPLGRRDEVVAAAMGRERC
                     GATYIGHGTAIPHAALQGFRGLVGMLGIFPDGVPFGKTEEENASFLFLLLTPKESYEV
                     YLPILARIARFMNEPDNRRQLAQAKTSADVVALLGHAE"
     gene            complement(2863..3675)
                     /locus_tag="PLK08_02105"
     CDS             complement(2863..3675)
                     /locus_tag="PLK08_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPS52321.1"
                     /translation="MKIGIQQTSLGEVGLNRCFSAARLSGITGINLCYNTEEEANDLT
                     SKKHLATINVLQKNFKVAPTGLYMGCLNCFESLIGSAESVAKARKMVTDAITAAHAIK
                     CGTVILPFLGRSKIELTDEMLRACNALSILAETAEDYKINLAVITSLTAEQTEMLLGN
                     IGSDYVKICFDIGVAVACRFDAGCMLRRFGSQKIAQVFLKDVWCNTGMPPEFNVRLGR
                     GDAPLSSIAQSLKAIGYDGWVILDTPPGDKSSTIVNSNVEFTKQLLGQCIPA"
     gene            4369..5064
                     /locus_tag="PLK08_02110"
     CDS             4369..5064
                     /locus_tag="PLK08_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52322.1"
                     /translation="MAVCIGKLKKYANNRKIRFLTALNTTVSNSNEQPTCYIIAGPNG
                     AGKTTFAMDYLPLFVGCRNFINADMIAYGISPLDSLTAQYEAGKLFLNEIHKNIQARK
                     DFAFETTLAGLSHVNLLKTLRQNSWQIILFFLWIPNADFSRDRVRQRAQDGGHDIPED
                     VIYRRYPRIMHNLVHIYTPLCDKIFCYDNSSPEPIPIFSQAKSEIKINDSNIYDQITR
                     CANEYRNIRRNDS"
     gene            5451..6080
                     /locus_tag="PLK08_02115"
     CDS             5451..6080
                     /locus_tag="PLK08_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HPS52323.1"
                     /translation="MLYEGRNVLIEEFGKEYGTYFSILSLIASGKTARPEIESILEHN
                     IGGYLDRLENDFAILSKHRPIGDKPNSKSVKYKIRDNFLNFWFRFIYKNMTAVETENF
                     SYIKDLIARDYDTFSGLMLESMFRELLAASQNYNEIESWWDRKGENEIDIVAVNDIKK
                     EILFAEVKLNPKRASLTALEVKATKLLPEYPGYKADYIILSPDDIGKYL"
     gene            complement(6248..6931)
                     /locus_tag="PLK08_02120"
     CDS             complement(6248..6931)
                     /locus_tag="PLK08_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPS52324.1"
                     /translation="MIQPLIPYAIRGVIWYQGESNNGRSAEYAQLLSTMISAWRERWG
                     QGDFPFGIVQLAGFSTPQEKPDEIDGWTLLCEAQTDVVKSVPNTGLAVAIDIGNASDI
                     HPKNKQDVGYRLAQWALATVYGKKDVEYSGPTFKTMKFDGDKVRISYDHIGGGLVVKG
                     EKPTGFVIAGKNGVFYWADAKIDGDTVVVWSKDVPEPAFVRYGMASNPPVNLYNKANL
                     PAVPFRTDR"
     gene            complement(7063..7593)
                     /locus_tag="PLK08_02125"
     CDS             complement(7063..7593)
                     /locus_tag="PLK08_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52325.1"
                     /translation="MFFEFIFNVNTQHSAIVINICADIYFPERPCRIYGHGRIGKAQP
                     AFKIGKIKQVKHRQFEIHNYPTCVPQSRTEFFLICRPVMKPVFPKVIPKIAFIQGYYQ
                     LAALCPEKCADITIAKSNTLHRIFRRTLIDKVMLRNSARPAYFPFTILYIKRTLHENS
                     LFIFNSKINLPWFHLR"
     gene            7555..9744
                     /locus_tag="PLK08_02130"
     CDS             7555..9744
                     /locus_tag="PLK08_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52326.1"
                     /translation="MLRVDVENKLKEHPATDITLERFGSRTFSHWYSLVNRDATIGLT
                     GTAVSADKTGMYVSHKLTSGTFAVGAKVLADSGAIKYKVEHSFAAATSVLADKFSLVA
                     VVTSPMSGNAISAVKKSMPVDAKDKNISAHKKAWKKFWVRSYMEFGDDYLDNLWHLTM
                     YYANASQRGRYPGRFINGLWTWNRDVQNWNFYFHWNQQEIYWPLNAAGHHDLIESYLD
                     YRFNSLPQAKADAEEILGVDGAIVSDVCDRLGRNSSCEFHNHTPVAQIAMDFWRQYLY
                     TGDADFLAQKVLPYIIEAAKFFESRFEKCGDGKYHTKEATGYEGWILLNDCVTELACA
                     RVLFAVAIEAIRLAGVCEPRAEKWCDILHNLADLPQIDIPADMTTECENDKRYAVGSF
                     VGESIIADKILSAGFGVKERRMLCSWIRRKPDAFKMDNLNVMFRKLEAGITPTVPVDC
                     DLENYAGIFPWCELSAVYPSGLVGLSNADSEIFKTLVTTAKLYSNNSMGWDATPIVMA
                     RLGLGRELAGLLAKMPDRWQFFCNGWGHYGPEMDMKSESAMRFARRNVRDASDVAKES
                     AEPSQYPAPLWPYRHMGMEAMSVLATAMNEALLQSYDGVIRVAPAITAKQNARFTLHA
                     TGGFVVSAEIENGKILWLHVQNKFGGTLKLANPWKRIYCYIGKSNATESTDNLLVLPM
                     QPGQKALFTPDKIGPSDWKLQLLKLLSNKAYKTNETGLSSLGLPRMF"
     gene            9785..9934
                     /locus_tag="PLK08_02135"
     CDS             9785..9934
                     /locus_tag="PLK08_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52327.1"
                     /translation="MITCRNYAWIECSVQELRKRPDSNARSSDGEILNDPAIRDMLEK
                     EFGVI"
     gene            complement(9989..>10196)
                     /locus_tag="PLK08_02140"
     CDS             complement(9989..>10196)
                     /locus_tag="PLK08_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HPS52328.1"
                     /translation="IEPKIGHLKSDHRMNRNYLKHAAGDEINAILAACGANMRKLLRK
                     LSHALMELWLKLQILLQSPAQAAA"
BASE COUNT         2582 a         2493 c         2462 g         2659 t
ORIGIN      
        1 ttcgtgataa ttttctcaac ttctggtttc gtttcatcta caagaacatg actgctgttg
       61 aaacagaaaa cttttcttac atcaaggatc tcattgaacg tgattatgac acattcagcg
      121 gcctaatgct ggagtctatg ttccgtgaga tgttggcggc aagccagaac tataatgaaa
      181 tcggatcatg gtgggaccgc aaaggtgaaa acgaaatcga tattgttgcc gtcaatgata
      241 tgaaaaaaga aatactgttc gcggaagtca agctcaaccc caaacgcgct agtttgacag
      301 cccttgaagt caaagctgcc aaactattgc cggaatatcc tggctataaa accgattacg
      361 taattctcag cacggatgat atcggtaaat acctttgaac tccaacaaac cacaataaca
      421 accaacgcgt ggtggtaaac taactgcaaa ccatcttttg ccgtttgctt tttgcccaat
      481 attcatgggt tttcagcatt gttctgccgc caagctgatc gctgaggcga accccatgtc
      541 gtcctcgttc tcgtagacga agaccgcaag attgccgagg gcaagccgcg caaaaggaat
      601 gggcgcggta cagcgaaatg ctgacgtctc agcattactg tctacggtat gaaggtcgct
      661 cgattccatg ttgatctcac tttctcttgg atggctggtt gttctgatgc cgtcagtctc
      721 tttcgctgga ccactcttat tctgcgtgcc ccaagagtgc gacgacatcg gccgaggtct
      781 tggcctgcgc cagttgccgg cgattgtcgg gctcgttcat aaacctggct atccgcgcga
      841 gaatcggcaa gtatacctcg tagctctcct tgggcgtaag caggaggaac aagaagctcg
      901 cgttttcctc ctccgtttta ccgaagggca ctccgtcggg gaatatgccc agcatcccta
      961 ccaagccgcg aaagccttgc aaggccgcat ggggaatggc ggtgccgtgg ccgatatacg
     1021 tcgcgccgca ccgttcccgc cccatcgcag cggcgactac ctcgtcgcgc ctgcctagag
     1081 gcacttggcg ggcatccacc agcgcgtcca cgagcgccgc aatggcatgc ttcttgtcct
     1141 ctgcggtcaa atccacggcg acggtgcggt cccagaacac gtcggtcaag gccgaaggtc
     1201 gtccgcgatt ggtcagtacc atcaagactg gcccagaggc accgctcacg acgcggctgg
     1261 ggattgaagc cgatgcgtgg ttttccatga gccactggct ggacccgcca atcacaatca
     1321 ggtcgcctag tcgggagcgg cagatgatct cgtcggcaac gtccccgccc agacgcacct
     1381 tcacgggcat ctcgacatcg gcaacgtcca ggtatagacg cgtggcgttg cggatgtgcc
     1441 tgcaccgacg tttaaggtca gcccggcgcg cccaaaaatc cgggccgggg tggacgatcc
     1501 gcagcacctg cttttccaga gcccacgcct tggccatggc gtctgcgatt cggatgccct
     1561 ccagggcatg ggctccgccg cctgccggaa caagcactcg gcgcaccgtg cactggcgat
     1621 gtcggaccag aacgacagtc gtgtcgatgt ccttgacgag atgggcaaac gtgcccatat
     1681 ccgggtccgc gtgtggccaa tcagcgatga gcaggtcgaa cggccagctt tcggcgatct
     1741 ctgtgatgcg ctgccgcaat ttcatgtgcg atgtcagttc gggaatcgcc gtcgtgccgc
     1801 gatcctcggt atggaatttg gcgtgacagg acgtccacga accgctgtgc agctcatgca
     1861 cgcgaaacac gccgatctcc gcagattggg accgcgccat cgcgtcggcg tacttacgca
     1921 ggtactgttc ctgcggcggc tccgttacaa ccagcaagat gcgataggcg tgcgcccgcg
     1981 ccacaagagt ctgctcgtct tcccggcagt agatggttga aagggtcatg acttcgtctc
     2041 ctgtgaactt cttctagcgt aatacggttt cgtgcgcctc gacgacgatc atcctaggcg
     2101 acgacggcac gtccaggctc gccacgtatc cggtcaccgc ctcgcgcatc ttgtgctcgg
     2161 ctgactcccg tgtgcatgca ctagccacgc atcccggcag ggacggacag tgcgcgacgt
     2221 attgcccgtc agtcctcgct cgaatcaata ttgcgatttt catgtcagtc tcctttgacg
     2281 gactcagccg tcgaggatcg cgagcaccca gcgcccacgc accccgtcgc tcttccaacg
     2341 caaggtatac atgcatgcct cgcagacgaa ctcatcgcac tctttgtgct tggttcacgg
     2401 tatcctgtgc ctcaccctgg gcagttaccg ggcctattcc cgagacagac atagcctatg
     2461 gttacaactg gccggagatg ccccgagcag aatcgagtaa gagctaaccg cccaccccat
     2521 tataggtaaa tataagcatc aactgttttc aagaatttcc aaggcccagc ggcttttctt
     2581 tccgtcgggt ttccatatca gggcgttcag gcatgcctga caaacgaaag cttcacattc
     2641 gagatgcttg ttcgggacga ttcgcagtcc gcaccctggg cagttgtatg attgctgcat
     2701 ttttttggtt ttgtccgtca aggtcataac atattccttt tttgttaaaa tttttgaatg
     2761 ccgaaaatcg caaaaccgca catgccgcac ttcgaagtct gccctgttgc caagactcgc
     2821 agcaatcggc atgacgtcaa accgctattt cctgaagctt tttcacgctg gaatacactg
     2881 gcctaaaagc tgtttcgtga attcaacgtt tgaattgacg atagtgctgc ttttgtcgcc
     2941 ggggggggtg tcgagaatga cccatccgtc gtagccgatg gctttgagtg attgggcaat
     3001 gctggacaga ggcgcatctc cgcgaccgag acgcacgttg aattcaggcg gcatgccggt
     3061 attgcaccac acatctttga gaaatacctg cgctattttc tgacttccga atcgtctgag
     3121 catgcatccg gcatcgaagc ggcaagcgac ggctacgcct atgtcgaaac atattttgac
     3181 gtaatcgctt ccgatgttgc ccagaagcat ttcggtctgc tcggcagtga gactagtaat
     3241 tacagcgaga tttatcttat aatcctcagc cgtttctgcc aatattgaca acgcgttgca
     3301 ggcccgtaac atttcatccg tcagctctat cttacttctg cccaaaaatg gaagaatgac
     3361 cgtgccgcat ttgattgcat gtgcagccgt aattgcgtcc gtgaccattt tacgggcttt
     3421 tgcgacactt tcagcagagc ctatcagaga ctcaaaacag ttcaggcaac ccatatataa
     3481 acccgtcggg gcgaccttga aatttttctg caggacattt attgtcgcca gatgtttttt
     3541 cgacgtcaag tcgttagcct cttcttccgt gttgtagcaa agattaatgc ccgttatgcc
     3601 gctgagcctg gctgctgaaa agcatctgtt caggcctact tctccgagtg aagtttgctg
     3661 gattccgatt ttcatgatat tactccttat tcaaggataa tttgttctat ccctacagtt
     3721 gcactttgct ttaaaaatgg ccattctagg gcgcctccga agaatctgta ccgcatgttt
     3781 gagatgcttc gcaggcttgg caagatatta caatgaaact catttcataa atggcgttaa
     3841 ctgcaacctc gcctgctgtc ttggaccggg ttgcagaata cgtaatatcc agcaaaaaac
     3901 tcagtttgtg caatgccaca aaagcaggtg tcgctaaatt ggaaattatt taggtcaaca
     3961 caaattgtgt atgagagtac cagcgatttg ccggaaatga gtgaaaaaca agcagtgtga
     4021 atgctatatc atcgtcaaaa accagcaaaa ggcatgcgcc tgctgactga caagatggaa
     4081 ctacgcggtt ttttgtagta acgatgatac actgcaaatc tcctattcaa aagtattgtc
     4141 ccaattttga aactaaaagt caagccttta aatactcaaa ctgagggttt tttgggtaag
     4201 tacagtcatg gcaatgctct cccttcgtaa ctacttgcag ttatagcctc tgcgagtccc
     4261 gtcagcagtc gataatgcaa aaaatacctc gattaccaat ttcctctgct aaatcttaat
     4321 gctttatgtc tttggttacg attgtgtttt tcttgtggga ttgatataat ggcggtttgc
     4381 ataggtaagt taaaaaagta tgcgaacaac agaaaaataa ggtttttaac cgctttgaat
     4441 acaactgttt ccaactcgaa cgaacagccg acatgttata tcatagctgg gcctaacggg
     4501 gctggcaaaa ccacttttgc tatggattat ctgcctcttt ttgtcggctg ccgtaatttc
     4561 ataaacgccg acatgattgc ctacggaatt tctccgcttg actcattgac tgcacaatat
     4621 gaggctggca aattgttctt aaatgaaatc cacaaaaaca tccaagccag aaaagatttt
     4681 gcttttgaaa caactcttgc cggccttagc catgtcaatc tgttaaaaac gctcaggcag
     4741 aattcatggc aaataatctt gttcttttta tggataccga atgctgattt ttcccgtgac
     4801 cgggttcgtc agcgagctca agatggcggc catgatattc cagaagatgt tatctaccgg
     4861 cgttatcctc gaatcatgca taatctggta catatctaca cgccgttatg cgataaaatc
     4921 ttttgctacg ataactcttc acctgaacct ataccgatat tttcccaagc taagagtgaa
     4981 atcaaaataa acgattcaaa catatatgac caaataacaa ggtgtgccaa tgaataccga
     5041 aacattagaa gaaatgacag ttagcgcagg cgaatgctta aagcaggccg tacggaaaga
     5101 actcaacaca aaggctaaac taggccaata tgcgataatt aatcgtaata acaaaccgtg
     5161 ccgagtgcct gcctctgaag cgttgagaat cgctgaaggc aagcaggctt gactgtctgt
     5221 agtgcatcac ggcacagtag gctaactgta aaccgaaaat aatggtttac agttagccga
     5281 tgttcacagg catcaattcc tcagttttcc acttgctgca ctcaaagttg ttggcttgtg
     5341 ttttataaaa ctcaatttct ttcacccacc ttccaagctg ttgggagttc aagtctcttc
     5401 ggaaaacact ttgaactcca gttgatttca tactcaatac cagctctcca ttgttatacg
     5461 aaggcaggaa tgttctgata gaagaattcg gcaaagagta tggaacatat ttttccatac
     5521 tctcgcttat tgcttcgggc aaaaccgcaa ggccagaaat agagtcaatt ttggaacaca
     5581 atatcggcgg ctatcttgac agacttgaga atgacttcgc tatactttca aagcatcgcc
     5641 ctattggcga caaaccaaac tcaaagagcg tcaagtataa gattcgtgat aattttctca
     5701 acttctggtt tcgtttcatc tacaagaaca tgactgccgt tgaaacagaa aacttctctt
     5761 acatcaaaga tctcattgcg cgcgactatg acactttcag cggccttatg ctggagtcaa
     5821 tgttccgtga gttgctagcg gcaagccaga actataatga aatcgaatca tggtgggacc
     5881 gtaaaggcga aaatgaaatc gatattgttg ctgtcaatga cataaaaaag gaaatcctgt
     5941 ttgcagaagt caagctcaac cccaaacgcg ctagtttgac agcccttgaa gtaaaagcta
     6001 ccaaactatt gccggagtat cctgggtata aagccgatta cataattctc agcccggatg
     6061 atatcggtaa atacctttga actccagcaa gtcataatat caatcaacgc gtggtggtaa
     6121 actaacagca aaccatcttt tgccgtttgc tttttgccca acattatagg ttttcaaaca
     6181 ctcgatgctc agcttgctac gccggcagtc gtgggttcag agtaccgcaa gatgaaaaat
     6241 attttgtcta cctgtctgtg cgaaatggta ctgccggcag gtttgcctta ttatacaggt
     6301 tcaccggcgg attactggcc atcccgtaac gcacgaatgc gggttcgggt acatctttcg
     6361 accacacaac cacggtgtcg ccgtcgatct tcgcatccgc ccaatagaac actccgttct
     6421 ttccggcgat aacgaaaccg gtcggttttt cgcctttcac aaccaagccg ccgccgatat
     6481 ggtcatatga gatacgcacc ttatcgccat cgaacttcat cgtcttgaac gtcggcccgg
     6541 aatattcgac atctttcttt ccataaaccg tcgccaacgc ccattgcgcc agacgatacc
     6601 ctacgtcctg cttgtttttc gggtgaatat cggacgcatt gccgatgtca atcgctaccg
     6661 ccagtcccgt attcggaaca ctcttgacga catccgtctg ggcttcgcac aacaacgtcc
     6721 atccgtcgat ttcatcgggc ttctcctgcg gggtactgaa tcccgccagc tgaacgatac
     6781 cgaatggaaa atcgccttgt ccccatcgtt cccgccatgc ggaaatcatt gttgaaagca
     6841 gttgtgcata ttccgcagac ctgccgttat ttgattctcc ctgataccag atgacaccgc
     6901 gaattgcata tggaattagc ggctgaatca ttccgcgatg cggcggaaaa tgtagaattg
     6961 atgctccaca catccggcga taagataaag tttgtggaca tggaaaatat tgagatcaaa
     7021 gttaccgcaa aatgtcgcga cggtgaatac gcctgtttcg tgttaccgca aatggaacca
     7081 tggcaggttt atcttgctgt tgaaaatgaa taaggaattt tcatgcaaag tgcgtttgat
     7141 ataaagaatg gtgaatggaa aataagctgg cctggcagag ttgcgaagca tgaccttgtc
     7201 tatcagagtc cgccggaaga tccgatgcag ggtattgctc ttggcaatgg tgatgtcggc
     7261 gcacttttct ggacacaggg cagcaagttg ataatagcct tgaataaatg cgatctttgg
     7321 gatgaccttg ggaaaaacag gtttcataac tgggcggcag atgaggaaga attcagtccg
     7381 actctgcggc acgcatgtcg gataattgtg gatttcaaat tgccggtgtt tgacctgttt
     7441 tatctttccg attttgaagg ccggctgagc cttgccgatg cgaccatgtc catatatgcg
     7501 gcagggcctt tcgggaaaat aaatatccgc gcatatgttg atcacaatag cggaatgctg
     7561 cgtgttgacg ttgaaaataa actcaaagaa cacccggcaa ctgatattac tcttgagcgg
     7621 ttcggctcgc gcacattttc acactggtac agtcttgtaa atcgtgatgc tactatcggc
     7681 ctgaccggaa cagcagtatc ggctgacaaa accggcatgt atgtttcaca taagctaacg
     7741 agcggaacgt tcgccgttgg cgcaaaagtg cttgccgatt ctggtgcaat caaatacaaa
     7801 gttgaacatt catttgcagc cgcaacttca gttttggctg acaagttttc gctggttgct
     7861 gttgttactt caccgatgtc gggcaatgcg atatctgctg ttaaaaaatc catgcccgta
     7921 gatgctaaag acaaaaacat atccgctcac aaaaaagcgt ggaagaaatt ctgggttcgg
     7981 tcgtacatgg aattcggtga tgattatctg gacaatctct ggcatttgac gatgtattat
     8041 gcaaacgcgt cgcagcgagg aagatatcca gggcgtttca tcaacggtct gtggacgtgg
     8101 aatcgcgatg tgcagaactg gaatttctac tttcactgga atcagcagga aatatattgg
     8161 cctttgaatg cagcagggca tcacgacctg attgagtcgt atcttgatta taggttcaat
     8221 tctctcccgc aggccaaggc ggatgcagaa gaaattcttg gagttgacgg cgcaattgtc
     8281 tctgacgtgt gcgacaggct aggccgcaac agctcatgcg aatttcacaa tcatacaccg
     8341 gttgcccaga tagccatgga tttctggcgg cagtatttgt atacgggcga cgccgatttt
     8401 cttgcgcaga aggtattgcc gtatataatt gaagcggcca agttttttga gtcgcgtttt
     8461 gaaaaatgcg gtgatggaaa atatcacacc aaagaggcca caggttatga aggctggata
     8521 ttgctcaatg attgcgtgac tgagcttgcg tgtgcacggg ttctttttgc ggtagcgatt
     8581 gaggccatcc gtcttgctgg cgtatgtgaa cctcgtgcgg aaaagtggtg tgacattctg
     8641 cacaatttag ccgacttgcc gcaaatcgat attcctgccg atatgacgac agaatgtgaa
     8701 aacgataaac gttatgcagt tggttcattt gtcggcgaaa gtatcattgc tgataaaatt
     8761 ctctctgccg gatttggagt aaaagagaga cgcatgctgt gctcgtggat tcgccgtaag
     8821 cctgacgcat tcaagatgga caatctcaac gtaatgttcc gaaaactcga agcaggcatt
     8881 acgccgaccg ttccagttga ttgcgattta gaaaattacg ctggaatttt tccgtggtgt
     8941 gaattgtcag cagtttaccc gtctggcctt gttggcttgt caaatgcgga ttctgaaatt
     9001 tttaaaacgt tggttactac tgccaagctg tattccaaca attcaatggg ttgggacgca
     9061 acgcctattg tcatggcgag gcttggattg ggtcgggagc ttgcagggct gctcgcaaaa
     9121 atgcctgaca gatggcagtt cttctgcaac gggtggggac attatggccc ggaaatggat
     9181 atgaagtcgg aaagtgccat gcgttttgcg aggcgtaatg ttcgagatgc ctcagatgtc
     9241 gcaaaagaga gcgcagaacc atctcaatat cctgcacccc tgtggcctta tcgccacatg
     9301 ggtatggagg cgatgtctgt ccttgcgacg gcgatgaatg aagctttgct tcaaagttat
     9361 gacggggtca ttcgtgttgc tcctgcaatt actgcaaaac agaatgcaag attcacgctg
     9421 catgccaccg gcggattcgt agtctctgct gaaattgaaa acgggaaaat tctgtggctt
     9481 cacgttcaaa ataagtttgg cggaacgtta aagttggcca atccgtggaa acggatatat
     9541 tgttacattg ggaaaagtaa tgcgacagaa tctaccgata atctcttggt tctcccaatg
     9601 caaccagggc agaaagcatt gtttactcct gacaaaattg ggccgtctga ctggaaactg
     9661 caattactta agttactttc caataaagcc tacaagacga atgaaacagg gttgagttct
     9721 cttgggctgc cgcgaatgtt ctgatcatgg cgttactgtt ccagaaagcc agttgcggca
     9781 tctcttgata acttgccgaa attacgcctg gatagaatgt tcggtacagg aattgcgtaa
     9841 acgtcccgat tcaaacgcaa gaagttctga cggtgagatt ctcaacgacc cggctattcg
     9901 cgacatgctc gaaaaggaat tcggcgtgat ataatgagtc gtccctgaaa aagtcggatt
     9961 ttgactttat cgattatttt tcatctgttc aggcggcggc ttgggctggc gattgcagca
    10021 ggatttgcag tttcagccat aattccatca gcgcatggct gagtttcctc agcaatttgc
    10081 gcatattcgc accgcaggct gcgagaatcg cgtttatctc gtcgccggct gcatgtttga
    10141 gatagttcct gttcattcga tgatcgctct tgaggtggcc tatcttcggc tcaatc
//
LOCUS       DAONFI010000038        10088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7590_length_10088_cov_4.146018, whole genome shotgun sequence.
ACCESSION   DAONFI010000038 DAONFI010000000
VERSION     DAONFI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7590_length_10088_cov_4.146018"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLK08_02145"
     CDS             complement(<1..598)
                     /locus_tag="PLK08_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPS52329.1"
                     /translation="MTNKSIVVAGHICVDMIPDMSACKGPLDKVIVPGKLVNVGAPLM
                     STGGVVANTGLALHKLGLPVKLLGKVGDDSFGQIICQIVGANSPQLSRDMIISRGEST
                     SYSVVISVPGCDRAFLHYPGTNNTFCADDINYESLEGVSLFHFGYPPLMRKMFLNNGA
                     ELAQMFGKVKAMGITTSMDMTLPDPDSESGRVDWACIRA"
     gene            1014..1901
                     /locus_tag="PLK08_02150"
     CDS             1014..1901
                     /locus_tag="PLK08_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPS52330.1"
                     /translation="MSKDKNSLKDLLSPVAQSVKKDLDMWLIEAGTPSSLAAAMRYCM
                     EGGKRLRPALVMFSAQAAGGNAEDELVRRCAVAVEMVHVYSLVHDDLPAMDDDELRRG
                     RPTAHIKFGEAMAILTGDSLLTRAFGVLAQSGSPLAGRLAGELSAAAGPTGMVGGQVA
                     DMAMCDVPAGYDGLEYIHRRKTGAIITAAARMGAIAAGADENKLAAISDYASALGLAF
                     QVCDDILDATGSTVELGKTAGKDLQAGKRTVVSELGLEKACELNESLTAQARAALEPL
                     GHKADILAELVGLLAERTN"
     gene            1947..3824
                     /gene="dxs"
                     /locus_tag="PLK08_02155"
     CDS             1947..3824
                     /gene="dxs"
                     /locus_tag="PLK08_02155"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPS52331.1"
                     /translation="MDKKDTLLSRINSPADIRKLSLEELERLADEVRDRVIEVVSRNG
                     GHLASNLGVADLTIALHYVFDFPTDRLLWDVGHQCYAHKLLTGRAGRFDTLRQGGGLS
                     GFPSPAESDYDLFYTGHAGTAISTAAGLAWADKMQGRNDRKCVAVVGDASIVNGMSLE
                     GLNNAALLKRQFLVILNDNSMAIDRTCGALAVALEKIRMTPKYADLKSSTEMLLKKLP
                     LGGEITGALKHIREGLRTTLHGPRVFETLGFNYFGPISGHDIGTLIRVLQRMKELNRP
                     VVLHVHTEKGRGCQYAVDDPCRFHSPSAHKISNGKAVFKKLDRPIWTQCFADILIEKA
                     AADPRIVAITAAMPDGTGLAKFREKFHDRMIDVGISEEHAIATAAGMAKSGLIPVVAI
                     YSTFMQRAFDQVFHELALQGLHVILCMDRAGLVGSDGAVHHGLTDVSFMRTLPGMTLI
                     APATEAELNAALDYAIKCTGPVAMRYPRDLIPAGSPSTCPPFVTGKAREMRQGKDVSL
                     ICYGAIVNEALAAADMLAEVGIESQVINARFVKPLDEELLCAVIATGRRILTIEDHFV
                     AGGFGSAVLEMAAGKGLDASKITLLGVGEEYIQHDTRAAQLIAAGLDSHGIAEKVKTL
                     L"
     gene            complement(3845..4285)
                     /locus_tag="PLK08_02160"
     CDS             complement(3845..4285)
                     /locus_tag="PLK08_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52332.1"
                     /translation="MKEVRMEHTEGYGQPAEVWVDGTLYTVCDNISSPGQRTRPGLIE
                     GAEFQYHNEEGYSWDDAVAGNPSHRIVIEPVKGWGYVGFGRVEQVMPVVINFGQLRME
                     DPNWSTDERLVGQFVRVPINRLEIVPRVRPDWPENFKVNSRSEK"
     gene            complement(4292..5497)
                     /locus_tag="PLK08_02165"
     CDS             complement(4292..5497)
                     /locus_tag="PLK08_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS52333.1"
                     /translation="MEVMISDRLAMLPPYLFADLRRKIAAKRAQGIRVITLGIGDPDM
                     PTPDPIVQEMIRAINDSQDANRHRYGCDVPVAAFPEACRNFYKHRWGVELGPDQVITT
                     MGSKDAIAKFPMAVMNPGDIGIAPVPGYPTYNIGHVFAGGSTYYVPLRAERKFLLDFS
                     EIPTDIAKKAKLLWINYPNNPTTATAPLSFFEEAVAFGRQYDILICHDSAYSENTYDG
                     YSAPSILQVKDAADVAVEFFSLSKAFCMTGWRAGCVSGNASAIKALSLTKDNVDNGTL
                     RAVQFAAAKALSLRDQLVPKINEVYKRRRDMVLTLLNENGWHMEKPVSTIYLWVPVPE
                     KWRKNDGQPGYSSSGKFAEELLEKYGVVITPGRGYGQTGEGFFRVSLTYPDDVIKEAV
                     EKIIKLTKE"
     gene            5710..6618
                     /locus_tag="PLK08_02170"
     CDS             5710..6618
                     /locus_tag="PLK08_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS52334.1"
                     /translation="MRMHKKLLDILGILAACTAAWTAGCDETLQSAGSQSPAASVQAK
                     AAQTNVGGPMPFRDASALADRQAAEITGDTPEDIQMRQQFRQAVIDLLSSPDAETRRE
                     SAEMLGVLHKKHALSALTLAVDDPDAKVQEAAILAIACIGEKTVAGQIATKLNSPQIR
                     VRAAAAKTLGRLATPKTTGALISALKDSSADVRESAARALGPLHEQESIEPLIRALRD
                     ADPKVRAAAAGSLGIMQAPQAVPPLIKTLKDPYAQVRYLAAGALGNLGDKSALPALAS
                     LRNDSDIAVRNMAEAAISKLRGPKKP"
     gene            complement(6657..7919)
                     /gene="murA"
                     /locus_tag="PLK08_02175"
     CDS             complement(6657..7919)
                     /gene="murA"
                     /locus_tag="PLK08_02175"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPS52335.1"
                     /translation="MDKFIIEGGTELSGNIEVGGAKNSALPMLAATMLCDGRSVLHDV
                     PDLADIHAMVDLMAELGVSVERSGHSLQIEVTDESLSHAPYDRVRKMRASICVLGPLL
                     AKRRSARVSMPGGCAIGTRPVDLHLRGMRELGAEIHLEGGDIIASTARLRGAEIFLGG
                     AFGSTVLGTLNVMMAATLARGTTLIECAACEPEVADVADFLNKAGAKISGAGTPRIII
                     SGVDSLNGVEHRIIPDRIEAGTFMLAGALCGRDVCVKNVRIDHLLALVDKLRNIGVAL
                     ENSDGTVKVSRPDRIRPADVTTQPYPGFPTDLQAQLMVLLCLADGNSAITEKIFPDRF
                     MHVPELMRMGADIRKEGPTAIVTGVPELIAAPVMASDLRASAALVMAGMVARGRSEVR
                     RVYHIDRGYEKIETKLASIGAKITRGQE"
     gene            complement(7973..8986)
                     /locus_tag="PLK08_02180"
     CDS             complement(7973..8986)
                     /locus_tag="PLK08_02180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HPS52336.1"
                     /translation="MNESWTTGRLLSWTKEYFEHSGLENPRLCAEVLLSHCVGCSRIE
                     LYARFNSEPTDRQRSQFRELVRRAAMHEPVAYLVGSREFYSLNFRVTPAVLIPRPETE
                     MLVEKAVEHLQKLAGVHVTQQKFDSQESSPVDLDEIEKLASAQPEESQSSEPAFQPKK
                     NRSQYVGNVWDVCTGSGCVACAIAANAPGVNVLASDISPAAIAVAVENASRLNLSQNV
                     TFEQADLLELPVGSRYNDEYFDVITANPPYVAENDPVGDSTKYEPRDALYAGIDGLDV
                     LRRLIPAVPKRLGRGGIFCVEFGYTHADAVRNLIVQTGEFAEPEIFKDFQGIERVAVA
                     KRV"
     gene            complement(8983..>10088)
                     /locus_tag="PLK08_02185"
     CDS             complement(8983..>10088)
                     /locus_tag="PLK08_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52337.1"
                     /translation="APYFAVGFFRALILLGIDSAFSITESTLASIVDKTGWSKNWTLV
                     ILSLLGLGVGLIYCCQGGLNWLGVFDDYINGTWGITLTALAEGLVLGWLIRLRRLREH
                     ANERSDWRLGVWWDFSMRIIIPVVMAALFAWNLLDEFGNPYYLRDAGGGLNIGNIAGL
                     AVMAAAPVLAVVITSINFKKTHDEPIRPEYLIKTPHGRVYGIVAVMLSLDGLFFLALA
                     FLRLISILHIQKYHMGDAGAVAAKAALAGVLLLKGGSFCLIATVIAGVGMVWYESRKI
                     LASMVLRIGGALSIMLLGCTAGLGMALWAMASDVKIRPLEYNGQLDGDSYLILFLMAG
                     IIICGLGWCFYRALRAAGQSDTEEQSAEIVRNT"
BASE COUNT         2374 a         2953 c         2644 g         2117 t
ORIGIN      
        1 tcgccctgat gcaggcccag tccacacggc cggattcgct atccgggtcc ggcagggtca
       61 tgtccatcga cgtggttatg cccatcgcct tgactttgcc gaacatttgg gccagttcag
      121 cgccgttgtt aaggaacatt ttccgcataa gcggcggata accgaaatgg aacagcgata
      181 ccccctccag cgattcatag tttatatcgt cagcgcagaa ggtgttgttc gtgcccgggt
      241 aatgcaaaaa ggccctgtcg cagccgggca cggaaatgac gacggaatac gacgtcgatt
      301 cgccgcgaga gataatcatg tcgcgtgaaa gctgcggcga attcgcgccg actatctggc
      361 agataatctg gccgaatgaa tcgtcgccga ccttgccgag cagtttgacc ggcaggccga
      421 gcttgtgcag cgcaaggcct gtattagcca ccacgccgcc ggtggacatc agcggcgcac
      481 cgacatttac aagcttgcca ggaactatca ctttatccag cgggccctta catgccgaca
      541 tgtcgggtat catgtcgacg cagatgtggc cggcgacgac gatgcttttg ttcgtcatac
      601 tgtctcctca tatcagtgtt tatcgtacta aaacatgctg cttcagggac accgatggcc
      661 acaattccat attgtcggcc agtcaaaaat acaggtgccc gtatgatatc tttgccgaca
      721 gcctcccgtc aacatgagaa tgacaggaaa aaacggcaca ggcctttttt ttgacgccag
      781 aaaaggtata atgcccacaa atgttgtgaa ttgcactgcc gccggcggca gaaacaataa
      841 aaaacagcaa aacttcaatt ttcggcaatt tcaaaaagac cggctcagga gttttgggcc
      901 gtaattgtct gccctaaaag ttttgcccga tttttgcagg atttttctgt cgttgtttat
      961 aattgataac tgccggctat aatcggactt gtgatatact tggatgcaaa aaaatgtcaa
     1021 aagacaaaaa ttcgctcaaa gatttattgt ctccggtagc ccagagcgtt aaaaaagacc
     1081 tcgatatgtg gcttatcgaa gctggcacgc cctcctcgct agctgccgcc atgcgttatt
     1141 gcatggaggg cggcaaacgg ttgcgtcctg cgctggtgat gttttccgca caagccgccg
     1201 gcgggaacgc ggaagacgaa ctcgtccgcc gctgcgcagt agccgtcgaa atggtacacg
     1261 tttacagcct cgttcacgac gacctgccgg caatggacga cgacgaactt cgtcgcggcc
     1321 ggccaaccgc acacatcaaa ttcggcgaag cgatggctat tttgactggc gattcgcttc
     1381 tgacgcgtgc gttcggcgta ctggcgcaga gcggttcgcc gctggcggga aggctggcag
     1441 gcgagctttc agcggcggca gggccgacgg gcatggtagg cggccaggtg gcggacatgg
     1501 cgatgtgcga cgtgccggcg ggctatgacg gcctggaata catccatcgc cgcaaaaccg
     1561 gcgcaatcat aaccgcagcc gcccgtatgg gtgcaatcgc agcaggcgcg gacgaaaata
     1621 aactcgcagc catctcggat tacgccagcg ccctgggcct tgcgtttcag gtctgcgacg
     1681 acatcctgga tgccaccggc agtacggtcg aactcggcaa gacggcaggc aaggatctgc
     1741 aagccggcaa acgcaccgta gtctccgaac tcggactgga aaaagcctgt gaactgaacg
     1801 aatcgcttac cgcccaggcc agagccgctc tcgaaccgct cggccacaaa gccgacattc
     1861 tcgccgagct tgtcggcctg ctggcggaaa gaacaaattg acacggctgt ttaactctgt
     1921 aaaaattatc gaaccggtat tggaaattgg ataaaaaaga cacattgctt tcacgcataa
     1981 attctccagc cgacattcgc aagctgtcgc tggaagagct tgagcggctg gctgacgaag
     2041 ttcgcgaccg cgttatcgag gtcgtcagcc gcaacggcgg ccatctcgcg agcaatctcg
     2101 gcgtagccga ccttacgata gccctgcact acgttttcga ttttcccact gaccggctgt
     2161 tgtgggacgt cggccatcaa tgttacgccc acaaacttct caccggccga gccggccgat
     2221 tcgacacatt gcgccagggc ggcgggctga gcggttttcc cagtccggcg gagagcgatt
     2281 acgacctttt ttataccggc cacgccggca cagccatctc gacagccgcc ggccttgcat
     2341 gggcggacaa gatgcagggc cgcaacgaca ggaaatgcgt agccgtcgtc ggcgatgcga
     2401 gcatagtcaa cggcatgagc ctggaaggcc tcaacaacgc cgcactgctc aaacgacagt
     2461 ttctggtcat actcaacgac aactccatgg ccatcgaccg cacatgcggc gcgctggctg
     2521 tcgcgctgga aaaaatccga atgaccccaa aatacgccga cctgaaatca tccaccgaga
     2581 tgctgctcaa aaaactgccg ctcggcggag agattaccgg cgcactcaag cacattcgcg
     2641 agggccttcg caccacgctg cacggcccgc gggttttcga aacgctggga ttcaattatt
     2701 tcgggccaat aagcggacat gacatcggta cgctcatccg cgtactgcaa cggatgaagg
     2761 agcttaaccg gccggtcgtt ctgcacgtac ataccgaaaa aggccgcggc tgccagtacg
     2821 ccgtagacga cccatgccgt ttccacagcc cgtcggcaca caaaatatca aacggcaaag
     2881 ccgtcttcaa aaaactcgac cggcctatct ggacgcagtg cttcgcggac attttaattg
     2941 aaaaagccgc cgccgacccc cgcatagtgg ccatcaccgc cgcaatgccc gacggcacag
     3001 gcctggcgaa attccgtgaa aaattccacg acagaatgat agacgtcggc atcagcgaag
     3061 aacacgctat cgcgacggca gccggcatgg cgaaatcggg acttataccc gtagtcgcca
     3121 tctattccac attcatgcag cgcgcgttcg accaggtttt tcacgaactc gcacttcagg
     3181 gcctgcacgt gattctatgc atggacaggg ccggtctcgt gggcagcgac ggcgcagtgc
     3241 atcacggcct gaccgacgta agtttcatgc gcactctgcc gggcatgacg ttaatcgcgc
     3301 ctgcgaccga agccgaactc aacgccgctc tggactatgc gattaaatgc accggccccg
     3361 tggcgatgcg atatccgcga gacctcatac ccgccggttc gccgtcgaca tgtccgccgt
     3421 tcgtcacagg caaagcccgc gaaatgcggc agggaaagga cgtgtcgctg atatgctacg
     3481 gcgcgatagt caacgaagct cttgccgcgg cggatatgct cgcggaggtc ggcatagaat
     3541 cgcaggtcat aaacgcgcgt ttcgtaaaac cgctcgatga agaattgctg tgcgcggtta
     3601 tcgcaaccgg ccgacgaata ctgacaatcg aagaccattt cgtcgcaggc ggcttcggca
     3661 gtgcagtgct cgaaatggcg gcaggaaaag gactcgacgc ttcaaaaata actctgctgg
     3721 gcgtgggcga agaatatata caacacgata ccagagcagc acaactcatc gcagccggcc
     3781 tggattcgca cggcatcgct gaaaaagtca aaacacttct ataacgttca gcgaaaatcc
     3841 gacttcactt ttcagaccgg gaattgactt tgaaattttc aggccagtcg ggacgtacgc
     3901 ggggcactat ttccagccgg ttaatcggca ctcgcacgaa ttgacccaca aggcgctcgt
     3961 cggtactcca gttggggtct tccattcgca gttggccgaa atttattacc acgggcatga
     4021 cctgttcaac tcggccgaaa cccacgtatc cccagccttt gaccggctcg ataacgatgc
     4081 gatgactcgg attgccggcg acagcgtcat cccaggaata gccttcttcg ttgtggtact
     4141 gaaactccgc gccttcaatc aggcccggcc tggtacgttg gcccggcgag ctgatattgt
     4201 cgcagacggt atacagcgtg ccgtccaccc aaacctcagc cggctggcca taaccttcgg
     4261 tatgttccat tcgcacttct ttcatgcgtg gttattcttt cgtaagtttg atgatttttt
     4321 cgactgcttc cttgattacg tcgtcggggt aggtcaggct gacgcggaaa aatccttcgc
     4381 cggtctggcc gtagccgcgg ccaggcgtta tgactacgcc atatttttcc aaaagttctt
     4441 cggcaaattt tccggatgaa ctgtaaccag gctggccgtc gttctttcgc catttttcag
     4501 gcaccggcac ccagagataa atcgtgctca ccggcttttc catatgccag ccgttttcat
     4561 tcagcagcgt cagcaccata tctcggcggc gtttgtatac ttcgtttatt ttaggcacta
     4621 actggtcgcg gagactgagc gcctttgcgg cggcaaactg taccgcgcga agcgtgccgt
     4681 tatcgacgtt atccttcgtc agcgagagag cttttatcgc agacgcattg ccgctcacac
     4741 agccggctcg ccagccggtc atgcagaacg cctttgaaag gctgaaaaat tcaacggcaa
     4801 cgtcggcagc atccttaacc tgcaatatgc tcggcgcgga atagccgtcg taggtatttt
     4861 cgctatacgc cgaatcatgg cagatgagta tgtcgtattg acggccaaac gcgaccgcct
     4921 cttcgaaaaa actcagcggc gcagtggcag tagtaggatt gtttggataa ttaatccaca
     4981 acagcttggc tttttttgca atatccgtgg gtatttcaga aaaatccagc aaaaatttcc
     5041 gttccgcccg caacggcaca taatacgtgc tgccgccggc gaaaacatgg ccgatattat
     5101 acgtcggata acccggcacc ggagcaatgc cgatgtcgcc aggattcatc acagccatgg
     5161 ggaacttggc gatggcgtct ttcgacccca tagtggttat gacctggtcg gggcccaact
     5221 ccactcccca gcgatgctta tagaaattgc ggcacgcctc cggaaaagcg gctaccggca
     5281 catcacagcc gtagcggtga cggttggcgt cctgagaatc gttgattgcc cgaatcattt
     5341 cctgcactat cgggtccggc gtgggcatgt ccggatcgcc gatgccgagg gttatcaccc
     5401 ttatgccctg agcgcgtttt gcagcgattt tacggcgtaa atcggcaaaa agatacggcg
     5461 gcagcatcgc cagccggtct gaaatcatga cttccatttc caaatccttt ctaaaacggc
     5521 tgatttttgt gctttcaggg tttataaaaa cctctgaaaa tggccagccg gtttgaaatt
     5581 tgtcctattt tttacaaaaa agttctacaa cttgacccaa aagactgaaa accaaatttt
     5641 gcgacagacg caaaaagtgc attttagcga ttccggctct tgaggcaatg ccttaaacgt
     5701 gatatatcta tgcgaatgca taaaaaattg ttggacatac ttggcatatt ggcagcctgt
     5761 actgcggcat ggacggctgg ttgcgacgag acattgcagt cagccgggtc acaatcgcca
     5821 gccgcatccg tgcaggctaa agccgcgcag acaaacgtcg gcgggccgat gccatttcgc
     5881 gacgccagtg cgcttgccga ccgtcaagcc gcagaaataa ccggcgacac acccgaagat
     5941 atacagatgc gacagcaatt caggcaggcc gtcatcgacc tgctgagcag tccggatgca
     6001 gaaacgcgtc gcgagtcggc tgagatgttg ggcgtattgc ataaaaaaca cgcgttaagt
     6061 gcgcttacgc tggcagtgga cgaccctgac gcgaaagtgc aggaggctgc gatattggcg
     6121 atagcatgca tcggcgaaaa gaccgtcgcc ggccagattg ccacgaaact gaactcgccg
     6181 caaatccgcg tacgtgccgc agcggcaaaa acgctcggcc gtctggcaac gcccaaaacc
     6241 accggcgcac tcatatccgc cctgaaggat tcatccgccg acgtacgcga atccgccgca
     6301 cgcgcgcttg gcccgctgca tgagcaggaa agcatcgagc cgcttatacg tgctttgcgg
     6361 gacgccgacc ccaaagttcg agccgcagcc gccggttcac tcggaatcat gcaggctccg
     6421 caggctgtac cgcccctgat taaaacactc aaagacccct acgcccaggt acgatatctt
     6481 gccgccggcg cacttggcaa tctcggcgat aagtctgcac tgccggccct cgccagcctt
     6541 cgcaacgaca gcgatatagc cgtgcgaaac atggctgaag cggctatctc gaaacttcgc
     6601 ggccctaaaa aaccctagag gccgttcaag tcgtaaatac gtttttttaa aaaaactcat
     6661 tcctgcccgc gggtgatttt cgcacctatg gaggcgagct ttgtttctat tttttcatat
     6721 ccgcggtcaa tgtgataaac gcggcgaact tcgcttctgc cgcgggcgac catgccggcc
     6781 atcaccaatg cagccgacgc gcgtaaatcg ctggccatca cgggtgcggc gatgagttcc
     6841 ggcacgccgg taactatcgc cgtcgggcct tccttgcgga tatccgcacc cattcgcata
     6901 agttccggca catgcatgaa gcggtcaggg aagatttttt cggtaattgc actgttaccg
     6961 tcagccagac atagcagtac catcaactgt gcctgcaagt ccgtaggaaa ccccggatac
     7021 ggctgcgtag tcacgtcggc aggccgaatt ctatctggcc ggctgacttt taccgtaccg
     7081 tcggaatttt ccagcgccac gccgatattc cgcaacttgt caacgagtgc gagcaggtgg
     7141 tcgattcgca cgtttttgac gcagacgtct ctgccgcaaa gcgcgccggc aagcatgaaa
     7201 gtgccagctt cgatgcggtc aggaattatc ctgtgttcga cgccgttcag cgaatcgacg
     7261 ccggagatga ttatccgagg cgtgccggct ccggagattt ttgcgccggc tttgttgagg
     7321 aaatcagcca catcggcaac ttccggctcg caggctgcgc actcgatgag cgtcgtaccg
     7381 cgggctaacg tagccgccat catgacgttg agcgttccca atacggtcga gccgaatgcg
     7441 ccgcccaaaa atatctcggc gccgcgaagt cgggcggtac tcgcaatgat gtcgccgcct
     7501 tcgagatgaa tttccgcgcc aagttctcgc atgccgcgca aatgcaaatc caccggcctt
     7561 gtgccgattg cacagccgcc aggcatcgat accctcgcgc tgcgacgctt ggcaagcagc
     7621 ggcccgagta cgcatatgct ggcacgcatc tttcgcacgc ggtcatacgg agcatgcgaa
     7681 agcgattcgt ccgtgacttc aatctgcagg gaatggccgc tacgttccac gctgacgccc
     7741 agctcagcca taaggtcaac catcgcgtga atgtccgcaa ggtcgggcac atcgtgaagc
     7801 acgcttctgc cgtcacatag catcgtagcc gccagcatcg gcagggcgga attcttcgca
     7861 ccgccaactt caatattgcc gctgagttct gttccacctt ctataatgaa cttgtccata
     7921 atttttcccg ctcgatttca tcattgattg acaaaggtat catcttaaaa aatcagacgc
     7981 gtttcgcgac ggcgacccgc tcgataccct gaaaatcctt aaaaatttcc ggttctgcga
     8041 attcgcctgt ctgcactatc aggtttcgga cggcgtctgc atgtgtatat ccgaattcca
     8101 cgcaaaagat tccgccgcgg ccaagccttt tcggcacagc cggaatcagc cgccgcaata
     8161 cgtccaagcc gtcgataccc gcataaagag catcgcgcgg ctcatatttc gtcgagtcac
     8221 cgacagggtc attttctgcg acatacggcg gattggcagt tatcacgtcg aagtattcat
     8281 cattatatcg cgagccgaca gggagttcca gcaggtcagc ctgttcgaac gtgacgtttt
     8341 gcgacaggtt cagacggctg gcgttttcca cagcgacggc aatcgcggct ggggaaatgt
     8401 cgctcgcgag cacattcacg ccaggtgcat ttgcggcaat cgcacacgca acacaaccgc
     8461 tcccggtaca gacgtcccag acgttgccga catactgcga cctgtttttt ttcggctgaa
     8521 aggccggctc ggaagattgc gattcttccg gctgcgcgga ggcgagtttt tcaatctcgt
     8581 ccaaatcgac cggcgaagat tcctgcgaat cgaatttctg ctgcgtcacg tgaacgccgg
     8641 cgagcttctg aagatgctcg acggcttttt cgacaagcat ttccgtttcc ggccgcggta
     8701 tcagcacggc cggcgtaacg cggaaattca acgaataaaa ttcccggctg ccgacgagat
     8761 acgcaaccgg ctcatgcatg gctgcccgcc tgacaagttc gcgaaactgc gaacgctgcc
     8821 ggtcggtcgg ctcggagttg aatcgggcat agagctctat gcggctgcag ccgacgcaat
     8881 gagacagcag cacctccgca cacagcctgg ggttttcaag gccgctgtgt tcaaaatatt
     8941 ccttcgtcca tgaaagaagg cgacctgtcg tccatgattc gttcaagtgt tcctcactat
     9001 ttcagccgac tgctcttcgg tatcagactg gccggcggca cgcaacgcac gatagaaaca
     9061 ccagcccagc ccgcatatga ttatgcccgc catcaggaac aatatcaaat aactgtcgcc
     9121 gtcgagttgg ccgttatatt caagcggtct gattttcacg tcgctggcca tggcccacaa
     9181 cgccatgccc aaccctgccg tacaacccaa caacattatc gacaacgcgc cgccgatacg
     9241 caaaaccatg ctggcgagaa ttttcctgct ttcgtaccag accatgccga cgccggctat
     9301 cactgtggct atcaggcaga acgaaccgcc ctttaacagg agtacgccgg ctaaagcagc
     9361 cttggctgca acagcccccg catcacccat atgatatttc tgaatatgca atatcgaaat
     9421 gagccgcaaa aacgcaagcg ccagaaaaaa aaggccatcc agcgaaagca tcaccgccac
     9481 tatcccgtaa acgcggccat gaggggtttt gatgagatat tcaggccgaa taggttcatc
     9541 gtgcgttttt ttgaaattta tcgacgttat tacgaccgcc agcacaggcg cagccgccat
     9601 caccgccagg cctgcaatat tgccgatatt caatccgccg ccggcgtcgc gcagataata
     9661 aggattgccg aattcatcga gcagattcca cgcgaaaaga gctgccatca cgacaggaat
     9721 tattatccgc atggaaaaat cccaccacac gccaagtcgc cagtcactgc gttcattggc
     9781 atgttcgcgc agccgcctga gccgtatcag ccagccgagc accaaaccct cagccagcgc
     9841 cgtcagcgtt atgccccatg tgccgttgat gtaatcgtca aaaacgccca gccagttcag
     9901 cccgccctgg cagcagtaaa tcagtcccac gcccaaaccc agcagcgaaa gaatcaccag
     9961 cgtccagttc ttgctccagc cggttttgtc aactatgctc gccagagtcg actcggtaat
    10021 cgagaacgcc gaatcgatgc ctagcaatat gagcgcacga aaaaatccca ccgcgaaata
    10081 tggggcat
//
LOCUS       DAONFI010000039        10067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7610_length_10067_cov_4.077907, whole genome shotgun sequence.
ACCESSION   DAONFI010000039 DAONFI010000000
VERSION     DAONFI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7610_length_10067_cov_4.077907"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLK08_02190"
     CDS             complement(<1..399)
                     /locus_tag="PLK08_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52338.1"
                     /translation="MPSAAKKSHASSLQRLIFLSGTPQKNTELYGRNTDSPTTSEFHD
                     EYRLPSIPDLQLKFPLVSVCHPRLRKVYPQCPLESKAKMPFVATFYPHVIHMRKNREN
                     DYVFALFSRIGNWRNRLSTAAAACLVVLEIF"
     gene            499..1878
                     /gene="dnaA"
                     /locus_tag="PLK08_02195"
     CDS             499..1878
                     /gene="dnaA"
                     /locus_tag="PLK08_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPS52339.1"
                     /translation="MGKGYNNMAVVERSCWQKIIQDVVANGGNIIRPWFTELDPVCLE
                     HGLLEIKAPGLNEKNYCEHFAVRLFTEAAQKATGHLVGVCFLAGNEISDEDNDTSTPL
                     PPQNPSAAETATAERPAAVKLCKEYVFENFVTGPCNRLAHAASLAVCEGLGKTYNPLF
                     LHGASGLGKTHLLQAICHRVLGELPDAKIEFLTCEMFVNHFIDAVEKGKLHEFRYRYR
                     HVDLLVIDDIQFLADHEQSQEEFFHTFNTLYQDQKQIILSSDRGPQEIDLEERLVSRF
                     NWGMVTRIDRPCYETRVAIIHKKARQRGIALDEDVVCFLASSIDTNTRELEGALAKVT
                     MLSQVMNQPIDITIAADALGKEATPKKHDITIDDILKAVTRRFNVRLTDLQSHRRTRS
                     VALPRQVCMYLARRMTRHSLEEIGGYFGGRDHTTVMHANKSIENLRGQDPQFQATLEK
                     ITDEITAGL"
     gene            2131..3231
                     /locus_tag="PLK08_02200"
     CDS             2131..3231
                     /locus_tag="PLK08_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPS52340.1"
                     /translation="MDINRKKLEEFLRANRPAMLRDIAEIAGCSLAAVSTTLNNSSST
                     TGVSAAKRQLILKAAELVSYRPNFAAKSLVSRQSKTIGVCLRRTARYANLGPFYEAPM
                     LQGIEQAARRSDYDILLVDNRDCVKKFAENRMDALILLPGLYARKELNDIIAASPNII
                     AIDYAPNDRIDAVIFDNFAAVRLAVEHLAGLGHRRIGFIGNLHENPMADVTTRCDAFL
                     EIAQKMSLPTDGMVFNLSLWRKSIHTNQDYCSIEGMTAAKYFMSRPSDVRPTAVICYN
                     DVVASGAINAFTQAGLNLPGDMSIVGIDNSQICSFLKPALTSIRHPLEEMGELAVQRL
                     IEKLAPTNMEYSKSLTVLQPELVVRNSTAGPK"
     gene            3261..4262
                     /locus_tag="PLK08_02205"
     CDS             3261..4262
                     /locus_tag="PLK08_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019420855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPS52341.1"
                     /translation="MTQPSKNIPNIPWEEKPSDCIDAIWRYRANPVIPRDLIPSSNSI
                     FNSAVVPFEGAFAGVFRCDNKARQMRLHAGFSKDALNWQINPEPIIFNTQGKELPPFQ
                     CGYDPRVCWIEDRYWVTWCNGFAAQPTIGVAWTHDFKTFYQEENAFLPHNRNGVMFPR
                     KINGRFAMLSRPSDNGHTPFGDIFYSESPDMTFWGHHRFVMAAGDSPWQSLKIGAGPI
                     PIETSEGWLLIYHGVLRSCNGYVYSFGACLLDLEKPWQVIARNKNYLLSPQKLYECVG
                     DVPNVAFPCAALHDAATGRIAIYYGGADTVTCMAFSTLDTLMAFVKDPNNQCVNAAM"
     gene            4356..4514
                     /locus_tag="PLK08_02210"
     CDS             4356..4514
                     /locus_tag="PLK08_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52342.1"
                     /translation="MSNYGKNRFGVFTSATCGPRNFYAVNFAVVAAAAAAVAAVASFM
                     ATTPPVIS"
     gene            4601..5827
                     /locus_tag="PLK08_02215"
     CDS             4601..5827
                     /locus_tag="PLK08_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPS52343.1"
                     /translation="MQLLTSTQTDEDIERKFSRRISMLRRSCVRDIMSQTGCGSKLIN
                     FAAGQPDPSLFPNKTISRISADILADAHEQPLQYSSTEGYAPLREFITENFLGDMGIK
                     ASAEQIILTNGSQQGLDLLGKVFCDPGDTLIMDAPTYMSAIQAFELYQPRFRTLPIWE
                     DGPEIAELRHILRRGPAKFYYTISNFHNPTGVTASLGNRRQTAKLLNETGTLLIEDDP
                     YGRLCFDEECRMPTITSLSNGKNCVMMGTFSKILAPAFRVGWIFAAKEIISRLGVAKQ
                     AGDLCSNQFAQRIVWRFLKEHDIESHIVSLCERYRQRRDWMIECMAKFLPCEVHYKKP
                     GGGMFIWAELPPHLSATELLQRTLKHGIAFAPGASFFAETPIESSLRINFTYEDRQNT
                     KKGIRIIGEEIRKMLK"
     gene            complement(5866..6192)
                     /locus_tag="PLK08_02220"
     CDS             complement(5866..6192)
                     /locus_tag="PLK08_02220"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HPS52344.1"
                     /translation="MLKPIQKWLDNHKNPTSFWLHMIGVPACFIVAPTFLILSSFDGQ
                     SLYVPALMMFIGGYAIQFIGHYIEGNRSGEEKLIRRLMKIDDDKRLRDSFFRRKAADI
                     QNDSAE"
     gene            complement(6375..7196)
                     /locus_tag="PLK08_02225"
     CDS             complement(6375..7196)
                     /locus_tag="PLK08_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPS52345.1"
                     /translation="MIATVAKLGAIVSMLLAEFGDFCTFCGHTMRWSFAVLFEKRYRA
                     ELLEQFYFVGTRSVVVVMVTGLFVGAVLAVQTVPQFKAAGMVEQMGSVVNLSVLRELG
                     PVLAGVMLAGRVGGGLTAELGTMRVTQQIDALRAMGMDPLAVLVTPRFLACLLLIPVL
                     AFYASFMGILGGYFISIGLYGVDGGAFWELASRMVGVYDIFYGPIKCLFFGAAMSLIC
                     CYKGFNCKPGAAGVGRACTESFVVSCMALLAIDLLLGMTLNTIIELVWGIKSMLA"
     gene            complement(7197..8993)
                     /gene="lepA"
                     /locus_tag="PLK08_02230"
     CDS             complement(7197..8993)
                     /gene="lepA"
                     /locus_tag="PLK08_02230"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPS52346.1"
                     /translation="MDKQYIRNFSIIAHIDHGKSSLADRMMLRCGNITSREFHEQMLD
                     SMDIERERGITIKASAVTMEYELNGRKYMLNMIDTPGHVDFHYEVSRSLAACEGGLLV
                     VDAAQGVEAQTVANAHLAERCNLALLPVINKIDLPAARPEDVAMEVENLIAFPAEECI
                     FTSAKTGAGTDDMLRGIVERLPAPQGDPSSKTRALIFDSQYDEYRGVVVFVRVFDGTL
                     SVGDTIKMMGTKEEYIVTELGKLTPKPKLVKQMSAGEVGYLVGNIRSVQAIHVGDTIT
                     QAADPAEKPLPGYQPPKQMVYCDFYPGPNTQFPVMRDALEKLQLNDSALTFQAISGDA
                     LGFGFRCGFLGMLHMEIVQERLEREFDIEVVQTAPTVPYEVLTTKGEVIEVDSAGDMP
                     DVNYIQEIREPYIHAELIVPEDSIGNVMQLAEERRGTYKKTEYLSSDRVILIYEFPLA
                     EIIYDFYDKLKSATRGYGTMDFEIIGYYPDDLVKLDILVNNQRVDPLSIIVHRSKADR
                     RGRNIIQQLRSEISRHLFEIPLQAAIGTRIIARETIKALRKNVTAKCYGGDVSRKRKL
                     LEKQKAGKKRMKMIGNVEIPQEAFMAVLDTGE"
     gene            complement(9236..>10067)
                     /locus_tag="PLK08_02235"
     CDS             complement(9236..>10067)
                     /locus_tag="PLK08_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52347.1"
                     /translation="VGEVGYGEQRLLSHAIQSALSRYAWKQDAVPEMQQLVSAMRVFS
                     SYPRVDEVLGPSINGWLTMQPNPKIETVGEAIKDLSHWDPLVRVTAATFLANKMRSDP
                     QVGQEVLKQLGDPRDEMKAAAASVFSYALSYEPKKIIPIMVNILLESRSVPAQKMASE
                     SLIAHSEEAQLSAGMLLEALESRRPKPGPMRTDSILTTLSYLVHDQTPPSIQQRLLTV
                     AVNNLEFAPMGALRAIEALGPAAKSALPEIRKYRSEKADRFTRQYIDTHVLEAIEPQD
                     "
BASE COUNT         2571 a         2723 c         2473 g         2300 t
ORIGIN      
        1 aaaaatttcc aacacaacca aacaggcggc tgcggctgtt gataacctgt tgcgccaatt
       61 tccaattcga gagaaaagag caaaaacata atcgttttca cgattttttc gcatgtggat
      121 aacatgtgga taaaaagtcg caacaaatgg catttttgct tttgactcca gcgggcattg
      181 tggataaact ttcctgagtc gcggatgaca tacgctaacg agagggaact tgagttgcaa
      241 gtcagggatt gacggcaggc gatattcatc atggaattcc gaagtggtcg ggctgtctgt
      301 gtttcgcccg tacagttcgg tatttttttg tggtgtaccc gacaaaaata tcagccgctg
      361 gaggctggag gcatgagatt tttttgccgc agacggcaaa accgtctctt tgaaatgagt
      421 taataatttt cgttccggca gaggcggagt tgctcgcgat ttgtgtctcc gctttcaatg
      481 ccggccgacg gcgagagaat gggaaagggc tataataaca tggcagtggt cgaacggagt
      541 tgttggcaga aaatcatcca ggatgtagtg gcgaacggcg gcaatataat caggccatgg
      601 tttacggagc ttgacccggt ctgcctcgaa cacggcctgc tggaaataaa agcccccggc
      661 ctcaacgaaa aaaattattg cgaacacttt gccgttagat tattcaccga agccgcccaa
      721 aaagccaccg gccaccttgt gggcgtctgt tttctggccg gcaatgaaat ttccgatgaa
      781 gataacgata cttccacccc cctgccgcca cagaacccat ctgccgccga aactgcaacc
      841 gccgaacggc cagccgcggt aaaactgtgc aaagagtatg tttttgagaa tttcgtaacc
      901 gggccctgca accggctggc ccacgctgcc agccttgcag tatgcgaagg cctcggcaaa
      961 acgtacaacc cgctcttcct gcacggcgca agcgggctgg gaaagacgca tctgctgcag
     1021 gcaatctgcc accgcgtact cggcgaactg ccggatgcga aaatagaatt tttaacctgc
     1081 gagatgttcg tcaaccattt catcgacgcc gtcgaaaagg gaaaattgca cgaattccgc
     1141 tatcgttatc gtcacgtgga tctgctggta atcgacgaca ttcaatttct cgccgaccat
     1201 gaacaaagtc aggaagaatt tttccatact ttcaacaccc tatatcagga ccagaagcag
     1261 attattctct caagcgaccg cggcccacag gaaatcgacc tggaagaacg gctcgtcagc
     1321 cgtttcaact ggggcatggt tacgcgaata gaccggccct gctacgaaac gcgagtcgcc
     1381 atcattcaca aaaaagcccg ccagcgcggc atcgcgctgg atgaagacgt cgtgtgtttc
     1441 cttgcaagtt caatcgacac caacacgcgg gagctcgaag gagcgctggc taaggtgacc
     1501 atgctctcgc aggttatgaa tcagccgata gatatcacca tcgccgccga cgcgctgggc
     1561 aaggaagcga cgcctaaaaa acacgacatc acaatagacg acatactcaa agcggtaact
     1621 cgccgtttca atgttcgcct tactgacttg cagtcgcatc gcagaacgcg cagcgtcgct
     1681 ctgccgcggc aggtatgcat gtacctggcc agacgaatga cccgtcacag ccttgaagaa
     1741 atcggcggct atttcggagg ccgcgaccat acgactgtaa tgcatgcgaa caaatccatc
     1801 gaaaatctgc gaggccagga cccgcagttt caggctacac tggagaaaat aaccgacgaa
     1861 ataaccgcag gcttatgaaa ttcgcaaatt tggcaaaaac tgttttatat aaaaaactta
     1921 aaaaaatttg aaaataacgg acgtgtgcct tcttaaatgg gctacgctgg gagtttcgga
     1981 acggcagcct tgttcggcaa taaccgaaca gggctgtttt tttatttttt gtatcgaacg
     2041 gttctcaaaa agaaatttct ttcgcgaacc actctaattt cgattgacac catatgaaac
     2101 gattaatatg atgatatgaa atgtttcatt atggatatta acaggaaaaa actcgaagaa
     2161 ttcctcaggg ccaatcggcc agcgatgctt agggacatcg cggaaatcgc cggctgctcg
     2221 ctagccgccg tttccaccac cctaaataat tccagcagca ccacaggcgt atctgccgcc
     2281 aagcgacagc tgatactgaa agcagctgaa ctcgtcagtt accggcccaa cttcgccgca
     2341 aaatcgctgg tcagccgcca gagtaaaact atcggcgtat gcctgagaag aaccgccagg
     2401 tatgccaacc tcggcccgtt ttatgaagcc cccatgctcc agggcattga acaagccgca
     2461 cgtcgcagcg attacgatat tctgctcgtc gacaaccgcg actgcgtaaa aaaatttgcg
     2521 gaaaaccgca tggatgccct gatattattg cctgggctat acgcaagaaa agaactgaac
     2581 gacatcatcg cagcctcgcc gaatataata gcgatagact atgccccgaa tgacagaatt
     2641 gatgctgtga ttttcgacaa tttcgccgca gtacggctgg cagtagagca tcttgcaggc
     2701 ctgggccaca ggcgaatagg tttcatcggc aatcttcacg aaaatccgat ggctgacgtg
     2761 acaacccgct gcgacgcttt tctggaaatc gcccaaaaaa tgtcgctgcc gacagacggc
     2821 atggttttca atctttcgtt atggcgaaaa agcatacata ccaatcagga ttattgttca
     2881 atcgaaggca tgacggcggc gaagtatttc atgtcgcgtc cgtcagacgt ccggccgacc
     2941 gccgtgatat gctataacga cgtggtagcc tccggtgcga taaatgcatt cacgcaggct
     3001 ggcctgaatc tgcccggcga catgagtata gtaggaatag ataattcaca gatatgcagt
     3061 tttctgaagc cggcacttac gtccatacgt catccgttgg aggagatggg cgaactcgca
     3121 gtacagcggc tgattgaaaa actcgctcca accaatatgg aatattcaaa atccctgaca
     3181 gtattgcagc cggaattggt agtgcgtaat tctaccgccg gcccaaaata gttttttaca
     3241 tgaaaaagga aagccgtcag atgacacagc cgtcaaaaaa tatcccgaat atcccctggg
     3301 aggaaaaacc ctccgactgc atcgacgcca tatggcgtta cagagcaaat ccggtcatac
     3361 cccgcgatct tataccctcg tcgaacagca ttttcaattc tgccgtagtg cctttcgaag
     3421 gcgcattcgc cggcgtattc cgttgcgaca acaaggcacg ccagatgcgt ctgcatgcgg
     3481 gattcagcaa agacgcctta aactggcaaa tcaatcccga gccgataatt ttcaacaccc
     3541 agggcaaaga gcttccgccc ttccagtgcg gttacgaccc tcgagtctgc tggatagaag
     3601 accgctactg ggtcacgtgg tgcaacggat tcgccgctca gccgactatc ggagttgcct
     3661 ggacgcatga ttttaaaacc ttctatcaag aggaaaacgc ctttctgccg cacaatcgca
     3721 acggcgttat gtttccgcga aaaatcaacg gccgcttcgc catgctcagc cgcccaagcg
     3781 acaacggcca cacgccgttc ggcgacattt tttacagcga aagtcctgac atgacattct
     3841 ggggccatca tcgtttcgtc atggctgccg gcgattcgcc atggcagagt ctgaaaatcg
     3901 gcgccggccc cataccgata gaaacatccg aaggctggct gctgatttat cacggcgtac
     3961 tcagaagctg caacggctac gtttacagtt tcggcgcatg tctgctggac ctggaaaaac
     4021 cgtggcaggt aatcgccaga aacaaaaatt atctgctcag cccacagaaa ctttatgaat
     4081 gcgtcggcga cgtaccgaac gtagcatttc cgtgcgctgc gttgcatgac gccgcaaccg
     4141 gcagaatagc catatattac ggcggagccg acaccgtcac ctgcatggcg ttttcaacgc
     4201 tggacaccct gatggcgttc gtcaaagacc cgaacaatca gtgcgtcaac gccgcaatgt
     4261 gaggcggcga tattccggaa gtttgtctga acaggaaaga tttatgttga cgaatccgga
     4321 tttacggtta ttatttttct aaggatagtc actttatgag caattacggc aaaaacaggt
     4381 ttggcgtttt cacgtccgca acatgcgggc cgcgcaactt ttatgccgtc aatttcgccg
     4441 tcgtagccgc agctgccgca gcagtagccg ccgtcgcctc tttcatggca acgaccccgc
     4501 cggtgatttc ctgaaataac acatctcgga aaaagtcaag gccttgtggg tttgttgccc
     4561 gcaaggcctt tttttattgc cacatttcac ggagaaaaaa atgcaactgc tgacatcgac
     4621 acaaacggat gaagatatcg aacgaaaatt ctcgcggcgt atatcaatgc tgcgccgttc
     4681 gtgcgtacgt gacatcatga gccagaccgg ctgcggcagc aaactcatca acttcgccgc
     4741 cggccagcca gacccatcgt tgtttccaaa caaaacgata tcgcggatat cagccgatat
     4801 cctggcagac gcacacgagc agccgctgca atattcgtcg acggaaggtt atgcgccgtt
     4861 gagggaattc atcactgaaa attttttggg cgatatgggc ataaaggcat cggcagaaca
     4921 gataatcctg accaacggtt cgcaacaggg tcttgacctg ctgggcaagg ttttctgcga
     4981 ccctggcgac acgcttataa tggacgctcc gacgtacatg tcggcaattc aggcatttga
     5041 actgtatcag ccgcgatttc gtacgctgcc gatatgggag gacggcccgg aaatcgccga
     5101 acttcgacat attcttcgcc gcgggccagc aaaattctat tacacgataa gcaattttca
     5161 caatccaacg ggtgtgaccg cctcgcttgg aaacagaagg cagacggcga aattgctgaa
     5221 cgaaaccggc acgcttttga tagaggacga tccatacggc agattatgct tcgacgaaga
     5281 atgcagaatg cccacgatta catctctgag caacggcaaa aattgcgtca tgatgggtac
     5341 gttttcaaaa attctcgcac ccgcattccg cgtcggctgg atatttgcgg caaaagaaat
     5401 catttcgcgt ctgggcgtcg ccaagcaggc cggcgacctg tgctcaaacc agttcgccca
     5461 gcggatagta tggcgatttc tcaaagaaca cgacatcgag tcgcatatcg tcagcctctg
     5521 cgaacgatat cgccaacgac gcgactggat gattgaatgc atggcaaaat tcctgccttg
     5581 cgaagttcat tacaaaaaac ctggcggcgg aatgttcatc tgggcggaac tgccgccgca
     5641 tctgtccgcg acggaactac tgcaacgtac gctcaaacac ggaatcgcgt tcgcaccagg
     5701 cgcgtcgttt tttgcggaaa cgccgattga atcgtcgttg cgtatcaatt tcacatacga
     5761 agaccggcag aacacaaaaa aaggcatccg tataatcggc gaggaaataa gaaaaatgct
     5821 gaaatgaaaa acagccagct tcaacgttaa aatcgtatat gacgattatt cagcggaatc
     5881 gttttggatg tcggcggctt tgcgccgaaa gaaggaatcg cgcagacgct tgtcgtcatc
     5941 gattttcata aggcgacgga tgagtttttc ctcgccgctg cgattgccct ctatgtaatg
     6001 gccgatgaac tggatggcat aaccgccgat gaacatcatc aacgccggca cataaagcga
     6061 ctggccgtca aaggacgaca atatcaaaaa tgtcggcgca acgataaaac acgccggcac
     6121 gccaatcatg tgcagccaga aactcgtggg attcttatga ttatccaacc atttttgtat
     6181 aggttttagc attttacctt ttaaatccaa aaatcgaaca ttactcattc aaagttaaca
     6241 aaaatcaaaa acaagttaca agcaagtttc agtttccaag aatcaggaga attacggcta
     6301 ctgttcttga aatttatttt aaaaaattgt tgcttgtaaa ttgaatgtct tgctacttga
     6361 aactttttct tttgctacgc cagcatggac tttatgcccc acaccaactc tatgatggtg
     6421 ttaagtgtca tgccgagcaa caggtcgata gccagcaaag ccatacagct tacgacgaaa
     6481 ctctccgtac atgctctgcc cacgccagcc gcgcccggtt tgcagttaaa acccttgtaa
     6541 cagcatatca gcgacattgc cgcgccgaag aacaggcact ttatagggcc gtaaaagatg
     6601 tcgtacacgc cgaccatcct cgacgccagc tcccagaacg ctccgccgtc cacgccatat
     6661 aaaccgatgc ttatgaaata tccgccgagt atgcccataa agctggcata aaacgccaat
     6721 accggaatca gcagaagaca cgccagaaat ctcggcgtaa ccagcaccgc caacgggtcc
     6781 atacccatag ctctcagggc atcaatctgc tgcgttaccc gcatagtgcc caattccgcg
     6841 gtcagtccgc cgccgacccg gccagccagc attacgccgg cgagcaccgg cccaagttca
     6901 cgcaaaaccg acagattcac gaccgaaccc atctgttcga ccatgccggc ggctttgaac
     6961 tgcgggacgg tctgaaccgc cagcaccgct ccaacgaaca ggccggtaac catcaccacc
     7021 accacgctgc gcgtgccgac gaaataaaac tgttccagca attcagccct gtagcgtttc
     7081 tcgaacagca cggcgaaact ccaccgcatc gtgtgcccgc agaatgtgca gaaatcgccg
     7141 aactccgcca ggagcatgct gacgattgcg ccaagttttg cgactgttgc aatcatttat
     7201 tctccagtat caaggacagc catgaatgct tcctgaggta tttcaacgtt gcctatcatt
     7261 ttcatgcgtt ttttgccggc tttctgtttt tcgaggagtt ttcgcttgcg gctgacatcg
     7321 ccgccgtagc atttggcggt aacgtttttc cgcagcgcct ttatcgtttc cctcgcaata
     7381 attctggtgc cgattgccgc ctgcagcgga atttcaaaca gatgacgcga tatctcgctt
     7441 cgcaactgct gtatgatgtt cctgccgcgg cggtcagcct tcgaacggtg aactatgata
     7501 ctcaacggat ccacccgctg attgttcaca agtatgtcca gttttacgag gtcgtctgga
     7561 taatatccta taatctcgaa atccatcgtt ccataaccgc gggtggcact cttgagcttg
     7621 tcgtaaaaat cataaataat ctcagccagc ggaaattcat aaatcaaaat cacgcgatcg
     7681 ctggaaagat attcggtttt tttgtaagtg ccgcggcgtt cttcggcaag ctgcatgaca
     7741 ttgccgattg aatcttcagg cacaataagt tccgcgtgaa tgtacggctc gcgaatttcc
     7801 tggatatagt ttacgtccgg catatcgccg gcggaatcga cttctataac ctcgcccttt
     7861 gtggtaagga cttcatacgg cacagtcggc gcggtctgaa cgacttcaat gtcgaattcg
     7921 cgttcaagcc gctcctgcac gatttccatg tgcagcattc ccaggaatcc gcatcggaag
     7981 ccgaacccaa gcgcatcgcc gctgatagcc tgaaacgtaa gcgccgaatc attaagctgt
     8041 aatttttcca gcgcatcgcg catgactgga aactgcgtgt tcgggccggg ataaaaatcg
     8101 cagtagacca tctgcttggg cggctgatag ccggggaggg gcttttctgc cgggtctgca
     8161 gcctgcgtaa tggtatcgcc gacatgtatc gcctgcacac tgcggatatt gcccacgagg
     8221 taaccgactt cgccggcaga catctgtttc acgagtttgg gcttgggcgt aagtttgccc
     8281 agttcggtta cgatgtattc ttccttcgtg cccatcatct ttatcgtatc gcccacgctc
     8341 aatgtgccgt cgaacacccg cacgaaaaca actacgccgc gatattcgtc atactggctg
     8401 tcgaaaatca acgcgcgggt tttggaactg gggtcaccct gcggggcagg cagccgttca
     8461 actatgccgc gaagcatgtc atccgtgccg gcacccgtct tggcggatgt gaatatgcac
     8521 tcctcggctg gaaacgcgat aaggttttcg acttccatcg ccacgtcttc tggcctggca
     8581 gccggcagat ctatcttgtt gataaccggc agaagcgcaa gattgcaccg ctcggcaaga
     8641 tgggcgttgg caacggtctg agcctccact ccctgagccg catccaccac gagcaaaccg
     8701 ccctcgcagg ctgccagaga acgactgact tcgtaatgga aatcgacgtg gccaggcgta
     8761 tcaatcatgt tgagcatgta tttcctgccg ttcagctcat actccatcgt gacggctgac
     8821 gcctttatgg taatgccgcg ttcacgctct atgtccatgg aatcgagcat ctgctcgtga
     8881 aattcccggc tggtaatgtt tccgcaccgc aacatcatac ggtcggcaag cgaacttttg
     8941 ccgtggtcga tgtgggcgat aatactgaag ttgcgtatgt actgcttatc catatttttt
     9001 taatatatcc ttcgtaattc tacggttctt agaatgtctg ccgaaagaat atttttttaa
     9061 agaacagcta taaaccgcag ataaaataac catgtgaaag caaactgaca aaatcgccga
     9121 atacactctc tccacatcat tagccgaaaa ccggattgaa cagacccgcc cgactgacat
     9181 ggtgcggctg catattttct cgacagatat aaccgccgtg cgacagatat atccgtcaat
     9241 cctgcggctc gatagcctcc agaacgtgcg tgtcgatata ctgcctggta aagcggtcag
     9301 ccttttccga acgatatttc cttatctccg gcagtgccga cttagccgcc ggccccagtg
     9361 cttcaatcgc acgcaacgca cccatcgggg cgaattccag attgttaacc gcaaccgtca
     9421 acagccgctg ttgtatgctc ggcggcgtct ggtcgtgcac gagataactc agcgtcgtga
     9481 gaatgctgtc ggtgcgcatg gggcccggct tgggccgtcg tgattccagc gcctccagca
     9541 gcatgccggc ggaaagttgt gcctcttctg aatgggcaat gagcgactcc gacgccattt
     9601 tctgcgccgg cactgatctg ctttccagaa ggatgttgac cattataggg atgatttttt
     9661 tcggctcata gctcagggcg taactgaaaa cgctggcggc ggcggctttc atttcgtcgc
     9721 gggggtcgcc aagctgcttg agcacttcct ggccgacttg cgggtcgctg cgcatcttgt
     9781 tggcaagaaa agttgccgcc gtcacacgga cgagcggatc ccagtggctg aggtctttta
     9841 tggcttcgcc gacggtttcg attttcggat tcggctgcat cgtcagccag ccgttgatgg
     9901 acggcccaag cacctcgtca acgcgaggat acgaactgaa cacgcgcatc gccgagacga
     9961 gctgctgcat ttccggcacg gcgtcctgct tccacgcata gcggctgagt gcggactgga
    10021 tggcgtggct taacagccgc tgttcgccat aacccacctc cccgacg
//
LOCUS       DAONFI010000040         9985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7686_length_9985_cov_3.942900, whole genome shotgun sequence.
ACCESSION   DAONFI010000040 DAONFI010000000
VERSION     DAONFI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7686_length_9985_cov_3.942900"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLK08_02240"
     CDS             <1..635
                     /locus_tag="PLK08_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPS52348.1"
                     /translation="IRRPKLVWRKKYWLFYVLAFLFGARKQLFMTFGPWVLIRIFNEP
                     ASTIAKLWIAGSMLQVFFQPYLGRLIDRWGERTVLLCDSAILMLICAGYGLSHFIGNP
                     RTGLWLLYVCYVGDMMMFGVNMARSTYVAKIAPQPEDVSPTLSMGITINHAVSMSLPW
                     LGGWIWDRFGHSMVFAGAAGIAVLMAIFSAMIPRKSILNEMEKFHAPKTA"
     gene            880..1173
                     /locus_tag="PLK08_02245"
     CDS             880..1173
                     /locus_tag="PLK08_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPS52349.1"
                     /translation="MASKLYVGNLSFSVTSDDLEKIFGEFGQVEDATVITDRQTGRSK
                     GFGFVTLSSDEAAQAAIAATNGKEIAGRAATVNIAKPREDRPARGGYSNRDRR"
     gene            complement(1275..3764)
                     /locus_tag="PLK08_02250"
     CDS             complement(1275..3764)
                     /locus_tag="PLK08_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPS52350.1"
                     /translation="MKTIHLICNAHLDPVWQWPWEEGAAEAVSTFRVAAEFCEKFDGF
                     VFCHNEVVLYEWIEEYEPQLFKKIQKLVKAGKWHIMGGWYLQPDCNMPAGESFVRQIL
                     LGREYFWKKFGKRPTTAINFDPFGHSRGLVQIMAKCGFDSYLFWRPNNTDCPLPDDLF
                     IWEGFDGSRVTGLRQSLGYNTCLGKAVNKIKDFMAGNYRHDTGLLLWGIGDHGGGPSH
                     EDLQKIAMLIKDTPAIKIFHSTPEAFFKQVKHEEKNLPVVSKSLNAWAVGCYTSQVLI
                     KQLNRKVENELFATEKMLTSAWKQGVLAEYPADELYKVMRDMAFLQFHDIFPGSSVEE
                     VEETSIRLANHALEELSRLKARAFFSLASGQKKATNGYIPILVYNPHPFRVNTTVECE
                     YQLEDVKFLKDGFHIGVACMNGRELPSQMEKEASSIALDWRKKVVFNAILEPSQMNRF
                     DCKLIKIAARPTLAIKAKNGQFRFKTKDVEVVINAKTGLMDKFRVNGKDMVGKSAFLP
                     LVVADNEDPWGMLVDRFRKVVGRFRLLSAQKSAEFSGLKAKKFPPVRVIEDGDVRTVI
                     EAVFGYNDSFICQHYILPKRGTEVGVTTRVIWGEKNKMLKLDIPVAMKGTPKVFGQVA
                     YGVEELPADDRELVAQKWSAVANDSDAITLINNGVYGFDFVKGSLRPTLLRSASYCSH
                     PIENEPQIIPQDRFLPRIDTGQRIFKFLLNAGTKSERFAKVDRESIVYAEKPMTLSFF
                     PCGDGKKPGRFVTLSDDVIELSAMKKAQKGDGVIIRLFNPTDKKRKTTLSVPAMKINR
                     KLELTPFEIKSFNADSKSGRLTEITPDERPL"
     gene            3940..5199
                     /locus_tag="PLK08_02255"
     CDS             3940..5199
                     /locus_tag="PLK08_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPS52351.1"
                     /translation="MQPYGGVNYDIAIIGAGAAGQTAAIAASQTGRRVVLIEQMSQPG
                     KKLLATGGGRCNITRMRDVAFFESVFGRQGRFMNAVLNELPPQKLRDFFKSIGVATKE
                     ENDGMVYPVTEKAMTVQAALRHRYEKLGVRLITGHLAEILIVENGRLAGVELAGGMRI
                     SAPKIILACGGRSWPTLGGTGGGYALAVQAGHTIKTPTPTLVPLLVKEHWVAKLAGVA
                     LPSARVWINLPRQNKTGRTGDVLFTHKGLTGPAVLDISGDVAELLASGKECVPLQLEP
                     VAGMDASQWRKKFEMWRNECGRKHVVNLLKNFLPTAVCETILELAAIPTETTPAQLTS
                     AARDKLAEMLGCLNFTINGTEEFDIAFVTRGGVTLKEVNPSTLESRIMPGLYFAGELL
                     DLDGPCGGFNLQWAFSSGYFAGKTASM"
     gene            complement(5328..6122)
                     /gene="dapB"
                     /locus_tag="PLK08_02260"
     CDS             complement(5328..6122)
                     /gene="dapB"
                     /locus_tag="PLK08_02260"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPS52352.1"
                     /translation="MIKLAIIGAGGRMGRRIAALAIESGEFDVVGALEMSGHDDLGKD
                     VGELAGVGRFGLKVADTLGGIDFPDVAIDFSLPAGTMHWLPQFRKKGVAMVIGTTGLT
                     DSQKAELADAAKEIPIVFAGNYSMGINLLTTLVEQAVKTLGEDYDVEIVETHHRFKKD
                     APSGTAIMLAEAACDARGVELNDVAVRGRSGVCPRKTGEIGIHALRVGDTVGEHSVHI
                     GNLGETITLSHSAHTRDTFVRGALRAAKWVIGKKPDLYNMKNVLGL"
     gene            complement(6125..7516)
                     /gene="thrC"
                     /locus_tag="PLK08_02265"
     CDS             complement(6125..7516)
                     /gene="thrC"
                     /locus_tag="PLK08_02265"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPS52353.1"
                     /translation="MKYISTRGGISPIEFQDAVMMGLADDGGLVIPESIPCVAGRLAE
                     LARLSYPELAYEILHLFATDIPQKDLHDIVTRTYNPQAFEGGVAPVVPVGDVFIQELW
                     HGPTLAFKDVALQLLGNLFEYVLAHRGGRLNILGATSGDTGSAAIYGVRGKAGINIFI
                     MHPAGRVSPIQERQMTSVLDGNVHNIAVDGSFDDCQNIMKTLSADLDFKRKYSLGAVN
                     SVNWARVLAQVVYYFSGVFDVQRATGAKTVRIAVPTGNFGDVLAGWYAVKMGLPVSKL
                     ILATNENDILARFFNTGRYSIGKVFQTLSPSMDIQVASNFERYLYYRLGSDAVKLKNI
                     MDEFAQKGSLSVLPADGGIDGDFISSSANCGQVIEVIRRHFERYGYLLDPHTACGVVA
                     AEKSGIGGEPVICLATAHPAKFPAAIAEATGDASLATHPRIEKLMDMETRCVKLPGDR
                     QAVRRFIIETLGE"
     gene            complement(7838..7911)
                     /locus_tag="PLK08_02270"
     tRNA            complement(7838..7911)
                     /locus_tag="PLK08_02270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7875..7877),aa:Arg,seq:tct)
     gene            8199..9587
                     /locus_tag="PLK08_02275"
     CDS             8199..9587
                     /locus_tag="PLK08_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPS52354.1"
                     /translation="MSDIDKVFKAYDVRGIYAPGEIDEDLAWKIGHATAQFLHKSMSG
                     YDRGQASVNKIVVGYDMRPHSIPLATALMEGIASSGATCINIGMCDTPMVYFAINHLE
                     ACGGIMVTASHNPIEYNGFKISSYKAKPVGSATGLNDIRDMCKNIQRTPAPSEATPVQ
                     NVDLWADYRKHVLKFLNLPRKLKVVVDASNGMGGRMIPAIFGGVKNLELVEMNFEMGK
                     GFVHQPNPLVAANMKWVQDGVKANKADLGICLDGDADRCMFVDEKGCIIRCDIMTTWL
                     AMDFLKDNPGATIVYDLRSSKILPEKVRACGGVPRRERVGHAFMKKAMADTQAVFGGE
                     LSGHSYFRDNFYADSGAIVFARAISVLSASDKPISTQVASLCKYSHSGEINFEVEDKE
                     GKMKEIAQKFPDAEIDYLDGVTCTYPNWWCNVRPSNTEPLLRLSLEAETPALMKEKLA
                     IIEKMLGKPVDH"
     gene            9679..>9985
                     /locus_tag="PLK08_02280"
     CDS             9679..>9985
                     /locus_tag="PLK08_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52355.1"
                     /translation="MKNIIGLFVLGLIVAGCDKEVKLTFVNETRNDLPLSLKTSSGIN
                     ESLGIMRAEGSHAERTFKYKKDELPVNVTWKAGEYCGKFDITDKTNKNLRIDFLPDGP
                     "
BASE COUNT         2531 a         2629 c         2463 g         2362 t
ORIGIN      
        1 acattcgccg gcccaagctg gtatggagga aaaaatactg gctgttctac gttctggcgt
       61 ttctgttcgg agcaagaaaa cagttgttca tgactttcgg gccgtgggtg ctcatcagaa
      121 ttttcaatga gccggcgtcg accatcgcca aactctggat agccggttca atgttgcagg
      181 tcttttttca gccgtacctg ggacggctga tagaccgctg gggcgaacga acggtactcc
      241 tgtgcgattc ggcgatactg atgctcatat gcgctggata tggactgtcg cacttcatcg
      301 gcaatccgcg gacaggcctc tggctgctat acgtatgtta cgtgggcgat atgatgatgt
      361 tcggcgtaaa catggcccgc tcgacatatg tggccaaaat agcccctcag ccggaagacg
      421 tcagcccgac actgagcatg ggcataacca taaatcacgc cgtttccatg tcgctgccgt
      481 ggctgggcgg ctggatatgg gacagattcg gccattcgat ggtcttcgca ggtgcagccg
      541 gcatagctgt actcatggcg atattcagcg ccatgattcc acgtaagtca atactgaatg
      601 aaatggaaaa attccacgct ccgaagacgg catgacacga aaaacgtaaa aaaagactta
      661 agtaatgatt ttttttgcaa taatccccct gcaaaagttg acaaaaatat tttaaaaata
      721 gtaaaaatag taatttattg ttttgaaaaa gtaccgcagt ttggtaatat acttccgaag
      781 tagacaccag agttggtgtc gaacgtgttt agagcctgta ggttccgcag tcttcctctt
      841 ggccctttac ctgttagact gcgaaagaag gagcctttaa tggctagcaa actttatgta
      901 ggaaatctga gcttcagcgt tacgtccgac gatctcgaaa agatattcgg tgaattcggc
      961 caagtcgaag acgcaactgt aataaccgat cgtcagaccg gacgcagcaa gggttttgga
     1021 ttcgtgaccc tttcctcaga tgaagccgcc caggcagcta tcgctgcaac caacggcaag
     1081 gaaatcgccg gccgtgccgc aacggtcaat atcgccaagc cgcgcgaaga ccgcccggcc
     1141 cgcggtggtt attcaaatcg cgaccgtcgt taatttttca ttaaacggca taaaaaaagg
     1201 cggcatcttt aatgatgtcg ccttttttat ttccacgatt atcgtactga gcagttctcg
     1261 aaagaatttt cgctctataa tggccgttcg tccggcgtaa tttccgtaag ccggccggat
     1321 ttggaatcgg cgttaaagga ttttatctca aacggggtca gttcgagttt tctgtttatc
     1381 ttcatcgccg gaacggacaa tgtcgttttg cgttttttgt ccgtcgggtt gaataaccgt
     1441 ataatgacgc cgtcgccctt ttgcgccttt ttcatggcag agagttcaat gacgtcgtcg
     1501 ctgagcgtca cgaatctgcc gggctttttg ccgtcgccgc acgggaagaa tgaaagcgtc
     1561 atcggctttt ccgcatacac tatcgattca cggtctacct ttgcgaaacg ttcagacttc
     1621 gtgccggcgt tcagcaaaaa tttgaagata cgttggccgg tatcgattcg cggcaaaaac
     1681 ctgtcctgag gaataatctg cggctcattt tctatcgggt gtgaacaata agaagcggac
     1741 cgcagcaaag tcggccgtaa tgaacctttt acgaaatcaa aaccgtaaac gccgttgtta
     1801 atcagtgtaa tcgcatcact gtcgttggcg acggcagacc atttctgcgc gacgagttcg
     1861 cggtcgtcag caggcagttc ctcgacgccg tacgcaacct ggccgaaaac ttttggcgtg
     1921 cctttcatcg caactggtat atccagtttg agcattttat ttttctcgcc ccatatgacg
     1981 cgagtggtaa cgccgacttc cgtgccgcgt ttgggcagta tgtaatgctg acagatgaat
     2041 gaatcgttat agccgaaaac cgcctctata acagtgcgga catcaccatc ctctattacg
     2101 cgaactggcg gaaacttttt agccttcaag ccggagaatt ccgcactctt ttgagctgaa
     2161 agcagcctga atctgccgac caccttgcgg aaacggtcaa ccagcatacc ccacgggtct
     2221 tcattatccg ccacaaccag cggcaggaat gcagacttgc cgaccatgtc ttttccgttt
     2281 acacggaact tgtccatcaa tccggttttg gcatttatga cgacttcgac atctttggtt
     2341 ttgaaacgga attggccgtt tttggcttta atcgccagcg taggccgcgc ggcaattttt
     2401 atcagtttgc agtcaaaacg gttcatctgc gacggctcaa gtatggcgtt gaatacgacc
     2461 ttctttcgcc agtcaagtgc aatcgacgat gcttcctttt ccatctgaga cggcagttcc
     2521 ctgccgttca tgcaggcgac gccgatatgg aaaccgtctt tcaggaattt gacgtcttca
     2581 agctgatact cgcactcgac agttgtattt acgcggaagg gatgtgggtt gtaaaccaga
     2641 atgggaatat agccatttgt tgccttcttc tgaccggatg ccaacgagaa aaacgcacga
     2701 gccttcagcc gtgaaagctc ctctaaggca tgattcgcca aacggattga cgtttcttcc
     2761 acctcttcca cacttgagcc gggaaaaata tcgtggaatt gcagaaacgc catatctcgc
     2821 atgactttat aaagttcatc agccggatat tcggcaagta cgccctgttt ccaggcactt
     2881 gtgagcattt tttcggttgc gaaaagctca ttttccactt ttcgattcag ctgtttaatc
     2941 agcacctgcg acgtataaca gccgactgcc catgcattca ggcttttcga gacgaccggc
     3001 agattcttct cttcgtgttt gacctgtttg aaaaacgcct ccggcgtcga gtggaatatt
     3061 tttatagccg gggtatcttt tatcagcatg gcgatttttt gcaaatcttc atgacttggg
     3121 ccgccgccgt ggtcgccgat gccccagagc agcaaccctg tatcatgcct gtaattgcca
     3181 gccatgaaat cttttatttt attgacagcc ttgcccaggc acgtgttgta acccaaagac
     3241 tgccgcagac ccgtcacgcg agagccgtca aaaccttccc atataaacaa atcatccggc
     3301 agcggacaat ccgtattatt cggccgccag aacagatacg aatcaaatcc gcacttggcc
     3361 attatctgaa caaggccgcg agaatggcca aacgggtcga agttaatcgc agtcgtcggc
     3421 cgtttgccga attttttcca aaaatattca cggcccagca gtatctgccg cacaaacgac
     3481 tcgccggcgg gcatattgca gtcgggctga agataccagc cgcccatgat atgccatttt
     3541 ccggctttta cgagtttctg aatttttttg aaaagctgcg gctcatattc ttctatccat
     3601 tcgtaaagca cgacttcatt atggcagaaa acaaagccgt cgaatttttc acaaaactcc
     3661 gcagccacac gaaaagtgct taccgcctcg gcagctccct cttcccacgg ccactgccag
     3721 acaggatcga gatgcgcatt gcatataaga tgtattgttt tcattttaac gttatatcct
     3781 tttaaaaaac ttcagggatg aagctaattt acaaatttca atggaattgg caatgtttat
     3841 ttatggttaa ttgtaaagat tttgaacaat tttgttcgaa agattgagta gcggcgctaa
     3901 aagtttgtgt tggcggctct gccgtgctag actgcagcca tgcaaccgta tggtggagta
     3961 aattacgata ttgcaataat tggcgcgggc gctgctggcc agacggcagc cattgccgcc
     4021 tcgcagactg gccgtcgcgt cgtgctaata gaacagatgt cgcaacccgg caaaaaattg
     4081 ctcgccaccg gcggcggaag atgcaacatc acccgcatga gagatgtggc gtttttcgag
     4141 tccgtattcg gcagacaggg gcgattcatg aacgccgtgc tcaacgaact tccgccgcag
     4201 aaattacggg attttttcaa atccatcggt gtagcgacga aagaggaaaa cgacggcatg
     4261 gtttatcccg taacagaaaa ggccatgacc gtgcaggcag cactgcggca tcgatacgag
     4321 aaactcggcg tacggctgat aacaggccat ctggcggaaa tacttatcgt ggaaaacggc
     4381 agacttgccg gagtcgaatt agccggcggg atgaggattt ccgctccaaa aatcattctg
     4441 gcgtgcggcg gcagaagctg gccgacgctc ggcggaacag gcggagggta tgcactcgcc
     4501 gtacaagccg gccatacaat caaaactcca acacccacgc ttgtgccgct actcgttaaa
     4561 gagcactggg tagctaaact tgccggagtt gcactgcctt cagcgcgggt gtggattaac
     4621 ctgccgcgac agaataaaac aggtcgtacc ggcgacgtgc ttttcacaca caaaggcctc
     4681 accggcccgg ccgtgctgga tatttccggc gacgtagcgg aactgctcgc cagtgggaaa
     4741 gaatgcgtac cgctacagct cgaacctgtc gcaggcatgg acgcctcgca atggcgaaaa
     4801 aaattcgaga tgtggcgtaa cgaatgcggc aggaaacatg tcgtcaatct gttaaaaaat
     4861 tttctgccaa ctgccgtctg cgaaacgatt ctggaacttg ccgccatacc aaccgaaact
     4921 acgcctgctc aactgacgtc agccgcccgc gacaaactcg cagaaatgct cggctgtctg
     4981 aacttcacaa tcaacggtac ggaagaattc gatatcgcct tcgtaacgcg aggcggtgta
     5041 acactgaaag aggtcaaccc ctcaacgctt gaaagccgca tcatgccggg cctttatttc
     5101 gccggtgaac tgctcgacct cgacggcccc tgtggcggat ttaatcttca atgggccttt
     5161 tcaagcggat atttcgcagg caaaacggca agcatgtgaa atacgctttc cagcgtcact
     5221 ccggccagta gaaagtttca ataaacaaaa agacaacctg caagcaattt caaagaacca
     5281 aaaattcaag ttttttatgg acaaccatca gagttttttt aaaaaaatca caggcccaat
     5341 acgtttttca tattgtatag gtccggtttt ttaccaataa cccattttgc cgcccgcaat
     5401 gcgccgcgaa caaacgtatc gcgggtatga gccgaatggc tgagtgttat cgtctcgccc
     5461 agattgccga tgtgtactga atgttcgccg acggtatcgc ccactcgcaa tgcatgaatg
     5521 ccgatttcgc cggtttttct cggacagacg ccgctgcggc cccgtacggc gacatcgttg
     5581 agttccacgc cgcgtgcgtc gcaggcagcc tcggcgagca ttatcgccgt tccgctgggg
     5641 gcatccttct tgaagcgatg atgcgtttcg acgatttcaa cgtcgtaatc ttcgccgagc
     5701 gttttgacgg cttgttcgac cagagtcgta agcaggttta ttcccatcga ataattgccg
     5761 gcgaaaacta ttggaatttc cttcgccgcg tcagccagtt ctgccttctg gctatcggtc
     5821 aggccggtcg tgccgatgac catggcaacg ccttttttgc ggaattgcgg cagccagtgc
     5881 atcgtgccag ccggcagcga aaaatctatc gccacatctg gaaaatctat gccgccgagc
     5941 gtgtcagcca ctttcagccc gaatctgccg acgcctgcca attcgccgac atctttcccc
     6001 aggtcgtcat ggccgctcat ttccagcgcg ccgaccacgt cgaattcgcc gctttctata
     6061 gccagcgccg ctatgcgtcg tcccattctg ccgccggctc cgattatcgc gagtttaatc
     6121 atattcattc tccaagcgtt tcaattatga accgccgcac agcctgcctg tcaccaggca
     6181 gtttcacgca gcgggtttcc atatccatga gcttttctat ccgtggatgc gtcgccagcg
     6241 aagcatcgcc cgtagcctcc gcaatagcag ccggaaattt tgccggatgc gccgtagcca
     6301 gacaaatcac cggctcgccg ccaatgccag acttttcagc cgccaccacg ccgcaggctg
     6361 tatgcgggtc gagcaaataa ccgtatcttt caaaatgcct gcgaatcacc tcaatgactt
     6421 ggccacagtt agccgatgac gatatgaaat cgccatcgat tccgccatca gccggcaaaa
     6481 cgctcaacga acctttttgg gcaaattcat ccatgatgtt cttcagcttt acggcatcac
     6541 tgccgaggcg atagtaaaga tatcgctcaa aattgctggc cacctggatg tccatgctcg
     6601 gtgagagggt ctggaaaact ttgccgatgg aatatcggcc ggtgttgaag aatcgggcga
     6661 gaatgtcgtt ttcgttagtc gccagaataa gtttgctgac aggcaggccc attttcactg
     6721 cataccagcc ggcgagcaca tcgccgaaat tgccggtcgg cacggcaatg cggacagttt
     6781 tcgctccggt agcccgctga acgtcgaaca cgccgctgaa atagtacact acctgcgcca
     6841 acacgcgagc ccagtttacg ctgtttaccg caccgagcga atatttacgt ttaaaatcca
     6901 ggtcagccga gagcgttttc atgatattct ggcaatcgtc aaaactgccg tcaacggcga
     6961 tattgtgaac gttgccgtcc aggacgctgg tcatctgtcg ttcctggata ggtgaaaccc
     7021 ggccggccgg gtgcatgata aagatattta tgccagcctt gccgcgcacg ccgtatatgg
     7081 ctgcactgcc ggtatcgccg ctggtcgcac caagaatgtt cagccgcccg ccgcgatggg
     7141 ctaaaacata ttcaaacaga ttgccgagta attgcaaagc tacgtctttg aatgcaagcg
     7201 tcggcccatg ccagagctcc tggataaaga cgtccccgac cggcacaacc ggcgcaacac
     7261 cgccttcgaa tgcctgagga ttgtaggtgc gggtgacgat gtcatgaaga tctttttgag
     7321 ggatgtcggt agcgaacaga tgcaaaattt catacgcaag ttccggataa ctcagccgcg
     7381 caagctcagc cagccgccct gctacacatg gtatcgattc cggaatgacc agtccgccgt
     7441 cgtcggcaag gcccatcatc acggcatcct gaaattctat cggcgatatc ccgccgcgtg
     7501 tggaaatata tttcatcttc gattttcctg atatgaaatc cgcctcagtc aaacggacag
     7561 ttgtacatta acgcatttga ctcgtacagg acaaggataa ttgtcaaatt ttacccaccg
     7621 tcaaggctaa aactgggtca cgaaacgagc ttgataatgg ataaatccat atgtcaaaac
     7681 gcgcttgaaa ggattcgctt cgcggcgcct tcggcgtcga agcctttggg gcatccgctt
     7741 ggccaacggc cggcgcgtct gcaaaggttc gaatccggtt taattgaaaa ataacctgca
     7801 aatacccctc aatgcagagg taacatttat tatcgaacgc gcttgaaagg attcgaacct
     7861 ttgacctact gcttagaagg cagttgctct atccagctga gctacaagcg cccatatgtc
     7921 gagggccgca ttttacttat cgcgacatgt ttttacaagc catttggccg cctaatttga
     7981 taaatcgtac aaaagtttgt ccagcctgcg acggcaacct tgaagccagt cgataaacgg
     8041 gctaaaatgt ccgttccgat gcggcaaatt ccgcagtata tttgacttga atccggcaga
     8101 tgagtaagga tattatactt tgccgcattt tgtctaaaac gaatcgcaat aaaacgacgc
     8161 ttttataaac tttaaaacaa accgaaagga cggaattgat gtcagacatc gataaagtat
     8221 tcaaggctta cgacgtgcgg ggaatatacg ctccggggga aatagacgaa gacctggcat
     8281 ggaaaatcgg ccacgctacc gcacagtttc tacacaaaag catgagcgga tacgaccgcg
     8341 gccaggcgtc ggttaacaaa atcgtcgtcg ggtacgacat gcggccgcac agcattccgc
     8401 tggcgacggc tctcatggaa ggcatcgcat caagcggcgc gacgtgcata aatatcggca
     8461 tgtgcgatac gccgatggtc tatttcgcca taaatcatct tgaggcatgc ggcggcataa
     8521 tggtgacggc ttcacataac ccgatagagt ataacgggtt taaaatctcc agctacaagg
     8581 ccaaacccgt cggcagcgca actggcctaa acgacatccg cgacatgtgc aaaaacattc
     8641 agcgtacgcc agccccttcg gaggcgacgc ctgtgcagaa cgtcgacctc tgggccgact
     8701 atcgtaaaca tgtgcttaaa ttcctgaatc tgccgcgcaa actcaaggtc gtcgtggatg
     8761 catcaaacgg catgggcggc cggatgatac cggccatatt cggcggcgta aaaaaccttg
     8821 aactcgtcga gatgaatttc gagatgggca aaggtttcgt gcatcagccg aatccgctgg
     8881 tggctgcaaa catgaaatgg gtacaggacg gcgtcaaagc caacaaagcc gacttgggca
     8941 tatgcctgga cggcgatgcc gaccgctgta tgttcgtcga cgaaaaaggc tgcataatcc
     9001 gctgcgacat aatgactacg tggctggcga tggatttcct gaaagacaac cccggcgcca
     9061 cgatagtata cgacctgcgc agctcaaaga ttcttccgga aaaagttcgt gcctgcggcg
     9121 gcgtgccgcg acgtgagcgg gtcggccacg ctttcatgaa aaaagccatg gccgatactc
     9181 aagccgtatt cggtggagaa ctgtccggcc acagctactt ccgcgataat ttttatgcgg
     9241 attccggcgc gattgtcttc gccagagcca tttcagttct ctcggcgtct gacaaaccca
     9301 tcagcacaca ggttgccagc ctgtgcaaat attctcactc aggcgaaata aacttcgaag
     9361 tcgaggacaa ggaaggcaag atgaaggaaa tcgcccaaaa attccccgac gcagaaattg
     9421 attacctcga cggcgtaacc tgcacgtatc cgaactggtg gtgcaatgtg cggcccagca
     9481 acaccgagcc gctgctgcgg ttaagcctcg aagccgaaac gcctgcactg atgaaagaaa
     9541 aactggcaat tatcgaaaaa atgctcggca aaccggtaga ccattaatta taaacctttg
     9601 ggaaaaatac tgcggcctgg caagccggcg tttaaatttc gccgacacat atgccaagct
     9661 tttcaggaga atttttatat gaaaaacata atcggacttt tcgtactggg acttatcgtc
     9721 gccggctgcg acaaggaagt gaaattgacg ttcgtcaatg aaacacgtaa cgacctgccg
     9781 ctgtccctca aaacttccag cggcattaac gaatcgctcg gcatcatgcg cgccgaaggc
     9841 agccatgccg aacgaacgtt caaatataaa aaagatgaac tgcccgtcaa cgtgacatgg
     9901 aaagccggcg aatactgcgg caaatttgac attaccgaca aaacgaacaa aaacctgaga
     9961 atcgactttc tgccggacgg accgt
//
LOCUS       DAONFI010000041         9927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7754_length_9927_cov_4.145158, whole genome shotgun sequence.
ACCESSION   DAONFI010000041 DAONFI010000000
VERSION     DAONFI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7754_length_9927_cov_4.145158"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1386
                     /gene="ribD"
                     /locus_tag="PLK08_02285"
     CDS             298..1386
                     /gene="ribD"
                     /locus_tag="PLK08_02285"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPS52356.1"
                     /translation="MTNCEQFMAIALDLAVRGRGEVEPNPMVGAVVVRDGRIIGRGWH
                     RKFGQAHAEINAIADAGYDVAGADVYVTLEPCSHYGKTPPCADALITAGVKRVFVSVA
                     DPDERVAGRGIQKLRDAGIEVVVGVSEQAGRELLKEYLKLRTVHRPWVICKWAQTLDG
                     RIDLGRHSEHRWISCEASRRDVHILRSFCDGILVGVETVVKDNPMLTNRSGEGRQPVR
                     VVLDGSLRIPDNCKLVKTAREFSTLVVTQGIFGDSQKAAALRDAGVEVITLPADAGRI
                     NLPSLLDVLGRRDWTRLLVEGGATVHGAFIDSRLADEAQAYIAPFNAPAEYDLPLFPA
                     DNLPPCYQLHSEQQIGVDTFKTYRLVSC"
     gene            complement(1460..1816)
                     /locus_tag="PLK08_02290"
     CDS             complement(1460..1816)
                     /locus_tag="PLK08_02290"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HPS52357.1"
                     /translation="MIKILERSGGNVIGYQAIGKITEEDMDTCIKYTDEIIKKYSRIS
                     WLYIMTDFKGGTWRAIYEDMIWLFKNLKHFDRMAVVGNKKWHELMIKADGLIFGEKYF
                     DISLLEEAWKYVEGKE"
     gene            complement(2080..2478)
                     /locus_tag="PLK08_02295"
     CDS             complement(2080..2478)
                     /locus_tag="PLK08_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPS52358.1"
                     /translation="MTEKNDKLQVNAEPAKVQPDNDSYMPLCDIYEEADGTNIMVAEI
                     PGAKSGTVDIRVDKGVLTISADGTRQAPGEDFNRTYGGFAGGHYFRAFALSDDVDRDS
                     ISATFKDGLLTLRLPKAPSAKTRKIPITHE"
     gene            complement(2499..2942)
                     /locus_tag="PLK08_02300"
     CDS             complement(2499..2942)
                     /locus_tag="PLK08_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPS52359.1"
                     /translation="MTKITLAGRWDPFAGLRAMQRELERFTSRGFFGEAPSLDAGMFP
                     PINVWSSPKEIVVECELAGIRRDDIELSITGDTLVIKGNKKPSTDDENVRYHRAERGA
                     GEFTRTIVLPESVDADKISANMAEGVLTVRMPKAESAVPRKIAVK"
     gene            3113..4210
                     /gene="mnmA"
                     /locus_tag="PLK08_02305"
     CDS             3113..4210
                     /gene="mnmA"
                     /locus_tag="PLK08_02305"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPS52360.1"
                     /translation="MPLDIIWLADMLLSAMPEKILLAMSGGVDSSVAAVLLQRAGYEV
                     TGAFMRISSNGEVGCSSRRDADDVRRVAGRLGIELHELDMAENFQEIISYFVSEYAAG
                     RTPNPCVMCNRKFKFGRLAKLADSLGITRLATGHYALRTIYKDGRPVIARGANLKKDQ
                     SYVLFAINADVIGRIVLPIGEQADKTLVRRMAEEIGLDVHDKPDSQEICFVQNDDYVQ
                     LVRQLAPQAMKSGRIIDSAGNELGRHDGYVRYTIGQRRGLRVAAGVPMYVIKIDPESG
                     DVTIGTRDESMSRSLTAKNLNWHIQPAAERFRANVQIRYNHRGAPATVTLTGDDSFHV
                     EFDEGIHAITPGQAAVVYDGDFMLGGGFICS"
     gene            5183..5569
                     /locus_tag="PLK08_02310"
     CDS             5183..5569
                     /locus_tag="PLK08_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS52361.1"
                     /translation="MTRTQKNQRNQLKIVVYISAGFACLMLAVSLALFLGNTEEPVAV
                     NTQNAQTGKVYDLEGTDVDKVEIPVSKIKPLEWPVNGNLLKAGKKALAFGVDESQDAR
                     VPEPATLALLAVGGLGIMTHYRRKRG"
     gene            5743..6153
                     /locus_tag="PLK08_02315"
     CDS             5743..6153
                     /locus_tag="PLK08_02315"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS52362.1"
                     /translation="MSIKRNKKSSAKMVLISSAAVVISLFAIAVCLFANGGATPCIQT
                     QKMDFGPAYKLEDLDSVDGMVMAKTDAVDLYKNIGTQNSIDLKGLDESLLKGQVRGIE
                     GLDETQVPEPATVTLLALGGLAMLRKRRGSRKNA"
     assembly_gap    6571..6670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6904..8226
                     /gene="thiC"
                     /locus_tag="PLK08_02320"
     CDS             6904..8226
                     /gene="thiC"
                     /locus_tag="PLK08_02320"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPS52363.1"
                     /translation="MKTQYEQAKSGQITPQIRRVAERECVDAELVRSELAAGRLVIPA
                     NVRHMAGTGDGHGVKLDPCGIGRAVTTKINANIGASPISSCKNEELLKLQWAVKYGAD
                     AVMDLSTGGNLDDTREHLLANSSVPVGTVPIYSMIVNKSVDELNRDDILKTVEKQAAQ
                     GVDFFTIHAGFRKAHIPLAMKRKLGICSRGGSLLAKWMTHHNAENPMYELFDDISAIM
                     HEYDVAYSLGDGLRPGCLADASDAAQFAELDTLGELVQRAREAGVQVMVEGPGHVPFN
                     EIQMNMERQQKICDGAPFYVLGPVVTDVFPGYDHITSAIGATAAGTYGASFLCYVTPT
                     EHLGLPRPADVKTGCIAYKIAAHAADIARGLPGARKWDDAMSDARAEFNWERQFELAF
                     DGETAREIRNRDADKQAGDEHYCSMCGKDWCAVRISRELAEKKQFENY"
     gene            8441..9658
                     /locus_tag="PLK08_02325"
     CDS             8441..9658
                     /locus_tag="PLK08_02325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPS52364.1"
                     /translation="MSPEVFIQKYGFFQLLEKYWYFGLVAFVVSLILTPVARSFAYKF
                     RIVDKPDDLLKPHCRPVAYLGGIGIAIGLLAGLADYGVYFWRSTPDKVEYFKNSLMHL
                     DIAALQTSRLWMLIMIAVACIIITLVGLVDDIRNITPRAKIFGQMLAAGFLLLGGVGL
                     KLFLTLFGLCGCCFPAWLVVVGSCIICMVLVICTCNATNLLDGLDGLCGGVTAIIALG
                     FLCLATWLATWGRFPATDELRIAITLAMAGAILGFLPYNIPPASIFMGDAGSMLLGFF
                     VAVMISLFSSEGLARWAMAACVVYALPILDTSLAVVRRVIAGRKIFEGDRGHLYDQLV
                     DRGMSVKKVVLLFYVLSVLAAVIGNVVAIKIRFRYALVIYSLLFLTVWVVFVYKGMVN
                     PDEKRKDAKKDIA"
BASE COUNT         2372 a         2276 c         2662 g         2517 t
ORIGIN      
        1 agcctaccca gccgtgacaa ggcggagaat ggtaaggctt tatgcccata acgtcaagtt
       61 taatggcgtc aagccacaag attttatcca aaaaaacggc tatacgcaaa attttttttg
      121 attatggcga tttcttttgc gttaagctat aatcctgcgg aaggtgggat tgcttctcag
      181 ggtgattcat atggatgagt attgcaggca gcttgcgtta gaatcatgtt ctgccggtta
      241 cttatagctg ttattgaata ttgcagctga ctcaatcctg aaaaattttt aagcgaaatg
      301 acaaattgcg aacaattcat ggcgattgcc cttgatttgg cagttcgggg caggggagag
      361 gttgagccca atccgatggt tggcgcggtt gtcgtgcgcg acggccgaat aatcggccgt
      421 ggctggcaca ggaagtttgg ccaggcacat gcggaaatca atgccatcgc cgacgcaggt
      481 tatgacgtcg ccggcgccga tgtttacgta acgcttgagc cttgcagtca ttacggtaaa
      541 actccgccgt gtgccgacgc cctgataacc gccggagtaa aacgggtttt cgtttctgtg
      601 gcagatcctg acgagagggt tgccggcagg ggcattcaga agctgcgaga tgcgggtatt
      661 gaggttgttg tcggcgtcag cgaacaagcc ggccgtgaat tgctgaagga atatcttaaa
      721 ttgcggactg ttcatcggcc ctgggtcata tgcaaatggg cccagacgct cgacggccgt
      781 atcgacctgg gccgccacag cgagcatcgc tggatatcgt gcgaggcatc ccgccgtgac
      841 gttcacattc ttcgcagttt ttgtgacggc atacttgtgg gcgttgagac ggtcgttaag
      901 gacaatccca tgttgaccaa ccgttccggc gaaggccgtc agcctgtgcg cgtcgtgttg
      961 gacggcagcc ttcgaattcc ggacaactgc aagctggtaa aaactgcacg cgaattttcc
     1021 acgctggttg tgacgcaggg aattttcggc gattcgcaga aagctgccgc tctccgcgat
     1081 gccggcgttg aagtcataac tctgcccgcc gacgccggca gaatcaatct gccttcactg
     1141 ctcgatgtgc ttggccgccg ggactggact cgtctgctgg tggagggcgg tgcaacggtt
     1201 cacggtgcgt ttattgactc acggcttgcc gacgaagcgc aagcctacat cgcgccgttc
     1261 aatgcgccgg cagagtatga tttgcccctt tttccggctg acaaccttcc gccgtgctat
     1321 caacttcatt ccgaacagca aatcggcgtc gatacgttca aaacgtacag attggttagt
     1381 tgttaaagct gaccgatttt aggaatcggt tttatttcgt ataggtcgcg gtgcggcgtt
     1441 ccactgcctc gtcaaaaatt cactctttgc cttcgacgta tttccatgct tcttccagta
     1501 gtgatatgtc gaaatatttt tcgccgaaaa tcaggccgtc agcctttatc atgagttcat
     1561 gccatttttt attgcccact acagccatcc tgtcgaagtg cttgagattt ttgaacagcc
     1621 aaatcatgtc ctcgtagatg gctcgccatg tgccgccctt gaaatcggtc attatgtaca
     1681 gccagcttat cctgctgtat tttttaatga tttcatccgt atacttgatg caggtatcca
     1741 tgtcttcttc ggtgattttg ccgattgcct gatagccgat gacgttgccg ccgctgcgtt
     1801 ccagaatttt aatcattttt ggactccttg tattgggtgg cctttcttta tgaaattata
     1861 ggcatttatt aaaggtatat tctaaaaaaa ccgtttcctg ttttttgaat atttttgggc
     1921 gattggcaga atcggtctgc cggcgagatt tgcgcaaaac gaaccccgcc gaaaaatttt
     1981 ggagcatttc aaattttaca tgaccccata ttatggggat ggctctgaaa aattcctgcg
     2041 gggcgcgttc aatggatttc cgtcaaccgt cgacggagac tattcgtgtg tgattggtat
     2101 ttttctggtt tttgcggatg gcgccttggg cagccgtagc gtaagcaggc cgtccttgaa
     2161 cgtggcggag atgctgtccc tgtccacatc gtcgctgagt gcgaacgccc tgaaataatg
     2221 gccgcctgcg aagccgccgt aagtcctgtt aaaatcctcg cccggagcct gccgcgttcc
     2281 gtcggctgat atcgtcagca cgcccttgtc gacgcggata tcgaccgtgc cggattttgc
     2341 gcctggtatt tcggcgacca ttatgttcgt gccgtcggct tcttcgtaga tgtcgcacag
     2401 cggcatatag gagtcattgt ccggctgaac tttcgcgggc tctgcgttta cctgtaattt
     2461 atcatttttt tcagtcattt tcgttagcct ccttttgcct acttgacggc gattttgcgg
     2521 ggcactgccg attcagcctt gggcatgcgg acggtcaata cgccctcagc catgttggct
     2581 gatatcttgt cggcgtccac ggattcgggc agaactatcg tcctggtgaa ttcgcctgcg
     2641 ccgcgttcag ccctgtggta gcgtacattt tcgtcgtcgg tcgagggttt tttattgccc
     2701 ttgattacga gcgtgtcgcc ggttatcgac agttcgatgt cgtcgcgtct tatgccggct
     2761 agctcgcact ccacgacaat ttccttcggc gacgaccata cgttgattgg cgggaacatt
     2821 ccggcgtcga ggcttggcgc ttcgccgaaa aatcccctgc tcgtgaatcg ttcgagttcc
     2881 ctttgcattg cacgaaggcc ggcaaagggg tcccatcttc ctgccagtgt tattttcgtc
     2941 atttttctct cctttccggc gacagacact tttcgccgtt gccctggtaa taagcaaata
     3001 cgatgccagt tatgtaactt attttttatc agagtgttaa cgtttgtgtg ttttttgagg
     3061 aaaatgtcaa aatggccgcc gtcgtcgaac gtgtcgccaa aatgacattt ttatgccgct
     3121 tgacattata tggctggcgg atatgctttt gtccgcaatg ccggaaaaaa tattgctagc
     3181 gatgagcggc ggggtggatt catccgtggc ggcggtgctg ttgcagcggg caggctatga
     3241 ggtgacgggg gcgtttatgc gcataagttc aaatggcgaa gtgggttgct cgtcccgtcg
     3301 cgatgccgac gatgtccgcc gcgtagccgg ccgactggga atcgaactgc acgaactgga
     3361 tatggctgaa aattttcagg aaataatctc gtatttcgtc tcggaatacg ccgccggccg
     3421 aacgcctaat ccgtgcgtga tgtgcaatag gaaattcaaa ttcggcagac tcgcaaagct
     3481 ggccgattct ctgggtataa cccgcctggc taccggccat tatgcgcttc gaactatata
     3541 taaggatggc cggccagtca tagcccgcgg tgcaaatctc aaaaaagatc agtcctatgt
     3601 gctctttgcc ataaatgccg acgtcattgg ccgcatagtt ctgcctatcg gcgagcaggc
     3661 tgacaagacg ctggtgcggc gaatggctga agaaatcggc ctggatgtgc acgacaagcc
     3721 ggacagccag gaaatatgtt tcgtacagaa tgacgattat gtacagctgg ttcggcagct
     3781 tgcgccgcaa gcgatgaaat ccggcagaat catcgattcc gccggcaacg aactgggcag
     3841 acatgacgga tatgtccgtt acaccatagg ccaacggcgc ggccttcgcg tggcagccgg
     3901 cgtacccatg tacgtcatca aaatcgaccc tgaatcaggc gatgtgacca ttggcacgcg
     3961 ggatgaatcc atgtcgcgca gccttacggc gaagaatctc aactggcata tccagccggc
     4021 ggcagaacgg ttcagggcaa acgtacagat acgctacaat catcgcggcg ctccggcgac
     4081 ggtgacgctg accggcgatg attcttttca tgttgaattc gacgaaggca tacacgcaat
     4141 aacccccggc caggcagctg tcgtttacga cggcgatttc atgctcggcg gcggattcat
     4201 atgctcgtaa tccgttgtcg gcagctttat tagagaacca ctctattagg atacttcatt
     4261 tgtttcagag aagatttttg cgagccgcgg atatcgtata ttgatataaa gtctttatga
     4321 gaaataagtt atagactgta aaataatttt gagaaaatta caaaaagact tgcaaatgaa
     4381 ttgtgaaagt gtaagattat tcatggcggt tgagccccgc cagattgtat tagatagccc
     4441 tgataattaa aggctggtac aatattcagg aatgagagaa tcctgaaaat atgttttgta
     4501 aaaaattgca aaaatataaa aaattgtact tgacctcgaa atcgggacag ctataattaa
     4561 gcatgagcct gaagaactag gacgggattt gcgagccgag agagagaagg cacgccttaa
     4621 acctcaatcc aattcttcaa tgttatttta cattttgtgt ttttattcaa tttgcagtac
     4681 ggagctgagc tggctctgcc gttgttagag agaacgacgg gaattgaaat aaagcacacc
     4741 ggtcagcgat gctgattgaa gagcctgaat ctttttggaa ggtaaattgt aagtttcatt
     4801 gtatttttgc atttcagcgc ttcttcaaat tttgccggct gatgttttac gcagtttgtc
     4861 cgaatgagcc cggatattaa acatcacgaa atgtgccagc cattttatgg ctaccttata
     4921 acgagagaga gaaaacccga ttgacgaaag tcaaagagga gccttaacgt cagggacagt
     4981 gtatccccgc acacctcacg agttttctac ggtcgcttga tgcatggcat gatgtgccgc
     5041 ccataggagc agagagcgaa ataatcttaa atgtttatat gtccatgagt gagttgcttg
     5101 tgggaatttg ctcaagaagg atacgagcgg ccggacgtca tgtccggcgg agacgagagc
     5161 ctagattttt aggagagaga aaatgacgag aactcagaaa aatcagagaa accagctgaa
     5221 gattgtagtg tatatctcgg cggggtttgc atgcctgatg ctggcggtat cgctggcgct
     5281 gtttctgggc aacaccgaag agcccgttgc cgtgaatacg cagaatgcgc agacgggcaa
     5341 ggtttacgac ctggaaggga cagacgtcga caaggttgaa attcctgtca gcaaaatcaa
     5401 gccgcttgaa tggcctgtca acggcaatct gttgaaagcc ggcaaaaagg ccctggcctt
     5461 tggcgtcgat gaatcgcagg acgcccgtgt gcctgaacct gcgacgctgg ctctgctggc
     5521 ggttggcgga ctgggaataa tgacccacta tcgccgcaaa cgcggttgag atgagaacga
     5581 actgcttggg ctgccctgag agaacagccc gaacagtgga tgacaaaggt tgtctgtgac
     5641 ggcgaatgac gagagagaaa atcgccggac tgacaaagga cgagagagtt acttaaagag
     5701 cggaacgacg gcccgcaaag cccgtcgaac aggagcctga ccatgagtat caagagaaac
     5761 aaaaaaagtt ctgccaagat ggttttaatt tcaagtgcag cagtagttat ttcccttttt
     5821 gcgattgcag tatgtctttt tgccaacggc ggtgcgacgc cgtgcattca gacgcagaag
     5881 atggatttcg gcccggcgta taaacttgag gatttggatt ctgtagacgg catggtgatg
     5941 gccaagactg acgccgttga tctttacaaa aacatcggga ctcagaattc gatagacctc
     6001 aaagggctgg atgagtcgct tcttaagggt caggttcgtg gaattgaggg tttggatgag
     6061 acccaagtgc ctgaaccggc taccgtgacg ttgctggccc tcggcggcct ggcgatgctt
     6121 cgcaaacgtc gtggaagcag gaagaacgct taaataattc gcccgaaggt tgcaacaact
     6181 gccggctgat agggtcggct gatagacgaa cttaaaactg aatagaactt gtgagcagcg
     6241 tacgaaacgc aagccggctg gcgacttata tggtgcatgg cgccgtcgga tacacgagag
     6301 ataaacggcc gggaggcctg tgattgcagg tctccctgtg ccgaaattcc gaaacgaaat
     6361 tctctctctc gtcaggctcc aaggctgggg tggcccgcag agagctgggg ccaccttggc
     6421 cgcctgtttt ttaaaaaaag acaaaaaacg cagataacag atataaaaaa tctttctgac
     6481 gtaaaacagg gcctgacgcg ggattaacgg gtggaaaacg aaattctctc tctcgtcagg
     6541 ctccaaggct ggggtggccc gcagagagct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn ggggccacct tggccgcctg tttttttgcc tgataagcaa tgaaatacat
     6721 gctgctccaa gttttttaat catgttaaaa aatcaggccc ggaatcgtcc gggctttttt
     6781 atgcgcatca aacgctgttg acggtggaaa atggatagtc gatttcgtat aataatatcc
     6841 ttttattgcg aggccgtagg gtagtcccgc ctttgcgccg cttgaaatga tgaaagaaca
     6901 aagatgaaaa cgcagtacga acaggcaaaa tcaggacaga taactcccca aattcgccgc
     6961 gtagctgagc gcgaatgtgt agatgcggaa cttgttcgca gtgaattggc tgcagggcgg
     7021 ttggtgatac cggctaacgt gcgtcatatg gctggcacgg gtgacggcca tggcgtaaag
     7081 ctggatccgt gcggcatcgg cagggcagtg acgaccaaga taaatgccaa tataggcgca
     7141 tcgccgataa gcagttgcaa aaacgaagaa ttgctcaagc tccaatgggc tgtgaaatac
     7201 ggtgccgacg cggttatgga tttgtccacc ggcggcaatc tggatgatac gcgggagcat
     7261 ctgctggcga attcatccgt gccggtaggg accgtgccga tatattccat gatagtcaac
     7321 aaaagcgtcg atgagcttaa ccgtgacgac atcttaaaga ccgtggaaaa gcaggctgcc
     7381 cagggggtgg acttttttac catccacgcc ggctttcgca aggcgcatat tccgctggca
     7441 atgaagcgta aattgggaat ctgttcgcga ggcggctctc tgctggcgaa gtggatgacg
     7501 catcacaatg ccgaaaatcc catgtatgag ctgtttgacg atatttccgc cataatgcac
     7561 gagtacgacg tggcatattc gcttggcgac ggcttacggc ccggctgcct ggctgatgcg
     7621 tcggacgctg cccagtttgc cgaacttgac acattgggcg aactcgtcca gcgtgcgcgt
     7681 gaagccggcg tacaggtcat ggtcgaaggc ccaggccatg tgccgttcaa cgaaattcag
     7741 atgaacatgg aacggcagca gaagatatgc gacggtgcgc cgttttacgt tctcggccct
     7801 gtagtgacgg acgtttttcc tggttacgac catatcacct cagccatagg tgcgacggct
     7861 gccggcactt atggcgccag tttcctctgc tatgttacgc cgacggaaca cttgggcctg
     7921 cctcggccgg cagacgtaaa aaccggctgc atagcatata aaatcgccgc ccatgccgcc
     7981 gatatcgccc gcggcttgcc tggagcaaga aaatgggacg acgccatgag cgatgcgaga
     8041 gctgaattca actgggaaag acagtttgag ctggcgttcg acggtgaaac cgcacgcgaa
     8101 ataagaaatc gcgatgctga caaacaggct ggcgacgaac attactgctc aatgtgcggc
     8161 aaagactggt gcgccgtgcg catcagccgt gagctggctg aaaaaaaaca attcgaaaat
     8221 tattaatccg catggtattc tataccatcg agtttgtcgg gtagatgcgt tttgccgccg
     8281 aaagcatatt gtgacaaaat gcgtgggata ggttctagct gcctttgata ctccttgcac
     8341 tggcgtgata agtttggtaa tctgtttcct tgtaataact gttgttattt aaagcagata
     8401 ggtaaaatag ccaagccatg tttgtcctgg agaataaata atgtcccctg aagtgtttat
     8461 acagaaatac ggtttttttc agcttttgga aaaatattgg tatttcggcc ttgtagcatt
     8521 cgtagtttcc cttattctga cgccggtcgc cagaagtttt gcttacaagt tcaggatagt
     8581 cgataaacct gacgacctac tcaagccgca ttgccggccg gtagcgtatc tgggtggaat
     8641 cggcattgcg ataggccttc tggctggcct tgccgattac ggcgtatatt tctggcgatc
     8701 gacgccggac aaggtggaat atttcaaaaa ttccctgatg cacttggata ttgcggcatt
     8761 acagaccagc cgtctgtgga tgctgataat gatagctgtt gcgtgcataa tcataacgct
     8821 ggtcggcctt gttgacgata tcaggaatat cactcctcgc gccaaaatat tcggccagat
     8881 gcttgccgcc ggttttctgc tgctcggcgg cgtaggactg aaactgtttt tgacgctgtt
     8941 cgggctatgc ggctgctgct ttccggcgtg gctggtggtt gtgggcagtt gcataatatg
     9001 catggtactg gtgatttgca catgtaacgc caccaatctg ctcgacggcc ttgacgggct
     9061 gtgcgggggt gttacggcga taatagcgtt gggctttctc tgccttgcaa cgtggttagc
     9121 cacctggggc cgattccctg cgactgatga actgcgaatc gccatcacat tggcgatggc
     9181 tggagccata cttggatttt tgccgtataa cattccgccg gccagcattt tcatgggcga
     9241 tgccggcagc atgcttttgg gttttttcgt cgcagtaatg atatcactgt tttcgagcga
     9301 aggccttgcc aggtgggcaa tggcagcctg cgttgtctac gccctgccga ttctggatac
     9361 gagtcttgcg gtggtacgcc gcgtgattgc aggaagaaaa atattcgaag gcgaccgcgg
     9421 ccatctgtac gaccagctgg tcgaccgtgg catgagcgta aaaaaggttg tacttttgtt
     9481 ttacgtttta tccgtacttg cggcggtgat aggaaacgtg gtagctataa aaataagatt
     9541 ccgctatgcg ctggtcattt actcgctgct gtttctgacc gtatgggttg tttttgtcta
     9601 taaaggtatg gtaaaccctg acgaaaaaag aaaagatgca aaaaaagata ttgcttaggg
     9661 aacattgttt atttattccc gtcaaacttt ttataaaggc agcaacaaga aaatctttgt
     9721 gctggacacc cgcattaaaa attggtaaaa atacaaaact tcggcgcaag tcgtttgaaa
     9781 aattgttaaa gaatgcgaat ttttgcgcaa aatcattttg aaaaattgtt aaagaatacg
     9841 aaattttgcg ccaagtcgtt ttaaaaaatt ggtaaagaat acgaaatttt gcgccaagtc
     9901 gttttaaaaa attggtaaag aatacga
//
LOCUS       DAONFI010000042         9780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7920_length_9780_cov_4.088535, whole genome shotgun sequence.
ACCESSION   DAONFI010000042 DAONFI010000000
VERSION     DAONFI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9780
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_9780_cov_4.088535"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..1532)
                     /locus_tag="PLK08_02330"
     CDS             complement(285..1532)
                     /locus_tag="PLK08_02330"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPS52365.1"
                     /translation="MSIVVMKFGGSSVADADKIRAAASRAIKTKKAGHNVVMVVSAMG
                     DTTDNLVALADEVNRGKPAPKREMDQLLVTGEQVTISLMAMALEEMGQPAISFTAGQI
                     GMITDDRHTKARLHTIDEKIIKRELAKGRIVIVAGFQGVTPSGDVTTLGRGGSNVTLV
                     AVAAALKADVCENFTDVDGIYTADPRKVPNARKIDYISYDEMLELASLGANVLHNRAV
                     EFAKKYNVPIHVRNSQNNNKGTMIVAETPSMERIVVSGAALKDKLARISLRHVPNIPG
                     QAAGIFHTLASNDIVVDDIIQTIGENTATVSFTVDLGDFDDAKIVCDKLAKKLNCQVV
                     YETDIAKVSVVGVGMRVHTGVAQKMFEALAKAKINIQNITTSEIKISCLIRRQQGAEA
                     LQLVHDAFNLGKKPQKKVKKTAR"
     gene            1866..2858
                     /gene="lpxK"
                     /locus_tag="PLK08_02335"
     CDS             1866..2858
                     /gene="lpxK"
                     /locus_tag="PLK08_02335"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPS52366.1"
                     /translation="MCRLFKIINRVVGWPYLAVMAVRRWAYGKGFLRSYSAAVPVICV
                     GNITTGGTGKTPMVAFVVKALQSAGRKPAIITRGYRAVDGKSDEAELLGAMTGADVIV
                     NPDRVAGAAEAVRRGADIIVMDDGYQHLRLRRDFNVVLIDASNPFGGDAGLPLGRLRE
                     CPRTLRFADAVVITRSDTIDELALDGLLGRIAAYGGDVPVARAVHSPVDLIDLNGDRK
                     SLKLLDGKRIFAFCGLGNPAAFERTLRQCGAEIAGMRLFGDHVHYTPAIAAELSRQAD
                     ACNAEMIVTTCKDGVKLSPEWFFKGIELFQLAIEMRLVGGEEFISRVESLGLRR"
     gene            complement(2821..3879)
                     /locus_tag="PLK08_02340"
     CDS             complement(2821..3879)
                     /locus_tag="PLK08_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS52367.1"
                     /translation="MENVNAHTHGKRTRIIVAIFCLAAAASIIYLCWPSAQQSLPMPP
                     APEPMDAEKVLLQAKELSGTGNINQAIALLQQYTFEHAGEITVSPTLAKLYILTGQHA
                     KASELMATLLKAQPDNPKILWLRGELEKTSGKDPMPWFRQAAELPAADNEIHTQYALM
                     LWDAKQYKPAEAYIKKALAGGSQDARIHQLAGHIALKEGRLEDAKTLLVYATEKDKQS
                     AQAWADLAETCKKLGQDKQAIAALKHVLQYARGDMRKNVLFELAGMLVEQKEYMQAAD
                     TYVKAADAQPTLKRNCLLEAAKCYWRIGKYALAMKYIDIVYELQPDDQEVTAWRTKIE
                     NARFGPVSGEAPKTQPAK"
     gene            4155..4991
                     /locus_tag="PLK08_02345"
     CDS             4155..4991
                     /locus_tag="PLK08_02345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPS52368.1"
                     /translation="MIERLNMLLPGERLLYAIMIGGGILLPFAIFKWVLNPEYYRRFR
                     NRPNTFTPFDFILLLLVWLTMNFILGRLAAMAAEGDRVTVKLIVILSVQLAIVPLELY
                     ICKYRFAGGLGRGGLGLMWKNLFSDVILAAACIIVVLPVCNVLAGAVTYWLRQFPDFS
                     LHEHELLRSLGSVGTGGKILIIVSATVGAPVFEELFFRGILQSMLRRALSPWPAILIT
                     GIFFAVVHPEIQNWPSLMILAASLGWTYEKTGRIMLPILIHAMFNAVYIAAYLLGVPK
                     VL"
     gene            complement(4986..7067)
                     /locus_tag="PLK08_02350"
     CDS             complement(4986..7067)
                     /locus_tag="PLK08_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPS52369.1"
                     /translation="MTQHNINKPTPELLAPAGDKESLVAAVQAGANAVYLGIRSLNAR
                     RGAVNFSHEELTRVVKYAHDNGVKVFLAVNTDIAAREVGQAVRALEVARIAKVDAVLF
                     TDPLFLAVRKFYPEVAFHFSTQGAIENSAGVEAAGELGADRVVLARELSKEEINAASG
                     KGVETEIFVQGALCFCVSGRCLLSSWGGGRSGNRGTCTSPCRAAWRLNGDGTPKRMLS
                     MHDLSLLEKLDEIRDAGVTCLKIEGRLKKADWVAKAVSIFRHALDEGATTSLKTTARE
                     LGDYTGRSLTSGYFDASRENLCGESGRPKTSTTIDGNSPKSEKSQDKSTNEYRLSIST
                     ASGRAECTLQSADNVENWDCPLSHVHNEKRSTTLKAVGEYLAELKVQDFTLADFIVDA
                     PALPIARKTANAIIDRVTTTLNRFAKKSQDTTIRVDLPANVRQAVAFNPPSAANNRPL
                     GAPPQAARISFDVYEEFKTAARDVHKLTIEHVPADRLNRLMKSEIPAMPAVFFESALP
                     AMRNWLARCAELDLKVEVNSWAGWRLAKETGVRFEGGPGMMVLNPVAARELATLGFEA
                     VTFSLEADRRQLEDFSANAETPYSLLVFGRPVLGYTRSKLPPEIKTGDIIEDARDIRL
                     QITQDGELTVFRPTSPFNFAHVKNRQIRAAWLVADMVSSPNAAEEWKMLQAGQSADKK
                     TFEFNYNRRLL"
     gene            complement(7067..8149)
                     /locus_tag="PLK08_02355"
     CDS             complement(7067..8149)
                     /locus_tag="PLK08_02355"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPS52370.1"
                     /translation="MPDARPELIFVAGPQKGQRTVLMDNNIIAGRSSQADIKITEETV
                     SRQHLRFALTIDGWIVENLSANKIEISGQSYKRGKQVLLETGDTISLGSETTLLFVSS
                     GDDPIAAMEKYLNTNTNQTQTPPPAAPEQAQQTPPAQKKSVKPESSSLKKSPADKLTT
                     TEKKRKTKLYVSAAIVYLIAMAGLFTFLALAKKSSSTVGNSEKPAILTRETIAAALTC
                     DLNHAPNAEASAKHLRLAREYYRTRNATDKNLYLCVKNYRLYLSFRRPDQKTFQIEDE
                     SQYNTAKKELIDKIMSIYENGWALENASHFSQAIGEFELLLQRYLPPSEMPQFNTVTP
                     EANDAMVSENIQDHISYISKFMRKKK"
     gene            complement(8149..9093)
                     /locus_tag="PLK08_02360"
     CDS             complement(8149..9093)
                     /locus_tag="PLK08_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPS52371.1"
                     /translation="MIFAEISETFWLGWSWDVALSAGLFLSVSMFVLAVFGQQGEIKL
                     SPQRAAAIATGHTDRKTLFENVLFKPFLWIFLAISYRLRSESLKRYISKQLTASGNPN
                     YYTPQEFLALSFMTGFGLGMVLELLHILVAGSPSISVIILGIAVGSSLCIIQLADQSA
                     KRMRSITRQLPYALDLISLAMGAGANFTEAVRTIVDEDPQDSEPLNVELRAMLAEIEL
                     GTTRQQALANMAQRVPLDSIQSLASAVTQAQQLGTPLANVLHDQASALRLRRSVRAEN
                     LAATASVRILVPCLLLVMAVILTIFGPAIIRIMRGGLF"
     gene            complement(9201..>9780)
                     /locus_tag="PLK08_02365"
     CDS             complement(9201..>9780)
                     /locus_tag="PLK08_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPS52372.1"
                     /translation="LPGAIIRKLQKKRLDRLETQIVEGIQTLASGVRAGLNLVQAMEL
                     IARDAPAPISQEFSHLLSEYEYGTPLDEAMLSTAKRIGSADYRLLFTALHTHRQQGGD
                     LGDTLDRIADSIREIQRLNARVDSLTAQGRATARWLGAMPLVVMCIMYFIPGSNVEKL
                     FTDDIGKLILSAVFLMNLVGFLWIRKIMEIDI"
BASE COUNT         1965 a         2391 c         2732 g         2692 t
ORIGIN      
        1 cctggatttc gtctgtcgcg ccgatagcga tgcaatctat cgtcgcgcga aggatatgct
       61 tgaacgttcg gaatcggacg gcggttatgg acttggtact ggcaacagcg tgccggcgta
      121 tgtcccgatg cagaatttct tccagataat ccgggcgtgt attgaatgac ttttttgaaa
      181 ttgtgcgcat aaaaaagcgg aaacgtttta ccgttcaaaa catttccgct tttttacttt
      241 cttacagaat accacggttt tacccgcggg gtatctacgt atttttatct ggcggttttt
      301 ttgactttct tttgcggttt cttgccgagg ttgaaggcat cgtgtaccag ttgaagtgcc
      361 tctgctccct gttgccggcg gatgaggcat gaaatcttta tttcgcttgt agtgatgttc
      421 tgaatgttta ttttagcctt tgcgagagcc tcaaacattt tctgcgccac gcctgtatgc
      481 actctcatgc cgacgccaac aacactgact tttgcgatat ctgtttcata tacgacctgg
      541 cagttgagct ttttggccaa tttgtcgcag acgatttttg catcgtcgaa atcgcccagg
      601 tctaccgtga aagatacggt ggcggtgttt tcgccgatag tctggatgat gtcgtctacg
      661 acgatatcat tagatgccag cgtatggaaa atgcctgctg cctggccagg gatattgggt
      721 acgtgacgca gactgatgcg ggcgagtttg tctttcaatg ccgcaccgct gacgacgatt
      781 ctttccattg atggagtttc agccacgatc atggttcctt tattattgtt ttgagagttg
      841 cgaacgtgaa tcggcacgtt gtattttttt gcgaattcga cggctctgtt atgcagcacg
      901 tttgcgccga gcgaggccag ttcgagcatt tcgtcataac ttatgtagtc tattttgcgg
      961 gcgtttggca cttttcgcgg gtcggcggtg tatatgccgt cgacgtcggt gaagttttcg
     1021 cagacgtcgg ctttaagcgc ggcggctacg gcgacgagtg tgacgttact gccgccgcgg
     1081 cccagggtgg tcacgtcgcc ggagggcgtt acgccctgaa agccggcgac gattactatt
     1141 ctgcctttag ccagttcgcg ttttattatt ttctcgtcta tcgtatgaag gcgcgccttg
     1201 gtgtgcctgt cgtccgtaat catgccgatt tggccggcgg tgaagctgat agccggctgt
     1261 cccatctctt caagagccat cgccatcagc gaaatcgtca cctgctcgcc ggttacgagc
     1321 aactgatcca tttcgcgttt cggagcgggt ttgccgcgat taacctcgtc ggctagcgcc
     1381 acgagattat cggtggtgtc gcccattgcc gagacgacca tgacgacgtt atggccggct
     1441 ttttttgttt tgatagcccg gctggccgcg gcgcggattt tatccgcatc tgccacactt
     1501 gaaccgccga acttcattac tactatactc atgctctgtt tttctcctga aaaccgcgca
     1561 ttgcaggcgg atttttgact ttacgtcgca gtaaatgctg ccggtataat acgtactaaa
     1621 gccatcgcaa tgcacataca ccgctatatt gatagcgaat attccgataa aagcaacaat
     1681 ctaacctgta aaaatgcaaa aaaccttaaa aattggtttt atttgccgcc gccagcctat
     1741 tggtgtaggg ctgaaactcc atttgttcaa cgctcatttt ttaaacagca tgacagtttt
     1801 ggccgtattc ggtattgtcg gaattatggt tgttttgcgg agtgtttttt gttctgatat
     1861 tgacgatgtg tcgattattc aaaatcatta accgtgttgt cggctggccg tatcttgcgg
     1921 ttatggctgt gcgcagatgg gcatacggca agggtttttt acgcagttac tctgccgccg
     1981 tgccggttat ctgcgtgggc aatatcacta ccggcggcac gggtaaaacg ccgatggttg
     2041 cgtttgtcgt caaggcattg cagtcggctg gccgcaagcc tgcgattatc actcgcggtt
     2101 accgtgccgt cgacggtaaa agcgacgaag ccgaactgct tggcgccatg accggcgcgg
     2161 atgtaatcgt taatcccgac agggtagccg gggctgcgga agcggttcgt cgcggggctg
     2221 atattatagt tatggacgac gggtatcagc atctgcgtct tcggcgggat tttaacgtgg
     2281 ttctcataga tgcgagcaat ccgttcggcg gcgatgccgg cctgccgctg ggccgtctgc
     2341 gggagtgccc ccgtacgctc agatttgccg atgccgttgt aataacccga agcgatacga
     2401 ttgatgagtt ggcgttggac ggccttttgg gcagaattgc tgcatatggc ggcgatgttc
     2461 ctgtggcgag ggcggttcat tcgccggtgg acctgattga tttgaacggc gacagaaaat
     2521 cgctgaaatt actggatggg aaacggattt ttgcgttctg cgggcttggc aatccggctg
     2581 cgttcgaaag aacgttgcgg cagtgcggtg ccgagatagc cggcatgcgt ttattcggcg
     2641 accatgttca ttatacgcct gctattgccg ccgaactgtc ccgtcaggct gacgcctgca
     2701 atgccgaaat gattgtcacg acctgcaagg atggcgtgaa actcagcccc gaatggtttt
     2761 ttaagggcat cgaacttttc cagcttgcaa tcgagatgcg actggttggc ggcgaagaat
     2821 tcatttcgcg ggttgagtct ttggggcttc gccgctgacg gggccgaaac gggcgttttc
     2881 aattttagtc cgccatgccg taacttcctg gtcgtcgggc tgaagttcgt aaacaatgtc
     2941 gatgtatttc atcgccagtg catatttgcc gattcgccag taacatttgg cggcttccag
     3001 caggcagttg cgtttgagcg tcggctgggc gtcggcggct tttacgtagg tatcggcagc
     3061 ctgcatgtat tccttctgct caactaacat cccggcaagc tcgaaaagca cattctttct
     3121 catgtcgccc ctggcgtatt ggagtacgtg tttgagggct gcaatcgcct gtttgtcctg
     3181 tcccagtttt ttgcaggttt cagccaggtc agcccaggcc tgagccgatt gtttatcctt
     3241 ttcggtggcg taaaccagca gagttttcgc gtcttcaagc ctgccttcct tgagggctat
     3301 gtggccggca agctgatgta tgcgtgcgtc ctgagaaccg ccggcgaggg cctttttgat
     3361 atacgcttca gccggcttgt attgtttggc gtcccagagc atgagagcat attgcgtgtg
     3421 gatttcatta tcggcggctg gcagttcggc agcctgtctg aaccacggca tggggtcttt
     3481 gccggatgtt ttttccagct cgccgcgcag ccacagaatt ttgggattat ccggctgggc
     3541 tttgagaagc gttgccatga gttcggaggc tttggcgtgt tggccggtga gtatgtagag
     3601 tttggcgaga gttggggaga cggtgatttc gccggcgtgt tcaaaagtat attgctgaag
     3661 cagcgcgata gcctggttta tattgcccgt gccggacaac tccttagcct gcaacaggac
     3721 tttttctgca tccatcggtt caggtgccgg cggcattggt agcgattgct gtgccgacgg
     3781 ccagcataga taaattatcg aggctgctgc tgccaggcag aagattgcga cgattatgcg
     3841 ggtacgtttt ccgtgcgtat gagcgttgac gttttccatc tcagatatcc tttacggttt
     3901 tacgattctg gaacatccgg ccctcgccgt cgcaggcggg cagacaaaag ccttttatcc
     3961 ttatatataa aaactcctgc aagtgccggc ggttttttct ttgaccgctg atatgcagaa
     4021 ataataatat attcaatttg ccgttgaaag tgaactaaaa acggctggct ggaaatttga
     4081 cgtgacccca tcccccaaac atgggcattg ccgattattt tttcccaagg ctgggcggtt
     4141 acaggaatta tcgaatgatt gaacggttaa acatgttgtt gccaggtgaa cggctgctgt
     4201 atgccataat gattggcggc ggcatattac tgccgtttgc aatttttaaa tgggtgctga
     4261 atccggaata ttatcgacgt ttcaggaaca ggcccaatac ctttacgccg tttgatttta
     4321 tcctgctcct gctggtgtgg ctgacgatga atttcatcct ggggcgactg gcggcgatgg
     4381 ctgccgaagg cgatagggtg acggtcaaac ttatcgtcat actttccgtt caattggcta
     4441 tcgtgccgct ggaactttat atttgcaagt atcgctttgc cggcggactt ggtcgcggcg
     4501 ggctcggact gatgtggaaa aatctttttt ccgacgtgat tctggcggca gcctgcataa
     4561 tcgtagtttt gccggtatgc aatgttctgg ctggtgcggt gacgtattgg ctgaggcagt
     4621 ttccggattt ttcattgcat gaacatgaac tgctgcgcag ccttggcagc gtcggtacgg
     4681 gcggaaaaat tctgataatc gtttcggcaa cggttggtgc gccggttttt gaggagttgt
     4741 tttttcgcgg catattgcag tcgatgttac ggcgggcgtt gtcgccgtgg ccggcgatat
     4801 tgattactgg catattcttt gcggtggttc atccagagat tcaaaattgg ccgtcgctga
     4861 tgatattagc cgcctcgctt ggctggacat atgaaaaaac cggcagaatc atgctgccga
     4921 tactcattca cgccatgttt aatgccgtct acattgctgc gtacctgctt ggcgtaccga
     4981 aagttttata gcagtctgcg gttgtaattg aattcaaaag tttttttgtc ggctgactgt
     5041 ccggcctgca gcattttcca ttcttcagcc gcgttcggcg atgataccat atccgctacc
     5101 agccacgccg cgcgtatctg ccggtttttt acatgggcga agttgaacgg cgatgttggc
     5161 cgaaaaaccg tcagttcacc gtcctgcgtt atctggaggc ggatgtctct tgcgtcttcg
     5221 atgatatcgc cggttttgat ttcaggcggc agttttgacc tggtatagcc caggactgga
     5281 cggccgaaaa ccaacagtga atatggcgtt tcggcattgg cggaaaaatc ttcgagttgc
     5341 cggcggtcgg cttcgagtga aaaggtgacg gcttcaaaac cgagtgtcgc aagttcgcgt
     5401 gccgctaccg gattcagtac catcatgcct ggcccgcctt cgaatcgtac gcctgtttct
     5461 tttgccagcc gccagccggc ccacgagttg acttccactt tcaaatcgag ctctgcgcag
     5521 cgggcgagcc agttccgcat ggctggcagt gccgattcaa aaaataccgc cggcattgcc
     5581 ggaatctctg atttcatcag ccggttaagt ctgtcggcgg gtacgtgttc tatggtcaat
     5641 ttgtgtacgt cgcgcgcggc ggttttgaat tcttcgtaaa cgtcaaaaga tattcttgca
     5701 gcctgcggcg gagcgccgag cggcctgttg ttggcggctg acggtggatt gaaggctacc
     5761 gcctgccgaa catttgccgg caaatccacg cgtatcgtcg tgtcctgtga tttttttgca
     5821 aaacggttca atgtggtcgt gacgcggtcg attattgcgt tggcggtttt tctggcgata
     5881 ggcaaagcag gggcgtcgac gatgaaatcc gcgagtgtaa aatcctgaac cttcaattca
     5941 gccagatatt cgccgacggc ttttagcgta gtagatcgct tttcattatg cacgtgtgac
     6001 agcgggcagt cccaattttc gacgttatcc gctgactgaa gtgtgcattc tgcgcggccg
     6061 gatgcggtgg atatgctcag ccggtattcg ttagttgatt tatcctgtga tttttccgat
     6121 tttggcgaat taccgtcgat tgttgttgac gtctttggcc ggccggattc gccgcagaga
     6181 ttttcacgag acgcgtcgaa atatccgctt gtgaggcttc tgccggtata atcgccaagt
     6241 tcacgtgcgg tggttttaag gcttgtcgtt gcgccttcat cgagagcgtg gcgaaagatg
     6301 gagaccgctt tggcgaccca gtcagccttt ttcagtcggc cttctatttt gaggcatgtt
     6361 acgccggcgt cacgaatttc atcaagcttc tcaagcagtg aaaggtcgtg cattgaaagc
     6421 atacgtttcg gcgtgccgtc gccgttgagc cgccatgccg cccgacacgg acttgtgcac
     6481 gttccgcgat ttccgcttct gccgccgccc cagcttgaca gcagacagcg gcctgaaacg
     6541 caaaagcaca atgcaccctg tacgaagatt tccgtttcca cgcctttgcc tgaggcggcg
     6601 ttgatttctt cttttgaaag ttcgcgcgcc agtaccacgc ggtcggctcc gagttcgccg
     6661 gctgcctcga cgcccgctga attttcaatc gcgccctgcg tgctgaaatg aaacgccact
     6721 tcaggataaa acttccgcac tgccagaaaa agcgggtcgg tgaaaagtac ggcgtcgacc
     6781 ttggcgatac gtgcgacttc caatgcccgg accgcctggc cgacttcgcg ggctgcgata
     6841 tcggtgttga cggcgagaaa gacttttacg ccgttgtcat gggcatattt gactacgcgg
     6901 gttaattctt catggctgaa attcactgcg ccgcgtctgg catttaatga gcggatgccg
     6961 agataaacag catttgcgcc ggcttgcacg gcggcaacaa gagattcttt atcgccagcc
     7021 ggcgcgagca gttccggcgt gggtttgttg atattgtgct gcgtcattat ttcttttttc
     7081 gcatgaactt gctgatgtac gatatgtgat cctggatatt ttccgacacc atggcgtcgt
     7141 tggcttccgg tgttacggtg ttgaattgcg gcatttctga aggcggcaga tatcgctgaa
     7201 gcagcagttc gaattctcct atcgcctgcg agaaatggct ggcgttttcc agagcccagc
     7261 cgttttcata tatcgacatg attttgtcga taagctcttt tttggcagtg ttgtattgag
     7321 attcgtcttc tatctgaaaa gttttctggt caggcctgcg aaaagacagg tacagccggt
     7381 aatttttcac gcataaatag agatttttat cggtcgcatt tcgagtgcgg taatattccc
     7441 tcgccagacg gaggtgtttt gccgacgctt cggcattcgg tgcgtgattc aggtcgcacg
     7501 taagcgctgc agcgatggtt tcgcgggtca gaatagcagg cttttcgctg ttccctacag
     7561 ttgaggacga ttttttagcc agcgcaagaa aagtgaacaa accagccatg gctatcaggt
     7621 agacgatggc tgcgctgacg tataatttcg ttttgcgttt cttttctgtc gtagtgagct
     7681 tgtctgcggg agactttttt agacttgagg attccggttt gactgatttt ttttgagccg
     7741 gcggcgtctg ttgtgcctgt tcaggcgctg ccggcggcgg cgtttgagtc tggttggtat
     7801 ttgtgttcag atatttttcc atcgctgcga ttgggtcgtc accggagctt acgaacagca
     7861 gcgtcgtctc cgagcccagc gatatggtgt cgcccgtttc aagcagtacc tgtttgcccc
     7921 gcttgtacga ttggccggaa atttcgattt tattggctga gaggttttcc acaatccagc
     7981 cgtcgattgt aagtgcgaac cgcagatgct gacggctgac ggtttcttcg gtgattttta
     8041 tgtcagcctg cgatgaacgg ccggcaatga tattgttgtc catgaggacg gttcgctggc
     8101 ctttctgcgg gccggcgacg aaaatgagtt caggacgggc gtctggcatc agaaaagacc
     8161 tccacgcatt atgcgtatta tcgcagggcc gaagattgtg agtattacag ccatcacaag
     8221 cagcaggcag ggcacgagta tgcggacgct ggccgtggcg gccagatttt ccgcacgcac
     8281 cgaccgtcgc agtcgcagtg cggaggcctg gtcgtgtaaa acgttcgcca gcggtgtgcc
     8341 gagctgttgg gcctgcgtga cggcggaggc taacgactgt atcgaatcga gcggtacgcg
     8401 ctgtgccatg ttcgcaagtg cctgctgccg ggttgtgccg agttctatct cggcgagcat
     8461 cgcgcgaagc tcgacgttaa gcggctcgga atcttgcggg tcttcgtcaa ctatggtgcg
     8521 gacagcttcg gtaaaattgg ctcctgcgcc catggccagc gatatgaggt ccagggcata
     8581 gggcagttga cgggtgattg aacgcatgcg cttggcggac tggtctgcca gctggatgat
     8641 gcacagcgat gaaccgacgg ctatgccgag tattattaca gaaatgctcg gcgaacctgc
     8701 gacgagaatg tgtaataatt ccagtaccat gcctaatccg aagcccgtca tgaacgacag
     8761 ggccagaaat tcctgcggcg tataataatt cggattgccc gacgcggtga gctgtttgga
     8821 aatataccgc ttgagcgatt cggacctcag tctgtaactt atggccagaa atatccacag
     8881 aaacggcttg aacaggacgt tctcaaaaag tgttttgcgg tcggtatggc cggtggcgat
     8941 ggcggctgcc cgctgcggcg aaagcttgat ttcgccctgt tggccgaaaa cggctaatac
     9001 gaacatggat acggacagga acaggccggc cgacaacgcg acgtcccaac tccaccccaa
     9061 ccagaatgtt tctgaaattt ctgcaaatat catcgtttaa aatccgaaat tcaaatccgg
     9121 aacaaatccg aatttacaaa tatcaaatca ccaatcggaa tttgaaaatc aatcttggag
     9181 catcttattt gcaggacgct ctaaatatct atttccatta ttttccgtat ccacaggaaa
     9241 cccacaaggt tcatcaggaa aacagccgac agaatcagct tgccgatgtc gtctgtaaaa
     9301 agtttttcca cgtttgaacc aggtatgaaa tacattatgc acatgacgac gagcggcatt
     9361 gcgcccaacc atcttgcggt ggctcggccc tgtgcggtca gcgagtcaac tcttgcgttg
     9421 agccgctgga tttcacgtat cgaatctgca atcctgtcca gcgtatcgcc caggtcgccg
     9481 ccctgttgcc tgtgcgtgtg gagcgcggtg aacagcagcc tgtaatctgc cgagccgatt
     9541 cgctttgccg tcgaaagcat ggcttcgtcg agcggcgtgc cgtattcata ttcgcttaga
     9601 agatgtgaaa attcctggct tatcggggct ggcgcgtcgc gggcgatgag ttccatcgcc
     9661 tgtaccaggt tcaaaccggc tcttacgccg gacgccagcg tctgaatgcc ctcgactatc
     9721 tgcgtttcga gcctgtcgag tcgttttttt tgcagcttgc gtataatcgc gccaggcaac
//
LOCUS       DAONFI010000043         9779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7923_length_9779_cov_3.520259, whole genome shotgun sequence.
ACCESSION   DAONFI010000043 DAONFI010000000
VERSION     DAONFI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7923_length_9779_cov_3.520259"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLK08_02370"
     CDS             <1..511
                     /locus_tag="PLK08_02370"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPS52373.1"
                     /translation="PDVDFRKPDQVLCGLNEAFQMEDHNDMFFTIWYGVYNKKTRELQ
                     YASGGHHPAILIEPTDAGGRNMMQLISDGMLIGAMRGIKFNSGICTVKPGSRLYVFSD
                     GVFEIPIKAKDGKMWNMEEFFNLLNQPSVNDASNVTRLLGEVRNIQGRDEFNDDFSLL
                     EIMFSSELS"
     gene            complement(516..1871)
                     /locus_tag="PLK08_02375"
     CDS             complement(516..1871)
                     /locus_tag="PLK08_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HPS52374.1"
                     /translation="MNVKAMAVFVLLVVCNCANAQETAAEKKTGFLDMGVTLTEQSGQ
                     DYNDSSNPLNSGNYRKYAAGKTYYINRMPYTYRLDGMPDPVFGHGFISTSFRKWYEMC
                     KQLELNAGLHLDISYTIWGQWASKRVSGVSGKATPPGLTTGRLDVQGYWDLPQNNPLG
                     NSRVNFRMRQTTNYGWSDGTTLDTTIGDVSGVNGGYDPSIAMMVLSYGQELFDGAVDV
                     EVGKVHPGMYFFASPVADDETSQFINNVLDGSPCQVINNYAPGAIIRINELFGRKDMY
                     LYAAAIDMADTSTTCAGGIGRGKWGVVTEYGWTPKFEDLQGHYRFDWWYADNTHFGFN
                     SGQGAGLNFDQEIAHNCILFLTYAYNANDSYSQEHQLGYGVGILEPFKRRGEMFGVGG
                     GWAKATENGADEQLLETFYRFQLTDSIQFSPDLQMIFNPAYAPDTSVCFVLGFRVKVQ
                     F"
     gene            complement(1889..3280)
                     /locus_tag="PLK08_02380"
     CDS             complement(1889..3280)
                     /locus_tag="PLK08_02380"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HPS52375.1"
                     /translation="MALHERNRIRENLLDDVYASTDISTALPKYKLPKNEHEPRHIYQ
                     LVHDELMLDGNSRQNLATFCQTWVDPEIHDLMNECIDKNMIDKDEYPQTTELECRCVH
                     ILADLWNSPEAANTLGCSTTGSSEAAMLGGLAMKWSWRAKMKAKGKPTDKPNMICGPV
                     QICWHKFARYWDVELREIPMEGDRLLMSPEEVIKRCDENTIGVVPTLGVTFTGQYEPV
                     KAVSDALDKLQKDTGIDVPIHVDAASGGFLAPFTDPELLWDFRLPRVVSINASGHKFG
                     LSPLGVGWVVWRDKAHLPEDLIFHVNYLGGDMPTFALNFSRPGGQIVAQYYNLLRLGH
                     QGYRKIQKACYDTATYLGKEVDKLGPFKVLYNGKGGIPAICWTIKDGLKPGYTLYDLS
                     DRLRSRGWQVPAYSMPAHREDMVVQRVLIRHGMTRDLADLLLDDMRRCMEYFEKNPVK
                     VSLTSKSGGYSHT"
     gene            complement(3319..4761)
                     /locus_tag="PLK08_02385"
     CDS             complement(3319..4761)
                     /locus_tag="PLK08_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPS52376.1"
                     /translation="MSGKGKYLTVFALAMMNVAAVFSLRGLPMLADQGWTVIFYIFFA
                     TVLFLFPVSMVAAELASGWPQSGGVFRWVKEAFGDRWGFVAIWIQWIQNVIWYPVQLA
                     FIAGCLAYLCFDSKLAASKEFTLGVVVIVYWAATLLNFFGVGWAAKIASLGVIIGTVI
                     PAVGLVALSIYWLATGHSMAIDAVSTPVIPDLTKFSNVSFLGGIVLLFAGMEVCAVHV
                     QELKNPGRDYLRSLIIAMIVIVLVAFLGSFAVAVVVPAKGLSLTSGVLQCFNDAVGNC
                     KYLHEYIGAIGLPWLLAILGGMIAFSATASVIAWLAGPSKGLLATREYGCIPPFMQKV
                     NKRGVQKNILLLQGAMVTILGFVYVIMPNVGSAFVLLATLASMLYLIMYLFMYAAAIK
                     LRISQPDVPRSFKVPGGLPGMIFFSGIGILAVLFAIVVSWVPPKNLSVGTPATYVLVL
                     IIGVVVLGGAPLLIYACRKPHWISKDTQEN"
     gene            complement(5017..8169)
                     /locus_tag="PLK08_02390"
     CDS             complement(5017..8169)
                     /locus_tag="PLK08_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52377.1"
                     /translation="MLDEMIRDFSEAGCEYRAKPFWAWNGKMLPEELRRQIKIMKRMG
                     LGGFFMHSRVGLDTQYLGDEWFECVEACVDEAKKLDMQAWLYDEDRWPSGAAGGLVTK
                     NPEHRHKYLSVEIFDGPTDVKNENGFMAVFVAKLKGAKASDVKRRQCGSITPAAGEKI
                     LVFRQKTSDCSSWYNGYSYLDVMSEEAVAAFIESTHEKYLENCGDDFGKAIPGIFMDE
                     PNHGPYFARIQEIGNVDGVPWTPKLPEIFKARYGYDILDRIVEVFFDVTDLDEQGITP
                     ARHDYHDCTTYLFCTAFGRQLYEWCEKHGIDLTGHVLFEDTLYHQSQVVGSCLRFYEY
                     MQTPGIDLLTQHWTIFETARQVTSAAHQFNRKRRLTETYGCTGWDFEFAGHKALGDWQ
                     AAMGINLRCPHLSWYTMLGEAKRDYPASIFYQSPWWQQYVAIEEYFARINAAMSGGCE
                     CRDVLVIHPVESAWMCVRHGWNVEPEKYGDVSHNLDVEFKKLTRSLDAANVEFDFGDE
                     EMMSRLTKISDGRITVAHASYKAVIVPQMITIRSTTLELLRKFRAAGGTVIFTGDAPQ
                     YVDAVRSDAAAEFAGSCVMLDASMKNIEQVAGGYRIVSIFDDRGNEIPDALHLLREDD
                     EAFYLFICNTAADYVPDAKGDCEMSRLKVVERKRGYESVAVKGFSACKGKPVELNPLD
                     GTMTFADAKKCGDGWTINTSLPALASRLFVIPKEKKSRLAAKTVKPLKNISSRKIGGK
                     KWDYVLSENNVLVLDMPKFRINGEKWQKPLEILQIDRKVRTAMNINSRGGEMVQPWVR
                     TKNPNPTSKHVELEYEFDIKTLPSGELSFAIEMPSLYKVFVNDIELDRTCESGWWVDL
                     SLRRLPVDPTMLHIGKNVIRLETDYNENHPGFECVYLLGNFGVVVKGCRTTMTSLPER
                     LDLGTWTKQGLPFYSGNVGYHTMVKKPSAGKRAFIKIGRYCGVAVEVLVNGCSAGVVG
                     WGAGDEAEITKLLNMPQNEVTVKILGHRRNSHGPLHLGKDVIGIGPYAFVPGGGIEWS
                     DDYVLKDIGMLEQPTLKICE"
     gene            8312..9118
                     /locus_tag="PLK08_02395"
     CDS             8312..9118
                     /locus_tag="PLK08_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS52378.1"
                     /translation="MALSLQRRQNDIGLHTHEFMELVITTSGRGLHYTQDEEYPIRRG
                     DVFVIQPSHPHGYRQTRSLCIMNILFDLSWFDRLTAEIPKKQGFYFLFQSDRHRDTSH
                     MQLSDSELKKVQSLLSSIQKQMPAIKSSGNNLIAAGLLLEIAGFLSTCYKSDAPTSVS
                     VLQTDAVMEYIEKNYADEISLAQLSDIAKISSRNLLRIFRRATGTTPVDYLLHYRISR
                     ACRLLTETDKPVTKIAFDVGFSDGNYFCRQFKKIIRISPARYRAMQALLK"
     gene            complement(9425..>9779)
                     /locus_tag="PLK08_02400"
     CDS             complement(9425..>9779)
                     /locus_tag="PLK08_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HPS52379.1"
                     /translation="DIKLAWMVDQSHNIKPKIQAQIQTVMAIQELFAKALLVDRKALA
                     AAQAAEDVIGAETVLRDAFTADVSGILKAVRKNLGVPANPLKAFRDSGYEEKAAKTRI
                     QKRKDLGINEGGAFA"
BASE COUNT         2519 a         2692 c         2177 g         2391 t
ORIGIN      
        1 gccggacgtg gatttccgca agccggacca ggtactctgc ggccttaacg aagcgtttca
       61 gatggaagac cataacgata tgtttttcac tatctggtac ggcgtttaca acaaaaaaac
      121 cagggaactt caatatgcat ccggcggaca tcaccctgcc atacttatcg aacccaccga
      181 tgccggcggc cggaacatga tgcagttgat aagcgacggc atgctcatag gcgcaatgcg
      241 aggcataaag ttcaacagcg gcatatgcac cgtcaaacca ggcagccgtc tatacgtctt
      301 ttccgatggt gttttcgaaa ttcctatcaa ggctaaagac ggcaagatgt ggaacatgga
      361 agaatttttc aatctgctca atcagccgtc ggtcaacgac gcttcaaacg tcaccagact
      421 gctaggcgaa gtacgcaaca tccagggccg cgatgaattc aacgacgatt tttcactgct
      481 tgagataatg ttcagttctg aattgtcata aaaaatcaga actgaacttt tactcggaaa
      541 cccagtacga agcatacgct cgtgtccggc gcatatgcag gattgaatat catctgtaaa
      601 tccggtgaaa attgtatgct gtccgtcagc tgaaaacggt aaaacgtttc gagcagttgt
      661 tcatccgcac cgttttcggt agctttggcc cagccgccgc cgactccgaa catttcgcca
      721 cgacgcttga acggttcaag tatgccaacg ccgtaaccaa gctgatgctc ctgcgaatag
      781 ctgtcattcg cattatacgc atacgtcaga aacagaatgc agttatgcgc tatttcctgg
      841 tcgaagttca ggcctgcacc ctgtccgctg ttaaaaccaa aatgggtgtt atccgcgtac
      901 caccagtcga aacgataatg cccctgcaaa tcttcgaatt tgggcgtcca gccgtattct
      961 gtcacaacgc cccacttgcc gcggccaatt ccgcccgcgc aggtagtcga agtatcagcc
     1021 atgtcaatcg cagcggcata caaatacata tccttgcggc cgaaaagttc attgatacga
     1081 attatcgcgc ctggagcgta attgttaatc acctgacacg gcgaaccgtc aagtacattg
     1141 ttgataaatt gcgacgtttc atcgtccgct accggacttg cgaaaaaata catacctgga
     1201 tgtaccttac cgacttcgac gtcgaccgca ccgtcaaaaa gctcctggcc atacgacagc
     1261 accatcatgg caatcgacgg gtcgtacccg ccgtttacgc cgctaacatc gccgatagtc
     1321 gtatcgagcg tcgttccgtc cgaccagccg taattcgtag tctgacgcat acggaaattc
     1381 acgcgagaat tgccgagcgg attgttttgg ggcaaatccc aatatccctg aacgtcaaga
     1441 cggcctgtcg taaggccagg cggcgtcgcc ttgccgctga cgccggaaac acgtttgctg
     1501 gcccattgac cccatatcgt gtatgaaata tccagatgca gcccggcatt aagttcaagc
     1561 tgcttgcaca tctcgtacca ttttctaaaa gacgtactga taaagccatg gccgaaaacc
     1621 gggtcaggca tgccgtcaag cctgtaggta tacggcatac ggtttatgta atatgtcttg
     1681 ccggcggcat atttacgata gtttcctgaa ttcagcggat tactgctgtc attataatcc
     1741 tgacccgact gctctgtgag cgtcacgccc atatctagaa aaccggtctt tttttcagcg
     1801 gcagtttcct gggcattggc acagttacag acaacaagca gcacaaaaac tgccatagct
     1861 ttcacattca ttgtcacatc actccatttt acgtgtgact gtagccgccg ctcttcgacg
     1921 taagcgatac tttaacaggg tttttctcaa aatattccat gcagcgacgc atatcgtcca
     1981 gcagcaggtc agccagatcc cgcgtcattc cgtgacgtat caggactcgc tgaacgacca
     2041 tatcttcacg atgagccggc attgagtacg ccggcacctg ccaaccgcgg ctgcgaagcc
     2101 tgtccgacaa atcataaagc gtatatcctg gttttaagcc gtccttgatt gtccagcata
     2161 ttgcaggaat gccgcccttg ccgttataca gaaccttgaa cggcccaagt ttgtcgactt
     2221 ccttgcccag ataagttgcg gtgtcatagc acgccttctg tatcttgcga tatccctgat
     2281 ggccaagccg cagcaggtta taatattgtg caacaatctg gccgccgggc cgcgaaaaat
     2341 tcagggcaaa agtcggcata tcaccgccga gataatttac gtggaaaatg aggtcttccg
     2401 gcagatgtgc cttgtctctc cacaccaccc agccgactcc cagcggcgaa agaccaaact
     2461 tgtgaccgga ggcattgatg gataccacgc gcggcagacg aaaatcccac agcagttcag
     2521 ggtcggtaaa cggagcgagg aaaccgccgc tggccgcatc cacgtgtatg ggaacatcaa
     2581 tgccggtgtc tttctgaagt ttgtcgagcg cgtcactgac agcttttacc ggctcatatt
     2641 ggccggtaaa cgtaacgcca agagtgggca cgacgccgat tgtgttttca tcgcaacgtt
     2701 tgataacctc ttcaggagac attaacaggc gatcgccttc cataggaatt tcgcgaagtt
     2761 cgacgtccca gtatcgtgcg aatttgtgcc agcatatctg caccggcccg catatcatgt
     2821 tgggtttgtc ggtcggtttg cctttcgcct tcatctttgc acgccagctc cacttcatag
     2881 ccagcccgcc aagcattgca gcctcgctcg aacctgtcgt cgaacagccc agagtattgg
     2941 ccgcttccgg agaattccaa aggtcggcaa gaatatgaac gcaacggcat tcgagttccg
     3001 tcgtctgcgg atattcatcc ttgtcaatca tattcttgtc tatgcattcg ttcatcaggt
     3061 cgtgtatttc aggatcaacc cacgtctggc agaacgtagc gaggttttga cgcgaattgc
     3121 cgtccagcat caactcgtca tgtaccaact ggtatatgtg tcgcggctca tgctcatttt
     3181 tgggtagttt gtatttgggc aatgccgttg aaatatcggt tgacgcataa acatcgtcca
     3241 gcagattttc acgtatcctg tttctttcat gtaatgccat aatatcatct cctttgaaat
     3301 ttcatgcata tccggcaatc agttttcctg agtgtcttta cttatccaat gcggtttgcg
     3361 gcatgcgtat attagcagcg gagcgccgcc aagcaccact acgccgatta tcaacaccag
     3421 tacataagta gccggtgtgc cgacggaaag atttttcggc ggcacccaac tgacaacaat
     3481 ggcaaaaaga accgccagaa tgccgatgcc ggagaaaaat atcatccccg gcagaccacc
     3541 aggcactttg aatgaccgcg gcacgtcagg ctgacttatg cgtagcttta tagccgctgc
     3601 gtacatgaac agatacatta taaggtacag catcgacgcc agcgtggcta gcagcacgaa
     3661 cgctgaacca acattgggca ttatgacgta aacaaatcca agtatcgtaa ccatagcgcc
     3721 ctggagcaga aggatgtttt tctgtacgcc gcgtttgtta accttctgca tgaacggcgg
     3781 aatacagcca tattcgcgag tcgccagcag acctttcgat gggccggcta accacgcgat
     3841 tacagacgcc gtcgcagaaa acgctatcat gccgcccaat atcgccaaca gccacggcag
     3901 gccaatagct cctatatatt catgcagata tttgcagttc ccaacggcat cattgaaaca
     3961 ttgcagaaca ccgcttgtca gactcagccc tttagccggc acgaccaccg ccacggcaaa
     4021 tgaaccaagg aacgcaacaa gcactatcac tatcatggcg attatcagtg aacgcagata
     4081 atccctgcca gggtttttca gttcctgtac gtgtacggcg cagacttcca tgccggcaaa
     4141 gagcagaact atcccgccga gaaatgacac attgctgaat ttggtaaggt ctggtattac
     4201 aggagtactg accgcatcga tagccattga atgtcccgtc gctagccagt atatgctcaa
     4261 cgccaccaag ccgactgctg gaatcaccgt gccaataata acacccagcg atgcaatctt
     4321 ggcagcccac cccacgccaa aaaaattcag aagcgtcgcc gcccagtaaa ctatcactac
     4381 cacgccaaga gtaaactctt tgctggctgc gagttttgaa tcgaaacaca gatacgcaag
     4441 gcagccggcg ataaaagcaa gctgcacagg ataccatatg acgttctgaa tccattgaat
     4501 ccagatagcc acgaatcccc agcggtcgcc gaaggcttcc ttgacccagc ggaatactcc
     4561 gccggattgc ggccaaccgg aagcaagctc cgcagccacc atggacacgg gaaacagaaa
     4621 aagcacagtg gcaaaaaaaa tgtaaaaaat caccgtccag ccctggtcag ccaacatggg
     4681 cagtcccctc aaactgaaaa cggctgccac attcatcatc gccagcgcaa aaaccgtcaa
     4741 gtatttgccc ttaccgctca taaatcatct cctttaaaaa tggaactatt cgtgcaatgt
     4801 catgcatgat tatagccaaa atgtcagtat ccatttgaaa accctcaact taaatatcta
     4861 atatagagca tgccaaaaac tataacaaga aaaacgataa attttttata ttataatttt
     4921 tcccgatttt gtcttgccgc attgcagcaa atataaaaaa agacagtgcg tcgttcaaac
     4981 gcactgccct gaaataatgt accatgtaaa taaaaatcac tcacatattt taagagtcgg
     5041 ctgttcaagc atgcctatgt ctttcagcac gtagtcatcg ctccattcga ttccgccacc
     5101 tggaacgaat gcataggggc ctattccaat gacatccttg ccgagatgga gcggcccgtg
     5161 gctgttgcga cgatggccaa gaattttaac tgtcacttcg ttttgcggca tattcagcaa
     5221 tttggtaatc tcagcctcat cgccggcacc ccatcctacc acgccggcag aacagccgtt
     5281 aaccaacact tcaaccgcca cgccgcaata tctgccgatt ttaatgaacg ctctcttgcc
     5341 tgctgacggt tttttaacca tcgtatgata tccgacattg ccggaataga acggcagccc
     5401 ctgcttagtc cacgtaccca aatcaagtcg ttcgggcaga gaagtcatgg tcgttctgca
     5461 accttttacg acaacgccga aattacccag cagataaaca cactcaaatc ccggatgatt
     5521 ttcattgtag tccgtttcca gccgtatgac gtttttccca atatgcaaca tcgtcggatc
     5581 gacaggcagc cgtctgagtg acaaatcaac ccaccagccg ctctcgcagg tgcggtcaag
     5641 ctcgatatcg ttgacaaaga ccttataaag cgacggcatc tcaatagcga agctcagttc
     5701 accggacggc aatgttttta tgtcaaattc atattcaagt tcaacatgct tcgacgtcgg
     5761 atttggattt tttgtccgta cccacggttg caccatctcc ccgccgcggg agttgatatt
     5821 catggcggtg cgtaccttgc ggtcaatctg gagtatttca agcggctttt gccatttttc
     5881 accgtttatg cggaacttgg gcatatcaag tacaaggacg ttattttccg aaagcacgta
     5941 atcccacttt ttgccgccaa tttttcgtga tgaaatattt ttgagcggct tgactgtttt
     6001 ggcggctaat cgcgatttct tttccttagg aatcacgaat aaccggctag ccagcgccgg
     6061 caacgaagtg tttatcgtcc atccgtcgcc gcatttcttc gcatcggcaa acgtcattgt
     6121 gccgtcaagc ggattaagtt caaccggctt gcctttgcac gctgaaaaac ctttcacggc
     6181 caccgactca tatccacgtt tacgttcaac aaccttcagg cggctcattt cgcaatcgcc
     6241 ttttgcatcg ggtacgtaat cggctgccgt attacaaata aaaaggtaaa acgcttcgtc
     6301 atcttcgcga agcaggtgca acgcatccgg aatttcattc cctctatcat cgaatatcga
     6361 aactatccta tatccgccgg cgacctgttc aatatttttc atagaggcgt caagcatgac
     6421 gcacgaccct gcaaattcag ccgccgcatc actccgcacc gcgtcaacat attgcggcgc
     6481 atcgccggtg aaaatgaccg ttccgccggc agcacggaat tttcgtaaca attccagggt
     6541 agtgctgcgg atggttatca tctgcggcac gataaccgct ttatatgacg catgtgcaac
     6601 cgttatccgg ccgtcggaaa ttttcgtcag gcgggacatc atttcctcgt cgccgaaatc
     6661 aaattcgaca ttggcggcat caagggagcg ggtgagtttt ttgaattcca cgtcaagatt
     6721 gtgtgaaacg tcgccatatt tttccggctc aacattccag ccatgccgca cgcacatcca
     6781 cgcgctttcg accggatgaa tcacaagcac gtcccgacac tcacagccgc cggacatggc
     6841 tgcgtttatc ctggcgaaat actcctcgat ggcaacatac tgctgccacc acggcgactg
     6901 gtagaaaatg ctggctggat aatcacgctt agcctcgccg agcatggtat accatgacag
     6961 atgcggacat ctcagattta tgcccatggc cgcctgccag tcgcccaagg ccttgtggcc
     7021 ggcaaattca aaatcccaac cggtacagcc atacgtctcc gtcagccgtc ttttgcgatt
     7081 gaactgatgc gcagccgacg taacctgccg ggcagtttcg aatatggtcc aatgctgcgt
     7141 cagcagatca atgcccggcg tctgcatata ttcataaaaa cgcagacaac ttccgaccac
     7201 ctgcgactgg tgataaagcg tatcctcaaa cagcacatgg ccggtcaaat caatcccatg
     7261 cttctcgcac cattcataca gctgtcggcc gaacgccgta cagaaaagat acgtcgtgca
     7321 gtcgtgataa tcgtggcgtg ccggcgtaat gccctgctcg tccaaatccg tgacatcgaa
     7381 gaaaacctcg acaatcctgt ccaaaatatc gtagccgtag cgggctttga aaatttccgg
     7441 caatttcggc gtccacggca cgccgtcgac attgccaatt tcctgaattc tggcgaaata
     7501 cggcccatga ttcggctcat ccatgaatat gcccggaatc gccttgccga aatcatcgcc
     7561 acaattttcg agatattttt cgtgcgtact ctcaatgaat gccgccaccg cctcttcgct
     7621 catcacgtcc agataggaat aaccattata ccacgatgag cagtcggacg ttttttggcg
     7681 aaagaccagg attttttcgc cggctgcggg cgttatcgag ccgcactgcc gccgtttgac
     7741 gtcggatgcc ttggcgcctt tgagttttgc gacaaacacc gccatgaaac cattttcatt
     7801 tttgacgtca gtcggtccgt caaatatttc aacagacaga tatttatgcc ggtgttccgg
     7861 atttttcgta acaagtccgc cggcggctcc gctcggccaa cgatcttcat cgtacaacca
     7921 agcctgcatg tcgagttttt tcgcttcgtc gacacaagcc tccacgcatt caaaccattc
     7981 gtcgccgaga tactgcgtat caaggccgac gcgagaatgc atgaaaaaac cgcccaaccc
     8041 catacgtttc attatcttta tctgcctgcg cagctcttcc ggcaacattt tgccgttcca
     8101 cgcccagaaa ggtttggcgc gatactcgca gcctgcctcg gaaaaatccc taatcatttc
     8161 gtcaagcata ttggtacact ccaaaaagta tcgccacagt gtacagtgtt tttaggggaa
     8221 aagccttgat tatttcgaca atttatagca ttatatcgcc atgccttcat ccagttacaa
     8281 atccaataat tacttctccc atgaattccc catggctctg agcctccaaa ggcgccagaa
     8341 tgatatcggc cttcatacac atgaattcat ggaactggtc attaccactt ccggccgagg
     8401 tctgcactac acgcaggatg aggaatatcc aatacgccgt ggagatgtct tcgtcattca
     8461 gccgtcgcat ccgcacggct accgtcagac acgttcgttg tgtatcatga acatattgtt
     8521 cgatttgtcg tggttcgacc gtcttactgc ggaaataccc aaaaaacagg gtttttattt
     8581 cctttttcag agcgacaggc acagagacac cagtcatatg caactcagcg acagcgaact
     8641 gaaaaaggta cagtcgctcc tttccagcat acagaagcag atgccggcta taaaaagcag
     8701 cggcaacaat ctgatagccg ccggcttgct gcttgagata gcaggatttc tttcgacatg
     8761 ctacaaaagc gacgctccga cgtcggtttc cgtcttacag acagacgcag taatggaata
     8821 catcgaaaaa aactatgcgg acgaaatttc gctggctcaa ctatccgata tcgccaaaat
     8881 ttcatcacgg aatctgttga gaattttccg gcgggcaacc ggcacaacgc ctgtggatta
     8941 tcttctccat taccggataa gccgtgcctg ccgcctgttg acagaaaccg acaaaccggt
     9001 aactaaaatc gcgttcgacg taggcttttc cgacggcaac tatttctgcc gccaattcaa
     9061 aaagataata cgtataagtc cggctcgcta tcgtgcaatg caggcactgt taaaatgaaa
     9121 cgccataaaa attttcacca gagcatatta gagcggttct caaaattatt ttccttttga
     9181 agcttgctgc aagtttccct gctattgtcc gtctgcatcg gaaaccatgc aggttgcctg
     9241 cttcgacgtc cttagcagga aaactttcgc tgacgctcaa aaagaaaatt ctttagagaa
     9301 ccactctaaa cctgactttg cgtttgctgc tgtttgcggc aggcatgttt gatggacagg
     9361 ttctacaaaa aaagagcggc caattttagt tggtcgctct tgttctgttc tcacaaaaat
     9421 caaattacgc gaaagccccg ccttcattaa tgccgaggtc tttacgtttc tgaattcgcg
     9481 ttttggcggc tttttcttca tagccgctat cgcggaaggc tttgagcggg ttcgccggca
     9541 caccgaggtt tttgcggaca gccttcaaaa tccctgaaac gtcagccgta aacgcatccc
     9601 gcaaaacagt ttctgcgccg attacgtcct cagccgcctg tgccgccgcc agagccttgc
     9661 ggtcaaccag caatgccttg gcgaacagtt cctgtattgc catgacagtc tgaatctgag
     9721 cctgaatttt cggcttgata ttgtgtgact ggtcaaccat ccacgcaagt ttgatgtct
//
LOCUS       DAONFI010000044         9727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_7988_length_9727_cov_3.800558, whole genome shotgun sequence.
ACCESSION   DAONFI010000044 DAONFI010000000
VERSION     DAONFI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7988_length_9727_cov_3.800558"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLK08_02405"
     CDS             <1..861
                     /locus_tag="PLK08_02405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPS52380.1"
                     /translation="SENTDNKCCICGHVSQNPEQTSIRSNVRKFRNEIFELWRCEKCR
                     SIHSKSDVNLDYYYSYYPFFKQTLDGILKMSYRRLTHRLKRAGMSRTWKILDYGCGSG
                     LLMEYMRTDGYDAVGYDRYSADHSDPIILNRQYDLVVAQDVIEHAADPLEILRQLDSL
                     VRPGGQIAIGTPNAAGIDLKNPEKFVHPLHQPYHRHIFAIDTLIQCAADLGWSLQAYY
                     PTPYTNMPLLSLPFLHHYMKQYDGCIDMLFEPNKKIGRLLYHPGTLFWLLAGYFLCND
                     ADIVAIFHKR"
     gene            complement(1320..1979)
                     /locus_tag="PLK08_02410"
     CDS             complement(1320..1979)
                     /locus_tag="PLK08_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52381.1"
                     /translation="MYRQLIESFKNDLRTNPKMVKIMAGVLCAVLVVAITVIVISAYE
                     PAAPNPEIAKLNDVERYISSEAFLKMPVTERKEYINRVTDRIKSSNGEEEMGGFLKRL
                     YAKNQHAGDVVQINMVSERLRPICQLDPKEQAVKINTMLNVAEMTQGYDRMYQVFNAM
                     FGNDSPERKKKILSRSVLAMPLGMKTTTPRERKQIMQTLKITKMQMQKRYGKPLPQPQ
                     K"
     gene            complement(2025..2669)
                     /locus_tag="PLK08_02415"
     CDS             complement(2025..2669)
                     /locus_tag="PLK08_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS52382.1"
                     /translation="MGLVMKDTHISNKGFTLIELLVVISIIALLVSIIMPSLGTARQL
                     AQRVACSATLKGIGNAWAMYLDTNNRCMPYKDTDGESIELYPYEKDSGNYNIMVAMKD
                     YVGEGVFWKCPSIIGSENYQMYGSSYEYVMTYMAGLMDDVGAEFFRINAEHFPDKIPI
                     FYDAQSDAGEAAHASKQKPDGQNHMFNDSHVEYASWDDISDNLNAIKSQLSYPE"
     gene            complement(2756..3982)
                     /locus_tag="PLK08_02420"
     CDS             complement(2756..3982)
                     /locus_tag="PLK08_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52383.1"
                     /translation="MENRRDNSDALWDAVLGELGRRERLSPSPSVRQTTCWAETIASS
                     AGKTAPRRSHAVKWRVWGTYVAAAAVLVMAGLVGIFVNRNSLTADEVFYSVDNTITAS
                     QVVKFSVCGLQMQKHVINMDFMCSNTDENAYVGLNIRPLSGHVGKDAVSADIVYISQN
                     STGLLLVKKLSVGGVDVLADMNQCDSGSSREIAIRFDDISGSSATRRFITGRIELKNI
                     QELINSLKNSASDFQITRIDGNRSEMTGVIDKPQNISIKALMQAAMMASYANMLVLDA
                     GIEASNSAMTPAAKKNFIAVSHILMETRLRALLRIMPRQVDRLFRFVALCQLYSNGSM
                     DDNADFIKYRTRIDSILNAMRVTLVYDRSSNLIRRVTLKKSDGASVNIRFATNDELKF
                     MMSQVVNESKKIDGGH"
     gene            complement(4042..4623)
                     /locus_tag="PLK08_02425"
     CDS             complement(4042..4623)
                     /locus_tag="PLK08_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPS52384.1"
                     /translation="MKALWRKKDNLKSSDRPKTVAGGGIETLFLRNATGLWRFFLHRS
                     GSESNAEDLMQELCLQLARTELPAGDDDFQDAWLFGVARNVWRNFIRGMLRRRNNIPM
                     ATAFDGMELVERVERGDRPEDILADDEKRQKLMTAVTSLAGIDQEIIRLHYFEGFSQS
                     EVAAAVGISERAVEGRLYRARQTLRAKLADNDL"
     gene            complement(4883..5950)
                     /gene="purM"
                     /locus_tag="PLK08_02430"
     CDS             complement(4883..5950)
                     /gene="purM"
                     /locus_tag="PLK08_02430"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPS52385.1"
                     /translation="MSEKSITYKQAGVDIDAGDEMVDLIKPMVRSTFSPRVMDNFGSF
                     AGLFRLDFGEKLFKKKYKDPVLAACTDGVGTKLMIAFGMGVLNTVGIDLVAMNVNDLI
                     CGGAEPLFFLDYLGVGRLDPKKMAEIVSGITDGCRQSNCSLIGGETAEMPSFYKPDEF
                     DMAGFAVGVVERSRIIDGGRAKAGDVVIGLAGNGVHSNGYGLARRVLLEIAGYKLSDK
                     PEELEGCSIGEELLKPTRIYVQPILRLLDHYESTMPVKAMAHITGGGLPGNVPRVLPK
                     GLTVRIKRDSWPIPPVFKLIAKKGPVDMSEMFRVFNMGIGYVVIVSPRYARSVMAQLS
                     ECGERCYQIGKVVKGGELQWN"
     gene            6173..8620
                     /locus_tag="PLK08_02435"
     CDS             6173..8620
                     /locus_tag="PLK08_02435"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS52386.1"
                     /translation="MARIVFTTFLSIAAVMTFAGLGGCDAPVEKKQPSPAGQTITASE
                     IAEFDTLRQQLLQGNRTEQTRLDAARILLSKTYPQAAELLCRLLYDAARPTTRVAVAQ
                     AISEESSPRTEFYDPLMDMLKSDLSNVQLAAAQALASFNDHKTVTSLIEIAENPKKPR
                     EMRLAVITAMRSCIQPESIESLIELLKDHDKVIRNTATESLEHLTGMESFGINYRRWR
                     SWWDAREFQTKTQWLETLDQNLMRNQIELERKNALLAERLTQTLQDLFNSAPKNSKND
                     MIQQMLADALPEVRLLGVKLAMKLIDTGQTLPDQTVSRLMAMMYDSSAQVRQAAATVL
                     ADLNHPGSAKTLLNCLKTETYPEVRVAILEELGQLKKPLTLPAIIQSIASEDDQESAA
                     AAQALTQLAQAHLIAQPAEISQATSALIARYESLLNNENSYALREALLTAMGSLAAPA
                     AKTVLQNAVNSKSGAIRLAAIAGLEQYKDRSLAPIFAEKIHDSDRGVRLAAVKALTGA
                     NDRKYLETLIMMASPKVESDLAIRQAAAQAALEICRNADAGTLVTTLKLLRQQQQIDS
                     SLKIELLQLYLKSLTNQETPRKLMVELDLADELVLNSQPNDAAVLLSQAVRLAKKLAQ
                     TSPVSPAEIWEKYIQLLIDTGAPETIKEIADQNDPKQRKVAIEHILSWIRSPAADKNL
                     TATITVTDGTLTKLGEDLTDGQKKALRGKMLAAVKTQQVIDRQQVARLLTDYMGTDGV
                     KSADALKQIKAMGDRALKPLLESLKNVIGGEKANPDQEKSIIMLIKQVAPQVGDYDPN
                     EPAAQKITRINDWLKKL"
     gene            8665..9648
                     /locus_tag="PLK08_02440"
     CDS             8665..9648
                     /locus_tag="PLK08_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPS52387.1"
                     /translation="MNRVCIGIDFGGTFIKFGTLAEDGVTTSEILQLPTPVKDGPAAI
                     IAQIKNGVRQIIERDSLSHENIVALGIGSPGPLSVKRGIILNLPNIPNMQNIPIARII
                     GEEFDIPAALENDANAAAYGEYLCGAGKGCENIVMLTLGTGVGGGVIQENRVLHGTHE
                     IGGELGHMIVQPGGRPCGCGQRGCLEQYCSAAFLAKYTVEKIKTQNPDSSLTKTYRDS
                     GELTALDVNLAQQAGDAFAIECWDECCYYMAIGCINLCRIFDPDRIVIAGGMSKAGDE
                     LINPTLKYIREFNWRMFEPQTPLAVASLGNSAGMIGAAGVAWLTFGEKTFE"
BASE COUNT         2492 a         2631 c         2305 g         2299 t
ORIGIN      
        1 tcagaaaaca cagataataa atgctgcata tgcgggcatg tctcgcaaaa tccggaacaa
       61 acatcaattc gctcaaacgt ccgtaagttt cgcaacgaga tttttgaatt atggcgatgt
      121 gaaaaatgca gatccataca ttccaaatcc gacgtaaatc ttgattatta ctatagttat
      181 tatccgtttt tcaagcagac gctcgacggc atattaaaaa tgagctatcg ccgcctgaca
      241 catcggctca agcgtgccgg catgagcagg acgtggaaaa ttctggatta tggctgcggc
      301 agcggccttc tgatggaata tatgcgaacg gacggttatg atgccgtcgg atacgaccgc
      361 tactccgctg accattctga cccgattatt ctgaaccgcc aatatgacct tgttgtggcc
      421 caggacgtca tcgagcatgc cgccgacccg ttggaaatcc tgcgacaatt ggattcgctg
      481 gtccggcctg gcggccaaat cgcaatcggc acgccgaacg cagccggcat agacctgaaa
      541 aatccggaaa aattcgtcca tccgctacat cagccgtatc acaggcatat cttcgccatt
      601 gatacgctca tacaatgcgc cgccgacctg ggctggtcgc tacaggcgta ttatcccact
      661 ccatatacaa atatgccgct cctgtccctg ccgttcctgc atcattacat gaaacagtac
      721 gacggctgca tagatatgct gttcgaaccc aataaaaaaa tcggcagatt gctgtatcat
      781 cccggcacgc tgttctggct gctggcgggc tatttcctct gcaacgacgc cgacatagtc
      841 gccatatttc acaaacgctg attttggaga cggttggaac ggctcttgaa agaaagaacc
      901 gccctatttc tccatgcagc aattcacaaa aatcggaacg agtagagcat gttaaacttg
      961 atatgcgtca tttctgctgg ctgcaacatc gctctgctac gtcaggctgc atcggaatac
     1021 gggcagtatt cctgctttcg ccttccttgc atagcttgtt tcgccaacgc ataaagtgcg
     1081 catctcaaat ttaacatgct ctagagtttt tctgaacaaa tccttgattt ttctcaaaaa
     1141 agtaataaaa aaagggcgga tatgcgaaat ccgccctaac acgttgttac acattacaat
     1201 ggtaggtatg tagtgtttcg ttaagcagtc tccttggagc gtttcaaagg cgacagtttg
     1261 tatgtcgggt aactcggcta cagttggcag tggcaggctg tagccgaaat tctgtaaaat
     1321 tatttttgcg gctgcggcaa cggcttgccg tatcgcttct gcatctgcat tttggtaatc
     1381 ttgagggtct gcataatctg tttacgttct ctcggcgtag tggttttcat gccaagcggc
     1441 atggctaaaa cacttcgtga aagaattttc tttttccgtt ccggcgaatc attgccgaac
     1501 atcgcattaa aaacctggta catcctgtcg tagccctgag tcatttcagc aacattaagc
     1561 atggtgttga ttttgacggc ttgttctttg gggtcaagtt ggcagatagg ccgcagccgt
     1621 tcggaaacca tgtttatctg cacaacatcg ccggcatgct gatttttggc ataaagcctt
     1681 ttgagaaaac cacccatttc ttcttcgccg ttactcgatt ttattctgtc cgtcaccctg
     1741 ttaatgtatt ctttccgctc ggtgaccggc attttcaaaa aagcctcgga tgaaatatat
     1801 ctttcgacgt cgttgagttt ggcgatttca ggattcggag cggctggctc gtaagccgat
     1861 attactatca ccgttatggc aacaaccagt accgcgcaga gaacgccggc cattatcttg
     1921 accattttcg gattcgttcg caggtcattt ttaaaagatt cgataagttg ccgatacatt
     1981 gcaaaatctc ctgggctgaa cctgaatcag ccggcagttc atggttactc gggataactt
     2041 aattggcttt ttatggcgtt gaggttatcg gatatatcgt cccatgaagc gtattcaaca
     2101 tgcgagtcgt tgaacatatg attctggccg tccggtttct gcttggaagc gtgggcagct
     2161 tcgccggcgt cgctctgggc atcataaaaa atcggtatct tgtcaggaaa atgctcggcg
     2221 tttatcctga aaaactccgc gccgacatca tccatcaagc cggccatata agtcatgaca
     2281 tactcgtaac ttgagccgta catctggtaa ttctcactgc cgataatcga cggacatttc
     2341 cagaaaacac cctcgcctac ataatccttc atcgccacca tgatattgta attgccgctg
     2401 tctttttcgt agggatacag ttctatcgac tctccatcgg tatctttata cggcatgcag
     2461 cggttgttgg tatccaggta catcgcccat gcattgccga ttcccttgag cgtggcgctg
     2521 catgccacgc gctgcgccaa ctggcgcgcc gtgccgagcg acggcatgat tatgcttacc
     2581 agcaacgcta taatgctgat aacaaccagc agttcgataa gcgtaaaacc tttattgctt
     2641 atatgtgtat ctttcattac aagacccatt tattatcgtg ttatcgccgt ggtttcaaag
     2701 caaagcgact tcttacaatc tgccttaata cgccttcccc ggccttggtt ctatttcaat
     2761 ggccgccgtc tatttttttc gattcgttga cgacctggct catcataaat ttcaattcat
     2821 catttgtcgc aaatcgtata ttcaccgatg cgccatcgga ttttttcaat gtgacgcgcc
     2881 gtatcagatt gctgctgcgg tcgtaaacca gagtcactcg catcgcattg aggatactgt
     2941 cgatgcgcgt gcggtatttg atgaaatcgg cattgtcatc catgcttccg ttgctataca
     3001 gctggcagag agcaacgaag cggaaaagcc tgtcaacctg cctgggcata atcctgagca
     3061 aagccctaag cctggtttcc atcaaaatat gactcacggc gataaaattc tttttagccg
     3121 ccggagtcat cgcagaattt gacgcctcaa taccggcgtc gagaaccagc atattcgcat
     3181 agctggccat catcgcagcc tgcataagcg ccttgatgct gatattctgc ggcttgtcga
     3241 taacaccggt catctcagat ctgttgccgt caatcctggt tatctggaaa tcgcttgcgg
     3301 aatttttgag agagtttata agctcctgaa tatttttgag ttcaattctg ccggtgataa
     3361 aacgtcgcgt tgcggaactg ccggaaatat cgtcgaaacg tatcgcaatt tccctgctac
     3421 tgccgctgtc gcactgattc atatcagcca atacatccac gccgccgaca gaaagtttct
     3481 ttaccagcag cagccccgtg gaattttgcg aaatatacac aatatcagct gaaactgcat
     3541 ctttcccgac gtggccggac aaaggccgta tattcagacc aacatacgcg ttttcgtcgg
     3601 tattgctgca catgaaatcc atatttatga catgcttctg catttgcaag ccgcaaacgc
     3661 tgaatttcac cacctgactg gcggtaatgg tgttgtcgac gctataaaac acttcatcgg
     3721 cagtgaggct gtttcgattc acaaatatgc cgacgagtcc cgccatgacc aacacggcgg
     3781 cagcagcgac gtatgtgccc catactcgcc attttacggc atggctgcgg cgaggagcgg
     3841 ttttgccggc tgatgacgcg atagtctccg cccaacaggt tgtctgccgg acactcggcg
     3901 acggcgaaag cctttcccgc cggccaagct cacccagcac tgcatcccaa agtgcatccg
     3961 aattatcgcg tctgttttcc atgttatttc aaattttcac gaatccaaat ttttcacaat
     4021 tttttacaaa atttcacaaa ttcacaaatc gttgtcagcc agtttcgccc tgagcgtctg
     4081 cctggcccga tacagtcggc cttcgacggc tcgctccgat attccgaccg ccgccgcaac
     4141 ctcactctga gaaaagcctt cgaaatagtg cagccttatt atttcctgat ctatcccggc
     4201 aagcgaggtt actgccgtca tcagtttctg acgcttttcg tcgtcagcca gtatatcttc
     4261 gggccggtcg ccacgttcga cccgctcaac cagttccatt ccgtcgaagg ctgttgccat
     4321 cggtatgttg tttcgcctgc gtaacatgcc gcggatgaaa tttcgccaca cattacgtgc
     4381 cacgccgaac agccatgcat cctgaaaatc atcgtcgccg gccggcaatt ccgttctggc
     4441 gagctggaga cagagttcct gcataaggtc ttccgcattc gattcactac cgctccggtg
     4501 caggaaaaac cgccacaggc ccgtcgcatt gcgtaaaaaa agagtttcga taccgccgcc
     4561 ggctaccgtc ttgggcctgt cggagctttt caaattgtct tttttacgcc acaatgcttt
     4621 cactctttaa atagccgcgg aggcttttgt cccataaaaa ttttaaggct gccggcagca
     4681 taatttgccg gctcaagaca aaaaaacaga cagccgttca taagctgcct gtttttcgaa
     4741 tgttatattc atcatgacga tttcgtcaaa ccgtaaaaca cggatatttc atgttattcc
     4801 gacgcagtca tccgccataa acatgcggct ggcaatgata agccggaatt tttttaacat
     4861 cctgaaagaa tggcaattcc gttcagttcc actgaagttc gccgcctttt acgactttgc
     4921 ctatctggta gcagcgttcg ccgcattcgg aaagctgcgc cataacactt cgggcatatc
     4981 gcggcgaaac aatcactaca taaccgatgc ccatgttgaa tacgcggaac atctccgaca
     5041 tatctacggg gccttttttg gcgatgagtt tgaacacggg cggtatcggc caggaatctc
     5101 gtttaatcct gacggtaagg cctttgggca gaacgcgagg aacgttaccc ggcaggccgc
     5161 cgccggttat atgggccatc gcctttaccg gcatggtcga ttcatagtgg tcaagcagcc
     5221 tgagtatcgg ctgaacgtat attcgcgtgg gcttgaggag ttcttcgccg atgctgcagc
     5281 cttcgagctc ttcgggcttg tcgctcaact tatagccggc tatttccagc aatacccgcc
     5341 tggccaagcc gtaaccgttg gaatgtacgc cattgccggc taagccgatt acaacgtcgc
     5401 cggctttagc cctgccgccg tctataatcc tgctgcgttc caccacgccg acggcaaagc
     5461 cggccatgtc gaattcatcg ggcttgtaga agctgggcat ttcagccgtt tcgccgccga
     5521 taaggctgca attcgactgc ctgcagccgt cggttatgcc gctgactatt tcagccatct
     5581 tttttgggtc gagcctgccg acgccgaggt aatccaggaa aaacaacggc tctgcgccgc
     5641 cgcagatgag gtcgttaacg ttcatggcta ccaggtctat gccgacggta ttcagcacgc
     5701 ccatgccgaa agctatcatc agcttcgtgc ctacgccgtc cgtgcaggca gccaacacag
     5761 ggtccttgta tttcttctta aacagctttt cgccgaagtc cagcctgaac aagccggcga
     5821 aactgccgaa attgtccatc acccgcggcg aaaacgtact tcttaccatc ggtttgatga
     5881 ggtcaaccat ctcgtcgccg gcatcgatat cgacgccagc ctgtttgtat gttatgcttt
     5941 tttctgacat gccgcttatc ctatcaaaag cgcagcccat tgcaacaccg tccaacgcaa
     6001 cgtgagaaat taatttattg aaaacccgtc cgcactgcga cagtgacaac aactttattt
     6061 gcaataaacg cggcaaaaaa ccgcatctta attgactttc actcaaaaaa cagatagaaa
     6121 gacaaaagat tgcaaaggat atataaaaaa catttttccg gagccgggta ctttggccag
     6181 aatcgttttc acaacatttt taagcatcgc ggcagtgatg acgtttgcag ggttaggcgg
     6241 ctgtgatgca cctgttgaga aaaagcaacc ttcgccagcc ggccagacga taactgcaag
     6301 tgaaatagcc gaattcgaca cgctccgtca gcagcttctg cagggcaatc gcacggagca
     6361 gacccgtctt gacgcggcaa gaatactttt gagcaagacg tatccgcagg ctgccgagct
     6421 gctctgccgg ctgctctacg acgccgcccg gccaaccacg cgcgtagccg tcgcgcaggc
     6481 tatttcggaa gaatcatcgc ccagaacaga attttacgac ccgctgatgg atatgctcaa
     6541 aagcgacctg tccaacgttc agcttgccgc cgcacaggca cttgcgagtt tcaatgacca
     6601 caagacggta acttcgctaa tcgaaattgc ggaaaatccc aaaaaaccgc gagaaatgcg
     6661 gctagccgta ataacagcca tgcgaagctg cattcagccc gaatcaatag aatcgctcat
     6721 agaactccta aaggaccacg acaaggtcat acgcaacact gcgacggaat cgcttgagca
     6781 cctcactggc atggaatcat tcggcataaa ctaccgtcga tggcgttcat ggtgggatgc
     6841 ccgcgagttc cagacaaaaa cccaatggct ggaaactctc gatcaaaatc tcatgcgcaa
     6901 ccagatagag cttgagcgta aaaacgccct tcttgcggaa cgtctcactc agacgcttca
     6961 ggatctgttc aattccgccc cgaaaaactc taaaaacgac atgatacaac agatgctggc
     7021 agacgcactg ccggaagttc gtctgctggg cgtcaagctc gccatgaagc tcatagacac
     7081 cggccaaaca ctgccagacc agacagtaag ccggctcatg gctatgatgt acgattcctc
     7141 ggcgcaggtt cggcaggctg cagccacagt tcttgcagac ctgaatcacc cgggctctgc
     7201 caagactctg ctgaactgtc taaaaaccga aacctaccct gaggtgcgcg tagccatact
     7261 tgaagaactc ggccagctga aaaagcccct gactctgccc gcgataatac agagcatcgc
     7321 gtctgaagac gaccaggaat ccgccgccgc cgctcaggcg cttacgcaac tggctcaggc
     7381 tcatctcatc gcgcagccgg cggaaatctc gcaagccaca tccgcactga tagcccgcta
     7441 cgaatcgctg ctgaataacg aaaattcata cgcattgcgc gaagcgttgc ttacggcaat
     7501 gggttcgctg gctgctccgg cggcaaagac ggttctgcag aacgcggtca attccaaatc
     7561 aggcgctata cggctggcag ccatcgcagg gctggaacaa tataaagaca ggtcgctggc
     7621 tcccattttt gcggaaaaaa tccacgattc cgaccgtggc gtacggctgg cggcggtcaa
     7681 ggccctgaca ggcgcaaacg acagaaaata tctcgaaaca ctcataatga tggccagccc
     7741 gaaagtggaa tctgacctgg ctatacggca ggccgccgcg caggctgctc ttgaaatctg
     7801 tcgcaacgcc gacgctggca cgctcgttac cactctcaaa cttctccgac agcaacagca
     7861 gattgattcg tcgttaaaaa tcgaacttct gcagctatat ctaaaatcgc tgacgaatca
     7921 ggaaacgccc agaaaactaa tggtcgaact cgacctcgca gacgaactcg tgctgaactc
     7981 ccagccgaat gacgccgcgg ttcttctgtc gcaggcagtg cggctggcca aaaaactggc
     8041 acaaacgtcg ccggtcagtc cggctgaaat atgggaaaaa tatatccaac tcctcatcga
     8101 cacgggtgcg ccggaaacca taaaagaaat cgcagaccag aatgacccca agcagcgcaa
     8161 ggtggcaata gaacatattt tgagctggat acgttcgccg gcggcggata aaaatctcac
     8221 ggctaccatc acggtaacgg atggaacgct cacaaaactt ggcgaagatt tgactgacgg
     8281 ccagaaaaag gcgctccgcg gcaaaatgct tgccgccgtc aaaacccagc aagttataga
     8341 ccgccagcag gttgcacggc tgctcacgga ctatatgggc acggacggag tcaaatccgc
     8401 cgacgcactc aagcagataa aagctatggg cgacagagcc ttaaaaccac tcctggaatc
     8461 gctcaaaaac gtaatcggcg gcgaaaaggc taaccccgac caggaaaaat cgataataat
     8521 gctaataaaa caggtcgccc cacaagtcgg agattacgac ccgaatgagc cggctgcaca
     8581 aaaaatcaca cgaataaacg actggctgaa aaaactttaa aaattgaaga gaaaaaaata
     8641 taaaggaaga aaggcgttgc tgtcatgaat cgcgtctgta tcggaattga cttcggcggg
     8701 acttttataa aattcggcac actggcagaa gacggcgtaa caacaagtga aatactgcaa
     8761 cttcccacgc cggtaaagga tggcccggcg gctatcatag cacagataaa aaacggcgta
     8821 cggcagatta tcgaacgcga ctctctttcg catgaaaata tcgttgcgct cggcatcggc
     8881 tcgcccgggc cgctgagcgt aaaacgcgga atcatcctca atctgccgaa catacccaac
     8941 atgcagaaca tacccatcgc acggattata ggtgaggagt tcgacattcc ggctgcgctt
     9001 gaaaacgatg caaacgcggc agcctacggc gagtatcttt gcggtgctgg aaaaggctgt
     9061 gaaaatatcg tgatgctcac gctcggcacg ggcgtaggcg gcggagtgat acaggaaaac
     9121 cgcgtcttac acggcacaca tgaaatcggc ggcgagttag ggcacatgat agttcagcca
     9181 ggcggccggc catgcggctg cggccaacgc ggctgtctgg aacaatactg ctcggcggcg
     9241 ttcctggcca aatataccgt ggagaaaata aaaacgcaga atcccgacag ctcgctgact
     9301 aaaacttatc gcgacagcgg cgaacttact gcgctggacg taaacctcgc gcagcaggct
     9361 ggcgacgcat tcgcaataga atgctgggat gaatgctgct actacatggc catcggctgc
     9421 ataaatttgt gcaggatatt cgacccggac agaatcgtaa tcgcgggcgg catgagcaaa
     9481 gccggcgacg aactcataaa tcccacgctc aaatatatcc gcgaattcaa ctggcggatg
     9541 ttcgaaccgc aaacgccgct ggctgtcgcg tcgttaggca acagcgccgg catgataggc
     9601 gcagcaggcg tcgcgtggct gacattcggc gaaaaaactt tcgaataaaa ctttttaaac
     9661 ttgatatacg tcatttttgc tggctgcaac ttcgctctgc tacgtcaggc tgcatcggaa
     9721 tacgggc
//
LOCUS       DAONFI010000045         9715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8003_length_9715_cov_3.682091, whole genome shotgun sequence.
ACCESSION   DAONFI010000045 DAONFI010000000
VERSION     DAONFI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_9715_cov_3.682091"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLK08_02445"
     CDS             complement(<1..308)
                     /locus_tag="PLK08_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPS52388.1"
                     /translation="MATLKDISREVGVSPTAVSRVLNGMPIRIAESKRRQIRQVARSL
                     NYRPNSIAQSLRTSKTKTIGIVVPGLNSAFFPELIQRVELKLSSSGLKAIICDSNDDS
                     G"
     gene            complement(444..1904)
                     /locus_tag="PLK08_02450"
     CDS             complement(444..1904)
                     /locus_tag="PLK08_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HPS52389.1"
                     /translation="MTERFRKSFISRLIPEPQKIIFNGNTCTIDEKMSVTLEISECDD
                     AIRTMVKERFKSYFGVAVDVKIGISHDKITHDGYKLIIEAHYIKISAYGGKGVDYALK
                     TLRQLAEPERNDTTFHHYILPECTIEDFPKMDFRGIHLCIFPETPMWVVEKELRLAAY
                     YKFNYAVIEAWGVFPFRSHPEFCWPDKKKDISEFEHLKGVADECGITLIPQFNILGHA
                     SGSRCGTAKHAIIDFRPELQPLFEPSGWCFCLSNPAVRGILSDIVLELHDFWGHSPFF
                     HVGCDEADGLAVCSECLKHDVKELVKEHICYFHELMAQRKTRIIMWHDMLLNRQDSRW
                     RDCVAFGNEELATLYNELPRDIVIADWQYDLGQKDNQGMEYPTSVFFKKENFDVVMCP
                     WQEAKGTQNIGAMIENHKLFGLLETTWHTAHAFNFFAMFGPAAMVAWRGADFKQRLWW
                     LDMAAHIRQIGYDMNVTSYEQTGCAMNQIMAVTTQV"
     gene            complement(1920..3002)
                     /locus_tag="PLK08_02455"
     CDS             complement(1920..3002)
                     /locus_tag="PLK08_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52390.1"
                     /translation="MMKPIKIAMMSLTHGHTRKYYQTLRDNPKLDWVASCSEDQQSKD
                     VYELYKTGVPCYMSAEEMFRKHPDIEAVVLASANYRHMEQVEQCAERGIHILSMKIPT
                     FDMAEYDRMIKVVDDAGIVCQIELEMHYNPVVKRLKQLAASAEIGKIRSFQATNITLS
                     PVWAFPWQGIPEQSYGRRVPLRVGDARFRGGALCDHPHIFDLIRHLTGSDFEFVYAEV
                     APNIRPDIEVEDMIMLNGKMKDGTAFLFDPSWSRMEERLKVPAAGWEVFPKRMEVNVS
                     ITGEHGTIMCDCFGPNVYHNGAPNDRYTVQYTYFDEWVGLIDEFVDCIRNNKQPLINL
                     RWHRQTIAAMNACYQSIESGKIATVK"
     gene            complement(3002..3700)
                     /locus_tag="PLK08_02460"
     CDS             complement(3002..3700)
                     /locus_tag="PLK08_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52391.1"
                     /translation="MNNKLQMLRNKYDVKDDGVILDGSILKLTGGQCVRLLPWRVERR
                     FIELKKIIEAKTLEDVSTLRFAVMSSSESLKDLLFREFDLCEFLGASRIVRAFAVMNG
                     DDVANILVKLADGKSCSIECSAALPAGTERLDRHEIIARRGVACDRTVDTQVPQSSIY
                     VFGKELETHYTDTDAELFGLCELEILLVRSAMQILQTQALAVEWNAATERIEKIIDKT
                     LQNADRQPAFFEEA"
     gene            complement(3717..4469)
                     /locus_tag="PLK08_02465"
     CDS             complement(3717..4469)
                     /locus_tag="PLK08_02465"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HPS52392.1"
                     /translation="MLNDFGYTDKVFANINRVRDEQSGNIQKAAELMADAISADKLIN
                     VYGGGGHTTLVMGEMFFRAGGLSNINPIMETGISVFNQALKYLELERCVNFGRAVMRY
                     YNLQKDDLLIIFHNIGINAATIDAALEAKEQGVKIIAVSSSYWQNEMPANHFIRHPSR
                     KNLFDLADVCIDDYNPVGDAVVEVPGLATPIAPVSNVVDFTIAHLLEIETIRLCVERG
                     IIPPVWNSANAPGGDEKNAAYLKKYQPRVKSL"
     gene            complement(4481..5509)
                     /locus_tag="PLK08_02470"
     CDS             complement(4481..5509)
                     /locus_tag="PLK08_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52393.1"
                     /translation="MITQTSISYYGLNYVEHAEKDFAEMLNHGCTTVILAITEFDMDF
                     WFPNIPKIVDKAHEMGLRVLLDTWGIGKYFGGEQVSLFLQNNIENRQVSAITDEKLPH
                     ACFNTQSFRDYFQRICLKLAKEVNADGFFWDEPHYAFPKSYASITGGVSDEWTCRCPV
                     CMKKFQDYYGYEMPRVMNDDVKQFRWREALFLLEDTSRKIKAINTDLEITCCVHATLN
                     SYYVTEYRGYDNWDMVASSPYFDVFSTTIIAWDLPETFFEHITRRTVAMAKKYGKQSE
                     RWLMGYYKQPKDFAQIDRVADMYAELGVDRLAAWTYRGGYGTVLAAPDALKLWDRIGE
                     NYLRLLKK"
     gene            complement(5570..6616)
                     /locus_tag="PLK08_02475"
     CDS             complement(5570..6616)
                     /locus_tag="PLK08_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPS52394.1"
                     /translation="MRSSSLKKEQVKKDITYRILSGQYLVNGRIPPERELCDTMAVSR
                     ITVRAAVDDLIAEGILRRDGRRGTIICKIPSTDIAGTKKHGKRLLFLYFSSIRGHVVE
                     QSGTSSRLYHGVERFANEHHYSLMVQSGENFLCQANPDKLEIDGVIVGGTLLEKYIPI
                     LTKAGIPAIVVDSIPHMFDIDAISGDYYEAGARAAELVIRRKLKNPLFIQLKFDDEGF
                     IQPNLLQRQRGFMDTAGREGLDSCVHTIDYRDLNKCGKTVEELFALVKKRHIDGIVYS
                     ADFEYSILSQYKTVGDIYSVVIGGVYAVPPSDKVSQVIFDMEYIGYLAAQRISRRILN
                     PCLEAVRILIPCKQ"
     gene            complement(7031..8242)
                     /locus_tag="PLK08_02480"
     CDS             complement(7031..8242)
                     /locus_tag="PLK08_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52395.1"
                     /translation="MSTKSIFEMAMEQGGGILRLAPAWVPRSFCIPGRRIKLHPDDYY
                     ALGGNRGGIDERWFSSTTPADNGPLTSPNEGLSFVIFQDGGECRKFLLREAAEELKAA
                     LIGERLWNEYHRWPIYSKFFDNQGPLPHHIHHRQQHAKLVGQLGKPEMYYFPTQLNNH
                     GGDFPYTFFGFQPGTQPEQVKQCLMNFTKGDNKITNLSQAYRLEVGTGWDVPPGVMHA
                     PGSLCTYEPQFASDVFAMYQSLVNKQIIPEELLWKDTPKDRVGDFDYLMDVIDWDANV
                     DPNFAENRFMRPVPVRPLDEMVGYRENWICYKSPAVSAKELTVLPGQSVTITDQAAYG
                     MVMMQGHGKMGNWNVETPALIRFGQLTNDEFLISESAAKSGVKITNYSDSDSLVMLKH
                     FGPKNPELVLS"
     gene            complement(8259..>9715)
                     /locus_tag="PLK08_02485"
     CDS             complement(8259..>9715)
                     /locus_tag="PLK08_02485"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPS52396.1"
                     /translation="WLAEFAQAITEHIKKVKPGITVTHQFSPVLAGWLLGQSAGIAAA
                     SDYPSGDFYGGKNQHRLGVKVFNAFRHNDMPLEFMTSRCVDLWDHTSMKSEEELFCSA
                     VTTLAHGGAYFFIDAINPDGTLEPSVYERLGKVSALAKPFTDIIKKLRPEIQAEVGLY
                     FSMESNDDARLNGTPLIEFSPKANNMEGERFVPVIKEILGTAVVLGECNIPYKIVTPE
                     SKLDGLSTIIVNNAKYMSVDEVQRLRRFVYGGGTLIVTGRTSFCDMLGNSKGDFALAD
                     VLGVSYAGRKTPRISYLHDENGHIVSTEPAPLVHATAAKSLAKVSLPYFDPDDTEHYA
                     SIHSNPPGDVTEYDALTINNFGSGRCIYLYSSLLGIQHDVHQKFGKKLFGEYIRQDVI
                     DSDAPGCVAITLLSSKVDDKKLLLCFVNWQEELPNIPVHNISATITLPGNAVCSNCCS
                     VSGTNEVDWRIDGGRLTVKIPRLETAEFIEITLK"
BASE COUNT         2704 a         2390 c         1932 g         2689 t
ORIGIN      
        1 cctgaatcat cattactatc gcaaataata gccttcaggc ctgatgatga aagttttaac
       61 tcgacccgct gaataagctc cggaaaaaat gccgaattca agcccggtac aacgatgcct
      121 atggtttttg ttttactggt gcgtagactt tgggctatcg aattaggcct ataattcaga
      181 cttcttgcaa cctggcgaat ttgtcgcctc ttcgattcag caatgcgtat gggcataccg
      241 tttaacaccc tgcttactgc tgtgggcgaa acgccaactt ctctggaaat atctttaagt
      301 gtagccatct agttttctgt ggtaaaaggt tatatcatat ttggtatatc gttgtatcat
      361 atggtatcat aacttctttt attgtcaata tctaatttaa taaaattaag acgaccagag
      421 tggttttccg agaaaaagct gcattaaacc tgagttgtaa ctgccataat ctgattcatg
      481 gcacatccgg tttgttcata tgaagttacg ttcatgtcat agccgatttg ccgtatatgg
      541 gcagccatat ctagccacca cagacgttgt ttaaagtcag ctccacgcca cgctaccatc
      601 gcagcaggcc caaacatggc aaaaaaattg aaagcgtgag ctgtatgcca agtggtttcc
      661 aacaaaccaa acagcttgtg attttcaatc atggccccaa tattctgcgt acccttagcc
      721 tcctgccacg gacacatcac gacatcgaaa ttctctttct tgaagaaaac tgaagtcgga
      781 tactccatgc cctgattatc tttttgtccc aaatcatact gccaatcggc aatgacaata
      841 tcgcgaggca attcgttata aagagtagca agttcttcat tcccaaaggc aacacaatca
      901 cgccatctcg aatcctgccg gttcagcaac atgtcatgcc acatgatgat tcgcgttttg
      961 cgctgcgcca taagttcatg aaaatagcag atatgttcct tgaccaattc tttaacgtcg
     1021 tgtttcagac attcgctgca gacggccaaa ccgtcagctt cgtcgcaacc gacatggaag
     1081 aatggagaat gtccccaaaa atcatggagt tccagaacta tatcggacaa tatcccccgg
     1141 actgccggat tgctcaagca gaaacaccag ccggaaggct caaaaagtgg ttgcaattca
     1201 ggacgaaaat caattattgc atgtttggca gtaccacaac gtgacccgct ggcatggcct
     1261 agaatattga actgaggaat aagtgttatt ccacattcat ccgcaacacc ctttaaatgt
     1321 tcaaattcag aaatatcttt tttcttatca ggccaacaaa attcaggatg cgacctgaac
     1381 ggaaaaacac cccatgcctc aataacggca taattgaatt tataatatgc cgccaaccga
     1441 agttcttttt ccacaaccca cattggagtt tcagggaata tacacaggtg aatgccacga
     1501 aaatccattt tgggaaaatc ttctattgtg cactctggca atatataatg atgaaatgtt
     1561 gtatcatttc gttcaggctc tgccaactgc cgcagtgttt tcaaggcata atcgacaccc
     1621 ttgccgccat atgccgaaat ttttatatag tgcgcttcta ttattaactt ataaccatca
     1681 tgcgttattt tatcatgact aatgcctatt ttaacatcga ctgctacgcc aaagtatgat
     1741 ttgaagcgtt cctttaccat tgttcggata gcatcgtcgc attcggaaat ctcaagcgta
     1801 acagacatct tttcatcaat ggtacaggta tttccattaa aaataatttt ttgaggttca
     1861 ggtataagtc gggatataaa tgattttctg aaacgctctg tcattttaaa attcctttat
     1921 tattttactg tagcaatttt cccggattca atactttgat agcatgcatt catagcggca
     1981 atggtctggc gatgccatcg cagattaata agcggctgtt tgttgttccg gatgcaatcg
     2041 acaaattcgt caatcaagcc aacccattcg tcaaaatagg tatattgcac cgtataacgg
     2101 tcgttaggcg caccattatg atagacgttc gggccgaaac agtcacacat tattgtaccg
     2161 tgttcgcctg taatggacac atttacttcc attcgctttg gaaaaacttc ccagccggcg
     2221 gcaggaactt tcagacgctc ttccatccgc gaccatgacg gatcgaacag aaacgccgta
     2281 ccatccttca tctttccgtt cagcataatc atgtcttcaa cttcaatatc gggccgaata
     2341 ttcggcgcaa cttcggcata gacgaactca aaatcgctgc cggtcaagtg tcgaataagg
     2401 tcgaaaatat gtggatggtc acacaacgca ccgccgcgaa aacgtgcatc tccgactcgc
     2461 aacggaacgc gacggccata actctgctca ggaatgccct gccaaggaaa ggcccacaca
     2521 ggcgataacg taatgtttgt agcctggaac gaacggattt ttccgatttc ggccgatgcc
     2581 gccaattgct tcaaacgttt gacgaccgga ttgtaatgca tttccaattc aatctgacag
     2641 acgataccgg cgtcatctac aacttttatc atgcggtcat attcagccat atcgaaagtc
     2701 ggaattttca tagacagtat atgaattcct cgttctgcgc attgttcaac ctgctccatg
     2761 tgacgatagt ttgcagacgc caacacgact gcttcaatat caggatgttt cctgaacatt
     2821 tcttccgcac tcatataaca cggcacacct gttttgtaca gctcgtaaac atctttcgac
     2881 tgctggtctt ccgaacagga cgccacccag tcgagcttgg gattgtcccg taaggtctgg
     2941 taatatttcc gtgtatggcc atgcgttaac gacatcatgg caatcttaat tggtttcatc
     3001 atcaagcctc ctcaaaaaac gcaggctgtc tgtcggcgtt ttgaagcgtt ttatcaataa
     3061 ttttctcaat tcgttcagta gcggcgttcc attccaccgc caatgcctgt gtttgaagaa
     3121 tttgcattgc actgcgaacc agcagaattt ccaattcaca aaggccgaat aattcggcat
     3181 cggtatcggt gtaatgcgtt tccagctctt tgccgaatac ataaatcgac gactgtggca
     3241 cctgcgtatc gactgtgcgg tcacaggcta caccgcgtcg ggcaataatc tcatgcctgt
     3301 ctaatcgttc agtccccgcc ggcaacgcag ccgaacactc aatgctgcaa ctcttaccat
     3361 cggcaagttt aactaaaata ttggcaacgt catctccatt cattacggca aaagccctga
     3421 cgatgcgcga tgcgcccaaa aactcacata aatcaaactc acggaataac aaatccttca
     3481 atgattcact cgaactcata acggcaaacc gcagagtaga aacatcttcc aacgtctttg
     3541 cctctatgat ttttttcagt tcgatgaaac gacgttccac tcgccacggc agtaatctga
     3601 cgcattggcc acctgtcaac ttcaggatac ttccgtctaa tatgacgcca tcatctttaa
     3661 cgtcatattt attacgcaac atctgaagtt tattattcat gattctcata ttcattttaa
     3721 agactcttta ctctgggttg atatttttta agatatgcgg catttttttc atcgccgccc
     3781 ggagcgttag cgctgttcca gacaggcgga attattccgc gttcgacgca aagtcgtatc
     3841 gtttctatct ccaacaggtg tgcgatggta aaatcgacca cgttcgagac tggggcaatg
     3901 ggcgttgcaa gtccgggcac ttcaacgaca gcgtcgccga caggattgta atcgtcgatg
     3961 cagacatccg ccaaatcaaa caggtttttc ctggaaggat gacgaataaa atgatttgcc
     4021 ggcatttcat tctgccagta tgaactcgac actgcaataa ttttaacgcc ttgctcctta
     4081 gcctccaggg ctgcatcaat cgtagcagcg ttaatgccta tattgtggaa aattatcagc
     4141 aggtcgtctt tctgcaggtt atagtatctc attactgctc tgccaaagtt cacacagcgt
     4201 tccaactcca gatacttcaa tgcctgattg aaaacggaaa tgccggtttc cataatcgga
     4261 ttgatgttac tcaagccgcc ggcacggaaa aacatttcac ccatcaccag cgtggtatgc
     4321 ccgccgccac cgtaaacatt gattagtttg tcggcagata tggcatcagc catcagttct
     4381 gcggctttct gaatattacc cgactgttcg tcacgtacgc gattgatgtt cgcaaaaacc
     4441 ttgtcagtgt aaccaaaatc atttaacata atttattcct ttatttcttc aataaacgta
     4501 gataattttc accaattctg tcccacagtt tcaacgcatc aggagcagct agcacagttc
     4561 cgtatccacc gcgataagtc caggcagcca gacggtctac gccaagctcc gcatacatgt
     4621 ccgcgactct gtcaatctgg gcaaaatctt tcggctgctt gtagtagccc atcagccatc
     4681 gttctgactg cttaccgtat tttttagcca ttgccaccgt acgtcgcgtt atatgctcaa
     4741 aaaacgtttc aggcaaatcc cacgcgataa tcgtcgttga aaagacgtcg aaatacggac
     4801 ttgacgccac catatcccag ttatcgtaac cgcgatattc cgtcacataa taactgttga
     4861 gcgtagcatg aacgcagcag gtaatttcca aatcagtatt gatagccttt attttccgcg
     4921 atgtatcctc aagaagaaac aacgcttcac gccagcggaa ctgtttgacg tcatcgttca
     4981 ttaccctggg catttcgtaa ccgtagtaat cctgaaattt tttcatgcag acagggcagc
     5041 ggcacgtcca ttcgtcggat acgccaccgg taatagacgc ataagacttt ggaaatgcgt
     5101 aatgcggttc atcccagaag aaaccgtcgg cattgacttc tttcgcaagt ttcagacaga
     5161 tgcgttgaaa ataatcacgg aatgactgcg tattgaaaca cgcatgaggc agcttttcgt
     5221 cggttatagc tgaaacctgt ctattttcga tattgttctg caaaaacagg cttacctgct
     5281 caccgccgaa atatttgcct atgccccatg tgtccaaaag tacccgtaat cccatttcat
     5341 gggctttatc cacaatcttg ggaatattgg gaaaccagaa atccatgtcg aattccgtaa
     5401 ttgcaagaat taccgtcgta caaccatgat tcagcatttc ggcaaaatct ttttccgcat
     5461 gctccacata attcaggccg taataactga tagatgtctg cgtaatcatt cttaaaaatc
     5521 cttataggta aatttataaa tgtgatagca aaaacttttt atcgcaatat cattgtttac
     5581 agggaattaa aatacgaacc gcttccaggc atggattcag aatccgtcgc gatattcgtt
     5641 gagccgccag atatcctata tactccatat caaaaatgac ttgtgaaact ttgtcagaag
     5701 gcgggacagc atataccccg ccaatcacaa ccgaatatat atcgccaaca gttttatatt
     5761 gagataatat ggaatattca aaatcggcac tgtaaacaat tccgtctata tgccgttttt
     5821 taacaagagc aaataattct tcgacggttt ttccgcattt attcaaatcg cggtaatcaa
     5881 tagtatggac acaactatcc agaccttctc ttcctgcggt atccataaaa ccccgctgac
     5941 gctgcaaaag attcggctga ataaaacctt catcgtcaaa tttaagttgt ataaaaagcg
     6001 gattcttcaa ttttctacga ataacaagtt ctgcagcccg agcgccggct tcgtaatagt
     6061 cgccactgat agcgtcgata tcaaacatat gtggaatgga atcaacaaca atagcaggaa
     6121 tgcccgcttt tgttaaaatc ggaatatact tttccagcaa agtcccgcca actattacgc
     6181 catctatctc caatttatca ggattggcct gacataagaa attttcgccg gattgcacca
     6241 tgagcgagta gtgatgctca tttgcaaaac gttcaacccc atgataaagt ctcgaagatg
     6301 taccgctctg ctccactacg tgtccgcgga tacttgaaaa atagagaaac agtaatcttt
     6361 tgccatgttt tttcgtgcca gcgatatcag tcgatggtat tttgcaaata atagtaccac
     6421 gtctgccgtc acgccgtaaa ataccttctg ctattaaatc atcgacagcc gcccgcacag
     6481 ttatccgact aacagccatc gtatcgcaca attcccgctc aggaggaatt ctgccgttaa
     6541 ccagatattg gccggataag atacggtagg tgatatcttt cttgacctgt tcctttttta
     6601 agctactgct tcgcatatta agctcttttg ggttatccta tacagtaaca taataccaaa
     6661 atatgacaaa gtcaagataa atattttccc tatacttgat agcggtcaca gttgatttaa
     6721 atacgtggcc actgcatgta aacggatata gagaacgttt ggaattttgt caagtatcat
     6781 gaggctaagt acaaaagact taaaaaggct ggcgcatctg tgtgtaatgt ggtacacaat
     6841 ggcatatgca agacaaggtt acaaattgcc cttgatatca ataaattcat tcgcttggct
     6901 gaaaatagat tctgaaaatt tagagttttc catgcaaaag tctgtttcta taatttgcca
     6961 tggatctata agttgattaa cagatgtttt tgacataatg gcataccatc cagataatgg
     7021 aaatattatg ttaagatagg acaagctctg gattttttgg gccaaaatgt ttaagcatca
     7081 caagtgaatc ggagtctgaa taattggtta ttttgacacc ggatttagcg gctgattcac
     7141 tgattaaaaa ctcgtcattg gtcaactgtc cgaagcgtat caacgccggc gtttcgacat
     7201 tccagttacc cattttccca tggccctgca tcattaccat accgtaggcg gcttggtcgg
     7261 taatcgttac gctctgaccc ggcagcacgg tcaattcctt tgcagacaca gccggcgact
     7321 tatagcaaat ccaattttcg cggtagccta ccatttcatc taacggccga acgggtacag
     7381 gcctcatgaa acgattttcc gcaaaattcg ggtcgacatt ggcatcccag tctataacgt
     7441 ccatcaaata gtcaaaatcg cccaccctgt ctttcggagt gtctttccac aacaattcct
     7501 caggaataat ctgcttgttc acgagcgact gatacatggc gaacacgtcc gatgcaaact
     7561 gcggttcata cgtacacagg ctgcccggtg cgtgcataac tccaggaggc acatcccagc
     7621 cagtccccac ctccaaccta tacgcctgtg atagattggt tatcttgtta tcgcctttcg
     7681 tgaaattcat caggcattgc ttgacctgct caggctgcgt gccgggctga aaaccgaaaa
     7741 acgtgtacgg aaaatctccg ccgtgattgt tcagttgggt cgggaaataa tacatttccg
     7801 gctttccaag ctggccgacg agtttcgcgt gctgctggcg atgatgaata tggtgcggca
     7861 gcggcccctg gttatcaaaa aactttgaat agataggcca acgatgatat tcgttccaca
     7921 agcgttcacc tatcagagcg gctttgagct cttcagcggc ttcgcgaagc agaaattttc
     7981 tgcattcgcc gccgtcctga aatatgacaa aactcaaacc ttcattgggt gaagtcaatg
     8041 ggccgttgtc ggctggtgtg gtcgaggaaa accaacgttc gtctataccg ccccgattcc
     8101 cccctaatgc gtagtaatcg tcaggatgta atttgattcg ccgccctgga atgcaaaaac
     8161 tacgcggcac ccacgccggt gccaatctga ggataccacc gccctgttcc atagccattt
     8221 caaaaattga ttttgtcgac atcttttaca ctcccaaatt actttaacgt tatttcaata
     8281 aattcagcgg tttcgagcct tggaattttt actgtcaatc tgccgccgtc tatccgccaa
     8341 tcaacctcat tcgttccgga aacactgcaa cagttcgaac ataccgcatt tccaggcaat
     8401 gtaatggtcg ccgaaatatt atggactgga atattcggca attcctcctg ccagttgacg
     8461 aaacacaaca gtaatttttt atcatcaact ttcgagctaa gcagcgttat cgcaacacat
     8521 ccaggcgcgt cggaatcaat aacatcctgc cggatatatt cgccgaaaag tttctttccg
     8581 aatttctggt gcacatcatg ctggatacca agcaaggatg aataaagata aatacatctc
     8641 ccactgccga agttgttaat cgtcaaagcg tcatattctg taacatcgcc aggcgggttg
     8701 ctgtgaatgg acgcgtaatg ttcggtatcg tcgggatcga aatatggcag cgacactttt
     8761 gcgagacttt ttgcagccgt agcatgcacc agcggagcgg gctcggtaga aacgatatga
     8821 ccattttcat cgtgcagata actgattcgt ggcgtcttcc tacccgcgta tgaaacgccc
     8881 aaaacatcgg ccagtgcaaa atcaccttta gaattaccca gcatgtcaca aaatgaagtt
     8941 ctgccggtaa ctataagcgt accgccgcca tatacaaacc gcctcagccg ctgaacttca
     9001 tcaaccgaca tgtattttgc gttgtttaca ataattgtgg acaaaccatc cagcttggat
     9061 tcgggtgtaa caattttata aggaatatta cattcaccaa gtaccaccgc agtacccagt
     9121 atctctttta tgactgggac aaatcgctcc ccttccatat tatttgcctt aggcgaaaat
     9181 tcgataagcg gcgtcccgtt cagacgggca tcgtcattgg attccattga aaaatacagc
     9241 ccgacctccg cttgaatctc aggtcgcaat ttcttaataa tatctgtaaa cggcttagct
     9301 agagcggata ctttcccaag ccgctcgtag acgctcggtt caagcgtacc atcaggattt
     9361 atcgcgtcta taaagaaata cgccccgccg tgcgctaacg tggtaacagc tgagcagaaa
     9421 agttcttctt ctgatttcat cgacgtatga tcccataaat cgacacaacg cgatgtcatg
     9481 aattcaagcg gcatgtcatt atggcgaaat gcattgaaca ctttgacgcc aagcctatgc
     9541 tgattcttcc cgccataaaa atccccagac ggataatcgc tggcggcggc aattccagca
     9601 ctttgcccca acagccagcc cgcaagtacc ggcgaaaact gatgagtgac cgtaatacca
     9661 ggttttactt ttttgatatg ctccgtaatt gcctgtgcaa attccgccag ccatt
//
LOCUS       DAONFI010000046         9484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8285_length_9484_cov_3.877293, whole genome shotgun sequence.
ACCESSION   DAONFI010000046 DAONFI010000000
VERSION     DAONFI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_9484_cov_3.877293"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..3145)
                     /locus_tag="PLK08_02490"
     CDS             complement(86..3145)
                     /locus_tag="PLK08_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPS52397.1"
                     /translation="MNLATLAIKNKTVTMVWTILICVGGIIAYLHLGRLENPTFTIKT
                     ALVSTTYPGASADEVQQQVTDPLEKAIQQMGQIDKIRSLSQRNLSIIYVDIKDEYNHL
                     RLPQVWDELRRKVNDAQSSLPSGAGPSVVNDDFGDVYGVFFALTGEGYDLHDLKEFAD
                     DLRRDILQLVDAGVAKVDMWGVPREIIYIEFKRSVMSQLGVSPEEIASLLKSQDIVAP
                     SGQVTIGPRYIRIEPTGKFNDIETIGDLILRGKTNSDSIVKLKDVIEIKREYEDPRQS
                     IMYYNGQPAIGIGISTVDGGNVITMGKAVKKKIAQIKGLTPIGMQLHIVSYQSDTVDE
                     AVNGFVINLVEAVVIVIALLLVFMGIHTGLLIGIILLLTILSTFILMWLFNIDLQQIS
                     LGALIIALGMLVDNAIVVAEGILVRVQKGEKRLFAAEETVHETAYPLMLATFVAILAF
                     AAISLNQSNVGEYCRSLFYVIAISLGMSWILAVTSTPLLSTMFLKPPAKEIHDPYAGR
                     FYQTYRRFLVWCLHHRIKVCIVMALLLLSSIYGFRFVQKSFFPDSTRPQFFVDIWCPQ
                     GTHINTTAAITKELDEFIRKQPGVTDVGCFIGKGALRFILPYDMEMPNSSYAQLLVNV
                     DDYQKIDACLKAINDKIAKEPFDANIYAHKFHNGPGNKFKIVARFRGPDTGVLRSLAE
                     KAKDIMRADRTPDSRISTRDVRDNWRQKTMVMHPRFLASRARQIGATVSDLRSALQNT
                     YGGTLIGAYREDNDLLGIMARPVESDRKGIDQLQNQLIWCSAVRQFVPLGQLIDGIGM
                     TWDDDILWRWNRQLAIEAQCNATVETANTVYERLRKKIEQIPLPLGYELEWGGDQDSS
                     SKAQAPIAKTFPLCLLAMFLLVLMLFNSLRPPIIIFMCLPLAIIGVTGSLLVLHKSFG
                     FMAILGFLSLSGMLIKNAIILIDQIRIDESKGSSTYNALLMAGVSRIRPVMMASMTTV
                     LGMVPLISDPMFDAMAVTIMGGLTFGTLLTLVFVPVLYSVFYRVHPD"
     gene            complement(3247..4620)
                     /locus_tag="PLK08_02495"
     CDS             complement(3247..4620)
                     /locus_tag="PLK08_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPS52398.1"
                     /translation="MLFSNGRDKTKSLEEPAARPVKTIVLENQNGVIKRTYAGEIDAN
                     LNSELSFRVSGPLVRLLVQPGQSVRKGELLAEIDPRDYKFNVEKLQSELDKLNTQLKI
                     MKIGAREEEITVCRSRLSLAEARENLAKSELDRIAYGLENNVATQSEYDQAKCAYETT
                     KAETVIARKNLHIAEVGSRKEDIEAMLANIQSVKVQLKMAQAALEDTRLTAPFDGVVG
                     KKYVEENDQVKAGQPIIVLQDNSRTEVVIHIPQNEIETTSTKRKMAVRFTQAGAELSK
                     LSFPLTFKEIDTVADQATKTYRVTLVMVTPKDVPIYPGMTVEVDVESRYNGMPSGFLV
                     PASAIVGRADGQPIVWRINPKTSTAEAVPVTPGQIIGDEIQITGKLKAGDRIIIAGAS
                     FIYNGQKVSASAYRYDEDVRARMRRLHHNSSILPAGTKNITFSSYTPAVTPFARSDNP
                     QSSDIPQ"
     gene            complement(4746..5363)
                     /locus_tag="PLK08_02500"
     CDS             complement(4746..5363)
                     /locus_tag="PLK08_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPS52399.1"
                     /translation="MRTINIDGQSVDQEKIRPLILEACIEVLNVYGQNGLTMSRIARQ
                     AGIAKGTLYNYFSNKEELLKSVCELFFKPIFDQILMMLDAKESPRKILITMALAEVAT
                     FRQYRRVIEAFLLQFGKNSLVDDYASDSFKGFVMELVKRIFELAQTTGADLPFPPKIM
                     AELYDGAAMGLARCIPKSSAEDTNAFPCNEELERFVDIFLPRSRH"
     gene            complement(5584..6459)
                     /locus_tag="PLK08_02505"
     CDS             complement(5584..6459)
                     /locus_tag="PLK08_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPS52400.1"
                     /translation="MKPDNRQYKFRKPPISPAPRFKKPPLAMQVTTLWDYPSQHYGDS
                     LQGDSNYIGATPSYIIWNLLQRYTTQGQLVVDPCCGSGTTIDVSRDIGRRCLGYDVNP
                     TRNDIFNADARKLPLENSAADFVFIDPPYSTHIHYSDDPRCIGKLDAASGAYYKAMEK
                     VIGEINRILRPGRYMAMYVCDSLVKGKRFYPIGFELFGMMRKFFTPVDVIAVVRHNKT
                     LEMGNYRKAADENNFYLRGFNYLFIMYKPLPPKSSTADSHAARKYHNSRKKPSDITKI
                     PAKNSGRKHRDFPAR"
     gene            complement(6525..8777)
                     /gene="pflB"
                     /locus_tag="PLK08_02510"
     CDS             complement(6525..8777)
                     /gene="pflB"
                     /locus_tag="PLK08_02510"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HPS52401.1"
                     /translation="MTRQLGERFEKAWAGFNAGQWQNAVDVRGFIQLNYTPYDGGPEF
                     LASPTPRTQKIMAKYEDLLKQEAARGGVLDVDASTVSSLLTYNAGYLDKDNEIIVGLQ
                     TDAPLKRGLNPFGGMRMAKQACEAYGYHVGEDVQKSFEFRTTHNDGVFHVYTDEIRAA
                     RKSGIITGLPDAYGRGRIIGDYRRVALYGLDFLIEQKRLDKKNIGLQDMSEDNIRLSE
                     ELWKQIDFLEKLKRMAEMYGVDISGPAADAKQAVQWTYFAYLGAIKEQNGAAMSLGRV
                     GTFLDIYFQRDIERGVLTEQSAQEIIDDFVIKLRMARQLRTPEYNELFAGDPLWITES
                     VGGMGLDGRTMVTRTSYRFLHTLRNLRPAPEPNLTILWSSGLPEPFKRYCAELSIDTD
                     SIQYENDDIMRERYGDDYGIACCVSAMKIGRQMQFFGARCNMPKVLLMALNGGRDELT
                     GDIIGPEMDVYDGDVLDYDEVCRRFAFYRDWLCRLYVNTMNIIHYMHDKYAYEKLEMA
                     LHDTEVERFMAFGIAGLSVVADSLSAIKYASVKPIRNADGLITEFETTGEFPLYGNDD
                     PRVDKLAASLVEEFIAALRRHKTYRNAIHTLSILTITSNVVYGKKTGSTPDGRKYGQP
                     FAPGANPMHERDRCGALASLNSVAGISYDACRDGISNTFTITPSAAGKDKPARISNLV
                     DILDGYFCQAGHHLNVNVLDREKLIEAMNHPEKYPQLTIRISGYAVNFHKLSKLQQQE
                     VISRTFHSSI"
     regulatory      complement(8915..9013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
BASE COUNT         2177 a         2273 c         2474 g         2560 t
ORIGIN      
        1 cggcgtaagt taagattgtg ttaacatcca cgcttatttt caagaaccac tataacggcc
       61 ttttactgtt attttacaaa ccgttctaat ccggatgaac tcgataaaat accgaataaa
      121 gtaccggcac gaaaaccagc gtcagcaatg tgccgaatgt caggccgccc atgattgtaa
      181 cagccattgc atcgaacata gggtcgctta taagcggtac catgccgagt accgttgtca
      241 tcgatgccat cattaccggc cgaatacggc tgacgccggc catgagtagg gcgttgtagg
      301 tgcttgagcc tttcgattca tctattcgta tctggtctat cagaattatg gcatttttga
      361 ttaacatgcc ggaaagcgac agaaaaccca atatcgccat aaagccgaaa gatttatgca
      421 acaccagcag actgcctgtg acgccgataa tggccagcgg caggcacatg aaaatgatta
      481 tcggcggccg tagagaatta aacagcatca gaaccagcag aaacattgcc agcagacata
      541 acggaaacgt ctttgctatt ggcgcctgag ccttcgaaga actgtcctga tcgccgcccc
      601 attccagttc atagcccagc ggcaggggta tctgttcgat tttctttcgc agacgctcat
      661 aaactgtatt ggcggtttct accgttgcat tacattgtgc ctctatggct aattggcgat
      721 tccaccgcca taaaatatcg tcatcccacg tcatgccgat gccgtcgata agctggccta
      781 acgggacgaa ctgcctgacg gctgaacacc agatgagctg attctgcaac tggtcaatgc
      841 cttttctgtc agattcaacc ggccgcgcca tgatgccgag caaatcattg tcttccctgt
      901 acgctcctat aagcgtgccg ccgtatgtgt tttgcagggc ggaacgcaaa tcggagacag
      961 tcgcgccaat ctgtctggcg cgcgacgcga gaaaccgcgg atgcattacc atggttttct
     1021 gtcgccagtt gtcacgcacg tcgcgcgtgc ttatgcggct gtcaggcgtt cggtcggcac
     1081 gcatgatgtc tttggccttt tccgccagcg accggaggac gcctgtatca gggccgcgaa
     1141 atcgggcgac tatcttgaac ttgttgccgg gcccgttatg aaatttgtgg gcataaatgt
     1201 ttgcatcgaa cggctctttg gcgattttgt cgtttatggc tttcaggcat gcgtcgattt
     1261 tctgataatc gtccacgttg acgagcagtt gcgcataact gctgttgggc atttccatgt
     1321 catagggcaa tatgaatcgt agcgcacctt tgccgataaa gcagccgacg tcggttacgc
     1381 cgggctgttt ccggataaat tcgtcaagtt ctttggtgat ggcggctgtc gtgttgatat
     1441 gcgtaccttg cggacaccag atatccacaa aaaactgcgg ccgcgttgag tcggggaaaa
     1501 aacttttctg aacgaaacgg aatccgtata ttgacgatag cagcagcagg gccatcacta
     1561 tacatacttt tatgcgatga tgcaggcacc agaccagaaa gcgtctgtaa gtctgataaa
     1621 aacggccggc gtaggggtcg tgaatttctt ttgccggcgg tttaaggaac atcgtcgaga
     1681 gcagtggcgt ggatgtgacc gcaagtatcc aactcatgcc cagtgatatg gcgatgacgt
     1741 aaaagaggct gcggcagtat tcgccgacgt tgctctggtt caggctgatg gctgcgaatg
     1801 cgagtatcgc aacaaacgtt gccaacatga gcggatacgc cgtttcatgc acggtttctt
     1861 ctgcggcgaa cagacgtttt tcgcctttct gaacccgcac cagtatgcct tccgccacga
     1921 ctatggcgtt atcgacaagc atgcccaggg cgataatcag tgcaccgagg gatatctgtt
     1981 gcaaatcgat gttgaacagc cacatcagta tgaacgtact cagaatcgtc agcagcagta
     2041 ttatgccgat aagtaggccg gtgtgtatgc ccatgaagac cagcagcagg gcgatgacga
     2101 taacgacggc ttccacgaga ttgattacga agccgttgac ggcttcatcg acggtgtcgg
     2161 actgatagga tactatgtgc agttgcatgc cgattggagt caggcccttg atttgtgcga
     2221 tttttttctt gacggctttg cccattgtga tgacgttgcc gccgtcgact gtcgaaatgc
     2281 cgatgccgat agccggctgg ccgttgtagt acattatcga ctgccgcggg tcttcatatt
     2341 cacgcttgat ttcgattacg tctttgagtt tgactattga atccgaattc gtcttgccgc
     2401 gaagtatcag gtcgccgata gtttctatgt cgttgaattt gccggtcggc tcgatacgta
     2461 tgtaacgcgg gcctatcgta acctggccgg acggggcgac gatatcctgc gacttcagca
     2521 gggatgctat ctcttcggga gaaacgccaa gttgggacat gaccgaacgc ttgaattcaa
     2581 tatatatgat ttcccgcggc acgccccaca tatcgacttt tgctacgccg gcgtcgacaa
     2641 gctgcaatat gtcgcggcgc aggtcgtcgg cgaattcctt gaggtcgtgc aggtcgtagc
     2701 cttcgccggt aagtgcgaaa aatacgccgt aaacgtcgcc gaaatcgtca ttgaccacgc
     2761 tggggccggc gccgcttgga agcgaactct gggcgtcgtt cacttttcga cgcagttcat
     2821 cccatacctg gggcagtcgt agatggttgt attcgtcctt gatgtcaacg taaattatgg
     2881 aaagattccg ctgggacagc gagcggattt tatctatttg tcccatctgc tgaatggctt
     2941 tttcgagagg gtcggtaacc tgctgctgta cttcgtctgc gcttgcgcct ggatatgtgg
     3001 tggaaacgag ggctgtcttt atggtgaacg tagggttttc aagccgccca agatgcaagt
     3061 acgcaattat gccgccgacg catattaaaa tcgtccatac catcgtgacg gtcttgtttt
     3121 tgattgcaag tgtagccaga ttcataacgg gtcgtcttcc aaaatcaaaa tcgttttaac
     3181 tgaattccac ggattttatt taatccgtga aattacaaag gatatataaa aaaatgtacg
     3241 gaaatattat tgcggtatat ccgaagattg tgggttatcg ctgcgtgcga aaggcgtaac
     3301 tgccggcgta taacttgaaa acgttatatt tttagtacca gccggcagaa tgctggaatt
     3361 gtgatgcagc cgccgcattc gtgcccggac gtcctcgtca tatcggtatg cggaggcgga
     3421 aactttttgg ccattgtaga taaaactcgc gccggcgatg attatccggt cgccggcttt
     3481 gagtttgccg gttatctgta tttcgtcgcc gataatctgg ccgggggtta cggggacagc
     3541 ttcggcggta ctggtcttcg gatttattcg ccagacaatc ggctggccgt cagcgcggcc
     3601 gacaatcgcg ctcgccggca caagaaaacc cgacggcatg ccgttataac gcgattcgac
     3661 gtcaacttcc accgtcatgc ctggatatat cggcacatct ttgggcgtta ccataaccag
     3721 ggtgacgcga tatgttttgg tggcttggtc ggcgacggtg tcgatttcct tgaacgtaag
     3781 cgggaagctc agcttggaga gttccgcgcc agcctgcgtg aagcggacag ccattttacg
     3841 ttttgtggac gttgtttcta tctcgttctg cgggatatgg atgacaactt cggtacgcga
     3901 gttgtcctgc aatacgataa tcggctggcc agccttgacc tggtcgtttt cttcgacgta
     3961 tttcttgcct acaacgccgt caaacggcgc cgtcagcctg gtatcttcaa gtgccgcctg
     4021 cgccattttc agttgcactt tcacagactg gatattggca agcatggcct ctatatcttc
     4081 ctttcgcgaa ccgacttcgg ctatgtgaag gttttttcgg gcgattaccg tttcagcttt
     4141 ggtggtctcg tatgcacatt tggcctggtc gtattccgat tgggttgcga cgttattttc
     4201 aaggccatag gcgatacggt cgagttcgga tttcgcaaga ttttcgcgag cttctgcgag
     4261 gctgagtctt gaacggcaga cggttatttc ttcctcacgt gcgcctattt tcattatttt
     4321 gagttgagta ttgagcttat cgagttccga ctgcagcttt tcaacgttga acttgtaatc
     4381 gcgcgggtcg atttccgcca gcagttcacc tttgcggacg gattggccgg gctgaacgag
     4441 cagccggaca agtgggccag atacgcggaa ggagagttct gaattcaggt ttgcgtctat
     4501 ttcgccggcg tacgtccgtt tgattacgcc gttctgattt tccaggacga tagttttaac
     4561 cggccgggca gccggctctt ctagagattt cgtcttatcc ctgccgtttg aaaacagcat
     4621 tacgccgcca aataatatga caatgccggc tatcgaaatc ggtattgcat atcttttaat
     4681 aaattttata actctatttt gtatcaaaac ttcaaacatc ttatatatcc ctttacgcca
     4741 caaaatcaat ggcgtgaccg tggcagaaaa atatcgacaa atctttccag ttcttcattg
     4801 caggggaagg cgtttgtatc ctctgcggag cttttgggga tgcagcgggc cagccccata
     4861 gctgcgccgt cgtaaagttc agccataatt ttgggcggaa acggcaaatc cgcaccggtg
     4921 gtttgagcca gctcaaatat acgttttacc aattccatga caaagccttt gaaggaatcg
     4981 ctggcgtaat cgtcaacgag gctgtttttg ccgaattgca acagaaacgc ctctattacg
     5041 cggcggtact gccggaatgt cgccacctcc gccagagcca tggtgatgag tattttacgc
     5101 ggcgattctt tcgcatcaag catcataagt atctggtcaa aaatgggctt gaagaataat
     5161 tcgcaaacgc ttttcagcag ctcttccttg ttggaaaaat agttatacag cgtgcctttc
     5221 gctattcctg cctggcgggc aatgcgggac atggtcaagc cattctggcc atatacgttc
     5281 aatacctcaa tacatgcctc aagtatcagg ggcctgattt tttcctggtc aacagattgt
     5341 ccatctatat ttatggttcg cataattcgt ttttcacctg aaatgcggag ggctatcaat
     5401 tattatgacc cctcagtcat tattatagtg accgcagggt cataaaatca aataaaattt
     5461 ttgaataatt atcataaaag tgcaagctcc ggattttgga ttataactga tgattttata
     5521 aagagatacc acaggggaca aagtatggat ttttgacgat tgcggctggg ctgtgcacaa
     5581 actttatctg gcggggaaat cacgatgttt tctgccggag tttttggcgg gaatttttgt
     5641 tatgtccgaa ggttttttcc ggctgttgtg atatttgcgt gcagcatggc tgtcggcggt
     5701 cgaagatttt ggcggcaaag gtttgtacat gatgaaaagg tagttgaaac cgcggagata
     5761 gaaattattt tcatcggcgg ctttgcggta gttgcccatt tcgagggttt tgttgtgacg
     5821 cacgacggcg atgacgtcga ctggcgtaaa aaatttccgc atcatgccga aaagttcaaa
     5881 gcctatcgga taaaaccgtt tccccttgac gagcgaatcg cagacgtaca tcgccatgta
     5941 acggcctggc cgcaaaattc gatttatttc gccgatgact ttttccatcg ctttgtaata
     6001 agcaccgctg gcggcgtcga gtttgccgat gcaccgtggg tcgtcggaat aatgtatgtg
     6061 ggtcgagtag ggcgggtcga tgaacacgaa atcggcggcg gagttttcca gcggcagttt
     6121 acgtgcatcg gcattgaaaa tgtcgtttcg cgtaggattg acgtcatagc ccagacagcg
     6181 gcggccgatg tcgcgggaaa cgtcaatggt cgtaccgctg ccgcagcagg ggtcgacgac
     6241 aagctggccc tgcgtggtgt aacgctgaag cagattccag ataatgtagc tgggagttgc
     6301 gcctatgtaa ttcgaatcgc cctggagcga atcgccatag tgttgcgagg ggtaatccca
     6361 caacgtggtg acctgcatgg ccagcggcgg ttttttgaat cgcggcgccg gcgaaattgg
     6421 cggcttcctg aatttatatt gcctgttatc cggtttcatg cgactaatcg taccgtggcg
     6481 aatcgaattt gccaacaaat tgttttattc agcagtcgca cctctcagat ggaactgtgg
     6541 aatgtgcggc tgatgacttc ctgctgctga agtttggaca gcttgtgaaa attcactgca
     6601 taaccggata tgcgtattgt aagttgcgga tatttttccg ggtgattcat tgcctcaatc
     6661 aatttttcac ggtcgaggac gttgacgttc aggtgatggc cagcctggca gaaatatccg
     6721 tctaaaatat cgaccaggtt tgaaatcctg gcgggcttat ccttgcctgc tgctgatggc
     6781 gttatggtga acgtgttgct tatgccgtcg cggcaagcgt cgtatgaaat gccggcgacg
     6841 gaattcagtg acgccagtgc gccgcacctg tcgcgctcgt gcattggatt agctcctgga
     6901 gcgaacggtt ggccgtattt ccttccgtcc ggcgtagagc cggtcttttt gccgtaaacg
     6961 acattggacg taatggtcag tattgacaac gtgtgtattg cgttgcgata ggttttgtga
     7021 cgtctcagtg cggcgatgaa ttcctcgaca aggctggccg caagtttgtc gacgcgcggg
     7081 tcgtcattgc cgtaaagcgg gaattcgcct gtcgtttcaa attcggtaat caggccgtca
     7141 gcgttgcgaa tcggcttgac ggaggcgtat tttatcgcac tgagcgagtc tgccaccaca
     7201 cttaggccgg ctatgccgaa agccataaac cgctcgactt ccgtatcgtg caacgccatc
     7261 tcaagttttt cataggcata tttgtcgtgc atgtagtgta tgatgttcat cgtgtttacg
     7321 tacagcctgc acagccagtc acgataaaat gcgaatctgc ggcagacttc gtcataatcc
     7381 agcacatcgc cgtcatagac gtccatttcc gggccgatga tatcgcccgt cagttcgtcg
     7441 cggccgccgt tgagggccat cagcagaact ttgggcatat tgcatcgtgc gccgaaaaac
     7501 tgcatctgtc ggccgatttt catcgccgag acgcaacacg ctatgccgta atcgtcgccg
     7561 taacgctcgc gcattatgtc gtcgttttcg tattgtatgg agtcggtatc tatgctcagc
     7621 tccgcgcaat aacgtttaaa aggttcgggc aatccggacg accacaatat cgtcagattc
     7681 ggttcaggcg cagggcggag gtttctgaga gtatgcagga aacggtagct cgtacgggtc
     7741 accattgtgc ggccgtccag gcccatgccg ccgactgatt cggttatcca caggggatcg
     7801 ccggcgaaga gttcattgta ttccggcgta cggagctggc gtgccatgcg gagttttatc
     7861 acgaaatcgt cgataatttc ctgtgccgac tgctccgtaa gtacgccgcg ttcgatatct
     7921 cgctgaaaat atatatccag aaacgttcct acgcggccca gcgacatagc cgcgccgttc
     7981 tgttccttta tcgcgccgag atatgcgaaa tacgtccact gtaccgcctg ttttgcgtcg
     8041 gcagccgggc cggagatgtc tacgccgtac atctcagcca tgcgtttgag tttttcgaga
     8101 aaatctatct gcttccagag ttcttcgctc aggcggatat tatcttcgct catgtcctga
     8161 aggccgatgt tttttttatc cagccgtttc tgttcgatga gaaaatcgag gccgtagagt
     8221 gcgacgcggc ggtaatcgcc gatgattcgg ccgcggccat atgcgtccgg caggccggtt
     8281 attatgccgc tcttgcgtgc agcccgaatc tcatccgtgt aaacgtgaaa cacgccgtcg
     8341 ttatgcgtcg tccgaaattc aaagctcttc tgtacgtctt cgccgacgtg ataaccgtac
     8401 gcctcgcagg cctgcttggc catacgcatg ccgccgaacg gatttaatcc gcgtttaaga
     8461 ggtgcgtcgg tctggaggcc tacgattatt tcgttgtctt tgtcgagata accggcgtta
     8521 taagtcagaa gagatgaaac cgtggaggca tcgacatcga gtacgccgcc gcgggctgct
     8581 tcctgtttga gcaggtcttc gtacttcgcc atgatttttt gagtgcgcgg cgtggggctg
     8641 gcgaggaatt cagggccgcc atcgtatggc gtataattca actggatgaa accacgcacg
     8701 tctacggcgt tctgccattg gccggcattg aaacccgccc aagccttttc aaaacgttcg
     8761 cctagctgtc tggtcatttt tctatctcct gacttttttt aaagggaaaa atgttccaga
     8821 cttgagaggc tgccggcgga gatgcttttt ctaaaaaaac ttttagcgat atggcacaaa
     8881 caacaataaa aacaaaaacc gccaggaagc ttttctcaag cgcccaggcg gtcggctttt
     8941 aactgttcca ctataaatct ccgcgattcc cgatggttag ttccatcaat atccgccagt
     9001 ttcacggagg gcttttttat ctaacatgta atattgtaga cgcgatttgc aaatatgtca
     9061 agttaagtaa agaattttaa cattgttacg taactgcctg taaatgaaga taataaataa
     9121 atgcaggcga aagattcgat gaaaaaatgt tttgtcgggc ttgacagttt ccggtttgcg
     9181 tgatactctg cggccatgaa tttacggaat aacatcgtga agaaaaacac tgcagttgac
     9241 atgaatgtca ctgctgtttc cggcgcgatt ataagaatta tccagtgcct cgcagtaatt
     9301 cttgtaatta tttgcgtatg taccgttccg taagagatga ttcaatttcg ataaaatttt
     9361 taaaaagaaa aagaaaaaga aaaagctctt cggaacagtt ccgaggagct tttttttaga
     9421 tttttacatt agaacctatc ccaaaaacat gggcgtcgcc gaaatcgagc aattttacgg
     9481 gctg
//
LOCUS       DAONFI010000047         9398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8384_length_9398_cov_3.644868, whole genome shotgun sequence.
ACCESSION   DAONFI010000047 DAONFI010000000
VERSION     DAONFI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8384_length_9398_cov_3.644868"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..300)
                     /gene="infA"
                     /locus_tag="PLK08_02515"
     CDS             complement(73..300)
                     /gene="infA"
                     /locus_tag="PLK08_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPS52402.1"
                     /translation="MAKGDNIELEAVVKQALPNAMFRLEIEMGENKHEIIGMISGKMR
                     RHYIRITPGDKVLVEMSPYDLTKGRIIYRQR"
     gene            581..1756
                     /locus_tag="PLK08_02520"
     CDS             581..1756
                     /locus_tag="PLK08_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPS52403.1"
                     /translation="MVRFFPSAAGYGCKTRMFVVIIPKMEFVIGLIVGLLLGGAAVWI
                     FLSGRSRRLSAEISTAREELAAQLRETFDSLAARALDANSQRLTERTETALNAKKELI
                     DQSVKAINDRLNSMGEYFRTLEGERKTEFGRLSASVSTLAVTTGELHKVLASTQRRGA
                     WGERMAEDILRLLGMKEGVNYRKQSADDAESGRPDFTFPLPNDLVVNMDVKFPLEHYK
                     GYIDADDDQARDVQRRQLVNDVKGHIREVARRGYVDSQGSTVPYALVFIPSEQLYSLV
                     LDTQPDIIDEALASHIVLASPITLYAMLAVIRQAAENINVMKTADEVITLLGGFNQQW
                     GKYKEAVEKLGKYIEQAAGQFNILTTTRTNMLQKPLDKIEDIRQRRGLPQLEADEDE"
     gene            1872..3650
                     /locus_tag="PLK08_02525"
     CDS             1872..3650
                     /locus_tag="PLK08_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPS52404.1"
                     /translation="MQDLSQRISSEFLPLVQQPSQYVGLEVNARMKNVNAAEVTVAMC
                     FPDAYTIGISHLGSQILYHVLNDIDFVACDRAYCPPPDGEKALRETGIPLFGWESRCA
                     LCDFDVLGFSLGYELCVTNVLTMLDLAGIPLLASQRGDDVPLIIGGDAMADTPEPLAD
                     FFDVFLPGDGEDAMTKLAGFLRDVKRSRKSGPKLSRNELLLRIAREIPNAYVPCFYQP
                     DEFGCPRPMRDDVPAEIHRACLTELDTFPMHSRQLVPLCEGVQERVVVEVMRGCPNGC
                     RFCQAGQVRLPVRRRSVDEILSVIRQAVEATGYDEVSLLSLSTSDYPNLDELILKLND
                     ELAGRKVSVSLPSLRVDKQLTVLPKLTAAVRKGGLTIAAEAGTERLRRAIRKRITEED
                     MLAGVKAAWESGYQTVKIYFMAGLPGETQHDIEQIHWLCRRLSNTRRDVDGHRGAITA
                     SVSWLVPKPHTPMQWEPMQRAEYFWKVRETLIEMCRKTPVNVKFHRIEQSLLEALMCR
                     GGRDVGCAILRAWELGARMDGWNETWNFELWQQAIAETGVDFDAVVHTPFHPDMPMPW
                     SHIKCFRDDDFLREQRSRMYDELEIS"
     gene            3836..6331
                     /locus_tag="PLK08_02530"
     CDS             3836..6331
                     /locus_tag="PLK08_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:panthothenate symporter"
                     /protein_id="HPS52405.1"
                     /translation="MQLIDWIIVAIPLFIVLYVGIKTQKYVKGVSDFLTAGRVAGRYV
                     ICVAGGEAAMGLVSLAAMFENYYKSGFAYSFWSRLATPIGIVMALTGYCIYRYRETRA
                     MTLGQFLEIRYSRKFRLFAGILQSIAGIINYSLFPAVGARFIVYFCGLPANTEICGYT
                     FPTFALLMAAFLAIAVFVATLGGQITIMVTDCIQGILSYPMYAIIVGFIIYKYSWSND
                     MAPTVMNRPKGQSMINPFDITELRDFNLFYVIVGIFENILNRMGWAGTQGYNAAAKNA
                     HEQKMGSILGAWRVGFSTMMYILLALVAFMYLHSEKFAGGPTGADACRSELAVKTIND
                     VVRGDDLTGVRSELITYVKTGNMTSSIEDLISRGKSYEARKHAERERIKYGGDENDVL
                     TVRTDATAVNAGDREKIMDTVKNALKGVDPHSAQTFATIFGQMRVPMTLRYLLPVGIT
                     GIFCALCIFLLISTDTTYLHSWGSILVQDVILPIRGKPFTPRQQLFLLRILIASVAVF
                     AFFFSFFFGQVDYILMFFAITGAIWMGGSGPCIVGGLYWKRGTTAGAWVALISGSLLA
                     FSAIFLQSYWADVVYPWLYSHEMVDSMAIWLKTLSSPFEPIIKWRMNSTKFPINSKEI
                     LAMSMLISVSLYVIVSLLTCKKPFNMDRMLHRGKYAREGDGLKKKNRSLKNILLSFVG
                     IDSQYTRGDKILAWSVFGYSLVWRFLICFVVVAVWNVISPWSNHWWSVWFFIDNFVIA
                     CTIAVVSTVWFTWGGTRDLCRLFRDLAVKETNVLDDGRVIDNVSADDVEMVEDVDKVN
                     IEEAHIEERILDEELKEKGDIEDLRNLEKHSKE"
     gene            6481..7491
                     /gene="pdxA"
                     /locus_tag="PLK08_02535"
     CDS             6481..7491
                     /gene="pdxA"
                     /locus_tag="PLK08_02535"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPS52406.1"
                     /translation="MSDERPVIGLTMGDPCGIGAEIIVRALSQPAIKSLARYVVFGLS
                     DQMEYTAGQLELPWGAYRDRHEDIRRYASDLVVLDYDEYVVPPTMPRGSSRIGGQTSM
                     AFCTDAIAAAQAGLIDAIVTAPISKSSWKMAGYKRFPGHTELLADKCKVRDVAMMFVA
                     PRLRVTLATIHDPIHALRDKLTIGRVYNPIELSHKALRNWFGIEKPRIGVCGLNPHAG
                     ENGHIGDDESRVIEPAILLAAENGIDVHGPYPADTIFNKALAGEFDLVIAMYHDQGLI
                     PIKILGWEDAVNVTLGLPIIRTSPDHGTAFDISGKFKANPSSMVAAIKLAAELVVKNR
                     IR"
     gene            7681..8931
                     /locus_tag="PLK08_02540"
     CDS             7681..8931
                     /locus_tag="PLK08_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52407.1"
                     /translation="MSENNKPQITVVGGGMITQIQLLPTIYHMQREGLVGDIHVCALN
                     ARPLRTIIDSPALNAAFPGQSIIAHPDPRMEKDLDKVYPDSFREVLAAAPRGSICVVA
                     LPDQLHYMAVKAALENDLHVCTVKPLVLKFAQAKEIEQLAYERGLVVGVEYHKRFDYR
                     NLMARKAYRSGKLGEFRLAQAQMIEPYYYRESNFQNWCTCENSDMFTYVGCHYVDLVA
                     FITGLKPKRVSIYGIVDKYPNGRDGYLWTDGRVIWENGASLSVVDAIGYPNEAAGGNA
                     QGLTMWCQGDRDAALLVHSDQYRGVKCHYNFKGTDPGDTLYAEPNPDYFQLLGMGGDG
                     LVPAGYGHRSAEFIIRQCIAASRLAGKADRQKFIKRLDADGIMATPANSSYNELVIEA
                     GRMSITHDGREVEIDYKTNTVKFI"
     gene            8955..>9398
                     /locus_tag="PLK08_02545"
     CDS             8955..>9398
                     /locus_tag="PLK08_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS52408.1"
                     /translation="MATKEFPLTPQKVPAVNTKYRKICTQIPAPQSVEILETLRRCEP
                     RSMGGQPPIIWHSGKDCTISDPYGNTWLDFSAGVLVTASGHGRPEIVKAIAGMAEQGL
                     YHAYCFPTIIRAKLAEKICKFMPKPLARVFLVTTGSEATECCIKLA"
BASE COUNT         2258 a         2307 c         2548 g         2285 t
ORIGIN      
        1 ccagcccgca aaattgctcg atttcggcga cgcccatgtt tttgggatag gttccaggaa
       61 aatatcaaaa aatcagcgct ggcggtagat tattctgccc ttggtcaggt cgtagggact
      121 catttccacc agtaccttgt cgccgggtgt gattctgata tagtgacggc gcatcttgcc
      181 ggatatcatg ccgatgattt cgtgcttatt ttcgcccatt tcgatttcaa gcctgaacat
      241 cgcgttgggc aacgcctgtt tgactacggc ttccaattct atgttgtcgc cttttgccat
      301 atatttcctt gcaactatct cccgataaat ccaactgatg ccgccgactc gcagttattg
      361 ttattataaa aaatattcaa gcgtctcaaa aatccggaaa gcttaacatg ctctaacgga
      421 actactgcgg aataaacccg caaaaccgcc aataacatat tcgactcgct tttacataat
      481 atacggggcg taaatcctaa cattgccaac accccgcgtc aagtcacttt ccgccaaaga
      541 tgactaaaaa tacttgcaaa tcaacaggat atgccggtgc atggtcagat tctttccgtc
      601 ggcggctgga tatggctgta aaacgcggat gtttgtagtt ataatcccga agatggaatt
      661 tgtaatagga ctaattgtag gattgctgct tggcggagcg gcagtgtgga tatttttatc
      721 gggcaggtca aggcgtttat ccgccgagat atcgactgcg cgcgaggagc tcgccgcgca
      781 gcttcgcgag acgttcgatt cgctggcagc ccgcgcgttg gacgcaaaca gccagcggct
      841 gaccgaacgc actgaaaccg ccctgaacgc gaaaaaagaa cttatcgacc agtccgtcaa
      901 agccatcaat gaccggctga acagcatggg cgaatatttc cgcacgcttg aaggcgaacg
      961 aaagacggaa ttcggacggc tgagcgcgtc ggtttccacg ctggccgtca ccaccggcga
     1021 acttcacaaa gttctcgcca gcacgcaacg tcgaggcgcg tggggagaac gcatggcgga
     1081 agacattctt cgtctgctcg gaatgaagga aggcgtcaac tatcgcaaac agtcggctga
     1141 cgacgctgaa tcgggcaggc cggatttcac atttcctctg cccaacgacc tggtggtgaa
     1201 tatggacgtc aaattcccgc tggaacatta taagggatat atcgacgccg atgacgacca
     1261 ggcaagagat gtccagcgcc gtcagcttgt caacgacgtc aagggccata ttcgtgaagt
     1321 cgcccgccgc ggttacgtgg attcgcaagg ctcgaccgtg ccgtacgcgc ttgtgttcat
     1381 accatccgag cagttataca gccttgtgct cgatacccag ccggacatca tcgacgaggc
     1441 tctcgccagc catatcgtac ttgccagccc gattacgctc tatgcaatgc ttgccgtcat
     1501 ccgccaggct gccgagaaca tcaatgtcat gaaaaccgcc gacgaagtca taacgttgct
     1561 gggcggattc aaccagcagt ggggaaaata caaagaagcc gtcgaaaagc tcggcaaata
     1621 tatcgaacag gctgccggcc aattcaatat tcttacgacc actcgcacca atatgctgca
     1681 aaaaccgctg gataagatag aagacattcg tcaacgacgc ggtctgccgc agttggaagc
     1741 cgacgaagac gaatgagatt ttgcttaaaa caccacatgg tttcaataat gttatcgccg
     1801 gtttgcctga gttcggttct atctgttgtt acagaatctg aaaaaaaatc acgaaggata
     1861 ttttttaaaa aatgcaggat ttatctcaac gtataagcag cgaatttctg cccctcgttc
     1921 agcagccgtc gcagtacgtc ggccttgaag tcaacgccag aatgaaaaac gtcaacgccg
     1981 ccgaagtgac cgtggcgatg tgttttcccg acgcatacac catcggcata agtcacctgg
     2041 gcagccagat actttatcac gttctgaacg atatcgattt cgtcgcatgc gaccgcgcgt
     2101 attgtccgcc gccggacggg gagaaagctc tgcgcgagac cggcattccg ctgttcggtt
     2161 gggaatcccg ctgcgcgctg tgtgatttcg acgtacttgg tttttcgctc ggctacgagt
     2221 tgtgtgtgac taacgtgttg acgatgcttg acctggcggg tattccgctg cttgcgtcgc
     2281 agcgaggcga tgacgtgccg ctgataatcg gcggcgatgc gatggctgac acgcctgagc
     2341 cgctggctga ttttttcgac gtctttctgc ctggtgacgg cgaggatgct atgaccaaac
     2401 tcgccggttt tttgagggat gtcaaacgca gccgtaaatc tggccccaaa ctgtcgcgga
     2461 atgaacttct gttgcgcatc gcacgggaaa tcccaaatgc ttatgtgccg tgtttttatc
     2521 agccggacga attcggctgt ccacggccga tgcgcgacga tgtgcctgct gaaattcatc
     2581 gagcctgcct gacggaactt gacacgtttc cgatgcattc caggcagctt gtgccgttgt
     2641 gcgaaggcgt gcaggagagg gtcgtcgttg aagttatgcg aggctgtccc aatggctgtc
     2701 gcttctgtca agccggacag gtccgtctgc cggttcgccg tcgcagtgta gatgaaattc
     2761 tttccgtcat caggcaggcg gtggaggcta cgggctatga cgaggtttca ctgctcagcc
     2821 tttccacgag cgattatccg aacctcgatg aacttatttt gaagctcaat gacgaacttg
     2881 ccggaagaaa ggtctcggtt tcactgccgt cgctgcgcgt cgataaacag ttgacggttc
     2941 tgcccaaact taccgccgcc gtgcgcaagg gcgggttgac catcgccgcc gaggcaggta
     3001 ctgaacggct acgccgcgct attcgaaaac gcattacgga agaggacatg cttgccggcg
     3061 tcaaagccgc ctgggaatcc ggctatcaga ccgttaaaat atatttcatg gctggcctgc
     3121 ctggcgagac gcagcatgac atcgagcaga ttcattggct gtgcaggcgg ctgagcaata
     3181 ccagacgcga cgtcgacggc catcgaggcg cgataaccgc cagcgtttca tggctggtgc
     3241 ccaagccgca cacccccatg caatgggagc ccatgcagcg ggcggaatac ttttggaaag
     3301 tgcgggaaac gcttattgag atgtgccgca aaacgccggt aaatgtgaaa ttccatcgta
     3361 tcgagcagtc gctgctggag gcgttgatgt gtcgcggcgg cagggatgtc ggttgcgcga
     3421 tactgcgagc gtgggaactt ggcgcaagaa tggacggctg gaacgagacg tggaatttcg
     3481 aactatggca gcaggctatc gccgagacgg gtgtggattt cgacgccgtg gttcatacgc
     3541 cgtttcatcc ggatatgccg atgccgtgga gtcacataaa atgttttcgc gacgacgatt
     3601 ttttgcgcga acagcgaagc agaatgtacg acgaacttga aatttcttga cactaaccga
     3661 tggtgttgtt agattcacaa atgggattat atggttttaa agccgtagaa tcccgacttg
     3721 atatatttaa aatatgttct gaaacagttg ctgattccgg tatgttccaa tgtgttttgc
     3781 ccgaagtatt gggctgttta ttgtctacaa attttgtaat gatgctgagg taaacatgca
     3841 acttattgac tggattatcg tagcaattcc gctttttatc gttttatatg tcggcattaa
     3901 aacacagaag tacgtcaagg gggtttctga ttttctcacg gcaggcagag tagccggcag
     3961 atatgtcatc tgcgttgccg gcggcgaagc ggccatgggg cttgtcagcc ttgccgccat
     4021 gttcgagaat tattataagt caggtttcgc atacagtttc tggagccgac ttgccacgcc
     4081 gataggtata gtcatggcgt tgaccggata ctgcatatac cgctatcgtg aaacgcgagc
     4141 catgacattg gggcagtttc tggaaattcg ttacagccgg aaattccgtc tctttgccgg
     4201 cattcttcaa tcaattgccg gcataataaa ctactccctg ttccctgcgg ttggtgcgag
     4261 gtttattgtc tatttctgcg gattgccggc gaataccgaa atatgcggtt atacgtttcc
     4321 gacattcgcg ttgttgatgg cggcgtttct tgccatcgct gtatttgtcg ccactcttgg
     4381 cggccagata acgattatgg tgacggactg tatccagggc attttaagct accccatgta
     4441 tgccattatc gtcggcttta tcatttacaa atattcctgg tcgaatgata tggcgcctac
     4501 ggtgatgaac cggcccaaag gccagagcat gataaatccg tttgacatta cagagcttag
     4561 ggattttaac ctgttttatg tgattgtggg gatatttgaa aatatcctga atcgcatggg
     4621 ctgggccgga acgcagggat ataacgctgc cgccaaaaat gcccatgaac agaagatggg
     4681 ttcgatactg ggtgcatggc gggtcggttt ttcaaccatg atgtatattc ttcttgctct
     4741 tgtggcgttc atgtatctcc acagcgaaaa gtttgccggc ggcccgactg gcgccgatgc
     4801 ctgtcgttcc gagcttgccg tcaaaaccat aaacgacgta gtcaggggcg acgacctgac
     4861 aggtgtgcgc agtgagctta taacctacgt caaaaccggc aacatgactt cttctataga
     4921 agacctgata tccaggggca aatcttacga agccagaaaa cacgccgaac gcgaacgaat
     4981 caaatatggc ggtgacgaaa atgacgtact gactgtccgt accgatgcga ctgccgtaaa
     5041 tgccggcgac cgtgaaaaaa tcatggatac cgtcaaaaat gcactcaagg gtgttgaccc
     5101 tcattccgca cagacgtttg ccacaatttt cggccagatg cgtgtgccga tgacgttgcg
     5161 ttatctgctg ccggtcggca ttaccggcat attctgcgcg ttatgtattt tcctgctgat
     5221 atcgacggat accacgtatc ttcattcgtg gggcagcatt ctggtgcagg acgtgattct
     5281 gccgattcgc ggcaaaccgt ttacgccgcg gcagcagctt ttcctgttgc gtattttgat
     5341 agcatccgtc gcggtttttg cattcttttt cagtttcttc ttcggccagg tggattacat
     5401 tctgatgttt ttcgccatca ccggagcaat ctggatgggc ggttctgggc cgtgcattgt
     5461 cggcggatta tattggaaac gcggcacgac ggctggggca tgggttgcgt tgataagcgg
     5521 ttcgctgctg gcgttttcgg cgatattcct gcaatcgtat tgggcggacg tggtttatcc
     5581 gtggctgtat agccatgaga tggtggattc aatggctatc tggctcaaga cgctttcatc
     5641 gccgtttgaa cccataatca agtggcgcat gaacagtacc aagttcccga ttaattcgaa
     5701 ggaaattctt gccatgtcga tgctgatttc agtatcgctg tatgtcatag tttcgctgtt
     5761 gacctgcaaa aaaccgttca atatggatcg catgctgcat cgcggcaaat atgcgcgtga
     5821 aggtgacggc ctcaagaaaa aaaatcgcag cctgaaaaat atattgctga gtttcgtcgg
     5881 catagacagc cagtatacca ggggcgataa aattcttgca tggtcggtgt tcggctattc
     5941 gctcgtgtgg cgttttctga tatgtttcgt cgtagtcgcc gtctggaatg tgatttcacc
     6001 gtggagtaat cattggtggt cggtgtggtt tttcatcgat aacttcgtaa tagcatgtac
     6061 tattgccgtt gtttctacgg tatggttcac ctggggcggc acgagagatc tttgccggtt
     6121 attcagagac ctggctgtca aggaaaccaa tgtgctcgac gatggccgtg ttatcgacaa
     6181 tgtaagtgcc gacgacgttg aaatggttga agatgtcgat aaagtaaata tcgaagaggc
     6241 acacatcgag gaacgtatac tcgacgaaga actcaaggag aagggcgata tcgaagattt
     6301 gcggaatctc gaaaagcatt cgaaagaata acccgtattg tcacagttga ttatgaacgg
     6361 gacaggtcgc tttttaaggc ctgtcccgtt ttttatctgc ttgatatttt tgccgccgcg
     6421 gtgcaaaata tataatctgc aaatctagta tattttggcg tcgattaatg ggaatgataa
     6481 atgtcagatg agcggccggt aataggattg accatgggcg acccgtgcgg catcggggcg
     6541 gagataatag tgcgcgcact ttctcagccg gcgataaagt cgctagccag atatgtggtt
     6601 ttcgggctga gtgaccagat ggaatatacc gccggccaac ttgaattgcc gtggggggcg
     6661 tatcgcgacc gccatgaaga catccgcaga tatgccagcg acctcgttgt gctggattac
     6721 gacgaatacg tcgttccgcc gacgatgccg cgcggttcga gcagaatcgg cggccagact
     6781 tcgatggctt tctgtaccga tgccatcgcc gccgctcagg ctggacttat cgacgccata
     6841 gtcaccgcgc cgatttcaaa atcgagttgg aagatggccg gttataaacg ctttccgggc
     6901 cataccgaac tgcttgccga caaatgcaag gttcgggatg tcgcaatgat gttcgtggca
     6961 ccgcggctgc gcgtgacgct ggcgacgatt cacgacccca ttcacgccct gcgcgacaaa
     7021 cttaccatcg gcagggttta taacccgata gagctgtcgc acaaggcgtt gcggaactgg
     7081 ttcggcattg aaaagccgcg catcggtgta tgtggactta atccgcatgc cggcgaaaac
     7141 ggccatatcg gcgacgatga atcccgcgtc attgagcctg cgatactctt ggccgccgag
     7201 aacggcattg acgtgcatgg cccgtatccg gctgacacga tttttaacaa ggctctcgcc
     7261 ggcgagttcg accttgtcat cgccatgtat catgaccagg ggctgatacc cataaaaatt
     7321 ctcggctggg aggatgccgt gaacgtcacg cttggactgc cgataatccg taccagtcct
     7381 gaccacggaa cggcatttga tatttcaggg aaattcaagg ctaatccgtc gagcatggtg
     7441 gctgccatca aacttgcggc ggaactcgtc gtcaaaaaca gaatcaggtg attttgaggc
     7501 gatatacgct ggaaagcgga ttgtaacggg tgcgggaacg tggtttggcg gctgaaaagg
     7561 tctggacttt tattcaatga aacgggtata tgattccagc attaacatat cttttcgtcg
     7621 gttatgagtg ttaaagtata tgtacaggaa ttcaataaca cagatgaaag gaatcaataa
     7681 atgtcggaga ataacaaacc tcagataacc gttgtcggcg gcggcatgat aacgcagata
     7741 cagcttttgc cgacgattta tcatatgcag cgggaagggc ttgtgggcga cattcacgtt
     7801 tgtgcgctca acgcccggcc gctcaggacg ataatcgatt caccagcgtt gaatgcggcg
     7861 tttcccggcc agtcgataat tgcccatccc gacccgcgga tggaaaaaga cctcgacaag
     7921 gtttatcccg attcgtttcg tgaagtgctg gcggctgccc cgcggggcag catatgcgtg
     7981 gtggcgctgc cggatcagtt gcactacatg gccgtaaaag ccgccctgga aaacgatttg
     8041 cacgtctgta ccgtgaaacc gctcgtgttg aaattcgcgc aggctaaaga aatcgagcaa
     8101 ctagcatatg aacgcggttt ggtcgtcggc gtggaatatc acaagcggtt tgactaccgc
     8161 aatctgatgg cgagaaaagc atatcgcagc ggcaaacttg gcgaattccg gctggcgcag
     8221 gctcagatga ttgagccgta ctattatcgc gagtcgaatt tccagaactg gtgcacgtgc
     8281 gaaaattccg acatgttcac ttacgtcggc tgtcattacg tggacttggt ggcgttcata
     8341 accggtttga agccaaaacg cgtcagcata tacggcatcg tggacaaata tccgaacgga
     8401 cgcgacggat atctctggac ggacggcagg gtgatatggg aaaacggcgc aagcctgagt
     8461 gttgttgacg caatagggta tcccaatgaa gcagccggcg ggaacgccca aggccttacg
     8521 atgtggtgtc agggcgacag ggatgccgcg ctgctcgtac attccgatca gtatcgcggc
     8581 gtaaaatgtc attacaattt caagggcact gaccccggcg acactttata tgccgagccg
     8641 aatcctgatt attttcagtt gctcggcatg ggaggcgatg gacttgttcc tgcgggttac
     8701 ggccatcgtt cggcggaatt catcattcgc caatgcatcg cagccagccg acttgcaggg
     8761 aaggctgacc gccagaaatt catcaaacgg ctcgatgccg acggaatcat ggctacgccg
     8821 gctaacagct catataatga actcgtcatc gaagccggcc gtatgagcat cacgcacgac
     8881 ggacgggaag ttgagataga ttacaaaaca aatacggtaa aattcatcta aaatcggaaa
     8941 gaaggttgat taaaatggct accaaagaat ttcctcttac gcctcaaaaa gtgccggctg
     9001 tcaataccaa gtatcgcaaa atctgcaccc agatacctgc accgcagtcg gtagagattc
     9061 tcgaaacgct ccgcagatgt gagccgcgca gcatgggcgg ccagccgccg attatttggc
     9121 acagcggcaa agattgcacg ataagtgatc cgtacggcaa tacgtggctg gatttttccg
     9181 ccggcgtact cgtgaccgcc agcggccacg gcaggcctga aatcgtcaag gcaattgccg
     9241 gcatggctga gcaggggtta tatcacgcat actgtttccc gacaattatc cgcgcgaaac
     9301 tcgcggaaaa aatctgcaag ttcatgccaa aaccgctggc tagagttttt ctggtaacga
     9361 ccggttcgga agctaccgag tgttgcatca aactcgcc
//
LOCUS       DAONFI010000048         9397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8386_length_9397_cov_3.938878, whole genome shotgun sequence.
ACCESSION   DAONFI010000048 DAONFI010000000
VERSION     DAONFI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8386_length_9397_cov_3.938878"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLK08_02550"
     CDS             complement(<1..459)
                     /locus_tag="PLK08_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52409.1"
                     /translation="MHIIFGLILIAFFIAALCVFGAHGWQMVRRERMLAGQANDAGLK
                     FSREDPFEIPLKYAAFSLMRCGHGQIAYNVAYGRLDSFDVQALDFSYEIGDGFERSTH
                     RYGVIIFAVPVCGHMLMWPQCKTVHNMPLLTADNIVEVVGPWQYCGSGSLA"
     gene            complement(521..1810)
                     /locus_tag="PLK08_02555"
     CDS             complement(521..1810)
                     /locus_tag="PLK08_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52410.1"
                     /translation="MHNKKAAMLILGIVILGGSLVYKSSQPIFAQADSTAVNGKTKVV
                     KLVDGRTLTGVVREIKDGIELETKFGVVRFKRDQIESMTDVAPKADEYAARKAKIDES
                     SSKDFYELARWVWDNYRNDTDMLQKAREDIEESRNIGPANERAKHLLRQVDAVLNGSS
                     QPITTGHLIKPTGKNTAGNGTVADVDKYLISQEDIYWIRLMELAAKDSVSVTFMNNAV
                     NRYIELMKGKTVDGWDRIGNDRRFRAASPTEKVQEILRNQFDNTDLLKDVQITRDPSF
                     MIEFRSKVWPQVKQYCASPQCHGGPQPKGGFKFFVMPNNVKADYTNFVILSCFRNSEG
                     RKLIERDDVDTSLLLQYGLNPKVARFVHPKVNNQDITFMYPSLNAKTYKTASRWIQSL
                     TRPEPNYHLQYKVPFGMKIDVSGRPYIPSEDAGSSGK"
     gene            complement(1877..1951)
                     /locus_tag="PLK08_02560"
     tRNA            complement(1877..1951)
                     /locus_tag="PLK08_02560"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1915..1917),aa:Cys,seq:gca)
     gene            complement(2169..5576)
                     /locus_tag="PLK08_02565"
     CDS             complement(2169..5576)
                     /locus_tag="PLK08_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52411.1"
                     /translation="MTTGRHLVMLMLAAIFVASSVSLAQERKSAYERLDDEALSDVLT
                     KLRMSELLEALAERTDNINLRISAALTKADNAQDPALREKYLAQAAELMRQNIKDIED
                     RLDGIQNEDEKEEALINYYNLRMKQIDVAVVFRGKPYIDRLLLLLGGDADREELANLT
                     AIGASQLRSLQSSTKPADELERLKEYLTENEKYLKEEKDPAKQKDLISEIASQKQRIA
                     DAEDCLKWGNRSLDADIKKAMTNPRYQVYLIPELRDIKNRLTYKAAFLDFYEALAMPV
                     TIQQKDPKSGKIQFVPNNVRIDRLNKALSEIKPFIAEVDSPAQPFARLLQARCLRELG
                     VTMNSGDSSQEMRKKGMEYYVEAGKILDTLAGPEGKKELLVETLFEIARNTIEQAASL
                     ISQNQVAEGEKKFAMAQTDINNFVKEAAAVSSQIGIDVKKLVLDYYLYEQWATSLRSI
                     GQEGKALAYDKKIQESFMQFLKQYRNPAIQAQVGEMFAKKFRGIDPDKLDPVIVLLLA
                     KMESTQADREQKGHTFAELSPELQKQVSEHRKKAIEMLDKIRISQSDSAKSALPQVLF
                     DLGILYVQQGHEPGNNLKAAHTFRELVEKFPGDEKAKTAALNAVKINYEIIDARQAEN
                     KSIDANLRNEYVASLRTLLKNWGNDEDAAPFYFDLAWQCDKLAENTGEEKWLVEAIGN
                     YEKVPASSPQYAAARGYALDNRMRMLESKDAATQKQMAGQLIADLQAYANQMQSLYQK
                     TEDETRKAEYRDLGAMAEFNANRLAATAMGQIDKAIDGIAKLEARWPDSSVQQTAQEF
                     AVRTQVNSGRIDGAIKGMDSFKQKYGSMAASGLMQLVVQKIRESIQSLVASGNNPDKL
                     SDYRKAYLKFARQVYDQQYAKAAVDEKYQLLSMYADALAQSDEAANAQQALGLYDEMK
                     QIEQSRQAAVQENVRNAVAQRLKSLDVAGDDKKKLQAMQKAVTDAAREFQLQDWIVTN
                     SNFIAAKDALKFLDAPKTPDEKKLSEKDWKEEYRRRIVRAQRFIRTSLEAIEKQAMFQ
                     STTLDAGMLAGRAHCYMVLKDYNKAIELYRKLLSGLTASMDHGMYWNSQIELCQSLLD
                     NAKDARDYKAIALYIGQLQGEDPDLGGVRFRGRLNRILSQAQEKAGI"
     gene            complement(5638..6141)
                     /locus_tag="PLK08_02570"
     CDS             complement(5638..6141)
                     /locus_tag="PLK08_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPS52412.1"
                     /translation="MPINEIMQIEEGSEEQHYVAPRQKRQMDKKSMQPPLTPMIDVTF
                     ELLLYFLLTSTFRKDEGQIPASLPAAGGAASEAPPFRPIRIIIMPQGSLRDQVVYTVD
                     NNPPTDKPEMLYAYLESRAKTGDPAEIPVLIKSDGFARWKFVVEAFNAAVRAQFKKIA
                     FSGDGAG"
     gene            complement(6192..6734)
                     /locus_tag="PLK08_02575"
     CDS             complement(6192..6734)
                     /locus_tag="PLK08_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPS52413.1"
                     /translation="MAKKQITGSIQEVNLTPMIDCTFQLIIFFILTAQMASQELAKVS
                     LPEPVKSMAIGADPDKPDQPTNLNNVTVNVVNKYGDSDDNRQMELAGKAECYQLGQNK
                     IDAGNIEALTAAIKERKAQILSDKNIKNVKEGDIIVEIRADKDIVFQDIEPVMRAAAE
                     AKINKMSMTALVDPEHRVKR"
     gene            complement(6812..7435)
                     /locus_tag="PLK08_02580"
     CDS             complement(6812..7435)
                     /locus_tag="PLK08_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPS52414.1"
                     /translation="MNYFDILVVGGGTIGYILWAMSVAVVALAIHYFMSIRRVNIAPD
                     AVRDQLNELFAAKQYREAIDITAEQSDFLSYIVHSSLKEAPYGFPAMERAMEEAAEER
                     TTKLLRNLEWLSLMGNIGPMLGLLGTVWGMILAFFQIVEAGAPNPKDLAGAIGIALVT
                     TLLGLTVSIPALSLYSMLRTRIDALTSESVKVAQEMISAFRPEAKRQ"
     gene            complement(7531..8721)
                     /locus_tag="PLK08_02585"
     CDS             complement(7531..8721)
                     /locus_tag="PLK08_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52415.1"
                     /translation="MRKIAAICPILLLLLSAVATADMINLNDGKAFEGKIKDFSAQSF
                     EITVRLRANTLEQPVAEIASMTINGDDAFNKAEKALAAGDLGTAVKSYGAALKAANSD
                     WMKQLIEIRRYQAVARSKRIDIAVKAWIKLVETFESCSAILALSPANPGNESVNKSAV
                     DVINSQIKRLAADRKTNRKFIQALLKTKMKIQQAMGDNDGAARTAVILSNIDSADLTG
                     EHPVTPADSSAADSDENSSKTSDARRPSSSTPAAPLAAGKTADLLVLGQLYNAGKYDV
                     VIDSIKENLNKYDDEKLPAALTLLGRAQMEKAKRMNNDKGLLIDSGLNLMRVYAEYND
                     SESGPEALFYGAKVNELLGQTAGARAALTELAEHYAEEGQAINPWVKKAQQELQALNQ
                     AKDD"
     gene            complement(8721..>9397)
                     /locus_tag="PLK08_02590"
     CDS             complement(8721..>9397)
                     /locus_tag="PLK08_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPS52416.1"
                     /translation="ITKVSTDTGATKNAISVIGVEGASAGSAGADMGMATSSGGGPGN
                     RFFGLGGGNAYNVVYVIDGSGSMEISGAFKAVCDEMYRSIARLSDKQTFAVILYTAGK
                     PEECSRSLMLANKKNKKKAADFLSGYSGSGLSNPIPALDRAFQVLRGAVKPGRLVYLL
                     SDGEFKGEGVSNDKVLAAIRALNSNKKVNINTLLFGSKEKAAIQVMQQIAEQNKGQYK
                     YIEWGD"
BASE COUNT         2126 a         2530 c         2178 g         2563 t
ORIGIN      
        1 ggccagactg cctgagccgc aatattgcca tgggccgacg acttcaacaa tattgtcggc
       61 agtaagaagc ggcatattat gaacggtttt acattgcggc cacatcagca tatgtccaca
      121 gaccggcacg gcgaagatta tcacgccgta tctatgcgtc gacctttcaa agccgtcacc
      181 tatttcataa ctgaaatcca gagcctgtac gtcaaacgag tccagccggc catacgccac
      241 gttataggca atctgaccat ggccgcaacg catcagagaa aacgcggcat atttaagagg
      301 tatttcaaaa gggtcttcac gggaaaattt caaaccggca tcattcgcct ggccggcaag
      361 catgcgctcg cgacgaacca tctgccagcc atgtgctcca aaaacgcaaa gcgctgcaat
      421 gaaaaatgct atcaggatta acccgaaaat aatatgcatg gctgtatgct aatccgccgc
      481 gacgtttccg actgtgcgtc gctcttacta tatcatttca ttatttgccg gaactgccgg
      541 catcttccga cggaatatac ggcctgcctg atacgtctat tttcattccg aacggaactt
      601 tgtattgcag gtgatagttc ggctcagggc gggtaaggct ctgtatccag cggctggcag
      661 ttttataggt tttggcatta aggctcggat acatgaacgt tatatcctga ttgttcactt
      721 ttggatgaac gaatcttgca actttcggat tcaagccgta ctgcaaaagc aatgaggtgt
      781 caacatcgtc gcgttcaatc agtttgcggc cttcactgtt gcggaaacag ctaagaataa
      841 cgaaatttgt atagtcggct tttacgttat tgggcattac gaagaactta aagccgcctt
      901 ttggttgagg cccgccgtga cattgcggcg aggcacaata ctgcttaacc tgcggccaaa
      961 ctttcgaacg gaattctatc atgaaagacg ggtcacgcgt aatctgaacg tctttcagca
     1021 ggtcagtatt gtcaaactgg ttgcgcaata tctcctgcac tttttccgtc ggcgatgccg
     1081 ccctgaagcg tctgtcatta cctattctat cccagccgtc taccgtttta cctttcatca
     1141 attcaatata cctgttaacg gcgttattca taaaagttac gctcacgctg tccttagccg
     1201 ccagctccat gagacgtatc cagtaaatgt cttcctggct tataaggtat ttgtccacat
     1261 ccgctaccgt gccgttgccg gcggtatttt tgccggtggg ttttatgaga tggcctgtag
     1321 taatcggctg agacgaccca ttcaatacgg catccacctg cctgagcaga tgttttgcac
     1381 gttcgttggc agggccgata ttccgcgatt cctcgatatc ctcccttgcc ttctgcagca
     1441 tatccgtatc attgcgataa ttgtcccaaa cccatcgggc aagctcgtaa aaatccttgc
     1501 tgctgctttc atcgatttta gctttgcgtg ccgcatactc atcagccttc ggagccacat
     1561 ccgtcatgga ttcaatctgg tcgcgtttga acctgacgac gccgaatttg gtctcaagct
     1621 caatgccgtc cttgatttca cgcacgacgc cggtcagggt tctgccgtct acgagtttga
     1681 cgaccttcgt tttgccgttg acagcggtac tgtccgcctg cgcaaaaata ggctgcgatg
     1741 atttatatac cagagagccg cccaaaatca ctatccccaa aataagcatg gctgcctttt
     1801 tattatgcat gatagacctc actctcttgc ataacacacc cacgccgcct tcagggccat
     1861 ccatacccca tgaaaaaggt ggcggtggga ttcgaaccca cgaaataacg gatttgcaat
     1921 ccgtcgcctt aaaccacttg gctacgccac cgcttcaggc ttttctttct caaatgccgt
     1981 ccgccggttc ggatagtata atgaaccgac tgcctaaatc aatgcccgta acctgtttac
     2041 atcaattttt tttgcactcg ctggcacgga tacgtttgcc accgtcagcc tgccgtttgc
     2101 aaaaatcttt aacttacacg aataacagta tttaaaagca aattaaggac attttccaga
     2161 gaaaaatctc agatgccggc tttttcctga gcctgggaca atatacgatt caatctgccg
     2221 cggaatcgaa cgccgcccag gtccggatct tcgccctgca actggcctat gtacaaagct
     2281 atagccttgt aatcgcgggc atccttggcg ttatccaaca acgactgaca gagttctatc
     2341 tgcgaattcc agtacatgcc gtgatccatg ctcgccgtca agccggaaag caatttccgg
     2401 taaagctcga tagccttatt gtaatccttc agaaccatgt agcagtgcgc ccggccggca
     2461 agcatgcctg cgtcaagcgt agtactctgg aacatagcct gtttttctat cgcctccagc
     2521 gaagtgcgta taaaccgctg tgcgcgaaca atccggcggc gatattcttc cttccagtct
     2581 ttttcggaaa gttttttctc gtccggcgtt ttaggtgcgt caagaaattt cagggcatct
     2641 ttggcggcga taaaattgga attggtgact atccagtcct gaagctgaaa ttccctggcg
     2701 gcatccgtga cggctttctg catcgcctgg agtttctttt tatcgtcgcc ggcaacatca
     2761 agcgatttca accgctgtgc tacggcgttg cgtacgtttt cctgcaccgc agcctgacgc
     2821 gactgttcta tctgcttcat ctcgtcataa aggcccagtg cctgttgcgc attggcggcc
     2881 tcatcgcttt gtgccagtgc gtcggcatac atggaaagca actgatattt ctcatcgaca
     2941 gccgctttgg catactgctg gtcatatacc tgtctggcga acttgagata ggctttgcga
     3001 taatcggata atttatccgg attgttcccg ctggcaacaa gactctgaat gctttcgcgt
     3061 attttctgca ctacaagctg cattagtccc gatgccgcca tagaaccgta tttctgttta
     3121 aaactatcca tgccttttat cgcgccgtcg attctgccgg aattgacctg tgtgcgaacg
     3181 gcgaattcct gtgcagtctg ctgcaccgaa ctgtcaggcc atctagcctc aagtttcgca
     3241 atgccgtcga tagccttgtc aatctgtccc atcgccgtgg ctgcaagcct gttggcgtta
     3301 aactccgcca tagcaccaag atcgcgatac tcagccttgc gggtttcgtc ctccgtcttc
     3361 tgataaagcg actgcatctg attggcatat gcctgcaaat ccgcaatcaa ctggccagcc
     3421 atctgtttct gcgtggcggc atccttgctt tcgagcattc tcatacggtt gtcaagcgca
     3481 tatccgcgtg cggctgcata ttgcggcgaa gaggctggaa ctttttcgta attcccgatt
     3541 gcctcgacaa gccatttttc ttcgccggta ttttctgcaa gtttgtcgca ctgccacgca
     3601 aggtcaaaat aaaacggcgc cgcatcttca tcgttacccc aatttttcag caaagtacgc
     3661 aacgacgcaa catattcatt tctgagattg gcgtcgatgg atttgttctc agcctgtcgg
     3721 gcatcgatga tttcataatt aattttgacg gcattaagcg cggctgtctt cgctttttcg
     3781 tcgccaggga atttttcaac gagttcacgg aacgtatgtg cggctttgag gttgttgccg
     3841 ggttcatggc cctgctgcac gtaaagaatg ccgaggtcga aaagcacctg cggcagagcc
     3901 gacttcgcag agtcactctg gctgatgcgt attttgtcca acatctcgat agccttttta
     3961 cgatgctcgg aaacctgctt ctgcaactca ggcgaaagct cggcaaaagt gtggcctttc
     4021 tgctcacggt cagcctgcgt ggattccatc ttagcaagca gcagaactat cacagggtca
     4081 agtttgtccg gatctatgcc gcggaatttt ttggcgaaca tctcgccgac ctgtgcctga
     4141 atggccggat tcctgtattg cttcaggaac tgcatgaaag attcctgaat tttcttgtcg
     4201 tacgccagag ccttaccttc ctggcctatg ctgcgaagcg aagtggccca ctgctcatag
     4261 aggtaataat ccagcaccag ttttttgacg tctatgccta tctgagatga aacggcggca
     4321 gcctctttaa cgaagttatt tatatcggtc tgcgccattg cgaatttctt ttcgccttcc
     4381 gccacctgat tctgacttat cagagaagca gcctgctcta tcgtgtttct ggcaatttca
     4441 aacagcgttt cgaccaaaag ttccttcttg ccttccggcc ctgccagcgt gtcgagtatc
     4501 ttgccggctt cgacgtaata ctccatgccc ttctttcgca tttcctgaga cgaatcgccg
     4561 gaattcatcg taacgccaag ttcccgcagg cagcgagcct ggagcagtct ggcaaaaggc
     4621 tgcgcaggcg aatcaacttc tgcaataaag ggcttgattt cagacaacgc tttattcagg
     4681 cggtctattc tgacattgtt cggtacgaac tggattttac cggatttcgg gtctttctgc
     4741 tgaatcgtaa ccggcatagc cagcgcttcg taaaaatcca gaaaggctgc tttatacgtc
     4801 agacgattct tgatatcgcg aagttccgga ataagataaa cctgatagcg gggattggtc
     4861 atagcctttt ttatgtcggc atccaacgaa cggttacccc atttcaaaca atcttcggca
     4921 tcggcgattc tctgtttttg cgatgctatt tcacttatta aatctttttg tttggctgga
     4981 tctttttcct ctttgagata tttttcattt tcagtcagat attccttgag tcgttcaagc
     5041 tcatccgccg gctttgtcga agattgcagg ctacgaagct ggctggctcc tatagcggtg
     5101 agattcgcca gctcttcacg gtcggcatcg ccgcccaaca acagaagaag acggtcaata
     5161 tacggcttgc cgcgaaaaac aaccgccaca tcaatctgct tcatccgcag attgtaataa
     5221 tttataagcg cctcttcttt ttcatcctcg ttctgaatgc cgtcaagacg gtcttcgata
     5281 tcttttatat tctgccgcat caattcggcg gcttgcgcga gatatttttc acgcagtgca
     5341 ggatcctggg cgttgtcggc tttcgtaaga gccgccgata tccggaggtt gatgttatcc
     5401 gtccgctcgg cgagagcttc aagcagttcg ctcatccgga gttttgtaag aacgtccgac
     5461 aaagcctcat cgtcaaggcg ttcataagcg cttttacgct cctgggccaa cgaaacggac
     5521 gacgcgacaa aaatcgccgc caacattaac ataaccaaat gacgtcctgt cgtcattgta
     5581 actccttagc tcctgctttc attgattcgt cattacaccg taaccaggcc ggctgactca
     5641 tcccgcaccg tcgccggaaa acgctatctt tttgaattgt gcgcgaacag cggcgttaaa
     5701 cgcttcaact acaaatttcc acctggcaaa gccgtcgctt tttatcaata ccggaatttc
     5761 agccggatcg ccggtttttg cgcggctttc cagataggcg tagagcattt ccggcttatc
     5821 cgtcggcgga ttgttatcga ctgtatatac gacctggtcc cgcaggctgc cctgcggcat
     5881 tatgatgatg cgtatcggcc gaaacggcgg cgcctccgat gccgctccgc cggcagccgg
     5941 caacgacgcc ggtatctggc cttcatcctt gcgaaacgta ctggtcaaaa ggaagtaaag
     6001 cagaagttca aacgtcacgt caatcatcgg cgtaaggggc ggctgcatgc tctttttgtc
     6061 catctgccgc ttctgccgag gcgcaacgta atgctgctct tccgaacctt cctctatctg
     6121 catgatttcg tttataggca tttcgttcct cgttaatctt atcaacgaca aaacacgtat
     6181 cgcgggcagt tttaccgctt caccctatgc tctggatcga ctagcgcggt catgctcatt
     6241 ttgtttattt tcgcttcagc ggcagcccgc atgacaggct ctatatcctg aaagacgatg
     6301 tccttgtcgg cacgtatttc aacgattatg tcgccttcct tcacattttt gatattcttg
     6361 tccgacagaa tctgggcttt tctttccttt atcgcagctg tcaatgcctc gatattgccg
     6421 gcatctatct tattctggcc aagctggtaa cattcagcct tccctgcgag ttccatctga
     6481 cgattatcat cgctatcgcc gtatttgttc acgacgttta cggttacatt attcagattc
     6541 gtcggctggt caggtttgtc gggatcggcg cctatcgcca ttgatttaac cggctcaggc
     6601 agagagacct tggcaagttc ctggctggcc atctgcgcgg taaggatgaa aaatatgatt
     6661 aactggaaag tacagtcaat catgggcgtc agattcactt cctgtatcga gccggtaatt
     6721 tgttttttag ccatagtgta tctccaaaac aatctttcag cagacaaagc agctcatata
     6781 acgccggctt tgtcgataac accagccggc attactggcg tttggcttcc gggcggaatg
     6841 cggatatcat ttcctgtgcg accttaacgg actcgctcgt aagagcgtct atacgggttc
     6901 ggagcatgga gtaaaggctc aacgcaggaa tggaaaccgt caatcccaga agggtcgtaa
     6961 caagagcgat accaatggcg cctgcaaggt ctttgggatt cggggcgccg gcttcaacta
     7021 tctggaagaa cgcaagaatc atgccccaaa ccgtaccaag cagaccaagc ataggcccga
     7081 tattgcccat aaggctcagc cattcgaggt tacgcagcag tttggtggta cgttcttcgg
     7141 cggcttcttc catggcacgc tccatcgccg gaaagccgta aggcgcttcc ttcaacgatg
     7201 aatgaactat atagctgaga aaatcgctct gctcagccgt aatgtcgatg gcttcacgat
     7261 attgtttggc ggcaaacagc tcattaagct ggtcacgcac cgcatcggga gctatgttta
     7321 cgcggcgaat gctcataaaa tagtgaatcg caagcgcaac cacggcaacg ctcattgccc
     7381 ataggatata acctatcgta ccgccaccaa caacaaggat gtcaaagtaa ttcatgatat
     7441 ctccttatag cttacatcct gagtcgccac accggcgaca tgtcaaacaa aatatttttt
     7501 tacatcagcc gaccatacgg ctgcgactta tcagtcatct tttgcctggt tgagagcctg
     7561 caattcctgc tgagctttct ttacccacgg attgatagcc tggccttcct cggcataatg
     7621 ctcagccaac tcggtcagcg ccgcacgtgc gccggcagtc tggcccaaca attcgttgac
     7681 ttttgcgccg tagaacagcg cttcggggcc gctttcactg tcgttatatt cagcatagac
     7741 tctcatcaga ttaaggcctg agtctataag caggcctttg tcgttattca tgcgtttggc
     7801 cttttccatt tgcgcgcggc ccaacagggt aagcgcagcc ggaagctttt catcgtcata
     7861 cttgttgagg ttttccttga tggaatctat gacgacatca tatttgccgg cgttataaag
     7921 ctgtcccaac accagcaggt cggcagtttt accggctgcg agcggtgcag ccggcgtgga
     7981 agacgacggc cttcgggcat cagaagtttt tgaagaattt tcatccgagt cggcagcaga
     8041 tgagtctgcc ggcgtaaccg gatgctcgcc ggtaagatcc gcggaatcga tattgctcaa
     8101 aatgacggca gtcctggctg cgccgtcatt gtcgcccatt gcctgctgta ttttcatttt
     8161 cgtcttcagc aacgcctgga tgaatttgcg attcgttttt ctgtcggctg ccagtcgctt
     8221 aatctggctg ttgataacat caacggcgga tttattcaca ctttcatttc caggattagc
     8281 cggcgacaaa gcaagtatcg ccgaacatga ttcaaaagtt tcgacgagtt ttatccacgc
     8341 ttttacggcg atatcgatgc gtttagacct ggcgacggcc tgatagcggc ggatttctat
     8401 aagttgtttc atccagtcgg agttggctgc tttgagagcc gcgccgtatg acttgacagc
     8461 tgtgccgaga tcgcctgcag ccagagcctt ttccgccttg ttaaatgcgt cgtcgccgtt
     8521 aatcgtcatg gacgcaatct ccgccaccgg ctgctccaga gtattagccc tcagtctgac
     8581 agtaatttca aaactctgcg ccgaaaaatc ctttattttg ccttcaaacg ctttaccatc
     8641 attgaggttt atcatgtcag ccgtagcgac ggctgaaagt agaagcaata aaatcgggca
     8701 aatggctgct attttccgca tcagtcaccc cactcgatat atttgtactg gcctttgttc
     8761 tgctcggcaa tctgctgcat gacctggatg gctgcttttt ctttcgaacc gaataacagc
     8821 gtatttatgt tcactttttt attggagtta agagcccgga tggcagccag cactttgtcg
     8881 ttgctaacac cttcgccctt gaactcaccg tctgaaagca gatataccag ccggccaggc
     8941 ttgacggctc cgcgaagcac ctggaaagcc ctgtccaatg cgggaatcgg gttactaagg
     9001 ccagacccgc tgtagcccga aaggaaatca gccgcctttt tcttgttttt tttgtttgcc
     9061 agcataaggc tgcgtgagca ttcctccggc ttgccggcag tataaagaat gacggcaaaa
     9121 gtctgcttgt cgctcaggcg agctattgac cgatacattt cgtcacaaac cgccttgaac
     9181 gcacctgaaa tttccatcga gccggaaccg tctattacat agacaacgtt ataggcattg
     9241 ccgccgccca agccgaaaaa cctgttgcca ggcccgcccc cactactcgt agccatgccc
     9301 atgtcagcac ccgcactgcc agccgatgcg ccctcgacac caataacgga aatggcgttc
     9361 ttcgtcgcgc cggtatccgt actgaccttg gttatct
//
LOCUS       DAONFI010000049         9390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8394_length_9390_cov_3.658275, whole genome shotgun sequence.
ACCESSION   DAONFI010000049 DAONFI010000000
VERSION     DAONFI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8394_length_9390_cov_3.658275"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLK08_02595"
     CDS             <1..212
                     /locus_tag="PLK08_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52417.1"
                     /translation="GESLEIQLMESSSKKVNLIELTGLKDVTKVTVNGKLCKVSEVDG
                     KLVIAGPFEKISPNPDNPDLFWIVK"
     gene            235..990
                     /locus_tag="PLK08_02600"
     CDS             235..990
                     /locus_tag="PLK08_02600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52418.1"
                     /translation="MKLRRSILTNLHSAKIQRPGKSRLAGCPQVFCGFTLIELLVVIA
                     IISLLVSILMPSLNKAKDLARQVTCSANIKNINLGFNLYINDWDGCFPFVRETLTPTG
                     TWADKLYTDYINDVNVFMCPSVPERTFTPNVSGGSTLMAYGMEWWLGGGYNITESTLM
                     TESQWKARQLISIAKPSVTVLLGENDNSSGVHGYGVHNAQWPVPFYEWGWPDDERHSG
                     VSNILFVDGHVFAYTQDDACKSENGLIWFYNGD"
     gene            complement(1121..2254)
                     /locus_tag="PLK08_02605"
     CDS             complement(1121..2254)
                     /locus_tag="PLK08_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPS52419.1"
                     /translation="MEIRTALLTAIVSASFAFGGTADKHTAQPTKEKPQPLIEVAFVL
                     DTTGSMSGLIDGAKKKIWEIANQIVCGKPTPKVRMALIGYRDKGDEYVTKMFDLTDNI
                     DKVYENLQTFAAGGGGDSPEHVNKALQDAIYSLKWTSDSENPNVARIIYLVGDYPPHN
                     DYTDTPTSDKLAKAAIEKGICINTVLCGSNQQTRNDWQQIASTAEGAFFAISQNGGME
                     DIATPYDVKLAEKNKELMSTVVVYGSLSEQAVAKEMNAKACKIAAPSVASARASYMGK
                     QKSVGTADLVGALADKKVEMSKLDKDQLPDELKGKTPEQQEAYLKQQAEKRTAIQAEI
                     NTLNRQRNDYIAQQKKDKNIKSEFDENVLKALKKQAARAGVSY"
     gene            complement(2281..4560)
                     /locus_tag="PLK08_02610"
     CDS             complement(2281..4560)
                     /locus_tag="PLK08_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPS52420.1"
                     /translation="MKNRTFEITAGLFFLAISLVPAAGQGILVPVDPTRAISNQYVIN
                     SLTADIQVRGQLMSVSTEQEFENISGGLLEAEFFLPVPPDTAVDSMTLLVNGKEFSAK
                     LLKADEARKIYEEIVRKRKDPALLEYAGYNLFRTRAFPLERGKPAKIQITYKTLCRKD
                     GNMVEVWYPLNSGKYSSRNIGQIKIDIDIKADHSVAGIYSPTHELSISRKADNNVIAT
                     YDRKNIVPVEDIQLFFEQPADVIGSGMITYVPKDENQKGYFLMLLSPNPTQEDKPQPK
                     DVVLVLDRSGSMGGDKIRQAKAAFNFVLNHLNPADRFNVVIYSDSVEVLFDKMLDVSK
                     ENIARAAAEIDAVTARGGTNIYDAVAAALAAMQPSPDALRPGYVMFLTDGLPTVGAST
                     DTQTIIKHAVEANKSNARIFAFGVGYDVNVPLLDKLTDGNHGRSSYVRPNQSVEAIIA
                     SLYNKIRNPVMTNLSVTLKDVAISEMYPAAPGDLFAGDQIVIAGRFSLADAQKLSHGQ
                     SQIIVKGNLRGSEQTFEFPVVMNLNAARANEFIERLWAIRRVGYLMDQVQLHGKTKEV
                     EDEIIALATKYGIMTPYTSFLADESTNLADRPAVMLRVREKLYSLKAAKTKADAQQAA
                     GMRNSLNLAQQVTAAEAAKSTSTPDSLSTPAAPTSKMHGNSSIADFEADKKEAVANVR
                     QIRSQNLYKRGNMWIAANAQHLDIEKDAAKIQHVKRFSDEYFRMIKENTQDENAVLAT
                     QQAGEQILICLRGQAYIIE"
     gene            complement(4985..5356)
                     /gene="rplT"
                     /locus_tag="PLK08_02615"
     CDS             complement(4985..5356)
                     /gene="rplT"
                     /locus_tag="PLK08_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPS52421.1"
                     /translation="MPRVRKGSARTQKHKRILKAASGYHGANSRRYRVAAGKVFRSGC
                     MATKARRIRKRDFRSLWITRISAACRQRGIRYSQLIAAMAEKLIMLNRKMLSEIAIAE
                     PAAFDAICVAALGKEAIKKPE"
     gene            complement(5544..5738)
                     /locus_tag="PLK08_02620"
     CDS             complement(5544..5738)
                     /locus_tag="PLK08_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HPS52422.1"
                     /translation="MPKQKTNKAVAKRIKVTGTGKMKHYSPGSGHLKSRKSNKILRNF
                     RKAKDLSPTFARMAKKMLHI"
     gene            complement(5923..6585)
                     /gene="infC"
                     /locus_tag="PLK08_02625"
     CDS             complement(5923..6585)
                     /gene="infC"
                     /locus_tag="PLK08_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPS52423.1"
                     /translation="MKKFLRSTAISKDLRTNHRIRISPIRLIDQNDKQIGIVETRDAI
                     RMAMEVGYDLVEVAPMAKPPVCRIMDFGKYKYALNKKQQKAKAHRHETELKEVRIRTP
                     KIGAHDLEIKLSQARKFLDRGDRVQFSLRFRGRELAHTDEGMAIFEQIRKALGDVAKV
                     DQPAKFEGKRITMMLATLGKQDQRKHSENDEDESSSILPDIDFDEDILDDDDEDNGDD
                     ED"
     gene            complement(6686..8680)
                     /gene="thrS"
                     /locus_tag="PLK08_02630"
     CDS             complement(6686..8680)
                     /gene="thrS"
                     /locus_tag="PLK08_02630"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPS52424.1"
                     /translation="MVKVKLPDGSVKEYQQSISPLAVAGDISQGLARAAIAARVDGKL
                     VDLTFNMADGEHELQLLTNRDKESLEILRHTAAHVLAMAVVRLYGPTVQYTIGPALLD
                     DFQYGFYYDFALPCNISVEDLSKIEAEMKKIVSEKLPFRREELSAAAAKSLLGKRGQK
                     YKCEMIDDMLAAGEGAVVSLYHQGEGPDAFVDMCRGPHLSDTGKLGNAFKLMAVAGAY
                     WRGDEKNPMLTRIYGNAFFDKKELAEHLDRIEQAKKRDHRVLGKQLSLFLLDEKVGPG
                     MAMWLPRGATIRMELENWLRGELIKGGYSIVYSPHIGKLDLYRTSGHYPYYKESQFPP
                     IMFEEDGKEDGFLLKPMNCPHHIMMYKSTQHSYRELPIRLAEFGQVYRWEQSGELNGL
                     TRVRAFCQDDAHIFCAPEQLESEISACIDLVKLVLSTLGLNDYRVRISLRDPNSDKYV
                     GSDENWNLAEANIRSVVKASGMDYSEALGEAAFYGPKIDFIVKDCIGREWQLGTVQVD
                     YNLPERFELEYIGRDNSAHRPVMIHRAPFGSLERFIGILIEHFGGAFPLWLSPVQVAV
                     LPVSDKVADYAAAVTARLKSAGLRVELDESADKIGAKIRRATMNKIPYMAVIGAKEAD
                     SQTVSVRERIKGDIGEMAVENFKNALVCERDTKGKELVQI"
     gene            9161..>9390
                     /locus_tag="PLK08_02635"
     CDS             9161..>9390
                     /locus_tag="PLK08_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPS52425.1"
                     /translation="MSLLVTGSIGIDTVESPSGIAEDVLGGSSIYFSYAASFFTPVRL
                     VGVVGEDCPQNFLQPLEDNPRIDMGGLEVRKG"
BASE COUNT         2075 a         2247 c         2332 g         2736 t
ORIGIN      
        1 agggtgagtc gctggagata cagttaatgg aaagttcgtc caaaaaagtc aatctaatag
       61 agctaacagg tttgaaagac gttaccaaag taactgtgaa cggcaaacta tgcaaagtat
      121 ccgaagttga cggcaaattg gtaattgcag ggccattcga gaaaatctct cccaaccccg
      181 acaatcctga tttattctgg atagtgaagt aaagattata tatggaggca gattatgaag
      241 ctgcggcgtt ctatattgac gaatctgcat tcggcgaaaa tacaacgccc aggtaagtca
      301 cgacttgccg gatgtccgca agttttttgc gggtttaccc ttatagaatt gttggtagtg
      361 atagcgatta tatcgttatt ggtatcgatt ctgatgccca gtttgaataa agccaaagac
      421 ctggctcgtc aggtaacctg ttctgcaaat atcaagaata tcaatttggg gtttaacctg
      481 tatattaatg attgggatgg atgttttcca tttgtacgcg aaacgctaac acctaccggt
      541 acgtgggctg acaagctgta taccgattac ataaacgacg taaatgtttt tatgtgcccc
      601 agtgttccgg agaggacttt tacgccaaat gtaagcggcg gttcaacgtt gatggcttat
      661 ggtatggaat ggtggctggg tggagggtac aatataaccg aatcgacctt gatgactgaa
      721 tcacaatgga aagcgcgtca actcattagt atcgccaagc catcggtaac tgttttgttg
      781 ggtgaaaatg acaatagctc aggcgtgcat ggatacggtg tgcataacgc tcaatggccg
      841 gttccttttt atgagtgggg ttggccggat gatgaacgtc attcaggtgt atcgaatata
      901 ctttttgtcg atggccatgt gtttgcatat actcaagatg acgcatgtaa gagtgaaaat
      961 ggattaattt ggttttataa tggcgattaa atggtcgttt tggataaaaa caaggctgac
     1021 acatacttgt gtcagcctat acagcttagt atcaccataa cgctagaagt gctttccgac
     1081 agggacaaat cctttctgat tctgaatcat acaactatcc ttaataactt acgcctgctc
     1141 tggcagcctg ctttttgagg gcttttaaaa cattttcatc aaattcgctc ttgatgtttt
     1201 tgtccttttt ctgttgcgcg atataatcat tacgctgccg gtttaacgta tttatttcag
     1261 cctgtatcgc agtgcgtttc tccgcctgct gtttgagata agcttcctgc tgttcaggtg
     1321 ttttgccttt gagttcatcc ggcagttggt ctttgtccag ctttgacatt tcgacctttt
     1381 tgtcggcaag cgcgccgaca aggtcagccg ttccgaccga tttctgtttg cccatgtaac
     1441 tcgctcgtgc tgatgcgacg gatggcgcgg cgattttaca tgccttggcg ttcatctctt
     1501 tggcgacagc ctgttcgctg agtgaaccgt aaaccacgac ggtgctcata agttctttgt
     1561 ttttttccgc cagtttcaca tcgtaagggg tggcgatatc ttccatgccg ccgttttggg
     1621 agatggcgaa aaacgcccct tcagccgtcg atgcaatctg ctgccagtcg ttccgtgtct
     1681 gctgattgct gccgcacagt accgtgttga tgcagatgcc tttttctatc gcagcctttg
     1741 ccagtttatc ggaagttggc gtatcggtgt aatcgttgtg cggcgggtag tcgccgacga
     1801 ggtagattat tcttgcaacg ttcggatttt cactgtcgga tgtccacttc agcgagtaga
     1861 tagcgtcctg cagggctttg ttgacatgtt ccggtgagtc gccgccgccg ccggcagcaa
     1921 atgtctggag attttcgtaa actttgtcga tattgtcagt caaatcgaac atctttgtta
     1981 catattcgtc acccttgtcg cggtagccta tcaacgccat acgaactttt ggcgtgggct
     2041 tgccgcagac tatctgattg gcaatttccc atattttctt tttcgcgccg tcaatgaggc
     2101 cgctcattga gccggtcgtg tcaaggacga acgccacttc aattaacggc tggggttttt
     2161 cttttgtcgg ctgtgctgtg tgcttgtcag ccgtgccgcc gaatgcgaag gatgccgaaa
     2221 cgatggccgt aagtagtgcg gttctgattt ccattttgtt tctccttgtg tttttggggt
     2281 ttattcgatg atgtacgcct ggccgcgcag gcagatgaga atctgctcgc cagcctgctg
     2341 cgtggccaga actgcgtttt cgtcctgcgt attttccttt atcatcctga aatattcatc
     2401 gctgaaacgt ttgacgtgct gaattttagc ggcatctttt tcgatatcga gatgttgcgc
     2461 gtttgccgct atccacatat taccgcgttt atagagattt tgcgaacgaa tctggcggac
     2521 gttggcgacg gcttcttttt tgtcggcttc aaaatctgcg atgctggagt tgccgtgcat
     2581 ttttgacgtt ggcgcggctg gggtagataa agaatccggg gttgatgtgg actttgccgc
     2641 ttccgctgcg gttacctgtt gtgccaggtt gagagaattt ctcataccgg cggcttgttg
     2701 ggcatcggct ttggtttttg cggctttgag gctataaagt ttttccctga ctcgcaacat
     2761 gactgccggc cggtctgcaa gattcgtgct ttcatcagcc agaaaactgg tgtatggagt
     2821 cattattcca tatttcgtgg ctaatgcgat tatttcgtct tcgacctcct tggtcttgcc
     2881 gtgcaactgt acctggtcca tgagatatcc cacgcgccgt atcgcccaga gacgttcaat
     2941 gaattcattg gctctcgcgg cgttcagatt catgactacc ggaaattcga atgtctgttc
     3001 actgccgcga agattgccct tgacgattat ctgcgattgg ccgtgactga gtttttgcgc
     3061 gtcagccagg ctgaatctgc cggcaatgac tatctggtcg ccggcgaaca gatcgccagg
     3121 tgctgcggga tacatttcgg aaatcgctac gtctttgagc gttactgaca gattggtcat
     3181 gacgggattg cgtatcttgt tataaagcga ggcgattatg gcttcaaccg attgattggg
     3241 cctgacgtat gaacttctgc cgtgatttcc gtccgtgagt ttgtccaaaa gcggaacgtt
     3301 aacgtcgtaa cctacgccga atgcgaatat gcgggcgttg gatttgtttg cctcgacggc
     3361 atgtttgatt atagtctgtg tgtcggtact tgcgcctact gttggcagtc cgtccgtaag
     3421 gaacatgacg taacctggac gtaatgcgtc aggtgagggt tgcatcgctg ccagtgcggc
     3481 ggcgacggcg tcgtagatgt tagtgccgcc gcgagcggtg acggcgtcta tttctgcggc
     3541 agctctggcg atattttctt tactgacgtc gagcattttg tcgaacagca cctcgaccga
     3601 atcggaataa atcaccacat tgaagcgatc cgccggattg aggtgattta atacgaaatt
     3661 aaacgcagcc ttcgcctgcc ggattttatc gccacccata gagccggaac gatccagcac
     3721 aagaaccaca tctttcggct ggggtttgtc ttcctgtgtc gggttcgggc ttaacagcat
     3781 aagaaaataa cctttctggt tttcgtcttt tgggacatag gttatcatgc ccgaacctat
     3841 tacgtcagcc ggctgttcga agaagagctg tatgtcttcg acggggacta tgtttttgcg
     3901 gtcgtaagtg gctattacgt tgttgtcggc ttttcgcgat attgaaagct catgcgttgg
     3961 cgaatatatg cctgcgacgc tatggtcggc tttgatgtcg atgtcaatct tgatttggcc
     4021 gatgtttcgc gacgaatatt tgccgctgtt cagcggatac cagacctcga ccatattgcc
     4081 gtctttccgg cagagagttt tgtatgttat ctggatttta gccggtttgc cacgttcgag
     4141 cggaaaggct cgcgtacgaa acagattgta gccggcgtat tccagcaggg cgggatcttt
     4201 gcgtttgcgg acgatttcct cgtaaatttt gcgagcttcg tcagccttga ggagtttggc
     4261 cgaaaattct ttgccgttga ccagcagcgt cattgaatcc acggcggtat ccggcggaac
     4321 gggcagaaaa aattccgctt cgagcaggcc gccggagatg ttttcaaact cctgctctgt
     4381 cgatacgctc atgagttgcc ctcttacctg aatatccgcc gtcaatgagt ttatgacgta
     4441 ttgattggat atcgcccgcg tcgggtcgac gggcacaagt atcccctggc cggctgccgg
     4501 caccagggat attgccagaa aaaataaccc agccgtgatt tcaaacgtgc ggtttttcat
     4561 cttcaatctc ctgaaagtga ccagaattcg aaaattatct tttacctttt ccggatttct
     4621 gaattcacca cttttgatgg acatgtaaga caaaagttcc cttgaaattt ttttttaccg
     4681 gtaaaaattt ttaaactgcc cgatttgatg gtttttcatg tatcacgcca aaaggtaaaa
     4741 acttgtttta aaattaagat acatcacggt ttttgccaac ggcaataccg caaaagacgt
     4801 ttatcctgta tatgataact gcgtataaaa atttttttaa aaactttgtc aaatcctgga
     4861 acgcgaatcg gtcattttga gaaaaaaacg caagctaaag ttgagatgcc ataaaaaaag
     4921 tccagcggat tgggctggac ttttcagaag aatttttgac aaaattctga ttgttacgtg
     4981 taaattactc aggttttttt atagcctctt tgccgagagc cgcaacgcag attgcgtcga
     5041 aggcagccgg ctcggcgatg gcgatttcgc tgagcatttt gcggttaagc atgataagtt
     5101 tttcagccat tgctgcaatc agttggctgt agcggattcc tcgctggcgg caggctgccg
     5161 aaatgcgtgt aatccacagg ctgcggaagt cacgtttccg gatgcggcga gccttggtgg
     5221 ccatgcatcc cgaacggaag accttgccgg ccgctacgcg atagcggcgc gaattggcgc
     5281 catgataacc gctggcggct ttgaggattc ttttgtgctt ctgcgtacgt gctgaacctt
     5341 tacgtactcg tggcatgata atatcctttc gttactgtcg ggcggaccct cgtttttgag
     5401 gtgtttctgc ctgccgctgc cttcggcacg ccgtcccatc ggactcccgc caaaccttga
     5461 tttacttttt ccatcaccat ccagtacgcc tgaatccgcg gcagcagggc gtaactgaaa
     5521 ccgtctcaca aggctgcttc acctcagatg tgaagcattt tctttgccat tcgtgcgaaa
     5581 gtcggggaaa ggtccttcgc tttgcggaaa ttgcgaagaa tcttgttgga cttgcggctt
     5641 ttaagatggc ctgaacccgg gctgtaatgt ttcatcttac ccgtgccggt caccttgata
     5701 cgcttggcaa cagccttgtt agttttctgc ttgggcattt tatgctcctg tcacgcagac
     5761 aaaacaacat taactccgta ccgcatacgt gggtacgaac accatatctt tttccacatt
     5821 tagcggaaag actcaaaatt ctaacgattt gacgccggtt gtaaaggttt ttatgtcgct
     5881 gaatcgaaaa tatttatctt cgattctctg cagggcagaa cttcaatctt catcatcgcc
     5941 gttatcttcg tcatcgtcgt ccagaatatc ttcgtcaaaa tcgatatccg gaagaatcga
     6001 cgatgattca tcttcgtcgt tttcggaatg tttgcgttgg tcctgcttgc ctagcgtcgc
     6061 cagcatcatg gtgattcttt tgccttcaaa cttagccggc tgatcaactt ttgccacgtc
     6121 gccgagggct tttcttatct gttcgaatat cgccatgccc tcatcggtgt gcgccagctc
     6181 tctgccgcga aaacgcaggg aaaactgaac cctgtcgccg cggtcaagga acttgcgagc
     6241 ctggctgagt tttatttcca ggtcgtgagc tccaatcttg ggcgtgcgga tgcgaacttc
     6301 cttaagttcg gtttcatgcc ggtgggcttt ggctttctgc tgctttttat tcagcgcgta
     6361 tttatatttg ccaaaatcca taatccggca gaccggcggc ttagccatcg gagcgacttc
     6421 aacaaggtca taaccgactt ccatcgccat gcggatggca tcgcgggttt cgacgattcc
     6481 tatctgtttg tcgttctggt ctatcagcct tatgggcgat atccttatcc gatggttcgt
     6541 tctaagatcc ttagagatag cagtactcct taaaaatttt ttcaaacacc tgtttacacg
     6601 tccgacagac tggataattt aaaaaatccg ttctgaattt ccaaacgcaa atttgcaatt
     6661 tttttcactt ttcagttaaa aatttctaaa tttgtaccag ttccttgcct tttgtatccc
     6721 gttcgcagac tagggcgttt ttgaaatttt ctacagccat ttcgccgatg tcgcctttga
     6781 ttcgttcgcg gacagatacg gtttgtgagt cggcttcttt agcaccgatg acagccatgt
     6841 atggtatttt gttcattgtt gccctgcgga tttttgcgcc tatcttatcg gcagattcgt
     6901 cgagttcgac ccgcaagccg gctgatttca acctggctgt aacagcggcg gcgtaatcgg
     6961 cgaccttgtc gctcacgggc agtacagcca cctgcaccgg actcagccac agcggaaacg
     7021 ctccgccgaa atgctcaatc agaatgccga tgaatctctc aagcgacccg aacggcgcac
     7081 gatgaatcat gaccggccga tgtgccgaat tatccctgcc gatgtattca agttcgaatc
     7141 gttcgggcag attatagtca acctggacag tgccgagctg ccattcacgg ccgatgcagt
     7201 ccttgacgat aaaatcgatt ttcggcccgt aaaaggcggc ttcaccgagg gcttcactgt
     7261 aatccatgcc agatgccttg actacgctgc gaatgttcgc ctcggcgaga ttccagtttt
     7321 catcgctgcc gacgtacttg tcgctatttg ggtcgcgaag tgaaattcgt acgcggtaat
     7381 cgttcaggcc cagtgtactg agtaccagct ttaccaggtc gatgcaggcg gatatttcgc
     7441 tttcgagctg ttccggcgca cagaatatgt gggcgtcgtc ctggcagaac gcgcgtactc
     7501 tcgtcaagcc gttaagctcg cccgactgct cccagcggta tacctggccg aattccgcca
     7561 gccgtatcgg cagttcgcga tagctgtgct gggtcgactt atacatcatg atgtgatgcg
     7621 ggcagttcat gggttttaga aggaaaccgt cttccttgcc gtcttcttca aacattatcg
     7681 gcgggaattg cgattccttg taataaggat aatggccgct tgttcgatac aggtccagct
     7741 tgccgatgtg cggcgaatat acgatggaat acccgccctt tatgagttcg cctcgcagcc
     7801 agttttccag ttccatgcgt attgttgcgc cgcgcggcag ccacatggcc atgcccggcc
     7861 cgactttttc gtccagcaga aaaaggctga gctgcttgcc cagcacgcgg tggtcgcgtt
     7921 tttttgcctg ctcgattcgg tccagatgct ccgccagttc ctttttgtca aaaaaagcat
     7981 tgccgtaaat gcgcgtaagc atggggtttt tctcatcgcc gcgccaatat gcaccggcga
     8041 cagccatgag tttgaacgcg ttgcctagtt taccggtatc gctcagatgc gggccgcggc
     8101 acatgtccac gaatgcgtcg gggccttcgc cctggtgata aaggctgact actgcgcctt
     8161 cgccggcggc aagcatgtcg tctatcattt cacatttata cttctgaccg cgtttgccca
     8221 gcagagactt tgcggcggca gcggaaagct cttcacggcg aaacggaagt ttttcggaaa
     8281 cgattttttt catctcggct tctattttgg acaaatcttc cacgctgatg ttgcacggca
     8341 gggcgaagtc gtaataaaaa ccgtattgga aatcgtctaa aagggccggc ccgatagtgt
     8401 attgcaccgt tggcccatac agacgcacca ccgccatcgc cagcacgtga gccgccgtgt
     8461 gtcgcagtat ttcgagcgat tccttgtcgc gattggtgag caactgaagc tcatgttcgc
     8521 cgtcagccat gttgaacgtc aaatcaacca gtttgccgtc gacccttgcc gctatcgccg
     8581 cacgggccaa accttgcgaa atgtcaccgg caaccgccag cggacttatc gactgctggt
     8641 attcttttac gcttccgtca ggtaatttta ccttaaccac tttaaatgtc ctcaaaaacc
     8701 aaaatgcgaa tttttataac ccaaaacaga ctcgagttaa aaattcaaaa gaaatttttt
     8761 taaaaaaaca acaacctgat tccgtgattc actttttaac tgcttaactg tgtacaaaag
     8821 aaaaccggat gcactcgtga atttgcacac acttaaaacc tcctgccgca ttgtttatgc
     8881 cgcaggccgt caaaacgtag tcgttatctg tgtgttcata ttcatgaccg catccggttt
     8941 catttagtat agttaagaat atccgtgttt tatcggcatt tcaacacaaa tctatatgaa
     9001 tccggctgat tatttagagt gttttcgctg tggcaacctt aaaattgagt cgtgaatccg
     9061 gaaaactgtt ttccacgcgg tgaggaatcc ttataatgag ccgttattgt ggcttatgcc
     9121 ggtgttgaag tctggttgtt taaaaggaaa ggttttgaga atgagtcttc ttgttacggg
     9181 cagtattggt attgacaccg tcgaatcgcc gtccggcatt gccgaagatg ttttgggcgg
     9241 ttcgagcata tatttttcat acgccgccag tttttttacg ccggtgcggc tggttggcgt
     9301 agtcggcgaa gactgccccc aaaattttct ccagccactt gaagataacc cgcgcatcga
     9361 catgggcggc cttgaagtgc gaaagggcag
//
LOCUS       DAONFI010000050         9331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8458_length_9331_cov_4.113519, whole genome shotgun sequence.
ACCESSION   DAONFI010000050 DAONFI010000000
VERSION     DAONFI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8458_length_9331_cov_4.113519"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLK08_02640"
     CDS             complement(<1..823)
                     /locus_tag="PLK08_02640"
                     /inference="COORDINATES: protein motif:HMM:NF026044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52426.1"
                     /translation="MENDRKKHGFDWLTATLLAWALPGMGHVFLGRAKRGIIICVTIT
                     ALFWSGIAVGGVMTIDSRYEKWWFYAQILNGASGVISWLRSEREFREIEKTPEMQQLI
                     ADTLYMRAGMGFKTEVDPADNSMTGALADRHIRENVAMTRKMRVTIPVIFIGEAVGVT
                     QGGGILEHVLRELEVECLPGDLVDSIPVDITQLALGGRLLVRDMQIPAKITVLTSGDQ
                     PVAIVSIPKEEEVVAPEATDAAATEPELIKKKKEEEGEEAAEGDKKGAKKPDEKKP"
     gene            complement(878..2818)
                     /locus_tag="PLK08_02645"
     CDS             complement(878..2818)
                     /locus_tag="PLK08_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS52427.1"
                     /translation="MSFLTIFRANNTGQFQAVAVTAVIVLTFICTSTKAAGEKPLPQP
                     QIDASTLSHSARFGGDRTVELLGDILLKSEDVYERSQAAMNLGQTNNMNAVKFLVKGS
                     GDKEPQVRISVITAAAALAQQTPDYMRPNSEEYQTTVDIIRKGFADSSESVRVAAISS
                     AAAANVLENKIIKAAAEDDQLPAMQAIIALNRQHRGIKNADSILKHLSSDNLCLQIVA
                     AENLIYADSDNSAESSQHDTTQLIATLKKLSQSGNDALAATAIVSLTALNSAAAKPFI
                     EKAAKSNSPLMRRAAVKSFAIYHELKAVAKNAEKPATEHGSIPTSTDIRLYDRISPYL
                     DDASPLVQLAAVNAAEVARKNGVLLNQTQKLIDIAFNAPDTQLHNAAMTAAAVADGEI
                     FTKIIAAAYGKMMPEYLASKNPAGSILERNLSGVCRMLGRMKSAAVFGQMLQDIEKLP
                     IDSIIIGDIADSLANTGDKAADEVLERMLKKCHATGAKYLIAIASMAEPPAFSEDVTA
                     KIINALASLDDKQATDEIIKISVMNIMGMKLTTVSAASADALAKLYDDTNRTAIDKLL
                     IEMIADKSYGNIAMLNAIIAAGRLKLPDAVKNLKIVLNDERPSKIAIWAAAWAIGQIS
                     GQYPPQPQPVQNQGDWLIKSTK"
     gene            complement(2838..4406)
                     /locus_tag="PLK08_02650"
     CDS             complement(2838..4406)
                     /locus_tag="PLK08_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPS52428.1"
                     /translation="MQSIGGQESRRLLSVLSIGQLVMIILFAPAFTAASLTGEKERNT
                     MESLYASKLRPIEIVVGKMAGSLCFLLLTVLTGTVALVMPLLLGGVEWQEVAAITGIN
                     FMSAIFLGMLGLLVSTIMQRSYRAIICTYILLLIVCFAFALPAWPIGQGASLLKRSDP
                     ITQMFLHFLASLSPIEAMLSRVWPGNELVQGVKTLPPMWLVFLPLSIIMSLGIAVLCI
                     LKLRKPFKPPRPREKLAVVERNEVRASSVILYLWFFDPRRRRKNIAWWQNPVLMKEFR
                     TRPMLQTRWLLRAFGICLITSVLLMILVSISISQFVEESTNMTNMMATAVAVMVVVLV
                     VLIGPAIAGGTICSDIETGVWDLLRISPMRSWQIVSGKFQASIIPLILLAVATLPAMF
                     ILLVFDVTLLPNLLRICAVVGVTIIFVTTAGTFFSSIFRRTAVATAWAYGLVVSITLL
                     TLLVLLAKDLFTQKFISDVFLINPIVAAMDAAGNVEMQKMGLLRLHIQIMCTATAVLL
                     AVTVLRVFQLRKAD"
     gene            complement(4394..4531)
                     /locus_tag="PLK08_02655"
     CDS             complement(4394..4531)
                     /locus_tag="PLK08_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52429.1"
                     /translation="MNNPIVHKEVLTSLRTKKAFWMQAMFFSSQPYLYGCFGRLTVCS
                     P"
     gene            complement(4524..5609)
                     /locus_tag="PLK08_02660"
     CDS             complement(4524..5609)
                     /locus_tag="PLK08_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS52430.1"
                     /translation="MSSESVLVCEKLRKAYDGFVALEGLDLEIPAGSVFGYIGHNGAG
                     KTTTIRMLAGLLLPSDGRASICGYDVVKDRNQIKSLLGYMPDNFGVYDQMRVWEYLDF
                     FGAAYKIPRKERIRRLDYVLEMTEGEYMRDRFVDTLSKGMKQRVGIARTLMHDPKVIL
                     LDEPANGLDPRARVQMRELIRKLADNGKTILVSSHILPELAAICNLVGILHRGRLLAS
                     GTIDDVLKNLHRDRLMEAKLKGDITAAQDVLKNDAKAAEIQVSEIDSNMLQFRYSGDD
                     DSLADLLAAINNAGSRVVTFREVPLSLEDAYMTISAMGLEGDETADDAVPATAEESQN
                     KGILQEGQCAGCCKCDDIGSCEEHADE"
     gene            complement(5609..8203)
                     /locus_tag="PLK08_02665"
     CDS             complement(5609..8203)
                     /locus_tag="PLK08_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52431.1"
                     /translation="MAANRFRLSAVLAKFLCLAVFLAVASTALCQTESVTLKSIRGPF
                     HPFSSERTWTQKICEVQNDASRNLSLSVKTTATDDKLGRLEFRRDFTSPANSIRQVTV
                     GMRFGSFARIAAGSRHGQTKSGMSKFGQTFIIQETGNERQLSLQESILLGVPDKTLHT
                     AVVNGDNGDSFAYMKDDGSNPLGNILFISAGNVENLPTNWYGYELLDMLMVGPVDDSK
                     LLPSQFTAMEQWVRKGGRLLIFCGEGSFEQLQGRLGRLAGVHALGFHHTDRLNVDGQT
                     YTLAKPTYFAELAADGATVLETCDTLPLLTAKRTGMGVVMVAACSLQALQPQGNFNVW
                     KNVAVQVHSRQALDPANFSNVVSVKETVKVDKQAEPVSKNKATSKKFSRHRPASPRYV
                     DKTRNVIPGFATLQEIAGRQAPSSFIPCGILLGFVFATLALGGLLRILRKGELLWIFL
                     IPAVAVMSVVIFQISRHTSDTARLSNVGYVAALGDGQSYIQEFYAFYSGPDASDVDFG
                     SKYMNGIIMPAGENRLANMTTQTIVTGDSQMFMPEKGMKSCATTSFFTETVAAYDPMS
                     ASLSFDSAGATIKIKNNLKQPVNNAVLFSNMLVMKIGNIAPGENIIRMNTADKLAEGE
                     FTSSLMTQDTKWNELISLMLDHGGFHRTITAPRLLGYLDGSAISPLAQTANVKRQGVS
                     VIDIPVTFSPVKSGTEVTIPAALLPSDVMNLEMPVWNTTARKFNSCIRPSKFVYLLGL
                     PENIKKLKKASVELAINLQASGYVMKVSRISLTPGHSDTPRRHFNTVALQVYLSVDNP
                     SGVYNISIPDADKLAVHEGKMAICIELSRLKSDIAQTISAINISGSSNQAMWKIISIT
                     PTLKGTVE"
     gene            complement(8346..8861)
                     /gene="ruvC"
                     /locus_tag="PLK08_02670"
     CDS             complement(8346..8861)
                     /gene="ruvC"
                     /locus_tag="PLK08_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPS52432.1"
                     /translation="MAEEQFNTDAYRVLGIDPGLNITGYAAVEFFSSAEPVVLEAGTI
                     RTDSKADMPRRIDQIFTDLQEIIAEIRPNVVAIEQLYSHYAHPRTSILMGHARGVIML
                     AAAHAGILVHSVSATRVKKSLTGNGHASKQQMQLAIQSLLSLENPPEPPDVADALAIA
                     LCAGREVHLNV"
     gene            9143..>9331
                     /locus_tag="PLK08_02675"
     CDS             9143..>9331
                     /locus_tag="PLK08_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52433.1"
                     /translation="MKRQQFFEIDGFDTALADSLKASHEPLRPDFTNKVMQRRNKIIE
                     AEFLAKSIRQERLALIACI"
BASE COUNT         2000 a         2415 c         2466 g         2450 t
ORIGIN      
        1 ccggcttctt ctcgtccggc ttcttggcgc ccttcttgtc gccttccgcc gcctcttcgc
       61 cttcctcttc cttcttcttc ttgatcaatt ccggctcggt cgcagccgcg tcggtcgcct
      121 ccggcgccac cacctcttcc tccttcggaa tcgacacaat cgccaccggt tggtcacccg
      181 acgtcagaac ggttattttc gccggaatct gcatatcccg caccaacagg cgtccaccca
      241 gggcgagctg ggtaatatcc accggaatcg aatccaccag gtcacccggc agacactcga
      301 cttcgagttc acgcaacaca tgctccagaa taccgccgcc ctgcgtcacc ccaacggcct
      361 cgccgatgaa gatcaccgga atggtcacgc gcatcttgcg ggtcatcgcc acattctcgc
      421 ggatgtgccg gtcggccagc gcgccggtca tgctgttgtc ggcagggtcc acctcggtct
      481 tgaagcccat gccggctctc atgtagagcg tatctgcgat aagctgctgc atttccggcg
      541 ttttttcaat ttcacggaat tctctctccg agcgaagcca gcttattacg ccgcttgcgc
      601 cgttgagaat ctgggcgtaa aaccaccatt tttcataacg gctgtcgata gtcattacgc
      661 cgccgacggc tatgccggac cagaaaagcg cagtaatcgt cacgcagatt attattccgc
      721 gttttgccct gcccaggaaa acatggccca tgcctggcag cgcccacgcc agcaatgttg
      781 ccgtcagcca gtcgaatccg tgtttttttc tgtcgttttc catcgcgtta actataaccg
      841 attcttccac atggttgcaa gcgggacgta ggaagattta ttttgtactt tttatcagcc
      901 agtcgccctg attctgtaca ggttgcggct gtggaggata ttggccggat atctggccga
      961 tagcccatgc ggcggcccat atggcgattt ttgacggccg ttcgtcgtta agcacgattt
     1021 ttaaattttt cacggcgtct ggcagcttta atctgccagc ggcgatgatg gcgttgagca
     1081 tggcgatgtt gccgtaactt ttgtcagcta tcatctctat aagcagtttg tcaattgccg
     1141 tcctgtttgt gtcgtcgtac agctttgcaa gcgcgtcagc cgatgcggcg gatactgtcg
     1201 tgagtttcat tcccatgatg ttcattacag atatttttat tatttcgtca gtcgcctgtt
     1261 tgtcgtccag cgaggccagt gcgttgatta tctttgcggt gacgtcttcg ctgaaggctg
     1321 gcggttcagc catcgatgct attgcgatga ggtatttggc gccggttgcg tggcattttt
     1381 tcaacatgcg ttcgagtact tcatcggctg ctttgtcgcc tgtattagcc agtgagtcgg
     1441 caatatcgcc gattattatc gagtctatcg gcagtttttc gatgtcctgg agcatctgcc
     1501 cgaaaacagc cgctgatttc attctgccga gcattcggca tacgcctgaa agattgcgtt
     1561 ccagaatact gccagccgga ttttttgacg cgagatattc cggcatcatt tttccgtatg
     1621 ccgccgcaat gatttttgtg aaaatttcgc cgtccgctac tgcggcagcc gtcattgcgg
     1681 cgttatgtaa ttgagtatcg ggtgcgttga aggcaatgtc gatgagtttt tgcgtctgat
     1741 tcagcaggac gccgttttta cgggcgactt cagcggcatt tacggcggcg agctgtacca
     1801 gcggtgaggc gtcatccagg tatggcgaga ttctgtcgta aaggcggatg tcggttgagg
     1861 tgggtatcga gccgtgttca gtagccggct tttctgcgtt tttcgcgacg gctttcaatt
     1921 cgtgatatat tgcaaaactt tttactgcgg ctcggcgcat cagaggtgaa ttgcttttgg
     1981 cagctttttc aataaacggt ttggcggcgg ctgaattgag tgcggtcaac gacacaatag
     2041 ctgtcgcggc aagggcgtcg ttaccggatt gcgagagttt tttcaatgtc gcaatcaact
     2101 gtgtcgtgtc atgttgcgaa gattcagccg aattatctga atcggcgtaa atcaaatttt
     2161 cggcggcgac gatttgcagg cacagattgt ctgatgaaag atgttttaat atcgagtcgg
     2221 cattttttat accgcgatgt tggcggttca gggcgattat cgcttgcatt gccggcagtt
     2281 ggtcgtcttc tgcggccgcc tttatgattt tgttctccag aacatttgcc gctgcggcgg
     2341 atgaaattgc cgcgactcgc accgactcgc tggaatccgc aaaccctttt ctgatgatat
     2401 cgactgttgt ttgatattct tctgaattgg ggcgcatata atccggcgtc tgttgggcga
     2461 gggctgcggc ggctgttatc actgaaatcc gaacttgcgg ctctttgtcg cccgaacctt
     2521 tgactagaaa ttttaccgcg ttcatgttat ttgtctggcc gagattcatc gcagcttgcg
     2581 aacgttcata aacgtcttcg cttttaagca ggatatcgcc cagcagttca accgtccggt
     2641 ctcctccgaa gcgggcagaa tggctgaggg ttgatgcgtc gatttgcggc tgcggcagag
     2701 gtttctcgcc ggctgctttc gtcgatgtgc agatgaatgt cagaacaatg acggctgtaa
     2761 ctgcgacagc ctggaattgt ccggtattat ttgcccgaaa aatcgttaaa aaactcattt
     2821 tatgcgtccc gtaaaaatca gtcagccttt ctgagctgga aaacccgcag taccgtcaca
     2881 gccagcagaa cggcggtcgc tgtgcacatt atctgtatgt gcagccgcag caaacccatc
     2941 ttctgcattt cgacgttgcc ggctgcgtcc attgccgcga ctatagggtt tatcagaaaa
     3001 acgtcggaga tgaatttttg cgtaaaaagg tcttttgcca gcagcaccag cagcgtaagc
     3061 agcgttatgc tcactacgag gccgtaagcc catgccgtgg ctacggctgt gcgtctgaat
     3121 atgctggaga aaaaagtgcc cgccgtcgtt acgaaaataa tcgttacgcc taccacggca
     3181 catatccgca gaaggttggg cagcagcgtt acgtcgaaaa ccagcaatat gaacatggcc
     3241 ggcagtgtcg cgacggctaa cagaatcagc ggaattatcg acgcctggaa cttgccgctg
     3301 actatctgcc agcttcgcat gggggagatt ctgagcaaat cccatacgcc ggtttcgatg
     3361 tccgagcata ttgttccgcc tgctatcgcc ggcccgatga gcaccaccag aaccacaacc
     3421 atgaccgcca ctgcggtagc catcatgttc gtcatattcg tggattcctc gacgaactgg
     3481 ctgatagaga tactcacgag aatcatgagc agtaccgacg ttatcaggca tatgccgaat
     3541 gccctcagca gccagcgtgt ctggagcatc ggccgtgtcc ggaattcctt catcagcacg
     3601 ggattctgcc accacgcgat atttttcctt cttcgtcgcg ggtcgaagaa ccacaagtac
     3661 aatatcactg aacttgcgcg gacttcattg cgttcaacga cggcgagttt ttctctcggc
     3721 cgcggcggct tgaatggctt tcgcagtttg agtatgcaaa ggactgctat gcccagcgac
     3781 atgattatgc tcagcggcag aaacaccagc cacattggcg gaagcgtttt tacgccctgt
     3841 acgagttcat tgccaggcca tacgcgggag agcatagcct ctatcgggct cagcgatgcc
     3901 agaaaatgca gaaacatctg cgttatcggg tcgctgcgtt tgaggaggct ggcgccttgg
     3961 cctatcggcc aggccggcag agcgaaggcg aaacatacaa tcaacagcag aatgtacgtg
     4021 cagattatcg cccgataact tcgctgcatg attgtgctga ccagcagccc cagcatgccg
     4081 agaaaaatcg ccgacatgaa atttatgccg gttatcgccg ccacctcctg ccattccact
     4141 ccgcccagca gcagcggcat gacaagtgcg actgtgccgg tcagaaccgt cagcagcaga
     4201 aagcacagcg agccggccat cttgccgacg acgatttcga tcggcctgag tttgctggcg
     4261 tagaggcttt ccatggtgtt gcgttccttt tcgccggtga gcgacgcagc cgtaaacgcc
     4321 ggcgcaaaaa ggattatcat taccagctgg cctatcgaca gtacgctcag cagccggcgg
     4381 gattcctggc cgcctatgga ctgcaaaccg tcagccggcc aaagcagcca tataagtacg
     4441 gctgtgacga aaaaaacatc gcctgcatcc agaatgcttt tttggtgcga agcgaggtaa
     4501 gcacttcctt atgaacaatg gggttattca tcggcgtgtt cctcgcatga gccgatgtcg
     4561 tcacatttgc aacagcctgc gcattggcct tcttgcaaaa tgcctttatt ttgcgattcc
     4621 tctgctgtgg cagggacggc atcgtcggcg gtttcatcgc cttcaaggcc cattgcactt
     4681 atggtcatgt aggcatcttc gaggctcagc ggcacttcgc ggaacgtgac tacccgcgag
     4741 ccggcgttat tgatggcggc gagcaggtcg gcaaggctgt cgtcgtcgcc gctgtagcgg
     4801 aattgaagca tgttcgaatc gatttcagaa acctgaatct cggcagcttt cgcgtcgttt
     4861 ttaaggacgt cttgggcggc ggttatgtcg cccttgagtt tggcttccat aagccggtcg
     4921 cgatggagat ttttaagcac gtcgtcgata gtgccgctgg ctaacagccg gccacgatgc
     4981 agaattccca cgagattgca gattgccgcc aactccggca gaatgtgtga acttacgagt
     5041 atggtcttgc cgttgtctgc caacttgcgt ataagttcac gcatctgcac tctggcccgc
     5101 gggtcaaggc cgttcgccgg ctcgtccagc agaatcactt ttgggtcgtg catgagcgta
     5161 cgtgcaatgc cgacccgctg cttcatgcct ttggaaagcg tgtcgacgaa tctgtctcgc
     5221 atgtattccc cctctgtcat ttcaagcacg taatccaatc gcctgatgcg ctccttgcgg
     5281 gggattttat atgctgcgcc gaaaaagtcc agatactccc atactcgcat ctggtcgtat
     5341 acgccgaaat tgtcaggcat atagcccagc agcgatttga tttggtttct gtctttgacg
     5401 acgtcataac cgcatatgct ggcacggccg tccgacggca gaagaaggcc ggctaacatc
     5461 cggatagtcg tggttttgcc cgcgccgtta tggccgatgt atccaaatac gctgccggct
     5521 ggaatttcaa ggtccagacc ctccaatgcc acaaagccgt catacgcttt ccgcaatttc
     5581 tcacagacca gaacgctttc tgatgacatc actcaaccgt tcctttcaga gtgggggtaa
     5641 tgctgatgat tttccacatt gcctgattcg acgagccgga aatgtttatt gccgatatcg
     5701 tttgggcgat atcagacttc agcctggata gttcgataca tattgccatt ttgccttcgt
     5761 gtacggcaag tttgtccgca tctggtatcg atatgttata aacgccggac ggattgtcca
     5821 cgctcagata aacctgcaag gcgactgtgt tgaaatgacg ccgcggcgta tcggagtggc
     5881 cgggcgtcag actgatgcga gagactttca tgacgtatcc cgacgcctgc agatttatcg
     5941 ccagttcgac ggaggctttt tttagttttt tgatattttc cggcagtccc agcagataga
     6001 cgaatttgct tgggcgaatg caggaattga actttctggc cgtggtgttc cacaccggca
     6061 tttccagatt catcacgtcg ctcggcagga gagccgccgg tatagtcact tccgtgccgg
     6121 attttaccgg cgaaaacgtt acgggaatgt caattacgct tacgccctga cgctttacat
     6181 tcgcagtttg agccagcggt gaaatcgcgc ttccgtccag gtagcccagc agccgcggag
     6241 cggttattgt cctgtgaaat ccgccgtggt cgagcatcag tgatatcagt tcgttccatt
     6301 tggtgtcctg cgtcatgagc gaactggtaa attcgccttc ggcgagtttg tcggcggtgt
     6361 tcatgcggat gatattttca cctggtgcga tattgccgat tttcattaca agcatgttcg
     6421 aaaaaagcac cgcattgttt acaggctgtt tgagattgtt ttttattttt attgtagcgc
     6481 cggcagagtc gaacgacagc gatgcgctca tggggtcata cgcggcgacg gtttccgtga
     6541 aaaaacttgt ggtggcgcac gatttcattc ccttttcagg catgaacatc tgcgagtcgc
     6601 cggttacgat agtctgtgtg gtcatgttgg ccagtctgtt ttcgccggct ggcatgatta
     6661 tgccgttcat gtatttactg ccgaaatcca cgtctgaggc atcggggccg gaataaaagg
     6721 cgtaaaattc ctgtatgtac gattggccgt cgccgagtgc cgcgacgtaa cccacattgc
     6781 tcagccgggc tgtatcgctg gtgtgtcggc tgatttgaaa tattacgact gacattacgg
     6841 cgacggctgg tatcagaaat atccagagca gttcgccttt tctcaatatt ctgagcaggc
     6901 cgcccagtgc gagagtcgca aaaacgaaac cgagcagtat gccgcacggg ataaagctgc
     6961 ttggggcttg acggccggcg atttcctgga gcgtcgcaaa acctggaatt acgttcctgg
     7021 ttttatctac atatctgggg ctggcggggc gatggcggct gaattttttc gacgtggctt
     7081 tatttttgga gacgggttct gcctgcttat cgacttttac cgtctccttg accgaaacga
     7141 cgttggaaaa atttgcaggg tcgagggcct gtcgcgagtg tacctgtacg gcgacgtttt
     7201 tccagacgtt gaagttaccc tgcggctgga gtgcttgcag cgagcatgct gcaaccatca
     7261 ctacgcccat gcctgtacgt tttgccgtta acagcggcag ggtgtcgcat gtttcgagaa
     7321 cggtggcgcc gtcggctgcg agttcggcaa aatatgtcgg cttggccagc gtatatgttt
     7381 ggccgtcgac gttcaatctg tcggtgtggt gaaatcccaa cgcatggacg ccggctaacc
     7441 ggcccagccg gccttgcaac tgctcaaacg acccctcgcc gcagaatatc agcagtctgc
     7501 cgcctttacg cacccattgt tccattgccg taaattgcga gggcagtaac ttggaatcgt
     7561 cgaccggccc gaccatcagc atgtccaaca attcgtatcc gtaccagttt gtcggcagat
     7621 tttccacgtt gccggctgat atgaacaaaa tgttgcccag cggatttgaa ccgtcgtcct
     7681 tcatgtatgc aaaagaatcg ccgttgtcac cgttcactac agctgtgtgc aatgttttgt
     7741 ccggcacgcc gagtaaaatg ctttcctgaa gtgataactg ccgttcattt ccagtttcct
     7801 ggataatgaa cgtctggccg aactttgaca tgcctgattt tgtctggcca tgcctgctgc
     7861 ctgcggctat ccgggcaaaa ctgccgaacc gcattccgac ggtcacctga cggatggaat
     7921 tcgccggcga tgtaaaatca cgtcggaatt caagtctgcc gagcttgtca tccgtggctg
     7981 ttgttttgac gcttagagat aaattacggc tggcgtcgtt ttgtacttca cagattttct
     8041 gggtccaggt ccgttcgctt gagaatggat gaaacggccc gcgtatggat ttgagagtta
     8101 cggattcagt ctggcataac gccgtggatg caacggctag aaaaaccgcc agacataaaa
     8161 attttgccaa taccgccgat aaacggaatc tgtttgctgc catggatgag tttccatatt
     8221 caaaacatat tatttacgga ttattgaacc gcaaaagata ccagaaaaga atgtgttttt
     8281 gaaggtattt ttgtatattt tatatggaac ggtttttgaa acctcaggga tttactgccg
     8341 aggcattaca cattcagatg aacttccctg cccgcacaaa gtgcgatagc cagcgcgtcg
     8401 gcgacgtcag gcggttctgg gggattttct agtgacagca gagactgaat cgcaagctgc
     8461 atctgctgct tggaggcgtg gccgttgccg gtgagacttt ttttaacgcg cgtggctgat
     8521 acgctgtgca cgagtatgcc ggcgtgagct gcggcgagca tgatgacgcc cctggcgtgg
     8581 cccatgagta ttgacgtccg cgggtgggcg taatgcgaat ataactgctc gatggctacg
     8641 acgttaggcc gaatttcggc gatgatttcc tgtaaatcgg taaatatctg gtcgatacgg
     8701 cgaggcatgt cagcctttga gtctgtgcgg attgtgccgg cttccaatac cacaggctcg
     8761 gcggatgaaa aaaactccac cgccgcataa cccgtaatat tcaaccccgg gtcgatgccc
     8821 aataccctgt atgcgtccgt attgaattgc tcttcagcca ttaatcgcaa tcataacagc
     8881 tctaatcaat aaattcaaat ccgaacgcac tggtttgtca tagccgcctt gcggacagct
     8941 atgacaaacc atagcaatga atggaaaatc attctgcttt gtcatcttcg cagatggttg
     9001 cggatttttc ttcggcttcc gaaatttccg atgcaggggt cggctataat gaaaaagcct
     9061 gaaaatacga ttaagacgta tctttttaga ggacgaaaga ggcttaaaac tcttttgtcg
     9121 gattatattg gaggctgcaa aaatgaagcg gcagcagttt tttgaaattg acgggttcga
     9181 tacggctctt gccgacagtc tcaaagcctc gcatgagccc ttgcgccctg actttaccaa
     9241 caaggtcatg caacgcagga ataaaattat cgaggcggaa tttttagcca aaagtattcg
     9301 acaggaacgg cttgccctga ttgcgtgcat a
//
LOCUS       DAONFI010000051         9246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8559_length_9246_cov_4.459145, whole genome shotgun sequence.
ACCESSION   DAONFI010000051 DAONFI010000000
VERSION     DAONFI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_9246_cov_4.459145"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..815)
                     /locus_tag="PLK08_02680"
     CDS             complement(21..815)
                     /locus_tag="PLK08_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52434.1"
                     /translation="MFISANIRYTGKTKSFTREPGFRYWSVGSIVEGDRLLHYRRKTE
                     YIMQPGCLVLIPPCTPYTNTGYSNQREFWSFFSPIKTWDKLLEPYRSRNILSLVKYQG
                     TPNEREMRDCMSSIIEIQGRNYTDKNTWLNNLMERMLLLARCNDRKILEYRMDDRIEK
                     TIEFIAKNFCGYCSVKKLASIACLSPSRYAHLFKECTGIGPAAYIESVKLDKAKSLLI
                     STSLSVSEIAGLTGFCNPFYFSQRFHEKFGCSPTAYRKNSYNTNSD"
     gene            991..2421
                     /locus_tag="PLK08_02685"
     CDS             991..2421
                     /locus_tag="PLK08_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52435.1"
                     /translation="MKNIFKIGWSEVDITPCGEVELFGQYYRRLSRGVHSRIGATAMA
                     IDGGNGQCTLMISIDVVNFSGKFFDEFRENLKNKLPELDTSKLIMNAIHTHSAPQVDS
                     GKCWWEKSDSRMLSYHDFVIRQLCEAAAIAWNTRQPAGIASTFDFATVGHCRRAVYSN
                     KTAEMYGDTSRADFIGMEGGEDSTIELLFAFDGNSKPTGVIVNVACPSQIMEATYLIS
                     SDYMGKVRQLLKEKFGPDFHVLCQISAAGCQSPRDMSRTRDDEFWGERGVDVIGRRVF
                     DAVMRGYQKIAGQINFKPELVHNVKELELPRRRASYVDYTMAAEDVERLEKIQPEQEA
                     FVDFCADVKVNEEKAGRPGPYDSKLHHFVLIKNAQAVMDRYNEQHITPDYKMELHTVK
                     LGDVVFVTNPFELFLDYGLQMKARSKARQTFVVQLACGSGAYLPTERAEQLGGYGGLI
                     INGEVGSDGGKMLVDYTVKSINDLFS"
     gene            2439..3902
                     /locus_tag="PLK08_02690"
     CDS             2439..3902
                     /locus_tag="PLK08_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPS52436.1"
                     /translation="MKYILSLDVGTTSVKTVLFDVTGHEVARHVCEYELLKPKPDFVE
                     VDPDVYWHAACEGIRGIFHDSAVSKDDIVSVGVTSQGETLIVLDENGRPLCNAIVWLD
                     NRAKAEAALIAEKFSRSEVYRITGQQEIAPAWTASKILWLRNHEPEIFKKVSKILLVE
                     DYLIYRLTGEFATDHALNPSTLYYDMINGCWWREMLEFLKISEKQLPSLLNSGEIAGR
                     VIADVGLNKNTPVTVAPIDQISAAVGAGNIAAGMITETTGCALAVCATTDKPVYDEKM
                     RFGIYRHAVPGLYVMLPWIPAAGMILRWFRDEFGGGMDYSELCDIAINVPAGAEGLLL
                     LPHFCGMTLPQVNPDAKGVFYGLTLSHKKRHFVRAIMESVAFSLRESIELLEQNGLKA
                     EKIISMGGAARSRLWLQIKSDVLHRDFVTMACEESTCLGAAVLSAVGTGCYEKLNDAV
                     NAMVHPFEVVMPENGQIYDSVFYKYKKLNGLFLNSAF"
     gene            3933..5291
                     /locus_tag="PLK08_02695"
     CDS             3933..5291
                     /locus_tag="PLK08_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52437.1"
                     /translation="MRKIKAAFVGFGEVNTPREFIDNRCAAAADSLEEQGLELIKAPS
                     VMDDLDGRTADAAYKSLMAKEFDVLIVCIAGWIPSWAVFKVIEPFRYKPMILWGLSGW
                     RDGESFVTTADQAGTTALRKPMTDMGYNFKYVVNYREEKPDLSKILNYAIAARAVSLL
                     KETKLGMAGYRDMKLYGTMYDGIAFKKKLGVEIEHFELLELRQLMEKTSDSEIIKIAD
                     EVRTRWKFLKNPQPGTVENSVKLYLAIKRKIEERDFSGFSYNDVDGIKKLMGFAPAGA
                     MTLLHETMDISSVPENDAPGMVTQMMVRFLTGQCGAYLEFYEFMKTGALMGVPDYVPS
                     EIVDGPITVMPNAFGNFGEGLLNVSKLKTGKITLARFAEANGDFQMHVATADAKTPIK
                     WEEAGWAPPAPQLPSLDMIFDGSADDFVQNVMSQHYIISYGDNMAVIKDFCRILGIKI
                     LS"
     gene            5388..7859
                     /locus_tag="PLK08_02700"
     CDS             5388..7859
                     /locus_tag="PLK08_02700"
                     /inference="COORDINATES: protein motif:HMM:NF037003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52438.1"
                     /translation="MNSVKNANTECFSLLEWLVESGMISTHAKRCGALSTKNLSGSVI
                     INDEELFLKDAEVISHKTQADENGNQTYIIEFSFNDYGVVWRWEIKGNADWVELTALL
                     RNDSPDDIYVSDWKILNAQDEKGGIIDLGKNPEQVRFFRWQPWDMRVERFAATEADHN
                     SDNVCHIYDPSSGLTFFAGFVTLGRMQGYHTLHYVPTAGITQYSAGCLFGRHKIGASC
                     ELRSETLRISYHENPYAALEGWANRIYDAYKPSFECALGVCADGSAWIDAFTNEKSWP
                     EILLDFATAVREKFKGFDIGCLPAGTHQIIKDGLPGNWLTFENASGCEGGYKSLFAKL
                     WKMGFSFKLWFSPFWFLGEAQYDNILEENKENLLRDINGNLITTKVSWEFDRNHNNLN
                     DEPYLTMYYLDGTHPRTKKYLTEIFTAYRAMGARAYMLDFLSVPANARMYDDTLLPME
                     AGRKILKTIREIVGHDTHLQTAVASNPGFVGCVDAARVGRDYGEGRPMYPYHNWRNST
                     YCMHDLHFANEHLFIQNAAASWFMNRKLFINDLNCLTIDKPIPLEHARISTTMFGLSG
                     DSPMVLGDDIRNIDSERLGMIKMCMPRTQGIPVPVDLFSNVAPESYCHVLKKAIRTPW
                     DSYLLVAVFNTDNEVYNTTLDFVKLGLSPDEIFSVFEFWNGEYVGTYEKSYSCAVPAH
                     SCRLYRISRHRKYPWLLSTDMHIEQGNAEVESLEWNEKAMTLKGVATRPAGECGSLFF
                     LTPRQFRLINHDKTNTMKEVIDMQTVIRLPMEFRSDKEKFELRFEIMNTPFVARKGWL
                     PYATEEEWRKYVEAHHKPDDTRVIE"
     gene            complement(7944..>9246)
                     /locus_tag="PLK08_02705"
     CDS             complement(7944..>9246)
                     /locus_tag="PLK08_02705"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPS52439.1"
                     /translation="PALHSVGKPLADGESDHGIWPCAPLDKKYISHFPETREIWSYGS
                     GYGGNALLGKKCLALRIASVQAHDEGWMAEHMLILKLTSPEGKVKYVCGAFPSACGKT
                     NLAMLIPTIPGWKVETIGDDIAWMKFGKDGRLYAINPEAGFFGVAPGTSTKSNPNAMS
                     SVEKDTIFTNVALTEDKDVWWEGIGYPAPGKLIDWKGNEWQADKAEAPAAHPNARFTA
                     KASNCPAIAPEWEDPAGVPIDAILFGGRRPSTIPLVYQSFDWNHGVFVGSTIGSEVTA
                     AALDLKAGTVRRDPFAMLPFCGYNMGDYFAHWIKIGKTKDAKLPKLFCVNWFRKTKEG
                     KWLWPGFGDNSRVLKWIFERCDGTGKAQETPIGYMPTVDAIDRKGLDDVTDADMAELL
                     SLDKEGWLKELDMVKEHYKKFGDKLPAELSSQLQALEARLK"
BASE COUNT         2457 a         1838 c         2366 g         2585 t
ORIGIN      
        1 atcgttgagc ataatcatga tcaatccgag ttggtgttgt aactgttttt tctgtaagca
       61 gtcgggctgc atccgaattt ttcatgaaaa cgctgtgaaa aataaaaagg attgcaaaat
      121 cctgtcaggc cggctatttc agagacagac agggaagtac tgatgagaag tgattttgct
      181 ttatcgagtt tgacggattc aatatacgct gccggcccga ttcctgtgca ttctttgaac
      241 aaatgtgcgt atcttgatgg cgaaagacat gcgatggaag caagtttttt tacggaacaa
      301 taaccgcaga aatttttggc gatgaattct atagtttttt cgattctatc gtccatcctg
      361 tattccaata tttttctgtc gttgcatctg gcaagcaaaa gcattcgttc catcaagttg
      421 ttgagccatg tatttttatc ggtatagttt cggccttgaa tctctataat cgacgacatg
      481 cagtctctca tctcgcgttc attgggagtg ccctgatatt ttaccagtga cagtatattt
      541 ctgcttcgat aaggctccag cagtttatcc catgttttta tcggtgagaa aaacgaccag
      601 aactcacgct ggttggaata tcctgtgttg gtataaggcg tacatggagg tatgagcacg
      661 aggcagcctg gttgcatgat atattcggtt tttctgcgat aatgcagcaa tctgtcgcct
      721 tctacaattg agccgacaga ccagtatctg aaccctggtt cacgggtgaa actctttgtc
      781 tttccggtat accttatatt tgccgagata aacattttag cagaatccca tatgctatta
      841 gcagtttagc ggattccaat ataaatttca atcttttata attacaaatc gaaattaggc
      901 gattatggat aagcatccga aaaattttta taagatttta ggataggttt tagagatatt
      961 tttaacgttt tggcagataa agagactgat atgaaaaata tatttaaaat cggctggtca
     1021 gaagtggata tcacaccttg tggtgaagtg gaacttttcg gacagtatta tcgacgatta
     1081 tccagaggcg ttcattcacg tataggtgcg actgctatgg caattgacgg cgggaatggt
     1141 caatgtactc ttatgatatc cattgatgta gtgaattttt cagggaaatt ttttgatgaa
     1201 ttcagggaaa acctcaaaaa taagctgccg gaattggata cttcgaagtt aataatgaat
     1261 gccattcata cacatagtgc tccgcaggtg gatagtggaa aatgctggtg ggaaaagtca
     1321 gacagcagga tgctgtcgta tcatgatttt gtaatcaggc aactatgtga ggctgctgct
     1381 attgcgtgga atacaagaca accggcaggt attgcaagta cgtttgattt tgcaacggtt
     1441 ggtcattgcc gtcgtgccgt atattcaaat aaaacggcgg aaatgtatgg cgatacgtcg
     1501 agagctgatt ttatcggcat ggaaggtggc gaagattcca cgattgagct gctttttgcc
     1561 tttgatggga attccaaacc tacaggcgta atagtgaatg tagcctgtcc gtctcaaata
     1621 atggaagcta catatttgat ttcttccgat tatatgggga aagtacggca actcctgaaa
     1681 gagaaattcg gtcctgattt tcacgtattg tgccagataa gtgccgccgg ctgtcagtca
     1741 ccgcgggata tgtcaagaac gcgcgacgac gaattctggg gtgaacgtgg cgttgacgtt
     1801 atcggccggc gagtgtttga tgcagtaatg agaggctatc aaaaaattgc ggggcagatt
     1861 aattttaagc ctgagttggt acataatgta aaagaattgg agcttccgag acgcagggcg
     1921 tcttatgttg actatacgat ggctgcagag gatgttgaac gacttgagaa aatacagccg
     1981 gaacaagaag cattcgtgga tttttgtgcg gacgtaaagg ttaatgagga aaaagcggga
     2041 cggccagggc catacgacag taagttgcat cattttgtgc tgattaagaa tgcgcaggct
     2101 gtcatggata gatacaatga acaacatatc actcctgatt ataaaatgga actgcatacg
     2161 gtcaaactcg gcgacgtggt tttcgttaca aatccatttg agttatttct tgattatgga
     2221 cttcaaatga aggccagaag caaggcacga caaacttttg tagtgcaact tgcttgcggc
     2281 agcggagcgt atctgccgac tgagcgtgcc gaacagcttg gcggctatgg cgggttgata
     2341 atcaacggtg aagtcggttc tgatggcggc aagatgttgg tagattatac cgttaaatca
     2401 ataaacgatt tattctcata atatttatga tgaccgaaat gaaatatata ctgtcgctcg
     2461 atgtcggtac aacatcggta aaaactgttt tattcgatgt cactggccat gaagtagccc
     2521 gacatgtatg cgagtatgaa cttctaaagc caaagccgga ttttgttgaa gttgaccctg
     2581 atgtttattg gcatgcggcg tgtgaaggca ttcgtggaat atttcatgat tctgcggtct
     2641 caaaggatga catagtatcc gtcggcgtta ccagccaggg cgaaacgctg atagtgctgg
     2701 atgaaaatgg ccggccatta tgcaatgcga tagtatggct ggataatcgt gcaaaagcgg
     2761 aagctgcgtt gattgccgaa aaattcagtc gttccgaagt ttatcgcatt acaggccagc
     2821 aggaaattgc accggcgtgg acggcgtcta aaattttatg gctgcgcaat catgaacccg
     2881 aaattttcaa aaaagtttca aaaattttac tggtcgaaga ttatttgata tatcgtctta
     2941 cgggagaatt tgcgaccgac cacgcattga atccgtctac gctttattat gacatgatta
     3001 acggctgctg gtggcgggag atgctggagt ttttaaaaat ttcagaaaaa cagttaccgt
     3061 cgcttttgaa ttcaggcgaa atagctggca gggttattgc cgacgtcggt ttgaataaaa
     3121 acacgcctgt aacagtggct ccgatagatc agatatctgc ggctgtcggg gctggcaata
     3181 ttgcggctgg tatgataacg gaaacgaccg gttgtgcgct ggcggtctgt gctaccacgg
     3241 ataagcctgt ttatgatgaa aagatgcgat tcggcattta tcgccacgct gttccagggc
     3301 tttacgtaat gttaccgtgg ataccggcgg ctggcatgat tttacgatgg tttcgcgatg
     3361 aatttggcgg cggtatggat tattcagagt tgtgcgatat cgccataaat gttccagccg
     3421 gggctgaggg tttactgctg ttgccgcatt tttgcggaat gacgttgccg caagtaaatc
     3481 cagatgctaa gggtgttttt tatggattga cgctgagcca taaaaaaagg cattttgttc
     3541 gtgcaatcat ggagtcagtt gcattttcac tcagagagag cattgaactg cttgagcaga
     3601 atggcctcaa agcggagaag ataatttcta tgggtggcgc agcaaggagc agattatggc
     3661 tgcagataaa gtcggatgtt ttgcataggg attttgtgac aatggcatgc gaagaatcga
     3721 cttgcctcgg cgcagccgta ttatctgccg tcggcactgg ctgttatgaa aagttgaacg
     3781 atgcagttaa tgccatggtt catccatttg aagtggttat gcctgaaaat ggtcaaattt
     3841 atgattctgt attttataaa tataaaaaat tgaacggttt gtttttaaat tcggcatttt
     3901 gaataaaata ttttttataa cagaggtaac caatgcgtaa aataaaagcc gcctttgtcg
     3961 gattcggcga agttaatacg ccgcgtgaat tcatagataa tcgttgtgct gctgcggcag
     4021 atagccttga agaacagggt ctggaactta taaaagctcc atctgttatg gacgaccttg
     4081 acggcagaac ggcagacgcg gcatataaga gtttgatggc aaaagaattc gatgttctga
     4141 ttgtttgcat tgccggttgg ataccgtcat gggccgtttt taaggttatt gagccgttca
     4201 ggtacaagcc tatgatatta tggggactga gcgggtggcg ggatggtgag agctttgtga
     4261 cgaccgccga tcaggctggt actaccgcgc tgcgcaaacc gatgactgat atgggatata
     4321 atttcaagta cgtcgtaaat taccgtgaag aaaaacctga tttatcaaaa atattaaatt
     4381 atgcaatagc cgccagagcc gtcagtttat tgaaagaaac caagcttgga atggccggtt
     4441 accgcgatat gaaactttac ggaacaatgt atgacggcat agcgttcaaa aagaaacttg
     4501 gcgtggaaat cgaacatttc gaattgttgg aattgcggca gttgatggaa aagacgagtg
     4561 attcggaaat aataaaaatc gccgacgagg ttcgtactcg ctggaaattt ttgaaaaatc
     4621 cgcagcctgg cacggttgag aattccgtaa aactttatct ggcaatcaaa cgtaaaattg
     4681 aagaacggga tttttctggt ttttcatata atgacgttga cggcattaaa aagttgatgg
     4741 gttttgcgcc tgcgggggcc atgacgctgc ttcacgaaac aatggatata tcgtctgttc
     4801 cggaaaatga cgcgccaggc atggtgacac agatgatggt cagattttta accggccagt
     4861 gtggagcata cctggaattt tacgaattta tgaaaaccgg tgcacttatg ggcgtaccgg
     4921 attatgtccc ttccgagata gttgacggcc cgattacggt tatgccgaac gcttttggaa
     4981 attttggcga aggtctttta aatgtgtcca aactgaaaac tggaaaaatt acattagcaa
     5041 gatttgcgga ggcaaatggc gattttcaga tgcacgtcgc gacggctgat gcaaaaacgc
     5101 cgataaaatg ggaagaagcc ggctgggctc ctccggcccc acagcttccg agtctggata
     5161 tgatattcga cggctctgct gacgattttg tccaaaatgt catgagtcag cattatataa
     5221 tttcatatgg cgacaacatg gcggtaatta aggatttttg ccgtatatta ggaattaaga
     5281 ttctttcgta ggaaacaggt ttaaactttt taccatgtct gacaatgtga atgtatcaag
     5341 ttgaattaag tgcgtttttt gggaaaaaat ttaaaaggaa ttttaagatg aattctgtga
     5401 aaaatgcgaa tacggaatgt ttctcacttc tggagtggct ggtggaaagt ggaatgatta
     5461 gcacgcatgc aaaacgatgt ggtgcactgt cgacaaaaaa tctttcaggt tcggttatca
     5521 taaatgacga agagcttttt ctgaaagatg ccgaagttat ttcgcataaa acgcaagccg
     5581 atgagaatgg taatcagaca tatattattg aattttcatt caatgattac ggagttgttt
     5641 ggcgttggga aattaaaggc aatgctgatt gggttgagct gacggcgctg ctgcgaaatg
     5701 acagtcctga tgatatttat gtaagtgact ggaaaatttt aaatgcccag gatgaaaagg
     5761 gagggattat agaccttggc aagaatccgg agcaggttcg ctttttccga tggcagccgt
     5821 gggatatgcg ggttgagcgt tttgccgcta cggaagccga ccacaactca gataatgttt
     5881 gtcacattta tgatccttca tccgggctca ctttttttgc aggctttgtg acgctcggcc
     5941 gcatgcaggg ttatcatacc ttgcattatg ttccgactgc tggaattaca cagtatagtg
     6001 ccggctgtct gtttggcagg cacaaaatcg gggccagctg tgaattgcgg tctgagacgc
     6061 ttcgtatcag ttatcatgaa aatccgtatg ctgcgcttga aggttgggct aaccgcatat
     6121 atgacgcata taaaccgtca tttgaatgtg ctcttggcgt ttgcgcagac ggttctgcat
     6181 ggatagatgc ctttacaaac gaaaaatcat ggcctgaaat attgcttgac tttgccactg
     6241 ctgttagaga aaaattcaaa gggttcgata ttggttgcct gccggctggc acgcatcaaa
     6301 taatcaaaga cggtttaccg ggtaattggc tgacatttga aaatgcgtct ggctgtgaag
     6361 gcggatataa gtcgctgttc gcaaaattat ggaaaatggg attcagcttc aaattatggt
     6421 tttcaccgtt ctggtttctg ggggaggccc aatacgataa catattggaa gaaaataaag
     6481 aaaacctgct gagagatata aacggcaatc tgataaccac caaagtaagc tgggaatttg
     6541 accgtaatca taataatctt aacgatgaac cctatctgac catgtattat cttgacggca
     6601 ctcatcccag gactaaaaag tatctgacag aaatattcac ggcataccgg gctatgggtg
     6661 cgcgtgcata tatgcttgat tttctgtcag tgccggctaa cgccaggatg tatgacgaca
     6721 cgttgcttcc gatggaggct ggccgtaaaa tacttaaaac catacgtgaa atagtcgggc
     6781 acgatacgca tcttcagacc gcagtagctt ctaatcctgg ttttgtcggc tgtgttgatg
     6841 cggcgcgtgt aggtcgtgat tacggcgaag gccggcccat gtatccttac cataactggc
     6901 ggaattccac ttattgcatg catgatctgc attttgcaaa tgaacattta tttattcaga
     6961 acgcggcggc ttcgtggttc atgaatcgta aactttttat aaacgacctt aattgcctga
     7021 ccattgacaa gcctataccg cttgaacatg ccagaatatc aaccaccatg ttcggcctta
     7081 gcggtgacag cccgatggta cttggggatg atatacgaaa tatcgattcc gaacgtcttg
     7141 gcatgattaa aatgtgtatg ccgagaacgc agggcatacc tgttcctgtt gatttattct
     7201 cgaatgttgc gcctgaaagt tactgccatg tgctgaaaaa ggccatacgc actccctggg
     7261 attcatacct gcttgtcgcg gtgttcaata ccgataatga agtatataac actacgcttg
     7321 attttgtgaa attggggctt tcccctgatg aaattttcag cgtttttgaa ttctggaacg
     7381 gtgaatatgt cggcacatac gaaaaatctt attcctgtgc agttcctgcg cattcatgca
     7441 gattatatcg catatcgcgc catcgcaaat atccatggct tttgtcgacg gatatgcata
     7501 tagagcaggg caatgcggag gtggagtctc tggaatggaa tgagaaggcg atgacactga
     7561 aaggtgtggc gacgcggcct gccggcgaat gcggcagtct gtttttcctg accccgcgcc
     7621 agttcaggct gattaatcat gataagacga ataccatgaa ggaagtcatc gacatgcaga
     7681 ccgtaatccg tctgccgatg gaattccgct cagacaagga aaaatttgag cttcgctttg
     7741 aaatcatgaa tacgccgttt gttgcgagaa aaggttggtt gccctatgcc actgaagaag
     7801 aatggcgtaa atatgtcgag gcacaccaca aacccgacga taccagggta attgaataat
     7861 gaattctgtt cggttcggat aaaaaaaaga gccggcgctt taatttttag tcaaagcgtc
     7921 ggctcttctt tttatatttt aaactatttc agtctggctt cgagagcctg aagctggctg
     7981 gagagctcag ccggcaattt gtcgccgaac tttttataat gttccttgac catatcaagt
     8041 tccttcagcc agccttcttt atcaagagac agcaattcgg ccatgtcagc atcggtaacg
     8101 tcgtcgaggc ctttgcggtc gattgcatcg acggttggca tatatccgat gggtgtttcc
     8161 tgggctttgc ctgtgccgtc acaacgttcg aatatccact tgaggactcg gctgttgtcg
     8221 ccgaagccgg gccacagcca cttgccttct ttggttttgc ggaaccagtt cacgcagaat
     8281 agtttgggca gctttgcgtc ttttgtcttg ccgattttta tccagtgtgc gaaataatcg
     8341 cccatattgt aaccgcagaa cggcagcatg gcgaatgggt cgcgacggac tgtgccggcc
     8401 ttgaggtcaa gtgctgctgc cgtgacttcc gagccgatgg tcgaacccac gaatacgccg
     8461 tgattccagt cgaacgactg ataaaccagc ggaatcgtgc tgggccgacg gccaccgaat
     8521 aggattgcat ctatcggtac gccggccggg tcttcccatt ccggagcaat cgccggacaa
     8581 tttgaagcct ttgcagtgaa acgggcattg ggatgggcgg caggcgcttc agccttgtcg
     8641 gcttgccatt cgttgccctt ccaatcgata agtttgcctg gtgcaggata gcctatgcct
     8701 tcccaccaga catctttgtc ctcggtgagt gcgacgttgg tgaagatggt gtccttttca
     8761 actgaggaca tggcgttggg gttggacttt gtggaagttc caggcgccac gccgaaaaag
     8821 ccggcttccg gattgattgc atacagacgg ccgtccttgc cgaacttcat ccacgcgata
     8881 tcgtcgccta ttgtctcgac tttccagccg ggaatggtcg ggatgagcat cgccaggttc
     8941 gttttgccgc aggcgcttgg aaatgcgccg caaacgtatt tgaccttgcc ttcggggctg
     9001 gtaagtttca ggataagcat gtgttccgcc atccagcctt cgtcgtgtgc ctggacggat
     9061 gcgatacgca acgccagaca ttttttgccc agcagtgcgt tgccgccgta acctgaaccg
     9121 taagaccaga tttcacgggt ttcgggaaag tgcgagatat acttcttgtc cagcggcgcg
     9181 cacggccata tgccgtggtc ggattcgccg tcagccagcg gtttgcccac tgaatgcagg
     9241 gctgga
//
LOCUS       DAONFI010000052         9149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8684_length_9149_cov_4.333956, whole genome shotgun sequence.
ACCESSION   DAONFI010000052 DAONFI010000000
VERSION     DAONFI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8684_length_9149_cov_4.333956"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1580
                     /locus_tag="PLK08_02710"
     CDS             129..1580
                     /locus_tag="PLK08_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPS52440.1"
                     /translation="MIPYDAKKFIAVVTMLAALGGSGVFAQTSQPSTQEALRKEIIEM
                     SRNDRLSNLFTKIYQAVSPSVVVIHTARVSSADILSYYQDRYIREQFNHDYNVGLGSG
                     VIIDAENGYVVTNNHVVANVQRVEVILSDGRILRAQWVRSDPLSDIAVIKIPPQNLTA
                     TPLGDSDRLEVGSLVLAFGAPLGLPQTVTAGIVSAKGRKTTGMDYQDFIQTDAAINLG
                     NSGGPLVNMRGEVVGINTLILSSTGMYAGVGLAIPSNMVRNIMKQLVQKGLVMRGYLG
                     AYIQNVSPALARNMNLPHNNGALITNVFTDSPAGKAGLLERDFVISVNNKTIRNANDL
                     RNATASLMPEETATMKILRDGKEIELKVIMGRSRRKTIPPAKTFLNRPAETFEDQVGL
                     FCKTLTPTTAIQYGYAAEMNGAVVAKVLAGSEAFEANIVPGMLVTHVNDNPIKSESEL
                     KTAIDRSRGTGIRLTVTTPIGGKWYIFLPLEIF"
     gene            1772..2302
                     /locus_tag="PLK08_02715"
     CDS             1772..2302
                     /locus_tag="PLK08_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPS52441.1"
                     /translation="MQNGKMMKQVLWLNILPAIIGLLLTAGCASKPKQNVQMDTSELN
                     PPAESTAANAPAVADEPVAPATAAPVTTEAAYKPAPSPAPLNPDAEKPKPSPRPIRKV
                     EPATLTEAMNTPKKPAASSKVNYYIVRHDDTFWSISRRVYGSGKYWKTIQDANPDVSP
                     ERLMVGQKIVIPEIAK"
     gene            2423..3205
                     /locus_tag="PLK08_02720"
     CDS             2423..3205
                     /locus_tag="PLK08_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52442.1"
                     /translation="MVKVWNALPYVVLACILAIICYCTCGGGEKNMGLKITSEDGFIE
                     WGTCVAFGITGLLGMCAAVMKRKMLTKNQLIFLVVFSIICFLAVGEELSWGERIFGFA
                     PPENMETTSSSAVTFGHNDVTMHNLDFKFGKVSFSIGGMLFGVTLIVALAIHGIVLPI
                     LYKKRHPKIVKFVDKLGLFLPPLNFGILVTAAALMFYILRRTTIDDMTEPNEFKEFFV
                     PVVYAAIVAVTFFNGKTKGERVCLEFLFILFMLGITASVTKM"
     gene            3221..4270
                     /gene="tsaD"
                     /locus_tag="PLK08_02725"
     CDS             3221..4270
                     /gene="tsaD"
                     /locus_tag="PLK08_02725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPS52443.1"
                     /translation="MEKRIVILGFESSCDETAVSLVVDGRKVLSDVVASQIDQHRRYG
                     GVVPELASRAHVEAVNTVLADALAQSGIKRDEISAVAVTHQPGLIGSLLVGLMAAKTT
                     AWLLGVPLIGVNHVCAHAYAAALDDEPINYPAVALVCSGGHTSLYHLQSPTKMELLGS
                     TIDDAAGEAYDKVASILHLGFPGGPVIDRAARRGNGHAVDFPRTLLKGQSLDFSFSGI
                     KTAVLYHVNGVPNAKTDNGHAKGIENFSSQEVDDIAASFQDAVTDILTIKMRRAAKIT
                     GARTLILGGGVAANSGLREKAQKVADKIGCTLRRPPMKYCTDNAAMIAGLGYHLLLAG
                     QIADLRLSATPTVRI"
     gene            4359..5705
                     /locus_tag="PLK08_02730"
     CDS             4359..5705
                     /locus_tag="PLK08_02730"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HPS52444.1"
                     /translation="MAWQSRIRVFSSKTARNDGRYVLYWMQQSQRTRYNHALEYATEE
                     ANRLGRPLMVAFCVAAYPQANVRHYAFMLQGIDEVRRELEKRHACFITRIGQPSDLIS
                     ELAKPAALLITDRGYTPIQSRWRQDVCKNCDVPVIEVESDVVVPVDIASDKREYSAAT
                     LRRKITPLIDEYLLLPRQRSLKIAADAINEKSDDISLKNVTKKLHLDASVSPVDGFCG
                     GYSQAEKRLTEFVSKKLRGYDELRNDPTKNFTSGLSAYMHFGQISPIEIALAVKTADA
                     PKPDRDAFLEQLTVRRELAMNFLRFCQKADDYESLPGWARATLESHDKDRKKTYTLRQ
                     LEQASTVDEYWNAAQRQLVRTGTIHNYMRMYWGKKILEWAPSAKLGFEWCVHLNDRYA
                     LDGRDANSYAGIAWCFGNHDRPWSERPIYGNVRCMTAGGLERKFDIRSYAKMWSEP"
     gene            complement(5865..7202)
                     /locus_tag="PLK08_02735"
     CDS             complement(5865..7202)
                     /locus_tag="PLK08_02735"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPS52445.1"
                     /translation="MRKRIVFSVIAAVLAAWIGMADAQAADEEVQATIKQLQAQLEAQ
                     QKELKQLKNQQVDVAELLEKQRANEDKLYKVADDIGNVDWLHGFKISGDLRLRYQGQS
                     YRGYNQTVDKDKSKPWRNRSRIRFRIGFEKTLLDDQFLVAARLATGDYDTKYPGNNEP
                     TSTNVTMTNDFSKKDIWLDQAYVKYTPKFLKGLEVIGGKMPTPMVHTDMIWDDDVNPE
                     GFWAQYKYSQLPGGFKPFVGAGYFIVKENSQYDYNDCTMADFELGFDWELAKDFKWTL
                     AANYYVWDNYQYSYNYGAANGNDVDSDKNLAAGAFRVFNLTNEFKFKFLGKENKAYFD
                     WAYNCADDDPNYDYKNQNKAYAVGYKFGNNKKKGDWSVGYKYAYIEANATPGYFADSD
                     FGYVNKKGHVIDAKYNLADFLTVGGKVFLTQDIAGPMAGPTDVTTLFDVEWKF"
     assembly_gap    7403..7412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7542..8000
                     /locus_tag="PLK08_02740"
     CDS             7542..8000
                     /locus_tag="PLK08_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS52446.1"
                     /translation="MVLALKDNSAPLELVVTGEVASWLPALEQVVGPNWLKTRRVRSG
                     HELLEVVRHGRADAAVLDDECDWDLDVLQMLRMLRQLDRSLQVVVVTHHTERQWMEAA
                     LKLAAYSVLTKPLGFEPLVRQIHGIMLKMHKQLNSQPTAIMKLDDWNKNW"
     gene            8252..8563
                     /gene="groES"
                     /locus_tag="PLK08_02745"
     CDS             8252..8563
                     /gene="groES"
                     /locus_tag="PLK08_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPS52447.1"
                     /translation="MAKAKCTKCKIRPLGDKVLVKRLEAESITAGGIVLPDTAKEKPK
                     QGTVLAVGEGSLNKDGSRTPLQVKTGDTVLFTSYAGTTVKIDGDELMLMDESDILAII
                     D"
     gene            8659..8949
                     /locus_tag="PLK08_02750"
     CDS             8659..8949
                     /locus_tag="PLK08_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52448.1"
                     /translation="MAKAAGIFMDVTTRWAEKLQVDMTVLEWAFDNLRHNASNEGCHN
                     FPSRDDVASNDIFFNAVRRMYEIRTGRVLEKSEYPMLRRMILKVHKQGKTPR"
     gene            8972..>9149
                     /gene="groEL"
                     /locus_tag="PLK08_02755"
     CDS             8972..>9149
                     /gene="groEL"
                     /locus_tag="PLK08_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514159.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPS52449.1"
                     /translation="MAKKKIIFDVDARSSILKGVKQLARAVKVTLGPTGRVVVLQKSF
                     GSPTVTKDGVSVATT"
BASE COUNT         2431 a         2303 c         2258 g         2147 t
ORIGIN      
        1 gaatcctcga caagcccggc atgattgata tgcttatgga gtgcacctcg ccaaaagaat
       61 ttggagaaac tctcgcccat cgtgaaatag aattgatagg cctgcacgat gaatgataaa
      121 attgtgacat gattccatac gatgccaaaa aattcattgc cgtcgtgacc atgctagccg
      181 cattaggcgg ttcaggtgtc tttgcgcaaa catcccagcc atcgactcag gaggctctgc
      241 gcaaggaaat cattgagatg agcagaaacg accggctttc aaaccttttt accaaaatat
      301 atcaagccgt ctctccctcc gtcgtcgtaa tccataccgc cagagtctcc tctgccgaca
      361 ttctttcata ttatcaggac agatacattc gcgaacagtt caatcacgac tataacgtcg
      421 gcctgggcag cggcgtgata atcgacgcag aaaacgggta cgtcgtcacg aacaatcacg
      481 tggtggctaa cgttcagcgt gtcgaagtga tactcagtga cggcaggatt ttgagggcgc
      541 agtgggtgcg cagcgacccg ctcagcgata tagccgtcat aaaaataccg ccgcaaaatc
      601 tcactgctac gccactgggc gacagtgacc ggctggaggt cggcagcctg gtgctggcgt
      661 tcggagctcc gctgggcctg ccgcagaccg tgacagcagg catagtctcc gcaaaaggac
      721 gcaaaacaac aggcatggat taccaggatt tcatccagac cgatgccgcg ataaacctgg
      781 gcaattcagg cggccctctg gtaaacatgc gaggcgaagt ggtcggcata aatacgctga
      841 tactctcatc aacaggcatg tatgccggcg tcggcctggc gattccgtcc aacatggttc
      901 ggaacataat gaaacagctc gttcaaaaag ggctggtaat gcgcggctac ctcggcgcat
      961 acattcaaaa cgtcagcccg gcgctggcca gaaacatgaa tctgccgcat aacaacggcg
     1021 cactgataac caacgtgttc accgacagcc cagccggcaa agccggcctg ctggaacgcg
     1081 atttcgtaat ctcggtcaat aacaaaacca tccgcaacgc caacgatttg cgaaacgcca
     1141 ccgcctctct gatgccggaa gaaaccgcga caatgaaaat ccttcgcgac ggcaaagaga
     1201 ttgaattaaa agtgattatg ggccgctctc gcagaaagac gattccgccg gcaaaaacat
     1261 ttttgaaccg accggctgaa acattcgaag accaggtggg tctgttctgc aaaacgctta
     1321 caccaaccac cgccatacag tacggctatg ccgcagaaat gaacggcgca gtggtggcga
     1381 aagtattagc cggcagcgaa gcgtttgaag caaacatagt acccggcatg ctcgtcacgc
     1441 acgtaaacga caatccgata aaatcggaat cggaactgaa aacggctatc gaccgttcgc
     1501 gaggtacggg aatacggctt accgtcacga cacctatcgg cggaaaatgg tatattttcc
     1561 tgccgcttga aattttttaa tatgactgta cgctatacag gtacttttaa aaacttttta
     1621 acatgaccat tctgaatata taggttactt ttaaaaattt tttagaacga ccgttcagaa
     1681 tatataggtt actttttaaa actttttaat gcgactgttc agaatatgct attttaaaaa
     1741 ttttgaaaaa ttaacgtgaa aggattttta tatgcaaaat ggaaagatga tgaaacaggt
     1801 tttgtggctg aatattctgc cggcgattat aggccttctg ctgacggctg gctgcgcaag
     1861 taagccgaaa cagaatgttc agatggacac gtccgaactg aatccaccgg cggagtcgac
     1921 agccgcgaat gctcccgccg tagccgacga accggttgcg ccggccactg ccgcacccgt
     1981 aacgacggag gctgcctata agcccgcgcc atcgccggca ccgttgaatc cagacgctga
     2041 aaaacccaaa ccttcgccca ggcctatcag aaaagttgaa ccggccacgc ttaccgaagc
     2101 aatgaatacc ccaaaaaaac cagccgcttc gtcgaaagtg aactattaca tagttcgaca
     2161 tgacgacact ttctggagca tatcacgtcg cgtgtacggc tcgggcaaat attggaaaac
     2221 cattcaggac gcaaatccgg atgtcagccc cgaacgactg atggtcggcc aaaaaatcgt
     2281 aattcccgaa atcgcaaaat aaaaatcttt accttttcgc tttgtatacg gaaatctgtt
     2341 aaactggagc agttctcaaa actcttttgc ctttgggaac tgctccagtc agtttgaaaa
     2401 actaaaaatg aaaggatttt ttatggttaa agtctggaac gctctgccgt atgtcgtact
     2461 tgcatgcatt ttggcgataa tctgctactg tacatgcggc ggcggcgaaa aaaacatggg
     2521 tctcaagata accagtgaag acggctttat cgaatggggc acttgcgtgg cgttcggcat
     2581 taccggtctg ctgggcatgt gcgcggcggt tatgaaacgg aaaatgctta caaaaaatca
     2641 attaatcttt ctggtagtgt tttcgataat atgcttttta gccgtcgggg aagaactcag
     2701 ctggggtgag cggatattcg gattcgctcc gccggaaaac atggaaacca cgtccagctc
     2761 ggcagtcacg ttcggacata acgacgttac gatgcataat ctggacttca aattcggcaa
     2821 ggtcagtttt tcaatcggcg gaatgctttt cggcgtgacg cttatcgtgg cgttagccat
     2881 ccacggcata gtgctgccga tattgtataa aaaacgtcat ccgaaaatcg taaaattcgt
     2941 cgacaaactc ggcctttttc tgccgccgct gaacttcggc atactcgtta ccgccgccgc
     3001 actgatgttt tacatcctta gaagaacaac catcgacgat atgaccgagc cgaacgaatt
     3061 taaggaattt ttcgtgcccg tcgtttatgc agccattgtc gccgtcacgt tttttaacgg
     3121 aaaaaccaaa ggcgaacggg tctgccttga atttcttttt atcctcttca tgctcggcat
     3181 aaccgcgagc gtaactaaaa tgtaattcgc tgtgcatgaa atggaaaagc gaatcgtaat
     3241 attgggtttt gaaagcagtt gcgatgaaac cgcagtttcg ctcgtagttg acggccgaaa
     3301 agttctttcg gacgtcgttg ccagccagat agaccagcac cgccgttacg gcggcgtcgt
     3361 gccggaactc gccagccgcg cacacgtcga agccgtcaat accgtcctgg ccgacgctct
     3421 cgcgcaaagc ggaataaaac gcgacgagat atccgccgtc gccgtcacgc atcagccggg
     3481 gctaatcggt tcattgctgg ttggattgat ggcggcaaaa acgacggcgt ggctgctcgg
     3541 cgtgccgctg attggagtaa atcacgtctg cgcgcacgca tacgccgccg cactggacga
     3601 cgaacccatc aactacccag ccgtcgcact cgtatgttcg ggcggccata cgtcgctgta
     3661 tcatcttcaa tcgccgacga aaatggaact gctcggcagc accatcgacg acgcagccgg
     3721 cgaagcctac gacaaggtgg catccatact tcatctgggt tttccaggcg gaccggttat
     3781 cgacagggca gcccgccgcg gcaatggcca tgccgtggat tttccacgca cgctgctcaa
     3841 aggccagtcg ctcgactttt cattcagcgg cataaagacg gctgtcctgt atcacgtcaa
     3901 cggcgtgccg aacgcaaaga ccgacaacgg tcatgcgaag ggcattgaaa acttttcatc
     3961 gcaggaagtg gacgacatcg ccgccagttt ccaggacgcc gtaaccgata tcctcaccat
     4021 caaaatgcgg cgtgccgcca aaatcaccgg cgccaggacg cttatacttg gcggcggcgt
     4081 ggcagcaaac tcaggccttc gtgaaaaagc acaaaaagta gcagataaaa tcggctgtac
     4141 gctgcggcgg ccgccgatga aatactgcac cgacaacgca gccatgattg ccgggttggg
     4201 atatcacctg ctgctcgccg gccaaatcgc cgatttacga ttatccgcca cgccgactgt
     4261 gcgcatatag agcggttttt aaaaaattta aaaagtgccc gccaaaaatt ttggggatta
     4321 tagccgttca caaaaatcct ccgactataa tgtcttgcat ggcatggcaa tcgagaattc
     4381 gcgtcttctc ctcaaaaacc gcccgcaacg acggcagata tgtgctttac tggatgcagc
     4441 agtcgcaacg aacccgttat aaccatgcgt tggaatatgc aacggaagaa gccaaccggc
     4501 ttggccggcc gctgatggtt gcattttgcg ttgctgcgta tccgcaggct aatgtccggc
     4561 attacgcgtt catgctgcaa ggcatagacg aagtgcgtcg cgaacttgaa aaacgtcatg
     4621 cctgtttcat aacgagaatc ggccaaccgt ccgacttaat aagcgaactc gccaaaccag
     4681 ccgccctgct cataaccgac agaggttaca cccccataca atctcgctgg cggcaggatg
     4741 tctgtaaaaa ttgcgacgta ccagtaattg aagtcgagtc ggacgtcgtc gttccagtcg
     4801 atatcgccag tgacaaacgg gaatattcgg cggctacgct caggcggaag ataactccgc
     4861 ttatcgacga atatcttttg ttgccgcggc agcgcagcct gaaaatcgcg gctgacgcca
     4921 tcaatgaaaa atccgacgat atttctctca aaaacgttac gaaaaaattg catcttgacg
     4981 catccgtctc ccctgtcgac ggtttttgtg gcggatattc gcaggcggaa aagcgtctga
     5041 ctgaattcgt gtcaaaaaaa ctccgcggtt acgacgaatt gcgcaatgac ccgacgaaaa
     5101 attttacgag cggcctcagc gcgtatatgc atttcggcca gatttcgccg atagaaatcg
     5161 cccttgccgt caaaaccgcc gatgcgccaa aacctgaccg cgatgccttt cttgaacagt
     5221 tgacagtccg ccgtgaactg gcgatgaatt tcctgcgatt ctgtcagaaa gcggatgatt
     5281 atgaatcatt gccaggctgg gcgcgtgcca cgcttgagtc gcacgacaaa gaccgcaaaa
     5341 aaacatacac gctccggcag ctcgaacagg cctccactgt cgatgaatat tggaacgcag
     5401 cccaacgaca gcttgtacga accggaacaa tacataacta catgcggatg tattggggca
     5461 aaaaaatcct cgagtgggcg ccgtcggcga aacttggatt tgaatggtgc gtacatctga
     5521 acgaccgtta cgcactggac ggccgcgacg ctaacagtta tgcgggaatt gcgtggtgct
     5581 tcggcaatca tgaccggccg tggagcgagc ggcctattta cggcaacgtg cgatgtatga
     5641 cggcaggcgg acttgaaaga aaattcgaca tccgcagtta tgcgaaaatg tggagcgagc
     5701 catagcaaca aatggtgtcg tacgttatct ttgaaaatag aacctatccc aagaacatgg
     5761 gcgttgccat tggtggatag gtgctattta aaaaccccgt ctcgacggtc aagacggggt
     5821 ttcgtgcaat ggcctataaa aaggctgtat tcagaagaat ttgtctaaaa tttccattcg
     5881 acgtcaaaca gggttgttac gtcggtcggg cctgccatcg ggccagcgat atcctgggta
     5941 aggaagacct tgccgccgac ggtcaggaaa tcggcaagat tgtacttggc gtcgattaca
     6001 tggcccttct tgttgacgta gccgaaatca ctgtcggcaa aataaccagg cgtggcgttg
     6061 gcttcgatat atgcgtattt atagccgacc gaccaatcgc cctttttctt gttgttgccg
     6121 aacttatatc cgaccgcata agccttgttc tgattcttgt aatcgtaatt cgggtcatcg
     6181 tcggcacagt tataggccca gtcgaaataa gccttgtttt ctttgccaag aaatttgaac
     6241 ttaaactcgt tggtcaagtt aaaaaccctg aatgcaccgg cggcaagatt cttgtccgaa
     6301 tcaacgtcat tgccgttggc ggcgccgtag ttataggaat attgataatt atcccatacg
     6361 taatagttgg ctgccagcgt ccatttgaaa tctttggcca actcccagtc aaagcccaat
     6421 tcaaagtccg ccatcgtgca gtcgttataa tcatactgcg agttttcctt gacgataaag
     6481 taaccggcgc caacgaacgg cttgaatccg ccaggcagtt gtgaatattt atattgtgcc
     6541 caaaaacctt ccggatttac gtcgtcgtcc catatcatat ccgtatgcac catgggggtg
     6601 ggcatcttgc cgccgatgac ttccagccct tttagaaatt tgggcgtata tttgacgtac
     6661 gcctgatcca gccagatgtc ttttttgctg aaatcgttag tcatcgtcac gttggttgac
     6721 gtcggttcat tatttccagg atatttggta tcataatcgc ccgtcgccag ccgtgccgcc
     6781 accaggaact ggtcatccag cagggtcttt tcaaatccta tacgaaagcg gattcgcgac
     6841 cgattgcgcc acggcttgga tttatccttg tcaaccgtct gattatatcc gcggtagctt
     6901 tggccttgat agcgcagcct taaatcaccg ctaattttaa agccgtgcag ccaatccaca
     6961 ttgccgatat cgtcggcaac cttgtagagt ttgtcttcat tcgcccgctg tttttcgaga
     7021 agctcggcaa cgtcgacctg ttgatttttg agctgtttga gttctttctg ctgggcttcg
     7081 agttgtgcct gcaactgctt tatggtagcc tgcacctctt catccgccgc ctgagcgtcg
     7141 gccatcccaa tccatgccgc caacacggcg gcaatgacgc tgaaaacgat tctttttctc
     7201 atttatctat cctttacgaa aatgaccata tccggccgac cgtcagcctg atttctcaat
     7261 gaaaagttaa agaattgaaa ttaagaaagt attaggatgt gtctaagaac ccattaaata
     7321 agaccaccgg ttgaggttgg tttttgacag acgactctcg tcagacgaca acctgccata
     7381 tcggcaaaaa ttcagctttt ttnnnnnnnn nntttttgcg catttgggca gttttcgaca
     7441 aacgacaaaa gtgctatttg ttttgtaact tctttagtgt acgcgtatta tgtgctttgg
     7501 cattatgctt gctgctataa aagaacctgc gaggtgtatt tatggtattg gcgttgaagg
     7561 acaactcggc tccgctagaa ttggtagtta ccggcgaggt tgccagttgg ctgccggcgc
     7621 tggagcaggt ggtagggccg aattggctca agacgcggcg tgtgcgcagc ggccatgaat
     7681 tgttggaggt cgtccgccac ggccgagccg acgctgccgt attggacgac gaatgcgact
     7741 gggacctgga tgtgctgcaa atgctccgga tgcttcgcca gcttgacagg tcgttgcagg
     7801 ttgtggtcgt tacacaccac acggaaaggc agtggatgga agctgcgttg aagctggcgg
     7861 catacagcgt tttgaccaag ccgctgggct ttgagccgct ggtacgacag attcacggca
     7921 taatgctcaa aatgcacaag cagcttaact cgcagcctac cgcaatcatg aaacttgatg
     7981 attggaataa aaactggtaa aaatttgcct gtccgcccga cggcggatgt tttttttcta
     8041 cgatttttgt taaaaaaacg taggattaag acggacataa tgaatctcta cgggttaaaa
     8101 cccgtagcat catagtcatc aggctttcgc ctgtccgcct cacggcggat actttcatct
     8161 acggtttaaa aaccgtagaa ttaagacgga catattaaaa aaacgcgttg aaaaggcaaa
     8221 aggaaataac atcgttttga ggagacagaa aatggctaaa gccaaatgca cgaaatgtaa
     8281 aattcgcccc ctgggtgaca aggtcctggt aaagcgtctg gaggcggaat ccatcacagc
     8341 cggcggcata gtcctgcccg ataccgccaa ggaaaaaccc aagcagggaa cggttctcgc
     8401 cgtcggcgaa ggcagcctga acaaggacgg cagccgtacc ccccttcagg tcaaaaccgg
     8461 cgatactgtc ctgtttacca gctacgccgg aactaccgtc aaaatcgacg gcgacgaact
     8521 catgctcatg gatgagagcg acatcctggc aatcattgac taaataattg ttaggacagc
     8581 tttctgactt gaggatagga ttcctctagc cgccactcaa gctgcccatt tagttatctc
     8641 ttttcaggag tattaattat ggcaaaggca gctggaattt tcatggatgt aacaacgaga
     8701 tgggcggaaa agcttcaagt cgatatgacg gttctcgaat gggcattcga caatcttaga
     8761 cataatgcat ctaacgaagg atgtcataat ttcccatcaa gagatgatgt agcctctaat
     8821 gacattttct ttaatgcagt tcgccgaatg tatgaaataa gaactggcag agtgcttgaa
     8881 aaaagtgaat atcctatgct cagaagaatg attctaaaag tacataaaca aggtaaaacc
     8941 ccaagataat tgcagctagg agattattga aatggcaaag aagaaaataa tatttgacgt
     9001 agatgcccgt tcgagcattc tcaagggcgt caaacagctg gcgagagccg tcaaggtcac
     9061 cctcggtcct accggccgtg ttgtagtact gcagaaaagt ttcggcagcc cgaccgtcac
     9121 aaaagacggc gtgagcgtag ccaccaccg
//
LOCUS       DAONFI010000053         9119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8721_length_9119_cov_5.321933, whole genome shotgun sequence.
ACCESSION   DAONFI010000053 DAONFI010000000
VERSION     DAONFI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8721_length_9119_cov_5.321933"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLK08_02760"
     CDS             complement(<1..887)
                     /locus_tag="PLK08_02760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS52450.1"
                     /translation="MNIVSKPRIAVMIPCYNEQATVGKVVDDFREQLPEASIYVYDNN
                     STDNTATIARQHGAIVIPEPRQGKGYVIESMFGDVDADVCVMVDGDDTYPADCVHKLL
                     EPVIKGQADMAVGARLAEYTEKSFRPLHVAGNHLVRHLINRIFNADLHDILSGYRAFN
                     RKFITNVPVVSQGFEVETELTIQMLYYRLKVVEVVVPYRERPAGSVSKLNTFSDGFRV
                     LWKLFNLFRTFKPLTFFGTLCLVFFFMAVLAGLPPVVGYIQSGFTEVRRFPLAILATG
                     LMLMSCGFAFLGLILNAIN"
     gene            992..1771
                     /locus_tag="PLK08_02765"
     CDS             992..1771
                     /locus_tag="PLK08_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPS52451.1"
                     /translation="MKYANAIIFSILLAGLAACCGCPVTQSQNTPHAQKLCKSPTGED
                     YYIYVPTGYDPQKPTPLVITLHGTPPWDVALFQINEWKALAEEKNFIVVAPKLSSPQG
                     ILPVAKDERKKEVLADDAEIIALRDSLCREYNIDPKMILLTGFSAGGYAMFYTGLHHQ
                     DKFSILIARACNNDEITLDGLDAEYNEKNKSRPRIPVLFFVGKDDLQPIQDQTWMTYR
                     WLRRHGWTHKNCTHKETKGGHIRRPLTTWEMWQNYLKKYGK"
     gene            complement(1846..2061)
                     /locus_tag="PLK08_02770"
     CDS             complement(1846..2061)
                     /locus_tag="PLK08_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52452.1"
                     /translation="MPEYTRLCRTAFPDVTYKDTFLEHVKFEIAPCCVVEDEAGKEGE
                     AGNLIWFPMQPDAASRRRKQQQAQSQV"
     gene            2084..2899
                     /locus_tag="PLK08_02775"
     CDS             2084..2899
                     /locus_tag="PLK08_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPS52453.1"
                     /translation="MALVANTTSGRTVFTASVEKKTKLTYDIVMLRVRLISPDFISFR
                     PGQFVQMQIPAYEKSPTPQRRSYSIASAPEDNSHIELIIRRVPAGLGTTWIFDYLEEG
                     IEIRFSGPYGKFCMQPTDNPMLWIAGGSGLSPFRAMLYDCITRKISRPIKFYYGAVSE
                     KNLILVDELSQIAGRNGWFEFIPALSQPDADWRGAKGLITDVVARHIECCDRKNLEVY
                     LCGSPGMCDAAIDVLTGHGVGMDRIFYDKFLGQDTSEIPTPDGRAISWDPQRK"
     gene            2896..3747
                     /gene="murI"
                     /locus_tag="PLK08_02780"
     CDS             2896..3747
                     /gene="murI"
                     /locus_tag="PLK08_02780"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPS52454.1"
                     /translation="MNIQASQPIGVFDSGFGGLTVVRELLNAMPGEDIVYLGDSARVP
                     YGIKSPETIRRFAREDTSFLNGFQPKLVVVACNTATATAIDAVRRTAKAPVVDVITPG
                     AKAALKAATKGSPIGVIATQATASSGAYTRAIHAIDPSREVMTVACPLLVPIVEEGRD
                     ENDPIVTHVLCEYLCDLQRLRPGALILGCTHYPMLAGAIGKLMGPSTTLVSSAAAAAH
                     EAQLVLTQMNLLKKTSTQACGSLTCYTTDSPQRFASLVERFGGHKAKAVEYVGTDELE
                     NFSRNQK"
     gene            3744..4616
                     /locus_tag="PLK08_02785"
     CDS             3744..4616
                     /locus_tag="PLK08_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS52455.1"
                     /translation="MKHYAQIFLAAMVGLSLVAGCKGGKTPDWSDVFGKKKDPPPDVL
                     VEQLFNAQSPDTRRIAIEKLSKTYWINEEKNRELVAQMTSRSVEKSPIVRAAAVRTLA
                     KVNDPKYFDKIADAMKDDSSIVRFDAADSFELIIYPPAEDELQRLALDDDNEDVRTAA
                     CQALKNYRSDDTYRTLLRCLGDESFTVREASHESLVAMTGVDNGYDPTKWVSDPKQLG
                     KETLPAKRKVIYRKRPWWDWAGVTKETVEVKDGSQPNIMDSIDMSGGKMKMEDLSPVR
                     VEKNEKQPAKDDSE"
     gene            4613..5569
                     /locus_tag="PLK08_02790"
     CDS             4613..5569
                     /locus_tag="PLK08_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPS52456.1"
                     /translation="MKALFFDGKNLQLRTDLPAPSPSEGEVLIKVITAGVCKTDLEIL
                     KGYMGFRGIIGHEFVGVVEHGPKQWLARRVTAEINNPCHVCPTCKAGRGKHCPHRSVM
                     GIDGHDGAMAQYVTAPAENLVAIPDDVPNDSAVFIEPLAAAFEPTTRMKINRSDRVAV
                     FGDGRLGLLSAMVFNSLCDEVLLVGKHDEKLAIARGQGIKTVLAQNFSPDKSWDVTVE
                     ATGSPAGFDAAIAATRPAGTIILKSTFVAESGMNMAMVVVNEITIMGSRCGPFDAALR
                     ALREKRFDVTPLITARFSLDDGLAAMAAATSGNNIKVLIDVD"
     gene            5559..6323
                     /locus_tag="PLK08_02795"
     CDS             5559..6323
                     /locus_tag="PLK08_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPS52457.1"
                     /translation="MSIEPSNQYALTVLQCGRTVGRFKPLSRIDGLVHCVTTKRGELF
                     PPIASEGADAFKRLACDMQMPAVAWAEQVHDNTVLAVDSAGCAGQADGLVTATPGLLL
                     LGRSADCPLILAADPVRKIAGFAHASWRATIKKITVRLIDVFRELGSNPNDIVACICP
                     SAGPEKFEVGPEVFRQAVAELGPDAKNFFLAGKGDKKFFDLWAANDSQLQSAGVDFMN
                     VYSARVCTISNVETYPSYRVEGSAAQRFAATIGWKN"
     gene            6446..7822
                     /locus_tag="PLK08_02800"
     CDS             6446..7822
                     /locus_tag="PLK08_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPS52458.1"
                     /translation="MTKEKPVADILIVEDEVAHAEAIEEGLSRIGHHCSVTHDGETAV
                     AKISSTPYDIIITDLMLGGDIDGLGVLKTAREKLPAAKVILITAHSSIETCRQALQNG
                     AFDYIEKPLSLEELRIIVGRAAEMTAQRRTIRELKQRLDEHFGFGNIIGQSQAMLKVL
                     DITRRVARSDISVLLLGESGTGKELLANAIHQNSPRRDERFVAINCAGLSETLLEDEL
                     FGHVKGAYTGAATEREGRFEHADGGTLFLDEIGDMPQSMQSKLLRVLENGEVIRVGAN
                     EPKKVDVRIIAATNCDMEEKVKKHAFREDLYFRIKGATIEIPPLRNRRDDIPLLIEHF
                     IQQANKRHNMRIKNADPEVRRLLMSYNWPGNVRQLKNVVENMVVLSMTDRLNVEDLPA
                     EIHTPPSGINDCNMGAMAGISLQEAERLLIVNTLKMVEGNREKAADILGIGERTLYRK
                     IKEYNLDV"
     gene            7840..>9119
                     /locus_tag="PLK08_02805"
     CDS             7840..>9119
                     /locus_tag="PLK08_02805"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HPS52459.1"
                     /translation="MADDFNNVNYDGIFNKKPKSNPLETVSSIGSRIGQIIFGTMFGG
                     IALSAAIMLIFFSYKDIVKNLNHQTAIATIASIDGSLEDSEITINWSFVIDDKTYAGR
                     KTANESSHQKLYDTFSQYKTGEQIIVSYDPANPQNNDPYKPFNPAILFMLLFTVPFIA
                     TGFMIVTGKLKKEKSVEPGTKDYAAVICIIGLFLLCCPAAFLFAFLGEKTSPYISISL
                     FLAYVILIPFIIRSINCKLKKTTHARQELVQFQTESHVENKSCEQAGNLSHSSFKKEV
                     CPLLFFSLVWWFLLGIFTVIMGYQAHKYLTAKATYERTTGTMLSSRVAEHYSNNSTTY
                     SPEIKYIYYVNGKKYIGKKYDVTGGSSSGSWAYNIVRKNPKGQKVTVYYDPANPSKAV
                     ISIAPPEMLKAFLFFLQPFFAAGVLIILATIKTT"
BASE COUNT         2478 a         2465 c         2283 g         1893 t
ORIGIN      
        1 cagttaattg cgttaagtat caggcccaga aacgcaaacc cgcaagacat cagcatcagc
       61 cccgtagcca gtatcgccag cggaaaacgt ctgacttccg tgaaaccgct ctgaatataa
      121 cccaccaccg gcggcagccc cgcaagaacg gccatgaaga aaaataccag acataacgtt
      181 ccaaaaaaag tcagcggctt gaatgtgcgg aaaagattga aaagtttcca caacacgcga
      241 aagccgtcgc tgaaagtgtt cagtttcgaa acgctgccag ccggccgttc acgatacggc
      301 acgacgacct ccacaacctt cagccggtaa tacaacatct gtatcgtaag ctccgtttca
      361 acctcaaatc cctgggaaac caccggcacg ttcgtaatga atttgcgatt gaacgcgcga
      421 tagccggaaa gtatgtcgtg gagatcggca ttgaaaattc tgttgataag atgtctgaca
      481 agatgattgc cggctacatg cagggggcgg aaactttttt cggtatactc agccagccgc
      541 gcaccgacgg ccatatcagc ctggcctttg atgaccggct caagcagctt gtgaacgcaa
      601 tccgccggat acgtgtcgtc gccatccacc atgacgcaca cgtcggcatc cacgtcgccg
      661 aacattgact ctatcacgta acccttgccc tgcctgggtt cgggaataac tatcgccccg
      721 tgttgacggg cgatggttgc cgtgttatcc gtagagttgt tgtcatatac gtaaatcgac
      781 gcttccggca gctgttcgcg aaagtcgtca acaaccttgc cgacggtagc ctgctcgtta
      841 tagcacggta tcattactgc gattctgggt ttggatacta tattcatagg cgatatggta
      901 cacgcggggc ggcacaaatc caagagaaaa cacgactgac tttcagggcg gaatattgta
      961 taatgcggac ttcgtttttc aggatatgaa catgaaatat gcaaacgcaa taattttttc
     1021 gatattgctg gccggcctgg cagcctgctg cggctgcccc gtcacgcaat cacagaatac
     1081 gcctcacgct cagaaactct gcaaaagccc gaccggcgaa gattattaca tctacgttcc
     1141 gaccggttac gacccccaga agccaactcc tcttgtgata accctgcacg gcacgccgcc
     1201 gtgggacgtg gcgttattcc agataaatga gtggaaagcg ctggccgagg aaaagaattt
     1261 cattgtcgtt gcgccaaaac tcagcagccc gcaaggaatc ctgcctgtcg ccaaagatga
     1321 acgtaaaaag gaagtactcg ccgacgacgc tgaaatcata gcattgcggg actcgctgtg
     1381 ccgcgaatac aacatcgacc cgaagatgat tctgctgacg ggtttttccg ccggcggtta
     1441 tgccatgttt tacaccggcc tgcatcacca ggacaagttc agcattctca tcgcccgcgc
     1501 atgtaataac gacgagataa cgctcgacgg cctcgacgcc gaatataacg aaaaaaacaa
     1561 atccaggccc cgcattccag tgctgttttt cgtcggcaag gacgaccttc agccgataca
     1621 ggaccaaaca tggatgacgt atcgctggct gaggcggcac ggctggacgc acaaaaactg
     1681 tacgcacaaa gagactaagg gcggccacat ccggcggccg ctgaccacat gggaaatgtg
     1741 gcagaattac ctcaagaaat acggaaaata aacaaaaata acagataata aaatcctggc
     1801 ggcatgccgc gatttcaata gcggaatcct ccggtttaga gcatattaaa cttgactttg
     1861 cgcttgttgc tgtttgcggc gtcggctggc tgcgtcaggc tgcatcggaa accatataag
     1921 gttgcctgct tcgccttcct tgccagcctc gtcctcaacg acgcaacaag gcgcaatttc
     1981 aaatttaaca tgctctagaa acgtgtcttt ataagtaacg tcgggaaaag cggttcggca
     2041 aagtcttgta tattccggca tcggagagta tcctttaggt cttatggctt tagtcgcaaa
     2101 tacaaccagc gggcgaacgg tttttaccgc atccgtcgaa aaaaaaacaa agctgaccta
     2161 tgacatagtc atgctgcgag tgcggcttat ttcgccggat ttcataagtt tccggcccgg
     2221 ccagttcgtg cagatgcaga tacccgcgta tgaaaaatcc ccgacgccgc agagacgttc
     2281 gtattcgatt gcatccgcac ctgaagataa cagccatata gagttgataa ttcgccgcgt
     2341 gccggccggc ctgggtacta cgtggatatt cgattatctt gaagaaggca tagaaatccg
     2401 attctccggc ccatacggaa aattctgtat gcagccgacc gacaacccca tgctctggat
     2461 agccggcggt tcaggactca gcccgttccg tgcgatgctt tacgattgca taacccgcaa
     2521 aataagtcgt ccgataaaat tttattacgg cgccgtcagc gaaaaaaatc tcatactcgt
     2581 tgatgagctt tcgcaaatcg ccggcagaaa cggctggttt gaattcatac cggctctttc
     2641 acagccggac gcagactggc gcggcgccaa aggccttata acagacgttg tcgcaagaca
     2701 tatcgaatgt tgcgacagga aaaatctgga agtttacctg tgcggttcgc ctggcatgtg
     2761 tgatgcggct atagatgtgt taaccggcca cggcgtcggc atggacagga ttttttacga
     2821 caaatttctc ggccaggata cgtcggaaat tccgacgccc gacggcagag caatttcatg
     2881 ggatccgcaa cgaaaatgaa tattcaggca tcacaaccaa taggcgtatt cgactccggc
     2941 ttcggcggat tgacggtagt acgggagttg ctcaacgcca tgcccggcga ggacatagta
     3001 tatttgggcg attccgcacg tgtgccatac ggcatcaagt ctcctgagac cattcgccgc
     3061 tttgcccgcg aagacacttc gtttctgaac ggctttcagc cgaaactcgt agtcgtagcc
     3121 tgcaataccg ccaccgccac cgccattgac gccgtccgcc ggacggctaa agctccggtc
     3181 gtggacgtga taacgcccgg cgcaaaagcc gccctcaagg ctgcgacaaa aggctctccc
     3241 atcggcgtca ttgccacgca ggctaccgcg tcgagcggcg catacacgcg ggctattcac
     3301 gcgatagacc cgtcgcgcga ggtcatgacc gtagcctgtc cgctgcttgt acccatcgtc
     3361 gaggaaggcc gtgatgaaaa cgacccaata gtcacgcatg tgctctgcga atatctgtgc
     3421 gatttgcagc ggctgcggcc aggcgcactg atactcggct gtacgcatta tccgatgctc
     3481 gcaggcgcaa taggcaaact catgggccct tcaaccacgc tggtcagctc cgcggcagcg
     3541 gcagctcacg aagcacaact cgtccttacg cagatgaatc tgcttaaaaa aacttcgacg
     3601 caagcctgcg gcagtctgac gtgttataca accgacagcc cgcaacggtt cgccagcctc
     3661 gtggaacgat tcggcggcca caaggcaaaa gccgtcgaat acgtcggaac ggatgaactt
     3721 gaaaacttca gcaggaatca aaaatgaaac attatgcaca aatatttctg gcagcgatgg
     3781 taggactgtc gttggtagcc ggctgcaaag gcggcaaaac gcccgactgg agtgacgtgt
     3841 tcggcaaaaa gaaagacccg ccgccggatg tgcttgtgga acagcttttt aatgcacaat
     3901 caccggatac gcgacgcata gcgattgaaa aactctccaa aacgtactgg ataaacgaag
     3961 aaaaaaatcg cgaattagtc gcgcagatga ccagccggtc ggtcgagaaa tcgcccatag
     4021 tccgcgcggc agccgtccgt acgctggcga aagtcaacga cccgaaatat tttgataaaa
     4081 tcgccgacgc catgaaggac gacagttcta tcgtgcgatt cgacgcagcc gattcatttg
     4141 aactgataat ctatccgccg gctgaggatg aactccagcg gctggcgctg gacgacgata
     4201 atgaagacgt ccgcacagca gcctgccagg cattaaaaaa ttatcgctca gacgatacct
     4261 atcgtaccct tctgcgatgc ctgggcgacg aatctttcac cgtgcgggag gcgtcgcatg
     4321 aatctctcgt ggcgatgacc ggcgtggata acggttacga cccgaccaaa tgggtctccg
     4381 accccaaaca gctcggcaag gaaacactgc cggctaaacg caaggtcata taccgcaagc
     4441 gaccctggtg ggactgggcc ggagtaacaa aggaaacggt cgaagtgaag gacggttcgc
     4501 agccgaatat aatggacagc atagacatgt ccggcggaaa aatgaaaatg gaagatctct
     4561 cgcctgttcg cgtagagaaa aacgaaaaac agccagcgaa agacgactcg gaatgaaagc
     4621 attattcttc gacggcaaaa atctgcaact gagaaccgac ctgccggcgc catcgccgtc
     4681 tgaaggtgaa gtgcttataa aagtcattac cgccggcgtt tgcaagaccg acctcgaaat
     4741 actcaagggt tacatgggat tcaggggcat catcggccat gaattcgtcg gcgttgtgga
     4801 acacggccca aagcagtggc tcgcgcgtcg cgtgacagcc gaaataaaca atccatgtca
     4861 cgtctgcccc acatgcaagg ctggccgcgg caaacattgc ccacatcgct cagtaatggg
     4921 aatagacgga cacgacggtg caatggcaca gtatgtcacc gcacctgccg aaaatctcgt
     4981 cgccattccg gatgacgttc cgaacgactc cgcagtattt atcgagccgc tggcggcagc
     5041 tttcgagccg actacccgca tgaaaatcaa tcgcagcgac cgcgttgccg tcttcggcga
     5101 tggccgactg ggcctgctaa gcgcaatggt tttcaactcg ctgtgcgatg aagtcctgct
     5161 cgtcggcaaa catgacgaaa aactcgcaat cgcccgcgga cagggcataa aaactgtttt
     5221 agcccaaaat ttctcgccgg acaaatcgtg ggacgtaacg gtggaggcta ccggctcgcc
     5281 agccggcttt gacgccgcca tcgccgccac acgccccgcc ggcacgataa tcctcaaaag
     5341 tacattcgtc gccgaaagcg gcatgaacat ggcgatggtc gtcgtgaacg aaatcacgat
     5401 tatgggttcg cgatgcggcc cgttcgacgc ggcacttcgt gcgttgcggg aaaagcgttt
     5461 cgacgttacg ccgctgataa cagcccgttt cagcctggac gacggcctcg ccgccatggc
     5521 cgccgcgaca tccggcaata atataaaggt actcatagat gtcgattgag ccgagcaatc
     5581 aatatgcact gacggttctg cagtgcggca gaaccgtcgg ccgattcaag ccgctgagcc
     5641 gcatcgacgg cctcgtacac tgcgtaacca caaaacgcgg cgaacttttt ccgccaatcg
     5701 cctcggaggg agcagacgct tttaaaaggc tagcctgcga catgcagatg ccggctgtcg
     5761 cctgggctga gcaggtgcat gacaacaccg ttttagccgt cgattctgcc ggctgcgcgg
     5821 gccaggctga cggcctggtg acggcaacgc ctggccttct gctgcttggc cgcagcgcag
     5881 attgccccct gatattagcc gccgaccctg tcagaaaaat agccggcttc gcacacgcat
     5941 catggcgtgc aacaataaaa aaaatcacag tgcgtcttat agacgttttc cgcgaactcg
     6001 gcagtaatcc caatgatatc gtagcatgca tctgcccgtc ggcaggcccg gagaaattcg
     6061 aggtcggccc ggaagttttc cggcaggctg tagcagaact cgggccggat gcaaaaaatt
     6121 tcttcctcgc cggcaagggc gataaaaaat ttttcgacct ctgggcggca aacgattcgc
     6181 agttgcaatc cgccggcgta gacttcatga acgtatactc cgcccgcgtc tgcacgatat
     6241 ccaatgttga gacttatccc agctaccgcg tcgaagggtc agccgcccaa cgcttcgccg
     6301 caacgatagg ctggaaaaac tgattgtaca agccgccgtt ttgcttaaaa tgaagaattt
     6361 acagcagttc tcaaaaatct tttcattttg gaaactgcgt tagagcataa ttgatgcatt
     6421 catcagatat ttcgagacgg acaaaatgac caaggaaaaa ccagttgccg acattctgat
     6481 agttgaagac gaggtcgccc acgccgaggc aatcgaggaa ggcctttcgc gtataggcca
     6541 tcattgcagc gttacccacg acggcgaaac ggcggtagcc aaaatttcgt ccacgccgta
     6601 cgacatcatc ataaccgatc tcatgttggg cggcgatatc gacggcttgg gcgtattgaa
     6661 aacagccagg gaaaaactcc cagccgccaa ggtcatacta ataacggctc attcgtccat
     6721 cgagacctgc aggcaggctt tgcagaatgg cgctttcgac tatatcgaaa agccgctcag
     6781 cctcgaagaa ctccgcataa tcgtaggccg agccgccgaa atgacggcac aacgacggac
     6841 aatccgcgaa ttgaaacagc ggcttgacga gcatttcggg ttcggcaata taatcgggca
     6901 gtcgcaggct atgctcaagg tactggacat cacccggcgc gtagcccgca gcgacatatc
     6961 cgtactgctg ctcggcgaaa gcggcaccgg caaggaactg ctggctaacg cgatacatca
     7021 gaattcaccg cgacgcgacg agcgtttcgt ggccatcaac tgcgctggcc tgagtgaaac
     7081 gctgctggag gatgaactct tcggccacgt caagggggcc tacaccggcg cagccaccga
     7141 gcgggaaggc cgattcgaac atgccgacgg cggtacgcta ttcctggacg aaatcggcga
     7201 tatgccccag tcaatgcagt ccaaactgct gcgagtgctg gaaaacggcg aagtcatccg
     7261 cgtcggggcc aacgagccca aaaaagtaga cgtgcgaatc atcgccgcca ccaactgcga
     7321 catggaagaa aaggtcaaaa aacatgcttt tcgcgaagac ctctacttcc gcataaaagg
     7381 cgcgacgatt gaaattccgc cgctccgcaa caggcgcgac gacattccgc tgctcatcga
     7441 acatttcatt cagcaggcga acaaacgtca caacatgcgg ataaaaaacg cagaccctga
     7501 agtccggcga ctgttgatga gctataattg gccaggaaac gtcaggcagc tgaaaaatgt
     7561 ggtggaaaat atggtagtac tgtccatgac cgacaggctc aacgtagaag acctgccggc
     7621 ggagattcac accccgccgt cgggcattaa cgattgcaat atgggtgcga tggccggcat
     7681 aagtctccag gaggcagaac ggctgttaat cgtcaacacg ttaaaaatgg tcgaaggtaa
     7741 ccgtgaaaaa gccgccgaca tcctcggcat aggcgaacgc actctctacc gcaaaataaa
     7801 agaatataac ctggacgtgt gaaccggatt agggcaaaca tggccgacga ttttaacaat
     7861 gtaaattatg acggaatctt taataaaaaa ccgaaatcaa atccgcttga aacggtttcg
     7921 tcaataggtt cgcgcatcgg ccagataatt ttcggcacaa tgttcggagg aattgcacta
     7981 tcggcggcga taatgcttat cttcttttca tataaagata tagttaaaaa cctgaatcac
     8041 cagaccgcaa tagctaccat cgcatccatt gacggttcgc tggaagattc cgaaatcaca
     8101 ataaattggt cattcgttat tgatgataaa acatatgctg gcagaaaaac cgcaaacgaa
     8161 tcgtcgcatc aaaaacttta cgacacattc agccaataca aaaccggcga acaaataatc
     8221 gtttcatacg acccagccaa tcctcagaat aatgacccgt ataaaccatt caatccggcc
     8281 atactattca tgctgctgtt caccgtacca ttcatagcca caggattcat gatagtcaca
     8341 ggcaaactga agaaagaaaa atccgtagaa ccaggaacaa aagattatgc agcagttatt
     8401 tgcattatcg gactgttttt attgtgctgt ccggcagcgt ttctgtttgc ttttttaggt
     8461 gaaaagactt cgccctatat atccatatct cttttcctgg catatgtgat attgatacct
     8521 ttcattatca ggtccatcaa ctgcaaactc aaaaaaacaa cccacgccag acaggaactg
     8581 gtacaattcc agacggaatc ccatgtggaa aataaatcct gcgaacaggc aggtaatctg
     8641 tcgcattcat ctttcaaaaa agaagtctgc ccattactgt ttttttcgct tgtgtggtgg
     8701 tttctactcg gaatattcac ggtgataatg ggatatcagg cacataaata tcttactgcg
     8761 aaagcaacat atgagcgtac aaccggcacg atgctgtcat caagggtcgc cgagcactac
     8821 agcaacaatt cgacaacata ttcaccggaa attaaataca tctattatgt caacggcaaa
     8881 aaatacatcg gtaaaaagta cgatgtcaca ggcggaagct ctagcggcag ttgggcatac
     8941 aacatcgtac gtaaaaatcc aaaaggccaa aaagtcaccg tatattacga ccctgcaaat
     9001 ccgtcgaaag ccgtcatttc aatagcccca cctgaaatgc tcaaggcatt cctgtttttc
     9061 cttcagccgt ttttcgcggc aggtgtcctg attattctgg ccaccatcaa aacgacaaa
//
LOCUS       DAONFI010000054         9076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8777_length_9076_cov_4.858552, whole genome shotgun sequence.
ACCESSION   DAONFI010000054 DAONFI010000000
VERSION     DAONFI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8777_length_9076_cov_4.858552"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1568)
                     /locus_tag="PLK08_02810"
     CDS             complement(21..1568)
                     /locus_tag="PLK08_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPS52460.1"
                     /translation="MPSTQPPQIAVIAGGGPAGLTAAYELLCNKDANIKPVVFEKLPI
                     MGGISRTIDYRGNRMDIGGHRFFSKSDAVMDWWLKILPLQNENRNPEKIDEVMLQRSR
                     KSRILWMRKLFDYPLTLGGSTLRKLGILRTLRIGLSYIRAAIFRRKPEVTLEDFFINR
                     FGVVLYKLFFRDYTEKVWGMPCNKISAAWGAQRVKGVSVTKALLHAVRQIFVFKKTSS
                     DDIRQKNVETSLIDRFLYPKFGPGQLWELVAKRVTEMGGEILRNWEIVGIKTDGQIVT
                     GVCARGADGEIKNFECDYLLSSLPLKDLVESMDCDVPAEIGEIATGLAYRDFVTVGLL
                     AKDFLLKDSENPPKIVADNWIYIQESDIKAGRLQLFNNWSPYMVARADQVWIGLEYFC
                     QEGDEFWNRSDAELLELAKCEMDKCGIIRPADVLDGVVVRVQKAYPAYFGTYDRIDVL
                     RQYLDSYKNLFCIGRNGQHRYNNMDHSMLTAMQAVRNIIGGSADKSNVWAVNTEREYH
                     ESKSTEQ"
     gene            2089..3345
                     /gene="aroA"
                     /locus_tag="PLK08_02815"
     CDS             2089..3345
                     /gene="aroA"
                     /locus_tag="PLK08_02815"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPS52461.1"
                     /translation="MKKLSGKFTPPGDKSISHRIALFALAAEGSAAVTNYSPCDDCTS
                     SLNAVKKLGCTITQAGDALIIHGAANRHLNSGHIDCGNSGTTMRLLCGLLAGRIGRFE
                     LDGDQYLRRRPMERIVSPLKMMGGELQTTNGKCPITVTGESLEGIEYELPVASAQLKS
                     AILLAGLTAKGTTTIIENIPSRDHTERMLKAFGAEIFGDKRRIILNPSQIVLPEKFAV
                     PADASSAAFFLCTAAITPGSDVTATGMLLNPTRCAFLDVLKRMGADVTVTHTGDSPEP
                     VGDVRVKYSPNLLPTTIEPAEIPLVVDEIPILALTACCASGCSVFKNAGELRVKECDR
                     LAAIQSQLNRMGAEIEITPDDSLIITGKTEFKGGIKCNSMGDHRIAMVLRCASLLADD
                     DIEISDEKCVSVSYPNFHSELERLTQ"
     gene            3342..4160
                     /locus_tag="PLK08_02820"
     CDS             3342..4160
                     /locus_tag="PLK08_02820"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPS52462.1"
                     /translation="MMKFCVISDDRAFKSRSPEMFRKVFSTLGMDGQYVPLRTLPEEV
                     GHTVKSLGEKGFNGANVTVPYKQTVMTFLDEIADDAIAAQAVNTIQVRFDGKLIGYNT
                     DVRGFLDAMSLAGVDMTGKKVLLIGTGGAARGILVALNKASAAEIILAGRTPEKTRAL
                     AQWAGVGNVDLQTAFMQHADAQILINASAVSSQEEAPQMADMLRKVVLKNCEMVCDIN
                     YARKNNFWQTMAARSDAKFSDGLPMLACQARRSLEYWTAQTQPCEPFLNALGIK"
     gene            4334..5908
                     /gene="aroB"
                     /locus_tag="PLK08_02825"
     CDS             4334..5908
                     /gene="aroB"
                     /locus_tag="PLK08_02825"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPS52463.1"
                     /translation="MKKILRNIYLVGFMGSGKSAVASELAALLRRRCVDMDFEIESLF
                     EKPITKIFELAGEERFREIESRLLKCIASANRAVVSTGGGIVVRNENIRRMRHSGLVV
                     FLRASFDECRKRVGPAARKTRPLWQNPAAAKKLFSARVKLYKQAAELVVDTDGISPRQ
                     AAEIIAKKVLSQLDCEFTASLGGEKSQVISTADAPAVVSKNLAGQRVVLLTDSNVAKL
                     HLERYNAALPDCHVHILPPGERIKTLDSAKRFYQKLLELKINRDDVLLAVGGGTVTDF
                     GAYVAFTFKRGIKFVLVSTTLLGCVDAAVGGKAAVNLENTKNIIGCFTVPQLVVLDAW
                     AFSTLPAQRVREGLVEAYKTGLIYNAQLAEIIEKNLDALLDGDTVLLSLIAAESARTK
                     AAVVSQDFRESSLRAILNFGHTFGHAVEGFNKYKASHGYCIATGMAVAVELSLARGLI
                     SSQLADRIKTTLNSIVPKMPRCPSLAQAQTIMENDKKIRQGKLVFVLLEGMGKTRMVS
                     DISRSELSRAIKKQEI"
     gene            5910..6962
                     /gene="aroC"
                     /locus_tag="PLK08_02830"
     CDS             5910..6962
                     /gene="aroC"
                     /locus_tag="PLK08_02830"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPS52464.1"
                     /translation="MSSSTGTIFRVTTFGESHGAALGAVVDGVPAGLKIDMAFLQQHM
                     NRRRPGTSKFVSPRKEPDKLEILSGVDSQCVTLGTPLTLIVRNTDVHSEDYASLRNTY
                     RPGHADFTYHKKYGVAPQPGGGRSSGRETLARVAAGTIARLILGEQVKIFSYCTAISG
                     VVAKVTDIPFAEMHPLRFADPALAAKAEQAVLAANKEGNSVGGIIELLICGVPAGLGE
                     PVFEKLDAALAGAVMSIGAVKGIEFGAGFRFPEMTGQQANDQMSPDGFESNNAGGIIG
                     GISTGQNIIMRLAVKPTPSIAVPQKSITFDGKAATVTTTGRHDPCVAPRVAIVAEAMA
                     AIVLADALLCQKTRIL"
     gene            7560..8600
                     /locus_tag="PLK08_02835"
     CDS             7560..8600
                     /locus_tag="PLK08_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52465.1"
                     /translation="MKHGWLTCLALAGVAMLVGGCDMDFSKVDNPEYAAWKGFPVGSG
                     YEFEGMLQTSAENPKRIQISANLMVFDLTQAVLKRRAQIFNPGGSGQLEFTDVIIPAR
                     VKKNDCLLTQDGMQDNFRGWDSVLVGEKLYDCEVHYYVVKRDGEILGIKTHGETGIKI
                     WQCKKIPGGMAKIEVTGLSPSCIFSLSGQIVNVGMARITISPPGQATETETHPAKPAN
                     NPAGTDLEKAITSPEHLPIMPQPEKKVETSGLNTSSATTEEDDSAPKVILPATNPSDK
                     KAFEEVPQKIEPVEKPVQRQPDPQLLSEVQEEDSPTSEPSSIKPVKLDVPERLDEDTA
                     PLPKFAAPDDSK"
     gene            8688..>9076
                     /gene="aroQ"
                     /locus_tag="PLK08_02840"
     CDS             8688..>9076
                     /gene="aroQ"
                     /locus_tag="PLK08_02840"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPS52466.1"
                     /translation="MKIRVINGPNLNMLGTREPEIYGKTTLADIEKLLRTTAEECEVE
                     LEFVQSNHEGEIVTAIQQSRSADGIILNAAAYTHTSVAIRDAISAVAVPTVEIHLSNI
                     HAREDFRHHSLIAPVCKGVIAGFGVDS"
BASE COUNT         2497 a         2328 c         2214 g         2037 t
ORIGIN      
        1 gaaaaataat aacgaatatt ttattgctcg gtcgattttg actcatgata ttcccgttca
       61 gtattcacag cccacacgtt tgatttatct gcactgccgc cgataatatt tctgacggcc
      121 tgcatggcgg tgagcatgga atgatccatg ttattgtatc tatgttggcc gtttctgccg
      181 atacagaaaa gatttttgta cgaatccaga tactgacgca acacgtctat tcggtcatat
      241 gttccaaaat atgccggata agccttctga acgcgaacca ccacgccgtc aagcacatcc
      301 gccggccgaa tgattccgca tttgtccatt tcacactttg caagctccag cagttccgcg
      361 tcgctgcgat tccagaattc atcgccttcc tggcagaaat attccaaccc tatccaaacc
      421 tggtcagctc tggcaaccat atacggactc cagttattga acaactgcag gcggccggct
      481 tttatgtcgg actcctgaat gtatatccag ttgtcggcaa cgattttggg gggattttct
      541 gagtctttga gtagaaaatc tttcgccagc aggccgaccg tgacaaaatc acgatacgcc
      601 aggccggttg cgatttcccc aatctccgcc ggcacgtcgc aatccatcga ttcgacaaga
      661 tccttcagcg gcagcgacga gagcagataa tcgcactcaa aattttttat ctcgccgtcc
      721 gctcccctgg cacatacgcc ggtaactatt tggccgtcgg tttttatgcc gacaatctcc
      781 caattacgca aaatttctcc gcccatttcc gtcacgcgtt ttgcaaccag ttcccaaagc
      841 tggcccggcc cgaatttagg atacaaaaat ctgtcgataa gagacgtttc cacatttttt
      901 tgtcgaatat cgtcggaaga ggttttttta aagacgaaaa tttgtctgac ggcgtgaagc
      961 agggcttttg ttaccgatac gcctttaact cgctgcgccc cccacgctgc ggaaatctta
     1021 ttgcacggca tgccccagac cttctccgtg taatcgcgga agaacagctt gtataaaacg
     1081 acgccaaaac gattgatgaa aaaatcctcc agcgtcactt caggcttgcg tcgaaatata
     1141 gcagcacgaa tatagctcag gccgattctc aatgttcgta aaatccccag ttttcgcaac
     1201 gtactgccgc cgagcgtcag cggataatca aaaagtttcc gcatccagag aatgcgggac
     1261 ttcctgctgc gctgcaacat gacttcatcg attttttcgg gattgcgatt ttcgttctgc
     1321 aacggcagaa ttttcagcca ccagtccatg accgcgtccg acttggagaa gaaccggtgg
     1381 ccgccgatat ccatgcgatt gcccctgtaa tcaatcgttc gcgatatgcc gcccataatc
     1441 ggaagttttt caaaaacgac aggtttgata ttcgcatctt tattgcaaag cagttcgtag
     1501 gctgccgtca atccagccgg ccctcctccg gcaattacgg ctatttgcgg cggttgagtt
     1561 gatggcattg tttccaaaac ctttcatatg aaaaggccag taaatcattc aaaaacttcc
     1621 tgttgacaat gataggctca gcagtagata tgtcaataaa atctccgtta gtcattaaga
     1681 gcgtctaaca aaatgagatg tcgcaccgag gcgtagttgg ttgattggct ggcaaggaag
     1741 gcgaagcaga tagccttata gggcttgcga tgcagcctga cgccgccaga cattcaacca
     1801 acaagccgaa tgcaatatcg ttttgttagg cgttctaagt caaaaagcga taaaattctt
     1861 gactggcggc tggtcgggga atatccttgt catcgtgaaa aaaggctgga aaaacccgtt
     1921 taaagtcaat ttttcggcat gttaataaga gcggtttcgt cgtgcgacac ggctggtccg
     1981 gcaatcgcga caggccttgt cgcttcggct cgacataaaa aggttgagaa accctcgtcg
     2041 cacggattgt gagcgttttt ttgtcttgac gcatattttg agaaatttat gaaaaaacta
     2101 tcgggaaaat tcacgccgcc gggcgataaa tccatcagtc accgcattgc attgtttgca
     2161 cttgcggcgg aaggctctgc cgccgttaca aattattcgc catgcgacga ttgtacgtcg
     2221 tctttaaatg ccgtaaaaaa actcggttgt acaatcacgc aagccggcga cgctctcatc
     2281 attcatggag cggcaaacag acacctgaac tccggccata tcgactgcgg caattccggc
     2341 acaaccatgc gcctgctatg cggcctcctc gccggcagaa tcggacggtt tgaacttgac
     2401 ggcgaccaat atctgcgtcg ccggcctatg gaacgtatcg tctcgccgtt gaaaatgatg
     2461 ggcggcgaac tacagacaac caacggcaaa tgcccgataa ccgtcactgg cgaatctctg
     2521 gaaggaatcg aatacgaact gccggtcgcc agcgcacaac tgaaaagtgc gatactgctc
     2581 gccggcttga cggctaaagg tacgacgacg attatcgaaa acatcccctc ccgcgaccat
     2641 accgagcgga tgctcaaggc attcggcgcc gaaatttttg gcgacaaacg ccgcataata
     2701 ttgaatccgt cgcaaatcgt tctgcccgaa aaattcgccg tgccagccga cgcgtccagc
     2761 gcggcgttct tcctgtgcac tgccgccata acacccggca gcgacgtaac agcaaccggc
     2821 atgctgctca acccgacgcg atgcgcattt ctggacgtac tcaaacgcat gggcgcggac
     2881 gtcaccgtaa cgcataccgg cgattcgcct gaacctgtcg gcgacgtgcg cgtaaaatac
     2941 tccccaaacc tgctgccgac gacaatcgag ccagccgaaa tcccgctggt cgtcgacgaa
     3001 attccgatac ttgcattgac cgcctgctgc gcatcgggat gcagcgtctt caaaaacgcc
     3061 ggcgaattgc gggtaaaaga atgcgaccgg ctggcagcta tccagtccca actaaaccgc
     3121 atgggtgcag aaatcgaaat aacgccagac gacagcctta taatcaccgg caaaacagaa
     3181 tttaaaggcg ggataaaatg caattccatg ggcgaccacc ggatagccat ggttctgcga
     3241 tgtgcatcgc tgctggctga tgacgacatc gaaatttcag atgaaaaatg cgtcagcgtt
     3301 tcttatccga attttcactc cgagctggaa aggctgacgc aatgatgaag ttttgcgtaa
     3361 tcagcgacga cagagccttt aaaagccgct caccagaaat gttccgcaag gttttttcaa
     3421 cactgggcat ggacggtcaa tatgtccccc tgcgcacact gcctgaggaa gtcggccata
     3481 ccgtaaaatc actcggcgaa aaaggtttta acggtgcaaa cgtcaccgtc ccgtacaagc
     3541 agacggtcat gacttttctg gatgaaatcg ccgacgacgc catcgccgca caggctgtca
     3601 acacaataca ggtacgattc gacggcaaac taataggcta taacaccgac gtgcgcggat
     3661 ttttagacgc catgtcgctc gccggcgtag atatgaccgg caaaaaagtc ctgctcatcg
     3721 gcacaggcgg tgcggcgaga ggaatactcg tagccctgaa caaagcatcg gccgccgaaa
     3781 tcatactagc cggcagaaca cctgaaaaaa cacgagcact ggcacaatgg gccggcgtcg
     3841 gaaacgtaga cctgcaaacc gccttcatgc aacatgccga cgcacaaata ttgataaacg
     3901 cttcagccgt cagctctcag gaagaagctc ctcaaatggc cgatatgctc cggaaagtcg
     3961 ttttgaaaaa ttgcgagatg gtctgcgaca tcaattatgc tcgcaaaaac aatttctggc
     4021 agacgatggc tgccagaagc gacgccaaat tttccgacgg cctgccaatg ctggcctgcc
     4081 aggcaaggag aagtcttgaa tattggacag cccaaactca accttgcgaa ccttttttaa
     4141 atgcactggg cattaaatag tttattatgg caaataaccc tgcaaccttt tttaaatgca
     4201 ttgggcatta aatagtttat tgaagcaaat aaccttgtaa cctttttaaa tgcaccggtt
     4261 attaatatag tttgaataaa tttgaaatgc tccatatttt aaactctgtt tttcataaaa
     4321 caggagcgaa cagttgaaaa aaattttgcg caacatatat cttgtcggat tcatgggcag
     4381 cggaaaaagt gccgtagcat cggagcttgc cgcactattg cggcggcgtt gcgtcgatat
     4441 ggacttcgaa atagaatcgc tatttgaaaa accaatcacg aaaatttttg aattagccgg
     4501 cgaagagcgt tttcgtgaaa tagaatctcg gctgctcaaa tgcatcgcca gtgcgaatcg
     4561 cgcggtagtt tcgaccggcg gcggcatagt ggtgcggaat gaaaatatcc gccgaatgcg
     4621 gcatagtggt cttgttgtgt ttctgcgagc atcttttgat gaatgtcgta aacgcgtcgg
     4681 gccggcagcg cgcaagacaa ggccgctatg gcaaaatccg gcagctgcta aaaaactttt
     4741 ttccgccagg gtcaaactat ataaacaggc ggcggaatta gtcgttgata cggacggaat
     4801 ttcaccgcgg caggcagcgg aaataatagc caaaaaggtg ctttcgcaac ttgactgcga
     4861 atttacagcc tcgctcggag gtgaaaaatc gcaggtaata tccaccgccg acgcaccagc
     4921 cgtcgtctcg aaaaacctcg ccggccaacg ggtagtgctt ctcacggatt caaatgtcgc
     4981 aaaacttcac ctcgagagat ataacgccgc cctgcccgac tgtcacgttc atattcttcc
     5041 gccaggcgaa agaataaaaa cgctcgatag cgccaagagg ttttatcaaa aactgcttga
     5101 actcaaaatc aatcgcgatg acgtattgtt agccgtcggc ggcggaacgg tgacggattt
     5161 cggcgcatat gtcgcgttca ctttcaagcg cggaataaaa ttcgtactcg tcagcaccac
     5221 gttgctgggc tgtgtagatg ctgctgtcgg cggaaaagcg gcggtaaatc tcgaaaatac
     5281 gaaaaacatc atcggctgtt ttactgtgcc gcagctcgtg gttctggacg cgtgggcttt
     5341 ttccactctg ccggctcagc gcgtacggga aggtctcgtg gaagcctaca aaacaggcct
     5401 gatttataat gcgcagctgg ctgaaattat agaaaaaaat ctcgacgcgc tgcttgacgg
     5461 cgatacggtt ctgctcagcc tgattgcggc ggaatcggct cgcaccaaag ccgctgttgt
     5521 ttcacaggat ttcagggaat cgtcgttacg ggcgattctg aattttggcc atacgtttgg
     5581 ccacgccgtc gaaggtttta ataaatataa ggccagccac gggtactgta tagcaaccgg
     5641 catggctgtc gcggtcgaac tttcactggc gcgaggactg atatcgtcgc agcttgccga
     5701 cagaataaaa acgaccctga acagtatagt gcccaaaatg ccgcgttgcc cctcattggc
     5761 tcaggcgcag acaataatgg aaaacgacaa aaaaatccgc cagggtaaac tggttttcgt
     5821 gttgctggaa ggcatgggca aaacgcggat ggtcagcgac atcagccgca gtgaactgtc
     5881 gcgagcaata aaaaaacagg agatatgaca tgtcgagttc aacgggcacg attttcagag
     5941 taaccacttt tggcgaatct cacggcgcgg cgcttggcgc ggtggtggac ggcgtaccgg
     6001 caggattgaa aatcgacatg gccttccttc agcagcacat gaacagacgc cggcctggca
     6061 caagcaaatt cgtctcgccc cgcaaagaac ctgacaaact tgaaatactc tccggcgtgg
     6121 attcgcaatg cgttacgctc ggcacgccgt tgacgctgat tgtacgcaat accgacgttc
     6181 acagcgaaga ttacgcttct ttacgcaaca cttaccgtcc cggccatgcg gatttcacgt
     6241 atcacaagaa atacggagtc gcacctcagc ccggcggcgg acgctcgtca ggcagagaaa
     6301 cgctcgcaag ggtggcagcc ggtacgatag cgcggctgat actaggtgag caggtaaaaa
     6361 tattcagcta ttgcacagct atcagcggcg ttgtcgccaa ggtaacggac ataccgttcg
     6421 ccgaaatgca tccgctgcga ttcgctgacc ctgccctcgc cgccaaagcc gaacaggctg
     6481 ttctggcggc aaataaagaa ggtaactcag tcggcggaat aatagaattg ctcatttgcg
     6541 gcgtgccggc tggccttggc gagccggttt ttgaaaaact cgacgccgca ctggcaggcg
     6601 ccgtgatgtc cataggtgca gtcaaaggca tagaattcgg cgctggtttc agatttccag
     6661 aaatgaccgg acagcaggca aatgaccaga tgtcgccgga cgggtttgaa tccaacaacg
     6721 ccggcggtat tatcggcgga atatccacag gccagaatat aatcatgcgg ctggcggtaa
     6781 aacctacgcc gtccatcgcc gtcccacaaa aaagcataac gtttgacggc aaagccgcca
     6841 ccgtaaccac aaccggcaga catgaccctt gcgtcgcacc gcgagttgca atagtcgccg
     6901 aagcaatggc ggcaatagtg ctggctgacg ctctactctg ccagaaaacc agaattttgt
     6961 agaacccatc accaaaaaca tggacgtcgt cgaaatctgg caatgttgag gttgtaataa
     7021 ccattcaaac aatcaattat gcacggttac atccagccgc gccattacgc aatttatcaa
     7081 aagtgtaaaa aaataagttc ttccctttac acgatttatg ccctgacgac ctaaagaatc
     7141 tgtctgtttc cgcaaattcc acggctagag catgttaaac ttgagatgcg acactttatg
     7201 cgttggcgaa acgagctatg caaggaaggc gaagcaggaa tactgcccgt attccgatgc
     7261 agcctgacgc agcagagcga tgttgcagcc agcagaaatg acgcatatca agtttaaaat
     7321 gctctaagcc cgtggttttc tgtaaagaat gaataaaaaa cttattttta tcgatatttc
     7381 aggccgatat ataggataag taaattatca aaactgcccg attagatgac ttttagtata
     7441 ttatgcgaaa aagtaaagaa taattctgca agaagagaca ccttagggac ttcacgcagc
     7501 aacatagtaa aagccggtta tcatcatttt cgccggctga ccgattaagg agataggaca
     7561 tgaaacatgg ctggttaaca tgtttagcgc tagccggcgt tgcgatgctg gtcggcggtt
     7621 gcgatatgga tttcagcaaa gtcgataatc ccgaatatgc cgcctggaaa ggctttccgg
     7681 tcggcagcgg atatgaattt gaaggaatgc tccaaacttc agcggaaaat ccaaaacgaa
     7741 tacagattag cgccaacctg atggtattcg acctgactca ggctgtattg aaacgtcgcg
     7801 cgcagatttt caatcctggc ggttcaggcc agcttgagtt taccgacgta atcatcccgg
     7861 cgcgcgtaaa gaaaaacgac tgcctgctga cgcaggacgg catgcaggat aatttccgag
     7921 ggtgggattc cgtgctggtc ggagaaaaac tttacgactg tgaagttcat tattacgtcg
     7981 tcaaacgtga cggcgaaata ctgggcataa aaacgcacgg cgaaaccggc ataaaaatat
     8041 ggcaatgcaa aaaaatcccg ggcggcatgg caaaaatcga agtgacgggt ctttcgccgt
     8101 cttgcatatt ttcactttcc ggccagattg tgaacgtcgg catggcacgc atcacaatat
     8161 ctccgccagg acaagccacg gaaaccgaaa cacacccggc gaaacctgca aacaaccccg
     8221 caggaacgga cctggaaaag gctatcactt cgcctgaaca tctgccgata atgccgcagc
     8281 cggaaaaaaa agttgaaacg tcaggattga atacctcttc agctacgaca gaggaagacg
     8341 actctgcacc aaaggtaata ctgcctgcaa caaatccgtc cgataaaaaa gcattcgaag
     8401 aagttcctca aaaaattgag ccggtggaaa aaccggtcca acgacaacct gacccgcaac
     8461 ttttgagcga agttcaggaa gaagactcgc cgacaagcga accgtcttcc atcaagccgg
     8521 taaaacttga tgtgcctgaa agacttgacg aagacacggc gcccttgccg aaattcgcgg
     8581 caccggatga tagcaaatag gccagcagga atacgattat ctgtcctgtc aattttttgg
     8641 cgtttttttt tagaaaatca taacattcaa aacacggaaa atttaatatg aaaatacgcg
     8701 taataaatgg cccgaatctg aatatgctcg gcacacgtga gccggaaatt tatgggaaaa
     8761 ccacgctcgc cgatattgaa aaactgttgc gcacgacagc ggaagaatgc gaagtggaac
     8821 ttgaattcgt ccagtcgaat cacgaaggcg aaatcgtgac tgccatccag caatcgcgca
     8881 gcgcggatgg aatcatcctc aatgccgcgg cctacacaca tacgagcgta gccatacgcg
     8941 acgccatatc cgccgtcgcc gtcccgacgg tggaaataca tctcagcaac atccatgccc
     9001 gtgaagattt ccgtcaccat tcgctcatag cgccggtctg caaaggcgta atcgccggat
     9061 tcggagtgga ttcata
//
LOCUS       DAONFI010000055         8962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8921_length_8962_cov_4.593129, whole genome shotgun sequence.
ACCESSION   DAONFI010000055 DAONFI010000000
VERSION     DAONFI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8921_length_8962_cov_4.593129"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLK08_02845"
     CDS             <1..270
                     /locus_tag="PLK08_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPS52467.1"
                     /translation="VFINDNEAGLHSDYEKWLEKLAPEKPHSQYAHNGYEDNADAHMK
                     RQIMGREVVVAITNGKLHLGPWEHIFYYEFDGMRRKRLLVKIIGE"
     gene            273..668
                     /locus_tag="PLK08_02850"
     CDS             273..668
                     /locus_tag="PLK08_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52468.1"
                     /translation="MADEEDIVIPLAPLDEAAETRRKEEIRRCLEIQESLIAEQTGQG
                     VIPMEHRENLSARDMDHLIVNYCIDSYNCNIGRLRVHIEKMRPYKDIAILSANDFLYG
                     RVAEKSLNVMLPEELADYLNDLIGRLKAL"
     gene            696..1481
                     /locus_tag="PLK08_02855"
     CDS             696..1481
                     /locus_tag="PLK08_02855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPS52469.1"
                     /translation="MIGKSVAWFFSTSRDLIARRLVRWGVTPNMLTLTGCAFTSLTGV
                     MLGMAVASGQNFFYAFAVLMLYWSAACDMLDGAVARIGGKSSTFGAFLDSTTDRVSDF
                     ILWAALACGFMCRQTPNITFTLLCMTAIFNSMMISYTKSRAETLIGECNVGFWQRGER
                     FAAILFATLSANPGAAVLQQGISPAFTAWRRISYTYAVMSGGKPVKNPKDGKWYQKIQ
                     LWMYPRMTWPYDIAVLGNIAFLLFFRIDPDKMDVIRWVIFRTH"
     gene            complement(1493..2527)
                     /locus_tag="PLK08_02860"
     CDS             complement(1493..2527)
                     /locus_tag="PLK08_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPS52470.1"
                     /translation="MKCKTRTPEIPAVYTGPGLVELQVNGYAGVNFNALPENYSIDNF
                     RKAMHAMKKRGIVMALPTIITDDIDRTLLRFKLFSRYLAADAELAAFYPGVHLEGPFI
                     SREEGPRGAHNMRFCANPCDYPDIIYRFNEAASGRVVIITLAPELPGAINLIKNGVQA
                     GIIMSLGHCNPSTDDVDAAIDAGASMCTHLGNGSHQVVPRLNNYINKQLSADELAAGF
                     IADGHHIPFFALKNFLRAKGLDKSYLTTDAIAAAEMGCGVFQLGPNDKVEVTAEGRCQ
                     IPGQANLAGSALTLDSAILNVVKNCGITWEDAWKMASVNPANFAHLPTPHAVTVKIDV
                     AANHFELAKM"
     gene            complement(2711..4042)
                     /locus_tag="PLK08_02865"
     CDS             complement(2711..4042)
                     /locus_tag="PLK08_02865"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HPS52471.1"
                     /translation="MAASSIQDAISFADETKELVIRRGAISDVSAIFDKYFSGRTAVP
                     VADENTFVAAGKKVADVLKRGGIKLSEPFIFPAADLHAEDSQIDKLLARIEHTREIPI
                     AIGSGTINDMTKLASYRAGRPYICVATAASMDGYASTGAAVVVDGIKQTCPCPAPMAV
                     IADLDVICKAPSELAAYGYGDLASKITAGADWILADALGLAPIKKTAWDMVQTKLRKW
                     LANPDGVKSGKFEAIEGLFEGLVVSGLAMQYAKDSRPASGAEHLFSHIWDMKHHKFNG
                     KSVSHGFQVAIGTLATTSMYETMFNCDIQKIDIAGALAKWPSSWDDFEKFLQKQYPPK
                     DCVLRIALVQVCQKYLTPDRLAGRLENLKSQWPALQTKLANQLMPSGELAQMFSRAGC
                     PVKASQIGVNLNLLRNTMSDAANLRSRYTVLDITRELAISEICRKNFATMA"
     gene            4259..5791
                     /locus_tag="PLK08_02870"
     CDS             4259..5791
                     /locus_tag="PLK08_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPS52472.1"
                     /translation="MLKFEAYRNGKPVRGIKLAGAYMFGQDAVPVHADLLAEGHVITC
                     RMNAPETCGLALLFDAGAAGAYLLSTTRLPERSGPYLLNLELARARMRELYSKRADWA
                     LFDFEDAAELNGEFDELRKKFAHALGTAAVDFAEASLLADEVLSRAIVLGEKYALFHA
                     EILSKQRIEVSSSGLPVGCRVNMSASNNGLYVAAMKTFAGFLSVPMNWREMVPSEGDY
                     NFKAIDAWMNFAARENKTLHAGPIIDFSDDSLPEWLALYQHDYAAVKHIMIEHIRKVV
                     PRYHSYIRVWNVISGLNSKNTLDMNFEQISELTRSCCQCIRQIAPDAEIVVELTCPWS
                     EFYARNFHTIPTLLYADVIGRSDVPFDAFGLRLLMGVDVDGYYVRDLMQISSLMDEFV
                     WQGKKIHITACGVPSSADPDEQNYWGAECSVKKAGHWHGLWTRRLQAEWLHALCRLAM
                     SKSFVKSICWQDMVDISGKILPNGGLRAGDFKPKLALQELYSLQGLWKDIAARPETPD
                     GG"
     gene            5781..6149
                     /locus_tag="PLK08_02875"
     CDS             5781..6149
                     /locus_tag="PLK08_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPS52473.1"
                     /translation="MADRHVRFDIELDATQLRIIASLAVVLLTASYFSATSGYSSVPP
                     VNPKKISLAREYINPNTDSYASLRRLPGVGDLKSKAIITYRQACENPPAFRTSEDLMK
                     VNGISSGIFREAKPLIRVEY"
     gene            complement(6306..8021)
                     /locus_tag="PLK08_02880"
     CDS             complement(6306..8021)
                     /locus_tag="PLK08_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52474.1"
                     /translation="MMRPAPSPKHIPSIIILIAVSLLAGYLSWHDDSITDDQIQLLTS
                     LKANNANFAGDGIYDHHIVGGQFHHMHSIAFAGLIRAVNRICPANASPEMPMRLLVGP
                     ITLLFLLGMYALLWRQTMSSATSTFVAVLSTTMMSTFDGWHWGVGAISSITPEGLVVA
                     ISPLLMLAYLKYFNDRRIVLIFMLMGLCGNIDIVSSINMALVAMLTFLACHRFNRQAM
                     AVGVSGVFLFMIGALPNISHLLMVHAQIEQAVGQVAPIDFATVMRAFDMSYYEVTYPE
                     LLMPLWQWGLYPLTLAAVSLVVLWRLDKFRSQFIDMWVWMSISSLIVMLGFHALATAA
                     AYLTRQPLPFYVFLQASCWLMLPLYVLFARALTQVFRILEKKYRHLLRWVMAGVIVGW
                     MLPSDALRPARYGLYRLAAMTPIGQNSLKINDIMERKAKRQELVNIARWQNKNCVNSF
                     VYIDDPKYRLNSGQAILTCREDFIYYYLLLPSQLQQWMDNLHETRKILARPLNIQELT
                     NDAIKRVSVIPQISQVYIITSDKEIADNPQNLQEIKSDNWGKYWKIYRIPLSNPNPKI
                     SEYRA"
     gene            8192..>8962
                     /gene="truD"
                     /locus_tag="PLK08_02885"
     CDS             8192..>8962
                     /gene="truD"
                     /locus_tag="PLK08_02885"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HPS52475.1"
                     /translation="MHEDIFAGWPCLTRDFAGIGGEIKVHPDDFRVDEIPAYMPCGEG
                     THVFVRAEKRGMTTPAATGRLAKILGINPADVGYAGLKDARAVTTQYFSMEHVDEKKL
                     CGYDDGQLKVVSISRHGNKLKTGHLLGNRFVIRIRGVSREQLGNARAIFDVLCRRGVP
                     NYFGVQRFGARRDTHLLGEALVRDDRDGFLKKFLGSPQAGDFCDMQAARDSFDAGCLD
                     RALKLWPRQFEDQRKALLMFRKTGRPGLAIGAVDKRMKR"
BASE COUNT         2104 a         2117 c         2438 g         2303 t
ORIGIN      
        1 gttttcatta atgacaacga agccggcttg cacagtgact acgaaaaatg gttggagaaa
       61 ctcgctccgg aaaagccgca cagtcaatac gcccacaacg gctatgagga taacgccgac
      121 gcgcacatga aacggcagat tatgggccgt gaagtcgttg tggcgattac gaacgggaaa
      181 ttgcacctcg gcccgtggga acatattttt tattacgaat tcgacggtat gcgtcgaaag
      241 cggctgcttg tgaaaataat cggagaatga gcatggctga tgaggaagat atcgtcatac
      301 cgctggcgcc gctggatgag gcggctgaaa cacgtcgcaa agaagaaatc agacgctgcc
      361 ttgaaatcca ggaatcgctg attgccgagc agaccggcca gggcgtcatt cctatggaac
      421 atcgcgaaaa tctatccgca cgcgatatgg atcacttaat cgtcaattat tgcattgata
      481 gttataattg taatattggc aggctgagag tccatattga aaaaatgcgg ccgtataaag
      541 atattgctat cctgtcggca aatgatttcc tctacggcag agtggctgaa aagtcactca
      601 atgtaatgct gcctgaggaa cttgccgatt atctgaatga tcttattggt cgattaaagg
      661 cattgtaaaa ctttttgatg gaatgggaat tttgaatgat aggcaaatct gttgcatggt
      721 tttttagtac gtcgcgcgat ttgatagcca gacggcttgt gcggtggggc gttacgccga
      781 acatgttgac gttgacgggt tgtgctttta cgtcgctgac gggtgtaatg ttgggcatgg
      841 cggtggctag cggccagaat ttcttttacg catttgccgt gctgatgctt tattggagtg
      901 cggcttgcga catgcttgac ggcgcggtgg ccagaatagg cggcaaatcc agcacgttcg
      961 gtgcatttct tgacagtact accgaccgtg tcagcgattt tatcctttgg gctgcgttag
     1021 cctgcggttt tatgtgccga caaacgccta atataacttt tacactgttg tgtatgacgg
     1081 cgattttcaa cagtatgatg atatcgtata cgaaatcccg cgccgagacg ttgattggcg
     1141 aatgcaatgt cggattctgg cagcgcggcg aacggtttgc ggcgatactt tttgcgacgt
     1201 tgtcggctaa tccaggcgcg gcagttttgc agcagggcat cagtccggcg tttacggcgt
     1261 ggcggcggat aagttataca tatgcagtta tgtccggcgg aaaacctgta aaaaatccaa
     1321 aagacggaaa gtggtatcag aagatacagt tgtggatgta tcccagaatg acatggcctt
     1381 atgacattgc ggtactggga aacatagcat ttttgctgtt tttccgaatc gaccctgaca
     1441 aaatggatgt aatccgctgg gttatttttc gaacccatta gttctgatgg gcttacattt
     1501 tcgccaattc aaaatggttt gcggcaacgt cgatttttac ggtaacggcg tgcggcgtcg
     1561 gcaaatgtgc gaaattcgca gggtttacgg aagccatctt ccacgcgtct tcccatgtta
     1621 ttccgcagtt tttcacgacg ttcagtatgg ctgagtcgag cgtcagggct gagccggcca
     1681 ggttggcttg gccgggtatc tgacatctcc cttcggctgt cacttcaact ttatcatttg
     1741 ggccgagctg gaatacgccg cagcccattt ctgctgcggc tattgcgtcg gtggtgagat
     1801 aacttttgtc gagccccttg gcgcgtaaaa aattcttcaa cgcaaagaat gggatgtgat
     1861 ggccgtccgc aatgaaaccg gcagcaagtt cgtcggcact gagctgcttg ttgatgtaat
     1921 tgttcagccg cggaactacc tgatgtgacc cattgcccag atgcgtacac attgatgctc
     1981 cagcgtcgat ggctgcatcg acgtcatccg tcgacgggtt gcaatgtccc agcgacatga
     2041 ttatgcctgc ctgtacgccg tttttgatga gatttatagc gcccggcaat tccggagcaa
     2101 gcgtgatgat tactacccgg ccgctggctg cttcgttgaa tcgatatatg atgtcgggat
     2161 agtcgcaagg gtttgcacag aaccgcatgt tgtgcgcgcc gcgagggcct tcctcccgcg
     2221 agataaacgg gccttcaagg tgaacgccgg ggtaaaatgc cgcaagttcg gcatcggcgg
     2281 caagatatcg ggaaaaaagc ttgaatcgca gcagcgtacg gtcaatgtcg tccgtaatta
     2341 tcgtcggcag cgccatcacg atgccgcgtt ttttcatcgc gtgcatcgcc ttgcggaaat
     2401 tgtctatgga ataattttcg ggcagagcgt tgaaattgac gcctgcgtaa ccgttgacct
     2461 gaagctcaac aaggccgggc cctgtataaa cggcgggaat ttccggcgtc ctggttttgc
     2521 acttcatgat acccttcctg ttttttaaac tggcgatata agacgttatt gtaaatcctg
     2581 aaatattgat aaatacaaaa cgtatgatac atccgccacg taaaccgtta cactcctacc
     2641 cattgaatga tatgtcattc aaattgaatt atccaccata aattaaacct ttgtcagcgg
     2701 agcattatta ttatgccatt gttgcaaaat ttttacggca tatttctgaa atcgccagtt
     2761 ccctcgtgat atccaggacg gtgtatctgc ttctgaggtt ggcagcgtct gacatggtgt
     2821 tcctgagcag gttgagattt acgccgattt gtgatgcctt taccggacag ccggcgcggc
     2881 tgaacatctg ggcgagttca ccggagggca tcagttggtt agccagtttc gtctgaagag
     2941 ccggccattg ggatttgagg ttttccagtc ggccggccag cctgtcaggc gtcaggtatt
     3001 tttggcagac ctgcactagg gctatccgca gtacgcagtc ctttggcgga tactgttttt
     3061 gcaaaaattt ttcgaagtcg tcccagcttg acggccattt agccaacgca ccggcgatgt
     3121 cgattttctg aatgtcgcag ttgaacatcg tttcgtacat ggatgtcgta gccagcgttc
     3181 ctatggccac ctgaaagccg tgcgatacgc ttttgccgtt gaacttgtga tgcttcatat
     3241 cccatatatg gctgaacaga tgttccgctc cactggcggg gcgcgagtct ttggcgtatt
     3301 gcattgccag gcccgaaacc acaaggcctt cgaaaaggcc ttcgattgct tcaaacttgc
     3361 cagatttcac tccgtctgga ttagccagcc attttcgaag tttcgtctgc accatatccc
     3421 acgccgtttt ttttatgggt gccaggccga gcgcgtcagc cagaatccag tctgcgccgg
     3481 cggtgatttt cgatgccagg tcgccgtagc cgtatgccgc caattccgat ggagctttgc
     3541 atattacgtc aaggtcggcg atgacagcca tcggagcggg acatggacat gtctgcttta
     3601 tgccgtcgac taccaccgcg gcgccggtcg atgcgtaacc gtccattgaa gcggctgtgg
     3661 cgacgcatat gtatggccgg cctgcgcggt aactcgccag cttagtcatg tcgttaatcg
     3721 tgcccgaacc gattgctatg ggaatttcgc gggtatgttc aattctcgca agcagtttgt
     3781 cgatttgcga atcttcggcg tggaggtcgg cggctgggaa tatgaacggc tcggataatt
     3841 ttatgccgcc gcgttttaga acgtccgcga cttttttgcc ggctgcgacg aaggtgtttt
     3901 cgtcggctac cggcacggcg gttctgccgc tgaaatattt gtcgaaaatt gcggatacgt
     3961 ctgaaatcgc accgcgacgg atgacgagtt cctttgtctc atctgcgaaa gaaattgcat
     4021 cctgaatact gctggcagcc attttgtctt atacctcgat tgcacgcaga gttgttttgt
     4081 ctgactgcga catttgtcgg aatccgtcag cagggacgga aataaaaccg cgtacttcat
     4141 gaatatatag ctctgccgcc ggttcatcca gaaaaaattg attctggcgt ttaaaatctt
     4201 tcgattgtcg gcggttaacg tcttgaggct gtcggctggt gttgatatga tgtagccgat
     4261 gttaaagttc gaggcatata gaaacggcaa acctgtgcga ggaatcaagc tggctggtgc
     4321 gtatatgttc ggccaggatg cggttccggt tcatgccgat ctgcttgccg aaggtcatgt
     4381 cattacgtgt cgtatgaatg cgccggagac gtgcggcctt gcgctgctgt ttgatgcagg
     4441 tgcggcaggc gcgtatcttc tttcgaccac ccgtctgccg gagagaagcg ggccgtatct
     4501 tctcaacctg gaactggctc gcgcacgtat gcgtgaactt tactccaagc gtgccgactg
     4561 ggcgctgttt gattttgagg atgccgccga actcaacggc gagtttgacg agctaagaaa
     4621 aaaattcgct catgcgcttg gcacggctgc cgttgatttt gccgaggcgt cgctgctggc
     4681 tgatgaagtt ctgagtcgtg ccatcgtact tggcgaaaaa tatgccctct ttcatgcgga
     4741 gattctgtca aagcagcgaa tagaggtttc ctcgtctggt ttgccggtgg gctgccgcgt
     4801 gaatatgtca gccagcaata acggcttgta tgtagccgcc atgaaaacgt ttgccggttt
     4861 tctttccgtg ccgatgaact ggcgtgaaat ggtgccgtct gaaggcgatt acaatttcaa
     4921 agccattgat gcatggatga attttgccgc cagggaaaat aaaactcttc atgccggccc
     4981 gattatagat tttagcgatg attctcttcc tgaatggctg gccctgtatc agcacgatta
     5041 tgccgccgtc aaacacatca tgatagaaca catcaggaag gttgttccgc gatatcactc
     5101 ctacattcgc gtgtggaacg tgatttccgg cctcaacagc aaaaatacgc tcgacatgaa
     5161 ttttgaacag atatcggaac tgacgcgaag ctgctgccag tgtattcgcc agattgcgcc
     5221 tgacgccgag atagtggtgg aactcacctg tccctggagc gaattttatg ccagaaattt
     5281 tcatacgatt cccactctgc tctatgccga tgtcatcggc cgcagtgatg tgccgtttga
     5341 cgctttcggc ctgcggttgc tgatgggtgt ggatgtggac ggttactatg tgcgcgatct
     5401 catgcagata agttcactga tggacgagtt cgtgtggcag ggcaaaaaaa tccacataac
     5461 agcctgcggc gtgccaagtt cagccgaccc tgatgaacag aattattggg gggctgagtg
     5521 cagcgtaaaa aaagcgggcc attggcatgg cttgtggaca aggcggttgc aggctgaatg
     5581 gctgcatgcg ctgtgccggc tggcgatgag caaatctttt gtaaaaagca tatgctggca
     5641 ggatatggtc gacatttccg gcaaaatact gcccaacggc ggtttgagag ccggcgattt
     5701 taaacccaaa ctcgcactac aggaactcta cagccttcag ggactctgga aagacatcgc
     5761 cgcgagaccg gagacgcctg atggcggata ggcatgtcag attcgatatc gaactggacg
     5821 ctacgcaact cagaataatt gcaagtctgg ctgttgtatt attgaccgcg tcgtattttt
     5881 ctgccacttc aggttattca tccgtgccgc ctgtcaatcc gaaaaaaatc tcactggccc
     5941 gtgaatatat caatcccaac accgattctt atgcttcatt acggcggctg cctggtgtag
     6001 gcgacttgaa atcgaaggct attataactt atcgtcaggc atgtgaaaat ccgcccgcgt
     6061 ttcgcacatc ggaagacctg atgaaagtta acggaatcag tagcggaatt tttcgcgaag
     6121 caaaaccgct aatcagagtt gaatactaga gcattgaaag attgagagtt catatttttg
     6181 cgccagtggg tttgttcgtg gcaaggaagg aaaatgaggc atacttggca gtattctgaa
     6241 gtttttctga cgcagccacg gacaaagcaa accagcaaaa aaggaactct caatctttca
     6301 atgctctagg ctcgatattc cgaaattttc ggattgggat tggacagcgg aatgcggtaa
     6361 attttccagt atttgcccca gttgtcggat ttgatttcct ggagattttg aggattgtcg
     6421 gctatttcct tgtccgacgt tatgatgtag acctgtgata tttgcggtat tacgcttacc
     6481 cgttttatgg cgtcgttagt gagttcctgg atgttcagcg gccgggccag gatttttctg
     6541 gtttcatgga gattgtccat ccactgctga agctgcgacg gcaatagaag ataataatat
     6601 atgaaatctt cacggcaggt cagaatggct tggccggaat tcagacggta tttggggtcg
     6661 tcaatataaa cgaatgaatt gacgcagttt ttgttctgcc atcgtgctat attgaccaat
     6721 tcctgtcgct tggcctttcg ttccatgata tcgtttattt tcagactgtt ttggcctatc
     6781 ggcgtcattg ccgccaggcg atacaggccg tagcgtgccg gccgtaatgc gtccgacggc
     6841 agcatccagc cgacaatcac gccggccatg acccagcgaa gaagatgtcg atattttttt
     6901 tccagtatac ggaatacctg tgtaagtgcg cgggcaaata gtacgtacag cggcagcatc
     6961 agccagcatg acgcctggag aaatacgtag aacggcaacg gctggcgagt cagatacgcc
     7021 gccgccgtcg ccagtgcatg aaagcccagc atcacgatga gcgacgaaat cgacatccat
     7081 acccacatgt cgatgaattg cgaccggaac ttgtccagcc gccacagtac caccagtgaa
     7141 acggcagcta aagtcagcgg gtaaagcccc cactgccaca gcggcatgag caattcggga
     7201 tacgtcactt cgtaataact catgtcgaag gcccgcatta ccgttgcgaa atcaatcggc
     7261 gctacctgtc cgacagcctg ctcaatctgt gcgtgaacca ttagaagatg cgatatgttc
     7321 ggcagcgcac ctatcatgaa aagaaaaacg cctgaaacgc ctacagccat tgcctgccga
     7381 ttgaacctgt gacaggccag aaacgtcagc atcgccacca gcgccatgtt tattgatgaa
     7441 acgatatcga tattgccgca cagccccatg agcatgaaaa tcagcacaat gcgtctgtcg
     7501 ttaaaatatt tcagatatgc cagcatcaac agcggcgaaa ttgccactac caggccttcc
     7561 ggcgttattg aagaaattgc gcctacgccc caatgccagc cgtcgaacgt actcatcatc
     7621 gtcgtgctta acacggcgac gaacgtggag gtggcactgc tcatagtctg ccgccacagc
     7681 agggcgtaca tgcccaacag gaacagcagg gtgattggcc cgacaagaag gcgcatgggc
     7741 atttcagggc tggcatttgc cggacagatg cgattgacgg ctcgtataag gccggcgaac
     7801 gctatcgagt gcatgtgatg gaattggccg ccgactatgt gatggtcata aatgccgtcg
     7861 ccggcgaagt tggcgttgtt tgctttcagc gaggtaagca gctgaatctg gtcgtcggtt
     7921 atggaatcgt catgccatga cagatagccg gccaacagcg atacggctat caatattatt
     7981 atagacggta tatgttttgg agacggtgca ggtctcatca tgcattcctg ttcaaatctg
     8041 gcggcatatt ttgtaaaaag tgcattttgt ttttctaaaa aaaactccca gctgtgacgg
     8101 ctcgtgaaaa atatatatca aggggattaa aaatgcagca tacttatagt atattcttgt
     8161 gtcttggcaa cttgcaacag gaattacaaa catgcatgaa gatatatttg ccggctggcc
     8221 ctgcctgacg cgggattttg ccggcattgg cggcgaaata aaggttcatc cggatgattt
     8281 tcgcgtagac gaaatacctg cgtatatgcc gtgtggcgaa gggacgcacg tattcgttcg
     8341 tgcagaaaag cgcggcatga cgacacctgc ggcgacaggg cggctggcga agattttggg
     8401 catcaatccg gcggatgtcg gttacgccgg ccttaaggat gcccgcgcag tgaccacaca
     8461 gtatttcagc atggagcacg tggatgaaaa aaaactctgc ggttacgacg acggccagct
     8521 gaaggtcgtc tcgatttcca ggcatggcaa caaactcaaa accggccatc tgctgggcaa
     8581 caggttcgtc atacgcattc gcggcgtcag ccgtgagcag ttgggaaatg cccgcgcgat
     8641 tttcgacgtg ctttgtcgtc gtggcgtgcc gaattatttc ggcgtacagc gattcggcgc
     8701 acgcagggat acgcatcttc tgggcgaggc gcttgtgcgt gacgatcgtg acgggttttt
     8761 aaaaaaattt ctgggtagtc cgcaggctgg cgatttttgc gacatgcagg ctgcgcggga
     8821 ttcttttgat gcgggctgtc ttgacagggc gttgaagctc tggccgaggc agtttgagga
     8881 ccagcgaaag gctttgttga tgtttcgcaa gactggccgg ccggggctgg ctatcggggc
     8941 tgtggacaaa cggatgaaaa ga
//
LOCUS       DAONFI010000056         8910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_8995_length_8910_cov_3.425748, whole genome shotgun sequence.
ACCESSION   DAONFI010000056 DAONFI010000000
VERSION     DAONFI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8995_length_8910_cov_3.425748"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLK08_02890"
     CDS             <1..809
                     /locus_tag="PLK08_02890"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008531 - riboflavin kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HPS52476.1"
                     /translation="AGGPLTAMTFDPPPDIIIRPADAPQRICTPDDRAALLLEAGCDY
                     VVTAKTDLQLLRMSPEDFIARIIVGRFNPRYIVEGPNFFFGHFRAGNVETLKNAGKKY
                     GFQTQVVEPVTVELAGVRARVSSTLIRQLIEEARIEDADRLLTRPFSFVGQVIGGEKV
                     GRVLQYPTANIDPGMQVIPCDGVYAGLATVHGKPYRAAISIGRKPTIGDNLPRLVEAN
                     LLDASGDFYHEKMRLRFIRFLREQRKFDSLDELKAQISRDVRQTREIVTL"
     gene            complement(983..2818)
                     /locus_tag="PLK08_02895"
     CDS             complement(983..2818)
                     /locus_tag="PLK08_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52477.1"
                     /translation="MTHMNDTKDGRFPLRGYSPPLLKPRGPIRVILATLVNLLLFTVA
                     AGFFGYLSSGKPWDFSSAAYRKDLLTPFAEFLLEPVGIFSQPWMILVYGVLLGILIFV
                     PVAVSVLFQLLLSMVFVLIVGGMGHSWIFALSLAIGCVIAARTRLRRSWPFGAMLLGM
                     TPSVLFLYFLAFPSLDSAVLLPIQRWILILCLTLGVVIAAVFCGICVALVRWRKFQPG
                     VIWPMLLGGLLTGFVIFWVKVGPAEVDYALLTSGLADSQSIFPAQSAERWQKERHKTP
                     SKPYILLILENLESRKAALIRRCNGFLNRYPDSRRAASVAFLRAQLECVQLDLSIFDE
                     RFACYTAEFVNPDSYSAWLYLLEKYPTSDSAAMAYYNIGILKLRQAAGKANLEIRLNL
                     AHDANGMLWQAQDRLRDIVVRLRHDTDEKGIFQPLPDMPDFQTYENALFDVEKLTWML
                     NDNDILSNPKSTLAMARLLSINPCSPNYVREVKRLAADESFRDTPIWDNLQLAVAKLN
                     PNPYDRAEAIARISAKPHSDAAIEANYELGCLSMQKANASFIRLVLKEPEYYFTVVVS
                     ERFNPWLAKAARQLEWLHSNVEQRLPQSAEPSTHPSTQPSTMPAS"
     gene            complement(2824..3456)
                     /gene="rdgB"
                     /locus_tag="PLK08_02900"
     CDS             complement(2824..3456)
                     /gene="rdgB"
                     /locus_tag="PLK08_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006789729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPS52478.1"
                     /translation="MAVTITLGSGNPHKLKEVSAVLSALGVAVLPLPADRKIPEPYET
                     GSTFAENARQKALYYADILDCWCLADDSGLVVDALDGLPGVISARYAADCCPANSSRK
                     VIDCANNAKLLAVLTSVPDDRRTARFVCHLALAAPGKILLESSGKIEGRILHAPAGEN
                     GFGYDPLFYVDEKGCTTAQLSSHDKNAISHRGCALRTFAKKLRILLAEME"
     gene            complement(3425..4387)
                     /locus_tag="PLK08_02905"
     CDS             complement(3425..4387)
                     /locus_tag="PLK08_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52479.1"
                     /translation="MLKQFGIILIFALLLIGCDDNFAPKTSGSGDVSSGGGGWNLGGQ
                     QKFAHDAEPQTILLQLFEGPDHVDRAVSQKNYLADTLQWHDVYVVHEDDHSILYMGSY
                     GSDGASQRQIQANLAKAKNFRMANGVQPFVKAIVIAKPGTADEGPSEWNLKNAKGYYS
                     VLVDIQYDVPQSGYYGRKQRAVMDCRNLRKQGYDAYYFHGPYKSGVAVGTFPETSIKT
                     VMVKARHPITHNEFEVEKKVIVDPKMKKVFQDFPEMLICGNTEKTVEIYYENGRPKRH
                     AVTQEPYPIIIPNAENKKTKEKEKQSDAGNRSTPGGGNNNIRLW"
     gene            complement(4428..5147)
                     /gene="rph"
                     /locus_tag="PLK08_02910"
     CDS             complement(4428..5147)
                     /gene="rph"
                     /locus_tag="PLK08_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPS52480.1"
                     /translation="MSNKRIDSRRAGQLRPVTITPDFVRTADGSCLIEMGGTRVICTA
                     SIENTLPKWRDADGGGWVTAEYGMLPASTGSRKSRPTLKPDSRSIEIQRLIGRSLRSI
                     VRFDRLGPNSITLDCDVLEADGGTRTASITGAYVALQRAVEKGIAKGFFKKGAIRGAV
                     AAVSVGIINGKALLDLCYAEDSIADVDMNVVMTSSGKFVEIQGTSEAAPFTAVQLQQM
                     LALAGKGIKELLHVQKQALAR"
     gene            complement(5164..5721)
                     /locus_tag="PLK08_02915"
     CDS             complement(5164..5721)
                     /locus_tag="PLK08_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52481.1"
                     /translation="MKPLIHGITHNPRIDVLAVLRRLFAGPASGADVATFLDENFVPP
                     AEVDVDTLVLKTRSGEWCVGLDDIAKIVGGKWEFIDGIDAGFAREVVSADGDTAWLDC
                     EAEFLVKFRHGGFLGLWAKKIAETVKNPDGLILADIIERAEKIDTGLFEQDCGAKYAI
                     FCRVSGILSRKGHPWRFQQIRLSFR"
     gene            5818..8826
                     /locus_tag="PLK08_02920"
     CDS             5818..8826
                     /locus_tag="PLK08_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HPS52482.1"
                     /translation="MKRRSAIIFILSFLWVCYYGQATGDGRPLSGGEKIYRIGVQMDA
                     SRDICLKKWKPLAEYLSRRIYGCSFRIIPLKIGVIPYAIENGDVDFILCNPTFYAIFC
                     QQRNVTQLATRINNDTGRPLSRYAGTIFCRTDNESIYCINDIKGRIFACPNAYSLGGW
                     LSVWREMLKQDFNPQTDTKELKTFATQEEVVKAVLNGTAEAGSVRSDVLELMANSKQI
                     HPEDFRIINRKMDDFALPHSTECYPEWGFVQLQHVPNGIAKQVTVALLQMTPRDYAAV
                     AAEIAGWTSPENCQPVIECLEYLNLSPYIKTKTEGWLVVVDRYWPILLGGGIIILTSM
                     IVAVLAIRANMKYRDLKHELSQRQDRMSEIFDCSDEGLAYCNNNDEIIYVNQTFCRLT
                     GCTESELTGDNIFHRLPTDLCHSASRLIDEYRNNNYSNPHEMTYCINGIDVAMKIKPF
                     YADEKYHGALFSLADINDLMTARREIAAINHELEDSIRRANRLAMRALQADQAKANAI
                     TNLNRDIRTPLNGIMGLANILENTELQPQQKNYIDILKNSIDTMVTLLDKALDMSRLE
                     FEQISLDRVLFDVRDEISQILKLLRFQAAEKGLSLDFNVENDVPQKICNSPDRLRQIL
                     INLVSNSIHHTEKGKISLRVTSGRTVNDGCEIKFAVSDTGIGISPEKLETIFDKIPAG
                     DNLLDVHGGTGLALAISSKIARLMGGSLVAESSPGIGSTFTLTIVDFHSSGTARETRT
                     PDTSHSENEGVLEGLKVLIAEDNQVNRLVLDEILLKHGCTVTCVENGRAAVEEVVNDH
                     FDLVLMDCHMPVLDGFEATLEIRRLAPPFRNIPILAITANALPQIKDYCFRCGMNGYI
                     SKPFSAETLKTAIIRCLSGAPAAANERAANTAQNEETIDVDQQALVERIGKSGDVMKH
                     VVELFLDDCVKSIAQLHKCFDENDMTQAAMVSHSLKGSSALAGAARIQNAAMEAEQAA
                     LDANREYLGKVIEIIEECFKKYKTAVSACI"
BASE COUNT         2301 a         2436 c         2200 g         1973 t
ORIGIN      
        1 aggctggcgg cccgctgacg gcgatgacct tcgacccgcc gccggatata ataatccggc
       61 cagccgacgc gccgcaacga atctgtacgc ctgacgaccg tgccgccctg ctcctggaag
      121 ccggctgcga ttacgtagtg actgcaaaaa cggatttgca actgctgcga atgtcgccgg
      181 aggattttat cgcccgaata atcgtcggcc ggttcaaccc gcgttacatc gttgaagggc
      241 ctaatttctt cttcggccat tttcgagccg gcaatgtgga aacgctcaaa aacgcgggca
      301 aaaagtacgg ctttcagacg caagtagtcg aacctgtgac tgtcgagctc gccggcgtgc
      361 gtgcgagagt ttccagcacg ctcatccgac agttaatcga agaagcccgc atcgaggacg
      421 cagacaggct gcttacccga ccattttcat tcgtcggcca ggttatcggc ggagaaaaag
      481 tcggccgtgt gttgcagtac ccaaccgcca atatcgaccc cggcatgcag gtaatcccat
      541 gcgacggcgt ctacgcaggc cttgcaaccg tacacggcaa accataccgc gcagctatca
      601 gcataggccg aaaaccgacc atcggcgata atctgccgcg gctggtggaa gcaaatctgc
      661 tggatgcgtc gggtgatttt taccatgaaa aaatgcggct gagattcatc cgctttttgc
      721 gggaacagcg aaaattcgac tcgctggatg aactcaaagc ccagatttcc cgtgatgtgc
      781 gacagactcg cgaaatagtt acgctatagc gttgcctgaa agttttgcaa tgttctaatg
      841 tacgggcctt tattaacagg gtttgttgca agttttcata ttgcgttatt ttcaaaaacc
      901 gctatataaa atccattcca aaaacctgac tgcctgcaat ctgtaaattt tttaaaaaaa
      961 tcaggataaa atcacaaaaa cttcaactcg caggcatggt tgagggttga gtggacggat
     1021 gcgtcgaagg ctcggcggat tggggcagac gctgttccac gttcgaatgc agccattcca
     1081 actggcgggc ggcttttgcc agccagggat tgaatcgctc ggaaaccacg acagtaaaat
     1141 aatattccgg ctctttcagg acaagccgta taaacgacgc attggctttc tgcatggaaa
     1201 gacagcccag ttcgtaattg gcttctatgg cggcgtcgct gtgcggtttg gcggaaatcc
     1261 tggcaattgc ttccgcgcgg tcatacggat tgggattgag cttggcaacg gcaagctgca
     1321 gattatccca tatcggcgta tccctgaacg attcgtcggc agccagccgt ttcacttcgc
     1381 ggacataatt gggcgaacat ggattgatgg ataacagccg tgccatcgcc aacgtgcttt
     1441 tcggattcga aagtatatcg ttatcgttca gcatccaggt aagtttttca acatcaaaca
     1501 gggcattttc gtaagtctgg aagtcgggca tatccggcag cggctgaaag atgccctttt
     1561 catcggtatc atgccgcagc cttacgacga tatccctcag ccggtcctga gcctgccaaa
     1621 gcatgccgtt tgcatcatgc gcgaggttga gacgtatttc aaggttagcc ttccccgcag
     1681 cctgccggag tttcaatatg ccgatgttat aatacgccat cgcggcggaa tcgctcgtcg
     1741 ggtatttttc gagcaggtac agccatgcgg aataggaatc cggattcaca aactccgccg
     1801 tgtaacacgc aaaccgctcg tcaaatatgg acaaatccag ctggacgcac tccagctgcg
     1861 ccctcagaaa agccacagat gccgcacgtc tcgaatccgg atacctgttc agaaatccgt
     1921 tgcaacgacg aatcagcgcg gctttgcggg attcgagatt ttccagaatc agcagaatgt
     1981 acggctttga cggcgttttg tggcgttctt tctgccaccg ttccgcggac tgcgccggaa
     2041 atatgctctg gctgtccgcc aagccgctgg tcagcagtgc ataatccact tccgccggcc
     2101 ctactttgac ccagaatatg acaaaaccgg tcaaaaggcc gcccagcagc atcggccata
     2161 ttacgccggg ctggaattta cgccatcgta ccaatgctac acagatgccg cagaaaacgg
     2221 cggctatcac aacacccagc gtcaggcaga gtatcagtat ccagcgttgt atgggcagaa
     2281 gcacggcgga atccaggctg ggaaacgcga ggaaatagag gaacagcacc gacggcgtca
     2341 tgccgagaag catcgcgccg aatggccacg aacgacgcag cctggtacgg gctgcaatca
     2401 cgcaacctat cgccagcgac agtgcgaata tccagctatg gcccatcccg ccaactatca
     2461 gcacgaacac catggacagc agcaactgaa acaataccga caccgccacc ggtacgaata
     2521 tcagtatgcc gagcaatacg ccgtaaacca atatcatcca cggctgagag aatatgccca
     2581 caggttcgag taaaaattcg gcgaacggcg tcaataaatc cttacgatag gcggcagaag
     2641 aaaagtccca cggcttgccg ctggagagat agccgaaaaa acccgccgcc acagtaaaca
     2701 ataaaagatt caccaacgtg gccaatatta cccttatggg ccccctgggc ttaagcagcg
     2761 gcggcgaata gccgcgtaac ggaaaacggc cgtctttcgt atcattcata tgcgtcattc
     2821 ccgctactcc atttccgcca gcagaattcg aagtttttta gcgaaagtcc gcaacgcaca
     2881 cccgcgatgg ctgatggcgt ttttatcgtg cgacgataat tgcgcggtcg tacagccctt
     2941 ttcatcaacg taaaacagcg gatcataacc gaatccgttt tcgccggcgg gagcatgcaa
     3001 tatgcgaccc tcgattttgc cggacgattc aagcagaatt ttacctggcg ccgcaagagc
     3061 cagatggcag acgaatctgg cggttcgccg gtcgtcaggc acggatgtaa gcacagctaa
     3121 cagtttggcg ttattggcgc aatcgatgac tttacgcgag gagttcgccg gacaacagtc
     3181 ggcggcgtag cgcgcgctta tcacgccagg caagccgtca agcgcatcga ccacaaggcc
     3241 ggaatcgtcg gcaagacacc aacaatccag aatatcggca taatacaacg ccttctggcg
     3301 ggcattttct gcaaaagtgc tgcccgtttc ataaggttcg ggaatttttc tgtccgccgg
     3361 cagaggcaga acggctacgc ccaatgccga cagcactgct gaaacttcct ttagtttgtg
     3421 cggattacca gagcctaatg ttattgttac cgccacctgg ggtactcctg ttgccggcat
     3481 cggactgttt ttccttttcc ttagtttttt tattttccgc gttgggaatg attatcggat
     3541 acggttcctg ggtaacggcg tgacgcttcg gccggccgtt ctcataatat atctcaaccg
     3601 ttttttccgt attcccgcat atcagcattt ccggaaaatc ctggaaaact ttcttcatct
     3661 tcgggtctac gataaccttc ttttccactt caaactcgtt gtgcgtgata ggatgcctcg
     3721 ccttgaccat tacggttttt atcgacgttt ccggaaaagt gccaaccgct acgccggact
     3781 tgtatggccc atgaaagtaa tacgcatcat aaccctgctt gcgaagattg cgacaatcca
     3841 tgactgcccg ctgcttgcgg ccataataac cgctctgcgg cacgtcatat tgaatgtcca
     3901 caagcacact gtaatagcct ttggcgtttt tgagattcca ttcggacggc ccttcatcag
     3961 ccgtgcccgg cttggcgatt acgatagcct tcacaaacgg ctgaacgccg ttggccatgc
     4021 ggaaattctt agccttcgca agattggcct gaatctgccg ctgcgacgca ccgtcactgc
     4081 cgtagctgcc catgtaaaga atactgtggt catcctcgtg aacgacatag acgtcgtgcc
     4141 actgaagcgt atcagccaga taatttttct gcgagacggc cctgtcaaca tggtccggcc
     4201 cttcaaaaag ctgcaataat atggtctgcg gctcagcatc gtgtgcaaac ttctgctgcc
     4261 cgcccagatt ccagccgcca ccgccggacg acacatcacc actgccggaa gttttcggtg
     4321 cgaaattgtc gtcgcagcca atcagcaaca atgcgaaaat caatataatg ccaaactgtt
     4381 tcaacatctc gattctccaa aattacaaca agaaagcaac cgccagctca cctggccagt
     4441 gcctgcttct gaacgtgcaa aagctccttt atgccttttc cggcgagcgc aagcatctgc
     4501 tgcaactgca cagccgtaaa cggcgcagcc tcgcttgtgc cctgtatctc gacaaatttt
     4561 ccgctgctgg tcatcaccac gttcatgtca acgtcagcaa tagaatcttc cgcgtagcat
     4621 aaatccagaa gcgctttgcc gttaattatg ccgaccgaca cggcagccac cgcgccgcgt
     4681 atcgcacctt ttttaaaaaa acctttcgca atgccctttt cgacggcacg ctgcaacgcg
     4741 acatatgctc cggttatcga cgcagtgcga gtgccgccgt cagcctccaa tacgtcacag
     4801 tcgagcgtta tcgaattggg gcccagccgg tcaaagcgaa ctatgctgcg taatgacctg
     4861 ccgataagcc gttgtatttc aatactgcgg ctgtcgggtt tgagcgtggg ccggcttttg
     4921 cggctgccgg tactggccgg caacatgccg tattcggcag tcacccaacc gccgccgtca
     4981 gcgtcacgcc acttgggaag cgtattttct atgctggcgg tacatatcac gcgagtgccg
     5041 cccatttcga taaggcatga gccgtcggct gtcctgacga aatcgggcgt tatggtcacg
     5101 ggcctcagtt ggccggctct gcgagagtct attctcttgt tggacatctg tcgccagtct
     5161 cccttacctg aaagataatc ttatctgctg aaaacgccac ggatggcctt tccgcgaaag
     5221 tataccagaa acccgacaaa aaatcgcata ttttgccccg caatcctgtt caaaaagtcc
     5281 agtgtcgatt ttttcggctc gctcaatgat atcggcgaga ataaggccat ccggattttt
     5341 gacggtttca gctatttttt ttgcccaaag ccccaaaaat cccccatgcc gaaatttgac
     5401 aaggaactcc gcctcgcaat ccagccaggc ggtatcgccg tcggcagaga cgacttcgcg
     5461 ggcaaaaccg gcgtcaatgc cgtcgataaa ttcccatttc ccgccgacga tttttgcaat
     5521 atcgtccaag ccgacgcacc attcgccact gcgggtcttc agcaccagcg tatccacatc
     5581 gacttcagcc ggcggcacaa aattctcatc cagaaacgtc gcaacatccg ccccggaagc
     5641 cgggccggcg aaaagacgac gcaacaccgc cagtacgtct atccgcggat tgtgcgttat
     5701 tccatgaatc aacggtttca tttgcatggc ggaattataa caacttttcg acatgatttt
     5761 aagatttgat gtaattttga ttgcaaactt atataataaa gcggctggag acgatagatg
     5821 aaaaggcgtt cggcaattat tttcatcctg tcctttttgt gggtatgtta ttacgggcag
     5881 gcaacgggtg acggccggcc gttatccggg ggcgaaaaaa tatacaggat tggcgtccag
     5941 atggatgcca gcagggatat ctgtctaaaa aaatggaaac cgctggcgga atatctctca
     6001 cgcagaattt acggctgttc attccgaata ataccactga aaatcggcgt catcccatat
     6061 gccatagaaa acggcgacgt cgacttcatc ctctgtaatc ccacgtttta cgccatattc
     6121 tgccagcaga gaaacgtcac ccagctagcc accagaataa acaacgacac cggcagacca
     6181 ctcagccgtt atgcaggcac aatattctgc cgcactgaca acgaatccat ttactgcata
     6241 aacgatatca agggcagaat ttttgcgtgt ccgaacgcat attcgctggg cggctggctg
     6301 tccgtatggc gagaaatgct caagcaggat ttcaaccctc agactgacac aaaagaactc
     6361 aaaactttcg ccacccagga agaagtcgta aaagccgtcc tcaacggcac ggctgaagcc
     6421 ggctcggtac ggagcgacgt actggaatta atggccaaca gcaaacaaat tcatcctgag
     6481 gattttagga taatcaacag gaaaatggac gacttcgccc tgccgcattc aaccgaatgc
     6541 taccctgaat ggggattcgt ccagttgcaa catgtcccga acggcattgc caaacaagtc
     6601 acggtagcac tgttgcagat gacgccgcgc gattatgccg ccgtcgctgc cgaaatcgcc
     6661 ggctggactt cgcccgaaaa ctgccagccg gtaatcgaat gtctcgaata cctgaattta
     6721 agcccctata tcaagaccaa aaccgaaggc tggctcgtcg tcgttgacag atattggccg
     6781 attctgctcg gcggcggcat tatcatcctg acgtcgatga ttgtcgcagt attggccata
     6841 agggcaaaca tgaaataccg cgacctcaaa catgaactct cacagcgcca ggaccgcatg
     6901 tcggaaatat tcgactgctc agacgaagga ttagcctatt gcaataacaa cgacgaaatc
     6961 atttacgtca accagacatt ttgcaggctc accggctgta ccgaatctga acttacgggc
     7021 gacaatattt ttcaccggct gccgacggat ctctgtcact ccgcaagccg gctgatagac
     7081 gaatatcgca ataacaatta ctccaacccg catgaaatga cgtattgcat aaacggcata
     7141 gacgtagcga tgaaaataaa gcccttttac gccgacgaaa aatatcacgg cgcactattc
     7201 agccttgctg acataaacga tctcatgacg gctcgcaggg aaatcgcagc gataaatcat
     7261 gaactggaag actccattcg ccgagccaac cggttggcga tgcgggcact gcaggctgac
     7321 caggcaaaag caaacgccat aacaaatctt aaccgcgaca tacgcacgcc gcttaacgga
     7381 atcatggggc ttgccaacat tctcgaaaac acggaacttc agccgcagca gaaaaattat
     7441 atcgacatcc ttaaaaactc catcgacacc atggtaacgt tactggacaa ggcattggat
     7501 atgtcgcgcc tggaattcga gcaaatttcg ctggacagag ttctgttcga cgtaagagac
     7561 gagatatcgc agatattgaa acttctaaga tttcaggcgg cggaaaaggg actcagcctg
     7621 gattttaacg tagagaacga cgtgccgcag aaaatctgca attcacccga ccggctgcga
     7681 cagattctga taaatctggt aagcaactcg attcatcaca cggaaaaagg gaaaatcagt
     7741 ttgcgggtga cgtcaggcag gacggtaaat gacggctgcg agattaaatt cgcggtcagc
     7801 gataccggca taggaatatc gccggagaaa cttgaaacca tattcgacaa aattcccgcc
     7861 ggcgacaatc tgttggacgt tcatggcgga acagggctgg cactggcgat ttccagcaaa
     7921 atcgccagat tgatgggcgg cagtcttgtc gcggagagtt cgcctggtat cggctcgact
     7981 ttcacgctta cgattgtgga ttttcacagc agcggcacag cccgggaaac ccgcacgccg
     8041 gatacgtcgc attcagaaaa cgagggtgtc ctggaaggtt tgaaggttct catcgcagaa
     8101 gacaaccagg tcaacaggct ggtgctggac gagatacttt tgaaacacgg ctgcacagtc
     8161 acctgcgtcg aaaacggccg agccgctgtt gaagaagtgg ttaatgacca ttttgacctg
     8221 gtactgatgg attgtcatat gccggtattg gacggctttg aagcgacgct tgaaatacgc
     8281 cgtctcgctc cgccgttccg caatattccg atactcgcca tcaccgccaa cgcactgcca
     8341 caaataaagg actactgctt ccgctgcggc atgaacggtt atatatcaaa accgttttcc
     8401 gccgaaactc tcaaaaccgc cataattcga tgtctgagcg gcgcgccggc ggcagctaat
     8461 gaacgtgccg caaacacggc gcagaacgaa gaaacaatag acgtcgacca gcaggcactt
     8521 gttgaacgta tcggcaaaag cggcgacgta atgaaacacg tcgtggagtt gtttttggac
     8581 gactgcgtaa aaagcatcgc acagcttcac aagtgtttcg atgaaaacga catgacacaa
     8641 gccgccatgg tctctcattc gctaaaaggc tcatccgccc tcgcaggcgc cgctcgcata
     8701 cagaacgccg ccatggaagc cgaacaggcc gccctcgacg ccaacaggga atatctcggc
     8761 aaggtcattg aaataattga agaatgtttt aaaaaataca aaacagccgt atcggcttgc
     8821 atttgaggga acagtttata tactgtctga acgtcagcga ggatggcgga attggcagac
     8881 gcgctaggtt caggtcctag tggtcgtaag
//
LOCUS       DAONFI010000057         8854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9071_length_8854_cov_3.789862, whole genome shotgun sequence.
ACCESSION   DAONFI010000057 DAONFI010000000
VERSION     DAONFI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9071_length_8854_cov_3.789862"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..545
                     /locus_tag="PLK08_02925"
     CDS             114..545
                     /locus_tag="PLK08_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52483.1"
                     /translation="MASELKKELTERFVKGIHRSFTPCPLIGPKWLQEFPNSRPADYR
                     FYGIRKLAKAIGQSPEKILKTVMKNTSFKGLNVDVKIIGGIYVDIFDLEQQNRRLEAA
                     GMADHDSVGHEPRKQHRRRRHFFFRKRDDNCEEHQTMENQK"
     gene            621..2342
                     /gene="argS"
                     /locus_tag="PLK08_02930"
     CDS             621..2342
                     /gene="argS"
                     /locus_tag="PLK08_02930"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPS52484.1"
                     /translation="MNIKQTITDAVSDAMFKASGEKFDPQVNYAGKPQFGDYQANGCM
                     TAAKKLKCNPREFAQKVLANLDLGEMAGKVEIAGAGFINIFLSDEWLVSRLGEMVGDE
                     RLGAGRAADGPTVVVDYSGPNLAKEMHVGHLRSTIIGDALARVLEFLGNRVVRQNHVG
                     DWGTQFGMLLAHMHDMGEDSSASLSDLEGFYRDAKKRFDEDEDFARRARETVVALQGG
                     DESCRAAWKRFLDISLGHCQQVYDKLGVGLDASCVRGESSYNDDLPEVVNSLDAEGLL
                     SESQGARGVFLNEFTNKENEPAFIIVQKSDGGYLYATTDLAALRYRNGRLHAARILYV
                     TDSRQAQHFAQVFTIARKAGFVDEKISLEHVPFGMMLGNDGRPFKTRTGGTVKLMELI
                     EEARARAAELVRQKNPEIAAAELEKIADVVGIGALKYADLSQNRASDYVFAWNKMLSL
                     EGNTAPYMQYAYARVKSIFRKGGLESTAACRFILADDVERTLGVKLLQFGETLDAVAA
                     AAMPNILCAYLFELAGAFMSFYEKCPVLKADDAAVRVSRLALCDLTAGVIRQGLQLLG
                     IATLEQM"
     gene            2345..3322
                     /locus_tag="PLK08_02935"
     CDS             2345..3322
                     /locus_tag="PLK08_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52485.1"
                     /translation="MIIAGIDEAGLGPVLGPMVVSATAFAADVPEAADLWELLRGAVV
                     RKKSRRQGSAIVVGDSKKLFDRSRSDGLVPLERAVLAMLVNLPQFHGEMPSTLAALME
                     GVSAVPASVADEYPWYNTRQFGLPFQDNSISVKLAGNALKLAFRNSGVYFLGARVEPM
                     FVKQYNRYIAATNNKSTTAFDCTCRAIMWLWQSFVGQDMDVMVDRQGGRMHYREPLQR
                     IFCDASLKVLNEDEKLSSYDIEQDGRQMKVSFAVGGEELCLATALSSMMSKYIRELFM
                     EQLNAFWAGKLDGLESTAGYYVDGKRFYEQIKPLLDELGINEEMLLRSR"
     gene            3393..3722
                     /locus_tag="PLK08_02940"
     CDS             3393..3722
                     /locus_tag="PLK08_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS52486.1"
                     /translation="MSIQEWSEDVLLVQLNDDPQFTDEVNSLIERVRECPHSVVVDFA
                     GVTFINSSNIARLLRLRQAIQTCGGKLVLCKVNTQVWGVFLVTGLDKLFEFRNDVTTA
                     LASLQMA"
     gene            complement(3895..5178)
                     /gene="clpX"
                     /locus_tag="PLK08_02945"
     CDS             complement(3895..5178)
                     /gene="clpX"
                     /locus_tag="PLK08_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPS52487.1"
                     /translation="MANNKNRGNGPVMSCSFCGRSGKDAGVIIPGPDGLSICEQCVSL
                     CDNFLKQQKRVNRVSRISPDQIPSPKQIKEHLDQYVIGQEHAKKVLSVGVYNHYKRLT
                     HSEADSGDVEIDKSNILLIGPTGCGKTLLAKTLAKILNVPFAIADATTLTEAGYVGED
                     VENVLLKLVQAADYDIEAAQQGIIYIDEIDKIGKTSQNVSITRDVSGEGVQQALLKML
                     EGTLANVPPQGGRKHPEQQYIQVDTSQILFICGGSFIGLESIIRKRLGRQAIGFIGED
                     KPDNTPEEHGHTLSMVQPEDLVHFGMIPEMIGRLPCITALEPLSIEALMRILTVPRNA
                     LVKQYQKLFEMDDAELMFTDEALQAIARKAMKRNVGARALRSVVEELMLNLMFDLPER
                     QKIDGSYNITAEMVEAAVPVSLDEAFIPKRQKESA"
     gene            complement(5260..5622)
                     /locus_tag="PLK08_02950"
     CDS             complement(5260..5622)
                     /locus_tag="PLK08_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52488.1"
                     /translation="MLQKTQDNTIKSGMSDSNIINIVVSCAALVLIWAVAFVVIIKLK
                     KKLDPRLKRDEPFAGLRMEDLEKIHRAGMISDEEFASMRRSLLGLPPLDGDEKNKKIH
                     HPAAGFDIMKNLGKTDKN"
     gene            5994..6581
                     /locus_tag="PLK08_02955"
     CDS             5994..6581
                     /locus_tag="PLK08_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52489.1"
                     /translation="MSNAYCGSWKIDSSDRFIEIDEKFERFSRENPNFHFSGAKLGKS
                     FHDFLHSVQLSYFYRTLIYRVRYDNAMIELPVRCDTSALKRSYRMHISRLAGSATVEF
                     TLNLVKEEKREHISFFDLGTPRDEREIRVCFICGLVKGNGVWELPENHFRRAEFGGAV
                     FMPRLTHTVCKSCLSRTLGIYDDVARNQPAADVDS"
     gene            6628..7269
                     /locus_tag="PLK08_02960"
     CDS             6628..7269
                     /locus_tag="PLK08_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPS52490.1"
                     /translation="MILSAGKSRLSDVELLEQFVAGSSEAFGRLLCRYEKSLYAFLVR
                     YTGDMVLAEDIFQNTFLQVYQSAGLFDHGKSFKPWLYTIALNKARDALRQKKRQKAAS
                     IDAVSIGDDSDIDRSFADIIPSKILSPDEISVNRELRGVVQELIGQLPENLRAVLVMS
                     YFDRLPQKEIAEVLNVPVGTVKSRLHKAMAMFGQKWNAWAKKQNMNGDAELPK"
     gene            7266..8255
                     /locus_tag="PLK08_02965"
     CDS             7266..8255
                     /locus_tag="PLK08_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52491.1"
                     /translation="MSDMNEKNSPSLPEYDEQMLIDDILGELDEKAAAALRERSAAND
                     ALAARRNELAKIFKTLDFAGDIEAPQGLASRTMSWIESAKETSRLIGFEQFSGGYRPT
                     FSLRETLAVAAVLVIAVTVIFSTLTASRQRLWQNLCTSQMGEIGTAFQNYAMSNDSML
                     PTASSETPENWLNTSSNRPANSSALFRLLKQEYVHNPRVFQCPANGPRGFTVDREKSD
                     FPDRTFVGYSYQYSIRRCIRLDDSRLVVERFVILADQTPYFKNGHFDSSAGISRNHSA
                     GQSVLYADGHTAWVRTSDVGVSDDNIFLISSAATAYSGTEKPSDDNDSFLLPN"
     gene            8429..>8854
                     /locus_tag="PLK08_02970"
     CDS             8429..>8854
                     /locus_tag="PLK08_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HPS52492.1"
                     /translation="MLASCEGLHMPWEPPHTNFPTPQPQQPQQPPAAQKKPSQPIRQQ
                     NVSDEHRMLGGYYSWCKAQPFNKICFDNYNYIVHAFLAAGSDGKLQYNPARKIPSAEL
                     TALAHRNNVKIIISLGGGRNNYFDKITASRWATGRLVASA"
BASE COUNT         2072 a         2103 c         2412 g         2267 t
ORIGIN      
        1 aagcctgatg aatatgatgc tacgggtttt aacccgtaga gattcactaa aatattttta
       61 gaccgcgtta atgtgaagaa ttcgcctggg ttggtttaaa atacggacaa aggatggcgt
      121 cagagctcaa aaaggaactt accgaaaggt ttgtcaaggg tatacacagg agttttactc
      181 cgtgtcccct gataggcccg aagtggctgc aggaattccc caacagcagg ccggcggatt
      241 acaggtttta cggcatacga aaacttgcca aagccatcgg ccaatcgcct gaaaaaattc
      301 tcaaaaccgt catgaaaaac acatcgttca aaggccttaa tgtcgacgtg aaaataatag
      361 gcggaattta tgtcgatata ttcgatcttg agcaacagaa tcgtcggctg gaagccgccg
      421 gtatggctga ccacgattct gtcggccatg agccgcgcaa acagcatcga cgccgcagac
      481 attttttctt cagaaaacgt gacgataatt gcgaagaaca tcagacaatg gaaaatcaga
      541 agtagatttt tacagtttaa aaccaggtag catatttatc aggcttcggc ctgtccgcct
      601 gacggcggaa tttacgaaca atgaacatca aacagacaat tacggatgca gtttcggatg
      661 cgatgtttaa ggcatcgggc gagaagtttg acccgcaggt gaattacgcc ggcaagccgc
      721 agtttggcga ttatcaggct aacggctgca tgacagccgc caaaaagctg aagtgcaatc
      781 cgcgtgaatt cgcccaaaaa gttctggcca atctcgacct gggcgaaatg gccggcaagg
      841 ttgaaatcgc cggtgccggt tttataaaca tttttctgag tgacgaatgg ctggttagcc
      901 gtctgggtga gatggtcggc gatgaaagac ttggtgccgg cagggcggct gatgggccga
      961 ctgtcgttgt ggattattcc gggccgaatc tggctaagga gatgcacgta gggcatcttc
     1021 gcagtacgat tatcggcgac gcgctggccc gcgtgctgga gtttctgggc aatagggttg
     1081 tacgtcagaa tcacgtgggt gactggggta cgcagttcgg catgctgctg gcgcatatgc
     1141 atgatatggg cgaagattcg tcggcgtcgc tgagtgacct tgagggtttt taccgtgacg
     1201 ccaaaaagcg tttcgacgaa gacgaagatt tcgcccgccg cgcacgtgag actgtcgtag
     1261 cgttgcaggg cggcgacgaa tcgtgccgcg ctgcatggaa acggtttctg gacatctcgc
     1321 tcggccactg tcagcaggtg tacgacaagc taggcgtcgg ccttgatgcg tcgtgcgtac
     1381 gcggcgaaag ttcctataac gacgacctgc ctgaggtggt gaacagcctt gacgccgaag
     1441 gtcttttgag cgagtcgcag ggcgccaggg gcgttttcct gaatgaattc accaacaagg
     1501 aaaacgaacc tgcgttcatc atcgtacaga aatcggacgg cggatatctt tatgcgacca
     1561 ccgacctggc tgctttgcgg tatcgcaacg gcaggttgca tgcggctcgt attttatatg
     1621 tcacagacag ccgccaggct cagcattttg cacaggtttt caccatcgcc cgcaaagcag
     1681 ggtttgttga tgaaaaaatt tcgctggagc atgtgccttt cggcatgatg ctcggcaacg
     1741 acggccggcc gttcaagacg cgaaccggcg gaacggtcaa acttatggaa cttatcgaag
     1801 aagcccgtgc ccgcgccgcc gaactggtca ggcagaagaa tccggaaatc gccgctgccg
     1861 aacttgaaaa aatcgccgat gtcgtcggca tcggtgcgtt gaaatatgcc gacctttcac
     1921 agaatcgtgc gagcgattac gtttttgcct ggaacaagat gctttccctc gaaggcaata
     1981 ctgcgccgta catgcagtac gcctatgccc gcgtcaagag catatttcgc aagggcgggc
     2041 tggaatcgac ggcggcgtgc aggtttatcc tggcagacga tgtcgaacgt acgcttggcg
     2101 tgaaactgct gcaattcggc gagactctcg acgcggtggc tgccgcggcg atgccgaaca
     2161 tactttgcgc ctacctgttc gaattggctg gcgcgttcat gagtttttat gaaaaatgtc
     2221 ccgtactcaa agccgatgac gcagccgttc gcgtcagtcg gcttgctttg tgcgacctga
     2281 cggcgggcgt tatccgtcag ggattgcaat tgctgggcat agcgacgctg gagcaaatgt
     2341 aagtgtgata atcgcaggca tagacgaagc aggtctgggg ccggtacttg ggcctatggt
     2401 cgtttcggcg acggcttttg cggctgacgt gccggaggct gccgatttgt gggaactttt
     2461 gcgcggggct gtggttcgaa agaaatccag gcggcagggc agtgcgatag tggtcggcga
     2521 cagcaagaag ctgtttgacc gcagccgcag cgacggcctt gtgccgctcg aacgggctgt
     2581 gctggcgatg ctggttaatc tgcctcaatt tcacggagaa atgccctcca cgctggcggc
     2641 gcttatggaa ggcgtatcag ccgtgccggc gtcggtggct gacgagtacc cgtggtacaa
     2701 caccaggcag ttcggcctgc cgttccagga taactccata agcgtaaaac tcgccggcaa
     2761 tgcgcttaag ctggcattca gaaattcggg cgtatatttt ctcggagccc gcgttgagcc
     2821 gatgttcgtc aagcagtata accgctatat cgcagcgact aacaacaagt cgacgacggc
     2881 ttttgactgt acgtgtcggg cgataatgtg gctgtggcag agtttcgtcg gccaggatat
     2941 ggacgtgatg gtcgaccggc agggcggaag aatgcattat cgtgaaccgc tccagcggat
     3001 attttgcgac gcatctctga aagttctgaa cgaagacgag aaactaagtt catacgacat
     3061 cgagcaggac ggccgtcaga tgaaagtttc ttttgccgtc ggcggagagg aactgtgtct
     3121 cgcaaccgcg ctgtcgtcca tgatgagcaa atacatacgc gagttgttca tggagcagtt
     3181 gaatgcattc tgggcgggca aactcgacgg ccttgagtcg actgcgggat attatgtcga
     3241 cggcaaaagg ttttacgaac agataaaacc attgctggac gaactcggca taaacgagga
     3301 aatgctgctg cgaagccgat aatttttgct tgttgcggat acggggtata cgttattatg
     3361 acagtcagcc acgaaattca aggagatgaa atatgtcaat acaggaatgg tcggaagatg
     3421 ttctgctggt acaactgaac gacgacccgc aattcacgga cgaagtcaat tcactgatag
     3481 agcgtgtacg cgaatgtccg catagcgtgg tggtcgattt tgccggcgtg acgttcataa
     3541 attcgtcgaa catcgcaagg ctgttgcgtc tgcggcaggc cattcagacc tgcggcggca
     3601 agctggtgct ctgcaaggtc aatactcagg tatggggcgt attcctggtt accggccttg
     3661 acaagctgtt tgaattcagg aacgatgtga cgactgcatt ggcatcgttg caaatggcgt
     3721 aataaaaaac ttttgctggc ggcagagagc cggataaccc gacatccttt ctccgacaga
     3781 tttttttaaa aaaatattct gccttttttc tgccgtcgcc aggctgcaaa aaatccgatt
     3841 acaggcagcc aatgtttttg ggataggttt tagggcattt taaacttgct ctaattatgc
     3901 cgattctttt tgccttttcg ggataaacgc ctcgtccagc gataccggca cagccgcttc
     3961 aaccatctcc gcggttatgt tgtaactgcc gtcgattttc tgccgctccg gaaggtcgaa
     4021 cataagattc agcataagct cttccacaac ggaacgcaga gcgcgcgcgc cgacgttgcg
     4081 tttcatggct tttctggcga tagcctgtaa agcctcgtcg gtaaacatga gttccgcgtc
     4141 gtccatttca aaaagtttct ggtactgttt taccagtgca tttcgcggta cggtaagtat
     4201 tcgcatgaga gcttcgatgc tgagcggctc gagtgcggtt atgcatggca gacggcctat
     4261 catttccggt atcatgccga agtgcaccag atcttccggc tgaaccatgc taagcgtgtg
     4321 gccgtgctcc tcaggcgtat tgtctggttt gtcttcgcct atgaagccga ttgcctggcg
     4381 gcccagccgc ttgcgaatta tgctctcaag gcctatgaac gagccgccgc aaataaacaa
     4441 tatctgcgac gtgtcaacct gaatgtactg ctgttcggga tgcttcctgc cgccctgcgg
     4501 cggcacatta gccagcgtgc cttccagcat tttcagcaac gcctgctgga cgccttcgcc
     4561 ggaaacgtcg cgggttattg aaacgttctg cgacgttttg ccgattttgt cgatttcgtc
     4621 gatgtagatg attccctgct gggcggcttc gatatcgtaa tcggcagcct gtaccagctt
     4681 gagcaggacg ttttcaacgt cttcgccgac atagccggct tcggtgagag tggttgcgtc
     4741 ggcaatcgcg aacggcacgt tcagtatctt tgccagcgtt ttagccagca gcgttttgcc
     4801 gcagccggtg ggcccgatga gtagaatatt cgacttgtcg atttcaacgt cgcctgagtc
     4861 agcctcggaa tgcgtcagtc gtttgtaatg attgtaaacg ccgacggaaa gaaccttttt
     4921 ggcgtgctcc tggccgatta cgtactggtc tagatgctcc tttatctgct ttggcgacgg
     4981 tatctggtcg ggcgatatac ggctgacgcg attgacccgt ttctgctgtt tcaggaagtt
     5041 atcgcagagg cttacgcact gttcacagat tgacagcccg tcagggccgg ggattattac
     5101 gccggcgtct ttgccgctcc tgccgcaaaa actgcaactc attaccggcc cgttgccgcg
     5161 attcttgtta ttcgccatat cgtttgtacc ttgtcgactt cttattaatc ctgtatttat
     5221 ctttggatag gagctgtttg gacatgtttt gaacagcttc tagtttttat cggttttgcc
     5281 gagatttttc attatatcaa aacctgcggc tgggtggtga atttttttgt tcttttcgtc
     5341 gccgtcgagc ggcggcaggc ccagcagcga cctgcgcata cttgcgaatt cctcgtcgct
     5401 tatcatgccg gccctgtgaa ttttttccaa atcttccatg cgtaagccgg caaaaggctc
     5461 gtcgcgtttc agccgcggat caagtttctt cttcaatttg ataatcacga cgaatgctac
     5521 agcccatata agtaccaggg ctgcacagct tacgacgata ttgatgatat tgctgtcgga
     5581 catgcctgat tttatagtat tatcctgcgt cttttgcaac ataagatatt ccgaatgatg
     5641 cacaatcgct ataatgatat gacactacgt tggttatgca tgccttgtga aatgtgtaac
     5701 accgaaaata aaacaaattt tttccgatac ggcaatttgg gttttccatt gataaaacgg
     5761 ggcaaaacta ttagatgcat cgaattagtg gaattggggt gtgtaaaaat tatcttgaaa
     5821 tatgtaatag cctcatttat aagaccttat gtaacgaagg caaatttctg agaggaattg
     5881 atacagctgt tgatgaaaat ttggcggttt tttgtggtct gtgcaattgc gtcaatgaca
     5941 gatgagttgg ttttgggttg cagtaaaaaa tttccggtaa tcaggacaaa aaaatgtcaa
     6001 acgcttattg tggcagctgg aagatagact cttccgacag atttattgaa attgacgaaa
     6061 aatttgaacg tttttcgcgt gaaaacccca attttcattt ttccggcgcg aagttgggca
     6121 aaagttttca tgattttctg cattccgttc agctttcata tttttatcgc acgctcatat
     6181 atcgtgtccg ctatgacaat gccatgattg agctgccggt gcgttgtgat acgtcagcgc
     6241 tgaaacggag ttaccggatg catatatcaa ggcttgccgg ctcagctacg gtcgagttca
     6301 cattaaatct ggtaaaagag gaaaaacggg agcatattag tttttttgat ttgggcactc
     6361 ccagagatga gcgtgagatt cgcgtgtgtt tcatctgcgg gctggtgaag ggcaacgggg
     6421 tttgggaatt gccggaaaat catttcaggc gagcggagtt cggcggcgcg gtttttatgc
     6481 cgcggcttac ccatactgtc tgtaaaagct gtctgagccg gacgttgggt atctatgacg
     6541 atgtagcacg caatcagccg gcggctgacg tggatagctg aaataattgt tttgctatat
     6601 tccattcgtc agttattata agctgcgatg atattatccg ccggaaaatc caggctcagc
     6661 gatgtggaat tgttggagca gtttgtcgcg ggcagcagcg aggcttttgg ccgattgcta
     6721 tgccgttatg aaaaaagtct ttatgcgttt cttgtgcgtt ataccggcga tatggtgttg
     6781 gcggaggata tttttcagaa cacttttttg caggtttacc agtctgccgg gctttttgac
     6841 cacggcaagt cgttcaagcc gtggttgtac acgattgccc tgaataaagc tcgagacgct
     6901 ctccgccaga aaaaacgtca gaaggctgcg tcaatcgacg cggtatccat aggcgacgat
     6961 agcgatattg accgttcgtt tgccgacata attccttcaa aaattctttc tcccgatgaa
     7021 atttccgtga accgtgagct gcgcggcgtc gtacaggaac tgatagggca gttgccagaa
     7081 aatctgcggg ctgttctcgt catgagttat tttgacaggc tgccgcagaa ggagatagcc
     7141 gaagtcctga acgtgccggt aggtacggtc aagtcccggc tgcataaggc tatggcgatg
     7201 tttggacaga agtggaatgc ctgggctaag aagcagaaca tgaatggaga cgcggagctt
     7261 ccgaaatgag cgatatgaac gaaaaaaact ctccatccct gccggaatat gacgagcaga
     7321 tgctcatcga cgacattctc ggcgaacttg acgagaaggc tgccgctgct ctgcgcgagc
     7381 ggtctgccgc taacgatgcc ctcgccgctc gtcgaaatga acttgccaaa attttcaaaa
     7441 cgcttgactt cgccggcgat attgaagcgc ctcaagggct ggctagccgg acgatgagct
     7501 ggattgaatc ggcaaaggaa accagcaggc ttatcggctt cgagcagttt tccggcggat
     7561 atcgccccac gttttcatta cgcgagactc tggctgtcgc ggcggtgctg gttatcgccg
     7621 ttacggtgat tttttcaacg ttgacggcgt cgcggcagcg gctgtggcag aatctctgta
     7681 cctcgcagat gggcgaaatc ggtactgcgt tccagaatta tgcgatgtcg aacgatagta
     7741 tgctgccgac ggccagcagt gaaacgccgg aaaattggct taatacatcg agcaatcggc
     7801 cggctaattc gtccgcgctt ttccggttgc taaagcagga atatgtccat aatccgcggg
     7861 tttttcaatg tccggctaac gggcccaggg gttttacggt cgacagggaa aagtcggatt
     7921 ttcctgaccg tacttttgtg ggctattcgt atcaatattc cattcgtcgg tgtatacggt
     7981 tggacgattc cagactggta gtcgaaagat tcgtgatttt ggctgaccag accccatatt
     8041 tcaaaaatgg ccactttgat tcttccgccg gcataagcag gaatcattct gccggccaga
     8101 gtgttttata tgccgacggc catactgcat gggtgcgcac ttccgacgtc ggcgtcagcg
     8161 acgacaacat ttttctgata agttctgcag ccactgcgta cagcggcacg gaaaagccgt
     8221 ccgacgataa cgacagcttc ctgctgccga actgaagtcg tttctgaaat tcaattttaa
     8281 gagccgcttt taaaattgtc ggctgagccg tcattccgcg tttccggcgc gatgttttac
     8341 tttcatttac gcaggttttg cattatactt tcgccgaaaa cagatgagaa ggattgtata
     8401 tacaacatgg atgttggcgg gctgttttgt gttggcttcc tgtgaaggtc tgcatatgcc
     8461 gtgggagccg ccgcatacga attttcctac gccacagccg cagcaaccgc agcagccgcc
     8521 tgccgcacaa aaaaaacctt cacagccgat tcggcagcag aatgtttccg acgaacaccg
     8581 catgctcgga ggttactatt catggtgcaa ggctcagccg ttcaacaaaa tctgttttga
     8641 taattataat tatatcgttc acgcctttct tgccgccggc agtgacggca aattgcaata
     8701 taatccagcc cgaaaaatcc cttccgccga gcttaccgct cttgcgcatc gtaacaatgt
     8761 aaaaatcatc atttcgctcg gcggcggccg gaacaattat tttgataaaa taactgccag
     8821 ccgttgggct accggccgtc tcgtggcatc tgcc
//
LOCUS       DAONFI010000058         8829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9117_length_8829_cov_4.001596, whole genome shotgun sequence.
ACCESSION   DAONFI010000058 DAONFI010000000
VERSION     DAONFI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9117_length_8829_cov_4.001596"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1123
                     /locus_tag="PLK08_02975"
     CDS             272..1123
                     /locus_tag="PLK08_02975"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPS52493.1"
                     /translation="MAKKESYEFVRLVGDERRVGPTLASVSRHWRKGRECFLFLSPHD
                     DDVIIGGGLLIQELIREKVPVHLAVVTDGAMGYCSMKQKKTIAQIRAQETIDACRILG
                     IPQKNIHLLQFPDCQLANFMGRRPAVENDPAVIEGFTGLQNAFTALLRKVQPTQVFLP
                     TNTDIHPDHRIVHSELLISAFHSSGDIWPELGETLKDTPYIHEMAIYCNFPTRPKLRL
                     TAPLGVLENKLRAIAAFKSQKQIKSLVNIIRHGGPLEYFRPVEFQLYHPHIYRDQFEE
                     TPMANLF"
     gene            complement(1182..3836)
                     /locus_tag="PLK08_02980"
     CDS             complement(1182..3836)
                     /locus_tag="PLK08_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPS52494.1"
                     /translation="MKKILVRFLVWLAGKILAMRYRIKIAGMDEILKKGRTGILFLPN
                     HPGLIDPIILMAHLYNKFGIRVLADRHQVDRFFIRNMVSLMNIIPIDDVEKTGVLAVD
                     DIRNKVNICADVLKSGENLLVYPAGRIYRSFMEDLRGNSGAYTLIREVPQTRVVLVRT
                     SGLWGSSFTWASGEAPSVAAAFRKGFKGALKSFIFFMPRRKVTIELFEPTDIPCDDDK
                     TRLNRYLENFYNAAAQPNTYVPYSIWQRGGTQILPEPKFSIFTADPSLVPQHIRDEVL
                     GYIEELTFCSRDELKDSTRLAHDLGMDSLTGMELITWLQKEYGFVPDDIEDINTVGDV
                     MLAAAGEFVKHTAKKIKPVQKKWFTGMAEGCMPAGVRNMTIPQAFLKKAVRNPSSPII
                     ADQVAGVKTYRDMVLGLMVLVPEFRKLDGEYLGLMMPASVTANLMYMAALFAGKIPAM
                     VNWTLGRKNLEHCLKLVNMEKILTSRMVADRLRGQGVDLAAVESRFVYIEDIAASLGR
                     VKKIAALVASRLCWILLRKALKNVKHTAVVLFTSGSENFPKAVPLTHKNILTNLDSVL
                     GEAITRKLGPNDVLLGMLPPFHSFGITVCTVLPLIHGTRVAYSPNPTDGVTIAHCVNE
                     YKITLLVGTPTFLNGIMKSSSNRQLESLQLIVTGAEKCTKKVYDLFGRRCPKAVIMEG
                     YGVTECSPVVSVNHDNDHTAGTIGKVIGAFDFMILNPETHEELPHGQTGLLVVAGDCV
                     FGGYMNYDGASPFLKINGREWYVTGDLVFEYRKGGWLTFAGRLKRFIKLGGEMISLPA
                     IEAVLLEHFSKDSDDGPCLAVMASKKDEPEIIMVATRDDIGKEDANAVIRAAGMSGLH
                     SIRVLRKMPALPLLGTGKTDYRTLAGMI"
     gene            complement(3833..5110)
                     /locus_tag="PLK08_02985"
     CDS             complement(3833..5110)
                     /locus_tag="PLK08_02985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPS52495.1"
                     /translation="MDADIRLQVKRARGRFAAMAATYGLGVFNDGFFRQSAIFLAISS
                     QHKGLEGWIMSLFTLPYLVSASYAGWLADRFSKRNVIIGAKCMELAAMLMGAYGIMFD
                     DWAFILMMAFTMGWQSCLFSPALNGSIPEIYPAFYVNRANSFLKVIVTVMILAGVSVS
                     GVAIGTGGMGPWDIPLGRFIVGVGVVSVSLIGLATSFFVTSKPPADPHVRFPWNGPLD
                     TLRPIIRIFSDRLLWYVMLADVFVWFAGSIMVLCLSQMAKVQFGWGEATGGYIIAVEL
                     VGLAVGGLLSNILTRYRNWYRVLGPLAVIMGLLLGCCVFLPAVMQEGYRKMAAFVLFG
                     AVGVIGGLYMIPCEAFIQIRPAGKCKGTVIATANFAVFVGIFLSGPVEVFMIRFLRPT
                     DTMAVVGIVGIAVGLYISLKLLRTVDTQKEFEL"
     gene            complement(5124..7307)
                     /gene="rlmKL"
                     /locus_tag="PLK08_02990"
     CDS             complement(5124..7307)
                     /gene="rlmKL"
                     /locus_tag="PLK08_02990"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HPS52496.1"
                     /translation="MNDMELVATSTFGLESLVSRELETLGIASRITQSGRIHFNGDIR
                     TLATANVHLRTADRVLLMMGQFEAADFGELFDRTYAIDWQELIPADGAFPVLGRSVKS
                     VLTSVPACQKIVKKAIVEKLRAAHGVSELPETGAVFTVEVALLKDVATITVDTSGAGL
                     NKRGYRPIVGVAPLKETLAAAMVMLSFYCPQRPLIDPFCGTGTIPIEAALIARNIAPG
                     VNRSFAAENWPTVGRGIFDEIRAAARDLVLREPIEQLMGTDTDGDAVKLARRNAQAAG
                     VADDIHFQERPFSELRSKKDYGVIITNPPYGVRVGEARELQSLYRSFPEVLRRLPTWS
                     HYILTAYPDFEQIVGQPASRRRKLYNGRIECTFYQFHGPRLGGGRKETDAPDEQLQPS
                     RQASVQHGGNEQAFGGLHENAAGQAEMFANRLAKNARHLRKWQSRGIECYRIYDKDIP
                     EVPLVVDRYGPALHIAEYVRPDELTPAEHADWLEMLAKTAARTLDVPAADVFLKRRNR
                     QKGANQYERLADEAREYVVMENGLKFRVNLSDYLDTGLFLDHRIARQMVRNEAAGKRF
                     LNLFAYTGSFSVFAAAGGAKSTTTVDLSQKYLDWAEVNMTLNGFAGPQHEFVRCDAVG
                     FLQGHPRVPSYDLVVFDPPTFSNSKSLEEDFDVQQNYLHVLELLAGVVTLNGVVYFST
                     NFRRFKFDEQAAANAGWNVREITRQTLPEDFRNKRIHRCWRMVKV"
     gene            complement(7300..8217)
                     /locus_tag="PLK08_02995"
     CDS             complement(7300..8217)
                     /locus_tag="PLK08_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52497.1"
                     /translation="MCRSLLQIFLTLNILLVVGCENIGVITAGQDNSVKPAETSAVSK
                     MPDTGTKISSAAPDKSQPQRPTRMRREFKVIRLEVNSIELPAGTAGRLEKFWTFLDEE
                     PVSLHSTVLGMNGFRVGVGRNCDWAEVESLLARQGGLSFQKKIIETRLGYPAHLEMKT
                     GQPVQNIFIFSDAQTLRGEHFPPGDNLLTLSTFLDEETNRDIIIVAVPQIRSTVRHFG
                     IETDNDDPRIVYKSQFYPLDEMAFRVKMPTDDFLVIGPGSQASRPNSVANHFLTCTKQ
                     GMDYDRIFVIRPYVIRVPFGNASNGAGDE"
     gene            complement(8223..>8829)
                     /locus_tag="PLK08_03000"
     CDS             complement(8223..>8829)
                     /locus_tag="PLK08_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52498.1"
                     /translation="CTVKSVPAAVHTSATPTTMPAETAAAAVAACATEPATSFMTKTS
                     ECTRQRTINWQYALNWVLAATKIIAFAAGGFLFLLQTVAGHLSLVGPAVRGIRQIISS
                     VCWAAVLLAMLTPWQQLLRPSHVCGATFNLGDIVHCRDIATGAGFAVLIPYYTRFIAY
                     PCIAILAWLMIVVKFARGYYRLTGPQPAATVNMATKCDEVK"
BASE COUNT         2120 a         2539 c         2172 g         1998 t
ORIGIN      
        1 catgcttcgc agaacgctaa gttcatataa cgtgcaggaa gaagtgccgt cgctcataaa
       61 agccctcgaa cggttcaaaa ccaatgaaga attcctccgc tcgctcgacg taagccgaat
      121 gagttaaacg ttaattttca aaaaacgccg ccaaatgacg gcgttttttt atgcgcttga
      181 aaatacggcg accagttttt ttatgttgaa tttgagaacg taaattgata atcagtttat
      241 gttcgtatca tttttatgga gattttccca aatggccaaa aaggaatcat atgaattcgt
      301 gcgtctggtc ggcgatgaac gccgcgtagg tccgacgctt gcctccgtat cgcgacactg
      361 gcgtaaagga cgtgagtgtt tcctgttcct ttcgccacac gacgatgatg tcatcatagg
      421 cggaggcctt ctgatacagg aactcatccg cgaaaaagtt ccagtacatc tggccgtagt
      481 cactgacggt gcgatgggtt actgctccat gaagcagaaa aaaaccatcg cacagatacg
      541 tgcacaggaa actatcgatg cctgcagaat tctgggcatt ccgcagaaaa atatccatct
      601 gcttcaattt cccgactgcc agttagccaa cttcatgggc cgtcggccgg cagtggaaaa
      661 tgatcctgcc gtcatcgaag gatttaccgg cttgcagaat gcctttaccg ccctgctccg
      721 caaagtccag ccgacgcagg tttttctgcc gacaaatacc gacatacatc ccgaccatcg
      781 catagttcat tcggaacttc tgataagcgc gtttcattcg tccggcgata tctggccgga
      841 actcggcgag acgctcaaag atacgccgta catacacgag atggccattt actgcaactt
      901 tcccaccagg cccaaacttc gtctgactgc accgctgggc gtacttgaaa acaaacttcg
      961 cgccatcgcg gcgttcaagt cgcaaaagca gataaaatcg ctggtcaaca tcatccggca
     1021 tggcgggccg ctggaatatt tccggccagt cgagtttcag ctttatcatc cgcacatata
     1081 ccgcgaccag tttgaggaaa cgccgatggc taacctgttc tgacctcggg cagagtttgt
     1141 acacggtttt caaaaataat tttgagagcc gctatagaat ttcaaatcat gccggctagc
     1201 gtgcgataat cggtcttgcc cgtaccgagc agcgggagcg caggcatttt gcgaagaaca
     1261 cgtatactat gcaggccgct catgccggcg gcacgtatga cggcattggc gtcttccttg
     1321 ccgatatcgt cgcgcgtagc gaccatgatt atttccggct cgtctttttt tgacgccatt
     1381 accgccagac aggggccgtc gtcggaatct ttggaaaaat gttccagcaa cacagcctct
     1441 atcgccggca gagatatcat ttcaccgccc agttttataa accgcttcag ccggcctgca
     1501 aacgtcagcc agccgccctt gcgatactcg aaaacaaggt cgcccgtaac ataccattct
     1561 ctgccgttta ttttcaaaaa cggcgatgct ccgtcataat tcatatagcc gccgaatacg
     1621 caatcgccgg caaccaccag caggcctgtc tggccgtgcg gcaactcctc gtgcgtctca
     1681 ggattcagaa tcatgaaatc aaacgcccct atgaccttgc cgatagttcc cgccgtatgg
     1741 tcgttgtcgt gattcactga aaccaccggc gaacattccg tcacgccata gccctccatt
     1801 atgacagcct tcggacaccg ccggccgaac aggtcgtaaa cctttttcgt gcatttttcc
     1861 gcacccgtca caataagctg cagggactca agctgcctgt tcgacgacga tttcattatg
     1921 ccgttcaaaa acgtcggcgt gccgacgagc agcgtaatct tgtattcatt cacgcagtgc
     1981 gctatcgtca cgccgtcggt cggattcggc gaataggcca ctcgcgtgcc gtgtatcagc
     2041 ggcagaaccg tgcagacggt tatgccaaac gaatgaaacg gcggcagcat gccgagcagt
     2101 acatcgttcg ggcccaactt cctggtgatt gcttcaccga gcaccgaatc gagattcgtc
     2161 aaaatattct tatgcgtcag cggaactgct ttggggaaat tctccgaccc gcttgtaaaa
     2221 aggacgacag ctgtatgctt gacgtttttg agtgcttttc gtagcaaaat ccagcacagg
     2281 cggctggcga caagagccgc gattttttta actctgccca gcgaagccgc tatgtcttcg
     2341 atatagacga atctgctctc gacggcagcc aggtcgacac cctggccgcg cagccggtca
     2401 gccaccatgc gtgacgtcag gattttttcc atattcacga gttttagaca gtgttcgaga
     2461 ttttttctgc ccaacgtcca gttcaccatc gccggaattt tgccggcaaa cagcgcagcc
     2521 atatacatca ggttcgccgt caccgaggca ggcatcatca agcccagata ttcgccgtcc
     2581 aacttgcgga attccggcac aagcaccatc aggccgagca ccatgtcgcg ataagtcttt
     2641 acacccgcaa cctggtcggc gatgataggt gaagacggat tacgtaccgc ctttttcaga
     2701 aacgcctggg gaatagtcat gttccgtacg ccagccggca tacagccctc tgccatgccg
     2761 gtgaaccatt ttttctgaac gggtttgatt tttttcgcgg tatgtttgac gaattcgccg
     2821 gcggcagcca gcatcacgtc acccacggta ttgatatcct cgatatcgtc cggcacaaaa
     2881 ccgtactcct tctgcagcca cgtgataagt tccataccgg tcaacgaatc catgcccaaa
     2941 tcatgtgcca gccgcgttga atccttcagt tcgtcgcggc tgcagaacgt gagctcctcg
     3001 atatagccga gcacttcgtc gcgaatatgc tgcggcacaa gcgaagggtc ggcggtgaaa
     3061 atgctgaact tcggttcggg aagaatctgc gttccgccgc gctgccatat ggaatacggc
     3121 acataagtat tcggctgggc ggcagcgttg tagaaatttt ccagatagcg gttgagtctg
     3181 gtcttgtcat catcgcaagg aatatcggta ggctcgaaaa gttcgattgt aactttacgg
     3241 cgcggcatga aaaatatgaa acttttcaat gcacctttaa agcctttcct gaatgccgcg
     3301 gctacggacg gagcttcgcc tgaagcccac gtgaaactgc tcccccacag gccactggtg
     3361 cggacgagaa caacgcgcgt ctgcggcact tcccttatga gcgtatatgc accgctgttg
     3421 ccgcgcaaat cctccatgaa cgaacgataa atgcggccgg ctggataaac cagcagattt
     3481 tcaccgcttt tgagcacatc ggcacagata ttgaccttat tgcggatatc atccacagcc
     3541 agcacgccgg ttttctcgac gtcgtctatc gggatgatat tcatgaggct gaccatattg
     3601 cgtatgaaaa acctgtctac ctgatggcgg tcagcgagca cccgtatgcc gaatttatta
     3661 taaagatgcg ccataaggat tatggggtct atcaggccgg ggtggttcgg cagaaacagt
     3721 atgccggttc tgcctttttt gagaatttca tccatgccgg cgatttttat cctgtaacgc
     3781 atggctaata tctttccggc tagccagacg agaaatcgca ccaagatttt tttcacagct
     3841 caaactcctt ctgcgtatca acagttcgca aaagcttgag acttatataa agtcctacag
     3901 caattccgac aatccctacc acagccatcg tgtcggtagg tcgcaaaaat ctaatcataa
     3961 aaacttcgac aggccctgac aaaaaaattc cgacaaacac tgcaaaattg gccgtcgcga
     4021 taaccgtgcc cttgcacttg ccggccggcc gaatctgtat gaacgcctcg cacggaatca
     4081 tgtaaagtcc gccgattacg cccaccgccc caaacagcac aaaagccgcc attttacgat
     4141 acccctcctg cattacagcc ggcaaaaaca cgcaacaacc cagcagcaga cccataatca
     4201 cggctaacgg cccaagcaca cgataccagt tacgatatcg cgtcagtatg tttgacagaa
     4261 gtccgccgac tgccaaacct acaagttcta ccgcgattat ataaccgccc gtcgcctcgc
     4321 cccagccgaa ctgtacctta gccatctggc tgagacacag taccattatt gaacctgcga
     4381 accacacaaa tacgtcggca agcatcacat accataacag cctgtcgctg aaaatcctga
     4441 taataggccg cagggtatcc agagggccgt tccacggaaa ccgtacatgc gggtcagccg
     4501 gcggctttga cgtcacgaaa aaactcgtcg ccaaccctat gagcgaaaca ctcaccacac
     4561 ccacgccaac tataaatcgg cctagcggaa tatcccacgg gcccatgccg cccgtgccta
     4621 tcgccacgcc ggatacggat actcccgcaa gtatcatgac cgttacgata accttcagaa
     4681 acgaattggc ccgattgacg taaaatgccg gataaatttc cggtatcgac ccgttgagag
     4741 ccggactgaa caggcagctc tgccagccca tcgtaaaagc catcatgagt atgaaagccc
     4801 aatcgtcgaa cattatcccg taagccccca taagcattgc agccagttcc atgcatttcg
     4861 cgcctataat cacattgcgt ttggaaaatc tgtccgccaa ccagccggcg taagaagcgg
     4921 ataccagata cggcaacgtg aaaagcgaca taatccagcc ttcaaggccc ttgtgctggg
     4981 acgagatagc cagaaatatc gcactttgtc ggaaaaatcc gtcgttgaac acaccaaggc
     5041 cgtaggtggc ggccatcgcc gcaaaacgcc ctcttgcacg tttcacctgc aacctgatat
     5101 cggcgtccat gaataaaaat ccctcacact tttaccattc gccagcagcg atggattctt
     5161 ttattgcgga aatcttccgg cagcgtctgc cgcgttatct cccgcacgtt ccaacctgca
     5221 ttcgccgccg cctgttcgtc gaatttgaat cgtcggaaat tggtggaaaa ataaactacg
     5281 ccgtttaacg taacaacgcc ggccagcaat tcaagaacat gcagataatt ctgctgtacg
     5341 tcgaaatcct cctccaggga tttggagttg gagaatgtcg gcggatcgaa cacgaccagg
     5401 tcgtaactcg gcactctcgg atggccctgg aggaaaccca cggcatcgca tcgcacaaat
     5461 tcatgttgcg ggccggcaaa gccgttgagc gtcatgttca cttcagccca gtccagatat
     5521 ttctgcgaca agtcgacggt agtggtgctt ttcgctccgc cggcggcggc aaagacgctg
     5581 aatgagccgg tgtatgcaaa aagattcaaa aaccgtttgc cggcggcttc attgcgaacc
     5641 atctgccgcg ctatgcgatg gtcgagaaac agacccgtat ccagataatc agagaggttt
     5701 accctgaatt tcaagccgtt ctccatgacg acgtattccc tggcttcatc ggcgagacgt
     5761 tcgtactgat tagcgccttt ctgtcggttt ctgcgtttca gaaagacgtc agccgccggc
     5821 acgtcgagcg ttcgagccgc cgttttagcc agcatttcca gccaatcggc gtgttcagcc
     5881 ggcgtcaatt catccggccg tacatattcc gcaatatgca gcgccggccc ataacggtcg
     5941 accactagcg gcacttcggg gatatccttg tcgtatatcc tgtaacattc gatgccgcgg
     6001 gactgccact tacggagatg ccttgcgttt ttcgccagcc ggtttgcgaa catttccgcc
     6061 tgtccggcgg cattttcgtg caagccgccg aaagcctgct cattcccgcc atgctgaaca
     6121 gacgcctgcc gtgacggctg cagttgttca tcgggagcat ctgtttcctt gcgtcctccg
     6181 ccaagccgcg gcccatgaaa ctggtaaaat gtgcactcaa ttctgccgtt gtaaagtttg
     6241 cggcgacgcg aagccggctg gccgactatc tgctcaaaat cgggataggc cgtaagtatg
     6301 tagtgcgacc acgtcggcag ccggcggagc acttccggaa acgagcggta aaggctttgc
     6361 agctcgcgtg cctcgccaac tcgcacgcca tacggcggat tggttataat cacgccgtaa
     6421 tctttcttgc tgcgaagttc gctgaagggc cgttcctgga aatgaatatc gtcggctacg
     6481 ccggcagcct gggcgttgcg gcgggcaagt ttgacggcat cgccgtcggt atccgtaccc
     6541 atgagctgtt caatcggctc gcgaaggaca aggtcacgtg cggcagcgcg aatttcatcg
     6601 aaaattccgc gtccgaccgt cggccagttt tcagccgcga aactgcgatt cacgccaggc
     6661 gcgatatttc tggctatcag cgcagcctca ataggtatcg tacccgtacc gcagaacgga
     6721 tctatcaacg gccgctgcgg acagtaaaaa ctcaacataa ccatcgcagc cgccagcgtc
     6781 tccttaagcg gcgcaacgcc gactattggc cgataaccgc gtttgttcag accggctccg
     6841 ctggtgtcaa ccgtaattgt ggcaacgtcc ttcagcagcg cgacttcaac ggtaaaaacg
     6901 gctccggttt cagggagttc cgaaacgcca tgggctgcgc ggagcttttc cacgatagcc
     6961 ttcttgacta ttttctgaca ggccggcaca gacgtcagaa ccgatttgac actgcggccc
     7021 agcaccggaa acgcgccgtc agccggaatc agttcctgcc aatcgattgc gtaagtacgg
     7081 tcgaacagtt cgccgaaatc ggctgcctcg aactggccca tcatcagcag tacgcggtca
     7141 gccgtgcgta aatgcacgtt agccgtcgcc aacgtgcgaa tatcgccgtt gaaatgaatc
     7201 ctgccggact gcgttatccg cgacgctatc ccaagcgtct ccaattcgcg ggacaccagc
     7261 gattcaaggc cgaaagtgga agtcgccaca agttccatgt cattcatcgc cggctccgtt
     7321 gctggcattg ccaaacggca ctcttattac gtaaggccgt atgacgaata tcctgtcgta
     7381 atccatgccc tgcttggtgc aggtcagaaa atggttcgca acgctgtttg gccggctggc
     7441 ctggctgcca gggccgatga ccagaaaatc atccgtcggc attttcacgc gaaaggccat
     7501 ctcgtccagc ggatagaact ggcttttgta aactattctc ggatcgtcgt tatccgtttc
     7561 tatgccgaag tgccgaactg tactgcgtat ctgcggcacg gcgacgatta tgatgtcccg
     7621 atttgtttcc tcgtccagga acgtactcaa cgtcagcaga ttgtcgccag gcggaaagtg
     7681 ttcgccgcgc agggtttggg catcgctgaa aataaagatg ttctgaacgg gctgtccggt
     7741 tttcatttca agatgtgcgg gataacccaa tcgcgtttcg atgatttttt tctggaaact
     7801 caggccgccc tgtcgggcaa gaaggctttc cacctcagcc cagtcgcagt tgcggccaac
     7861 gcccacgcga aagccgttca tgcccagaac tgtggaatgc aggctgaccg gctcttcgtc
     7921 gagaaacgtc caaaattttt ccagcctgcc tgccgtgcct gccggcaatt ctatgctgtt
     7981 cacttccagg cgaatgacct tgaattcccg ccgcatccgc gtaggccttt gcggctggga
     8041 tttatcggga gcggcagatg aaattttagt tccggtatcc ggcatttttg agacagccga
     8101 cgtttctgcc ggcttgacag agttgtcctg gccggcagtt atgacgccga tattttcgca
     8161 tccgacaacc agcaggatat ttaatgttaa aaaaatttgc agcaggctac gacacatgtg
     8221 gtctatttta cctcatcgca tttcgtcgcc atattcacgg tggcagccgg ctgtgggccg
     8281 gtcagccgat aataaccgcg cgcaaatttg acgacaatca tcagccacgc cagaatcgct
     8341 atgcagggat acgcgatgaa tctcgtataa tacggtatca ggacggcaaa accagcgcct
     8401 gtcgcgatat cgcggcaatg cacgatatcg ccgaggttga acgtcgcccc gcatacgtgt
     8461 gacggccgca gcagttgctg ccacggcgta agcatggcca gcaaaacagc cgcccagcat
     8521 actgaactga taatctgcct tatgccgcgt accgccggcc caaccagcga aagatgtccc
     8581 gcgacagtct gaagcagaaa cagaaaaccg ccagccgcaa aagcaatgat tttcgtcgca
     8641 gccaacaccc aattcaatgc atactgccag ttaatcgtcc gctgccgcgt acattcggac
     8701 gtttttgtca taaacgacgt cgccggctca gtcgcacaag ccgccaccgc agccgccgca
     8761 gtttcagccg gcattgtagt aggcgtcgcg gacgtatgaa cggcggctgg aacggattta
     8821 accgtacac
//
LOCUS       DAONFI010000059         8639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9393_length_8639_cov_4.548695, whole genome shotgun sequence.
ACCESSION   DAONFI010000059 DAONFI010000000
VERSION     DAONFI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9393_length_8639_cov_4.548695"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLK08_03005"
     CDS             complement(<1..979)
                     /locus_tag="PLK08_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPS52499.1"
                     /translation="MMGYDFSTIESKWQKYWADNNTFVQPNPGQDGFDSSKPKYYVLD
                     MFPYPSGAGLHVGHPEGYTATDIVARYKRMKGFNVLHPMGYDSFGLPAEQYAVEHGVH
                     PRVTTEKNIENIERQIKMFGFSYDWSRKLATTDVNYYRWTQWIFLQLFNSYFDPAEKK
                     ALPVSVLKDRLMRDIYFVSPSGEIVYDVNHDMESIAGIPVGALKFSELPADEQRDFID
                     DQRLAYMSEVPVNWCPALGTVLANEEVTNEGKSERGNHPVFRRAMKQWMLRITAYAQR
                     LLDDIERVDWPEPIKIMQRNWIGKSSGAEVDFNVKGSDDIITVYTTRPDT"
     gene            complement(1097..2017)
                     /gene="ftsY"
                     /locus_tag="PLK08_03010"
     CDS             complement(1097..2017)
                     /gene="ftsY"
                     /locus_tag="PLK08_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPS52500.1"
                     /translation="MALFRKAVDRLLKGLKRTKAGVLGTLRGLLAGRQIDAALLEELE
                     MTLIQADIGVKSTMRIIDNLQTACKNGEIEQGEQVIEFLKSQLKQYWPAGDRAIKFAQ
                     NGPTVILVAGINGAGKTTSIAKLAYQFHSEGKKVLLAAADTFRAAAVEQLTIWADRIG
                     CDIVKGSGKKDPAAVVYEACEQAIAHNLDILLVDTAGRLHTQENLMRELTKIRDVVAK
                     KLPGAPHEVLLVLDATTGQNAINQARAFMKAIDATGIFLAKLDGTARGGIVVAIRDEI
                     NLPVKFVGVGEKFEDVELFDPEKFVNALFE"
     gene            complement(2032..2493)
                     /gene="nusB"
                     /locus_tag="PLK08_03015"
     CDS             complement(2032..2493)
                     /gene="nusB"
                     /locus_tag="PLK08_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPS52501.1"
                     /translation="MSSSQHRSRRLALQGLCCLDAQGMVAMPLVEHFIRDSHEAAGIV
                     KRAIELITEVMTDRDGCDALLARHARHWDLSRLALVDRNILRLGAHELRLCTLPYKIV
                     ISEAIRLAKEFSTAESPRFVNGVLDAVAREVCDVSSEDSGAEMAKADEADG"
     gene            complement(2554..3027)
                     /gene="ribE"
                     /locus_tag="PLK08_03020"
     CDS             complement(2554..3027)
                     /gene="ribE"
                     /locus_tag="PLK08_03020"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPS52502.1"
                     /translation="MKVYQGKLTAQAGVKFAIVVGRFNEFITNKLLGGALDALSRHGV
                     KEEQIEVVWAPGSFEIPVIAKHLAEGGRYDAVVCLGAVIRGGTDHYEYVAAEVAKGIG
                     QVGLSTGKPCIFGVLTCDTVEQAIDRAGAKSGNKGAEAALAALEMVNLLGELSGK"
     gene            3191..3985
                     /locus_tag="PLK08_03025"
     CDS             3191..3985
                     /locus_tag="PLK08_03025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HPS52503.1"
                     /translation="MSQNQPQPISQAAKRKPGPAVVHLPPEQMDYAHLVRKPINQLII
                     IAPLLVLFHVGSFFYGNRLLMPRYIAIALRWFDINWPGVVGIVIIFTLLCQQITSKTR
                     WHINVGTIFHGLLESAAWSLPLVAASWLTSLAYGEILPDSESPKAELCRKILEAVGAG
                     VYEEYVFRLLLIGLILLFFVDVAGFNRLKVSIAAIIFSAVLFSAAHFDEQQLLHLNTP
                     RIGKAIFLAVAGTLWGVLAVWRGYAMAACSHIWWNLLVIITTTTKF"
     gene            4066..5970
                     /gene="asnB"
                     /locus_tag="PLK08_03030"
     CDS             4066..5970
                     /gene="asnB"
                     /locus_tag="PLK08_03030"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPS52504.1"
                     /translation="MCGFAGEFVFKGQADLAVVERMAATLNHRGPDGSGHYTSVDGQC
                     AMAFRRLAVIDPENSGQPMTDSTGRYVLAFNGEIYNFRKLRSELQHDGIIFRTDGDTE
                     VFLQMLIKYGTGCAEKLRGMFAAVFYDSIEKKLSFLRDRMGQKPLFYAALPDRIIFAS
                     ELKALQKHPKINVNYNASFITSYLTIGYIPSPNTPFSTVKKFPPACRAEISASENFST
                     FSIFLKYYSAGENPSALPENSNELAGFVREKIINAVGERLVADVPLGLLLSGGIDSAV
                     VLAAMCEHLSATSIKTFTAGFENAAYDERPDAAALAKFFGTDHHELKISPAPEKMLDW
                     VIDTYDEPFADSSAWPTYHICRAARKYITVALAGDGGDEVFGGYDRYRAMYISQHIKP
                     FQYLALRLAGAAVNFIAPYNERNRLRRFARFTEILPYPFSVQYFMCRRLFGPADLLKL
                     LNQDFLASNEIFVDEPAEWFYDLFEQGKSLSHAVNAQYHDVATYLPDDLLVKADRASM
                     AASLELRSPMLDYELVDIGLALPVSEKLNCRRGKLILQKAFSDVLPKNLFRRQKRGFA
                     VPLADWLRNGLKEEMLLTLTDKAFLDLKIFNSAAIAGLVNEHVSGQADHSHRLWALLV
                     LAKWLIKPQS"
     gene            6145..7089
                     /locus_tag="PLK08_03035"
     CDS             6145..7089
                     /locus_tag="PLK08_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPS52505.1"
                     /translation="MEHKTAGSRLGRGLDALMGEADSFTAAETTHHGETQTEHKPQEI
                     HISLIAPNPNQPRQNFDEIQLEELAKSIRQEGILQPLVITESQNITSFKTDKPYVLIA
                     GERRLRAAHLAGLENVPVIFRNADDRKLIEWAMIENIQREELNPIERAHAYRDYMDRF
                     QLTQQEVAEKLGQARATVANFLRMIDLCDDVQTLVASGQVSFGHAKVIAGIAGNPELQ
                     AKLAHKVVSEGMSVRKLEEIIENSKENFSDENNRKKSPQKPAYVMDMEQQLTESVGTK
                     VQILPGRAKNSGKIILEYYSLDDFDKIAGLLGLQPEEI"
     gene            complement(7521..>8639)
                     /locus_tag="PLK08_03040"
     CDS             complement(7521..>8639)
                     /locus_tag="PLK08_03040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPS52506.1"
                     /translation="EDQSWGYCDTAGVPETRRFLADEVNKRGGAQVTPDDIIFFNGVG
                     DAVSKVYGFLRREARILGPTPAYSTHSSAEAAHSGYEHLTYELDPYNNWMPDIEDIRL
                     KVKYNDSITGILLINPDNPTGACYPREVLEEIADIARKNDLMIICDEIYAHITFNGHK
                     TLHLSEIIGDVCAIVMRGLSKEIPWPGSRCAWIEVLNRHKDETFNKYINSLLAAKRLE
                     VCSTTAPQMAVPRILGDKRYPEHLRQRAATIDARANEAVAILSKVPGTIVNKPCGALY
                     LTVMFEDGVLTDRQTLEINDPVVREEVERIVKNVPNDMRFVYYLLGATGICVVPLSGF
                     CCKRDGYRITLLECDDAKRKWTMETLTEATKKYLASTK"
BASE COUNT         2163 a         2163 c         2036 g         2277 t
ORIGIN      
        1 gcgtgtcagg ccgcgtggtg taaaccgtga ttatgtcgtc gctgcccttg acgttgaagt
       61 ccacttccgc gccgctgctt ttgcctatcc agttgcgctg catgattttt atcggctcgg
      121 gccaatcgac tctttcgatg tcatccaaca ggcgctgggc atacgcggtt atgcggagca
      181 tccactgttt catggcgcgg cggaataccg gatgattgcc gcgctcgctt ttgccttcgt
      241 tggtgacctc ttcgtttgcc aacaccgttc ctaatgccgg acaccagttt actggtactt
      301 ccgacatata tgccagtcgc tggtcgtcta tgaaatcgcg ctgttcatct gcaggcagtt
      361 cgctgaattt caatgcgccg acaggaatgc cggctatcga ttccatatcg tgattgacgt
      421 cgtaaactat ttcacctgac ggcgagacga aataaatatc gcgcatcagg cggtctttga
      481 gtaccgatac cggcagagct ttcttttcag ccgggtcgaa gtagctattg aaaagttgaa
      541 gaaaaatcca ttgcgtccag cgatagtaat tcacatccgt cgtcgcgagt tttctcgacc
      601 agtcgtaact gaagccgaac atttttatct gccgctcaat gttttcgata tttttctcag
      661 tcgtcacgcg cgggtgcaca ccgtgctcga cggcatattg ctccgccggc aggccgaagc
      721 tgtcataacc catcggatgc agaacattga accccttcat gcgtttgtag cgggctacga
      781 tatccgtcgc ggtataacct tcagggtggc caacgtgcag acccgcgccg gaaggatatg
      841 gaaacatgtc gagtacgtaa tatttgggtt tcgacgaatc aaacccatcc tggccgggat
      901 tcggctgaac aaacgtgtta ttgtcagccc agtatttctg ccacttactt tcaatcgtag
      961 aaaaatcgta acccatcatt aactccagaa atttcaattt gtcaattgca gattggaaat
     1021 tgaattacac tacacaccat taaacgcttg cttacaattt gccttttgca actttcaatt
     1081 tacaattcaa aaaatttcac tcaaacagcg catttacgaa tttttcgggg tcgaaaagtt
     1141 ctacgtcctc gaatttttcg ccgacgccga cgaattttac aggcaggttg atttcatcgc
     1201 gtattgccac gacgataccg cctctggccg tgccgtcgag tttcgcaaga aaaatgcccg
     1261 tggcatcgat agctttcata aacgcgcgtg cctgattgat ggcattctgg ccggttgtgg
     1321 cgtccagtac caacagaacc tcatgcggcg cgcctggaag tttttttgcg accacgtcgc
     1381 gtatcttggt aagttcgcgc atgagatttt cctgcgtatg cagacggccg gcagtgtcta
     1441 ccagcagaat atcgaggtta tgggcgatgg cctgttcgca ggcttcataa acgacggcgg
     1501 cagggtcttt tttccccgaa cccttgacga tatcacagcc aatgcggtca gcccatatcg
     1561 tcagctgttc gacggctgcg gctcgaaaag tatcagctgc cgcaagcagg acttttttac
     1621 cttccgaatg aaactgatac gcaagtttgg cgatggacgt tgttttgccc gcgccgttaa
     1681 tgccggcgac aagtatgacc gtcggcccat tttgtgcgaa ttttatcgca cggtcgccgg
     1741 ctggccaata ttgcttcaac tgcgatttga gaaattcgat tacctgttcg ccctgttcta
     1801 tttctccgtt tttacaggcc gtctgcagat tatctataat ccgcatagtt gattttacgc
     1861 cgatatccgc ctggattaac gtcatttcaa gttcttcaag cagggcggcg tctatttgtc
     1921 ggccggcaag caatccgcgc aatgtgccca gtacgcctgc ctttgtgcgc ttcagcccct
     1981 tgagtaatct gtccaccgct ttacgaaaca acgccatcct tgaaaatctc cttatccatc
     2041 ggcttcgtcg gctttagcca tttcagcacc agaatcttcc gaactcacgt cgcagacttc
     2101 gcgggcgacg gcatcgagta cgccgtttac gaaacgcggg ctttcggcag tggaaaattc
     2161 tttcgccagc cggatagcct ccgatattac aattttgtac ggcagcgtac aaagacgaag
     2221 ctcatgtgcg ccaaggcgca gaatatttct gtccaccagt gcgagccgcg aaaggtccca
     2281 atgcctggca tgacgcgcca gcagcgcatc gcagccgtcg cgatctgtca tcacttcagt
     2341 gataagttcg atagctcttt tgacaatacc cgcagcttca tggctgtcgc gtataaaatg
     2401 ttccacgagc ggcatggcaa ccatgccctg cgcatcaagg cagcacaagc cctgcaacgc
     2461 aagccttctg gaacgatgtt gagaactgct catttcaact cgcttttttg ttataacctt
     2521 gcaatcagac agacggccaa aacgtatcgc gggtcacttg cccgaaagtt cgcccagcag
     2581 gttcaccatt tccagcgccg ccagtgccgc ttccgcgccc ttgttgccgc tcttggcgcc
     2641 tgcgcggtcg atagcctgct cgaccgtatc gcacgtcaat acgccgaata tgcacggttt
     2701 gccggtagac aggccgactt gccctatgcc cttggcgact tccgcggcga cgtattcgta
     2761 atgatccgta ccgccgcgaa taacagcgcc gaggcatacg acggcgtcat atcggccgcc
     2821 ttccgccaga tgtttggcga taaccggaat ttcaaaactt ccaggcgccc agacaacttc
     2881 tatctgttct tctttcacgc cgtggcggct cagcgcgtcg agcgcgccgc cgagcagttt
     2941 gttggtgata aactcgttaa accggcctac gacaatggcg aatttcacgc cggcttgcgc
     3001 cgtcagtttg ccctgatata ctttcatgtc ggtttctcct gacaaatagg gacatcaagt
     3061 atatgcttta tcgacctgaa atggaatact ttcgtcagcc tgcagcagaa aatcttggcc
     3121 ttcaattttt caaatccaac tgtaaattag aattgctctg ttcagtcgat ttttgtaaat
     3181 tgcatcattg atgtctcaaa atcaaccaca acctatcagc caagccgcca aacgaaaacc
     3241 cggccctgcc gtcgtccacc ttccgcccga acagatggac tacgcgcatc tcgtgcgaaa
     3301 gccgataaat cagctaataa tcatcgcccc tctgctggtg ctgtttcatg tcggcagttt
     3361 tttttacggc aacaggcttc taatgccgcg atatatcgcc atcgcactgc gatggttcga
     3421 cataaactgg cccggcgtag tgggcatagt gataattttt acgctgctct gccagcagat
     3481 aacgtccaaa acccgttggc atataaacgt cggcactata tttcatggct tgctggaatc
     3541 tgcggcatgg tcgctgccgc tggtggcggc aagctggttg acgtcgctgg cttacggtga
     3601 aattctgccg gattcggaat cgccaaaggc tgaactctgc agaaaaatcc tggaagccgt
     3661 cggcgccggc gtttacgagg aatacgtttt ccgcctgctg ctgataggcc tgattttact
     3721 gtttttcgtc gacgtagctg gtttcaacag gctgaaagta tcgatagcgg caatcatatt
     3781 ttcagccgtc ctattctcgg ctgcacattt cgacgaacaa caactgctgc acttgaacac
     3841 gccgcgaatc ggcaaggcaa tatttctggc ggtcgctggc acattatggg gtgtgctggc
     3901 ggtgtggaga ggttatgcaa tggcagcctg cagccatata tggtggaatc tgctggtaat
     3961 cattacgacc acgacaaaat tttgacatat cgcccgattc tgcttcatac aaagttttca
     4021 tacttgtgca attagcttaa gtatttatta taaaagaagt taaaaatgtg tgggtttgct
     4081 ggagaatttg ttttcaaagg gcaggcggac ctggccgtcg tcgagaggat ggctgctacg
     4141 ctgaatcatc gcgggccgga cggttcggga cattatacca gtgtcgacgg gcaatgcgca
     4201 atggcgtttc ggcggctggc ggttatagac cctgaaaatt caggccagcc catgaccgat
     4261 tcgacaggcc gatatgtttt ggcgttcaac ggcgaaattt ataattttcg aaaactgcgg
     4321 tcagaactcc agcacgacgg gattattttc agaaccgacg gcgatactga agttttttta
     4381 caaatgctca tcaagtacgg aactggctgc gcggaaaaat tgcgcggcat gttcgctgct
     4441 gttttttacg atagcataga gaaaaaatta tcattcctcc gcgatcgcat gggccagaaa
     4501 ccgctatttt acgctgccct gcctgaccga atcatctttg cctcggaact caaagcctta
     4561 caaaaacatc caaaaataaa tgtcaactac aatgcttcgt ttataacatc ttatttaaca
     4621 ataggttaca ttccatcgcc caatactcct ttcagtacag tcaaaaaatt tccgccggct
     4681 tgccgagccg aaatttccgc atccgaaaat ttttcaactt tttcaatttt tttaaaatat
     4741 tattctgccg gtgaaaatcc atctgctctg cctgaaaatt caaacgagct tgccggattc
     4801 gtccgggaaa aaataattaa cgctgtcggc gaaaggcttg tggcagacgt cccgttaggg
     4861 ctgctgctca gcggcggaat tgattctgcg gtagtgttag ccgccatgtg cgaacatctg
     4921 tcagcgacat ccatcaagac gtttaccgca ggttttgaaa acgccgctta tgacgaacgg
     4981 cctgatgccg ctgccctggc aaaatttttc ggaacagacc accatgaatt aaaaatatcg
     5041 ccagccccag aaaaaatgct cgattgggtg attgatacgt acgatgagcc gtttgccgac
     5101 tcaagtgcat ggccaaccta ccacatctgc cgcgcggcaa ggaaatatat caccgtcgca
     5161 ctcgccggcg acggcggcga tgaagttttc ggcggatatg accgttatcg ggcgatgtat
     5221 atttcgcagc atatcaagcc gtttcaatac ctcgccctgc ggctcgccgg agctgcagta
     5281 aattttatcg caccgtataa tgagcgaaac cgcctcagaa gattcgccag gttcacggaa
     5341 attctgccct atccgttttc ggtgcaatat ttcatgtgcc gccgtctttt tgggcctgcc
     5401 gaccttctaa aactgcttaa ccaggatttt ctggcgagca acgaaatatt cgtcgacgaa
     5461 ccggcggaat ggttttatga cctgtttgaa cagggtaaat ctctcagtca tgccgtcaac
     5521 gcacaatatc atgacgtagc tacttatctg cctgacgatt tgctggtgaa ggctgacagg
     5581 gcttcgatgg cggcatcgct ggaattacgt tcgccgatgt tggattacga gttggtcgac
     5641 atcgggcttg cattgccggt tagcgagaag ttaaactgtc gccgtggtaa gctgatactc
     5701 caaaaagctt tttcagacgt tttgcccaaa aaccttttca ggcgtcaaaa aagggggttt
     5761 gccgttccgt tagcagattg gctgcgaaac gggttgaaag aagagatgtt attgacgctg
     5821 acggacaagg cttttcttga tttaaaaata ttcaattctg ccgctattgc ggggttggtg
     5881 aacgagcacg tttccggcca ggcagaccat agtcatcgtt tatgggcatt gctggtactg
     5941 gcaaaatggc taatcaaacc tcaatcctga ttctttttta cacctctacg ttcctgagtg
     6001 ttccacgtgg aacacagctc cttatcccat ccatcgccaa ataaatgttc cacgtggaac
     6061 atcccattcc gcttgacttg gggtgtagcc atatatagta tatcaatatc aaacttatac
     6121 aatttattgc aggagttcgc aatcatggaa cataaaacgg caggaagcag actcggcaga
     6181 ggcctcgatg cactaatggg tgaagccgat tcgttcacgg cggcagaaac aacccatcac
     6241 ggcgaaacac aaaccgagca taaaccacag gaaatccata taagcctcat cgcccctaat
     6301 cctaatcagc ccagacaaaa tttcgacgaa atacaactcg aagaacttgc aaaaagcatc
     6361 cgacaggaag gcatactcca accgctcgtc atcacggaaa gccaaaatat aacctcgttc
     6421 aaaacggata aaccttatgt cctgatagcc ggcgaacgac gcctgcgggc agctcacctg
     6481 gctggccttg aaaatgttcc cgtcattttc cgaaacgctg acgaccgtaa actcatcgaa
     6541 tgggctatga ttgaaaatat acagcgggaa gaactcaacc cgatagaacg cgcccacgct
     6601 tatcgcgact atatggacag atttcaactg acgcagcagg aagtcgccga aaaactgggg
     6661 caggctcgtg cgacagtggc caacttcctc cgcatgatag atctgtgtga tgacgtccag
     6721 acactggtcg cttccggaca ggtatccttc ggccacgcga aagttatcgc cggcatcgca
     6781 ggaaatccgg aattgcaggc gaaactggct cataaggtag tttcagaagg aatgtccgtc
     6841 agaaaactgg aagaaatcat agaaaacagc aaagaaaatt tttcagacga aaataaccgg
     6901 aaaaaatctc ctcaaaaacc tgcttatgtt atggatatgg aacagcagct taccgaaagc
     6961 gtcggtacga aagttcagat tctccctggc agggcgaaaa acagcggaaa aattattctt
     7021 gaatattaca gccttgatga tttcgacaaa atcgctggcc tgcttggact tcagccagaa
     7081 gaaatctgat aataccaaca ccgctttatt taaaattgca aaagtcaaaa ttctgaaatt
     7141 agttttatat gacataacaa aatctgttat caaaaagtta caattaagaa tccagatgcc
     7201 aatgattgaa tgcctgtctt ttactctaat aatatatata aatatagcag tagtattaaa
     7261 acagggggtg ttgaaagtgt tacggaagac ggctgataga tataactatt tttgacacaa
     7321 agacttataa tgacagagag ttgtctatca gtgtgaataa actgtaaata agttacgtgg
     7381 cagcctgttt atcagatacg gtttttaata ctgatttttt attgaacaga taaattagag
     7441 ttttatcgtg ggcaacataa aaattctcaa caaacaactg tcagcttatg cacatactgc
     7501 taaccaccat ttgttgaaaa ttatttcgtg cttgcgagat attttttggt agcttcggtt
     7561 aatgtttcca ttgtccattt acgcttggcg tcgtcacatt caaggagagt gatgcgataa
     7621 ccgtcgcgtt tgcaacaaaa tccgcttagc ggtactacgc aaatacctgt tgcgccgagg
     7681 agatagtaga cgaatcgcat gtcattcggc acatttttga cgatgcgttc gacttcttca
     7741 cggacgaccg gatcatttat ttccagagtc tggcggtctg taagtacgcc gtcttcgaac
     7801 atgacggtta gataaagtgc accgcagggt ttgttgacga ttgtaccggg gactttggac
     7861 agaatggcga ctgcttcatt cgcacgagca tctattgttg cagctctttg tctaagatgt
     7921 tcagggtatc gcttatctcc caaaattctc ggaacagcca tttgcggcgc ggttgtggag
     7981 cagacttcca gccgttttgc cgccagaagt gaattgatat atttgttgaa agtttcatct
     8041 ttatgtcggt tgaggacttc tatccacgcg catcttgaac cgggccatgg aatttccttg
     8101 ctcaggccgc gcatgacgat ggcacaaacg tctccgatga tttcacttaa atgcaaagtt
     8161 ttgtggccgt taaacgtgat atgcgcatat atttcatcgc atataatcat caaatcgttt
     8221 ttgcgggcga tatccgctat ttcttccaat acttcacgcg gatagcatgc tccggtagga
     8281 ttgtcgggat ttatgagcag gataccggtt atcgaatcgt tgtatttgac cttcaggcgt
     8341 atgtcttcga tgtccggcat ccagttgtta tacgggtcaa gttcatatgt aagatgttcg
     8401 taaccgctgt gtgccgcttc cgctgaagag tgtgtgctgt aggctggcgt agggccgaga
     8461 atgcgagctt ctcttctcaa aaaaccgtaa acttttgata ctgcgtcgcc gacaccgttg
     8521 aagaaaatta tgtcgtcggg cgtgacctgg gcgccgccgc gcttgttgac ttcgtctgct
     8581 aaaaaccgtc tggtctctgg cacaccggca gtatcgcaat aaccccagga ctggtcttc
//
LOCUS       DAONFI010000060         8631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9410_length_8631_cov_4.321129, whole genome shotgun sequence.
ACCESSION   DAONFI010000060 DAONFI010000000
VERSION     DAONFI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9410_length_8631_cov_4.321129"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLK08_03045"
     CDS             complement(<1..237)
                     /locus_tag="PLK08_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPS52507.1"
                     /translation="MAEVNVMEQIVSLCKRRGFVFPASEIYGGINGFWDYGPLGVQLK
                     NNIRDWWWRCMVECPPIGPDGHPVQIVGLDSAIIQ"
     gene            complement(510..1709)
                     /locus_tag="PLK08_03050"
     CDS             complement(510..1709)
                     /locus_tag="PLK08_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPS52508.1"
                     /translation="MKVFYCTVMNPRPVQLDIYRLSIPMRGFEHAAARRKTAESVVVA
                     MRYSDGYIGWGETLPRAYVTGETMETVPADIETLFKRCCDDGILDVAAGKIPQKVPTA
                     VNNRCVNAAACALDLAGLRRFFADFNTVDPKILMALTNRARVRNYIDSHVSGVLGSAN
                     PQKTAWRLRIMKFADIKDFKLKLGLGEEVDRQNLDAVYSQLRRGLQKGTHSLRVDING
                     GWKKEDVPQKVAQLRKYGVCAVEQPCYCSAAELVEISRQCKVPLMADESLLTLDDART
                     LLAEPEKIWWNVRLSKNGGLLPVLELLELAQKSNVVVSLGCMVGETAILSAAQRRVLQ
                     LGPVPKFLEGNYGRYLLDDDIIPGRRSLMFGWAGSLRVLKGDGLGVTPCPEKLKKYAK
                     KLKTLTA"
     gene            1851..2903
                     /locus_tag="PLK08_03055"
     CDS             1851..2903
                     /locus_tag="PLK08_03055"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPS52509.1"
                     /translation="MSVERDVIGTLPDGKPVEKLTITNSGGMKIAAMNYGATLIRVEV
                     PDRYGKMADVVLGFDNLRDYVEHNQFFGSTVGRYANRIACGTFTLDGVDYQLAVNNNG
                     NHLHGGDIGFDKKSWAAQVSHHDNAVRFTYVSPDGEENYPGTLECNVTYSLGEDNDLR
                     ITYHAVTDKPTILNLTNHSYFNLAGRGNILNHMLSINAHRFTPTDENQIPTGEIRPVA
                     GTPMDFVNLHRIGDRMDANDEQLLIGGGYDHNWVLNKTIAGELSLAARVEEYDTGRCL
                     ECHTTTPGIQLCTSNFLNLDVPGKKGMPLTRYAAFCLETQYFPDSPNKPNFPSAVLRP
                     GDIYKQVTVYKFGTVE"
     gene            complement(2914..3813)
                     /locus_tag="PLK08_03060"
     CDS             complement(2914..3813)
                     /locus_tag="PLK08_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52510.1"
                     /translation="MNIAVVRCKPNPPWILSYVENDMKFTQIGRAAIFGLVVAAVAVF
                     AAGCKEKVHHHHAEPTPPPRPCPVPPQPQPSPMQMWTEISGRVFIDAKPCKDATITVF
                     WGKGRCYKKSCQTNKKGDFVICEIPPKREIAIEFRGRHHEREYFSARSMWTGRAGDIM
                     VLGRIDLSEVRPQPRPQPMPGPVPGPKPAPHPKPTPGPMPHPEPVSRCDLKISGCVMF
                     EGRPAQNAAVRITSGRIYERTVKTDDRGRFRVADVPRHARIEIFAEQKKHHLVLVGSA
                     VIQTGDNNVDIGPLTLFTKKKKK"
     gene            3926..4567
                     /locus_tag="PLK08_03065"
     CDS             3926..4567
                     /locus_tag="PLK08_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPS52511.1"
                     /translation="MRKHVKKMMTGLFIVGPLGATVWIIVIVGQFVGRLGRGVFGITQ
                     LSVESKWEPVASGLGVLAVLLLIYLVGVLADFWMFSRFFNWVDQVLSSLPGIKTVYES
                     VRDLMKLFGQTSEKRRGRPVLFKPEGSSFAMLGIVTNEKPKVCVNGEKRVVVFLPMGY
                     MIGGPTVLARPCDLTPLDMSVETAMKLAITAHITDEDSTVRKIENPDEAENSF"
     gene            4810..6669
                     /locus_tag="PLK08_03070"
     CDS             4810..6669
                     /locus_tag="PLK08_03070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HPS52512.1"
                     /translation="MAKKAPNTKTSKKDATGFLFDDNQVTAESSTVAEKPAKAKRISK
                     KTPPSNPQLPEETEEHSPVTKRKSAKQKYATAESMAKAQRSISVSEFFAKNRHLLGFD
                     NPRKALLTTVKEAVDNSLDACEEAGIMPLVEVAIDQISETHFRVMVQDNGPGIVRQQI
                     PNIFGRLLYGSKFHRLRMSRGQQGIGISAAGMYGLLTTGKPVKIISRTGKRSPAHSYT
                     LKIDTRNNKPEILADEIVDWAPEHGTCVEIELEARYQKGRQSVDEYLQQTAVANPHVT
                     FKYKTPDGRDISYTASVMELPAIPKEIKPHPHGVELGVFIKMLQDTTAKNLQQFFTSE
                     FSRISPKLADEIIKKAGMGLTPKTRPAGVDGDKARALHVAINDVKILAPNTDCIVPIG
                     HEQLIAGLKQVVDAEFYAAISRKPAVYRGNPFVIEAAIAYGKNPACQSQQQQTLLDET
                     ENDDDNDDATSADDDNGGDSLARVLRLANRVPLQYQQGACAIYKSVAQMSWRKYGLSQ
                     SRGALPAGDLTIVIHMGSVWVPFTSESKEAIASYPDILKEIRLALQECGRKLGAHIRK
                     TSRIRQELKKRSYIEKYIPAIGQALKDILNLKDRQVEKVCDELKDILEKSRKM"
     gene            complement(6701..7039)
                     /locus_tag="PLK08_03075"
     CDS             complement(6701..7039)
                     /locus_tag="PLK08_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS52513.1"
                     /translation="MDLNVEKKDGVAIVTLLGSHLDASNHELFMQDITPVIEENPNLL
                     LDIGELQALDSSGLGAFAYCMRTARDAGGRLTICRPSPRVLVTFELVHIDRIIGVYDT
                     RDAAVDSYKK"
     gene            complement(7330..8553)
                     /locus_tag="PLK08_03080"
     CDS             complement(7330..8553)
                     /locus_tag="PLK08_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPS52514.1"
                     /translation="MAKFKYEIQKANQQIVTGIVEGASIMEASAKVRQLGGYLVKIEP
                     AMSAASVIDKMRNIRIESLPSHKDIMGLTKQLAVMVRAGISIRDALEGIAEQQKNLRF
                     RDILRQCRADVESGNQFSDAIAKNPKVFSLMYVNMVRASEMSGTFAKMLDRIGKYLEE
                     QHETRSMVRGAMIYPAVLFCMSVGAVIFLLTWVLPRFMGVFRGKEQLLPKPTKMLMAM
                     SNFMIAHWPIILVGAVVFIVFIIALNKTKKGRRFLDLLKLKVPILSKMFRAIYIARSL
                     QTMGELVLAGVPMLEVLQITSDIAGNGYYYDMWQKVKNSVKEGNKIVTELNNSPLLPQ
                     SVVQMIGSGEDSGRLGEVLEDVSEFYAKELRDTIKAVTALLEPLMIVFMGFVVGFIAM
                     SIILPVFKMSSLAGK"
BASE COUNT         2239 a         2233 c         2079 g         2080 t
ORIGIN      
        1 ctgaattatc gcgctgtcaa ggccgacaat ctgcactgga tggccgtcag ggccgatggg
       61 cggacactcg accatacacc gccaccacca gtcgcggata ttgtttttga gctgtacgcc
      121 caacgggccg taatcccaaa aaccgttaat cccgccgtaa atctcggacg cgggaaacac
      181 gaaaccacgc cgcttgcaca gcgacactat ctgctccata acgttaactt cagccattat
      241 ttatgtccat attcgtataa aattttcaag aatcaaaaaa caagtttcag gcaatccaac
      301 ttacaattat ctatcaaaat ttccaaaaaa cgttttctgc gattctttaa acatgcaact
      361 ttggaattgc ctgtttgtca cttgtaactt ttgcggaaac gggaattgta gccaaaaacc
      421 gccgaaaaac aaggtttttt aaacatccgg cagatttatg tgacgatggg agctgattgt
      481 ttttatgata tgcttttcaa tttatgcatt tatgcagtca atgttttgag tttttttgcg
      541 tattttttga gtttttctgg acagggagta acgccaaggc cgtcgccttt aagaactcgc
      601 agcgaaccgg cccaaccgaa catcagactg cggcggccag ggataatatc atcgtcgagc
      661 aggtagcggc cataattacc ctccaaaaat ttcggcaccg gcccaagctg aagcacccta
      721 cgctgagcag ccgacagaat cgccgtctcg cctaccatac agccaagcga caccacgaca
      781 ttgcttttct gcgccaactc aagcagttcc agtaccggca gcaggccgcc gttcttgctc
      841 agccgcacat tccaccagat tttctccggc tcagccagca gggttctggc gtcatcaagc
      901 gtcagcagcg actcgtccgc catcaggggc actttacatt gtcgtgaaat ttccaccagt
      961 tcggcggcgg agcagtagca cggctgttcg acggcacaga cgccgtattt gcggagttgt
     1021 gcgaccttct gcggcacgtc ttcctttttc catccgccgt tgatatccac tctcaacgag
     1081 tgcgtgcctt tttgcaagcc tcgccggagc tgcgaataaa ccgcgtcaag attctgccgg
     1141 tcaacttctt cacccaggcc cagcttgagt ttgaaatcct tgatatcggc gaatttcata
     1201 atcctcagac gccaggctgt tttttgtgga ttggcacttc ccaatacgcc agatacgtgc
     1261 gaatcgatat aattcctcac gcgggcgcga ttggtcaatg ccatcagaat tttcgggtct
     1321 accgtattga aatcggcaaa aaaccgccga aggcctgcaa ggtcgagggc acatgcagcg
     1381 gcgttgacgc aacgattatt gacggcggtt ggcacttttt gggggatttt gccggcggct
     1441 acgtccaata tgccatcgtc gcaacagcgt ttgaaaagcg tctcaatatc ggcgggaact
     1501 gtttccatgg tttcgccggt cacgtaagcc ctcggcagag tttctcccca gccgatgtag
     1561 ccgtcactgt aacgcatggc tacaacaacg gattccgccg tcttacgacg tgcggctgcg
     1621 tgctcgaaac cgcgcatggg tatcgacagg cgatatatgt ctaattgtac cggccgtgga
     1681 ttcatgacag tgcaataaaa tactttcatt tacccgtcaa ttccagtatt tttaataaaa
     1741 gaacagccta caattctctc acagccttgc tggtgctcgt cgccaacacg cgaaaatacg
     1801 gctgcgattt tggaagctgt ttaaaatccg cttcagcatg aggaactttt atgagcgtgg
     1861 aacgtgacgt aatcggcact ctgcccgacg ggaaaccggt ggaaaagttg accataacca
     1921 acagcggcgg catgaaaatc gccgctatga attatggcgc gactcttatc agggtggaag
     1981 tgcccgaccg ctacggcaaa atggctgacg tggtgctggg gttcgataat ctcagagatt
     2041 acgtggaaca taaccagttc ttcgggtcaa ccgtgggacg atatgccaac agaatcgcct
     2101 gcggaacgtt cacgctcgac ggcgtcgatt atcagctcgc agtaaacaac aacggcaatc
     2161 atctgcacgg cggggatatc gggttcgaca aaaaatcctg ggccgcacaa gtcagccatc
     2221 acgataatgc cgtgcgtttt acgtacgtca gccccgacgg cgaggaaaat tatccgggca
     2281 ccctggagtg caatgtcacc tattcgctcg gcgaagataa tgacctgcga atcacatatc
     2341 atgccgtgac ggacaaacca accatactga atcttaccaa tcacagttac ttcaatcttg
     2401 ccggccgtgg caatatcctg aaccacatgc tttcaataaa tgcccatcgc ttcactccga
     2461 ccgacgaaaa ccagatacca accggtgaaa tcaggcctgt cgccggcacg ccgatggatt
     2521 tcgtcaacct gcatcggatt ggcgacagga tggatgccaa tgacgagcag ttgctgatag
     2581 gcggcggata tgatcataac tgggtgctca acaaaaccat agccggcgaa ttaagcctcg
     2641 ccgcacgtgt tgaagaatat gatacgggac gatgtctgga atgtcacaca accactccag
     2701 gcatacaact gtgcacgagc aattttttaa acctggacgt gccgggcaaa aaaggtatgc
     2761 cgctgacccg ttacgcggca ttttgccttg aaacgcaata ttttcccgat tcgcccaaca
     2821 aacctaattt tccgtctgcc gtccttcggc ctggcgatat ttacaagcag gtaacggtat
     2881 ataaattcgg aacggtcgaa tagaggtcgt tttttatttt ttcttctttt ttgtaaaaag
     2941 cgtcaacggc cctatatcga cattgttgtc gccggtctgt atcacagccg acccgaccag
     3001 taccagatga tgttttttct gctctgcgaa tatctcaatt cttgcatgcc gtggtacatc
     3061 tgctacgcga aatcggcctc tgtcgtcggt tttgacggtg cgttcgtaaa ttctgccgga
     3121 cgttattcgt accgcggcat tttgagccgg acggccttca aacatgacgc aaccgcttat
     3181 tttcaggtca cacctgctga ctggttctgg atgcggcatg gggcctggcg tgggcttggg
     3241 atgcggagcc ggtttggggc ctggtacagg gccgggcata ggttgcggcc ttggctgtgg
     3301 ccgaacttcg gaaaggtcta ttctgcccag taccataata tcgccggcac ggccggtcca
     3361 catcgacctg gctgaaaaat attcccgctc gtgatgcctg ccacggaact caatcgcaat
     3421 ttcccgtttg ggcggaattt cacatattac gaaatcgcct tttttgttcg tctggcagga
     3481 ttttttgtaa cagcggcctt tgccccagaa tacggtaatt gtggcgtctt tgcacggctt
     3541 ggcgtctatg aaaactctgc ccgatatttc cgtccacatc tgcattggac tcggttgcgg
     3601 ctgcggcggg acagggcaag gcctcggcgg cggcgtaggc tctgcatgat gatggtgaac
     3661 cttctctttg catccagcgg caaaaactgc caccgctgct acgactaatc caaaaattgc
     3721 cgctcggccg atttgcgtaa atttcatgtc gttctccaca taagataata tccacggcgg
     3781 attcggctta catcgcacaa ccgcaatatt catatcgcaa aatcattttt atccattata
     3841 gtaagctcag ccgtacaaaa ccattattcc gttgaatttc cgccggaatt ctgtcaaaat
     3901 gtcgcgagtc gaaaggcaat gaaaaatgcg taaacatgta aagaaaatga tgactggcct
     3961 ttttatcgtc ggccctcttg gtgcgaccgt ctggattata gtgatagtcg gccagttcgt
     4021 cggcagactg ggccgaggcg ttttcggaat aacccaactg tcggtcgaaa gcaaatggga
     4081 acctgtcgcc agcggcctgg gcgtactggc tgtgctgctg cttatttatc tggttggagt
     4141 gctggcggat ttctggatgt tcagccgatt tttcaactgg gtagaccagg tgctaagctc
     4201 tctgccgggc ataaagacgg tatacgaatc ggtacgagac ctgatgaaac ttttcggcca
     4261 gacgtcagaa aaaagacgtg gccggccggt tctgttcaag ccggaaggct cgtctttcgc
     4321 catgctcggc atagttacga acgaaaagcc caaagtatgc gtgaacggcg aaaaacgcgt
     4381 agtcgtcttt ctcccgatgg gatacatgat aggcgggccg acggtactgg cacggccgtg
     4441 cgacttgacg ccgctggata tgtctgtcga gacagccatg aagctggcaa ttacggctca
     4501 tatcaccgat gaagattcaa ccgtaagaaa aatcgaaaat ccggatgaag ccgaaaacag
     4561 tttttaatat gacggtcgta attttccggt ttttttaaaa aacatagata aaaatatccc
     4621 ccgacaagca gataaacgaa agcctgacaa atatgattct aaacggtttt aacgcgtaaa
     4681 aaattttgat atgcctgtcg taattttccg tttttttaaa aaagcataga taaaaatgtc
     4741 caccgataga cggacaaatg aaaatcttat aaatacgttg tacgaatata cggaccgaag
     4801 gagccacgga tggccaaaaa agcccccaac acaaaaacct caaaaaaaga cgcaacagga
     4861 tttcttttcg acgacaacca ggtgaccgcc gaatcgtcaa ctgtggcgga aaaacctgct
     4921 aaagccaaac ggataagcaa aaaaacacca ccctccaatc ctcaacttcc ggaagaaacg
     4981 gaagaacact cgcctgtaac gaaaagaaaa tcggcgaagc agaaatatgc tacggcggaa
     5041 tccatggcca aggctcaacg gagtatttcg gtaagcgaat ttttcgccaa gaaccgccat
     5101 ctgctgggat tcgacaatcc cagaaaggct ttgctgacca cggtaaaaga agcggttgac
     5161 aactcgctgg atgcttgcga agaagccggc ataatgcccc tggtagaagt cgccattgac
     5221 cagatttccg agacgcattt tcgggtcatg gtacaggata acggccctgg catcgtcagg
     5281 cagcagatac ccaatatctt cggcagactg ctttacggga gtaaatttca caggctgagg
     5341 atgagcaggg gccagcaggg tatcggcata tccgcagccg gcatgtatgg ccttttgact
     5401 acgggcaagc cggtcaaaat catcagccgc acaggcaaac gctcgccggc tcattcatat
     5461 acgcttaaaa tcgacacgcg aaataacaaa ccggaaatcc tcgcggatga aatcgtcgac
     5521 tgggctccgg aacacggcac ttgcgtggag atagagctgg aagcccgcta tcagaaaggc
     5581 cgccagagcg tagatgaata tctccagcag acggccgtcg caaatcctca tgttacattc
     5641 aaatataaaa cgccggacgg acgcgatatt tcctacaccg cctcggtcat ggaactgccc
     5701 gcaatcccca aggaaatcaa gccccatccg catggagtgg aactgggcgt tttcataaaa
     5761 atgctccagg atacgactgc taaaaatctg cagcagtttt tcacgagcga attttcccgc
     5821 atctcgccca agctcgccga cgagataatc aaaaaagcag gcatgggtct aactccaaaa
     5881 acacggccag cgggcgtcga cggcgataag gccagagcac tccacgtcgc aataaacgac
     5941 gtcaaaatac tcgcgccgaa tacggactgc atcgtgccca tcggccacga acagttgatt
     6001 gccggattaa aacaggtggt cgacgcggaa ttttacgccg ccatctcgcg caagccggcg
     6061 gtctatcgcg gcaatccgtt cgtcatagaa gcggctatcg catacggcaa aaatccagcc
     6121 tgccagtcgc aacagcagca aaccctgctt gatgaaaccg aaaatgacga cgataatgat
     6181 gacgcgacgt cggccgatga cgacaatggc ggagattctc tggcgcgagt gctccgcctc
     6241 gccaatcgtg tgccgctgca gtatcagcag ggagcatgcg cgatttacaa aagcgtggca
     6301 cagatgagct ggcgcaagta tggcctctca cagagccgcg gtgccctgcc ggctggcgat
     6361 ttgaccatag tcatacatat gggcagcgtg tgggtcccct ttacgagcga atcaaaagag
     6421 gctatcgcat cgtatcccga tattttgaag gaaataagac tcgctctcca ggaatgcggc
     6481 agaaaactgg gagcgcatat tcgtaaaacg tcccgaatca ggcaggaact caaaaaacgc
     6541 agctacatag agaaatacat cccggccatc ggccaggcat tgaaagacat actcaacctt
     6601 aaagacagac aggtcgaaaa agtctgcgac gaattgaaag atattttgga aaaaagcaga
     6661 aaaatgtaat ttttcaaatt caaacgggag cttacgccgg ttattttttg tatgaatcca
     6721 cggcggcatc ccgcgtgtcg tatacgccga tgattctgtc tatgtgcacc agttcgaatg
     6781 taaccagaac ccgcggcgaa ggacgacata tcgtcaatct gccgccggcg tcgcgcgccg
     6841 ttcgcataca atacgcaaac gcccccaggc cggaactgtc caatgcctgt aattctccga
     6901 tatcgagcag cagattagga ttttcttcga tgacgggtgt tatgtcctgc atgaacaatt
     6961 catgattgct ggcgtcaaga tgacttccca aaagagtcac tatcgccact ccgtcctttt
     7021 tttctacgtt caaatccata ttctgaattc cttcctttac cttcgctaga gcagttcttg
     7081 aaagtttttt cttttttgac gccagctaaa ggttttctga caaggacgtc gaagcaggca
     7141 accttatatg gtttccgatg cagacgggcg tagtcgaaaa accttacagc aaggccaaat
     7201 ggaacatttt ttcaagaact gccttaagta ttacatataa tccggcaata tgcaacagat
     7261 tcctgctcaa aatcaggcgt caatacgggg cgttaatctt tgcgaaaata atcggttatg
     7321 gggcttttgt catttgccgg ccaggctaga catcttgaat accggcagga tgatgctcat
     7381 ggcgataaag cctacgacaa agcccataaa aactatcatc aacggttcaa gcagtgcggt
     7441 aaccgccttg attgtatctc tgagttcttt tgcgtaaaat tcggaaacgt cttccagaac
     7501 ttcacccaga cggccggaat cctcgcctga gcctatcatc tgtacgacac tctgcggcag
     7561 caacggactg ttgttaagtt cggtgactat tttattgccc tcctttaccg agtttttaac
     7621 tttctgccac atgtcgtagt aatacccgtt tcccgcaatg tcggatgtta tctgaagtac
     7681 ttccagcatc ggtacgccgg ctaaaaccag ttcacccata gtctgaaggc tgcgggcgat
     7741 ataaatagca cggaacattt tggacagaat gggtactttg agcttgagaa gatccagaaa
     7801 tcgtcttcct tttttcgttt tgttgagggc tattataaat acgataaata cgactgcgcc
     7861 gaccagtatt atcggccaat gtgcaatcat gaagttgctc atcgccatga gcattttagt
     7921 gggcttcggc aaaagctgct ccttgcccct gaatacaccc ataaatcgcg gcagaaccca
     7981 cgtaagcaga aagatgactg cgccgacgga catacaaaag agaaccgccg gatatatcat
     8041 cgcgcctcgc accatacttc tcgtttcgtg ctgttcttcg agatattttc ctatgcggtc
     8101 gagcattttt gcaaaagtgc cggacatttc ggatgcgcgc accatgttca cgtacatgag
     8161 cgagaaaact ttggggtttt ttgcgattgc atctgaaaac tggttgccgg attccacatc
     8221 agcccggcat tgccggagta tgtcgcgaaa tcgcagattt ttctgctgct cggcgatgcc
     8281 ttccagtgca tctcgtatac ttatgcctgc gcgaaccatg accgccagct gtttcgtcag
     8341 acccattatg tccttgtggc tgggaagcga ctctatgcga atattgcgca ttttgtcgat
     8401 tacgctggcg gcactcatcg ccggctcgat ttttacaaga tacccgccca actgacgaac
     8461 ctttgccgac gcctccataa tggaggcgcc ttcaactata ccggtgacta tctgctggtt
     8521 ggctttctga atttcgtatt tgaatttggc catgttcttt tacagctttt ttcttacacc
     8581 agccccagag cctgcttgag tgtttcagga tgattcgcct tcgtaagtgc t
//
LOCUS       DAONFI010000061         8614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9439_length_8614_cov_4.356350, whole genome shotgun sequence.
ACCESSION   DAONFI010000061 DAONFI010000000
VERSION     DAONFI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9439_length_8614_cov_4.356350"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLK08_03085"
     CDS             complement(<1..389)
                     /locus_tag="PLK08_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52515.1"
                     /translation="MRLSLKDLQYLVSGAADEFLTADLPADPLSLQKALRKCVGPDEA
                     MAIDQLRRVRKKAAAKMPLPQAEKMLADDTMIQQASSLAIAAYKGQKILQRWPDVRHV
                     LDLCCGCGLDAIGLAKCGLTVDGYDESS"
     gene            complement(474..1460)
                     /locus_tag="PLK08_03090"
     CDS             complement(474..1460)
                     /locus_tag="PLK08_03090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPS52516.1"
                     /translation="METSKKITYSIVVPFYNEQDIIEKLHDRLVKVMDSVGGEYELVY
                     VNDGSRDDTPAILEKIATANPRVVFVDLRRNFGQTTGLMAGFDVAGGEIIISMDGDLQ
                     HDPYEIPMFIEKIREGYDIASGWRIRRGDNFVMRRLPSKIANRLLAAVSGVALHDFGT
                     TFKAYRREVLEDVRLYGDMHRFVPAVCQRLGARICEVPIKNVRRAAGKSNYGIGRTFR
                     VALDIITLKFITGYLTRPMHFFGRWGMASFAFGLLILFYGFLRKIFAWNEFRLFEEHG
                     PLMALGFLLLIVGMFFLSTGLIGEMLMRIYFESTNARTYAVKRIFRNARQEK"
     gene            complement(1447..2307)
                     /locus_tag="PLK08_03095"
     CDS             complement(1447..2307)
                     /locus_tag="PLK08_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HPS52517.1"
                     /translation="METGKKCKVIINGDDFGLSRRVSEGIIHAHKYGILTSATFQANG
                     DASEYAASLLAEVPTLGVGVHLNISQGRCISKAGMKLADESGMMNHTAVGIIMLLLKK
                     PNLLSAVREEFDAQIRRAVAMGIRPTHLDTHRHSHGFGPVCSIVAELAKKYNIPFIRR
                     LGEHLPWVGWPKAPAKQKHISNILTFLGDMNKLRHWRFFRTAGTWGVAHTGCITREWL
                     LTAAGALVKVCPCKTIEIMTHPGYCDDLDESVTRLIESRQIELAALCDDDVRKAFDAN
                     GIERINYGNL"
     gene            2432..2764
                     /locus_tag="PLK08_03100"
     CDS             2432..2764
                     /locus_tag="PLK08_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008031506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HPS52518.1"
                     /translation="MFGKIFLKLSAADIWMAVGFFGQVVFGLRFIVQWIATERAKKSI
                     IPLAFWYLSLVGTLILLAYSIYRKDPVFIAGFSLNLIIYVRNLYFIHIHPRKCAAAKK
                     EIAANDEQ"
     gene            2751..4421
                     /locus_tag="PLK08_03105"
     CDS             2751..4421
                     /locus_tag="PLK08_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPS52519.1"
                     /translation="MTSNNDLYSDRRNILLLILLCVLLYVPGISSFPLCEPEEARCAL
                     TTSEMLQSGNWLMPTLCGKNYYNKPAPYYWLTAGVQWLTDDSEFAGRLVSAIFAAFAV
                     VLAYKLVRKFERKSVAMLAGIMLATCMEFWIMGRYYRMDMPFVTSMWAAMYYFASRET
                     CAVVDRRAGNSCWRGFYFWAAVATLFKGPAGLALPVMVVGTYLLATRQFRRILEFFNP
                     AGMAIYLLVACPWFIATSIKYPEYFGAFFGRENFGRVTGGGDLGHSWPGILYIPILFA
                     GCLPWCVYIPGAFIRYVPMFFGKTGREKIIVFLMTATVMPLLFFMFSHTKLVSYILPV
                     FPPLMVMLGVFVGRWSASSQPDKLMRIGAWTLMEFVAVMTVVIVGFEWKFRWLDLSAV
                     VPCVIVFYAIFRMARFIRTERRVRFIHWSAGCVVMLQLYMLVHIVPSGLEFLSSRREA
                     QLLPQAATNVCLYPDENLSFMFYTGHYTAPVISKRDTAAFENLTAELNSSRPIWCFVS
                     GQKYLKKLQQAVPAVKIIDSRNDNFLVTNSSGTTLSQNAVQPLKNVEK"
     gene            4573..7077
                     /gene="gyrB"
                     /locus_tag="PLK08_03110"
     CDS             4573..7077
                     /gene="gyrB"
                     /locus_tag="PLK08_03110"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPS52520.1"
                     /translation="MEQAEEVTNAQKKATVEYGENQIKVLKGLEAVRKRPGMYIGDTT
                     SRGLHHLVWEIVDNSIDEAMAGRCSSILVTINLDGSVSVTDDGIGIPVGIHPTEQIST
                     LEVVFCKLHAGGKFEEGAYKVSGGLHGVGASVVNALSEWLEADVCRDGDVYQMTFSRG
                     VKTQELTKIGKRKKTGTKVTFKPDSQIFPDTEFRYEILASRLKELAYLNEGLNIKLHD
                     ERTGREDSFCYSQGLLAFVKNLNEDKITFHKPIRITRLDEAAKMSCDIVIQYNDSYTE
                     NVFSFANNINTHEGGTHMSGFRSALTRTMNAYAKNNSLMKNVKTVPTGDDLREGLTAV
                     VSVKLTNPQFEGQTKTKLGNSEMETFITTTVNELLGAWLEENPTEAKKIINKAISALQ
                     AREAARKARDLTRRKGALSSGSLPGKLADCRSRDRDSTEVFIVEGDSAGGSAKQGRNS
                     NTQAILPLRGKILNIEKARLDKILSSNEIQVIISALGCGIGEDEFDLDKRRYNKVIIM
                     TDADVDGSHIRTLLLTFFFRHMKPLLTQGHIFIAQPPLYLVTRKRHKEYVINEKQMQT
                     TLLNLGLEGTTLEVHDTAQTPAVVVSSFKGAKLREVVELLDSLNDNIHILERRGITFP
                     MLLQHRGPDGKLPTHWMVMDSRNLFFASAEKYAEFLQKHADKLAATEDNENGHVESDE
                     NMPVHEVRKRAELHEVREMEKTIEKLAAKELFIDDYLIKREESVTGDLAPAKYVLVTE
                     EESLELNNISEIASGVRTLGGRGIETKRFKGLGEMNAEQLWETTMDPERRILRRVKAE
                     EAEDAERMFSLLMGDNVERRRQFIEDNALTVKNLDV"
     gene            complement(7169..>8614)
                     /gene="groL"
                     /locus_tag="PLK08_03115"
     CDS             complement(7169..>8614)
                     /gene="groL"
                     /locus_tag="PLK08_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPS52521.1"
                     /translation="PNENMGAQMVKEVASKTSDIAGDGTTTATIYAEAIFEEGLKNVT
                     AGANSMELKRGIDKAVAAIIDELGTLSNPVKSSKEVAQVGACSANQDAEIGKMIADAM
                     DKVGKDGVITVEEGKSLDTYVDLVEGMQFDKGYISPHFVTDMAAMECKLEKPLILIYE
                     KKLTAIKDLVPLLEKIAQAGRPLVIIAEDVEGEALATLVVNRLRGTFQCVAVKAPGFG
                     DRRKEMLRDIATLTGGKAIMEDMGISLENIQMTDLGTAKEIRVAKDTTTIIEGAGKES
                     DIRGRIEQIKTQIETTTSDYDREKLEERLAKLAGGVARINVGSATEVEMKEKKARVED
                     ALHACRAAVEEGILPGGGTAVLAAVKVLDKVKTSNEDEKTGVDIIRRALSAPIKQIAA
                     NSGLDGSVVAMKVMENPEKIANFGYNALTGKYCDLVKDGVIVPTKVERSALQNAASVA
                     GLLLTTDAVISDLPEKKDKPAMPGGGMDDMM"
BASE COUNT         2037 a         2201 c         2221 g         2155 t
ORIGIN      
        1 gacgattcat catagccatc gacagtcaat ccgcacttcg ccaaccctat tgcgtccaag
       61 ccgcagccgc agcataaatc cagcacatgc cgcacgtcag gccatcgctg gagtattttt
      121 tggcccttat acgccgcaat ggctagcgat gacgcctgct gaatcatggt gtcatcggcg
      181 agcatttttt cggcttgtgg cagaggcatc ttggcagcag cttttttacg gacgcggcgg
      241 agctggtcga ttgccatcgc ttcatccggc ccaacgcatt tgcgcagcgc cttttgtaat
      301 gacagcgggt cagccggcaa gtcggccgtc aaaaactcat ccgccgcacc gcttacaaga
      361 tattgcaaat cttttaatga taaacgcatc aaagtcttca aaaaaattct tacatcttaa
      421 aaaataggcc acagtcgtgt gcgtcgagaa taacggttgg ccgcattgga aaatcatttt
      481 tcctgcctgg cgttccggaa gatacgtttg acggcgtaag ttcgggcgtt ggtactttca
      541 aaatagattc gcatgagcat ctcgcctatg aggccggtcg agagaaagaa catgcccact
      601 atcagcagaa gaaatcccaa tgccatcagc ggcccatgct cttcgaaaag ccggaactcg
      661 ttccacgcaa aaatcttcct taaaaagcca taaaaaagta ttaaaaggcc aaaggcgaaa
      721 ctggccatgc cccagcggcc gaaaaagtgc ataggcctcg tgaggtaacc ggtgatgaat
      781 tttagcgtga ttatgtccaa cgcaacacgg aatgttctgc caatgccgta attgcttttg
      841 ccagctgccc tgcggacatt ttttatgggc acttcgcaga ttctggcgcc gagccgttgg
      901 cagaccgccg gaacaaaccg gtgcatgtcg ccgtaaagtc ggacatcttc aagaacttca
      961 cggcgatagg ctttgaatgt cgtgccgaaa tcatgcagcg ccacgccgct gacggcggcg
     1021 agcagccggt tggcaatttt ggacggcaga cggcgcatga cgaagttgtc gccgcgacgg
     1081 atgcgccagc cgctggctat gtcgtaacct tcgcgaattt tttcaatgaa catcggaatt
     1141 tcatacgggt cgtgctgcaa gtcgccatcc attgagatga ttatttcacc gccagccaca
     1201 tcgaaaccgg ccatcagccc cgtagtctgg ccaaagttgc gccgcaggtc tacaaaaacc
     1261 acacgcggat tggctgtggc gattttctcc aatatggctg gcgtatcgtc acgactgccg
     1321 tcgttgacat atacaagctc atattcgccg cccacgctat ccatgacctt tacaaggcgg
     1381 tcatgaagtt tttcgattat gtcctgctcg ttatagaacg ggacgacaat gctgtatgtt
     1441 atttttttag aggtttccat aatttatccg ttcaatcccg tttgcatcga acgcctttcg
     1501 cacgtcgtcg tcgcaaagtg cggcgagttc aatctgccga ctttcgataa gtctcgttac
     1561 cgattcgtcc aaatcatcgc agtaccctgg atgagtcatg atttcaatgg ttttacatgg
     1621 acagactttt acaagtgcgc cggcggcagt caacagccac tcgcgtgtaa tgcagcccgt
     1681 atgcgccacg ccccacgtgc cagccgttct gaaaaacctc cagtgacgca gtttgttcat
     1741 atcgcccaag aaagtcaata tattggatat gtgtttctgt ttggccggcg cttttggcca
     1801 tccgacccac ggcagatgtt cgccaagacg acgaatgaac gggatgttgt attttttcgc
     1861 caactctgcc actatcgaac agacggggcc aaatccgtgg ctgtgcctgt gcgtgtcaag
     1921 gtgcgtcggc cgaatgccca tggcaacggc acgtcgtatt tgagcgtcaa actcttccct
     1981 gacggctgaa aggagattgg gctttttaag cagaagcatg attataccta cggcagtgtg
     2041 gttcatcatg ccgctttcat ctgccagttt catgccggct ttggagatgc agcggccctg
     2101 cgatatgttc aggtgaacgc cgacgccgag cgtcggaact tccgccagca gtgaggctgc
     2161 atattcggat gcgtcgccgt tagcctgaaa cgtcgctgac gtcaaaatgc cgtacttgtg
     2221 ggcatgaata atcccttccg atacccgccg tgaaaggcca aaatcgtcgc cgttgattat
     2281 gactttacat ttttttcctg tttccatatg aaaccgaaat tatataataa ttcccatgcg
     2341 gtatccagta gaaggaactt tttaaagaac ctatctgtaa caattattgg gataggttct
     2401 atgtttatct taaaagaatg gacttatagc gatgttcggc aaaatatttt taaaattatc
     2461 ggcggctgac atttggatgg ctgtcggatt ttttgggcag gtggttttcg gccttcgatt
     2521 cattgtgcaa tggattgcca cggaaagagc gaaaaaatca ataataccgc tggcgttctg
     2581 gtatctgtcg ctggttggga cgctgatact gctggcgtat tcgatttaca gaaaagatcc
     2641 ggtttttatc gcggggttca gcctgaatct gataatctac gtgcgcaatc tatatttcat
     2701 acatattcat cccagaaaat gcgccgccgc caaaaaggaa atcgccgcaa atgacgagca
     2761 ataacgacct ctacagcgac cgcaggaaca ttttattgct gattcttctg tgcgtacttt
     2821 tgtatgttcc cggcatatcg tcttttcctt tgtgcgagcc ggaagaagcc cgctgcgcat
     2881 tgaccaccag cgaaatgctc caatcgggta attggctgat gccgacgctc tgtgggaaga
     2941 attattataa caaacctgcg ccgtattatt ggctgacggc tggcgttcag tggctgacgg
     3001 acgacagtga atttgccggc cggttagtgt cggccatttt tgcggcgttt gcggttgtgc
     3061 tggcatacaa gctggtacgg aaattcgaga gaaaatcggt agccatgctt gccggcatta
     3121 tgctcgcgac gtgcatggaa ttctggataa tgggccgtta ctaccgcatg gatatgccgt
     3181 tcgtgacgtc gatgtgggcg gcgatgtatt atttcgcatc gcgggagacg tgtgccgttg
     3241 tcgacaggcg cgcaggcaat tcatgctggc gcggattcta tttctgggct gccgtggcga
     3301 cgcttttcaa gggcccggcc ggtttggcgc tgccggtaat ggtcgtgggc acgtatcttc
     3361 tggcaacgcg ccagtttcgc cgcatacttg aattcttcaa tcccgccggc atggcgattt
     3421 acctgctggt ggcgtgtccg tggttcattg cgacatcgat aaaatatccg gaatattttg
     3481 gggcgttttt cggcagggaa aatttcggcc gggtcaccgg cggcggagat ctcggtcatt
     3541 catggccggg aattttatac attcccatac ttttcgccgg ctgcctgccg tggtgcgtgt
     3601 acattcctgg cgcatttatc agatacgtgc cgatgttttt cggaaaaacc ggacgggaaa
     3661 aaattatcgt attcctgatg acggcgacgg tcatgccact gctgtttttc atgttcagcc
     3721 acacaaaact ggtcagctat atcctgccgg tatttccgcc gctcatggtt atgctgggcg
     3781 tatttgtcgg cagatggtca gcgtcaagcc agccggacaa actcatgcga atcggcgcat
     3841 ggacactgat ggaattcgtt gcagtaatga ctgtcgtaat cgtcggtttt gaatggaaat
     3901 tcagatggct ggacttatct gccgttgtgc cgtgcgtgat tgttttctat gcgatattcc
     3961 gcatggcacg gtttatccgc acggaacgac gagtcagatt catacactgg tcggcgggat
     4021 gcgtggtaat gttgcagcta tatatgctgg tgcatatcgt tccttctggt ctggagtttc
     4081 tttcgtcccg cagagaagca caactgctgc cgcaggcagc aacaaacgtc tgtctttatc
     4141 ctgacgaaaa tctttcgttc atgttttaca ccggccatta tactgcgccg gtaatatcca
     4201 aacgcgatac tgccgcattt gaaaatctaa ctgccgaact gaattccagc cggccgatat
     4261 ggtgtttcgt cagcggacag aaatatttga aaaaactgca acaggctgtg ccggcggtca
     4321 aaatcatcga ctccaggaat gataatttcc tggtcactaa ctcatctgga acaacattgt
     4381 cgcaaaacgc tgtccagccg cttaaaaacg tcgaaaaata aggatttttt cacataaaag
     4441 aaggcgaaaa tcactcgctt ttttctgccg tttctgtcat aatatcgtga ttcgtaaagg
     4501 attgattttt tcgtcggcta accgcaaatc ggcagcccgg cggatttgtt tttacgatag
     4561 aaaggaatgg ccatggaaca ggccgaagaa gtcacgaacg ctcagaaaaa agccaccgtc
     4621 gaatacggtg aaaatcagat aaaagtactc aaaggccttg aggctgtgcg taaacggcca
     4681 ggcatgtata tcggcgatac cacgtcgcgc ggcttgcatc atctggtgtg ggaaatcgtc
     4741 gacaactcca tcgatgaagc catggccggc cgctgtagca gcatcctcgt gacaataaat
     4801 ctcgacgggt cggtgtcggt cacagacgac ggcatcggca ttccggtggg cattcatccg
     4861 acagagcaga tatcgacact tgaagtggtt ttctgcaagc tgcatgccgg cggaaaattt
     4921 gaagaaggcg catacaaggt ttcaggcggc ttgcacggcg taggcgcgtc ggttgtcaac
     4981 gccctcagcg aatggctcga agccgatgtc tgccgcgacg gcgatgtgta tcagatgacc
     5041 ttctctcgcg gcgtaaaaac gcaggaattg accaaaatcg gcaaacgcaa aaaaaccggc
     5101 actaaagtca cgttcaagcc ggactctcaa attttccccg atacggaatt ccgttatgaa
     5161 atactggcca gccggctgaa agaactggca tatctcaacg aaggcctgaa cataaaactt
     5221 cacgacgaac ggaccggccg cgaagacagt ttctgctatt cgcaaggcct gctggcgttc
     5281 gtcaaaaatc ttaacgaaga taaaatcacc tttcataagc ctatccgcat aacccgcctc
     5341 gacgaagccg ccaaaatgtc ctgcgacatc gtcatacaat ataacgacag ttatacggaa
     5401 aacgtctttt cgtttgccaa caatatcaat acgcatgagg gcggcacgca catgtcgggt
     5461 ttccgttctg cgctcacccg taccatgaat gcttatgcga aaaacaacag ccttatgaaa
     5521 aatgtcaaga ccgtaccgac aggcgacgat cttcgcgaag gcctgacggc ggttgtttcc
     5581 gtaaaactca cgaatccgca gttcgaaggc cagacgaaga ccaagctcgg caacagcgag
     5641 atggaaacat tcataaccac gacggtcaat gagcttttgg gtgcatggct ggaagagaat
     5701 ccgactgaag ccaaaaagat tatcaacaaa gccatctccg ccctgcaggc tcgcgaggcg
     5761 gcacgtaaag cccgcgattt gacccgtaga aaaggcgcgc tttccagcgg ctcactgccg
     5821 ggcaaactgg cagattgccg cagccgcgac cgcgattcga ccgaagtatt catcgtcgaa
     5881 ggtgattccg ccggcggttc cgccaagcag ggccgtaatt ccaataccca ggcgattctg
     5941 ccgctgcgtg gaaaaattct caatatagaa aaagcgcggc tcgacaagat tctcagttca
     6001 aacgaaattc aggtcatcat ttccgcgctg ggctgcggca taggcgaaga cgaattcgac
     6061 ttggacaaac gtcgttataa caaggttata ataatgactg atgctgacgt cgacggcagc
     6121 catattcgta cgctgctttt gacgtttttc ttccgccaca tgaaaccgct tctcacgcaa
     6181 ggccacatat tcatcgccca gccgccgctt taccttgtta cacggaagag acacaaagaa
     6241 tacgtcatca atgaaaaaca gatgcaaacc acgcttctga atctgggtct ggaaggcacg
     6301 acgcttgaag tccacgatac ggcccaaacg ccggctgttg ttgtaagctc attcaaaggt
     6361 gcaaaacttc gggaagtggt ggagttgctt gacagtctga atgacaatat tcatattctc
     6421 gaacgtcgcg gcattacgtt cccgatgctt ttgcagcatc gcgggcctga cggcaaactg
     6481 ccgacgcact ggatggtcat ggacagccgc aatcttttct ttgcctccgc tgaaaaatat
     6541 gccgaatttt tacaaaagca cgccgataag ctcgccgcta ctgaagataa tgaaaacggc
     6601 catgtcgaaa gcgatgaaaa catgcctgtt cacgaagtcc gcaaacgggc tgaattgcat
     6661 gaagtccgtg aaatggaaaa aacaatcgaa aaactcgccg ccaaagagtt gtttatcgac
     6721 gactacctga ttaaacgcga agaatccgtc actggcgacc ttgctccggc aaagtacgta
     6781 ctggttacag aagaggaatc gcttgaactt aacaacattt ccgaaatagc gtccggcgta
     6841 cgtacgttgg gtggcagagg cattgaaaca aaacgattta agggactggg cgaaatgaac
     6901 gccgaacagc tttgggaaac caccatggat cctgagcgtc gcatacttcg ccgcgtcaaa
     6961 gccgaagagg ctgaagatgc tgaacgcatg ttctcattac tcatgggcga caacgtcgaa
     7021 cgacgacgtc aattcatcga agataacgct cttaccgtaa aaaatctgga tgtgtagagc
     7081 gattctctgt tttttataac aaaacaaaaa ggcctgccga catttttgat aaatatcgac
     7141 aggcctttta ttttgagggg atgtttgatt acatcatgtc atccatgccg ccgccgggca
     7201 tggcgggttt gtctttcttt tcaggcaaat cggagatgac agcgtcagtg gtcagcagaa
     7261 ggccggcaac gctggcggcg ttctgcaggg ctgagcgttc aaccttggtc ggcacaatta
     7321 cgccgtcttt cacgaggtcg caatatttgc cggttaacgc gttatagccg aaattggcga
     7381 ttttttcggg attttccata actttcatcg ctacgacgct gccgtccaga ccgctgttgg
     7441 cggctatctg cttgattggc gccgacaatg cgcggcgaat tatatccacg ccggtctttt
     7501 cgtcttcgtt tgacgtcttg accttgtcga ggactttgac ggcagccagc acagctgtgc
     7561 cgccgccagg cagaatgcct tcttcaactg cggcccggca ggcgtgcagg gcgtcttcga
     7621 cgcgggcttt cttttccttc atctcgactt cggtggctga gccgacgttg atgcgggcga
     7681 cgccgccggc gagcttggcc agacgctctt cgagtttttc acggtcataa tcgctggtgg
     7741 tggtttcaat ctgggtcttt atctgttcga tacggcctct gatgtcgctt tccttgcctg
     7801 cgccttcgat tatggtcgtg gtatcttttg cgacgcggat ttccttggct gtgccgaggt
     7861 cggtcatctg gatgttttca aggctgatgc ccatgtcttc catgatggct ttgccgccgg
     7921 tgagcgttgc gatgtcacgc agcatttcct tgcggcggtc gccgaagccc ggggccttga
     7981 ccgccacgca ctggaacgtg ccgcgcagac ggttcactac cagcgtcgcc agcgcctcgc
     8041 cttccacgtc ttccgcgatg atgacgagcg gccggccggc ttgtgcgatt ttttccaaaa
     8101 gcggcacaag gtccttgatg gctgtgagtt ttttctcata tatcaggata agtggttttt
     8161 caagtttgca ttccatggcg gccatgtccg tcacgaaatg ggggctgatg tagcccttgt
     8221 cgaactgcat gccttcgacg aggtcaacat aggtgtcgag cgatttgcct tcctcgaccg
     8281 tgattacgcc gtccttgccg accttgtcca ttgcatctgc aatcattttg ccgatttcgg
     8341 cgtcctggtt ggccgagcag gcgccgacct gtgcgacctc ttttgaactc ttgacgggat
     8401 tggagagtgt gccgagttcg tcgatgattg cggcgacggc tttatcgatg ccgcgcttga
     8461 gttccattga atttgcaccg gcggttacat ttttaaggcc ttcttcaaag atggcttcgg
     8521 cgtaaatggt cgcggtcgtc gtgccgtcgc ctgcgatatc cgacgtcttt gacgcgactt
     8581 ccttgaccat ctgggcgccc atgttttcat tcgg
//
LOCUS       DAONFI010000062         8552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9549_length_8552_cov_4.197364, whole genome shotgun sequence.
ACCESSION   DAONFI010000062 DAONFI010000000
VERSION     DAONFI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9549_length_8552_cov_4.197364"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLK08_03120"
     CDS             <1..783
                     /locus_tag="PLK08_03120"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPS52522.1"
                     /translation="GGSVSRKFAPAAKLAGAIMVDNTSAFRMEKDVPLVVPEVNPEKI
                     KEHKGIIANPNCSTIIMNVPVWPLHKANPIKRLVVSTYQAASGAGAAAMAELKDGCRA
                     FLAGEPFQPKVLPHPAAFNVFSHNSKVGPNGYNEEEMKMLNETRKIFGCPEIRIAATC
                     VRVPVLRAHSESINIEFTNPMTEEQVREILADAPGVKIVDDREHSYFPMPRDASGKDD
                     VLVGRIRQDMSQPEGMGIEMFVSGDQIRKGAALNAVQIAELL"
     gene            894..1460
                     /locus_tag="PLK08_03125"
     CDS             894..1460
                     /locus_tag="PLK08_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPS52523.1"
                     /translation="MSMNKEEITNDGLGNRDGIWAPWRMVYLEALAGEKCDCFFCKYR
                     DEPEKDSQNLVVWRGRKAFLVLNRFPYTGGHCMVVPYEHIGRLDGLAEDTLVEIMLML
                     RDAQSVIRHAVKPHGFNVGMNLGRCAGAGLPDHLHAHIVPRWEGDTNFMPVLGQTHVI
                     PEFLSKTRQRFVDSAVELGLNSRYTMKD"
     gene            1465..2580
                     /gene="dgt"
                     /locus_tag="PLK08_03130"
     CDS             1465..2580
                     /gene="dgt"
                     /locus_tag="PLK08_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HPS52524.1"
                     /translation="MIALAAYAVPPTADSRNYPEKPDEFRSGFQRDRDRILHCSAFRR
                     LDFKTQVFVPHEYDHFRTRLTHSLEVSQIGRDVGRALGLNEDLIEAVALAHDLGHPPF
                     GHIGEKVLDELMASYGHFEHNRQSLRVVDYLEHPYPSYRGLNLTQMTRICLAKHETRY
                     DSPADNEFTKIRFAPLEGQLVDLADEIAYTTADLEDALEADWLSMVDLNELELWRQAW
                     ATVEAEYPDARKIHKRIRSTKKILNMMAVDAITYAGEKLSGMKPASYEKVMDGPQKII
                     TFSPDMEKLIAPLHALLMGKVYCHSSNMLNDEFARVAIMDLFCYFTTNPGKLPQRYLV
                     RVEDGCDSLQRVICDYLAGMTDRFCRNLYDSIKLEKK"
     gene            2767..4830
                     /gene="uvrB"
                     /locus_tag="PLK08_03135"
     CDS             2767..4830
                     /gene="uvrB"
                     /locus_tag="PLK08_03135"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPS52525.1"
                     /translation="MSDFKIVSSLKPMGDQPAAIDSLVNNIARQQKFNVLMGVTGSGK
                     TFTMANVIERTQRPALVISHNKTLAAQLYEEFKSLFPDNAVEYFVSYYDYYQPEAYIP
                     QRDIYIEKDAARNDDLDRLRLSATTSLSHRRDVIIVASVSCIFGLGSPEQYKKAVVVA
                     DVGTDVDRNEFLAQLVNLQYDRNDVDFKRGTFRVRGDTVDIYPAYEEFGYQVEFFGDT
                     IDALRFINPTTGETLSVTDKLFIFPAVHYVVAEDAVENAVTGIRRELESRVIELKNAG
                     KLLEAQRLSARTMYDMEMMLEVGYCSGIENYSRWLDGRKAGEKPYTLIDYFPEDFLLF
                     IDESHVTLPQLRAMYNGDRARKQVLVDHGFRLPSAMDNRPLRFDEFENMWNQVVFVSA
                     TPGEYELECSGGEVVEQIIRPTGLVDPAIDVRPARGQVADILTEVGKRVIAGQRTLIT
                     TLTKRLAEDLSDYIRKEGYTCQYLHSEVDTLERVEILKQLREGKYDVLVGVNLLREGL
                     DLPEVSLVCILDADKQGFLRSATSLIQTIGRCARNVNSQVLLYGDTVTPAMQKAIDET
                     SRRREIQLAYNKEHGITPKTVEKKIRDTLTEQIKARQIARDAIGINEEQFERTELLAE
                     LEREMLAAAESLEFEKAARLRDRIAELKTADSIEDDDDSLAEKTSAYKPPKKTTRGKK
                     HKQNR"
     gene            complement(4840..5334)
                     /locus_tag="PLK08_03140"
     CDS             complement(4840..5334)
                     /locus_tag="PLK08_03140"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPS52526.1"
                     /translation="MKIYTRKGDDGTTCDLSGARVKKNSPAIVACGEIDELSAAIGLV
                     RPLAATPNSHQLNDTLTELQRKLIQMAAVVSDTAWIKNIDFPKEIELLEKLIDKYAAM
                     NGDMKTFIIPGDNELSARMNFARTICRRTERSLVTLAETREKLQPVLAWFNRLGDLLF
                     EMSR"
     gene            complement(5315..6205)
                     /gene="pssA"
                     /locus_tag="PLK08_03145"
     CDS             complement(5315..6205)
                     /gene="pssA"
                     /locus_tag="PLK08_03145"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPS52527.1"
                     /translation="MINEHRRHRRTIKTRMLKSTSVLPAMFTLCNGVAGFIAIHYATK
                     QGVLGGSACLGEEAARNLCIAAWLMAVAMAFDMLDGRVARMTRTTSDFGAQLDSLCDM
                     ISFGAAPAIIMVRASVGILREEYYFLSLERLILCIAAVYVACAAIRLARFNVETDADE
                     SAHMSFSGLPSPGAAACVGATVLCFMRVIELHISWLPPKAVIVSMSFFLPVVTLCAAL
                     LMVSRIRYPHVVNQYLRGKKPLNYLIIIILLLVLFISLSIQVAMGIICVIFVLTPPLK
                     ALRHRLMQRQKKPANENIYA"
     gene            complement(6202..6876)
                     /locus_tag="PLK08_03150"
     CDS             complement(6202..6876)
                     /locus_tag="PLK08_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPS52528.1"
                     /translation="MITRHGIREVFMCGSLALAATAGIYFLASRCGETWWLIEILPII
                     FFGWVLWFFRDPNRRTPTEPGLFISPADGRIADITPIGPDGRLGRPGVEIGVFMNVFS
                     VHVNRMPCSAVIENIVHVPGTFMDVRDPQSYEKNESATLTLSYNHNGMTYPVIVRQIA
                     GLVARRIVTAVRPGQRLERGERFGMIKFGSRLELYLPQELAAEICVAVGQKVSAGETV
                     LAKVKE"
     gene            7155..>8552
                     /locus_tag="PLK08_03155"
     CDS             7155..>8552
                     /locus_tag="PLK08_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52529.1"
                     /translation="MRFRSSAVLAVTARFTCRYCGTTFTSKIMEWQDDVPMPTAQEVY
                     PYLSADLSDFEEKPAETTQQIPPSAPVIDFRREQPPHSQIQSSREPQIAAWAAGTDID
                     EDAEITAQPKIAPRQEVCCNTTTSKIQPAETAELRSQPHGDEDSAADMSADNLNETTD
                     TCTSPVFIQDMQEDTAEDEVVENIVEFPKFLKRPSQLRRNDAREMVIETASSAELLER
                     PEIVIDGDASEISTLIIEEEELRCGVAVNDGRISKFNRDILFYLMLSAIAFAIVGGLL
                     WMIIAGGKSVDSKNVIPDFNAAIRNDIEKRFEQNSPKSVLTAAAAAENSIEEIVTILP
                     QPVVAAEPSSGSSAAVGAAVVVSPVAVPAAAKSVAAEKMSVGKTSNLEIESARVEMAI
                     INADGKLLRLTSRSCPTMACGGEIVFDVSAAGDSDSSDCEVERETISTVDPIAMKILS
                     ISRRGGLLGAAAEAAV"
BASE COUNT         2081 a         2123 c         2360 g         1988 t
ORIGIN      
        1 ggcggcagcg tatccaggaa attcgcgccg gcagccaaac ttgcaggcgc gataatggtg
       61 gacaacacct ccgcattccg gatggagaaa gatgtaccgc tcgtagtgcc ggaagtcaat
      121 cctgagaaaa tcaaggagca taaggggatt attgctaatc ccaactgctc gactattata
      181 atgaatgtgc cggtatggcc gctgcataag gccaacccga taaaacggct ggtcgtcagt
      241 acgtatcaag ccgccagcgg cgccggcgcc gctgcgatgg cggaactcaa ggacggctgt
      301 cgtgcgtttc tcgccggcga gccgttccag cccaaagttc tgccccatcc agccgcgttt
      361 aacgtcttca gccataattc caaagttggc ccaaacggct ataatgagga agaaatgaag
      421 atgctcaacg agacgcgcaa gattttcggc tgtccggaga ttcgcattgc ggctacgtgc
      481 gtgcgagttc cggtcctgcg tgcgcatagc gagtccataa atattgaatt taccaatccg
      541 atgaccgaag agcaggtgcg ggaaattctc gccgacgctc ctggcgtgaa gattgtggat
      601 gaccgcgagc acagttattt tccgatgccc agggatgcgt ccgggaagga tgatgttctt
      661 gtcggaagaa ttcggcagga catgtcccag cctgagggta tgggcataga gatgttcgtc
      721 agcggcgacc agattcgcaa aggcgccgca ttgaatgcag tccagattgc cgaactgctg
      781 taaacatgga tgaaaataac tgtcgtcagt atgaatatgg tagtcatttt aaaaacccgc
      841 agatttttgc gggttttttt atttcgtcaa aatgtgtaaa gttgctatca taaatgtcta
      901 tgaataagga agagataacc aacgacggtt tgggtaaccg ggacgggata tgggcgccgt
      961 ggcggatggt ttaccttgaa gcgctggctg gggaaaaatg cgactgcttt ttctgcaaat
     1021 atcgcgatga gccggaaaaa gattcccaaa atcttgtggt atggcgaggc aggaaggcgt
     1081 ttctggtcct aaaccgtttt ccgtatacgg gcgggcattg catggtcgtg ccgtatgaac
     1141 acattggccg cctggacggg ttagccgagg atactctcgt cgagataatg ctcatgttgc
     1201 gtgatgccca gagcgtcatt cgtcacgcgg taaagccgca cggttttaac gtcggcatga
     1261 atctgggccg ttgtgccggc gccggcttgc cagaccacct gcacgcgcat atagtgccga
     1321 gatgggaagg tgataccaac tttatgcccg ttctcggcca gacgcacgtc atccccgaat
     1381 ttctcagcaa aacacgtcag agattcgtcg attcagccgt tgaactgggc ctgaattcaa
     1441 ggtacaccat gaaggactga ctgcatgata gcattagccg cctatgccgt gccgccgact
     1501 gccgacagca gaaattatcc tgaaaagccc gacgaattcc gtagcggttt tcagcgggac
     1561 agggacagga ttctgcattg cagtgcgttc aggcggcttg acttcaagac gcaggttttt
     1621 gtgccgcatg agtatgatca ttttcggact cgtctgacgc attcgcttga agtttcgcag
     1681 ataggccgtg atgtcggcag ggcactgggc ctcaacgaag acctcataga agcggtcgct
     1741 cttgcccacg acctcggcca tccgccgttc ggccatatcg gggaaaaagt acttgacgaa
     1801 ttgatggcga gctacggcca ctttgaacat aatcggcagt cgttgcgggt ggtggattat
     1861 ctggaacatc catatccgtc ctataggggt ctgaatctca cgcagatgac gcggatttgt
     1921 ctggctaagc atgagacgcg ttatgattcg ccggctgaca atgaatttac gaaaatccgt
     1981 tttgctccgc tggaaggcca gttggtggac cttgccgacg aaattgccta cactaccgcc
     2041 gacctggaag atgccctgga ggctgactgg ctgagcatgg tcgatttgaa cgagctcgaa
     2101 ctctggcggc aggcatgggc gacggtggaa gcggaatatc cggacgctcg caaaattcac
     2161 aaacgaatcc ggtcgacaaa gaaaatcctg aacatgatgg ctgtagatgc catcacttat
     2221 gccggcgaga aattgagcgg aatgaaaccc gcttcatatg agaaagtcat ggacggcccg
     2281 caaaaaataa tcaccttcag tccggatatg gagaaactca tcgccccgct ccatgcgctg
     2341 ctgatgggta aagtctactg ccacagctca aatatgctga atgacgagtt tgcccgcgtg
     2401 gcaattatgg atttattctg ctattttacg acgaatccag gcaaactgcc gcaacgttat
     2461 cttgttcgtg tggaagacgg ctgcgattcg cttcaacgcg tcatctgtga ttatcttgcc
     2521 ggcatgacgg accgtttctg tcgcaatctt tacgactcaa taaaattgga gaagaaatga
     2581 cggcgacgtt tcaatgccct atcggttttc cggcagaaag aattttcaat aatcccgctg
     2641 aaaagtgaaa aagcaatttt tgcattttag agcataaaat tctgcgagtt aaaactgtag
     2701 aactacgaag aatacgtaaa aatttctacg agttaaaacc gtagaattat gaagaacata
     2761 taaaaaatgt ctgattttaa aattgtatca tccctaaaac ccatgggcga tcagccggct
     2821 gcgatagact cgttggtgaa caatattgcg cggcaacaga aatttaacgt gctcatgggc
     2881 gtgaccggca gcggcaaaac gtttacgatg gctaacgtta tcgagcgcac ccaacggccg
     2941 gcactggtca tcagccacaa taaaacgctc gccgcccagc tttatgagga attcaagtcg
     3001 cttttccccg acaatgccgt cgaatatttc gtgagctatt atgattatta tcagcccgaa
     3061 gcctatatcc cccagcgtga catttatatc gagaaagacg ctgcgcgtaa tgacgacctt
     3121 gacagattgc ggctgtcggc cactaccagc ctttcgcatc ggcgggacgt tataatcgtt
     3181 gccagcgtat catgcatttt cggcttgggc agtccggaac agtacaaaaa agccgtcgtc
     3241 gtcgccgacg tcggtacgga tgtcgaccgc aatgaatttc tggcacagtt agtcaatctg
     3301 caatatgacc gtaacgacgt ggatttcaaa cgcggcacgt tccgtgtccg tggcgatacg
     3361 gttgacattt atcctgcata cgaggaattc ggataccagg tggaattttt cggcgatacc
     3421 atagacgccc tgcggttcat aaatccgact accggcgaga cgctcagcgt gacggacaag
     3481 ctttttattt ttccagccgt tcattacgtg gtggctgagg atgcggtcga aaatgccgtt
     3541 acgggtatcc gccgcgaact ggaatcacgc gtgatagagt tgaaaaatgc cggcaaactg
     3601 cttgaggctc agcggctttc agcccgcacc atgtatgata tggagatgat gcttgaggtc
     3661 ggttattgca gcggcataga gaattactcc cgttggctgg acggccgaaa agccggcgaa
     3721 aagccgtata ctctgataga ttattttcct gaagattttc tgcttttcat tgatgaatcg
     3781 cacgtgaccc tgccgcagtt gcgcgcaatg tacaacggcg accgcgcccg caagcaggtg
     3841 ctcgtcgacc acgggtttcg tctgcccagt gcgatggaca accggccgct gcggtttgat
     3901 gaattcgaaa acatgtggaa tcaggtcgtt ttcgtctcag ccactcctgg cgaatatgaa
     3961 ctcgaatgca gcggcggcga agttgtggaa cagattatcc ggccgactgg actggttgac
     4021 cctgcaatag acgtgcgtcc ggcacgcggg caggttgcgg acatattgac cgaggtcggc
     4081 aaacgcgtca tagccggcca acgaacgctc ataacgacgc tgaccaagcg gctggctgag
     4141 gatttgagcg actacatccg caaggaaggc tatacgtgcc aatatcttca cagcgaggtc
     4201 gatacgcttg aacgagtgga aattctgaaa cagttgcgtg aaggcaagta cgatgtactg
     4261 gttggcgtga acctgctgcg tgaagggctt gacctgccgg aggtttcgct ggtgtgcata
     4321 cttgatgccg acaaacaggg ttttctgcgg tcggcgactt cgcttataca gaccatcggc
     4381 cgatgtgccc gaaacgtcaa ttcgcaggtt ctgctgtacg gcgacacagt tacgccggcg
     4441 atgcagaaag caatcgatga aaccagccgc cgtcgcgaaa tacagcttgc ctataataaa
     4501 gaacacggca taacgcccaa aacggtcgaa aagaagattc gcgacacgct tacggaacag
     4561 ataaaagccc gccagattgc ccgcgatgcg attggcataa atgaagagca gttcgaacgc
     4621 acggagttgc tcgcggaact ggaacgggag atgctcgcgg ctgcagagtc gcttgagttt
     4681 gaaaaagctg cccgactgcg tgacagaatc gccgaactca aaacagccga cagtattgaa
     4741 gacgatgacg attctttagc tgaaaaaact tccgcctata aaccgccgaa gaaaactacc
     4801 cgcggcaaaa aacataaaca gaaccgatag agaattgctc tatctggaca tttcgaaaag
     4861 caggtcgccc agccggttga accacgcgag taccggctga agtttttccc gcgtttccgc
     4921 gagtgttacg agcgaacgct cggttcgccg acaaatggtt ctggcgaaat tcatacgggc
     4981 ggagagttcg ttatcgccgg gaatgataaa cgttttcatg tcgccgttca tggcggcgta
     5041 tttgtcgata agtttttcaa gcaactcgat ttctttaggg aaatctatgt tttttatcca
     5101 tgccgtatca gacactactg cggccatctg tatgagcttt cgttgcaatt ctgttagcgt
     5161 atcgttgagt tgatgcgagt taggcgttgc agccagcggc cttacgagcc cgattgcagc
     5221 cgagagttcg tctatttcac cgcatgcgac tattgcgggc gaattttttt ttacccttgc
     5281 gccgctgagg tcgcatgttg tgccgtcgtc gcctttacgc gtatatattt tcatttgcag
     5341 gttttttttg tctttgcata agtctgtgcc gcagggcttt gagcggcggc gtaagaacga
     5401 aaattacgca tatgattccc atggctacct gtatcgaaag cgaaatgaac agtaccagca
     5461 gcaggattat tattatgagg taattgagcg gctttttgcc gcgcagatac tggttgacca
     5521 cgtgggggta tcgtatgcgc gataccatca gcaatgctgc gcagagcgtc acgaccggca
     5581 ggaagaaact catgctcact atgaccgctt ttggcggcag ccacgagatg tgaagttcga
     5641 ttacacgcat gaagcacagc acggtggcgc caacgcaggc tgccgcgcct ggcgacggca
     5701 ggccgctgaa gctcatgtgt gcggattcgt cggcatccgt ctctacgttg aatctcgcca
     5761 gccgtatggc tgcgcacgcg acatagacgg ctgctatgca cagtatcaat ctttcaagcg
     5821 aaaggaagta atattcttcg cggagaatgc cgacgctggc ccgcaccatt atgatggctg
     5881 gcgctgcgcc gaaacttatc atgtcgcaaa ggctgtctag ttgggcgccg aaatctgacg
     5941 ttgtgcgggt cattctggcg actctgccgt cgagcatgtc gaacgccatg gccaccgcca
     6001 tcagccatgc cgcgatgcag aggtttcggg ctgcctcttc gccgaggcac gcactgccgc
     6061 cgagtacgcc ctgcttggtg gcatagtgga tggcgataaa accggcaacg ccgttgcaga
     6121 gagtgaacat cgcgggcagt accgacgtgc tcttgagcat gcgggtttta atcgttctgc
     6181 gatgtcttct gtgttcgtta atcattcttt caccttggcg agcacggtct cgccggctga
     6241 tactttctgg ccgacggcga cgcatatttc ggcggcaagc tcctgcggca ggtaaagctc
     6301 cagccgcgag ccgaatttta tcatgccgaa ccgctcgccg cgttcaagcc gctggcccgg
     6361 ccggacggct gtgactatgc gcctcgccac gaggccggct atctggcgga cgattaccgg
     6421 atacgtcatg ccgttgtgat tgtaactcag cgtcagcgtt gcggactcgt ttttttcgta
     6481 gctttggggg tcacgcacat ccatgaatgt gcccggtacg tgtacgatgt tttcgataac
     6541 cgccgagcac ggcatacggt ttacgtggac gctgaagacg ttcatgaata cgccgatttc
     6601 cacgcccggc cggccgagtc ggccatcggg gccgatgggt gttatgtcgg cgattctgcc
     6661 gtcggctgga gatatgaaca ggccgggctc tgtcggcgta cgacggttcg ggtcgcggaa
     6721 aaaccacaaa acccagccga agaatattat cggcagaatt tcaatcagcc accacgtctc
     6781 gccgcaacgg ctggccagaa aatatatgcc cgccgtcgcc gccaacgcca gcgacccgca
     6841 catgaacact tcccttattc cgtgtctggt tatcatcgca tcaagtatcc acaattcgcc
     6901 tgtcgcgtca attattttat atggctgagc ctttttgacg gaaaagtcgg ctgagtcggg
     6961 ggatttgctt gcaggaacac tgccgtataa ctacaatatt cagcgatatt ttaaaataac
     7021 agataatgtt taaatgaata ttggcgtaaa actgcccgac ttttgatgaa aacgaaagac
     7081 gtggctggaa tgttggctaa aaaacgctgg aatcggatat gcatcttgta gtatgcccac
     7141 attgttggag gcaattgagg tttcgcagtt cagccgtcct ggctgtcaca gccagattta
     7201 catgcagata ctgcggtact acttttacct cgaagataat ggaatggcag gacgatgtcc
     7261 ccatgccgac cgcgcaggag gtatatccgt atctttcggc tgatttgtct gattttgagg
     7321 aaaagccggc agaaacaacc caacagatac cgccgtctgc cccggtaatc gatttccgac
     7381 gcgaacaacc gccgcattcg caaatccagt cgtcccgtga gccgcagatt gccgcatggg
     7441 ccgccggcac tgatattgac gaagacgccg aaataaccgc tcagccgaaa atcgcgccgc
     7501 ggcaggaagt ttgctgcaat acgacaactt cgaaaatcca gccggctgaa acggcggagt
     7561 tgcgttcgca gccgcatggc gatgaagatt cggcggcgga tatgtcggca gataatctga
     7621 acgagacaac ggatacctgc acctcgccgg ttttcataca ggacatgcag gaagatacgg
     7681 cagaagatga agttgtcgaa aatatcgtcg aattcccgaa atttctaaaa agaccttcgc
     7741 aattacggcg aaacgatgct cgtgaaatgg ttatagaaac ggcatccagc gcagaacttc
     7801 ttgaacggcc ggagattgtg attgacggcg atgcctctga aatttcaacg ctgattattg
     7861 aggaagagga actgcgatgc ggcgtagctg taaatgacgg cagaatatcg aaattcaatc
     7921 gcgatatttt attttatctg atgttgtcgg cgattgcgtt tgcgattgtc ggcgggctgt
     7981 tgtggatgat tattgccggc ggcaaatcag tcgattcgaa gaacgtaatt cctgatttca
     8041 acgcagctat acggaacgac attgaaaaac gatttgagca gaattctccg aaatcggttt
     8101 tgacggctgc cgcggcggcg gagaattcaa tcgaggaaat agtaaccatc ctgccgcagc
     8161 ctgttgtggc ggcagagccg tcgtccggca gttccgctgc tgtgggtgca gctgttgtag
     8221 tatcgccagt ggccgtgcct gcagctgcga aaagtgtcgc ggccgaaaaa atgtctgtcg
     8281 gcaaaacatc aaatctggag atagaatctg cccgtgttga aatggccata atcaatgccg
     8341 acggcaaact tctgcggctg accagccgtt catgtccgac gatggcctgc ggcggcgaga
     8401 tagtttttga cgtttcggcg gctggcgact cggattcgtc ggattgtgag gttgagcgtg
     8461 aaacgatttc gacggttgac cccattgcca tgaagatatt gagcatatcg cgtcgtggcg
     8521 gattgctcgg cgcggcggcg gaggcggcgg tg
//
LOCUS       DAONFI010000063         8545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9558_length_8545_cov_4.096231, whole genome shotgun sequence.
ACCESSION   DAONFI010000063 DAONFI010000000
VERSION     DAONFI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_8545_cov_4.096231"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLK08_03160"
     CDS             <1..694
                     /locus_tag="PLK08_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52530.1"
                     /translation="LEMREHMQTVRKSGLRALWMGVEDMTATLVKKGQSVNKTIEAFR
                     MLKDAGISPMPMMMHYDAQPLLTRGKPDGLLNQVKILRKAGAIGLQVLMITPATGSKM
                     YEDTFKSGMVYESVGRRKVEPYMLDGNYVVASNHKHPWTKQFNIMAAYLYFYNPLRML
                     YAICRPKNSLYLVDCGMQVIGMWGLSLTIRRTLGWAFRLMFCKIRRRTAPPSENLPMR
                     SPDGGTASHALL"
     gene            complement(1022..2338)
                     /locus_tag="PLK08_03165"
     CDS             complement(1022..2338)
                     /locus_tag="PLK08_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPS52531.1"
                     /translation="MILSLISTALILGYGYFMWRRGLFEALVMAVCCVFAAGVAVTCY
                     QYIASLAGLDKSQPSFGEPLAMGLLFLVSLFVMRFLADRFIKSEMHLPLYVDKAIAAI
                     LGVFSGIIMIGIPLTMLQMIPLGGRSIMDYKPFDDNLENYAKFSPFAPDDFVLGIGKV
                     FSAASMGNGHAMPSAQEQKLNWFIRRNSAGLGGTTLASQENLASVQEIYEIKSIRIDP
                     VRLSRSAEYKNYALRCVMDINATDDDRWWRLPATQFALFCRDNKGRMQRHFPIGYIIR
                     RSSQTAPGWSFDMLPSPNDAVRQPLCLLAIERSEKEVDPDEKGLVVDWVYQIPDDEQI
                     AELVFRNCKPFNLSCSMIKQGLPLNFRDLKKARITLAPKNEKEFMPVISNSSAKPAND
                     KFEQKVQKLPSVLIKQTTKDKLIKINEHEKALDAAVENQMNQKSAK"
     gene            complement(2335..3990)
                     /locus_tag="PLK08_03170"
     CDS             complement(2335..3990)
                     /locus_tag="PLK08_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPS52532.1"
                     /translation="MAASIISAITEPVMYGSPVKMAIAVLFSGIWLWSTVWLDRDAGR
                     FLLPRAAWSGAYLAAGVFGLFIWIIIGSYAVGIILFLAIAGGTFAGYVVFRNGRVNEA
                     FAIFTPAWFSRLKGGTSHKLEARTRIPLYDNAGRPVILTDEKLSNPMFVMNYNLTQDF
                     LFSVAAGRAAEAAVDPAGEWARCVYLIDGIAIGQQPLHTEDVNRIAAFIKDIGGMSDK
                     SDKPQKGMIGFDMAGKKIDVEATISPSNQGDKIRLRILNEVVQTNLDELGLSRKSLAI
                     LQECLEGSGMIIVSGLPKSGLTSTLYSITKKIDPYLRNIIAVEHKPAAELTNVSQHVY
                     NDREEYRQTLVNVLRHDPDVIMADGCFDSASAELLGDFAKNKLVIIAIPATETAEAIL
                     RWKKLGGSVDMVKAVTSQILLRKICTNCREEYPTTPELLEKMRLPANVTKFYRTPKIP
                     ADQNERRQLQYNPCPACNECGYFGRTAAFEVMQVDNAIRQAAESGNIAQIRETARKST
                     KMLTLQEQIFVKVAQGITSLEEAQRVLKIHANQLQQLKGGKAK"
     gene            complement(4094..5503)
                     /gene="cysS"
                     /locus_tag="PLK08_03175"
     CDS             complement(4094..5503)
                     /gene="cysS"
                     /locus_tag="PLK08_03175"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPS52533.1"
                     /translation="MIKLYNTMTRSVDEFKPVTPGRVGLYTCGPTVYNFAHIGNLRTY
                     VFEDILKRALKQAGYDVTHVMNVTDVGHLVSDSDEGEDKMALGAAREGKTVWEIADFY
                     WQAFRKDIARLNIIEPTVWCKATDHIAEQIEQVKILEAKGYTYRTADGIYFDTSKLSD
                     YGKLGRLDVKGLQAGIRIEMGDKRNPTDFALWKFSPTDKHRLMEWPSPWGVGFPGWHI
                     ECSTMAIKYLGERLDIHCGGIDHVNIHHTNEIAQAESTLGHEWCNWWMHGEFLTIAKS
                     DDSGGGKMSKSTGEFLTLDILVKKGYDPLAYRYFLLNAHYRQQLAFSFEGLDAAANAY
                     KNLTRHVLDLKAAAAGKTGNVIAGYYDKFTQAVMDDLNMPRALAAMWGAAKDSAATPA
                     DVLATLYKMDEVLGLNIAKMQEQTQNLDVAKIESLIAERTAAKRQKNFNCADAIRDEL
                     AAMNIELLDTPQGTTWRKK"
     gene            complement(5580..7046)
                     /locus_tag="PLK08_03180"
     CDS             complement(5580..7046)
                     /locus_tag="PLK08_03180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HPS52534.1"
                     /translation="MAEKQHFLAIDLGAESGRGIIVTLNNAKVSMEEIHRFPNRRITA
                     AGTMYWDFLHLFDEILRAIAIAAEKKIDITSISVDTWGVDFGLVGKDGKLLGNCVCYR
                     DSRTENIHEYSNQTMHTSEIFKLTAYEPWAISSLFQLLAMKRDKSPLLKVAGSFLNMP
                     DLFTYMLTGKKISEMSIANTSSLMGKDGKWCDEIIDAFSLPKMFGKLVEPATVVGTLT
                     PEVQKLTGMGPVEVVATCGHDTSAALAAVPAEGNNWAFLSCGTWSILGKLVDEPVATP
                     KCLKEGFTNEYTIGGWYLAQNIIGLWLVQELRRKWDRPEESWDYNRITAEAGTAKSSA
                     IVNVADTSLMAPHDMEEAIMALIEKTGQPKPKTRGELVRCVLESLALQYNTAIDSIAT
                     LTGKRPDALYIVGGGIKNKMLCQFTANACGMEVQAGCDQCTALGNGLGQALAMGVLKK
                     PDDIRKVMRNSFEITKYKPAEQNDWAKKREIYKNLRKA"
     gene            complement(7080..7964)
                     /locus_tag="PLK08_03185"
     CDS             complement(7080..7964)
                     /locus_tag="PLK08_03185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010998014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPS52535.1"
                     /translation="MPIPHKILKKTKNADPSAPQRQNNRNTTARRMEKSLGSRPNEKA
                     GIPAPTGKVRLHKYLAELGVASRRTIEEMIVEGRIVVNEHVVTELPFFVNPDEDRIKV
                     DGVNVRRKTAPKTYFLLNKPKGVVCTTIDQLGRPCVVDLLPPEFAKQRIYCVGRLDAD
                     STGAILLTNDGELTQHLTHPSHEVPKTYIVTVRGTIDGESFEKIRHGVYLDGRRTQGA
                     DVKILQKGQENTIIEMTLREGRNREIRRTLLRLGHKVKKLQRTAIGPLTVKGLGPGHM
                     RMLSKKEVESLWKAGKPD"
     gene            complement(8049..>8261)
                     /locus_tag="PLK08_03190"
     CDS             complement(8049..>8261)
                     /locus_tag="PLK08_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52536.1"
                     /translation="AGQPFTLPTGVCSAMMFFGGDAEINHAGDAEPTTQVTAGNTILL
                     PATLEEPVLQAKTDADWLEITLPARQ"
     assembly_gap    8262..8271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8272..>8545)
                     /locus_tag="PLK08_03195"
     CDS             complement(<8272..>8545)
                     /locus_tag="PLK08_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPS52537.1"
                     /translation="VKRYDCRKGDFYHLPAGTVHAVGPGLVIAEVQTPSDTTYRVTDW
                     GRGREIHVERSMQCIHFAPTPDNASGAAGDTLWVTDYFTVSHRVTKA"
BASE COUNT         1750 a         2144 c         2271 g         2370 t
ORIGIN      
        1 tcttgaaatg cgcgagcata tgcagaccgt cagaaaatcc ggattgcgtg cgttatggat
       61 gggcgtggaa gacatgaccg ccacgctcgt gaagaaaggc caatccgtca acaagaccat
      121 tgaagccttc cgcatgctca aggatgcggg catatcgccc atgccgatga tgatgcacta
      181 tgacgcccaa ccgctactga ctcgtggcaa acccgacggc ctgcttaacc aggtcaaaat
      241 actccgcaaa gccggtgcca ttggtcttca ggtactgatg ataactccag cgaccggctc
      301 gaagatgtac gaagacacat ttaagtccgg aatggtttac gagagtgtcg gtcgacgcaa
      361 ggtcgaaccg tatatgctcg acggcaatta tgtcgtcgcg tcaaatcata agcatccgtg
      421 gaccaagcag ttcaatatca tggcggcgta tctttatttt tacaatcctc tgcgtatgct
      481 ttacgctatc tgtcggccga aaaattcgtt atatctggtt gactgtggca tgcaggttat
      541 cggcatgtgg gggttgtcgt tgacaatacg tcgaaccctt ggctgggcgt ttaggctgat
      601 gttctgtaaa atcagacgta gaaccgctcc gccgtcagaa aatctgccca tgcgcagccc
      661 ggacggcgga actgccagcc atgccttact atgataaatc cggatggatg tgaaattcca
      721 atccttatcg aaattatggc cacagagaat gttccacaat cgcataacca tgaatggttc
      781 aatttgtata ggttcaagtt aaagaacatt tgaaatatgt cctttttgta atatttttca
      841 atacgccgtc tttcttgagc ttttcaaatg aattttcgct ccccattcca agagtacctg
      901 acaggcggct atggaataaa tttgcttcag ctgtgatgat atggttttca gccagtggaa
      961 atttactgtg tgagaaatct tgaagttaga attcaatctg catgtttaca actctaccgg
     1021 cttactttgc agatttttga ttcatttgat tttcaacggc agcatcgagt gccttttcgt
     1081 gttcattgat ttttattaat ttgtcctttg ttgtctgttt tataagcacc gacggcaatt
     1141 tttggacttt ttgttcaaat ttatcgtttg cgggttttgc tgatgaattt gaaattaccg
     1201 gcataaattc cttttcattt tttggggcca gtgtaattcg ggcttttttc aaatctcgaa
     1261 aattcagcgg caatccctgc tttatcatgg aacagctcaa attaaacggc ttgcagttac
     1321 ggaataccaa ttcggcaatc tgttcatcat ccggaatctg ataaacccag tccaccacca
     1381 gacctttttc gtcggggtct acctcttttt cgctacgttc gattgccagc aggcatagcg
     1441 gttgtctgac agcatcattt ggcgacggca acatgtcgaa cgaccagcct ggcgccgttt
     1501 gagaagaacg tcgtataata tagcctatcg gaaagtggcg ttgcattctg cctttattgt
     1561 ctcggcagaa cagggcgaat tgcgtagccg gcagccgcca ccatctgtcg tcgtcagttg
     1621 cgtttatgtc catgacgcaa cgcaatgcgt aatttttgta ttccgctgaa cggcttagac
     1681 gtacagggtc tatccttatg cttttaattt cgtagatttc ctggactgat gccagatttt
     1741 cctggctggc caacgttgtg ccgccgagac ctgcgctgtt gcggcgaatg aaccagttga
     1801 gtttctgttc ctgtgctgag ggcatggcgt ggccgttgcc catggaagcg gctgaaaaaa
     1861 ccttgccgat tcccaacacg aaatcgtctg gcgcaaaagg agaaaatttg gcgtaatttt
     1921 ccaaattgtc gtcaaacggt ttatagtcca ttatgcttct gccgccgagc ggtatcatct
     1981 ggagcattgt cagcgggatt cctatcataa ttatgccgga gaacacgccc aatattgcgg
     2041 cgatggcttt gtcgacatag agcggcagat gcatttcaga tttgatgaac ctgtctgcca
     2101 ggaatcgcat tacgaacaga gataccagga acagaaggcc cattgcgagc ggttcgccga
     2161 aagacggttg tgatttgtcg aggccggcta aagatgcgat gtattgatag catgttactg
     2221 caactccggc ggcgaatacg caacagacgg ccattaccag ggcttcaaac agccctctac
     2281 gccacataaa atagccgtaa cccaatatca gggctgtgga gattagcgac agtatcattt
     2341 tgccttacct cccttgagtt gctggagctg attggcatgg attttcagaa cccgctgtgc
     2401 ttcttcgagt gacgtgatgc cctgtgccac ctttacgaat atctgttcct gcagcgtaag
     2461 catttttgtg gacttccgtg ccgtttcacg tatctgggcg atgttgccgg attcggcagc
     2521 ctgccgtatt gcgttatcga cctgcatgac ttcgaacgca gccgttcggc cgaaatatcc
     2581 gcattcgttg caggcggggc atggattata ttgcagttga cgtcgttcgt tctggtccgc
     2641 aggtattttg ggcgtacgat agaatttcgt gacgttagcc ggcagccgca ttttttcaag
     2701 cagttctggc gtagttggat attcttcccg acagttggtg cagattttac gcaggagaat
     2761 ctgtgatgtt accgctttta ccatatcgac actgccgccg agttttttcc atcgcaggat
     2821 agcctcggct gtttccgtgg ctggtatggc gattatgacg agtttgtttt tcgcgaaatc
     2881 gccgagaagt tccgcagatg ccgaatcgaa acagccgtca gccattatga cgtcggggtc
     2941 atgacgcaac acgttaacca gcgtctgacg atattcttcc ctgtcgttgt agacgtgttg
     3001 cgagacgttg gtcagttcgg cggcgggttt gtgttccacg gcgattatgt ttcgcaggta
     3061 agggtcgatt ttttttgtta tcgaatagag cgtgcttgta aggccggact tcggcaggcc
     3121 ggagacgata atcatgccgc tgccttcaag gcattcctga agaatggcga ggcttttcct
     3181 ggacaggccg agttcgtcaa ggttggtctg tacgacttca tttaatatgc ggagacgaat
     3241 tttatcgccc tggtttgagg ggcttatcgt ggcttcgacg tcgattttct tgccggccat
     3301 gtcgaatcct atcatgcctt tctgcggctt gtcggatttg tcgctcatgc cgccgatgtc
     3361 ttttatgaac gcggcaatgc ggtttacatc ttcggtatgc agcggttgct gtccgatggc
     3421 tatgccgtcg atgaggtata cgcatcttgc ccattcgccg gctgggtcga cggccgcttc
     3481 agctgcgcgt ccggcggcga cgctgaacag gaaatcctgc gtgagattgt agttcattac
     3541 gaacattgga ttggaaagtt tttcatcggt cagtattacc ggccggccgg cgttgtcgta
     3601 aagcggaatc ctggttcgtg cctccaattt atgcgatgtt ccgcctttga gacggctgaa
     3661 ccatgccgga gtgaatatgg cgaacgcctc attgaccctg ccgttgcgaa aaaccacata
     3721 tcccgcaaac gtgccgcctg caatcgccag aaacaggatt atccccacgg cgtaactgcc
     3781 gatgattatc cagatgaaca ggccaaaaac gccggcagcc agatatgccc cgctccatgc
     3841 cgcacgcggc agaagaaatc tgccggcatc cctgtccagc catactgtcg accacagcca
     3901 tatgcccgaa aacagaacgg caatcgccat tttaaccggc gaaccgtaca tgacaggttc
     3961 agttattgcg gaaattatgc tggcagccat aataaatacc tacgctttcg tcagtaaaca
     4021 taaaacacgg gacgacaaga cggcagatag catgatttta cgttttcttc gctcattgtc
     4081 gatagatgcc ggcttatttt tttcgccagg tcgtgccctg tggtgtatcg agtaactcta
     4141 tgttcatagc cgcaagttca tcacgaatgg cgtctgcgca gttaaagttt ttctgtcttt
     4201 tggcggcagt tcgttcggcg atgagcgact ctatttttgc gacgtcgagg ttttgggtct
     4261 gttcctgcat ctttgcgata ttcaggccca gcacttcgtc cattttgtaa agtgtggcca
     4321 gcacatctgc cggcgtggcg gcggaatcct ttgccgctcc ccacatagca gccagcgccc
     4381 tgggcatgtt caggtcgtcc atgacagcct gagtgaattt gtcgtaatat ccggcgatta
     4441 cgttgccggt tttgccggct gctgcagcct tgaggtccaa tacgtggcga gtgaggtttt
     4501 tgtaagcatt ggcggcggcg tcaaggcctt cgaaactgaa cgcaagctgc tgacggtaat
     4561 gcgcattgag caggaaataa cggtacgcca gcgggtcata accttttttt acaagtatgt
     4621 cgagtgttaa aaattcgccg gtcgatttgc tcatcttgcc gccgccggag tcgtcggatt
     4681 tggcgatggt cagaaattcg ccgtgcatcc accagttgca ccattcgtgg cccagcgtgc
     4741 tttcagcctg cgcgatttca ttagtgtgat gtatgtttac gtgatctatg ccgccgcagt
     4801 ggatgtccag ccgctcgccg agatatttta tggccatcgt tgaacactcg atgtgccagc
     4861 cgggaaatcc cacgccccac gggctgggcc attccatcag ccggtgttta tccgtcgggg
     4921 aaaatttcca caatgcgaag tcggttggat tgcgtttgtc gcccatttct atgcggatgc
     4981 cggcctgaag gcctttgacg tccagccggc ccaatttgcc gtagtcggac agtttgctgg
     5041 tgtcaaaata gattccgtcg gctgttcggt atgtgtagcc tttggcttcc agaattttga
     5101 cctgctcaat ctgttcggcg atgtggtcgg tagccttaca ccagacggtc ggctcgatta
     5161 tgttcagccg ggcaatatct tttcgaaacg cctgccaata aaaatcggct atttcccaaa
     5221 cggtcttgcc ttcgcgggca gcgccgaggg ccatcttgtc ttcgccctcg tcggagtcgc
     5281 tgactaggtg gccgacgtcc gtaacattca ttacgtgagt tacgtcgtag ccggcctgtt
     5341 tcagggcgcg cttcaggatg tcttcgaata cgtaagtccg cagattgccg atgtgggcga
     5401 aattgtaaac cgtcggcccg caggtataca acccgaccct gccgggagtt acaggtttga
     5461 actcgtccac actgcgcgtc attgtgttgt atagctttat catcgtctga aatccgtcag
     5521 cagcaattta agaacatgcg gaaagtcggt cggtttgaat caataaaaag gctggccggc
     5581 tatgcctttc gtaggttttt gtatatttca cgtttttttg cccagtcgtt ctgctctgcg
     5641 ggcttgtatt ttgttatttc gaacgaattc cgcataactt tgcggatgtc gtcgggtttt
     5701 ttaagtacgc ccatagcgag agcctggccc aggccgttgc ccagggcggt acactggtca
     5761 cagccggctt gcacttccat gccgcaggcg ttggcggtga actggcagag catcttgttc
     5821 tttatgccgc cgccgacgat gtatagtgcg tcaggccttt tgccggtgag ggtggcgatg
     5881 gaatctatgg ctgtattgta ctggagtgcc agactttcaa gtacgcatcg cacgagttcg
     5941 ccgcgggttt tgggtttcgg ctggccggtt ttttcgatga gagccattat cgcctcttcc
     6001 atatcgtgtg gcgccatcag cgaggtgtcc gccacgttta cgatggcgga gcttttggca
     6061 gtgccggctt ctgcggttat gcggttgtaa tcccacgact cttcgggtcg gtcccattta
     6121 cgtctgagtt cctgcaccag ccagaggccg atgatgttct gtgccagata ccatccgccg
     6181 atggtgtatt cgttcgtaaa accttctttc aggcatttgg gcgtagctac cggttcgtcc
     6241 accagtttgc cgagtatgct ccatgtcccg caactcagga atgcccagtt gttgccttcc
     6301 gccggcacag ccgccaatgc cgccgacgta tcatgtccgc acgtggcgac cacctccacc
     6361 ggccccatgc ctgtgagttt ctgcacttcc ggcgtgagcg taccgactac cgtcgcaggc
     6421 tcgaccagtt tgccgaacat cttcggcagg ctgaatgcgt ctatgatttc atcgcaccac
     6481 ttgccgtcct tgcccatgag gctggacgta ttggcgatgg acatttccga aatttttttg
     6541 cccgtgagca tatacgtgaa caggtcgggc atgttcagga agctgcctgc gaccttcagc
     6601 agcggagatt tatccctttt catggcgagc agttggaaaa gggaactgat agcccacggc
     6661 tcataggcgg tcagcttgaa aatctcgctt gtgtgcatcg tctggttgga gtattcgtgt
     6721 atgttttcgg tgcgggaatc acggtagcat acgcagttgc ccagcagttt gccgtctttg
     6781 ccgacgaggc cgaagtccac gccccacgta tcgacgctta tggatgttat gtcgattttc
     6841 ttttcggctg cgatggcgat tgcccgcagt atttcgtcga acaggtggag aaaatcccag
     6901 tacatcgtgc cggctgccgt tatgcggcga ttgggaaacc ggtgtatctc ttccatggat
     6961 acttttgcgt tgttcagcgt gactatgatt ccacggccgc tctcggcacc gagatctatc
     7021 gccaggaagt gctgtttttc agccatttat aaatactcct gtatgtttat gaaaagtggt
     7081 cagtcgggtt tgccggcttt ccacagggac tcaacttctt ttttagaaag catgcgcatg
     7141 tggccagggc cgaggccttt gaccgttagc gggccgatag ccgttcgctg gagttttttc
     7201 accttgtggc ccagccgcag cagcgttctg cgtatttcgc gatttctgcc ctcgcgcaag
     7261 gtcatttcga ttatggtatt ttcttggcct ttctgcaata ttttaacgtc ggcgccctgc
     7321 gttcgtctgc cgtccagata tacgccgtgc ctgatttttt caaaactctc gccatcaatc
     7381 gtgccgcgaa cggtgactat gtatgttttg ggaacttcat gactcggatg cgtcaggtgt
     7441 tgcgtaagtt cgccgtcgtt cgtcaacagt attgcgccgg tggaatcggc gtccagcctg
     7501 cctacgcagt atatccgctg ctttgcgaat tccggcggaa ggaggtctac tacgcacggc
     7561 cggccaagct ggtcgatggt ggtacagact acacccttgg gtttgttgag caggaaatac
     7621 gttttcgggg cggttttgcg gcggacgttt acgccgtcga cttttatgcg gtcttcgtcg
     7681 gggttgacga aaaacggcag ttccgtcacc acatgttcgt ttacgacaat tctgccttcg
     7741 acaatcattt cttcaattgt gcggcggctt gctacgccga gttcagcgag gtatttgtgc
     7801 agccgtactt tgcccgtcgg cgcaggaatg ccggcttttt cgttaggccg cgaacccaac
     7861 gatttctcca tacgtcgggc tgtcgtgttg cgattgttct gccgttgcgg ggctgaaggg
     7921 tcggcatttt tcgttttttt taaaatttta tgaggtatcg gcattttttg aaaatctgct
     7981 tctaaaaaaa gtgaatctct acgggttaaa acccgtaaca taatatttat aaggctcggt
     8041 tgtcagaatc attgtcgtgc cggcagggtg atttccagcc agtcggcgtc tgtttttgcc
     8101 tgaaggacag gctcttcaag tgtcgcaggc agaagtatgg tattgccggc ggttacctgc
     8161 gtcgtcggtt cggcatcgcc ggcatggttt atttcggcat cgcctccgaa aaacatcatg
     8221 gcgctgcata cgccggttgg cagcgtgaac ggctggccag cnnnnnnnnn ngccttcgtc
     8281 acgcggtgcg agacagtgaa atagtccgtt acccacagcg tatcgccggc tgcgccggag
     8341 gcgttatccg gcgtcggtgc gaaatgtatg cactgcatcg aacgttcgac gtgtatttct
     8401 ctgccgcggc cccagtcggt tacgcgatat gtcgtatcgg acggggtttg tacttccgct
     8461 atcacgaggc cggggccgac ggcgtgaacc gtgccggcgg gcaggtggta aaagtcgcct
     8521 tttctgcaat cgtatcgctt gacgt
//
LOCUS       DAONFI010000064         8524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9590_length_8524_cov_3.796906, whole genome shotgun sequence.
ACCESSION   DAONFI010000064 DAONFI010000000
VERSION     DAONFI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9590_length_8524_cov_3.796906"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..459)
                     /locus_tag="PLK08_03200"
     CDS             complement(211..459)
                     /locus_tag="PLK08_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPS52538.1"
                     /translation="MTRDEIKDKVVRAITDVLSLDSQEEEILRDENYSGGLTKWTSAT
                     HAEIIVAVEDAFDIEIDEMRIPGLNNVARIIEYIQQTV"
     gene            complement(456..1559)
                     /locus_tag="PLK08_03205"
     CDS             complement(456..1559)
                     /locus_tag="PLK08_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPS52539.1"
                     /translation="MSETQIIPVEIPRQTANDDTMFLLEWLVGDASQVRAGDAICKLE
                     SSKAAFEFSAPCDGFLHIISAAGKDLPVGETIAWITSSADRPGQWPSKPTHKQQHAAA
                     AIEGDLKITAKARSLMEKHNIADDAFANLSLVREKDVLEYLQKNPTTAVDGDKLQPHS
                     HLQLQTAKVLTKSFGTIPHSYLSKHVSAASLESAVEKICLKDDILISLSDLLVSTIAG
                     ILRDFPKINSTWLDAGLMLHREIALGVALNQNDGTLVVPVIHNADSLSLTEIAGKIRG
                     FQKRSIRNRLTVEDLTGGHFSVTTMIGTGVSQVFPIIYPEQAAILAIGDRWGCDDNEY
                     YTLTLAFDHRILNGAEAGCFLKAVCDRMLQEKK"
     gene            complement(1591..3558)
                     /locus_tag="PLK08_03210"
     CDS             complement(1591..3558)
                     /locus_tag="PLK08_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPS52540.1"
                     /translation="MSFSRKRLIEKMSEIRCFERKLLELFAAGRIYGTTHTCVGQEAC
                     AAALYANLDTSKDIAFSNHRCHGHYLSFGGTPERLFAEIMGRRGSICSGRGGSQHLCE
                     GHFYSQGIQGGSVPIAAGCAYYKKRMNCDGIVVAQIGDGTLGQGILYETLNIAGILQL
                     PLLIIVEYNGMAQSTETARTTSGDIVRRLGGFGIEVDRRSVSDPMELAEHFANVTAAV
                     RGGKPFIQVLDTFRLMAHSKGDDTRHPEIVKSGWKNDWLQKQLDEKDPDAMEAMAAAQ
                     KKIDLALKAIENSPEVELGSVATLASPDKPLFKNSADLLPRHDENTRVNQLLNHALDR
                     MLEKIPDVQLLGEDIVDDYGGPFKVTKGLSTKYPGRVFGTPISEQAIVGLANGWALMG
                     GHPIAEIMFADFVLLATDQIVNQAAKMHFMYDGKVNIPLTLRIVSGGYRGYGPTHSQC
                     TENFYANVPGLKVVALSQRHNPAALLQAAVLDPNPVILVENKTLYTQKPLATPPVGFE
                     FIATSPADDGEYPALYFSSASPQKSADVTLITYGGMTAMAEQAMLAMLMKDEIDFDYF
                     VLTELAPRNIDDILESVRRTSRVVFVEEGPADGGVGTEWLARLTEEISSPFQARRVGA
                     LPVPVPGARNIERKVLPDENRILSAILEILD"
     gene            complement(3820..4758)
                     /locus_tag="PLK08_03215"
     CDS             complement(3820..4758)
                     /locus_tag="PLK08_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HPS52541.1"
                     /translation="MNVKQHVINNIPPSAEFMLRCIKAAKDRYVPKKPKTSASVPQQP
                     CSAITTDDLRTILENLNIFPGDVLMLHSNSDAINRLGWTPTDMIDFLLEYLGPSGTLA
                     MPSHPKLKEEDGHKIYLPHRCPSYVGLVTELFRRRKQTLRSLYPFSAAAAQGERAEEL
                     IADHRHSFAPHDEKSPYAKLAVMKGKTLALGCALDRMTILHVAEDTMRDVMPIDDFYV
                     SEDVLIRTPNEEFTLRTHCRAPWLWWYLAKYQWTKHMYRQGFVRENCICGVPLREIDA
                     TAAIEWMQQRVRKGKTIYPYAKMNRFFHFKNMPGKE"
     gene            complement(4755..5771)
                     /locus_tag="PLK08_03220"
     CDS             complement(4755..5771)
                     /locus_tag="PLK08_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52542.1"
                     /translation="MASDDLGVTFREIGQIPILSKYAPVAKFSKLARRVLRMSTYRLR
                     VLDGGNIIFTFKGGVYLLRAGEKQAVCQFVIERGSRPVSLAYKPGGMVIWGEYFDNRE
                     RGPVNIYGSCDLGETWQKVYSFNGEIRHVHGITYDEYADCFWICTGDREGEERLIRAN
                     ADFSKVDVVLKGGQKNRFYSLTVTPDVIWTANDSPNCDNFVRRYDKSTGEIDDIARID
                     NSSFYGCMVGERYFCSTNSERPERNLPRTFSQPNDYSATHVWMTNSRTSQARRVLSFP
                     TDIWFKLSGLPKVHPVLFQYTRVFFPEGHNPTDKLVCHTIGTGGCDDCMLVYDISALE
                     AAGL"
     gene            complement(5951..6637)
                     /locus_tag="PLK08_03225"
     CDS             complement(5951..6637)
                     /locus_tag="PLK08_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPS52543.1"
                     /translation="MAFTLKDVVPWGRSFDEYVAMFALTEDDLKKRILGCGDGPAAFN
                     CTLTNRGGSIVSVDPIYRFSAEEIKNRINETYVQVMEQTEQNRHEFVWENISSPQELC
                     ELRIAAMNDFLSDYPAGLANGRYIDASLPNLPFADKTFELSLCSHLLFLYSEHLTADF
                     HIMVIKELCRVAGEVRIFPLLELGAKKSRHLDTIINSLQNDSFTVNIRKVPYEFQNGG
                     NEMLVAEANH"
     gene            complement(6732..7976)
                     /gene="hydF"
                     /locus_tag="PLK08_03230"
     CDS             complement(6732..7976)
                     /gene="hydF"
                     /locus_tag="PLK08_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPS52544.1"
                     /translation="MQTTPKGFRLQIGLFGRRNVGKSSLLNAMTRQSVSIVSDVAGTT
                     TDPVEKPMELLPIGPVLFIDTAGIDDVGALGEMRVAKTRKIFDRTDVGIIVCAAGEWG
                     EFERKILAELVSRKTPVIIAANKSDLAGPTADILDNAKADGITLVSTSATSGQGILDL
                     REALIKAAPEEFINAPTIVGDLLPAGELAVLVVPIDLEAPKGRLILPQVQTIRDILDT
                     DAYCMMVKERELRDALERLNRPPAIVITDSQAFLKVSADTPPAVPMTSFSILFARNKG
                     DLGEFVRGAMAIENLKPGDRVLIAEACSHHPIGDDIGRVKIPRWLRQYVGGDIQFDSV
                     QGHDFPDDPAELGRYKLVIHCGGCMWNRREMLSRIMHCRRAGVPITNYGITIAYTLGI
                     FERALAPFPAAMEEYRKIKNSN"
     gene            complement(7979..>8524)
                     /locus_tag="PLK08_03235"
     CDS             complement(7979..>8524)
                     /locus_tag="PLK08_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPS52545.1"
                     /translation="SNLLKVSGDLRLISSGPEAGLGEIALPAMQAGSSIMPAKVNPVI
                     PEAVSQAAICVFSNDQAVTTACSLGSLELNAFMPLIADSLLGSIDLLTKACALLREKC
                     VAGITPNRAACQAHVQGSTASVTALLEKIGYEKAVQLAADAKAKNKTIRQAAVDSGLL
                     SEKEFDELTSPQHVTQLGSRK"
BASE COUNT         1747 a         2169 c         2298 g         2310 t
ORIGIN      
        1 ttttgcagcc tggcggcgtc agaaaaacgg cagaatattt ttttaaaaaa gtatgttgcc
       61 gttcttcttt cttgccagcc cgcaaaattg ctcgatttcg gcgacgccca tattttgggg
      121 ataggttcga aaaaaactca aaataaccga tgttcgtcgg attttctgag tggaaagtgt
      181 ggatataaca cagcttttca gttattcgct tcagacggtc tgttgtatat attcgataat
      241 tctggcgacg ttgttaaggc cggggattcg catttcatca atctcaatgt cgaaagcgtc
      301 ttcgacggct acgattattt cggcatgggt tgctgatgtc cactttgtaa ggccgccgga
      361 gtaattctcg tctcgaagaa tttcctcttc ctgtgaatca agggaaagta catccgtgat
      421 ggcccgtaca accttgtcct ttatttcatc gcgggtcatt ttttctcctg taacatcctg
      481 tcgcagacag ccttcagaaa acagccggct tccgcgccgt tgagtattct gtggtcgaag
      541 gctaatgtca gcgtataata ttcgttatca tcgcagcccc acctgtcacc gatagccagg
      601 atggctgcct gttcgggata tattatcggg aatacctggc tgacgccagt gccaatcatg
      661 gttgttacgg aaaaatgtcc gccggtgagg tcttccaccg tcagccggtt tctgatggag
      721 cgtttctgaa agccgcggat tttgccggcg atttcggtca ggctgagcga atcggcgttg
      781 tgaatgaccg gcactacgag tgtgccgtcg ttctggttca gggctacgcc gagggctatt
      841 tccctgtgca gcatgaggcc ggcgtccagc cacgtggagt ttatttttgg gaaatcccgc
      901 aaaattccgg ctatggtgct taccagcagg tcgctgaggc ttatgaggat gtcgtcttta
      961 aggcagattt tttcgacagc cgactcaagg cttgccgctg agacgtgttt tgaaagatac
     1021 gaatgcggaa tcgtgccgaa agattttgtg agaactttcg ccgtctgaag ttgcagatgc
     1081 gaatgcggct gaagtttatc gccgtcgact gcggtcgtcg gattcttttg cagatattca
     1141 aggacgtctt tttcccgcac cagcgataag ttggcgaagg cgtcgtcggc aatattgtgt
     1201 ttctccatca aactgcgggc tttggcggtt attttcaaat cgccttcgat ggcggcagcg
     1261 gcatgttgct gtttatgcgt cggctttgac ggccattgac cggggcggtc ggctgaagat
     1321 gttatccatg ctatcgtttc gcccactggc aggtctttgc cggcggctga gattatgtgc
     1381 aaaaaaccgt cgcacggcgc cgaaaattca aatgccgcct tgctcgactc cagtttgcat
     1441 atggcatcgc cggcgcgtac ctgcgaggcg tcgccgacca gccattcaag gagaaacatc
     1501 gtgtcgtcgt tagctgtctg tcgcggtatt tctacgggta tgatttgcgt ttcgctcatt
     1561 tcagatatgt cctcaataaa ttgtccgcgt ttaatccagt atttccagaa tcgcagacaa
     1621 tattctgttt tcgtccggca ggacttttcg ttcgatattt ctcgcacctg gtaccggcac
     1681 gggcagagcg cccacccgcc gcgcctggaa cggcgacgaa atttcttccg tcagcctcgc
     1741 cagccattca gtgccgactc cgccgtctgc cgggccttcc tccacgaata cgacgcggct
     1801 ggtacgtcgg acggattcaa ggatgtcgtc gatattgcgt ggcgcaagtt ccgtcaatac
     1861 gaaataatcg aaatctattt catccttcat cagcattgcc agcattgcct gctcagccat
     1921 tgcggtcatg ccgccgtagg ttatcagcgt aacgtcggcg gatttttgcg gcgatgccga
     1981 tgagaagtac agcgccggat attctccgtc gtctgccggt gatgttgcga tgaattcaaa
     2041 gccgaccggc ggcgtcgcga gtggtttttg ggtatacagc gttttgtttt cgacaaggat
     2101 taccgggttg gggtcgagta ctgccgcctg cagcagggct gcggggttgt gtcgctgcga
     2161 gagggctacc actttcagac cgggtacgtt tgcataaaag ttttccgtac actggctgtg
     2221 cgtcgggccg tagccgcgat atccgccgga aacgattcgc aacgtcagcg ggatgttcac
     2281 ttttccgtcg tacatgaagt gcattttggc ggcttggttg acaatctggt cggttgccag
     2341 caggacgaaa tcggcgaaca tgatttccgc tatcggatgg ccgcccatga gcgcccagcc
     2401 gttggccaac ccgacgatag cctgctcgga tatcggcgtg ccgaagaccc tgcccggata
     2461 tttcgtcgaa agacccttgg tgaccttgaa cggcccgccg taatcgtcga ctatgtcttc
     2521 accgagcagt tgtacgtccg gaattttttc aagcatgcgg tccaatgcat ggttgaggag
     2581 ctgatttacg cgggtatttt catcatgccg cggcagcagg tcggcggaat ttttgaataa
     2641 cggcttatcc ggcgaggcta gcgtggcgac agaccccaac tcgacttccg gcgaattttc
     2701 gattgccttc agggcgaggt ctattttctt ttgtgccgcc gccattgcct ccattgcatc
     2761 ggggtctttt tcgtcaagct gtttctgcag ccagtcgttt ttccagccgc tttttacaat
     2821 ttccggatgc cgtgtgtcat cgcccttgct gtgagccatc aggcggaacg tgtcgagtac
     2881 ctgtatgaac ggtttgccgc cgcgcacggc agcggttacg tttgcgaaat gttccgccag
     2941 ttccatcggg tcgcttacgc tgcggcggtc tacttctatg ccgaatccgc ccagccgcct
     3001 gactatatcg ccgctggtcg tgcgagccgt ctccgtcgac tgggccatgc cgttgtattc
     3061 cacgattatc agcagcggca gttgcagaat accggcgatg ttgagcgttt cgtagagtat
     3121 gccctggccc agagtgccgt cgccgatttg cgccaccacg atgccgtcgc agttcatgcg
     3181 ttttttgtaa tatgcacagc cggcggctat cggcacgctg ccgccctgga tgccctgaga
     3241 atagaaatgt ccttcgcaca gatgctggct gccgccgcgg ccgctgcaaa ttgaaccgcg
     3301 acggcccatg atttccgcga aaagtctctc cggcgtgccg ccgaacgaaa gataatggcc
     3361 gtggcagcgg tgattgctga atgcgatgtc tttcgacgtg tcgagatttg catacaatgc
     3421 tgcggcgcat gcctcctggc cgacgcacgt atgcgtagtg ccgtaaatgc ggccggctgc
     3481 gaaaagctcc agcagcttac gctcaaaaca gcgtatttcg gacatttttt ctataagtct
     3541 ttttcttgaa aacgacatca aactctccat cagtcagttt gaattttttt taacgacaat
     3601 tttttaaata ttatttacat ggcgtatttt tttaagttgt gattttaaaa ttattttttg
     3661 tctggcgtat tattaaaagt tgcgattaaa attttttttg tttggctcat tttagaaagt
     3721 tacgattaaa aagttatttt ttgcttggtt cattttaaaa agttacgatt aaaaagttat
     3781 tttttgcttg gttcatttta aaaagttgct attaaaaaat tattcttttc ctggcatatt
     3841 tttaaagtgg aaaaacctgt tcattttcgc atatgggtaa attgttttgc cttttcgtac
     3901 ccgctgctgc atccactcga ttgccgccgt cgcgtcaatt tcccgcagcg gtacgccgca
     3961 tatgcaattc tcgcggacga agccttgccg gtacatgtgt ttcgtccact ggtattttgc
     4021 cagataccac cacagccatg gcgcccggca gtgcgttcgg agcgtgaatt cttcgttggg
     4081 cgttctgatg agtacatctt cactgacgta gaaatcgtct atcggcatga cgtcgcgcat
     4141 ggtatcttcg gctacgtgga ggattgtcat cctgtccagg gcacagccca gtgccagcgt
     4201 tttgcctttc attacggcaa gtttggcgta aggagatttt tcgtcgtgcg gcgcgaacga
     4261 atgacgatgg tcggcgatga gttcttccgc gcgttcgccc tgtgcagccg ccgcgctgaa
     4321 cggatacaga ctgcgcagag tctgttttcg acgacgaaac agttcggtga caaggcctac
     4381 gtatgacggg cagcggtgag gcagatagat tttatggccg tcttcttctt tgagtttcgg
     4441 gtgcgacggc attgccagcg tgccggacgg gccgagatat tccagcagaa aatctatcat
     4501 atccgtcggc gtccagccga gcctgttgat tgcatctgaa tttgaatgaa gcatcagcac
     4561 gtcgcctgga aatatgttca ggttttccag gatggttctc aggtcgtcgg ttgttattgc
     4621 gctgcagggc tgttgaggta cggaggctga ggtttttggt tttttgggga cataccggtc
     4681 tttggcggct tttatgcagc gcaacatgaa ttctgcactg ggcgggatat tgtttatgac
     4741 atgctgtttg acgttcataa tccggcagcc tccagggcgc ttatgtcata taccagcatg
     4801 cagtcgtcgc agccgcctgt gcctattgtg tgacagacga gtttgtcggt cggattgtgg
     4861 ccttccggaa aaaagacgcg agtatactgg aacaacacgg ggtgcacttt cggcaggccg
     4921 ctgagtttga accatatatc cgtcgggaac gacaatacgc ggcgtgcctg actggtacgc
     4981 gagttagtca tccacacatg agtggcggaa taatcgttcg gctgtgaaaa tgtccgcggc
     5041 aagttacgtt caggccgctc agaattcgtg gaacagaagt atctctcgcc gaccatgcag
     5101 ccgtaaaaac tcgaattgtc tatcctggcg atatcgtcaa tttcaccggt tgacttgtca
     5161 taacgacgga cgaaattgtc gcaattcggc gaatcgtttg ccgtccatat cacgtccggc
     5221 gtgacggtca gtgaataaaa acggtttttc tggccgcctt tgagtacgac gtccactttt
     5281 gaaaaatcgg cattggcgcg gatgaggcgt tcttcgcctt ccctgtcgcc ggtgcatatc
     5341 cagaagcagt ctgcgtattc gtcgtaggta atgccgtgaa cgtgacgtat ttcgccgttg
     5401 aacgaatata ctttctgcca tgtttcgccc aggtcgcaac tgccgtagat attgaccggc
     5461 ccgcgttccc tgttgtcgaa atattcgccc cagattacca tgccgcccgg tttatacgca
     5521 agcgacacag gccttgagcc ccgctctatg acaaactgac agaccgcctg tttttcaccg
     5581 gcacgcagca gatatacgcc gcccttgaac gtgaaaatta tattgccgcc atccagcacg
     5641 cgaagacgat acgtactcat gcgcagaact cgacgggcca gcttggaaaa tttcgcaacc
     5701 ggagcatatt tggataaaat cggaatttgg ccgatttcgc ggaacgttac gccgaggtcg
     5761 tcgctggcca atatggaatc gtatcgcgaa caatagagtg tattgccagc gaaaaaaaga
     5821 ggccgcacac ctcggatttt tcttatatat ctgggattca tgtacctgct ccgcaaaatt
     5881 tgtattatcc aaactttttt ttataaaaca agttatgacc caagaaattt ggcttaatta
     5941 caaacgtttg ttaatggttg gcctctgcta ccagcatttc atttccgccg ttctgaaatt
     6001 cgtatggaac ttttcgtatg tttacggtga aactatcatt ttgcaggcta tttattatgg
     6061 tgtcgagatg tctggatttt tttgccccaa gctccagcag tggaaatatg cgcacttcac
     6121 cggcgacacg gcagagctcc tttatcacca tgatgtgaaa atccgccgtc agatgttcgc
     6181 tgtaaagaaa cagcagatgc gaacatagcg aaagttcaaa tgttttatcc gcaaaaggca
     6241 gattcggcag actggcgtca atataacggc cgttagccag gccggccggg tagtcggaca
     6301 gaaaatcatt catggcggct atgcgcagct cacacaattc ctgcggtgat gagatgtttt
     6361 cccatacgaa ttcgtgtcgg ttttgctctg tctgttccat gacctgcacg taggtctcat
     6421 tgatgcgatt tttgatttcc tcggcgctga aacgataaat tgggtctact gacactatgc
     6481 tgccgccgcg gtttgtcagt gtgcagttga aagccgccgg cccgtcgccg cagccgagaa
     6541 tgcgtttttt caggtcatct tccgtcaggg cgaacatcgc gacatattcg tcaaagctgc
     6601 gaccccacgg aacgacgtct tttaatgtga atgccataaa aacgccctcc gtaaaactta
     6661 ttttttagct caaagtgacc gattgacgat atttaccttt atgcctggta atccggaaac
     6721 cgttaaatcg gtcagtttga atttttaatt ttgcgatatt cttccattgc agccggaaat
     6781 ggggctagcg cgcgttcgaa tatgcccagc gtgtatgcta tggttattcc gtagttggtt
     6841 atcggcacgc ctgcgcggcg gcagtgcatt attcggctga gcatctcacg acgattccac
     6901 atgcatccgc cgcagtgaat tacgagtttg tacctgccca gttcggcggg gtcgtcagga
     6961 aaatcgtgac cctgcacgct gtcgaactgt atgtcgccgc cgacgtactg tcgcagccag
     7021 cgggggattt tgacgcgccc gatatcgtcg ccgatgggat ggtgcgagca ggcttcggct
     7081 atcagcacgc ggtcgcctgg tttgagattt tctatggcca tcgcgccgcg gacgaattcg
     7141 ccgaggtcgc ccttgtttcg tgcgaagaga atcgaaaaac tcgtcatcgg cacagccggc
     7201 ggcgtatccg cagaaacttt cagaaacgcc tgcgaatcag taatcacgat tgccggcgga
     7261 cgattcagcc gctcaagtgc gtcccgcaat tctcgctcct tgaccatcat gcaatatgcg
     7321 tcggtgtcaa gtatgtcgcg gatggtctgt acttgcggga gtattaaacg gcctttgggg
     7381 gcttcgaggt ctatcgggac taccagcacc gccaattcgc cggctggcag aaggtcgccg
     7441 acgatggtgg gggcgtttat gaactcttcg ggagcggctt tgatgagggc ttcacgcagg
     7501 tcgagtatgc cttggccgct ggttgcggaa gtgctgacga gagttatgcc gtcggctttc
     7561 gcattgtcga gtatgtcggc ggtcggcccg gcgaggtctg atttattcgc tgcgataatc
     7621 accggcgttt tgcggctgac aagctcagcc agtattttcc gctcgaattc cccccattcg
     7681 cccgcagcgc agacgattat gcccacgtca gtgcggtcga agattttgcg cgtttttgcg
     7741 acgcgcatct cgccgagcgc gccgacgtca tctatgccgg cggtgtctat gaacagtacc
     7801 ggcccgatgg gcagaagctc catcggcttt tccacagggt ctgtcgtcgt gccggcgacg
     7861 tcggagacta ttgacacgct ctgtcgcgtc attgcgttga gcagcgagct tttgccgaca
     7921 ttccgccggc cgaaaaggcc tatctgcagc cggaatcctt tgggcgttgt ttgcattttt
     7981 atttccttga ccccaactgc gttacatgtt gcgggctggt gagttcgtcg aactcttttt
     8041 cgctgagcag gccgctgtcg acggcggcct ggcggatggt tttgtttttc gcctttgcgt
     8101 cggcggctaa ctgtacggct ttttcgtagc cgattttttc caacagtgcc gtcacgcttg
     8161 ccgtcgaccc ctgtacgtga gcctggcatg ccgcgcggtt cggcgttatg ccggcgacgc
     8221 atttttcccg cagcaatgcg catgctttcg tcaaaaggtc tatgctgccc agcagcgagt
     8281 cggcgattaa cggcatgaag gcgttaagtt caaggctgcc cagcgagcag gctgtcgtga
     8341 cggcctggtc gtttgagaat acgcagatgg ctgcttgcga gacggcttcg gggattacgg
     8401 gattgacttt tgcgggcatg atactgctgc cggcctgcat agctggcagg gcgatttcac
     8461 ccaagccggc ttccggcccg ctcgatatca gacgcaaatc gcccgatacc ttcagaagat
     8521 tgct
//
LOCUS       DAONFI010000065         8496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9631_length_8496_cov_4.837578, whole genome shotgun sequence.
ACCESSION   DAONFI010000065 DAONFI010000000
VERSION     DAONFI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9631_length_8496_cov_4.837578"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="PLK08_03240"
     CDS             <1..157
                     /locus_tag="PLK08_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPS52546.1"
                     /translation="VRTIVSMGHALNRRVIAEGVETEEQVAILNSNGCDEIQGYWFSR
                     PLREISN"
     gene            229..621
                     /locus_tag="PLK08_03245"
     CDS             229..621
                     /locus_tag="PLK08_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52547.1"
                     /translation="METSPSAQIYVRNLAEPADSLGKLTAGTMALAQEFDGWWSIFVL
                     PPRSVCFIAKIEESPGGNSYLVREQTASSGGGLTTVIDSAQLPACNVAEMVTPASTPV
                     AVGTYVTVFGVADTGYPARLHYFFNHQV"
     gene            648..914
                     /locus_tag="PLK08_03250"
     CDS             648..914
                     /locus_tag="PLK08_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52548.1"
                     /translation="MGNIQELTNFGAAGLMGAMWLIERKLSRTREEQLAEAHKRICRD
                     EEKLSCLTDVINRNTAAIVRLVQNQNYQCELLQHLLEEFHHKPV"
     gene            925..1410
                     /locus_tag="PLK08_03255"
     CDS             925..1410
                     /locus_tag="PLK08_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52549.1"
                     /translation="MKRILLGLAVSMMCCGCTTPTATTELISQGIKGLELARSAREQS
                     YTIETKMYQQQLASLNTAFDADVSLVSAGGLKDKDGKAVEMTAEWIISARKGYSAAVD
                     SINEKMVKAQQVYSTDLDNITAANEALQLANELVVMQWNVAEKFKEQFLKLQQENTKN
                     E"
     gene            1403..1657
                     /locus_tag="PLK08_03260"
     CDS             1403..1657
                     /locus_tag="PLK08_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52550.1"
                     /translation="MNETDMKTIVAQAVAEAFDTWAQSHPSLAAVIDKALLMERTTES
                     LRQSDEYKQAIKDYADSREEMNILGNLVDLAEQILPLLIA"
     gene            1673..1927
                     /locus_tag="PLK08_03265"
     CDS             1673..1927
                     /locus_tag="PLK08_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52551.1"
                     /translation="MKDFLTRISSRKFLTAFAVEIAAVAALFSPERASEFNDAAIRIA
                     AIVTMALTALGYGCIEASLDNSSSGSSTDETAEESQNDAQ"
     gene            complement(1883..2839)
                     /locus_tag="PLK08_03270"
     CDS             complement(1883..2839)
                     /locus_tag="PLK08_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPS52552.1"
                     /translation="MNVLNTLFPLFFLIALGAILRWRNITGDLMIKDLNRLVFWIGLP
                     SLLFVKVATANSQLDSRSLGVLWVLLLATGATAVIAFIFCIIFRFDGEKAGPFIQGAF
                     RGNWAYIGLTVIFFAFANSPDKIRATANDTAALVIGPIVAIYNIASIIILLAGKHSLN
                     ARAFKRMFINMLVNPLLLACIFGIAWNLFKIPLPVFLSRSLETAGNFALPVALICVGG
                     SLMQTKIKGFLLNPAILSAVIKVFICPLIGYFIARWMGIGGVETMVALFLLATPTAVN
                     SFTVADQLGCDSLIAAEIVIVSTTMSIISLGIVLGLFSSFVR"
     gene            complement(2936..3499)
                     /gene="efp"
                     /locus_tag="PLK08_03275"
     CDS             complement(2936..3499)
                     /gene="efp"
                     /locus_tag="PLK08_03275"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPS52553.1"
                     /translation="MAKKVNDFKKGQGCIWKNNEIWVFLKMEWVKPGKGPSYIQAELR
                     NAKTGQIVANRFRPEETLEPVHFDIKKYEYLYTDANSHILMDPETYDQIELPLDVIGD
                     NKVYLTENCEIEVCSVDGKYISAELPYTVELKIVDCPPNVKGGTVTNVLKEAVCEGGA
                     KIRVPGFVEVGEMIKVDTRTGEYLGRV"
     gene            complement(3521..4363)
                     /locus_tag="PLK08_03280"
     CDS             complement(3521..4363)
                     /locus_tag="PLK08_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52554.1"
                     /translation="MLATAADYFFLPWEIPIWVIISFVSLMIGGYFISTGIQKTGIVK
                     KMPLGMGILAMFLIGSIAAFFGIIIYRVGMVASASSISYGGIIGTAVTFAGMFYLLMW
                     TWQKKLSAGEIFHICLYPLASTFVLAAVIGTPTLMIAYNTVQKNARESVLVNQTMHNL
                     QDIFFALTQRGVDRPPASLDVLVKEKLITADKLKSPANADRLIGFIYAPIDISYANRE
                     ENQDKIIACDYKDNFDNYRVVLYANGKLQRLSEASFQDSLQQPVNKQLKQIVQKLETP
                     QTGK"
     gene            complement(4518..5798)
                     /locus_tag="PLK08_03285"
     CDS             complement(4518..5798)
                     /locus_tag="PLK08_03285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPS52555.1"
                     /translation="MKTAADTARRKISEFCGEDYIFGCGCFDRIGEHFAVLGRRAAVI
                     VSGFGREWGSRLHARLRTSFEAAGVEILGELIPAAAENSPFEDVMRITDEIRRRDPDF
                     VVAVGGGSNLDAAKAANVHAVFGDMCGMYDYFGVGKISELIEKTGRKLRPFAAAELAS
                     ASGSHLTKYSNITDLTVGQKMLIIDDAITPTRAIFDYNLTVTMPRDFTLDGAFDGLAH
                     CLEVWMGIPDAAKVRDVALAGIDLIVSNIEKLTENPSDSHLREMLGLGTDCGAISIMT
                     GGTNGAHLSSFSLVDVMPHGRACALLEPYFVVFFAPVIEDRLRPVAEIYRRAGFLKEN
                     PDVLAGVELGLAVAEAMVCQSKKLGFVTKFSDVPKFGELHIKRILSAAKKPALASKLQ
                     NMPLPMSADMVDEYLGAVLKAAQTGDFSIIKHVE"
     gene            complement(5795..6646)
                     /locus_tag="PLK08_03290"
     CDS             complement(5795..6646)
                     /locus_tag="PLK08_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPS52556.1"
                     /translation="MNIFFNALVNEPFLQIALLGGLMVSIACGVVGSYVVVRRISYIA
                     GGIAHCVLGGIGAAYYLNNVCGWAWLDPIYGAVAAAVAAAIIIGLVSLHARAHEDTII
                     GGMWAIGMAVGVMFVSATPGYSQDLMSYLFGNILLITAKDIYLICILDVLVLGVGLTF
                     YRQLLAVCFDEQFARARGMKVHWWFLLLLIVTSLTVVLLSTVVGIVMVIALLTLPAAI
                     ARMFTRRLWSMMLAATAICAGFIIAGFAASYQPELPTGATIIIIAGSVYLLCVTCKSL
                     AMRGKSS"
     gene            complement(6643..7410)
                     /locus_tag="PLK08_03295"
     CDS             complement(6643..7410)
                     /locus_tag="PLK08_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPS52557.1"
                     /translation="MAIAIEVKNLDFGYDSSQLVLQDVNFHIESGDFVSIVGPNGGGK
                     TTLVLLLLGLLEPTRGDIRILGLPPYEARGKIGYLAQRIALDMNFPVSVLDVVRMGCA
                     DRAGFFGVFGGEDRNAAIEALAAVGMSKFAGRNFSALSGGQRQRVLIARAIAPKPEML
                     LMDEPAASLDVQVGSEFYELLGRLNKDMTIITVSHDMAFVSEYVKTVLCVHGRVAVHP
                     TEKWDERMMDELYGGPVRRVRHEHNCVEKGCEGSHER"
     gene            complement(7394..8311)
                     /locus_tag="PLK08_03300"
     CDS             complement(7394..8311)
                     /locus_tag="PLK08_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPS52558.1"
                     /translation="MKKSSVIAILFCLLAGGCKNSTTSQNNGKLDVFVSIEPDRFLVE
                     HIGGSRVSVSVLVPAGREPHDYEPTGRQISQLGRAAVFFRTGVPFEEALIPRIRELFP
                     KLPIIDLRRGITLLPMSRDDEEHERHEGHEHGMLDPHIWMSPELMITQALTVRDELVK
                     LDPADAELYETNCSRLVADLTAVSHRIAKLLAPYKGRMFFVFHPTFGYFAHEFGLTQE
                     AVEVEGKSPTAAQLQQLIDNARRQNVRIIFVQPQFSRHAAQSIANAIGGAVIPLDSLS
                     ADYLKNLNKVADEIAAALGGEKPLNGHCD"
BASE COUNT         2172 a         2285 c         2046 g         1993 t
ORIGIN      
        1 cgtgcgtacc atcgtcagta tgggccacgc cctgaaccgg cgggtcatcg ccgagggcgt
       61 cgagaccgag gaacaggtgg cgatcctcaa cagcaacggc tgcgacgaga tccagggata
      121 ctggttcagc cggccgctgc gagagatatc aaactgattc gcataagcac cgttaccatt
      181 cacaacgttt attatgccga agcgtttcgc ctgaactcgg acggcaacat ggaaacatct
      241 ccgtcggcgc agatatacgt tcgaaatctg gcagagccgg cagattcgct cggcaagctg
      301 acggctggaa caatggcctt ggcgcaggaa tttgacggct ggtggagcat attcgtcctg
      361 ccgccgcgat cggtatgttt tatcgccaaa atagaagagt cgccaggcgg aaattcgtat
      421 ctcgtgcgtg aacagactgc ctcttccggc ggagggctca cgacagtaat tgactcggca
      481 caacttcccg catgcaatgt cgccgaaatg gttacgcctg catcgacccc tgtagccgta
      541 ggaacttatg tcacggtatt cggcgtagca gataccggtt atccggcacg actacattat
      601 tttttcaacc atcaagtctg aaagcatctg ccatgtatga aatgattatg ggaaatattc
      661 aggaacttac gaattttggc gcggctggcc tgatgggtgc aatgtggctg atagaacgca
      721 aactcagccg aacccgtgaa gagcaactgg ccgaagccca taaacgaatc tgtcgtgacg
      781 aagaaaaact cagctgtctg acagacgtta taaatcgaaa taccgccgca attgttcgcc
      841 tggtgcagaa tcagaactat cagtgcgaac tgcttcaaca cctgctagaa gaatttcatc
      901 acaaacccgt ttaaggagaa aatcatgaaa agaatactgc taggattagc ggtgagtatg
      961 atgtgctgcg gatgcactac tccaaccgct acgactgaac tcatctcgca aggcataaaa
     1021 ggccttgaac tggcccgaag cgcacgtgag cagtcgtaca ccattgaaac aaaaatgtat
     1081 cagcagcaac tcgcttcgct taatacagcg ttcgatgcag acgtctcgct tgtttccgcc
     1141 ggcgggctga aagacaagga cggcaaagcc gtcgaaatga cggcggaatg gataatttcg
     1201 gcaagaaaag gatactctgc cgccgtcgac tcaatcaacg aaaaaatggt caaggctcag
     1261 caggtatatt ctaccgatct ggacaacatc acagcggcaa acgaggcttt gcagctcgcc
     1321 aacgagctgg tcgtcatgca atggaacgtc gctgaaaaat tcaaggaaca gtttctgaaa
     1381 cttcaacagg agaatacgaa aaatgaatga aacagacatg aagacaatcg tggcacaggc
     1441 tgtggcggaa gcgttcgata cctgggcgca gtcgcatccg tcgctcgccg cagttataga
     1501 caaggctctg ctgatggaac ggacaacgga atccctgcgg cagagcgatg aatacaagca
     1561 ggctatcaag gattacgccg acagccgtga agaaatgaac attttaggta acctcgttga
     1621 cctggcggaa cagattctgc ccttattaat cgcgtgaaag gaaacgtaaa tcatgaaaga
     1681 ttttctgacc agaatatcgt caaggaaatt tttgaccgcc tttgcggtcg aaattgcagc
     1741 cgtcgcggca ttgttttctc cggaacgcgc atcggaattc aatgacgcag caatacgtat
     1801 cgccgcaata gtgacaatgg ctcttaccgc gctgggctat ggctgcatcg aagccagtct
     1861 ggacaacagc agcagtggtt cgtcaacgga cgaaactgct gaagagtccc aaaacgatgc
     1921 ccagtgatat tatggacatg gttgtactga cgatgactat ctcggctgcg atgagactgt
     1981 cgcagccgag ctggtcggct acggtgaatg aatttaccgc cgtcggagtg gctagcagaa
     2041 aaagcgcaac catcgtttcc acgccgccga ttcccatcca tcgcgctatg aagtaaccga
     2101 taagcggaca gataaacacc ttgattacgg ctgataatat cgccggattc aacagaaaac
     2161 cctttatctt tgtctgcatc agtgacccgc ctacgcatat gagagctacc ggcagggcaa
     2221 aattgccggc agtctcaaga ctgcgagata agaaaaccgg cagcggaatt ttaaacagat
     2281 tccatgctat gccgaaaatg cacgccagaa gcagcggatt gacaagcata tttatgaaca
     2341 ttcgcttaaa tgctcgcgca ttgagcgagt gtttgccggc aagaagtatt ataatgctgg
     2401 cgatattgta aattgcaact atcgggccta tcacgagagc ggcggtgtcg ttggcagtag
     2461 cacggatttt gtcaggtgaa tttgcaaacg caaagaaaat aacagtcaag cctatgtacg
     2521 cccaatttcc acgaaacgca ccctgtatga atgggccggc tttttcacca tcaaatctga
     2581 aaatgataca aaaaataaaa gcaataactg cagtcgctcc cgtcgccaac agcaaaaccc
     2641 aaagcactcc cagtgaccgc gaatcaagct ggctgtttgc cgtggcaacc ttaacgaata
     2701 gaagactcgg caaacctatc cagaacacca gtcgattgag atcttttatc atcaaatcac
     2761 cggttatgtt tcgccatctc aatatcgcgc ccagggcgat gagaaaaaat agtggaaaca
     2821 gtgtgttgag aacgttcatg caaaaatgtc cggcttgcaa ttaacggagg ctcgccgaaa
     2881 tgacgagcct ccactttttt tactttttac gaattagtct gttttagaga accttttaaa
     2941 ctctgccgag atattctccg gtgcgggtat cgacttttat catttcgccg acttccacga
     3001 aacctggcac acggattttc gcaccgcctt cgcagacggc ttccttgagc acgttcgtga
     3061 ccgtaccgcc cttaacgttc ggcggacaat cgacgatttt caactcaaca gtatacggca
     3121 gttcagcgga aatatatttc ccgtccacgc tgcagacttc tatttcgcaa ttttccgtga
     3181 gatagacttt attgtcgccg ataacatcca gcggcagttc tatctggtcg taagtttcag
     3241 ggtccatcaa aatatggctg ttggcatcag tatagagata ttcgtatttt ttgatgtcga
     3301 aatgaaccgg ttcaagcgtt tcttcaggac gaaacctgtt tgccactatt tgtcccgttt
     3361 ttgcatttcg taactcagcc tgaatatagc ttggcccttt gccgggcttg acccattcca
     3421 ttttcaggaa tacccagatt tcattgtttt tccagataca tccctggcct tttttaaaat
     3481 cgttaacttt cttcgccatg caactaatct ccaatattta ttattttccc gtttgcgggg
     3541 tttccaattt ttgtactatc tgttttaatt gtttatttac cggctgttgc agtgaatcct
     3601 gaaaacttgc ctcactaagc cgctgaagtt ttccgttcgc ataaagcact acgcggtaat
     3661 tgtcaaaatt atccttataa tcgcaggcaa taatcttgtc ctgattctct tcgcggttgg
     3721 cgtaggagat gtctatcggg gcgtaaatga aacctatcag cctgtcggca ttagccggac
     3781 ttttcagttt gtccgcggtt atgagttttt ccttgaccag cacatccagg ctggctggcg
     3841 gacggtctac gcctctctgg gtaagggcga aaaaaatatc ctgcaaattg tgcatcgtct
     3901 gattgacaag cacggactcc ctggcgtttt tctgaaccgt gttgtaagct atcattaacg
     3961 tcggcgtgcc tatgactgcc gccaggacaa aagtcgacgc aagcgggtac agacaaatat
     4021 gaaaaatttc gccagccgac aattttttct gccacgtcca catcagaaga taaaacatgc
     4081 cggcaaaagt taccgccgtg cctataatgc cgccgtaaga tatgctggat gcggacgcca
     4141 ccatacccac gcgatagatt ataatcccga aaaaggctgc tatcgaaccg atgaggaaca
     4201 tggctagaat tcccatgccc agcggcattt ttttgacaat tccggttttc tgtatgccgg
     4261 tggagatgaa ataccctcct atcatcaggc tgacgaaact gatgataacc catatcggta
     4321 tttcccacgg caggaaaaaa tagtccgcgg cagttgctaa cacgtaaatg ctcctgtcca
     4381 gtataaacag tataaaaata tgaaaagcat cgcgacacgg ctggcccgat gcgcacaata
     4441 aaagccgaat cgctcatata atacgtcttt ttgtcgccac tgcaagcctt atgaaccatt
     4501 aatttggcta acgtcgctca ttcgacatgt ttaatgatgg aaaaatcgcc cgtctgagcc
     4561 gctttcaaaa ccgccccaag atattcatcc accatatcag cggacatcgg caatggcata
     4621 ttttgtagtt tgctggctaa agcgggcttt ttcgcagcgg aaagtatgcg ttttatgtgt
     4681 aattcaccga atttcggcac atcgctgaat tttgtcacaa aaccgagttt tttactctgg
     4741 cacaccattg cttcggcaac ggcaagcccc agttcgacgc cggcgagaac atcggggttt
     4801 tccttcaaaa agcctgccct gcgatagatt tccgccaccg gcctcagtcg gtcctctatg
     4861 accggcgcaa agaaaaccac aaaatacggc tcgagcaatg cgcacgcccg gccgtgcggc
     4921 atgacatcga ccagtgaaaa actcgacaga tgcgccccgt ttgtgccgcc cgtcattatg
     4981 gatatcgctc cgcagtcagt gcccaggccc agcatttcgc gcaggtggct gtcagacgga
     5041 ttttccgtca atttttcgat gtttgaaact atcaggtcaa tgccggccag cgccacgtca
     5101 cgaactttgg ctgcgtcggg tatgcccatc catacctcca ggcagtgcgc caagccgtcg
     5161 aacgcaccgt ccagcgtaaa atcgcgcggc atggtcacgg taagattgta atcgaaaatc
     5221 gcgcgagtcg gcgttatggc gtcgtcgatt atgagcattt tctggccgac cgtcaaatcc
     5281 gtaatgttcg aatatttggt aagatgcgag ccgctcgcgc tagccagttc cgcagctgca
     5341 aacggccgga gttttctgcc cgttttttca atcaattcgg agattttccc aacgccgaag
     5401 tagtcgtaca ttccgcacat gtcaccgaat acggcatgga cgttagccgc tttggcggca
     5461 tcgagattgc ttccgccgcc gacggctacg acgaagtccg ggtcacgtct gcggatttcg
     5521 tccgtaattc gcatcacgtc ttcgaagggc gaattttccg ccgcagccgg tatcagttcg
     5581 ccgagtattt ccactccagc cgcctcaaac gaggtgcgaa gccgcgcgtg aagtcgactg
     5641 ccccactccc tgccgaagcc gctaactatg accgccgcac gccggcccag cacggcaaaa
     5701 tgttcgccga ttctgtcaaa acagccgcag ccgaaaatgt aatcctcgcc gcagaattcc
     5761 gaaattttac gcctggccgt atctgccgcc gttttcatga gcttttacct cgcatcgcca
     5821 aagatttgca ggtcacgcac aacagataca cactgccggc gattattatt atggtagcac
     5881 ccgtcggcag ctcaggctga tagctggccg caaagcctgc aatgatgaat ccggcacata
     5941 tggccgtcgc ggctagcatc atcgaccaca gccgccgcgt aaacatcctg gcgatggcag
     6001 ccggcagcgt cagcaacgct ataaccatga cgattccaac caccgtcgaa agcagcacca
     6061 ccgtaagaga cgtaactatc agcagcagca gaaaccacca gtgcactttc atgccgcggg
     6121 cacgcgcgaa ctgttcatcg aaacataccg ccagcagttg acgataaaac gtcagcccga
     6181 caccaagtac cagcacgtcc agaatgcaga taagatagat gtctttggcg gttattaaaa
     6241 gaatattgcc gaacagatag ctcatcaaat cctggctgta gcccggcgtc gccgatacga
     6301 acatgacgcc gacggccatt ccgatagccc acatgccgcc gataatcgta tcttcgtgcg
     6361 cacgcgcatg cagcgaaacc agtcctataa ttatcgcagc agccaccgca gcagccacgg
     6421 cgccataaat gggatccagc cacgcccatc cgcagacgtt gttcagataa tacgccgcgc
     6481 ctatgccgcc gagaacgcaa tgcgctatgc cgcccgcgat atacgaaatt ctgcgcacga
     6541 ccacatagct gcccaccacg ccgcaggcta tgctgaccat cagcccgccg agcagggcta
     6601 tctgcaaaaa tggttcattc acaagtgcgt tgaaaaaaat attcatcgtt cgtgacttcc
     6661 ttcgcaacct ttctcgacgc agttatgttc gtgacgcacc cgccgcaccg gcccgccgta
     6721 taattcgtcc atcatccgtt cgtcccattt ttccgtcgga tgcaccgcca ccctgccgtg
     6781 gacgcataat acagttttta cgtattcgct cacgaaagcc atgtcgtgcg aaacggttat
     6841 tattgtcatg tctttattta gccgccccag caattcataa aactcgctgc cgacctgcac
     6901 gtcaaggctg gcggcgggtt cgtccataag cagcatttcg ggtttcgggg cgattgcgcg
     6961 agcgatgagc acccgttgtc gctggccgcc ggaaagagcc gaaaaattcc tgcccgcaaa
     7021 tttgctcatg cccactgccg ccagagcctc tatcgcggca tttcggtcct ctccgccgaa
     7081 tactccgaaa aaaccggccc ggtcggcaca gcccatgcgc accacgtcca gaacgctcac
     7141 cggaaaattc atgtccagcg cgatgcgttg cgccagataa cctattttgc cgcgtgcttc
     7201 ataaggcggc agacccagta tgcggatatc gccacgcgtc ggctcaagca ggccaagcaa
     7261 caacagtacc agagtagttt tgccgccgcc gttaggcccg actatcgaaa cgaaatcccc
     7321 gctttcgata tggaaattca catcctgcaa cacaagttgc gaagaatcgt agccgaagtc
     7381 cagattttta acttcaatcg caatggccat tgagcggttt ttctcctccg agtgcggcgg
     7441 caatttcatc tgccacttta ttgaggtttt taaggtaatc tgccgaaagg ctgtccagcg
     7501 gtattactgc gccgccgatg gcattggcga tgctttgagc cgcatgcctg gaaaactgcg
     7561 gctgaacgaa aattattctg acgttctgcc ggcgtgcatt gtcgataagc tgctgcagct
     7621 gagccgccgt cggcgatttg ccctcgactt caaccgcctc ctgcgtcaag ccgaattcgt
     7681 gtgcaaaata gccgaatgtc ggatgaaaaa caaaaaacat ccggccttta tatggggcaa
     7741 gaagtttggc gatgcgatgg ctgacggcag taaggtcggc aactaaccgg ctacaattcg
     7801 tttcatacaa ttccgcatca gcagggtcga gtttcacgag ttcgtctcga actgtcaacg
     7861 cctgcgttat catgagttcc ggactcatcc agatatgcgg gtcgagcatg ccgtgttcat
     7921 ggccttcgtg gcgttcgtgt tcttcatcat cccgactcat cggcagaagc gttatgccgc
     7981 gacgaaggtc tattatcggc aatttgggaa aaagttccct tattctgggg ataagtgcct
     8041 cttcgaaagg cacgccggta cggaaaaata cagctgcgcg gccaagctga gatatctgtc
     8101 tgccggtggg ttcgtaatcg tgaggttctc tgccagccgg cacaaggaca gatacgctta
     8161 cgcggctgcc gccgatgtgc tcgaccagaa atctgtccgg ttctatgctg acgaagacgt
     8221 ccaatttccc attgttttgt gaggtcgtgg aattcttaca gccgccggct aacaggcaaa
     8281 acaatatcgc aatcacactt gactttttca tgattttcct ttgtgccggt tcgggcggaa
     8341 aaacgtgact ttcatatccc gtaatgatat aattttgcca cggcgttggc aagtccgtta
     8401 tatttgacgt caatgcactc aaggagattt tttattatgg aaaatcagca gatgcccccg
     8461 ctaccgtccg tatcgcagac agtcccgtcc gccaca
//
LOCUS       DAONFI010000066         8342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9868_length_8342_cov_4.261011, whole genome shotgun sequence.
ACCESSION   DAONFI010000066 DAONFI010000000
VERSION     DAONFI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9868_length_8342_cov_4.261011"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PLK08_03305"
     CDS             complement(<1..1103)
                     /locus_tag="PLK08_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPS52559.1"
                     /translation="MRIALDARTIFAANRRGTGKNLIDLYLYAAKARPSWEFVMIHRD
                     RHEPNPFEGIANIRQKTGEIIGDRWNVWLHVRLPLLARAINADVLHCPANVSPYLPMI
                     PMILTVHDIIPMHCPDRRYAKWWRKNVARGIKRARLVFTPSEFSRQQILNEFGDFADK
                     IVVNHWAPDTGCRFIEDAAELTKIREKYALPAGRRFFLSFGARDGRKNTHRIIEAWKN
                     LPAELRNEFSLLIVGLNKPAMQEFSVLLDDYRIRQSCILHGFAPEEDISALLSAADAL
                     CYVSLYEGFGLPILDAFTCRTPVLAGNATSLPEVAGDAAVFVDPQDTDAITHAMRRIM
                     ADASLRESLVDRGLSRVKEFTWNRCVENYVTAV"
     gene            complement(1100..2260)
                     /locus_tag="PLK08_03310"
     CDS             complement(1100..2260)
                     /locus_tag="PLK08_03310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS52560.1"
                     /translation="MKIIVACYTDLRNGGGGATVAKNIAVALKSRGYHVAMLSRADSA
                     DKTGCDEYESIPVEYFYAPSGKFWRVPSIGRSSRIAKVLARHEKPDVFVGVSVYMMNA
                     AQKVWPDVRRVFLFPCLLMHCLKFTNPQPDFWARLDRYSIGFQERKALLNSDRIIFQS
                     PVMLKDAMSLASFDEDKAVICPHGSVHAGRLPRATRSEMREKLSTPQNAFLMLCAGSL
                     DANKNVGWIIESLARLKRDDVWLWIAGDGPQKAELLKKTAKLGLEKRVQFLGQRDDMP
                     ELYGAADLFIHAAWYDALPFVCSEAMSFALPVLGPENHWPKVTSSMEYMINDKRQGWL
                     YDLENPDGLYNAMRIAIENREKLPEMGKSAQKREHIILNWNNYVNAVEGAFR"
     gene            complement(2296..3540)
                     /locus_tag="PLK08_03315"
     CDS             complement(2296..3540)
                     /locus_tag="PLK08_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS52561.1"
                     /translation="MKTLMMICHYFRPDTAVGALRPLRFAQHLKDFGWRAEILTSDKG
                     KTDEKISDDEFDVTRIKPCDENLLGIMTRPLYPESLRKKVQAGLLWRTRRYFSFPDPH
                     TFVNRWYWPLVKEARRLIRSGNIDAVWVTSSPQTYLCIAERLARDAKVPVILDMRDEW
                     TNNFLYEYKGLRNMIARRIEASAIHHAAAVTPITPLAAEKLRQRYPLDAAKITCIQNG
                     FHDEDIPTAQPRNDGRFMVAMMGQSYENHAELFSAIRIARELDADFAHRFIFRWVGYQ
                     GDEKDISGVELFPRVPQCNALKMVAAADAFWLEVPVNGNTDIIICSKTYEYLAMRRPI
                     IGTMPKKSCNADIVKKICNCRFISSRKPAEMAELLLESFNLWKEGRLVAEVSEDALNQ
                     FEATSLTLKLCSILDDITDTGG"
     gene            complement(3537..5108)
                     /locus_tag="PLK08_03320"
     CDS             complement(3537..5108)
                     /locus_tag="PLK08_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52562.1"
                     /translation="MGSGRHLKNLSASWVGYAANFIILMYLSRLVVRSLGEELFGVWS
                     LIVSVTGYMGLADIGLRNGLGRFINFYQGKGETRKVNEVISTSMTFFVFLGIPIFVAS
                     WFIGENFCWLFQRSVPQSLADGCFYAMLLTGANLWLGFLASPYSLAVISQDRFDLFHV
                     TNLVVLGVRAAGTVIVMKNGGGIVQLAMVQVISNLVAVTVFFVIGHRVYPPLYVRPSL
                     ANMLRFKELFGFSFWAFLSAIATKLLYFTDIIVIGATMHSAVFVTLYSIPLTLIDHSR
                     NFVGQVTEVLTPRTIKMSSRNDFASLQLMLKWGSKLTMFFAIPFFTGLMFFGPKFMAL
                     FWGEKFWQSKWVLVLLVVPQYTAMAVRIGGSIISGLGHIKVGALITLLQAGMNLILTL
                     IFILLCGMGIEGVALGTLVPMVIFNVIIAILLHRWVHGSIRRFFTTNVLRWLLSLAGM
                     LIFGYAAKSLAIDSAKYFGAFWDGWLRFGFGLLFYTVGAMLISWFLGFNTHEREILVT
                     ELSSILPWKNKRKPQ"
     gene            complement(5109..6269)
                     /locus_tag="PLK08_03325"
     CDS             complement(5109..6269)
                     /locus_tag="PLK08_03325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS52563.1"
                     /translation="MIENRVIVCFASGYDAPPTSKHHVAHILAEHNVVLWVNYHASRT
                     PSATADDARYILKKLSQVFGGIKEMRKNLFVLTPLVLPFPASKAAKKINRILLGWQIR
                     RAVHQIAAGRPLHVWSFAPDVAYCIDSLHPEKVIYYCVDDFAHFTGYDPNQVRRDEQA
                     LCRRSDLVVATSQNLYDDRIKDNPNTLLVPHGVDYSHFAAAANDDAPLPLPHDVAEIP
                     QPRIGFFGLLRDWVDIKMLAIIASRRTEWQFVLIGDANMNIDEYRKFHNMHFLGRKSY
                     KELPAYCRAFDVGIIPFQMNELTAAANPIKLREYLAAGLPVVSSPMPEVRRYAEKGLV
                     RLASGADEFERAIDAAIKDSPAMRQKYSRAMIDETWPAKIELICEHLMKGGK"
     gene            complement(6266..7450)
                     /locus_tag="PLK08_03330"
     CDS             complement(6266..7450)
                     /locus_tag="PLK08_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HPS52564.1"
                     /translation="MKRLLYIAHRMPYPPDKGERVRAFNEICELARNGSDVTVIAGSA
                     GADDYASAKKLAEYCSAVETFPIVRRSALVRGACSFIMGRSVSEGYFSSCMAGRKIRR
                     LGRFDVAVGYSSSVLNLLMKADAARRIMDIVDADSAKWADYACRTAWPLKKIFNIESS
                     RVARLERKCVEQCNDVVMVSREEAEILGDGIPNLHVAANGVDADFFNPSLLSPITGLG
                     EHAMVFTGSMDYRPNAEAVEWFATEVLPCIREKFDNAIFVIVGRNPSKNVRDMAMMEG
                     VRVTGNVVDVRPYIQAAAVSVCPLHIGRGIQNKVLEAMSMAKAVVVSPQAMSGLDVTQ
                     GQDVELAETSGQWVEKLLYLLSEADVRNMLGYAARQCVLEKYSWHARLKDFVSLCLKD
                     VE"
     gene            complement(7484..>8342)
                     /locus_tag="PLK08_03335"
     CDS             complement(7484..>8342)
                     /locus_tag="PLK08_03335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPS52565.1"
                     /translation="GVLPLFLVKSIFEGKILVSIPYATYGGILADDEDTERLLFEKAR
                     EISVEHGAAYLELRNVSPSHLDLPVIARYDTFIKSLPADPDDVLKGLPKKARAATRNG
                     LKILQPRIGDELMDDVYDLYAYTLRRLGSPNYSRSLWRELKNNYGKNCVTLGVFEGDK
                     MLAGVVSFIFRDTMVAYFSGSLPRGMKLGANNVMYVSLMEYAVKQGLRQFDFNRTRRN
                     NSGPYAFKRHMGFEPSQLHYQIALNRSTTLPNLSPGNSGFSLAIKIWRHMPLFATRIA
                     GGIVTRWIP"
BASE COUNT         1816 a         2229 c         2234 g         2063 t
ORIGIN      
        1 tcgacggcgg taacgtaatt ttctacgcac ctgttccacg tgaactcttt tacccgcgaa
       61 aggcctctgt cgacgagaga ttcccgcagg ctggcatcgg ccataattct tctcatggca
      121 tgtgttatgg cgtcggtatc ctgcgggtcc acgaagacag cggcatcgcc ggcaacttcc
      181 ggcagcgacg tggcattgcc ggccagtacc ggcgtacggc aggtgaatgc gtccagtatc
      241 ggcaaaccga agccttcata cagcgatacg taacaaaggg catctgcggc actgaggagc
      301 gcagatatat cctcctccgg tgcgaaacca tgcagaatgc aactttgccg aatcctgtaa
      361 tcgtcgagca gcacggagaa ttcctgcatg gccggcttgt taaggccgac tatcagcagg
      421 ctgaattcgt ttctcaactc agccggcaga ttcttccatg cctcgatgat tcgatgcgta
      481 ttttttcgtc cgtcgcgtgc accgaacgac aggaaaaaac gtctgccagc cggcagtgcg
      541 tatttttcgc gaattttagt cagttcggcg gcgtcttcga tgaaccggca gccggtgtcc
      601 ggcgcccagt gattcactac aattttatcg gcaaaatcgc cgaattcgtt gagtatctgc
      661 tgcctggaaa attcgctcgg cgtaaaaacc agtcgggccc gcttaatgcc gcgagctacg
      721 tttttccgcc accatttcgc gtatctcctg tcgggacagt gcattggaat gatatcgtgt
      781 acggtcagaa tcatcggaat catcggcaga tacggagaaa cgttagccgg gcagtgcagt
      841 acgtcggcat ttatcgccct ggcgagcagc ggcagacgta cgtgcagcca cacattccag
      901 cgatcgccga tgatttcacc ggttttttgt cgaatattcg caataccttc aaaaggattc
      961 ggctcatgcc tgtcgcgatg aatcatgacg aattcccacg atggccgtgc ctttgccgca
     1021 tacaagtaaa ggtctataag attttttcca gtcccccgac gatttgccgc aaaaatcgtc
     1081 ctggcatcga gggctattct catctaaatg ctccttcgac tgcgtttacg taattgttcc
     1141 aattaagtat aatatgttct cttttttgtg ctgatttgcc catttcgggg agtttttctc
     1201 tgttttcaat ggctatgcgc atagcgttgt atagcccgtc ggggttctca aggtcgtata
     1261 accagccttg tcttttgtcg ttaatcatgt attccattga gcttgtgact ttgggccaat
     1321 gattttcagg gcctaatacc ggcagggcaa aactcattgc ctcgctgcat acgaaaggta
     1381 atgcatcgta ccatgcggcg tgtatgaaca gatccgctgc gccgtacagc tccggcatgt
     1441 cgtctcgttg tcccaggaat tgaactcttt tttcaagtcc gagtttcgct gtttttttta
     1501 gcagttccgc tttttgtggg ccgtcgccgg caatccacag ccatacgtca tcgcgtttca
     1561 gcctggcgag cgactctata atccaaccca catttttgtt ggcatcgaga ctgccggcgc
     1621 agagcatcag gaaagcgttt tgcggggtgg agagtttttc gcgcatttca ctgcgggtag
     1681 cgcggggcag gcgaccggcg tgtacgctgc cgtgaggaca tatgacagcc ttgtcttcgt
     1741 cgaaagaggc cagcgacatt gcgtccttca gcattactgg actttgaaaa atgattctgt
     1801 cgctgttcag gagagctttt ctttcctgaa aacctatcga atatctgtcc agccgtgccc
     1861 agaaatccgg ctgcggattc gtgaatttaa gacagtgcat cagcaggcac ggaaacagga
     1921 atacccgcct tacgtcgggc cagacttttt gggcggcatt catcatataa acggatacgc
     1981 cgacgaatac gtccggtttt tcatgccggg ccagcacttt ggctatgcgc gagcttcggc
     2041 cgattgacgg tacgcgccag aattttcccg atggcgcata aaaatattcc acgggtatgg
     2101 attcgtattc atcgcagccg gttttatccg cactgtctgc gcgcgacagc atggccacgt
     2161 ggtagccgcg ggatttcaat gcgactgcga tatttttagc tacggtcgct ccgcctcccc
     2221 cgttgcgaag atcggtataa catgccacta taatcttcat caacgtcctc tgctagaaga
     2281 tacagtctct tcgggtcaac ctccggtatc tgttatatcg tcaagaatgc tgcacaactt
     2341 taacgtcagc gatgttgcct cgaactggtt cagggcatct tcgctgacct ccgcgacaag
     2401 ccggccttcc ttccataaat tgaatgattc gagcagcagt tccgccattt ccgccggctt
     2461 gcgagacgaa atgaatctgc aattacagat ttttttgacg atatccgcat tgcagctttt
     2521 tttgggcatt gtgccgatga tgggccgccg catggcgaga tattcgtaag ttttggaaca
     2581 tatgataatg tcggtgtttc cgttgaccgg cacttccagc cagaatgcgt cggcggctgc
     2641 gaccattttt aatgcgttgc attgcggaac tcgcgggaaa agttccacgc ctgaaatgtc
     2701 tttttcgtcg ccttgatagc cgacccagcg gaagatgaag cgatgcgcaa aatcggcgtc
     2761 aagttcgcgg gctattcgta ttgcactgaa aagttcggcg tggttttcat aactctgacc
     2821 catcatggcg accatgaatc tgccgtcgtt gcgtggctga gccgtcggga tatcttcgtc
     2881 gtgaaaaccg ttctgtatgc aggttatttt tgccgcgtcc agtggatatc gctgtcggag
     2941 tttttctgcc gccagcggcg ttatgggcgt aacggcggcg gcatggtgaa tcgccgacgc
     3001 ttcgattctg cgggcaatca tattccgcag ccccttgtat tcgtataaaa aattgttcgt
     3061 ccactcatcg cgcatatcca ggattacggg tactttggca tctctggcga gtctttcggc
     3121 gatgcagagg taagtctgcg gcgacgaggt gacccacacg gcgtcgatgt tgccggagcg
     3181 gatgagtcgt cgggcttctt ttacgagcgg ccaataccat cggtttacga aagtgtgcgg
     3241 gtcagggaaa gaaaaatatc gtcgcgtccg ccacagcagg ccggcctgca ccttctttct
     3301 cagcgattcc ggatagagcg gtcgcgtcat tatgccgagt aaattttcgt cgcagggttt
     3361 tatgcgggtc acgtcaaact cgtcgtcaga gattttttcg tcggtttttc ccttgtctga
     3421 cgtgagaatc tcggctcgcc agccgaaatc tttgagatgt tgggcgaaac gcaatggccg
     3481 cagggcgccg acggcggtgt cgggtcggaa atagtggcat atcatcatca gcgtcttcat
     3541 tgcggtttcc ttttgttttt ccacggcagt atggagctga gttccgtgac gagtatttcc
     3601 cgctcatgcg tattgaatcc gagaaaccac gaaatcagca ttgcgccgac ggtatagaac
     3661 agtaggccga acccgaatct cagccagccg tcccagaatg cgccgaaata ttttgcactg
     3721 tcgatagcca ggcttttcgc cgcgtaaccg aatatcagca tgccagccag cgagagcagc
     3781 caccgtagaa cattcgtcgt gaaaaatctg cgaatcgacc catgcaccca cctgtgcagg
     3841 agaatcgcta tgattacgtt gaatatgacc atcggcacaa gcgttcccaa cgccacgcct
     3901 tctattccca taccgcacag gagaatgaaa atcagcgtga gtatgaggtt catgccagcc
     3961 tgcagcagcg ttatgagtgc accgactttg atatggccca gcccgcttat gatgctgccg
     4021 ccgattcgta cggccatggc ggtatattgc ggcactacca gcagcactag cacccacttg
     4081 ctctgccaga atttctcgcc ccagaacagg gccatgaatt tgggcccgaa aaacatcagg
     4141 ccggtaaaga acggtatcgc aaaaaacatc gtaagtttcg agccccattt cagcatcagt
     4201 tgaagcgagg cgaaatcatt gcggctggac atcttgatgg tgcgaggcgt gagcacttcg
     4261 gtaacctggc cgacgaaatt gcggctgtgg tcaataagcg tcagcggtat gctgtagagc
     4321 gtgacgaata ccgccgagtg catagttgcg cctattacga tgatgtcggt gaaatatagc
     4381 agcttcgtgg cgatagccga cagaaacgcc cagaaactga agccgaaaag ttctttgaat
     4441 cgcaacatgt tcgccagcga cggccgtacg taaagcggcg gatagaccct gtggccgatg
     4501 acgaaaaaca ccgtcactgc gacgagattg gaaatcacct gcaccatggc cagctgtacg
     4561 atgccgccgc cgtttttcat gactatcaca gtgcccgccg ccctaactcc gagcacgacc
     4621 aggttcgtga cgtggaacag gtcgaagcgg tcctggctga tgaccgccag cgaatacgga
     4681 gacgccagaa atcccagcca cagatttgcg cccgtcagga gcattgcata gaaacagccg
     4741 tcggcaaggc tttgcggcac gctgcgttga aacagccagc agaaattttc gcctatgaac
     4801 catgatgcca cgaatatcgg aatgcccaga aacacaaaaa aagtcatcga cgtgcttatc
     4861 acttcgttga cttttcgcgt ctcgcccttg ccctggtaaa aatttatgaa tcggccgagg
     4921 ccgttacgca ggcctatgtc cgccagaccc atgtaacccg tgacggaaac aatcagcgac
     4981 catacgccga aaagttcctc gccgagtgac cgaaccacca gccgcgaaag gtacatcaat
     5041 atgatgaaat tcgcggcgta gccgacccag cttgcggaaa gatttttgag atgccgtcct
     5101 gaacccattt actttccgcc tttcatgaga tgttcgcaga tgagttcgat ttttgccggc
     5161 catgtttcgt caatcatcgc ccgtgaatat ttttggcgca tcgccggcga atcttttatc
     5221 gcggcgtcga tagcccgttc aaattcatcc gcgccgctcg cgagccgcac aaggcctttt
     5281 tctgcgtatc gtctgacttc gggcatgggc gacgaaacca ccggcaggcc ggctgcgagg
     5341 tattcgcgaa gttttatagg attggcggct gcggtgagct cgttcatttg gaacggtatt
     5401 atgcccacgt cgaaagcgcg gcagtatgca ggcagctctt tatagctttt tctgccgagg
     5461 aaatgcatgt tgtgaaattt tcgatattcg tcaatattca tattggcgtc gccaatcagc
     5521 acaaactgcc actcagtgcg gcggctggcg ataatcgcca gcattttaat atcgacccag
     5581 tcccgcagaa ggccgaaaaa ccctattcgc ggctgcggta tctctgcaac gtcgtgcggc
     5641 agcggcagtg gggcgtcgtc gtttgccgcc gcggcgaaat gactgtaatc gacgccgtgt
     5701 ggaacaagca gcgtattggg attgtctttt atcctgtcgt cgtaaagatt ctgcgatgtg
     5761 gcgacgacaa ggtcgctacg gcggcataat gcctgttcgt cgcgtctgac ctggttcggg
     5821 tcgtagccgg taaaatgcgc aaaatcatcg acgcaataat agatgacctt ttccggatgg
     5881 agcgagtcga tgcaatatgc cacatccggc gcgaaagacc acacatggag cggcctgccg
     5941 gctgcgattt gatgtacggc gcggcgtatc tgccatccga gcagtatccg gttgattttt
     6001 tttgcagcct tgctggctgg aaacggcaga accagcggcg tcagaacgaa aagatttttt
     6061 cgcatttcct tgatgccgcc gaaaacctgc gaaagttttt tgaggatgta gcgtgcatcg
     6121 tcagccgtcg ccgacggcgt gcgggaagcg tgatagttta cccatagtac cacgttgtgt
     6181 tcagccagga tatgcgccac gtgatgtttg ctcgtcggcg gggcatcgta accgctggcg
     6241 aaacagacta tgactcggtt ttctatcatt cgacatcctt gaggcagagc gagacaaaat
     6301 ccttcagcct cgcatgccac gaatacttct ccagaacgca ctgccgggcg gcatagccca
     6361 gcatgtttcg aacgtctgcc tccgaaagta aatataacaa tttttcgacc cattgaccag
     6421 acgtttctgc aagttccacg tcttggccct gcgtcacgtc caggccgctc atggcttgcg
     6481 ggctcaccac caccgcctta gccatggaca ttgcttcaag caccttgttc tgtatgccac
     6541 ggccgatatg cagcggacag acggatactg ctgccgcctg aatgtacggc cttacgtcta
     6601 cgacgttgcc tgtgacccgc acgccttcca tcatcgccat gtcccgcacg ttttttgagg
     6661 gatttcggcc gacgatgacg aaaatggcgt tgtcgaattt ttcgcggatg cagggcagga
     6721 cttcggttgc gaaccattcg acggcttcgg cgttgggccg gtagtccatc gagccggtga
     6781 agaccattgc gtgttcgccc agtcccgtaa tcggcgacag aagcgacggg ttgaaaaaat
     6841 ctgcgtccac gccgttggct gccacgtgca gattgggaat gccgtcgcct agaatctcag
     6901 cctcttcccg cgataccatc accacgtcgt tacattgctc tacgcatttt ctttccagcc
     6961 gcgcgactct ggatgattcg atattgaaaa tctttttcag cggccaggct gtcctgcatg
     7021 cgtagtcggc ccacttggcc gagtcggcat ccacaatgtc catgatgcgt ctggcggcgt
     7081 cggctttcat tagcaggttc aataccgacg atgaatatcc cacggcgacg tcgaaccggc
     7141 cgagccgtct tattttccgg cccgccatgc aggatgaaaa atatccttcc gatacgctcc
     7201 tgcccatgat gaaggaacac gcgccgcgga cgagtgcgct gcgacggact atggggaagg
     7261 tttcaacggc tgaacagtat tcagcgagtt tttttgcgct ggcgtaatcg tcggctcctg
     7321 cgctgccggc gattacggtt acgtcagaac cgtttcgggc cagctcgcat atttcgttga
     7381 acgcacgcac gcgttcgccc ttgtcgggcg ggtacggcat tcgatgcgct atgtacagca
     7441 gtctcttcat ggttcgttac cgtcttgtcg gttggcggcc tgtttatggt atccatctgg
     7501 tgacgatgcc gccggctatt cgcgtggcga agagcggcat gtgtcgccag attttgatgg
     7561 ctaatgaaaa accgctgttg cccggactca gattgggcag tgtcgtcgag cggttcaacg
     7621 ctatctggta atgcagttgc gatggctcga aacccatatg tcgtttgaat gcgtaagggc
     7681 cggagttgtt tcggcgggta cggttgaagt cgaactgtcg caggccctgt ttgacggcgt
     7741 attccataag cgaaacgtac attacgttgt ttgctccgag tttcatgccg cgcggcagtg
     7801 agccggagaa gtaggctacc atcgtgtcgc ggaatatgaa cgacacgacg ccggctagca
     7861 ttttatcgcc ttcgaaaacg cccagcgtca cgcagttttt gccgtaattg tttttcaact
     7921 cacgccagag agacctgctg taattcggcg aacccagccg tcgcagcgta taagcgtaca
     7981 agtcgtatac gtcgtccatg agttcgtcgc cgatgcgcgg ctgaaggatt ttgaggccgt
     8041 tgcgtgtcgc cgcccgcgcc tttttgggca gacctttgag tacgtcgtca gggtcagccg
     8101 gcagcgattt tataaacgta tcgtagcggg ctattaccgg taaatcgaga tgcgagggcg
     8161 atacgttgcg aagttcgaga tatgccgcac cgtgttcgac gctgatttcg cgggcttttt
     8221 cgaagagcag cctttccgtg tcttcgtcgt cggctaaaat gccgccgtat gtcgcatatg
     8281 gaattgagac gagaattttg ccttcaaaaa tgctttttac taaaaacagc ggaagaacgc
     8341 cg
//
LOCUS       DAONFI010000067         8324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9897_length_8324_cov_4.527633, whole genome shotgun sequence.
ACCESSION   DAONFI010000067 DAONFI010000000
VERSION     DAONFI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9897_length_8324_cov_4.527633"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="PLK08_03340"
     CDS             <1..1557
                     /locus_tag="PLK08_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HPS52566.1"
                     /translation="QNPPELTRNWSCGQEAAFRMMAILFGTVCTLDSQAARPERLNRV
                     AMLAWQTARHIAVNINYARSQGNNHAISEALWLITAGVVFGENFAPASRWLSDGLDIM
                     CREIRRQVMDDGSYAQHSMNYHRLVMDDLPWTFGILRAGGIVVPDVMREKFEKLTCWI
                     EEMADENTGRVPNYGPNDGAKVLPLSCCDYVDYRPTIQAAWYEVFGKSRYHAGCWNEK
                     KLWLYGEESLDAPKSDVKKTAAFNAASGGYYVMRGRTSMAFTRCHTYTRRPNQNDMLH
                     VDLWHEGKNILRDAGSFSYNAPEPWSGYFSSTAAHNTVTVNGENQMVRGPRHLWFRWT
                     KSRLLEWFVSDDGSIGFFSGEHYGYSRFGATHRRSILRIGDGYVILDEIIGRRKEMKT
                     ELNWRLISDDWQAGFTGGGLRVVCPEATITVFAETGFDVSFDESGDAGPSGWESLYYG
                     QKSPCPYIRVAGSSKAGTTKFVTIVSTGSTEMFPAMLDAMKTDAAVAMRHITEKFVDD
                     VRHLTGMEMN"
     gene            1558..2787
                     /locus_tag="PLK08_03345"
     CDS             1558..2787
                     /locus_tag="PLK08_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018764982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS52567.1"
                     /translation="MKILIVHQYFLGKDDAGGSRWNQFAKYWAQAGHEVTILAGMVHY
                     ASGKKSEQYKGKFIVTEDIMPGVRCIRCHVSESYNRSFMGRAWAYMSFTFSSLVAGFF
                     KTHKPDIVIATSPPLTVEPTMKILAAAYRVPCVFEVRDLWPESAIDTGVLTSKPLIKM
                     AYYMESQAYKAARWINVLTPAFVDALVEKKNVPREKISMIPNGADLDIMRPEPKDAGL
                     QSELGIDGRFVISYFGAHGRANKVGQLLDVAERIKNSMPDVLFMLVGDGMEKAMLKED
                     AAKRNLDNVIFVNSVGKEKVKDYINVSDICTAVLMKNDTFKTVYPNKVFDYMSCAKPI
                     IIGIDGVARKLVEDAKAGLFAEPENPDAFIDAIRKIKDNSELRDAMGKSGLEYVRQNY
                     DRKVLAEKYIDILQKLV"
     gene            2803..3756
                     /locus_tag="PLK08_03350"
     CDS             2803..3756
                     /locus_tag="PLK08_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52568.1"
                     /translation="MAQNASEIEFPELKLSGASVSQHCEALREIGKLPFWWYIGASGI
                     FGNYSLPFSDSNGNWWYQVKPGLCWPADCFAAIDADSACPQLTKTFLGYQHVVAGEPQ
                     ADSHLVINAILDLSAYGTESINAKRRNAIRKGFRSCILTVETGPDDETLDGCRLAWND
                     LTRRTGWKHAQNPDEFNESWRLLAKCPGTSIIVARDSENGQVAGFLVTKIIGDTAYVD
                     TIASRTDMLSVNPNDAVMYAFLMNAKNLPGVTKAHYAIKSNVTMLEKFKTGLGFVPNP
                     FPARTVFRPGVGLALKLLKPKHYNRMMGIFENENRNEAENE"
     gene            3749..4852
                     /gene="wecB"
                     /locus_tag="PLK08_03355"
     CDS             3749..4852
                     /gene="wecB"
                     /locus_tag="PLK08_03355"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPS52569.1"
                     /translation="MNSIKIIVVAGARPNFMKIAPIMESFDASDRIEPVLVHTGQHYD
                     EKMSDLFFRQLGIPSPDVNLGVGSGSHAVQTAEIMKSFEPVLISQQPAAVLVVGDVNS
                     TIACGYVAAKMGIKLIHVEAGLRSFDRAMPEEINRVLTDAISDILFCTEKSGVENLKK
                     EGVTASRIFMVGNVMIDTLLKNLEKAKQSPIMQQLNLKPGSYATMTMHRPSNVDNPDV
                     LVGLLDAIEVIQRDIPIIFPIHPRTRNNIVKFKFADRMKNMPNLRFIEPLGYLDFLLL
                     NANAACVLTDSGGLQEETTVLGTPCITMRENTERPITCEVGTNQIVGTDTDAIIAAWT
                     RVQNGEYDKRGIPEMWDGKAADRITQILLDILG"
     gene            5182..5592
                     /locus_tag="PLK08_03360"
     CDS             5182..5592
                     /locus_tag="PLK08_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52570.1"
                     /translation="MHVKYGDCDNAARYLCVENAKNTDADYQCAVYCSCEAALSAKFQ
                     QTYFPGTFKQSYAPGNECVWFTTIAETRDSRLSDYLQPQSSEHQNVYASSNSIFDGFL
                     SMQDGILVLLISLLGLGGYVFERITVSRRRSIYQ"
     gene            complement(5628..7928)
                     /locus_tag="PLK08_03365"
     CDS             complement(5628..7928)
                     /locus_tag="PLK08_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPS52571.1"
                     /translation="MEVLELANYFRKIALEQSINPSQVQSVAELFAEGGTVPFIARYR
                     KERTGGLDEVNIIAIRDRLDQLQELDKRRQAILKSIQEQGKLTGELEAKIQAAETMNI
                     LEDIYLPYKPKRRTRGTMAKDKGLEPLALLLLEQNPATDPLAEAQKYISTEKDREVPD
                     VAAALAGARDIIAENISENAAARAELRKLYEKNSVMSSKVLKGKEEDAIKYSDYFEWA
                     EPAAGAPSHRILAIRRGAKEGFLTFHIQPDEQQAIDILFRMFVKGNSAAAMQVQAAAE
                     DSFKRLLSLSMETEMRLEMKNRAEEEAIRVFADNIRELLLASPLGQQSVLALDPGFRT
                     GCKLVCLDPQGKLLFDDVIYLHKEDEAEMKIRVLAEKFRIQAIAIGNGTAGRETKAFC
                     DKIDFGRPLPVVMVNESGASVYSASEVARDEFPDKDVTVRGSVSIGRRLMDPLAELVK
                     IDPKSIGVGQYQHDVDQKKLKNSLDDVVTSCVNSVGVEINTASKQLLSHVAGLSEKIA
                     AAIVETRNENGPFTDRREILKVAGMGPKTFEQAAGFLRIRSGNNPLDASAVHPESYPI
                     VERIAADLGCTVGDLMANDELRKKIDLKKYVTDSIGLPTLNDILQELAKPGRDPREPF
                     QLFSFADGIKEMTDLKIGQKLPGVVTNVTNFGAFVDIGVHQDGLVHISELADRFVEKP
                     SDVVKVHQKVEVTVTSIDIDRKRIGLSMRSKPDTSGEGRTQRTSPAAKHHDNTNPQER
                     RDNKKPSRHENDSNNPFKDFFKNYKI"
     gene            8069..>8324
                     /locus_tag="PLK08_03370"
     CDS             8069..>8324
                     /locus_tag="PLK08_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPS52572.1"
                     /translation="MVFDFFDKKGLCVLVGAGVGGREMLTADGEYWLGRADVVIYDRL
                     ADDSLLELVRSDAEMIYVGKQAGDHTMPQEQINALLVKYTL"
BASE COUNT         1943 a         2048 c         2254 g         2079 t
ORIGIN      
        1 cagaatccgc ctgagcttac aagaaactgg tcgtgcgggc aggaggctgc gttccgcatg
       61 atggcgatat tattcggcac ggtctgcacg ttggattccc aggctgccag gccggaacgt
      121 cttaatcgtg tcgccatgct tgcgtggcag accgccaggc atattgcagt caacatcaac
      181 tatgcccgca gtcagggcaa caatcacgcg atttccgagg ctctctggct gataacggca
      241 ggcgttgtat ttggcgaaaa tttcgcgccg gccagtcggt ggctgagcga cggattggat
      301 atcatgtgcc gggaaatccg gcgtcaggtt atggatgacg gttcttatgc tcagcattcg
      361 atgaactatc accgattggt gatggacgac ttgccctgga cattcggaat tctaagagca
      421 ggcggcatag tcgtgccgga tgtcatgcgt gaaaagtttg aaaaactcac ctgctggatt
      481 gaagagatgg ccgatgaaaa taccggcaga gttccgaatt acgggcctaa cgacggagcg
      541 aaagtgctgc cgctgagctg ttgcgattat gtggattatc ggccgactat tcaggctgcg
      601 tggtatgagg ttttcggcaa aagccgttat cacgccggct gttggaatga gaaaaaactc
      661 tggctgtacg gcgaggaatc gttggatgct ccgaagagcg acgtgaaaaa aactgcggcg
      721 ttcaacgctg cctccggcgg ttattacgtc atgcggggcc gaacgagcat ggcgtttaca
      781 cgctgtcata cgtatacgcg caggcccaat cagaacgaca tgctgcatgt cgacctttgg
      841 catgaaggca aaaacatcct gcgtgacgca ggcagtttca gctacaacgc gccagagccg
      901 tggagcggat atttttcatc gacggctgcc cataatactg tcaccgtaaa cggcgaaaat
      961 cagatggtca gagggccgcg gcatttgtgg tttcgctgga cgaaatcaag gctgttggaa
     1021 tggtttgtgt cggacgacgg cagtatagga tttttcagcg gcgaacatta cggttacagc
     1081 cgtttcggag cgactcatcg gcgcagtata ttgcggattg gcgacggtta tgttattctg
     1141 gacgaaatta tcggccgccg gaaagaaatg aagacggaac ttaactggag gctgatttcc
     1201 gacgactggc aggcaggctt tacgggcggt ggccttcggg tcgtctgccc cgaagcaacg
     1261 ataacggttt tcgcggaaac gggttttgac gtctcttttg acgaatcggg agatgccggc
     1321 ccatcaggtt gggagtcgct gtattacggc cagaaatcgc cgtgcccgta tattcgcgtt
     1381 gccggcagtt cgaaagccgg cacgacaaaa ttcgttacga tagtgtccac cggcagtacg
     1441 gaaatgttcc cggcgatgct cgacgccatg aaaaccgatg cagccgtagc catgcgtcat
     1501 atcacggaaa aattcgtgga tgacgtgcgg catttgacag gcatggagat gaattagatg
     1561 aaaattttaa tagtacatca atattttctc ggcaaggacg acgccggcgg cagccgctgg
     1621 aatcagtttg ccaaatattg ggcccaagcc ggccatgaag tgacgatact ggccggcatg
     1681 gtgcattatg cgtctggcaa aaaatccgaa caatacaaag gtaaattcat cgtaaccgaa
     1741 gacatcatgc ccggcgtacg ctgcatacgc tgccatgtga gcgagtcgta caacaggagt
     1801 ttcatgggca gggcgtgggc gtatatgagt ttcacattct cttcgcttgt agcaggcttt
     1861 tttaaaacac acaaacccga tatcgtcatc gccaccagtc cgccgctgac ggtcgagccg
     1921 acgatgaaaa ttctggcggc ggcttaccgt gtgccgtgcg tttttgaagt ccgcgacctt
     1981 tggccggaaa gtgctatcga caccggcgtg cttacgtcaa agccgctgat aaaaatggcg
     2041 tattacatgg aatcacaggc gtataaggcg gccaggtgga taaacgtcct tacgccggcg
     2101 ttcgtcgatg cacttgtcga aaagaaaaac gttccccgcg aaaaaataag catgataccc
     2161 aatggcgccg atttggacat catgcggcct gaacccaaag acgccggctt gcagagtgaa
     2221 ttgggaatag acggccggtt cgtcataagc tatttcggcg cgcacggccg agccaacaag
     2281 gtcggccagc tgctggatgt ggctgaacgg ataaaaaatt ccatgccgga tgtccttttc
     2341 atgctcgtcg gcgacggcat ggaaaaggct atgctgaagg aagacgctgc aaaaagaaat
     2401 ctcgacaacg tcatatttgt gaactcggtc ggcaaagaga aagtcaagga ttacataaac
     2461 gtctctgaca tctgcacagc ggtcttgatg aaaaacgaca cgttcaaaac cgtttatcca
     2521 aacaaggtgt tcgattacat gagctgtgcc aagccgataa taatcggtat cgacggcgtt
     2581 gccagaaaac tggtcgagga cgcgaaagcc ggcctctttg ccgagccgga aaatcccgac
     2641 gccttcatag acgcgatacg gaaaataaaa gacaattccg aattgcggga cgcgatgggc
     2701 aaatcgggcc tggagtatgt tcggcagaac tatgaccgca aggtgcttgc cgagaaatat
     2761 atcgacatcc ttcagaaatt ggtatgaaag aatgaggcga cgatggctca aaatgcgagc
     2821 gaaatagagt tccctgaatt gaaactatcc ggcgcaagcg tgtcgcagca ttgcgaagcg
     2881 ttgcgtgaaa tcgggaaact gccgttctgg tggtatatcg gagcgtcggg gatttttggg
     2941 aattattcgc tgccgttcag cgattcaaac ggcaactggt ggtatcaggt caagcccggc
     3001 ctctgttggc cggctgactg ttttgccgcg atagacgccg attcggcctg tccgcaactg
     3061 acgaaaacat ttttgggtta tcagcacgtt gttgccggtg agccgcaggc tgacagccac
     3121 ctggtaatca atgccatact cgacctttcc gcatatggta cggaatccat taacgccaaa
     3181 cggcgcaatg ccatacgcaa aggctttcgt tcctgcattt tgacggtgga aaccggtcct
     3241 gacgacgaaa ctctcgacgg ctgccgtctc gcctggaatg acctgacgcg gcgtaccggc
     3301 tggaaacatg cacaaaatcc cgacgaattc aatgaatcgt ggcggctgct ggcgaaatgc
     3361 cccggcacaa gcataatcgt ggcacgcgac agcgaaaacg gacaggtcgc cggttttctg
     3421 gtaacgaaaa taatcggcga caccgcatat gtggacacaa tcgcgtcgcg gacggatatg
     3481 ctgtccgtaa atccgaacga tgccgtcatg tacgcatttc tgatgaacgc aaaaaatctg
     3541 ccaggtgtaa cgaaagccca ctatgccata aaatccaatg ttaccatgtt ggaaaaattc
     3601 aagaccggct tgggttttgt gccgaatccg tttccagcca gaacggtttt tcggcctggc
     3661 gttggtttgg cattaaaact tctgaaaccg aagcattaca atcgtatgat gggcattttt
     3721 gaaaatgaaa acaggaacga ggcggaaaat gaatagcata aaaattatcg tagttgccgg
     3781 cgccaggccg aatttcatga aaattgcccc gattatggaa agcttcgatg cgtctgaccg
     3841 tattgaaccg gtactcgttc ataccggcca gcattacgat gaaaaaatga gcgacctgtt
     3901 tttccgccag ctcggcattc cttcgccgga tgtgaatctt ggagtcggca gcgggtcgca
     3961 cgcggtacag acggctgaga ttatgaaatc atttgagccc gttctgatat cgcagcagcc
     4021 ggctgctgta ctggtagttg gcgacgtcaa ttccacgatt gcctgcggct acgttgccgc
     4081 caagatgggc ataaaactca tccacgtcga agccggcctt cgcagcttcg accgtgccat
     4141 gccggaggaa atcaatcgcg ttctgaccga cgccatcagc gacattctgt tttgtacgga
     4201 aaagtccggc gtggaaaatc tcaaaaaaga aggcgtaacg gcctctcgga ttttcatggt
     4261 cggcaacgtt atgattgaca cgcttcttaa aaatttggag aaggctaaac aaagcccgat
     4321 tatgcagcag ctcaacctga aaccagggtc ttacgccacc atgaccatgc atcggcccag
     4381 caacgttgac aatcccgacg tgctcgtcgg cctgcttgac gccattgaag tcatccagcg
     4441 tgacatcccg ataattttcc cgattcatcc gcgaacgcgg aataacatcg tcaagttcaa
     4501 gtttgccgac cgaatgaaaa atatgcctaa cctgcggttc attgagccgc tgggttatct
     4561 ggattttctg ctgcttaatg cgaatgccgc ctgcgtgttg accgactccg gcggcttgca
     4621 ggaggagacg accgttttgg ggacgccctg cattaccatg cgggaaaata ccgaacggcc
     4681 aataacgtgt gaagtcggca cgaatcagat tgtcggcacg gatacggatg ccatcattgc
     4741 cgcctggacg cgagtacaga atggggaata tgacaaacgc ggcattccgg aaatgtggga
     4801 cggcaaagcc gccgaccgca taacgcaaat tttgctggac atactgggct gatttacata
     4861 taatcccgcc cgataatttt ggaattcgca tgtgacggta tttttgcaat gaaaatccgc
     4921 ctctgcgaaa gatgctttca aaaagcgaaa ggtattcttc cgttttaatt caaagctatc
     4981 ccccaattat ggccggagca tctcaaattc aagatgttct gtttttgtgg gtagctttta
     5041 gagcgtgctc tagggatgta caggatgaac cgggcgttta ttggaaaaat ccttttttgc
     5101 cacattgtta tcggtgcgtt ttgcgctgat gctgcactat gcgcttccgt aagcgacgct
     5161 gattgtggga atcattcgga tatgcatgta aaatacggcg attgcgataa tgccgcgcga
     5221 tatttatgtg tggagaatgc aaaaaatacc gatgcggatt atcagtgcgc agtatattgc
     5281 agttgcgaag cggctttaag tgccaaattt cagcagacat atttccccgg gacttttaag
     5341 cagtcttatg cccctggcaa tgaatgcgtc tggtttacga cgatagctga aacccgcgac
     5401 agccggttaa gcgattatct tcagccgcaa agttccgaac accaaaacgt atatgcttcc
     5461 agcaacagta tatttgacgg tttcttgtcc atgcaagacg gcattctggt gttgctcatt
     5521 tcacttctcg gcctgggcgg atacgtattc gaacgtatca ccgtcagccg ccgccgttcc
     5581 atttatcagt aatgaattcg tttgcagttt tttccattat gcagccgtca gattttataa
     5641 tttttgaaaa aatccttaaa agggttattt gaatcgtttt catgtcgcga tggtttttta
     5701 ttatcacggc gttcctgtgg gttggtgttg tcgtgatgtt tggcggctgg acttgtgcgt
     5761 tgcgttctgc cttctcccga cgtgtcgggc ttggaacgca ttgagaggcc tattcgtttg
     5821 cggtcgatat ctatgctcgt cacggtcact tcgacttttt gatgaacttt taccacgtcg
     5881 gacggttttt ccacgaacct gtcggccagt tcgcttatgt gtaccagtcc gtcctgatgc
     5941 acgccgatat ccacgaatgc gccgaagttt gttacgttgg tgacaacgcc aggaagtttt
     6001 tggccgattt taaggtcggt catttccttg atgccgtcgg cgaaggagaa gagctggaac
     6061 ggctctctcg ggtcacggcc gggtttagcg agttcctgca aaatgtcgtt gagagtcggc
     6121 aggccgatgc tgtctgtgac gtatttttta aggtcaatct ttttacgaag ttcgtcgttg
     6181 gccatcaggt cgcctaccgt gcagccgagg tcagcggcta tccgttccac tatcggataa
     6241 ctttccggat ggacggcaga cgcgtccagc ggattattgc cgctgcgtat gcgtaaaaag
     6301 ccggccgcct gttcgaacgt tttagggccc atgcctgcga ctttcagaat ctctcgccgg
     6361 tcggtgaacg ggccgttttc atttcgcgtt tccacgatag ctgccgcgat tttttcgctt
     6421 aagccggcga cgtgcgacaa caactgcttg cttgccgtgt ttatttccac gccaacggaa
     6481 tttacgcagc ttgtaacgac gtcgtccagc gagtttttga gttttttctg gtctacgtcg
     6541 tgctgatatt ggccgacgcc gattgatttc gggtctattt tgaccagttc cgccaacggg
     6601 tccatcagcc gcctgcctat cgaaacgctg ccgcgcacgg ttacgtcctt gtcaggaaat
     6661 tcgtcgcgtg cgacttccga tgccgaatag acgctcgcgc ctgattcatt gaccatgacc
     6721 accggcagag gccgtccgaa atctattttg tcgcagaatg cctttgtttc cctgcccgcc
     6781 gtgccgttgc cgattgcgat agcctgtatc cggaattttt ccgccagcac gcgaatcttc
     6841 atttccgctt cgtcttcctt atgcaggtag attacgtcgt cgaacagcaa tttgccctgc
     6901 gggtcgagac ataccagctt gcagccggta cggaagcccg ggtcgagcgc gagtaccgat
     6961 tgctgtccca gcggcgatgc cagcagcagt tcgcgtatgt tatccgcgaa aaccctgata
     7021 gcctcttcct cggctcgatt tttcatttca agtcgcattt ccgtttccat cgacaatgac
     7081 aacagccgtt taaaactgtc ttccgccgca gcctgtacct gcatggccgc ggcgctgttg
     7141 ccttttacga acatgcgaaa cagtatgtcg attgcctgct gttcgtccgg ctgaatatgg
     7201 aaggtaagga aaccttcttt tgctccgcga cggatggcga gtatgcggtg cgacggcgcg
     7261 ccggcagccg gctcggccca ttcgaaataa tccgaatatt ttatggcgtc ttcttccttg
     7321 cccttgagca cttttgacga catgacggaa tttttttcgt aaagtttgcg aagttctgcg
     7381 cgggcggcgg cattttccga aatattttct gcgatgatgt cgcgtgcgcc ggctaatgcg
     7441 gcggctacgt cgggcacttc acggtctttt tccgtcgata tatatttctg cgcctcagct
     7501 agcgggtctg tggccggatt ctgctcgagc agcagcaggg cgagtggttc aaggcctttg
     7561 tctttagcca tcgttccgcg ggtgcgacgt ttgggcttat atggaagata gatatcctcc
     7621 agaatgttca ttgtttcagc agcctggatt ttcgcttcca gctcgccggt cagtttgccc
     7681 tgttcttgta tggatttaag aatagcctgg cgacgtttgt ccagctcctg caactggtcg
     7741 agacggtcgc ggatggcgat gatgtttact tcgtcaaggc cgcccgtgcg ctccttgcga
     7801 tagcgggcga tgaacggcac tgtgccgcct tcggcgaaaa gttcggcgac agactgcact
     7861 tgcgacggat ttatagactg ctccagggct atcttgcgga aataattggc cagttcgaga
     7921 acttccatgg tcatgattca acaaatcctt tcattgcaaa cagcattaaa acgtacattt
     7981 atgaagcgga gattttactt gtctgctgcc gccgtttcca gtttttttgc aagtggatat
     8041 tttgattttg aatgcgtaga atatcaaaat ggtttttgat ttttttgaca aaaaagggtt
     8101 atgcgtgctt gttggcgccg gcgtcggcgg gcgtgagatg ctcacggctg acggcgaata
     8161 ctggttaggc cgggccgacg tggtgattta cgaccgactc gccgatgatt cactgcttga
     8221 actcgtacgt agtgatgctg aaatgattta cgtcggcaaa caagccggcg accataccat
     8281 gccgcaagag cagataaacg ctcttctggt taaatatacg ctgg
//
LOCUS       DAONFI010000068         8317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_9912_length_8317_cov_4.072259, whole genome shotgun sequence.
ACCESSION   DAONFI010000068 DAONFI010000000
VERSION     DAONFI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9912_length_8317_cov_4.072259"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1155
                     /gene="ald"
                     /locus_tag="PLK08_03375"
     CDS             46..1155
                     /gene="ald"
                     /locus_tag="PLK08_03375"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPS52573.1"
                     /translation="MSQIGVPKEIKTHEYRVGMTPECVRAYLSRGHVVTVQAGAGLGA
                     GFSDEEYTSAGAKICPKAADVYSSADMIVKVKEPVASEYGLFHSGQILYTYLHLAADK
                     PQAMALLAAGVKAVAYETIELADGSLPCLKPMSEIAGRLSVQEGAKYLEKPFGGRGVL
                     LGGVPGIKPGRVVVLGGGVVGTNACKMAVGLGADVTVLDVSARRLEYLDDIFGGRLKT
                     LYATQANIAEEVKTADLVIGAVLLAGAKTPNLVKREMLATMNKGAVIVDVAVDQGGCV
                     ETTRPTTHDDPTFVVDGVVHYCVANMPGAVARSSTLALTSTTLDFGLQLADNGVEKAC
                     RESCAIRKGLNIYCGRCTCEPVACALNIEYDNPTF"
     gene            complement(1330..1953)
                     /locus_tag="PLK08_03380"
     CDS             complement(1330..1953)
                     /locus_tag="PLK08_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPS52574.1"
                     /translation="MSQLNELTDAIKGGKNNQAKELVEALLAAKAKPAEILGSLIAGM
                     DYIGQKFKNNEVYVPEVLIAARAMKTAMGILEPVLVAAGIRPEHKVVIGTVKGDLHDI
                     GKNLVAMMLKGANFEVIDVGVDVTPEKFIAAAKDNGADIVAMSALLTTTMPFMRETVD
                     AIKKAGLSVKTMIGGAPVTQEYADEIGANGYSSDAASAVDLAKKLCA"
     gene            complement(2178..3311)
                     /locus_tag="PLK08_03385"
     CDS             complement(2178..3311)
                     /locus_tag="PLK08_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS52575.1"
                     /translation="MMSVSSRHENATWLSGGLRRIRPAEIRIHKMQREYYLNLAAEGL
                     RMPIGADLILHEKSDPQACRLNGEWLGEILVETARKFRTPLAFPLMDLRIEKEWLLRE
                     FGIAQERIDTYHFQDGLTQADVEKVRNMLNSEPTSAMKASLTAISHVAHTDNLVPVGM
                     CIGPFSLMTKLIDDPITAVFLASLDATEKEAVSVFSALELGTLLICKWIELQIAAGAK
                     AICVCEPAYNTVYISPNQFKANPQLLDDLVIRYNNRMRDVMRKHDVDLIFHDCGALTE
                     EIIASCNKLDPAILSLGSPSDMTLAAKFTQPQTVLFGNLPSKKFYSDNEMTQEQVRQQ
                     SRLLIKQMATAGHPFILATECDVLSVPGCEQTIMKKVMAMLDA"
     gene            complement(3390..4052)
                     /locus_tag="PLK08_03390"
     CDS             complement(3390..4052)
                     /locus_tag="PLK08_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52576.1"
                     /translation="MDEVKISQLKSRGIMLQRDGVTFSVRIGTTGGRFTAAELRDTAN
                     LCERFGHGEVHITTRQAVEIHHVPENRIDEFISAYEATALTPARSGTRVRTVVACPGN
                     KVCKFAGIDSQLMAHEIERRFGDIAILPSKIKIAVAGCKNCCTRANYNDIGIIGAGKN
                     RYELFVGGHGGRTQQAGKSCGIYDSPEKLFDAINDLIRKYASEAAPKQRIGQWLNAET
                     KS"
     gene            4557..5867
                     /locus_tag="PLK08_03395"
     CDS             4557..5867
                     /locus_tag="PLK08_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPS52577.1"
                     /translation="MCTSGFASAEDSVNVVNFSQAIDTVWVLIAAFLVFFMQAGFGMV
                     EAGFIRAKNSCNILTKNFLDFCIASITFFLVGYAFMFGKDVSGVIGTSGFALDAGSLN
                     PSGVPIWAFWLFQCAFCGAAATIVAGAMAERMKFPAYLIYTTVISAFVYPIIGHWVWG
                     GGWLASLGFQDFAGSTVVHATGGWAAFMGAWLLGARIGKYTPDGSAHALPGHNIPLAA
                     LGVFILWFGWFGFNPGSTLSMTGEGMAELAARVALNTNISAAAAAVSAILTVWVFYGK
                     PDLSMAMNGALAGLVAITAGCAFVQPWAAIVIGLVGGVLVVLGVVVLDKLHVDDPVGA
                     VPVHGVNGLWGTLAIGLFGTSQVNGALPHEGLFYGGGWTQFGIQSLGAFTCIAFTLVT
                     MGLLFFAIKKTIGLRVSREEELRGLDIGEHGMEAYAGFQIFTNV"
     gene            5903..6244
                     /locus_tag="PLK08_03400"
     CDS             5903..6244
                     /locus_tag="PLK08_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPS52578.1"
                     /translation="MKLIIAYIQPERLNDVKQSLYKAEIHKMSVCNVMGCGQQMGYKE
                     TYRGVGMEVNLLKKVRLEIAVNDEYVKPTIDAIIDGARTGNIGDGKIFIISLDECIRI
                     RTGETGGEAIG"
     gene            complement(6332..>8317)
                     /locus_tag="PLK08_03405"
     CDS             complement(6332..>8317)
                     /locus_tag="PLK08_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPS52579.1"
                     /translation="NFRSGGDVIDWVNEIFGVGSEIFFGSNATDMQAEAESMHLGRDD
                     TEIGPPADINGVYSLTIDQPGNVTEAAVEEESGKIAAFIRDAIDTGKTIVDFRRDGAA
                     YTRAVKAGDFMIITHNKNHMAVYARALNERNLPAQVTGGGELNGTPEIKWLLDCLRAV
                     SRPIDSVALVASLRSELWGFSDADLLTYKRAGGVFNYRTALPDSLPQKVREKFSAAFA
                     SMKIFSHWLDGEYPPVVVVEKMLTELGLLMRGAARQGGMGNVGAMCRLLELLRNRQRD
                     NCSAAELVNMLQDIVDGAVKFDTIPPRPLTESPVRVMNLHKAKGLQAQIVFLACPRGR
                     RKHDVTSHIHRGEDGVKGFMAFFRETAFGQAAIAFPSGWSDAGGWNDIEGGFLEAEQV
                     RLLYVAATRAGRALVVSTHPKSLTVNPWNPFREMLKKELPLPKISEQADIIQKTAASE
                     NLCDINVASLETQIAAKKAILRQPTFTLHAAKSLAMNQSADNDNIANFFGESDSMGQN
                     WGSAVHRLLELYMRNDGIDLNVAAAEAANQYELDSSIVTELADTVRSVAHSELWSRAK
                     TAEKCLVEVPFEICKPADGQPMIISGVIDMAFKTPAGWVIADYKTDATDSGNAKAVAE
                     HYRPQMKLYADVWQDITGLPVCETGIYLVAIDRYVTL"
BASE COUNT         1840 a         1973 c         2201 g         2303 t
ORIGIN      
        1 agccggcttt agtgatgagg aatatacttc tgccggcgct aaaatatgtc ccaaatcgga
       61 gtacctaagg aaataaagac gcatgagtat cgcgtcggga tgacacctga gtgtgtcagg
      121 gcgtatttat cgagaggtca tgtggtgacg gttcaggctg gtgcaggact tggagccggc
      181 tttagtgatg aggaatatac ttctgccggc gctaaaatat gtcccaaagc cgccgatgtc
      241 tattcctctg ctgacatgat agtgaaagtc aaagaacctg ttgcctcgga gtatggtctt
      301 tttcattccg gccagattct ttatacctat ctgcatttgg cggcggacaa gccgcaggct
      361 atggcattgc tggcggcagg cgtgaaggct gttgcctacg aaaccataga attagccgac
      421 ggttctctgc cgtgtctcaa acccatgagt gaaatcgccg gccgtctgag cgtacaggaa
      481 ggcgctaaat atctggaaaa acctttcggc ggccgaggcg tgctgcttgg cggcgtgccg
      541 ggcataaaac caggcagggt agtagtgctg ggcggcggtg tcgttggtac gaatgcctgc
      601 aaaatggctg tcggtttggg agcggatgtg accgttctgg acgtctccgc caggcggttg
      661 gagtatctgg acgatatatt cggcggccgg ctgaagacgc tctacgccac gcaagccaac
      721 attgccgaag aagtaaagac ggcggatctg gtaataggcg ctgtcctgtt ggctggcgcg
      781 aaaaccccga atctggtcaa acgggaaatg ctcgccacca tgaataaagg cgcggttatc
      841 gtcgacgtgg ccgttgacca gggcggctgt gttgagacaa ccaggcctac aacgcatgac
      901 gacccgacat ttgtggtgga cggcgtagta cattactgcg tcgccaacat gcctggagct
      961 gtggccaggt catccacgtt ggctctcacc agcacaacgt tggacttcgg cctgcaactg
     1021 gcggacaatg gcgtggaaaa agcatgcagg gaatcctgtg ctatccgcaa aggtttgaat
     1081 atctattgcg gcagatgtac gtgcgagcct gtagcatgtg cgctgaatat cgaatatgat
     1141 aatccgacat tttaaaatgc tctatcctga aataaaaaac gaacccgtca gcaatatggc
     1201 tggcgggttt gtttttttat agtttctttc aggcggttat tgcccgtttt tgtttggacg
     1261 gattatatag cggctggctg actgattttt gctataaatc aaagttaaca tgctttagcc
     1321 ggaaaattat tatgcgcata attttttggc gaggtcaaca gcgctggccg cgtccgatga
     1381 ataaccgttt gcgccaattt cgtcggcata ttcctgcgtt acgggtgctc cgccaatcat
     1441 tgtttttact gagaggccgg cttttttgat ggcatccaca gtttcacgca tgaacggcat
     1501 tgtagtggtc agaagtgccg acatggcaac gatatccgca ccattgtctt ttgcggctgc
     1561 gatgaatttt tccggcgtta cgtcgacgcc gacgtcgatg acttcaaagt ttgcaccctt
     1621 gagcatcatg gccaccagat ttttgccgat gtcgtgcagg tcgcccttga ccgtgccgat
     1681 tacgaccttg tgttccggcc gtatgccggc agccacgagt actggttcga gtatgcccat
     1741 ggctgttttc atcgcacgtg cggcaatgag aacttccggc acatatactt cattgttttt
     1801 gaatttctgg ccgatgtagt ccatgccggc gataagtgag cccaaaattt ccgccggctt
     1861 tgcttttgca gccagcaaag cctccaccag ttcttttgcc tggttgtttt tgccaccttt
     1921 tatggcgtct gtgagttcat ttaactgact catttttttt ctcctgaatc ctgttgaacc
     1981 gtttttttta tgaacttttc agtagttttt accggcggct gacaagtccg ccgtattgca
     2041 ttttatatcc atgtaaataa aaccgacatc tatatgtaac tatagcacct gttgtaaaat
     2101 aagtctttca aacagagcgc attttatgtc actttcagct tatttcaccg gctatcaggg
     2161 tgctttcggc agctactcta ggcatccagc atggccatga cttttttcat tatcgtctgt
     2221 tcgcagccgg ggacagagag tacgtcgcat tcggtggcga ggatgaatgg atgaccggcg
     2281 gtggccatct gcttgataag cagcctggat tgctgacgta cctgttcctg agtcatttca
     2341 ttatccgaat aaaatttctt ggacggcaga ttgccgaaga gcaccgtttg cggctgggtg
     2401 aatttggcgg ctagagtcat atcgcttggg ctgccgaggc ttaatatggc tgggtcgagt
     2461 ttgttgcagc ttgcgattat ttcttccgtc aacgcgccgc agtcatggaa tatcaggtcg
     2521 acgtcgtgtt ttctcattac gtcccgcatc cggttattat agcggattac gaggtcgtcc
     2581 aggagttgcg gattagcctt gaattgattg ggagaaatat agacggtgtt gtacgccggc
     2641 tcacagacgc agatggcttt tgcgccggct gctatctgca gttcaatcca tttacatata
     2701 agcagagttc caagttccag agcggagaaa accgagacgg cttctttttc ggtcgcgtca
     2761 aggctggcca ggaaaacggc cgttatgggg tcgtcaatga gctttgtcat gagcgaaaac
     2821 gggccgatgc acatgccgac gggcacaagg ttatcggtgt gggcgacgtg agaaatggca
     2881 gtcagcgagg ctttcatggc tgacgtcggc tcagagttca gcatattgcg caccttttcc
     2941 acgtcagcct gcgtaaggcc gtcctgaaaa tgatacgtgt ctatccgctc ctgagctatg
     3001 ccgaattcac gaagcagcca ttctttttca attcgcagat ccatcagcgg gaaagccagc
     3061 ggagtacgaa atttccgagc ggtttccacc aaaatctccc ccagccattc gccgttaagc
     3121 cggcatgcct gcgggtcgga tttttcgtga agtatcaggt ctgcgcctat cggcatacgc
     3181 aggccttccg ccgctagatt aaggtaatat tctctttgca ttttgtgtat ccttatttca
     3241 gccggacgga ttcgtcgtaa tccgcctgac agccatgtcg cattttcatg acgtgatgag
     3301 acgctcatca ttcatgtatg cgataatata catactttag caaagaggta ttgtgcgtgt
     3361 cttgtaatgt cggcgcattt tgtgggattt taagattttg tttcagcgtt cagccattgg
     3421 cctatacgct gttttggtgc agcttctgat gcatattttc gtatcaggtc attgatggca
     3481 tcgaaaagtt tttcaggcga atcgtatatg ccgcatgact tgcctgcctg ctgcgtccgt
     3541 ccgccatggc cgccgacgaa taattcatag cggtttttgc ccgcccctat gatgccgatg
     3601 tcgttgtagt tcgctcgcgt gcagcaattt ttgcagccgg cgacggctat cttgattttc
     3661 gacggcagta ttgcaatgtc gccgaaacgt cgctcgattt catgtgccat cagttgtgaa
     3721 tcgatgcctg cgaacttaca caccttgttg ccagggcagg ctactacagt acgcactctt
     3781 gtgccgctgc gggctggcgt gagcgcagtt gcttcataag cggagatgaa ttcgtcgatg
     3841 cgattttcag ggacgtgatg tatttcgacg gcttgacggg tggtaatatg tacctcgccg
     3901 tggccgaagc gttcgcatag atttgccgta tccctgagtt cggcagctgt aaaccggccg
     3961 ccagtagttc cgattcttac tgaaaaagta acgccgtcac gctggagcat tatgccgcgg
     4021 ctttttaatt ggctgatttt tacttcatcc atttaaatgt ctctcaacaa agtttttgca
     4081 gaattacgta cgttcaacga tagcataatt tagacaaaat tgtggcacga aaaaaaggaa
     4141 catttttgta gtcacaccac gtctttatta catagttgtc gcaatttgtc tattactatt
     4201 acaaaaaaat tttatcatca gcatttcaga acaaacacaa atgtaatttt tttaaaattg
     4261 ggccaatatc acaaaaacgt tgaaattcgg gcgcaaataa catttatgta gcaggttgaa
     4321 ccagtgtcag cgtcatatgt ttatggccaa tttatattac atggcatgac ttgtgcaaac
     4381 ggtaaatgaa taaatatgaa aacttatcag tcttgtgtac gtttatggca gataaagttt
     4441 gccttgtgca gagttgaaat taattttcat gttattgttc aggatatttt tttattagga
     4501 gaagcaaagt gaaatcgaac ggattgtcgt attgcggtgc gttgttgacg gtattattgt
     4561 gtacgagcgg attcgctagt gcggaagatt ctgtaaatgt ggtaaatttt tcgcaggcta
     4621 tcgacacggt atgggttttg atagcggcgt ttctggtgtt tttcatgcag gctggattcg
     4681 gtatggtgga ggcgggcttc attcgcgcca aaaactcatg caacattctt acgaagaatt
     4741 ttttggattt ctgcatcgcc agcattacgt tttttcttgt cggctatgcg ttcatgttcg
     4801 gcaaagacgt ttctggcgta atcggcacga gcggatttgc attggatgcc ggttcgctta
     4861 atccgtccgg cgtgccgatt tgggcgttct ggttattcca gtgcgcattt tgcggagctg
     4921 ccgcaactat cgtcgccggc gcaatggctg agcgcatgaa atttcctgcg tacctgattt
     4981 acacgacagt tatttcggcg tttgtgtatc cgataatcgg ccactgggtc tggggcggcg
     5041 gctggctggc tagcctcggt ttccaggact ttgccggttc gactgtcgtt cacgccacag
     5101 gcggctgggc tgcgtttatg ggagcctggc tgctcggtgc gcgaatcggc aaatatactc
     5161 ccgatggctc agcacatgca ttgcctggcc acaatattcc gctggcagca ctgggagtgt
     5221 tcatactctg gttcggatgg ttcgggttca atcctggcag taccttgagc atgacgggcg
     5281 agggcatggc tgaactggcg gcacgcgtgg cacttaacac gaacatatct gccgccgccg
     5341 cggctgtctc ggccatactg acggtgtggg tattttacgg caagcctgat ctttcgatgg
     5401 caatgaatgg cgcgttagca ggtctggtgg cgataaccgc agggtgtgcc tttgttcagc
     5461 cgtgggctgc tatcgtgata ggcttggtcg gcggcgtatt ggtggtgctg ggtgtggtag
     5521 tgcttgacaa acttcatgtg gacgatccgg tgggtgcagt gccggttcat ggcgtcaatg
     5581 gcctttgggg tactttagcc atagggttat ttggaacttc acaggtaaat ggcgctctgc
     5641 cgcatgaagg ccttttttac ggcggcggct ggacgcagtt cggcattcag tcacttggtg
     5701 cgtttacgtg tatagccttt actctggtca caatgggcct gcttttcttc gcaatcaaga
     5761 aaacaatcgg cttgagagtc agccgcgaag aagaactccg cggtttggac attggcgagc
     5821 acggcatgga agcatatgcc ggcttccaga ttttcaccaa tgtttaaggc tgctataatg
     5881 taatgaacga caggagttaa aaatgaaact tataatcgca tatattcagc ccgaaaggct
     5941 taacgacgta aagcagtcgc tgtacaaggc tgaaatacac aaaatgtccg tctgtaacgt
     6001 tatgggttgt ggccagcaga tgggctacaa ggaaacatat cgcggcgtcg gtatggaagt
     6061 gaatctgcta aaaaaggtac ggctggaaat tgccgtcaat gacgaatatg tcaaacctac
     6121 cattgatgcc ataatcgacg gtgcccgaac gggtaatatc ggcgacggaa agatttttat
     6181 aatatcgctg gatgaatgca tacgtatccg taccggcgaa acaggcggcg aagcaatcgg
     6241 ctaaaaattt ttttgagaat cgccgctctg aaagtccgcg aatttgcgta tgcgtaagga
     6301 tttttatcgc agattttacg aaggccgtat tttacagcgt tacgtatctg tcgattgcca
     6361 cgagatatat gccggtttca cagaccggca ggcctgttat atcctgccag acgtcggcat
     6421 aaagtttcat ctgtggcctg taatgttcag caacggcttt tgcgttgcct gagtcggttg
     6481 cgtcggtctt ataatccgcg atgacccagc ctgccggcgt tttgaatgcc atatcaataa
     6541 cgccgcttat aatcatcggc tgtccgtcgg cgggcttgca gatttcgaac ggcacttcaa
     6601 cgagacattt ttcggcagtt tttgctcgcg accatagttc cgaatgggct acgctgcgaa
     6661 cagtgtctgc aagctcagtc actatagacg aatcaagctc atattgattt gccgcctcag
     6721 ccgctgctac gttaaggtca atgccgtcgt ttcgcatata cagttccagc agccgatgaa
     6781 cggctgaacc ccaattttgt cccatggaat ctgattcgcc gaaaaaattt gcaatattgt
     6841 cattgtcggc agactggttc attgcaagtg acttggcggc atgcagtgtg aatgtcggct
     6901 gacgtaatat cgcttttttc gcagcaatct gcgtttcgag tgatgctaca tttatgtcgc
     6961 acaaattctc tgatgccgcc gttttttgga taatgtcagc ctgttcagat attttgggta
     7021 gcggcaattc ttttttgagc atttcacgga acggattcca cgggtttacc gtcagcgatt
     7081 ttggatgcgt gctgactaca agcgcacggc cggcgcgtgt cgcggctacg taaagcagtc
     7141 gaacctgttc cgcttcgaga aaaccgcctt caatgtcgtt ccagccgcct gcgtcgctcc
     7201 agcctgacgg gaatgcaata gcagcctggc caaaagccgt ctcgcggaaa aaggccataa
     7261 aaccttttac gccgtcttcg ccgcgatgaa tatgacttgt gacatcatgt ttgcgtctgc
     7321 cgcgcgggca tgcaagaaaa acaatttgtg cctgcagccc ctttgccttg tgcagattca
     7381 ttacgcgcac aggcgattcc gtcagaggcc gcggcggtat tgtgtcgaat ttgactgcgc
     7441 cgtcgactat atcctgcagc atgtttacga gttcggcggc tgagcagtta tcacgctggc
     7501 gattgcgaag gagttcaagc agccggcaca tcgctccgac gttacccatg ccgccctgtc
     7561 ttgccgcgcc tcgcattagc agccccagtt ccgtaagcat tttttctacc actaccaccg
     7621 gcggatattc tccgtccagc caatgtgaaa aaattttcat tgaggcgaaa gcggctgaaa
     7681 atttttctct gaccttttgc ggcagggaat caggcagggc ggtgcgatag ttgaaaactc
     7741 ctccggctcg tttatacgtc agcaggtctg cgtctgaaaa tccccacagc tcgctgcgga
     7801 ggctggccac gagtgcgact gaatctatgg gtctgcttac tgcccgcagg caatcgagca
     7861 gccatttgat ttccggcgta ccgttcagtt cgccgccgcc tgtcacctgt gcgggcagat
     7921 tccgttcgtt gagagcgcgg gcatagacgg ccatgtggtt tttgttatgc gttattatca
     7981 tgaaatcgcc ggcttttacg gctcgcgtat atgcagcgcc gtcgcggcgg aagtcaacta
     8041 tggtttttcc ggtgtctatt gcgtcacgta tgaatgccgc gattttgccg gactcttctt
     8101 caacggccgc ttctgtaaca ttgcctggct ggtctatcgt cagcgaatag acgccgttga
     8161 tatcagccgg cggcccaatt tccgtatcat cgcgaccgag gtgcatcgac tccgcttccg
     8221 cctgcatgtc tgttgcatta ctgccaaaaa aaatttcgct gccgacaccg aaaatttcat
     8281 tcacccaatc tataacatcg ccgcctgaac ggaaatt
//
LOCUS       DAONFI010000069         8097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_10287_length_8097_cov_4.007958, whole genome shotgun sequence.
ACCESSION   DAONFI010000069 DAONFI010000000
VERSION     DAONFI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10287_length_8097_cov_4.007958"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLK08_03410"
     CDS             <1..750
                     /locus_tag="PLK08_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52580.1"
                     /translation="SYHHSAGGVTTGFADLLCMLSPIVGLRRLRFVTSHPMSFDENIL
                     TAMRDLPNVCHYMHVPAQSGSDVMLKAMNRGYTSAVYDELICTARSIVPDMVFAGDFI
                     VGFPGETDEDHAASAELIRRTGYRNSFIFKYSPRPGTLAAKKYADDISEETKKNRNNE
                     LLAVQKEVSLVHHEAFVGRTLEVLVTGPSRRSDRQDSPGTARRMQMMGRTRGDHIVIF
                     DGPESSTDTYRDVTITSAGELILFGELNPLA"
     gene            971..3094
                     /locus_tag="PLK08_03415"
     CDS             971..3094
                     /locus_tag="PLK08_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPS52581.1"
                     /translation="MNISDAENLQDDSAGNNNDACSGKFMREFKNLMTSRRIMPLWVL
                     AAESYAIFFIFRLLLFLFSLVAMKKVSVREIVHCFWVGLGFDSMVVGYIVLPLAVLLT
                     ISRAWIFERNWFHRLITCYLTLMTIYLLFIETVGFFYFLHYGQRINWIAPYYLLSPHE
                     VFGYIFRVYPWAYLSFIMFPVLFYFLYTFFRNLFWGKSVPVLPGWRRPALVAAVLLLC
                     IIACRGGFGNRPLNMATVYKDTHNQLLAELAKNHSYTLFYGTKNLIDDRNSDEKEMFG
                     LMSHRYAAPVAVRMLYQADDQPLHDTANPLWRRTDTGRKRLDCNVVVIVMESMAGELI
                     GALQYPNAKSCSPNLDRLCREGLFLSRLYAVGARTSRGMVGTLCGYPDLGWKTVLMRP
                     NAIGHFLTLPTFFKKRGYETAFVYGGQMSFDNMGPFFGAGGVGKMIGQEQITATQPGS
                     SWGWPDEFIFNKALTTFDDMAKRDKRFFGIILTVSNHPPFDVPDGRVKMLPLDDDANR
                     IKNATRYADWALGRFFDEASRKPWFRDTIFVLVADTGREVAFDKTKIIDAVSFRVPCL
                     FYSPANIKPQVLDTIASQTDIAPTLLSMLGGSFEHCFLGRNILEVPKNDGFALLREDD
                     RMGFIYGDSLVIQPPRSRPILYNLDKFAVNEKYDPAGVETLGVLTRMHAIYGMAKYLY
                     DKGIYNDPKLTANKRGNSNEKRKEH"
     gene            3094..3624
                     /locus_tag="PLK08_03420"
     CDS             3094..3624
                     /locus_tag="PLK08_03420"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPS52582.1"
                     /translation="MSKVDIASYIRDVRDFPKPGIVFKDITPLLCSHAALDETVSLLL
                     SPFANEKIDLVCGIESRGFIFGSLIANRLGVGFVPIRKAGKLPAETLEESYELEYGRA
                     TIEIHTDAVKPGQRVLMVDDLLATGGTMAAACRLVERLGGEVVGCPFVIELTFIPGRK
                     ALAKYRVHSLLKVTGE"
     gene            3643..5184
                     /locus_tag="PLK08_03425"
     CDS             3643..5184
                     /locus_tag="PLK08_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPS52583.1"
                     /translation="MISRIHSAILQGIDASACEIEADVSKGGVGEIKLVGLADASVRE
                     STSRIQAAMRNSGYRWPGPKVTINLAPADVHKDSAAFDLPIAVACMLAGGIFQSDLAD
                     EYLVMGELALDGRLRAVRGALAVAMLAQSLGKRGVILPADNAREAAVVAGVDVIPVKY
                     LSDAVGFLGGVLPLDAACEDIEKIFNVDSAYDMDFADVRGQESAKRALTVAAAGHHNI
                     LLIGPPGSGKTMLAKRIPTILPPLTVEESLETTRVYSARGLLSAGQSLMTRRPVRTPH
                     HSSSSAALVGGGAIPQPGEVSLAHHGVLFLDEFPEFARTTLEMIRQPLEDGCVTIPRV
                     HSTIKFPAQIMLVAAMNPCPCGYYTDPKRPCMCSPSQVERYLSRVSGPLIDRIDIHVE
                     VPPVDWKELRASNGGMTSEEMRKAVCEARQIQRKRFSKAKNAPSTTTNATMNSKQLRE
                     YCKLNGECEMLLRQAMTELGLSARAHDKVLRIARTIADIEKSDDIAPHHIAEAIQYRR
                     LDRKL"
     gene            5322..6623
                     /locus_tag="PLK08_03430"
     CDS             5322..6623
                     /locus_tag="PLK08_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52584.1"
                     /translation="MNKLLLFWMVMIIGFGAGFARADSNSNTDESSWRSSDLGVSSFG
                     EQWSGPAMPKEQLEGKVVWVTILKSSSSDVKTLKDMAQLDRLYRRKGAVIIAIHYIMT
                     TKSKALDNVSRADARFPVYENARVTYKGRDRKGVTNTRNAYRLPYAILYGADGMIAWE
                     GPCMPAQSQMAAILQRELKKMPKKASLQDDNTDDESSSIDELFDDLDTSRIADIESLV
                     KKHRMGQAIAKCETSKDADNDRTSQAKELLGRLKAYASQQMAAAKEQRKILPSKMVSM
                     LTEIRRDFSGTEYAEEASELLDKCRKDKEFQSDLDAERSYQSVLKYSESIPVPEQGEN
                     YGEWQKKNRRRLDALKRKVDAFKDKNSQSMFCKKLDDLLKDYEEKKVSDVETNNAKVK
                     PVVEKVDNVEDSVKDTTTSAAEELLKEMKLDSEDSGNKTKE"
     gene            complement(6769..7302)
                     /gene="nuoE"
                     /locus_tag="PLK08_03435"
     CDS             complement(6769..7302)
                     /gene="nuoE"
                     /locus_tag="PLK08_03435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPS52585.1"
                     /translation="MNQCADSKTDNRQQVQADSQAALTPEIVTLIEQCRKEPHSESQL
                     IKVLHKVQEVFGYLSPKTLDAVAQLLQVPSSTVSGVATFYHFFRTVPKGKHTINVCLG
                     TACYVKGGQAIADKFREELGIEFGETSGDGMFSLNAARCLGTCGLAPVVMIDEEVHGQ
                     VTPEQVPALLDKYRNQP"
     gene            complement(7299..>8097)
                     /locus_tag="PLK08_03440"
     CDS             complement(7299..>8097)
                     /locus_tag="PLK08_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPS52586.1"
                     /translation="MSVLIKTYYAEKAGISPEKIYVVGIMPCVAKKYEAARPEHMMKN
                     GQPYTDAVLTTRELIWMIKSFGIEYWSPADMDAKKKLPNMEFDAPLGIATGAGDIFGC
                     TGGVMEATLRTAYEKLTGKPCEKLEFYDVRAVEGLRQATLDIAGRKINIGVANGLVNA
                     KTLLDKVISGEKQFHVIEIMACPGGCIGGGGQPYPAHRSKVRDAELMRLRAQALYSID
                     ERKTLRKSHENPAIIELYKNYLGDIGGKMAHELLHTHYSAKLPRGIK"
BASE COUNT         1889 a         1921 c         2205 g         2082 t
ORIGIN      
        1 agctatcatc attcagccgg cggcgtgacg acgggttttg ccgacttgtt gtgcatgctt
       61 tctcctatcg tcgggttgcg gcgtctgagg tttgtgacga gtcatccgat gtcgtttgac
      121 gaaaatatct tgacggctat gcgcgacctg cccaatgtct gtcactatat gcatgtgcct
      181 gcacagagcg gttctgatgt catgctcaaa gccatgaatc gcggatatac ctccgccgtg
      241 tatgacgaac tcatatgtac agcccgttcg attgtgccgg atatggtttt tgccggcgat
      301 tttatcgtcg gttttcctgg cgagacggac gaagaccatg ccgcgtcggc agagcttatc
      361 cgtcgtaccg gttacagaaa ttcgttcatt ttcaaatatt cgccgcggcc gggtacgttg
      421 gcggctaaaa agtatgccga cgatatttcg gaagaaacca aaaaaaatcg caacaacgaa
      481 ttgttggctg ttcagaaaga agtttcactt gtacaccacg aggcgtttgt cggcagaact
      541 ctggaagttc ttgtaaccgg gccaagccgc cgcagtgacc gtcaggattc acccggaacc
      601 gcccgacgca tgcagatgat gggcagaacg cgcggcgatc atattgtaat tttcgacggc
      661 cctgagtcgt cgaccgatac ataccgcgat gtgaccataa cttctgccgg agaactcatc
      721 ctgttcgggg aattgaatcc tttggcttga cggcggctgt taattgcagg cagccagggt
      781 gtttggggta ggttctaata aagtttcgca tatttcttgc aaacaggctg cttttgtaaa
      841 taataatgaa aatggttttt gacgttacat tgcgtcagcc gtacgacagg tgtgattttt
      901 gagtgatata aacgcaaggc ctgtcatttt ttgggaaaat tgtcatttat cagaatgcga
      961 atgctgtcga atgaacattt ccgatgctga aaatctgcaa gacgactctg ccggcaataa
     1021 taatgatgcc tgcagtggta aattcatgcg cgaattcaag aatctcatga ccagccgccg
     1081 gataatgccg ctgtgggtgc tggctgcgga atcttatgcg atttttttca tattccggct
     1141 gttgcttttc ctgttttcac tcgtcgccat gaaaaaagtc tccgttcgcg agatagtgca
     1201 ttgtttctgg gtggggcttg gctttgacag tatggtagtc ggctatattg ttcttccgct
     1261 ggcggtactc cttacgatat cgagagcctg gatttttgaa cgcaactggt ttcatcgtct
     1321 aattacatgt taccttacat tgatgacgat ttatctgctg tttattgaga ccgttggttt
     1381 tttctatttt ctgcattacg gccaacgtat caactggata gcgccgtatt accttctcag
     1441 cccgcatgag gttttcggat atattttcag ggtttatccc tgggcgtatt tgtcttttat
     1501 catgtttccg gtgctgttct attttctcta tacatttttt cgcaatcttt tctggggcaa
     1561 gtcggtgcct gttctgcccg gctggcgacg gccggcgttg gtggcggcgg tgcttctgct
     1621 gtgcataatt gcctgccgcg gcggtttcgg caacagaccc ttgaatatgg cgacggttta
     1681 caaggatacg cataatcagc tgcttgcgga attagccaaa aatcactctt atacgctttt
     1741 ttacggaacg aaaaatctca tagacgaccg gaattccgat gaaaaggaaa tgttcggcct
     1801 catgtcgcac cgatacgccg ctcctgttgc cgtccggatg ctgtatcagg cggatgacca
     1861 gccgctgcac gatacggcta atcccttgtg gcgaaggacg gataccggca gaaaacggct
     1921 tgactgcaat gtcgtggtca tagtcatgga gagcatggct ggcgaactta tcggcgcatt
     1981 gcaatatccg aacgcgaaaa gctgctcgcc gaacctggac agactctgtc gcgaaggact
     2041 gtttttaagc cgtctgtatg ctgttggcgc acgtacgtcg cgcggcatgg ttgggacgtt
     2101 atgcggctat cccgacctcg gctggaagac tgtacttatg cggcctaatg cgatagggca
     2161 tttcctgact ctgccgacgt tttttaaaaa acgcggctat gaaacggcgt tcgtttatgg
     2221 cggccagatg tcgttcgaca atatggggcc gttcttcggt gctggcggcg ttggaaaaat
     2281 gataggccag gaacagatta cagctacgca gccgggcagc agttggggtt ggccggacga
     2341 atttattttc aacaaggcac tgactacttt tgacgatatg gcgaaaaggg ataagagatt
     2401 tttcggcata attctgaccg tatccaacca tccgccgttt gacgtgcctg acggcagagt
     2461 caaaatgctt ccgctcgacg acgatgccaa ccgcataaag aacgccacgc gatatgccga
     2521 ctgggctctg ggccgttttt ttgacgaggc atcccgcaag ccgtggtttc gcgataccat
     2581 cttcgtgctt gtcgccgaca ccggcaggga agtggcgttc gacaagacga aaataatcga
     2641 tgccgtgagt ttccgtgttc catgtctttt ctattcacct gctaacatca agccgcaggt
     2701 cctcgatacc atcgccagtc agaccgacat tgcccccaca ctgctttcga tgcttggggg
     2761 cagtttcgag cactgttttc tgggccgtaa tattctggaa gtgccgaaaa acgacggctt
     2821 tgcgctgttg cgggaggatg accggatggg cttcatctac ggcgacagcc tggttattca
     2881 gccgccgcga agtcggccga tattatacaa tttggacaaa ttcgccgtca atgaaaaata
     2941 cgaccctgcc ggcgttgaaa cgctgggggt tctgacccgt atgcacgcca tttacggcat
     3001 ggccaagtac ttgtatgaca agggcatata caatgacccg aagttgactg cgaataaacg
     3061 tggaaattca aatgaaaagc gaaaggaaca ttgatgtcga aagttgatat tgcatcatat
     3121 atacgcgatg ttcgtgattt tcccaagccc gggatagttt tcaaggacat aacaccgctg
     3181 ttgtgcagcc atgctgcttt ggatgaaacc gtgtctcttc ttctttcgcc gtttgcgaat
     3241 gagaaaatcg acctggtttg cgggattgaa tcccgcgggt tcattttcgg ttcgctgatt
     3301 gcgaaccgtc ttggggtagg ttttgtgccg atacgcaaag ccggcaaact tcccgctgaa
     3361 acgcttgaag aatcatatga gcttgaatat ggccgtgcga cgatagaaat acataccgac
     3421 gctgtaaaac ccggccagcg tgtactgatg gtcgacgacc ttcttgcgac cggcggaacg
     3481 atggctgccg cgtgtcggct ggttgagcgg ctgggcggcg aagtggtggg ctgtccgttc
     3541 gtgatagagt tgacgttcat tccaggtcgc aaggcgctgg caaaataccg tgttcactct
     3601 ttgctaaaag ttacgggtga atgacggaga gatttttatc aaatgatttc cagaattcat
     3661 tctgccattc tgcagggcat tgatgcgtcg gcgtgcgaaa tagaggctga tgtgtccaag
     3721 ggcggcgtag gcgaaataaa acttgtcgga cttgcggacg catcggtacg tgagtcgacc
     3781 agccgcatac aggctgccat gcgaaacagc ggatatcgct ggcccggccc caaagtgacg
     3841 ataaatctgg cgccagccga tgtacacaag gattcggcag cctttgacct gcccatagcc
     3901 gtcgcatgca tgcttgccgg cggaattttc cagagtgacc tggcggatga atatctggtt
     3961 atgggcgagc ttgcgctgga cggcagactt cgtgcggtac gtggggcgtt ggctgttgcc
     4021 atgctggctc agagtctggg aaagcgtggc gtgattctgc cggctgataa cgcgcgggaa
     4081 gctgcggtcg tcgcaggtgt tgacgtcata cctgtaaaat atctttcgga cgcggtgggc
     4141 tttcttggcg gggttctgcc gcttgatgcc gcctgtgagg atattgaaaa aatctttaat
     4201 gtcgattcgg cctatgacat ggattttgcc gatgtacgcg gccaggagtc agccaaacgc
     4261 gcgttgacgg tggctgccgc cgggcatcac aacattctcc tgataggccc gccaggcagc
     4321 ggcaaaacta tgttggcaaa acgcattccc accatacttc cgccgcttac ggtggaagaa
     4381 tcgcttgaaa ccacgcgcgt atattcagcc cgcggccttc tttccgccgg ccaaagcctg
     4441 atgacccgtc ggccggttcg cacgccgcat cattcgagtt cgtcggctgc tcttgtcggc
     4501 ggcggtgcga ttccgcagcc tggcgaagtg tcgctggcgc accacggcgt tctgttcctc
     4561 gacgaatttc ctgaattcgc cagaaccacg cttgaaatga ttcgtcagcc gctcgaggac
     4621 ggctgcgtta cgataccgcg cgtccacagt acgataaaat ttcctgcaca gattatgctc
     4681 gtggctgcga tgaacccgtg tccatgcggt tattataccg atcccaagcg gccgtgcatg
     4741 tgttcgccga gccaggtcga gcgatatctt tcccgcgtaa gcggcccgct tatcgaccgt
     4801 attgacattc acgtcgaagt gccgccggtg gattggaagg aacttcgcgc gtcaaacggc
     4861 ggcatgacca gcgaggaaat gcgcaaagcc gtctgcgagg cacgtcaaat acagcgcaaa
     4921 cgcttttcaa aagcgaaaaa cgccccctca accaccacca atgcgacgat gaattcaaaa
     4981 cagctgcgcg aatattgcaa gctgaacggt gaatgcgaga tgctgctgcg gcaggcgatg
     5041 accgaactgg gcctcagtgc cagggcgcat gacaaggttt tgagaatcgc gcgcactatc
     5101 gctgatatcg agaaatctga cgacatagcg cctcatcaca tcgccgaagc tatccagtat
     5161 cggcggctgg acagaaaatt ataaacctct tttctgcccg gatacgatat ttggctttaa
     5221 attcagatac gatatgtgta gaattaatca tgggatgttt tattttgaat ttgaatgaat
     5281 gtatgagcag tatgttcata aattcggtgg gagatattct tatgaataaa ttgctgttat
     5341 tttggatggt tatgattata ggtttcgggg ccggctttgc aagggcagac tcaaattcca
     5401 acacggatga atcgtcgtgg cgcagttccg atctgggcgt atccagcttc ggcgaacagt
     5461 ggtcaggccc ggctatgccg aaggaacagc ttgagggcaa agtcgtgtgg gtgacaattc
     5521 tgaagtcgtc gtcctccgac gtaaagacgt tgaaagatat ggcccagctt gacagattgt
     5581 accggcggaa gggcgcagtc atcatagcca ttcactatat catgacgacc aaaagcaagg
     5641 ctcttgacaa cgtgagccgt gccgatgcgc gtttccctgt ttacgagaat gcccgtgtta
     5701 cctataaagg ccgtgacaga aaaggcgtca cgaatacacg aaacgcatac cgtctgccgt
     5761 atgccatact ttatggcgcc gatggaatga ttgcatggga aggcccgtgt atgcctgcac
     5821 aatcgcagat ggcggcgata ctgcaaagag aattgaaaaa aatgcctaaa aaggctagcc
     5881 tgcaggatga taataccgat gacgaatcat catccataga tgaattgttc gatgaccttg
     5941 atacttccag aatcgccgat attgaatcgc tggtgaagaa acacagaatg ggacaggcaa
     6001 ttgcaaaatg tgagacatcg aaagatgccg ataacgacag aacttctcag gcgaaagaat
     6061 tgctcggcag gctgaaggct tacgcctcgc agcagatggc tgcggctaag gaacagagaa
     6121 aaattctgcc gtcgaaaatg gtttcaatgc tgacggaaat caggcgggat ttttccggaa
     6181 ctgaatatgc cgaagaagca tcggaattgc tcgataagtg caggaaagat aaggaatttc
     6241 agtcagacct tgatgccgaa agatcttacc agtcggtatt gaaatattcc gaaagcattc
     6301 ctgttcctga acagggcgaa aattacggtg agtggcagaa aaaaaaccgt cgccggctgg
     6361 atgctctaaa aagaaaagtc gatgctttta aagataaaaa ttcacagagt atgttctgta
     6421 aaaagcttga cgacttattg aaggattatg aggaaaaaaa ggttagcgac gtcgaaacca
     6481 ataatgcgaa agtaaagccg gtagtggaaa aagttgacaa tgtcgaggat agtgtcaaag
     6541 atacgactac cagtgcggcg gaggaacttc tgaaggaaat gaagttggat tcagaagatt
     6601 cgggaaataa aaccaaagaa tagggcggct ctcaggaatt tttaatatgt ccttcgtaat
     6661 tctacggttt ttaaaccgta gatgaaagta tccgccgtga ggcggacagg cgaaagcctg
     6721 atgaatatga tgctacgggt tttaacccgt agagattcac tttttggctt acggctgatt
     6781 gcgatatttg tccagcagtg ccggtacctg ttcaggtgtg acttggccgt gcacttcttc
     6841 gtctatcatc actaccggcg ccaagccgca tgtgccgaga catctggcgg cgtttaggga
     6901 gaacatgccg tcgcccgacg tttcgccgaa ttcgattcca agttcttcgc ggaatttatc
     6961 tgcgatagcc tgcccgcctt tgacgtaaca tgccgtaccg agacagacgt tgattgtatg
     7021 cttgcctttg gggactgtac ggaaaaaatg gtagaacgtc gccacgccgc tgactgttga
     7081 cgagggcacc tgcaataatt gtgcgacggc gtccagggtt ttgggcgaga ggtatccgaa
     7141 aacttcctgt actttgtgaa gtaccttgat tagctggctt tctgagtgag gttctttcct
     7201 gcactgttcg atgagggtga caatttccgg tgtaagcgcg gcttgggagt cagcctgtac
     7261 ctgttgacga ttgtcggttt tgctgtcggc gcattggttc attttatacc ccgcggcaac
     7321 tttgccgaat agtgagtatg aaggagttcg tgggccattt tgccgccgat gtctccgaga
     7381 tagtttttat acagttcgat tatagccgga ttttcatggc ttttgcggag cgtttttcgt
     7441 tcgtcgatgc tgtaaagagc ctgtgccctg agacgcataa gttcggcgtc gcgtactttt
     7501 gaacggtgag ccggatatgg ctggccgccg ccgccgatac agccgcccgg gcacgccatt
     7561 atctctatga cgtgaaactg cttttcgccg ctgattactt tgtcgagcag cgtttttgcg
     7621 ttgaccaggc cgtttgcgac gccgatattg atttttctgc cggcgatgtc tagggtggcc
     7681 tgccgcaggc cttcaacggc gcggacatcg taaaattcga gtttctcgca cggtttgccg
     7741 gtcaactttt catatgccgt gcgtagtgtg gcttccatga cgccgccggt acagccgaag
     7801 atatcgcctg ctccggttgc aatacccagc ggagcgtcga attccatatt cggcagtttt
     7861 ttcttcgcat ccatatccgc cggcgaccag tattcgattc cgaaactttt tatcatccat
     7921 atgagttcgc gcgtagtaag gacggcgtcg gtgtacggct ggccgttttt catcatgtgt
     7981 tcaggccggg ccgcttcata ttttttggcg acgcagggca ttatgccgac gacgtaaatt
     8041 ttttccggac tgatgccagc tttttccgca tagtaggttt ttatcagcac cgacatc
//
LOCUS       DAONFI010000070         7976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_10502_length_7976_cov_4.297690, whole genome shotgun sequence.
ACCESSION   DAONFI010000070 DAONFI010000000
VERSION     DAONFI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10502_length_7976_cov_4.297690"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLK08_03445"
     CDS             complement(<1..983)
                     /locus_tag="PLK08_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52587.1"
                     /translation="MKIALVNPATRNTSGYHSINTKIPPLGLQVLAKLTPAEHQVEII
                     DEIFNTNDTEELLLSRGYDFVGITSYTSSAPRAYELAVFCRQHNLPAMMGGPHASACP
                     DEASQYFSTVAVGECDAIWHDIIADAQAGCLKPRYEGYLIELTNGLGTADQSMQAING
                     KYDVACIQTARGCPIGCKFCSVTRFNGKQIRRRPVDDIIAEWNTIKKPFVFIVDDNFY
                     GVGEKHAQGAREVLQAIIKRGKKHHWFSQTSFNMGSKPDDLRLAYKAGCRAMLVGIES
                     FNEENLKSWGKALNRSILHDYQKHADGFHKAGVAILGGFVIGTDNDTVDTAV"
     gene            complement(1137..1409)
                     /locus_tag="PLK08_03450"
     CDS             complement(1137..1409)
                     /locus_tag="PLK08_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPS52588.1"
                     /translation="MNIYVGNLSYRVNDDELRSFFEQFGEVSSAKVVMDRETGRSRGF
                     GFVEMPNDEEGKDAIAKSNEKELQGRALKVNEARPREEKPRRNFNN"
     gene            1763..2164
                     /locus_tag="PLK08_03455"
     CDS             1763..2164
                     /locus_tag="PLK08_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPS52589.1"
                     /translation="MNYLKGRLVKLMIDFWEEDFRRITHALAVMRYSEIIMPDFCDYD
                     HDVILAASLLHDVGIKPSEAQLGYNNGKTQEQFGPAVATELLESISFPEEKIKKVAEI
                     ISNHHSPDRYDYVELKILKLADRIVNAADPD"
     gene            complement(2295..4475)
                     /locus_tag="PLK08_03460"
     CDS             complement(2295..4475)
                     /locus_tag="PLK08_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPS52590.1"
                     /translation="MSDCRKAAIEAISKIHTFNKCVDYTVTNVKEVYCEHVFSESVQR
                     ERLPKAVFKSLQQIIKAGKEINSEIADAVALAMRDWAIEHGATHYTHWFQPMTGTTAE
                     KHDSFITPTGDGEILAQFSGKDLVRGEPDASSFPSGGLRATFEARGYTAWDPSSPAFI
                     IENPNGATLVIPTMFISWTGEALDKKTPLLRSMEVLDKAALRLLRLFGNTTASRVICT
                     LGAEQEYFLVDKHFTMNRPDIINTGRTLFGARPPKGQELEDHYFGAINERVLAFMAEV
                     EYELLRLGVPIKTRHNEVAPAQFELAPTFETANLAIDHQMLIMEMLRKVAGRHGMMCL
                     LHEKPFAGINGSGKHNNWSISTDDGENLLEPGHTPALNAQFLTFCAAVCRAIHKFAPI
                     LRVTAASAANDHRLGANEAPPAIISVFLGKQLTEVFEQLETGKAAQDKEVEPIKLGIS
                     MLPKLPRDAGDRNRTSPFAFTGNKFEFRAVGSSQNPSAANTVLNTIVAESLDFMSDSI
                     EKQMKKGKDVNQAVQAILPAVIKEFKPVLFDGDGYSEAWHKEAKKRGLPNLKNTVEAL
                     PEILKKENVDLFKKYGVYSERELHSRYDILLENYVKTITIEADTCLMMAQSMILPTAI
                     KYQTAIAESIGKLKAAGATSKTQLGMLKSITADIDALKMAIDKLTDNMSNIPHKNLLV
                     TSKYMRDIIFVGMSEVRAVADRLEVVIDDNLWPLPTYREMLFIR"
     gene            complement(4698..6863)
                     /locus_tag="PLK08_03465"
     CDS             complement(4698..6863)
                     /locus_tag="PLK08_03465"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPS52591.1"
                     /translation="MMCIVVCSFCGMTSADGGNQEIASKAGITYLLSRISPSGACLDE
                     PKDHTWPDYGVRTATVISTLLSSNLNPSDTPELERAIKWLMNFSPKTTAALAARIIAL
                     SRVKQKDALSAAAEDVKKLDDYAYADGGFGPAAANGQPRQAGDDCNNQHTSLATQAFD
                     AARQKNIDVPIKTWQKLNAYWLAQQLPSGGFSWTKYRGSSGELANPYGSMTAAGIVGA
                     YMSYSALRIANLSAAEKLDTANDLATTAGIAWLAGHMSFDFNPDKGAEWYYQWLEQLG
                     LMCRLTGYRQINGCWVQDRINVKLISRQNADGSWGDGERILQTCRAINALAKTGGPVF
                     LNKIRFPGRWNIHFDDARNIVSWLEYTYEQPMNWQVVNIDRPTDWDILGRICYLSGTG
                     RVDFSDSQVDTLRNFIERGGVLLSESANGDGEFSLSIQRLYQKLFPTIRKLTLPADTP
                     LLNLKFRTASPSPLVGLSNGIRLLAVHIPRGISWQLEKNQFMRDRSTFEFFGNLLFYL
                     YPNPARDYKPLKSNLPNGRCIVKTSAILPRIKFAGNWNPEPLAMQFFNYFYTNKTGRR
                     LITGYNFVAFKDLDASKFKAAVLSGTGEFPLLTEKEILTIQKYLIDGGTIIADAAGSD
                     KTFARTFVQNFMAKLGKPKNFNPPAEPGRLDVSYEMSAIYVDSRPAVMFSPTDIQAGL
                     VGYSLNGLESWAGNADNIMLNLIEKSGIAATKTSLKSKK"
     gene            complement(7018..>7976)
                     /locus_tag="PLK08_03470"
     CDS             complement(7018..>7976)
                     /locus_tag="PLK08_03470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HPS52592.1"
                     /translation="ILGCLVIIFSIMLNFCLLAVVAGEDISDLNKTVLQGGSDEQVVA
                     VYCVNGVIDDKAAEDFSRFYRTIGKSGKIRAVVLRIDSPGGGIAASDEIYKMVTELKA
                     AGKKVVVSMGGVAASGGYYISAPANYIFAEPATITGSIGVIMQLLNLEGTMNKLGMKA
                     ITITSSNAALWKDQGSPFRQMSKRERENFVAMLNSMQSRFESIVTAGRPKLVTKKETY
                     KDEIIVDGKTRQVTKTDTAPFNGQIYGTAKAINIGLVDEQGYLPDAWKKAASLAGLKN
                     AKVVQFTKRTGFLFEMADGLSSFRKASSEVSAPRFLMQWGAD"
BASE COUNT         1828 a         1940 c         2049 g         2159 t
ORIGIN      
        1 acagctgtgt ctacggtatc attatctgtg ccgattacaa aaccgccgag tattgcgacg
       61 ccggctttat ggaagccgtc ggcatgtttc tgatagtcgt gtaaaatgct gcggttgagg
      121 gctttgcccc agcttttgag attttcttca ttgaaagatt caatgccgac caacatggcg
      181 cggcagccag ccttgtatgc cagccgcaga tcgtccggtt tgctgcccat gttaaaactg
      241 gtctggctga accagtgatg ctttttgccg cgcttgatga ttgcctgcaa cacttcacgg
      301 gcgccctgcg cgtgcttttc gcctacgccg taaaaattgt cgtcgactat gaaaacgaac
      361 ggttttttta tggtattcca ttccgcgatg atatcgtcga ccggccgtcg ccgtatctgt
      421 ttgccgttga aacgtgttac gctgcagaat ttgcagccta tcgggcagcc gcgtgctgtc
      481 tgaatgcagg cgacgtcata tttgccgttt attgcctgca tggactggtc tgccgtgccc
      541 aagccgttcg ttagttcgat caaatagcct tcatagcgag gtttcaaaca gccagcctgt
      601 gcgtcggcga tgatatcgtg ccagatagcg tcacattcgc cgactgcgac cgtgctgaaa
      661 tattgactag cctcatccgg acaggctgac gcatgagggc cgcccatcat ggctggcaga
      721 ttatgttgac gacaaaatac agctaattcg tatgctcgcg gcgcgctgct ggtatatgaa
      781 gtaattccga caaagtcgta gccgcggctg agcagaagtt cttccgtgtc attcgtattg
      841 aaaatttcgt cgatgatttc aacctgatgt tcagcaggcg tcaacttggc gagtacctgt
      901 aggccgagcg gcgggatttt cgtatttatc gaatggtatc ctgaagtatt gcgcgtagcc
      961 ggattaacca gtgcgatttt cattttattt ggactcgcaa tgtgaaagtg ttgcgtaaag
     1021 aacgtaaggg gagttgactt ctgaaaacct gtttcaatat acatccacct tacgtttgct
     1081 actgcgaacg taaaaaccag aagagacaga ttttcagaat caccagggcg actgttttag
     1141 ttgttgaagt tgcgtcgcgg tttttcttca cgcgggcggg cttcattgac tttcagggca
     1201 cggccttgca attctttttc attggacttg gcgatggcgt ctttgccttc ttcgtcgttg
     1261 ggcatttcca cgaagccaaa accacggctg cggcccgttt cgcggtccat tacaaccttg
     1321 gcggaagaaa cttcaccgaa ctgctcaaag aaactgcgga gttcatcgtc attgacacga
     1381 tacgaaagat tgccaacata aatattcacg gtcatagcta ccttttttgg ctggcctgca
     1441 cgacagttct ctgaaaaggc agccaaacga ccgtttcagg aacacacacg ggattcgccg
     1501 ggcaggcaaa tccaacgtat tattagacca aaattcgtga aaaatacaaa agaaataatc
     1561 ttctgcggca aaaatatctt tatatgcttt gctggttaaa gaagttacag gattcacctg
     1621 actcgcgcaa ggcggggaga atcatgagat gcgactccaa aaagaaaata atttttagaa
     1681 ccgctctatt gtgaatagcg gtataatacc ggtttcccac aaaaaatcgc tgcaagagtc
     1741 attacatgtt tggagaggcg aaatgaatta tctcaaaggc cggctggtaa aattgatgat
     1801 tgatttctgg gaagaagatt ttcgcagaat aactcacgcc ttggccgtca tgcgatatag
     1861 cgaaatcatt atgcccgatt tctgcgatta cgaccacgac gtaattcttg ccgcgtctct
     1921 gttgcacgac gtcggcataa aaccatccga ggcacagttg ggctataata acggaaaaac
     1981 acaggaacaa ttcggcccag ccgttgcgac tgaactactt gaaagcatca gctttccgga
     2041 agaaaaaatc aaaaaagtag cggaaataat cagcaatcat cattcgccgg accgctatga
     2101 ctacgttgaa ctcaaaatct taaaacttgc cgaccgcatt gtaaacgcag ccgaccctga
     2161 ttgattttta ttcaaaccag ggtatgctct gaataaaaaa agacccagcc ataatgactg
     2221 ggtctttttt tatttctgcg attcactcaa ataaaaacac ttgccgattt tttgtcggaa
     2281 aagttattgg aaaattatcg gataaaaagc atttctcgat aggtcggcag cggccagaga
     2341 ttgtcgtcta ttacgacttc cagacggtct gcgactgctc tgacctcgct catgccgacg
     2401 aatatgatat cacgcatgta ttttgacgtg acaagcagat ttttgtgcgg tatattcgac
     2461 atattgtcgg ttagcttgtc aatagccatt ttcaacgcat cgatgtcggc ggttattgat
     2521 ttaagcatgc cgagttgcgt ttttgatgtt gcgccggcgg ctttgagctt gccgattgat
     2581 tcggcaatag cagtctgata cttgatagcc gtgggcagaa tcatcgactg agccatcatc
     2641 agacatgtat ccgcttcgat ggttatggtc ttgacgtagt tttctagaag tatgtcgtag
     2701 cggctgtgca attcgcgttc ggaatagacg ccgtatttct taaagaggtc tacgttttcc
     2761 ttcttcagta tttctggcag tgcttcgacg gtattcttga gattcggcag gccgcgtttt
     2821 ttggcttcct tgtgccacgc ttcactataa ccgtcgccgt cgaagaggac gggcttgaat
     2881 tcttttatga cagccggaag aattgcctga acagcctggt tgacgtcttt gcccttcttc
     2941 atctgttttt cgatggagtc ggacatgaaa tcgagagatt ccgccacgat ggtattgaga
     3001 accgtattgg ccgccgatgg gttctgactt gagccgacgg ctcggaactc gaatttgttg
     3061 ccggtaaatg caaacggact ggtgcggtta cggtcgcccg catcgcgcgg cagcttgggc
     3121 agcatggaga tgccgagttt aatcggttcg acttccttat cctgggcggc tttgccggtt
     3181 tcaagctgtt caaaaacttc cgtaagctgt ttgccgagaa atacggaaat aatcgccggc
     3241 ggagcttcgt tggcgccgag acgatgatca tttgcagccg acgcggctgt tactcgcaga
     3301 atcggggcga atttatggat tgcacggcag acggcagcac agaatgtaag aaactgtgca
     3361 ttcaatgccg gcgtatggcc aggttcaagg agattttccc catcgtccgt gcttattgac
     3421 cagttgttat gtttacccga gccgttaatg cctgcgaacg gtttttcatg cagcaggcac
     3481 atcatgccat gccggccggc aacttttcgg agcatttcca taataagcat ctgatggtca
     3541 atggcgaggt tggcggtctc gaacgtcggg gccaattcga actgtgcggg ggcaacttcg
     3601 ttgtggcggg ttttaatcgg cacgccgagc cgcagaagtt catattctac ctcagccatg
     3661 aatgccagaa cgcgttcatt aattgcgccg aagtagtggt cttcaagttc ctggcctttt
     3721 ggcggccggg cgccgaaaag cgttctgccg gtgttgatga tgtcgggacg gttcattgtg
     3781 aaatgtttgt ccacgaggaa gtattcctgt tcggcgccga gagtgcagat tacgcggctg
     3841 gccgtcgtat tgccgaaaag cctcagcaga cgcaaggcag ccttgtcgag gacttccatc
     3901 gaacgcagca gcggagtttt tttgtcgaga gcttcgccgg tccagcttat gaacatggtc
     3961 ggaatcacca gcgtcgcgcc gttggggttt tctattatga atgccgggct ggaaggatcc
     4021 catgcggtat agccgcgcgc ttcaaacgta gcacgcaaac cgccggacgg gaaactggag
     4081 gcgtcaggct cgccgcggac aagatccttg ccgctgaact gtgccaggat ttcgccgtcg
     4141 ccggtcggcg taatgaagct gtcatgtttt tccgccgtcg tgccggtcat cggctgaaac
     4201 cagtgcgtat aatgcgtcgc gccgtgttcg attgcccaat cgcgcatcgc aagggctacg
     4261 gcgtcggcga tttcgctgtt gatttccttg ccggctttta taatctgctg cagagatttg
     4321 aaaaccgcct tcggcagccg ttcacgttga acggattcgc tgaacacgtg ttcgcaataa
     4381 acctctttta cattagtaac ggtatagtcc acacacttat tgaaggtgtg gattttgctt
     4441 atcgcctcaa tagcggcttt gcgacaatcg ctcatgttcc tgattccttt ctgaattatc
     4501 agcccggttt tgacgaaacg cgggcacaaa gactctctgc gaaactgcta caatctccgt
     4561 gccagatttg tcaccggaaa ttatcaacac gccttaattt tttataacta ttgtatctgc
     4621 agtgcattag tcaaaaatat caaaacctac aaaaaagttc aacattggaa ctttatctta
     4681 gaatagctac ataattgtta ttttttgctt tttagagacg tttttgtagc ggctatgcct
     4741 gatttttcaa tgagattcag cattatattg tcggcattgc cagcccacga ctcaagcccg
     4801 tttaacgagt atcctacaag gccagcctga atatcagtcg gcgaaaacat gaccgctggc
     4861 cggctatcga cgtaaattgc agacatttca tatgatacgt caagcctgcc gggttctgcc
     4921 ggcggattga agtttttggg tttcccgagt tttgccataa aattttggac aaaagtgcgg
     4981 gcaaatgtct tatcgctgcc ggcggcatcg gcgattatcg tgccgccgtc tatcaggtat
     5041 ttttggattg taagtatttc cttttcagtc agaagcggaa attctcctgt gccggataaa
     5101 accgccgcct tgaactttga tgcgtccaaa tctttgaaag ctacaaaatt gtagcccgta
     5161 atcagacgtc tgccggtctt gttcgtataa aaataattga aaaactgcat ggcgagaggt
     5221 tccggattcc aattgccggc gaattttatg cgaggcagaa tggcggacgt ttttacgatg
     5281 catcttccgt tgggcaggtt gcttttgaga ggtttgtagt cacgcgccgg attcggatag
     5341 agataaaaca gcaagttccc gaaaaattca aatgtcgacc tgtcgcgcat gaactggttt
     5401 ttttcaagct gccacgatat gccgcgtgga atatgtaccg ccagcagccg gatgccattg
     5461 gacaggccta ccagcggcga cggcgacgct gttctgaatt tcagattcag cagcggcgtg
     5521 tcagccggca gggtaagttt acggattgtc ggaaaaagtt tctggtacag cctttggata
     5581 ctgagagaaa attcaccgtc gccgttggca gattcgctga gaagtacacc gccgcgctcg
     5641 atgaaatttc gcaacgtgtc cacctggctg tcgctgaaat caacccggcc cgtgcctgac
     5701 agatagcaga ttctgcccaa aatatcccaa tcagtcggcc ggtcaatatt gactacctgc
     5761 cagttcatcg gctgctcata ggtgtattcc agccaactga cgatatttct ggcgtcgtcg
     5821 aaatggatgt tccatctgcc gggaaagcgt atcttgttca aaaatacagg gccgcctgtt
     5881 ttagccaggg catttattgc gcggcaggtc tgcaaaattc tttcgccgtc gccccagctg
     5941 ccgtcggcgt tctggcggct gatgagtttg acgtttatcc tgtcctgaac ccagcagccg
     6001 ttaatctgtc ggtaaccagt caggcggcac atcagcccaa gctgttccag ccattgataa
     6061 taccattccg cacccttgtc tggattgaag tcgaaactca tatggccggc gagccacgcg
     6121 atgccggctg tcgtagccag atcgttggcg gtatcgagtt tttcggctgc gctcaggttg
     6181 gcgattcgca gtgcggaata gctcatgtat gcgccgacga tgccggctgc ggtcatcgag
     6241 ccgtaaggat tggccagttc ccctgagctg ccgcggtatt tcgtccatga aaaaccgccc
     6301 gacggcaact gctgggccag ccagtaagcg ttgagttttt gccaggtttt tatgggtacg
     6361 tcgatgtttt tctgtctggc ggcgtcgaag gcttgcgtgg cgaggctggt gtgctgattg
     6421 ttgcagtcgt cgccggcctg tcgcggctgg ccgtttgctg ctgccggccc gaaaccgccg
     6481 tcggcatacg catagtcatc caattttttc acatcttccg ccgctgcgga taaggcgtct
     6541 ttctgtttga ccctgctcag ggctattatg cgggcggcca gcgcggcggt cgtttttgga
     6601 ctgaaattca ttagccattt tattgcccgc tcaagttcgg gagtgtcgga aggatttaga
     6661 tttgacgata aaagcgtgct gatgacggtg gccgttcgta cgccgtaatc cggccaggtg
     6721 tggtctttgg gttcgtcgag acatgcgccg gacggagaga tgcgggagag caggtatgtt
     6781 atgccggctt tgctggcgat ttcctgattg ccgccgtcgg ctgacgtcat gccgcaaaaa
     6841 gaacagacga cgatacacat cataaaaaat atcgtcgtcc gttttgtact gtaatttgtg
     6901 ttcatccgcg gaaaattcgt gttgaaggac aaatcgcaac cggcagcagt cagttatgaa
     6961 aaaaccaaaa atgctctgtg gcagcaggct gcctctgcct gttggctggg tgaaaaatca
     7021 atccgctccc cactgcataa ggaatctcgg ggcggagact tctgacgagg cttttctgaa
     7081 actggaaagg ccgtcagcca tttcgaataa aaatcccgta cgtttggtga attgtacgac
     7141 tttcgcattt ttcaggccag ccagcgaagc ggcttttttc catgcgtccg gcagataacc
     7201 ctgttcgtct acgaggccga tattgatagc cttggctgtg ccgtatatct ggccgttgaa
     7261 cggcgccgtg tcagttttgg taacctgccg cgttttgccg tcgacgatta tttcatcctt
     7321 atatgtctct tttttcgtga cgagttttgg ccggccggct gtgactatcg attcaaacct
     7381 gctctgcatg ctgttgagca tggcgacgaa attttcacgt tcgcgtttcg acatttgcct
     7441 gaacgggctg ccctggtctt tccagagagc cgcgttggaa ctggtaatcg taattgcctt
     7501 catgccgagc ttgttcattg ttccttcaag gttcagcagt tgcatgatta cgccgatgct
     7561 gcctgttatc gtggctggtt cggcaaagat gtaattcgcc ggcgcggata tgtaataccc
     7621 gccgcttgct gccacgccgc ccatcgatac gacgactttt ttaccggcag ccttcagttc
     7681 cgtaaccatt ttatatattt catcgctggc tgcgattccg ccgcctggag aatctattcg
     7741 cagaaccaca gccctgattt tacctgattt tccgattgta cgataaaagc gggaaaaatc
     7801 ttcggcggct ttatcgtcaa ttaccccgtt gacgcaatag accgctacga cctgttcgtc
     7861 gctgccgccc tgcaacacgg ttttattaag gtcagaaata tcttcgccag ccactacagc
     7921 caacaaacag aaattaagca ttatggaaaa aatgattacg aggcatccga gtatat
//
LOCUS       DAONFI010000071         7917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_10604_length_7917_cov_4.138896, whole genome shotgun sequence.
ACCESSION   DAONFI010000071 DAONFI010000000
VERSION     DAONFI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10604_length_7917_cov_4.138896"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..367)
                     /locus_tag="PLK08_03475"
     CDS             complement(35..367)
                     /locus_tag="PLK08_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPS52593.1"
                     /translation="MVRDANKMSMSPQWLEQAAECLRVMAHPARLRMIELLNRQPCSV
                     KEIAAYCSLPHNQACEHLRLMKSHGLLDAERDGQTVYYRLVAPQAMGLLHCLQQNCPE
                     WNTASKKK"
     gene            637..1122
                     /locus_tag="PLK08_03480"
     CDS             637..1122
                     /locus_tag="PLK08_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPS52594.1"
                     /translation="MGTKKYNSKFPYAFRNDWHEALVRTWHTGQLLRAIAVNFFADKP
                     VSDTQFNLMGGLYYSDKPPSQQELADRLVVHKSAISTLIDRMEQAGLVRRRSHSGDRR
                     RFELVLTEKGEKIFKNLEEEYLELVRSIMKRISNRDITALNESLEKLRLAVNAYLINK
                     E"
     gene            1127..1504
                     /locus_tag="PLK08_03485"
     CDS             1127..1504
                     /locus_tag="PLK08_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52595.1"
                     /translation="MFTLRTFTNILRVIAKIYAGVMALLIVMFLVGENGLVNPFVLPL
                     STAIKFFCIWAICIGCMAGWKWQGAGGIIVIAGVLVFHIVEKRFWLAGLFPFFDVAGI
                     LYLISWLLWKKVDFKGRSTHSCG"
     gene            complement(1564..2301)
                     /gene="hisA"
                     /locus_tag="PLK08_03490"
     CDS             complement(1564..2301)
                     /gene="hisA"
                     /locus_tag="PLK08_03490"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPS52596.1"
                     /translation="MELLPAIDLRNGCVVRLEKGDYAKQTTYEVSPLATAEKYVSAGA
                     KWIHIVDLDAALTGELTNTQIIRDVCKFAAANDMKVENGGGIRDTRRVKMLLDMGVRR
                     VVVGSTAMKNFPWFAELMNDASIPNECIALGLDARDGKVAAQGWTQQLDITAVELARR
                     VSGSGLGAIVYTDIARDGMLQGVNIESTADVIAATNVPVIASGGVAGLDDIRKCLAIG
                     CGGVILGKALYEGKINLADAVKLVKEK"
     gene            complement(2352..2972)
                     /gene="hisH"
                     /locus_tag="PLK08_03495"
     CDS             complement(2352..2972)
                     /gene="hisH"
                     /locus_tag="PLK08_03495"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPS52597.1"
                     /translation="MIALIDYGMGNLRSVEKALERCGGQVKIVTTPSGILSADKVVLP
                     GVGAMRDAMNRINSTGIADAVRETITAGRPLLGICLGLQMLLDRSFEYGEFKGLGIIP
                     GDVVRFDFSGTSNSGLKIPHMGWNSINFNADCPLLAGIDQGAYVYFVHSYYVCPKDQS
                     VTAATCNYGVDFTAAIWRDNLFATQFHPEKSQAVGLKILENFVKKI"
     gene            complement(3071..3760)
                     /locus_tag="PLK08_03500"
     CDS             complement(3071..3760)
                     /locus_tag="PLK08_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPS52598.1"
                     /translation="MKKWTVSCMAVALCAFGVIGCNEREKGQIAALTERVDSLQAQNK
                     NLNDRNVELEKQVDQYKMEIEDKNMQLAQKDKMIKDLEGNLGKTKNEEVTVIKNPAGW
                     EVGMNGAKLTIGSDILFNSGKASLTSSGKSKLAQAYRDIQNNYGGWSVRVFGYTDSDP
                     IRKSKWKDNLELSGERAMSVTRYLTSQGISPDLVETIGMGATHFVASNSSRSGKAQNR
                     RVEIVVVKPTK"
     gene            complement(3867..4181)
                     /locus_tag="PLK08_03505"
     CDS             complement(3867..4181)
                     /locus_tag="PLK08_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52599.1"
                     /translation="MTLKLFTAYVKILVSLIFFLLVIMLFFSNAGNTCSNLQFFTKTY
                     NDVSLAKLMLTCCAVGAIAVFMAWQLFTGGSTLHRYRKTHPASTADQPCRNEDENLYE
                     NK"
     gene            complement(4198..5391)
                     /locus_tag="PLK08_03510"
     CDS             complement(4198..5391)
                     /locus_tag="PLK08_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPS52600.1"
                     /translation="MSCIFIFGLCMGSFMNVVVWRLPRGGSIVFPSSHCPVCGYKIRW
                     FDNIPLISYLILLRCRCRRCKVFISPRYPVVELTTALLMVTLYVCFILLHMKPGFDDL
                     TAGKARLTFKEFQQYFPYIFSYCILTWALLSCSLTDIATFTLPIGVCWFASIVGTLAA
                     TFWPNEDLMPRISHPLAAASCAAGVGLIISVILQHMGFLIPSFIDADYRLSNTEAADG
                     NVVAATAGCGISPRKEILREILFLTPAVTMAIAAYYLVSKTVIGDLWDGVYALDLHQA
                     GVFMDGFSASLFGYLVGGMWIWGIRIFGTLLFNKEAMGMGDVHLLAAVGAVCGWITPS
                     LTFFVAPVFGLIYAIMILLRKHQKELPYGPWLSLAAYTVILFQKYILAFLMLENMPLH
                     FLATK"
     gene            complement(5602..6171)
                     /locus_tag="PLK08_03515"
     CDS             complement(5602..6171)
                     /locus_tag="PLK08_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HPS52601.1"
                     /translation="MNLKGKKIAIMLDEQYQEMEVWYPYYRFTEAGATVDLVGPDAEK
                     SYKSKLGYPCQSTCSAGSVVAKMYDAVIVPGGWCPDFMRRDERMVHFIQDCAKEGIIL
                     AAICHGGWMLCCTDALRGKTATSFMAIRHDMMNAGADWIDEECVIDGNLITARKPDDL
                     PAFCTAIAQQLENFNPNRKAILRIADFTM"
     gene            6309..7463
                     /locus_tag="PLK08_03520"
     CDS             6309..7463
                     /locus_tag="PLK08_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52602.1"
                     /translation="MESQKSFSADLEFLNENSSPVVLTAPDGGGRVVVSPGMQGRVMT
                     STADGKSSYGWINYSLIESGRRREHINAFGGEDRFWLGPEGGQYSIFFKAGEPMDINH
                     WQTPEPIDWGSWDVVSTNKERIEMVKGFVLDNAAGCKFSLRTRRVIKVFSRADIAKTL
                     GVEISDGVNYVGFESLNAITNAGNRFEKEYGLLSIWTLGMFKASSKAVVVVPFKADAA
                     AGAHDLINAGYFGRVSDDRLNVDVRKGVAFFRGDGKSRGKIGVSPKYAKEILGSWTPE
                     LNTLTIVMYSLPANAEKLDYVNSTWEIQKEPYSGDAVNSYNDGPAEAGGLQLGQFYEL
                     ETSSPAMVLGPSDKQMHTHRTFHFTGKKKHLNEMAQQLLGMSMDEIEKAL"
     gene            7627..>7917
                     /locus_tag="PLK08_03525"
     CDS             7627..>7917
                     /locus_tag="PLK08_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPS52603.1"
                     /translation="MSENDDNNNAEKPLDFIRQMVADECAGGRWGGKVITRFPPEPNG
                     YLHIGHAKAICIDFGVAEEFGGECHMRFDDTNPTKEDDEYVQSILEDIHWLGF"
BASE COUNT         1884 a         1912 c         2062 g         2059 t
ORIGIN      
        1 ttttttccgt tttgccacct tttttttaaa actcctattt tttctttgac gcagtgttcc
       61 attcgggaca gttttgttgc aggcagtgca gcaggcccat cgcctgcggg gcgaccagcc
      121 ggtaataaac agtctggccg tcgcgttccg cgtcgagcag gccgtgggac ttcatcagcc
      181 gcagatgttc gcaagcctga ttgtgcggca gggaacagta tgcggcaatc tcttttacac
      241 tgcacggctg acggttgagc agttctatca tccgcagccg ggcagggtgc gccatgaccc
      301 gcagacattc cgccgcctgc tccagccact gcggcgacat cgacatttta ttagcatcac
      361 gaaccatatg gccattacct cgaaattttc aaacttcgtt acaaaatgca atattctgtc
      421 aggccggata ttgaaatgcc tgacttccgg tcttattgat atattatata tcgagatatc
      481 ccgatatttc aagtattatt gataaaattt aaaaccgctc catgtgaaga tgtgtatagc
      541 ctggttttct atatgcgata cgtttgataa atgcagggaa atggaagaat gataaaaata
      601 gttgacatat aaactgtttg catataaact atatttatgg gtacaaaaaa atataattcg
      661 aagtttccgt atgctttccg aaacgattgg cacgaagcgc tggtgcgtac atggcatacc
      721 ggccagcttc tgcgtgcgat agccgtgaat ttttttgccg acaagcctgt atcggatacg
      781 caattcaacc tgatgggcgg attgtattat tctgataagc cgccgagtca acaggagctt
      841 gccgacagac ttgtagtcca taaatctgcg atttcaacct tgatagacag gatggaacaa
      901 gcaggtttgg tcagacgccg cagccattcc ggcgacaggc gaaggtttga actggttttg
      961 actgaaaaag gtgaaaaaat tttcaagaat ctggaagaag aatatcttga gctggtacgt
     1021 tcaataatga aacgtatttc aaaccgtgac attacagctc tgaacgagtc tcttgaaaaa
     1081 ctgcgattgg cggtaaatgc atatcttatc aataaggagt agaatcatgt ttacgcttcg
     1141 aacgtttaca aacattttga gagtgatagc caagatttat gccggtgtga tggcattgtt
     1201 gatagtgatg tttcttgtcg gggaaaacgg tcttgtgaat ccttttgttt tgccattatc
     1261 cacggctata aaattttttt gcatatgggc catatgtatt ggttgtatgg ctggctggaa
     1321 gtggcagggg gcaggtggga ttatagtgat tgcaggtgtt ctggtttttc atatagttga
     1381 aaaacgtttt tggcttgccg ggttgtttcc attttttgat gttgccggaa ttttgtatct
     1441 gatatcgtgg ctgttgtgga aaaaagtcga ttttaaaggc cgctctacac attcttgcgg
     1501 atgaaatcga aaaaggctaa agaagtttgt atggattaat tggcggtttt gtgcaatctg
     1561 attttatttt tcttttacta gtttgactgc gtcggcgagg ttgattttgc cttcgtaaag
     1621 ggctttgccg agaattacgc cgccgcagcc gattgcgaga catttgcgga tgtcgtccaa
     1681 acccgctacg ccgccggatg caatcaccgg cacgttggtg gcggcgatga cgtcggcggt
     1741 cgattctatg ttcacgccct gtaacatgcc gtcccgcgcg atgtcggtat agactatcgc
     1801 tcccagtccg ctgccgctaa cgcgcctggc cagctccaca gccgttatgt caagctgttg
     1861 cgtccagcct tgcgccgcga ccttgccgtc gcgcgcgtcc agacccaatg cgatacattc
     1921 gttcggtatc gacgcatcgt tcatcaattc cgcgaaccag gggaaattct tcatggcggt
     1981 tgagccgact acgactctgc gtacgcccat gtcgagcagc atctttacgc gccgcgtatc
     2041 gcgtatgccg ccgccgtttt caaccttcat atcattggcg gctgcgaatt tacatacgtc
     2101 gcgtattatc tgcgtgttgg taagttcgcc ggtgagtgcg gcgtcgaggt ctactatgtg
     2161 tatccacttt gcgccggcgg agacgtattt ttcggcggtg gccagcgggg atacttcgta
     2221 ggtggtctgc tttgcgtagt cgcctttttc aagccgtaca acgcaaccgt tacgcaaatc
     2281 tatcgcgggc agaagttcca taaaaatatt cctcgattta ttaccggcag ccggcagtcg
     2341 tggggggact gtcaaatttt tttcacgaaa ttctcaagta ttttcaagcc gaccgcctga
     2401 cttttttccg gatgaaactg cgtggcgaag agattgtccc gccatattgc cgcagtgaag
     2461 tcgactccgt agttacaggt ggctgccgtt accgactggt ctttgggaca gacgtagtag
     2521 ctgtgcacga aatagacgta cgcgccctgg tcaatgcctg ccagcagcgg gcagtcggcg
     2581 ttgaaattga ttgaattcca gcccatgtgc ggaattttga gaccggagtt cgacgtaccg
     2641 ctgaaatcga aacggacgac gtcgccgggg attattccca agcctttaaa ttcgccgtat
     2701 tcaaacgaac ggtccaaaag catctgaagg ccgaggcaga tacccagcaa tggccgaccg
     2761 gcggtaattg tttcacgcac ggcgtcggca atgccggtgg agtttatacg attcatcgcg
     2821 tcgcgcatcg cgcctacgcc gggcaacacg actttgtcgg cagatagaat cccggacggg
     2881 gttgtcacta ttttaacctg tccgccgcaa cgctccagcg ccttttccac gcttcgcaaa
     2941 ttgcccatgc cgtaatctat caacgctatc atgccagtca aacatccttt cgcagtatgc
     3001 aaataacaag gccctgccga tttgctcgac agggccttga agtttaactc attttcgctt
     3061 tcaggttaag ttattttgtc ggttttacga ccacaatttc aactcgcctg ttttgtgcct
     3121 tgcctgagcg ggaactgttc gacgccacga aatgcgttgc gcccatgccg atggtttcaa
     3181 ccaggtcagg actgatgccc tggctggtga gatatcgcgt tacgctcatc gcgcgttcgc
     3241 cggaaagttc aagattgtct ttccacttgc tcttgcgaat cgggtcgctg tcggtataac
     3301 cgaaaacgcg aacgctccag ccgccgtaat tgttctgtat atcacgatat gcctgcgcca
     3361 gcttggattt gccgcttgat gtgagcgatg ccttgccgga gttgaacaga atatcgctgc
     3421 cgatggtcag tttcgcaccg ttcatcccga cttcccagcc ggcgggattt tttatgacgg
     3481 tcacttcttc atttttggtt ttgccgaggt tgccttcgag gtcttttatc atcttgtcct
     3541 tctgggcaag ctgcatattt ttgtcctcga tttccatctt gtattgatcg acctgttttt
     3601 caagttctac attgcggtcg ttgagatttt tgttttgcgc ctgcagtgaa tcgacccgct
     3661 cggtcagggc tgcgatttgt cctttttcgc gctcattaca gccgattacg ccgaaagcac
     3721 aaagagcaac agccatacaa gatactgtcc acttcttcat tttcgtctcc ttggtgacac
     3781 aaatccttct taaaaaagcc attaacgttc atcgtcaatc aacgtgtggt ggattataca
     3841 acaatcgctg attatgcgac agaaatttat ttattttcat ataaattttc atcctcattc
     3901 cggcatggct ggtcggcagt gcttgccgga tgagtttttc gatatctgtg gagtgtactg
     3961 ccgcctgtga aaagctgcca tgccatgaat acggctattg cgcctacggc gcagcaggtc
     4021 aacatgagct tcgccaggct tacgtcgttg taggttttgg tgaaaaactg caaatttgaa
     4081 cacgtattgc ccgcgttcga gaagaaaagc ataataacca gcaggaaaaa tatcagcgag
     4141 accaatattt tgacgtacgc agtgaacagt ttcagtgtca tatctaaccc tttcatttca
     4201 ttttgtggca aggaaatgca gcggcatatt ttccagcata aggaacgcca gtatgtattt
     4261 ctggaacagt ataacagtat atgccgccag actcaaccac ggcccatacg gaagttcttt
     4321 ctgatgtttt cgcaaaagta tcattattgc gtaaatcagg ccgaatacag gggctacgaa
     4381 aaaagtcagc gacggcgtaa tccagccgca cactgcgcct acggcggcta agaggtgtac
     4441 gtcgcccatg cccatcgcct ccttgttgaa cagcagtgtg ccgaaaatgc gtatgcccca
     4501 tatccacatt ccgccgacga gatacccaaa aaggctggcg gaaaagccgt ccatgaaaac
     4561 ccctgcctga tgcaggtcaa gagcgtaaac gccgtcccat aaatcgccta taactgtttt
     4621 gctgacaaga taatatgctg cgatagccat tgtgacggcg ggcgtaagga agagtatttc
     4681 cctcaaaatt tcctttcgcg ggcttatgcc gcagccggcc gtcgcagcta ccacgttgcc
     4741 gtcggcagct tctgtattgc tcagccgata atccgcatct atgaaacttg gtatgagaaa
     4801 tcccatatgt tgcaggatta cggagattat aaggccgacg ccggctgcgc agctggctgc
     4861 tgcgagcgga tgtgaaatgc gcggcatgag gtcttcattg ggccagaatg ttgcggctaa
     4921 tgtcccgacg atggatgcga accagcatac gccgattggc agcgtgaacg tggcgatatc
     4981 cgtcagcgag cacgacagca atgcccacgt cagtatgcag tacgaaaaaa tatagggaaa
     5041 atattgttga aattccttaa aagtgagcct ggctttgccg gcggtaaggt catcaaagcc
     5101 gggtttcatg tgcagcagta tgaaacacac atagagcgtg accatcagca gggctgtggt
     5161 gagttccact acggggtatc gcggcgagat gaacaccttg cagcggcggc agcggcaacg
     5221 cagcagaatg agataactta tcagcgggat attgtcgaac cagcgtattt tatagccgca
     5281 gaccgggcag tggctggatg ggaaaacaat actgccgccg cgcggcagac gccagacgac
     5341 gacgttcatg aagctgccca tgcacaaacc gaatatgaat atgcaactca tccagtagat
     5401 atgcatgaac actataggcg tctccgtttt tgccggcttt tccgaccgcg gaaaagtttt
     5461 tcaggcggtt ttgatgacaa tacattatca gcatattcgc cggaattcaa gtcgctttta
     5521 gggcgcatca aacttgacct gccataattt ctgctggcgg cagccggtat ttaacggcgc
     5581 ggcgaacaga cggcggtggc gtcacatcgt gaaatcggcg attcgcagta ttgccttgcg
     5641 gttcggattg aaattttcga gctgctgggc gatggcggtg cagaacgcgg gcaggtcgtc
     5701 gggttttctg gcggtgatga gattgccgtc gattacgcat tcctcgtcaa tccagtctgc
     5761 gccggcgttc atcatgtcgt gccgaatggc catgaagctg gtcgccgttt tgccgcgaag
     5821 tgcgtccgtg cagcagagca tccagccgcc gtggcagatg gctgccagga ttatgccctc
     5881 tttggcacag tcctggatga agtgcaccat gcgttcgtcg cgtcgcatga agtccggaca
     5941 ccagccgccg gggactatga ccgcgtcgta catttttgca acaacgctgc cagctgagca
     6001 ggttgactgg cagggatagc ccagtttgga cttatagctc ttttccgcat cggggccgac
     6061 gaggtcgact gtggcgccgg cttccgtgaa acggtagtaa ggataccaca cttccatttc
     6121 ctgatattgt tcgtcgagca taatcgctat ttttttccct tttagattca tctgccgtct
     6181 cctgttttta ctgtgggatt aattatatcc tgtttgacta tgagacttga ttttactatc
     6241 ctgtcgtggt taaaacaagc gtctcaatat gcaaattgag attcggaaga atattcggaa
     6301 ttactgacat ggaatcgcag aaaagttttt cagccgattt ggagtttttg aacgagaact
     6361 ccagccctgt cgttctgact gcgccggacg gcgggggccg ggttgtcgta tcgcccggca
     6421 tgcaaggccg cgttatgacc tcgaccgccg acggaaagag cagctacggc tggataaatt
     6481 acagccttat cgaatcgggc cgccgccgtg aacatatcaa tgctttcggc ggagaagaca
     6541 gattctggct cggcccggaa ggcggacaat acagcatatt cttcaaagcc ggcgagccga
     6601 tggatatcaa ccactggcag acgcccgagc cgatagactg gggcagttgg gacgtggttt
     6661 cgacgaataa agaacgtatc gagatggtga aaggttttgt gctggataac gcagccggct
     6721 gcaagttcag cctgcggacg cgacgcgtaa tcaaggtttt cagccgagcc gacatcgcca
     6781 aaacgctcgg cgtggaaatt tctgacggcg tgaattatgt gggctttgaa agcctcaacg
     6841 ccatcactaa cgccggcaac cgtttcgaga aagaatacgg cctgttgtcg atatggacgc
     6901 tgggcatgtt taaggcatct tccaaggctg tggtggttgt gccgttcaag gctgacgctg
     6961 cggctggtgc gcatgatttg ataaacgccg gctacttcgg ccgtgtttca gacgacaggc
     7021 tcaatgtaga tgtccggaaa ggcgtagctt ttttccgcgg cgacggcaaa agccgcggca
     7081 aaatcggcgt atccccgaaa tacgcaaaag aaatcctggg cagctggacg ccggaactca
     7141 acacgctgac tatagtgatg tattcgctgc cggctaacgc tgaaaagctc gattacgtca
     7201 actccacgtg ggagattcag aaagaaccct acagtggcga cgcagtcaat tcgtacaacg
     7261 acgggccggc tgaggctggc gggctgcaat taggccaatt ttacgaactg gaaacctcat
     7321 cgcctgccat ggtgctcggc ccgtccgaca agcagatgca tacgcatcga acattccatt
     7381 tcaccggaaa gaaaaaacat ctgaatgaaa tggctcaaca attgctcgga atgagcatgg
     7441 acgaaattga gaaggcgttg taaatcagat tgattttaat atgtccttcg taattctacg
     7501 gttttaaaac cgtaatatga aagtatccgc tgtgatgtgg acaggcgaat gcctgatgaa
     7561 tatgatgcta cgggttttaa ccggtagaga ttcactttat gattcaaaat attcggagtt
     7621 tcaaaaatgt ctgaaaatga cgacaataat aatgcggaaa aaccgctgga tttcatacgt
     7681 cagatggttg ccgacgaatg tgccggcggc cgttggggcg gcaaggtcat aacgcgtttt
     7741 ccgcccgagc cgaacggtta tcttcacatc ggccacgcga aggctatctg catagacttc
     7801 ggcgtggcgg aggaattcgg cggcgaatgt cacatgcgtt tcgacgacac caatcctacg
     7861 aaggaagacg atgaatacgt gcagtcgatc cttgaggaca ttcactggct gggtttc
//
LOCUS       DAONFI010000072         7680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_11066_length_7680_cov_4.536000, whole genome shotgun sequence.
ACCESSION   DAONFI010000072 DAONFI010000000
VERSION     DAONFI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11066_length_7680_cov_4.536000"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLK08_03530"
     CDS             <1..246
                     /locus_tag="PLK08_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52604.1"
                     /translation="YRWQRELGEQTALEAYQRQLLPFRACKRVYKFDRYMDVLDETLP
                     AMQKYVLGIDNDKLQIWMNLEQDTTNILGEAIGGGTK"
     gene            324..1256
                     /locus_tag="PLK08_03535"
     CDS             324..1256
                     /locus_tag="PLK08_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPS52605.1"
                     /translation="MKRHFGIIILALLVLVILLVYTVAFVVDQTRDIVIITQFGKVNR
                     VIDGRTDPGLHFKWPYPIENMIRYDSRDQILVSSYKQFSIGEKYSIMASVFCTWRITD
                     PIKFNSSRQTVKNAQDSLTALLNTEMGGQFGNVTMDELVNTDPKAMKIEPICKRIRDN
                     VNATAMSDYGIEITDLGVNRIGLPQSVSQSVITAMSEERSKEISKYQAEGQAIAEAIV
                     SRASSAKEKILAFAQRKAGDIRTQGETKAAESYAKFNADPEFSMFLRSVESLRASLKD
                     RSVFLLDAQTLPILDWMRTEPTLESFRNGKENNK"
     gene            1289..2680
                     /locus_tag="PLK08_03540"
     CDS             1289..2680
                     /locus_tag="PLK08_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPS52606.1"
                     /translation="MEHIHSSHCHHDRDDDDANQPIDPAMQSLADALKVSFRVLSIGM
                     LLFVLLFLATGIGSVEPQEQGIVKVFGKVTRTVPKGLVYNWPFPIGEIQKVSTMEKKL
                     EIDSFWMFETPQDKLKPLSARSRNNQGLRPGYDGYLITGDRNIIHIKFACIYKVADPL
                     TYVSVLADPEQALREVLCDAAVEAAATRTADSIQYNHELFLNQVQELAQKKIDAVMGF
                     DSKGGGHGIVINNILLPQPDAKTWPLGAFDAYEKAQRAKSEKQTAINKAVATAKETLI
                     NSVGSINYIELVGEPWEDVQKCSEQPAYNLIALYGQIDNAADNAAVVCAEKPTAANKA
                     ALEKIRTDRRRIWDTINVILTRATIGGEASKIIAQAESEKTAIIENAQRRAKRFDELH
                     VQYEKAPQLFLNKMWANVYDELMSSPTVEKWYLSPGKMGSILKINSDPAVKQAIRDYK
                     LKQEKERKTREQQ"
     gene            2677..3888
                     /locus_tag="PLK08_03545"
     CDS             2677..3888
                     /locus_tag="PLK08_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS52607.1"
                     /translation="MTATQNVVHIRGLTKIFRDFWLREKVTAVSELNLDIRSREVFGL
                     LGPNGSGKSTTLKMILGLLFPTRGTISVFGKHPTNVNVKANIGFLPEESYLYPFLDAR
                     ETLDFYGQLFHLPHRQRMRRIDMLLDMVGLSAAAYRRVGEYSKGMQRRIGLAQALIND
                     PELLILDEPTSGLDPIGTKQFKDLIRMLSQRGKTVILSSHLLGDVEDVCDRVSILYGG
                     KLRREGNVEDMLSQKTKTQIITDELTQQDLEKIMQVLDGKSVEISHPKTRLETLFLQI
                     VDEARRQKLKTGGATAGGKLAEFLLGNENDTGSAQGKKLLDELVDDTKSRVLSQEQAQ
                     TTAESEENRKQQARRHDMLESLVKKDTAEPNAQQPAAGADETTPQTHDNSSADRDIID
                     GLLGKDEDSQA"
     gene            3885..5549
                     /locus_tag="PLK08_03550"
     CDS             3885..5549
                     /locus_tag="PLK08_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52608.1"
                     /translation="MKNIFTVAAQTFSQCLRMKIAVSCIMLLVIALAAVGFMGQGDGT
                     LAGRIRTCMNYSLGLTGMLLSVVTIFAIVSVVTEDVRQKFVMVLAVKPLARYQYMLGR
                     WLGVVMLNAVLLAIAGLSTYVLLQHLRNLPAVNSADRQAVETEIFTARQELAPVPPDI
                     QMLFDKKIEQLQKEGRYNSILQEFLIQSGDNADQAKLKMQKELEKEILAWIEFVPANG
                     QKLYQFRGLALKDQAVDGNGRVEIAEIKDGLFRINAPTRLISKMFYNGPVQINGIRGR
                     IIRMEQTFFDVVFEPRDMQRSELQNLKKNAEVAIIVEPTIQFRYKVKIAGKSSSASEL
                     FRAIAFLDSNKKPNGVLIMGEGPVNTVTTINAPAIGLGEGDFYVVYANVPKPAQSVMS
                     VSAPAIPLIIPMNDVRVLYRVGGFEANFFRALLLILFQLAFLAALGVFCSSFLAFPVA
                     SLACFALLGCGLLVNWVSESLSWGKTAGGFDFVNFMGSLVVEILRALMPNLGSTSPAE
                     KLVDGKVLSWVAVGVTFIKVVIVRTFIYLAMGCLIYNKRELAKVQV"
     gene            5549..7525
                     /locus_tag="PLK08_03555"
     CDS             5549..7525
                     /locus_tag="PLK08_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52609.1"
                     /translation="MNRSRFIQIGCLLLAVILLVCSGLLLGPILKKSEKADLLTDDNL
                     ASMRRNPEIAMLMTMPGGLRVLFVNYLWIRSQDAHQEGRHYDAYQLAELICKLQPYHP
                     GVWAFQAWNMAWNISVTTFTPDQRWQWVYNGVKLLRDQGISYNPRSLELYKELAWIFS
                     SKIAGMLDDEHNAYKARWAMIMQNLLGAPPVDLNSASSLEEDTQKVIDAFAAIAAAPL
                     DRDKARQGKEMIQADKLATVLTDPQCQTYAERLKKIGIEIGQPLLDAYNSYSLDFAVQ
                     SIKIVTPKLADSRAKEISAIINDVTAKTSREKLLSFVRAQLLWNEFRMDPEYMLKLMK
                     RYDAPLDWRNAMSHGLYWSDYGMDLCQPGKIAGMVALNNARNVLNSLKALTWTGNISL
                     RYNPENPDYPVYMESADLRYVKATQNQYMQYINMRLNEKEDKTFDKNILQAGHVNYLV
                     SAIRMLVADGRESAAQGYYEFLKDDYKKQGPQWEYTDVRQFVLSNFREEMESQLRHEV
                     VMQILTYSLKQAYVYRGLIGGSRGQELYKKQMTFSKNIYDIYQTKAVVRMKLLPFEVV
                     ASNVLARLIVRPGLFGLSLDVDQRSNIFRSMSNEQELAQYTYLMTKPILMSLCKDAGV
                     DFDTAFPMPAGTEELRQKLMRKMNDENPEKASKK"
BASE COUNT         2130 a         1798 c         1973 g         1779 t
ORIGIN      
        1 taccgctggc agcgggaact cggcgaacag accgcgctgg aagcatatca gcgacagtta
       61 ctgccgttcc gcgcctgtaa acgggtatat aaatttgacc gctatatgga tgtgctggac
      121 gaaactctgc ctgcaatgca gaaatacgtt ctcggaatag ataacgataa attgcagata
      181 tggatgaatc tcgaacagga tacgacaaat attctgggcg aagcaatagg cggcggaacg
      241 aaataaaaac tgtgtcgtgc cgcaaaaccg tgtaaaaaat tctaaaaatg gaagatggaa
      301 aataaaacag ctggagcagg aaaatgaaaa gacatttcgg aatcataata ctggcacttc
      361 tggtattggt aatattgctg gtatatacgg tggcgttcgt tgtcgaccag acgcgggata
      421 tcgtgataat cacgcagttc gggaaagtca atcgcgtcat cgacggcaga accgatcctg
      481 gcctgcattt caagtggccg tatccaatcg aaaacatgat acgctacgac agccgcgacc
      541 agatactggt ttcgtcgtat aaacagttca gcatcggcga aaaatacagc attatggcca
      601 gcgtgttctg cacgtggcgc attactgacc cgataaagtt caacagttcg cggcagactg
      661 taaaaaatgc gcaggattct cttaccgcgc tgctcaatac ggaaatgggc ggccagtttg
      721 gcaatgttac tatggatgaa cttgtcaaca ccgacccaaa agccatgaag atagaaccga
      781 tttgcaaacg tatccgcgat aacgtcaatg ccactgcgat gtccgattac ggtatcgaga
      841 ttaccgacct gggcgtcaat cgcataggcc tgccgcagag tgtttcccaa tccgtcataa
      901 ccgcaatgag cgaggaacgt tccaaggaaa tcagcaagta tcaggcagaa ggccaggcta
      961 ttgcggaggc tatcgtttcg cgggcatcgt cggctaagga gaaaattctg gcattcgccc
     1021 agcgcaaagc cggcgacatc cgtacgcaag gtgaaacaaa agccgctgaa tcctacgcca
     1081 aattcaatgc cgatcctgaa ttcagcatgt tccttcgcag cgtcgaaagc ctccgcgcca
     1141 gcctaaaaga ccgcagcgtt ttcctgctgg atgcccagac gctgccgata cttgactgga
     1201 tgagaaccga acccactctc gaaagcttca ggaacgggaa agaaaacaac aaataaacaa
     1261 taaatgtaaa acgaacagga ctgataaaat ggaacatatt cacagttctc attgccatca
     1321 cgaccgagac gatgacgatg cgaatcagcc gatagatccg gccatgcaaa gccttgccga
     1381 cgcactcaag gtaagtttcc gcgtgctttc catcggcatg ctgctgttcg tgctgctgtt
     1441 tcttgcgacg ggcatcggca gcgtcgagcc gcaggagcag ggcatagtaa aagttttcgg
     1501 caaagttacc cgtaccgtgc cgaagggcct ggtgtataac tggccgtttc caatcggcga
     1561 aatccaaaaa gtctccacta tggagaaaaa acttgagata gattctttct ggatgttcga
     1621 aacgccgcag gacaaactca agccgctatc tgcacggagt cgcaataatc agggccttcg
     1681 gcctggatat gacggctatc tcataaccgg cgaccggaac atcatccata taaaattcgc
     1741 atgcatatac aaagtggctg acccgctaac ctatgtttcc gtattggctg acccggaaca
     1801 ggcgctgcgt gaagtgcttt gcgatgcagc cgtcgaagcc gccgctacgc gcacagccga
     1861 cagtattcaa tataatcacg aactgttcct gaaccaggtc caggaactcg cgcagaaaaa
     1921 aatcgatgcc gtcatgggat ttgattcaaa aggcggcgga catgggatag tgataaacaa
     1981 catactgctg ccgcaacctg atgccaaaac ctggccgctc ggtgcgtttg acgcctatga
     2041 aaaagcccag cgcgcaaaaa gcgaaaaaca gaccgctatc aataaagccg tcgccaccgc
     2101 aaaagaaacg cttatcaatt ccgtcggttc gataaactat attgaactgg tcggcgagcc
     2161 gtgggaagac gttcaaaaat gttccgaaca acccgcatat aatctcattg ccctttacgg
     2221 ccaaattgac aacgccgcgg ataatgccgc agtcgtctgc gccgaaaagc cgactgccgc
     2281 aaacaaggct gcgctggaaa aaatccgaac cgaccgtcgc aggatatggg atacgataaa
     2341 tgtgatactc acccgcgcga cgataggcgg cgaagccagt aaaatcattg cccaggcaga
     2401 gagcgaaaaa acggctatca tagaaaacgc tcaacgccgc gcaaaacgtt ttgacgagct
     2461 ccatgtgcaa tatgagaaag cgcctcagct tttcctcaac aaaatgtggg ctaacgttta
     2521 tgacgaactg atgagttcgc cgacggtcga aaaatggtac ttatcgcccg gcaaaatggg
     2581 atcgattttg aaaatcaatt ccgatcccgc cgtaaagcag gcaatacgcg attacaaact
     2641 caaacaggaa aaagagcgta aaaccaggga acagcaatga cagccactca aaatgtagtt
     2701 catatccgtg ggttgacaaa aatattccgc gacttctggt tgcgtgaaaa agtgaccgcc
     2761 gtcagcgagc tcaacctgga cattcgttcg cgcgaggttt tcgggctgct cggccctaac
     2821 gggtcgggca aaagtaccac gctgaaaatg atattggggc tgctttttcc tacacgcggt
     2881 acgataagcg tgtttggaaa acatcccacg aatgtaaacg tcaaagccaa tattggattc
     2941 cttcccgaag aatcgtattt atatccgttt ctggatgcac gggagacgtt ggatttttac
     3001 ggccagcttt tccatctgcc acacaggcaa cgcatgagac gtatcgacat gctgctggac
     3061 atggtgggtc tttccgcggc tgcataccgt cgcgtgggcg aatattcaaa aggcatgcaa
     3121 cgacgcatcg ggttggcgca ggcgttgata aacgacccgg aactgctcat actcgacgaa
     3181 cctaccagcg ggcttgaccc aatcggcacg aagcagttta aggatttgat acgtatgctt
     3241 tctcagcgag gcaaaactgt gatactttcc agtcatctgc tgggcgatgt cgaagatgtc
     3301 tgtgacaggg tgagcattct ttacggcgga aaactgcgac gcgaaggcaa tgttgaagac
     3361 atgctctcgc aaaaaacaaa aacgcagata attaccgacg aacttactca acaggatttg
     3421 gaaaaaatca tgcaggtgct tgatggcaaa tccgtagaaa tcagccatcc gaaaaccagg
     3481 cttgaaaccc tgtttttaca gattgtcgac gaggcacgca gacagaaact taaaactgga
     3541 ggtgcgacgg ctggcggcaa acttgcggag tttcttctcg gcaatgaaaa tgacaccggc
     3601 tctgcgcagg gcaaaaaact tctcgatgaa cttgtagatg atacgaaatc gcgggttttg
     3661 tcgcaggaac aggcccaaac gactgctgaa tcggaagaaa accggaagca gcaggccaga
     3721 cggcacgaca tgcttgaaag tcttgtaaaa aaagataccg ccgaaccaaa cgctcaacag
     3781 cctgccgccg gcgcagatga aacgacaccg cagactcacg ataattcaag cgcagaccgc
     3841 gatattattg acggattgtt gggtaaagat gaggatagtc aggcatgaaa aatatcttca
     3901 cagtggctgc ccagacattt tcgcagtgtt tgcggatgaa gatagccgtc tcttgcatca
     3961 tgttgctggt catagcactg gctgcggttg gattcatggg ccagggggac ggtacgttag
     4021 ccggccgaat ccgcacatgc atgaactatt cgcttggcct gactggaatg ttgctgtcag
     4081 tcgtgacaat atttgcaatc gtcagcgtcg ttacggaaga cgttcgccaa aaattcgtca
     4141 tggtgttagc cgtcaagcct ctcgcgagat atcagtatat gctgggcaga tggttgggcg
     4201 tggtgatgct caatgcggta ttgttggcga tagccggtct ttctacgtat gtcctgttgc
     4261 agcatcttcg caatcttcct gcggtgaatt ctgccgacag gcaggctgtt gagacggaaa
     4321 tttttacggc cagacaggaa cttgcgcctg ttccgccgga tattcagatg ttgttcgata
     4381 aaaagataga gcagcttcag aaagaaggcc gttataacag catattacag gaatttttaa
     4441 tacagtcagg cgacaatgcc gaccaggcga aactgaaaat gcagaaagag ctggaaaagg
     4501 aaattttggc ctggattgaa tttgttccag ccaatggcca gaagctgtat cagttcagag
     4561 gcctggcgct caaagaccag gctgtcgatg gtaatggcag ggttgaaatc gctgaaatta
     4621 aagacggcct gtttcgtata aatgcaccga cgcgtctgat tagcaaaatg ttttataacg
     4681 ggccggtaca gattaacggc atcaggggca gaatcataag gatggagcag actttcttcg
     4741 acgtggtttt cgagccgcgc gacatgcagc gcagcgaatt gcagaatctc aaaaaaaacg
     4801 ccgaagtcgc gataattgtc gaacctacga ttcagttccg ttataaagtt aaaattgccg
     4861 gcaaaagttc atctgcgagc gaactgtttc gtgcgatagc gtttctcgac tcgaataaaa
     4921 aacccaatgg cgttcttatc atgggcgaag ggccggtgaa taccgtcacg acgataaatg
     4981 cgccggcgat aggccttggc gaaggcgatt tttacgttgt ttacgccaat gtgccaaaac
     5041 cggcacaatc agttatgagc gtatcggcgc cggctattcc gttgataatt ccgatgaatg
     5101 acgtcagggt gctttatcgc gtgggcgggt tcgaagccaa ttttttcagg gcgctgctgc
     5161 tgatattatt tcagttggcg ttcctggcgg cgttgggcgt gttctgttct agttttttag
     5221 cctttccggt ggcgtccctt gcctgttttg ccctgcttgg ctgcggtttg ctggtaaact
     5281 gggtaagcga gtccctctcg tggggcaaaa ccgccggcgg atttgatttt gtaaatttca
     5341 tgggcagcct tgtcgttgaa attcttcgcg ccctcatgcc gaacctgggc agcacgtctc
     5401 ctgctgaaaa gctggtcgac ggcaaggttt tatcgtgggt cgcagtcggc gtgacgttca
     5461 taaaagtagt gattgtaaga acgttcattt atcttgctat gggttgtctg atatataata
     5521 aacgcgaatt ggcgaaggtt caggtctgat gaatcgttcg agatttatac aaatcggttg
     5581 tctgttgctg gcggtcatct tattggtctg ctcaggtttg ctgcttggcc cgatattgaa
     5641 aaagtccgaa aaagcggatt tgcttacgga cgacaatctg gcctcgatga ggcgcaatcc
     5701 cgaaattgcg atgttgatga caatgccggg cggcttgcgt gtgctgtttg tgaattacct
     5761 gtggatacgc tcgcaggatg cacatcagga agggcgtcac tacgacgcct atcagcttgc
     5821 cgaacttata tgcaagttgc agccatatca tccaggcgtg tgggcttttc aagcctggaa
     5881 catggcctgg aacatatcgg taacaacatt tacgcccgac cagcgatggc agtgggtcta
     5941 taacggcgtc aaactgctgc gcgaccaggg catttcatat aatccccgtt cccttgagtt
     6001 gtacaaggag cttgcatgga tattttcatc aaaaatcgcc ggcatgctcg acgatgaaca
     6061 taatgcgtat aaagcacgct gggccatgat tatgcaaaat cttctcggcg caccgccggt
     6121 ggatttgaac agtgcttcgt cgcttgaaga agatacgcaa aaagtgatag atgcgtttgc
     6181 agctatcgcc gccgctccgc ttgaccgcga caaggcgcgg cagggcaagg agatgataca
     6241 ggcagataaa ctcgcaacag ttcttaccga cccgcaatgt caaacctatg ccgaacggct
     6301 gaaaaaaatc ggtattgaaa tcggtcagcc gctgctggat gcgtacaaca gctacagcct
     6361 cgattttgcc gtccagagca taaagatagt tacgccgaaa ctcgctgaca gccgcgcaaa
     6421 ggaaatatcc gcaattatta atgacgtcac tgccaaaacg tcgcgagaga aactgctttc
     6481 gttcgttcgc gcgcaattac tctggaatga attccgcatg gacccggaat acatgttgaa
     6541 gctcatgaag cgttatgatg cgccgcttga ttggcgcaac gccatgagtc acggattata
     6601 ctggtccgat tatggaatgg acttgtgcca gcctggaaaa attgccggta tggttgcact
     6661 taacaacgcg cgcaatgtgc tcaactccct caaagcgctg acatggacgg gcaatatttc
     6721 tctccggtac aatccggaaa atccggatta tccggtttat atggaatctg ccgacttgcg
     6781 atacgtgaaa gctacgcaga atcagtacat gcagtatata aatatgcggc tgaacgaaaa
     6841 ggaagacaaa accttcgaca aaaatatcct gcaagccggc catgtcaatt acctcgtttc
     6901 cgccattcgc atgctcgttg ccgacggcag ggagtctgcc gcccagggtt attacgagtt
     6961 tttgaaagat gattacaaaa aacagggccc gcaatgggaa tatacggatg tgcgtcaatt
     7021 tgttttgagc aattttcgtg aagagatgga atctcagctg aggcacgaag ttgttatgca
     7081 gattttgacg tattcgctca agcaggcgta tgtttatcgc gggttgatag gcggcagccg
     7141 tggccaggaa ctttataaaa aacagatgac attttcgaaa aatatttatg acatatatca
     7201 gacaaaagcg gttgtaagga tgaagttgtt gccgtttgag gttgttgcgt ccaatgtgct
     7261 ggccaggctt attgtgcggc ctgggctgtt cggccttagt cttgacgtcg accagcgaag
     7321 taatattttc cgttccatgt cgaatgaaca ggaattggca caatatacat atcttatgac
     7381 aaaaccgatt cttatgtcgc tatgcaaaga tgccggagtg gattttgata ctgcttttcc
     7441 catgccggcg ggtactgaag aattaagaca aaaactcatg cgaaaaatga acgacgaaaa
     7501 ccctgaaaaa gcatcgaaaa agtgaagttc tgttgtaatt ccccaaaaat gtgatgttaa
     7561 agcggttctc caaagttttt tctgttgtct tgcgatcaga cactgaaata tgctggcaat
     7621 cgagaattac tagaacctat cccaaaaaca tgggcgtcgc cgaaatcgag caattttgcg
//
LOCUS       DAONFI010000073         7655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_11113_length_7655_cov_4.683684, whole genome shotgun sequence.
ACCESSION   DAONFI010000073 DAONFI010000000
VERSION     DAONFI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11113_length_7655_cov_4.683684"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLK08_03560"
     CDS             <1..801
                     /locus_tag="PLK08_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPS52610.1"
                     /translation="SLGLLLPYVIRKYSSGAQSGRLTRSGNALEVLVLFVRDQIAAPS
                     LGAKAPAFLPLLLTLFVFIFGMNLIGLTPLHPITVWVSEFLSTQITGHRDVYPIGMSP
                     TSILTVCLSLALMTLTCILACGLWKAARRSRLPLPAALVLSPLIWFKNLAPHVPGATG
                     KILCIPLAILELVGLVAKCFSLMVRLAANMIAGHVMLAVLMMFAIESLQSFISTAMAS
                     YHFLYIAPICIMACVLMYIMELLVAVLQAYIFTFLTALFLGLYVEPSH"
     gene            1047..1298
                     /gene="atpE"
                     /locus_tag="PLK08_03565"
     CDS             1047..1298
                     /gene="atpE"
                     /locus_tag="PLK08_03565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPS52611.1"
                     /translation="MKRSAISIGTGFAAGGACVGAGLAAIGGGYAIARVGGTCIESMA
                     RQPEAAGSMFMPMIVTAAMIEGAMLFAIVVSLMAVLYSF"
     gene            1316..1915
                     /gene="atpF"
                     /locus_tag="PLK08_03570"
     CDS             1316..1915
                     /gene="atpF"
                     /locus_tag="PLK08_03570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPS52612.1"
                     /translation="MTHGRISIRSCAAACVCTLGCSVSYASAAGGGGLYFGDLGQALA
                     SVIAFVILLLILKKFAWKPVLEQLDKREKDIQSRLKDAHDKEEKASELEEDYRHRMQA
                     VNAEAEQIRNEIRRQAEEQKQQIIAATQAQAHQMIQRAGADIETAKAEAVHQLQSQTA
                     RLVAEIAQHVLGRNLSSDEHARLIEEASDYIADKVGDDR"
     gene            1912..2523
                     /gene="atpH"
                     /locus_tag="PLK08_03575"
     CDS             1912..2523
                     /gene="atpH"
                     /locus_tag="PLK08_03575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPS52613.1"
                     /translation="MKQLDVYISDNSVQPQVCRLAEIYAQALIESLDGKVSPADAADE
                     LASLDKLIRSLPGGEQLMTCPTVSLNRRQKIVDEVGRFFSQPVANLLAVLAKNSRLEL
                     IGMIADACEKVVVRKAGEVEMVVRSAVKLDAATKNKIAGQLGDAIGAKCIIENVVDPR
                     IIGGLILQKGDTIFDASIAGRLQRMAQAVADAKQVSNTQEVDR"
     gene            2520..4205
                     /gene="atpA"
                     /locus_tag="PLK08_03580"
     CDS             2520..4205
                     /gene="atpA"
                     /locus_tag="PLK08_03580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPS52614.1"
                     /translation="MKFRSDEIASVLREEITNYKADLDVSHVGRVLEVGDGIARIYGL
                     SGAMSAEMLKFESGATGQVFNLEESSIGAVIYTDVDKVRAGDIVKGTGQLLDVPVGDY
                     MLGRVIDPLGNPLDGGLPIATIRRRPVEVPAPGISARQPVERPLQTGIKAVDSMTPIG
                     RGQRELIIGDRKTGKTAIAIDTIINQKDTGVICVYVAIGQKESTVAGVVETLRQYGAM
                     DHTIVISASSSAAAPLQYIAPYAGCSIAEYFMYQGRHTLVVYDDLTKQAAAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLADKYVVAPKNTPDAELEKITPVYTGPIEKK
                     NAQAAIDGMKDESLELRLLPQSGGSMTALPICETQEGEVSAYIPTNLISITDGQIYLE
                     PALFFAGVRPAINVGISVSRVGYKAATKAMKKVAASLRLDLAAYRELESFAQLGMELD
                     PASQRQLDRGQRMVQLLTQPQSSPMSVKDQVISIALGTSGKLDDIDARYVSTFEKYFL
                     TYLRESEADFYKEFCENPAELDSSLKRLAFLAEEYKSTRWEQDVKNARKAGEV"
     gene            4251..5150
                     /gene="atpG"
                     /locus_tag="PLK08_03585"
     CDS             4251..5150
                     /gene="atpG"
                     /locus_tag="PLK08_03585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPS52615.1"
                     /translation="MAKARKILARTKAIKNIHTVIRTMEMVSTARFKKAFNRTSSARP
                     YIEGMASLVEDILMRCNPEKIYHPLFRRPEKLRNANVILITSDRGLCGGYNSALCKMA
                     EGRLREFTDGDVTTKLYVVGKKGLTHMHRDRFEVMHDYIGLDPESQGWQWASSLGDEF
                     MSQFIAGQLDCVEVVYSRLVGMGGHQPTCRALLPLAFQLDEDKDVKKLTMSQRVEYEF
                     IPSAGDMLDHLLPMTVRLELYQWLMEAAVTEQIARMAAMRSANENATEMIRKLTVQYN
                     RTRQAQITTELAEILGGAVALEG"
     gene            complement(5158..6558)
                     /gene="asnS"
                     /locus_tag="PLK08_03590"
     CDS             complement(5158..6558)
                     /gene="asnS"
                     /locus_tag="PLK08_03590"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPS52616.1"
                     /translation="MISVQRISQILNASQHKPGDTVTVGGWVRTVRHSKGGFSFISLN
                     DGSCFDSLQVVADSSLGNYGDVEKLTTGSSLLASGVLVASQGRGQTVELQARSLTVYG
                     YADAAAYPLQPKRHSFEFLRTQAHLRPRTSTFSAVARVRNRICSAIHEFFQQRGFIYV
                     HTPLITTSDCEGAGEMFAVTTLDMDNPPRNEAGQVDYSQDFFARPTFLTVSGQLAVEP
                     YACSLGNVYTFGPTFRAENSNTPRHLAEFWMVEPEMAFCDLEGDAAVAESFLKHIFGT
                     VLADCDADMKFFDERINPNLLKTLEHITASNFERLTYTNAVEVLQAANRQWDFNPYWG
                     CDLQTEHERYLTEEYLKKPVIVTDYPKSLKPFYMKCNKDDKTVAAMDVLVPGVGEIIG
                     GSQREDNLDAIESRMAEMHMKKEDYWWYLDLRRFGSVPHAGFGLGLERTVQFCSGMSN
                     IRDVIPYPRTPRNAEF"
     gene            6660..7583
                     /locus_tag="PLK08_03595"
     CDS             6660..7583
                     /locus_tag="PLK08_03595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPS52617.1"
                     /translation="MHVLPEKKQTMFWLIIGTEITGIVVMIGLLAYKKFIADHFFTSH
                     ERKPAVSVSYTSRPTHSARPLFAQTRVESDTPVKIESVPAAAAVACVQSQPAEEAVPA
                     KLSSASPDAAEPSTPEISDLNEQGDSSSWQIFSEENKSDKNSAAAGPPLPKVIKNPKI
                     IVIKSRQILEVYDGGKLLRTYQASTGSNPGDKEKSGDRKTPEGEFYICVKNPKSRFVL
                     SLGLSYPEYDDARRGLRTGLISQNEYSHIISELNMKRQPPWNTKLGGEVMIHGVRQGG
                     RSTAGCIALENNDIRELYPRIPIGTKVIVKP"
BASE COUNT         1841 a         1955 c         2126 g         1733 t
ORIGIN      
        1 agcctgggtc tgctgctgcc gtacgtcatt cgcaaatatt catccggcgc acagtctggc
       61 cgacttactc gaagcggcaa cgccctggaa gtgctggttc tctttgtacg cgaccagatt
      121 gccgcgccgt cgctaggcgc caaagccccc gcatttctgc cgctgctgct gactctgttc
      181 gtcttcatat tcggcatgaa ccttatcggc cttacgccgc tgcatcccat taccgtctgg
      241 gtgagcgaat ttctttcgac gcagataacc ggccaccgcg atgtgtatcc tataggcatg
      301 tcgccgacga gcatactgac ggtctgtctg tctctggcgt tgatgacgct tacctgcatt
      361 ctggcgtgcg gactctggaa ggcggcgcgc cgcagcaggc tgccgctgcc ggcggcactt
      421 gtgctttcgc cgctgatatg gttcaaaaat ctcgctccgc acgtgccggg ggcgacgggt
      481 aaaatcctct gcattccgct tgcgatactt gagctggtag ggcttgtggc gaaatgtttt
      541 tcgttgatgg tgcgtctggc ggctaacatg atagccggcc acgtcatgct tgccgtgctg
      601 atgatgtttg ccatcgaatc gttgcagtca tttatttcaa cggctatggc gagctatcat
      661 tttctgtaca ttgcgccgat ttgcataatg gcgtgcgtgc tgatgtatat aatggaactg
      721 ctggtggcag ttttgcaggc gtatattttc acgtttctga cggcactgtt tctggggttg
      781 tatgttgagc cttcacatta ataaccgcaa ctacaacttt ttaacaatta gaaaataaat
      841 ttagaaaaat tttaaaaaaa agaaaaaaag aaaagtagga aatttttgat gaatttttga
      901 taaatacaca ggagacagcg atgaaaaaac ttgcaatgtt cggattggcg atgatggtac
      961 tcggcggcat atgcacggtt gcgatggcag ccgatgagcc gaagacggat gaagcttctc
     1021 ttcaggctca gcaggacgac gagcatatca agcgcagcgc gatatcaata ggcacaggtt
     1081 ttgccgccgg cggtgcgtgt gtcggcgccg gccttgccgc cataggcggc ggctacgcca
     1141 ttgcccgcgt cggcggaacg tgcatcgaat ccatggcccg gcagccggaa gcagccggca
     1201 gcatgttcat gcccatgatt gtcacggcag ccatgattga aggtgcaatg ctgtttgcga
     1261 ttgtcgtttc attgatggcg gtcttgtaca gcttctgacg gcggaggttt ttaccatgac
     1321 acacggcagg atttcaatcc gctcatgcgc agccgcctgc gtctgtacgt taggatgctc
     1381 agtttcgtat gcatcggcgg cgggcggggg cggattatac ttcggcgacc tcggccaggc
     1441 gctggcgagt gtgattgcgt tcgtcatcct gctgctgata ttgaaaaaat ttgcatggaa
     1501 gcctgttctt gaacagctcg acaaacggga gaaagacata cagtcccgtc tgaaggatgc
     1561 ccatgacaag gaagagaagg cctccgaact tgaagaggat tacaggcaca gaatgcaagc
     1621 cgtcaacgct gaagccgagc agattcgcaa tgaaatccgg cgtcaggcgg aagaacagaa
     1681 gcagcagata atcgctgcga cgcaggcgca ggcgcaccag atgattcagc gtgccggcgc
     1741 cgacatagag acggccaaag cagaagccgt ccatcaactt cagtcccaga cggcgcggct
     1801 ggttgcggaa atcgcccaac acgttctggg ccggaatctc tcaagcgacg aacatgcaag
     1861 attgattgaa gaagccagcg attacatcgc cgacaaagtc ggagacgacc gttgaaacag
     1921 ttggacgtat atatttccga taattccgtt cagccgcagg tttgccggct ggcggaaatt
     1981 tacgcacagg cgctgataga atcgctcgac ggtaaagtgt cgccggcgga tgcagccgat
     2041 gaactcgcct cgctggataa acttatccgt tcattgccag gcggcgaaca gttgatgaca
     2101 tgtcccacgg tgtcactgaa caggcggcag aaaattgttg atgaagtcgg ccgttttttt
     2161 tcgcagccgg ttgcgaatct gctggctgtg ctggcgaaaa acagccggct ggaacttatt
     2221 ggcatgatag cggatgcctg tgagaaagtt gtagtcagaa aagccggcga agttgaaatg
     2281 gttgtccgct cggcggtgaa gctcgatgcc gctacgaaaa ataaaatcgc cggccagctc
     2341 ggcgatgcga ttggcgcaaa atgcataatc gaaaatgtcg tagacccgcg aataatcggc
     2401 ggccttattt tgcagaaggg cgacacgatt ttcgatgcgt cgatagccgg ccgtcttcag
     2461 cggatggctc aggcggttgc agacgcgaaa caggtttcca atacgcaaga ggttgacaga
     2521 tgaagttccg ttcagatgag atagcaagcg ttcttcgcga ggagataacg aattacaagg
     2581 ctgacctcga cgtttctcat gtcggccgcg tactggaagt cggcgacggc attgcccgca
     2641 tttacggcct ttccggcgcg atgtcagccg agatgctcaa attcgaatcc ggcgcaaccg
     2701 gccaggtatt caaccttgag gaaagttcca tcggcgcggt catttacacc gacgttgaca
     2761 aggttcgagc cggcgatatc gtgaaaggca caggtcaact gctcgatgtg ccggtaggtg
     2821 attacatgct cggccgggta atcgacccgc tcggcaatcc tctggacggc ggtttgccca
     2881 ttgccacgat tcgccgccgg ccagtcgaag tgcctgcgcc tggcatcagt gcacgacagc
     2941 ccgtcgaacg gccgttgcaa accggcataa aagccgtcga ttccatgacg cccatcggcc
     3001 gcggccaacg cgaactcatc atcggcgacc gcaaaacagg taaaaccgcc atcgcaatcg
     3061 atacgataat aaatcagaag gacaccggcg tcatatgcgt gtatgtcgct atcggccaga
     3121 aggaatccac cgttgccggc gtagtcgaaa cgctgcggca gtacggtgcg atggatcaca
     3181 ccatcgttat ttccgcgtcg agttcggcgg cggctccgct tcagtacata gctccgtacg
     3241 ccggctgcag cattgcggaa tatttcatgt atcagggccg gcatacgctt gtcgtttatg
     3301 acgacctgac caagcaggct gcggcgtacc ggcagatatc gctgctgctt cggcggccgc
     3361 ctggccgcga ggcgtatcct ggcgacgtat tttacctcca cagccgtctt ctcgaacgtg
     3421 cggcaaaact ggcggacaag tatgtagttg cgcccaaaaa tacgcctgat gcggaacttg
     3481 aaaaaattac gcctgtttac accgggccca tcgaaaagaa aaacgcccag gctgctatcg
     3541 acggcatgaa ggacgagtcg ctcgaacttc gcctgctgcc gcagtctggc gggtcgatga
     3601 ccgcactgcc gatttgcgag acccaggaag gtgaagtgtc ggcgtacatt ccgacaaacc
     3661 tgatttccat tacggacggg cagatttatc ttgagccggc gctgtttttc gcaggcgtac
     3721 ggccggcgat aaacgtcggc ataagcgtca gccgcgtcgg ctataaagca gccacgaaag
     3781 ccatgaaaaa agtcgccgca tcgctgcgac ttgacctggc tgcctaccgc gagctggaaa
     3841 gtttcgccca attaggtatg gaacttgacc cggccagcca acgtcagctc gaccgcggcc
     3901 agcgaatggt gcagttgctt acgcagccgc agtcgtcgcc catgtccgta aaagaccagg
     3961 taatctcaat tgcactcggc acgagcggca aacttgacga tatcgatgcg cggtacgtca
     4021 gtacgtttga gaaatatttt ttgacctacc tgcgtgaaag cgaagcggat ttttataagg
     4081 aattctgcga aaatcctgct gagttggatt catcgctcaa acggctggcg tttttggcgg
     4141 aggagtacaa gagtacccgc tgggaacagg atgtcaaaaa cgcccgtaaa gccggcgaag
     4201 tgtagcaagc gaataaaaac tcgattttat tgaaaaaatc ttgaaaagta atggctaaag
     4261 cccgaaaaat attggcccgc acgaaagcga taaaaaatat ccacaccgtc ataaggacga
     4321 tggagatggt ttcgacagcg cggttcaaga aggcatttaa tcgcacttcg tcggcaaggc
     4381 cgtacatcga aggcatggct tcactggtgg aagacatcct catgcgatgt aatccggaaa
     4441 aaatttatca tccgcttttc aggcggccgg aaaaattgcg aaatgcaaac gtcatcttga
     4501 taacgagcga ccgcggcttg tgcggcgggt acaatagtgc cttgtgcaag atggcggagg
     4561 ggcgattgcg ggagtttaca gacggcgacg tgacgacaaa actgtacgtc gtcggcaaaa
     4621 aagggttgac ccatatgcac agagacaggt tcgaggtgat gcacgattat atcgggctcg
     4681 accctgagtc gcagggatgg cagtgggcca gcagccttgg cgacgagttc atgagtcagt
     4741 tcatagccgg ccagcttgac tgcgtcgaag tggtttattc gcgactggtc ggcatgggcg
     4801 gccatcagcc gacgtgtcgt gcactgctgc cgcttgcgtt tcagcttgac gaagacaaag
     4861 acgttaaaaa actcaccatg tctcagcgtg tcgaatatga attcatacct tccgccgggg
     4921 acatgctcga ccatcttctt ccgatgactg tacgccttga attatatcag tggctgatgg
     4981 aagccgccgt taccgaacag atagcgcgca tggctgcgat gcgttcagcc aatgaaaacg
     5041 ccacggaaat gattcgcaaa ctcaccgtac aatacaatcg tacacggcag gctcagataa
     5101 ccacggaact ggctgaaata ctcggcgggg cggtagcgtt ggaaggctga ttttctgtca
     5161 gaattccgca ttgcgcggcg tacgtggata tgggattacg tcgcggatgt tactcatgcc
     5221 ggagcagaac tgcaccgtcc gctcgaggcc aaggccgaaa cctgcgtgcg gcacgctgcc
     5281 gaagcgtcgc aaatccagat accaccagta atcctctttt ttcatgtgca tttcggccat
     5341 gcgggactct atcgcgtcca gattgtcttc ccgctggctg ccgccgatga tttcacctac
     5401 gccaggcacg agtacgtcca ttgccgcgac ggttttgtcg tctttgttgc acttcatata
     5461 aaaaggtttg agagatttgg gatagtcggt gacgattacc ggttttttga ggtattcttc
     5521 ggtcaggtat cgctcatgtt cggtttgcag gtcgcaaccc cagtatgggt tgaaatccca
     5581 ctgtcggttg gcggcttgca ggacttcgac ggcgttggta tatgtaagcc gctcaaaatt
     5641 gctggcggtg atgtgttcca gcgttttcag cagattcgga tttatccgct cgtcaaagaa
     5701 tttcatgtca gcgtcgcaat ccgccaggac tgtgccgaaa atgtgtttca aaaaactttc
     5761 cgcaacggcg gcgtcgcctt cgaggtcgca gaaggccatt tccggttcga ccatccagaa
     5821 ttccgccaga tgtcgtggcg tgtttgaatt ttcagcacgg aacgtcggcc cgaatgtgta
     5881 gacattgccc aggctgcaag catacggttc gacggcaagc tggccggaga ctgtcaaaaa
     5941 tgtcggacgt gcaaaaaaat cctgggaata atccacttgg ccggcttcat tgcgaggcgg
     6001 attgtccatg tcgagcgtcg ttacggcaaa catctcgccg gcgccttcgc agtcgctggt
     6061 tgtaatcagc ggcgtatgaa cgtaaatgaa gccgcgctgc tggaaaaatt cgtgaatcgc
     6121 gctgcatatg cggtttctga cgcgggcgac ggcggaaaat gtgctggttc gcggcctgag
     6181 atgtgcctgc gttcgcagaa attcaaagct gtgtcgtttg ggctgaagcg gatacgccgc
     6241 agcgtcggcg taaccgtata ccgtcaatga acgtgcctgc aattcaacgg tttggccgcg
     6301 gccctgactg gctaccagca cgccggatgc tagcaggctg ctgccggtag tgagcttttc
     6361 cacgtcgccg taattgccga gcgatgaatc ggcgaccacc tgaagcgaat cgaaacatga
     6421 accgtcattc agcgaaatga aactgaaccc gcctttggag tgacggactg tacgaaccca
     6481 tccgccgacc gtaactgtat ctcctggttt atgttgcgac gcgtttaata tctgagatat
     6541 acgttgaacg cttatcattt atcgctcctg atttaatctg gccaatgcat ttggccgata
     6601 ttataatgaa acagttataa atactgaagt ttattgttca aaaggacccc gttttggaaa
     6661 tgcatgttct gcctgaaaaa aaacaaacca tgttctggct gataatcggc acggaaataa
     6721 ccggcatagt cgtaatgatt ggattactgg cgtataagaa attcatcgcc gaccattttt
     6781 ttacatcgca tgagcgtaag ccggctgtca gtgtatctta cacgtcccgg ccgacccatt
     6841 cagcaagacc gctcttcgcc caaaccaggg ttgaatcgga cacaccggtg aaaatcgaat
     6901 cagtgccagc cgccgcagcg gttgcgtgcg ttcaatctca accggctgaa gaagctgtcc
     6961 ctgcgaaact gtcttcagcc agccccgatg ctgcagagcc gtctactcct gaaatttcag
     7021 atttgaacga acagggtgat tcgtcttcct ggcagatttt cagcgaagaa aataaatcgg
     7081 acaaaaactc ggctgccgca gggccgccgt tgccgaaggt cattaaaaac cccaaaataa
     7141 tagtcataaa atccaggcag atactggaag tttatgatgg cggtaaattg ctcaggacat
     7201 atcaggcttc gaccggcagt aatcctggcg acaaggaaaa aagcggcgac cgcaaaacgc
     7261 ctgaaggtga attctatatc tgcgtcaaaa accccaaatc gcgttttgtg ctgtcgctgg
     7321 gcctgagtta tccggaatat gatgatgcaa ggcgcggcct tcgcacgggg cttatcagcc
     7381 agaatgaata cagccacata atttccgaac tcaacatgaa acggcagccg ccgtggaaca
     7441 ccaagctggg aggcgaggtc atgattcacg gcgtacggca aggcggacgt tcgacagccg
     7501 gctgcattgc actggaaaac aatgatattc gcgaacttta tccgcgcata ccaataggca
     7561 ctaaagtcat cgtcaaaccg tgatgtcgat tactggcgat ttttagagca tattaaactt
     7621 gactttgcgc ttgttgctgt ttgcggcgtc ggctg
//
LOCUS       DAONFI010000074         7580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_11242_length_7580_cov_3.755482, whole genome shotgun sequence.
ACCESSION   DAONFI010000074 DAONFI010000000
VERSION     DAONFI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11242_length_7580_cov_3.755482"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PLK08_03600"
     CDS             complement(<1..1332)
                     /locus_tag="PLK08_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPS52618.1"
                     /translation="MSNNALPQDSAARNAIITDIAHTMLVEAAAGTGKTTCMVGRMLS
                     LIRNGIAVENIVAITFTRKAAAELQGRFYRELQANAQNADTSSAERKCFERALTQIDR
                     CYIGTIHSYCASLLREWPFAAGVGMNFAEIDEDADKIIRQQSWNEFIARCFVSDSRKA
                     DWAEKLEEVSLNFNIAKKAFFDFCEYPDVPCWPAENPGLPAAEELKHVTDALKLFQRE
                     LLELQPSLPDNPDAWGNDKLLPQYFRLPGQIDRLNTDNACEVLIFLEDNFSTQLKTGD
                     IVQKNWLDKQQMAKDRLAAWNALCEKIAAPQIKRLFAYRYSLLLELFGQAAEVYGEVR
                     NRRNVLNFQDLLMRTAEMLRSRKDVREYYFRRNMRLLVDEFQDTDPIQAELIFLLTSD
                     NPSCDNWQKCTPKNGSLFVVGDPKQSIYRFRRADIQTYNLVKKKIAKCGVIE"
     gene            complement(1322..4333)
                     /locus_tag="PLK08_03605"
     CDS             complement(1322..4333)
                     /locus_tag="PLK08_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPS52619.1"
                     /translation="MTQRADNSFIASLGEQCRRNMPDEKILVAPTRRIGRQWLQRVCM
                     SGVPIVNVRIETLSSLAMNFAADELSADGRRLLPDNIGPLPVDSLLRENESDWPDSPG
                     LADALYNTIRDLRNSCVDAADLEKAGFETSQRGARLVKILNVYADKIHANGYFDTAEL
                     YLKAAANAPSNWPAESTAICSAAEQTFWTAAQWTFWRSIPQDKKIVSGGSLPLQPAEA
                     RANEQNVSLRNRFDVALKGKSDKVKFFRSLGQAAEIREIFRRCITQKIPLDEVEILYT
                     DRTTYLPLLCEECSAVFGSFDTATFIQGIPAMYSRPGRLVMEWCEWVSSKFSQKSFER
                     MLCDGLFVTGHPSGVLAEKLAALPVGFGRQRWLDVIGGEAELHPDDAVLAELRDIAKR
                     HATLAASLDCDLIKPEILLHAATELVTKHSRCADAFDNLARQRLAAFLSEFTVWYDSH
                     PARRDVSTADRLEWLKSAVKETAVGALGPMSGRIHVSPLHGGGKSGRKHVFIVGMNDA
                     FFPGKGRQDPLLLDDERQNIFEFTHKLLPLSWHMQHESIERLLDFIGEFCGKITLSYC
                     CHDMIDDSEMYPATILLTLAAVLQNAASFSLEELNNLAGQPVAFLPGENTQAVDAARG
                     ILAHYGNDPAKMELLCRRTYPNLQDGYAAAAAWNSVMDFTEYDGNVQPASDDSQPLFS
                     PSGLETLAICPRKFMFVYRLKIQPPDEISPDAETWLNAADFGTLMHDSFCEFYRRRAA
                     AGNFNVTNENTRDELVNIIDTNMKELERRVPVPGENARRTTRRQAFQAMDIFLREEAI
                     YFRECGYRPLYMEAAIGLPPEKIGSTCGTELDRKEPLKISAGNGLFRIRGRIDRIDRD
                     TTRNSYTIWDYKTGSSKKFRNGRFNDGRILQHYLYSLMAGRLISQGEIEHVGYYFTSY
                     RGRGERIKYRPADLDNQGRKILASLSGMVIRGAYPATTDDKDCTYCDYKKICGDTKKL
                     AARMKQMLKECHDEKLDSMRSLRQMEADDEQ"
     gene            complement(4416..5138)
                     /locus_tag="PLK08_03610"
     CDS             complement(4416..5138)
                     /locus_tag="PLK08_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPS52620.1"
                     /translation="MKRYNHLNPVFNVFGGLPPKVEGAGFYYRFMRRVAQVMFGMLWK
                     TRYFNRHFEPAAGSVIYMCNHQSFFDPMLASCALVRPGNYMARESLFKHFGFGNFIRT
                     LNAFPVKRGTADVGALKEAMRRLKDGKTVVIFPEGTRTKNGCIGEFLPGVAMLAQRSA
                     DWVVPVVIDGAYEVWPRNQALPNFTCSIAVEYGQPISHEEAKKMAPQVFVNSVREKMI
                     LMQNELRTRIGRKPFDYSSTLQ"
     gene            complement(5135..5797)
                     /gene="cmk"
                     /locus_tag="PLK08_03615"
     CDS             complement(5135..5797)
                     /gene="cmk"
                     /locus_tag="PLK08_03615"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPS52621.1"
                     /translation="MIVTIDGPAGSGKSTAARKLAQRLGISFLDSGAMYRAVTLKAIR
                     SGTDLRDDFALAREAENIDLKMSPDGDNLLVFLDGVDVSTDIRSEEVSRKTRHAAASP
                     KVRTVLVKLQRKIGRQLGSFVTEGRDQGSVVFPDADFKFYLDAAPAERARRRMLELQA
                     RGENADYNQILAGITARDKSDMSRSEGPLCIPENALIIDSTGKSIDDIQNEMLDIIKN
                     VK"
     gene            5990..6073
                     /locus_tag="PLK08_03620"
     tRNA            5990..6073
                     /locus_tag="PLK08_03620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6024..6026,aa:Leu,seq:cag)
     gene            6273..7310
                     /locus_tag="PLK08_03625"
     CDS             6273..7310
                     /locus_tag="PLK08_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPS52622.1"
                     /translation="MDKQDWYQRYVPDGVIYQRKIDGEYNGPWNYSLYINGRRIQKSL
                     RTTSKALAGRRAAEIYESYANCPAVKMSSLESIIKEFSAHDADIVRPVYNRKREKHIR
                     QFFEVSKLTEVFAITPEQINKYLDSRAAEGIAPKTRKNELSSIGAFCHWAKSRGYLHT
                     NPARDVDAPKVPPMKIVYMQRPELEKALEIAAKNEIWGVFFAYYAGLRVHEIANLKWE
                     QIHVDDNPTIEVRGKGDKIVTLPLHPELVKIIKEKITRVEGRETLFPKHTYRWWDKML
                     EPVRKQCPTLANRGQGWHTFRRTFGSILIQAGVGIERVSRLLRHNQISTTIKHYAHLI
                     PEHGRNDLLNL"
     gene            complement(7379..>7580)
                     /locus_tag="PLK08_03630"
     CDS             complement(7379..>7580)
                     /locus_tag="PLK08_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPS52623.1"
                     /translation="GPKAPESIETQLKRAIRQSGLSLREIARRSGIAHNTINNFIKNQ
                     SVVSIESTDKIFFALGKRIKVL"
BASE COUNT         1712 a         1889 c         2003 g         1976 t
ORIGIN      
        1 ctcgattacg ccgcattttg cgattttctt ttttacaaga ttgtacgtct ggatatccgc
       61 gcggcggaaa cggtatattg attgcttggg gtcgccgacg acgaacagcg agccattttt
      121 gggcgtacac ttttgccagt tgtcgcacga tgggttatca gatgttagca ggaaaataag
      181 ttcagcctgt atcgggtcgg tgtcctgaaa ttcgtcgacc agcagccgca tattccgccg
      241 gaaataatat tcgcgtacat cttttcggct gcggagcatt tctgcggtac gcatgagcag
      301 gtcctggaaa ttaagcacgt tcctgcgatt gcggacttcg ccataaactt cggcagcctg
      361 cccgaaaagt tccagcaaca gcgaatatct gtatgcgaac agccgcttta tctgcggtgc
      421 tgcgattttt tcacacaatg cgttccatgc cgcgagacgg tctttagcca tctgctgttt
      481 atccagccaa tttttctgga caatatcgcc ggttttcagt tgcgttgaaa aattatcttc
      541 aaggaaaatc agcacttcgc aggcgttatc ggtgttcagc cggtcaatct gtccaggcag
      601 ccggaaatat tgcggcagaa gtttgtcgtt accccatgcg tccggattgt ccggcagaga
      661 aggctgaagt tccagcaatt cacgttgaaa caatttgaga gcgtcggtaa cgtgttttaa
      721 ttcttcggcc gctggcaggc cggggttttc agccggccag caaggcacgt caggatattc
      781 gcaaaagtca aaaaacgcct tttttgcaat gttgaaattc agcgaaactt cttcgagttt
      841 ttcagcccag tctgctttcc ggctgtccga cacgaaacag cgcgcgataa attcattcca
      901 ggattgctga cggattatct tgtcggcgtc ttcgtcaatt tcggcaaaat tcattccgac
      961 gcctgcggcg aacggccatt ctcgcagaag gctggcacag tacgagtgta tcgtgcctat
     1021 atagcagcgg tcaatctgcg taagtgcgcg ttcgaagcat ttacgttcgg cagaggaggt
     1081 gtctgcattt tgtgcgttgg cttgcagttc cctgtagaat cggccttgca gttcggcggc
     1141 ggctttgcgg gtaaatgtga ttgcgacgat attttcgacg gcgatgccgt tgcggataag
     1201 gctgagcatc ctgccgacca tgcatgtggt tttgcccgta ccggcggctg cctcaacaag
     1261 catggtgtgg gcgatatcgg ttattatcgc attgcgtgcg gctgaatcct gcggcagtgc
     1321 attattgctc atcgtcagcc tccatctgtc gcagacttcg catggaatcg agtttttcat
     1381 cgtgacattc tttgagcatc tgtttcatgc gtgctgcgag ttttttggtg tcgccgcaga
     1441 tttttttata atcgcaatat gtacagtctt tgtcatcggt ggtagccgga tatgcgccgc
     1501 gtataaccat gcctgagagg ctggcgagta tttttcggcc ttggttatcc aggtcggcgg
     1561 gcctgtattt aatccgctcg cctctgccgc gataactggt gaaataatag ccgacatgtt
     1621 cgatttcacc ttgcgatatc aatcgcccgg ccatgagtga gtagagatag tgctgcagga
     1681 ttctgccgtc gttgaatctg ccgttgcgaa attttttcga gctgccggtc ttgtaatccc
     1741 atatggtgta ggaattgcgg gttgtgtcgc ggtcgattcg gtctatgcgg cctctgattc
     1801 ggaacaatcc gttgccggcg gaaattttca gcggctcttt gcggtcgagt tctgtgccgc
     1861 atgtactgcc aattttctcc ggcggcaggc cgatagccgc ctccatgtag agcggccgat
     1921 aaccgcattc cctgaaatat atcgcctctt cacgtaaaaa aatatccatt gcctgaaatg
     1981 cctgccgccg cgtggtacgt ctggcgtttt cgccaggcac cggcactcga cgttcaagtt
     2041 ctttcatgtt tgtgtcaata atattgacga gttcgtcgcg ggtattttca ttcgtgacgt
     2101 tgaaattgcc ggctgccgcg cggcggcgat aaaattcaca gaacgaatcg tgcatcagcg
     2161 tgccgaaatc cgccgcattc agccatgttt cggcgtctgg cgaaatttca tccggcggct
     2221 ggattttaag ccggtatacg aacatgaatt ttcgcgggca tatcgccagt gtttcaaggc
     2281 cgcttggcga aaacagcggt tgactgtcgt ctgacgccgg ctgaacgttg ccgtcatatt
     2341 ccgtgaaatc catgactgaa ttccatgcag ccgcagcggc gtaaccatcc tgaagatttg
     2401 gatatgtacg gcgacagaga agttccatct tcgcggggtc attgccgtag tgtgcgagta
     2461 tgccgcgcgc ggcgtcgacg gcttgcgtgt tctcgcctgg cagaaatgcg acgggttggc
     2521 cggcgaggtt gttgagttct tccaggctga aagacgcggc gttttgcaga actgcggcga
     2581 gcgtaagcag aatcgtcgca gggtacattt cgctgtcgtc tatcatgtcg tggcagcagt
     2641 agctcagggt tatcttaccg caaaactcgc cgataaaatc caacagccgt tcaatcgact
     2701 cgtgttgcat atgccatgac agcggcagca atttgtgcgt gaattcaaaa atattctgac
     2761 gctcgtcgtc gagcagcagc ggatcctgtc tgcctttgcc ggggaaaaag gcgtcattca
     2821 tgccgactat gaaaacgtgt tttcgtccgc ttttgccgcc gccatgcagc ggactgacat
     2881 gaattcgtcc gctcatcggc cccagagcgc cgacagctgt ttctttgacg gctgatttca
     2941 gccattccag ccggtcggct gtcgacacat ctcgccgtgc cggatgagag tcgtaccaga
     3001 ccgtaaattc cgataaaaat gccgctaacc gttgtctggc aaggttgtca aaagcatcgg
     3061 cacatctgga gtgtttagtt acaagttccg ttgcagcgtg caggagaatt tcaggtttta
     3121 tcaagtcgca atcaaggctg gccgccagtg ttgcgtggcg cttcgcaatg tcgcgaagct
     3181 cggcaagaac cgcgtcgtca ggatgaagct ccgcttcacc gccgattacg tccagccacc
     3241 gttgtcggcc gaatccaacc ggcaatgccg ccagcttttc tgcaagcacg cctgagggat
     3301 ggcctgtcac gaacaggccg tcgcataaca ttcgttcaaa cgatttttgc gaaaatttcg
     3361 acgagaccca ttcgcaccat tccatcacca gccgccctgg acgggaatac attgccggaa
     3421 tgccctgaat gaacgttgct gtgtcaaaac tgccgaagac ggctgaacat tcttcgcaga
     3481 gcaggggcag atatgtcgtt ctgtcggtat acaggatttc aacttcgtcg agaggtattt
     3541 tttgcgtgat gcatcggcgg aagatttctc tgatttctgc agcctggccg agcgagcgga
     3601 aaaatttcac cttatcgctt ttgcctttca gtgcgacgtc aaacctattt cgcagtgaga
     3661 cattctgttc atttgccctg gcttccgccg gctgaagcgg cagactgccg cctgaaacga
     3721 tttttttatc ctgcggaatc gatcgccaga atgtccattg tgccgctgtc cagaaagtct
     3781 gttcggcggc tgaacagatg gctgtgcttt cagccggcca attcgacggt gcatttgcgg
     3841 cggctttgag ataaagttca gcggtatcga aatatccgtt ggcgtggatt ttgtcggcgt
     3901 agacattgag tatttttacc agccgtgctc cgcgttgcga agtttcgaat ccggctttct
     3961 caaggtcggc tgcgtctacg catgaattcc gcaaatcgcg gatggtattg tataatgcgt
     4021 cggctaatcc tggcgagtcc ggccagtcgg attcattttc acgaagcagc gaatcgacgg
     4081 gcagtgggcc gatattgtcg ggcagaagtc gtcggccgtc tgctgaaagt tcatcggctg
     4141 cgaaattcat ggccaacgac gaaagcgtct cgatacggac attgacgatg ggtactccgg
     4201 acatgcaaac acgttgcagc cattgtcggc cgatacggcg ggtgggagcg acgaggattt
     4261 tttcatccgg catattgcgg cgacattgct cgccaagtga agcaatgaac gaattgtcgg
     4321 ctctttgcgt cattagcctc cttcaggcga atcccgaaca tatcggaata cggatatttc
     4381 aaaactggtt gattttcaac ctcagacaga aaaagttatt gcagcgtcga tgaataatca
     4441 aacggctttc tgccgatgcg tgtgcgcagt tcattttgca taagtatcat tttttcgcgg
     4501 acggagttca cgaatacctg cggggccatt tttttagcct cttcgtgcga aataggctgg
     4561 ccgtattcca cggcaatcga acatgtgaaa ttcggcaggg cctgatttct cggccagact
     4621 tcataggcgc cgtcgatgac tacgggcact acccaatctg cacttcgctg ggccagcatt
     4681 gcgactcctg gcagaaattc gccgatacag ccgtttttcg ttcgcgttcc ttcagggaat
     4741 atgacaacgg ttttgccgtc tttcaggcgg cgcatggctt ctttaagtgc accgacgtcg
     4801 gctgtgccgc gtttgactgg aaaagcattc agcgtgcgga tgaaattgcc gaagccgaag
     4861 tgtttgaaca gcgattcgcg ggccatgtag tttcccggcc ggacaagtgc acacgacgcc
     4921 agcatcgggt cgaaaaagct ctgatgattg cacatgtata tcacgctgcc ggctgccggc
     4981 tcgaaatggc gattgaaata ccgcgtcttc cagagcatgc cgaacatcac ctgcgccacg
     5041 cgtcgcatga agcggtaata aaatccggcg ccttcgacct tcggcggcag gccgccgaaa
     5101 acgttaaata caggattgag atgattatag cgtttcattt tacgttcttt attatgtcaa
     5161 gcatttcgtt ttgtatgtcg tcgatacttt tgccggttga atcgattatg agggcatttt
     5221 ccggtatgca cagcgggcct tctgagcggg acatgtcact tttgtcgcga gccgttatgc
     5281 cggcgagaat ctgattgtaa tctgcatttt cgccgcgagc ctgaagctcc aacatacggc
     5341 ggcgagcacg ttccgccggc gctgcgtcga gataaaattt gaaatccgcg tccggaaaaa
     5401 ccacactgcc ctggtcccgg ccttcggtta cgaagctgcc gagctgtctg ccgattttcc
     5461 tctgaagttt caccagcaca gtccgtactt tcggcgaagc tgctgcatga cgggttttac
     5521 gcgatacttc ctcgctacgg atatcggtac tcacatcgac tccgtcgaga aacaccaaca
     5581 ggttatcgcc gtcgggcgac attttcaggt cgatgttttc cgcttcgcgt gcaagcgcaa
     5641 aatcatccct gaggtctgtc ccgctgcgga tagccttcag cgtaacggcg cgatacatcg
     5701 caccgctgtc caggaatgaa attccgaggc gttgtgcaag ttttctggct gccgtgctct
     5761 tgcctgaacc ggccggccca tctatggtaa caatcataac cagcaaagtt tagcaaatcc
     5821 gcgtccgcga tggtagcgga acttttttaa aagatgcgga agataacttg tcggaatttt
     5881 tttgtgtttg ccgctaaagg cggttaaaaa cggtgaagcc gaaaaaaatg ttgactttta
     5941 tgcggcggct tgtaaagtat cctctccgga tatccggagc agactgcccg cggacgtggc
     6001 ggaattggca gacgcgcagg cttcaggtgc ctgttcccgc aagggagtgg aggttcgatc
     6061 cctctcgtcc gcatgcagac aaaaggccga taaactcaat gtttatgggc cttttttaat
     6121 atataggcct cttgcgagcc ctgttttaaa gggttttgtg attttcaaaa tagtccagta
     6181 ttttcctgtt tcttcttgtt ttattgtgag cctttttgca aaatggtgac agtgtttggt
     6241 gacagtgaca agcacgtagg cacgagtaaa tcatggataa acaggattgg tatcagcgtt
     6301 acgttccgga tggggtaatt taccaaagaa aaatcgacgg cgaatataac ggcccgtgga
     6361 attattctct gtacattaac ggccgacgaa tccagaagtc gctccgaacc acgtcaaagg
     6421 ccttggcggg ccgcagggct gccgagatat acgaatcata cgccaactgc ccagcagtca
     6481 agatgtcgtc cctggagtct atcataaaag aattctccgc ccatgacgcc gatatcgttc
     6541 ggccagtcta caaccggaaa cgcgaaaaac atatccgtca gttcttcgaa gtttcaaaac
     6601 ttacggaagt ttttgctatc actcccgaac aaataaacaa gtatctcgat tccagggcgg
     6661 cagagggaat agccccgaaa acccgcaaga acgaactgtc gtcaatcggg gcattttgcc
     6721 attgggcaaa atctcgcggt tatctgcata ccaatcccgc cagagatgtc gacgccccca
     6781 aagtgccgcc catgaagata gtctatatgc agcggccgga gcttgaaaag gcattggaaa
     6841 ttgctgcaaa aaatgagata tggggcgtat ttttcgctta ctatgctggt ttacgtgtgc
     6901 atgagattgc caatcttaaa tgggagcaga tacacgtcga cgataacccg acgatagagg
     6961 tcaggggtaa aggcgataaa attgtaacgc ttccgcttca tccggaactt gtgaaaatca
     7021 tcaaagagaa aattacgcga gttgaaggca gggaaacgtt gtttccgaag cacacttacc
     7081 gatggtggga caagatgctt gaacctgtcc gtaaacaatg cccaacgctg gcgaacaggg
     7141 ggcagggctg gcacacgttc cgtcggacgt tcggcagcat cctgattcag gccggcgtcg
     7201 gtatcgagcg ggtcagtagg ctgttgaggc acaaccagat atctacgacg ataaaacact
     7261 atgcccacct gataccggag catgggcgga acgacctgct gaatctgtaa attttataaa
     7321 atttttttct ggatttttct ataaaaaatt tttgggccat ccgcataata tccaatattt
     7381 acaatacttt tatgcgtttg ccgagggcga aaaaaatttt atcagtggac tctatactga
     7441 ccacgctttg attttttata aaattattta tggtgttatg ggctataccg gaacgccgtg
     7501 cgatttcgcg aaggctcaac ccactctggc gaattgctcg cttgagctga gtttcgatac
     7561 tttccggagc tttcgggcca
//
LOCUS       DAONFI010000075         7412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_11585_length_7412_cov_4.069865, whole genome shotgun sequence.
ACCESSION   DAONFI010000075 DAONFI010000000
VERSION     DAONFI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11585_length_7412_cov_4.069865"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1229)
                     /locus_tag="PLK08_03635"
     CDS             complement(135..1229)
                     /locus_tag="PLK08_03635"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPS52624.1"
                     /translation="MSGVKRVGILTGGGDCPGLNAVIRAVAKPAMNAGLEVYGIEDGY
                     LGLIEDRIRPLSYSDVSGILTVGGTILGSCNKANPADFSVWEDGKWVKYDVRDKVVEN
                     IDRYGLDVIFVIGGDGTMSGAADLIDRGVRMIGVPKTIDNDLCSTEITFGHNTAVTTA
                     SDALDKVHTTASSHHRVMIMELMGRYAGWLTLNSGVASGADVILIPEIPFDINAVAEH
                     CITRSTHGKRFTIIAVGEGATQADGQKFIDHIVENSPDPVRLGGVAKYIADKVEEIAG
                     LDTRSMVLGHVQRGGSPTPRDRILASCFGYHAFELCKAGRFNELVVMNRNEISSVPIK
                     SVANKIRTVPLDHPIIQTAKAVGTSFGVFE"
     gene            complement(1217..2266)
                     /locus_tag="PLK08_03640"
     CDS             complement(1217..2266)
                     /locus_tag="PLK08_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPS52625.1"
                     /translation="MEQLREKRIQTICLMILTAAAVSAGLYFLREVMIPFVLAVFLTL
                     TVSPVVRFFQFRCHVPRYLSLGIVLLCGVVFLGLMWWLVSASFSQLADQFGEYQNSYR
                     DTLDKICRYMGINSETVKSAINSAFNSDDLKIGEIMVTIMRSVMDVVSKGVLVLIFMM
                     FMLVGNSGRVAGGNDEIISQLTGSVQRYLGTKLFTSTTTGILVGLTLWFIGVDMAVVF
                     GLLAFVLNFIPCIGSIIATILPIPLAVMSPGVSAAGVVLTVAVPTCIQFMIGNIIEPK
                     LLGKSLNLHPVTVLLALIFWGFLWGVIGMLLATPITAVLRSSLAGWELTAPVAALMAG
                     DISVLKKTGDEKCQA"
     gene            complement(2364..2801)
                     /locus_tag="PLK08_03645"
     CDS             complement(2364..2801)
                     /locus_tag="PLK08_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52626.1"
                     /translation="MRITAIISMSVVSVIAVLFVGCGSNNEQGIDLRNEGRMAMRIPY
                     GTVSSATFLITETKDGYYKFEKKSYTGDLVIRELGKKPFLIKKGVCLKLERPSSEQRP
                     FVNAMAAVCVARNLNLFVDEPGAQDVTKETDDVTWIVKREQSR"
     gene            2999..4093
                     /locus_tag="PLK08_03650"
     CDS             2999..4093
                     /locus_tag="PLK08_03650"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPS52627.1"
                     /translation="MILKGDKKLPGVIFTALLTAAAMVIFSSQLYSQDSRNSAESAAV
                     TSNPAQPEKIRIQLHWYPQAQFAGFLTAVKKGFFSEEGLDVKIRWGGAGILPLKELKD
                     GNVEFCTAWLSQAIASSTDDNTPCVLLAQQMPKSSMMLITRKKSDINTPVQMTGKKIG
                     IWGGDFSIAPTAFFEKYDIKPVIIMQSASVLPFIRGVVDVASAMYYNEYHKLMEAGIS
                     KDELNIFMLADYGLDFPEDGIYTTADFREKHPDICRKMITAIQKGWQYSFAHKDETID
                     AIIQEATEYNFVTNANHQKWMLDAMEKLIPMKTANSQSWGYLSPSTYANVCEILQRQK
                     LLKNTPPAYVEFYQPAATEPAKTSDGGDAK"
     gene            4090..6150
                     /locus_tag="PLK08_03655"
     CDS             4090..6150
                     /locus_tag="PLK08_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPS52628.1"
                     /translation="MNLTIRTKLILALCIPILLIGIALTVTDYYEQKDLAIKQAKKNY
                     LELVKYHAAALDQNFLIIEQATELNAKIIETFHYTGSNDIDSLLKAGMDSINDIYMAF
                     VAFQPGALQGKNLYAPVLRRENNGELKSSQLQIDEKTGKTRFYPIEDWFLIPQLEQKP
                     SWGEPFVYGAAGQNIICVYSVPFSMNGSFAGIVGSDVMMSNLSEKISTMFQGTGGYGA
                     LVSECGTLISHPDPHLIMSHSIYSLSEENHLPELYEAGLQMRQGKTDILKVRDYKNNS
                     GIKWVAFTPMKSTGWSLVFIIPESKILSPLKSTLMSRTLQTLIILAVVIIMLAIVAYR
                     FTQNLSRMAHIANEIAGGELKIARKSIALLAKDAKNSKRKIADESKRLISSMATMVEK
                     LNSLVSHSQASSRELKESSGTISSQVRELEIASTERAASTNQIVASAKEIAATTQDLV
                     HTMQAVSRATTNTEEIASEGKVSLEKMHDAMTDLTSAAELINDKLTTIHDKAENITCV
                     ITTIVQVADRTNLLSLNAAIEAEQAGENGKGFSIVANEIRRLADQTAAATLHIEKIVK
                     EMLDAVITGVKAMNRFRKDVQEDSDTVNAVSDQMGEIITQVQALGPKFGNVYTGMDSQ
                     SIAARQISQAMEEIGQEAQMNVSALNGLNQIAQTLHHTAEQLQQQVSFFKTTDENDEN
                     DTSS"
     gene            6134..6694
                     /locus_tag="PLK08_03660"
     CDS             6134..6694
                     /locus_tag="PLK08_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPS52629.1"
                     /translation="MIPHPDIAQILVTPERIHQRLDEMAADISATLAGRDVTAIVVMT
                     GAFIFAADLLRRFESIRMHVEPVAIKSYHGQNIRSAGKCEVKLAPSGNIAGRHVLIID
                     DIFDSGLTISILKDMLAQQSPVEIRACSLLYKQRTDLPKRPATPEWYGFEIPDEFVVG
                     YGLDYNGRYRELPYIGVLAEHARNLS"
     gene            6691..7233
                     /locus_tag="PLK08_03665"
     CDS             6691..7233
                     /locus_tag="PLK08_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPS52630.1"
                     /translation="MKFRMSPSPGVVIGKTGEISLAEGLLQDGEIIIFITRASAWSIL
                     LGSFQSIMAFAIAAGVVACCVDGRQLSHETIWSVFITAAAIRLGWAILSWTGRFYVLT
                     NRRIMRISIKNLFRPDVFDCLLVRIENAQIIKSRAEKLLGLGSIVFQISQNPYADVRW
                     QNISNPEKALEIIRKTINKV"
BASE COUNT         2108 a         1943 c         1759 g         1602 t
ORIGIN      
        1 cagccgacgc cgcaaacagc aacaagcgca aagtcaagtt taatatgctc taaagtttaa
       61 aatgctctca ccatcattct caaaaataaa aaataatttt gagaaccgct gctccatacg
      121 gttacgacat cgcgtcactc aaatacgccg aatgaagtgc cgactgcttt ggcggtctga
      181 attatcggat gatccagcgg cacggtacga attttgttgg cgaccgattt tatcggcacg
      241 gatgaaattt cgtttctgtt catcacgaca agttcattaa accgccccgc cttgcacaac
      301 tcaaaggcat gatacccgaa acagctagca agtatgcggt cgcgcggcgt aggtgagccg
      361 ccgcgctgta catggccgag taccatgctg cgcgtatcaa ggccggcgat ttcctcaacc
      421 ttgtcggcaa tatacttggc tacgccgccg agacgaacag gatcaggcga attttcgaca
      481 atgtggtcga taaatttctg gccgtcagcc tgcgtcgcac cttcaccgac tgcaattatc
      541 gtaaaacgtt tgccgtgcgt actgcgagtg atacaatgtt cggcgacggc gtttatgtcg
      601 aacggtattt caggtatcag aatcacgtcc gcacctgaag ccacgccgga attgagcgtc
      661 agccagccgg catagcggcc catgagctcc attatcatca cgcgatgatg gctgctggcg
      721 gtggtgtgaa ccttgtccag cgcatccgat gcggtagtga cggcggtatt gtggccgaag
      781 gttatctccg tgctgcacag gtcgttgtct atggtcttcg gcacgccaat catgcgaacg
      841 ccgcggtcta tcaggtcagc cgcaccgctc atcgttccgt cgccgccgat tacgaatatc
      901 acgtccaagc cgtatctgtc gatgttttca acaaccttgt cccgaacgtc gtactttacc
      961 catttgccgt cttcccatac gctgaaatcg gcaggattag ccttgttgca cgagccgaga
     1021 atcgtaccgc caactgtcag aatgccgctg acatcagaat agctgagcgg ccggatacgg
     1081 tcttctatca agccgagata accgtcttcg atgccgtata cttcaaggcc ggcgttcata
     1141 gccggcttgg caaccgcgcg aataaccgca ttcagtccag gacagtcacc gccgccggta
     1201 agaatgccca ctcgttttac gcctgacatt tttcatctcc tgtttttttc agtaccgaaa
     1261 tatcgccagc catcaaagcc gcaaccggag ccgtaagttc ccatccggca agagacgacc
     1321 gcaaaacagc cgttatcggc gtcgccagca acatgcctat cacaccccat aaaaaccccc
     1381 agaatatcaa cgccagcaat accgtcaccg gatgcagatt cagggatttg cccaaaagtt
     1441 tcggttcgat tatattgcct atcatgaact gtatgcacgt cggcacagct accgtcagaa
     1501 ccacgccggc ggctgaaacg ccaggactca tcaccgccag cggtatcggc agaatcgttg
     1561 cgattatcga accgatgcag ggaatgaaat tcagcacgaa cgccagcaag ccgaaaacca
     1621 ccgccatatc cacacctata aaccacaacg tcaaccctac gagtatgccc gtggttgtag
     1681 acgtaaaaag tttcgtgccc aggtaacgct gtacgctgcc ggttaactgg ctgattattt
     1741 cgtcattgcc gcctgccacg cggcccgaat tgccgaccag catgaacatc atgaatatca
     1801 gaaccagcac gcccttgctg accacgtcca ttaccgaacg cataatcgta accatgattt
     1861 cgccgatttt caaatcgtcg gagttgaatg cagaatttat cgccgatttg accgtctccg
     1921 aatttatgcc catatagcga cagattttat cgagcgtatc gcggtaactg ttctgatatt
     1981 cgccgaattg gtcggcaagc tgcgaaaacg acgccgacac cagccaccac atcagcccca
     2041 gaaatactac accgcacaaa agcacaatgc caagagacag atatcgcggt acatgacatc
     2101 ggaattggaa aaaacgtacc acaggagaaa ccgtcagcgt aagaaacacc gccagtacga
     2161 aaggaatcat cacttcccgc agaaagtaaa gcccggccga gactgccgcc gcggtaagaa
     2221 tcatcagaca tatcgtctgt atgcgctttt ccctcaactg ctccacaaaa gcctcccgta
     2281 agccgtcaac atgaacagac ccacgcagga aaagacaaaa atacactcta ctctatcaga
     2341 aacaactttt accgaacgac gtgttatctg gactgttccc ttttgactat ccaagtcacg
     2401 tcatccgttt ctttagtaac gtcctgagcg ccaggctcgt cgacgaaaag attcagatta
     2461 cgcgcaacac acacggctgc catggcattg acgaacggcc tttgttcgct tgagggccgt
     2521 tccagtttca ggcagacgcc ttttttaatc aaaaagggct tttttcccaa ttcacgaatt
     2581 acaagatcgc cggtatagga ttttttttca aatttgtaat agccgtcttt tgtctctgta
     2641 atcagaaacg tcgccgagct taccgtgcca tacggtatgc gcatggccat tcgcccttca
     2701 ttgcgaaggt cgatgccctg ttcattattg ctgccacagc caacgaatag aaccgcgatg
     2761 acagatacta ccgacatcga tattatcgct gtaattcgca taaccggaac tccttgtcaa
     2821 aaccgccagc ggcggagatg gaatgataac ggcctaatca gccaatatca agaagaatgt
     2881 acgacttatg ggaataagtg acgattttta ttcccaaaca gcggattttt tgttattctt
     2941 ttgggtggct tacagttacg tattttatct atcatccgtg cgttgatgga ggaaaagcat
     3001 gatactcaaa ggcgacaaga agttgccggg cgttattttc acggcattac tgacggcggc
     3061 agcgatggtg attttcagct cccaactata ttcacaggat tccaggaatt ctgcggaatc
     3121 tgcggcagtt acgtcaaatc ccgctcagcc tgaaaaaata cggatacagc tccactggta
     3181 tccgcaggcg cagtttgcag gttttctgac agctgtcaaa aaggggttct tcagtgaaga
     3241 agggctggac gtgaaaatca gatggggcgg agcgggcatt ttgcccctca aggagctgaa
     3301 ggacggaaat gtggaatttt gcacggcttg gctgtcgcag gctatcgcaa gcagtactga
     3361 cgataacact ccatgcgttc tgcttgccca gcaaatgcca aaatcgtcca tgatgctgat
     3421 aacgcgaaaa aaatccgaca taaatacgcc agtacagatg acgggcaaaa aaatcggcat
     3481 atggggcgga gatttcagca tcgcaccgac cgcatttttt gaaaaatacg atataaagcc
     3541 cgtcataatc atgcagtcag ccagcgttct gccgttcata agaggagtgg tggacgtcgc
     3601 ttccgccatg tactacaacg aatatcacaa attgatggaa gccggcatca gcaaagacga
     3661 attaaacata ttcatgctcg cggattatgg ccttgatttt cccgaagacg gaatctatac
     3721 gacagccgac ttcagggaaa aacatcccga catctgccgc aaaatgatta ccgccataca
     3781 aaaaggctgg cagtacagct tcgcacataa ggatgaaacc atagacgcca taatccagga
     3841 ggctacggaa tataatttcg tcacgaacgc caatcaccag aaatggatgc tggacgccat
     3901 ggagaaactc atccccatga aaaccgccaa cagccaatcc tggggatacc tctcgccttc
     3961 aacgtatgca aacgtatgtg aaatactgca acgccagaaa ctgcttaaaa atacgccgcc
     4021 ggcatacgtg gaattctatc agcctgccgc aactgaaccg gcaaaaacct cggacggagg
     4081 tgacgcaaaa tgaatttaac catacgcacg aaactcatac ttgcgttgtg catacccata
     4141 ttgctgattg gcattgcgct gaccgtaacg gattattacg agcagaaaga cctcgccata
     4201 aagcaggcga aaaaaaatta cctggagctg gttaaatatc acgcagccgc cctggatcag
     4261 aattttttga taatcgaaca ggcaacggaa ctgaacgcaa agataataga gacttttcat
     4321 tacacagggt cgaacgacat agactctctc ctgaaagccg gtatggactc cataaatgac
     4381 atttatatgg cttttgtggc atttcagcca ggcgccttgc aaggcaaaaa tctttacgca
     4441 cccgttctgc gcagagaaaa caacggagaa cttaaaagtt cacaattaca gatagacgaa
     4501 aaaaccggca aaacgaggtt ttaccccatt gaagactggt ttctcatacc acagctcgaa
     4561 caaaagccct cttggggcga accattcgta tacggcgcag ccggccagaa cataatatgc
     4621 gtttattcag tcccgttttc catgaacggc agtttcgcag gaatcgtcgg cagcgacgta
     4681 atgatgtcca acctgagcga aaagatatca accatgttcc agggcacagg cggatacgga
     4741 gctctggtaa gcgagtgcgg cactttgata tcccatcccg acccgcatct gataatgagc
     4801 cattccatat acagcttgtc ggaagaaaat catctgccgg agctttacga agccggctta
     4861 cagatgcgac agggcaaaac tgacatattg aaggtacgcg actataaaaa taacagcggg
     4921 ataaaatggg ttgctttcac acccatgaaa tcaacaggct ggtcgctggt gtttatcata
     4981 cctgaatcca aaatactctc gccgttaaaa tccacgctca tgtcacgaac gctgcaaacg
     5041 ctgattatac tcgccgtcgt aatcataatg cttgcaatcg tggcgtatcg cttcacacag
     5101 aatctctcca ggatggcgca tatcgccaac gagatagccg gcggagaact caagatcgcc
     5161 agaaaatcca tcgccctgct tgcaaaagat gcgaaaaact caaaaagaaa aatagccgac
     5221 gaatccaaac gtctcatcag cagcatggcc acaatggtgg aaaaactcaa ctcgctggtt
     5281 tcgcattcac aggcgtcttc gcgggaactc aaggaatcgt ccggcacgat atcttcgcag
     5341 gttcgcgaac ttgagattgc gtccacagaa cgtgccgcgt cgacgaatca gatagtggcg
     5401 tccgccaaag aaatcgccgc cacgacacag gatctggttc acacgatgca ggcggtatcc
     5461 agggcgacga caaacactga agaaatagcc agcgagggca aggtcagcct ggaaaaaatg
     5521 cacgatgcaa tgactgacct cacctccgcc gcagaactta taaacgacaa actcacgacc
     5581 atccacgaca aggcggagaa tataacctgc gtgataacaa cgatagttca ggtggctgac
     5641 aggacgaacc tgctcagcct caatgccgcc atagaagccg agcaagccgg cgaaaacggc
     5701 aaaggttttt ccatagtagc aaatgaaatc agacggctgg cggaccagac ggcagctgct
     5761 acgctgcaca tagaaaaaat cgtcaaggaa atgctcgatg ccgtcataac cggcgtaaag
     5821 gcgatgaacc gttttcgtaa agacgtacag gaagattccg acaccgtcaa tgccgtcagc
     5881 gaccagatgg gcgagataat cacgcaggta caggcacttg gcccaaaatt cggcaatgta
     5941 tacacgggta tggactcgca gtcgattgcc gcaaggcaga tatcgcaagc catggaagaa
     6001 atcggccagg aagcgcagat gaacgtcagc gcgctgaacg gcctgaacca gatagcccag
     6061 acgctgcacc acaccgccga acagctgcag cagcaggttt cattcttcaa aaccaccgac
     6121 gagaatgacg aaaatgatac ctcatcctga tatagcgcag atacttgtaa cgcccgaaag
     6181 gatacatcag cggctggacg agatggctgc cgacatctcc gcaacgcttg ccggcagaga
     6241 cgtcaccgcc atcgtggtaa tgacaggcgc attcatattt gcagccgacc ttcttcgcag
     6301 atttgaaagc atccgcatgc atgtagagcc ggtggcgata aaatcgtatc acggccaaaa
     6361 catcagaagt gccggcaaat gtgaagtgaa gctcgcgccg tctggaaata tcgccggccg
     6421 tcacgttctc ataatcgacg atatcttcga cagcggtctg acgatttcca tactgaagga
     6481 catgctggca cagcagtcgc cagtcgaaat acgcgcctgt tcgctattgt acaaacagcg
     6541 gacggacctg cccaaacggc cagccacacc ggagtggtac ggctttgaaa tacccgatga
     6601 attcgtagtc ggctatggcc tggattacaa cggcagatat cgtgaactgc cctacatagg
     6661 agtattggcg gaacacgcaa ggaatttatc atgaagttca gaatgtcacc gtcgccagga
     6721 gttgtcatcg gcaagaccgg cgagatttcg ctggcggaag gtcttttgca ggatggagag
     6781 attatcatct tcattacccg tgccagcgca tggtcgatac tgttgggttc ttttcagtcg
     6841 ataatggcgt ttgcaatcgc agccggcgtg gttgcctgct gcgtcgacgg aagacaactg
     6901 tcgcatgaga ccatatggag cgttttcata acggcggcgg cgatacggct cggctgggct
     6961 attctgagct ggacgggcag attttacgtt ctgacgaaca ggcgaataat gaggatatcc
     7021 ataaaaaatc tttttcggcc agacgtattc gactgcctgc tcgtgcgaat tgaaaacgcg
     7081 caaataatca aaagccgcgc cgaaaaactc ctgggactcg gcagcatagt cttccagatt
     7141 tcccaaaacc cctatgccga cgtgagatgg cagaacatat cgaatcctga aaaggcgttg
     7201 gaaattattc ggaaaacaat caataaggtt tgaaaaacgt atccggcacg ttttgtccat
     7261 ttgggaacgt ttacgacgag aagtcggcat tttttgaaaa aaagaattag aacctatccc
     7321 aaaaacatgg gcgtcgccga aatcgagcaa ttttgcgggc tggcaaggaa gaaaaatggc
     7381 agcatacttt taaaaaaaac atattctgcc gt
//
LOCUS       DAONFI010000076         7285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_11879_length_7285_cov_4.243292, whole genome shotgun sequence.
ACCESSION   DAONFI010000076 DAONFI010000000
VERSION     DAONFI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11879_length_7285_cov_4.243292"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1285)
                     /locus_tag="PLK08_03670"
     CDS             complement(155..1285)
                     /locus_tag="PLK08_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS52631.1"
                     /translation="MTGLDKFIDCMEYNPGPRPNYELGVWAQTRQEWTEQSPEKVKNF
                     GWNWFESEDAIGLDHREYIDVNYGFFPPFEYEVLEENDRYIISRNSLGVVTKALKEGT
                     VDNVRMCMDEYLEFPIKKPEDFANIKKRLTAGLPQRYPADLDKRIRVWETRDYPLVLG
                     RNCAANGFYWRAREWLGTENLSYAWYDYPEMMHEMMEFFADFIIETSRPVLEKINVEY
                     FCLNEDMSMKSGPLLGPDTFKEFIFPHLKRLIDFFKSHGTKYIVMDSDGNPEQLIPLL
                     LDAGVDVLWPLERASDVNPLEWRKKFGRQLRMWGGVDKRVLAQGPADIKKHLREFIPL
                     IEEGGFIPHVDHLVQPGVTLDNFMYYMDCKRALLEGNYATLD"
     gene            1451..2365
                     /locus_tag="PLK08_03675"
     CDS             1451..2365
                     /locus_tag="PLK08_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52632.1"
                     /translation="MLSQIDVIKKSEQRPGFRTLQKTGLGVEYLENYENHHRWLHSHN
                     YIEMSYILAGQCFHELAGQRFSQAAGTLSIINFNQTHNLETQGAIKVVNIYLDIDKYP
                     LPLLPEKLNCIVPMFLSLHPNFAHNLNRIIQIKFSHPETIESLLRLMHEELSNPREGH
                     NWVLSHYFELFLTECCRQILADDTLLSARSDDDAQFSIPLEKARLFIDKNFSCDITLK
                     QIAATAGYQPNYFCRIFKQYAGLPLFEYIIQRRIEQAMILLRSTDEKVLSVAYRCGFN
                     DLSHFNRCFQKTVGLTPGQYRKSSQPGE"
     gene            complement(2406..3542)
                     /locus_tag="PLK08_03680"
     CDS             complement(2406..3542)
                     /locus_tag="PLK08_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS52633.1"
                     /translation="MKIVHFITRLILGGAQENTILTCLGQLRGGHDVTLITGPAIGPE
                     GELLSWAKTMGFRVEVIDDLRREISPRRDWRSARRLEELLRELQPDVFHTHSSKAGII
                     GRNVAWKLGGMKVVHTIHGLPFNEYNPALSNMLYVYLERRAAKQCHKIICVADAMARQ
                     ALGESIGEPALYTTVYSGMETLPFMTPAPAAVKFRESLDLPDDAVLVTQVSRLAELKG
                     HEYIIEAARDITDPRVHFCFVGDGKLRPEIELAIDDAGMAERFHLTGLLPREAMPAVM
                     QATDILVHCSLREGLARALPQAMLAGRPVISYDVDGAREVVTDDTGILLEPEEVDGLR
                     KAVIKLADNPQLRKKLGDAGCEFCRHRFDWLYMVDEIMKVYEKI"
     gene            complement(3539..5602)
                     /locus_tag="PLK08_03685"
     CDS             complement(3539..5602)
                     /locus_tag="PLK08_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52634.1"
                     /translation="MSAGNFRYVLRYVICPEHAPQDRIAELTEFCVESKIEEVMLLFG
                     AGELAAGYPSIEELKPWVDVCRGVKQSLDSHGIALSIKNPWTTTFHVAGGCRLKPEQN
                     FTLMVNETGGIAKMAACPLCENWRKYLCRTFSYLAAEIQPAAIWIENDWRLDNFEPDT
                     GSSCGCFCDLHLKRFADAVGRSSVTRDEVISAILADGEIHPWRKIWFDLWRQSTTEPV
                     AMLRDAVKKASPQTRLALMSSMPDIHSIEHRDWPSIQAAFGFEPAFLTQPYLPPHTEM
                     PAIVANPAVTRRMLTNLKRPIEVYPGLESSTRNGIYSKSKRYSAMECLESVCYGAAGV
                     TISHYDMMGNGISLDAGFGKMLASIKKRLNVICEFGLDDATAAGAVMLFSPEEVLLRR
                     YDPVASNSCSLDWANTLYILGINFGFRDKVTDTTGRAVFACDQTLRAFSDEDIKKLLG
                     GVLVLDAASIEILQQRGFGEYLGIKTRGWANNDAGYAYEEITDGGRELYGVDCPRMAA
                     RSCSDKILLLDASVGGKMRTEIKTAEGRHVCDGLVTYRNSLGGVVVSTAYPLGRQNYY
                     MGYFNIYRKTLWQELLWELGGGKSRYAMAVTSPMRVYRNRCRQGEFIALLNTTLDDVA
                     EVVIRVGDMDAGAAKRVKMIDSQGQRVDLNVYFPTVLPDGSQRWKIHQPIPAMNIVYL
                     LVENG"
     gene            complement(5745..>7285)
                     /gene="rny"
                     /locus_tag="PLK08_03690"
     CDS             complement(5745..>7285)
                     /gene="rny"
                     /locus_tag="PLK08_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPS52635.1"
                     /translation="CLAGGFIVGGIVVYILKSYTAHLRRKNVENELAAAKTAAQAEAQ
                     KILAQAQAGAKSEFLQRREQFDRETEDTRKELRVEEKRLAKREDTIDQKMNTLSAKER
                     MIANSERAITEKEKALVAKDRHYNDLIAQQKSQLMKIANLTHEEAKTQLFAQLDKELE
                     QEKAAIVEKKLAEARENSEVEAREIILQAIQRYASEQSSDHTVSAVDIPSDDMKGRVI
                     GREGRNIRAFEKATGVDVIVDDTPGVVVCSSFDPVRREVARRSLERLIQDGRIHPTRI
                     EEVVANVQKDVQKEILNAGKAAQLEANVGGLHPKLTELLGRLHFRTSYGQNVLQHSME
                     VAYLSQVIADQLGLNGKLARRAGLLHDIGKAVDHEVEGGHPEIGAELLKRYGERDEVV
                     NAAAAHHGDVEATSVYTPIVLAADAVSASRPGARRESLERYVQRLEKLEGIATSFEGV
                     KGAYAIQAGREVRVIVDPENVDDRLTQKIAHDIAKKVEDEMEYPGEVRVTLIREVRCV
                     DYAK"
BASE COUNT         1691 a         2055 c         1685 g         1854 t
ORIGIN      
        1 caaaaacgac tgcgaatttg tcaattcgct gagtttttgt gacgccgcag atgcacgatt
       61 gccactatca cagcctttaa tctcttttta ttttttgcgt ccgcagggta attgagaacg
      121 gcctctaacc atcccataag agaatcatga aagttcagtc caacgtcgca taatttcctt
      181 caagcagcgc ccgcttgcaa tccatataat acatgaaatt atccaatgtt acgcccggct
      241 gaaccagatg atccacgtga gggataaatc cgccttcttc aatcagcgga ataaattccc
      301 gcaaatgctt tttgatatct gccggcccct gtgcaagaac tcgcttgtca acgccacccc
      361 acatgcgtaa ctgtctgccg aatttttttc gccattcaag cggattaacg tcgctcgcac
      421 gctcaagtgg ccacagcacg tcgacgcccg catccaacaa caacggtatc aactgttccg
      481 gattgccgtc gctatccatc acgatgtatt tcgtgccgtg cgacttgaaa aaatcaatca
      541 gccgtttcag atgtgggaaa ataaattcct taaaagtatc aggcccaaga agcgggccgg
      601 atttcatgga catatcctca ttgagacaga aatattccac gttaattttt tcaagaacgg
      661 gccggctcgt ttcaatgatg aagtccgcaa aaaattccat catctcgtgc atcatttcag
      721 gatagtcgta ccacgcatat gaaagattct ccgtacccag ccattcgcgt gcccgccaat
      781 aaaatccgtt cgccgcacag tttctgccca aaaccaacgg atagtcacgg gtttcccaca
      841 ccctgattct tttatcaagg tcggcgggat atctctgagg aaggccggct gtcagccgtt
      901 ttttgatatt cgcaaaatct tccggctttt taattggaaa ttccagatac tcatccatgc
      961 acatccgcac attatcgaca gtaccttcct ttaatgcctt cgtgaccaca ccaagcgaat
     1021 ttcgcgatat gatgtaccgg tcattttcct ccaatacctc gtactcgaac ggcggaaaga
     1081 atccataatt tacgtcgata tattcacgat gatccaatcc gatggcatct tcactttcaa
     1141 accagttcca gccgaaattt tttacctttt ccggcgactg ctcagtccat tcctgtctgg
     1201 tctgagccca gacacccaat tcgtaattcg gacgaggccc agggttatat tccatgcaat
     1261 cgataaattt atccaaacct gtcataacag agttcctttc cggcagtagc aggcagcctt
     1321 ccaagtcaat ccgacccgaa tacttttcct ttttccgttt ttaaaaggct ctgttttata
     1381 atataatcca ttatatgata aaatactttg cttattttat cctcattttc gatttttaca
     1441 ggattatttt atgttatcgc agatagacgt aattaaaaaa tcggaacaga ggcccggctt
     1501 caggacgctc caaaaaacag gcctcggcgt ggaatatctg gagaattacg aaaatcatca
     1561 tcgctggctg cactcgcaca attacatcga gatgtcatac attctcgccg gacaatgttt
     1621 tcacgaactg gccggccagc gattcagcca ggccgccggt acgctttcca taatcaactt
     1681 caatcaaacc cacaatcttg aaactcaggg ggcaataaaa gtcgtaaata tttatctcga
     1741 tatcgacaaa tatccgttgc cgctgcttcc tgaaaaactt aattgcatcg tgccgatgtt
     1801 tttatcgctt catccaaatt tcgcgcacaa tctgaaccgc ataattcaga taaaattttc
     1861 gcatcctgag acaatcgaat cgctgttgcg gctgatgcat gaagagctga gcaatccgcg
     1921 tgaaggtcat aattgggttt tgtcgcatta ttttgaactg tttttaacag aatgctgtcg
     1981 ccagattctg gcagacgaca ctcttttatc cgccagaagc gacgatgatg cgcaattttc
     2041 aattccgctg gaaaaggcca ggctttttat cgacaaaaat ttcagttgtg acatcactct
     2101 caaacagata gccgcgacgg ccggatatca gccgaactat ttttgcagaa ttttcaaaca
     2161 atacgccggc cttccgcttt ttgagtacat aattcaacgg cgtatcgagc aggccatgat
     2221 attgttgcgg tcaacggatg agaaggttct ttcagttgcc tacagatgcg gattcaatga
     2281 cctttcgcat ttcaacaggt gttttcaaaa aacggtcggt ctcactccag gccaatatcg
     2341 taaatcgtct caaccggggg aatgatttta tttgcaggcg gctgggtttt tgggataggt
     2401 tctagttaaa ttttttcgta gaccttcatg atttcgtcaa ccatgtataa ccagtcgaat
     2461 cggtggcggc agaattcaca gcctgcgtcg ccgagctttt tgcgaagttg cggattgtca
     2521 gccagtttta ttacagcctt ccgcaagccg tccacttctt ccggttcaag cagtatcccc
     2581 gtgtcatccg tcacgacttc ccgcgcgccg tccacgtcat agctgattac cggccggccg
     2641 gccagcattg cctgcggcag agccctcgcc aacccttcac gcaatgaaca atgcaccaga
     2701 atatccgtcg cctgcatcac agccggcata gcctcgcgcg gcagcaggcc ggtcagatga
     2761 aaccgttccg ccatgccggc atcgtcgatg gcaagttcta tctccggccg gagctttccg
     2821 tcgccgacga aacagaaatg tacgcggggg tcggttatat ccctcgccgc ctctattatg
     2881 tattcgtgac ctttcagttc agccagccgc gaaacctggg tgacgagcac agcgtcatcc
     2941 ggcaaatcca gcgattcgcg gaatttcacc gccgccggcg caggcgtcat gaacggcaac
     3001 gtttccatgc cgctatatac cgtcgtataa agagccggct cgcctataga ctccccaagc
     3061 gcctgcctgg ccatggcatc tgcgacgcag attattttat gacactgttt tgctgcgcgg
     3121 cgttcgagat acacgtaaag catgttcgac agtgcggggt tgtactcgtt gaacggcagg
     3181 ccgtgaatcg tatgaaccac tttcatgccg ccgagtttcc aggcgacgtt ccgcccgatt
     3241 atgcccgctt tcgacgagtg cgtatgaaag acgtccggct gcaattcacg cagcagttcc
     3301 tccagccgcc gcgcagaacg ccagtcgcgc cgcggcgata tctcccgccg caaatcgtct
     3361 attacttcca cacgaaagcc catggttttc gcccagctga gcagctcgcc ctcagggccg
     3421 atagccgggc cggtaatcag cgtcacatca tggccgccgc gcagctgccc cagacacgtc
     3481 aggatggtat tctcctgcgc gccgccgagt atcagccgcg ttatgaagtg aactatcttc
     3541 atccgttctc caccagcaga tagacgatat tcatcgcggg gatgggctga tgtatcttcc
     3601 accgttgcga gccgtcggga agaaccgtcg gaaaataaac gttcaaatca acccgttggc
     3661 cctgcgagtc tatcatcttc acacgttttg ccgcaccggc gtccatgtcg ccgacgcgaa
     3721 taaccacttc cgccacatcg tcaagcgtcg tgttgagcag tgcgatgaat tcaccctgcc
     3781 ggcatctgtt gcgataaacg cgcatcgggc tggtcaccgc catcgcatat cgcgactttc
     3841 cgccgcccaa ttcccacagc agttcctgcc acagcgtctt gcggtagatg ttaaaatatc
     3901 ccatgtaata attctgccgg cccagcggat acgccgtcga taccaccacg ccgccgagcg
     3961 aattccgata cgtcaccaag ccgtcacaga catggcgccc ctcagcagtt tttatttcgg
     4021 ttcgcatctt cccgccgaca gacgcatcga gcagcagaat tttatcgcta cagctccgtg
     4081 ccgccatacg cgggcaatcc acgccgtaaa gctccctgcc gccgtctgtt atttcctcgt
     4141 aagcgtaacc cgcgtcattg tttgcccagc cgcgcgtttt tattcccaaa tattcgccga
     4201 atcctcgctg ttgcagaatc tcaatgctgg cggcatcgag cacaagtacc ccgccaagca
     4261 gttttttaat atcttcgtcg ctgaacgcac gcagcgtctg gtcacatgca aataccgctc
     4321 tgcctgttgt gtcggttact ttatccctga aaccaaaatt gattcccaga atgtaaagag
     4381 tgttagccca atccagtgaa caactgttcg acgcaaccgg atcgtaacgg cgaagcagca
     4441 cctcttccgg cgaaaagagc ataactgcgc cggcggcagt cgcgtcgtca aggccaaatt
     4501 cgcaaatgac gttcagccgc ttttttatcg acgcgagcat tttgccgaaa ccggcatcaa
     4561 gcgatatacc attgcccatc atgtcgtagt gacttatcgt aacaccggca gccccgtaac
     4621 agacgctttc caggcattcc atcgccgaat atcgtttcga cttggaatat attccatttc
     4681 gcgtcgaact ttccagccct ggataaacct cgataggccg cttcagattc gtcagcatac
     4741 gccgcgtaac ggcaggattg gcgacgattg ccggcatctc cgtatgcggc gggaggtacg
     4801 gctgagtcag gaacgcaggc tcaaaaccga acgcagcctg aatcgacggc cagtcgcgat
     4861 gttcaatgct atggatatcc ggcatggaac tcatcagtgc aagtcgcgtt tgagggctgg
     4921 cctttttaac tgcgtcacga agcatggcga ccggttcagt cgtactctgc cgccacaggt
     4981 caaaccatat ttttcgccac ggatgaattt caccgtctgc aagaatcgcg gaaatcactt
     5041 catcccgcgt aactgacgaa cggcctaccg cgtcagcgaa tcgtttaagg tgcaagtcac
     5101 aaaaacagcc gcagctacta cccgtatcgg gttcaaaatt atccagacgc cagtcgttct
     5161 caatccagat tgcagccggc tggatttcgg cggctaaata tgagaatgtc cggcacaaat
     5221 attttcgcca attctcgcaa agcggacatg cggccatttt tgcaattccg ccggtttcgt
     5281 taaccatgag cgtaaaattc tgttccggtt ttaaccggca gccgccggcg acgtgaaatg
     5341 tggtcgtcca gggatttttt atgctcaagg caatgccatg cgaatcgaga gattgtttca
     5401 cacccctgca cacatccacc cacggtttca actcttcaat cgaaggatag ccggcagcaa
     5461 gttcaccagc tccaaaaagc agcatgacct cttctatttt cgactcgacg caaaattccg
     5521 tcagttccgc aattctgtcc tgcggtgcat gttcagggca tatcacatag cgcaaaacat
     5581 accgaaaatt tccagccgac atttttttaa aaattctccc aacgtcgttt tgtttttata
     5641 ctgattcccg aaaattctca atttttccaa aacaactgtt atcagatacc tgaaagttaa
     5701 aaattttcag agagccaccg ctccgtctga acaacgccga catcctactt ggcgtagtcc
     5761 acgcatcgaa cttcccttat gagcgtaacg cgtacttcac cagggtattc catttcatct
     5821 tcaacctttt tggcgatgtc gtgggcgatt ttttgcgtca agcggtcgtc gacgttttca
     5881 gggtctacga ttacccgcac ttcacggcca gcttgaatgg cgtatgcgcc cttgacgcct
     5941 tcaaaactgg tggcgatgcc ttcgagtttt tcgaggcgtt gtacgtaacg ctcaaggctc
     6001 tcgcgtcttg cgccgggccg cgaagcgctg actgcgtcgg cggcgagtac gatgggcgta
     6061 tagacgctgg tggcttccac gtcgccgtga tgagcggcgg cagcgttgac gacttcgtcg
     6121 cgttcgccgt agcgtttgag gagttctgct ccaatttcag gatggccgcc ttcgacttcg
     6181 tggtcgacgg ctttgccgat atcgtgcagc aggccggctc tgcgggcaag tttgccgtta
     6241 aggccgagct ggtcggcaat cacctgcgag aggtacgcca cctccatcga atgctgcaaa
     6301 acgttctggc cgtaactggt gcggaagtgc agccggccca gcagttcggt aagtttggga
     6361 tgaaggccgc cgacgttggc ttcaagctgg gcggctttgc cggcattgag aatttccttc
     6421 tgcacgtctt tctgaacgtt agccaccact tcctcgatgc gggtcggatg aatgcggccg
     6481 tcctgaatca accgctcaag actccggcgg gcaacctcgc gacgaaccgg gtcaaacgac
     6541 gaacatacca ccacgccagg ggtatcatcc acgattacat ccacgccggt agctttttca
     6601 aatgcgcgaa tattacggcc ttcacggcca atcacgcgac ccttcatgtc gtcggatgga
     6661 atgtcgacgg cggaaacggt atggtcactg gactgttcag aggcataacg ctggatggct
     6721 tgcagaatta tctcgcgggc ttcgacttca ctgttctcgc gggcttcggc aagttttttc
     6781 tcaacaatgg ccgccttctc ctgttccagc tccttgtcca gctgagcaaa aagctgtgtt
     6841 ttcgcctctt catgcgtaag gttcgcaatc ttcatcaact gcgacttctg ctgcgctatg
     6901 aggtcattgt aatggcgatc cttggcgaca agggcttttt ccttttcagt gatggctcgc
     6961 tcgctgttgg ctatcatacg ttccttggca gaaagcgtgt tcatcttctg gtcaatggtg
     7021 tcttcccgct tggcaagccg tttttcttca accctgagtt ccttcctggt atcttccgtc
     7081 tcgcggtcaa actgttcgcg acgctggagg aactcacttt tggcgccggc ttgagcctga
     7141 gcaagaattt tctgggcttc cgcctgagcg gcggttttgg cggcggcaag ttcgttttcc
     7201 acattcttgc ggcgaagatg tgccgtatat gatttgagaa tgtataccac tatcccgccg
     7261 acgatgaatc cgccagcaag acaac
//
LOCUS       DAONFI010000077         7265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_11931_length_7265_cov_4.378779, whole genome shotgun sequence.
ACCESSION   DAONFI010000077 DAONFI010000000
VERSION     DAONFI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11931_length_7265_cov_4.378779"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLK08_03695"
     CDS             complement(<1..458)
                     /locus_tag="PLK08_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPS52636.1"
                     /translation="MPLTIDSLISRIPSLAECREDILKVRDVLTATFRSGGKLLLCGN
                     GGSAADCEHWAGELLKSFVKDRPLPAKLAEKLPAELAQNLEAALPAIPLGGFQAASTA
                     FANDVAPQLIFAQLTLGLGKPGDVLVGISTSGNSANVCKAVLTAKAIGMKT"
     gene            complement(773..1210)
                     /locus_tag="PLK08_03700"
     CDS             complement(773..1210)
                     /locus_tag="PLK08_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPS52637.1"
                     /translation="MKTLLVCIDFSDKNEKIFTQAKKIAASFKEQIILLHVCQPNPDF
                     LGYEAGTGFYIDQMDDVYKAEAESLKKMAAKLEHDGYNVLPIFVMGQPATEILDNAKK
                     YRVDMILMGTHGHGHLYNLLLGSVAEEILKKAPCPILMVPMKE"
     gene            2452..3783
                     /locus_tag="PLK08_03705"
     CDS             2452..3783
                     /locus_tag="PLK08_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS52638.1"
                     /translation="MTSSRYGNRLFVSAAMLIIAAAGAMAEPTPSSDYRDLKSDYVQT
                     WYIWSDTNCDGILDPGDTKVAAMNNMWTVIGSDESLNIDSTTGNASPTNGYDYNMDTP
                     EKSLVVYMNYSQKDNNWIGSWSNTYEDAYNRQKYGDANGYSIGMVGTGESGTMGVDIM
                     VHNGAGTNRTTYGSSPYKYAKSNPQVMATTGISAALAEPGTGDREVVKYDEVTQSYNE
                     NVNLAMKTWFEVNGGSLDVAAIAAQMDMTEFDAYDTSTWQFSNSSESVFAGLTPDEIA
                     NDPAIQSILTAFAQTVSTYEYQDVFSINADYDTDSNAAGVIGGISGVGSDTDLWADQT
                     VIRLDFNLEDTDFEQIIFYDFGYNSGASQTDPIEIIFKVGLDDEGDKFLYYDVNDDNM
                     YEAGVDTLIDQNRLFISVNSEVPEPATLLLMVCGGAAVVFYRRRGKARMAA"
     assembly_gap    4205..4304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4932..6170
                     /locus_tag="PLK08_03710"
     CDS             4932..6170
                     /locus_tag="PLK08_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPS52639.1"
                     /translation="MAKTNIIKGNAVIGQSGGPSAVINQSLVGVIETLKKSRQIPKIF
                     GANHGVRGIVNNKYFDLRKVSAKELEIIAGTPASALGSSRDKPDEEYCQRIFENFKKN
                     DVRYFFYIGGNDSADAARIVNDMAKASGYDLNCFHVPKTIDNDLRVTDHTPGYGSAAR
                     FVASAFAGEDRDNAALPGIKVSVVMGRDAGWLTASASLAKYCYGNECDENVGPHLVYV
                     PEIAFDKEKFAADVMKAYKKFGRCLIAVSEGIRDAGGTPMAVSLVEAMGREIERDSHG
                     NAQLSGSGALGDALSEYIKKAFGSQKVRVRADTYGYLQRSFAGYASVVDQAEARNCGR
                     KAAELALSGCTDGTVVMVRKKSAKYGVTFERAELKDVARVTKPLDRKFINKAGNGITK
                     AFADYALPLVGGLPRLGRLY"
     gene            6367..6669
                     /gene="gatC"
                     /locus_tag="PLK08_03715"
     CDS             6367..6669
                     /gene="gatC"
                     /locus_tag="PLK08_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPS52640.1"
                     /translation="MPAEITTDIVRHIGQLSRIELTDAEVETFAKQLGDVLKYFDKLN
                     ELDTDGVQPMAHAVELVNVLADDVVSPGLTVEQALANAPAREGNFYKVPKVLGDAS"
     gene            6666..>7265
                     /locus_tag="PLK08_03720"
     CDS             6666..>7265
                     /locus_tag="PLK08_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPS52641.1"
                     /translation="MNCEITNLSAGELAKAIAEGQTTSTAATLAYLDAIEKFDPQLQA
                     FNEVYADRAMKCAAEADAKISAGQNAGPLGGVPIAIKDNLCTTWGHTTCSSRYLENFC
                     APYTATVVERLENAGAVILGKTNLDEFAMGSSTENSGFHVSRNPWDVRKVPGGSSGGS
                     AAAVAGRLCAAALGSDTGGSIRQPASLCGIVGCQPTYGRI"
BASE COUNT         1838 a         1722 c         1895 g         1710 t
ORIGIN      
        1 gttttcatgc cgatggcttt tgccgtcagc acagccttgc atacgttcgc cgaattgccg
       61 ctggtgctga tgccgacaag tacgtcgccc ggttttccaa ggcccagcgt taattgtgcg
      121 aatatcaact gcggagcgac gtcgttggcg aatgcggtgc tggctgcctg gaaaccgccc
      181 agcggaatag ccggcagggc tgcttccaga ttttgagcaa gttcagcggg cagtttttca
      241 gccagtttcg ccggcagtgg ccggtctttc acgaaagact tgagcagttc gccagcccag
      301 tgttcacagt cggctgcact gccgccgttg ccgcaaagca gcaacttgcc gcctgagcgg
      361 aacgtggctg taagtacgtc acgcactttg aggatatctt ccctgcattc cgcaagagaa
      421 ggtatgcggc tgataaggct gtcgattgtt aaaggcattt gcatgtttcc ttgactttgt
      481 gaataaatat taacagtagg taaaccgata tttcacgaat attgtctgcg gctgtccaga
      541 aatttttgcg gaaatttgtt ccttgccgaa ttttattaag gtaactcgat tattctagca
      601 tagttagagt agagtagttc tttggaaaaa gttgttaaaa aaagattatt taatgttgac
      661 agaaattgta tctgtggtag gatgcttata taggctcctc ggtcatgaga acagctctca
      721 aacatgaccg ttcggcgtcg gactcccctc ctccgacgcc ttttttttgc gcttattctt
      781 tcatgggtac cattagaatg gggcagggag cttttttgag gatttcttcc gccacgctgc
      841 cgagcagcag attgtagagg tggccgtggc cgtgagtgcc cataagaatc atgtcaaccc
      901 ggtatttttt tgcgttatcg aggatttcag ttgccggctg ccccatgacg aatatgggca
      961 acacgttata cccgtcatgt tcaagcttag ccgccatctt tttgagcgat tccgcctcag
     1021 ccttgtaaac gtcgtccatc tgatcaatat aaaacccagt accagcctca tagcctagaa
     1081 aatccggatt cggctgacag acgtgcaaca gtattatctg ctccttgaaa gatgcagcta
     1141 ttttctttgc ctgagtgaaa attttttcat ttttgtcgga aaagtcgata cagaccagca
     1201 gtgttttcat ggtatttact ccttttttta cattatacgg gtatgattgg ggttaaacaa
     1261 attctgccat aatataattc tgttttgttt cgaaaaatcg gttatgtgtg tatataaaac
     1321 ggtttgtcat tgtgagccat tatttcacaa atgtaggaac agggctattt ttgtctgtaa
     1381 gtgcttgttt ataatatagt tatgtctttg agtggggtgt gttaagttac cattattcat
     1441 tattaaaatt attaaagtat aaaaaatctt taaaaaaatc gctaaatagg cttgactaaa
     1501 aaggtttttt cgccgatact tatcccatgc agatttgctc atctgcgtcc ggtttgctca
     1561 ccggatagtg ctcattaaaa aatttatttt agttttcgaa aaagcagttg acagccctat
     1621 ggttggggta tatactgctt atatgctcat tgggagaaga agtgctcaga ggtttgttgg
     1681 ctataaaatg ctcaaatgct caaggcatgg aaaaaatcct gccgcactcc tttgactcgg
     1741 actctctcaa taaacacttt ttggatattt gatgtctgta atgcttagca ggcatccagc
     1801 cgaagtgctc aacggctgat tcgaaaaggt aaaatgttgg taaggttgga tctatcaatg
     1861 caaaacaggt gcacctgctc cgtgcgctga ttttacgttt gcattgaatg ttacccttcg
     1921 ccttggaagt ttggatgaga gagatggaaa gaggagaacg aaattttgct cgaacggctg
     1981 tgtttttcac gctcaatgaa aaacacagcc gacaccagac acactcagtg tccggtggtt
     2041 gctcaatctc ttctatttca cagttggcct cagccaaatt ctgtctgtcc cctggcgatg
     2101 aatgcatatg tagcaaaaga ttttgcgcat aggctgcgga cgacggattt tggcatttta
     2161 ggcgttccgc tcagaacgca gaaaggatgg tgaaacggaa ataatgagga gaatgaaccg
     2221 acgctcaatc acgtctcggc tcaaatcctg aaaatgtcgg cgcacaggcg ccgcggaatc
     2281 tgaaaacgga agtacctgaa tcagagacca gtaaggaaag agatagttag attgacatat
     2341 gtgcttgatt gaaaattagg attgctcaat aaaaacggca tcatgctcaa gatgccatga
     2401 aaagtgctca ggactttgcg tataagtccg aacaaggaaa ggaaaaggaa aatgaccagc
     2461 tcaaggtatg gaaacagact ctttgtcagc gcggcgatgc tcattatcgc tgctgcaggc
     2521 gcaatggcgg agcccactcc gagctccgac tatcgtgatc tcaaaagcga ttacgtccag
     2581 acatggtata tctggtccga cactaactgc gacggcatcc tcgatcccgg cgacacgaaa
     2641 gtggcggcca tgaacaacat gtggacggta atcggcagtg acgaatccct caatatcgat
     2701 tcaaccaccg gcaatgccag cccgacgaac ggctatgact acaacatgga cacgccggaa
     2761 aagtcgctcg tggtttacat gaactacagc cagaaggata acaactggat aggttcatgg
     2821 tcgaacacct acgaagatgc ttacaatcgt cagaaatacg gcgatgccaa cggttattcg
     2881 ataggcatgg tcggcactgg cgagtcgggc actatgggtg tggacatcat ggttcacaac
     2941 ggcgccggca cgaacaggac aacctacggc agcagcccgt acaagtatgc caagagcaac
     3001 ccgcaggtga tggccaccac cggcatcagt gcggctcttg ccgagcctgg cacaggcgac
     3061 cgtgaagttg tgaaatatga cgaagtgacc cagagttaca atgaaaacgt caatcttgcg
     3121 atgaagacct ggtttgaagt caacggcggc agcctggacg tggcagccat cgccgcccag
     3181 atggatatga cggaattcga tgcatatgac acctctacat ggcagttcag caactcttcc
     3241 gagagtgtgt ttgccggctt gactcctgat gaaatcgcga acgacccggc catccagagc
     3301 attctgacgg cgtttgccca gactgtcagc acctatgagt atcaggatgt gttctccatc
     3361 aatgccgact atgacaccga cagcaacgca gcaggcgtta tcggcggcat cagcggtgtt
     3421 ggcagcgata ccgacctctg ggctgaccag acagttatac gcctggactt caacctggaa
     3481 gataccgact tcgaacagat tatattctac gacttcggat acaactcagg tgccagccag
     3541 actgacccga tagaaatcat cttcaaggta ggtctggatg acgaaggcga caagttcctc
     3601 tactacgatg tcaacgatga caacatgtat gaggctggag tcgataccct catagaccag
     3661 aaccggctgt tcatctcggt taactccgaa gttccagaac cggcaacgct gttgctgatg
     3721 gtttgcggcg gtgcggcggt tgtcttctac cggagacgcg gcaaagcacg catggctgcc
     3781 tgatgacggc aacgcggtta acccgccgga aatagtccgg cgggaaaatg aaaaagtaaa
     3841 aaaaagctgt ttggcgaact gattagggtt tgcggcgtag agagtctgta aaaaccgaat
     3901 atcggaatta ccctgtgctt gaatgaatcg caagcgccgg tgataaacct ggcagcatgc
     3961 gacgaaatat gagacgcggc gccggcccgc cgataatggc gggtccggca gcccggctta
     4021 aactgaagct gatggcgtct caaacgaaat tcctattcaa aaccttgagc atgcgggacg
     4081 gaatgagccc cgtcccgcac ctttccttcc tcaagggaaa cgaaattcct attcagaaac
     4141 cttgagcacg cgggacggaa tgagccccgt cccgcacctt tccttcctca agggaaacga
     4201 aattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattcag aaaccttgag
     4321 cacgcgggac ggaatgagcc ccgtcccgca cctttccttt ctcaggtgaa cgaaattatt
     4381 gcgttaaaaa atataaaaaa tataaaaaaa atttctgaca gaggagtcat gaatcgggca
     4441 cagggaaggc ttgccgacga gagatgggaa aatactgtat gccggcagcc ttgctgcgag
     4501 gagcgaaagc cggcaatccg cgatggctga aaaagatgtc gaatcgcggg ttgccgtgcg
     4561 gcgtgtgtgt ccattgcagc tttttccgat ggcgataata ggcagccggt ttttcatggt
     4621 tctgagtttt tgagccgtag aaattccgga tgacaaaaaa attttccttt cctttctttc
     4681 caaccaaggc ggagcgggat tgagcccccg ttcgtctgct gttctcctga cgagagatca
     4741 agagatcagc atcgagcctg ccggtgtgag caccaccggc gggctttttt tattttgagc
     4801 cggttttgtg cgaaaatcct tttacttcaa gaaacgtatc aatataatat tcgccgaacg
     4861 aaaaaagtgc cgccgctttt ccctgtatgc aggcggcgga aaaggtattc acagcattgg
     4921 agaccagcat catggccaag accaacatta ttaaaggcaa tgccgttatc ggccagtccg
     4981 gcggcccgtc ggctgttatc aatcagtcgc tcgtgggcgt tatcgaaacg ctcaaaaagt
     5041 cgcggcagat acccaaaatt tttggggcga atcacggcgt tcgtggaatc gtgaacaata
     5101 aatatttcga ccttcgcaag gtcagtgcga aagagcttga aatcatcgcc ggcacgccgg
     5161 cgtcggcact cggctcaagc cgcgacaagc ccgatgaaga atactgccaa cgcatttttg
     5221 agaatttcaa gaaaaatgac gtgcgttact tcttctacat cggcggcaac gattctgccg
     5281 atgcggctcg catagtaaac gatatggcca aggctagcgg ttacgacctc aactgttttc
     5341 acgttccgaa gacgatagac aacgatcttc gcgtgacaga ccacacgcct ggttacggct
     5401 cggcggctcg ttttgtggca agcgcatttg ccggcgaaga ccgcgataac gccgcgttgc
     5461 ctggcataaa ggtatccgta gtcatgggcc gcgacgcagg ctggctgacg gcgtcggcat
     5521 cgctggcgaa atactgctac ggcaatgaat gcgacgaaaa tgtcgggccg catctggtat
     5581 atgtgccgga aatcgcgttc gacaaggaga aattcgccgc tgacgtgatg aaggcgtaca
     5641 agaaattcgg ccgttgtctc atcgccgttt cagaaggcat ccgcgacgcc ggcggcacgc
     5701 cgatggctgt cagcctcgtg gaagcgatgg gcagggaaat tgaacgcgac agccacggca
     5761 atgcgcagct ttcaggcagt ggtgcattgg gcgatgcgtt gtcggagtac atcaagaagg
     5821 cgttcggctc gcagaaagtt cgcgttcgtg ccgacactta cggctatctg cagcgcagtt
     5881 ttgcaggtta cgcttcagtc gtcgaccagg ctgaggctcg caactgcggc cgcaaggctg
     5941 cagagctggc cttgtcgggc tgcaccgacg gcacggtggt aatggttcga aagaaaagcg
     6001 caaaatacgg cgtaacgttc gaacgcgcgg aacttaaaga cgttgcccgc gttaccaagc
     6061 cgcttgacag aaagttcatc aataaggctg gcaacggcat aacgaaagcc tttgccgatt
     6121 acgctctgcc gctcgtcggc ggactgccca ggctgggacg cctctattga gattgctgaa
     6181 aatataaaaa taaatataaa gaacgcccga tgatttttgt gaaaaaatta caaaaacgtc
     6241 gggcgttttt ttattgattc ggcggcatga aaatggcatc cgtgcttgaa agaatgccgg
     6301 caagtcgata gactgacaac tctgtaaatt tgaaatttcc aatttgcaat tttttcggag
     6361 tttttgatgc cggcagagat aacgacagat attgtgcgac atatcggcca attaagccgt
     6421 atcgagctta ccgacgcgga agtggaaact tttgcgaagc agctcggtga cgtattaaaa
     6481 tatttcgaca agctcaatga gcttgatacg gacggcgttc agccgatggc gcatgccgtg
     6541 gaactcgtca acgtgttagc cgacgatgtc gtatcgccgg gcctgacagt cgagcaggct
     6601 cttgccaatg cgcccgcgcg tgagggtaat ttttacaaag ttcccaaagt gttgggagat
     6661 gcctcatgaa ctgcgaaatt acgaatctct ccgccggcga actggcaaaa gccatcgcgg
     6721 aaggccagac caccagtacc gccgcgacgc tggcatacct ggacgcgata gaaaaattcg
     6781 accctcagtt acaggcgttc aatgaagttt acgccgaccg tgccatgaag tgtgccgctg
     6841 aggctgacgc caaaatttca gccggccaga atgccggccc gcttggcggc gtgccgatag
     6901 ctatcaagga caacctctgc accacgtggg ggcatacgac gtgttcgtcc cgctatctcg
     6961 aaaatttttg cgctccctat acggctaccg tggtcgagcg gctggagaat gcaggcgctg
     7021 taatactcgg caaaaccaat ctcgacgaat tcgccatggg ttcgtcgact gagaacagcg
     7081 gttttcatgt gtcgcgcaat ccgtgggacg tgcgaaaagt tccgggcggc agtagcggcg
     7141 ggtctgcggc ggctgtggcg gggcggctgt gcgcggctgc gctcggcagc gatacgggcg
     7201 ggtctatacg tcagccggca agcctttgcg gtatcgtcgg ctgccaaccc acctacggca
     7261 gaata
//
LOCUS       DAONFI010000078         7259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_11947_length_7259_cov_3.760966, whole genome shotgun sequence.
ACCESSION   DAONFI010000078 DAONFI010000000
VERSION     DAONFI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11947_length_7259_cov_3.760966"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLK08_03725"
     CDS             complement(<1..649)
                     /locus_tag="PLK08_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52642.1"
                     /translation="MNRRAFTLLEMLLAVMLMAMVLAVIYGAVWAGVKGRESLAGETD
                     ALCRINAAIDVIQADLQRISNSRTALRIMPLAAGGVIMEISAMPQSDDDTPQKIYVFL
                     MPRSGDVTGKLIRHVEDDSDFADACRAAQTGETILTSAATSTANENFEILLDDVETFR
                     LRCNDGSWIENLYNNRPTLPAIVEITLSTKTSKGKLISVTRCVAIPVQAGPLAIAT"
     gene            complement(646..1107)
                     /locus_tag="PLK08_03730"
     CDS             complement(646..1107)
                     /locus_tag="PLK08_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52643.1"
                     /translation="MSQNRIAMIFFNGRPPFQKNMKKPRNNAFTLIEILAAAIILTAA
                     LAVISASIGNSFSHFRATRLSQAARRIAMEKLELAAADVQTYPAGGTETELATNFDWQ
                     LRLITPGTSTAGVVTRLACDVSWSFRNKTQSLTLQRVILPPDAQTTETTQK"
     gene            complement(1083..1664)
                     /locus_tag="PLK08_03735"
     CDS             complement(1083..1664)
                     /locus_tag="PLK08_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52644.1"
                     /translation="MRHPTDNRKAFTLVELALVIVIMALLAALAYPAIRNSWASFSGR
                     SGTDDFIRLLRQTRLYATQMQLPCEIRFIKTDEGEYKTQVFVFDTGGHSKQLNVAWTQ
                     TATDVPIKLVEIAATATADPQPVKFYPTGVKQTVLLTLNPENDDERVVKISAPSGIIS
                     FGTVDSMAGTTFASEAEKIKKIWQEQCLKTVSQ"
     gene            complement(1648..2064)
                     /gene="gspG"
                     /locus_tag="PLK08_03740"
     CDS             complement(1648..2064)
                     /gene="gspG"
                     /locus_tag="PLK08_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPS52645.1"
                     /translation="MKKAFTLVEIILVVVIIALLSGLVLTKIAGKGKQARIAAAQAQI
                     ANLKSALNNFEIDCGRFPTTAEGLDALITRPSSLGDNVQWRAYLDDNKLPQDPWGKPY
                     IYLCPGTINTDGFDIYSAGPDGQQGTQDDIGNASSN"
     gene            complement(2081..3139)
                     /locus_tag="PLK08_03745"
     CDS             complement(2081..3139)
                     /locus_tag="PLK08_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPS52646.1"
                     /translation="MKRRKKISGIAKKHIAQLMIQLADLLEAGCTLSRSLEAIVRQSP
                     DMPAGRLAESLRRDIANGQSMAAAMENAGGVFSTVDISMIRAAEEGGFLPKTLTGLAS
                     HMASQADTFKQIKARLAYPAMLAVTVVVSIIFLLGYVVPKFTAVYHQTSQTLPGPTQA
                     LLAISDFFEAGWIFILIAVVLIFAAFRAMMRFGRFRLAMDSFVLRLPVYGTTLRCWQM
                     YRFAGTMSLLVNGGVTALQALKLSAGVVGSANVRRDIELIAQSVERGQRISTAMSNSR
                     FFDDTTLEMIFVSETTGRLGNVFARLAAQHHRDFQARVDLMLSLLEPVIIVIMSVIVG
                     LTVTAMLLPVFSMDSLLE"
     gene            complement(3159..4889)
                     /locus_tag="PLK08_03750"
     CDS             complement(3159..4889)
                     /locus_tag="PLK08_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPS52647.1"
                     /translation="MKQNQAKSAADGEFIQKLRLAGIVEEPVLQWAIEESFLTGRGVQ
                     ELLSERLPANEVAIYSALAEFLGVEFVELDGGEMRGDLTEYMGGVYASNNRVAPVEFS
                     DDTLVVATSNAEKLLAASEISIDLGCHVRMVLAAPGQLQRYTRNLYGLGAGTVSQLVG
                     NEGGTDDKSLLLNVTREVAIQEDLDTQQEASLTNFVNQILIEAVKARASDIHIEPSEH
                     QLRVRFRIDGMLHDVPVPPAIKQLEGAIISRIKVLADLDIAEKRLTQDGQIRLRILGR
                     PVDVRVSVLPSIYGESLVLRILDRQSQYRELTEIGMSHEMLESYCEILKQANGLILVT
                     GPTGSGKSTTLYASLNHINSPSRKIVTVEDPVEYRLDGITQIQVKESIGLGFANILRS
                     IVRHDPDVIMVGEIRDDVTANTALSASITGHLVLATVHTNDAITAISRLANMHLPWYM
                     IASALKVVIAQRLVRLICPKCRQAIRDIPPEAIERYPELVGTTLYKPVGCEACRHTGY
                     QGRTGIYEYFVVTEPIAQAIADADRTTIHRLAVEQGMIPLADAGLNLVRQGKTTLEEL
                     YRVTRDVTRA"
     gene            complement(5049..6242)
                     /locus_tag="PLK08_03755"
     CDS             complement(5049..6242)
                     /locus_tag="PLK08_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52648.1"
                     /translation="MSKTGIAIVGASMRSIFMFAYLKENPQQGFITGVYDSIPQRGRY
                     LLDSYGFKDGVIYDSLEQAVNDSRCQAVFIGTPDYAHVEPVRAALAAGKHVYCEKPLA
                     ITLEDCDAIIETAKTAKSVFYLGMNMRHSPVHEKMHQILTSGQLGRLLTIEANEYYVG
                     GRTYFRRWNRLRKFGGGLWITKSCHDFDLLNWFAGGKPRCVFATSSLSYYKSKPDGGM
                     YCRNCPIKTSCPDFFDVNNPDASKWPAQFNELAKITEKETGQMQDMCLYNSDKDTFDN
                     GMAIVEYDNDVRATYTVNVVSARTTRQLRLTGTAAAAEGDMEAARITVWKRYSDEKTE
                     YNLADEVYGSSHGGADSKILDDFFECCRTGKKPRSSWADGRLGIQVALAARNSCDTGQ
                     KISLK"
     gene            6640..>7259
                     /locus_tag="PLK08_03760"
     CDS             6640..>7259
                     /locus_tag="PLK08_03760"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HPS52649.1"
                     /translation="MKAMLNSVVVIALTAGFFFAGCEPEKPVAAKPAASAPSPIDPQA
                     AAVLKNMCQTLDSAKSFSCTVHQIYDSPSASGQLVQYTRKSDVLVERPDSMAVKTVGY
                     DFEKKFWYKGKSMTILDDVSRVYARDENCPDSITGMLDFLADKYHLVMPLADLLFPQA
                     DKCMTADVTSGTYLGVHRCGKYRCHHLAFVQPNIDWQVWIDAGEKPL"
BASE COUNT         1552 a         1942 c         1931 g         1834 t
ORIGIN      
        1 ccgttgcgat tgccagcggg ccagcctgta ccggtatggc cacgcatcgg gtcacgctga
       61 tgagtttacc ctttgatgtt ttcgtcgata gcgttatctc cacaatcgcc ggcagtgtcg
      121 gccggttgtt gtataaattt tcaatccaac tgccgtcgtt gcatcgcaaa cgaaatgtct
      181 cgacgtcgtc aagcagtatt tcgaaattct cgtttgcggt cgacgttgcc gccgaggtca
      241 ggatggtttc gccggtttgg gctgctctgc aggcgtcggc gaaatcagag tcgtcctcga
      301 cgtgtcggat gagtttgcct gtcacatcgc ctgagcgggg cataaggaag acgtagattt
      361 tctgcggcgt gtcgtcatcg gattgcggca ttgcggatat ttccataatc acgccgccgg
      421 ctgccagcgg catgattctc aacgccgtcc tgctgttgga aattctctgc aggtcagcct
      481 gtatgacgtc tatcgcggca ttgatgcgac aaagggcgtc ggtttcgccg gctagcgatt
      541 ctctgccttt tacgccggcc cagaccgcac cgtaaatcac agccagcacc atcgccatga
      601 gcatgacggc taacagcatt tccaaaagcg taaatgcacg acggttcatt tttgcgttgt
      661 ctccgtagtt tgcgcatcgg ggggcagaat gacacgctgg agcgtgaggc tttgcgtttt
      721 gttgcggaac gaccagctta cgtcgcacgc cagccgcgta acaacgccgg cagtggacgt
      781 acctggcgtt atcagccgca actgccagtc aaaattcgtg gccagttccg tttccgttcc
      841 gccggctgga tacgtctgaa cgtcagccgc tgccagttcg agtttctcca tggctatccg
      901 gcgggcagcc tgcgaaagcc tcgttgcgcg gaaatgtgaa aatgaatttc ctatgctggc
      961 ggatatcacg gcgagagcag ccgtcaatat gatggctgct gcaagtattt ctatcagtgt
     1021 aaatgcgttg tttcgcggtt ttttcatatt cttttggaac ggcggcctgc cgttgaaaaa
     1081 aatcattgcg atacggtttt gagacattgt tcctgccaga ttttttttat tttttcggct
     1141 tcgctggcga atgtcgtgcc ggccatgcta tcaactgtgc cgaacgatat tattcctgac
     1201 ggcgccgata tttttacgac tctttcatcg tcgttttccg gattcagcgt gagaagaacg
     1261 gtttgcttca ctcccgtggg atagaatttc accggctgcg ggtcagcagt ggctgtcgct
     1321 gcaatttcga cgagttttat gggcacgtcg gttgcggtct gcgtccaggc tacattaagc
     1381 tgtttggaat gtccgccggt atcgaataca aaaacctgcg ttttatactc gccttcatcc
     1441 gtctttatga agcgtatctc gcagggcagc tgcatctgtg tggcgtacag tcgcgtctgg
     1501 cgcaacagcc gtataaaatc gtccgttccg cttctgcctg aaaacgaagc ccacgaatta
     1561 cgaatggccg gataggcaag agccgccagc aatgccatga ttacaatgac cagtgcaagc
     1621 tccaccagcg taaaagcctt cctgttgtca gttggatgac gcattgccga tatcatcctg
     1681 cgttccctgc tggccgtcag ggccggcgga ataaatatcg aatccgtccg tattgatagt
     1741 gcccgggcac aggtatatgt atggtttgcc ccagggatcc tgcggcaatt tgttgtcgtc
     1801 aagatatgct cgccactgca cgttgtcgcc gagcgaagac ggccgcgtta tgagcgcgtc
     1861 gaggccttcc gccgtggtcg gaaaacggcc gcagtctatt tcaaaattgt tcaatgccga
     1921 tttgaggttt gcaatctgtg cctgggcggc agctatgcgg gcctgcttgc ccttgccggc
     1981 tatcttcgtg agcaccagcc cggaaagcag cgctattatc actacgacca gtattatttc
     2041 gacaagtgtg aatgcttttt tcatgatttc accatccgtg tcattccaga agactgtcca
     2101 tggaaaatac cggcagcagc atggctgtca cggtaaggcc cactattacg ctcattatga
     2161 ctattataac aggttcaagc agcgaaagca tcaggtcaac cctggcctga aaatcgcgat
     2221 gatgctgggc ggctagacgg gcaaatacgt tgcccagcct gccggtggtc tcgctgacga
     2281 atatcatctc cagcgtcgtg tcgtcgaaaa atctcgaatt gctcattgcg gtgctgattc
     2341 gctggccccg ttcgacgctt tgtgcgatga gttcgatgtc gcgtctgaca tttgcactgc
     2401 cgactacgcc ggctgagagt tttaacgcct gcaatgccgt cactccgccg ttcaccagca
     2461 atgacatcgt tcctgcgaat cgatacatct gccagcatct gagcgtcgtg ccgtaaaccg
     2521 gcagccgcag cacgaacgaa tccatcgcaa gacgaaaccg cccgaatcgc atcatggcgc
     2581 ggaatgccgc aaatatcagg acgacggcga tgaggatgaa tatccagccg gcttcgaaga
     2641 aatcgcttat tgcgagcaat gcctgcgttg ggccgggaag cgtctggctg gtctgatgat
     2701 agacggctgt aaatttcggc acgacataac ccagcagaaa aattatggac accacaacgg
     2761 tgacggccaa cattgccgga tatgccagcc gcgcttttat ctgtttgaac gtgtcagcct
     2821 ggctggccat gtgcgacgca aggccggtga gggttttggg gagaaaaccg ccttcttcgg
     2881 cggcacgtat catggagatg tcgacggtcg aaaaaactcc accggcgttt tccatggcgg
     2941 cagccatcga ttggccgttg gctatgtccc gtcgcaacga ttccgccagc cggccagccg
     3001 gcatatcggg cgactgccgg acaatagctt caagcgaacg gctgagcgta cagccagcct
     3061 ccagcaggtc ggccaactgt atcatgagct gggctatgtg ctttttggct attccggaaa
     3121 tttttttgcg tcttttcatg ataataaacg tactgccgtc atgccctggt tacgtcgcga
     3181 gttacgcgat aaagttcttc gagcgtggtt ttgccctgcc tgacgagatt gaggccggcg
     3241 tcagcaagcg gtatcatgcc ctgttcgacg gccagccggt gaatcgttgt ccggtcagcg
     3301 tcggcgatgg cctgcgctat cggctcagtt accacgaaat attcatatat gcccgtacgg
     3361 ccctgatagc cggtgtgtcg acatgcctcg cagccgaccg gcttgtaaag cgtagtgccg
     3421 acaagttccg gatatcgctc aatagcttcg ggcggaatgt cgcgaatagc ctggcggcat
     3481 ttgggacata tcagccgcac aagccgctgg gcgattacca ccttcagggc ggaggctatc
     3541 atgtaccacg gcaggtgcat gttggccagg cggctgatgg ctgtaatagc gtcgttcgta
     3601 tggacggtgg ccagcaccag gtgaccggtt atcgacgcac tgagtgcggt gttcgccgtc
     3661 acgtcgtcgc ggatttcgcc gaccattatc acgtccgggt catgacgcac tatgctgcgc
     3721 agaatgttgg cgaaaccgag gcctatgctt tccttgacct gtatctgcgt tatgccgtcg
     3781 agacggtatt ccaccggatc ttccacggtg acgattttgc gagacgggct gttgatgtga
     3841 ttcagcgaag catataacgt cgtgctcttg ccgctgcccg tcgggccggt cacgagtatc
     3901 agaccgttag cctgcttgag tatttcgcag tacgattcga gcatttcgtg cgacatgccg
     3961 atttccgtga gttcgcgata ctggctttgc cggtcaagga tgcggaggac gaggctttcg
     4021 ccgtaaatgc tcggcagaac cgagacgcgc acgtcgaccg gccggcccag gattcgcagg
     4081 cgtatctggc cgtcctgcgt aagacgcttt tcggcgatat ccaggtccgc cagcactttt
     4141 atacgcgaaa ttatcgcacc ttcgagctgt tttatcgccg gcggcacagg cacgtcgtgc
     4201 aacatgccgt ctatgcggaa tcgcacgcga agctgatgct cggagggctc tatgtggatg
     4261 tccgacgcgc gagccttgac agcctctatc aatatctgat tgacgaagtt cgtcagcgag
     4321 gcttcctgct gcgtgtcgag gtcttcctgg atagccacct cccgcgtgac gttgagaagc
     4381 agcgacttgt catccgtgcc accttcgttg ccgaccagtt ggctgacggt tcctgcgccc
     4441 aagccgtaga gattccttgt gtatcgctgt aattgtccag gcgccgccag caccatgcgc
     4501 acatggcagc cgaggtctat ggatatttca ctggcggcta acagcttttc cgcattggac
     4561 gtggctacga cgagcgtgtc gtcagaaaat tccaccggcg ccacgcgatt attcgacgca
     4621 taaacgccgc ccatgtattc cgtgaggtcg ccccgcattt caccgccgtc cagttcgacg
     4681 aattcaaccc ccagaaattc agccagtgcg gaatagattg ccacttcgtt cgccggcagt
     4741 ctttcgctga gcagttcctg tacgccgcga ccggtcagga aactttcctc gatagcccat
     4801 tgaagaacgg gctcttcgac gattcctgcc agacggagtt tctggataaa ttctccatcg
     4861 gcggcggatt tggcctgatt ctgtttcatg tcgcatatgt taacagaaat cagctggaaa
     4921 aaccctaaaa aatgaatatt ccggacaata ttgtaacgat gcggcggcta atttgcggcg
     4981 tgcggcgcag gacgttatga atatcgggaa tttttgtcag tcagggcagg ctgtcatgga
     5041 acagcggttc atttcagtga tattttctgg cccgtatcgc acgaatttct cgcagccagt
     5101 gccacctgga tgccaagccg tccgtcagcc caactgctgc gaggtttttt gccggtgcgg
     5161 cagcattcaa aaaaatcgtc caatattttt gaatccgctc cgccgtgcga agaaccgtat
     5221 acctcgtcag ccagattata ttccgtcttt tcgtccgagt agcgtttcca gactgttatt
     5281 ctggcagctt ccatatcgcc ttccgcggca gcggctgtgc cggtcagacg aagttgacgt
     5341 gtggttctgg cgctgaccac gttgaccgtg tacgtcgccc gcacgtcatt gtcgtattcc
     5401 acaatcgcca ttccgttgtc gaaagtgtcc ttgtccgaat tgtataaaca catatcctgc
     5461 atctggccgg tttctttttc ggttatcttc gccagttcgt taaactgggc cggccacttg
     5521 gatgcgtccg gattgtttac gtcgaaaaaa tcggggcatg aggtttttat cgggcaattc
     5581 cggcagtaca tgccgccgtc gggtttggat ttgtagtaac tgaggctgga agttgcgaaa
     5641 acgcagcgcg gcttgccgcc ggcgaaccag ttcagcaaat cgaaatcgtg acacgacttg
     5701 gttatccaca atccgccgcc gaattttctg agcctgttcc accgccgaaa atacgttctg
     5761 ccgccgacgt aatattcatt cgcctcgatg gtcaacagcc tgcccagctg gccgcttgtg
     5821 agaatttgat gcatcttttc gtgaaccgga ctgtgccgca tgttcatgcc aagataaaaa
     5881 acgcttttag ccgttttagc cgtttcaatt atggcgtcgc aatcttcaag cgtaatcgca
     5941 agcggttttt cgcaatacac atgtttgccg gctgccaggg ctgcacgtac cggttcgaca
     6001 tgtgcataat cgggtgtgcc gatgaacacc gcctgacaac ggctgtcgtt caccgcctgt
     6061 tcaaggctgt cgtaaatgac gccgtcctta aaaccatatg aatccagcag gtatctgccc
     6121 cgctgcggta tggaatcata tacgcctgtt atgaaaccct gttgcggatt ttccttgaga
     6181 tatgcgaaca tgaaaatgct gcgcatgctc gccccgacaa tagcgatacc tgtctttgac
     6241 atttttttcc ctggaatcgt aaaataaatt ttttatgcgg catacggctt tcgtcgttcc
     6301 gccgttttca gcctacgtac gaaacacaat aaataatttc taaatttcat tataagtctt
     6361 tatcgcttca tttcatatgt gaaaaacgcg aaatcagctc aatttgaacc gtctgctaac
     6421 cattccagcc gtcgactgtt cctgcctgcg aaccgccaga cacattttgc tttattttgt
     6481 aattttcgga atggtataat tatacctctt acgggtttgt ctgccgaaat aaacccgtcg
     6541 ccataccgtt taatatatgc ggccaaccgc ataaggcgat tatttaaaag atgaataata
     6601 ggacataatt gttgaatata aatttgaaag ggatttgcca tgaaagccat gctgaactct
     6661 gttgtcgtca tagcgttgac ggcgggattt ttctttgccg gctgtgagcc ggagaaacct
     6721 gtcgccgcca agccagccgc cagtgcccct tcgccgatag acccacaagc ggcagccgtc
     6781 cttaaaaaca tgtgccagac tcttgactcc gcaaaatcat tcagctgcac tgtgcatcag
     6841 atatacgatt cgcccagcgc atcaggacaa ctcgtgcagt acactcgaaa gagcgacgtg
     6901 cttgtagagc gtccggacag catggcggta aagacggtcg gctatgattt cgagaaaaaa
     6961 ttctggtaca aaggcaaatc catgaccatt ctggacgacg tatccagggt gtacgccagg
     7021 gatgaaaact gtccggacag cataaccggc atgctggatt ttctggcgga taaatatcat
     7081 cttgtgatgc cgctggcgga tttgctgttc ccgcaggctg ataaatgcat gactgccgat
     7141 gtgacttccg gaacatatct gggcgttcac cggtgcggca agtacagatg tcatcatctg
     7201 gcgttcgttc agccgaatat cgactggcag gtttggattg acgccggcga aaagccgct
//
LOCUS       DAONFI010000079         7200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_12082_length_7200_cov_4.391323, whole genome shotgun sequence.
ACCESSION   DAONFI010000079 DAONFI010000000
VERSION     DAONFI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12082_length_7200_cov_4.391323"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLK08_03765"
     CDS             <1..574
                     /locus_tag="PLK08_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52650.1"
                     /translation="GYFHLRFKNRRITKDILKYCLVIWGIVCLLGLIAIVGLVSYRFG
                     WGNHASTETASAHDVRFILNWCKLGSGRIEKVVHSYKSARSFTGDHLDAHAIKITTVD
                     PKELVPNNYGSGWYRCDEAKGALKDAINFICNWKEDDISWFPTANELHSSEIYVYPQS
                     IYYNGTQPTAADLIFVRPKDKMLFFISSKM"
     gene            complement(812..1081)
                     /gene="rpsT"
                     /locus_tag="PLK08_03770"
     CDS             complement(812..1081)
                     /gene="rpsT"
                     /locus_tag="PLK08_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPS52651.1"
                     /translation="MANTASAKKRMRQDVKRRALNRWRKDQVKGSLKKFEESLKTADE
                     AKMKEALSACFKALDKVAAKGTIHKNAANRKKARLSVKLNKAVKK"
     gene            1362..2315
                     /locus_tag="PLK08_03775"
     CDS             1362..2315
                     /locus_tag="PLK08_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52652.1"
                     /translation="MLKKICIILLMAAACGCEEQKPAQMEYPTASLSTPIHETSWGKE
                     GICLQTPHYKIYTTIEGRRIDVVLPGFMESAYHLYEYLTNSSPNPDYTLPMYMLASRQ
                     EWAIMTQKLFGNIGPDNFIENGGYTYRGVTVCYDIGGMNTFSVAAHEGLHQYLWYFYK
                     NRLPLWAEEGLATQCEGFITTNTSVRFTPQENMMRFDSLRTAIKNNDWLPIEKLLVTT
                     SRKQLGQGELYGLGYYGQLYVLVRFIRNHKIYGPRWAKMLDDAKNGKFSKIFGDKTMT
                     LSPAAYYQAIAVKSFRHYINPDLQAFEREFHDYAQKVVIKP"
     gene            complement(2402..2947)
                     /locus_tag="PLK08_03780"
     CDS             complement(2402..2947)
                     /locus_tag="PLK08_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPS52653.1"
                     /translation="MKELFCEDPTYLYIAVTILELLLLFIYFKTRSVRFAMSLLIPVA
                     ICWITFALDYFIVTDREQITLITREIASAVVKDDFKPAEKYLDDNYNGYGGDRSQIIR
                     LAEGVIKKIQIKSCRLTDFNIRIVGRHANMTVVSTLRTDGAMGDGVYCLRWTVYWVKT
                     NADWRIRSVEEPRQTLPGFGK"
     gene            3131..3538
                     /locus_tag="PLK08_03785"
     CDS             3131..3538
                     /locus_tag="PLK08_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPS52654.1"
                     /translation="MSSYQETLELKTAAHDAMVDITTQVEVAVYNSGIKNGQVMVFCP
                     HTTAAITINENTDPNVTDDIIQKLTRLCPWREEFYSHIEGNSAAHVKSSLVGVSEVIP
                     LADGRMQLGTWQGIYFCEFDGPRKRKFIVTVTS"
     gene            complement(3557..4345)
                     /locus_tag="PLK08_03790"
     CDS             complement(3557..4345)
                     /locus_tag="PLK08_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS52655.1"
                     /translation="MTMKIISGLISASLMLAAGCELGIWPDSAQKSLTAANDAYRADN
                     YSLTVDEASKAMKSGSASQKAYEAQYLRGMAMLKLGKTGQAEDDLQNVSKRAKNLDLQ
                     VRAMDSLAEVYYQQSRFAPAERMFKDVIDNTMPEKQPCDHAQYRLGCIAQRLGRWQEA
                     DVHLQKVIFLFPNSNYASLARAVAGSTFWSIQAGVFDSRQQVDLAAKKYANAKLISYV
                     QPVLVDGKLRFVLLFGKWKTWNDADSAMTAHEQFFKDATIVPAK"
     gene            complement(4350..5231)
                     /locus_tag="PLK08_03795"
     CDS             complement(4350..5231)
                     /locus_tag="PLK08_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPS52656.1"
                     /translation="MNKKFQKILRSAVAESLGRYEYSPSEDTLRVQMCDIGRRMYERG
                     YVAANDGNLCCRLGNGRFLCTPTGVSKGFMKPHMLCIVDENGCQLEGEWPCTSEIKLH
                     LAIMREVPHIHAVAHAHSPYASAFAVAGRPVPPALMPEVEALLGPVPTAPFALPGSKE
                     LAESVIPFVRNNANTVLLSNHGAVGCDVTLEAAYFHLETLDSYLRTVVLAEKIGTPKP
                     ISEQAVAGLLEWKRKNNVPDPRLPAGAVKTDIIPPAMKPAGESSVDALTQEILRRLSE
                     KIEESPELLKIWQSVKD"
     gene            complement(5234..6445)
                     /gene="tyrS"
                     /locus_tag="PLK08_03800"
     CDS             complement(5234..6445)
                     /gene="tyrS"
                     /locus_tag="PLK08_03800"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPS52657.1"
                     /translation="MTQNDTTNNSVAASLDILLRGAEAVYSKDELASRLLSGKPMRVK
                     LGLDPTSPDIHLGHTVVLRKLRQFQDLGHKAVMIIGDYTARIGDPTGVNRTRPMLTPE
                     QIKKNAETYFQQAGRILNTSPDKLEVRYNSEWLAKLSFADVIRLASQMTVAQMMERDT
                     FEKRYKAGQPIGVHEFFYPLMQGYDSVMIEADVELGGTDQTFNNLVGRDLQINAGQQP
                     QIVMVMPILVGLDGVEKMSKSKGNYIGVTDEPNDMFGKIMSIPDSLMENYFTLLTNLP
                     MDEIKTLCDPDKTHPRQAKARLGRMIVENFYSLPAAQAASDEFDRVFAQKNVPTDMPE
                     ISIKTPINIIELVTQAGFAQSNGEARRLIQQGAVSINDEKISELDSKVLPSTGDVLKV
                     GKRRFARLVVN"
     gene            complement(6478..>7200)
                     /locus_tag="PLK08_03805"
     CDS             complement(6478..>7200)
                     /locus_tag="PLK08_03805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPS52658.1"
                     /translation="PAKLLDFGDAHVFGIGSKKICEKKMKYEAVVFDLDGTLLNTLED
                     LADCMNATIGEFGYAPQPLENYRYYVGDGVVNLARRACCDSDISEETIKKIVARNREI
                     YGNGWAVKTRPYSGIPELLDELAARGVKMSVMSNKPHQFTRLCVERFFDIDKFVVVQG
                     VMDGIAPKPDPAGVDMIVKTIGLPREKFLYLGDTNTDMKTAVAAKMFAVGATWGFRTA
                     EELLKYGAMKLVDRPEELLKLL"
BASE COUNT         1816 a         1857 c         1677 g         1850 t
ORIGIN      
        1 cggatacttt catctacggt ttaaaaaccg tagaattacg aaggacatat taaaatactg
       61 cctcgtaata tggggaatcg tctgcctgtt gggtttgata gccatagtcg gacttgtttc
      121 ataccgattt ggctggggaa atcacgcctc aaccgaaaca gccagcgctc atgacgtaag
      181 atttattctt aactggtgca aactcggcag cggcagaatc gaaaaagtcg tccacagtta
      241 caaatccgca cgttcattca caggcgacca tctcgatgct catgcaatta aaataacgac
      301 ggtagacccc aaggaactgg tgccgaataa ctacggttcc ggctggtaca ggtgcgacga
      361 agcgaaaggc gcactgaaag acgctattaa tttcatctgc aactggaagg aagatgacat
      421 ttcatggttt ccgacagcaa atgaacttca ttcctccgaa atatacgttt atccgcagag
      481 tatatactat aatggaacgc agcctactgc tgcagacctt attttcgtac gtcctaaaga
      541 taaaatgctg tttttcataa gctcaaaaat gtaaaacggg aattgccata aaaaaaccag
      601 ccaatgcttt taaaagcagg ctggttttaa aaatgtcttt cataattcta cgatttttgg
      661 agtctcgccg aaaaaacttt ttttgagaac tgccataaac cgtagataaa agcatccgcc
      721 gtcaggcgga caggcgtaag cctgataaat atgatgcggc tgatttcaat gggtaaaaaa
      781 tcattttccc acaaaaattt cttgccgccg attatttttt gacggctttg ttcaatttga
      841 cggacaggcg ggccttctta cgattagctg cattcttgtg aatagtgccc ttggcggcta
      901 ctttatccag cgccttgaag caggcggaaa gagcctcttt catcttggct tcatcggctg
      961 ttttgagaga ttcttcaaac tttttcagcg aaccctttac ctgatccttg cgccaacggt
     1021 tcaaggcgcg acgttttaca tcctgacgca tgcgtttttt ggctgatgca gtatttgcca
     1081 cagttattct cctggctatt tttaacctgt aaatcttccg tatcaaatat attacaacgg
     1141 catttcgcgt gggactgttc tttacgcgaa atcatccgac aggcgagaaa ttataaacgt
     1201 atttccagcc agagcaagtg ttttttcatt attttagcca ttatctgcat gaataacgct
     1261 ttttgacacc tcctcgtcaa aaactgtagt tttaacgcca gccaatgctg gaattccctg
     1321 taagtttagt gtaatattcg tggattcata aggataaggc catgttgaag aaaatttgca
     1381 ttattctgtt gatggcggca gcatgcggct gcgaagagca aaagccggca caaatggaat
     1441 atccgacggc gtcgctatcg accccaatcc acgaaacgtc gtggggcaaa gaaggcatct
     1501 gcctccagac accacattat aaaatatata ccacaataga aggccgacgg atagacgttg
     1561 tcctgccggg tttcatggaa tcggcctacc acctgtatga atatctgacg aattcttcgc
     1621 caaatccgga ttacaccctg ccgatgtata tgctcgcctc acgccaggaa tgggcgataa
     1681 tgacgcaaaa actcttcggc aacatcggcc ccgataattt catcgagaac ggtggatata
     1741 cgtatcgcgg cgtaacggtc tgctatgata tcggcggcat gaatacgttc agcgtcgcag
     1801 cccacgaagg ccttcatcaa tatttgtggt atttttataa aaatcggctg ccgttatggg
     1861 cagaagaagg cctggcgacc cagtgcgaag gttttatcac cacaaatacg tccgtccgct
     1921 tcacgccgca ggagaacatg atgcgattcg actccctgcg gacagccatc aaaaataacg
     1981 actggctgcc gatagaaaaa ttgctggtaa caacgtccag aaaacagttg ggacaaggtg
     2041 aattgtacgg cttgggttac tacggccagc tttacgtgct ggtccgtttc atccgcaatc
     2101 acaaaattta cggcccacga tgggcaaaaa tgctcgacga cgccaaaaac ggaaaattct
     2161 caaaaatctt cggcgataaa accatgacgt tatcgccggc ggcatattat caggctatcg
     2221 cggtaaaatc gttcaggcat tacatcaatc cggacttgca ggcttttgaa cgggaattcc
     2281 acgattacgc gcagaaagtc gtcataaaac cgtaagccgt tgactcgtgt tcaaaagtac
     2341 atacctgtgc ggctaacaat cattaaataa gccagtttaa catttgaaat ctgtccgatg
     2401 attactttcc gaaacctggt aaagtttggc gaggctcttc aacacttcgt attcgccagt
     2461 cggcattcgt tttaacccag tagacagtcc atctgagaca atataccccg tcgcccatcg
     2521 ccccatctgt ccgcaaagtg cttacaaccg tcatatttgc atgacgaccg acaatcctga
     2581 tattaaaatc ggtcaatctg caacttttaa tctgaatttt ctttatcacg ccttcagcca
     2641 gcctgattat ctgtgacctg tcaccgccat atccattata gttatcgtcc agatattttt
     2701 cagccggctt aaaatcatcc tttacaacag ccgacgctat ttcacgcgta atcagtgtta
     2761 tctgctcgcg gtcggtaaca atgaaataat ccaatgcaaa cgttatccag catatcgcta
     2821 ccggaatcag taacgacatc gcaaacctga cactgcgggt tttaaaatat ataaacagca
     2881 aaagcagctc aagaatcgta accgcaatgt aaagatacgt cggatcttca caaaaaagtt
     2941 ctttcatcac tgactccttt ttttgataac cacaagacga tttactttgt ccggcaggtc
     3001 tggtatactt tgaattcatc ctcaaaacct tggctgcctg caatcggcaa ttttgcagcg
     3061 acacatatgt ttggggatag gttcttataa aaatattcta aatatacagg catagatata
     3121 cgagggaaaa atgtcttcat atcaggaaac gctggaattg aaaacggcgg ctcacgacgc
     3181 catggtcgat atcaccactc aggtggaagt ggctgtttac aacagcggca taaagaacgg
     3241 gcaggtaatg gttttctgcc ctcacacaac ggcagccatc accatcaatg aaaataccga
     3301 cccgaacgta acagatgaca tcatccagaa attgacacgt ctgtgtccat ggcgggagga
     3361 attttactca catatcgagg gcaacagcgc ggcgcacgtc aaaagttcgc tcgtaggcgt
     3421 aagcgaagtt atcccgctgg ctgacgggcg gatgcagtta ggcacatggc agggaatcta
     3481 tttttgtgaa ttcgacggcc cgcgaaagcg gaaattcatc gtaaccgtca caagctgaac
     3541 agtcctgaaa agtcattcat ttcgcaggca cgattgttgc atctttaaaa aactgttcgt
     3601 gggcagtcat cgctgaatct gcgtcgttcc atgttttcca tttgccaaaa agcaggacga
     3661 accgcagttt gccgtcaaca agaacaggct gaacatagga tatgagtttg gcattggcat
     3721 attttttcgc ggcgaggtcg acctgctgcc gcgaatcaaa aacgccagcc tgtattgacc
     3781 agaaagtact gcctgcgacg gctctggcta gcgaggcata atttgaattc gggaacagga
     3841 atatgacttt ctgcaaatgg acgtcggctt cctgccatcg gccgagtcgc tgcgctatgc
     3901 agccgagccg atattgcgcg tggtcgcacg gctgtttttc gggcatggta ttatcgataa
     3961 cgtccttaaa cattctttca gccggcgcaa accggctctg ctgataataa acctcagcca
     4021 gcgaatccat cgcccgcacc tgcaggtcaa gatttttggc gcgttttgaa acattctgaa
     4081 ggtcgtcttc agcctggccg gtcttgccga gcttcaacat cgccatgccg cgcaaatatt
     4141 gcgcctcgta agccttctgg ctggctgacc cggatttcat agccttcgac gcctcgtcga
     4201 cggtcaacga ataattgtca gccctgtagg catcgttagc cgccgtcaac gatttctgcg
     4261 cactgtccgg ccatatgccc aactcacaac cagccgccag catcagcgac gccgatatca
     4321 aacctgagat gattttcata gtcatcgcgt cagtccttta cggattgcca gattttcagc
     4381 aattcgggag attcttcaat tttttcactc agccgccgca gtatttcctg cgtcaacgca
     4441 tcgacactcg actcgccagc cggcttcatg gcaggcggaa taatatctgt cttcacagcc
     4501 cctgccggta gccgtgggtc aggcacgttg tttttacgct tccattcaag aaggccggcg
     4561 acagcctgtt cgcttatcgg cttcggcgtg ccgattttct cagccagcac gactgtccgc
     4621 aaatatgaat cgagcgtttc aagatgaaag tacgccgcct caagcgtgac atcgcagccg
     4681 acagcgccgt gattgctcag caggacggta ttggcgttat ttctgacaaa aggtatgacg
     4741 gactcggcaa gttctttcga acccggcagc gcaaacggcg ccgtaggcac aggccccagc
     4801 agagcctcga cctccggcat taacgcaggc ggcacgggcc ggccggccac tgcgaacgcc
     4861 gacgcatacg ggctgtgcgc atgcgcgacg gcgtgaatgt gcggcacttc acgcataatc
     4921 gccagatgca attttatctc cgacgtgcac ggccattcgc cttcaagctg gcagccgttt
     4981 tcatcgacta tacagagcat gtgcggcttc atgaaacctt tggaaacgcc agtcggcgtg
     5041 cagagaaaac ggccattccc gagccggcag cagagattcc cgtcattggc tgcgacatat
     5101 ccgcgctcat acatgcgtcg cccgatgtcg cacatctgaa cgcgcagcgt atcttcgctc
     5161 ggcgaatatt cataccggcc tagcgattcg gctaccgcgg aacgaagtat tttttgaaat
     5221 tttttattca taatcagttg accacaagtc ttgcgaatcg ccgtttgccg actttcaata
     5281 cgtcgccggt cgacggcaaa acctttgagt caagttcgga tattttttcg tcgttgatgc
     5341 ttaccgcgcc ctgctgtatc agacggcgtg cctcgccgtt ggactgcgcg aaaccggctt
     5401 gcgtcacaag ctcgataata tttatcggag tttttatgga aatttcaggc atatcggtcg
     5461 gcacgttttt ctgcgcgaaa acgcggtcga attcgtcact ggccgcctgt gcggctggca
     5521 aactataaaa attttccact atcatgcggc cgagacgagc cttagcctga cgcggatgcg
     5581 tcttatccgg gtcgcacagt gtcttgattt catccatcgg caggttcgtc agcagcgtaa
     5641 aatagttttc catgagcgag tcgggtatgc tcatgatttt tccgaacatg tcgttcggtt
     5701 cgtcggtcac gccgatgtag ttgcccttcg acttgctcat tttttccacg ccgtcaagcc
     5761 cgacgagtat gggcatgacc atgactatct gcggctgttg gccggcgttt atctgcaaat
     5821 cccgaccaac tagattattg aacgtctgat cagtgccgcc gagctcgaca tccgcctcaa
     5881 tcataacgga atcgtaaccc tgcatgagcg gatagaaaaa ttcgtgtacg cctatcggct
     5941 ggccggcttt gtagcgtttt tcaaaagtat cgcgttccat catctgcgct acggtcatct
     6001 gacttgccag gcggataacg tctgcaaagc tcagcttcgc cagccattcg ctgttatatc
     6061 tcacttcgag tttatctggc gacgtattca gaatcctgcc ggcctgctgg aaatacgttt
     6121 cggcattttt cttaatctgt tccggcgtca gcattggccg cgtcctgttt acgcccgtcg
     6181 ggtcgccgat gcgggcggtg taatcgccga ttatcatcac cgccttgtgg ccgaggtcct
     6241 gaaactgccg caacttgcga agcacgacag tatggccgag gtggatatcc ggcgacgtag
     6301 gatcaaggcc cagcttcacg cgcataggtt ttccgcttaa caggcggctg gcgagctcat
     6361 ctttggaata gaccgcctca gcgccgcgaa gcagaatatc aaggctggcg gcaacgctat
     6421 tatttgtcgt gtcattctgt gtcatggttc aaaaagcata ctatttcggc tggcgaatta
     6481 caacagtttc agaagttctt cagggcggtc tacaagtttc atcgccccgt atttcaacag
     6541 ttcctcggca gtacggaatc cccatgttgc gcctacggca aacattttcg cggcgacggc
     6601 ggttttcata tccgtattgg tatcgccgag gtaaagaaat ttttcccgcg gcaggcctat
     6661 ggttttcact atcatatcaa cgcctgccgg gtcaggtttg ggagctatgc catccatcac
     6721 gccctgcacg accacgaatt tgtcgatgtc gaaaaaacgc tctacgcaca gccttgtaaa
     6781 ctggtgcggc ttgttcgaca tgaccgacat ctttactccg cgtgccgcga gttcatcgag
     6841 aagttccgga atgccgctgt aaggccgcgt tttgacagcc cagccgttac cgtaaatttc
     6901 acgattacgc gcgacgattt tttttatcgt ctcttctgag atatcggaat cacagcaggc
     6961 acggcgagca agattgacca cgccgtcacc gacgtaatac ctgtaatttt ccagcggctg
     7021 cggcgcatag ccgaattcac ctattgtcgc attcatgcag tctgcaaggt cttcgagagt
     7081 atttagcaga gtgccgtcca ggtcaaaaac aacagcttca tatttcattt ttttctcgca
     7141 aatcttttta gaacctatcc caaaaacatg ggcgtcgccg aaatcgagca attttgcggg
//
LOCUS       DAONFI010000080         7195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_12095_length_7195_cov_3.756303, whole genome shotgun sequence.
ACCESSION   DAONFI010000080 DAONFI010000000
VERSION     DAONFI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12095_length_7195_cov_3.756303"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="PLK08_03810"
     CDS             <1..1525
                     /locus_tag="PLK08_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS52659.1"
                     /translation="DNGKPLRMLGIHQDIDEQKKIEDELILARTNAEDASKAKSIFLA
                     NMSHEIRTPMNAILGYTQLMQRDEGLTDVQRKYLEIINHSGTHLLDLINDILEMSKIE
                     AGRTRVNAVIFDFRSMLEDIEMMFHVKTSAQNVMLRTVIDEKIPRYIKSDENKIRQIL
                     INLIGNAVKFTSSGHIVISVNCGLQSRQPSEDNLGDIRFRIDVEDTGCGIAAGDMDKI
                     FEAFEQSRAAVESGGTGLGLPISREYARMLGGDITAKSEAGIGSTFTVEFVAQYVDEP
                     ERAAVEAKITKVTGLTAGRNSASVLVVDDRETNRALLVDLLDSVGFYVRHADNGLEAL
                     KIVQEWQPDVILMDRRMPVMDGYEAMRKIKDTPNGKNIPVLIVTASAFEENRTDAMGY
                     GAAGFIRKPYNEAEIFDAIRRATGIEYVYDKAETFRDIGNYQPSPQEMADLPERLRDN
                     MRQALHDGDMERMQIFIERVLEKNGMLGSYLASLAEHYEYAALQKLLSPEKPPACEK"
     gene            complement(1555..3804)
                     /gene="priA"
                     /locus_tag="PLK08_03815"
     CDS             complement(1555..3804)
                     /gene="priA"
                     /locus_tag="PLK08_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPS52660.1"
                     /translation="MNTLFSQQINESQITGHAVGIAVNANVWDSYTYLWPDSLGEPAF
                     GQRVRVPFGRGNRKITGFVIEPNQPAPADKILKTVTELIDHESLLTEDLKKLCLWINR
                     YYLTPLGMTLAAMLPTVVGTNSKKQQIFVTLQSQPTDWPSQLGKKQRTVLDELLEAKK
                     QGIDSLSLEELLHHSKASRQTVHSLAERNLISTETRPLNLEHLQNAPSSENDITLNDD
                     QQAVLEKFLPRLNNGFSVTLLRGVTGSGKTEIYVRAIRRVIEAGKQAILLAPEIALAT
                     QTLQRLLKRLPRVAVLHSGLSAAQRAFYWQQIRDGHAGVVVGPRSAVFAPIRPEKLGL
                     VVVDEEHESSYKQDTAPRYHGRDVAIMRASITGVPVLLGSATPSMESYHNAMNGRYAL
                     LELPNRVKGLPMPRLEIVNLRREMQPGRIELIGQTLQQRMAGVLDRGEQIILLMNRRG
                     YASYVFCPKCDWMLECDQCVRPMVWHQAIQLCVCHHCDKTAVLPERCPACGGKILLFG
                     YGIQRVEDELARKFPAARVARMDSDTMTSPNQFKKILDDFGSGNVDILLGTQMVAKGL
                     DFPKVSLVGVTSADTALALHDFRASERTFQLIVQVAGRAGRSEHPGQVVVQTLHEDEP
                     AIQYASQHDYKGFAGFELNDRSSMNLPPFSRMTRFMFRHEHANVANDAALKFAEFFRQ
                     KFPRNEVRIIGPQAAEMVKLQNMFRFDILLMTDKPMLVQNILFQEMGNLMRDVRADVF
                     ADVDPINLM"
     gene            complement(3842..4120)
                     /locus_tag="PLK08_03820"
     CDS             complement(3842..4120)
                     /locus_tag="PLK08_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52661.1"
                     /translation="MTKRQMIDEIMELNQSAQPEFLAMFDDEELDEYLSHLQWLTSPV
                     QMTGDTSQYDKYFPQERQIVQQQPLVTLWNTMEQDSAAEQQAAVAGLF"
     gene            4404..4477
                     /locus_tag="PLK08_03825"
     tRNA            4404..4477
                     /locus_tag="PLK08_03825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4438..4440,aa:Val,seq:tac)
     gene            complement(4893..5813)
                     /gene="rbsK"
                     /locus_tag="PLK08_03830"
     CDS             complement(4893..5813)
                     /gene="rbsK"
                     /locus_tag="PLK08_03830"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPS52662.1"
                     /translation="MTVAVLGSINMDMVTRVAAVPRPGQTVAGESFAMTPGGKGANQA
                     VAAARAGASVNFMGKVGGDLFASELISSLRTAGVNSDCVRVESKTSSGVAAITLTKDS
                     QNSIIVIPGANAMLVVQDVDTLAEFFPFSTCLLLQLEIPMDVNIKAARLAHQAGVRVI
                     LDPAPATVLPDELLVNVDIITPNQSEAKTITGLDANTYKQTNDVIARLVDMGIRQPII
                     KMGQHGVCWYEDGKIKSMKAFNVDVIDTVAAGDAFNGGLAAALDEHKSLDSAIMFAQA
                     TAAISVTRYGAQQAMPIKNEINAFLATRDK"
     gene            5825..6049
                     /locus_tag="PLK08_03835"
     CDS             5825..6049
                     /locus_tag="PLK08_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52663.1"
                     /translation="MYACNAAPHEVMLVEKTFDFCVTKYLPQKVVDGRAYDSDKCDNE
                     LVDERNVELIAPHKSNRIRPATQYGRKLRG"
     gene            complement(6229..>7195)
                     /locus_tag="PLK08_03840"
     CDS             complement(6229..>7195)
                     /locus_tag="PLK08_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPS52664.1"
                     /translation="VFWTIFEISAITANTVFTGLINDVVPHNVIGRFYGLFRGMSLLA
                     GIVFFYMFVGHAENYYMTIYIGLGAFYGIGFTAMCFNVKEGKYPSPPSSSDTSGMHPL
                     KAVKSYFYECFSSRYYLWYYCMIAMAFMSLMASFIFSVYAAQSFGLTLEDYGICLVLT
                     YTGSLLLSYPLGMLMDKFHPLRMCILTLFLHVLINLCGFIFIVDSLTFSIIFTVQGII
                     AGTYMTAYSGLSPMLLPRSRFSQIVSASTMVTGIGTILLGGLLGKFLDIIGHQYQYTF
                     GIACAFSVLALIASIYLYRGFISFGGSKNYIPPEPGKYKRQIILQ"
BASE COUNT         1872 a         1682 c         1777 g         1864 t
ORIGIN      
        1 cgataatggc aaaccgttac gcatgctcgg catacatcag gacatagacg aacagaaaaa
       61 aatcgaagac gaactgattt tagccagaac caacgccgag gacgccagca aggctaaaag
      121 tattttcctt gcaaacatgt cgcatgagat acgtacgccc atgaacgcca tactcggcta
      181 tacccagctt atgcaacgcg acgaaggcct taccgacgtt cagcggaaat atctcgaaat
      241 aatcaatcat agcggaacgc atctgcttga cctgataaat gatattctgg aaatgtccaa
      301 aatcgaggcc ggccgcacga gggtcaacgc cgtcattttc gatttcaggt caatgcttga
      361 agacatagaa atgatgttcc acgttaaaac gtcggcacag aacgtcatgt tacgaaccgt
      421 aatcgacgaa aaaattccgc ggtacataaa atccgacgaa aataagatac gtcagatatt
      481 aatcaatctc atcggcaatg cggtgaagtt tacgtcatcg ggccatatag ttatatctgt
      541 gaattgtggc ctccagagcc ggcagccgtc tgaagataat ttgggcgata tcagattcag
      601 gattgatgtt gaagataccg gttgcgggat agcggctggg gatatggata aaatttttga
      661 agcctttgag cagtcccgtg ctgctgtgga aagtggcggc acaggccttg gattgccgat
      721 aagtcgtgaa tatgcccgta tgcttggcgg cgatattacc gcaaaaagcg aagccggcat
      781 tggcagtaca tttaccgttg aatttgttgc gcaatatgtt gatgagcctg agcgggctgc
      841 ggttgaggcg aaaattacga aagttaccgg gctgactgcg gggagaaatt ccgccagcgt
      901 tctggtggtt gatgacagag agactaacag ggcgttgctg gtggatttgc ttgacagtgt
      961 gggtttttat gtgcggcatg ccgacaatgg cctcgaggcg ctcaaaattg tccaggaatg
     1021 gcagcctgat gtgatattga tggacagacg catgcctgtc atggacggct atgaagcaat
     1081 gcgtaaaata aaggatacac ccaatggaaa gaacataccg gtacttatcg ttactgcgag
     1141 cgcttttgaa gaaaaccgga ccgatgcgat gggatatggt gcggcgggtt ttatccgaaa
     1201 gccttacaac gaagcggaaa ttttcgatgc catacgccgc gctaccggca tagagtatgt
     1261 ttatgataaa gccgaaacat ttcgcgacat cggcaattat cagccttcgc cgcaggagat
     1321 ggctgatttg ccggaacgtc tgcgggataa catgagacag gcattgcacg acggcgatat
     1381 ggaacgtatg cagattttta tcgaacgtgt tttggaaaaa aacggaatgc tgggaagcta
     1441 tcttgcctcg ctggcggaac attacgaata tgccgcatta cagaaactgt tgtcgccgga
     1501 aaagccgccg gcctgtgaaa aataatctgc cgcagattga tgcgcatatg aactttacat
     1561 aagatttatt gggtctacgt cggcaaatac gtcggcacgc acatctcgca tcagattgcc
     1621 catttcctgg aaaagaatat tttgcaccag catgggtttg tcggtcatga ggaggatgtc
     1681 gaaacggaac atattttgaa gtttcaccat ttcagcagcc tgcgggccga tgattctcac
     1741 ttcattgcgt gggaattttt gacggaaaaa ttccgcaaat ttcagggctg cgtcgttagc
     1801 cacatttgca tgttcgtgcc ggaacatgaa tcgcgtcatc ctggaaaacg gcggcagatt
     1861 catgctgctg cggtcattaa gttcgaagcc ggcaaatccc ttgtagtcgt gctgtgaggc
     1921 atattggata gccggttcgt cttcatgcag ggtttgaacg acgacctggc ctgggtgttc
     1981 gcttcttccg gctcggccgg cgacctggac gataagttga aaagtccgtt ccgaagctcg
     2041 aaaatcgtgc aacgccagcg ccgtatccgc cgacgttacg cctacgagtg aaactttggg
     2101 gaaatccagg cctttggcga ccatctgtgt gcccagaagt atgtcaacgt ttccgctgcc
     2161 gaaatcatcc agaattttct tgaactgatt tggcgacgtc atggtatcgc tatccatcct
     2221 cgccaccctc gcagccggga atttcctggc gagctcgtct tcaactctct gtatgccgta
     2281 gccgaagagc aggattttcc cgccgcaggc aggacagcgt tccggaagaa ctgcggtttt
     2341 atcacaatgg tgacagacgc acaactgtat cgcctgatgc cagaccatcg gccgtacgca
     2401 ttggtcgcat tcgagcatcc agtcgcattt gggacagaat acgtaacttg cgtagcctcg
     2461 ccggttcatc agcaggatta tctgttcgcc gcggtccaga acgccggcca ttcgctgttg
     2521 cagtgtttgg ccgatgagtt caatgcggcc cggctgcatt tcccgtcgca gatttacgat
     2581 ttccagtcgc ggcattggca ggcctttgac gcgattcggc agctcgagca gggcataacg
     2641 gccattcatg gcattgtggt aggactccat ggacggcgtc gccgagccga gcagcaccgg
     2701 cacgcctgta attgatgccc gcatgattgc cacgtctctg ccgtggtatc gtggcgcagt
     2761 atcctgctta taacttgact catgttcttc gtccacgacc acgaggccga gtttttcggg
     2821 gcgtatcggc gcaaaaaccg cgctgcgcgg ccctacgact acgccggcat ggccgtcgcg
     2881 tatctgttgc cagtaaaatg ctctttgtgc tgcggaaagg ccgctgtgta aaacggctac
     2941 gcgcggcaga cgcttcagaa gacgttgcag cgtctgtgtt gcgagagcga tttccggcgc
     3001 aagcaggata gcctgtttgc cggcctctat tacgcggcga atcgcacgga cgtatatttc
     3061 agttttgccg ctgccggtga ctccgcgcaa aagcgtcacg ctgaaaccgt tattcagtcg
     3121 cggcagaaat ttttccagaa ccgcctgctg gtcgtcattg agagttatgt cgttttccga
     3181 tgatggtgca ttctggagat gttcaagatt cagcggccgt gtttccgtag atatgagatt
     3241 ccgttcggca aggctgtgaa cggtctgtcg gctggctttg ctgtggtgta aaagttcttc
     3301 cagtgaaagt gaatcgatgc cctgtttttt cgcttcaagg agttcatcca ggacggtgcg
     3361 ctgttttttg cccagctgtg acggccaatc cgtcggctga ctttggagtg ttacgaaaat
     3421 ctgttgtttt ttggaatttg tgcctaccac agtcggcagc atggcagcca gagtcatgcc
     3481 cagcggcgtg agataatatc tgtttatcca gaggcagagt tttttcaaat cttccgtcag
     3541 cagtgattcg tggtctatga gttctgtgac ggtcttgaga attttatcgg caggcgccgg
     3601 ctgatttggt tctatgacga atccggtaat tttgcgattt ccgcgtccga acggcactcg
     3661 cacccgttgg ccgaaagccg gctcgccaag cgagtctggc caaagatatg tataagaatc
     3721 ccacacgttg gcattgacgg caatgccgac cgcgtggcct gtgatttgcg attcattaat
     3781 ctgctggctg aaaagtgtat tcatcagccg ccatggtaac ggctgatgaa gtcaaatgca
     3841 atcagaacaa tcccgccaca gccgcctgct gctcagccgc cgaatcctgc tccatggtgt
     3901 tccaaagcgt caccagaggc tgttgctgaa caatctggcg ttcctgcgga aaatatttgt
     3961 cgtattgcga cgtgtcgccc gtcatctgta ccggcgaggt cagccactgg agatgcgaaa
     4021 gatattcgtc cagttcctca tcgtcgaaca tcgccaggaa ttccggctga gccgactgat
     4081 tcaattccat tatttcgtca atcatttggc gtttcgtcat tttagttttc tccggcaaaa
     4141 tccatttccc gttaagatga atatcggcca tttatgattc gaggcttgag tgtatttgcc
     4201 gtaagccggc gattagcgtt gccataaatc ggaagctgtt tcaaaacctt atttctgctg
     4261 cggctggcct gccattaaaa cgtttattgt tgcgacgaaa gcagattttg gaataatcag
     4321 atgtaacgag tttatttttt aagcgatttg tcaaaaatga cttgagttta tgagctgaat
     4381 atgtaaaatt cgcctttttg tccgggccgt tagctcagtt ggctagagcg cctgccttac
     4441 aagcaggagg tcgttggttc gagcccatca cggcccagtg tggtttctat ttgtcatgct
     4501 tgtctttgtg taattttccg attatcactt caaaatagtg aacttttacc ttcaggtaat
     4561 gcttcattgc tactgtctgt gagttttcta gaacttatcc caaaaacatg ggcgttgccg
     4621 aaatcgagca attttgcgag ctggcaagga ataaaaatgg cggcatattt ttttaaaaaa
     4681 atattttgcc gtttttctga cgccgccagg ctgcaaaagg gccgattgca ggcagccaag
     4741 gttttgggat agcttctaag ctgtaagact ccttttaggg gccttcctca taccaccagt
     4801 tctactggtg gttaatgatt gcgaataatt aagggataag tactttgaat tactactata
     4861 cgttattcct cggaagccag agtatagaat cttcatttat ctctcgtggc aaggaatgcg
     4921 tttatctcat ttttaatggg catggcttgc tgggcaccat aacgagttac agatattgca
     4981 gctgttgcct gcgcaaacat aattgctgaa tctaacgatt tatgctcatc caatgctgca
     5041 gccaaacctc cattgaaggc atcacctgcg gcaaccgtgt caataacatc aacattgaaa
     5101 gctttcattg atttgatttt tccatcctca taccagcaca cgccatgttg ccccatcttg
     5161 attataggtt gtctgattcc catatccaca agtcgggcta ttacgtcatt ggtctgctta
     5221 taagtattgg catccaatcc ggtaatcgtt ttagcctcag actgattggg agttataata
     5281 tcaacattga caagaagctc atctggcaaa acggttgctg gagcaggatc aagaatcacg
     5341 cgtacaccag cctgatgagc tagtctggcg gctttgatat tgacatccat aggaatttca
     5401 agctgaagca gaaggcaagt ggagaatggg aaaaattctg caagcgtgtc cacatcctgc
     5461 acaaccaaca tggcgttggc accaggtatt acgataatgc tattttgaga gtctttcgtc
     5521 aaagttattg ccgcaacacc tgatgatgtt ttggattcaa cccgaacaca atctgagttt
     5581 acgccagccg ttcttagtga agagattaac tctgaagcaa aaagatctcc acctaccttc
     5641 cccatgaaat tcacagatgc cccagcccgt gcagctgcca cagcctgatt tgcccctttg
     5701 ccaccaggag tcatagcgaa tgactctcct gcaacggtct gcccaggccg aggtaccgca
     5761 gccaccctcg tgaccatatc catattaatg ctacctaaaa ctgcaacagt catatacaat
     5821 caccgtgtac gcatgcaacg ctgcgccgca tgaagtaatg cttgtggaaa agactttcga
     5881 cttctgtgtg acgaaatatt tgccgcagaa agttgttgac ggcagggcct acgactcgga
     5941 caagtgtgat aatgagctgg ttgatgaacg gaatgttgaa cttatcgcac cgcacaaatc
     6001 gaaccggatt aggcctgcta cgcagtacgg cagaaagctg cgaggctaaa aacgttgatg
     6061 gaagagcgaa cgattgttcg catggctgca gaattaccgc cgcgtcgttg tgcgttggga
     6121 cagaaaagtg tcgatttata aggcttttgt gcatgttgcg tgtattgtta ttttacttga
     6181 caggttttga aacagcttcg tacattgttt tattatggtg gttacgcttt attgcagaat
     6241 tatctggcgt ttatacttgc caggctcagg aggaatataa ttcttgctgc cgccaaaaga
     6301 tataaatcca cgataaaggt atatactggc tatcagtgcc aacacgctga acgcgcaggc
     6361 tatgccaaac gtatactgat attgatggcc tatgatatca agaaatttgc ccaataaacc
     6421 acccaatagt atggtaccaa tacctgttac catcgtgctg gcagatacta tttgcgaaaa
     6481 acgcgacctc ggcagaagca tgggcgacaa accggaatat gctgtcatat acgtaccagc
     6541 aattattccc tgaacggtaa aaataattga aaacgttaat gaatcgacta taaaaatgaa
     6601 gccgcaaaga tttatcagta catgcaaaaa caacgttaaa atgcacatgc gcaaaggatg
     6661 aaacttatcc atcagcatac cgagcggata ggacaatagc agcgaaccgg tataagtcag
     6721 taccagacag atgccgtaat cttccaatgt caaaccaaaa ctctgggccg catatacgct
     6781 gaaaataaac gacgccatta gtgacataaa cgccatcgct atcatacagt aataccacag
     6841 gtaatatcgc gacgagaaac attcgtaaaa atatgattta acggctttta atggatgcat
     6901 gccactagta tcggacgatg atggaggaga aggatatttg ccttctttga cattgaaaca
     6961 catcgccgta aaacctatgc cgtaaaaagc ccccaatcct atatatatcg tcatataata
     7021 attttcggca tgcccaacga acatgtaaaa gaatactata ccggcaagaa ggctcatacc
     7081 cctgaaaagg ccgtagaacc gccctataac attatgcgga acaacgtcgt ttatcaaacc
     7141 ggtgaataca gtattggctg ttatcgctga aatttcgaaa atcgtccaga aaacg
//
LOCUS       DAONFI010000081         7153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_12194_length_7153_cov_4.148633, whole genome shotgun sequence.
ACCESSION   DAONFI010000081 DAONFI010000000
VERSION     DAONFI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12194_length_7153_cov_4.148633"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLK08_03845"
     CDS             complement(<1..664)
                     /locus_tag="PLK08_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52665.1"
                     /translation="MTHFKPSKPTLLSAFIVLSAALTFLGPDSAGALRARLHWMLAPL
                     GDAGMAVTTAFKNLTTPSEPIITQRYAQQLKQENVLLRQQLESMEARLLRVLGTLQDG
                     QAEFSHLFGPRKDVPVEFIAARVVAADSLPYGWSRVLNEGRDQGIRNGMYATQLRVIH
                     DRSKQITGNPMVISGATVVGRVIESGAFTSRLALLTDGGFEMYGQIRRVIDPRNPRMI
                     RYR"
     gene            complement(661..1074)
                     /locus_tag="PLK08_03850"
     CDS             complement(661..1074)
                     /locus_tag="PLK08_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52666.1"
                     /translation="MNETSNNYYFTVIVLLVVMLVAMAGLWVMEHKKLNRAVAFIRIQ
                     QNEKQTQDTRLRDMLLNATVEKNHMLDRDSFVKKGVTINGRLADVFVVTADDAAKAGL
                     LDGDIIFVGKTFTPSTQPSPAPKISKTAEIKNTGQ"
     gene            complement(1076..2503)
                     /locus_tag="PLK08_03855"
     CDS             complement(1076..2503)
                     /locus_tag="PLK08_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52667.1"
                     /translation="MNNQNKNWKIRFINPRSPLSTITMPGIIQKMTFSRKSVFAPLNL
                     TICAAVVPSNWQPEIVDENILEQPHTPQADVDAVGIGAMTSQAPRAYELADAYRRLGV
                     KVILGGIHPSALPDEALTHADVVCRGDAEGTLPYALNDIAAGGQKQIYDWQEHPDAPI
                     ATPRKDLLNPKDYLAFNPIQTTRGCPHGCTFCTTPAIFGRKYRQRDIGDIVEEIRAAK
                     EQFNSRVFIFSDDNVAGNQKWAMELFEAITPLKIRWASQCDILISRNDALLKAMRRSG
                     CEGLIIGLESPKAGTLKEAGKRFISADEYIDRIHKIQSFGMSLWGSFIFGFDSDGWRD
                     CMNAVRFAQHAKLCMSCYIILTPYPGTAVFDEFRRSGRLITEDWAKYNGSTVCFQPQK
                     MTVNELRHAQMAAFYEFYRMPSAIKRLGFRPLKKNSWMANLAIQRGLDYYYSKKGRPT
                     PSFSDFAAPDAQRRIERSLGLLRGL"
     gene            complement(2563..3051)
                     /locus_tag="PLK08_03860"
     CDS             complement(2563..3051)
                     /locus_tag="PLK08_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPS52668.1"
                     /translation="MQTIKIMSFTVILACIAAGCDPNFDYNGQRAQPGAMAVQSEWNV
                     TGTPGQFSFLSFAHDGNAMTVCQAKTYNRGQYITIDFTRPCIFNMIAIQHGPFEYGYA
                     REIAVEISNDGKNFTRIFTGEGTRKITYLCILSPVKARYVRLVALRPGIEKWSIAEVY
                     FQ"
     gene            3154..4395
                     /locus_tag="PLK08_03865"
     CDS             3154..4395
                     /locus_tag="PLK08_03865"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPS52669.1"
                     /translation="MENKAIIISIGNELTSGQGLDTNSAWLSRRLAEKGVWVISHHTV
                     GDDLAAISSAIVQAAEKVGLVIISGGLGPTADDLTRFALADAMGVKLVLDEASLRQIE
                     HMFESWGRAMSQSNRIQAMMPDGATAMENTQGTAPGIKATLGGTAIYVTPGVPREMKV
                     MFDKCILPELAVCDDVILHEIVNLFGAGESVIGEKIQDIMQKTGPVIVGTTVANGLVS
                     VRIISSAASRAESQKQIDAAVAELRRRLGKLVVGVGSEASMSAVVGELLEKSGMTVAT
                     AESCTGGLIGKMLTDRAGSSKYYVGGIISYSNEVKQEQLGVAQKLLDRYGAVSEQVAA
                     AMAVGVRERLHCDWGISITGIAGPGGGTDEKPVGLVYTALAGHDGVEVAKNIIPPHSR
                     ESVRLRSALTALDALRLKLLK"
     gene            complement(4535..5596)
                     /locus_tag="PLK08_03870"
     CDS             complement(4535..5596)
                     /locus_tag="PLK08_03870"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HPS52670.1"
                     /translation="MMISDLSNRASEWLCGIGPMHDVVVSSRVRLARNLAGMPFLSKC
                     DARQKSEILEKLRIAIMACDLGEKLTYVDVARATPLDRELLTERHLISRQHAASDSPR
                     GVIISDNETVSLMINEEDHLRMQVLRSGLQLNEAFDEIFRIDAALEKVLNYAFSSRYG
                     YLTACPTNVGTGLRVSVMLHLPALKLTGELDRALRAARDMKLAVRGLYGEGTDSLGDF
                     FQISNQITLGRSEKDIVEEFLSVIVPELLNYERKARQALMAHDATDIEDKIFRALAIL
                     QSARKITSKETMYRLSMIRLGVHLGKITSFDLKAVNELSLLAQPAHLQKILGREIQDE
                     ERQKVRADFLRQRLKACSS"
     gene            complement(5593..6141)
                     /locus_tag="PLK08_03875"
     CDS             complement(5593..6141)
                     /locus_tag="PLK08_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPS52671.1"
                     /translation="MMSDDRKCDKCGKPATVHLTEIVDGKKLEKHLCEECAEAEGITI
                     KANVPIGQLLENIIQHAQETASGEMSQNAAIASAPANEDVICNVCGMSFAEYNEHGLL
                     GCPNDYDAFGEKLAKAILHVQDGSDRHVGKIPANADVMLTLQNRILKLRSDLRQAIHA
                     EQYEHAAQLRDTIKAIEEQQAK"
     gene            6251..>7153
                     /gene="miaA"
                     /locus_tag="PLK08_03880"
     CDS             6251..>7153
                     /gene="miaA"
                     /locus_tag="PLK08_03880"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPS52672.1"
                     /translation="MNTDKVYFILGCTACGKAAVGRELAMRLNARILSVDSMKIYRRM
                     NIGTATPSAETQKLIRHYAVDVVEPCDSYSVAQFVQCADAAVDECRRDGKICLAVGGT
                     SLYIKALTEGLFEGPSADENIRNELRATAAGLGGIEELYRRLCAADPESAERIHRNDE
                     KRVIRALEVFIQTGVPISRLQKQWDAGRKRLDCTLIGLRRDKDVQSRRINARVRKMVD
                     MGLRDEVVSLLAEEKPLSPQAAQAVGYAEVIDHLAGKYDWEHAVEQIKINTRRLAKKQ
                     RTWHRRWRDVVWFDMDEDEPAQVTT"
BASE COUNT         1483 a         1804 c         2090 g         1776 t
ORIGIN      
        1 cgcggtaacg aatcatgcgc ggattgcgag ggtcgattac gcggcgtatc tggccgtaca
       61 tttcgaatcc gccgtcagtc agcaacgcca gccgcgatgt gaatgcgccc gattctatga
      121 ctctgccgac gaccgtcgcg ccggatatca ccatcggatt gccggttatc tgtttcgagc
      181 ggtcgtgtat cacacggagt tgggtggcgt acatgccatt gcggatgccc tggtcacggc
      241 cttcgttgag cactcgcgac cagccgtaag gcagcgaatc ggcggctacg acccgtgcgg
      301 cgatgaattc aacgggtacg tcttttctcg gcccgaaaag gtggctgaat tcggcttggc
      361 cgtcctggag cgtgcccagc actcgcaaca gccttgcctc catcgattca agctgctgac
      421 gaagaagcac gttttcctgc ttcaactgct gtgcgtatcg ctgcgtgatt atcggttcgg
      481 aaggggtggt gagatttttg aacgcggttg tgacggccat tcctgcgtcg cccagcggcg
      541 cgagcatcca gtgcagcctg gcacgcaatg cgccggcgga atcggggccg agaaacgtca
      601 atgccgccga cagcactatg aacgccgaca acagcgtcgg cttggatggt ttgaaatgcg
      661 tcattgtccg gtgtttttaa tctccgctgt tttcgatatt tttggggccg gcgacggctg
      721 tgttgacggc gtaaatgttt tgccgacgaa aattatgtcg ccgtccagca ggccggcttt
      781 tgcggcgtca tcggcggtta cgacaaaaac atcggccagc ctgccgttta tcgtcacacc
      841 ctttttgacg aaactgtcgc ggtcgagcat gtgatttttt tccactgtcg cattcaggag
      901 catatctcgc agtctggtgt cctgcgtctg tttttcgttc tgttgaatgc gtatgaacgc
      961 cactgctctg ttgagttttt tatgttccat gacccatagg ccagccattg cgacaagcat
     1021 gacaaccagc agtactatga ccgtgaaata ataattgttc gatgtttcat tcatgtcaga
     1081 gtcctcgcag aaggccgagg ctgcgttcta tccgccgctg tgcgtccggt gcggcgaaat
     1141 cgctgaatga aggcgtcggc ctgccttttt tggaatagta ataatccagt ccgcgctgga
     1201 tggcgagatt ggccatccac gagttctttt tcagcggccg gaagcccagt cgttttattg
     1261 cgctgggcat tctgtaaaat tcgtaaaaag ccgccatctg cgcatgacgc aattcgttga
     1321 ccgtcatttt ttgcggctga aagcatacgg ttgagccgtt gtatttcgcc caatcctccg
     1381 tgatgagacg ccccgaccgg cgaaattcat cgaataccgc cgtgccagga tagggcgtaa
     1441 gtattatgta acaactcatg cagagcttcg catgctgggc gaatcgcacg gcgttcatgc
     1501 agtcacgcca gccgtcggag tcgaaaccga aaatgaaact gccccagagg ctcatgccga
     1561 aggactggat tttatgaatg cggtcgatgt attcgtcggc ggagatgaat cgtttgccgg
     1621 cttctttgag agtgccggct tttgggcttt cgaggccaat tatgaggcct tcgcagccgc
     1681 tgcgacgcat ggctttcagc agggcgtcgt tgcgggatat gagtatgtca cactggctgg
     1741 cccagcggat ttttaggggc gttatggctt caaaaagctc catggcccac ttctgattgc
     1801 ctgcgacgtt gtcgtcggag aatatgaaca cgcgcgagtt gaactgctct tttgcggcgc
     1861 ggatttcctc cacgatatcg ccgatgtccc gctggcggta tttgcggccg aagatggccg
     1921 gcgtggtgca gaacgtacag ccgtgcggac agccccgcgt ggtttggatg ggattgaacg
     1981 ccagataatc tttcgggttg agcaggtcct tgcgcggcgt ggcgattggg gcgtcgggat
     2041 gttcctgcca gtcgtaaatc tgtttttggc cgccggctgc gatgtcgtta agcgcgtagg
     2101 gcagtgtgcc ttcggcatct ccgcggcaga ctacgtcggc atgggtgaga gcttcatcgg
     2161 gcagggctga tgggtgtatg ccgcccagta tgacctttac gcccagcctg cgatatgcat
     2221 ccgccagctc atatgccctc ggcgcctgac ttgtcatcgc ccctatcccg accgcgtcga
     2281 cgtctgcctg cggcgtatgg ggctgctcga gtatgttttc atcgactatt tccggctgcc
     2341 agttcgacgg aactaccgct gcgcatatgg tcaggttaag cggagcgaaa acgcttttgc
     2401 gggaaaaggt catcttctga atgattcccg gcatggtgat tgtgctcagc gggctgcgcg
     2461 ggtttatgaa ccggattttc cagtttttat tttgattgtt catggcgtct gtgtcagccg
     2521 ctgttgcgaa ggtctctctg gctgtcgcga ttggcggtgg tgttactgga aataaacttc
     2581 ggcgatggac catttttcaa tgcccggccg caacgcaacc agccgtacgt agcgagcctt
     2641 taccggcgaa agtatgcata gataggttat ctttcttgtg ccttcgccgg tgaatattct
     2701 tgtaaaattc ttgccgtcgt tacttatttc gacggcgatt tctcttgcgt agccgtattc
     2761 gaacggcccg tgctgtatgg ctatcatgtt gaaaatgcac ggccgggtga agtcaatggt
     2821 tatatactgg ccgcgattgt aggtcttggc ctgacagaca gtcatggcat tgccgtcgtg
     2881 agcgaacgaa agaaaactga attggccggg tgtgccggtg acgttccatt ccgactgcac
     2941 agccatcgcg ccgggctgag cccgctggcc gttgtaatca aagttcgggt cgcagccggc
     3001 agctatgcac gccagaatta ccgtaaatga cattatcttt atcgtttgca tattgttcgc
     3061 tatcctattt gcaatctgtt tattatactt accggcagag gaagttaaag agtttttatg
     3121 ggtttcctgc ccgcaatata aggctgtaaa gaaatggaaa ataaggcgat aataatttca
     3181 atcggtaatg agttgacgtc cggccagggc ctggatacga attcggcgtg gctttcgcgt
     3241 cgtttggccg aaaaaggtgt ttgggtaata tcgcatcata cagtgggcga cgatcttgcg
     3301 gcgatatcgt ccgcgattgt tcaagccgca gaaaaggtcg gattggtgat aatttcaggc
     3361 ggccttgggc cgacggctga cgaccttacg cgattcgcac tggctgatgc catgggcgtg
     3421 aaactggtgc ttgacgaggc cagccttcgc cagatagagc acatgttcga atcctggggc
     3481 agggcgatgt cgcagtcgaa ccgcattcaa gccatgatgc ccgacggtgc gacggcgatg
     3541 gaaaataccc agggtacggc gcctggcata aaggcaacgc ttggcggcac ggcgatttac
     3601 gtgaccccag gcgtgccgcg agaaatgaaa gtcatgttcg ataaatgcat tctgcccgaa
     3661 ttagccgtct gcgacgatgt gatacttcac gagattgtaa atctcttcgg agccggcgaa
     3721 tcggtcatcg gcgagaaaat tcaggatatc atgcagaaga ccgggccggt gattgtcggc
     3781 acgacggtgg cgaacggcct ggtgtcggta cgtataatct cgtccgcagc cagccgcgct
     3841 gaatcgcaga aacagataga tgctgccgtt gccgaacttc gcaggcgact ggggaaactg
     3901 gtcgtaggcg tggggagcga agcatcgatg tcggcagttg tgggcgaatt gttggagaaa
     3961 agcggcatga cggttgctac ggctgagagc tgcacgggcg ggctcatcgg caaaatgctc
     4021 accgaccgcg ccggctcgag caaatattac gtcggcggga taatttcgta ttccaacgag
     4081 gtcaagcagg agcagctcgg cgttgctcag aaacttttgg acagatatgg cgcggtaagc
     4141 gagcaggtgg cggcggctat ggctgtgggc gtgcgagaac ggctgcattg cgactggggg
     4201 atttctatta ccggcatcgc cggccctgga ggcggcacgg atgaaaaacc ggtcggcctc
     4261 gtgtacaccg cgctagccgg ccatgatggc gtggaagtcg caaaaaatat cattccgccg
     4321 catagccgcg agtccgtccg gttacgcagc gcattgaccg cccttgacgc gttgcggctg
     4381 aaattactga aatagaaggt atcgggcgat tttttatggc gatttttgaa ataacgcaag
     4441 attaaaactt gcaacgagcc ctgttagccc tgttaataaa ggcccgtaca tcagaacatt
     4501 gcaagacttt aaagaaccgc tctataactg cgtctcaact tgagcatgct ttaagccgct
     4561 gccgcagaaa gtctgcacgt actttctgac gttcttcgtc ctggatttct ctacccagta
     4621 ttttttgcag atgtgccggc tgggccagta atgaaagttc gttgaccgct tttaaatcga
     4681 aactggttat tttccccaga tgcacgccca gccgtatcat tgatagccgg tacatggttt
     4741 ccttgctggt gatttttctg gctgattgca gtatagccag tgcgcggaag attttgtctt
     4801 cgatgtctgt ggcgtcgtgg gccatgagag cctggcgggc tttgcgttcg taattgagca
     4861 gttcgggcac gatgacggac aaaaattcct ccacgatgtc cttttcgctc ctgcccagcg
     4921 taatctggtt ggatatctgg aaaaaatcgc caagcgagtc cgtaccttcg ccgtagaggc
     4981 cgcggacagc cagcttcatg tcgcgggcgg cccgtagtgc acggtcgagt tcgccggtga
     5041 gtttcagggc cggcagatgc agcatgacgc ttacccgtaa acctgtgccg acgtttgtcg
     5101 gacaagccgt caaataacca tacctggatg aaaatgcata attcagcact ttttccaatg
     5161 ctgcgtctat acgaaagatt tcgtcgaacg cctcattcag ctgcaagccg ctgcgcaata
     5221 cctgcatgcg aagatggtct tcttcgttta tcatcagcga gacggtttcg ttgtcgctta
     5281 ttatgactcc gcgtggcgaa tcgctcgctg cgtgctgtct gcttatgagg tgacgttccg
     5341 taagcagttc gcggtcgaga ggtgttgctc gtgcgacgtc gacgtacgtg agtttttcgc
     5401 ccaggtcgca ggccattatg gcgatgcgga gtttttcgag tatttcgctt ttctggcggg
     5461 cgtcgcattt tgacagaaac ggcatgccgg cgaggtttct tgccagccgc acgcgggatg
     5521 aaaccaccac atcgtgcatc ggccctatgc cgcacagcca ttcactggcg cggttggaca
     5581 ggtcgcttat catcattttg cctgctgctc ctcgatggct tttatggtat cgcgcagttg
     5641 tgcggcgtgt tcgtactgtt cggcgtggat ggcctgtctc aaatcggaac gcaatttaag
     5701 tattctgttc tgaagcgtca gcatcacatc ggcgttagcc ggtatcttgc ccacatgcct
     5761 gtcactgccg tcctggacgt gcaggatagc ttttgcaagt ttttcgccga atgcgtcgta
     5821 gtcattggga cagccgagca ggccgtgttc gttatattcg gcaaaactca ttccgcacac
     5881 attgcatatg acgtcttcgt tggcgggggc ggatgcgatg gcggcattct gcgacatttc
     5941 gccggaggcg gtttcctggg catgctggat gatgttttca agaagctggc ctatcgggac
     6001 attagccttg atggtaatgc cttccgcctc ggcgcattct tcgcagagat gtttttccag
     6061 ctttttcccg tccacaatct cagtgaggtg tacggtcgcc ggtttgccgc atttatcgca
     6121 ttttctatcg tcgctcatca tttcttatgg tacttcaata ttagccggtt atcaagaaaa
     6181 tcaggctgaa tcgtgactgt atttgtggtt atgatttaca cagccgccgt gacccgctaa
     6241 aataccgccc atgaatacgg acaaggtata ttttatcctg gggtgtactg cgtgcggcaa
     6301 ggcagccgtc ggccgtgaac tggccatgcg gctgaacgcc agaatcctgt ctgttgattc
     6361 gatgaaaatc taccggcgga tgaatatcgg cacagccacc cccagcgctg aaacgcagaa
     6421 attaatcagg cattacgcag tcgacgtcgt tgagccatgc gactcgtaca gtgtcgccca
     6481 gtttgttcaa tgtgcggacg cggctgtcga cgagtgcagg cgcgacggga aaatatgtct
     6541 tgccgtcggc ggcacgagcc tttacataaa agcattgacg gaaggactct ttgaagggcc
     6601 atcggcggat gagaacatac gcaatgaact cagggcgacg gctgccgggt tgggcggcat
     6661 tgaagagctt tatcggcgat tgtgtgccgc tgaccctgaa tcggctgaaa gaattcacag
     6721 aaacgacgaa aagagggtca ttcgcgcgct ggaggttttt attcagaccg gtgtgccgat
     6781 atcgcggctt cagaagcagt gggacgccgg ccggaaacgg ctggactgca cgctcatagg
     6841 tttgcggcgg gataaggacg ttcagtcgcg acgcataaac gcccgcgtca ggaagatggt
     6901 cgacatgggt cttcgagatg aggtcgtctc tttgctcgcc gaggaaaagc cgctcagtcc
     6961 gcaggctgca caggctgtag gctatgccga agttatagac catcttgcag gtaaatacga
     7021 ctgggaacat gccgtcgaac agataaagat aaacacgcga cggctggcta aaaaacagcg
     7081 cacgtggcat cgtcggtggc gggatgtcgt atggttcgat atggatgaag atgaaccggc
     7141 tcaggttacg acg
//
LOCUS       DAONFI010000082         7091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_12343_length_7091_cov_4.618107, whole genome shotgun sequence.
ACCESSION   DAONFI010000082 DAONFI010000000
VERSION     DAONFI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12343_length_7091_cov_4.618107"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLK08_03885"
     CDS             <1..180
                     /locus_tag="PLK08_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52673.1"
                     /translation="SLAVKSGGTAAFRMFFQPCLSRSDIPEEVLFQAVTNPGGVAIAA
                     TSREVAFVGMPVFFR"
     gene            193..1368
                     /locus_tag="PLK08_03890"
     CDS             193..1368
                     /locus_tag="PLK08_03890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPS52674.1"
                     /translation="MLIPVRTDYRLKKIPWVNYTIIALNVALYLMGVNGASRHGMTLI
                     GPYMLDPHSPALVQFFTSVFLHANLGHLLGNMLFLWVFGNAINDKFGPVGYLAFYLGG
                     GVLAGAGYLMLAGNAPVLGASGAIAAVCGAYLVLLPRTNVTFILWMFLIIPLSVPSLY
                     AIAFQFIFEIIMTIAGWSGPAAGGVAYAAHASGYLFGIIVAMLLLRLGMIERDDFDML
                     HLFQQHRRRQAFLREVLNGRDPFGRQAPVAMRVTQTQPADTKSAAELDLRKRISAGVR
                     CGDYPGAAAFYTKLLVEDSSAVLPRQQQLDIANQLMASRAYEAAAAAYELFVNAYGDY
                     EYMGDIYLMLGLLYCRYLHNEERARRNLALAMEKLQDESKLHKARQCLEELDRRYMP"
     gene            1433..2194
                     /locus_tag="PLK08_03895"
     CDS             1433..2194
                     /locus_tag="PLK08_03895"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HPS52675.1"
                     /translation="MQQVHNIPASMPTQEQARQSLDRLGELVSSLFSGQGEAWEKHLS
                     AVADLQYVECSLIFVAGLVYMIWGWKIFKPLVILNIALVGGVLGGMAAVSLKNEPYWW
                     AGMIIGAIVFGVAAWPLFRFCLALIGMAFGSMVGYAFYTELIVFMNRPDMANYPWLGA
                     VAGFIIFGVIAIGFVKPAIILLTSLQGAMMILAASLGLLCKASSLRPELLDRLGTSPW
                     IAQAAVAVLTLVGIIIQITRSHKAADAAENGKKEA"
     gene            2191..2871
                     /locus_tag="PLK08_03900"
     CDS             2191..2871
                     /locus_tag="PLK08_03900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPS52676.1"
                     /translation="MSISENDILKILGAVAEKRESMEAALAGIDEHVSAEVRRLAAMF
                     DELRVQADTDYLTGANNRRYFFRRTDEILRQADEHDGFRAAMLLYDLDNFKLYNDRYG
                     HGVGDEILCDTARLITSICRTQDVVARVGGDEFAVLFWETQPRRENSRPLSEAFELAD
                     RFRKAIMTLKPASLGECAQGRLTISGGLACYPQHGTNCAELLRSADDALLAAKRTGKN
                     TIVICGEF"
     gene            2872..3129
                     /locus_tag="PLK08_03905"
     CDS             2872..3129
                     /locus_tag="PLK08_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPS52677.1"
                     /translation="MTAGREKNRKELPGLRISQAAKNAGVSRQTVEYYILVGLVKPIR
                     GKDGRARFFDDKLVRRIKLIKELNATGYTLLDIRELFLKGK"
     gene            complement(3602..3859)
                     /locus_tag="PLK08_03910"
     CDS             complement(3602..3859)
                     /locus_tag="PLK08_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52678.1"
                     /translation="MPRRQRPPLNGAPYCGNKLTREVHNLDNEKPQCQIDRIIKTDNA
                     VPYHSLLLAQIDNYRLCKHCIGEPAQKDSSGIACNTQTDCP"
     gene            4168..4668
                     /gene="tpx"
                     /locus_tag="PLK08_03915"
     CDS             4168..4668
                     /gene="tpx"
                     /locus_tag="PLK08_03915"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPS52679.1"
                     /translation="MQVKMMGNDMMLEGQIPKLGEVMPSAKLLDNELKEVSTDSFRGK
                     VLLIATVPSLDTPVCDAEAHRFNKEAASLGSDIQILVVSMDLPFAQARWCGAAGIDKV
                     KTLSDHRYADFGEKFGVLIPALRLLARAIFVADRDGRLIYMQIVPEVTNHPNYDGALD
                     AVKKLI"
     gene            4974..5501
                     /locus_tag="PLK08_03920"
     CDS             4974..5501
                     /locus_tag="PLK08_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPS52680.1"
                     /translation="MLTQKLNDAINDQINAEMYSAYLYLSMAAWFESENFPGFANWMR
                     VQVQEETFHAMRFFDYVNSRNGRVILSPIDGPPTSWESVADVFKATLEHEEFVTSRIN
                     KLMDLAIAESDHALKSHLQWFVDEQVEEESNANTILGKLKLAQGAPQMLLMLDNEMAT
                     RVYTPPVTPETAAAQ"
     gene            5551..6756
                     /locus_tag="PLK08_03925"
     CDS             5551..6756
                     /locus_tag="PLK08_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPS52681.1"
                     /translation="MSCNSEVFSAVKVADKIYWVGAVDWSVREFHGYETTRGTTYNAY
                     LILADKITLVDTVKSGFRDEMFARIASVIDPQKIDVIISNHAEPDHSGELAATIHCIQ
                     PDVTYASAAGQKTLDLYYGIGDKITVVKEGQVVDLGGLSVVFADTKMCHWPDSMVSYC
                     PERKILFSQDGFGMHLASAERFADEVDQGVLRYEAAKYYANILLPLSGFVQKTVQKVT
                     DLKLDIDIICPDHGPIWRKDPMQIIQWYSQWADQKRNRKVVVLYDTMWKSTEKMAKAV
                     VEGVIAGGAKPTLMSAQAVHRSDVATRLLESGGFIIGASTLNNEILPRMADMITYVKG
                     LRPKGLVGGAFGSYGWSGESPTKLNAILEEMGVSIPAGPVKAKYAPSDEALQECFSLG
                     KAVAEKLNE"
     gene            complement(6856..>7091)
                     /locus_tag="PLK08_03930"
     CDS             complement(6856..>7091)
                     /locus_tag="PLK08_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52682.1"
                     /translation="YPLRQLQWTLVLADGRTMCGVIKGQPLYVYPVNGGDFQTVILAD
                     RQKGNIGQKLKELIYPKTIIFSRSAITIKKADR"
BASE COUNT         1683 a         1708 c         1914 g         1786 t
ORIGIN      
        1 tcactcgccg tcaagtccgg cggtaccgcc gcgttcagga tgttctttca accttgtctg
       61 tcgcgttccg acattcctga agaagtcctt tttcaggcag ttaccaatcc tggcggcgtg
      121 gcgattgcgg cgacttccag ggaagtggcg ttcgtcggta tgcctgtctt ttttagatga
      181 tttcactcaa caatgctgat acccgtcaga acagattatc gccttaaaaa aattccgtgg
      241 gtgaattaca ctataattgc actgaatgtg gcgctctatc ttatgggtgt aaacggcgcc
      301 agccgccacg gcatgacgct tatcggccca tacatgctcg acccgcattc gcctgcgctt
      361 gtgcagtttt tcacgagtgt ttttctccac gcgaatcttg gccatctgct gggcaatatg
      421 ctttttctgt gggttttcgg caatgccatc aacgataaat tcgggccggt gggttatctg
      481 gcgttttatc tgggcggcgg agtgttagcc ggcgcgggat acctcatgtt agccggcaac
      541 gcaccggttc tgggagcaag cggagcgata gccgccgtct gcggagcgta ccttgtcctc
      601 ctgccgcgaa ccaacgtcac gttcatactg tggatgttcc tgataattcc tctgtccgtt
      661 cccagccttt acgccatagc ctttcagttc attttcgaga taataatgac cattgccggc
      721 tggtctggcc cggcagcagg cggagtggcg tatgcggccc atgccagcgg atatcttttt
      781 ggcattattg tggcgatgct tctgttgcgg ctgggcatga tagagcggga cgatttcgac
      841 atgctgcacc tttttcagca gcatcgtcgc cgccaggcgt ttttacgcga ggtactcaac
      901 ggccgcgacc cgtttggccg acaggctccg gttgccatgc gagtcacgca aacacagccg
      961 gctgacacga aaagtgcggc tgaactggac ttgcgaaaac ggatatcagc cggcgtccgc
     1021 tgtggtgatt atcctggtgc agccgctttt tataccaaac tgctggtgga ggactcctct
     1081 gccgtactgc cgcgacagca gcagctggac atagccaatc agttgatggc gtcgcgggca
     1141 tatgaggcgg ctgcggcggc atatgagctt ttcgtgaatg cttatggcga ttacgaatat
     1201 atgggggata tatacctcat gctgggcctg ctttattgcc gatatctaca taatgaagag
     1261 cgggccagga gaaatctggc ccttgccatg gaaaaattgc aggacgagtc caagctgcac
     1321 aaggcccggc aatgtctgga agagcttgac cggcgatata tgccctgacg gcggggactc
     1381 acttgagcat aggacggctc gctagcatat ggaccaggaa acactcaatt cggtgcagca
     1441 agtacataat atccccgctt ccatgcccac gcaggagcag gccagacagt cgcttgacag
     1501 attgggcgag cttgtttcgt cgctcttttc cggccagggc gaggcgtggg aaaaacatct
     1561 ctccgctgtc gcagaccttc aatacgtcga atgcagcctg atattcgtag ccggccttgt
     1621 gtacatgata tggggctgga aaattttcaa gccgcttgtg atactgaata tcgctcttgt
     1681 cggcggcgtg ctgggcggca tggcggcggt gtcgctgaaa aacgaaccct attggtgggc
     1741 gggcatgata atcggcgcaa tcgtgtttgg tgtggcggcg tggccgctgt tcaggttctg
     1801 cctggcgctt atcggcatgg ctttcggctc gatggtgggc tatgcgtttt atactgagtt
     1861 gatagtattc atgaataggc ccgatatggc gaattatccg tggctgggcg cggtggccgg
     1921 gttcataatt ttcggcgtga tagctatcgg cttcgtcaag ccggctataa ttctgctcac
     1981 ctcactgcag ggagccatga tgatattggc tgcctcgctc ggcttgctgt gcaaggcgtc
     2041 ttcgcttcgg ccggaacttc tggaccgact tggcacgtcg ccgtggatag cgcaggctgc
     2101 ggtagccgta cttacgcttg tgggaatcat catacagata accaggtcgc ataaggcggc
     2161 tgatgccgcg gagaacggca aaaaagaggc atgagcatat cggaaaacga catactgaaa
     2221 atcctggggg ctgtcgccga aaagagagag tcgatggagg cggctcttgc ggggattgac
     2281 gaacatgtgt cagcggaagt ccgcaggctg gctgcgatgt tcgacgaact gcgagtacag
     2341 gctgataccg attatctcac aggtgcgaat aacaggcggt actttttccg gcggacggac
     2401 gagatactca ggcaggctga cgaacatgac ggattccggg cggcgatgct tctgtacgac
     2461 ctggataatt tcaaattata taacgatagg tatggccatg gcgtcggcga tgaaatactc
     2521 tgcgacacgg cacggctgat aacttccata tgcaggacgc aggacgtagt agcccgcgtc
     2581 ggcggtgacg agttcgcagt gttattctgg gagacgcaac cgcggcgtga gaattccagg
     2641 ccgctcagcg aggcttttga acttgccgac cgtttcagaa aggcgataat gacacttaag
     2701 ccggcatcgc tgggcgaatg cgcacagggc cgattgacca tttctggcgg cttggcgtgc
     2761 tatcctcaac atggaacgaa ttgcgccgaa ctgctcaggt cggcagacga cgcacttctt
     2821 gccgccaaac gaaccggaaa aaatacaatc gtcatttgcg gtgaatttta aatgacagcc
     2881 ggacgggaaa aaaacaggaa agaattgccg ggccttcgca tatcgcaggc tgctaaaaat
     2941 gccggcgtca gcaggcagac agtcgaatat tacatcctgg tgggccttgt gaaaccaata
     3001 cgcggcaagg acggcagagc cagatttttc gacgacaaac ttgttcgccg cataaaactc
     3061 atcaaggaac tcaacgcaac cggctataca ctgctggaca tccgcgaact ttttctcaag
     3121 ggcaaataac tgtaacctgt cctcaaaatc ctggttgccc gtaatcggga tttgcggata
     3181 atacctggac attcactgat attttcaaaa gttttttttt aaaaagattt gaatggatgc
     3241 gcatgggtga atcccgcatt ttgcgagagt cacccaccct ccgaggcgca ttcccttacg
     3301 gtaatttaag agtttttaac aaaattaaca ctttagagca gttcaatctt gagagttcct
     3361 tttttgcttg tttgctttgt ccgtggctgc gtcagaaaaa cttcagaata ctgccaagta
     3421 tgcctcattt tccttccttg ccacgaacaa acccactggc gcaaaaatat gaactctcaa
     3481 tctttcaatg ctctagaatg ctctttcagc caaccgtgtc acggtactgc gtttctttta
     3541 tttttgttaa aaaatgctct aaatctacga caggctctct taccggctga tattgtcaca
     3601 atcatggaca gtcggtttgt gtattgcacg caatgcccga actgtctttt tgtgccggtt
     3661 ccccgataca atgtttgcaa agtctgtagt tgtcaatctg tgccagcagc agggaatgat
     3721 atggtacggc gttgtcggtc ttgattatcc tgtctatctg gcattgcggc ttttcattat
     3781 ccaggttgtg tacttccctg gtcaatttgt tgccgcaata cggtgctccg ttaagcggcg
     3841 gccgttgtcg tcttggcata gccgaggtcc tttccgtgaa tcaaattgat ttcaaatgca
     3901 aagtcgcatt aaaaccgctc tctgatacac gtcagccatc ctattacatt atgggtgaca
     3961 actatctgtc acccgtacaa agtttttttt cgcagtcgaa acatgcagtt ttgaagtttg
     4021 tggatttgat tcctttttaa aatacggatt cggcggcttt gaaaaaaaat tacggcggaa
     4081 ggaaatgatg tttttttaag agatagccgg cgcctaaaat atgaattgag cgttattgtg
     4141 accaactttg cttttaagga gacgaatatg caggtcaaaa tgatgggaaa cgatatgatg
     4201 ctggaaggcc agatacccaa acttggcgag gtaatgccgt cggctaagtt gttggacaac
     4261 gagcttaagg aagtatcgac ggatagtttt cgtggcaagg tgcttctgat agctactgtg
     4321 ccgtcgcttg atacgcctgt ctgcgatgcc gaggctcacc gcttcaacaa ggaagccgcc
     4381 tctctcggtt ctgacataca gatactcgtc gtcagcatgg atttgccctt tgcgcaggca
     4441 cgctggtgcg gagccgccgg aattgataag gtcaaaacac tttccgacca cagatatgcg
     4501 gatttcggcg aaaagtttgg cgttcttatt ccggcgttgc ggctgttggc tagagccatt
     4561 tttgtcgccg accgtgatgg acggctgata tatatgcaaa tcgtgccgga agttacaaat
     4621 catccgaatt acgacggggc cctggacgcc gtcaaaaaac ttatctgatt ttttatggga
     4681 attccttatt gttgcgcctg aaccgctata atgccatttt caaaaataaa tgcggcggca
     4741 tagttgcaaa tatccaggct gccagtaaga ggcctgcgaa aacggcactg cgaaacagta
     4801 accgcatctg gcccacaatt ctcactggca ggccgtcttg ccagtggcag ataatataaa
     4861 attggagatg atataaaatc tcagccgctt gattatccgg aatatcgtgc gtatcattgt
     4921 tgattgaaat gtaattaaag ttgaaaagtt aaccatcttt ggagattttt attatgttaa
     4981 cccagaaatt gaatgacgcg ataaacgacc agataaatgc cgaaatgtac agtgcatatc
     5041 tgtatctttc gatggctgcg tggtttgagt cggaaaattt ccctggcttt gccaactgga
     5101 tgcgtgttca ggttcaggag gagacttttc acgcgatgcg ttttttcgat tacgtcaaca
     5161 gccgtaatgg ccgtgtgatt ctcagcccga ttgatggccc gccgactagc tgggaatctg
     5221 tggctgacgt ttttaaggcc actctcgaac atgaagagtt cgtcaccagc cgcataaaca
     5281 aactcatgga tctggctatc gccgagtctg accatgcgct gaaaagccac ctgcaatggt
     5341 tcgttgacga gcaggtcgag gaagagtcga acgccaacac cattctcggc aaactcaagc
     5401 tcgcacaggg tgcgccgcag atgctgctga tgctcgacaa tgagatggct acccgcgtct
     5461 atacgccgcc ggtaacgcct gaaaccgcgg cggcacagtg atttttcaga cgactatttt
     5521 ttttacagaa cgacaggaga tttttcagcg atgagttgta acagtgaagt tttttccgcc
     5581 gtaaaggttg cagataaaat ttattgggtc ggagcggtgg actggagtgt gcgtgaattt
     5641 cacggctatg agacgactcg cgggactact tataacgcct acctcatatt agccgataaa
     5701 ataacgctgg tggataccgt gaaaagcggt ttccgtgacg agatgtttgc cagaatcgcg
     5761 tcggtgatag acccgcagaa aatcgacgtg ataatttcca atcatgccga gccggatcac
     5821 tccggcgaac tggctgccac gattcattgc attcagcccg atgtgactta tgcgtcggct
     5881 gccggccaga agacgctaga tttgtattac ggcataggcg ataaaatcac tgtcgtcaaa
     5941 gaaggccagg ttgtcgacct cggcgggttg agcgtggttt ttgctgatac gaaaatgtgc
     6001 cactggccgg acagcatggt cagctattgt ccggaacgaa agattctgtt cagccaggac
     6061 ggtttcggca tgcatctggc cagcgcggaa cgtttcgccg atgaggtcga ccagggcgtg
     6121 ctgcgctatg aagcggcgaa atattacgcg aacatacttc ttccgctgag cggattcgtc
     6181 cagaaaacgg ttcagaaagt caccgacctc aagctggaca tagatatcat ctgtccggac
     6241 cacgggccga tatggcgtaa agaccccatg cagattatcc agtggtactc gcaatgggct
     6301 gaccagaaac gaaacagaaa agtcgtcgta ctttatgaca ccatgtggaa gagcacggaa
     6361 aagatggcca aggcggtcgt tgaaggcgtc atcgccggcg gagcaaagcc gacgttaatg
     6421 agtgctcagg ctgtacatcg cagcgacgtt gccacgcggc tgctggaatc cggcggattc
     6481 atcatcggcg cgtctacgtt gaacaatgaa atactgccca ggatggctga catgattacc
     6541 tacgtcaagg gtttgcggcc caaaggcctt gtcggcggcg catttggctc atacggctgg
     6601 agcggcgaat cgccgacgaa gcttaacgca attcttgagg aaatgggcgt cagtattccg
     6661 gctggcccgg tgaaggcgaa atatgccccg tcagatgaag ccttgcagga atgtttcagc
     6721 ctcggcaaag ccgtcgccga aaaactcaat gaataaaaaa acagttatat cgcagaaaat
     6781 ggagcatgct tcaaatattg cattgcgcca tgtcgctgag tatagcgcga tgaacagcgg
     6841 tttttagagg caaagctacc tgtctgcttt tttaatcgtt attgcactgc gcgaaaatat
     6901 tatcgtcttt gggtagatga gttccttgag tttctgcccg atgttgcctt tctgcctgtc
     6961 agccaggatt acggtctgaa aatctccgcc gtttacggga taaacatata gcggctgacc
     7021 ttttatgacg ccgcacatcg tacggccgtc ggcgagtacg agcgtccact gtaattgtcg
     7081 caggggatag g
//
LOCUS       DAONFI010000083         7086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_12358_length_7086_cov_5.192007, whole genome shotgun sequence.
ACCESSION   DAONFI010000083 DAONFI010000000
VERSION     DAONFI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12358_length_7086_cov_5.192007"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /gene="dnaE"
                     /locus_tag="PLK08_03935"
     CDS             <1..1838
                     /gene="dnaE"
                     /locus_tag="PLK08_03935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPS52683.1"
                     /translation="RDIDPEQLPLDDPEVYELFCNGYTDGVFQFESDGMKNVLKQMQP
                     SKITDLIAANAMYRPGPMELIPTYCHRKNGTETVPDVHPLVDDILAETYGIMTYQEQV
                     MMVLNRLGKLPLNRALTLIKAISKKKEKVIAGEKPNFIVGCGENGIDEKHAEELFSLI
                     LKFAGYGFNKSHSTRYAIVAYQTAYFKVHYPQEFLAATLTFECGDTDKVVQYMAEARR
                     MGIKVLPPDINTCGVDFAVDGDAVRFGLSAVKGVGPTAVEEIVRARKEAGGKFHDLYH
                     FCEFADLRIVNRGAIESLIKCGAFDAMGATRSAMTAAADSAISFGSKAAADRKSGQMS
                     FFGSGGLLRGKSDERPTFPNVEPWSKTQLLKAEKETLGFYVSDHPLVEYGRELSGLSY
                     PPQISLGAIEPDEHNYRHDMRLRIGCMIQDVRTVLTKKDSRKMAMLTLEDTSGKLDAV
                     VFPNTYEQLAGLIVKDELVFIIGQLDRTRERPQIIVDEIHPMNSALQDFTAGMTLTMP
                     HNDKVAGESLLKILQNHKGHCEVRVKVRPSCRPDVMVLISLSSNLYVQPSRPLMKDLE
                     NFLDDQNLIELAPKPVPSGRQRKGFQRKSPQEQTGPVSEAVTRFN"
     gene            complement(1932..2876)
                     /gene="fmt"
                     /locus_tag="PLK08_03940"
     CDS             complement(1932..2876)
                     /gene="fmt"
                     /locus_tag="PLK08_03940"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPS52684.1"
                     /translation="MRIIFCGSGTFALPSFEAVADSRHDLVLAVTQPPRKSGRGGKIN
                     PTVIAQAAYRRGIDCLETQDINSTEALRILQSCNANVMVVAEYGQFIREAARKSVSRA
                     CFNLHGSILPALRGAAPVNRAVINGDAESGVTTFELVDKMDAGAVYRTEKVQITPNET
                     AGELREKLAAVGTKVVLATLDDIEAGIAQPVEQDASLATFAPKLQKSDGYVDFEQSVG
                     RVYDRIRGCSPWPGAAADFAKADGKTTRVIITRSEIASGQARGAAGTIDDEFCVATGD
                     GRLKILEIQPAGRKNMDFKSFVNGYRVLPGDRFLKISE"
     gene            complement(2943..4139)
                     /locus_tag="PLK08_03945"
     CDS             complement(2943..4139)
                     /locus_tag="PLK08_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPS52685.1"
                     /translation="MTRFLIFLFPAVMDITVGLLLFVNPMRLTQAGRSSTLVTAIASI
                     WSVSYLLANHFVGIWTNRRNVAWLIISSGVSMAILSGCFIIFSGVTAMFVLVFLTGVS
                     VALFFAPFQYFMKIIGGGDNDGVVRSTALYTFSWSMGIALGPFLSGYILQYHNWQWCM
                     AMTSLLSILVSVGIYLLKHHADADARTENDSLTIEPVIRPKNASYAKMPDLAVMNWIS
                     SGLGISVVILIRALFAKTAKVNNLSDFEAGIVLFLLSGSQALSGLSMIFSRTWMYRPV
                     PVCIYGLLGVAATAMFGVVHVVWGFYAAAVIFGIYSGSMFFYFVFHSLVHPSKSGRYV
                     SINEMVVGGCGIAGPLFGGVLADSVSMSLPYLASAVMIAGALVYQCVVYAKARNDIQP
                     ILKELS"
     gene            complement(4234..5454)
                     /gene="lpxB"
                     /locus_tag="PLK08_03950"
     CDS             complement(4234..5454)
                     /gene="lpxB"
                     /locus_tag="PLK08_03950"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPS52686.1"
                     /translation="MTADDKNISDISKSSKKNHEKASPVIFMSAAEASGDHHAAELIR
                     RLREKYPAAEFIGAAGPCMAAEGCEVIADLTHKASMLGGPVTGVFYYMKKVRQLCRAM
                     TAVKPDIFVPVDSPALNWHLAKQARRCGVPVMYYVAPQVWAWAPWRIKKVRRLTDHVA
                     CLLPFEENYFRSRGVAATYVGHPLFDQLPPQHSADECPDFAIPWTSGEWKIAFLPGSR
                     PSEIRLHAKMMSRIADGIRRTYPLARCKFTAVGPREADMIKKYSRRDDLDIAVARTPE
                     VLAESHYAVAASGTVTLEVAHFGVPMAVVYHIGLPERILYKLFIRHLIRTQYLSLVNI
                     IAQKQLVPEFMPVGPDVKSITACVLDALGDYGYLLETRRAMLELTAGLCTANPRGANA
                     NIADIISTILERYS"
     gene            complement(5451..5852)
                     /gene="acpS"
                     /locus_tag="PLK08_03955"
     CDS             complement(5451..5852)
                     /gene="acpS"
                     /locus_tag="PLK08_03955"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPS52687.1"
                     /translation="MSETVLAHGIDLVDVPRLSQSIERFGEKFLDRVFTEFEQQYCDS
                     RQKSAMVSYAGRFAVKEAVLKVLGTGWRDGIAWTDVEVRNLPSGQPVVKLYNKCDEIA
                     RELGIEKIFISISHVDSAAVGSAVAVGRAAK"
     gene            complement(5845..6117)
                     /locus_tag="PLK08_03960"
     CDS             complement(5845..6117)
                     /locus_tag="PLK08_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52688.1"
                     /translation="MDEFSKIDKLVELAEELGMEVRRAPSLFGDEHPGGSLVRLKGRE
                     ILFLDPDASVEDQINVAVHALAGRESLRDRFLEPEIRQLLEEAGDE"
     gene            6615..>7086
                     /locus_tag="PLK08_03965"
     CDS             6615..>7086
                     /locus_tag="PLK08_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPS52689.1"
                     /translation="MNMTEVGTVKTGLLAVLLSAVFAGSAFCQSEYPFEGEVTGTDVY
                     VRSAPDMASYAVAKISKPARVTVTAKVSEGWYAIKPIQGCYAVVDQAFVKADTSEARP
                     SRATGVIVGDNVRIRAAGTLRRSNFDSILTMADKGEAVTIVGTVEEGDSTWYIIL"
BASE COUNT         1776 a         1934 c         1803 g         1573 t
ORIGIN      
        1 gcagggatat cgaccccgaa cagctgccgc tcgacgaccc ggaagtttac gaactgttct
       61 gcaacggtta taccgacggc gtattccagt ttgaatcgga cgggatgaaa aacgtcctca
      121 agcagatgca gccgagcaag ataaccgacc tcatcgccgc aaacgccatg tatcggcccg
      181 gccctatgga actgatacca acgtactgcc atcgtaaaaa cggcacggaa acagtgcctg
      241 acgttcatcc gctggtggac gacatactgg ctgagactta cggcatcatg acgtaccagg
      301 agcaggtcat gatggtgctg aatcgtctgg gcaaactgcc gctgaatcgc gcgctgacgc
      361 tcatcaaggc tatcagcaag aaaaaggaaa aagtcatcgc cggcgaaaaa cccaatttca
      421 tcgtcggctg cggcgaaaac ggcattgacg aaaaacatgc cgaggaactt ttttcgctca
      481 tactgaaatt cgccggttac ggcttcaaca agtcgcattc gacccgctac gcgatagtgg
      541 cgtatcagac ggcgtatttc aaggttcact atccgcagga atttctggct gccacgctta
      601 cgtttgaatg cggcgacact gacaaggtcg tgcagtacat ggccgaggcc cgccgcatgg
      661 gcataaaagt tctgccgccc gacataaaca catgcggcgt ggattttgca gtcgacggcg
      721 acgcggtgcg tttcgggctg tccgcagtca agggcgtagg cccgacagcc gtcgaagaaa
      781 tcgtccgggc caggaaagaa gccggcggaa aattccacga cctttaccat ttctgtgaat
      841 tcgccgactt gcgcatagtc aaccgcggcg cgattgaatc gctgataaaa tgcggcgcgt
      901 tcgacgccat gggcgcgacc cgctcagcca tgaccgccgc cgccgacagc gcgatatcgt
      961 tcggcagcaa agccgccgcc gaccgcaaaa gcggccagat gagcttcttc gggtcgggcg
     1021 gccttctgag aggcaagagc gacgaacggc cgacgtttcc gaacgtcgaa ccgtggtcga
     1081 aaacgcaact cctcaaggcg gagaaggaaa cgctgggctt ttacgtatct gaccatccgc
     1141 tcgtggaata tggccgtgaa ctcagcggat tgagctatcc gccgcaaatc tcgctcggcg
     1201 caatcgagcc cgatgagcat aattacaggc acgacatgcg actgcgcatc ggctgcatga
     1261 ttcaggatgt gcggactgta ctgacaaaaa aggacagccg caagatggcc atgctcacgc
     1321 tggaggatac gtccggcaaa cttgacgcgg tcgtgtttcc gaatacgtac gagcagcttg
     1381 ccggcctgat tgtcaaggac gagctggttt ttattatcgg ccagcttgac cgcacgagag
     1441 aacggccgca gattatcgtc gatgaaatcc acccgatgaa ttcggcgctg caggatttta
     1501 cggcaggcat gactctgacg atgccccaca acgacaaagt cgccggcgaa tcgctactca
     1561 aaatactcca gaatcacaaa ggccactgcg aagtccgcgt aaaagtccgg ccgagctgcc
     1621 ggcccgacgt catggtgctc atctctctga gcagtaatct ctacgtccag cccagccgtc
     1681 cgctgatgaa agacctggaa aatttcctgg acgaccagaa tcttatagaa ctcgccccca
     1741 aacccgtccc gtcaggccgt caacgcaaag gttttcaacg caaatctccc caggaacaga
     1801 ccggacccgt cagcgaagcc gtcaccagat tcaactgaac cgtcaagacg gtatcatcac
     1861 agcagctgta tagagccata ttaaacttaa ctttgcgctc gttgctgtct caaatttaac
     1921 atgttctaaa atcattcgga tattttcaaa aatctgtcgc caggcaggac gcgatagccg
     1981 ttcacgaatg atttgaaatc catgtttttt cggccggctg gctgaatctc cagaattttc
     2041 agacggccgt cgccggtcgc tacgcagaat tcgtcgtcga ttgtgccagc cgcgccgcgc
     2101 gcctggcccg acgcaatttc gctgcgcgtt atgatgactc tagtggtttt gccgtcagcc
     2161 ttcgcaaaat cagccgccgc cccaggccat ggcgaacagc cgcgaatcct gtcatacacc
     2221 ctgccgacgg attgttcgaa atcgacataa ccgtccgact tctgcaattt cggcgcaaac
     2281 gtcgcaagtg aagcatcctg ttcgacaggc tgggcgatgc cggcttcgat gtcgtccagc
     2341 gtcgccagca caacttttgt gccgacggct gcgagttttt cccgcagttc gcccgcagtt
     2401 tcgttggggg taatctgcac tttttcggta cggtagaccg cgccggcgtc cattttgtca
     2461 accagttcaa acgtagtcac gccggactcc gcatcgccgt ttattacggc cctgttgaca
     2521 ggcgccgctc cgcggagagc cggcagaatc gacccgtgca gattgaaaca tgcgcggctg
     2581 acgcttttcc tggccgcctc gcgaataaat tggccatact ccgccacgac catgacgttg
     2641 gcgttgcacg attgcagaat ccgaagcgct tccgtcgaat tgatgtcctg cgtctcaagg
     2701 cagtcgattc cgcgccggta agccgcctgg gcgatgacgg tcggatttat tttaccgcct
     2761 ctgcccgatt tgcgaggagg ttgagtgacg gcgagcacaa ggtcgtgccg tgaatcggcg
     2821 acggcttcaa atgaaggcag tgcgaatgtt ccgcttccac agaatattat tctcataatc
     2881 cctcattgtg gccatcggct ggtcgatgtc aagacgaagg ggacaaaaag attcaatatc
     2941 ctttatgaca attccttgag aatcggctgt atgtcgttgc gcgcttttgc ataaacgacg
     3001 cactgataaa caagagcacc ggcaatcatc acggctgaag ccagataagg caatgacatt
     3061 gaaacactgt cggccagtac gccgccgaac agcggcccgg ctataccgca gccgcccacg
     3121 accatttcat ttatcgaaac atagcggccg gatttgctgg gatgcacgag cgaatgaaaa
     3181 acaaaataga aaaacatcga gccggaataa atcccgaaaa ttaccgccgc cgcgtaaaat
     3241 ccccatacga cgtggactac gccaaacatc gcggtcgcgg caacgccaag caagccatat
     3301 atgcagaccg gcaccggccg atacatccac gtacggctga aaatcatcga aaggccgctg
     3361 agtgcctgcg agccgctgag cagaaaaagc actatgccgg cttcaaagtc gctcaggttg
     3421 ttgactttcg cagtcttggc aaataacgcc cttataagta ttacgactga tattcccagg
     3481 cctgaactta tccagttcat caccgcgagg tcgggcattt ttgcatacga tgcatttttc
     3541 gggcgaatta caggctcgat ggttaacgaa tcgttctctg tccgtgcgtc ggcatcggcg
     3601 tgatgtttta acagataaat gcccacagat acgagaatgg acagcaggct tgtcatggcc
     3661 atgcaccatt gccaattatg atattgtaaa atgtaaccgg acaggaaagg cccaagcgct
     3721 atgcccattg accatgaaaa cgtatacaga gccgttgaac gaactacgcc gtcattatcg
     3781 ccaccgccga ttattttcat aaaatactgg aacggcgcaa aaaacaatgc aaccgatacg
     3841 ccagttaaaa ataccaatac gaacatggcc gttacgccgg aaaatattat gaaacagccg
     3901 ctcagtatcg ccatggacac gccggatgaa ataatcagcc aggctacatt gcggcgattc
     3961 gtccatatgc cgacgaaatg atttgccaaa agatacgaca ccgaccatat gctggctatg
     4021 gccgtcacaa gcgtagacga ccgaccagcc tgcgtcagtc gcataggatt tacaaacagc
     4081 agcaaaccta cggttatgtc cattactgcc ggaaacagga atattaaaaa tcgggtcatg
     4141 tttttttctc tcgttaattt ttattttatt ttatttttta aaatttttaa aaattttcaa
     4201 aaaatataca gccatgggtg ttttgagatg tattcaggaa tatcgctcca gaatggttga
     4261 gatgatatcg gcgatgttgg cgtttgcacc gcgcggatta gccgtgcata agcctgcggt
     4321 aagttcgagc atcgcgcggc gtgtctccag cagatatccg taatcgccca gcgcgtccag
     4381 aacgcaggca gtgatgctct ttacatcagg accgacgggc atgaattccg gcacaagctg
     4441 tttttgggca atgatgttga ccagcgaaag atattgcgta cggataagat gtcggatgaa
     4501 cagcttataa agtatccgct ccggcaggcc gatatgatat acgacggcca tgggcactcc
     4561 gaagtgcgcc acttccagcg taaccgtgcc gctggcggcg acggcgtagt gcgattccgc
     4621 caatacctca ggcgtacggg ctacagctat atccaggtcg tcgcgacggg aatatttttt
     4681 aatcatatcg gcttctcgcg ggccgacagc ggtgaactta caccgcgcga gcggatatgt
     4741 cctgcgaatg ccgtctgcta tccgcgacat catcttcgcg tgcagccgga tttcactcgg
     4801 ccgactgccc ggcagaaaag ctatcttcca ttcgccggaa gtccagggaa ttgcaaagtc
     4861 cggacattcg tcggcggaat gctgcggcgg aagctggtcg aacagcggat ggccgacgta
     4921 agtggctgca acgccgcggg aacgaaaata attttcctcg aacggcagca ggcaggctac
     4981 atggtcggtc aggcgtcgga ctttttttat ccgccacggc gcccacgccc agacctgcgg
     5041 agcgacgtaa tacatcaccg gcacgccgca gcgacgtgcc tgcttcgcaa gatgccagtt
     5101 caacgccggc gaatccaccg gcacaaaaat atcgggcttg acggcggtca tcgcacggca
     5161 gagctgccgg acttttttca tgtaatagaa cacgcctgtt accggaccgc ctagcataga
     5221 cgccttgtgc gtgaggtcgg cgatgacttc gcatccttca gcagccatgc atggcccggc
     5281 tgcgccgata aattccgcgg ctggatattt ttcgcgaaga cgacgaataa gctctgcggc
     5341 atggtgatcg cccgatgcct cagccgccga catgaatatg accggcgagg ctttttcatg
     5401 attttttttt gagctttttg agatgtccga aatattttta tcgtcggctg tcatttcgcc
     5461 gccctgccta cagccaccgc cgacccgact gccgccgaat caacgtgact gatggaaata
     5521 aatatttttt caatgccgag ctcacgagcg atttcatcgc acttgttata cagcttcacg
     5581 actggctggc cggacggaag attgcgaact tccacgtccg tccaggcgat gccgtcacgc
     5641 cagccggtgc ccaggacttt cagaacggct tccttgacgg caaaacggcc ggcataactg
     5701 accatcgcag acttttggcg ggaatcgcag tattgctgct cgaactccgt gaaaacgcgg
     5761 tcgagaaatt tttcgccaaa acgctcgatg ctctggctta accgcggcac gtcaaccagg
     5821 tctattccat gtgccaacac tgtttcactc atcgccggcc tcttcaagca actgacgaat
     5881 ttcaggttcg agaaatctat cccgtagcga ttcccggccg gctaatgcat ggacggcgac
     5941 gtttatctgg tcttccacgg aagcgtcggg atcaagaaaa agtatctccc tgcctttcag
     6001 ccgcaccagc gacccgccag gatgttcgtc gccaaacaat gacggcgcac gacgtacttc
     6061 catacccaac tcttcggcca gttcgaccag tttgtctatt tttgaaaatt cgtccataaa
     6121 taaataccga ctgtttcaaa atcaggctca aaaccgttca tactttcaga aacactttct
     6181 cagagaggga attttacttc atacgccgca atatgccaga tgaaaaataa ccgaaatctg
     6241 ttccaaaacg cttttttaca atttttacaa tttcaacact ctgtattcgg gaactttttg
     6301 atggttttta taatccaact acctaataga gggccagcga atgccggtat ccctttacag
     6361 agcgagttgt tgcaacatta ttttgccttg tgtcttatcg ttttgattat ttgcgtcata
     6421 aaaccaaaaa taacatatgc cgttgaaaaa taagacgtta tcgcgtcatt ttaccgtaac
     6481 tgaaacaaaa atcggctaaa atgaataata ttgttaaaaa attttgcccg gaaagcccga
     6541 aaatgttatc atatgacact ttgacggagg aagtctatcc tcacggaact gtacttcccc
     6601 actggagata gcttatgaat atgacagaag ttggaacggt aaaaacggga cttcttgcgg
     6661 ttttgttgtc agcagttttt gcgggcagtg cattttgtca gtccgagtac cctttcgagg
     6721 gagaagttac cggcacggac gtttatgttc gcagtgcgcc ggacatggcc agctatgccg
     6781 tagccaagat ttccaagcca gcccgcgtca ctgtgacggc taaagtttct gaaggctggt
     6841 atgcgataaa accaattcag ggctgttacg cggtggttga ccaggctttc gtcaaagccg
     6901 acaccagcga agcacggccg tcccgcgcca caggcgtaat cgtaggcgac aacgtccgca
     6961 tacgcgcggc gggcacgctc cggcgcagca atttcgacag catattgacg atggccgaca
     7021 aaggagaggc ggtaaccatc gtcggcacgg tcgaagaggg tgattcgacc tggtacataa
     7081 tattgc
//
LOCUS       DAONFI010000084         6928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_12751_length_6928_cov_4.285756, whole genome shotgun sequence.
ACCESSION   DAONFI010000084 DAONFI010000000
VERSION     DAONFI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_6928_cov_4.285756"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PLK08_03970"
     CDS             complement(<1..1266)
                     /locus_tag="PLK08_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52690.1"
                     /translation="MQIICGNCGQQIELDESAEAASIRCPSCSHEILLTDSHSNGIDE
                     NDGFASRARRLLSEAMQIECENCSLCLKVAKKMAGRRIRCPGCGYMINVPAEILDPEA
                     FEEVKPATAESVLAAVAQANSTTAEQPAASSHHHKPYKKPGMGVMPWILLLVAAGLVA
                     GIVYGIHVIKNKPAANLQNNPPDAENQLTLSQSVKPSDKMASVRILSCGWRMFANPAG
                     YFPAEQGRCYARLELQITTGDTPVEFLFDDTDAFIESGGTRYYAIGMELNAASIIPAD
                     VNRTPLKINPHTNLSGLRLIFNLPAAEFRGRLEIKGCNDQFVSLSAPPQYSVNLPGQY
                     IEHMPRNLKPMQHDPIIREFQLNYGLTLLASHSTGKVFAIKIPQAQISGTAELQPDRT
                     FVLRLSHPRHGMLTCKAVLSEDGKMLFVFL"
     gene            complement(1266..2930)
                     /locus_tag="PLK08_03975"
     CDS             complement(1266..2930)
                     /locus_tag="PLK08_03975"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPS52691.1"
                     /translation="MKIALAQINPTVGDLEGNYSKMAAMIDRAANLGAELVVFGELSV
                     MGYPPRDLLSRESFIRQSQETVERLAKHCMSTAAFVGYARPNPDKAGRPLQNVAAMLA
                     DGKIRHVHVKSLLPTYDVFDETRYFEPSQCPPPIEFKDKKLGVCICEDLWDEKSLGLK
                     LYHADPMENLKNAGAEIIINLSASPFENGKASVREELFSRQAHRCGCPIVYVNQVGAN
                     DELIFDGTSCVVDKHGEVIARAKSFEEDLLIVDMEGPGGKIEPRTDGIPRLTQALQLG
                     ISDYINKCGFSSVVFGLSGGIDSAVIAGLCANALGGEHVHCLAMPSRYSSEHSVSDAT
                     LMAQWLGLDYNIIPIENIHTAFEQAIDPLIAGTGSWGELAGENVQARIRGNLVMAWSN
                     AKGYLPLATGNKSELAVGYCTLYGDMCGGLAPIGDVLKTDVYAIAAHLNELRNGECIP
                     QNIITKAPSAELKPNQTDQDSLPPYDTLDKILRRYVELDMPVKEIIAEGFDDATVRKV
                     VRMVDRTEYKRQQAARVLKVSHRAFGMGRRVPIAQRFVDADAREKI"
     gene            3095..3769
                     /gene="rsmD"
                     /locus_tag="PLK08_03980"
     CDS             3095..3769
                     /gene="rsmD"
                     /locus_tag="PLK08_03980"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPS52692.1"
                     /translation="MRYFRDTDDYDEPDSRSNRNFRGTGGKILSLRIGSGFMAGRKLL
                     PPPTNDTRPMTGYAKKSLFSILRQRIENAVVLDLYCGTGTLGMEAISNGAAMVCFAEQ
                     NRRVVERLRQNIEALQVSDISRVWEGNLEMRLPDWLAKLNYKVDVAFVDPPYASARSW
                     DLDLIESRIFSPLAGSLADDGVILLRLPDDVPLPEKIGGLELNRTREYGSMKVGFYAR
                     KENVNV"
     gene            3803..4705
                     /locus_tag="PLK08_03985"
     CDS             3803..4705
                     /locus_tag="PLK08_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPS52693.1"
                     /translation="MAEKNTYKYFQPARAAKLGKLSLLARQCVEGFITGLHKSPNKGI
                     SAEFAEHREYAPGDDTRHLDWNVWGRSDRYYIKQYEQQTNLRACIALDCSESMAYGDG
                     GRLSKYEYGCFLAGVLAYLMTHQQDAVSLAKLGSDLQDLSPASSTPAHVDRMLRELER
                     TVPRGCGNLPVALHTLADRLQRRGMVVVISDFYSPLDEIVNALQHMLHKRHQVIIFHV
                     LDPSEIFLPFDGQVSVTDMETAARIEIDARAVRDEYNRQVATFLASLTRECHQRRIEY
                     VRAVTDVQYDRLLLKYLAARRGLK"
     gene            4702..6819
                     /locus_tag="PLK08_03990"
     CDS             4702..6819
                     /locus_tag="PLK08_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HPS52694.1"
                     /translation="MTFLSPWLLLGLAAVAVPVLLHMFWKSRAKAYAFATLMFLHSAV
                     VRTARRRKIQNWLLMLLRCGLIAMLTLAAAEPISRAGGFWGGKQRNAIVVLDDSFSMT
                     AKAGDMSAFENARAGVLELTARGDAPSQIAVLLTAGPTPSGFARLTSKIADIRQMVSS
                     AAISYAHASPDASLAGAAELAAQTPAEVFIFSDFQKNSFNLQKMLHNSSLDFFKKNSP
                     VYLIRSRKDSPSNLAITNLSVQGPNVRGASVNLMVTASNYSQADETVSVELVLPDTPP
                     LRKMLDLPADTSQVVAMPVELSQNGEIAGQVRMKTDDAIRCDDARFFAFTPRPVVRVA
                     VICDDENTDDPSAAGRVLSLALDWRTDAEKTTTPWPINVVQIKPSDLNATTLKTVDAV
                     FLCGVGGMSPAISKNLVDFARSGGVVMFFAGPAFDAGVYSSTLDGRLLPGGLAAPVGS
                     IGPDAPAWKVNSIDSADRLLGGLYQSADDFPQMNVQRFVPVKPSKDTKVLMSLENSFP
                     LLMRKDVGHGEVFWCTTAATPKWSNLPATGVFLPVVLRAALWQATGGDVPMCYVPGDF
                     VPLTGEGGWRFLPQISYTKKVTGKTAANGVVVDCRTAPGLVEYSAGNFNGVFALSPDP
                     AESDAECYDEDELLKKFHAADFANVYIGDDLGQVLAGASRERRGYNWRSLLACLAILT
                     LLVELAVSNRINVKQKAAVAEGS"
BASE COUNT         1478 a         1718 c         2025 g         1707 t
ORIGIN      
        1 gaggaacaca aacagcatct tgccgtcttc gctcagaaca gccttgcagg taagcattcc
       61 gtgtctcgga tggcttagcc gcagtacgaa tgtccggtca ggctgaagct ccgctgtgcc
      121 ggatatctgt gcctgtggaa tttttatggc aaaaaccttg ccggttgaat gtgaagcgag
      181 cagcgtcaac ccgtaattca gctgaaattc cctgataatt gggtcatgct gcatgggctt
      241 gaggttacgg ggcatatgtt cgatatactg gccgggcaga tttacggaat attgcggcgg
      301 agccgacaaa gatacgaact ggtcattgca gcctttgatt tccagccttc cgcgaaattc
      361 agccgccggc agattaaata tcagacgaag tccgctaagg ttggtatgag gattgatttt
      421 aagcggcgtt cggtttacgt cggctggtat tattgacgca gcgttcagtt ccataccgat
      481 tgcgtagtaa cgagttccgc cggattctat aaaggcgtcg gtatcgtcga aaagaaactc
      541 gacgggcgta tcgccggttg ttatctgaag ttccaggcgt gcatagcatc tgccctgttc
      601 agccggaaaa tagccggctg gatttgcgaa catccgccac ccgcatgaaa gtatccgcac
      661 cgatgccatc ttatcggatg gcttgacgga ttgggataaa gttaactggt tttccgcgtc
      721 gggcggattg ttttgcaaat ttgccgcagg tttgtttttt atcacatgaa tgccgtagac
      781 tatgcctgct accaagccgg ctgccaccag cagcagaatc cagggcataa cgcccatacc
      841 tggtttttta tacggcttat ggtgatgact tgatgcggct ggctgttcag ccgtagttga
      901 attggcttgg gcgacggcgg caagtacgga ttcggcggtg gcgggcttga cttcctcgaa
      961 cgcttccggg tccagaattt cagccggcac gtttatcatg tatccgcagc caggacatct
     1021 tatccggcgg ccggccatct tcttggcgac tttcagacag agcgaacagt tttcacattc
     1081 tatctgcatc gcctcgctga gcagccgcct ggcgcgagat gcgaaaccgt cgttctcatc
     1141 tatgccgttc gagtgtgaat cggtcaacag gatttcatgc gaacacgacg gacagcgaat
     1201 cgaggcggct tccgccgact catcgagttc aatctgctgg ccgcaatttc cgcatattat
     1261 ctgcatcaga ttttttctct cgcgtcggca tccacgaatc gttgggctat cggcacacgt
     1321 ctgcccatgc cgaacgccct gtgcgacacc ttcaacacgc gggctgcctg ctgacgctta
     1381 tattctgtgc ggtcaaccat tcggacgact tttctgactg tggcgtcgtc gaatccttcg
     1441 gcgatgattt ccttgaccgg catgtccagt tcgacgtatc ttcgcagaat tttatcaagc
     1501 gtatcatacg gcggcagcga atcctggtcg gtctggttgg gcttgagttc ggcgctgggg
     1561 gccttggtga tgatattctg cgggatgcat tcgccgttac ggagttcgtt cagatgcgcg
     1621 gctatggcgt agacgtcggt tttgagcacg tcgccgattg gagcaaggcc gccgcacatg
     1681 tcgccgtaga gcgtacagta tcccacggcg agttcggact tgttgccggt tgccagcggc
     1741 agatagcctt tggcgttcga ccaggccatt accagattgc cgcgaattcg ggcctggacg
     1801 ttttctcctg ccagctcgcc ccagcttcct gtgccggcaa tcagcgggtc tattgcctgc
     1861 tcgaaagcgg tatgaatgtt ttctattggg attatattgt aatcaaggcc cagccattga
     1921 gccatcaggg ttgcgtcgct gacggaatgt tcggaactgt agcggctcgg catggcaagg
     1981 cagtggacgt gttcgccgcc gagggcattg gcgcaaaggc cggcgattac ggctgaatct
     2041 atgccgcctg aaagaccgaa caccacgcta gaaaagccgc atttgtttat gtagtccgat
     2101 atgccgagct gcagcgcctg cgtcaatcgc ggaatgccgt ccgtgcgcgg ctcgattttg
     2161 ccgcccggcc cttccatgtc gacgatgagc aggtcttctt caaacgattt ggcgcgggct
     2221 atcacctcgc cgtgtttgtc cactacgcaa cttgtgccgt cgaagatgag ttcgtcattt
     2281 gcaccgacct ggttgacgta aactatcgga cagccgcagc ggtgggcctg ccgcgaaaaa
     2341 agttcctcgc gaacggacgc cttgccgttt tcaaacgggc tggcggaaag gttgatgatt
     2401 atttcagcgc cggcgttttt gagattttcc atcgggtcgg cgtgataaag cttcaggccc
     2461 agagattttt catcccagag gtcttcgcat atgcacacgc cgagcttttt atccttgaat
     2521 tcgattggcg gcggacattg cgacggctca aaatagcggg tctcgtcgaa aacgtcgtag
     2581 gtcggcagaa gcgattttac gtggacatgc cttattttgc cgtcagccag catggcggcg
     2641 acgttctgaa gcggccggcc ggctttgtcg ggattcggcc gtgcatagcc gacgaacgca
     2701 gccgttgaca tgcaatgctt agccagccgc tctaccgtct cctgtgactg gcggatgaac
     2761 gactcccgtg agagaaggtc gcggggcgga tagcccatta cggacaattc cccgaatact
     2821 accagttccg cgccaagatt tgcggcgcgg tctatcatgg cggccatttt cgagtagttg
     2881 ccctcaaggt cgccgaccgt cggattaatt tgtgcaagcg ctatcttcat cgtcatgatt
     2941 ttaaatattt gcgggcgaaa tgagaaatgg gaaattgtaa aacgggaatt aaaattgctc
     3001 aaatatgtca gacggctgag caagagaaac ttttgggatg gatttgcggt tcattcttgc
     3061 atagtttcag ctgtgacgat attttattgg tcatatgaga tatttcagag atactgacga
     3121 ttatgacgag cctgacagcc gcagcaatcg gaattttcgt ggcacgggcg gcaaaattct
     3181 ctcgcttcgc atcggcagcg gattcatggc cggcaggaaa ctgctgccgc ccccgacgaa
     3241 cgatacacgg ccgatgaccg gttatgcgaa aaaatcgctt ttcagcatat tgagacagag
     3301 aatagaaaac gccgtcgttc tcgacctgta ctgcgggacg gggacgcttg gcatggaagc
     3361 gataagtaac ggagcggcga tggtgtgttt cgccgagcag aaccgtcggg ttgtagagcg
     3421 gctaaggcag aatatcgagg cgttgcaagt gtcggatatc agccgcgtat gggaaggcaa
     3481 tcttgaaatg cgactgccgg actggctggc gaaactcaac tacaaggtgg acgtggcgtt
     3541 tgtagacccg ccgtatgcct ccgcccgttc atgggacctg gaccttattg aaagccgcat
     3601 attttcaccg cttgccggca gccttgccga cgatggtgtg atacttctgc gattgcccga
     3661 tgatgttccg ctgcccgaaa aaatcggcgg acttgaactg aatcgcacca gggaatacgg
     3721 cagcatgaaa gtcggattct atgcccggaa agaaaacgtg aacgtataaa tataaatgta
     3781 aaaaaacttt cagctggatt ttatggccga aaagaatacg tacaaatatt ttcagccggc
     3841 tcgcgctgcg aaactgggta aactcagcct gcttgccaga cagtgcgtgg aagggttcat
     3901 aaccggtctg cataaaagtc cgaacaaggg gatttcggcg gaattcgccg agcatcgcga
     3961 gtatgcccct ggcgacgata cgcggcatct ggactggaac gtatggggcc gcagcgacag
     4021 gtattatata aagcaatacg aacagcagac gaatttacgg gcgtgcatag cgttggattg
     4081 cagtgagtcg atggcctatg gcgacggcgg cagactgagc aagtatgaat acggctgttt
     4141 tctcgccggc gtgcttgcgt atctgatgac gcatcagcag gatgcggtgt cactggcgaa
     4201 gttgggtagt gatttgcagg atttatcgcc agcctcgagt acgcctgctc acgttgaccg
     4261 catgttgcgg gagcttgagc ggactgtgcc gcgcggttgc ggcaatctgc cggtcgcact
     4321 gcataccctg gctgaccggc tgcagcgacg cggcatggtg gtggtgattt cggattttta
     4381 cagcccgctc gatgagattg tgaacgcctt gcaacacatg ctgcataagc ggcatcaggt
     4441 catcattttt cacgtgcttg acccgtcgga gatttttctg ccgttcgacg gccaggtctc
     4501 ggttacggat atggaaaccg ccgcgcgaat cgaaattgac gcgagggctg ttcgcgacga
     4561 atataaccgg caggtcgcga cgtttctggc cagcctgaca cgtgaatgtc accaacgcag
     4621 gattgagtac gttcgtgcgg ttacggatgt gcagtatgac aggcttctgc tcaaatatct
     4681 cgcagccaga aggggattga aatgacgttt ctttctccgt ggctgctttt ggggctggcg
     4741 gctgttgcag tgccggtatt gctgcacatg ttctggaaga gcagggcgaa ggcctatgct
     4801 tttgcgacgc tgatgtttct gcactcagcc gtagtgcgta cggcgcggcg gcgaaagatt
     4861 cagaactggc tgctgatgct tttgcgatgc ggacttattg cgatgctgac attggctgcg
     4921 gctgaaccta tatcgcgtgc cggcggtttt tggggcggaa agcagcggaa cgcgattgtg
     4981 gtacttgacg acagtttttc catgacggcc aaggctggcg atatgtccgc gtttgaaaat
     5041 gcccgcgcag gcgttttgga actgactgct cgcggcgatg cgccgtcgca gattgccgtt
     5101 ttgctcacag ccggcccgac gccgtctggt tttgcacgcc tgacttcaaa aatagccgat
     5161 atacgccaga tggtttcttc cgctgcgata tcgtatgctc atgcttcgcc ggatgcctcg
     5221 cttgccggag ctgccgaact tgccgcgcaa acgccggcgg aagttttcat tttcagtgat
     5281 tttcaaaaaa acagtttcaa tttgcagaaa atgctgcaca attccagcct ggattttttt
     5341 aaaaagaatt cgccggtcta tctgattcgg tcgaggaagg attcgccctc aaatcttgcg
     5401 attaccaact tgagcgtgca aggccccaac gttcgcggcg cgagcgtgaa tttgatggtt
     5461 acagcgtcga actattcgca ggcggatgag actgtgagcg tggaacttgt tctgcccgat
     5521 acgccgccgt tgagaaaaat gcttgacctg ccagccgaca cgtctcaggt agttgcgatg
     5581 cctgtggaat tgtcgcaaaa cggcgaaatt gccggccagg tacgcatgaa aacggatgat
     5641 gccattcgtt gcgatgacgc cagatttttt gcgtttactc ccaggcctgt cgttcgggta
     5701 gccgtgattt gtgacgatga aaataccgac gacccatctg ctgccggccg cgtattgtcg
     5761 ttggcgttgg actggcggac ggatgctgaa aaaacaacaa cgccctggcc gattaatgtt
     5821 gtgcagatta agccgtcgga tttaaatgcg acaaccctga aaaccgtcga tgcggtattt
     5881 ctgtgcggtg tgggagggat gtcgccggct atatcgaaaa atcttgtcga ttttgcgcgt
     5941 agcggcggag tggtgatgtt tttcgccggc cccgccttcg atgccggcgt ttacagttct
     6001 actcttgacg gaaggctgtt gccaggcggg ttggctgcgc cggtcggttc gatagggcct
     6061 gatgcgccgg cgtggaaagt caatagcatc gactctgccg acaggctgct cggcgggctg
     6121 tatcagtccg ctgacgattt tccgcagatg aacgtacagc gttttgtgcc tgtaaaaccg
     6181 tctaaagaca caaaagtatt gatgtcgctt gaaaattcat ttccgctatt gatgcgaaag
     6241 gatgtcggcc acggcgaggt tttctggtgt acgacagccg ccacgccgaa atggtccaat
     6301 ctgcctgcga ccggagtttt tctgcctgtg gttttgaggg ctgcactttg gcaggctacg
     6361 ggtggcgatg tgccgatgtg ctatgtgcct ggagattttg tgccgttaac aggcgaaggc
     6421 ggctggcggt ttctgcccca gatttcttat acgaaaaaag tgacaggaaa gactgcggcg
     6481 aatggcgttg ttgttgattg tcgtactgct ccggggctgg tggaatattc agcgggtaat
     6541 tttaacggcg tattcgcgtt gagccccgac cctgccgaaa gtgatgccga atgctacgac
     6601 gaggatgaac tgctcaaaaa attccatgcc gcggattttg cgaatgttta cattggcgac
     6661 gatttgggcc aggtgcttgc aggagcgtcg cgtgaacggc ggggatataa ctggcggagt
     6721 ctgctggcgt gtctggcaat attgacgttg ttggttgaac ttgcggtttc caacagaata
     6781 aatgtgaaac aaaaggctgc agtggcagag ggcagttaac ttttctgaca ctgaaccggc
     6841 ggatggccgt aactgcaaag caacaagatg cgagattcgc ttcgcggcgc cttcgctacg
     6901 ctgcggcgtc aaacccgcga agtgtctt
//
LOCUS       DAONFI010000085         6900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_12808_length_6900_cov_3.486048, whole genome shotgun sequence.
ACCESSION   DAONFI010000085 DAONFI010000000
VERSION     DAONFI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12808_length_6900_cov_3.486048"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1143
                     /locus_tag="PLK08_03995"
     CDS             208..1143
                     /locus_tag="PLK08_03995"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPS52695.1"
                     /translation="MAIKKSMLQRIVFGSIMIVLLAGVFVGDYLLGMYREYYSGVLLS
                     LIFLPVLIIGYLEYSKMAGSVGVRTLNISGIAGVFVLSFMPVICNFAGGFGLDSCRHA
                     GSLQNLLIWIARLTSFLVPWLGVAFLPIFLEQMWRYRLADAIRQVACTIFGVLYIGML
                     GSMLMQLRMTGSVLFLVFVVAMVKFTDIGAYFTGSFVGRHKMIPWLSPGKTWEGLAGG
                     LIFASVIGVLGYYMARGCGIELFVNSPVTAAIMGLTLGIVGQFGDLCESLLKRAAGVK
                     DSGACIPQFGGVLDLLDSPLIAAPIAYLWLILLKY"
     gene            1232..2455
                     /locus_tag="PLK08_04000"
     CDS             1232..2455
                     /locus_tag="PLK08_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPS52696.1"
                     /translation="MIHIVRGLFLLSVLVITASFAMKVVSNAERKTQTDASMQQVNNT
                     DETSPFLTGKAVKPLESPRVQPTSDVVTYSSDNMVNFITLYILTPALCACGIVFVDMF
                     WRKKQLSVLGGLFFGVLAGLALAYILGMIVDLVLEVFQVPDTDPIVALVKYLLGATVI
                     FFCITIVLQTKDEFRFIIPYVEFSKNTKGNQPVLLDTSVIIDGRIADVVETKVLQSEL
                     IVPRFVLTELQAVADSEDKLKRNRGRRGLDVLQKLQMSDKVDVNIVEFHLPTVQGADD
                     VDAKLVALGEHLGARIITNDYNLNKVARLKGLDVINLNDLANALKPIVLPGERMKVKI
                     IKPGEEPGQGVGYLDDGTMVVVDYARSQMGKMVNITVTSVLQTSAGRMIFGKYEDELA
                     SVNATDENETNINNG"
     gene            2505..3197
                     /locus_tag="PLK08_04005"
     CDS             2505..3197
                     /locus_tag="PLK08_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HPS52697.1"
                     /translation="MSEKTLSTQMSELRISTGENLENAGPFIAISREYGCGGFSLGLL
                     LLDLLNDEVDDGRGWQIYHKEILQTLASETNLAMEIIERQRRDRPNLLVNFFKTLAGQ
                     KNHIPSGMEIRKRMTHIIRELAVEGRAILIGQGSTGAVADLPRGLSVRLEAPEDWRVK
                     QVAFREGLNEIDARRLIREETERREYLRKIYERKFILKPSFSMTFDCSKFSLSQIAML
                     IYRAMKLKDWVR"
     gene            3427..4059
                     /locus_tag="PLK08_04010"
     CDS             3427..4059
                     /locus_tag="PLK08_04010"
                     /inference="COORDINATES: protein motif:HMM:NF017952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrpE/YscL family type III secretion apparatus
                     protein"
                     /protein_id="HPS52698.1"
                     /translation="MSETIEDFVAKLQMEGVQAGQTKADEIIASAKSQAADIVAEAKK
                     QAQKILSDAKTDAQSTVARSKTELNLAARDTVGRLRETLCDCINLIIAQAATQKLDDI
                     DFIGDILKEIVDQYVKADIKGKTLIDINVSPQNREQLKAWAFKTLGQTVVEKFRPCIQ
                     IKDKLNQAGFEFSVETGTIEVTRDSVVDILSDMVTPELRKILAGSSGNPE"
     gene            4072..4788
                     /locus_tag="PLK08_04015"
     CDS             4072..4788
                     /locus_tag="PLK08_04015"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPS52699.1"
                     /translation="MAGKNYYIQSFLAELDTIESPVKISTGDFLDMLEPDTPVTQAVR
                     LIFLSDDLLQRESCLAGQIRQVSPAVLSAEILRGEEPLPDFLLSDVPTARKIESDAVW
                     ENYFRYAAKNVSPTAAGNFLRQWIAFEVTLRNSLVEARAGSLGVEPADFFVADELSDS
                     SIDVKQAVAEWLNTVTDNPIAAQKILDRARWDWISQKGGYFSFDDDELVAYAAKLSIA
                     CRWKCLAEEMEKSQSEKSSN"
     gene            4834..6570
                     /locus_tag="PLK08_04020"
     CDS             4834..6570
                     /locus_tag="PLK08_04020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPS52700.1"
                     /translation="MTGKIVAVFGNMIVAEVDGDVVQNSVGYCCRDDGARLLSEVIRV
                     RGRCVDLQVFEETRGLKVGNRVEFEDKMLSLVLGPGLLGQVYDGLQNPLPRIAEATKD
                     EKGGFFLKPGYYIHGLDLAKKWSFTPTSKTGDVVQAADSLGSVPEGLFTHQIMVPFTL
                     SGEAKVKNIVPAGDYTVDTQIATLEQNGAEIPVTMQQYWPVKKALSICKRRLMPTEPL
                     ITKVRIIDSMFPIVKGGTYCIPGPFGAGKTVLQHITSRHAEVDIVIVAACGERAGEVV
                     ETIREFPALVDPRTGRSIMERTIIICNTSAMPVAAREASVYTGVTLAEYYRQMGLNVL
                     LLADSTSRWAQAMRELSGRLEEIPGDEAFPAYLESRIAGFYERAGLIELHDGRLASVT
                     IGGTVSPAGGNFEEPVTQGTLKVVGAFHGLSYARSYARRYPAIDPLDSWSKYTGIIEP
                     ERVNRMRSILRRGNEVKQMMTVVGEEGTPIDDFTIMLKAEMFDNCYLQQNAFDETDGA
                     TPADRQRFVFDLILRACDTDFVFESREDARQTIFTLQDMLRNWNYAPYESEEYRDFVS
                     KIENFIDKKGKI"
     gene            6583..>6900
                     /locus_tag="PLK08_04025"
     CDS             6583..>6900
                     /locus_tag="PLK08_04025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPS52701.1"
                     /translation="MACRVNIGCGNAPTVGESWKNYDNSLSIVLAKWPTEFIRLLRKI
                     RVLSSANVDAIAGYKKFGSLIRRADCTKLPLTNDSVDVVYTSHMFEHLSKAKAERFLR
                     EAYR"
BASE COUNT         1694 a         1568 c         1927 g         1711 t
ORIGIN      
        1 ccctgggaac ggcggtctgg ggcggcatga tagcggcgac ggtgataggc ctgttctttg
       61 tgccggcgct atatgtcatc gtccagactt atgccgactg gctggcgaag cggcggcaga
      121 tgaaaacacc gccggcggca gataatgaat ctgccggctg atgtgaaata tccggttttt
      181 tgaaaaaaag aatcatatga ggtaatcgtg gctataaaaa aatccatgtt gcagaggatt
      241 gttttcggtt cgataatgat agtgctgctt gccggcgtat tcgtaggcga ctatctgctc
      301 ggcatgtatc gggagtatta cagcggtgtg ctgttgagcc tgatttttct gccggtattg
      361 ataattggct acctggaata ctcgaagatg gctggcagtg tcggcgtaag aacgctgaac
      421 atttccggca ttgccggcgt atttgtgctg tcgtttatgc cggtgatttg caattttgcc
      481 ggcggatttg gtctggattc gtgccggcac gccggcagtt tgcagaatct gcttatatgg
      541 attgcgcggc tgacgtcgtt tcttgtgccg tggctgggcg tggcgttcct gccgatattt
      601 ctggaacaga tgtggcgata tcgtctggcg gatgcaatac ggcaggttgc ttgtacaatt
      661 tttggagtgc tctatatcgg catgcttggc agtatgctca tgcaactgcg gatgacaggg
      721 tcggtgcttt tcctggtttt cgtggtggcg atggtgaaat ttaccgatat cggggcatat
      781 tttaccggct cgtttgttgg ccgacacaag atgataccgt ggctgtcgcc tggcaagaca
      841 tgggaaggtt tggccggcgg attaattttc gcctcggtaa ttggcgtttt gggatattac
      901 atggctcgcg gctgcgggat tgaattattt gtcaattctc cggtaactgc tgcgattatg
      961 gggctgacgc tgggaatcgt cggccaattc ggcgatttat gtgaatcgct gcttaaacgg
     1021 gcggctggag tgaaagattc cggcgcatgt ataccgcagt tcggcggcgt actcgattta
     1081 ctggattcgc cgcttattgc cgcgccaatt gcatatctct ggttgatttt gttgaaatat
     1141 tgacaataat gtggaaatat atattcgccc atcgtagggg cgacggttta atatctttta
     1201 tattttagtt cagttttata aggagacgaa catgattcac attgtcagag gtcttttcct
     1261 tttgtcggta ttggtgataa cggcgtcatt cgcgatgaaa gtcgtttcca acgccgaaag
     1321 aaaaacgcag actgacgcaa gcatgcagca ggtgaataat accgatgaga cgtcgccttt
     1381 tctgaccgga aaagccgtca agccgctgga gtctccacgc gttcagccga ccagcgatgt
     1441 ggtaacctac agcagcgata acatggtcaa tttcataacg ttgtacatcc ttacgcctgc
     1501 gttatgtgcg tgcggcatcg tgtttgttga tatgttctgg cgtaaaaagc agctgagcgt
     1561 tctgggcggt ctgtttttcg gcgtgttagc cggcttggcg ttggcgtata tactgggcat
     1621 gattgtcgac cttgtgcttg aagtcttcca ggtgccggat accgacccca tcgtggcgct
     1681 ggtcaaatac ctgctcggcg cgacggtgat atttttctgc atcaccattg tgctgcaaac
     1741 caaagatgaa ttccgcttta tcattcctta cgtggaattt tccaaaaata ccaagggcaa
     1801 tcagccggtt cttctggaca cgtctgtcat tatcgacggc cgcatcgccg atgttgttga
     1861 aaccaaggtg ctccagagcg aacttatcgt gccgcggttc gttctgacgg aactgcaggc
     1921 ggtcgccgat tccgaggata aactcaaacg caaccgtggc cgtcgcggct tggatgtgct
     1981 ccagaaactc cagatgagcg ataaggttga cgtaaatatc gtggaatttc atctgccgac
     2041 ggtgcaggga gccgacgacg tcgatgcaaa actcgtagcc ctcggcgaac atctcggcgc
     2101 acggattatc accaatgatt acaatttgaa caaggttgcg cggcttaaag gtctggacgt
     2161 aatcaatctc aatgaccttg ccaacgctct caagcccata gttctgcccg gcgaacgcat
     2221 gaaagtaaaa ataatcaaac caggcgaaga accaggtcag ggcgtcggct atctcgacga
     2281 cggtacgatg gttgtcgtgg attacgcccg cagccagatg ggcaaaatgg tcaatataac
     2341 tgttaccagc gtgctgcaga catcagccgg ccgaatgatt ttcggcaaat acgaagatga
     2401 attggcatct gtaaacgcta cggatgaaaa cgaaaccaat ataaataatg gataaaaaaa
     2461 tatagaattt tcttgtctct taattttttt ggagataata tctgatgtcc gaaaagaccc
     2521 tctcaaccca gatgagtgaa ctgcggattt caaccggcga aaatctggag aatgctgggc
     2581 ctttcattgc catatcccgc gaatacggct gcggcggttt ttcgctgggc cttttgctgt
     2641 tggatttgct gaacgatgaa gtcgacgacg gcagaggatg gcagatatat cacaaggaaa
     2701 ttcttcagac gctcgcgtcc gagacgaacc ttgcgatgga aattatcgag cggcagcggc
     2761 gtgaccggcc caacttgctg gtgaattttt tcaaaacgct ggctggccag aaaaaccaca
     2821 tcccaagcgg gatggaaata cgtaaacgca tgacgcacat tatccgcgag cttgcagttg
     2881 aaggcagggc gatactgata ggtcagggtt caaccggtgc ggtggctgat ttgccgcgag
     2941 gtttgagcgt acggctggaa gcgccggaag actggcgggt aaagcaggtg gcgtttcgtg
     3001 aagggttgaa tgaaattgac gccaggcggc tgattcgcga agaaaccgaa cgacgggaat
     3061 atcttcggaa aatttacgaa cggaagttca ttctgaaacc gtcgttttca atgacgttcg
     3121 attgcagcaa gttcagcctc tcgcagatag cgatgctgat ttaccgcgcg atgaaactta
     3181 aagattgggt caggtaggct tttcggcggc gacatagaaa ttttaagaaa aattttgaag
     3241 ccgggtgttt ttacgcatgc cacaaaagat gaaaaatcta taatgacgta ttgtcgtctg
     3301 ttttgaacga aaaatgtaaa agatgttgaa aagtttaggg catttaaaaa atttaaaaat
     3361 ttgaaaaatt gtaacggttt ttcaaaaaac cctgccgagc agtttttggt gtcaggagtt
     3421 gaggaaatgt ctgagacaat tgaagatttt gttgctaagt tacagatgga aggcgtacaa
     3481 gccggccaga caaaggctga tgagataatc gcctcagcca aatcgcaggc tgccgatatc
     3541 gttgccgagg cgaaaaaaca ggctcaaaaa attctttcag acgccaaaac tgacgctcaa
     3601 agtacggtcg cacgctccaa gactgaactg aatctcgcgg cacgcgatac tgtcggccgt
     3661 ctgcgggaga cgctctgcga ttgcatcaac ctgattatcg cgcaagccgc gacgcagaaa
     3721 cttgacgata tagattttat cggcgatata ctcaaggaaa tagtcgacca atacgtcaaa
     3781 gccgacataa aaggcaaaac ccttatcgat atcaacgtta gtccgcaaaa tcgcgaacaa
     3841 ttgaaggcat gggcattcaa gaccctcggc cagacggttg tggagaaatt ccgcccctgc
     3901 attcagataa aggataaact caaccaggcc ggttttgaat tttcggtgga gacgggtact
     3961 attgaagtta cccgcgattc cgtggttgat atactctcgg acatggtcac gccggaattg
     4021 cgaaaaattc tggctggctc atcgggaaac cctgaataat cggatgtcaa agtggctgga
     4081 aagaattatt acattcagtc gtttctggca gagcttgata caattgagtc ccccgtgaaa
     4141 atttcgacgg gagattttct ggacatgctc gagccggata ctccggtgac gcaggctgtc
     4201 aggctgattt tcctgtctga cgacctgttg cagcgagagt catgtcttgc cggccagatt
     4261 cggcaggttt cgccggcggt gctttcggct gagattctga gaggcgaaga gcccttgccg
     4321 gatttccttc tgtcggatgt ccccaccgcc aggaaaatcg aatccgacgc cgtgtgggaa
     4381 aattatttcc gatatgccgc aaaaaacgtt tcgcccacag ccgccggtaa ttttttgaga
     4441 caatggattg cgtttgaagt cacactgaga aattcgcttg ttgaagctcg tgcaggttca
     4501 cttggcgttg agcctgcgga ttttttcgta gccgatgaac tttcagattc ttcgatagac
     4561 gtcaaacagg ctgttgccga atggctgaat acagtaaccg acaatcccat tgcagctcag
     4621 aaaattctcg accgtgcccg atgggactgg atatctcaga agggcggata tttcagcttc
     4681 gatgacgacg agctggttgc ctatgccgca aagctttcca ttgcctgtcg ctggaaatgt
     4741 ctcgccgaag aaatggaaaa gagtcaatcg gaaaaaagtt caaactgacc ggcgttttcc
     4801 gtattcgccg gctgcttgtg aaaggataaa accgtgactg gaaagatagt tgcagttttc
     4861 ggaaacatga tagtcgccga agtcgatggc gatgtcgtcc agaattcagt gggctactgt
     4921 tgtcgtgatg acggtgcgcg gctgttgagt gaagttatcc gcgttcgcgg cagatgtgtt
     4981 gacctgcagg tgttcgagga gacgcgcggc ttgaaggtcg gcaatcgtgt agagtttgag
     5041 gacaagatgc tttcgctcgt gcttgggcct ggtttgctgg ggcaggtata cgacggcttg
     5101 cagaatccat tgccgcgtat tgcggaggct accaaagacg agaagggcgg attcttcctc
     5161 aaacctggtt attacattca tggccttgat ttggcaaaaa aatggtcgtt tacgcctacg
     5221 tccaaaactg gcgacgtggt acaggcggct gattcgctcg gcagtgtgcc ggaaggcctc
     5281 tttacccatc agataatggt gccgtttacg ctctccggcg aagcgaaagt caagaatatc
     5341 gtgccggctg gcgattatac cgtcgatacg cagattgcga cgctcgaaca gaacggcgcg
     5401 gaaattcccg ttaccatgca gcaatattgg ccggtcaaga aggcgttgag catctgcaag
     5461 cgacgtctca tgccgaccga gccgctgatt accaaagtac gcataatcga ctccatgttc
     5521 ccgatagtca agggcggtac gtattgcatt ccggggccct tcggcgcggg caagaccgtg
     5581 cttcagcata ttacgtcgcg acacgcggaa gtggacatag tgattgtggc ggcttgcggc
     5641 gaacgtgccg gcgaagtcgt cgaaaccata cgcgagtttc cggcgctcgt cgacccgcgt
     5701 accggccgaa gcatcatgga acgtacgata atcatttgca atacctcggc gatgcccgtg
     5761 gcggcacgcg aggcgtcggt atatacgggc gtgacgctgg cggagtatta tcggcagatg
     5821 ggtttgaatg tgctgctgct ggcagactcg acgtcgagat gggcgcaggc gatgcgtgaa
     5881 ctttcaggca ggctggaaga gattcctggc gatgaggcat ttccggcgta tctcgaaagc
     5941 cgcatagccg gcttttatga gcgtgccggc ctgattgaac tgcacgacgg ccgactggct
     6001 tccgttacaa tcggcggcac ggtcagcccg gcgggcggca acttcgaaga gccggtgacg
     6061 cagggtacgc ttaaggtcgt cggagcattt cacggcttga gctatgcacg ttcttatgcc
     6121 cgccgctatc cggctatcga cccgctggat tcgtggagca aatatacggg cataatcgaa
     6181 cctgaaagag tcaacaggat gcgcagcatt ctgcgacgcg gcaatgaagt caagcagatg
     6241 atgaccgtcg tcggagaaga aggtacgccg atagatgatt ttaccataat gctcaaggct
     6301 gaaatgttcg acaactgcta tctgcagcag aacgcgtttg atgagaccga cggcgctacg
     6361 ccggctgacc ggcagcggtt cgtgttcgac ctcatccttc gcgcatgcga tacggacttc
     6421 gtgttcgagt ccagggaaga tgcgcggcag actattttta cactgcagga tatgctcaga
     6481 aattggaact acgccccgta cgaaagcgaa gaatacagag atttcgtaag taaaattgaa
     6541 aatttcatag ataaaaaagg caaaatctga gactaaaata ttatggcttg ccgagtcaac
     6601 ataggatgcg gaaatgcccc gaccgtaggc gaaagctgga aaaactatga caatagcctt
     6661 tccatcgttc tcgccaagtg gccgactgaa tttatcaggc tgctgcgcaa aatccgcgtt
     6721 ttgtcttccg caaatgttga cgctatagcc ggctataaga agttcggcag tttaattcgt
     6781 cgtgcggatt gtacgaaact gccgctgacg aatgatagtg tcgacgttgt ttatacatcg
     6841 cacatgtttg agcatctttc gaaggccaag gccgagaggt ttcttcgcga ggcttacagg
//
LOCUS       DAONFI010000086         6794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13066_length_6794_cov_3.342336, whole genome shotgun sequence.
ACCESSION   DAONFI010000086 DAONFI010000000
VERSION     DAONFI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13066_length_6794_cov_3.342336"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="PLK08_04030"
     CDS             <1..1733
                     /locus_tag="PLK08_04030"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPS52702.1"
                     /translation="LTGQMNGLISKFADEYGTLRFDVPEGSYRLVLMSSRVLYDGTHV
                     TENLSDPRYCVNLADRDATRKFIEVTHEKYFEVCGEEFGKSILAFFTDEPSLIAWHIR
                     PGTFPMLPWHSSFPQEFKKRYGYPIVDAVLAVFRNAGEQTVKRRCDFWEFIADQVAAN
                     YFGEIQEWCHAHGLKSSGHMLWEEKLCWHVHNYGSFYRCAKRLDWPGIDQLSCVPQQL
                     MEADYIPISRLLASIADISGEKESFTEFSDFAATPQNVHISIDWIRATVNWHLAQGIN
                     NFTSYHLWNRYKDTEIRELNDYTARLSQIMHQGKRDSRVAVLYPEATIWNAFNPSAGG
                     DDAKEKNGHISRIDNVFAVTSWELLKSHIDFDYVDDQLLLDARLVNGKLLYKNRTYEC
                     LILPAVEVLGKAIIDKIVAMLRAGVKVIFVGGEPELIRDNGDKIDFLSLLSEFVSNQD
                     FERISATNENMFKTSKLLPRTFRLQMKDYTAAVGAYNIPADNLIRIQMTHSRILDDGT
                     RLVMVANMGGSSFIGNIAIDTPCGDVRIADPHTGKIIAAKTTRQPNQTEIAVTLPAYD
                     SLVFIIDPAR"
     gene            1985..2692
                     /locus_tag="PLK08_04035"
     CDS             1985..2692
                     /locus_tag="PLK08_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPS52703.1"
                     /translation="MQLSVKDTARLLNVSEKTIYRWIKQQVIPVYKINEQFRFNRSEL
                     LEWATSRRIQVSPEIFKETEQNGTLLPSLADAFKAGGIFYRVGGDDTPSVLRAIVDVL
                     NLPEEVDREFLYQVLMARETLGSTGIGDGIAIPHVRNPVVLHVSRPSISLCFLDNPID
                     FLAIDGQPVNTFFTLISPTVRAHLHMLSRLGFVLQNKEFRSAIKKQAPREEIISSLAA
                     AEATLPDAANSNGDQLK"
     gene            2776..4776
                     /locus_tag="PLK08_04040"
     CDS             2776..4776
                     /locus_tag="PLK08_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPS52704.1"
                     /translation="MSLTLITIACIISAASGLPGLFSSRPSSWGQKLAAAMMCTSAIV
                     GLAGAFDGFFATSVSERFFPWPAAGNSFIGIDALSAFFLCPVFLMGGLGAIYGLGYWP
                     QRLHMRNARQLQLFWGLTTAGMVLLVISRHAMAFLLGWEIMALSAFFLIAAEDDREEC
                     RRAGWIYLIATHVGSLVLFGMFMLWRWATGSYMFTPTAAGGALSLCVMNVLFFMSLVG
                     FGVKAGIMPLQFWLPGAHANSPSHVSAMMSGVVLKMGVYGLVRFLWLFPNPPHMWGGL
                     ILILGATGGLLGVVFAIAQHDIKRLLAYHSVENIGIILMGFGLAMLGRSSGRQEWVVL
                     GMAGCLLHVWNHSLFKSLLFFSAGSVVHASHTREIDRLGGLAKKMPWTAAMFMIGAVA
                     ICGLPPLNGFVSEFFVYVGLLKSFVGDGATGAAAVMVVPVLAMIGALAVACFVKVYGV
                     VFLGNPRTPAAEHVHESPQTMRWPMAVLAACCFIIGLAPITVVPILNRAIAVWNFGAA
                     QSVATAVPLTTVSTLAMVFAVVIVAMLLIAAVKKWPARRSVTWDCGYAQPTARIQYTA
                     SSFGRTIVLLFNWVLRPREHRPDIQGLFPKPSKIHTHVDDIVLDGAILPASRRISRWF
                     MWFRRFQQGIVQNYVFYILLAVIVMLCTLMPFGKIFTRLFSR"
     gene            4800..5708
                     /locus_tag="PLK08_04045"
     CDS             4800..5708
                     /locus_tag="PLK08_04045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPS52705.1"
                     /translation="MIEKIIHIAILLVLPPLLLGVINRIKAKFAGRVGPPLFQTYYDI
                     IKLMRKDMVISDTTSWVFIAGPAITLVAVLLAGLLIPLGRFAAPINFTGDLILFAYLF
                     ALARFFTTAAALDTGSSFEGMGAAREVTFACLSEPATFFAMLVLAKVSDSLTLTNMLH
                     GPAGGFSIAVAAPLVLIAIGLFIVLLAETCRIPVDDPNTHLELTMIHEVMVLDHSGPL
                     LGVILYASAVKLFILGSILMNIVVPVSSGIAWLDWLAAVVELLVMAAAIGVVESIMAR
                     LRMRHVPYLLTAAVMFCGFGFILLVR"
     gene            5715..6356
                     /locus_tag="PLK08_04050"
     CDS             5715..6356
                     /locus_tag="PLK08_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPS52706.1"
                     /translation="MTNILNTVLVILLVMNMLALGTSRLLTVIRIVAMQGVLLGVIPL
                     LVHKHLTIPVMLMAITAIILKAIIIPAVMIRALRSAQIKREVEPLIGLVPSIVLGAIA
                     TVFSLLFADYLPLADAQKGMLLVPAALATVLVGFILLVTRYKAISQVIGYLILENGIF
                     IFSMLLIEAMPFIIEMGVLLDLFVGIFVICIIVNHISQAFSSMDTRRLASLKE"
     gene            6362..>6794
                     /locus_tag="PLK08_04055"
     CDS             6362..>6794
                     /locus_tag="PLK08_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HPS52707.1"
                     /translation="MAFAMILFPLLIAGLAAAVPSNRWRPWLLPATAVAHMVTMLVVL
                     LEPSFADCGKWLKLDPASRVVMLVVDSLFMLCAFYAVGYLHKRQERPNRVFCACMLAF
                     PGIMSMVTWSHHLGLMWVAIEGTTLITAPLIYFNHTPRSIEA"
BASE COUNT         1547 a         1732 c         1789 g         1726 t
ORIGIN      
        1 ttcttaccgg acagatgaac ggtcttattt ccaaatttgc cgatgaatac ggtacattac
       61 gttttgatgt cccggaaggc agttatcgtc tggttttgat gagttcgcgc gttctctacg
      121 acggcacaca tgtcacggaa aacctttcag acccgcgtta ttgcgttaac cttgcagacc
      181 gtgacgccac cagaaaattc atcgaagtga cccatgaaaa atattttgaa gtctgcggtg
      241 aagagttcgg caaaagcata ttggcattct ttactgacga gccgtcactt atagcatggc
      301 acatacggcc aggcactttc ccaatgctgc cgtggcatag ttcatttccg caggaattca
      361 agaaacgtta cggctatcca atcgtcgacg cagtgcttgc cgttttccgc aatgcaggag
      421 aacagacagt caaacgccgc tgcgatttct gggaattcat cgccgatcag gtagccgcca
      481 attattttgg cgaaattcag gaatggtgtc atgcacacgg tttgaaatca tccggccata
      541 tgctatggga agagaaattg tgctggcatg ttcataatta cggcagtttt tatcgttgcg
      601 ccaaacgctt agattggcct ggcatagacc agttgtcctg cgttccgcag caattgatgg
      661 aggctgatta tatacccatt tccagattac tggcatcgat tgccgacatt tctggagaaa
      721 aagagagttt cacggaattt tcagactttg ccgcaacacc ccagaacgta catataagca
      781 tagattggat tcgtgcgacg gtcaactggc atttggcgca agggataaac aacttcacaa
      841 gttaccatct ttggaatcgc tataaagaca cggaaatacg tgaacttaat gattataccg
      901 cgcggctcag ccagataatg catcagggca aacgcgacag ccgcgttgca gtgctgtatc
      961 ctgaagccac catatggaat gccttcaacc cgtcagctgg cggcgatgat gcaaaggaaa
     1021 aaaatgggca catctctcga atcgacaatg tatttgcagt cacctcgtgg gaactgctca
     1081 aaagtcatat tgattttgac tatgtcgatg accagcttct gctcgacgcc aggcttgtca
     1141 acggcaaact tctttacaag aatcgcacct atgaatgcct gatattgcca gccgtagaag
     1201 tactcggtaa agcaattata gacaaaattg tcgcaatgct tcgtgccggc gtaaaggtga
     1261 ttttcgtagg cggcgaaccg gaattgattc gtgacaacgg cgacaagata gactttttat
     1321 ctctgctttc ggaattcgtc tcaaatcagg attttgaacg aatttcggcg acaaatgaaa
     1381 acatgttcaa gaccagtaaa cttctgccgc gaacattccg actgcaaatg aaagactaca
     1441 cagccgccgt cggcgcttat aacataccag ccgataacct tatacgtata cagatgacac
     1501 attcccgcat tctcgacgac ggcacacggc tggtgatggt cgccaacatg ggcggcagca
     1561 gttttattgg caatatagct atcgacacgc cttgcggaga tgttcgcata gctgacccgc
     1621 ataccggaaa aataatcgcc gctaaaacaa ccagacaacc caatcaaact gaaatcgcag
     1681 taacactgcc agcctacgac agtctggttt tcatcatcga ccctgccagg taatggcttt
     1741 tgagcaagaa ctcgcgtcac tgtgcgatta aattggacgt ctgccttatg gcgacgtcgg
     1801 aatgacaaaa gatttggcat caggaaggct ctgccggaat gatgccattt tttttgttag
     1861 aattgttctc gaaagaatgt ttgcaaatac ctgccgatga gacattattg gaattgtatt
     1921 gacatctgta gacataatgt gtaaatttcc cgcttatttt taaaaatgct atggagagtc
     1981 acgaatgcaa ctgtccgtta aagacactgc caggctgttg aatgtttcag aaaaaaccat
     2041 atatcgctgg ataaaacaac aggtcatacc ggtttataag ataaacgagc agttcaggtt
     2101 caaccgttca gaattgcttg agtgggcaac gtccaggcgg atacaggttt cgccggaaat
     2161 ttttaaagag acggagcaga acggcacact cctgccaagt ctggccgacg cattcaaggc
     2221 tggcggaata ttttaccgtg tcggcggcga tgacacgccg agtgtcctgc gagccatcgt
     2281 tgacgtattg aatctgcccg aagaggtcga ccgtgagttt ttataccagg ttctgatggc
     2341 tcgcgaaacg ctgggctcta ccggcatagg tgacgggata gccataccgc acgttcgcaa
     2401 tcctgtggtt ctgcacgtat ccaggccgag catatcgctg tgttttctgg acaatcccat
     2461 agatttttta gccatagacg gccagccggt caatacgttt ttcacgctca taagccccac
     2521 cgtcagagct catcttcata tgctctcacg tctcgggttc gtcctgcaaa acaaggaatt
     2581 caggtccgcg attaaaaagc aggcgccccg cgaagaaata atatcctcgc tcgccgccgc
     2641 cgaagcaacc ctgcccgacg ccgccaactc aaacggagac cagttaaaat gaccgccgac
     2701 gtcataaccc cactgcagta cattatgtta tgacgctgcg gactaaagac atttatccaa
     2761 aaggtttttc atcatatgtc acttacgctt atcacgatag catgtatcat ctctgccgcc
     2821 agcggattgc ccggtctgtt ttcatcacgg ccgtcgtcct ggggtcaaaa gctcgccgcg
     2881 gctatgatgt gtacaagcgc catagtcggc ctcgcaggag cgtttgacgg tttttttgcg
     2941 acatccgtgt ccgaacgttt tttcccatgg ccggcggctg gcaattcttt catcggcatt
     3001 gatgcgctaa gtgcgttttt cttatgtccg gtttttctga tgggcgggct tggagcaatt
     3061 tacgggttgg gctattggcc gcagcgtctg cacatgcgga acgcaagaca acttcagctt
     3121 ttctgggggc tcacgaccgc cggaatggta ctgctcgtaa taagccgtca cgccatggcg
     3181 tttctgctgg gttgggaaat catggcactt tcggcgtttt ttctgatagc tgcggaagat
     3241 gaccgcgaag aatgtcgccg ggccggctgg atttatctca tcgccacgca cgtcggctcg
     3301 ctcgtgttgt tcggcatgtt catgctgtgg cgatgggcta ccggttcata catgtttacg
     3361 ccgacggcag ccggcggtgc tttaagtttg tgcgtgatga atgtgctgtt tttcatgtcg
     3421 ttagtcggct tcggcgtaaa agccggcatc atgccgctgc aattctggct gcctggcgcc
     3481 cacgccaact cgccaagtca cgtctcggca atgatgtcag gcgtagtact gaaaatgggc
     3541 gtttacgggc tggtaagatt tttatggctg tttccgaatc cgccgcatat gtggggcgga
     3601 ctgattttaa tactcggcgc gacaggcggc ctgcttggcg tcgtgtttgc aatcgcccag
     3661 catgacatca aacggctgct ggcatatcac agcgtggaaa atatcggcat tattctgatg
     3721 gggttcgggc tggcgatgct gggccgctca tcaggccgtc aggaatgggt ggtactcggc
     3781 atggccggct gcctgctgca cgtgtggaat cactcgctgt tcaagtcgct gctgtttttc
     3841 agcgcaggct cagtggttca cgcatcgcat acaagggaaa tcgaccgcct gggcggcctg
     3901 gcgaagaaaa tgccctggac ggctgcgatg ttcatgatag gcgcggtggc catatgcggc
     3961 ctgccgccgt tgaacggatt cgtgagtgaa tttttcgttt atgtcggatt gctcaagtca
     4021 ttcgtgggcg acggcgcaac aggcgcggca gccgtaatgg ttgtgccggt actggcgatg
     4081 attggcgcgt tggccgtcgc atgtttcgta aaagtctacg gcgtcgtttt tctgggcaat
     4141 cccagaacgc cggcggcaga acacgtccat gagtcgccgc aaacgatgcg atggcctatg
     4201 gccgtattag ccgcctgctg tttcatcatc ggccttgcac cgataacggt tgttccgatt
     4261 cttaaccgcg cgatagccgt ctggaacttc ggcgccgcac agtcagtcgc aacagccgta
     4321 ccgctaacga cggtaagtac gcttgcaatg gtttttgccg tcgtcattgt tgcgatgctg
     4381 ctgatagcgg ccgtgaaaaa atggcctgca cgccgcagcg tcacctggga ttgcggttat
     4441 gcacagccca cagcccgaat acaatacacc gcctcgtcat tcggccggac gattgtactg
     4501 ctgttcaact gggtgcttcg tcctcgcgaa catcggccgg acatacaggg gctgttcccg
     4561 aaaccgtcga agattcatac gcacgtggac gatatcgttc tggacggcgc aatacttccg
     4621 gctagccggc gtatcagccg atggtttatg tggtttcgca gattccagca gggcattgtg
     4681 cagaactatg tattttacat cctgcttgcc gtaatcgtaa tgctgtgtac gttaatgccg
     4741 ttcggaaaaa tttttacgcg tcttttttcg cgttaattcc ataaaaacgt gagcctgccg
     4801 tgatagaaaa aataatccat attgcaatat tactggttct tccgccgctg ctgttgggtg
     4861 tcatcaaccg gattaaggcg aaattcgccg gccgggtcgg cccgccgctg tttcagacgt
     4921 attacgacat cataaagctc atgcgcaagg acatggtaat aagcgacacg acatcgtggg
     4981 tattcatcgc cgggccggcg ataacgctgg ttgcggtact gctggccggc ctgctgatac
     5041 cgctgggtcg tttcgccgcg ccgataaatt ttaccggcga cctgatactg tttgcctatc
     5101 ttttcgcgtt agccagattt ttcacgacgg cagcggcact ggataccggc tcatcttttg
     5161 aaggcatggg agccgcccgc gaggtgacat tcgcctgcct gtccgaaccc gcaacgtttt
     5221 tcgccatgct ggtactggcg aaagtttccg attcattgac gctgacgaac atgcttcacg
     5281 ggccagccgg cggattttca atagccgtgg cagcaccgct tgtactcata gcgataggcc
     5341 tgttcatcgt actgctggct gaaacgtgca gaattccggt cgacgaccct aatacgcatc
     5401 ttgagctgac aatgattcac gaagtcatgg tgcttgacca cagcggcccg ctgctgggcg
     5461 tgatattgta cgcgtcagcc gtaaagctgt tcattctcgg cagcatactc atgaacatcg
     5521 tcgtgccggt aagcagcggg attgcatggc tggactggct ggcggcggtt gttgaactgc
     5581 tggtcatggc ggcggcaata ggcgtagtcg aatccataat ggctaggctg agaatgcgtc
     5641 atgtgccgta tcttctgacg gcggcggtga tgttctgcgg cttcggtttc atattgttgg
     5701 tgaggtaaac agaaatgacc aatatcctga ataccgttct cgtaatattg ctggttatga
     5761 acatgctcgc cctcggcacg agcaggctac tgaccgtcat ccgcatcgta gcgatgcagg
     5821 gcgtattgct gggcgtcata ccgctgcttg tgcacaaaca tcttacgatt ccggtaatgc
     5881 tgatggcgat tacggcgata atcctgaagg ccataataat tccagccgtt atgattcgcg
     5941 cccttcgcag cgcacagata aaacgggaag tcgagccgct catcggcctt gtaccaagca
     6001 tagtactggg cgcaatagcg acggtttttt cgctgctttt cgccgattat ctgccgctgg
     6061 ccgacgccca gaaaggcatg ctgctggtgc ctgccgcgct ggcaaccgtg ttggttggtt
     6121 tcatactgct cgtcacacgc tataaggcaa tcagccaggt tatcggctat ctcatccttg
     6181 agaacggcat attcattttc agcatgctgc tgatagaggc tatgccgttc atcattgaaa
     6241 tgggcgtact gctcgacctt ttcgtcggca tattcgtcat atgcataata gtcaaccaca
     6301 tcagccaggc gttttcgtcg atggataccc gccggctggc ctcgcttaag gaatgaaccc
     6361 aatggcattc gcaatgattc tgtttcctct gttgatagcc ggcttagccg ccgcagtgcc
     6421 gtcgaatcgt tggcggccgt ggctgctgcc tgcgacggca gtcgcacaca tggtgacaat
     6481 gctagtcgtg ctgcttgagc cttcattcgc agactgcggc aaatggctga aacttgaccc
     6541 ggccagccgt gtcgtgatgc tggtggttga ctcgttgttc atgttgtgcg cattttacgc
     6601 agtcggctat ctgcacaaac gccaggagcg gccgaatcgt gttttctgcg cgtgcatgct
     6661 ggcgtttcca ggcataatga gcatggtgac gtggtcacat catctcggcc tgatgtgggt
     6721 ggctatagaa ggaacgaccc tcataacggc accgctgatt tatttcaatc atacgcctcg
     6781 cagcatagaa gcca
//
LOCUS       DAONFI010000087         6777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13114_length_6777_cov_4.844094, whole genome shotgun sequence.
ACCESSION   DAONFI010000087 DAONFI010000000
VERSION     DAONFI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13114_length_6777_cov_4.844094"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLK08_04060"
     CDS             <1..191
                     /locus_tag="PLK08_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52708.1"
                     /translation="AAKEPIAGSQGFWDRFYNVPEHFKLDMRHFCWLQLRSAASGCIG
                     IRAVFLLRLPCIARFANA"
     gene            complement(248..2548)
                     /locus_tag="PLK08_04065"
     CDS             complement(248..2548)
                     /locus_tag="PLK08_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPS52709.1"
                     /translation="MNDLLEGLNAPQREAVCHMNGPLLVLAGAGSGKTRVITRRVAYL
                     AQQGIDPYNVLAITFTNKAAGEMQQRIAALHTPYGSTVCTFHSLCARLLREFPQQAGL
                     GTNYSIYDRNDQLKIAKKAIERAENVGERITPARAHAAISNAKNAMQTAKAYESQAHD
                     FYTRGISEVYHHYEKILAENNAVDFDDLLMRMAFMLRDHADIREYLSDRFRYILIDEY
                     QDTNRAQYIIAHTLAHRHQNICATGDPDQSIYRWRGADINNILDFEKDYPDAFVIRLE
                     ENYRSRKPILTAAAKLIAHNIDRKEKKLFTRKEGGADVKVILLDDEKAEAQYIADRVE
                     QYRVNGGNYCDVAVFYRVNSQSRVIEDAFMKKGVPYKIARGTEFYNRKEIKDMLAYLK
                     LLANPADDVSCERIINTPPRGIGATTINRLYGYAAANGKSLFDAARNAGSADLSNSAG
                     KKVTVFAELISQLSASLDRPVRIIMEDVFRLSGLETMLSQKDEETRQARANVEELISA
                     AADFDAERPDATLADYLQGVSLVSDTDHWEGSDGAVMLMTLHAAKGLEFPVVFIAGAE
                     EQMLPFTREQLPGEMEDYKEIEEERRLAFVGITRAMEDLTITSARLRTVRGQRQPQQQ
                     SRFLDELGTEFISRHDKTTAIPASSSSHGGFYGGGFSSSLKKKIFDDDWSDFHEAMAR
                     SKQKRHFETATDDNDNNITFEECLDEFPIEYEGLQKGSSVSHPRFGCGKVTRISMPWP
                     DTRVEVMFKDCGLKKLVLLHAKLKVL"
     gene            2779..3666
                     /locus_tag="PLK08_04070"
     CDS             2779..3666
                     /locus_tag="PLK08_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPS52710.1"
                     /translation="MKPILERLKAGEILFVDGAMGTFLYKRGLKPGECHELWCIEHPD
                     VVKDIHMSYRQAGSDIVETNSFGGTRYRLGHYGLAERVREINLAAAKIAREVAGDTQY
                     VLGSAGPTSEFMEPYGSETPEGFYDAFKEQLVALADGGADVCIIETMTCLEEAALAVR
                     AARENTNLTIIASFTFDPKPDGGYVSMMGVTPDVFAPAMLSAGADILGLNCGTGPDHM
                     LNIIKMVKAAAPDAFVCAMPNAGMPVAEDGKLVYRETPAMMASKAVKLAAAGANIIGG
                     CCGTTPEHIAAMKKAVLGR"
     gene            complement(3712..>6777)
                     /locus_tag="PLK08_04075"
     CDS             complement(3712..>6777)
                     /locus_tag="PLK08_04075"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPS52711.1"
                     /translation="NPERYQNGNIILLNQYDQVICSSLHQDWRDSNIRIFEDEITDMV
                     STDAAFKGNPEKQERLARNLAGRLEGEIQRTGEKTSTFSVKGYYVFIQPLKDVPWRFV
                     YVLPAKSVWLAAMGIQEYFFLPLIMVALLLVFIVPHMVFRKYFVKPMIEMVRFMRTSA
                     FHTNAAVPNVPDAWKPWFQEISQLPHLQNIAHSMPGALFQVKLSNDNDEQGHGELGFI
                     SESITAMLDERIESILENLRADVLHYFDETERENLRHESRRIQRYGGNLDMQCRLVTV
                     QGRVRWVRIMATPRVDENGETILEGILLDYTEQNETAKIVQEQQSILQMLTEAAIEGI
                     FINNEETIIQANQALADMFGYSRVEDILGRNINDFMAPESYGTVQEKIKANFTGFYEV
                     KCMRRNGEIFDAEIHVRQTQFGGKKLRAGAIKDISHRKNYELDMKRRDRILESVTQMA
                     EMFITDTDFTNIFDRCFPSLGEAVEVNRITIYRKDETLDQLVLCPEYTWATSKLEKAH
                     ALSLPNIVVPRDGGPIKRWIDKISLGETVYGSSGDFIASEKALLSSYGLRSVAIIPVF
                     VDSEWWGCMMFGVSDRDRDWKTESLEGMKMAGDLFAGAIQRERVMNEIIRTKQRETSI
                     GGIIQDRLLRSVPPKDITDTDISVISVPSEEIDGDFYDFIKYDNTHFDVIIGDVMGKG
                     VSAALLGAAAKAQFMRGVGNFLCTSSLTLLPTPEEVVNYLNNTMCRELISLDSFMTIC
                     YVRFDTDKMRMDYVNCGHPKVIHYHGEQNMCEFLSDENYPIGFDDGEYYQQSSVKFLP
                     GDIFFFYSDGLIESKSPNGELFGAERVSDIIKNNASVTPNELTKKIYENLVSFSMSVS
                     FNDDLTFVAVKILPKTECPLLLSRTKLFPSSVDILKDVRDFIQEQLEEAGKFDKMFIN
                     YVLVASNETTTNIISHGSGQNEIGILVRVDAFMNRAEIRLTYSGGEFVNTLVSSPAFD
                     GSQEHGFGLFMSRQCVDEMIYSEDESQNTRTIKLVKYINPGKNNNSFLK"
BASE COUNT         1485 a         1666 c         1759 g         1867 t
ORIGIN      
        1 aggctgcaaa agagccgatt gcaggcagcc aaggtttttg ggataggttc tataatgttc
       61 cagagcattt taaacttgat atgcgtcatt tctgctggct gcaacttcgc tctgctgcgt
      121 caggctgcat cggaatacgg gcagtattcc tgcttcgcct tccttgcata gctcgtttcg
      181 ccaacgcata aaatgtcgcc tctcaaattt agaatgctct atgcattacg gatttgtttt
      241 gcggctgtca cagaactttg agttttgcat gcagcaggac tagttttttc aggccgcagt
      301 ctttgaacat aacttcaacg cgggtatccg gccatggcat ggaaattctg gttaccttgc
      361 cgcagccgaa acgcggatga gaaacgcttg agcctttctg gagtccctcg tattctatgg
      421 ggaattcgtc caaacattct tcgaaagtta tgttgttgtc gttatcatcg gttgcggttt
      481 caaaatggcg tttctgcttt gagcgggcca tggcttcatg gaagtcgctc cagtcgtcgt
      541 cgaatatttt tttcttgagc gaagagctga atccgccgcc gtagaatccg ccgtggcttg
      601 acgaactggc tggaatggca gtggttttgt catggcgcga tatgaattcc gtgccgagtt
      661 cgtcgagaaa tctggattgt tgctgcggct gacgctggcc gcgcacggtt ctcagccgtg
      721 cggacgttat cgtcaaatcc tccattgccc gcgttatccc tacaaacgca agccggcgtt
      781 cctcttcgat ttccttgtaa tcttccattt cgcctggtag ctgttcccgc gtgaatggca
      841 gcatctgttc ctctgctccg gcgatgaata ccacgggaaa ttccaggcct tttgccgcgt
      901 gcagcgtcat taacatcacc gcgccgtccg agccttccca gtggtctgtg tcgcttacga
      961 gtgaaacgcc ctgcaggtag tcagccagcg tcgcatccgg ccgttcggca tcgaaatcgg
     1021 ctgccgcgga aatgagttct tccacgtttg cacgagcctg ccgtgtttct tcatctttct
     1081 gcgaaagcat tgtttcaagt ccgctcagtc ggaagacgtc ttccatgatt attcgtaccg
     1141 gccggtcaag gctggcagag agttgagaga taagttcggc aaagacggtt acttttttgc
     1201 cggctgagtt tgaaaggtcg gctgagcctg cgtttctggc ggcgtcgaaa agcgatttgc
     1261 cgttagccgc cgcatatccg taaagccggt ttatggttgt tgcgcctatg ccgcgcggcg
     1321 gagtatttat gattcgttcg caactcacgt catccgccgg attagccagc agtttgagat
     1381 atgcgagcat atccttgatt tctttgcggt tgtaaaactc cgtgccgcgg gcgattttat
     1441 atggtacgcc ttttttcatg aaagcgtctt caatcacgcg tgactgcgaa ttgacgcgat
     1501 aaaataccgc aacgtcgcag taattgccgc cgtttacgcg atattgctcg acgcggtcgg
     1561 cgatgtattg ggcttcggct ttttcgtcgt caagcaggat taccttgaca tccgctccgc
     1621 cttcctttct cgtgaacagt ttcttttcct tacggtctat gttatgagct atgagttttg
     1681 cggcggcggt caggatgggt tttcgcgagc gataattttc ctcaaggcgg attacgaagg
     1741 cgtccggata atctttctcg aaatcgagta tgttgtttat gtctgcgcct cgccagcgat
     1801 aaatgctctg gtcagggtcg cctgttgcgc atatgttctg atgcctgtgc gccagggtgt
     1861 gtgcgattat gtactgcgca cggttggtgt cctgatattc gtcgatgaga atgtacctga
     1921 accggtcgga gagatattcg cggatgtcgg cgtggtcgcg gagcatgaat gccatccgca
     1981 tcagcaggtc gtcgaaatcg acggcgttgt tttccgcgag aattttttcg taatggtgat
     2041 agacttctga aatgccgcga gtgtaaaaat catgagcctg cgactcgtag gcttttgcgg
     2101 tctgcatggc gtttttggcg tttgaaatgg cggcgtgagc gcgagccggc gtaatgcgtt
     2161 cacctacatt ctcggcacgc tcgatggctt tcttggctat cttcaactgg tcattgcggt
     2221 catatatgga atagtttgtt ccaaggccgg cctgctgcgg aaattcacga agcagccgcg
     2281 cacaaagcga atgaaacgta cataccgtcg agccataagg cgtatgcagg gctgctatac
     2341 gctgctgcat ttcgccggcg gctttgttgg tgaaagtgat agccaacaca ttgtacgggt
     2401 ctattccctg ttgcgcgaga taggctacgc ggcgggttat gacgcgggtt ttgcccgaac
     2461 cggcgccggc aagcaccagc aacgggccat tcatgtgaca gaccgcctca cgctgtgggg
     2521 cattgaggcc ttcgagtaaa tcgttcattt cgttttcatc tccatccgtg gctttccatc
     2581 ttacaatcta caccaacaaa acgcaaaccg ccattcgtct acaaagggca tttttacgca
     2641 aatcatttgc atcgtcagag aatagttcat ataacatatt tcaggaactg ctataaatat
     2701 gatgaatttc catgtttttg gtataggttc tatatacgtt tacgctgatg gcaacattat
     2761 aaattatcgg agtttttgat gaaaccgata cttgagcggc tcaaagccgg tgagatattg
     2821 ttcgtcgacg gagcgatggg tacgtttctg tataagcgag gcctcaagcc tggcgagtgc
     2881 catgaattgt ggtgcatcga acatccggat gtcgtaaaag acattcacat gtcatatcgt
     2941 caggccggca gtgatattgt cgagacaaat tctttcggcg gcaccagata tcggctggga
     3001 cattacggct tggctgaacg ggtcagggaa ataaacctcg cagcggcaaa aatcgccaga
     3061 gaagtcgcag gcgatacgca gtatgtgctg ggcagcgccg ggcctaccag cgaattcatg
     3121 gagccgtacg gcagcgagac gcctgagggt ttttatgatg cgtttaagga acagcttgtc
     3181 gctcttgcag acggcggagc cgatgtttgc atcatagaga cgatgacttg cctcgaagaa
     3241 gccgccctag ccgtccgtgc cgccagggaa aatacaaatc tcaccattat cgcgtcattt
     3301 acattcgacc ccaaacctga cggcggatac gtcagcatga tgggtgttac gccggacgtt
     3361 tttgcgccgg cgatgttgtc ggctggcgct gatatcctgg gcctgaattg cggaaccggt
     3421 ccggatcaca tgctaaacat tattaaaatg gtaaaggctg ctgcgccgga tgcgtttgtt
     3481 tgcgccatgc cgaacgccgg catgcccgtg gctgaagacg gaaaactcgt ttacagggaa
     3541 acgccggcaa tgatggcgtc gaaagctgta aaattggcgg cggctggagc gaatatcatc
     3601 ggcggctgct gcggcactac gcctgaacat atcgcagcta tgaaaaaggc tgtacttggc
     3661 cgttaaagcg attctcaaaa atgccgcgtg aatggcttac ctgattcgcg attactttaa
     3721 aaaactgtta ttgtttttac cgggattgat gtattttacc agcttgatag ttctggtatt
     3781 ttgggattcg tcttccgagt aaatcatttc atcgacgcac tggcggctca tgaacaggcc
     3841 gaatccgtgt tcctgagagc cgtcaaatgc cggactggaa acgagcgtgt tgacaaattc
     3901 gccgcctgaa tatgtcaggc gtatctcggc acgattcata aatgcgtcaa cccgcaccag
     3961 tatgccgatt tcattttgac cgcttccgtg gctgatgatg ttcgttgtgg tttcattgct
     4021 tgcaacgaga acataattta taaacatttt gtcaaatttg ccggcttctt caagctgttc
     4081 ctggataaaa tcacgtacgt ccttgagtat gtccaccgat gacggaaaca gctttgtcct
     4141 gctcagcaga agcggacatt cggtcttcgg cagaatcttg acggctacga atgtgaggtc
     4201 atcgttgaaa cttaccgaca tggagaatga aacgagattt tcgtaaattt tcttggtaag
     4261 ctcattcggc gtgacggaag cgttgttctt gataatgtca gaaacacgtt ctgcgccgaa
     4321 aagctcgccg ttgggactct tggattcaat aaggccgtcc gagtagaaga aaaatatatc
     4381 gcctggcaag aattttaccg aagactgctg ataatattcg ccgtcgtcaa aacctatggg
     4441 ataattttca tccgaaagga attcgcacat attttgttcg ccgtgatagt gaatgacctt
     4501 tggatggccg cagttgacgt aatccattcg catcttgtcc gtatcgaatc gtacgtagca
     4561 gatagtcatg aagctgtcga ggcttatgag ttcgcggcac atggtgttgt tgaggtaatt
     4621 gaccacttct tccggcgtcg gcagcagcgt caggctgctc gtgcacagga aattgcctac
     4681 gccgcgcatg aattgcgcct tagccgccgc accgagcaac gccgccgata cgcctttgcc
     4741 catgacgtcg cctatgatga cgtcgaaatg cgtattgtca tatttgatga agtcatagaa
     4801 atcaccgtcg atttcttcag acggcacgga tattacggag atatcggtgt ccgttatatc
     4861 ctttggcggc acactgcgca gcagcctgtc ctgaatgatg ccgccgatgc ttgtctcccg
     4921 ctgttttgta cggatgattt cattcattac acgttcgcgc tgaattgcgc cggcgaaaag
     4981 gtcgccagcc attttcatgc cttcgagcga ttccgttttc cagtccctgt ctctgtcgct
     5041 tacgccgaac atcatgcagc cccaccattc actgtcgaca aaaactggaa taattgcgac
     5101 tgaccgcagg ccataactgc tgagcagtgc tttttcggag gctatgaaat cgccggaact
     5161 tccgtagact gtttctccga ggcttatctt gtctatccaa cgcttgatag gcccgccgtc
     5221 gcgcggcact actatgttcg gcagagacag ggcgtgtgcc ttttcgagct tggatgtggc
     5281 ccatgtatat tcaggacaaa gcacgagctg gtcaagagtt tcgtctttac ggtagattgt
     5341 tatgcggttg acctccaccg cttcgccgag cgacgggaaa cagcggtcga aaatgttcgt
     5401 gaaatcggta tcggtgatga acatttccgc catctgcgtg acggattcca atatcctgtc
     5461 acggcgtttc atgtcgagtt cgtaattttt ccggtgcgat atgtccttta tggcgccggc
     5521 acggagtttt ttgccgccga actgggtttg acggacatgt atttcggcat caaatatttc
     5581 gccgttgcgc ctcatgcact tgacttcgta aaagccggta aagttggctt ttattttctc
     5641 ctgtaccgtg ccgtacgatt caggcgccat gaaatcgttg atgtttcttc caagtatatc
     5701 ttcaaccctg ctatagccga acatgtcagc aagagcctgg ttcgcctgaa ttatcgtctc
     5761 ttcattattt atgaaaatgc cttcgatggc ggcttccgtg agcatctgca atatggattg
     5821 ctgttcctgg acaatcttgg ccgtttcgtt ctgctcggta taatccagca gtatgccttc
     5881 gagtatagtt tcgccgtttt catccactcg cggggtagcc attatccgaa cccaacgaac
     5941 gcggccctgc acggtcacaa gacgacactg catgtccaga ttgccgccgt atctttggat
     6001 gcgtctggat tcatgccgca gattttcgcg ctcggtctca tcgaaataat gcagtacgtc
     6061 ggcacgtaga ttttcaagta tcgactcgat acgctcgtcc agcatcgctg tgatgctttc
     6121 gcttatgaag cccagctcgc catggccttg ctcgtcgttg tcattggaga gcttgacctg
     6181 aaaaagcgcg ccaggcatgg aatgggctat gttctgaagg tgcggcagtt gcgatatttc
     6241 ctgaaaccac ggtttccatg catccggcac gttaggaacg gcggcattgg tgtggaaggc
     6301 ggatgtccgc atgaaacgta ccatttcaat catcggcttg acgaaatatt tgcggaatac
     6361 catgtgggga actatgaata ccagcagcag agccaccatt atgagcggca gaaaaaagta
     6421 ttcctggatt cccatcgcgg ccaaccaaac actttttgcc ggcaatacgt aaacaaatcg
     6481 ccatggaacg tccttcagcg gctgaatgaa aacgtaatag ccttttactg aaaatgtgga
     6541 cgttttttcg cccgttcgct ggatttcacc ttccaggcgg cctgccagat ttctggctaa
     6601 tctttcctgt ttttccggat tgcctttgaa cgccgcatcg gtgctgacca tatccgtgat
     6661 ttcatcttca aagatacgta tgttcgaatc tcgccagtcc tgatgcaaag agctgcatat
     6721 cacctgatca tattggttca gcagtatgat gttgccgttt tgatatcttt ccggatt
//
LOCUS       DAONFI010000088         6771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13129_length_6771_cov_3.741811, whole genome shotgun sequence.
ACCESSION   DAONFI010000088 DAONFI010000000
VERSION     DAONFI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13129_length_6771_cov_3.741811"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..935
                     /locus_tag="PLK08_04080"
     CDS             144..935
                     /locus_tag="PLK08_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPS52712.1"
                     /translation="MYISRTMSFDYSKIVDCHCHTELAYCNSGVTAMQAVEDSKKAGL
                     GGVCLTEHAPQLYCTADEFWGVEHINRCIWRNNPASRVPIFKTLVAPYRDDPFVRVGF
                     EVELDADGCLTINDEDRQWADIVVGAIHWLAGEPRDFTQTQFIDAFVDTNRKILAGGV
                     DVLAHPFRVFPSRKRAVDKTLYPLMADMLGEFNTAAEINTHHTSSPDPDFYALCIDRN
                     VKISLGTDSHFPHQVGKIRQNLEVLQKAAGKDDVSEYLYYPGAKK"
     gene            932..1132
                     /gene="thiS"
                     /locus_tag="PLK08_04085"
     CDS             932..1132
                     /gene="thiS"
                     /locus_tag="PLK08_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPS52713.1"
                     /translation="MKLTVNGKSRDAKDGMNIAELLGELKMNPQFVAVEVNRQLVTRK
                     YHSATILKDGDVLEIVTLVGGG"
     gene            complement(1141..1869)
                     /locus_tag="PLK08_04090"
     CDS             complement(1141..1869)
                     /locus_tag="PLK08_04090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPS52714.1"
                     /translation="MIDINLDGKVAIVTGASRGIGRAIAGSLAAAGAQVVLAARSAEL
                     IEEVAVDIESAGGRATPFVTDIADEQSVEALFEMVADDFGRLDVLVNNAGVGIYGPMD
                     EFDLEDLDTILAVNVRGTFACCREALKIMKPQNNGYIINIASVCGEKGYANQSAYSAS
                     KHAIMGLTKSLAAETLEFGIRVTAILPGGVDTEMVAQSRPDLDRSILIKPQDIAYTVM
                     FLLGLNDSCAIDQINIRRRNAKPF"
     gene            complement(1862..2785)
                     /locus_tag="PLK08_04095"
     CDS             complement(1862..2785)
                     /locus_tag="PLK08_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPS52715.1"
                     /translation="MSDIVINNSAENHAGNAHIINKVTWWGLVINVVLSALKMMAGIY
                     GGCQALVADAVHSLSDLATDAAVLIGVKFWSAPADKHHPYGHGRIETLVSLVIGTALF
                     VVAIYIGYEAVVTYKSGEIFRPDWWVFGAAVVSIVSKEWLYRWTVGVGKRIKSTALVA
                     NAWHHRSDAFSSIPVALAVLISMIFPNDIIDHVAALMVSALILKAAWGIIYEAFEQLI
                     DAGADTACVARLRDIAFQIEGVRDVHKIRTRNIGSGLQVDMHVLVDPMMTVLASHDIA
                     AKVQKRLVTSDKDVIDVLIHIEPYEKDGDDD"
     gene            complement(2915..4294)
                     /locus_tag="PLK08_04100"
     CDS             complement(2915..4294)
                     /locus_tag="PLK08_04100"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPS52716.1"
                     /translation="MGMTITEKIMARHSGRTKVQPGENIWCNIDILMTHDVCGPGTIG
                     VFEKEFGKDAKVFDPEKIIIIPDHYIFTADPKAHRNIAILRDFCKRQGIKYFYDPDFI
                     DTDAAGIPAPYADPAKTSYRGVCHSALPQNGHVRPGEILLGTDSHTCTHGAFGQMATG
                     VGNTDAGFVMGTGKLWLTVPSTMKFVFDGELPPYIMAKDIILHVIGEIGFDGATYKAM
                     EFAGDAITSLNVEERCTICNMAIEAGGKSGIIAPDAVTMKYVDARNTGTPYEPVYGDK
                     DAGYECVMNFDASALQPTVAQPHCPDRRALAADLKDVKIQRSYIGSCTGGKTVDFCAA
                     ATVLAGRKVAVDTFIVPATTEVDMDLDRKKVGGRTLREIFIAAGCKIAPASCAACLGG
                     PVDTFGHVHEPINVVSTTNRNFPGRMGHKSAGIFLASPLTAAASAITGHITDPREFCS
                     LTRPIGLAE"
     gene            4528..5175
                     /locus_tag="PLK08_04105"
     CDS             4528..5175
                     /locus_tag="PLK08_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPS52717.1"
                     /translation="MAELEAIRVFLADDHAMVREGIAALVANANDIKIVGQCGNGLEV
                     VNAVKECDPDVIVLDITMPGLNGLDVCRELRRKIDGIEVLVLTMHSDEQLVARAIENG
                     ATGYLLKESAAEQLAEAIRRVAAGEIFLGPGISNKALQQIGRIDNDPYEKLSARERQV
                     LQLIAEGKTNRQIAEILNLSTKTIDTHRTRLMRKLNIHDQTSLVKFAIRQGIISA"
     gene            5276..6118
                     /locus_tag="PLK08_04110"
     CDS             5276..6118
                     /locus_tag="PLK08_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPS52718.1"
                     /translation="MLAINRAVCKNCGLCMVVCPRKVFEKVDGNIEPVNIERCNTCGH
                     CIAVCHSDAITHSELPADSFRDIPQINISAADLKVFLESKRSCRHFRDIEVEKDVIAE
                     IIDVGRFAPSDKNTQRRGFIVVTDRELIKKLDIAVIDEYRKLLKLLPAVSRKVLGTLL
                     PVVRELDIAASGLMRMIQNSDNGESPVFHNAPCVIFTYGPKGVPLAKDTAVATQHYMM
                     LIGQAMGLGSCIIGYAQSRPKTISRILDVPKGQIILSATIFGYPAVKYGKIVPRNKPE
                     VKYF"
     gene            6136..6645
                     /locus_tag="PLK08_04115"
     CDS             6136..6645
                     /locus_tag="PLK08_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPS52719.1"
                     /translation="MEVKDAIYKRRSVRDYNPDVKIPRDALDRIVAAGIEAPSGCNAQ
                     LRHFIIVDNEKIMSQLRMVSSAMKNAPAAIVVVMDPTPTKYGEFWIQDSSAAMENMQL
                     MAVDEGYSSCWIEGPMRQAEPLFRKVLGVPENLRVWAMMPVGKAQTIPGRPQKKTAEE
                     IVSYNKFQQ"
BASE COUNT         1738 a         1787 c         1706 g         1540 t
ORIGIN      
        1 aatatcgccg gctacaaaaa ctttgccttt tacaatcttt tccatcgtat cacctgaaaa
       61 actgttaatt ttcaacaaga cccgattatt gtaacgtcat tgaagcggat ttacaagtcc
      121 ggcttgcacg gaacggaaca tatgtgtata ttagccgcac catgtcgttc gactattcaa
      181 aaatcgtgga ctgtcactgt cacacagagc ttgcctactg caacagcggc gtgacggcaa
      241 tgcaggctgt ggaagactcg aaaaaagcag gcttgggcgg agtctgtctg accgaacacg
      301 caccgcaact ttactgcaca gccgacgaat tctggggcgt ggaacatata aaccgctgca
      361 tctggcgaaa caacccagcc agccgcgtac caatcttcaa aacgctcgtc gcaccatatc
      421 gcgacgaccc gttcgtacgt gtgggattcg aagtggaact cgatgctgac ggctgcctga
      481 cgataaacga cgaagaccgg caatgggcgg acattgtagt cggcgcgatt cactggttag
      541 ccggcgaacc gcgagatttt acacaaacac aattcatcga cgcatttgtg gacacgaatc
      601 gtaaaattct ggctggcggc gtagacgttc tggcgcatcc attccgcgta tttccgtcgc
      661 gaaaacgcgc cgtcgacaag acgctctatc cgctgatggc tgacatgctc ggcgagttca
      721 acaccgcagc agaaatcaac acgcatcaca catcatcgcc ggaccccgat ttttatgccc
      781 tgtgcataga ccgcaacgta aaaatatcgc tgggtacgga ttcgcatttc ccgcatcagg
      841 taggcaaaat acgtcagaac ctggaagttc tccagaaagc cgccggcaaa gacgatgtct
      901 ccgaatatct atattacccc ggagcaaaaa aatgaaactt accgtcaacg gcaaatctcg
      961 cgacgcaaaa gacggcatga atatcgccga acttctcggc gaattgaaga tgaacccgca
     1021 attcgtcgca gtcgaggtca acagacaact cgtcacgcgg aaataccatt cagccacaat
     1081 tctcaaggac ggcgacgttt tggaaatcgt cacgctcgtc ggcggcggtt aagttcgtta
     1141 tcaaaacggt ttggcgtttc tgcggcggat gttaatctgg tcgattgcac aggaatcgtt
     1201 taacccgagc aggaacatga cggtatacgc gatatcctgg ggtttgatga gtatcgagcg
     1261 gtcaaggtcg ggacggcttt gcgcgaccat ttccgtatcc acgccgccag gcaatatggc
     1321 cgtaacgcga atgccgaatt caagagtctc cgcggcaagc gatttcgtca gccccattat
     1381 ggcgtgctta gaagcggaat aggcggactg gttggcatag cctttttcgc cgcaaacact
     1441 ggcgatattg attatataac cgttgttctg cggcttcatg attttcaaag cctcgcggca
     1501 gcatgcaaaa gtgccgcgta cattaacggc taaaatggtg tctaaatctt caaggtcgaa
     1561 ttcgtccatc gggccgtaaa tcccgactcc tgcgttattc accaacacgt ccagccggcc
     1621 gaaatcgtcg gctaccattt caaacagagc ttccacgctc tgttcgtcgg cgatatccgt
     1681 aacaaacggc gttgccctgc cgcccgctga ctcgatatcg acggcaactt cttctatcag
     1741 ttccgccgag cgggccgcga gaacgacctg cgcaccagcc gccgccagcg aaccggctat
     1801 cgccctgccg atgcccctgc tggcacccgt cactatggct accttaccgt caagattgat
     1861 atcaatcatc gtcgccgtcc ttttcatatg gctctatgtg tatcagcacg tcaatgacat
     1921 ctttgtcgga tgtcacaagt cgtttctgca ccttggcggc tatgtcgtgg ctggcaagaa
     1981 cggtcatcat gggatcaacc agcacgtgca tgtccacctg aaggccgctg cctatgttgc
     2041 gtgtgcgaat tttgtgtacg tcacgtacgc cttctatctg aaacgcgata tcgcgcaggc
     2101 gagctacgca ggctgtatcc gcgccggcat ctataagctg ctcgaaggcc tcataaatta
     2161 tgccccacgc agccttcaaa ataagcgcgg aaaccatcaa tgcggcgaca tggtcgataa
     2221 tatcgttggg gaaaatcatg cttatcaaca cagccagcgc aactggtatt gagctgaacg
     2281 catcgctgcg atgatgccac gcattagcca cgagagccgt cgattttatg cgcttcccta
     2341 ctccaaccgt ccagcgatac agccattcct ttgaaactat tgaaacgaca gccgctccaa
     2401 agacccacca gtccggccgg aatatctcgc cgcttttgta tgtcacgacc gcttcgtaac
     2461 ctatgtaaat cgccacgacg aacagcgccg taccaataac cagtgaaacc agcgtctcta
     2521 ttcggccgtg gccgtaaggg tgatgtttat ccgccggcgc agaccagaac ttcactccaa
     2581 tgagaacggc ggcatcagta gcaagatcgc tgaggctgtg aacggcgtca gccaccaacg
     2641 cctgacagcc gccataaatg ccggccatca tcttcaaagc actgagaacc acattgatta
     2701 caagccccca ccacgtgact ttattgatga tgtgggcgtt tcctgcgtgg ttctcagccg
     2761 aattattgat gacgatatct gacatgacag aattttatat tcgtaactat cactttgcca
     2821 gataattatt tcattcaaaa aagcagcccg acgacacaca tagcgcggtt gaacggcgac
     2881 aaataaccgc cggcatcgat tcggcataaa aaaattattc agccaagcct atcggcctgg
     2941 tgagactgca aaattcgcga gggtcggtta tgtggccggt gatagcactt gccgccgcag
     3001 taagcgggct ggccagaaat atgccggcac tcttgtggcc cattctgccc ggaaaattgc
     3061 ggttagtcgt cgaaaccaca ttgattggtt cgtgtacatg gccgaacgta tccaccggcc
     3121 cgccaaggca ggctgcacag ctggcaggcg cgattttgca tccagccgca ataaatatct
     3181 cacgcaacgt cctgccgcca acttttttac ggtcaaggtc catgtcaact tcagtagttg
     3241 cgggaacgat aaaagtatcc acggcaacct tccggccggc cagaacggta gctgcggcac
     3301 agaagtccac cgtcttgccg ccggtgcacg aaccgatata actccgctga atcttcacat
     3361 ccttgaggtc agccgccagc gcacgtctgt ctggacaatg cggctgagcc acagtaggct
     3421 gaagcgcgga ggcgtcaaaa ttcattacgc attcatatcc ggcgtccttg tcgccgtaaa
     3481 caggctcata cggcgtaccg gtgttacggg cgtcgacata tttcatggtg acggcatcgg
     3541 gtgcgattat gccgctcttg ccgccggctt cgatggccat attgcatatc gtacaacgtt
     3601 cttcgacatt cagactggtt atggcatcgc cggcgaactc catcgcctta tatgtcgcac
     3661 cgtcaaagcc tatctcgccg ataacgtgca gaatgatatc cttggccatg atatacggcg
     3721 gcaattcgcc gtcaaacacg aatttcatcg tcgacggcac ggtcagccac agcttgcccg
     3781 ttcccatgac gaaccctgca tccgtattgc cgacacccgt agccatctgg ccgaaagcgc
     3841 catgcgtgca cgtatgcgaa tccgtgccga gcaggatttc gcctggccga acatggccgt
     3901 tctgcggcag cgctgaatga catacgccgc gatacgacgt cttcgccgga tcggcatacg
     3961 gcgccggtat gccggcagcg tcggtgtcga tgaagtccgg gtcgtaaaaa tattttatgc
     4021 cctgccgttt gcagaaatca cgcaatatgg caatgttgcg atgagccttc gggtcagcgg
     4081 taaaaatata atgatcaggt atgattatga ttttttcagg gtcaaagact ttggcatctt
     4141 tcccgaactc tttttcaaac acaccgattg tgcccggccc acagacgtca tgcgtcatca
     4201 gaatatcgat attgcaccag atattctcgc caggctgaac tttcgttctg cctgagtgac
     4261 gggccattat tttttccgta attgtcatac ccatgctggc gtactccttc aaaaagatga
     4321 tattttacac aattacagct ttttatgcaa taagcatgcc tggttatgac cgttttaatc
     4381 accataatgt tcatgttttt caaatacagc tataaatctt cttgttttag aggattaggg
     4441 ttgatattat tgttatatgt agaagtaaag ggcgagtcgt tcgtagcctt atattattcg
     4501 gtaacccgct tatcagggga gattaaagtg gcagaactgg aagcgatacg agtttttctt
     4561 gcagatgacc atgctatggt cagagaaggt atagcagcgt tggttgccaa tgccaatgac
     4621 atcaaaatcg tggggcagtg cggaaacggg ctggaagttg tcaatgccgt aaaggagtgc
     4681 gaccctgatg ttatagtatt ggacatcaca atgccggggc ttaacgggct ggacgtttgc
     4741 agggaactca ggcgaaaaat tgacggaatc gaagtgctgg tgctgaccat gcacagtgat
     4801 gagcaactcg tggcaagggc aatagaaaac ggcgctacgg gatatctttt gaaggaatcg
     4861 gcggctgagc agttggccga agccatccga cgggtggcag ccggcgaaat attcctcggc
     4921 cctggcatat cgaacaaggc tcttcagcag attggacgaa ttgataacga tccgtatgaa
     4981 aaactttcgg caagagagcg gcaggttctg caactcatcg ccgagggaaa aacaaaccgg
     5041 cagatcgccg agattctcaa tctctcgaca aagaccattg atacccacag aacaaggctg
     5101 atgcggaagc tgaacattca cgaccagact tcactggtga aattcgccat acgacagggt
     5161 ataatatcgg cttgattcga cagggttgta cgaaatttaa ctccgtcagg atttgccggc
     5221 gttaaccggc tggcgtcgtg gtgtattttg cgcaaaaaat tcagaggatt tgacgatgtt
     5281 ggcaatcaac cgagcagtct gtaaaaattg cggcctttgc atggtcgtct gcccgcggaa
     5341 agttttcgaa aaagtcgacg gcaatatcga gccggtcaac atcgaaagat gcaacacctg
     5401 cggccattgc atcgcggtat gtcacagcga cgccataacc cattccgagc ttccggctga
     5461 ttcttttcgc gatattcctc agataaacat ttcggcggct gacctgaagg tttttctgga
     5521 atccaagcga agttgccggc atttcagaga cattgaggtt gaaaaagatg tcatagctga
     5581 aatcatagat gtcggccgtt ttgcgccaag cgataaaaat acgcaacgcc gcggctttat
     5641 agtggtaacc gaccgtgaac tcataaaaaa gctcgacatc gcagtcatag atgaatatcg
     5701 caaactcctg aaacttctgc cagccgtcag ccgtaaagtt ttgggtacat tgctgccggt
     5761 cgtccgcgaa ctggacatcg ccgcgtcagg cctcatgcga atgattcaaa attccgacaa
     5821 cggcgaatcg ccggtatttc acaacgcacc gtgcgtaata ttcacctatg gccccaaagg
     5881 cgtgccgctc gcaaaagaca ccgccgtcgc aacgcagcat tacatgatgc tcatcggcca
     5941 ggcgatgggg cttggcagtt gcatcatcgg ctatgctcaa tcacggccca aaaccatcag
     6001 tcgcattctg gacgtgccca aaggccagat tatcctgtcg gcgacgattt tcggatatcc
     6061 ggcggtcaaa tacgggaaaa tcgtcccgcg aaataagccg gaagtgaaat atttctgaac
     6121 gcatggagaa tgaacatgga agtaaaagac gcaatttaca aacgccgcag cgtccgcgat
     6181 tataatccgg atgtcaaaat tccgcgcgat gcgcttgacc gtatcgtagc ggcgggcatc
     6241 gaagcaccga gcggctgcaa tgcgcaactc cgccatttca tcatcgtgga taacgaaaaa
     6301 atcatgtcgc agctgcgtat ggtgagttca gctatgaaaa acgcacccgc ggcaatcgtg
     6361 gtcgtgatgg acccgacgcc tacgaaatac ggcgagttct ggattcagga ttcatcggct
     6421 gccatggaga acatgcagct catggctgtc gacgaagggt attcgtcgtg ctggatagaa
     6481 ggcccgatgc gtcaggcaga gcccctgttc cggaaagttc tcggcgtgcc ggaaaacctg
     6541 cgggtctggg ctatgatgcc ggtcggaaaa gcccaaacca ttccgggccg cccacagaaa
     6601 aaaacggctg aagagatagt ctcatataat aaatttcaac aataacgaag tttgagaacg
     6661 acctctagag catgttaaat ttgaaattgc gtcttgttgc gtcgttgagg acaaggctga
     6721 caaggaaggc gaagcaggca acctgcatgg tttccgatgc agacggacaa t
//
LOCUS       DAONFI010000089         6757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13170_length_6757_cov_4.318562, whole genome shotgun sequence.
ACCESSION   DAONFI010000089 DAONFI010000000
VERSION     DAONFI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13170_length_6757_cov_4.318562"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLK08_04120"
     CDS             complement(<1..472)
                     /locus_tag="PLK08_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52720.1"
                     /translation="MRRNLLVLVLAAAVMTLAVGCGSGYNAAEIAQSYGILNRCDLQP
                     KIASIIQSGEADKMIDTGSAAVRYRVQNCGSNRLLVTEYLIQGEVYRLEVRKAFNPLK
                     NVPADSIRYAYYDKMLDRDYICWFQLSSVINSLQNNSFSAEYVVAYRAWLDLNGK"
     gene            complement(664..1128)
                     /locus_tag="PLK08_04125"
     CDS             complement(664..1128)
                     /locus_tag="PLK08_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52721.1"
                     /translation="MNDRQPKDIVTAASAALNGESPQKFLAEIQETLVAAQREIDACN
                     PQCDACGRCCLFETAGHRLYVSTGELALLLSGEIPPKNGPLHCRWQISDRCTVRETRP
                     LGCRVFYCKTDLCDIYEKFHRKLVNIHKKYGISYFYAEMTAAAEEFYSTHRH"
     gene            complement(1121..1552)
                     /locus_tag="PLK08_04130"
     CDS             complement(1121..1552)
                     /locus_tag="PLK08_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPS52722.1"
                     /translation="MKDFSNNEIPWYVAGLAFECQGCGRCCSGPEEGYVWATPSELDI
                     IAGSLNMTPEQFAQKYVRKVGRRLSLIEDKKTKDCIFLKKGRCTIYEVRPTQCRTWPF
                     WQSNIDSPDDWAWAGKRCAGINRGKKHNAREIENLAKSTNE"
     gene            1719..3338
                     /gene="xrt"
                     /locus_tag="PLK08_04135"
     CDS             1719..3338
                     /gene="xrt"
                     /locus_tag="PLK08_04135"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HPS52723.1"
                     /translation="MIAPAAGLLLLLGLVCNFGFNFFEMWLRWFPAWSRTDLNFYRRL
                     VEGESYYTHGPLILLLCVLMAWLMVHYTKIEIRLSRSAKIVGWCGFGVFMSVHMAACL
                     ARVNFISGFALIGLLLSLVLLIWGWRALRRLWFIPVFMAFMIPLPEVTIAQVNFRLKM
                     MAADTGVHLADFLGVVVFRDGNKVFIEGGKTLVIANVCNGLRTIISLLAFGAMYAYVC
                     RLRGIWRWFLFLMAVPVALLANTLRILSLIIVADLTTAEFAAGWYHDVSSLLIFVLAF
                     LLLFLVEQCVIWLFRATGRPLKVVPLFADSRRTDMNDGQIHRFCDSLSSWRSWIAGVL
                     LVLFSIEAGLLNRAEPPKATPAMLDSAMPMTFDVNSMRYVGYPVEIDRQTLTILENPG
                     YLSRQYSCPDNAGDIEVTVFFSQDNRKGIHPPDLCLEGGGDSIIGKNVVQAGDVPCRA
                     LLFVHEEQLYYMLYTYKCGRSYTLSFWRQQLTIFLNGLLERNSTGALVRVATPVIVGD
                     GMDKATAIRQAASRAEGMMTNIIPCIGKAFD"
     gene            3364..>6757
                     /locus_tag="PLK08_04140"
     CDS             3364..>6757
                     /locus_tag="PLK08_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS52724.1"
                     /translation="MKKVLIIVLLILTGLVIGGVAWWRVRAGRPDRMLSRAQTALNAS
                     MLDDAMALAARYAERFPDDWHGYFIKAQVLMRRGQYAEARETLARAVELLKNNVAAGD
                     AGDTKNITRIEMVMANSYSFPASYVMAKRSDDVSEKQLQDAAENIKKSLKILGDIKEA
                     DIGLHLDILQAAGLDSRLLGECYGRIAGKLRAQARLRQSGGFEKRYETIMRSAADYDR
                     LAAAAYTEAMNKLYDVVSAEPTRKTAGQNLAELCIISGDIQPYEKARKLLLEEKKVVP
                     DAKVILWMQDIQKNQTVLTPPQFASKLSKTISQVDELLKNNPDNRRLLIARAQLAILQ
                     KDGETAQKCCEKVLASQPRHDQARLLLALAMIQKGQKKEAEDILYELKTDFPNWPDAQ
                     YSYATVENLMGKGELAKQALRHTVQIDPGHAGARRMLVEMMMKEGFYNNAYMDARAYY
                     EAHPDDPKALELFVASAMRSDRRKVALEALDAAIKKYPDNPAMMLVAANGYSMLGESA
                     TARDVIRPAADATVGSWQERLAVVRAKMMAGQITQAENMLLDAAKSAPNSAVVYQELG
                     NLYAATNRPMPAIENYRKSLSLDETNQFCRMSLAKMLFQTGLLKDALAEVQKVLDVEP
                     TNNQAAILADQIRVILGIPTEAVASDGSQNVESESLLKLAMTFLEHGKPEKAIELCDR
                     VLRNSPASAAAFLTVAQARLEQGRLQDAIDNCERAISCDPELLPTYLRMAVILSKKLT
                     PEKVGDIFSKAASAKIYMEQMAMGWLYEQQGDWARAQNFYGRVIYDDDTPKLIKNMAL
                     LRKAIVQGLQGRVDEAFATLSELEKNPQWAQQALIVKAQVAVQNGRSCLDSLKKLLTI
                     ADEKDDPTTFEAVVLMAVQAGERDFAAKVCDVYRQASRQSARPFIMMGLVDVSAGRYV
                     QAAEAYEQAVKIQPSNVQTRIMLAGVYNQCVMPQKALQTLDEMTPLSRSAKAQALYEK
                     AKLLTSWGLQQPAIAALGELADMGFGDDPRVQFAMGVAYAALGRISSARDSLLAVPSY
                     VPQYMPARQLVADLEISGDEKLRILNELLAANPDIDGVRLQKIRTLMAMDRNDSAAAE
                     ANGMLKKFESYGSLPANVADDLLSVLLKLELYPQSLHLAQRQE"
BASE COUNT         1564 a         1669 c         1928 g         1596 t
ORIGIN      
        1 ccttgccgtt gaggtcgagc cacgcgcgat aggctaccac gtattcagct gaaaagctgt
       61 tattctgcaa cgaattgatt acgctggaga gttgaaacca gcagatgtag tccctgtcga
      121 gcattttgtc gtaatatgca tagcggatgg agtcagccgg cacgttcttg agcggattga
      181 atgccttacg cacttcaaga cggtaaactt caccctggat gagatattct gtcaccagca
      241 gcctgttgct gccgcagttc tggacgcgat atcgaacagc cgctgagccg gtgtctatca
      301 tcttgtcggc ttcgccggac tggatgatgc tggctatctt cggctgaaga tcgcaacgat
      361 tgagaatgcc gtaactttga gcgatttcag cagcattata tccgcttccg caaccgactg
      421 ccaacgtcat aactgctgct gccagtacca gaacgagtaa attccttcgc atgcctgaag
      481 tctccgcatt tcaatgaaaa aacattacta cgcagagtct cattacgcat actctgcggc
      541 tacgatacac caaagattat caattcgcca aacgtctgtc aaacgaaaac ttgcgtcaaa
      601 acagcccgaa gtgcattaaa gttatgcgtc aagtttgaaa tgaagaataa ttttgaaaac
      661 cgctcagtgt cggtgtgttg agtaaaactc ttctgcggcg gcagtcattt cggcataaaa
      721 atagcttatg ccgtattttt tgtggatatt tacgagtttt cgatggaatt tttcatagat
      781 gtcgcataaa tcagttttgc agtagaacac tctgcagccc agcggccgtg tttcgcggac
      841 ggtgcatctg tcggatatct gccagcggca gtgaagcggg ccgtttttgg gcgggatttc
      901 ccctgatagc agcagtgcca gttcgcctgt tgagacgtat aatctgtggc cggcggtttc
      961 aaacaggcaa caacggccgc aggcgtcgca ttgcggattg catgcgtcta tttcgcgctg
     1021 ggctgcgacg agcgtttctt ggatttccgc cagaaatttt tggggcgatt cgccgttcag
     1081 ggctgctgac gcggcagtga cgatatcttt gggttgacgg tcattcattc gttgattttg
     1141 ccagattttc tatttcgcgg gcattgtgtt ttttgccgcg gtttatgccc gcgcagcgtt
     1201 ttcccgccca tgcccagtcg tcgggcgaat cgatattgct ctgccagaat ggccacgtgc
     1261 gacattgcgt gggccttacc tcatatatgg tacatctgcc ttttttgagg aaaatgcagt
     1321 ctttggtctt tttgtcttct atgaggctta gccgtctgcc gaccttgcgg acgtattttt
     1381 gggcaaattg ctccggcgtc atgttcagcg agccggcgat gatgtccagt tcacttgggg
     1441 tagcccatac gtaaccttct tccggcccgc tgcaacagcg gccgcagccc tgacactcga
     1501 aggctaatcc ggctacgtac cacggtatct cgttgtttga aaaatctttc atgccacgat
     1561 tatatgaaaa atgaagctga atgcaaaaat aaccgttgcg ggacgaaagg cggtttcgta
     1621 aatttcctgc ctgtaggggg tttatattga atggctggcg atgtataatc ccgtgcatgg
     1681 agttgcgaac aaaagaagga gttcccggaa gaagcagatt gatagcgccg gcggcaggtc
     1741 tgttgttgtt gctggggctg gtctgtaact tcggattcaa ttttttcgaa atgtggctga
     1801 ggtggtttcc agcctggagc cgcaccgatc tgaacttcta tcgccgcctc gtcgagggcg
     1861 aaagctatta tacgcacggg ccgcttatcc tgttgctgtg cgtgctgatg gcgtggctga
     1921 tggtacatta tacgaagata gaaatccggc tgagccgttc ggctaaaatc gtcggctggt
     1981 gcgggtttgg cgtgttcatg tccgtacata tggcggcttg cctggcgagg gtgaatttca
     2041 tcagcgggtt tgcgttaatc ggccttttgc tgtcgctggt attgctcata tggggctggc
     2101 gggcgcttcg gcggctgtgg ttcataccgg tattcatggc gttcatgatt cctctgccgg
     2161 aggtcacgat agcgcaggtt aatttccgcc ttaaaatgat ggctgccgat acgggagttc
     2221 atctggcgga ctttttgggc gtggtggttt ttcgcgacgg caacaaggta ttcatcgaag
     2281 gaggcaagac gctggtaatc gccaacgtct gtaacggctt gcggacgatt atcagcctgc
     2341 tggccttcgg cgcgatgtac gcctatgtct gccgcctgcg tggaatatgg cgatggtttc
     2401 tattcctgat ggctgtgccg gtggcgttgc ttgccaacac gttacgcata ctcagcctta
     2461 taatagtggc agaccttact actgccgaat tcgccgccgg ctggtatcat gacgtttcaa
     2521 gtctgctgat attcgtcctg gcgttcctgt tgctgttcct ggtggagcag tgcgtcatat
     2581 ggctgtttcg cgctacgggc cggccgctca aggttgtgcc gctgtttgcg gattcgcgtc
     2641 gcacggacat gaacgacggc cagattcaca ggttctgcga ctcgctgtca agctggcgaa
     2701 gctggatagc cggcgtattg ctggtgctgt tttccataga ggctggcctt cttaaccgcg
     2761 ccgagccgcc gaaggctacg ccggcaatgc tcgattctgc catgcccatg acgtttgacg
     2821 tcaactcgat gcggtatgtc ggttatccgg ttgaaatcga caggcagact ctgacgatac
     2881 tggaaaatcc aggctatctg agccgccagt attcctgccc cgacaatgcc ggcgatatcg
     2941 aggtgaccgt atttttcagc caggacaatc gcaaaggcat acatccgccg gatctctgcc
     3001 ttgaaggcgg cggcgacagt attataggca aaaacgttgt gcaggctggc gacgttccat
     3061 gcagggcgtt gctgtttgtg cacgaagagc agttgtatta catgctttat acgtacaaat
     3121 gcggcaggag ttatacgttg agcttctggc ggcagcagtt gacgatattt ctcaacggat
     3181 tgctggagag aaattccacc ggcgccctgg taagagtggc tacgccggta atcgtcggcg
     3241 acggaatgga caaagcgacc gcaatcaggc aggctgcttc gcgtgcggaa ggcatgatga
     3301 cgaatataat tccatgcatc ggaaaagcct tcgactgaag gcgattttgc cggagtatca
     3361 gagatgaaaa aagttctgat aatagttctt ctcatactga cgggtcttgt tatcggcggc
     3421 gtagcctggt ggcgggtgcg tgccggccgt ccagacagga tgttgtcccg tgcgcagacc
     3481 gcccttaacg cttccatgct cgacgacgcg atggctctgg cggccagata tgccgaaaga
     3541 tttccggatg actggcacgg gtatttcatc aaggcccagg tattgatgcg gcgcggccag
     3601 tatgccgagg ctcgtgaaac gcttgcccgt gccgtcgagc tgcttaaaaa caacgtcgct
     3661 gccggcgatg ctggagatac aaaaaacatc acgcgcatag aaatggtgat ggccaattca
     3721 tattcgtttc cagccagtta tgtgatggcc aagcggtctg acgacgttag tgaaaagcag
     3781 ttacaggatg ctgcggaaaa tatcaagaaa tccctaaaaa tactcggcga cataaaggaa
     3841 gccgacatag gtttgcatct ggacattttg caggcggcag gccttgattc gcggctgctt
     3901 ggcgaatgct acggcaggat agccggaaaa ttgcgggcgc aggcaaggct cagacagtcc
     3961 ggcggatttg aaaaacgcta tgagaccata atgcgtagcg cggctgatta tgacaggctt
     4021 gcggcggcgg cgtacaccga agcaatgaac aaactttacg atgtcgtctc cgccgagcct
     4081 accagaaaga cggctggcca gaatctcgcc gaactctgca taatcagcgg cgatattcag
     4141 ccgtatgaaa aagcccgaaa actcctgctg gaagaaaaaa aagtcgtccc tgacgccaag
     4201 gtgatactat ggatgcagga tatacagaag aatcagaccg tccttacgcc gccgcagttt
     4261 gcgtcgaaat tatccaaaac catttcgcag gttgacgaac tgcttaaaaa taatcctgac
     4321 aaccgccgtc tgcttattgc acgtgcgcag ctggcgattc ttcaaaaaga cggcgagacg
     4381 gctcaaaagt gctgcgaaaa agttctggcc agccagccca ggcatgacca ggctcgtctt
     4441 ctgcttgcgt tggccatgat tcagaaaggc cagaaaaaag aggctgaaga catactctac
     4501 gaactcaaga ccgatttccc taattggccg gacgctcaat acagttatgc gaccgttgag
     4561 aatctcatgg gcaagggcga gttggccaag caggctcttc ggcataccgt tcagatagat
     4621 cccggccatg ccggcgcaag acgaatgctg gttgagatga tgatgaagga gggtttttac
     4681 aacaacgcat acatggatgc cagagcctac tacgaggctc atcccgacga ccccaaagcc
     4741 ctggaactgt tcgtcgcttc cgccatgaga agcgaccggc gaaaagttgc actggaagcc
     4801 ctcgatgccg cgattaaaaa atatcccgat aatccggcca tgatgcttgt ggcggctaac
     4861 gggtattcaa tgttgggcga gtcggctacc gccagggacg taataaggcc tgcggctgac
     4921 gcaacagtcg ggtcgtggca ggaacgcctc gccgtggttc gcgccaagat gatggccggc
     4981 cagataacgc aggctgaaaa catgcttctg gatgccgcga agagtgcgcc gaattcagcc
     5041 gtcgtctatc aggaactggg caatctctac gcagccacga atcggccaat gccggcgata
     5101 gaaaattatc gcaagtcgct ttcgctggat gaaacgaacc agttctgccg aatgtcgctg
     5161 gcgaagatgc ttttccagac cggtctgctc aaagatgcgc tggctgaagt gcagaaggtg
     5221 ttggacgtag agccgacgaa caatcaggcg gccatactgg ctgaccagat acgtgtaata
     5281 ttgggcattc cgacggaagc ggtggcgtca gacggcagcc agaacgtcga aagcgaaagc
     5341 cttctgaagt tggccatgac ttttctcgaa cacggcaagc cggaaaaggc tatcgaactc
     5401 tgcgaccggg tgttgcgaaa ttcacctgcc agtgcggcgg cttttctcac cgtagcgcag
     5461 gcgcggctgg aacagggcag gctgcaggac gcaatcgaca actgcgaacg tgccataagc
     5521 tgcgaccctg aactgcttcc gacgtatctt cggatggctg tcattctttc taaaaagttg
     5581 acgccggaaa aggtcggcga catcttttca aaagccgctt cagcgaagat ttatatggaa
     5641 cagatggcta tgggctggct gtacgagcag cagggcgact gggccagggc tcagaatttt
     5701 tacggcagag taatttatga cgacgatacc ccgaaactta ttaaaaacat ggccttactc
     5761 aggaaggcta tcgtgcaggg tcttcagggc agggtggatg aagcatttgc gacgttgagt
     5821 gaactggaga aaaatcctca atgggctcag caggcgttga tagtgaaggc acaggtggcg
     5881 gttcagaacg gcaggtcatg cctggattcg ctgaaaaaac ttttgacgat agccgatgag
     5941 aaagatgacc cgacaacttt tgaagccgtc gttctgatgg ctgtgcaggc tggcgaacgt
     6001 gactttgcgg caaaagtctg cgacgtctat cggcaagcca gccgacagag tgcgcggcct
     6061 ttcataatga tgggactggt cgatgtctct gccggccgat atgttcaggc ggctgaggct
     6121 tacgagcagg cggtcaaaat tcagccttcg aacgtgcaga cgcgaatcat gctggccggc
     6181 gtttacaatc agtgcgtcat gccgcaaaaa gccctgcaaa cactggatga aatgacgccg
     6241 ctcagccgga gcgcaaaagc gcaggcactg tatgaaaagg ccaaactgct tacaagctgg
     6301 gggcttcagc agcctgctat cgcggcgctc ggcgaactgg cggatatggg tttcggcgac
     6361 gacccgcgag ttcaattcgc catgggagtt gcgtatgctg ccctgggcag gatatcttct
     6421 gctcgcgatt cgttattggc cgttccgtcc tacgttccgc aatacatgcc agccaggcaa
     6481 ctggtggctg acctggaaat atccggcgac gagaaattgc ggatattgaa cgaacttctg
     6541 gcggctaatc ccgatattga cggtgtgcgg ctgcagaaga tacgcacgtt gatggctatg
     6601 gacaggaatg actcagcagc cgcggaagcg aacggcatgc tgaaaaaatt cgagtcatac
     6661 gggtctctgc cggcaaacgt ggctgacgac ttgctcagcg tgcttctgaa gctggagctt
     6721 tatccgcaat cattgcattt ggctcagcgt caggaac
//
LOCUS       DAONFI010000090         6726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13270_length_6726_cov_4.828212, whole genome shotgun sequence.
ACCESSION   DAONFI010000090 DAONFI010000000
VERSION     DAONFI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13270_length_6726_cov_4.828212"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLK08_04145"
     CDS             complement(<1..219)
                     /locus_tag="PLK08_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52725.1"
                     /translation="MRFVSRVSAVLLVAVIVAGCVQVNAPKQVDVNLNDNTKSGNLSA
                     GSLPEMSQNGGGSVISQAATAAKSMADSD"
     gene            complement(292..477)
                     /locus_tag="PLK08_04150"
     CDS             complement(292..477)
                     /locus_tag="PLK08_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52726.1"
                     /translation="MRFILISVVLGCFAIGCFQLNAPKEVNVNVGGEKKETVKYSTDK
                     EYWKSYGKSFTSSDKED"
     gene            complement(502..2244)
                     /locus_tag="PLK08_04155"
     CDS             complement(502..2244)
                     /locus_tag="PLK08_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52727.1"
                     /translation="MFYAIWNILILSVGCFVCTAAGAAVFRRLTGDEFLKKNFDRVET
                     LCAEFLCGTGILGTAWLILAILPGGGWLGRLIVMLACIVAVMLGGRSAAKDVNLAWRH
                     IKIIVTHPYRESDISVKILHWLILAGLAAMLVASFFPPDNYETAGKFLVAKTAACYGG
                     ITGGAAGGVELFAAGGMLAEMNIAALMLFGGAMAAGVVGFIYLLAGMMLASGIGRRAG
                     LNYLDRWLLVLVLTAWGVAVSRCGNFDAYASGGLVIVALVTLSVADGSRRGVHILAGL
                     MIAWAIMMQYMQAVVILPATAAFAFSLFSRQRQNQANNADEANLQPTVMRRMGWLFSP
                     VGAMLILWLLKNIAVYGSLLPVQPDNPFNAATMLSVATLPLMLLLVIFLRWSAGRKMW
                     IGKIDCGRAISFLWQLLAFIIVWIGIGDMTSLNRNIFAAMNPSGEKGTDFICMNDNAD
                     ATKDRVFTLSPYIYFMDERFLKSSAHRELLRELCDKDDTEKWRELWAKNFRLFYLTNE
                     NTVFSRDGKEFRFSVILDDGRVFTLDSAKLPPELGLERLVVNGSGKSCTSCGYKLVIR
                     ENAIIPDGISTIEN"
     gene            complement(2308..3444)
                     /gene="ispH"
                     /locus_tag="PLK08_04160"
     CDS             complement(2308..3444)
                     /gene="ispH"
                     /locus_tag="PLK08_04160"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPS52728.1"
                     /translation="MKDPLEAFHSTIIDDIRSAHTPNVWKLPGGELRLPEVFGFCRGV
                     KRALMMAVEAVENSRRNNDTSRLILLGEIIHNPWVNDYFSEHGVKILSAEERRCDRIE
                     KHVKSGDTVIIPAFGVALPIEKRLREIGCRIIDCSCGDVIRLWRWSEKAVAGGYSILI
                     FGRSLHDETVVTKSRLEAAGGKYLVVENLNQVRLFCDMFAGKSDEAFSVFPPHATNAG
                     DIDSFMRLAQVSQTTMLYNDTMKVQAMLKAAFDVRPCASREDLAFQPTVCKATQARQN
                     ASVELCKSGCDIVIVVGGFTSSNTRHLYELARQFAPSYLIEDNSAIHNENELLAWNAE
                     TSEPAVIKNWLPAMRPLKIGILAGASSPEIVVGRVLTRLAGFLG"
     gene            complement(3446..5266)
                     /locus_tag="PLK08_04165"
     CDS             complement(3446..5266)
                     /locus_tag="PLK08_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52729.1"
                     /translation="MQTEIKELGEFRDTMRRFVGSYRELPVSTRATCPKCHRVVPAEF
                     RRHKSQVVLAFNCPHCGEQPPIVHDDAIWTNRASDLPGSATETFSGCRIRPVSRRLPR
                     TVETLCPECGAIIPGRYFVQDSAVWIEKTCPDHGYFRDRINSDVKLFAKASWWSFEEH
                     AGLEFPHVTDGKNCPSDCGLCNQHLSGPCLAQIDLTNRCNMKCPICFANANAQGYIWE
                     PDYNEIVRELKVLRAMKPHPTTAIQFTGGEPTIHPDFLKIISAARDMGFSYIQIATNG
                     ITLSDENFARQCRDAGLHTLYLQFDGVGDEAYKETRNYPGIWSKKLAAIENARKVGMK
                     VCLVPTIVKNINDEQVPKILDFAFRNVDVISGISWQPVSFTGRMSVEELQTHRYTLGD
                     LAHDMAKCSNVVPMRDIMPLSIVVPLSNMLEAITDNPKVRPSCHPDCAMGTYFIVSPS
                     GQAWPFPAVLDVEGLFAGMNVLANKIKAAGRTANFFDKLRIVWLFMKNWRWKTAPKDI
                     KISLLMRSIMGMVDKRIGRGKGSEKNYRTLLSAGMHFQDRYNFDVERVKRCVILYATP
                     AGVFPFCTHNCGPEYRHFTQKWYADLQNAASRPATENTEN"
     gene            complement(5360..6655)
                     /locus_tag="PLK08_04170"
     CDS             complement(5360..6655)
                     /locus_tag="PLK08_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52730.1"
                     /translation="MAELEKMNRLAGRTLWVPRMSYSAARLFSAVFRSVGVNARECED
                     SDERTLELGALHTSGEECYPAKITMGDFLRIVDSPDFDSSKHAFFMPTANGPCRFGQY
                     SPYLQKLLRDMGYGDVPVISPTSENGYDGMTDGTKSRVEMTRSLWRGIVCGDLLLKML
                     LKTRPYERTAGDADMAFEAALARVEKVLETPGLSAGKRLQKLATAMSLSRDDFRRVPA
                     KYTKDRPLIGVVGEIFCRMNTFSNYDACRQIEAHGGECWLSDICEWVWYTNWSQKEKL
                     KRDRKVFSLESLAAVVRNKVQRSDESALAASFADDFVGYEEPHDIYEDVLKKSWPYLP
                     ADSALGEMTLSAGKSIYLYEKGADGIIDISPFGCMNGIVTEAVYHDISADCDEIPIRN
                     FYFDATTSNLQRDLDIFMALARSYRTRKTQKRVYPNFFE"
BASE COUNT         1591 a         1890 c         1645 g         1600 t
ORIGIN      
        1 atcggaatca gccatgctct tggctgccgt agctgcctgc gaaatcaccg acccgccgcc
       61 gttttgagac atctccggca gtgaaccggc agacaaattc ccgcttttgg tattgtcgtt
      121 cagattgacg tctacctgct tgggcgcatt gacctgaacg cagccggcaa caattaccgc
      181 caccaaaagc accgccgaaa cacgagagac aaaacgcata aatatttctc cctgttttta
      241 aacttcagca cgcccggagt ttttaaaatt gaactgcttc agaacagttc tctaatcctc
      301 tttgtccgaa gacgtaaagc tcttgccgta actcttccaa tattccttgt cagtgctgta
      361 tttgacggtc tctttctttt cgccgcctac gttcacgttg acttctttgg gggcgttgag
      421 ctgaaaacac cctatcgcaa aacatcccaa taccacgcta atcaatatga atcgcatgtt
      481 ttcactccct gaactttacc gtcagttttc aatagttgaa atgccgtcgg gtattatggc
      541 attttcgcga atcacaagtt tatacccgca ggacgtacag cttttcccgc tgccgttaac
      601 gaccagccgt tcaagtccaa gttcaggcgg cagcttggct gaatcgagcg taaacactct
      661 gccgtcatca agaatcacgc tgaatctgaa ttccttgccg tcacggctga aaaccgtgtt
      721 ttcattcgtc agataaaaca gccggaaatt ttttgcccag agttcacgcc atttttcagt
      781 atcatctttg tcgcaaagtt cacgcagcaa ttccctgtga gccgacgatt tcagaaatcg
      841 ttcatccatg aaatatatgt atggcgacag cgtaaaaact ctgtctttcg ttgcatccgc
      901 gttgtcgttc atgcagatga aatccgttcc tttttcgccc gacggattca tcgccgcaaa
      961 gatgttcctg ttcagagacg tcatgtcgcc tatgccaatc cacactataa tgaacgccag
     1021 caactgccac aaaaatgaaa ttgccctgcc gcaatctatt ttgcctatcc acatttttct
     1081 gcctgcagac catcggagaa aaataactag caataacatg agcggcagcg tcgcgacaga
     1141 cagcatggtt gcagcgttaa acggattatc cggctgaacc ggcagaagcg agccgtaaac
     1201 ggcgatgttt ttcaacagcc acaaaatcag catcgctcca accggcgaaa acagccagcc
     1261 catccgccgc atgaccgtcg gctgaagatt tgcttcatca gcattatttg cctgattttg
     1321 acgctgcctg gaaaaaaggc tgaacgcaaa cgccgccgtc gccggcagaa ttacgaccgc
     1381 ctgcatatat tgcatcataa tcgcccacgc aatcatcaat cccgcaagaa tatgaacgcc
     1441 ccgccgcgag ccgtctgcga cgctgagcgt caccaacgcc acaatgacca gtccgcccga
     1501 cgcatatgcg tcaaaatttc cacagcggct gaccgccacg ccccaggcgg tcaacaccag
     1561 caccagcagc cacctgtcga gataattcag tccggcccgc cggccgatac cggacgcgag
     1621 catcatgccg gccaacagat aaataaaccc aacaactcca gccgccatcg caccgccaaa
     1681 gagcatcagc gccgcgatat tcatttcagc cagcatgccg cccgcggcaa aaagttccac
     1741 gccgccagcc gcaccgcccg ttatcccgcc gtaacatgcc gccgttttcg caaccagaaa
     1801 tttgccggcg gtctcataat tatcaggcgg gaaaaacgac gcaacaagca tcgcagccag
     1861 cccggccaat atcagccagt gaaggatttt taccgaaata tcactttccc gatacggatg
     1921 cgtcacgatg atttttatgt gtcgccatgc gagattgaca tctttcgcag cacttctgcc
     1981 gccgagcatc acggctacga tgcaggctaa cattactatc agccggccca gccatccgcc
     2041 gcctggcaat atcgccaata tcagccatgc cgtacccaaa atgcccgtgc cgcacagaaa
     2101 ttccgcacaa agcgtttcca cgcgatcgaa gttttttttc aaaaattcat cgccggtcag
     2161 ccgacgaaaa accgccgcac cggctgccgt gcagacgaag catccgactg acaatatcag
     2221 gatgttccag atggcataaa acatgttgta ctcagggtag caatccgacg ttcgcaagta
     2281 aagttttttt gaggcaaact gccgctttca gccgaggaat ccagccagcc gcgtaagtac
     2341 ccggccgacg acaatttccg gacttgacgc cccagcgagt atgcctattt tcaacggccg
     2401 catagccggc agccagtttt ttatcactgc cggctcagat gtctcggcgt tccaggctaa
     2461 cagttcgttt tcgttatgaa tcgcgctgtt atcttctata aggtatgaag gggcaaactg
     2521 cctggccagt tcgtaaagat ggcgggtatt cgacgatgta aaaccgccca cgactatcac
     2581 aatgtcgcag ccgcttttac ataattcaac cgacgcattc tgccgcgcct gtgtagcctt
     2641 acagactgtg ggctgaaagg ccaggtcctc gcggctggcg catggcctta cgtcaaaggc
     2701 ggccttgagc atggcctgaa ccttcatcgt atcgttatat agcatggtag tctgcgaaac
     2761 ctgggccagc cgcatgaaac tgtcgatatc gccggcgttg gtggcatggg gcggaaatac
     2821 agaaaacgct tcatcggatt tgccggcgaa catgtcgcag aataatcgta cctggttgag
     2881 attttcgacg acgagatatt tgccgccggc ggcttcgagc cggcttttcg tgacgacggt
     2941 ttcgtcgtgg agagaacggc cgaagatgag gattgaatag ccgccggcga cggctttctc
     3001 cgaccagcgc cacagccgta tgacgtcgcc gcaggaacag tcgattatcc ggcagccgat
     3061 ttcacgaagc cgtttctcta tcggcaacgc aacgccgaac gctggtatga ttacggtatc
     3121 gccggatttg acatgcttct ctatccggtc gcagcgacgc tcttccgccg aaagtatttt
     3181 tacgccgtgt tcgctaaagt aatcgttaac ccagggattg tgtataatct cgccgagcag
     3241 aatcaggcgg gacgtatcat tattgcgacg gctattttct accgcctcga cagccatcat
     3301 caatgcgcgt ttgactccgc ggcagaatcc gaaaacttcc ggcagacgca gttcgccgcc
     3361 aggcagtttc cacacgttcg gcgtatgggc tgagcggatg tcgtctatta tcgtcgaatg
     3421 gaaggcttcg agtgggtctt tcatatcagt tttccgtgtt ctcagtggct ggacggctag
     3481 ccgcattctg caaatcagca taccattttt gagtgaaatg acggtattcc ggcccgcaat
     3541 tatgcgtgca gaacggaaaa acgccagccg gcgtggcata gagaataacg catcgcttta
     3601 cgcgctccac gtcgaaattg tacctgtcct gaaaatgcat gccggcggaa agcagcgtgc
     3661 gataattttt ctcgctgccc ttgcccctgc cgatacgttt atcgaccatg cccataatcg
     3721 accgcatcag gagcgaaatt tttatgtctt taggcgcagt cttccaccgc cagtttttca
     3781 taaacagcca aacaatcctc agcttgtcaa aaaagttcgc cgttctgcct gccgccttga
     3841 ttttgttcgc caatacgttc atgccggcga aaaggccttc cacgtccaat accgccggaa
     3901 acggccaagc ctggccagac ggcgagacga tgaaatacgt ccccatagca caatcaggat
     3961 ggcagctcgg ccgcactttg ggattgtccg taatcgcttc gagcatgttc gaaagcggca
     4021 cgactatgct cagcggcatg atgtcacgca tcggcacgac gttggaacat ttggccatat
     4081 cgtgcgccag gtcgccaagc gtataacgat gcgtttgcag ctcttcgacg ctcatgcggc
     4141 cggtaaaact caccggctgc cagcttatgc cggaaatcac gtccacattc ctgaacgcga
     4201 agtcgaggat tttcggcacc tgctcatcat tgatgttttt gacgattgtc ggcacaagac
     4261 acactttcat gccgaccttg cgggcgttct ctatggcggc gagttttttt gaccatatgc
     4321 ccggataatt tcgcgtctcc ttatatgctt cgtcgccgac gccgtcgaat tgcagataca
     4381 gcgtgtgaag gccggcatcg cggcattgac gggcaaaatt ctcgtccgac aaggttatgc
     4441 cgttggtggc tatctggatg tagctaaaac ccatatcgcg cgcggcggag atgattttca
     4501 gaaaatccgg atgaatcgtc ggctcgccgc cggtaaattg tatagcagtc gtcggatgcg
     4561 gtttcatcgc gcgaagcact tttaactcgc gcacgatttc attgtagtcc ggctcccata
     4621 tataaccctg cgcgttggcg ttggcaaaac agatgggaca tttcatattg cagcggttag
     4681 tgaggtctat ctgcgccagg cacggcccgg aaagatgctg attgcaaagg ccgcaatcgc
     4741 tcggacaatt tttgccgtcg gtaacatgcg gaaactccaa tcccgcgtgt tcttcgaaac
     4801 tccaccacga cgcttttgca aaaagtttga cgtccgaatt tatcctgtcg cggaaatacc
     4861 cgtggtcggg gcatgtcttt tctatccaga cggctgagtc ctgcacaaaa tatctgcctg
     4921 gaataatcgc gccgcattcg gggcagagag tttcaacggt gcgcggcaga cgtcgcgaga
     4981 ctggacggat acggcagccg gaaaaagttt cagtcgcact gcccggcagg tcacttgcgc
     5041 gattcgtcca gattgcgtca tcgtgaacaa tgggcggctg ctcgccgcag tgaggacagt
     5101 tgaacgccag cacaacctgc gatttatgcc tccggaattc agcaggcact acacgatgac
     5161 acttcggaca ggtagcccgc gttgaaacag gcaactcgcg atatgacccg acgaaacgcc
     5221 gcatcgtatc acgaaattct ccgagttctt taatttcagt ttgcattttc gctcattaag
     5281 ttttttattt attccaagtg accgattgac tgtattttgg ttcggtaaaa cgttaaatcg
     5341 gtcagtttga attattcatt cattcaaaaa aattcggata aacgcgcttt tgggtttttc
     5401 gcgtgcggta actgcgggcg agagccatga atatgtcaag gtcgcgctgc aggttcgacg
     5461 tagtggcgtc gaagtaaaaa ttgcgtatcg ggatttcgtc gcaatccgcg ctgatgtcgt
     5521 gatagacggc ttccgtgacg attccgttca tgcagccgaa ggggctgatg tctattatgc
     5581 cgtcagcccc tttttcatat agatatatgc ttttgccggc ggagagcgtc atttcgccca
     5641 gcgcactgtc agccggcaga tacggccacg attttttcag cacatcttca taaatatcat
     5701 gcggctcttc atagccgacg aaatcgtctg caaacgaagc ggcaagggcg ctttcatcgc
     5761 tgcgctgcac cttgttccgg acgacggcgg ctaacgattc gagtgaaaaa acttttctgt
     5821 cgcgtttgag tttttctttc tgcgaccagt tggtatacca gacccactcg cagatgtcgc
     5881 tcagccagca ttcgccgccg tgcgcctcta tctgacgaca ggcgtcgtaa ttgctgaacg
     5941 tgttcatgcg gcagaaaatt tcgccgacta cgccgataag cggccggtct ttggtgtatt
     6001 tcgccggcac acgtcgaaaa tcgtcgcggg agagcgacat ggcggtcgcg agtttttgca
     6061 gccgttttcc ggcagacagg ccaggcgttt ccagaacttt ttccaccctt gccagggcag
     6121 cctcgaaagc catgtcggcg tcgccagcgg tgcgctcgta aggccgcgtt ttgagcagca
     6181 ttttcaagag caggtcgccg cagactatcc cacgccacaa agaccgcgtc atttcgacac
     6241 gcgatttcgt gccgtcagtc atgccgtcgt aaccgttctc gctcgtgggt gaaatgaccg
     6301 gcacatcgcc atatcccatg tcgcgcaaca gcttttgcag atagggagaa tactggccga
     6361 atctgcaagg gccgttggcg gtcggcatga aaaaggcgtg cttagacgaa tcgaaatccg
     6421 gcgaatcgac gattcgcaaa aaatctccca tcgttatttt ggcgggataa cattcttcgc
     6481 cggacgtatg cagcgcaccc agctcaagcg tacgttcgtc tgaatcttcg cactcgcggg
     6541 catttacgcc cacggaacga aagacggctg aaaacagcct ggctgccgag taactcatcc
     6601 gaggcaccca cagcgttctg ccggcgagac gattcatttt ttcaagttca gccactttca
     6661 gttccaaatt tttaaaaaag tgaatctcta cgggttaaaa cccgtagcat catattcatc
     6721 aggctt
//
LOCUS       DAONFI010000091         6724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13281_length_6724_cov_4.265857, whole genome shotgun sequence.
ACCESSION   DAONFI010000091 DAONFI010000000
VERSION     DAONFI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13281_length_6724_cov_4.265857"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLK08_04175"
     CDS             complement(<1..895)
                     /locus_tag="PLK08_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52731.1"
                     /translation="MNGRICVYIAICFVAIFAAAICIAGDGDRPAGKTVTVTVQNTDA
                     PRSMPVTAARPSDARFKIVQVYVALCDNRYQGIEKVPASLGDGQKPSTNLYWGAMYGV
                     KTFFRKSYNWKVWPYFNAANKKRGILDVAIFESQGLGPKVYVVAFAYAGNRMRDTLRD
                     FFAAAAGEKKAIIETHVNGKKFQLPIEGFADLVCFVGHNGLMDVKPGTLGSFRPAAVH
                     PGNAVVLACKSQVNFSPILEKLNCNPLITTTTFMAPEAYTLDAIVREWAFGGTAEMIR
                     REAAKVYAKYQNCHVSAAMQIF"
     gene            1055..2596
                     /gene="metG"
                     /locus_tag="PLK08_04180"
     CDS             1055..2596
                     /gene="metG"
                     /locus_tag="PLK08_04180"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPS52732.1"
                     /translation="MSNTFYVTTPIYYVNDAPHIGHSYTTIAADVLARYNRTAGRDVY
                     FLTGTDEHGIKMVKAASEKTLSPKELADINAPHFKSLWEELNITNSDFIRTTQPRHIK
                     RVQEMVRRLVDRGEIFLGKYEGWYDEGQEEYVTETTAKENDYKSAINGKPLVKYSEEN
                     WFFRLTKWTPKLIAHIEANPDFIQPAARRNEVLSKLRIGVDDLCVSRRKEKLPWGIEM
                     PNDNNHVVYVWIDALSNYINALGLIEVGDEYDGKFAKYWPASVHLIGKDILWFHAVYW
                     PCILMALDIPLPKTVFAHGWWTSNGRKMSKSLGNFVNRETIEKYCLDYHRDVYRYYLL
                     RAVTFGGDGDFSADMFKQIYNTELANGVGNLLSRTIKMISKYFDGRIPAAGPAVEQAA
                     AVEKAATELTQIADELMAHCAFSKYTDNVMALVTATNTFIDQTEPFRLAKDESQKPRL
                     ATIMNTCAQAVRIVLQYLAPFMPEKTAQGLATLGCRTGELGKFGELPAGTAVNPGEPL
                     FMRKQ"
     gene            2753..3142
                     /locus_tag="PLK08_04185"
     CDS             2753..3142
                     /locus_tag="PLK08_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52733.1"
                     /translation="MKKIPRSEIERIIKKDGRYHPDAFRFLHEALSRAVNEVHGENAS
                     PGGHVTGQQLCMSIRDLAIDRWGMMAKAVFNRWGIRESIDFGNMVYLMIEHGIFRKND
                     NDSVEDFRNVFNLDRDFDISGRIRLKQ"
     gene            3142..3318
                     /locus_tag="PLK08_04190"
     CDS             3142..3318
                     /locus_tag="PLK08_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52734.1"
                     /translation="MKKKTQERMLTAPIDVSDGIPDRVKQPVAKRFILVLTVYVLWLV
                     VLMYFVIAGGGTKF"
     gene            3392..4408
                     /locus_tag="PLK08_04195"
     CDS             3392..4408
                     /locus_tag="PLK08_04195"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPS52735.1"
                     /translation="MNLARNLSVILIMAAAFAIVGCQQPAVQSEWNISPDKLCRVSEI
                     ADRLGMRLDKSTSAVARMSSPHNSVIIYGDPDGRVLVNNTEVTRSINILPDGDELLVP
                     YKVEREIRAKLRFTGEPAAAAAKRDARIAAAHHKTYTQPTYANPPKPKWMSGSVVVVD
                     AGHGGKDPGALGGNNTREKDVVLDIALVVAAKLRDKGHDVKLTRTGDYFIPLDGRVDM
                     AEKAHPDLFVSIHADAAGSSAATGMTVYIPKRGDDYNMSLRAGKLVESQAGTVVSNSR
                     GVQKHSKNLRVLERTSCPAMLIEVGFLSNPAEEQQLASSEYRRRVADSIANGIDKYLR
                     STGK"
     gene            4487..6676
                     /locus_tag="PLK08_04200"
     CDS             4487..6676
                     /locus_tag="PLK08_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPS52736.1"
                     /translation="MIIKGSLSLSQQESGRLNYNRFNFLNGLSYICLGDTIIILFAIK
                     LGCPDYCISILGSLIFLSNFCMPLGKFLMARVGAIRTIASCWTMRNISILLVASAPFF
                     LWLAGLKAAMVTIILGAFAFYACRSIGVIGFQPVMGEITTDENRGKFTSVNSMLFNIA
                     NLIMLGAIIMLMMFSHDIWVFIVTILTGAVLGIASTWFISRINETSAIKASAAEPILS
                     DMILTLKNPLRRRQLFACCAISSAITLTVPISMLALKKGYGISDDSALIFALIQMAGA
                     IAIAYVVGLLAEETGPRPLAIIAYCMLIVLCILWIICPTTFKWYYAAVLFMLAGAGVT
                     GTGVTMAHYFLIVIPHRERVASTLTINVISGIAAGLAGSVIGAGILQYLDVKGITGME
                     TFKIYFASVLALLLAGLFFVFRLAPLAEWKLRDVLGLIFSPRDLNTLRTLYKIKKIDA
                     PDTECEDIDRLNEVKSGLSENALLSYLDSPKFSVRANSLTALGEIPFGEKTVKALLKE
                     LEKGEYTTAHIAAQIAGEHKIRSAIPLLREYLDSGDLYLQGKTFIALSQLHDAESYGR
                     IKTTFRNTDNPRLIIQGATALTNIADDESFRIMLEKTTHEMPRQVLFEVICSIMCLAG
                     CGDEAYKFLRLYIRNQTSTLALLYLGELSQPDIQKRDADKKIIFDFDSNTISNAGMVN
                     ILLNRIGRTAEPESKASLVAEFLRSAQPDSLPKELMLCLIPFTARAY"
BASE COUNT         1759 a         1833 c         1660 g         1472 t
ORIGIN      
        1 agaatatttg catcgcagca gagacgtgac agttctgata ttttgcataa accttagcgg
       61 cttcgcgacg tatcatctcg gcagtccccc cgaaagccca ttcgcggaca atcgcatcca
      121 gcgtatacgc ctcaggagcc atgaacgtcg tcgtggttat cagcggattg caattcagct
      181 tttccaatat cggcgaaaag ttcacctgcg atttgcatgc cagcacgacg gcattgccgg
      241 gatgaaccgc cgccggccgg aaagaaccca acgtgccggg ttttacgtcc atcagtccgt
      301 tatggccgac gaagcatacc aggtcggcaa agccttctat gggaagctga aattttttgc
      361 cgttgacatg ggtttcgatg attgcttttt tttcgccggc tgctgccgcg aagaaatccc
      421 tcagcgtatc tctcatgcga ttgcctgcgt atgcgaacgc aacgacgtag actttaggac
      481 caaggccctg actctcaaaa atggccacat ccagtatgcc gcgtttttta ttagccgcgt
      541 taaaatacgg ccagactttc cagttgtacg acttgcggaa aaacgtcttt acgccgtaca
      601 tggcgcccca gtacaggttg gtggaaggct tttggccgtc gcccagcgat gcgggcactt
      661 tttcaatccc ctgatatctg ttgtcgcata gcgccacata gacctgcaca atcttgaatc
      721 tggcgtcgga cggcctggcg gctgtaacag gcatcgagcg tggggcatca gtgttctgta
      781 ccgtcacggt aaccgtcttg ccggctggcc tgtcgccgtc gccggcaatg catatcgcgg
      841 ctgcgaatat cgcaacaaag catatcgcta tgtacacgca aatacgtccg ttcatccgtg
      901 acctcactat acaagacaag agtttaaatt cagccggcct gccatgcaag taaaatacga
      961 cacagccgcc gtcggctgtt gaatttccat aaaaaatctg ggataatgac gacttacccg
     1021 acttcggtaa ttttatatta ttggaactgc aatcatgagc aatacttttt acgttaccac
     1081 accgatatat tacgtcaacg atgcgccgca tatcggccat tcatatacga ctattgcggc
     1141 tgacgtgctg gccaggtaca accgtaccgc cggacgcgac gtatacttcc tgacgggcac
     1201 ggacgaacac ggcatcaaaa tggtcaaagc cgccagtgaa aaaacactct ccccaaagga
     1261 actcgccgac ataaacgccc cgcatttcaa aagtctctgg gaagaactca atatcaccaa
     1321 cagcgatttc atccgcacta cacagccgcg gcacatcaag cgcgtacagg agatggttcg
     1381 ccggctggtc gaccgcggag aaatcttttt gggcaaatac gaaggctggt acgacgaagg
     1441 ccaggaagaa tacgtcaccg aaaccaccgc taaagaaaac gattacaaaa gcgccatcaa
     1501 cggcaaaccg ctggtcaaat acagcgagga aaactggttt ttccgcctga cgaaatggac
     1561 gcctaaactc atcgcccaca tcgaggcaaa ccccgacttc attcagccgg ctgcccgccg
     1621 caatgaagtc ctcagcaaac tcaggatagg cgtagacgac ctctgcgtat cccgccgcaa
     1681 ggagaaactc ccctggggca tagaaatgcc caacgacaac aaccacgtgg tatacgtatg
     1741 gattgacgcg ctgagcaatt acataaacgc gctggggctg atagaggtag gcgacgaata
     1801 cgacggcaaa ttcgccaaat actggccggc gtcggtgcat ctaatcggca aggatatttt
     1861 gtggtttcac gcagtatact ggccgtgcat actgatggcg ttggacatac cgctgccgaa
     1921 aaccgtcttc gcccacggct ggtggacgag caatggcaga aaaatgtcca aatcactcgg
     1981 aaatttcgtc aaccgcgaaa ccattgaaaa atactgtctg gactatcatc gcgacgtgta
     2041 tcgctattac ctgcttcgcg cggtgacgtt cggcggcgac ggcgattttt cagccgacat
     2101 gtttaagcag atttacaaca cggaactggc taacggcgta ggcaacctgt taagtcgcac
     2161 catcaaaatg atatcgaaat attttgacgg cagaattcca gccgccggcc cggccgtaga
     2221 acaggccgcc gcagtggaaa aggctgcaac cgaactgacg caaatagccg atgaactcat
     2281 ggctcactgc gcgttcagca aatataccga taacgtcatg gccctcgtga cggcgacaaa
     2341 tacgttcata gaccagaccg agccgttcag gctggctaaa gacgaatcgc aaaaacccag
     2401 gctagccacg attatgaaca cctgtgcaca ggcggtacga atagtgttgc agtacctcgc
     2461 tccgttcatg ccggaaaaaa ccgcgcaggg ccttgccacg ctgggctgcc ggacaggtga
     2521 actcggcaaa ttcggcgaac tgccagccgg aactgccgta aaccctggcg aaccgctgtt
     2581 catgagaaaa cagtaaatgg aaaatcaacc agcgtccgca tttttcggca cacctgtcga
     2641 atttgcttga atcctgccga ctgtttgatg aaactttgaa tattgtcgtt atctgctgtt
     2701 tgttttttgg gatttttcgg cgaaaatatc tcatgcaaga ggcaacggac ttatgaaaaa
     2761 gatacctcgt tcagagatag agcggataat aaagaaagac ggacgttatc atcccgacgc
     2821 attccgtttt cttcatgagg cgttgagcag agccgtcaac gaagttcacg gcgaaaacgc
     2881 ctctcctggc ggccatgtga ccggccagca gctttgcatg tccatacgcg acctggccat
     2941 cgaccgctgg ggcatgatgg ccaaggcggt cttcaaccga tggggcatcc gcgaaagcat
     3001 agatttcggc aatatggttt atctgatgat agaacacgga attttccgaa aaaacgataa
     3061 tgattccgtg gaggatttcc gcaacgtatt caacctggac cgcgatttcg acataagcgg
     3121 gcgaataagg cttaaacaat aatgaaaaag aagactcaag aaagaatgct tacagcgccg
     3181 atagatgttt cggacggcat acccgaccgc gtcaaacagc cagttgccaa aaggttcata
     3241 ctggtcttga ccgtctatgt actatggctt gtcgtcctga tgtatttcgt catagccggc
     3301 ggcggcacaa aattttaaac agccgacatc gacttaattt gtgcatcgcg ttttatgttt
     3361 ttgtttaagc gatgcagtat caaggtgtta gatgaatcta gccagaaatt tatccgtcat
     3421 tctgattatg gcggcggcgt ttgcaatcgt aggctgccag cagccggcag tgcagtccga
     3481 gtggaacatc tcgccggata aactctgtcg tgtaagcgaa atagccgacc gcctgggcat
     3541 gcggctggat aaatccacct ctgcggtggc tcgcatgtcc agcccgcata attccgtaat
     3601 catatacggc gaccccgacg gccgggttct ggtcaacaac acggaagtaa cccgcagcat
     3661 aaacattctg cccgacggcg atgaactgct cgtgccatac aaggtcgaaa gggaaataag
     3721 ggccaaactc cgtttcaccg gcgaaccagc tgccgctgcc gcaaaacgcg acgccagaat
     3781 cgctgccgca catcacaaaa cctatactca acccacatat gccaacccgc ctaaacccaa
     3841 atggatgagc ggttcggtgg tagtcgtcga cgccggccac ggcggcaaag accctggcgc
     3901 attgggcggc aacaacactc gtgaaaagga cgtcgtactg gatatagcac tcgtcgtagc
     3961 cgccaaactc cgcgacaaag gccatgacgt aaaactcaca cgcacgggcg attatttcat
     4021 tccgctggac ggcagagtgg atatggccga aaaagctcat cccgacctgt tcgtcagcat
     4081 acacgccgac gccgccggca gcagtgccgc aaccggcatg acggtataca tacccaaacg
     4141 cggcgacgat tacaacatgt cgctgcgagc cggcaaactc gtcgaatcgc aagccggcac
     4201 agtcgtcagc aactcacggg gagtccagaa gcacagcaaa aacctgcggg tgctcgaacg
     4261 cacatcatgc ccggctatgc tgattgaagt gggctttctg tccaacccgg ctgaagaaca
     4321 gcaactcgca agttccgaat atcgccgccg tgtagcggat tcgatagcaa acggcataga
     4381 caaatatctg cgttcaacgg gaaaatagtt ccgcttaagt ctgatttaac aacaatggct
     4441 gttttcatgc gtcgctactg ctgaaaccag ccggctgaat tacaaaatga ttatcaaggg
     4501 aagcctttcg ctttcgcagc aggaaagcgg gcggctgaat tacaaccggt tcaatttttt
     4561 aaacggccta tcctacatat gcctcgggga cactataata atcctgttcg caataaaact
     4621 gggctgtccc gattattgca tatccatcct cggctcgctg atatttctga gcaatttctg
     4681 catgccgctg ggcaagttcc tcatggccag ggtcggcgcc atcagaacca tcgcatcatg
     4741 ctggacgatg aggaacattt ccattctgct ggtcgcgtcg gccccgttct ttctgtggct
     4801 ggccggcctt aaagccgcca tggtaacaat aatcctcggc gcgttcgcat tttacgcgtg
     4861 ccgctccatc ggcgtaatcg gctttcagcc ggtaatgggc gaaataacca ccgatgaaaa
     4921 ccgcggcaaa ttcacgtccg tcaacagcat gctgttcaat atcgcgaacc tcatcatgct
     4981 cggcgcaata atcatgctga tgatgttcag ccacgatata tgggtattta tcgtcacgat
     5041 tctcaccggc gcagtactcg gcatagcgtc cacatggttc ataagccgca taaacgaaac
     5101 ttcggcgata aaagcctcgg ctgccgagcc gatactttcc gacatgattc ttacgctcaa
     5161 aaaccccctg cgcagacggc agctattcgc ctgctgcgcg ataagctccg ccataacgct
     5221 caccgtgccg atatccatgc tggcgttgaa aaaaggctac ggcatcagcg acgattccgc
     5281 ccttattttc gcccttatcc agatggccgg cgcaatcgca atcgcatacg tcgtaggcct
     5341 gctggcggaa gagaccggcc cacggccgct ggcgataatc gcctattgca tgctcatcgt
     5401 cctgtgcata ctctggataa tctgcccgac aacttttaaa tggtattatg cggcagtgct
     5461 tttcatgctc gccggcgcag gcgttaccgg cacgggtgta accatggcac attattttct
     5521 gattgtcatc ccgcacaggg aacgtgtagc ctccacgctt acaataaacg taatttccgg
     5581 catagccgcc ggcctcgcag gttctgtaat aggcgcggga attctgcaat atctcgacgt
     5641 caaaggcata accggcatgg agactttcaa aatatacttc gcaagcgtcc tggcgctttt
     5701 gctggcggga ttgtttttcg ttttccggct cgctccgctg gcggagtgga aattgaggga
     5761 tgtgctgggc ctgatattct cacccagaga cctgaacacg cttagaacgc tttataaaat
     5821 caaaaaaatc gacgcaccgg atacggaatg cgaggatata gaccgcttaa acgaagtaaa
     5881 gtcaggcctt tccgaaaacg cgctgctttc atatctcgac tccccaaaat tctccgtcag
     5941 agccaactcc cttactgccc tgggcgagat accgttcggc gaaaaaaccg tcaaagccct
     6001 gctgaaagaa ctggaaaaag gtgaatatac cactgcgcac atcgccgccc agatagccgg
     6061 cgaacataaa atcagaagcg ccattccact actgcgagaa tatctcgatt ccggcgacct
     6121 gtatcttcag ggcaaaacgt tcatcgcact gtcgcaactg cacgatgcgg agtcttatgg
     6181 caggattaaa accacttttc ggaataccga taatccgcgg ctgataatcc agggcgcgac
     6241 ggcactgacg aacatcgcag acgacgaatc attccgaatc atgctggaaa aaaccacgca
     6301 tgaaatgccc aggcaggtac tttttgaagt catatgttcg ataatgtgtc tggccggctg
     6361 cggggatgag gcatataaat ttttgcggct ttatataagg aatcagacaa gcacgctggc
     6421 gttgctgtac ctgggcgagc tgtcccaacc cgacattcaa aaacgtgatg ctgataaaaa
     6481 aatcatattc gacttcgaca gcaatacaat atccaacgcc ggcatggtca atatcctgtt
     6541 gaatcgcatc ggcagaacgg ctgagccgga atccaaagcc agtctggtgg cggaatttct
     6601 gcgcagcgcc cagcccgatt cactgccgaa agaactgatg ctctgcctca taccttttac
     6661 cgctagagca tattaagagc ttctaacaaa atgagatgtc gcaccgaggc gtagttggtt
     6721 gatt
//
LOCUS       DAONFI010000092         6700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13354_length_6700_cov_4.289842, whole genome shotgun sequence.
ACCESSION   DAONFI010000092 DAONFI010000000
VERSION     DAONFI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13354_length_6700_cov_4.289842"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..2238
                     /locus_tag="PLK08_04205"
     CDS             289..2238
                     /locus_tag="PLK08_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPS52737.1"
                     /translation="MRVGIRTKLVILLVAVALLPLLAELLTIVIGSRQMQNELLGKNV
                     QSLVSAEVNVMEVELRKNIEILSITLQEGTVLAQLEEANSTPNDPQYNDDLDRKWNIF
                     SGTSSSVRKILVNHTSQILHTIIRNNDLFVEIMVTDKYGRLIAANQKTSDYYQADEDW
                     WQKTWNGGKGEIYIPPASFDDSSKTWGVEICLPLIKGGSAIGVVKAIIDLRKWLPDCR
                     TDIGLGIGYIFLINSDGIIFHSRDIIEKMVENPQLAKYDYWKLIAVQEHPKGEWIFTP
                     NNILVSYCPLSMPKELSGIKVDMEPWTLLMEVPDNEARRSISKISLMFLLIGIVAIIG
                     IFLFGVLLIDRSIINRIRRISNAARKIAKGDLQHRVPSDWSGGRFFGKDEIDDLAHDF
                     NNMVRRLEKSYNELAEANELKENFIRIAGHELRTPVSYIVGMASLMQKSKDPERLAKA
                     IDTMGFKASRLDEIIQAMFKLIPEQSLSERVRYTSIRIEKFLADVDEAMKPWVERRHQ
                     KLIIKDETNNSIISADEGKLRDIIENLLMNAVKFTPDEGWIKLTVQRRLGGLVAFQVT
                     DQGPGIPEADQPHIFEPFFSGSDTLHHSTGKSGFGKKGMGLGLAIVKHFVDLHRGTIS
                     FISGPTGTTFTVEIPADPSWSPENH"
     gene            2274..3251
                     /gene="trpS"
                     /locus_tag="PLK08_04210"
     CDS             2274..3251
                     /gene="trpS"
                     /locus_tag="PLK08_04210"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPS52738.1"
                     /translation="MRVLSGIQPSGRLHLGNYFGAIRQFIELQRQGAECFYFLANLHS
                     LTTMRDAEKLRELSLHLAAGYLALGVDPTKSVFFLQSDVPQVNELGWFLNTVCPVSLM
                     QKAHAYKDKLAKGFSPNMALFDYPVLMAADILIYDSDLVPVGQDQKQHVEMARDLAMK
                     FNLAWNGEVLKLPDAHIVESVAIVPGLDGQKMSKSYNNTIEIFQTEKQTKKNVGQIVT
                     DSTPLEEPKNPEKCNVFALLRLFLDEDELAEVAASYRAGGYGYGHAKARLTDCINARF
                     ADARKKYDELIASPDTVRDILREGGRKARDIAEKTMQRVRAASGIITTY"
     gene            complement(3330..4220)
                     /locus_tag="PLK08_04215"
     CDS             complement(3330..4220)
                     /locus_tag="PLK08_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52739.1"
                     /translation="MKKAIFLLSLATTALLTGGCQSPDDDGKMQLSLYPATIQVTLPP
                     ANKKDIPDKPDYQFMSNVLAEIIVADGNIDTARFDIFKKDLIKVLQTLDAHGPSCNPK
                     SFETPADRMAYWYNARAFWAMYLAAKYNREMKSYYLSSLENAFYSIRFPVDGRIMTLR
                     KIDDEIFNLGGYLSLIAAPGALSGRAAIPETAFQPQNFPAIIKSRFNDFIADRRRFTI
                     DVLNMEIFVPRIIWQFHKKIMDDYQALYPTPQATFLLALSSMTSEVARFRLKNAIGYK
                     CVEAPARQTIMITGNNKQTF"
     gene            complement(4217..4981)
                     /locus_tag="PLK08_04220"
     CDS             complement(4217..4981)
                     /locus_tag="PLK08_04220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPS52740.1"
                     /translation="MSSMAEITTILFDLGETLIDFGKVNVGRMFNAGAKLAYNHLREL
                     GQTPPPRIWYFWVNYVAIRWNILKCVITQRDFHATDLMDSLCRFMGLKLSKDDLLRLC
                     WFWYEPLHNFGKVEAGLPEMLRDFTHAGLKLGVVSNTFIPGPILDRHLEEVGLLEYLP
                     FRVYSCDVGWQKPKPIIFRRALELTGSQADKTIFVGDREYNDIYGAKRVGMKTVRKNP
                     DGCRRSAADFKIRKLLELREILREFTTPQPLEETGI"
     gene            5138..6148
                     /locus_tag="PLK08_04225"
     CDS             5138..6148
                     /locus_tag="PLK08_04225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPS52741.1"
                     /translation="MNLITGATGLLGSHIAEKLVEMGQPVRALVRKNSDTRILEAWGV
                     ELVYGDVTEPKSIAEAMKGVKTVYHSAAGVGDWGSWANYFVPVTIGGTRNMLEAAKRE
                     NVERFLHISSISSYGHIMTDDIVDETWPLGELGKNLNKWSYYSRAKIEAEKLVRQAHA
                     AGDICATIAKPSWLYGPRDRASMPRIIKAIRARKTKLLGRGDNKLNLTYAGNEAEGCI
                     LAATMECAKGQTYNLSSDGDITQAEYFNKIAECLGVKPITRKVPFKVAYNAAFLMELF
                     GHITNRKTPPLVTRYSVWLIGRKCFFSPEKARRELGWKPTVGYEEGIQRSVRWCIDNL
                     GL"
     gene            6300..>6700
                     /locus_tag="PLK08_04230"
     CDS             6300..>6700
                     /locus_tag="PLK08_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPS52742.1"
                     /translation="MIARISGRVEEVTSASVLIDSGGGLCYEVLVAACDVGRLASRLG
                     QEVVLHTIHIIDGDPTRGSLTPRLIGFLSQSDRAFFREFTKVKGIGVRKALRALVRPV
                     AEIAAAIENKDNNALVALPEIGKRMAEQIIA"
BASE COUNT         1703 a         1639 c         1766 g         1592 t
ORIGIN      
        1 ccgccggcgt actcgtcggg gatgcttatg aataagccgc aaatcttgca ataacagtca
       61 atcatccgct atgaaactcc tgctcggtta tgccgccatt ttacatgtcg ccagccgaag
      121 cgtcaaaagc atttccgcaa aagtcgtcgg ctgatgaaga taaacaaaac cagcagacgg
      181 ctaaaaaaca ttggacacgg aactgcagat aacctattct actatggaat ataaagtaag
      241 gtcgcataaa agtatgacga gtggaaaaat ggcgttgtgt ggtcacggat gagagtaggc
      301 atacgcacaa aactggttat tcttctggtg gcggtggcac tgctgccgct cctggcggaa
      361 ctgttgacga tagttatcgg cagcaggcag atgcaaaacg aacttctcgg aaaaaacgtt
      421 cagtctcttg tctccgcgga agtaaacgtc atggaggtcg aacttcgcaa aaatatcgaa
      481 attctcagca taacgcttca ggaaggcact gtgctggctc agctggagga agccaactcc
      541 accccgaacg acccgcaata taatgacgac ctggacagga aatggaatat cttttccggc
      601 acgtcgagtt ccgtccgtaa aattctggta aaccacactt ctcagatact ccataccata
      661 ataaggaata acgacctttt tgttgaaatt atggttacgg acaaatatgg ccggctgata
      721 gctgccaatc agaagacttc ggattactat caggccgacg aggactggtg gcagaaaaca
      781 tggaacggcg gcaaagggga aatatatata ccgccggcga gttttgacga ctcttccaag
      841 acatggggag tggaaatatg tctgccgctc atcaagggcg gctcggcgat aggcgtggtc
      901 aaggcgatta tagaccttcg caaatggctg ccggactgcc gcacggacat aggcctcggc
      961 atagggtata ttttcctcat caatagcgac ggcatcatat tccatagccg cgatataatc
     1021 gaaaaaatgg tcgaaaatcc gcaactcgcc aaatatgatt actggaagct catcgcggtg
     1081 caggagcatc ccaaaggcga gtggattttt acgcctaaca atattcttgt gtcatattgc
     1141 ccgctatcga tgccgaagga actcagcgga atcaaggtcg atatggagcc ctggacgctg
     1201 cttatggaag tccccgacaa tgaagccaga cgcagcatat cgaaaatcag cctgatgttt
     1261 ctgcttatcg ggatagtcgc cataatcggc atattcctgt tcggcgtact tcttatcgac
     1321 aggagcataa taaaccgcat caggcgcata agcaacgccg ccaggaaaat cgccaagggc
     1381 gaccttcagc accgtgtgcc gagcgactgg tccggcggac gattcttcgg caaggatgag
     1441 atagacgatt tggctcacga cttcaataac atggtcaggc gtctagagaa atcgtataat
     1501 gagttggcgg aggctaatga actcaaggag aactttatac gcatcgccgg ccatgaactt
     1561 cgtacgccgg tgagttacat cgtcggcatg gccagcctca tgcagaagtc caaagatccg
     1621 gagcgacttg cgaaggctat agatacgatg ggcttcaaag ctagccggct ggacgaaatc
     1681 attcaggcaa tgttcaagct gattcctgaa cagtcgcttt cggaacgggt acgttacacg
     1741 agtatccgca tagaaaagtt tttggcagat gtcgacgaag ccatgaagcc ctgggtcgaa
     1801 cgccgtcatc agaaactcat catcaaggac gagaccaaca attccataat atccgccgac
     1861 gaaggcaaat tgcgggatat tatcgaaaat ttactgatga acgcggtgaa atttacgccg
     1921 gatgaaggct ggataaagtt gaccgtacag cggcggcttg gcgggctggt ggcgtttcag
     1981 gttaccgacc aggggccggg cattcctgag gctgaccagc cgcatatttt tgagccgttc
     2041 ttcagcgggt cagatacgct gcatcattcg acgggcaaga gcgggttcgg caaaaagggc
     2101 atgggcctgg gcctggcgat tgtgaaacat tttgtcgatt tgcatcgcgg tacgatttca
     2161 ttcatttccg gcccgacggg cacgactttt accgttgaaa ttccagccga cccgtcttgg
     2221 tcgcctgaaa atcattgaca tggcattgtt cgacaactac attctgtgac attatgagag
     2281 tattatcagg catacaaccg tcggggcggc tgcaccttgg gaattatttt ggtgcaatcc
     2341 gccagtttat agaacttcaa cgccagggtg cagaatgctt ttattttctg gctaatttgc
     2401 actcactgac gaccatgcgc gatgcggaaa aacttcgcga actttcgttg cacttagccg
     2461 ccggttatct ggctcttggg gtagatccga caaaaagcgt cttcttcctg cagtcggatg
     2521 tgccgcaggt aaatgaactc ggctggtttc ttaataccgt ctgtccggtt tcgctcatgc
     2581 agaaagccca tgcctacaag gataagctgg ccaaaggttt ttcgccgaat atggcgttgt
     2641 ttgactatcc ggttctgatg gcggcggaca tcctcatata tgattcggat ttggtgccgg
     2701 tcggccagga ccagaaacag cacgttgaga tggcacgcga ccttgcgatg aaattcaatc
     2761 tcgcctggaa tggcgaagtg ctcaagttgc ccgacgcaca tatagtggag tcggtcgcca
     2821 ttgtgccagg ccttgatggc cagaagatgt ccaagagtta taacaatacc atagaaattt
     2881 ttcagaccga aaagcagacc aaaaaaaacg tcggccagat tgtgaccgat tctacgccgc
     2941 tggaagagcc caaaaatccg gaaaagtgta acgtttttgc actgctgcgg ctgtttttgg
     3001 acgaagatga gctggcggag gtggctgcgt cttatcgagc cggcggttac ggctatggcc
     3061 acgccaaggc cagacttacc gactgcatca acgccagatt tgccgatgcc cgcaaaaaat
     3121 acgacgaact catcgccagc cctgataccg tcagggatat tttgcgggaa ggcggcagaa
     3181 aggctcgcga tattgcggaa aaaaccatgc agcgagtccg cgccgccagc ggcattatta
     3241 cgacgtatta agagcttata acaaaatgaa acatagtacc tggtacggtt agctgaatgg
     3301 ctggccagga aggcaaaacc ggttctagtt taaaaagttt gtttattgtt gccggttatc
     3361 attatcgtct gtctggcagg ggcttcaacg catttgtaac cgatggcgtt tttcagcctg
     3421 aaacgcgcaa cctcgctggt cattgatgac aacgccagta aaaacgtcgc ctgcggcgta
     3481 ggataaagtg cctgataatc gtccattatt tttttgtgga actgccatat tatccgaggc
     3541 acgaatatct ccatattaag cacatcgatg gtgaaacggc gacggtcggc gatgaagtcg
     3601 ttaaaacgcg acttgattat agccggaaaa ttttggggct gaaacgctgt ttcgggtatt
     3661 gccgctctgc cgctgagtgc cccgggtgcg gctatgagcg acagataacc gcccagattg
     3721 aatatttcat cgtcgatttt tctcagggtc attatccggc cgtctacggg aaatctgatt
     3781 gagtaaaatg cattttcaag tgaactcaga taatagctct tcatttccct gttgtacttg
     3841 gccgccagat acattgccca gaatgccctg gcattgtacc agtatgccat tctgtcagcc
     3901 ggcgtttcaa aggattttgg attgcacgac ggcccgtggg catcgagcgt ctgaagaacc
     3961 tttatcaggt cttttttaaa gatatcgaaa cgggcggtat cgatattgcc gtcggcgacg
     4021 attatttccg ccaggacgtt gctcatgaat tgatagtcgg gcttgtcggg aatatctttt
     4081 ttattcgcag gcggcagagt tacctggatg gttgcgggat agagagaaag ttgcattttc
     4141 ccgtcgtcgt cgggagactg gcagcctcct gtcagcagtg ctgttgttgc caggctcaaa
     4201 agaaatattg cctttttcat atgcccgttt cctccaaagg ctgtggagtc gtaaattctc
     4261 tcaatatttc acgcagttcc agaagttttc tgattttgaa atcggcggcg gaacggcgac
     4321 agccgtcagg gttttttctt accgttttca tgccgaccct ctttgcgccg taaatatcat
     4381 tatattccct gtcgccgaca aagattgttt tatccgcttg cgagccggtc agttccagtg
     4441 ctcgacggaa aattatcggc ttgggtttct gccagcctac gtcgcacgaa tagaccctaa
     4501 acggcagata ctccagcagg ccgacttctt ccaggtgtct gtccagtatt gggccgggta
     4561 tgaatgtgtt tgataccacg ccgagtttaa ggccggcgtg tgtgaaatcc cgcaacattt
     4621 caggcaggcc agcctccact ttgccgaaat tgtgcagcgg ctcataccag aaccagcaca
     4681 gccgcagaag gtcgtctttg gagagtttca ggcccatgaa ccggcagagg ctgtccatga
     4741 ggtcagtggc gtggaaatcc cgctgggtta tgacgcattt gagtatgttc cagcgaattg
     4801 cgacgtaatt gacccagaaa taccaaattc gcggcggcgg cgtttggccg agttcgcgca
     4861 gatggttata cgccagcttt gcgccggcgt tgaacatgcg gcctacgttg accttgccga
     4921 aatctatcaa tgtttcgccc aggtcgaaca gtattgtagt tatttctgcc attgacgaca
     4981 ttgtacgaaa acttccgccg ttggcaaggc tgcaggataa ttcttgacgt tgtcaggggt
     5041 aatgcctata gtaaatgtcc caaaatcgtc tgctacggaa cggcaggatt aaggcggacg
     5101 gccgccttcg tgaaacggat aacataagga aagcaccatg aatcttatta ccggagcaac
     5161 cggacttctt ggcagtcata tagccgaaaa actcgttgaa atgggccagc cggttcgcgc
     5221 cctcgttcgg aaaaacagcg atacccgtat tcttgaagca tggggagttg aactggttta
     5281 tggcgatgta accgagccga agagcatcgc ggaggctatg aagggcgtaa aaaccgtcta
     5341 ccactccgct gccggcgtcg gcgactgggg cagctgggcc aactatttcg tgcccgtcac
     5401 cattggcggc acacgcaaca tgctggaagc ggcgaagcgt gaaaacgttg aacgttttct
     5461 ccacataagc tccataagct cttatggcca tataatgacg gacgatattg tcgatgaaac
     5521 ctggccgctg ggcgaacttg gcaaaaacct caacaaatgg agctattact cccgcgccaa
     5581 gatagaagcc gaaaaactcg tccggcaggc tcatgccgcc ggcgatatct gtgcgactat
     5641 cgccaagccg tcgtggctgt acggccctcg cgacagagcc tccatgcccc gaatcataaa
     5701 agccatccgt gcccgcaaaa ccaaactcct cggccgcggc gacaataaac tcaatcttac
     5761 ttacgccggc aatgaagccg aaggctgtat tctcgcagcc accatggaat gcgccaaagg
     5821 ccagacctat aacctttcca gcgacggcga tattacgcag gctgaatatt ttaacaaaat
     5881 tgccgaatgc ctcggcgtca agccgattac ccgtaaagtt ccgttcaagg tcgcctacaa
     5941 cgccgcgttc ctgatggaac tcttcggcca tattaccaat cgaaaaactc cgccgcttgt
     6001 aacgcgatac agcgtgtggc tcatcggccg aaaatgcttc ttcagcccgg aaaaggctcg
     6061 cagggaactc ggctggaaac caaccgtcgg ttacgaggaa ggcattcaac ggtcagtccg
     6121 gtggtgcatc gataatctcg gcctttaggc cgttttgttc acgcatgccg atgatagcat
     6181 atatcacgac gggtaagcat caagcgacaa ataacaagtt ttgagcttga aactttgttt
     6241 tgtagtaagg atgtaatttt cttgttgcaa gtatatttgc attctgttag gatattgcca
     6301 tgattgcaag aatttccggc agagtagagg aggtcacgtc cgcgtcggtg ttgatagatt
     6361 ccggcggcgg attgtgttac gaagtgcttg tcgccgcctg cgatgtgggt cggctggcca
     6421 gccggctcgg ccaggaagta gtgttgcata ccattcatat aatagatggc gatcctactc
     6481 gcgggtctct gacgccgcgg ctgattggat ttctttcaca gagcgacagg gcttttttca
     6541 gggaattcac gaaggtgaag ggcataggcg tcagaaaagc gcttcgcgcc ctcgtacggc
     6601 cggtggctga gatagcagcc gccatcgaaa acaaggacaa taatgcactg gtagctctgc
     6661 ctgaaatcgg caaacgcatg gctgagcaga ttatcgccga
//
LOCUS       DAONFI010000093         6672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13426_length_6672_cov_4.160949, whole genome shotgun sequence.
ACCESSION   DAONFI010000093 DAONFI010000000
VERSION     DAONFI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13426_length_6672_cov_4.160949"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1311
                     /locus_tag="PLK08_04235"
     CDS             172..1311
                     /locus_tag="PLK08_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPS52743.1"
                     /translation="MASLKKRNGTYYAQYYIAGRQKRITLQTDNYQIAKAKLSEITSA
                     LIQGTESPLPTKTPTSQALSNYIHHMQAAKTPGSVQRDVRILRMVFGPICPEIPAYKF
                     PQEHRQYISAAYFEQITTAQLTEYISANVRKFGLAPRTANRFREVLNRLFNWSMKQGG
                     IKMPCDRNPVASVERYRQKASNIRFLTLNQISEQLDALEGFPQYQAMAAVYIYAGLRR
                     EEALWLQMEDIDLSAAKYGMLRIRAKTVNGEYWQPKTAKNRAVPISSSLRAYLDNYCP
                     RPSRGGWYFPSPRGLRYNPENFSSDYAHIQKKIGLKWTCLDFRHTFGSQLAMKGESLY
                     KISTLMGNSPEICRTHYAALIPEALGDCVEFGYPKNIRQQQHSIG"
     gene            1547..4726
                     /gene="cas9"
                     /locus_tag="PLK08_04240"
     CDS             1547..4726
                     /gene="cas9"
                     /locus_tag="PLK08_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HPS52744.1"
                     /translation="MTSLICDDLVLGLDLGVNSIGWAILQNSGNTPAKILNAGVRIFD
                     AGMEGDISSGNAQSRAVNRRNARTLRRQTQRRSRRLKKIFHILQRMNLLPQSDASKII
                     PELDRELLSKYADSFKSVETVNLDNLLPYWVRKRALDEQLESYELGRALYHLAHRRGF
                     LSNRKSPQKADEELGKVKAGINELKQQITDSGSRTLGEFFAGLNPHERRIRSRWTHRD
                     MYREEFDAIWQTQQQYMPEVLNEKQYSELYNAFFHQRPLKSAKHLVGKCELEPSCRRA
                     PWATLEAQEFRMLQMLNNCQLITEDGEVKNFSDSQRTKLIEALEIQGDLKFSDAKKLL
                     GLKRTAHFTLENGGESRFVGNRVNARMLQIFSQDWQTFDDAKKEQIINDLRCYQNPAA
                     LAERAKAIYGLPAAKAEEFADIELEGSYCNISRKAIRKFLPLLREGMTFPEAKKKLYP
                     QKFQAVEPKNSIIPLAKHIDLRNPVVSRVLTELRKVVNNIVLKYGKPGIIRVELARDM
                     KNNAVKKKALTKRNRDNERVRKAAAEKIAKETIVKNPRRDDILKLQLAEECNWTCPYT
                     GRHFGMNDLFGGSPTIDIEHIIPFSRSLDDSYLNKTLCDAEYNRNVKKNMAPGEYLSP
                     DSEAWNKIIGSVSRFKGDRDTIAEKLRRFKLSAAEIEEQLVEFSSSQLNDTRYASKVA
                     ADYLGTLYGGVNDAQGKRRIQATRGGTTAFLREMWKLNNILGDGVKNRGDHRHHAIDA
                     IAVALTTPATIKTLSDAAVRECDRRTHKGRFKMPAMPWDNFWQDTKDAIDKIVVSHRV
                     KRGINGQLHDTTNYAPVKIMGEKGKPVFHVRRQLAGLKSTEVEQIVNPVIRQAVKDKL
                     DSLGISDPAKAFTDFANHPFVTAKSGKTIHIHKVRISVSASPQAIASGPRERFCNTAS
                     NHHMEVFAVLDKDGNEIRWDAVVVSMLEAKQRLKALYKQKHKDKNFKIGSIVNRNHGP
                     NTKFKFSISPGDTLQFENPSEKMPTLVHLKCVPFSQQLMFTSLNDARLQKESKAAKEW
                     FSSKVDPLRKLKPQKVMVLPLGEVIPAND"
     gene            4719..5606
                     /gene="cas1"
                     /locus_tag="PLK08_04245"
     CDS             4719..5606
                     /gene="cas1"
                     /locus_tag="PLK08_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HPS52745.1"
                     /translation="MTDRMIDISQEQASLKVRMGQLVVTTDNGETTVPIVDIGALVVS
                     HPRVTYTQAVLNELAVAGGVFIICDERYLPSAMLLPLQTHHVQTQNFAQQAAAKEPVK
                     KRVWQQIIRAKIKGQADVLKKITGDYCGLDALIPQVHSGDSTNRESVAARKYWPALFG
                     QSFRRDFDALDQNRHLNYGYAILRGIVARGICAAGLHPSIGLNHHHRNNPFCLADDLM
                     EPFRPIVDTTVCEYVRSNDFNEFGREAKINLLETLSADYNYFGENRTLFDISAHVAAS
                     LAAVFRGENKKLDLPNLGI"
     gene            5679..6023
                     /gene="cas2"
                     /locus_tag="PLK08_04250"
     CDS             5679..6023
                     /gene="cas2"
                     /locus_tag="PLK08_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPS52746.1"
                     /translation="MASHRLPLSEYRGMWLFVLFDLPVTSKQARKDYAKFRKDLIKDG
                     FSMLQFSIYARYFRNEDSSKTHRRRIEAFLPPNGQVRLLTVTDHQFARMQVFFGKIRN
                     ETEKPPQQLLLF"
     repeat_region   6073..6637
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6073..6108
                     /rpt_unit_seq="agtgtatcaaagcgacgactccctgtaaacctcagc"
BASE COUNT         1990 a         1629 c         1495 g         1558 t
ORIGIN      
        1 gtcctctcgg gcgcagtatg ataacgtctt ccaaataagg gacttgcaga aatgcgagtc
       61 cctttctttt tgtgcaaaac atggaattgt accctgaatt gtaccctacc ggataaataa
      121 aggtgggact cgcaaaaaat tataaaaata cttttttgca aggagtccat tatggcatca
      181 ttaaaaaaac gtaacggtac atattacgcc caatactaca ttgcaggccg tcagaagcgc
      241 attacacttc aaacagataa ctaccagata gcaaaggcca agctttcgga aattacatcc
      301 gcgctgattc agggcaccga aagccccttg ccgacgaaaa cccctacatc gcaggctttg
      361 tcaaactaca tccatcacat gcaagccgcc aagacccccg gcagcgtaca gagggacgtc
      421 agaatcctca ggatggtctt tggcccaata tgcccggaaa tacccgctta caaattcccg
      481 caggaacacc ggcagtacat ctccgccgca tactttgaac agataaccac cgcccagctt
      541 acggagtaca tctccgccaa tgtccgcaag tttggccttg ctccgcgaac agccaaccgc
      601 tttcgggaag tcctcaaccg gcttttcaac tggtcgatga agcagggcgg tataaaaatg
      661 ccctgcgaca ggaacccggt cgcttcagtc gagcgatatc ggcaaaaagc ctccaacata
      721 cgtttcctca cattgaacca gataagcgag cagcttgacg cattggaagg ttttccgcag
      781 tatcaggcga tggcagccgt ttacatctac gcgggccttc gacgcgagga agccctctgg
      841 ctgcaaatgg aagatattga cctatccgct gccaaatacg gcatgcttag aatccgggcc
      901 aagacggtca atggcgaata ctggcagccg aagaccgcca agaaccgagc cgtccccata
      961 tcgtcgagtt tacgggcgta tctggacaat tactgtcccc gccccagccg tgggggttgg
     1021 tatttcccca gcccgcgagg ccttcgatac aacccggaga atttttccag cgactacgcc
     1081 catattcaga aaaaaatcgg cctgaaatgg acatgccttg attttcggca tactttcggg
     1141 tcgcagcttg ccatgaaggg cgaaagtttg tataagataa gtacgttaat gggtaattcc
     1201 ccggagattt gccggacaca ttacgccgca ctgataccgg aagcccttgg cgattgtgtg
     1261 gaatttggat accccaagaa tatacgacag caacaacatt ccataggata gacacacgct
     1321 tccccgttga ctggggttaa atttaaacaa agcttgaaca ttttgtgtaa aaaagaacgc
     1381 ctctcggtaa accggggagg cgtaaagaac attctctatg gaatgactga cttgcggacg
     1441 gaaaatcctt aaccttactg aggaaagcag gataatcgtc gctttgatac actgatagta
     1501 ttctagttgt ttcggggcgg catttcaaga actaaggaga aaaaaaatga catctttgat
     1561 ttgtgatgat ttggtacttg gccttgacct tggggtaaat tcaattggtt gggccatttt
     1621 gcagaatagc ggaaatactc cagccaaaat cctcaatgct ggtgtcagaa ttttcgatgc
     1681 tggcatggaa ggcgatatat catcaggcaa tgcacaatct cgtgccgtta atcgcagaaa
     1741 tgctagaact ctccgccgac aaacacagag gcgatcaagg cgtctaaaga aaatatttca
     1801 catattacag agaatgaacc tgctacccca aagtgatgcg tcaaaaataa tcccggaatt
     1861 agacagggaa cttttgtcaa aatatgccga cagctttaag tcggtagaaa cagtgaatct
     1921 ggataatctt ttgccatatt gggtacgcaa acgggcactg gatgaacaac tagaatcgta
     1981 tgaattaggc cgtgcattat atcatttagc tcaccgacgc ggttttcttt ccaatcgaaa
     2041 atcaccgcaa aaggcagatg aagaactcgg caaagtcaaa gccggaatca atgaactcaa
     2101 acagcaaata acagattccg gcagccgcac gcttggagaa ttttttgcgg gactcaatcc
     2161 acatgaaaga cgtatccgtt cacgatggac gcaccgcgac atgtaccgtg aggaatttga
     2221 tgccatctgg caaacgcaac agcaatacat gccggaagtt cttaatgaaa agcaatattc
     2281 ggaactctat aatgcttttt tccatcaaag acctcttaag tcagcaaaac atttagtcgg
     2341 caaatgcgaa ctggaacctt catgccgtcg cgctccttgg gcaactctgg aagctcagga
     2401 atttcgcatg ttgcagatgc taaataattg ccaattaatt accgaggatg gcgaagtaaa
     2461 aaacttttcc gattctcaaa ggacaaagct catagaagcc cttgaaatac agggcgacct
     2521 gaaattcagt gacgcaaaaa aattacttgg ccttaaaaga acagctcatt ttacattgga
     2581 aaatggcggg gaaagtcgat tcgtaggcaa tagggtcaat gccagaatgc ttcagatttt
     2641 cagtcaggat tggcagacat ttgacgatgc aaaaaaagaa cagataatca atgacctgcg
     2701 gtgttatcag aaccccgccg cactcgcaga aagggcaaaa gctatatatg gcctccctgc
     2761 ggcaaaagcc gaagaattcg cagatattga gcttgaaggt agctactgta atataagtcg
     2821 caaagctatc agaaaattcc tgccgctatt gcgtgaagga atgactttcc ctgaagcaaa
     2881 gaaaaagctg tacccacaaa aattccaggc cgttgaacct aaaaattcca ttatcccgct
     2941 agccaagcat atcgacttgc gaaatccagt ggtgtcgcgc gtacttacag aactgcgaaa
     3001 ggttgtaaat aacatcgtgc tcaaatatgg caagcccggt attattcgcg tagaacttgc
     3061 gagggacatg aaaaataatg ctgttaaaaa gaaagccctt acaaagcgga atcgggacaa
     3121 tgagagagtc cgaaaagcag ccgctgaaaa aattgccaaa gaaaccattg ttaaaaatcc
     3181 ccgccgggat gatattctca aactccaact cgccgaggaa tgcaactgga cttgcccata
     3241 taccggcaga cattttggaa tgaacgacct tttcggaggc agtcctacta tcgacatcga
     3301 acacataatc cccttcagcc gcagccttga tgattcatac ctcaacaaga ctctctgcga
     3361 tgccgaatac aatcggaacg tcaagaagaa catggctccc ggtgaatacc tgtcccctga
     3421 ttcggaggca tggaacaaaa tcatcggctc ggtaagccgt ttcaaaggag acagagacac
     3481 cattgcagaa aaactccgca gattcaaact ctccgcggct gaaattgaag agcaactcgt
     3541 cgagttcagt tcaagtcagc ttaacgatac ccgctatgcc tcaaaagttg ctgccgacta
     3601 tctcggcacg ctctatggcg gtgtaaatga tgcacagggc aaacgccgta tacaggcaac
     3661 tcgcggcggt acaaccgcat ttttgcgtga aatgtggaaa ctcaacaaca tacttggtga
     3721 tggcgtaaaa aatcgtggcg accaccgtca tcacgcaata gacgcaatag ccgttgccct
     3781 taccactccc gcaacaataa aaacactttc agacgctgcc gttcgtgaat gcgaccgtcg
     3841 cacgcataaa ggtcgcttca aaatgcccgc catgccgtgg gataatttct ggcaggacac
     3901 caaagatgcc attgataaaa tcgtcgtctc gcatcgggta aaacgtggca taaacggtca
     3961 gcttcacgat acgactaatt acgccccggt aaaaattatg ggcgaaaaag gcaagcccgt
     4021 ttttcatgtc cggcgtcagc ttgccgggct aaaatcaacg gaagtcgagc agattgtcaa
     4081 ccccgtaatc cgccaagccg ttaaggacaa gctcgattcg cttggcattt cagaccctgc
     4141 gaaggccttt actgatttcg cgaatcatcc gtttgtcact gcgaagtctg gcaagaccat
     4201 tcatatccac aaagtgagaa taagcgtctc cgctagcccg caggctattg cttctgggcc
     4261 gagagaacgc ttctgcaata cggcctcaaa tcaccacatg gaagtttttg ccgttctgga
     4321 caaagacggc aatgaaatca ggtgggacgc tgtggtcgtc agtatgctcg aagcaaaaca
     4381 gcgtttaaaa gccctttaca aacagaaaca taaagacaaa aatttcaaaa tcggctctat
     4441 cgtcaatcgt aaccacgggc cgaatactaa attcaaattt tccatatctc ccggagatac
     4501 tttgcaattt gagaatcctt ctgaaaaaat gccaacatta gtgcatttaa aatgcgtgcc
     4561 gttttcacaa cagctaatgt ttacttctct taatgacgct agattgcaaa aagaaagtaa
     4621 ggccgcaaaa gaatggttca gtagcaaagt tgaccctttg agaaaactaa agccgcaaaa
     4681 ggtcatggtt ttgccgcttg gcgaggttat tcctgcaaat gactgatcga atgatagaca
     4741 tctcgcagga gcaggctagc ctgaaagttc ggatgggcca gcttgtcgtt accacggata
     4801 atggtgaaac cactgtcccg atagtggata tcggcgcatt ggttgtatca cacccccgag
     4861 taacgtatac acaagccgta ttgaatgaac ttgccgtcgc cggtggtgtt ttcataattt
     4921 gcgacgagcg gtatttgccc tcggccatgc tcctgcctct gcaaacgcac catgtgcaaa
     4981 cgcaaaattt cgcacagcag gctgccgcta aagaacctgt caaaaagcgc gtctggcagc
     5041 agattattcg cgccaaaatc aaaggccaag ccgacgtgct taaaaaaatc acaggtgatt
     5101 actgcggctt ggatgccctg ataccgcagg ttcacagtgg ggattccaca aaccgggaat
     5161 cggtagccgc cagaaaatat tggcctgcac ttttcgggca atcttttcgc agggattttg
     5221 atgcactgga ccagaacaga catttgaatt acggctatgc tattttacgc ggtatcgtag
     5281 cacgtgggat ttgcgccgcc ggcctgcatc cgtcaatagg cttgaaccat caccaccgca
     5341 ataatccttt ctgccttgcc gacgacctta tggagccatt ccggcctata gttgatacca
     5401 ccgtctgtga gtatgtccgt tcaaatgatt ttaatgaatt tggccgtgaa gcgaaaataa
     5461 atcttctcga aacgctttca gcagactata attattttgg tgagaacaga acgcttttcg
     5521 atatctcggc acatgtcgcc gcttcactgg cggctgtttt ccggggtgaa aataaaaaac
     5581 tggacttgcc gaatctggga atctgagtgt tatatttcgg attcgcggaa atggatttcc
     5641 cgacaagcta atacggatgg acttgctatg gagaaaacat ggctagtcat cgtttaccat
     5701 tgtcggagta tcgtggcatg tggttatttg ttctgttcga cctgcctgta accagtaagc
     5761 aggcccgcaa ggattatgca aaatttcgca aggatttgat aaaagacggt ttttccatgc
     5821 tgcaattttc aatctacgcc agatatttca gaaatgaaga ttccagtaaa acccaccgtc
     5881 gcagaataga ggcgttcctg ccgccaaacg gacaggtgcg cctgttgact gtaacagacc
     5941 atcagttcgc aagaatgcag gttttttttg gaaaaatccg caatgaaact gaaaaaccgc
     6001 ctcaacaact gctgcttttt tgaactgtta caggggatat cccccgcaat atcaacaagt
     6061 tacgggggat atagtgtatc aaagcgacga ctccctgtaa acctcagcgt agagcataat
     6121 aagaaaatct caccaacaag tgtatcaaag cgacgactcc ctgtaaacct cagctccttc
     6181 caccgtcaga agcttttcca agccagtgta tcaaagcgac gactccctgt aaacctcagc
     6241 acaagtttca accgtttcaa catttgttct agtgtatcaa agcgacgact ccctgtaaac
     6301 ctcagctatg aaaagttctt ggaataaact ttttgaagtg tatcaaagcg acgactccct
     6361 gtaaacctca gcttgaaaat ccgaggttaa gcataaatat atagtgtatc aaagcgacga
     6421 ctccctgtaa acctcagcct gacgcgacgg tcggcgaatt gataccggag tgtatcaaag
     6481 cgacgactcc ctgtaaacct cagccaaaga ctgattggca aatcatcgaa aacccagtgt
     6541 atcaaagcga cgactccctg taaacctcag cccaccaatt aaactactta caatcgtttt
     6601 cagtgtatca aagcgacgac tccctgtaaa cctcagccgc gataaggctg gtgagttagt
     6661 accatttagt gt
//
LOCUS       DAONFI010000094         6667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13441_length_6667_cov_4.224894, whole genome shotgun sequence.
ACCESSION   DAONFI010000094 DAONFI010000000
VERSION     DAONFI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13441_length_6667_cov_4.224894"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLK08_04255"
     CDS             complement(<1..248)
                     /locus_tag="PLK08_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52747.1"
                     /translation="MGSLPKKVFYLPGYNLVRILGHGAASTIWEVKCIETGRTFAVKR
                     VCRASKSDQRFVDQIKNEYQIASKISHSNIRKVYNLRIV"
     gene            659..1855
                     /locus_tag="PLK08_04260"
     CDS             659..1855
                     /locus_tag="PLK08_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPS52748.1"
                     /translation="MKVRNRILLITLKVLLAAALLGWVLSKAHWNDYIVSADGTSHVC
                     RGHDANGGLLVSQGMFDNTTKSVNPAAGGYKPIVQGGSEYIHKGLKSILIDINLWVLI
                     LAAVIYFVGLVLVGIRWWWLVRLQKLDMSLFDALSYTFLGLFFNYVIPGTIGGDLVKA
                     LYASKRTKHTAVLLVTIFTDRIIGIGAMAMLAIIMLGWVLLCGNVTVSQVKPAIIAIS
                     IISAIIVCGIVFVLSPKLRRMLFSHKLFSRMVISKHFEHAGNALNEYRRHPLAMICNS
                     MVGLMVHLTVFGSIALIGHSLLPNIPVYRYFIYLPIIYIIGVIPLTPGGVGFIESMYV
                     EFLAPTAAAVTGLFAVSLLARIIPIILSLPGLLVAVKGPKISTPDKLERQLEMAEELE
                     DDKRQQ"
     gene            1852..2337
                     /gene="folK"
                     /locus_tag="PLK08_04265"
     CDS             1852..2337
                     /gene="folK"
                     /locus_tag="PLK08_04265"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPS52749.1"
                     /translation="MKRVTAYIALGSNVGNRGQTLRDALDMMRRTSGITLKRVSAFME
                     TQPVGGPAGQGDYLNGVAEIETELTPQELLAALHAIESSLGRDRSRELRWGPRSCDLD
                     IIYYDDEIIDTPQLTIPHPRMHERSFVLGPLESIAPQVRHPLNFMTAGEMLRKLENIK
                     K"
     gene            2334..2933
                     /locus_tag="PLK08_04270"
     CDS             2334..2933
                     /locus_tag="PLK08_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPS52750.1"
                     /translation="MTAAARLISIIGAVGAGKTTLAELLSRQLDAVCIREDYAKNPFL
                     AESYMGNSELDLPSQLYFLTSRATQLNLTGWPESGLYVSDYGFSQDRIFAEWKLRGHD
                     LETYDFIAGKMARFVKQPDFMIHLDVDVEVLYNRIAARGRNYESVFTREFLEYLRQKQ
                     FDIEVPENCGFLRLDATAGDFRKPQVLAGIVDVIRAYRG"
     gene            3023..3892
                     /gene="panC"
                     /locus_tag="PLK08_04275"
     CDS             3023..3892
                     /gene="panC"
                     /locus_tag="PLK08_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPS52751.1"
                     /translation="MSIRVTRNIDEVRTAVRDARKEGRKVGLVPTMGALHEGHASLVR
                     AAARDCGMAVVSIYLNPTQFSPSEDLDKYPRTPDNDIKLCESCGASLVFMPSDEVMYP
                     AGKDAILTRIEVPSLAANLCGRNRPTHFAGVCLVVSKLFNIVQPDVAYFGQKDYQQAA
                     IIRQMVRDLDFPIKIEACPIVREADGLAMSSRNAYLSAEHRRQAAALSDALFAARDAI
                     LAGELPTNDDVTEFVADCIRTNAPAGRINYVQMVDAEDLHDIAFPVGNIVIAVAVKFG
                     QTRLIDNIYMELP"
     gene            3962..4324
                     /locus_tag="PLK08_04280"
     CDS             3962..4324
                     /locus_tag="PLK08_04280"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPS52752.1"
                     /translation="MTLKMLRAKIHRATVTEACVDYVGSITIDEDILDATGILPGECV
                     VVADVDNASRFETYVFRGSRGSGVFCVNGAAAHLVKVGHKIIIMAYAYLDETQAKTLK
                     PRVAVMDDANNIKEIMTY"
     gene            4340..5740
                     /locus_tag="PLK08_04285"
     CDS             4340..5740
                     /locus_tag="PLK08_04285"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPS52753.1"
                     /translation="MQQIIIDFGRFNIFGSELHLRIFGYGLMLVLGFICGILVARWRA
                     RRCGEDQEIVTQCGVLALAGGVVGARIFYVARHWADYAGSTNLIASLIDVSAGGLIYF
                     GGLAGGAIIVIIYLQFKKLPARRFLDIIAPSLMLGLAFGRMGCTLNGCCWGGPANQDW
                     LLGMKFPMISRPLLNFDGDSPYSPDQSTSPIYTDLYWQGLVTPDERLINTFVKQTRHL
                     EDQNRDSTRNMLIPLSEYHGQLNCNQVDVLLLPADEAAKLFQISSGGDSTISRHQFEK
                     SLADKTGILRGSESWSQAVAFDADGDGMLSFGEMQKYMQARLANLLQNFDADRNGHLS
                     AAERQAADSWLRQNMYDILRTQWTPKRRPAQVLGIINALLLFGLLNLFFRYRKCEGQV
                     FALMLILYPPTRFLLESVRDDDHFNLFHGNWTHNQISAWFIVAAGLVIWHLVKLRPSA
                     GPCALKRKELLHNKPK"
     gene            5867..>6667
                     /locus_tag="PLK08_04290"
     CDS             5867..>6667
                     /locus_tag="PLK08_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPS52754.1"
                     /translation="MKFDRRVLLSTIAAVAILVSSAAADEASSTIKRIAAATKNSIAL
                     IRCKTTDELGSRITVGTGISISPQIMMTYAVDSRIRTDKIESIEIIVPGVERQSFSGK
                     LLGVDPATGLSFVQVPSSYPWRPIAFAKSCKLELGDMVVSAGINIGDAELSQRLGCAY
                     IANLTRTPTMVYSVTGGTLTGPGSVVFNAAGQAIGLVTSQPYVDCEVMISRKNMNMPL
                     RNDEFTIAFLPVDEFVYILQQIPQDGKARRLPWIGVQRFAPVQKTLITA"
BASE COUNT         1648 a         1747 c         1712 g         1560 t
ORIGIN      
        1 acaatccgca ggttataaac tttacggata ttgctgtgcg atattttgga tgctatctgg
       61 tattcgtttt ttatctggtc aacgaatcgc tggtcggact ttgatgcccg gcagacacgt
      121 ttgacggcga aagtacgccc cgtttctatg cattttactt cccatatggt actggccgct
      181 ccgtggccga gtatccggac cagattgtac cctggcagat aaaacacctt tttcggcaaa
      241 gaacccatcg gatttaacac tcctttaaaa taccaccaat tcataaaaaa atgataatag
      301 ctcaactact caactataag tgaattactg ccagccaaaa cctcgaaatt acgaatgaca
      361 aaccctaaaa tctgtacttt gcgtcattcg gagaacgacg ccttttttac aattttccag
      421 tatgcgcaac cgtagttatg ctttttagcc gtcatatctt acgtacggga aacggttgga
      481 agcaactgtt ttcaggttgc tggcctccat aatgtcggca aaacagctta aaattgcgcc
      541 aaacagtttt ttaagtttat gtttttttgc atcaacattc aaccggcgcg tgcaaaaaaa
      601 attcttttgg catataatcc ccgcttatgc ataacttcgt gcaagtgaaa gaaatgccgt
      661 gaaagtacgc aacagaatac tcctgataac gcttaaggta ctgctcgcag ccgccctgct
      721 gggctgggtt ctatccaagg cgcactggaa tgattatata gtgtctgccg acggtacttc
      781 gcacgtctgc cgcgggcacg acgccaatgg cggcctgctg gtctcgcagg gaatgtttga
      841 caatacgacg aaatccgtaa acccagccgc cggcggatat aaaccaattg tccagggagg
      901 cagcgaatac atacacaaag gcttgaaatc gatacttatc gacatcaatt tatgggttct
      961 gatactggcg gctgtgattt atttcgtcgg gctggtgctg gtgggcatac gatggtggtg
     1021 gctggtcaga ttgcagaaac tcgacatgag cctttttgac gcgcttagct acacatttct
     1081 gggccttttt ttcaactacg tcatccccgg cacgataggc ggcgacctcg tcaaagccct
     1141 ctatgcctcc aaacgcacca aacataccgc cgtcctcctc gtcacgatat tcacggacag
     1201 aataatcggc atcggggcga tggcgatgct ggcaatcata atgctgggat gggttctatt
     1261 atgcggcaat gtaacggtca gccaggtcaa gcccgcaata atcgcaattt caataatatc
     1321 agccataatc gtctgcggga ttgtttttgt gctttcgccg aaactcagaa ggatgctgtt
     1381 ttcccataag ctctttagcc gcatggtgat atcaaaacat ttcgagcacg ccggcaacgc
     1441 tctgaacgaa tatcgccgtc atcctctggc gatgatatgc aattcgatgg tgggtctgat
     1501 ggtacattta acggttttcg gctcgatagc cctgataggc cattcactgc tgccgaatat
     1561 tccggtctac cgctacttca tatatctgcc tataatttac ataatagggg ttatcccgtt
     1621 aactcctggc ggcgtgggtt ttattgaaag catgtacgtt gagttcctgg cgccgaccgc
     1681 agcagccgtc accggccttt ttgcagtatc gctgcttgcc agaattatcc cgataatact
     1741 ctcgctaccc ggccttctgg tagcagtcaa aggcccaaaa atatccactc ccgacaaact
     1801 tgagcgacag cttgagatgg cggaggaact ggaggacgac aaacgacagc aatgaaacga
     1861 gtcacggcat atatcgcact aggcagcaac gtcggcaaca gaggccagac cctgcgcgac
     1921 gcgctcgaca tgatgcggcg cacttcgggc ataacgctga aacgtgtttc cgcgttcatg
     1981 gagactcagc ccgtcggcgg gccggctggc cagggcgatt atctcaacgg cgtagctgaa
     2041 atcgagaccg aacttactcc gcaagaattg ctggctgcat tacacgccat agaatcttcg
     2101 ctcggccgtg accgtagccg cgaactccgc tggggccctc gcagctgcga cctggatata
     2161 atatactacg acgacgaaat aatcgatacg ccgcaactca ccataccgca cccacgcatg
     2221 catgagcgaa gtttcgtact tgggccgctg gagtccatcg ccccgcaggt tcggcatcct
     2281 ctaaatttca tgacagccgg cgaaatgctc aggaagctgg agaatataaa aaaatgacgg
     2341 ctgccgcacg actcatcagt attatcggcg cggtaggcgc aggcaaaacc acactggcgg
     2401 aactgctcag ccgacaactc gacgcggtat gcatacgtga agattatgcg aaaaatcctt
     2461 ttctcgccga aagttatatg ggcaacagcg aactcgacct gccgtcccag ctgtatttcc
     2521 tgacatcccg cgcgacacag ttgaatctga caggctggcc ggaaagcggt ttatacgtaa
     2581 gcgattacgg cttttctcag gacaggattt tcgccgagtg gaaactcagg ggccatgacc
     2641 ttgagacata tgatttcatc gccggcaaga tggcccgatt cgtcaaacag ccggatttca
     2701 tgattcatct cgacgtcgat gtcgaagtcc tgtacaaccg catcgccgcg agaggccgaa
     2761 attatgaaag tgtttttacg cgggaatttc ttgaatatct gcggcaaaaa cagtttgata
     2821 tagaagtccc tgaaaattgc ggctttctgc ggctggacgc aactgccgga gatttccgaa
     2881 agccgcaagt tcttgcaggc attgttgatg taataagagc atatcgaggt tgattcgaca
     2941 ttgtgtcagt aacgacattg actgacgagg aaagcgaagc agctttcgat aataccctgt
     3001 atcagggatg aggttgtctg aaatgagcat acgcgtaaca cggaacatag atgaagttcg
     3061 cactgcggtg cgagatgcca ggaaggaagg cagaaaagtc ggtcttgtgc ccaccatggg
     3121 agcattgcac gaaggccatg cttcgctcgt gagagcagcc gcccgcgact gcggcatggc
     3181 tgtagtgagc atatatctga atccgacgca attttctcca tcggaggatt tggacaaata
     3241 cccaagaacg ccggacaacg atataaaact ctgtgaatca tgcggcgcgt cgttggtttt
     3301 catgccgtcc gacgaagtca tgtaccctgc cggcaaagac gccatactca ccaggataga
     3361 agttccgtct ctggcggcaa atctgtgcgg caggaatcgg ccgacgcact tcgccggcgt
     3421 atgcctggtt gtatcaaaac tcttcaacat agttcagccg gacgtcgcat atttcggcca
     3481 aaaggattac cagcaggcgg cgattatccg ccagatggtt cgcgatcttg attttccgat
     3541 aaaaatcgag gcctgtccca tagttcggga agcggacggc ctagccatgt ccagccgcaa
     3601 tgcctatctt tcggcggaac atcggcgaca ggctgcagca ctcagcgacg cactcttcgc
     3661 cgcgcgggac gccatactag ccggcgaact gcccaccaat gacgacgtca ccgaattcgt
     3721 cgcggattgc atccgcacca acgctccagc cggcagaata aattacgtcc agatggtaga
     3781 tgctgaagac ctgcacgata tagcttttcc tgtcggcaat atcgtcatcg ccgtagccgt
     3841 gaaattcggc caaacgaggc tgattgacaa tatttacatg gaactgccgt gatattttgt
     3901 tataattgac tgacttgaga tttttattga aaaaggattc gatgacaggg acttgcagca
     3961 aatgacacta aagatgctca gggcgaagat acaccgtgcc acggtcactg aagcgtgcgt
     4021 ggactacgtg ggcagtatca cgatagacga agacatactc gacgccaccg gcatactgcc
     4081 aggcgaatgc gtcgtggtag ccgacgtcga caatgccagc cgttttgaaa cctacgtttt
     4141 ccgcggcagc cgcggcagcg gcgtattctg cgtaaacggc gcagccgccc acctggtgaa
     4201 ggtcggccac aaaataataa tcatggcata cgcgtacctc gatgaaactc aggccaaaac
     4261 actcaaaccc agagtagccg tcatggatga cgccaacaac atcaaagaga taatgacata
     4321 ctgaccggaa aggattctga tgcagcagat tatcatcgat tttggccgat tcaacatatt
     4381 tggcagtgag ctgcatctgc gcatattcgg gtatggcctt atgctggtgc tcggattcat
     4441 ctgcgggatt ctggtggcga gatggcgagc caggcgatgc ggcgaagacc aggaaatcgt
     4501 gacgcaatgc ggcgtactgg cgttggcggg cggcgtagtg ggggcgagaa ttttttacgt
     4561 cgccaggcac tgggccgatt acgccggcag tacgaatctc atcgccagcc tgattgacgt
     4621 ctcagccggc ggccttatat acttcggcgg attagccggc ggagcaatca tcgtaattat
     4681 ctatcttcaa ttcaaaaaac tgccggctcg acgttttttg gacataatcg cccccagtct
     4741 catgttgggc cttgcattcg gccggatggg ctgcacgctc aacggctgct gctggggcgg
     4801 gccggcaaat caggattggc tgcttgggat gaaatttccc atgatttcaa ggccgctgtt
     4861 gaacttcgac ggcgattcgc cgtacagccc cgaccagtcg acgtcgccga tttataccga
     4921 cctttactgg cagggtcttg tcacgccgga tgaacggctg ataaacacat tcgtcaagca
     4981 gacccgtcat cttgaagacc agaaccgcga ctccacgcgg aacatgctca taccgctgtc
     5041 ggaatatcac ggccaactca actgcaacca ggttgacgtg cttctgctgc cggctgacga
     5101 agcggcaaag ctgtttcaaa tctcatccgg cggcgattcg acgatttcca ggcatcagtt
     5161 tgaaaaatcc cttgccgaca aaaccggcat acttcgcggc agcgagtcat ggtcgcaggc
     5221 tgtcgcgttc gacgccgacg gcgacgggat gttgagtttc ggtgaaatgc agaaatatat
     5281 gcaggccagg ctggcgaatc tgcttcaaaa cttcgacgcc gaccgcaacg gccacctgtc
     5341 agccgccgaa cgccaagccg ccgattcatg gctgcgtcag aatatgtacg acatcctcag
     5401 gactcagtgg acgccgaaac ggcggcctgc acaggtgttg ggcataataa acgccctgct
     5461 gcttttcggg ctattgaacc tgtttttccg ataccgcaaa tgcgagggac aggtttttgc
     5521 ccttatgctg atactttatc cgccgacccg tttcctgctt gaaagcgtcc gcgacgatga
     5581 ccattttaat ctttttcacg gaaattggac gcataatcag ataagtgcat ggttcatcgt
     5641 agccgccggc cttgtgatat ggcatctggt gaaactgcgg ccgtcagccg gcccatgtgc
     5701 attaaaacgc aaggaattat tgcataacaa acccaaatag ttaaacttgg agagtggttt
     5761 tcaaaaattt tttattttga gaacctggcg ttcaggatgt tgtcggacgg ttttgtacat
     5821 gcgcgtccga tgcaaggatt gaaaatcatc ttaaggagac ggtcatatga aatttgacag
     5881 gcgcgtcctt ctatcaacaa tagcagcagt tgcaattctg gtttcgtcgg cagccgccga
     5941 tgaggcgtct tccacaatca agcgcatcgc agcggctacg aagaattcca tagccctcat
     6001 ccgctgcaag actacggatg aacttggctc tcgcataacg gtgggtacgg gtataagcat
     6061 cagcccgcag ataatgatga cgtatgccgt cgattcccgc atccgcaccg ataaaatcga
     6121 atcaatagaa atcatcgtgc cgggtgtcga acgtcagagt ttttccggca agttgctggg
     6181 tgtggatcct gctacgggcc tcagtttcgt gcaggtgccc tcgtcatatc catggcggcc
     6241 gattgcgttt gcgaagtcct gcaaactcga actcggcgac atggtcgtat ccgctggaat
     6301 aaacatcggc gacgccgagc tgtcgcagcg tctcggctgt gcgtatatcg cgaatcttac
     6361 ccgcacgccg acgatggttt acagtgtaac gggcgggacg cttaccggcc ctggttctgt
     6421 ggtattcaat gccgccggcc aggctatagg actcgtgaca tcccagcctt acgtcgactg
     6481 cgaagtgatg ataagccgca aaaacatgaa catgccgctc aggaacgatg aatttaccat
     6541 cgcctttctg cccgtcgatg agttcgtcta cattcttcag cagatacctc aggacggcaa
     6601 agcccgtcgt ctgccgtgga taggcgtcca gcgtttcgct cccgtgcaga agacgctcat
     6661 aacagcc
//
LOCUS       DAONFI010000095         6626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13554_length_6626_cov_3.956475, whole genome shotgun sequence.
ACCESSION   DAONFI010000095 DAONFI010000000
VERSION     DAONFI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13554_length_6626_cov_3.956475"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..3037
                     /locus_tag="PLK08_04295"
     CDS             86..3037
                     /locus_tag="PLK08_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52755.1"
                     /translation="MKLPLTMLSVALSVVNAFGSPAGVRSIFISPDKKTCIAVGDCGL
                     AMKSGDCGKTWQPLKIPSADVNLQCVATARDGAETFLLGGEGVAGYPLGGIGRGVILH
                     SQFDEDSYKLNFSGITGWIYCGVFATTRGTLGGQAAAEALGGLYNTFSKGQIWLPLNV
                     SSRGYILGGAFDTWQSGYMVGSRRRIIKLQNLIEPKLNIPRIPSPWPLRAAAISQDKT
                     CWAVGDNGTILTSDKPDTPWPVRQISLPAGTALICDFECIDILGEKIAIAGGLNGVIF
                     ISSDGGVTFSPTPIPAGSPVRCIKWLNENTLLAGADCGTIWRSEDAGKTWQKTAGENQ
                     TDLLIIASATERGIYPVISEHSAAGVRVAVLFATVPNYGQNIPAEQQLRAAALNAGAS
                     AAAFLGDFESLTGEDFLDAMTETSLAEKWFKLLDIDPKQQMTSQIAAAIRLYRPRVVA
                     TGYYADGYRGKPAEIAFVARCTETAVELAAKKDAMQNFAQLNLPPHKVERLFKGCESN
                     ENTSTPWNAKSFGIDVKKYPFIIDTTRVVDGQNSPISYRSAFSAWLTGGGFLDRPAPK
                     IAYNSRDVQKRKVLLMTQGLTPRRLKLCRLNTDDSLLANGGLITFADGEGRLPVIMNE
                     LVGIFDEKDDGSGFDRKLLAADNMAMILYRLAAVGKLIPAVQAMQSFLEYGQMHPMYE
                     MLRVSSIATLSSSEFRGQMAMVQLDEVPQINRDFISQELPKLMKVSPWLTQPQGLAMA
                     ASLYRKIDQPGQSDHITKKMLDANFSPSWDSFVRTERAIRAGKIPESVGNRKILKPEV
                     IRGKMEIDGLLDDEAWQNATPLIMTGNDETHEELVGHAMLARNKTELVVALRLPNATA
                     RNWNVTLAIDSDRDAWVQEIIRFNSLGESSAQTVPFAGPAWTIDKRNILLKKYVASTS
                     DEQTFELVFPLKHFDAEIDSRDYWGFQIIAVADGPDGKTAIYLQPQDSPQLLPHRYGL
                     LDVRPQKINDK"
     gene            3422..4213
                     /locus_tag="PLK08_04300"
     CDS             3422..4213
                     /locus_tag="PLK08_04300"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HPS52756.1"
                     /translation="MRRRVKGDFVAAMLTISMLCCWACGDDSKKLVSSEDTFAHHSYP
                     DKTANAQIRLGVGYREETNLPYREGWTYLKFSLAAVPDDAVIASAVLYLVDSGNLPHN
                     SLEVGVYAVTDDSWSASTLTWNDKPDTAKTATANTTVSTVSTTNNQQLYSWDITSLVK
                     TNNIASDDVLSVCLKENPQQTTTDTWAMFVSTRDPDSNNHPYLVITYAPKADAKRGDC
                     SNDNLVSNHQQVTWSPLACLSWQGEFTGAQFSLAGSNNSHVNGHI"
     gene            4357..5067
                     /locus_tag="PLK08_04305"
     CDS             4357..5067
                     /locus_tag="PLK08_04305"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HPS52757.1"
                     /translation="MMRERKIHLRCVSLVILLLTILVVSNGLFGDTITLATSADTFAH
                     HSYPNNAANTATRLGVGYREEATLPYREGWIYLKFSLTSLPDDVVVTSAVLYLMENGN
                     LPNNSLTVSTYAVTDDSWSASTLTWNNKPDTAATSTSDTVISTTANLQWYSWNITSLV
                     STNNAEADDILSVCLKENPEQTTTDTWAMFVSTRDSNTSYYPYLVITYTNVPEPVTIS
                     LLGIGGIGLLFSKRSSHI"
     gene            5211..5897
                     /locus_tag="PLK08_04310"
     CDS             5211..5897
                     /locus_tag="PLK08_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS52758.1"
                     /translation="MQRQKLQSCRHQQSKSSAFTLIELLVVIAIISLLVSMLLPSLKT
                     AKALANRVVCSANMRNINLAFGLYNNDWDGWFPLVREVKNPLVSGVWADKLYDGTYLE
                     SAEAFNCPSVPLRVFIPDETNGACKMAYGMEWWVGGGDDIGQGHKIIDIVKPANTVLA
                     GENNNSSGAHGYGVHDNRWPVPLNEWGWPDDERHSGVSNILFVDGHVLAYTQDIACSD
                     SSIIWFTDGD"
     gene            5933..>6626
                     /locus_tag="PLK08_04315"
     CDS             5933..>6626
                     /locus_tag="PLK08_04315"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPS52759.1"
                     /translation="MYVTILNVKHLSKKSPLLQILLLALITSCPCALGTDEATEKNST
                     VSAKTDATPVMDKSIVVEQLGKANVILKADSLRVNDEKSFFSALNLDTPGMENVKKAV
                     SADDWPAAKKALLEYMRSRKPPKFSMDRWEKDEFVAFVKNEMSESIPQAIADADRVYR
                     HEFDWGCPYTFKGPIVWDKEKTGVTFSFGAMLSRMLFLNNLGVAWWLSNDKKYPLCAV
                     EIVNNFISSCPMP"
BASE COUNT         1919 a         1500 c         1559 g         1648 t
ORIGIN      
        1 cgccggataa ggacacgtcc tactgccccg ccggcaaaga cgccatgaac gtcataaacg
       61 attattatcg aaaattggac ggaaaatgaa actgccgctc acgatgctgt cagtcgccct
      121 gtcagtcgtc aatgccttcg gctcgccggc tggcgtgcga agtatcttca tatcgccaga
      181 caaaaaaacg tgcatcgccg tcggcgattg cgggctggcg atgaaaagcg gtgactgcgg
      241 caaaacgtgg cagccgctga aaataccgtc ggcagacgtg aatctgcaat gcgtagcaac
      301 cgcacgcgac ggcgctgaaa catttctgct cggcggcgaa ggggttgcag ggtatccgct
      361 gggaggcatc ggccgcggcg tcatactgca ttcgcaattc gacgaagatt cgtacaagtt
      421 aaatttttca ggcataaccg gctggattta ctgcggcgtt tttgcgacaa cacgcggcac
      481 tttaggcgga caagccgccg ctgaagcatt gggcggcctt tataacacat tcagcaaagg
      541 ccaaatatgg ctgccgctga acgtgtcaag ccgcggttat attctcggcg gtgctttcga
      601 cacctggcaa tcaggttaca tggtcggcag ccgacgcaga atcatcaaac tgcaaaatct
      661 tattgagccg aagttaaaca tcccacgcat accttcacca tggcccctgc gggctgctgc
      721 aatatcgcag gacaaaacct gttgggccgt cggagacaac ggaacgatac tcacaagcga
      781 caaacccgat acgccctggc ctgtaagaca gatatcactg ccggccggca cagcgttaat
      841 ctgcgatttc gagtgcatcg acattttggg cgaaaaaatc gccatcgccg gcggcttgaa
      901 tggcgtgatt ttcatcagtt cggacggcgg cgtgactttc agcccaacgc cgataccagc
      961 cggctcaccg gtgcgatgca tcaaatggct gaacgaaaat acgttgctgg ccggcgcgga
     1021 ctgcggaaca atctggcgaa gtgaagacgc aggaaaaaca tggcaaaaaa ccgcaggcga
     1081 aaatcaaacc gacctgctca taatcgcatc ggcaacggaa cgcggcatat atcccgtcat
     1141 cagcgaacac tccgccgcag gcgtaagggt agccgtactt ttcgcaaccg tgccgaacta
     1201 tggccaaaat attcccgcag aacagcaact gcgagctgca gccctgaacg ccggcgcatc
     1261 tgccgccgca ttcctgggag atttcgaatc gctgacgggc gaagactttc ttgatgccat
     1321 gacggaaaca tcgctagctg aaaaatggtt caaacttctg gatatcgacc ccaaacagca
     1381 gatgacgtcg caaatcgcag cagcgatacg gctatacagg ccaagagtcg tcgcgacagg
     1441 ttattacgcc gacggttacc gcggcaagcc ggctgaaatc gctttcgtcg cccgctgcac
     1501 cgaaaccgcc gtcgaactcg ccgccaaaaa agacgccatg cagaatttcg cccaactaaa
     1561 tctgccgccg cataaagtcg aacgattgtt taaaggctgc gaatcaaatg aaaacacatc
     1621 gacgccgtgg aatgcaaagt cattcggcat cgacgttaaa aaatatccgt tcattataga
     1681 tacaacccgc gtagtcgacg gccagaattc gccgatttca tacaggtcgg cattcagtgc
     1741 gtggctgacc ggcggcggct ttctcgacag gccggcgcca aaaatcgcgt ataattcacg
     1801 cgacgttcaa aaacgcaaag tgctgctgat gactcaaggc ctcacgccga ggcggctgaa
     1861 attgtgcaga ctcaataccg acgacagcct gcttgccaat ggcggactca taacttttgc
     1921 cgacggcgaa ggccgactgc cggtaataat gaacgaactt gtcggaatat tcgatgaaaa
     1981 agacgacggc tcaggtttcg acagaaaact gctcgccgcc gataacatgg cgatgattct
     2041 atatcgtctg gctgcggtcg gaaaactcat cccagccgtc caggcgatgc agtctttcct
     2101 ggaatacggc caaatgcatc cgatgtatga aatgctacgc gtatcatcca ttgcaacgct
     2161 cagttccagc gaattccgcg gccagatggc gatggtgcag ctcgatgaag ttccgcagat
     2221 aaacagggat ttcatctcgc aggaactgcc gaaactgatg aaggtttcgc cgtggctgac
     2281 ccagccgcaa ggcttggcga tggcggcaag cctatatcgt aaaatcgacc agccgggcca
     2341 atcggatcac attaccaaaa aaatgctcga cgcaaatttt tcgccatcat gggactcgtt
     2401 tgtcagaacc gaacgggcaa ttcgtgccgg caaaattccg gaatccgtcg gcaacagaaa
     2461 aattttaaag ccggaagtta tccgcgggaa aatggaaatc gacggcttgc tggacgatga
     2521 agcctggcag aacgccacgc cgctgataat gacgggtaat gacgaaacac acgaagaact
     2581 cgtcggtcac gccatgctcg cacgaaataa aacggaactt gtcgtagcgc tgcgtcttcc
     2641 caacgcaact gcacgcaact ggaatgtaac gctggcgatt gattccgacc gcgacgcatg
     2701 ggtgcaggaa attatcagat tcaactcgct gggagaatcg tccgcacaaa ctgttccctt
     2761 cgccggccca gcctggacga tagacaaacg taatattctt ctcaaaaaat acgtagcgtc
     2821 gaccagcgac gaacagacat ttgaattggt tttccctcta aaacactttg acgctgaaat
     2881 tgacagccgt gattattggg gctttcagat aatagccgtc gctgacggac ctgacgggaa
     2941 aacggcaatt taccttcagc cgcaggactc gcctcaatta ctgccccacc gttacggttt
     3001 gcttgatgtc cggccacaaa aaattaacga caaataggcg gttgctgcaa gtccttttaa
     3061 aaactaattt tatatcaatt ttccaaaaag ctctgttcga aaaaacctat gacggccggc
     3121 atatgcaact gtaaaattca tcttttttaa aaatacctcg cttttttgag aagtattttt
     3181 cttgacaaat cataacgttc tgattaatat tatggttgtt taataacgtt atgattaata
     3241 aagacgctac ggttttaatg tagtggcgaa taaaaatgag attcgtgaca caaatagcaa
     3301 aaacagcctg tcacggaata aaatgtagca taaatgatag taaaaagggt ttttaggcct
     3361 taaatacgat tgctgaactt tataaccatt ttttcgacta caattatgaa aggagtttac
     3421 gatgaggcgt agagtgaaag gtgattttgt tgctgccatg ctgacaattt cgatgctatg
     3481 ctgttgggca tgtggggatg atagcaaaaa acttgtgtcg tcggaagata cttttgcaca
     3541 tcattcttat cctgacaaga ccgcaaatgc tcaaataagg ttgggtgtag gctatagaga
     3601 ggaaacaaat ctgccatata gggaaggatg gacgtattta aaattcagtc ttgcggcagt
     3661 tcctgatgat gcagtaatag cctcagcagt tctttatctg gttgattccg gtaatcttcc
     3721 gcacaatagc cttgaagtag gtgtatatgc cgtcaccgat gatagctggt cagcttctac
     3781 tctgacatgg aatgataaac ccgataccgc taaaactgca accgcaaata caaccgtttc
     3841 gactgtttct actactaata atcagcagtt gtatagctgg gatataacgt cattggtgaa
     3901 aacaaacaac atcgcctctg atgatgtttt atccgtatgc ttaaaagaaa atccgcagca
     3961 aactacgacc gacacatggg cgatgttcgt ttcgacacga gatccagatt ccaataatca
     4021 tccatatttg gtgattacat acgcgcctaa agctgatgca aaacgcggag actgcagcaa
     4081 tgacaatctt gtatctaatc atcagcaagt aacctggagt ccattggcct gtctatcatg
     4141 gcaaggagag tttactggag ctcagttcag tttagcagga agtaataaca gccacgtaaa
     4201 cggccatata tagacgtgac ataattggcc gaattaatat acagttttgc gttatatgaa
     4261 taggccaata gtgattttta gtttaaattg cttatttatt gcattggtag ttttagataa
     4321 agagttctgt aaaacacaaa ttttagaaag gaattcatga tgagagaaag aaaaatccat
     4381 ttgcgatgtg tttcattggt tattttgctg ttaactattc tagtcgtaag taatggactg
     4441 tttggcgata ctataacact ggcaacatcg gcagatactt ttgcacatca ttcttatcct
     4501 aataatgccg caaataccgc aacaagatta ggcgtaggat atagagaaga agcaactttg
     4561 ccatatcgtg aaggatggat atatttgaag ttcagcctta cttcacttcc tgatgatgta
     4621 gtagtaacct cagcagttct ttatttaatg gaaaacggta atcttcccaa taatagcctt
     4681 actgtcagca cttatgcggt aacagatgac agctggtcag cttctactct gacgtggaat
     4741 aacaaacccg ataccgctgc gacatcgaca tctgacactg ttatttctac tactgctaat
     4801 ctgcaatggt acagttggaa tataacatca ttagtaagta cgaataatgc tgaagccgat
     4861 gatattttat ccgtatgttt gaaagaaaat cctgaacaaa caacgaccga tacatgggcg
     4921 atgttcgttt cgacacgtga ttccaatacc agttattatc cgtacctggt tattacctat
     4981 accaatgttc ctgaaccggt aacaatatcg ctgctgggaa ttgggggcat tggcctactg
     5041 ttttccaagc ggtcatctca tatttgaata atgtgtaagt caaaaggtat gcaaataccg
     5101 gtttgctaat ccggcatttg catacctcgt tgccattttt ataaatagcg gcgatagaaa
     5161 atatccggca tataagcgtt aaaacaatca agcagttgga ataacttact atgcagcggc
     5221 agaaattaca aagctgtagg catcagcagt caaaatcatc agcttttacg ttgattgaat
     5281 tgctggtagt aatcgctatt atttcactgc tggtgtcaat gttactgcca agcctgaaaa
     5341 ccgcaaaagc gttagccaat cgcgtagtct gttcggctaa tatgcgaaat ataaatttgg
     5401 cgttcgggct gtataataat gactgggacg gctggtttcc tcttgtcaga gaagtgaaaa
     5461 atccgctcgt aagtggcgta tgggctgata agttgtatga cggcacatat cttgaaagtg
     5521 ccgaagcatt caattgtcca agcgttccat tacgagtttt cattcctgat gaaactaatg
     5581 gggcatgtaa gatggcctac ggcatggaat ggtgggttgg gggcggcgat gatattgggc
     5641 aaggtcataa gattattgat atcgtcaaac cggcaaatac tgtcctggct ggagagaata
     5701 acaacagttc aggcgcgcat ggctacggcg tacacgacaa ccgatggccg gtgccattga
     5761 atgaatgggg atggcctgac gatgaacgtc attcaggcgt atcgaatata ctttttgtcg
     5821 acggccatgt tcttgcctat actcaggaca tcgcctgtag cgacagtagt ataatctggt
     5881 ttacagatgg cgattaatta atatactttt cacaaatgga gaaaatcatc taatgtacgt
     5941 taccatcctc aatgtaaaac atctgtcaaa aaaatccccc ctactacaaa ttcttttact
     6001 tgctttgatt acatcatgcc catgtgctct gggaacagat gaagccacag aaaaaaactc
     6061 aacagtatct gctaaaacag atgctacgcc tgtcatggat aagagcattg ttgtagaaca
     6121 gttgggaaaa gctaacgtta tattgaaggc tgattcacta agagtgaatg atgagaaatc
     6181 ttttttttca gcattaaatc tggatacgcc aggtatggaa aatgtcaaaa aagctgtatc
     6241 tgctgatgac tggccggccg cgaagaaagc tcttctggaa tatatgcgaa gccgtaaacc
     6301 acctaagttc tcaatggatc gttgggaaaa agacgaattt gttgcatttg tgaaaaatga
     6361 aatgtcagag tcaattccac aggctattgc cgatgctgat cgtgtatata gacatgagtt
     6421 tgattggggt tgtccttaca catttaaagg gccgattgta tgggacaaag aaaaaactgg
     6481 agtaacattc agttttggcg ccatgttaag ccgtatgttg tttttaaata atttaggtgt
     6541 agcctggtgg ttgagcaacg ataaaaaata tcctctatgt gccgttgaga tagtgaataa
     6601 tttcatcagt tcctgcccta tgcctc
//
LOCUS       DAONFI010000096         6588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13661_length_6588_cov_4.314710, whole genome shotgun sequence.
ACCESSION   DAONFI010000096 DAONFI010000000
VERSION     DAONFI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13661_length_6588_cov_4.314710"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..555)
                     /gene="rnhA"
                     /locus_tag="PLK08_04320"
     CDS             complement(76..555)
                     /gene="rnhA"
                     /locus_tag="PLK08_04320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPS52760.1"
                     /translation="MKTLFNPEKPSSCDTVDIYTDGGCSPNPGPGGWAAVLISEKHDL
                     KKEISGAECDTTNNRMELLAAIQALRTLKKPCNVKLHTDSQYLQKAFCDGWLDNWVKK
                     GWKTAGKKPVANQDLWQELLKLTAIHNVDFVWVKGHAGNDYNERCDELVNLARQTMS"
     gene            complement(581..1357)
                     /locus_tag="PLK08_04325"
     CDS             complement(581..1357)
                     /locus_tag="PLK08_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPS52761.1"
                     /translation="MEKLRAKEAFICDMDGVIYHGNKLLPGVVEFVGWLRNEGKKFVF
                     LTNSSERSPRELREKLGRLGLDVAEGHFFTSAMATAEFLARQCPGGSAYVIGETGLIN
                     ALYNAGFSMNDSNPDYVVMGETRSYSYEKLEKAVNLVRCGAKLIGTNPDLTGPTESGI
                     APATGSLISPIELSTGTKAYFVGKPNPLMMRNALKKLCAVRETTAIIGDRMDTDVLAG
                     MEAEIETVLVLSGVTSREDLPKFSYRPGYILHGVGDIVSS"
     gene            complement(1366..2121)
                     /locus_tag="PLK08_04330"
     CDS             complement(1366..2121)
                     /locus_tag="PLK08_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPS52762.1"
                     /translation="MSGHSKWANIKHRKGRQDEKRGKLWSKIARTIIMAAKAGGGDPA
                     GNLTLRYAIDEAKEANMPKETILNAIKKGTGELGAENYEEVVYEGYGPCGVAFMVYAL
                     TDNRQRTGPEMRKIFERAGGQLGATNCVAYLFTQKGMLSIPADKADEDTLMEIAINAG
                     ADDVTREGDFFEITCEPDVFAAVKDALAAKGITPSSSQLAMIASNTVTPSVEKTRQVL
                     NLTEQFEDHDDVQKVFTNLDIPDELMKELENEG"
     gene            complement(2152..5772)
                     /locus_tag="PLK08_04335"
     CDS             complement(2152..5772)
                     /locus_tag="PLK08_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS52763.1"
                     /translation="MRNGRRGAEVKIFGKIIVKGCLTTAMVASLMLSAGCENSKKTIA
                     AEKTTAAVDRPAELPATAYVPLDKVSPEIVFKSFPQTRLPENDSIKKTVGKFLAAGMK
                     FQVQGNLDQAMRQYESARMYAPDSPRVLKALGMIWMAKREYEKAANYFRMSLDVDPDD
                     LQVQVNLGRVYLMQDKPEEALYCFRAALRTKQAKPDNIETAYAALYRSRLLEKLGYYT
                     AALQSYDYFETLVRQNWASYQKDSVIGDFILKPERFMIARGNIYDLLRQPQKALEEYK
                     KALARDRNAVKVGIKIFEIYVQTGDIKNAEKLLLEFAMQEDYSSYMDKLAVILCEKSH
                     DENQPLRLWKEFRRHNAMSIVFAFAMAEAADAIHAWPQEKTILTDIINMLPDAPQAAE
                     MLAELAIKTGHPEECMEQMAGLIVNNTKAFDNVSAAMEIISQLDDAGLLLDKFSQIPA
                     ANNLSIQAGRNYVAAILAAKLQQADRSNRLLEEAIKIDPKFSPAYVSLLQPQWIRQNS
                     SKTDEYVKRMKANTVGQDYFYVLGEVELARGNYSAAVASLLKARELDESSEPVIMKLS
                     QAYELEARKTTDSTKAAQYNQNSEELLQTAIELRPDKLEAYRRLFEKYVNRLDTRDAA
                     DIAIQVIKNSPQSPEGRIMAAETYLIANNRSRAKIIIDDLIKNCPDDPQVSLLQIRFT
                     LGEHEGLISKKSFDTNYAAIKRLIANHPDFVPAYELAGRLLGMAMPADYDEAVKYYGQ
                     AYRLAPNDFSCAREYVLAMLRAGDYKTALPLAETLLAESPRSITLRQAIISCLDKMGD
                     DKKALQYLDAWHKAAPSNKTYIEWILKLYVKGKQYGQAVKMLDSLSEVSGFPQTWIAD
                     RKFDLYMSNNDFKNAFEIVKQEQLSDVMRSMILLMDAMANAKKYDQALKFIDELRTVG
                     KENETRARQLSGLRYYILSKADRLSEFMADFSKEKPTPAVCAGVYAALMSGKHYDEAL
                     KLLDKWQIKESFSPDDTEKTQINSWCREMIVATYLAQSDYDKASKALQDSMATDGEKS
                     WYYYYNATVLMHNGENDDAVLMLQKALNEDPKNAMLKNDLAYQLAVIGRDLKNAEKLA
                     RECLQAKGFEPAYMDTLAWVFFKDNQPGRAGEILLKVVPSPEKINAMLAKAKDENRRL
                     DAEEINPVIVGHLGDVLAELGWTNTAREYWQAAIDLAQQWPTKTDDIKLMLKDASAKL
                     KK"
     gene            complement(5853..>6588)
                     /gene="hisG"
                     /locus_tag="PLK08_04340"
     CDS             complement(5853..>6588)
                     /gene="hisG"
                     /locus_tag="PLK08_04340"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPS52764.1"
                     /translation="CVMFRAQEMSRYVEEGIVDLGLTGYDWICENNSDVVEICELVYS
                     KATSKPACWVLAVPEESPIYKVEDLNGGSVSTELMNVTKKYFADKGVKVNVEFSWGAT
                     EVKARLLDGIVDVTETGSSIKANKLRIIDTLLTSTPRLIANKKTMEVDWKRKKIEDIA
                     MLLKGAIEAKGKVGLKMNAPKNRLNEISAVLPSEKSPTVSQLADKDWVAVEVIVDEIT
                     DRNLIPQLKRLGATGILTYPLNTIIH"
BASE COUNT         1376 a         1718 c         1615 g         1879 t
ORIGIN      
        1 ctcgtcctca acgacgcaac aaggcgcaat ttcaaattta acatgctcta aaaaactgac
       61 ggcggcacag ttacgctatg acatcgtctg ccgcgcaaga tttacaagtt cgtcgcaacg
      121 ttcattgtaa tcattaccgg cgtggccttt gacccagacg aaatcgacat tgtgaatagc
      181 cgtcaatttc agaagttcct gccataaatc ctggtttgcg acaggttttt tgcccgcggt
      241 cttccaccct tttttcaccc agttatccag ccagccgtca cagaacgctt tttgcagata
      301 ttgagaatcc gtgtgcaatt ttacattaca cggctttttg agagtgcgca gagcctgaat
      361 ggcggcaagc agttccatac gattatttgt cgtatcgcat tcggcgccgg aaatttcttt
      421 cttcaaatcg tgtttttctg aaatcagaac cgctgcccag ccgccagggc ctggattcgg
      481 cgaacagcca ccatccgtat aaatatccac agtatcgcat gaagaaggtt tttcggggtt
      541 gaaaagagtt ttcattgtcg tctcgtatct gtagcaaaaa ttatgacgat acgatatcgc
      601 cgaccccgtg caggatatag ccaggcctgt aagaaaactt cggcaggtct tctcgcgatg
      661 tcacaccgct gagcaccaga acggtttcaa tttcagcttc catgccggcc agcacgtcgg
      721 tgtccatgcg gtcgcctatg atagccgtcg tttcccgcac ggcgcagagt tttttaagtg
      781 cgttgcgcat catcaacgga ttgggcttgc cgacaaaata cgctttcgtg ccggtcgaaa
      841 gttcgatagg cgagataagc gagccagtag ctggggcaat gccactttcc gtcggaccag
      901 tcaggtcagg gtttgtgccg atgagttttg ctccgcatcg tacaaggttg accgcctttt
      961 ccagtttttc ataactgtag ctgcgcgtct cgcccatgac tacataatcg ggattggaat
     1021 cgttcatcga aaagccggcg ttgtagaggg cgttgataag gccggtctcc ccgattacat
     1081 acgccgagcc gcccggacat tgtcgcgcaa gaaactcggc agtcgccatg gcactcgtaa
     1141 aaaagtgccc ttcagccacg tcaaggccca gccggccaag tttttcccgc aattcgcggg
     1201 gagaccgttc ggatgaattg gtaagaaaca cgaatttctt gccttcgttt cttagccatc
     1261 cgacgaattc caccacgcct ggcagcagtt tattgccgtg ataaatcacg ccgtccatat
     1321 cgcatatgaa cgcttctttg gcacgcagct tttccatatt ctttctcagc cttcgttttc
     1381 cagttctttc ataagttcat ctggaatatc cagattcgta aaaaccttct gaacgtcgtc
     1441 gtggtcttca aactgttccg tgaggttgag cacctgtcgc gttttttcga cgctcggcgt
     1501 aacggtattc gacgctatca tcgccagctg ggatgaagac ggagttatgc ctttggcggc
     1561 taaggcgtct tttacggcgg caaagacatc cggctcgcag gtaatttcga aaaaatcacc
     1621 ttcgcgggtc acatcgtcgg cgccggcgtt tatcgctatt tccattagcg tgtcttcgtc
     1681 cgccttgtca gccggtattg acaacatgcc tttctgcgtg aacagatacg caacgcaatt
     1741 ggttgcaccg agctggccgc cggctcgttc gaatattttt ctcatttcgg gaccggttcg
     1801 ctgacgattg tccgtaagag catagaccat gaacgccacg ccgcaggggc cgtaaccttc
     1861 gtataccact tcttcgtaat tttccgcccc aagttcgccg gttccttttt tgattgcatt
     1921 caggatggtt tccttgggca tgttggcttc tttcgcctca tctatcgcgt agcgcagggt
     1981 gagattgccg gcaggatcgc cgccgccggc tttggcagcc atgattattg ttctggctat
     2041 tttcgaccag agtttgccgc gtttttcgtc ctgccggcct ttgcggtgtt tgatattcgc
     2101 ccatttacta tgtccagaca tataccgaaa tcctttcgta catcataaaa attatttctt
     2161 cagtttcgct gaagcatctt ttaacatcag ttttatgtcg tcggtttttg tcggccactg
     2221 ctgtgcaagg tcgatagcag cctgccagta ttcccttgcg gtattcgtcc agcccagctc
     2281 tgccagaaca tcgccgagat ggccgactat caccggattg atttcttcgg catcaagcct
     2341 gcgattttca tctttagcct tagccagcat cgcgttgatt ttttccggcg acggcacaac
     2401 tttcaaaagt atttcgccgg ctcggcccgg ctgattatcc ttgaagaaaa cccacgcgag
     2461 cgtatccatg tatgccggct cgaagccttt tgcctgcagg cattcgcgcg caagtttctc
     2521 ggcgtttttg agatcacggc caattacagc aagctgatac gccaggtcgt ttttcagcat
     2581 ggcgtttttg gggtcttcat ttaacgcttt ctgcaacatc agaacggcat cgtcgttttc
     2641 gccgttgtgc atgagaaccg tcgcgttata gtagtaatac catgattttt cgccgtcggt
     2701 ggccatgctg tcctggagag ccttggaggc cttgtcgtaa tcggactggg ccagataggt
     2761 cgccacaatc atttcgcgac accagctgtt gatttgcgtc ttttcagtgt cgtcgggact
     2821 gaaggattcc tttatctgcc atttgtcgag cagcttaagc gcttcgtcat aatgttttcc
     2881 cgacatgagc gcggcataaa cgccggcaca gacggcgggg gttggttttt cttttgaaaa
     2941 gtcagccatg aactcactga gccggtctgc cttgctgagt atgtaatacc gtaagccgct
     3001 gagctgtctg gcccgcgttt cgttttcttt gccgacagtt ctaagttcat ctataaactt
     3061 cagcgcctgg tcgtactttt tcgcgttagc cattgcatcc ataagaagta tcatgcttcg
     3121 catgacgtcc gatagctgtt cctgcttcac gatttcaaaa gcgttcttga agtcgttgtt
     3181 cgacatgtac aggtcgaact ttctgtccgc tatccaggtt tggggaaaac cggaaacttc
     3241 cgaaaggctg tcgagcattt taacggcttg gccgtattgt tttcccttga cgtaaagttt
     3301 cagtatccat tcaatgtagg ttttgttaga cggcgccgcc ttatgccacg catcaaggta
     3361 ttgcagggct tttttatcat cgcccatctt gtccaggcat gagattatcg cctgtctaag
     3421 agttatgctt ctgggtgatt cagccaaaag tgtttcggcg agcggcagag ctgttttgta
     3481 atcgccagct cgcaacatcg ccagaacgta ttcgcgggca cacgaaaaat cattcggcgc
     3541 cagcctgtaa gcctggccgt aatatttcac ggcttcatcg taatcagccg gcatggccat
     3601 gcccaacagt cgaccggcga gttcgtatgc cggaacgaaa tcaggatgat ttgcaatcag
     3661 ccgcttaatc gctgcgtagt tggtatcgaa tgattttttt gatatcaagc cttcatgttc
     3721 gcccagagtg aaacgtatct ggagcaggct gacctgcgga tcatccggac aattttttat
     3781 aaggtcgtca attattattt ttgcgcggct gcgattattg gcgatgagat acgtctccgc
     3841 cgccattatc ctgccttccg ggctttgcgg cgagtttttt atgacctgga ttgcaatatc
     3901 cgccgcgtca cgggtatcca gccggttgac gtatttttca aaaagacggc gatatgcttc
     3961 cagtttatcc ggccgcaatt cgatagccgt ctgaagcagt tcttcggaat tctggttata
     4021 ttgcgcagct tttgtggaat cggttgtttt acgggcttca agttcgtacg cctgcgaaag
     4081 tttcattatg accggctcgc tggattcgtc cagttcacga gctttcagaa ggcttgcgac
     4141 ggcggcggag tagttgcctc tggcgagctc aacttcgccg agcacgtaaa aataatcctg
     4201 gccaacggta ttagccttca tcctcttgac gtattcatcg gttttcgacg aattttgacg
     4261 tatccactgc ggctgcagaa gcgatacata cgccggcgag aatttggggt ctattttgat
     4321 tgcctcttcc agcagcctgt tgctgcggtc agcctgctgg agtttggcgg cgagaatcgc
     4381 ggcgacataa tttcggccag cctgtatcga caggttattc gcagcaggta tttgcgaaaa
     4441 tttatccaac agcagcccgg catcgtccag ttgcgatatt atttccatcg cagccgagac
     4501 gttgtcgaac gccttggtat tattgactat cagtccggcc atctgttcca tgcattcttc
     4561 cggatggccg gtttttatcg caagttctgc gagcatttcc gccgcctgtg gtgcgtcggg
     4621 cagcatgttg atgatatcgg ttagaatagt tttttcctgc ggccaggcat gaatggcgtc
     4681 agccgcctcc gccatcgcga atgcgaaaac aatcgacatg gcgttatgac gacgaaactc
     4741 cttccacagc cgaagcggct gattctcgtc atgcgatttt tcgcacagaa tcaccgccag
     4801 cttgtccatg tagctcgaat aatcttcctg catggcgaat tccagcagga gtttttcggc
     4861 gtttttgatg tcgccggtct ggacgtatat ttcaaaaatc tttatgccga ccttgactgc
     4921 attacggtcg cgtgcgaggg cttttttata ttcctccagc gctttttgcg gctgtcgcag
     4981 caggtcataa atattgccgc gcgctatcat gaaacgctcc ggcttgagaa tgaaatcgcc
     5041 gattacggaa tctttctgat agctggccca gttctgacgg acgagcgttt caaaataatc
     5101 gtaactttgc agggctgcgg tatagtagcc cagtttttcc agcagccgcg aacggtacag
     5161 cgcggcataa gcggtttcga tgttatccgg cttggcctgt ttcgttcgca acgccgcgcg
     5221 gaaacaatat agtgcctctt cgggcttgtc ctgcatcaga tacaccctgc caagatttac
     5281 ctgtacctga agatcgtcgg ggtctacgtc gagcgacatg cggaaataat tggcggcttt
     5341 ttcatattcg cgcttggcca tccatatcat gcccagcgcc ttgagcaccc gcggactgtc
     5401 cggtgcatac attcgggccg attcgtattg tcgcatcgcc tgatccagat tgccctggac
     5461 ctgaaatttc atgccggcag ccaggaattt tcctaccgtt tttttgatgg aatcattttc
     5521 cggcagacgg gtttgcggaa aagatttgaa aactatttca ggcgaaactt tatccagcgg
     5581 cacatatgca gtagccggca gttcagccgg cctgtcgacg gccgctgtag ttttttcggc
     5641 agctatcgtt tttttggaat tctcacagcc ggctgacaac atcaacgatg caaccatcgc
     5701 cgtagtcagg caacctttta cgattatttt cccaaaaatc ttcacttccg cacctctgcg
     5761 tccgtttcgc acatggtttc cgtttaacgc atatttttaa gtgcatttat gccgacggcc
     5821 tgctgaaaag caggccgcca gaagtcgacg tctcagtgta ttatcgtatt gagcggatag
     5881 gtcaaaatgc cggttgcgcc aagtctttta agctgtggta tcaaattgcg gtcggtaatt
     5941 tcgtccacta tgacttccac agccacccaa tctttgtcgg ctaactgcga aacggtgggg
     6001 cttttttccg aaggcaggac ggcggatatc tcattcagcc ggtttttggg ggcattcatt
     6061 ttcagcccga ctttgccctt ggcttcaata gctcctttaa gcagcatggc gatatcttct
     6121 atttttttcc gcttccagtc cacttccatg gtttttttat tggcgataag cctcggcgtg
     6181 ctggtcagca acgtatcgat gatacggagt ttgttggctt tgatgctgct gccggtctcc
     6241 gtaacatcga cgataccgtc cagcagccgc gctttgacct cagtggcgcc ccagctgaac
     6301 tcgacgttga cctttacgcc cttgtcggcg aaatattttt tagtcacgtt catgagttct
     6361 gtggagactg aaccgccgtt caggtcttct accttgtata ttgggctttc ttcgggaacg
     6421 gcgagtaccc agcaggctgg tttggaggtg gctttggaat agaccagttc acatatttcc
     6481 accacgtcgg aattattttc gcatatccag tcatatccgg tcaggccgag gtcaactatg
     6541 ccttcctcta catagcggga catctcctga gcgcgaaaca tcacgcat
//
LOCUS       DAONFI010000097         6583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13673_length_6583_cov_3.986979, whole genome shotgun sequence.
ACCESSION   DAONFI010000097 DAONFI010000000
VERSION     DAONFI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13673_length_6583_cov_3.986979"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..759)
                     /locus_tag="PLK08_04345"
     CDS             complement(106..759)
                     /locus_tag="PLK08_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPS52765.1"
                     /translation="MGTYSTSEKTREALITAAGELVAKHGFAKVSTRAIAEKAGENIS
                     SIHYHFGGKEQLMKEVVRTATKRHTENPTSEIIKSFENVLYTPEGQSKAVRALVNRFI
                     DVIFSPENPWWYSYVPYQIMKSENSLQILIKAGVIDDDFASLMNFFKIIKPDFTQEQL
                     KLYIKIFLAPIIIHTQNIEKGLLYNEHQYSRDYLSQLEEIAVKMNHCLLGLPYQENT"
     gene            1021..3876
                     /locus_tag="PLK08_04350"
     CDS             1021..3876
                     /locus_tag="PLK08_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52766.1"
                     /translation="MRFCCKIIVAITLSAWLGGCEPWNFGGGEAPASDKPPAVWCASD
                     MTPLTDRTEQFTDNDIYNSTEELVDISAAANETVSFQLIVDAADTGLAALKLDTTDFG
                     KRDGTTLEIESVSIFRMIPTPVDEYPAWYLRISPEKPVRANMYDQLVPINAPKDGQPY
                     RLNPHSRECFWVDIAVPAGADSGVYRGTIKISDGEKEIRRVVVQLTVDGFVLPSLRLL
                     PAIGSFGHRELITSFMLGDDGKPYDPAWLNPQNRQGRQAISLINNLIQLAHAHHLDIF
                     DTDLRPLIKRDDRGYLKLDWTGFDAVAGAYLTGKAFRDETPLPAWPIPFDSTYPDPAN
                     YGGINSIPYRLAVREYILQSARHLRSCGNDDQVFAWPYRRGINANAFENFTTLAEIVR
                     RADDSLPILCSLPLELPVGNTWKIPSDMKKYCDIVAPGGAWFDPSKHANRSKPANPLI
                     GTWLSPGNPPYMPDMSLFASPADIRALPWFALKYKCSALFFGNVLNWQDPKSSANLFY
                     SGRRWGIDDALPSVRLKRLRRGMQDIAYLWILRQRGREKQARKVIDMMVRYGGLAAAG
                     DNYLDARLDGWTAQGTTWNSARDLLADEIMQTINPQKQPDAAQAIAQKVRWKHLADAT
                     ETLRAERIRTKIRPSRKGQNQQTQTKLFADVSVDFFNEYPTPARGAMHVLRLPFGWKR
                     VQDEVSYNIAPGGRKTLAITIEGEKLPYIANGKIPVDIRVNTDQAEPQTLLTEVPIVV
                     AGDFKSAPTIDGNLADWPEATNTAGDFRLLGRRGETGSGLAERQTTVYTMQDDDNIYF
                     AFYCKEASPQSLRANAANTIRYDKLLACGEDYVEVILAPGGEAKKPQDLYHIMVKPNG
                     VVIQEHGVGCTPPLGKAQREDFGTVAAVSRGDDFWIAEIKVPRKNFGEHGDEPFWGVN
                     FARFATSTQEASSWAGAERYFYDPQSLGTLILKKTEN"
     gene            3950..4969
                     /locus_tag="PLK08_04355"
     CDS             3950..4969
                     /locus_tag="PLK08_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52767.1"
                     /translation="MATKISWLVGILLIVGFLGFLGMRLFSPRPDAPTTATTKAGVLE
                     IIKPAMPISEILGSQPSGKNNAGEYYNDAVLYFRENKKDFKEIYNQMTAGTENPQMFG
                     WQEDLCLNLQKKLAPAAECETMKYTLLFTPDKLTVSGKPDGQDDLYWLKNMLELLCMS
                     YQHDSQWQKAVESAKTMLLLGWHMTQDDSRAMYQITGLDIQADAAQRLAFYYGKLGNT
                     KMSKLCDDYYNQAITAGTLAHEKYNNVWNLRQRPGGKQGPFPGDLFNIIENDQSRGWK
                     VDALLAMSIAKLQEAKTKGNHAEIERLYDKYSQCNDPLLRAAAKASQTATKDDIENAM
                     RGDSE"
     gene            4984..5622
                     /locus_tag="PLK08_04360"
     CDS             4984..5622
                     /locus_tag="PLK08_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HPS52768.1"
                     /translation="MKTYPAMLNLSGRLVAVIGAGTTGLRKVRSLCDAGAKVRLIAGK
                     NFNPGCELPDDVEVLAEDFDSKTSTRLLEDCRLIFACCNDRKTNSEIAAVSRKLGIWV
                     NVADQPEDCDFFCPAVLCNGDVTIAIGTGGNAPGLSAELKKFISRNLPADTGRFAESL
                     SHLRAELKNSCHDPSARQAAMKKLSSEEGYAAFASRGEHGLREMLSGLQGGN"
     gene            5625..>6583
                     /gene="hemA"
                     /locus_tag="PLK08_04365"
     CDS             5625..>6583
                     /gene="hemA"
                     /locus_tag="PLK08_04365"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPS52769.1"
                     /translation="MLVICFGVNHKTAELSLREKFALSPTQLDNKLRELFEKFPAAEF
                     LLLSTCNRTELYIIRSLHGHPRNEEVTEFFSAMTGLEQSAFAESLYVLQDSEAVAQMF
                     AVAAGLDSMVPGESQIVSQLKQALAAAQRLQVARANMTTLVAEALHVAKHVRSETGIA
                     QGKVSVASVALELIANRFKTLAGMTVLSVGAGKMNEIILQTLRTMLPSELLITNRSPE
                     KATELAESCGGKIVPFEALAELVGRSDIIITSTASQSPIITCDMLASLPPKERLFVDM
                     AVPRDIQADAELLDGVELYNIDDLNGTVSQTLDERFKHVEQAE"
BASE COUNT         1730 a         1758 c         1644 g         1451 t
ORIGIN      
        1 acataagcac cgtaaaaaca cggatacttc tcttttttac aatattttca ctcatatgcg
       61 tttcctttac ttctttatca gagtattaaa atcaaaaaac ttcccctacg tattttcctg
      121 atatggcaga cccaataagc agtgattcat ttttactgca atttcttcaa gttggctaag
      181 ataatcgcga ctgtactgat gttcgttata aagcaggcct ttctcaatat tctgggtatg
      241 gatgattatc ggagccaaaa atatttttat gtaaagtttc aactgttcct gcgtaaaatc
      301 aggttttata attttgaaaa aattcattaa cgatgcgaaa tcatcatcaa tcaccccagc
      361 ctttataagt atctgaagcg aattttccga tttcattatc tgatacggaa catatgaata
      421 ccaccatgga ttttccgggc tgaaaatcac gtctatgaac cgattcacaa gcgcccttac
      481 cgctttggat tgtccttccg gcgtatataa aacgttttcg aatgacttta taatctcgga
      541 ggtagggttc tctgtatgcc tttttgtggc agttctgaca acctctttca taagttgttc
      601 tttgccgcca aaatggtaat gtatactgct tatattctct ccagcctttt cagctatggc
      661 tcgcgtcgaa actttcgcga aaccgtgttt ggcgaccagt tcgccggcag cggttatcag
      721 tgcttctcgc gttttttcac ttgttgaata tgttcccatg ctgttatttc caatcaaatt
      781 tcaatcattt ggcataatta tatcaagtgg cctaattttg tcaagaattt ttccaatatt
      841 tttattggtc acattttagc cgcctgctat atcattcgtt atacggctta cgcctagaaa
      901 atccgcagaa aacatcgaca ttacatttta aatagtgaaa tggcgtcaat ttgacggatt
      961 catttcggat tgccgattaa actgcttgga aattaccggc tgagtcagta caatttctct
     1021 atgcgttttt gttgtaaaat cattgtcgca attacgttgt cggcgtggct cggcgggtgt
     1081 gagccgtgga acttcggcgg cggagaagcc cccgccagcg acaaaccccc tgccgtgtgg
     1141 tgcgcatccg acatgacacc cctgactgac cgcactgaac aattcactga caacgacatc
     1201 tataattcga cagaagaact ggtggacatt tccgcggcgg cgaacgaaac cgtcagtttc
     1261 cagctaatcg tcgatgccgc cgataccggc ctggctgccc tgaaactcga cacaaccgac
     1321 ttcggcaaac gcgacggcac aacacttgaa attgaaagcg tctccatatt ccgtatgatt
     1381 ccgacgcccg tggacgaata tcccgcgtgg tatcttcgca taagccctga aaaacccgtc
     1441 agggccaaca tgtacgacca acttgttccg ataaacgccc ccaaagacgg ccagccgtat
     1501 cgcctcaacc cccacagccg cgagtgtttc tgggtggata tcgccgtgcc ggcgggcgcg
     1561 gattccggcg tttatcgcgg cacaataaaa atttcagacg gcgaaaaaga aatcagacgc
     1621 gtcgtcgtac aactgacggt cgacgggttt gttctgccgt cgttgcgact tctgcccgcc
     1681 atcggttcgt tcggccatcg tgaattgata acatctttca tgctcggcga cgacggcaaa
     1741 ccgtacgacc ctgcatggct gaatccgcaa aaccggcaag gccgtcaggc tatcagcctc
     1801 ataaacaatc tcatccagct ggcacacgcg catcatctgg atattttcga caccgacctc
     1861 cgtccgctga taaaacgtga cgaccgcggt tacctcaaac tggactggac gggttttgac
     1921 gcggtagccg gtgcgtatct cacgggcaag gcgtttcgcg acgaaactcc gctgccggct
     1981 tggccgatac cgttcgacag tacctatccc gacccggcca attacggcgg aataaattcc
     2041 ataccgtatc gcctggcggt tcgcgaatac atattgcagt cagccaggca tctccgctcg
     2101 tgcggtaatg acgaccaggt attcgcatgg ccgtatcgcc gcggcataaa cgcaaatgcg
     2161 ttcgaaaact tcaccacgct ggcggaaatc gtacgtcgag ccgacgactc tctgccgata
     2221 ctctgctccc tgccgctgga attgccagtg ggcaacacat ggaaaatccc gtccgacatg
     2281 aaaaaatact gcgacatcgt cgcccccggc ggcgcctggt tcgatccgtc gaaacatgcc
     2341 aaccgctcta aacccgccaa tccgctcatc ggtacatggc tgtcgcctgg caatccgcca
     2401 tatatgccgg acatgagcct tttcgcctcg cctgccgaca tccgagcact gccgtggttc
     2461 gcactgaaat acaaatgttc cgcattgttt ttcggcaatg tacttaactg gcaggacccc
     2521 aaaagcagtg ccaatctttt ctattccggc cgcagatggg gcattgacga cgccctgccg
     2581 tccgtgcggc tgaagcggct gcgaagaggc atgcaggaca ttgcgtatct gtggattctc
     2641 cgccagagag gccgtgaaaa gcaggctcgt aaagtcatcg acatgatggt acgttacggc
     2701 ggtttggcgg cggcaggcga caattatctc gatgcgcggc tggacggctg gacggctcag
     2761 ggaacgacgt ggaacagtgc acgggatttg ctggctgatg aaataatgca gaccatcaac
     2821 ccacaaaaac aacccgacgc cgctcaggcc atcgcgcaga aagtccgctg gaagcatctg
     2881 gccgacgcca ccgaaacgtt gcgtgccgag cgcatacgga caaaaatccg gccgtcccgg
     2941 aaagggcaaa accagcaaac gcaaacaaaa ctcttcgccg acgtaagcgt ggactttttt
     3001 aacgaatatc ctacgccagc ccgcggtgcg atgcatgttc tgcggctgcc gttcggttgg
     3061 aagcgcgtac aggatgaagt gtcgtataat attgcgcctg gcggcagaaa aacgctggcg
     3121 ataacaatag aaggtgaaaa gctgccttat atcgccaacg gcaaaatacc cgtcgacatc
     3181 cgtgtgaata ccgaccaggc tgagccgcaa accctgctga ccgaagtgcc catagtcgta
     3241 gccggcgatt tcaagtctgc cccaaccatc gacggcaatc ttgcagattg gcctgaagca
     3301 accaataccg caggcgattt ccgcctgctg ggccgtcgcg gcgaaaccgg cagcggcctt
     3361 gccgaacgac aaaccaccgt ctacaccatg caggacgatg acaatatcta tttcgcgttt
     3421 tactgcaagg aggcgtcgcc gcagtctctg cgtgccaacg cagccaacac aatacgttat
     3481 gacaaactgc tggcatgcgg cgaagattat gtggaagtca tactcgcacc gggcggcgaa
     3541 gcgaaaaaac cgcaggatct ttatcacata atggtcaaac ccaacggcgt agtcatacag
     3601 gagcacggcg taggctgtac gccgccgctg ggcaaggcgc agcgggaaga tttcggaaca
     3661 gtcgcagctg taagcagagg cgatgacttc tggattgcgg aaatcaaagt tccccgcaaa
     3721 aatttcggcg aacacggcga cgaaccgttc tggggcgtaa attttgccag atttgcaacc
     3781 tcaacacagg aagcatccag ctgggccggc gctgaaagat atttttacga cccgcaatca
     3841 ctgggtacgc tcatactcaa aaaaacagag aattgattat cgcgaacaat tacgtaaaat
     3901 gacgacaggt tacatgacaa ttttttttaa aaaaacacgg agattttaaa tggcgacgaa
     3961 gataagctgg ctggtcggca tattactgat agtgggtttt ctgggttttc ttggaatgcg
     4021 gctgttttct cccaggcccg atgcgcctac cactgcaact accaaagccg gcgttctgga
     4081 aatcatcaaa ccagcaatgc cgatatcgga aattctcggc tcgcaaccat ccggcaaaaa
     4141 caatgccggc gaatattata atgatgccgt actttatttc agagaaaaca aaaaggattt
     4201 taaggaaatt tacaatcaga tgacagccgg cacggaaaat ccacagatgt tcggctggca
     4261 ggaagacctc tgcctcaacc ttcagaaaaa actcgcacca gccgccgaat gcgagacaat
     4321 gaaatatacc ctgctcttta cgcctgataa attgaccgtt tcgggcaagc cggacggcca
     4381 ggatgatctt tactggctga aaaacatgct ggaactgcta tgcatgagct atcagcatga
     4441 cagccagtgg caaaaagccg tcgaatccgc caagaccatg ctcctgctcg gatggcatat
     4501 gacgcaggat gattcccgcg cgatgtatca gataaccggc cttgacatac aggctgacgc
     4561 ggcacagcgg ctggcgtttt attacggcaa actgggcaat acgaaaatgt ccaaactctg
     4621 cgacgactac tacaatcaag ccatcactgc cggcactctg gcacacgaga aatataacaa
     4681 tgtgtggaat cttcggcagc ggcccggcgg caaacagggg ccgttccctg gcgacctgtt
     4741 caacataata gagaacgacc agtcgcgcgg ctggaaggtt gacgcactgc tggcgatgtc
     4801 catcgcaaaa ttgcaggaag ccaaaaccaa aggcaatcac gccgaaatag aacgtctata
     4861 tgacaaatat tcgcaatgca acgatccgct gttgcgcgcg gcggcgaagg cgtcgcagac
     4921 agctacaaaa gacgacatcg aaaacgccat gcgcggcgac agcgaataac agtctgaatc
     4981 aaaatgaaaa cttatccggc aatgttgaat ctttccggac ggctggtagc cgtcataggc
     5041 gcaggaacta caggactgcg aaaagttcgt tctctctgcg atgccggagc aaaagtacga
     5101 ctgattgccg gaaaaaattt caatccgggt tgcgaactgc cagacgatgt ggaagtgctt
     5161 gcggaagatt ttgattccaa aacgtcaacg agacttttgg aagattgccg gctgatattc
     5221 gcatgctgca atgaccggaa aaccaacagt gaaatagcgg cggtcagccg caagctgggc
     5281 atatgggtaa acgtcgcgga tcagccggaa gattgcgact ttttctgccc ggcggtactc
     5341 tgtaacggcg acgtcaccat agcgataggc acaggcggca acgcgccggg cctgtcggcg
     5401 gaactgaaaa aattcatctc ccgcaatctg ccggctgaca caggcagatt cgcggaatcg
     5461 ctcagtcatc tgcgggctga attgaaaaat tcatgtcacg acccgtccgc aagacaggct
     5521 gcgatgaaaa aattatcatc ggaagaaggc tacgccgcct tcgcctcacg gggcgaacac
     5581 ggcttgcgtg aaatgctatc cggtttgcaa ggcggtaatt gaaaatgctg gttatctgtt
     5641 tcggcgtaaa tcacaaaacc gcggaacttt cgcttcgcga aaaattcgca ctgagcccga
     5701 ctcagcttga caataaactg cgcgaacttt ttgaaaaatt tccggctgcc gagtttctgc
     5761 tgctcagcac gtgcaatcgt accgaactat acattatccg gtcgctgcac ggccatcccc
     5821 gcaacgagga ggtaactgag tttttttccg caatgaccgg cctggaacag tcagccttcg
     5881 ccgaatcact gtacgttttg caggactccg aggcggtggc acagatgttc gcagtagccg
     5941 ccggcctgga cagcatggta ccgggtgaaa gtcagatagt ttcacaactc aagcaggcgt
     6001 tagccgcggc acaacggctg caggtcgcca gggcgaacat gacgacgctg gtggcggaag
     6061 cgctacacgt cgccaaacac gtccgctcgg aaaccggcat agcacagggc aaagtgtcgg
     6121 tggcgtcggt ggcgttggaa ctgatagcca accgtttcaa aacgctggcc ggcatgacgg
     6181 tactcagcgt cggcgcaggc aaaatgaacg aaatcatact gcaaactctg cgaacaatgc
     6241 tgccgagcga actgctcata accaaccgct cgccggaaaa agccacggaa ctggctgaat
     6301 cgtgcggcgg aaaaatcgta ccgtttgaag cgctcgcgga acttgtaggc cgcagcgaca
     6361 taataataac gtccactgca tcgcagtcgc cgattataac atgcgacatg ctcgcatccc
     6421 tgccgccgaa ggaacggctg ttcgtggaca tggctgtgcc gcgagacata caggctgatg
     6481 cggaacttct ggacggcgtt gaactttata acatcgacga cctcaatgga acggtcagcc
     6541 agactctcga cgaacgattc aagcacgtag agcaggcgga aaa
//
LOCUS       DAONFI010000098         6537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13826_length_6537_cov_3.599815, whole genome shotgun sequence.
ACCESSION   DAONFI010000098 DAONFI010000000
VERSION     DAONFI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13826_length_6537_cov_3.599815"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLK08_04370"
     CDS             complement(<1..1129)
                     /locus_tag="PLK08_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPS52770.1"
                     /translation="MGNNQRRVAEEVSMHAGLGKLEMNHFPVVGTTYTASANNRITDS
                     AAAVTAIACGVKTNNGMLGVSPDEKPLVSIAADARNAGWKVGIITSVPLNHATPAGFY
                     AHVVSRKMYDDIAMQMAASGFDFFGGAGVIWKGGGDIYAILAQNGYATAIGLDALRRL
                     AKTPAVAVEHVGDGQMSPRYAHNNAAYKSTADVMTLADLTANAVRLLDNPVGFFIMVE
                     GGAIDWACHDNDLPDCIWETIAMNDAVRVAMDFARKHPNETLIITTADHETGGLTVQW
                     PIDVQKIMKLKSPSEIQKILLDCAKQKTGENLFAMLDEKFGLKNLSDDDKAVIQKFWD
                     SGQIAGKHGVALEVLRLTYANVGISWTPGAHTGKPVTTTAVG"
     gene            1378..1532
                     /gene="ssrS"
                     /locus_tag="PLK08_04375"
     ncRNA           1378..1532
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLK08_04375"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1724..3172)
                     /gene="purB"
                     /locus_tag="PLK08_04380"
     CDS             complement(1724..3172)
                     /gene="purB"
                     /locus_tag="PLK08_04380"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPS52771.1"
                     /translation="MTDTNDMNRHDTYASPLESRYASEEMRKIFSDRMKFTTWRRLWL
                     CLAQCQQELGLAISDEQLSQMAANIDNIDYAAAAEYEKKFRHDVMAHIHAFGDVAPAA
                     AGIIHLGATSQFVNCNTDMIQMREALRLILAHLVTVIDSLAEFAEKWKNQPTLGFTHF
                     QPAQMTTVGKRATLWCYDFVRDLREVEYRLANLEFRGVKGTTGTQASFLALFDGDHEK
                     VRKLDRMVAGRMGFDKIAPVTGQTYSRKVDAVIAQTLAGIGVSVHKFCNDVRLLAHRK
                     EVEEPFGKDQVGSSAMAYKRNPMRCERATGLARFLMDISVSPMHTAAEQWLERTLDDS
                     SNKRLAMAEAFLSCDSALRIVENVSAGLVVYPAVIETNTRAELPFMATENIIMAGVKA
                     GGDRQVLHEVIRKHSMDAGKQVKMYGKPNDLIDRLKADSACADIDIDSLLDPITFVGR
                     APQQVDEFLDEVVTPILRQYDKLIMKTPDLSL"
     gene            complement(3228..4847)
                     /locus_tag="PLK08_04385"
     CDS             complement(3228..4847)
                     /locus_tag="PLK08_04385"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPS52772.1"
                     /translation="MKRFAIFAVLCLSLSCALADEIMFSGNEDGRWIARAYRLDNADM
                     FDIYHTSEGRPFEPLLQQIKGKLAAIQTVGRTLRVIYNERSCAIIGENGTLLTTSNLP
                     GKFMAMCESPADPASQDELMVLVARPDCSRELAAASVQVKPDEWWCLYTSYGLEFKPV
                     PARTGLTETKLGATRASMVQSGGKIYVLLFSSRGASLVTWSAFEDWKKFPPFKETPHD
                     ILSIDDTIFLVYPRKVVNNANLELRMAKLSPEGKVGALMPVVDPLTREALAWDADTKM
                     TVSSLGQVAGICWKDRTKWDFLTVDEKARAGKTMTVDDLLRASSRMQRVEEVQQYFMI
                     AIITAGFVVMFLLKPYVTTPRLLVLPPGLKPANMLKRVVAIIVDLMIVQIPIGIAANY
                     FIINVRNIPRDEIMDMLKDDPQRIVEACPELLYFGMAFVVIFAIYGTAMEMMCGWTVG
                     KRIMGLRVVTDDAIKPPLREAALRNIFKIVALVSISPASDMVWYFILLVIVMFVTRYR
                     QRIGDLVARTIVVEAALPAEQQQPQPPMQDN"
     gene            complement(4853..5833)
                     /locus_tag="PLK08_04390"
     CDS             complement(4853..5833)
                     /locus_tag="PLK08_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52773.1"
                     /translation="MKIAIYSVCLCSAVLMLVGGCAQPKCPGTELLTPMNDLVSDYNA
                     NAAAMPRLWARTRIQISGKYKGVPFNYGSVSPKADPNGLLIIRKNDKDPFGPQDFVLI
                     YREAGNELARLGISRADGVYYSWNKPIDSCWYGRLALAGAQGVNVPLDPVQLPGIVGI
                     VPLPQKGDNPLAVHYVRQEWPFAQVLEYIERQRTAKGCDGHMLLKRETFFRWQIDPAQ
                     PTAILPQEVFMVKLYDNRGVNVMTAYMDNYQPVKLEDADPNQPVVAKLPTDIKLVWQS
                     GECVHMLLSDMTTEDKVDPAVFEFYNRLPAGMGAKTRRVDANVTSEGVTE"
     gene            complement(5830..6366)
                     /locus_tag="PLK08_04395"
     CDS             complement(5830..6366)
                     /locus_tag="PLK08_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52774.1"
                     /translation="MSKEFKIAKSEGLCHKCEQPLAEEAPIVALVRATGDELELRRED
                     YHVECYEAMAAESDPLNDTDVMGVWRTTVPKKEEKKKLLIDDGMLLTFFEQLEGQDDS
                     SRLNFRYVLTLILMRKRMLSYEGMEKLDDGREIWKMRLRGSDKIYNVIDTRMTEDQIA
                     EVSASLGDIMQDDFEEKE"
BASE COUNT         1585 a         1801 c         1504 g         1647 t
ORIGIN      
        1 ggccaacggc ggtggtggtt accggcttgc cggtatgcgc gccgggtgtc caggaaattc
       61 cgacattcgc ataagtcaac cgtaaaactt caagcgctac gccatgttta ccggcaattt
      121 ggccactatc ccaaaatttc tgaataacgg ctttatcgtc gtcggaaaga tttttcaatc
      181 cgaatttttc gtcgagcatt gcgaacaggt tttcgcccgt tttctgtttc gcgcagtcga
      241 gtaaaatctt ctggatttca gacggcgatt tcagcttcat tattttttgt acatctatcg
      301 gccattgtac ggtcaagccg ccggtttcat ggtcagccgt cgtgataatc agcgtctcat
      361 tcggatgctt tcgggcaaaa tccatcgcca ctctaactgc gtcattcatg gcgatggttt
      421 cccatatgca gtccggcagg tcattatcgt ggcaggccca gtcgatagcc ccgccctcga
      481 ccatgatgaa aaatcccaca ggattatcca acagccgcac ggcatttgca gtcaaatccg
      541 ccagagtcat cacgtcggct gttgatttat atgcggcatt attatgtgca taccgcggcg
      601 acatctggcc gtcaccaaca tgctcgacgg caacagcggg cgtcttcgca agcctgcgca
      661 aggcatccag cccgatagcc gtcgcataac cgttttgggc gagtatcgca tatatgtccc
      721 caccgccctt ccatattacg cctgctccgc caaaaaagtc aaacccgctg gcagccatct
      781 gcatggcgat atcatcgtac attttgcgcg acaccacgtg agcataaaaa ccggctggtg
      841 tggcgtgatt caacggcacg gacgttatta ttcccacttt ccagccggcg tttctggcat
      901 ctgcggcgat gctgaccagc ggcttttcgt caggcgatac gccaagcatg ccgttattcg
      961 tcttgacgcc gcaagcaata gccgtcactg cggcagccga gtcggtaatc ctgttattcg
     1021 ccgatgccgt atatgtagtt ccgacaaccg gaaaatgatt catctccaat ttgccgaggc
     1081 cggcatgcat ggaaacttct tccgccaccc tgcgctgatt gttccccata ccatcgccga
     1141 tgaaaaggaa aatgtatcgg ggcgattgcc cgaatatata cgatgaaaat agaaccagaa
     1201 ataagattac gccgccaaat tttttttgca tattgcatct cctgaacagg ccggtacata
     1261 ccggcatttt ttattatatt gcgaaaattt cgtttttaaa gtgcgttata acgaattgtc
     1321 gaaatcttaa aatcaccttg taaaatcagg gcacaagtgc tattataatt agtgaagaga
     1381 cctctacggt gttcgacaat cggatttatt tgaaaagaac cgatgatatc caaaggggag
     1441 gccaagtcca agtcggctga gaccggcggc agccacccac cttaaacttt gggttccttt
     1501 caactaatcc ggacgacaca cgcggaggtt tttttatgtg caactttccc accgtttgag
     1561 ttttagagcg gctttcaaaa ttattttcct tcgcaaaccg ccacattcta aacatcatga
     1621 atttgtggcg gtaatatttt aaaaatcggc ttaaaaacca actaattacg gcagtaatat
     1681 ttaaaaaatc ggcttaaaaa ccaaataatt acgacggtga tatttaaagg ctcaaatccg
     1741 gcgttttcat aataagtttg tcatactgcc gcaggattgg cgtcacaact tcatccagaa
     1801 attcgtccac ctgctgcggt gccctgccga cgaatgtaat cgggtcaagc agcgaatcta
     1861 tatcgatatc ggcacaggcg gaatcagcct tgagcctatc gataagatcg tttggtttgc
     1921 cgtacatttt gacctgtttg ccggcatcca tggaatgttt acgaataacc tcgtgcagca
     1981 cttgccggtc gccgccggct ttgacgccgg ccattatgat gttctcggta gccatgaacg
     2041 gcagttccgc gcgggtgttg gtttcgatta cagcaggata aacgacaagg ccggcagaga
     2101 cgttttccac tatccgcaac gccgaatcgc acgacaggaa cgcctcagcc atcgccagcc
     2161 gtttattcga cgaatcgtcc agcgtccgct caagccactg ttccgcagct gtatgcatcg
     2221 gacttacgga tatgtccatc aaaaatctgg ctaagccggt tgcccgctca catcgcatcg
     2281 gattgcgctt gtaagccatc gccgatgagc cgacctggtc tttcccgaac ggctcttcca
     2341 cttccttgcg atgtgcgagc agccgaacgt cattacagaa tttatgtacg cttacgccaa
     2401 tgccggctaa cgtctgggct atcacggcat caaccttgcg agagtatgtt tggccggtaa
     2461 ccggcgcaat cttgtcaaat cccatacggc cggcaaccat tctgtcgagt ttgcgtacct
     2521 tttcatggtc gccgtcaaat aacgcaagaa aactcgcctg cgtaccggtc gtacccttca
     2581 cgccgcgaaa ctccagattc gccagccgat attcaacctc ccgcaaatcc cgcacgaaat
     2641 cgtaacacca caacgtcgca cgtttgccga cggttgtcat ctgggccggc tgaaaatgcg
     2701 taaaacccaa cgtgggctga tttttccatt tttccgcaaa ttccgccaac gagtctatga
     2761 ccgtcaccag atgcgcaagt atcagtcgca atgcctcgcg catctgtatc atgtcggtgt
     2821 tgcagttgac gaactggctg gttgcgccga ggtgaattat gcccgccgcc gccggcgcaa
     2881 catcgccaaa ggcatgaata tgagccatca cgtcgtgccg gaatttcttt tcatattcgg
     2941 cagcggcagc gtaatcgata ttatcaatgt tggcggccat ctgactgagc tgctcgtcgg
     3001 aaatggctag gcccagttcc tgctggcatt gcgccaggca cagccatagc cgccgccacg
     3061 tggtaaattt catcctgtcg gaaaaaattt tacgcatctc ttcactggca tagcggcttt
     3121 cgagcggact cgcatacgta tcgtgcctgt tcatatcatt cgtgtcggtc atgataagat
     3181 tcctgaaatt caatttctga caaaattaac gaaggttaaa aaatcaatca attatcctgc
     3241 attggcggtt gcggctgttg ctgttcagca ggcagagcgg cttcgacgac aatggttcgg
     3301 gctacaaggt ctccgatacg ctgccgatac cgcgttacga acatgacgat taccagcaga
     3361 ataaaatacc acaccatatc cgaagccggc gaaatgctga ccaacgcgac gattttaaaa
     3421 atattacgca gagccgcttc acgcaacggc ggctttatgg cgtcatctgt cactacgcgc
     3481 aaacccatga ttcgcttgcc gaccgtccag ccgcacatca tctccatagc cgtcccgtaa
     3541 atggcgaata tcaccacgaa cgccatgccg aaatataaca gctccgggca ggcttcgaca
     3601 atccgctgcg gatcatcctt gagcatatcc atgatttcat cgcgcggtat gttcctgacg
     3661 tttatgataa aataattcgc ggcaatgccg attggaatct gcactatcat caggtcgacg
     3721 ataatcgcaa cgacccgctt gagcatgttt gccggtttaa ggccaggcgg aagaacaagc
     3781 aagcggggcg tcgtaacata cggcttgagc agaaacatta cgacgaaacc ggcggttatg
     3841 atggctatca tgaaatactg ctggacttct tcgacccgct gcatgcggct gcttgcgcga
     3901 aggaggtcat cgacggtcat tgttttgccg gcacgcgctt tttcgtcaac cgtaagaaaa
     3961 tcccacttcg ttctgtcttt ccaacatata ccagccacct ggccgagcga cgaaacagtc
     4021 attttcgtat ccgcatccca cgcgagagcc tcccttgtca ggggatcgac taccggcatt
     4081 aacgcaccga ccttgccttc cggcgatagc ttcgccattc gcagttccag gttagcatta
     4141 tttacaactt tccgcgggta gactaaaaaa attgtgtcgt caatcgaaag aatgtcatgc
     4201 ggagtttcct tgaacggcgg aaattttttc cagtcctcga acgccgacca tgtaaccaga
     4261 gacgctcctc tggaagaaaa taaaagaacg taaattttcc cgcccgactg caccattgaa
     4321 gctcttgtcg cgccaagttt cgtttccgtc agacctgttc tggctggaac gggtttgaat
     4381 tccaacccgt aagacgtgta aagacaccac cattcgtcgg gtttgacctg aacactggcg
     4441 gcagccagtt cacgcgagca gtcaggcctg gcgacaagaa ccataagttc atcctggctg
     4501 gccgggtcag ccggcgattc acacatagcc ataaatttac caggcagatt gctcgtagtc
     4561 agcagagtgc cattttctcc gataatagca catgagcgtt cgttatatat gacacgaagc
     4621 gtcctgccga cggtttgaat agcagccagt ttgcctttaa tctgttgaag cagcggctca
     4681 aacggccggc cttccgaagt atgataaatg tcaaacatgt cggcgttatc cagccgatac
     4741 gcgcgcgcta tccagcggcc gtcttcattg ccggaaaaca ttatctcatc ggctaacgca
     4801 caggagagcg aaagacacag aaccgcaaat atagcaaatc gtttcaaaaa cactattcgg
     4861 taactccttc tgacgtcacg ttcgcatcca cgcgacgcgt ttttgcaccc atgccggcag
     4921 gcagacgatt ataaaattca aacaccgccg gatcgacctt atcttccgtt gtcatgtcgg
     4981 acagaagcat atgcacacac tcgccgctct gccagacaag ttttatatcg gtaggcaact
     5041 ttgcgacaac cggctgattc gggtcggcgt cttcaagttt taccggctga tagttatcca
     5101 tgtatgccgt catgacattc acaccgcgat tgtcgtacag tttgaccata aaaacttcct
     5161 gcggcagtat ggcagtgggc tgtgccggat caatctgcca gcggaagaat gtttcacgtt
     5221 tcagcagcat gtgcccgtca cagcctttcg ccgtacgttg acgttcaata tattcaagca
     5281 cctgtgcaaa gggccattcc tggcgaacgt aatgtacggc aaggggattg tcgcctttct
     5341 gcggcagcgg tactatgccg acgatgcccg gcagttgtac cggatcgagc ggcacattta
     5401 cgccctgtgc gcctgctaac gccagccggc cataccagca actgtctatc ggtttgttcc
     5461 acgaataata aacgccgtcc gccctgctta tgcccaggcg tgccagttca ttaccggctt
     5521 cgcgatatat cagcacgaaa tcctgcgggc cgaaggggtc tttgtcgttt ttccgtatta
     5581 tgagaaggcc attcgggtca gccttcggcg aaactgaacc gtaattgaac ggcacaccct
     5641 tgtatttgcc ggatatctgt attctggtac gcgcccacaa ccgcggcatg gcggcagcat
     5701 tggcgttgta atcactcaca aggtcattca tgggcgtcag cagttccgtg ccgggacact
     5761 tcggctgagc acagccgccc accagcatca gtaccgccga acaaaggcaa actgaatata
     5821 tcgctatttt cattcctttt cctcgaaatc gtcctgcatt atgtcgccaa gcgaggcgct
     5881 cacttcggct atctggtctt cggtcatcct ggtatctatg acgttgtaaa tcttatcact
     5941 gccgcgaaga cgcatcttcc aaatctccct gccgtcgtca agtttctcca tgccttcgta
     6001 actgagcatc cttttccgca tgagaatcag cgtcaggacg tatcggaaat tcaggcggct
     6061 ggaatcatcc tggccttcaa gctgctcgaa aaacgtcaac agcatgccgt cgtctataag
     6121 cagttttttc ttttcttcct tttttgggac tgtcgtgcgc cagacgccca taacgtccgt
     6181 atcattcagc gggtcagact ctgcagccat cgcctcgtaa cattcgacat gataatcctc
     6241 acgccgaagt tccagttcat cgccagtcgc cctgacgaga gccactatcg gcgcctcttc
     6301 cgccagcggc tgttcgcatt tgtgacagag gccttcactt tttgcaattt taaattcttt
     6361 agacatcttt tttaatcatc aaatttacaa ttttaaattc tttaggcatt cttttttaat
     6421 catcaaatcc gaattcaata ccgtaatcgc ttcataaaat aacagaaatc aggaaactcg
     6481 tcactagaac ctatcccaaa aaccttggct gcctgcaatc ggctcttttg cagcctg
//
LOCUS       DAONFI010000099         6504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13930_length_6504_cov_4.084199, whole genome shotgun sequence.
ACCESSION   DAONFI010000099 DAONFI010000000
VERSION     DAONFI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13930_length_6504_cov_4.084199"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1681
                     /locus_tag="PLK08_04400"
     CDS             305..1681
                     /locus_tag="PLK08_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS52775.1"
                     /translation="MKLLLINPFNPLINLADVKSSRWNRYRIWKPLGLLVLSALTPKE
                     WDVTIIDENIGRVDYNKMDTPDFVGISAFTSQAPRAYELAAEFRAKKIPVVMGGIHAT
                     MCTQEAAKFVDSIVTGEAEIVWPQVLQDVIAGRLQSLYHGGHIDMAHMPIARHDLLTK
                     GYAFGSIQTTRGCPLNCKFCSVTAFNGNKYRNRPIENVIEEFRAIPEKRVLIVDDNLI
                     GITPAHIERAKELFRAMIAANIRKKWMAQVTINFADDEELLTLAEKAGCSGVFIGFES
                     SSATGLAEVGKKFNMLKSRNFHHSIRHIHKHKILVVGSFIMGLDADKPGIGKQIAAAG
                     KSYGVDILNVLFLTPLPGTHLWNDMCGQDRILLCDFPADWKYYTLGFPTAKYNNFSFR
                     EILHEMKLCDGSFYSRKQILLRAFKNFIYRHQPFVSLISNLSFRHNAKMAQKGYLKLL
                     RSRTSLVT"
     gene            complement(1764..2684)
                     /locus_tag="PLK08_04405"
     CDS             complement(1764..2684)
                     /locus_tag="PLK08_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPS52776.1"
                     /translation="MFMWIIILLAAAIMLLLAIVFSGILGWANEALAVEVDPKIEAIG
                     AALPGANCGGCGFVGCGDYAEAVVIRGVAVNLCPVGGDAVARKIAQILGVELKETYPY
                     RPAIHCGAKCEDKLGRHEYIGEHSCTAANLVAGVQGCAYGCLAMGDCERACKFDAIHV
                     INGQTVVDYDKCVGCGACEKACPRHVISMVPFKAEQMYILRCNNKDFGKDVKSVCKVG
                     CIGCGACARQATNLIKMQGKLPQFDYDNYDPATIHQIMQTVIEKCPVGGYVKIGKPSR
                     EDVAKVSDEKMPDVVVGTFETTVDKTDWRG"
     gene            complement(2687..3448)
                     /locus_tag="PLK08_04410"
     CDS             complement(2687..3448)
                     /locus_tag="PLK08_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPS52777.1"
                     /translation="MEYLSQIILIAVGAAIVNNFVLSYFVGICPFIGVSRRVDMAFGM
                     GCAVTFVISVAGFLSWVFTYYLLRSGAPLSTWIYHLCGGTGDSDLSILNYIVYIFLIA
                     ASVQLVEMYLRKFFPPLYKSFGVFLPLITTNCAILFACLEIMKHISGDNPWDLGQSLT
                     FAFFGGVGFTLALLIMAGIREDLELCNVPKSLRGAGITLVIAGILAMAFMGFNGVNAS
                     LQKALVVKPAHATQTAVMGDVEQMVKKTNLAEETK"
     gene            complement(3458..4093)
                     /gene="rsxE"
                     /locus_tag="PLK08_04415"
     CDS             complement(3458..4093)
                     /gene="rsxE"
                     /locus_tag="PLK08_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HPS52778.1"
                     /translation="MPDNNGPTAFERFINGILPENPTYRQVLGMCPTLAVTATMKASM
                     TMAGAVAFVLICSNIIVSLIRHLIKPHLRILVFTLIIATFVTIADKFLAAFMPDMSDQ
                     LGPYIPLIIVNCIIICRCEVCAAKQSVGPAIADAVGQSIGFALGLSTVATVRELLASG
                     SWFGIELFWNGSATEGYRWAIMGLAPGAFLTLGLLMGLANWLSAKFEKKAA"
     gene            complement(4096..4761)
                     /locus_tag="PLK08_04420"
     CDS             complement(4096..4761)
                     /locus_tag="PLK08_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPS52779.1"
                     /translation="MLKYFKQGWLVIAISLAFGALLAGVQLTLGPKIKENQITKSYNR
                     IPLLLLGQDKVNGAKIVIAGDKITVDRDGKAETTLTVQKPENPLDYDVYKVFNGDKQL
                     GWVVLGKGNGYADEVVCLIGLSPDLKTITGVSVISQKETPNLGSKIQSPWIYQFAGKA
                     ANPPLEVVKNRTASPDNNKIDAISGATISSDSLTAIVNQTVAKFVGDVEKLKFASAAA
                     EEK"
     gene            complement(4902..5024)
                     /locus_tag="PLK08_04425"
     CDS             complement(4902..5024)
                     /locus_tag="PLK08_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52780.1"
                     /translation="MKLLNVPAELQDMRHRLIDETTELLHILSAIINKQNRSGS"
     gene            complement(5021..5278)
                     /locus_tag="PLK08_04430"
     CDS             complement(5021..5278)
                     /locus_tag="PLK08_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPS52781.1"
                     /translation="MNDSNNNMLKTYDFEDRTLEYAITVRTFLRHLPKTISNHEDGKQ
                     LIRASGSVGANYIESKESLGKRDRFYGLKLPEKKQKKVVIG"
     gene            complement(5313..6389)
                     /locus_tag="PLK08_04435"
     CDS             complement(5313..6389)
                     /locus_tag="PLK08_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPS52782.1"
                     /translation="MTEENKSNQADIEAAVVVMPSPHFKDKKSSTRRMMIDVLIGLSP
                     LMLVALIIYRQFAVRQVVVCVAACMLAELVFMKMRKLPLTLSDCSAAVTGVILAFSIP
                     WNTPCYVAIIGCVVAIGIGKVIFGGVGMNLFNPAMVGRAFIMLAFAGALGAPGYVKDL
                     DKMPWLASLNATPSQYAAIVPAGTMPDAVSAATPMTAAKDYQTSGGKKGFIPKLSNLF
                     WGNHIGSLGEISAIACILGGAYLLIRRTASWQVPLGVILAVIIISGLRDILNPSDPWN
                     VMHSLCGGALLFGAFFIATDPVTNPMTAKGKFIFGLGVGGCIELIRIMSGYPEGVMFA
                     ILLMNAVVPLINRWTVPKPFGTSK"
BASE COUNT         1639 a         1750 c         1516 g         1599 t
ORIGIN      
        1 ttcgtgttct gctggttatt ttgacttcaa ctatgcaggc cggactgccg gcgtaaccaa
       61 atgatataca gccccggtaa tatccagaca ttaattcgat aagatatatg gacattatgc
      121 aacaggttta actgtagcca tttatcaata tcgcaccttt tttgattcaa ttcacctctt
      181 tatttgaaaa taccgctgtg gggtaaatcc tgaaaatacc aagatacaac ctgacttata
      241 atattttgaa atatttaaac taatcatctt aaaaaatgat ataatgaata attaaggaaa
      301 tcatatgaaa ctgctcctga ttaatccgtt caatcctctt ataaatctcg cagacgtcaa
      361 atccagccgc tggaatcgct acagaatctg gaagccgctg ggcctgcttg tgctctctgc
      421 ccttacgccc aaagagtggg acgttacgat tattgatgaa aacatcggca gggtcgatta
      481 caacaaaatg gacacacccg atttcgtcgg catatccgca ttcacgtcac aggcgccgcg
      541 ggcatatgaa ctggccgccg aatttcgcgc aaaaaaaatc cccgtcgtca tgggcggaat
      601 acacgcaacc atgtgcacgc aagaagccgc aaaattcgtg gacagcatcg tcaccggtga
      661 agctgaaata gtttggcctc aagttctgca ggatgtaatt gcggggcggc tgcaatcact
      721 ataccatggc ggacacattg atatggccca catgccaatc gcacgccatg acctgctgac
      781 aaaaggctac gcgttcggtt ccattcaaac cacgcgaggt tgcccgctaa actgtaagtt
      841 ctgcagcgtt actgcgttca atggcaataa gtatcgcaac agacctatcg aaaatgtaat
      901 cgaagaattt cgggcgattc cggaaaaacg tgtactaata gtggacgaca atctcatcgg
      961 cataactccg gcacacattg aacgggcgaa agaactgttc cgcgccatga ttgccgccaa
     1021 catccgcaaa aaatggatgg cacaggttac gattaatttt gcagatgatg aggaactgct
     1081 cacactggcg gagaaagccg gctgcagcgg agtattcata ggctttgaat catcatcggc
     1141 aacaggattg gctgaagtcg gcaaaaaatt caacatgctc aaaagccgta attttcacca
     1201 ctccatacga cacattcaca aacataaaat tctagtagtc ggttcattca tcatgggcct
     1261 tgacgcagac aagcccggca taggcaaaca aatagcagcc gccggcaaaa gctacggcgt
     1321 ggatattcta aacgtgctat tcctcacgcc gctgcccggc acgcacctgt ggaacgacat
     1381 gtgcggacag gacaggattc tgctgtgcga ctttccggct gactggaaat attacactct
     1441 gggctttccg accgcgaaat acaacaattt ttcatttcga gaaattttgc atgagatgaa
     1501 actatgcgac ggctcgttct attcgcgtaa acagattctg ttgcgggctt tcaaaaattt
     1561 catctaccgc catcagccgt tcgtatcgct gatatcaaac ctttcgtttc gccacaacgc
     1621 caagatggcc caaaaaggct atctcaaact gctgcgttca cgcacatctc tcgtgacgtg
     1681 ataactctga aacaccagta acacgaataa aaaagcgaca gaagaaattc cgccgctttt
     1741 tataattcac tcgatgtaaa aaatcagccg cgccagtcgg ttttgtccac agttgtttca
     1801 aacgtgccga cgactacgtc gggcattttt tcatcactga cttttgcgac gtcttccctg
     1861 gacggcttgc cgattttgac atatccgccg actggacatt tttcgattac ggtctgcata
     1921 atctggtgaa tggtggccgg gtcgtagttg tcgtaatcga actgcgggag tttgccctgc
     1981 atcttgatga ggttagtcgc ctgtcgcgca catgcgccgc agccgataca gccgaccttg
     2041 cagacgcttt tgacgtcttt gccgaaatcc ttgttgttgc atcggaggat gtacatctgt
     2101 tcagccttga acggcaccat ggatatcacg tgccgcgggc aggctttctc gcacgcgccg
     2161 cagccgacgc atttgtcata atccaccacg gtctggccgt tgatgacgtg aatggcgtca
     2221 aacttgcacg cccgctcgca atcacccatg gccagacagc cgtacgcgca accctgaacg
     2281 ccggcaacca gattggctgc ggtgcaggaa tgttcgccga tgtattcatg gcggcccagc
     2341 ttgtcttcgc attttgcgcc gcaatgaatc gccggccgat aaggatatgt ctctttgagt
     2401 tccacgccga gtatttgggc tattttccgt gcgacggcat cgccgccgac ggggcagaga
     2461 tttaccgcaa ctccgcggat gacgacggct tcggcgtaat caccgcagcc gacgaaaccg
     2521 caaccgccgc agttcgcgcc gggcagcgct gcgccgattg cctctatttt tgggtctact
     2581 tcaacggcta acgcctcatt ggcccagccg agtatgccgg aaaaaactat cgccaacaac
     2641 aacattatgg ccgcggcaag caatatgatt atccacatga acatgattat ttcgtctcct
     2701 cggccagatt agttttttta accatttgtt caacgtcgcc cattacggcc gtctgcgtcg
     2761 catgagcggg ctttaccacc agcgccttct gaagcgacgc gtttacgcca ttaaacccca
     2821 taaacgccat cgcaagaatg ccggctatca caagcgtaat accggctccg cggagcgact
     2881 tgggaacatt gcagagttcg aggtcttcgc gtatgccggc cattatcagc agcgccagcg
     2941 taaacccgac gccgccgaag aatgcaaacg tcagcgactg gcccaaatcc cacggattat
     3001 cgccggaaat atgcttcatt atctcaaggc aggcgaacag gatggcgcaa ttcgtcgtta
     3061 tcagcggcag aaacacgccg aaactcttgt acagcggcgg aaagaacttg cgaaggtaca
     3121 tttccacaag ctgcacgctg gcggctatga ggaaaatgta gacgatatag ttcagtatgc
     3181 tcaggtcgct gtcgcccgtg ccgccgcaga gatggtatat ccacgtcgag agcggcgctc
     3241 cgctgcggag cagataatac gtaaaaaccc agctcagaaa gccggctacc gaaattacga
     3301 acgtaaccgc acagcccatg ccgaaagcca tgtccacacg acgcgacacg ccgatgaacg
     3361 gacatatccc gacaaaataa ctcaacacga agttgttcac tatcgcggcg ccgacggcta
     3421 tcaggattat ttgtgagagg tattccatgt ttccacctta cgcggctttc ttttcaaatt
     3481 ttgcacttag ccagttagcc agacccatca gcaatcccag cgttaaaaac gctccaggag
     3541 ccaggcccat aatcgcccaa cgatatcctt ccgtcgccga gccgttccag aacagttcta
     3601 tcccgaacca cgacccgcta gccagcaatt cgcgcactgt cgccacggtc gaaagaccca
     3661 gggcgaaacc tatgctctgg ccgaccgcgt cggctatcgc cggcccgacg ctctgcttgg
     3721 cggcacagac ttcacagcga cagatgatta tgcagttgac gatgattagc ggaatgtacg
     3781 gcccaagctg gtcggacatg tccggcatga acgcggcgag aaatttgtcg gcgattgtca
     3841 cgaacgtcgc aataatcagc gtgaaaacaa gaatccgcag gtgcggcttg atgaggtgac
     3901 gtatcaggct gacgattatg ttcgagcata tcagcacgaa cgcaaccgcg ccagccatcg
     3961 tcattgacgc cttcatcgtc gcggtcaccg ccagcgtcgg gcacataccc agaacctgac
     4021 gataggtcgg gttttccggc aatatgccgt ttatgaatcg ttcaaaggct gtaggtccgt
     4081 tattgtcagg catggttatt tctcctccgc ggcggctgac gcgaatttca gtttctctac
     4141 gtcaccgacg aatttcgcga ctgtctgatt gactatggcc gtcaacgaat ccgatgaaat
     4201 cgttgcgccg cttatggcgt ctattttgtt gttatccggc gaggcggttc tgtttttgac
     4261 gacctccagc ggcggattgg cggctttgcc tgcgaactga taaatccacg ggctttgaat
     4321 tttgctgccg agattgggcg tttctttctg cgatatcacc gacacgccgg tgattgtttt
     4381 gaggtcgggg ctcaaaccta tgagacagac aacttcgtcg gcgtatccgt tacccttgcc
     4441 cagcacgacc caaccgagct gtttatcacc gttgaaaacc ttatatacat cgtaatccaa
     4501 aggattttcc ggtttttgca cggtaagagt cgtttcagct tttccgtcgc ggtctacagt
     4561 tattttatcg ccggcgatta ctatttttgc gccattaacc ttatcctgtc ccaacagaag
     4621 cagtggtatg cggttatagg atttggttat ctgattttcc ttgattttcg gccccagcgt
     4681 cagttgcacg ccagccagca gtgcgccgaa cgccagcgat atcgctataa ccagccagcc
     4741 ctgtttaaaa tattttaaca tcaacaatct ccaatattgt aaatatcaag tttcaagtaa
     4801 caacctgcaa gcaagttttt aattaacgcc tcgcggcaaa ccttgtccat aacagccgtt
     4861 tgtgctggct gttaaaatac aaattcctat acactaaatc ttcaacttcc agaacgattc
     4921 tgtttgttaa ttatcgcact tagtatatgt aacagttccg tcgtttcatc tatcaagcga
     4981 tgtcgcatat cttgtaactc agcgggaaca ttaagtagct tcaaccaata acgactttct
     5041 tttgcttctt ttctggcaat tttaagccgt aaaacctatc tcttttacca agagattcct
     5101 ttgattcaat ataattggct cctacagaac cggacgctct aattagttgt tttccatctt
     5161 catggttact tatagtttta ggcaaatgtc tcaaaaacgt tcgaaccgta atagcatatt
     5221 ctaacgtcct gtcttcaaag tcatacgttt taagcatatt gttgttcgaa tcattcattg
     5281 tctattgaaa cttgcttgta atttgtttct tgtcacttgg aagttccgaa gggttttggc
     5341 actgtccaac gattgatgag cggaacgacg gcgttcatta gcaatattgc gaacatcacg
     5401 ccttccggat agccgctcat tatgcgtata agctcaatac agccgccgac tccaaggccg
     5461 aaaatgaact tgccttttgc ggtcatggga ttcgtaacag ggtcggtcgc tatgaaaaac
     5521 gcgccgaaaa gcagtgctcc gccgcagagg ctgtgcataa cattccaggg gtcggacgga
     5581 ttcaatatgt cgcgtaagcc gctgatgatt atcacagcca gtataacgcc cagcggcacc
     5641 tgccacgatg ccgtcctacg tataagcaga taagccccgc ccagaatgca tgcaatcgcc
     5701 gatatctcgc ccagcgagcc gatatgattg ccccagaaga ggttgctcag tttcggtatg
     5761 aacccttttt tgccgcccga cgtctggtaa tccttggctg cggtcatggg cgtagcggcg
     5821 gagaccgcat ccggcatagt gccggcgggc actatcgcgg catattgcga cggcgtggca
     5881 ttcaggcttg ccagccaggg cattttatca aggtctttta cgtaacccgg cgcgccgaga
     5941 gcccctgcga atgccagcat gatgaacgcc ctgccgacca tcgccggatt gaacaggttc
     6001 atgcccacgc cgccgaatat caccttgccg atgcctatcg ccaccacaca gccgataatc
     6061 gcaacgtaac acggcgtatt ccacggaatc gaaaacgcca gaatcacgcc cgtaaccgcg
     6121 gcagagcagt cgctcagggt aagcggcagt ttgcgcatct tcatgaacac caactcggcg
     6181 agcatgcacg ccgccacgca tacaaccacc tggcggactg caaactgacg atagattatc
     6241 aaggctacca gcatcagcgg cgaaaggcct ataagaacgt caatcatcat gcggcgggtc
     6301 gaagattttt tgtccttgaa atgcggactg ggcatcacca ccaccgccgc ttcgatatca
     6361 gcctgattgg atttgttttc ctctgtcata tcatcatctt tccattttta cgagtcgctt
     6421 ctaacaaaaa tcctgttttg aaaatccgga tgtccgtcgc aattattttt cgcgcggcat
     6481 gagggttttg cccattcgta taag
//
LOCUS       DAONFI010000100         6485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_13978_length_6485_cov_4.738725, whole genome shotgun sequence.
ACCESSION   DAONFI010000100 DAONFI010000000
VERSION     DAONFI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13978_length_6485_cov_4.738725"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLK08_04440"
     CDS             <1..1396
                     /locus_tag="PLK08_04440"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPS52783.1"
                     /translation="DPQHPVLVHGGDPTILTYGCEYDHALNRGNDWFYADELDGIGCS
                     SFPKWHGLDDADFGMRVECVKSAARNKKIWLSEVQGGRSSFGFNVYPAVDAASQQRWI
                     WNGLACGADTILFWCWRDEVFGTESGGFGLSGRDGLAQERLDAMCKTGGILKDNATLF
                     EHYKPFAGEVGIFFSPKSYYLCWGQEGNSLRMINAMMGYARSLVRNSIPYRFVEEEHL
                     EELAGLKILFMPRTLVLDAKAEEAIGQFVRNGGTLVCESECGAFNSAGIYRYPEDRFV
                     STLTGISELGRRSLLKESISVDVNGSKLELPANQWLTPYEIGKGKVLAETCDGPAIVE
                     QCVGRGRVVMCGTYFGNGYYGDRREGFESFVSHLVGQSCGEPDIRVTSPTKTKEGFVY
                     VKSGLSRGRRMVFVFFPQGADEVHMEFSRDFFQQDTLVDIFSGNTFRLKNSAAGKSCV
                     LRSSALNLAILIEK"
     gene            1474..>2292
                     /locus_tag="PLK08_04445"
     CDS             1474..>2292
                     /locus_tag="PLK08_04445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPS52784.1"
                     /translation="MDIELTNENYARGMVSSGDSTRTAFFIQKLRDSRPITYAAIGGS
                     ITGGANASSPELSYVSRFGQWLDSKTHCRTINAGIGATTSMFGAYRAHDDILSASPDI
                     ITIEFAVNDTCNPDTAGSFEWLVRQCVDQPQMPLVILIFTMCHDGSNLQNIHIPIGRH
                     YGLPMLSFRDAIYPDIASGQLSWPELSSDDVHPNDDGHRFISEMLIRFLELCDNSSGN
                     LNSDNPVRTLPCPLIKNASRYFYGKVTDAAAMNIISNNGWKQQLHESNRSRYVGW"
     assembly_gap    2293..2302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2303..2584
                     /locus_tag="PLK08_04450"
     CDS             <2303..2584
                     /locus_tag="PLK08_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52785.1"
                     /translation="TIEFEGSIAILGYRKYAGDYGKVFVCVDNKPLTILDGFYEKPAI
                     QKWAGGHTVLEIIYDEPLSAKHRVAIKLLQDTHPDSHGHLFEIGYLLIS"
     gene            complement(2744..3781)
                     /gene="fba"
                     /locus_tag="PLK08_04455"
     CDS             complement(2744..3781)
                     /gene="fba"
                     /locus_tag="PLK08_04455"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015202078.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HPS52786.1"
                     /translation="MPLVPMRILLDHAAENNYGVAALNVNDMEQIQAILMAANETDSP
                     VIIQASRGARQFSNDNYLRHLILAAAELNPNIPIAMHLDHGDTFGTCMSAMAMGFTSV
                     MMDGSLMKNDKGEQVPSGYDYNVNVTRKVVEAAHAIGVSVEGEIGALGGIEDGHGAGL
                     SEEQAQEHLTDPDQAERFVKDTGLDALAVAIGTSHGAYKFKKKPTGDVLKMDLIETIH
                     KRLPNTHLVMHGSSSVPQELQDVINKYGGKLKPTFGVPVEEIQRGIKHGVRKINVDTD
                     NRLAITGAIRKVLVESPEKFDPRDYLKPAREAMKEVCKARMIAFGQAGNAGKIKPITC
                     MEMISFYSKKK"
     gene            complement(4184..6157)
                     /locus_tag="PLK08_04460"
     CDS             complement(4184..6157)
                     /locus_tag="PLK08_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPS52787.1"
                     /translation="MNSWPIYPNFRFATTREYYEILEKNAADFPVVNDELNYEFTGCY
                     TSQSDIKKVNRLSECLLERAEYSAVLADTVLGKKNYPYEELREAWRATLLGHFHDILP
                     GSGVKATREYSSGQFQNTAATAGMIITNSLRAVAKEIDTSFAGVDEPSKDDVQFGAGM
                     GLGGAHCGGISSACHVSGNKRGVVVFNPVACRRNEVISVTVWDAEHDDVHTEQFTACT
                     ADGLKIPAQRLNSGQLWGHRYVDIAVPVSVPAMSYSSLVLESVPDELPLPANAPGYSY
                     LQKTDFVAGVRSLAAERPLAGYHVGTFGMENEFVRVVFDRASGGIVELVDRSTGINVV
                     SPEKPAALLEYVMERPAGMSAWLIREAKKSICPLELTSFAPIQYGPHTAAYEGVYKVG
                     NSTIKVRYSLSAGESQVRIAIQADWMECGGPQIGVPALRLRCPFAVNNAVGTYEIPFG
                     SIKRKPQPAQEVPSQKFVDVTGKCLNGKIGGCAVFNDSKYGYCLDGSTLTCTLIRSSY
                     EPDPLPEQGKFEMKFSLQPHGRELSVAELMHAGAAFNQPLQIVSTDIHVGHMPANGNE
                     AISCMPDSVIITGVKKSEDEDAIIVRLLETQGKDTIAKVKLNHDIFGQFKTAEEIDLL
                     ERSLGNAVLKIMRDGYFSIKMSANSVASVKLKK"
     gene            complement(6154..>6485)
                     /locus_tag="PLK08_04465"
     CDS             complement(6154..>6485)
                     /locus_tag="PLK08_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPS52788.1"
                     /translation="HDFRNPMSSLRLDAQMLEKEAGRGAEARADRMAALAGRMRHTLD
                     RMEKVFQEFLALARPGETAPERLDLGACARECVEMLAPRFEASGVVPDVRLAAGRRGV
                     TCTCARK"
BASE COUNT         1590 a         1645 c         1576 g         1664 t
ORIGIN      
        1 agacccgcaa catccggtac tggtgcatgg cggtgacccg acgattctga catatggctg
       61 cgaatatgat cacgcgctca acaggggtaa cgactggttt tatgcggatg aactcgacgg
      121 catcggttgt tcaagtttcc ccaaatggca cggcttggac gacgccgatt tcggcatgcg
      181 cgtcgaatgc gtcaaatccg cagcccgcaa taaaaagata tggctaagcg aagtacaggg
      241 cggcaggtca tcgttcggct tcaatgttta tccggccgtc gatgccgcat ctcaacagcg
      301 gtggatatgg aacggccttg cctgcggcgc tgatacgatt cttttctggt gctggcggga
      361 tgaggttttc ggcactgaat ccggcggctt cggcctttca ggacgggacg gattggccca
      421 ggagcgactg gatgcaatgt gcaaaaccgg cggcatactg aaagacaatg cgacactgtt
      481 cgagcattat aagccgtttg ccggtgaagt cggcatcttt ttcagtccca agtcatatta
      541 tctctgctgg ggccaggaag gtaattcgct gcggatgatt aacgccatga tgggttatgc
      601 ccgcagcctg gtacgtaatt ccattcccta caggttcgtg gaagaggaac acctggaaga
      661 actcgcagga ttgaagattc tatttatgcc cagaacgctg gttctcgacg ccaaggcaga
      721 agaagcgata ggccaattcg tccgcaacgg cggaacgctg gtctgcgaat cggagtgcgg
      781 cgcattcaat tcagccggca tatatcgtta tcctgaagac cggtttgttt caacgttaac
      841 cggaatatcc gaattgggcc gtcgttcatt gctcaaagag tcaatttcag ttgacgtcaa
      901 tggcagtaag ctggaattac cggcaaatca gtggctgacc ccatatgaaa ttggcaaagg
      961 caaagtgctg gcggagacgt gcgatgggcc ggcgatagtc gagcagtgtg ttggccgcgg
     1021 cagggttgtt atgtgcggta catatttcgg aaacggatac tatggcgaca ggcgggaagg
     1081 tttcgaatct ttcgtgtcgc atctggttgg acagtcgtgc ggggagccgg atatccgagt
     1141 gacatcgccg acaaagacga aagaaggttt tgtctatgta aaatccggcc tctcccgggg
     1201 ccggcgaatg gttttcgtgt tcttcccgca aggagccgac gaagtgcaca tggaattcag
     1261 tcgcgacttt ttccaacagg atactcttgt ggacattttt tccggcaata cgtttcgtct
     1321 aaaaaattcc gcagccggca agtcgtgcgt tttgcgaagt tcggcgttaa acctggctat
     1381 cctgattgaa aaataagccc aatatttttt atgggaatgg tgcgcattgt tataaatgag
     1441 catcgcatgt tttttataca aaggatacat aagttggaca tcgagctgac aaatgaaaac
     1501 tatgctcgcg gaatggtaag ttcaggcgat tccacccgga cagctttttt tattcaaaaa
     1561 ttacgcgatt cacggccaat aacctatgct gccataggcg gctctattac cggaggcgcc
     1621 aatgcttctt cgccggaatt atcatatgtt tcccgctttg gccaatggct cgacagtaag
     1681 actcattgca ggacgataaa tgcaggcata ggcgcaacaa ccagcatgtt cggtgcatat
     1741 cgtgcacacg acgatatatt gtcagcttcg cctgatataa tcaccattga atttgcagtt
     1801 aacgatacct gtaatcctga tactgcagga tcgttcgagt ggcttgtcag gcaatgcgta
     1861 gatcagccgc agatgccgct ggtaattctt attttcacaa tgtgtcacga cggcagtaat
     1921 ttgcaaaata tccatattcc gataggccgt cactacggtt tgcccatgct gtcattccgt
     1981 gatgcaatct atcccgatat tgcgtcggga caattatcct ggccggagct ttcttctgac
     2041 gatgtccatc ctaatgatga tggccacaga ttcatttcgg aaatgctgat aagatttttg
     2101 gaattatgcg ataactcttc aggcaatctc aactccgata atcccgtcag aactctgccg
     2161 tgtccattaa tcaaaaatgc aagcagatat ttttatggca aagtgacaga tgccgccgcg
     2221 atgaatatta tctcaaataa cggatggaaa cagcaactgc atgaatcgaa ccgcagcaga
     2281 tacgtcggat ggnnnnnnnn nnacaatcga atttgaaggc tcaatcgcaa ttctcggcta
     2341 tcggaaatac gccggcgact atggcaaagt cttcgtctgt gtcgacaata agccgcttac
     2401 catcctggat ggtttttatg aaaaaccggc gatacaaaaa tgggccggcg gacatacggt
     2461 tctggaaata atatacgatg aacctctgtc cgcaaaacat agagtagcca taaaactgct
     2521 tcaggatact catcctgaca gccatgggca cttgtttgaa attggttatc ttttaataag
     2581 ctaaagacgt tctcagccac aatacgataa aagtgcacag acacccaagc ttggcaagtc
     2641 cgtaaaattc tgtaaaaaaa ctgcaagcgg caattctttt ttatcggaat caccgcttgc
     2701 agttttatca agtctgatag cgaatcaatt cttcgcgaac acattatttc tttttggaat
     2761 agaaggaaat catttccata caggtaatgg gtttgatttt gcctgcattg ccggcttggc
     2821 cgaaggctat catgcgagcc ttgcagactt ctttcatggc ttcacgggca ggcttgagat
     2881 aatcgcgcgg gtcaaatttt tcaggcgact ctaccaaaac cttgcggatt gcgccggtaa
     2941 tggcaagacg attgtcggta tctacgttaa tcttacgtac gccgtgcttg atgccgcgtt
     3001 gaatttcctc tacaggtacg ccgaaagtcg gcttgagttt gccgccgtat ttgttgatga
     3061 cgtcctgaag ctcctgcgga acgctggatg acccgtgcat gacaagatgc gtgttcggca
     3121 gacgcttgtg aattgtctcg ataagatcca tcttcagtac atcgccggtg ggtttctttt
     3181 taaatttata tgcgccgtgt gaagtgccta tcgccacagc caacgcatcc aggccggtat
     3241 cttttacgaa ccgctccgcc tggtcggggt cggtaagatg ttcctgagcc tgttcttcac
     3301 tcaagccagc gccgtggccg tcttcaatgc cgccgagcgc acctatttca ccctcgacgc
     3361 ttacgccgat agcatgtgcc gcttcaacaa ctttacgggt cacgttcaca ttataatcat
     3421 agccgctggg cacctgttcg cccttatcgt tcttcatcag cgagccgtcc atcatcaccg
     3481 acgtaaagcc catcgccatc gcgctcatac acgtgccgaa cgtatcgccg tggtcgaggt
     3541 gcatggctat cggaatgttc gggttcaact cagccgccgc cagaatcagg tgccgcaaat
     3601 aattgtcgtt gctgaactga cgtgcgccac gggaagcctg aataattaca ggactgtcgg
     3661 tttcgttggc agccatcagg atagcctgaa tctgctccat atcattgacg ttaagggcgg
     3721 ccacgccgta attattctca gcggcatggt cgagcaggat acgcatcggt acaagtggca
     3781 taatatgtcc tttcaaaaaa caggtaattt tgtgtttaca taacctcacc gcattgcata
     3841 ttgcaaacag cgttgcaggg gaggcttatc agcagagata ttatagtaac tcatttgtcg
     3901 attttaaact tttttcccac gctgattcga taatttatta gtaaaccaag ggttatactt
     3961 tatccaaaat aaccgtttta gttataaatt tcggtaaaga tttttaaatt ttttaaaaaa
     4021 ttgcaaaagg ttcacgcaat attctcgcaa aaatcgcagt aatgtatctt cttacaaaag
     4081 aaatcgttac cttctcgact cgtacgcaaa aatttgttaa atcggtcagt ttgaattttt
     4141 ttattactac ctcacataaa accacgggct tacccatgtg tatctacttt tttaatttta
     4201 cgctggcgac tgaatttgcc gacattttta tcgaaaaata accgtcgcgc atgattttca
     4261 acactgcatt cccaaggctg cgttccagca aatcaatttc ttccgccgtc ttaaactgtc
     4321 caaaaatatc gtggttcagc tttactttcg caatagtgtc cttgccctgc gtttccagaa
     4381 gtcgcacgat aatcgcatct tcatcctctg atttctttac acccgtgatt attacactgt
     4441 caggcataca cgatatcgcc tcatttccgt ttgccggcat atggccgaca tgtatatcgg
     4501 tggacactat ctgcagcggc tgattgaatg ctgcaccagc atgcatgagt tcggcaacgg
     4561 acaactctct gccgtgcggc tggagtgaaa atttcatttc gaattttccc tgctccggca
     4621 gagggtcggg ttcgtaactt gaacgtatca atgtacaggt cagcgtcgag ccgtcaaggc
     4681 aatagccgta tttgctgtcg ttaaaaacag cacagccgcc gattttaccg ttcaaacatt
     4741 ttccggtgac gtcaacaaat ttctgcgacg gcacttcctg ggccggctgt ggtttgcgct
     4801 taattgaccc gaatggaatt tcatacgtac cgacggcgtt attgacagca aacggacatc
     4861 tgagtcgcaa tgcaggaacg ccgatttgag gcccgccgca ttccatccag tctgcctgta
     4921 tggctatcct gacctgcgat tcgccggcgg ataacgaata tctgactttt attgtcgaat
     4981 tgccgacttt atagacgcct tcatacgctg ccgtatgcgg cccgtactgt atcggagcaa
     5041 aagacgtcaa ttcaagagga cagatacttt tttttgcctc tcgtatcagc cacgccgaca
     5101 tgccggctgg ccgctccata acatattcaa gcagcgcagc cggtttttca ggcgacacca
     5161 cgtttatgcc tgttgacctg tcgacaagct cgactatgcc gccggatgcc ctatcgaaaa
     5221 caactcgaac gaattcgttt tccatgccga aagtaccgac atgatagccg gccaacggcc
     5281 gttccgccgc caaacttctg acgcccgcaa caaaatcggt cttctgtaaa tatgaataac
     5341 ccggtgcgtt cgccggcaga ggcaattcat ccggaacgga ttccagaacc agcgaagaat
     5401 aactcatcgc cggaacgctc actggaacag ctatgtcgac atatctatgg ccccacagct
     5461 ggccggaatt caaacgttgt gccggaattt ttaagccgtc cgccgtgcat gccgtaaatt
     5521 gctcagtatg aacgtcatca tgttcggcat cccagacggt aacggatatg acttcgttcc
     5581 gccgacatgc aacaggattg aataccacga cgcctcgctt gtttcccgaa acatggcagg
     5641 ctgaagatat gccgccgcaa tgcgctccac cgaggcccat gccggctccg aattgaacgt
     5701 cgtcttttga cggctcatca acgccggcaa acgaggtatc gatttctttc gcaacagctc
     5761 gcaaagaatt ggttattatc atgccagccg tggcagccgt attttgaaat tggccgctgc
     5821 tatattccct ggtagctttt acgcctgagc ccggcagaat atcgtgaaaa tggccgagca
     5881 gcgtcgcccg ccacgcttcc cgcaattctt cgtaaggata gtttttcttg ccaagaacag
     5941 tatctgcaag aactgcggaa tattcggcac gctcaagcag gcattcagaa agacggttaa
     6001 ccttcttgat atcgctctgc gaggtataac agccggtaaa ttcataattc aattcgtcat
     6061 ttacgaccgg aaaatccgcg gcattttttt cgagaatttc gtaatattca cgagtggtcg
     6121 caaaacggaa attcggataa atcggccagc tgttcatttc ctggcacagg tgcaggtcac
     6181 gccgcgtcgg cccgccgcca gccggacatc gggcaccacg ccggaggcct cgaagcgcgg
     6241 ggccagcatt tcgacgcatt cccgcgcgca ggcgccgagg tccaggcgtt cgggagcggt
     6301 ttcgccgggc cgcgccaggg cgaggaattc ctggaagacc ttttccatgc ggtcgagcgt
     6361 atgccgcatc cggcccgcca gcgccgccat gcggtccgcg cgggcctccg cgccgcgacc
     6421 ggcttccttc tccagcatct gcgcatccag ccgcaggctc gacatcggat tgcggaaatc
     6481 gtgca
//
LOCUS       DAONFI010000101         6460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14045_length_6460_cov_3.487900, whole genome shotgun sequence.
ACCESSION   DAONFI010000101 DAONFI010000000
VERSION     DAONFI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14045_length_6460_cov_3.487900"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLK08_04470"
     CDS             <1..698
                     /locus_tag="PLK08_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPS52789.1"
                     /translation="SFKGKSFSTNETFLEIVTSRGVYSLALSVPILALGWFFSMIISC
                     FVAYYRGTWIDHAGVFICVLGMCMPYLAYMIFGQMFMFWLCPTAAWGIDPISNIYLPV
                     AIAVLAGLGSSVRFYRTVILNEINQDYVRTARAKGVPLPGILFKHVLKNCMLPILTNL
                     VMVVPFLILGNLLLENFFGIHGLGDLMINSISTRDIPIISALTFWTALLYISALFVTD
                     VLYAVFDPRIRLR"
     gene            695..1624
                     /locus_tag="PLK08_04475"
     CDS             695..1624
                     /locus_tag="PLK08_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPS52790.1"
                     /translation="MTLTGYDNSTQHSLRPGKSLWADAASRIFRDRIALICLAVILIY
                     AVIAIIIPFCVDWQKTVNYDHTNEPPSKTFLLGTDAFGRSILIKTMLSAHLSMTVAFW
                     ANIIAVPLGIFIGAVAGYYGGMADHFITWIFTTLSCIPGIILLIALKFALAGKTIPLG
                     FTNLEMDGIAGLVIALSVTNWIDICRLVRAEVMKLREQDFIMAARCTGRSSFAILTIH
                     IIPNVLHLGIITFSLGFVSAITTEVILSYLNLGVQNAPSWGQMINDARMDLIYGRWWE
                     ITSAVIAVFIIVLAWSIFGDRLRDALDPKLKNT"
     gene            1621..2625
                     /locus_tag="PLK08_04480"
     CDS             1621..2625
                     /locus_tag="PLK08_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS52791.1"
                     /translation="MTDNSDILLSVSDLRTYFRTDDGVVPAVDGVSFDIRRGETFALV
                     GESGCGKSVTSYSILKLLQYPPAYHPTGRITFNGEILLDAATCGGKTEKQMRRFRGNK
                     IGMIFQEPMTSLNPVFTVGQQIVEAIRLHKDIRNSQARRLAAELLDQVGLPQPAKSLD
                     AYPHQLSGGMRQRVMIAMALACDPELLIADEPTTALDVTIQAQILELLAEIQSRRDLA
                     ILLITHDLAVVAETAHTVAVMYASKIVEKADVYNLFDEPLHPYTQGLLRAVPKLGQTT
                     DRLEQIPGNVPDPIDFPAGCYFNPRCPKAAECCRQGQNPQLIEVRPGHLVACHCVTGK
                     "
     gene            2628..3407
                     /locus_tag="PLK08_04485"
                     /pseudo
     CDS             2628..3407
                     /locus_tag="PLK08_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            3512..4615
                     /locus_tag="PLK08_04490"
     CDS             3512..4615
                     /locus_tag="PLK08_04490"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPS52792.1"
                     /translation="MDTFIDMDFMLGHWIAENWAAIAIVVTTVSLLVFLVWLLSYYVR
                     ICLNIFCDTPLPLSMNPADFERIEGELVNFRSYDGISLRGMHLCHPEESPYKGTIVFC
                     HEFGSDMYSCGRYVRPLYEAGYDIFTFDFRAHGQSYHDDNYKPLQWPSDKELCDVLGA
                     CAYVENCLTSEGKSAGIGLFGISRGAGSALLAAATDSNIKAIVCDSAFSSETTLLAYM
                     KRWVNIFATVKVVYENHPEAFWRFMQVLVLKCAQCKMKRRFPSVCKALRDMQPRPVFF
                     IHGKRDSYIRVDQSKILHGQAPEPKFLWAVDKAKHNQAVVIQPQKYASRTINFFDKYL
                     CGRQDISDDDIVPGRIEWEGKNGTIPERFEEEC"
     gene            4609..6255
                     /locus_tag="PLK08_04495"
     CDS             4609..6255
                     /locus_tag="PLK08_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPS52793.1"
                     /translation="MLNVGKTLLRPIARVAGFHAWTQLQKYKKSLRKTAEIQSQLLKE
                     LLENFSHSDFSRENGLAFVKNYHDFKNALPIQDYESLKPYINRTLNGDYNAMFSPGEK
                     LLMFSMTSGSTGEPKYIPVTQRFLEGIRFGWNAFGMNMYIQNSNAWLRHIVQLSSAMR
                     EETSAGGLPCGAISGLLAATQKKIVRHMYPAPEWVSSIENVDCRYYAVLRHSVAVDVA
                     FITTANPSSTIKLIETGTKFSEVLLRDIHDGTITLPCDVNLRGHKLKKFKPRPKLAAR
                     IESSIARDGEFMPGHVWNLSLIANWTGGTLRLYMQRLRELFGNIPIYDIGLLASEGRF
                     SIPLAPNTPAGPAEICNNFLEFIPATQRSDEHPDTLLAHELEIGREYFLVFTNWTGFM
                     RYNLDDRVIVRDFIGTCPVIEFLSRGLRTSSITGEKITEHQVVEAMRLASAELDMDVE
                     LFELQGRFLRNNLPRYELRMELDDMAAGTQLAELMDKKLAALNIEYNSKRKSGRLDSI
                     VPLILPKGTMEKAEFNNIKNRHGRAEQYKHQYLITDIMED"
BASE COUNT         1620 a         1786 c         1670 g         1384 t
ORIGIN      
        1 tttcgttcaa gggaaaaagc ttctccacca acgaaacgtt tctggaaata gtaacaagcc
       61 gcggcgtcta ttcgcttgcc ctgtccgtgc cgatactcgc actgggctgg tttttctcca
      121 tgattataag ctgtttcgtg gcgtactacc gcggaacgtg gatagaccac gcgggcgtgt
      181 tcatctgcgt gctgggcatg tgcatgccgt accttgcgta catgattttc ggccagatgt
      241 tcatgttctg gctgtgcccc actgcggcgt ggggaataga cccgatatcg aacatctatc
      301 tgccggtcgc cattgccgtg ctagccggcc tgggcagcag cgtacgtttt tatcgcactg
      361 tcatactcaa tgaaataaac caggactacg ttcggacggc tcgcgccaaa ggcgtgccgc
      421 tgccgggcat actcttcaaa cacgtgctga aaaactgcat gctgccgata cttacgaatc
      481 tggtgatggt ggtgcctttt ctgatactcg gcaatttgct gctggaaaac ttttttggca
      541 ttcacggcct cggcgacctg atgattaaca gcataagcac gcgcgacata ccgataattt
      601 ccgcattgac gttctggacc gcgttacttt acatatcggc gctgttcgtc accgacgtcc
      661 tgtacgccgt ttttgatccc aggatacgac tcagatgaca ctcaccggat atgacaactc
      721 gacacagcat agcctccgcc ccggcaaaag cctctgggcc gacgccgcgt cgcgcatatt
      781 ccgtgaccgc atcgccttga tatgcctggc cgtcatactc atatacgccg tcatcgcaat
      841 cataatccca ttctgcgtcg actggcagaa aaccgtcaat tacgaccaca ccaatgaacc
      901 gccgtccaaa acgtttttac tgggaacaga cgcattcggc cgaagcatac tcataaagac
      961 catgttatcg gcgcatctgt caatgacggt ggcattctgg gccaacatca tcgccgtgcc
     1021 gctgggcata ttcatcggcg ccgtcgccgg ttactacggc ggcatggctg accacttcat
     1081 cacatggatt tttacgacgc tttcgtgcat accgggcata atactgctga tagccctgaa
     1141 attcgcatta gccggcaaaa ccatcccgct cggttttacg aacctggaaa tggacggcat
     1201 cgccggactt gtcatagcat taagcgtgac gaactggatt gacatctgcc gcctcgtccg
     1261 tgcagaagtc atgaaactcc gcgaacagga ctttataatg gcggcccgct gcacgggacg
     1321 cagcagtttt gcaatattga ccattcatat catccccaac gtactgcacc tgggaataat
     1381 aacgttttcg ctgggcttcg tgagcgcgat tacgacggaa gtaatcctca gctacctgaa
     1441 cctgggcgtt cagaacgcac cgagctgggg ccagatgata aacgatgccc gcatggattt
     1501 aatttatggc cgctggtggg agataacatc cgccgtcatc gcggtgttca tcatcgtact
     1561 ggcgtggagc atattcggcg accggctgcg agacgcactt gacccgaaac tcaaaaacac
     1621 atgaccgata acagtgacat tctgctcagc gtaagcgacc tgcggacgta tttccgcacg
     1681 gacgacggcg tagtgccggc cgtcgacggc gtaagtttcg acatccgtcg cggcgaaacg
     1741 ttcgcactgg taggcgaaag cggctgcggc aagagcgtca cgagttattc gatactgaaa
     1801 ctgctgcaat atccgccggc gtatcatccg accggccgga taacgttcaa cggcgaaata
     1861 ctgctcgacg cggcaacatg cggcggcaag accgaaaaac agatgcgacg ttttcgcggc
     1921 aacaaaatcg gcatgatttt ccaagagccc atgacatcgt tgaatccggt tttcaccgtc
     1981 ggccaacaga tagtggaggc gatacgcctg cacaaggata ttcgcaattc gcaagccagg
     2041 cggctggcgg ctgaattgct tgaccaggtc ggcctgccgc agccggcaaa atcgctcgat
     2101 gcatacccgc atcagctttc cggcggcatg cggcagaggg taatgatagc aatggcgctg
     2161 gcatgcgacc ctgaactcct catcgccgat gaacctacga cggctctgga cgtcacgata
     2221 caggcacaga tactcgaact gctggctgaa atacagtcgc ggcgggattt ggcgattctg
     2281 ctcatcacgc acgacctggc ggtcgtggcg gagacggcac atacggtagc cgtcatgtat
     2341 gcgtcaaaaa tcgtggaaaa ggctgatgtt tataatcttt tcgacgaacc attacacccc
     2401 tacacgcaag gccttctgcg ggctgtgccg aaactcggcc aaaccacaga cagactcgaa
     2461 caaataccag gcaacgtacc cgacccgatt gacttcccgg cagggtgtta tttcaatccg
     2521 cgatgcccga aagctgccga atgctgcagg cagggacaga atccgcaact aattgaagta
     2581 cggcccggcc acctcgtcgc ctgccattgc gtaaccggaa aatgattatg gcccaaaaat
     2641 ccgacatatt gctgcaggtt gacagcctga cgaaaatctt tgcaaggcgc ggcatggaag
     2701 tgcatgccgt cgataatgtg agttttaacg tcgcacgcgg cgaaacgctg gggcttgtgg
     2761 gcgaaagcgg cagcggcaag acgactgtcg gccgttgcat aacgcggctg attgaaccga
     2821 cggctggaaa agtgactttc ctagatgacg acatctcaac gctgcgcggc caacggctaa
     2881 ggagcacgcg taaagacctt cagatgattt tccaggaccc tgcgtcatcg ctcaatccca
     2941 ggatgacagt cggcggaata atcgccgaac cgcttaaggt gcatttcagg atgaatggcc
     3001 gacaacgcag gatggaggtc agccgcctgt tggaacgggt cggcatgtcg ccagcctacg
     3061 ccagccggta tccccacgaa ttctcaggcg gccaacgaca gcggataggc atagcccgcg
     3121 ccatagcaac tcagccgaaa ctcataatct gcgacgaacc agtcagcgcc ctggacgtat
     3181 ccatacaggc acagatactc aacctgctgg ctgacctgca aaaggaactg aaactgagtt
     3241 atctgttcat cgcccacaac ctggcggtag tggaacactt cgccgacaga atcgccgtga
     3301 tgaaaaacgg gaaaatagtc gaaagtgcag acaaagccga gatttacgca tcgccccaga
     3361 atgaatacac ccgctcgctg ctggcgtctg tgccgtcgat agacccgcac aacccgcatt
     3421 tggcggcgag aaagaaaccc actgtctgaa accttgtttt tgcccggccg gttttgtatc
     3481 atttacgggt tcataaggag acatttctgc gttggatacg tttatagaca tggactttat
     3541 gcttggacat tggattgcgg aaaactgggc cgccatcgct atcgtcgtta cgacggtcag
     3601 cctgctggta tttctggtct ggctgctgtc atattacgtg cgaatctgcc tgaacatatt
     3661 ctgcgatacg cccctgccgc tgtcgatgaa tcctgccgat ttcgagcgta ttgaaggcga
     3721 acttgtgaat tttcgcagct atgacggcat cagccttcgg ggcatgcatc tctgccatcc
     3781 ggaagaatcg ccgtacaagg gcactatcgt attctgtcat gaattcgggt cggacatgta
     3841 cagctgcggc cgatatgtcc ggccgcttta tgaagcgggc tacgacatct tcacattcga
     3901 cttccgcgcc cacggccaaa gctatcacga cgacaactac aaacctcttc agtggcccag
     3961 cgacaaggaa ctgtgcgacg tcctcggcgc atgcgcctac gtggaaaact gtctgacttc
     4021 ggagggcaaa tccgccggca taggcctgtt cggaataagc cgcggcgccg gctcggcgct
     4081 gcttgccgcc gcaactgaca gcaacataaa agccatcgtc tgcgacagcg ccttcagttc
     4141 cgaaaccaca ctgctggcat atatgaaacg ctgggtaaac atcttcgcca cagttaaggt
     4201 tgtttacgaa aatcatcccg aggcattctg gcgattcatg caggtactcg tgctcaagtg
     4261 tgcgcagtgc aagatgaaac gacgtttccc ctcggtctgc aaggctcttc gcgacatgca
     4321 gccgcggccg gtgtttttca tacacggcaa acgcgacagc tatatccgcg tggaccaatc
     4381 aaagatactc cacggccagg cgcctgagcc caaattcctc tgggccgtgg ataaggctaa
     4441 acacaatcag gcggttgtaa ttcagccgca gaaatacgca agtcgaacaa taaacttttt
     4501 tgacaaatat ctttgtggcc gtcaggacat ttctgacgac gatatcgtgc caggcagaat
     4561 cgagtgggaa ggcaaaaacg gcacgatacc ggaaaggttt gaagaagaat gctaaacgta
     4621 ggcaaaacat tgctgaggcc tattgcgagg gtggcgggat ttcacgcatg gacacagctt
     4681 cagaaatata aaaaatcact gagaaaaacc gccgaaattc aaagtcaact tctgaaggaa
     4741 ctgctggaga atttttcgca tagcgatttt tcacgcgaaa acggcctggc gttcgtgaaa
     4801 aactatcacg acttcaaaaa tgccctgccg attcaggact acgaatcact caagccgtac
     4861 atcaatcgta cgctgaacgg cgactacaac gccatgttca gccctggcga aaaactgctg
     4921 atgttctcca tgaccagcgg ctcaaccggc gaaccgaaat atattcccgt cacgcaacga
     4981 tttctagagg gcatacgttt cggctggaat gctttcggca tgaatatgta catacagaac
     5041 agcaatgcat ggctgagaca tatcgttcag ctttcgtcag ccatgcgtga ggagacctca
     5101 gccggcggct tgccgtgcgg cgcaataagc ggcctgctgg ctgccacaca gaaaaaaatc
     5161 gttcgccata tgtatcctgc gccggaatgg gtctcgtcga tagaaaatgt cgactgccgt
     5221 tattacgcag tcctgcgtca ttcggttgcc gtcgacgtcg cgttcattac gacggctaat
     5281 ccgtcgagca ccataaagct catagagacc ggcacaaaat tctccgaagt tctgttgcgg
     5341 gacatacatg acggcaccat aacgctgccg tgtgacgtca acctgcgcgg ccacaagctg
     5401 aagaaattca agccacggcc aaaacttgcc gcaaggatag aatcatccat cgcccgcgac
     5461 ggcgagttta tgcccggaca cgtctggaat ctttcgctaa tcgccaactg gaccggcggc
     5521 acattgcggt tgtatatgca gcgtctccgc gagcttttcg gcaacatccc catatatgac
     5581 ataggcctgt tagccagcga aggccgcttc agcattccgc tagccccaaa cacccctgcc
     5641 ggcccggcgg agatatgcaa caacttttta gagttcattc cggcaacgca gcgcagcgat
     5701 gagcatccgg atacgcttct cgcccacgag cttgaaattg gcagggaata tttcctggtc
     5761 ttcacgaact ggacaggctt tatgcgctac aacctggacg accgcgtaat cgtgcgggat
     5821 ttcatcggca catgtccggt aatagaattt ctttcgcgag ggcttcgcac gtccagcata
     5881 accggcgaaa aaatcactga acatcaggtc gtcgaggcaa tgcggctagc cagcgcggaa
     5941 ctggacatgg acgtggaact gttcgaactg cagggccgtt tccttcgcaa caatctgcca
     6001 cgttacgaat tgcggatgga acttgacgac atggcagccg gcacacagtt ggcggaactg
     6061 atggacaaaa aactggcggc tctgaacatt gaatacaatt ccaaacgcaa gagcggacgg
     6121 ctagacagca tagttccgct gatactgccc aaaggcacaa tggaaaaagc cgaattcaat
     6181 aacatcaaaa atcgtcacgg acgtgcagaa caatataagc atcaatacct gataaccgac
     6241 attatggaag attagagcat attaaacctg actttgcgtc tgctgccgtt tgcggcggcg
     6301 ttttgctgcg ttctccacga tgcagctcaa gcaatttcaa atttaacatg ctctagagaa
     6361 aggcccgaca atgcagaaaa aagccactga attcctctca cccgacgacg gacgtatacg
     6421 actgatgttt cagggggcgg caaaaacggt gacaggctcg
//
LOCUS       DAONFI010000102         6439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14106_length_6439_cov_3.864348, whole genome shotgun sequence.
ACCESSION   DAONFI010000102 DAONFI010000000
VERSION     DAONFI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14106_length_6439_cov_3.864348"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLK08_04500"
     CDS             complement(<1..279)
                     /locus_tag="PLK08_04500"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HPS52794.1"
                     /translation="MSYATKSEIESVFGKSNVASWSNLDNEMSGADDTRIASAISYAD
                     SIINARLRGGRYALPLVNSQGTVPGLVKDWAAKLAGVWLYECRGLFDDD"
     gene            complement(360..1457)
                     /locus_tag="PLK08_04505"
     CDS             complement(360..1457)
                     /locus_tag="PLK08_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HPS52795.1"
                     /translation="MSATLQQVLGGENLCGVIQGIKSGTGDDLPQDFFRVDGKCEGNT
                     GKYFRVDGDRKTARIAQYGSPSKRRNLKGVTEIPVTLLHTIEHIIHNVTTLQNLESVN
                     GGKQQLGREQVARDTADFKRRFIELRKACVRMAIASGQVHFDSDGNLLSSSTGAAFSI
                     DFGVPAGNKNQLNVFGDGAIISGSWASANTGIITHIKKIKKASKKLTGYEIGCAFYGA
                     SIIDYFLANTQVTKLIANNSATQLVLQGGEIPDGFLGIKKWYPMDSSFYEDDDGTIQE
                     IWGDDNITFTPTPSLEWFGFLEGSYPVPKTLDIVKDAAAALDNTMQVYGQFSYATLQD
                     DPVSAKQVAGDTFLPVLKVPKAVFQAKVANF"
     gene            complement(1534..2538)
                     /locus_tag="PLK08_04510"
     CDS             complement(1534..2538)
                     /locus_tag="PLK08_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52796.1"
                     /translation="MTAYSSMPGLGTEKTATPRCLLAAGVGTYLAGGRVINGSKSRDL
                     TNTGDTDILQAGLLMGKITATKKYSPSIIGVLASAYNGGTSMTVSAAVAAEIVRRIGA
                     SGTLVVTGPATAGGEVRSQSIVFSAVNTTTGVITVTAADATTVQTVAISGTLSAGTFK
                     LIVPSAAGAMVETADIAYNANIAAIQSAVDTALGSGKVTVGGTDVSSFTLTYGSDYDG
                     VDVPLADVDTDGLTGETGASVTPTTIGSVGDIIAGGFVGANDGSAAPKCFVAEDFGIK
                     VTDDDDEDIDVQFSKMLVGGNVNTSMIINYPTDAALVAWVKAQLRSVGHAYTFTDDFE
                     "
     gene            complement(2597..3763)
                     /locus_tag="PLK08_04515"
     CDS             complement(2597..3763)
                     /locus_tag="PLK08_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52797.1"
                     /translation="MESTKRLLRADNGSRFISASAQSLNKTGQPAQKFLKDMIRCGVY
                     KHPVHGWTLDVTPQRMDNWVAAFNRMKENGVDVEVPIDHSMSAEDNRGYVVDMFRGGT
                     EAAKDYPQATDPKTLYGVHELIGDDSIELASRCKNVSVLIERDFVDGQGVAYGEAITH
                     SSIVQQPIVPGQGDFIPLSKAGDVNAPIPVFVLSSEASKGKNKKNNVENTNMELFDGL
                     KQQFGFGDDVTEENILSRIKESVDKTAEDNKTLSKQVTELNAKVESLTAKVDDGKAAS
                     KIDPDALDGLVEGTEARIEKLQACGKLTPAAAKVAHDVLIGEPGKRNTLMLSRKASGM
                     DESIAKKVLSIVEANDPKELGEMTKSQTLSRQTPGEQPPAVDAEIQKSMIKACE"
     gene            complement(3826..4215)
                     /locus_tag="PLK08_04520"
     CDS             complement(3826..4215)
                     /locus_tag="PLK08_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52798.1"
                     /translation="MDKDKKPWDDIERCKRFVGAPDEVRIGDATKKTHSPKPSLKKTK
                     KGEEGMMRATAVDKSGQVHVFSLACDDPADAFADLMQWWAKLTKGKPQETLESVKFEP
                     DTVNLPFLGEASLSNLFLSAAATKDFE"
     gene            complement(4219..5700)
                     /locus_tag="PLK08_04525"
     CDS             complement(4219..5700)
                     /locus_tag="PLK08_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HPS52799.1"
                     /translation="MCAASKYARRQAQIYDNDCRQIEDASQVAVKKITRKIRLVGINS
                     WNQSGNIDSVVNAVRQAVHEEAGGFLADLMVAAHLRSSLRAIQTATDFLQKRKELYTV
                     KDNPYKKARDYLVRRMDLKDREVESLNLYYTREAVNVTNAADELLERNLRQAAVEAVG
                     KGAHTREGVAIFRDAFEKSGINPESPWLLETLVRTQTQLAYSAGRWNVNDDSAVQEIL
                     WGYEYVTVGDDRVRPSHAALDGCRYAKDDPIWNKIFPPNGYNCRCTTIEIFKGDRLAN
                     EKPAKPIDIDGQKVFPSADKGWNFNPGRVGQDMAKIELPPNPQPSVRRIIPQMTTGKP
                     MTMADQKNVIDYTSTDYMSIVRAQLGKPMRGQNMTKEQALEKAHAVEAALFKLPGKEA
                     VSYRGMAFKSARAKADFLASLQTEGKYTFEAFQSASLLKEFPEKLAKDRKRGLLFMID
                     GKTGRDISKYSLHPEEKEILFLQGVECKIEAIIDNYIVHCREV"
     gene            complement(5684..>6439)
                     /locus_tag="PLK08_04530"
     CDS             complement(5684..>6439)
                     /locus_tag="PLK08_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52800.1"
                     /translation="KVAGVIPMIEYPPGESLDQNGNLKTNYELAAMILANLGKGNGVA
                     MPNVLAKYAEDLARQGVDISQLKAWMISFLEVQGSHGDMFEKLMRHRESLMMRGWLVP
                     ERTATEGQHGTNAEAVTHGELSLVVADQTLLEMLQAVNWHLVNPLLRTNFGQQAENKV
                     WISRSGLDPEQKAFLRNIMQSILTNPANVDLFTTMLDVDAMLDDTGLPKGSDIGNLAK
                     DIADRNRDLPQTNSMAASMRALYEKVHRDVCRE"
BASE COUNT         1381 a         1754 c         1512 g         1792 t
ORIGIN      
        1 gtcgtcatcg aaaagcccgc ggcattcgta cagccacacg cccgccagct tggcggccca
       61 gtcttttaca aggcccggaa ctgtcccctg cgaattgacg agcggcaagg cgtatctacc
      121 gcctctcaat cgggcattta taatcgagtc ggcatacgaa atcgcggacg ctatgcgggt
      181 atcgtccgct cccgacatct cattatccag gttcgaccag ctcgcaacgt tcgatttgcc
      241 aaatacgctt tcgatttcag atttagtggc gtagctcatt ttttaaacct caaaaaaagg
      301 caggtggaat cgttttttaa aacgccacct gccttgataa ttatttgtcc attgtttgac
      361 taaaagttcg caaccttagc ctggaagacg gctttcggca ctttcaggac cggcaggaac
      421 gtatcacctg caacctgctt cgccgacacc ggatcgtcct gcaacgtcgc gtagctgaac
      481 tgcccataga cctgcatggt attgtccagt gccgcagcgg cgtccttgac gatatccagc
      541 gtcttcggta ctgggtacga gccttccagg aagccgaacc attcgaggct gggcgtcggt
      601 gtgaaggtga tgttgtcgtc gccccagatt tcctggatag tgccatcgtc gtcttcgtaa
      661 aaagacgaat ccatcggata ccatttcttg attcccaaga atccatccgg aatttcgccg
      721 ccctgcaaca ccagttgtgt cgcggagttg ttggcgatga gcttcgtcac ctgcgtattg
      781 gcaaggaaat aatcaatgat acttgcgccg taaaacgcac agccgatttc gtaaccagtg
      841 agtttctttg acgccttctt gattttcttg atgtgcgtta tgattccggt attggcactt
      901 gcccaactgc cgctgattat agcaccgtcg ccgaaaacgt tcagctggtt tttattgccg
      961 gccggaacgc cgaaatcgat ggagaacgcc gctccggtgc tactggacag aaggttgccg
     1021 tcgctgtcga aatgcacctg gccgctggca atcgccatgc ggacgcatgc tttgcgaagt
     1081 tcgatgaatc ggcgcttgaa gtcggccgtg tcgcgggcaa cctgttccct gccaagctgc
     1141 tgcttgccgc cattgaccga ttccagattc tgcagcgtcg tcacattgtg gataatgtgc
     1201 tcgatggtgt gcagcaacgt taccggtatt tcggttacgc ccttgagatt ccggcgtttc
     1261 gacgggctgc cgtactgggc gatacgcgca gtcttgcggt caccgtccac gcggaaatat
     1321 ttaccagtgt tgccttcgca cttgccgtca actctgaaaa aatcctgtgg gaggtcatcg
     1381 ccagttccgg acttgattcc ctgaataacg ccgcagaggt tctctccgcc caatacctgc
     1441 tgaagtgtag cactcatttt ttcttactcc aaaattttat ttgtaatctg ttgttgtaaa
     1501 aaattttaaa aggttataaa aaccttttaa acgttactca aaatcgtccg tgaacgtgta
     1561 tgcgtgaccc acgctgcgga gctgggcttt cacccacgca accagagccg catccgtcgg
     1621 atagttgata atcattgacg tattgacgtt gccgccgaca agcatcttgg aaaactggac
     1681 atcaatgtcc tcatcgtcgt cgtccgtcac ctttatgccg aaatcttcgg cgacaaagca
     1741 cttcggggcc gccgacccat cattcgcacc cacgaaccca ccggcgataa tatccccgac
     1801 gctgccaatc gtggtgggag ttacgctcgc tccggtttcg cctgtcaggc cgtcggtatc
     1861 gacgtcagca agcgggacgt ccacgccgtc ataatcgctg ccgtaagtca acgtaaagct
     1921 actcacatca gttccgccaa cagtaacttt gccagaacca agggccgtat cgactgcact
     1981 ctggatagct gcgatattcg cgttgtaggc aatatctgcg gtttccacca tcgcccctgc
     2041 cgcggaaggc acaatcagtt tgaacgtgcc agcagacaac gtaccggaaa tggcgacggt
     2101 ctgtaccgta gtcgcatccg ccgccgtgac ggttatcacg ccggtagttg tgttgactgc
     2161 ggaaaagact atgctctggc tgcggacttc accgccggcc gtcgctgggc cggtaactac
     2221 cagcgtgccg cttgcaccga ttcgacggac tatttcagcc gcaacagccg cggaaacagt
     2281 cattgaagtc ccgccgttgt acgcggacgc gagaacgccg ataatcgacg gggaatattt
     2341 tttcgtcgcg gtgattttgc ccatgagcaa gccagcctgc agaatatcgg tatcgccggt
     2401 attggtcagg tcacgcgatt tgctgccgtt tattacacga ccaccggcaa gatacgtacc
     2461 aacgcctgct gcgagcaggc aacgtggagt cgcggttttt tcagttccca atccgggcat
     2521 tgagctataa gctgtcattt ttgtatctcc aattttttct ggttaatttt ttttgaaaaa
     2581 tggtctgctg aaaaaattat tcacaggcct ttatcatgga tttctggatt tccgcatcga
     2641 cggctggcgg ctgttctcca ggggtctggc gactcaaagt ctgtgacttc gtcatttcgc
     2701 caagttcctt cggatcgtta gcctcaacaa tcgacagcac tttctttgcg atggattcgt
     2761 ccatgccgct ggccttgcgg gacagcatga gagtgttgcg tttgccaggc tcgccgataa
     2821 gcacgtcgtg tgcaacttta gcggccgccg gagtgagttt cccgcatgcc tgtaatttct
     2881 cgatacgggc ttccgtgcct tccactaggc cgtccaaagc atcagggtca atcttgctcg
     2941 cggccttacc atcatcaacc tttgcagtca agctctcgac ttttgcattg agttccgtga
     3001 cctgcttcga gagggttttg ttgtcctctg cggttttgtc cacactttcc ttgatgcggg
     3061 aaaggatatt ttcttcggtg acatcgtcgc cgaatccgaa ttgctgtttg agtccatcaa
     3121 agagttccat gttcgtattc tccacattgt tttttttatt tttgccttta ctggcttcag
     3181 atgataatac gaatacaggt atgggagcat tcacgtcacc agctttggat aagggtataa
     3241 agtcaccctg gccgggtact atgggctgtt gtacaatgct ggaatgtgtg attgcttccc
     3301 cgtaagcaac cccttgcccg tcaacaaaat cgcgttcaat caacacggac acgtttttac
     3361 accggctcgc aagttcgatt gagtcatcgc cgataagttc gtggacgccg tacaacgtct
     3421 ttggatctgt tgcctgggga taatctttgg ctgcttccgt gcccccgcgg aacatgtcga
     3481 cgacgtatcc ccgattgtct tccgcggaca tagagtggtc tatcggaact tcaacgtcga
     3541 ctccgttttc tttcatgcgg ttgaatgccg caacccaatt atccatcctc tgcggcgtca
     3601 catcgagcgt ccagccgtgc actgggtgtt tgtagacccc gcaacgtatc atatctttca
     3661 agaatttctg ggcgggctga cctgttttat tcagggattg agctgacgcg gaaatgaacc
     3721 ggctgccgtt atcggcccgt aaaagtcttt tggtagattc cattttgtat cctttgcaaa
     3781 tttctcatag aagaatttac agaggatacg caggaaaaag gtgtgttatt caaaatcttt
     3841 agtcgcggct gcacttaaaa acagattgga cagcgacgct tcccctaaaa acggcaggtt
     3901 tacagtatcc ggttcaaact taactgactc aagcgtttcc tgcggtttgc ccttggtaag
     3961 tttcgcccac cactgcatga ggtctgcaaa tgcatcggca gggtcgtcgc atgccaggct
     4021 gaaaacgtga acctgccccg atttgtcgac tgccgtcgcc ctcatcatcc cttcttcgcc
     4081 ttttttagtt tttttgaggc ttggtttggg ggaatgggtt ttcttcgtgg cgtcgcctat
     4141 ccgaacctcg tcgggagcgc ctacgaatct tttgcaacgt tctatgtcat cccacggttt
     4201 cttgtcttta tccatttatc agacctctct gcaatggacg atataattat caattatcgc
     4261 ctcgatttta cactctaccc cctgtaaaaa caatatctct ttctcttccg gatgaagcga
     4321 atacttggat atatcacgac cggttttccc atcaatcata aacagcaatc ccctcttgcg
     4381 gtctttggca agtttttcgg gaaattcttt caatagcgat gcactctgga atgcctcgaa
     4441 cgtgtatttt ccctccgttt gcaacgacgc taaaaaatca gccttagccc gcgccgattt
     4501 aaacgccatt ccgcgatagg atacggcttc tttgcctggc aacttaaaca gagcggcttc
     4561 aaccgcatgc gccttttcaa gagcctgctc tttagtcata ttctggcctc tcatgggttt
     4621 accgagctgt gctctgacga ttgacatata atctgtagac gtataatcga ttacattttt
     4681 ctggtcagcc atcgtcatcg gtttgccggt tgtcatttgc gggattattc gcctaactga
     4741 cggctgtgga tttggcggca gttctatttt cgccatatct tgaccgactc gcccaggatt
     4801 gaaattccat cctttgtcag ctgaaggaaa aactttttgt ccgtcaatat ctattggttt
     4861 ggctggtttt tcgtttgcaa gcctatcacc cttgaatatc tcaatggtcg tacagcgaca
     4921 gttataacca ttcggcggaa aaattttgtt ccagattgga tcgtcttttg cataccggca
     4981 accatccaat gccgcatggc tcggcctcac gcggtcatcg ccgacagtaa cgtattcata
     5041 gccccacaag atttcctgta cggccgaatc gtcgtttacg ttccacctgc cggcggaata
     5101 cgcaagttgc gtctgtgttc taacaagcgt ttcaagcagc cacggagatt ctggatttat
     5161 tcctgatttt tcaaatgcat ccctgaatat agccacgcct tcgcgtgtat gcgcaccctt
     5221 accgacggct tcgacggcgg cttgcctcaa attcctttca agcaattcgt ccgccgcatt
     5281 ggtaacattg accgcctccc gcgtataata aagattgagc gattcaactt cgcggtcttt
     5341 caaatccatc cgcctgacaa gataatctct ggcctttttg tatgggttat ctttgactgt
     5401 gtagagttct tttcgcttct gcagaaaatc cgtcgcagtc tggattgctc gtaggctact
     5461 gcgcagatgg gccgctacca tcaggtcggc taaaaatcct cctgcctcct cgtgcacagc
     5521 ctgacggaca gcgtttacaa cgctgtcaat atttccagat tgattccaac tgtttatgcc
     5581 gacaagtctg atttttctgg taattttttt aacagcaacc tgcgaagcat cttcaatttg
     5641 ccggcagtcg ttatcgtata tctgggcctg cctgcgggcg tatttactcg cggcacacat
     5701 ctctgtgcac cttttcgtac agggctctca tacttgcagc catcgagttt gtttgaggta
     5761 aatccctgtt gcggtctgcg atgtctttcg ccagatttcc aatatcgctg cccttgggca
     5821 ggcccgtatc gtccagcatg gcgtcaacgt ccagcatcgt cgtaaataaa tccacgttcg
     5881 ctggattagt caaaatcgat tgcatgatgt tccgcagaaa cgccttctgt tcagggtcta
     5941 aaccgctgcg ggatatccaa accttatttt cagcctgctg cccgaaattc gtccgcaaca
     6001 acggatttac caaatgccag ttgacggctt gcagcatttc caaaagcgtc tggtctgcaa
     6061 caaccaggct caattctcca tgggtcactg cctccgcgtt tgttccgtgc tggccctccg
     6121 tcgcagttct ttccggcacg agccaacctc gcatcatcag gctttcgcgg tgacgcatga
     6181 gcttttcaaa catgtcaccg tgactgccct gaacttccaa aaatgatatc atccacgcct
     6241 tgagctggga aatatccacg ccctgccgcg ccaaatcttc agcatatttc gccagtacat
     6301 taggcatggc aacaccgttt cccttgccca gattggcaag tatcatggct gccaactcgt
     6361 agtttgtttt cagattccca ttttggtcaa ggctttcacc aggcggatat tcaatcattg
     6421 gtattacgcc ggcgacttt
//
LOCUS       DAONFI010000103         6421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14152_length_6421_cov_4.313541, whole genome shotgun sequence.
ACCESSION   DAONFI010000103 DAONFI010000000
VERSION     DAONFI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14152_length_6421_cov_4.313541"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /gene="nuoF"
                     /locus_tag="PLK08_04535"
     CDS             complement(<1..1905)
                     /gene="nuoF"
                     /locus_tag="PLK08_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPS52801.1"
                     /translation="MAEIKSSNEVELVKLWADGPISHFAPELREKLQKKIDTMSKSRD
                     EKAVIYIGAGTCGLGAGAAGTIAATKKYLADKKVDAEIVEVGCIGLCAVEPMLDVQLP
                     GRQRMCFQKVTSDKVDSILDAFFGKAEMPADLVFGQHRQPSRQEWAGKPYLDEHPFFA
                     PQTRWVLKNCGIIDPTSIEEYIALGGYSAMARTLTNRTPDEVCDEIEKSGLRGRGGGG
                     FPTGKKWKFARAQTAEQKYLICNADEGDPGAFMDRAVIEGDAHRLLEGMAIAAYAIGA
                     TKAYVYIRAEYPLAIERLIQAIDQAKSYGLLGRNIMDSGFDLEIIIKKGAGAFVCGEE
                     TALIHSVEGKRGMPRPRPPFPAVSGLFGKPTIINNVETLANLPLIMANGYQWFSSLGT
                     AGSKGTKVFALSGKISLTGLVEVAMGTPLRKVIFDIGGGIANGKKYKAVQIGGPSGGC
                     IPEQHLDIEIDYESLKTVGAMMGSGGLVVMDEDTCMVDVAKFFMDFIQRESCGKCIPC
                     REGTRRMLETLKRITSGRASEAQKPHGALLRFQSVMYLERLANTIKDTSLCGLGQTAP
                     NPILSTLRFFRNEYEAHVFERRCPAGACKELVKYSIDASKCVGCTACARKCPANAIMG
                     AAKSPHYIVPD"
     gene            complement(1898..2377)
                     /gene="nuoE"
                     /locus_tag="PLK08_04540"
     CDS             complement(1898..2377)
                     /gene="nuoE"
                     /locus_tag="PLK08_04540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPS52802.1"
                     /translation="MAVKIEDILEKYSGAGRDKLIPLLQDVQDAQGYLSREAVTAIGR
                     QLNLPTSKIYGVATFYNQFRFQPRGRYHVQVCRGTACHVKGSANILEAVKRALGIEAG
                     QTARDGSFSLEVVACIGACGLAPVINVNGNFHAKLTPESVVKVLNEYRAKDVEKANG"
     gene            complement(2439..3056)
                     /locus_tag="PLK08_04545"
     CDS             complement(2439..3056)
                     /locus_tag="PLK08_04545"
                     /inference="COORDINATES: protein motif:HMM:NF003996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPS52803.1"
                     /translation="MVSERVIGRLSLYRRILANLREDGQRSVFSHQLAVLAGCTPAQV
                     RRDMMVLKTEGTPTRGYNIEKLLEALDEILVGSEIRKVLLVGIGHLGRAVLSYFQDRQ
                     SNLVIAAAFDRDEAVTGRVISGCRCYTMDKLKEVIDSEDIRVAMLAVPASGAQAVADM
                     LIEMGIRGFLNFAPVHLKINPPAYVEHVDIMTALERVIYFTQLKK"
     gene            complement(3486..4700)
                     /locus_tag="PLK08_04550"
     CDS             complement(3486..4700)
                     /locus_tag="PLK08_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HPS52804.1"
                     /translation="MRFLKAVILLVVLFDAAVAENFTEPTRKTLPVKFRPFKTTHYVI
                     YTDWDICDSMIARIRLECNYRSFRRLAVKLYRPMPGRCNVYLFADVNDYYAAGGFPNT
                     RGCYIWTRMGKRNPKVWLMAWLNGSVFEVMQHEGFHQFAMNAFPQIPLWLNEGLAEYF
                     NYAKWTGDELLEGFVSANQLAIVRNALLKNAFAPMKKFMTISDEEWKNGLRSEANLNY
                     CQAWSIVHFFMHADDGKYRENFEKFLCQLARGTKADEAWKSCFAENVETIEAEYKNWW
                     LTKAEETAELDSIACARIFISFIGRNFTKGYQPINFETFVKDANDGRLKLAVSQDKEN
                     YLPDSLLNWALDNYSKAGEYFIGCDADGQPFVRLKLPESGTYIYTFKPRSVGVNVIEK
                     HIPRVKTAPAAP"
     gene            complement(4752..5918)
                     /gene="hemW"
                     /locus_tag="PLK08_04555"
     CDS             complement(4752..5918)
                     /gene="hemW"
                     /locus_tag="PLK08_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPS52805.1"
                     /translation="MLKKADSLYIHVPFCVRKCKYCDFYSVSVDDELVGRYVEALERE
                     LQLYTGMLSRPLATIYVGGGTPTSLSMPHLRRLLGNLKKFVGPGTEYTIEANPGTLDN
                     EKIGLCRFMNINRVSLGAQSFHDDELAGIGRIHTRREIFDSFKMLRDAGFDNINLDLI
                     FALPGQSLKKWRESLRQAVRLSPEHISCYALSYENGTEFHTQLSNGELQEIEDEEQLR
                     MYNTAIYTLINAGFEHYEISNFARPGLRCRHNLVYWFNYPYIGIGPSAASSFIHDSFT
                     TRRTNIRDVRRWISAMLDDSIPSPGETETLNMNMTMAETLMLRLRMREGMRLTNFGYR
                     FGMSFEDAFPYSISTHLASGGLAMNGEKLMIPRERLFVANTVLADIVHEAAEKK"
     gene            complement(5902..6360)
                     /gene="ruvX"
                     /locus_tag="PLK08_04560"
     CDS             complement(5902..6360)
                     /gene="ruvX"
                     /locus_tag="PLK08_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPS52806.1"
                     /translation="MPRYLGVDYGHKRVGLAVGDSADGFATPLCVLQGGDDESLVRKI
                     SDVCEEYDCRVVIVGLALNMDDTAGPAAMKTLEFAQKLCVGGLDVRMWDERLSSFDAD
                     MTLAGTMTRKKRKARQDAVAAASFLNDFLAGKNRQAAQTPQQVLNNVKKS"
BASE COUNT         1497 a         1783 c         1517 g         1624 t
ORIGIN      
        1 atctggcact atgtagtgcg ggctcttcgc agcgcccatg atggcgttcg ccggacattt
       61 gcgggcacag gcagtacagc cgacgcattt tgatgcgtcg atactgtatt tcaccagttc
      121 cttacacgcg ccggcaggac aacgtcgttc aaacacatgc gcctcgtatt cgttacggaa
      181 gaaccgcaac gtggaaagaa tcggattcgg agccgtctgg cccaacccgc aaaggctggt
      241 atccttgatg gtattcgcca agcgttcgag atacatgacg ctctggaaac gcaaaagtgc
      301 tccgtgcggt ttctgtgcct cgctggcccg gcccgacgtg atgcgtttca gcgtttcaag
      361 catccggcga gtgccttcgc ggcacggtat gcatttgccg cagctttcac gttgaatgaa
      421 atccatgaaa aacttggcga cgtccaccat gcaggtgtcc tcatccatca cgacaaggcc
      481 gccgctgccc atcatcgcac cgacggtttt cagagattcg taatctatct cgatatccag
      541 gtgctgttca ggtatgcagc cgcctgaggg gccgcctatc tggacggctt tgtacttctt
      601 gccgttggct atgccgccgc cgatgtcgaa aatgaccttg cgcagcggcg tacccatcgc
      661 cacttcaaca aggccggtaa ggctaatctt gcctgacagc gcgaaaacct tcgtgccctt
      721 cgagccggct gtgccgagcg aagaaaacca ctgataaccg ttagccatta tcagcggcag
      781 attagccagc gtttcgacgt tgtttataat cgtcggcttg ccgaaaaggc cgctcaccgc
      841 agggaacggc ggccgcggcc gcggcattcc acgcttgccc tcgacactgt gtatgagggc
      901 cgtctcttcg ccgcagacga acgcgcctgc gcctttcttt atgattatct ccaggtcgaa
      961 accgctatcc ataatgttgc ggccaagcag gccgtaactc ttcgcctggt ctatagcctg
     1021 aatcagacgc tcgatagcca gcggatattc ggcacggatg taaacgtagg ctttcgtggc
     1081 accgatggca tacgccgcaa tcgccatgcc ttcgagcagc cgatgcgcgt cgccttcgat
     1141 gacggcacga tccataaacg cccctgggtc gccttcgtca gcgttgcaga tgaggtactt
     1201 ctgctcagcc gtctgagcgc gggcgaattt ccacttcttg cccgtcggga atccgccgcc
     1261 gccacgccct cgcaagccgg acttttcaat ttcgtcacaa acctcatccg gcgtcctgtt
     1321 cgtaagagtg cgggccatcg cggagtatcc gcccagtgcg atgtattcct ctatgctcgt
     1381 ggggtcaata atgccgcagt tcttcaacac ccatcgcgtc tgcggggcaa agaacggatg
     1441 ttcgtcgaga tacggcttgc cggcccattc ctggcggctt ggctggcgat gctggccgaa
     1501 aacgaggtca gccggcattt cagccttgcc gaaaaacgca tcaagtatgc tgtcaacctt
     1561 gtcggaggtg actttctgga agcacatgcg ctgccggcct ggcaattgca catccagcat
     1621 cggctcgacg gcacagaggc ctatgcagcc gacttccact atctcggcgt caactttttt
     1681 gtcggcaaga tattttttgg tggcggcgat ggtcccagcc gcaccggctc caaggccgca
     1741 tgtgccggca ccgatgtata tgaccgcctt ttcatcgcgg ctttttgaca tcgtgtcgat
     1801 tttcttctgg agtttttccc gcagctccgg cgcaaaatgg ctgataggcc catcagccca
     1861 gagcttcacg agttcgactt cattactgct ttttatctca gccatttgct ttctccacat
     1921 ctttggcgcg atattcgttc aacaccttca ctactgattc cggcgtaagt ttggcgtgaa
     1981 aattgccgtt cacatttatg accggcgcca ggccgcaggc tccgatgcag gctacgactt
     2041 caaggctgaa acttccgtcg cgggcggttt ggccggcttc tatgccgagt gcgcgcttga
     2101 cggcttccag tatattcgcc gagcctttga cgtggcaggc tgtgccgcgg cagacctgaa
     2161 cgtgataacg gccgcggggc tgaaaccgga actggttata aaaagtggct acgccgtaaa
     2221 tcttgctcgt cggcagattc aattgtcggc ctatcgccgt gacagcctcg cgggacaaat
     2281 atccctgggc atcctgtacg tcctgcagca gcggtatgag tttatcccgt cctgcgccgg
     2341 agtatttttc caaaatatcc tctattttca cagccatctt aaaattttcc ttgcttcgtc
     2401 aattttcggc tatcctcagc cgcccaacgc aggcagcgtc atttttttaa ttgcgtaaaa
     2461 taaatcaccc gctcaagcgc agtcatgatg tcaacgtgtt caacgtacgc cggcggattt
     2521 atcttcagat gaacgggtgc aaaattcaga aaacctctta tccccatctc gatgagcatg
     2581 tccgcgacag cctgcgctcc ggacgccggc acagcaagca tggctaccct gatgtcttcg
     2641 ctgtcgatta cttccttgag cttgtccatc gtgtagcagc gacagccgga aatgacccgg
     2701 ccggtaacgg cttcatcgcg gtcgaatgcc gccgcaatca ccagattgct ctgacgatcc
     2761 tgaaaataac tcagtaccgc cctgccgaga tggccgatgc cgaccaataa taccttgcgt
     2821 atctcagaac ctacgagtat ctcatccagg gcttcgagaa gtttttcgat attgtatccc
     2881 cgcgtcggcg taccctcggt tttgagcacc atcatatccc tgcgtacctg tgccggcgta
     2941 cagccggcca ggacggctaa ctgatgcgaa aacacgctcc gctggccgtc ttcccgcaga
     3001 ttcgcaagaa tgcgacgata taaactcaat cgacctatga ctctttccga taccattttt
     3061 ttaaattttt aaaacttttt aaaatttttt agactttttt agacttttta tatttttaca
     3121 cttttgaacc atatttaaaa aaatcgaaaa atctactgca ctctaccgca ctatttcgca
     3181 tcattcagcc acacgaattg caaaacatca gaatagttca caaaaatctt ttctttcgaa
     3241 aaccattcta aaattcagag catggcgtat atttttttat caatttcggt caaaatcagc
     3301 tataaaaacc atttcaataa aaaaccggtg tagtgccttc accttaaaat caagcctaca
     3361 agccggtata aatcgttgcg tttttcacaa tatctgtgaa aaacgcaaca ttgtgaatta
     3421 tttcacaggt agtattataa cttatcatta cccatatagt caaatatttt tatccgtgat
     3481 ttttttcacg gcgcggcagg tgcggttttc acgcgcggga tatgtttttc aataacattc
     3541 actccgacac tacgaggttt gaatgtgtag atatatgttc ctgactcagg aagtttcagg
     3601 cgaacaaagg gctgcccgtc ggcatcacag ccgatgaaat attctccagc cttcgaataa
     3661 ttgtccaacg cccaatttaa cagcgaatcc ggcagataat tttccttatc ctgactgacg
     3721 gcgagtttta atcggccgtc gtttgcatct ttgacaaacg tttcaaaatt tatgggctga
     3781 taccctttgg taaaatttct gccgataaag ctgataaata tccgcgcaca cgcaatgctg
     3841 tcgagttcag ccgtttcttc agccttcgtc aaccaccaat ttttatattc agcctcgatt
     3901 gtctcgacat tctccgcaaa gcagcttttc caagcttcgt cagccttcgt gccgcgggca
     3961 agctgacata aaaacttttc aaaattctcg cgatattttc catcatcggc atgcatgaaa
     4021 aaatgcacta tcgaccacgc ctggcagtag ttaaggttcg cttccgacct gaggccgttt
     4081 ttccattcct cgtcggaaat cgtcataaac tttttcatcg gggcgaaagc gttttttaga
     4141 agagcgtttc tgacaatcgc aagctgatta gccgacacga aaccttccag caattcatcg
     4201 cccgtccatt ttgcataatt gaaatattcc gccagtcctt cgttcagcca tagcggtatc
     4261 tgtggaaaag cgttcatggc gaactggtga aaaccttcgt gttgcatgac ttcaaaaacg
     4321 ctgccgttca gccacgccat cagccagact ttcggattgc gtttgcccat gcgagtccat
     4381 atataacatc cgcgtgtatt gggaaatccg ccggcggcat aataatcatt aacgtcggca
     4441 aacagataca cgttacatcg gcctggcatg gggcggtaaa gtttcacggc aagccgccgg
     4501 aaactgcgat aattacactc cagccggatg cgtgcaatca tggaatcgca aatatcccaa
     4561 tccgtataaa tcacataatg agtggttttg aacgggcgga atttgacggg cagggttttc
     4621 ctggtcggct cggtaaaatt ctcggcgaca gccgcatcaa ataaaacaac cagtaatata
     4681 actgccttta aaaaacgcat agccataatt tcaggaagta accggcacgc cgcagacgcg
     4741 ccacgacatc tttatttctt ctcagccgcc tcatgcacta tgtcagccag aacggtgttc
     4801 gccacaaaca gccgctctcg cggaatcatg agtttttctc cgttcatcgc cagcccgccg
     4861 gatgcaagat gcgtggaaat gctgtacgga aaagcgtctt caaaactcat gccgaacctg
     4921 tatccgaaat tcgtcagccg catgccctcg cgcatgcgca gacgaagcat gagagtctca
     4981 gccatggtca tgttcatatt gagcgtctcg gtttcaccag gcgaaggaat gctatcgtca
     5041 agcatcgcgc ttatccagcg tcgcacgtcg cgtatgttag tccgtcgcgt ggtaaaactg
     5101 tcgtgaatga aactgctggc ggcggacggg cctatcccta tatacgggta attgaaccaa
     5161 tacacgaggt tatgcctgca acgcaggccc ggccgcgcaa aattggaaat ttcgtaatgt
     5221 tcaaaacctg cgtttattag cgtatatatg gcggtgttgt acattctgag ctgttcttcg
     5281 tcttcaattt cctgcagttc gccgttggag agctgcgtgt gaaattccgt gccgttttca
     5341 tagctcaggg catagcaact gatatgttcc ggcgaaagcc gtacagcctg ccgcaggctt
     5401 tcccgccatt ttttgaggct ctggccgggc agtgcgaata tcaggtcgag atttatatta
     5461 tcaaaaccgg catcacgaag cattttgaac gaatcgaaaa tttcccgacg cgtatgtatt
     5521 cggccaatgc cggcaagctc atcgtcgtga aacgactgtg ctccaagcga cacgcgattt
     5581 atgttcatga accggcagag gcctattttt tcgttgtcaa gcgtgccggg attggcctct
     5641 atggtatatt ccgtgccagg gccgacgaat tttttaagat tgcccaaaag acgtcgcaaa
     5701 tgcggcatcg acaacgaggt aggagtgccg ccgccaacat atatcgtagc cagcggccga
     5761 gacagcatgc ccgtataaag ctgcaactcc ctctcaagag cctccacgta acggccgaca
     5821 agttcatcat ccaccgacac ggagtagaag tcgcaatatt tgcacttgcg cacgcaaaac
     5881 gggacatgaa tataaagtga gtcagctttt tttaacattg ttcagtacct gctggggggt
     5941 ctgtgcagcc tgccggtttt ttccggcaag aaaatcgttc agaaaacttg cggcagcgac
     6001 ggcatcctgt ctggccttgc gttttttcct ggtcatcgtg ccagccaacg tcatatccgc
     6061 atcaaaactg ctcagccgtt catcccacat tctcacgtcc aatccgccga cacagagttt
     6121 ttgggcgaac tccaacgttt tcatagctgc cggcccggcg gtatcgtcca tattcaaagc
     6181 caaccctaca atcacgacgc gacagtcata ttcctcgcag acatcggata ttttgcgtac
     6241 cagcgactcg tcgtcgccac cctgcaacac gcatagcggc gtggcaaaac cgtcggcgga
     6301 gtcgccgaca gccaggccca cccgcttatg gccgtaatca actccaaggt atcggggcat
     6361 ttttaaagaa atctcggatc gtggtcgcgt tcgattgcgt ctacaagttc cttgagacgc
     6421 t
//
LOCUS       DAONFI010000104         6396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14227_length_6396_cov_4.433843, whole genome shotgun sequence.
ACCESSION   DAONFI010000104 DAONFI010000000
VERSION     DAONFI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14227_length_6396_cov_4.433843"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..362)
                     /gene="rplW"
                     /locus_tag="PLK08_04565"
     CDS             complement(78..362)
                     /gene="rplW"
                     /locus_tag="PLK08_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPS52807.1"
                     /translation="MENDMFNTIIRPLVTEKGTHQSSSLNAYAFQVSPKANKAQIKQA
                     IEKIYNVKVVEVRTQSRKGKPRRTGRVMAHTEDWKKAIVVLHPDHHIDLF"
     gene            complement(365..988)
                     /gene="rplD"
                     /locus_tag="PLK08_04570"
     CDS             complement(365..988)
                     /gene="rplD"
                     /locus_tag="PLK08_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPS52808.1"
                     /translation="MFEVPVYNTQGKQVDTLQIGEETFGSSVNVDLVKQAVVTYHANR
                     RQGTVKTKSRGDVAFSSKKIYRQKGTGNARHGDKAAPILRKGGHTFAKAPRDFSKALP
                     RNMRRAALNSAILAKIIGQDIMVLEGLSCTEPKTGRMAELMKNLKVNRTCLLTLAERD
                     KNVYLSSRNIPDLMVRIAEELNAYDVATRSKMVVTREAMKLLCGKEA"
     gene            complement(1007..1663)
                     /gene="rplC"
                     /locus_tag="PLK08_04575"
     CDS             complement(1007..1663)
                     /gene="rplC"
                     /locus_tag="PLK08_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPS52809.1"
                     /translation="MLSALLGKKVGMTQVYDEAGVLHPVTVIQAGPCTVLQVKSSDSD
                     GYSAVQMGFEDVKPHRASKPEIGHAAKAGTAAKKFVREFRMDGDGDAEVGTTLTVEVF
                     DGVQYVDVIGTSKGKGYAGPMKRHGFGGFCATHGTERKHRAPGSIGGHGTNLGMGPKI
                     RKGKRMGGHMGCDRVSSRNHALVGIDRENNLLLIKGPVPGAKGGYLMIRKSKTAKVKA
                     "
     gene            complement(1682..1990)
                     /gene="rpsJ"
                     /locus_tag="PLK08_04580"
     CDS             complement(1682..1990)
                     /gene="rpsJ"
                     /locus_tag="PLK08_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPS52810.1"
                     /translation="MDNGRIRIRMEAYDHRALDSSAKEIVEHAERTGARVCGPIPLPT
                     RIERYTVLRSPHVDKKSRDQFEMRTHKRIIDIYEPTVRTVEALNRLVVPAGVFIKIKA
                     "
     gene            complement(2400..4472)
                     /gene="fusA"
                     /locus_tag="PLK08_04585"
     CDS             complement(2400..4472)
                     /gene="fusA"
                     /locus_tag="PLK08_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPS52811.1"
                     /translation="MAKEISKIRNIGICAHIDAGKTTVTERILFFAGRTYKIGEVHDG
                     TAVMDYLEEEQQRGITITSAATTFDWNGCCVNLIDTPGHVDFTIEVERSLRVLDGAIC
                     VFCGVGGVEAQSETVWHQADRYKVPRLCFINKMDRLGADFEMVVKEIETRLNGRPVKL
                     QIPMGASETFQGQIDLIRRKAYFYDPAETATTLRVEEIPAEYADEAELARTEMIDRVA
                     EFDDEVMEKYLAEEELGEQDILNAIRKGTLAGKIQPVLCGTALKHKGIRPLLDAVVDF
                     LPSPSDVPSIVGHESLDSDKKVIRHPDPKEPFSALVFKITSDTHGDLNFVRIYSGTLK
                     AGSRVLNATQDKKENVSRIWQMHAKQRYAREDASAGNIVALVGMRNCITGDTLCDPKH
                     PVVLERLAFPDPVISMSIEPRTNADKQELIDALNTLRREDPSFQYSYNSETGQTVISG
                     MGELHLEIVKNKLVRDMKLEVRVGKPRVAYKETATATKEIDHTFERQIGGRGLYARVM
                     LRVEPYEPAEGQPSVVFVNQCKEGISGQYLPAIKEGLLAASASGPLAGYPMQNLKITL
                     LGGQEHPVDSSEAAFTQAAAVAFNEAVQAANPVFLEPIMKLEVSTPDEFLGAITSDLA
                     SRRAIITGQESRGKYLVVTAEVPLSEMFGYSTQLRSLSQGRASGSMEPLKYAPAPPQV
                     GEKIFSFS"
     gene            complement(4581..4823)
                     /locus_tag="PLK08_04590"
     CDS             complement(4581..4823)
                     /locus_tag="PLK08_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52812.1"
                     /translation="MADENSLKETAADPDGLARSNRRGSSASRSRKSGRDKNSRLAGI
                     WCSTVTAGYAGSYPNRMHACEGVFTADTMTLPDGVD"
     gene            5300..6313
                     /locus_tag="PLK08_04595"
     CDS             5300..6313
                     /locus_tag="PLK08_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52813.1"
                     /translation="MKSGVFFLSMIFFCSTVLSDTVELGQKSYTDVLISGVTKDKILF
                     IQAGRVKTEKPLNEVTKLKIDLFPDFTRAEELAALNQYQKAMQQYLDAYTKVKSLKMT
                     WQLTSEECLDNGQKILGETYAANAKSEWIKDLIKLRYAKTKSMESTGGQQASAIPQEA
                     AQEPTDKPQTDAVKDETKTVNDNQICKECRGKGTVKCKKCNGSGKIKCTHKTITNGHD
                     EYHAHYYEICPTCDGKGFIPEKYQVWIPERYVATKYVPGYYSKRTRKVACPNPQCRPY
                     PKDPRIHIIWMCPLCATAKYPGYIDCPDCQNGRCTCPKCGGSGKITQETTPEQTNSRT
                     TQP"
BASE COUNT         1500 a         1690 c         1522 g         1684 t
ORIGIN      
        1 caccgacgaa ttacgccttc ctgctgatgt cggtttgtaa atttttatcg gcatggtggt
       61 ctaccttttc ttgctcttta gaacaggtcg atgtggtggt cgggatgaag caccacgata
      121 gcctttttcc aatcttcggt gtgtgccatc actcttccgg tgcgacgcgg tttgcccttg
      181 cggctctgcg tgcggacttc cacaaccttt acgttataaa tcttttctat cgcctgcttg
      241 atttgtgcct tgttcgcctt gggcgacacc tgaaacgcat aggcgttcaa cgaacttgac
      301 tgatgcgtgc ccttttcggt cacaagcgga cggatgattg tgttgaacat gtcgttttcc
      361 acggttatgc ctccttaccg caaagaagct tcatcgcctc gcgggtcaca accatcttgc
      421 tgcgcgtagc cacatcatag gcgttgagtt cttcggctat gcgtaccatg aggtcgggaa
      481 tgtttcgcga ggagagataa acgtttttgt cgcgttctgc cagcgtaagc aggcaggtgc
      541 ggttgacttt gaggtttttc atgagttcag ccatcctgcc cgttttgggt tcggtgcagc
      601 tgaggccttc gagaaccatg atatcctggc cgatgatttt ggccagaatc gcgctgttga
      661 gagccgcgcg acgcatattc ctgggcaggg ctttgctgaa atcgcgggga gctttggcga
      721 aggtatggcc gcctttgcga agaatcggag cggctttgtc gccgtggcgg gcgttgcccg
      781 tgcccttctg gcgataaatc ttcttcgaag aaaaagcaac atcgccacgg gacttggtct
      841 tcaccgtgcc ctgacgtcta ttggcgtgat acgtgaccac tgcctgcttc accaaatcga
      901 cgttgacgct gctgccgaag gtttcttcgc ctatctgcag cgtatcgacc tgtttgcctt
      961 gcgtgttgta tactggtact tcaaacatat ttcaatcctt tacgactcag gcctttacct
     1021 tggccgtttt ggacttgcga atcatgagat aaccgccttt ggcgccgggt accgggcctt
     1081 ttataaggag gaggttattc tccctgtcaa tacccacaag cgcgtgattg cggctggaga
     1141 cacggtcgca tcccatatgg ccgcccatgc gtttgccttt acgaattttc gggcccatgc
     1201 ccaggttcgt gccatggccg ccgattgaac ctggcgcccg atgcttgcgc tccgtgccgt
     1261 gcgtggcaca gaaaccgcca aagccgtgac gcttcatggg gccggcgtaa cctttgccct
     1321 tgctcgtccc gatgacgtcc acatactgaa cgccgtcaaa aacctcaacg gtcagagtcg
     1381 tgccgacttc agcatcgccg tcgccatcca tgcggaactc gcggacgaat ttcttcgccg
     1441 ctgtgcctgc cttggcggca tggccgattt caggcttgga agcccggtgc ggctttacgt
     1501 cctcgaagcc catctgaaca gcggaatagc cgtcgctgtc ggaactcttg acctgcagga
     1561 ccgtgcaagg tccagcctga atgacggtca ctgggtgaag taccccagcc tcgtcataca
     1621 cctgggtcat gccaactttc ttacctagta atgcagaaag catcattctc acctgccaaa
     1681 cttatgcctt aatctttatg aagacgcctg ccggtaccac gagacggttg agggcttcaa
     1741 ccgtgcggac ggtcggctcg tatatgtcga ttatccgctt gtgcgtacgc atctcgaact
     1801 ggtcgcggga cttcttgtcg acatgcggac tgcgaagaac cgtatagcgc tctatacgcg
     1861 ttggcagcgg gataggccca cacacgcggg ctcccgtgcg ctcggcatgc tctacgattt
     1921 ctttcgccga gctatccaac gcccggtgat cgtaggcttc cattcgaatc cttattctgc
     1981 cgttatccat ggagttttac tcctgctttc atctggtctt aactgtattt ccgcactctc
     2041 agcctggaga tgcagagccg taaaaattta cagattttcg gctttgtgtc aatcacaaaa
     2101 cctttatttt tcaatatttt tatcgaatcg tgacatcgtc acaaaaaact tccgcccata
     2161 cactaccgct gggccacgta ccactgcata ttgtggtctc acgcaaactg tcgtagctat
     2221 tttaaaaagt ttcaagttcc aggatggcaa cttacaagca atttttttaa taaagtatca
     2281 aggttcattt tcgtaaacat aacgccaatc gtcatatttt gtgaagttga cgcctgcgtg
     2341 aaattttttc ttgaacactc ttacttttta aactttttaa ctttttaaac aaattcagct
     2401 tagctgaatg aaaatatttt ttcgccaacc tgcggcggtg ccggggcata tttcagcggc
     2461 tccatcgacc cgctggctcg gccctggctc agtgaccgca actgcgtcga atatccgaac
     2521 atctccgata gcggaacttc agccgtcact acgagatatt ttcctctcga ctcctggccg
     2581 gtgattatcg cccgccgcga tgcaagatca ctggttatcg caccgaggaa ctcgtccggc
     2641 gtcgaaacct caagtttcat tatcggttca aggaaaactg ggttggccgc ctggactgct
     2701 tcgttgaacg ctaccgcagc tgcctgcgta aacgccgctt cacttgaatc aaccggatgc
     2761 tcctggccgc ccagcagagt tattttcaaa ttctgcatgg gataacccgc cagcggccca
     2821 cttgccgacg cggcaagcaa accttctttt atcgcaggca gatattggcc cgaaatgcct
     2881 tccttgcact ggtttacaaa aacaacgctc ggctggcctt ccgccggttc atacggctca
     2941 acgcgaagca taaccctcgc ataaagccct ctgccgccaa tctgacgctc gaaagtgtgg
     3001 tctatttcct ttgtcgcagt ggctgtttcc ttgtacgcca ctcgcggctt gccgacgcga
     3061 acttccagct tcatgtcgcg aaccagcttg ttcttgacga tttccaggtg cagctcgccc
     3121 atgccggata taaccgtctg gccggtctcg ctgttatagg aatactggaa actggggtct
     3181 tcgcggcgaa gcgtattcag cgcatctatc agttcctgtt tgtcggcgtt ggtacgcggt
     3241 tcgatggaca tggaaatcac cggatcggga aacgcaagcc gctcaagcac caccgggtgc
     3301 ttggggtcac ataacgtatc gccggttatg cagttacgca tgccgacgag cgccactata
     3361 ttgcctgccg acgcatcttc gcgggcgtat cgctgcttgg cgtgcatctg ccatatgcgg
     3421 ctgacgtttt cctttttatc ctgcgtggcg ttgagcaccc ggctgccggc tttgagcgtg
     3481 ccgctatata tgcggacaaa gttcaaatcg ccgtgcgtat ccgatgtgat tttgaacacc
     3541 agcgccgaaa acggttcttt cgggtcgggg tggcgaatca cttttttatc gctatcgagc
     3601 gattcgtggc ccactatcga cggaacatca cttggacttg gtaaaaaatc tacgaccgcg
     3661 tctagcagcg gcctgatgcc cttatgtttg agggctgttc cgcaaagtac aggctgaatt
     3721 tttccagcta gcgttccctt gcggatggcg ttcaaaatat cctgttcgcc gagctcttcc
     3781 tcggcgagat atttttccat aacttcgtcg tcgaattcgg cgactcggtc aatcatttcc
     3841 gtgcgagcca gttccgcttc gtcggcgtat tctgcgggaa tttcttccac tcgcagcgtg
     3901 gtagccgttt cagcggggtc atagaaatag gctttgcggc gaatcaggtc tatctggcct
     3961 tgaaaagttt cagacgcacc cataggtatc tgcaacttta ccggcctgcc gttaagcctt
     4021 gtctctattt cctttacaac catctcgaag tcagccccga gccggtccat cttgttaatg
     4081 aaacacaggc gaggcacttt atacctgtcc gcctgatgcc agaccgtctc gctctgggct
     4141 tccacgccgc ctacgccgca gaaaacgcaa atcgcgccgt ccagaactcg caagcttcgc
     4201 tcaacttcaa ttgtaaaatc aacgtgtccc ggcgtatcga tgagatttac gcaacatccg
     4261 ttccagtcaa aggtcgtagc agccgaagtt atcgtaattc cgcgttgctg ttcctcttcg
     4321 aggtaatcca tgaccgctgt gccgtcgtgc acctcgccga ttttgtaagt tctgccggca
     4381 aaaaacaata tgcgttcggt tacggtggtc ttaccggcgt ctatgtgtgc acagatgccg
     4441 atatttcgga ttttgcttat ctctttggcc atcactatcc ggtgtaacaa cacgccatca
     4501 tgcgattttc gtcgcactgt acaagcgagc gtccttaata agctaaaaaa actaaccgga
     4561 cgctcaaatt tcgccgacac tcaatcgacg ccgtcaggca gcgtcatcgt gtcagcggta
     4621 aaaacaccct cgcaggcgtg catccgattt ggataactgc ctgcgtaacc tgccgttacg
     4681 gttgaacacc atattccggc aagccggctg tttttatctc tgccgctttt gcgcgagcgg
     4741 cttgcgctcg agccccgacg gttactcctg gcgagtccat cagggtcagc cgcggtttcc
     4801 ttcaggctgt tttcgtctgc caagcctatg tattcagcct gccgacatct atcaccagag
     4861 gcgtttcgca aacctcttta agaaggacct ttcggccgat tcaaacattg ccgcttcgcg
     4921 gtttctttta aaattttttt tgagaaaaac cgcattggcc ttgtttgaag gaggtcaaat
     4981 aatatcagaa tcttttgtaa ccgcaagtgc tttatcgatt tttttaaaaa aatttcaaaa
     5041 tttttaaaat ttggattttt tacagcggcg ggtttagaaa tgctttgggg attttctttt
     5101 tatttttagt gtttaattcc taaattttaa tttgcatttc ccccaacatc tccttaacat
     5161 gaattttccc atggggtcag cagtcggggc cagcattttt acagccgtcg catgcaacct
     5221 caaggcaaaa aagcgtttgt atgtatagtc agaagcatcg aatttaatat tgccgaaaat
     5281 cttgagaata ggagacaaaa tgaaatccgg cgtatttttc ttatcaatga tatttttttg
     5341 cagcacagtt ttgagcgata ccgttgaact tggacaaaaa agttatacag atgttctcat
     5401 atcgggagtt accaaagaca agatactttt catacaggcc ggcagggtca aaacagaaaa
     5461 accgctcaat gaagtaacaa aattaaaaat agatttattt cctgatttta cgcgggcgga
     5521 ggaattggcg gcgttaaacc aatatcagaa ggctatgcaa caatatctgg atgcgtatac
     5581 gaaagtaaaa tcgctgaaaa tgacatggca gttaacatcg gaagaatgtc tcgacaacgg
     5641 acagaagatt ttgggcgaaa catatgccgc caacgcaaag agcgaatgga ttaaagacct
     5701 tataaaactc cgttatgcca aaaccaaatc aatggaatca acaggtgggc agcaggccag
     5761 cgccatccct caggaagcag cccaagaacc caccgataaa ccacaaactg acgccgtgaa
     5821 ggacgaaacc aaaactgtca acgataacca gatatgcaag gaatgtcgcg gcaaggggac
     5881 tgttaaatgt aagaaatgta acggctcagg taaaataaag tgtactcaca aaacgattac
     5941 caatggtcac gatgaatacc acgcacatta ttacgaaata tgtcctacct gtgacggcaa
     6001 gggttttatt ccggaaaaat accaagtatg gattcctgaa cgatacgtcg caacaaaata
     6061 tgtccccggt tactattcca agcgtacaag aaaagtggcc tgtccaaacc cgcagtgcag
     6121 gccttatccc aaagacccga gaattcatat tatctggatg tgtccgctat gtgcgacggc
     6181 aaaatacccc ggctatatcg actgtcccga ttgtcagaat ggccggtgca cctgccccaa
     6241 atgcggcgga agcggaaaga taactcagga aacaacgcct gagcagacaa acagccgtac
     6301 aacccaaccg taatattttg taaagtacgc gaatatgtat aaagatgtaa gaatatgaaa
     6361 accaggctac ttgtcgacta aacaaaacag cgaaaa
//
LOCUS       DAONFI010000105         6392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14241_length_6392_cov_4.263216, whole genome shotgun sequence.
ACCESSION   DAONFI010000105 DAONFI010000000
VERSION     DAONFI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14241_length_6392_cov_4.263216"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLK08_04600"
     CDS             complement(<1..768)
                     /locus_tag="PLK08_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52814.1"
                     /translation="MTLDEKIRQERATTRKLSRIYRFFTGAIFAILIAASLVVWIMQF
                     NSKVSQKKTDISQMLTDRCDALNNSLETAYYRVAATRDWLEDYLDYGYLSHGENMVKL
                     LHEEPGLGFYFCDSAGEPTTQGKDDDSKFWGGLYGFGSLEKLIKNQTRFTELDMAMHL
                     YELKRSQRQFMSRYFLWFLYISKNDFLTAGGLPSLTVDFHRAFMSSNVRPLMRKLYDN
                     RLYWKDILPESNPDRKIIWNRLDGIRASGLLVTISAPV"
     gene            939..1709
                     /locus_tag="PLK08_04605"
     CDS             939..1709
                     /locus_tag="PLK08_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52815.1"
                     /translation="MNLDIGDILNDWPYRPGEINVRRIIGKDGREKLQMRLDMGLLQM
                     ETVGRPDGRTPHGRKSLLDYYRELADNYARHHGSCLEFQLNEKDCEALRNEAVLYYHR
                     YLAEFVLADFPAVVRDTKHNLAIINFCNDFAEDDRDRLTLRQHVPYILMMNIRARARR
                     AIQASCPKRAAAVIEKGISIIEDFYRSTEQEEIIDLSAELKILKDLYNEIRVATPVSQ
                     EIRLEEKLRVALEEERYEEAAILRDKLRSLMHSKHRHK"
     gene            complement(1883..4024)
                     /locus_tag="PLK08_04610"
     CDS             complement(1883..4024)
                     /locus_tag="PLK08_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52816.1"
                     /translation="MKKSAVLFTMAGLLAVLLIDGCEELYAVKAMREKQSQVRSSRNV
                     TNSYQEEKNSPEARAEYKKNYTSKGAYKDSFLYEKETPLKAEKDLQEESGKSSTTQVK
                     SESAVAYSGPNEMQRLHDMRAAEEKQAVAAGKPIARKIERSDVGLTDPINVPKKQNAE
                     KIAPPAELLKPIIIDQQEEKTEARVSVPSKPKETKPVNNENVEAELKRLASKIENKPS
                     VVLPKEESEKTTATPAVQSESEPAIASAATQKTAEKIPATGGVDWNQLSAAQKAETPA
                     VVTTPAEPEMGKTPVSTAATTTVQNIKPTPTKTESTEAATAKIEAAPKTTANVTASAK
                     DEKSADSSWETEFVEKISSKVEKTETKSETSKPTGKNTDENLDWNLPKKELSKESVAE
                     ETPAAPAVNIDEPATVEIKLAQKFATEKTPEPAKPQTAKTVNSADEIKPKPITATQKA
                     PEKSITPAQVAVAAPKPAVPTMTDDEAAARIASENKRKVEIMARQVKILDNEKNYQEA
                     LTVLGKILEIDPANSWAKETETGIQAKIDASKKVTVSTTSAEPAVTNTKIVQTAPAVE
                     KAATTMVAAGTAPAPKAAPMAVTPKKPAVEPVVGANEDRTFKGWPGAGMILKSGMDRL
                     LSWLPENAAQFSNLRLMFSSNGGQTWKIVAEGLKQGRITAWTVPVVTSDNCRLKIVGS
                     DDGGEDVTLAISEQFSVNTGVWETVDSTAVK"
     gene            complement(4161..4952)
                     /gene="purQ"
                     /locus_tag="PLK08_04615"
     CDS             complement(4161..4952)
                     /gene="purQ"
                     /locus_tag="PLK08_04615"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPS52817.1"
                     /translation="MANPRALVLRTAGTNCDIETQFSLEQAGFSADRIHVNRIIENPA
                     MLDDYQFMLFPGGFSYGDDIASGKILASQMIHQLSEPLNRFYDAGKLMLGICNGFQIL
                     IKSGLLPWGRVDATTAHRDATLTWNDCGQFLCRWVHMRADSDKCVFMKKDEKISLPMA
                     HGEGKFVTTAGEILEKLKTQDQIALRYVNEDGSDASEAGVKAANPNGSTDNIAGICDP
                     TGRVMGLMPHPERFVFATQHPQWTRREINRADGKMFFDNAYEYLK"
     gene            complement(5201..>6392)
                     /locus_tag="PLK08_04620"
     CDS             complement(5201..>6392)
                     /locus_tag="PLK08_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPS52818.1"
                     /translation="VIKLYYEGASVGQMDMEFLHDGTPKPTKDALWDFKPMPTILPDV
                     LPDFNQILLKILAMPNVASKEWVIRQYDHEVQGGSAVKPLVGAAEDGPGDASVIRPKF
                     DSQKAVALSCGMNPLLGDLHPYQSALHAVDEALRNIVAVGGNLSRTALLDNFCWGNCN
                     KPDRMGTLVQSAKACHDAALAYGTPFVSGKDSLNNEFQTDKGETIAIPATLLISAMSV
                     LDDAGKCVTSDAKTAGNLLFIVGKTRDEMGGSHYLQAVGLKSGNDVPPVNCAANLESM
                     KKLQQAIEEGLVRACHDLSEGGLAVAAAEMAFSGELGVELSLADLPADGNVSLTAKLF
                     GESAGRFLVEVTPANEAAFTAVMSASAAKIGKVTDSDKIVIDNIIDLSAKSCKAAWQG
                     TFRW"
BASE COUNT         1308 a         1580 c         1652 g         1852 t
ORIGIN      
        1 aacaggcgcg gaaatggtta ccagcagccc tgaagctctt atgccgtcca gccgattcca
       61 tatgattttt ctgtcgggat tgctttcggg cagaatatcc ttccaataca gccggttgtc
      121 gtaaagtttc cgcatgagtg gcctgacgtt gctgctcatg aacgcgcgat gaaaatccac
      181 ggtcagagac ggcagaccgc cagccgtcag aaagtcgttt ttggaaatgt acagaaacca
      241 gagaaaatat cggctcatga actgacgctg gctgcgtttg agttcataga gatgcatcgc
      301 catgtcaagt tccgtaaagc gggtctggtt ttttatgagt ttttccagtg agccgaagcc
      361 gtacagtcct ccccagaatt tcgaatcgtc atctttgcct tgagtggtcg gttcgccggc
      421 tgagtcgcag aagtaaaatc caaggcctgg ttcttcatgc agcagtttga ccatattttc
      481 gccgtgcgag agatatccgt aatccagata atcctccagc cagtcacggg ttgccgctac
      541 cctgtagtaa gccgtttcaa gactgttgtt caatgcatcg cagcggtcgg tgagcatttg
      601 ggagatgtcg gtttttttct ggctgacttt gctgttgaac tgcattatcc agacaaccag
      661 gctagccgca ataagtatgg cgaaaattgc accggtgaaa aaccggtata tgcggcttaa
      721 ctttcgggtc gtagcccgtt cctgccggat tttttcatct aaagtcataa ttcagggtct
      781 gttacaaacc tatcgcctcc tgatgagcgt accagaaaat tatatcagat gcgacgcata
      841 ttttgtatat atcacaacga caaatatgat tttttttgtc gttaaacatt tttttctggc
      901 tgttgttttt tgatgaattg ggacgataaa atgtattgat gaatctggac ataggcgaca
      961 ttctgaatga ttggccgtac aggcctggcg aaatcaacgt ccgacgcata atcggcaagg
     1021 acggcaggga aaagctgcag atgcggctgg acatggggct tttgcagatg gagaccgtcg
     1081 gccggcctga cggcagaact ccgcacggcc gtaaaagcct gctggactat taccgcgaat
     1141 tagccgacaa ttatgcccga catcacggct catgcctgga attccagctg aatgaaaaag
     1201 actgcgaagc cctgcggaat gaagccgtcc tttattatca tcgttatctg gcggaattcg
     1261 tccttgccga ttttccagcc gtcgttcgtg atacaaaaca taacctggcg attataaatt
     1321 tttgcaacga ttttgcggaa gacgaccgcg accgtctgac tcttcggcag catgttccgt
     1381 atattttgat gatgaatatc agggcgaggg cgcggcgtgc gattcaggca agttgtccca
     1441 aacgggcggc agccgtcatt gaaaagggaa tttcgataat agaagatttt taccgttcga
     1501 cggaacagga agagataata gatctcagcg ccgaattgaa aatactcaag gacctgtata
     1561 atgaaatcag ggttgccacg cctgtttcac aggaaatacg gctagaggag aaactgcggg
     1621 tcgcgcttga agaagagcga tatgaagaag ccgccattct ccgcgacaaa cttcgctcac
     1681 tcatgcattc aaaacatcgg cataaataga tacccccgga caagccctga gcacttaccg
     1741 ttactgttgc taacacaact ggcccaagtc ttgctgcatg tacattattt cgtacaagca
     1801 tcagcattca tccacgagta tgctcgtggt attctgtaag gtctagaacc tatcccaaaa
     1861 accttggctg cctgaaatcg gcttatttta ctgccgtcga atccacggtt tcccacacgc
     1921 ctgtgttgac gctgaactgt tcgctgatgg ctaaggttac gtcttcgccg ccgtcgtcgc
     1981 tgccgacgat tttcaggcga cagttatcgc tggtgacgac gggtactgtc catgctgtta
     2041 ttcgtccctg tttcaagccc tcggcgacga ttttccacgt ctggccgcca ttgctgctga
     2101 acatcaaccg cagatttgag aactgcgccg cattttccgg cagccagctt agcagcctgt
     2161 ccatgccgct tttcaatatc atgccagctc ccggccaacc cttgaacgtt cggtcttcat
     2221 ttgcaccaac gaccggttca actgccggtt ttttaggcgt gactgccata ggagcagcct
     2281 tcggcgcagg agcagtcccc gccgcaacca tagtagttgc agctttttca accgccggag
     2341 cggtttgaac gatttttgta tttgtaactg caggttctgc tgaagtggtc gatactgtga
     2401 ctttcttcga ggcgtctatt ttggcctgaa taccagtttc cgtctctttt gcccatgaat
     2461 tagcagggtc aatttccaga atttttccga gtaccgtcaa tgcttcctga taattctttt
     2521 cattgtcgag aatcttgacc tgccgggcca ttatttcaac tttgcgtttg ttttcggacg
     2581 cgattcgggc ggcagcttcg tcatctgtca tcgtcggaac ggccggtttt ggtgcagcga
     2641 cagccacctg agccggcgtt atggattttt ccggcgcctt ttgagtagct gtaatcggct
     2701 tgggttttat ttcatctgcc gaattaaccg ttttggcggt ttgcggttta gccggttcag
     2761 gcgttttttc cgttgcaaat ttttgggcga gttttatttc tacggtagcc ggttcgtcga
     2821 tgttaaccgc tggagcggct ggcgtttctt cggcaactga ttctttggac aattctttct
     2881 taggcagatt ccaatcgaga ttttcgtcag tgttttttcc cgtcggcttg gacgtttctg
     2941 attttgtttc tgttttttct actttggacg atattttctc gacgaattct gtttcccacg
     3001 acgaatcggc ggatttttca tcttttgcgg aggcagttac attagcggtt gtttttgggg
     3061 cggcttcaat tttagccgtt gcggcttccg tagattcggt tttcgtaggc gttggcttta
     3121 tattctgcac ggtagttgtg gcagcagttg atactggcgt ttttcccatt tctggttcag
     3181 ccggcgtcgt gacgactgca ggcgtttccg ctttttgggc tgccgacagc tgattccagt
     3241 caactccgcc ggttgccgga attttttcgg cagtcttttg agtggcggct gacgctatgg
     3301 caggctctga ctccgattgt acggcaggag tcgcggtggt tttttctgat tcttctttgg
     3361 gcagaacgac ggatggtttg ttttctatct ttgaagcgag tcttttcaat tcagcttcga
     3421 cattttcatt attaaccggt ttggtttctt tgggtttgga cggaacagat acgcgggctt
     3481 cagttttttc ttcctgctgg tctatgataa tcggcttgag cagttcggct ggtggggcga
     3541 ttttttctgc gttctgtttt ttgggtacgt tgattgggtc ggtaaggcct acgtcgcttc
     3601 tttcaatttt cctggcgatg ggtttgccgg cagctacggc ctgtttttct tccgctgctc
     3661 gcatgtcgtg cagccgttgc atttcattgg gccctgaata ggcaactgcc gattcggact
     3721 tgacttgtgt tgttgaactt tttccggact cttcctgcaa atctttttcg gctttcaggg
     3781 gagtttcttt ttcataaagg aacgagtctt tataggcacc cttactggtg tagttttttt
     3841 tgtattccgc ccgggcttcc ggcgagtttt tttcttcctg atagctgttt gtcacatttc
     3901 tggaggaacg tacctgcgac tgtttttccc gcatggcttt gacggcatac aactcctcgc
     3961 agccgtcaat cagcagcaca gccaacaagc cggccatggt gaataaaact gcactttttt
     4021 tcatatgttc catctcctgt caaactgcca ggttacccgg cggtacgaat ttttttagaa
     4081 aaacctgttt aaacagtgta attcaggcat aaaaaaccgg atatattctt atattaaaaa
     4141 tttaaaaaat gtctggaaat ttattttaaa tattcatagg cgttatcaaa gaacattttg
     4201 ccgtctgcgc gattaatttc acggcgtgtc cattgcgggt gctgggtggc gaacacgaat
     4261 cgttcagggt gaggcataag gcccatgact ctgccggtcg ggtcgcatat gccggcgatg
     4321 ttgtccgtac tgccgttggg gttggcggct tttacgccgg cttcgctggc atcgctgccg
     4381 tcttcattta cgtatcgcag ggctatctgg tcctgcgttt tgagtttttc gagaatttca
     4441 ccggcagtcg tcacgaattt gccttcgccg tgagccatcg gcagggatat tttttcatct
     4501 tttttcataa aaacgcattt gtcgctgtca gcccgcatat gcacccatcg acagaggaat
     4561 tggccgcagt cgttccacgt gagggtggca tccctgtgtg ctgttgtcgc gtcaacacgg
     4621 ccccagggca ggaggcctga cttgataaga atctgaaatc cgttgcagat gcccagcatc
     4681 agtttgccgg cgtcgtaaaa tctgttgagc ggttcggaga gctggtgaat catctggctg
     4741 gccaggattt tgcccgacgc tatgtcgtcg ccgtagctga acccgccagg gaacaacatg
     4801 aattgataat catccagcat cgccggattt tctattatac ggttgacgtg tatgcggtcg
     4861 gcggaaaagc cggcctgctc aagcgaaaac tgcgtctcga tgtcgcaatt cgttcctgcc
     4921 gttctaagta ccaatgctct tggatttgcc atcactcgtt atccttccaa catctttgtt
     4981 gcattttgtc gaatcctccg ccggttgcga agaatttttt aaatttaatt ttcaaatcta
     5041 aaacttcaaa cagaagctaa ttgccaaatt ccaaatcttt atttagagct tcataccgat
     5101 aatcatgatt tccggcgtca tgcctgcgaa gtgtgggcat ggccccgata ctgacatttt
     5161 tcaaaagcac tttatcaact gtgattaccg gtcggctttc ttaccatctg aacgttccct
     5221 gccaggctgc tttgcaggat ttggcggaga ggtcgatgat gttgtcaatt acgattttgt
     5281 cgctgtcagt cactttgccg attttggcgg ctgaggcgct cattacggcg gtgaaggcgg
     5341 cttcgtttgc cggcgtgact tccacgagga atcggccggc tgattcgccg aacagtttag
     5401 ccgtcagact tacatttccg tcagccggca agtccgccag cgaaagttcc acgcccaatt
     5461 cgccggagaa cgccatttcc gccgccgcga cggctaatcc gccttcggag aggtcgtggc
     5521 atgcgcggac aaggccttct tcgatagcct gctgcaattt tttcatgctt tccaggtttg
     5581 cggcgcaatt taccggcggt acgtcgttgc cggatttcag gccgactgcc tgcaggtaat
     5641 gactgccgcc catctcgtcg cgcgtcttgc cgacgatgaa cagcagattg ccggcggttt
     5701 tggcgtcgct tgtgacgcat ttgccggcgt catccagcac gctcatcgcc gatatcagaa
     5761 gcgttgccgg tatggcgatg gtttcgccct tgtcggtctg gaactcgttg ttgagcgagt
     5821 ctttgccgga cacgaacggc gtgccgtaag ccagggctgc gtcgtgacat gctttggcgc
     5881 tctggacgag tgtgcccatg cggtcgggct tgttacagtt gccccagcag aagttgtcca
     5941 gcagggctgt acggctgaga ttgccgccga cggctactat atttcgcagt gcctcgtcaa
     6001 ctgcgtgcaa ggctgactga tacgggtgca ggtcgcccag cagcgggttc atgccgcacg
     6061 agagagcgac ggctttttga gagtcgaact tcggccggat aaccgacgcg tcgccagggc
     6121 cgtcttccgc ggcgcccaca agcggcttga cggcgctgcc gccctgaact tcatggtcat
     6181 actggcggat gacccactcc ttgcttgcga cgttcggcat cgccaggatt tttagcagta
     6241 tctggttaaa atcgggtaac acgtccggca gtatggtggg catgggtttg aagtcccaca
     6301 gggcgtcctt ggtcggcttg ggagtgccgt cgtgcagaaa ttccatatcc atctggccga
     6361 ccgacgcacc ttcgtaataa agtttgatga cc
//
LOCUS       DAONFI010000106         6376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14291_length_6376_cov_4.027844, whole genome shotgun sequence.
ACCESSION   DAONFI010000106 DAONFI010000000
VERSION     DAONFI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14291_length_6376_cov_4.027844"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLK08_04625"
     CDS             complement(<1..582)
                     /locus_tag="PLK08_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPS52819.1"
                     /translation="MTSGTTKCNQAYEYLRKQLLYCQLPPNMRLAETQWAPKLNVHRP
                     ALREAMTLLAHEGLLRRGDKGGFFTISLTLKQRRDILTTRSIIEAGAIRLYNSNPPTP
                     QQIEELSDACNLLKLLIEAKLDISIQEADRSFHEILVELSGNASLIHLYERSPLPFEG
                     PKQTDEYSMQKRLKTLADLQEVIRYGAVRRLRPK"
     gene            complement(787..1941)
                     /gene="galK"
                     /locus_tag="PLK08_04630"
     CDS             complement(787..1941)
                     /gene="galK"
                     /locus_tag="PLK08_04630"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPS52820.1"
                     /translation="MEIGELKSEFGKTFGCRPTGIAAAPGRVNLIGEHTDYNGGFVLP
                     IAIELRTAAAWSPRDDGRIVFRSMQQQAVCEIDLCGELSPGEPRWANYPKGVLAGLMR
                     QGIEPEGCNVLFTSSVPIGSGLSSSASLEVAAALVLMAAAEKRGEIGGIALAELCRQA
                     EHEYAGTPCGIMDQAISVLGRKDRALLLDCRDFSTKLIPFENTDVTLLVCDTQVKHSL
                     NDGGYAARRKCCEETAAILDVNTLRDATMPMLEDAFDAGRISETQFCRARHAVTEIAR
                     TVDAVKALQADDIKRFGRLMYGSHESLRDDYEVSCEELDIIVELAGGCDGVFGARMTG
                     GGFGGCAIVLAEASRADAVTKCIQDGFEKKFARRCPIFATRAADGANYLE"
     gene            2382..3020
                     /locus_tag="PLK08_04635"
     CDS             2382..3020
                     /locus_tag="PLK08_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPS52821.1"
                     /translation="MQPRIPVPTIRRLPGYLRLLREAAASRMDFISGTYLANELNLEP
                     IQVRKDLAITGVAGKPRTGFAVRELINAIESLLGWSNTHDAFLAGVGSLGIALMGYGG
                     FREYGLEIVAGFDIDKNKIGKTFHGKKVFAMEKLADLVQRLGICTCILTVPASAAQAV
                     TDRLIAAGIIGIWNFTPAKLQTPDNVIVEKVDLAASLAVLSSKLQARQAKLI"
     gene            complement(3070..3267)
                     /locus_tag="PLK08_04640"
     CDS             complement(3070..3267)
                     /locus_tag="PLK08_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52822.1"
                     /translation="MCKTFQSDAGASDETGMMLSTLAQRELEAAVGHMDKMMHSGDEA
                     DAFGHFIMMLTALKALRTELF"
     gene            complement(3504..3821)
                     /gene="rplU"
                     /locus_tag="PLK08_04645"
     CDS             complement(3504..3821)
                     /gene="rplU"
                     /locus_tag="PLK08_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPS52823.1"
                     /translation="MYAVIEDSGQQFRVSEGDVLEVDLRELAEDEKELKFERVLLVSD
                     DKGTRVGTPFVNGATVEAEIVDPCLKGEKVYHYHLRRRKASRRKTGHRQKYIRIRVTK
                     IKA"
     gene            complement(4024..5019)
                     /locus_tag="PLK08_04650"
     CDS             complement(4024..5019)
                     /locus_tag="PLK08_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPS52824.1"
                     /translation="MKNIGRTERMKISRVYRLLHIITLLQSGRSYSAAELARELCISR
                     RTVFRDLNSLELARIPYYFDLETKGFRISPHFFLQPLNLTLGEALSLLVMATNLRQES
                     SLPLLSEGAKAAAKIEGVLPVSVREYVGSIMNSLSVHLGPMASHDEADENFDLLSRAI
                     IKRFECRLNYESFYEGRRISVLVHPLRLMFIHRAWYLLAWSPAEKALRTYKLIRIHDL
                     ELRRRTFKNVRKNELENYFGMAWSMIPEGKIYRVHLHFSPKVAGNVAEVRWHRTQEVR
                     RNNDGSLEFFADVDGINEISWWVMGYGDQVKVVDPPELRRRIAEVADNVVKLYRQ"
     gene            complement(5024..>6376)
                     /locus_tag="PLK08_04655"
     CDS             complement(5024..>6376)
                     /locus_tag="PLK08_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HPS52825.1"
                     /translation="MAVCVGLWVYKFNGRVFIPSMIALVGIYFCIWLGTKLPVDLAKL
                     CGIPTLGGTYNSAVVIWTVILFIYCFLASILPVWLMLQPRDFINANQLFVALGLSIVG
                     VFAASLFGGADITASAPAIAKIIPEDAPPIWPFLFITVACGAVSGFHSLVSSGTSAKQ
                     LDNEYDALPVGYGGMLLEGALAVVVILACTAGTGMGLWDRNVDKNGVVKYTMITDAGG
                     NVIKNRQAWMTMYDASIHKKVDASGRTVVTGGWGGMKMQQQLEAFVEGASNFLRSIGI
                     PVEVAISLFATLVACFAATTIDTATRLDRYVIQELGAAIHFPPISNKYVATSIAVLSS
                     LGIAVFAGPKPGSGGMLLWPIFGACNQILAGLGFVLVAIYLLRRRINPWFVVIPLILM
                     VVLPAWAMCWNIFSDGGWLMEKNYLLVGFGIASMALTAWLLVEAGITYAAIKRQRPEG
                     "
BASE COUNT         1557 a         1776 c         1536 g         1507 t
ORIGIN      
        1 cttgggacgc agccggcgaa ccgccccata gcgaataact tcctggaggt cggcgagtgt
       61 tttgaggcgt ttttgcatgg aatattcgtc ggtctgctta ggcccttcaa aaggcagggg
      121 tgatcgctcg taaagatgta tcaggctggc gttgcctgaa agctcaacga gtatttcgtg
      181 aaagctgcgg tcagcttcct gtatgctgat gtcaagttta gcctctatga gcagtttgag
      241 caagttacac gcatctgata attcttcaat ctgttgcggc gtaggcggat tggaattgta
      301 aaggcgtatc gcaccagcct ctattatcga ccgcgtagtc agaatatccc gccgctgttt
      361 cagagtcagc gatatcgtaa aaaaaccgcc tttgtcaccg cgacgtaaca gaccctcatg
      421 ggcaagaagc gtcattgcct cccgcagggc aggcctgtga acgttaagtt tgggagccca
      481 ttgggtttcc gcaagccgca tattcggcgg aagttgacaa tacaataatt gctttcgcaa
      541 atactcatac gcctgattac atttggttgt tccactagtc atgagctata tgataccaac
      601 actgttaaca aaatcaatct atttctgttt tcaaaataaa ggttttttta gagccttgaa
      661 aaattgagag tatggctgat attggctagg tcatgaaata ttcgtgtatt tcttggcaaa
      721 aatttgccat aaattcgaat tttcgtccac tttgatattt tttttagcgt gcaaaacgct
      781 gcgtatttat tccagatagt ttgcgccgtc ggcggcacgg gttgcgaata tcggacagcg
      841 gcgtgcgaat tttttctcga aaccatcctg tatgcatttc gtaacggcgt cggcacggga
      901 agcctcagcc agcacaatag cacagccgcc gaatccaccg cccgtcattc ttgcaccaaa
      961 cacgccgtcg catccgccag ctaactctac aataatatcc agctcctcac aactgacctc
     1021 ataatcgtcg cgcagcgatt cgtgcgagcc gtacatcagc cggccgaacc gcttaatgtc
     1081 atcagcctgg agagccttta ctgcatcgac ggtgcgcgct atctccgtaa ccgcgtggcg
     1141 tgcacggcaa aattgcgtct cacttattct gccggcgtca aatgcgtctt caagcatggg
     1201 catcgtagca tcgcggagtg tgttgacgtc aaggattgca gcggtttctt cacagcattt
     1261 gcggcgagcg gcatatccgc cgtcgttaag cgagtgcttt acctgggtat cgcaaaccag
     1321 cagggtcaca tcggtatttt caaaaggtat gagttttgtc gaaaaatcac ggcagtccag
     1381 caatagtgcg cggtctttac ggcctaagac ggaaatggcc tgatccataa tgccgcacgg
     1441 cgtaccggca tattcatgtt cagcctgccg gcaaagctcc gcaagggcta tcccgccgat
     1501 ttcaccccgc ttttccgcgg cagccatcag tacaagcgcg gcagccacct caagcgacgc
     1561 cgacgaactc aggcctgagc ctatgggcac actgctagta aaaagtacat tacagccttc
     1621 cggctcaatg ccctgccgca tcaaacctgc taacacgcct tttggatagt tagcccatcg
     1681 cggctcgcct ggcgaaagtt cgccgcagag gtctatttcg caaaccgcct gctgctgcat
     1741 cgacctgaac actatccggc cgtcgtcacg tggcgaccag gcagctgccg tgcgaagctc
     1801 tatggctatc ggcagtacga atccgccgtt atagtccgtg tgttcgccga taagattcac
     1861 cctgcccggt gcagccgcaa ttccagttgg ccgacagcca aacgttttgc cgaactcgct
     1921 ctttaattcg ccgatttcca tggccgccga aggtatccgc acaaaacgtg cttgtcaaga
     1981 aaaaccggct aaaagccaca cgctcgcatt ttttattcac aaaagaaacc aagcggacga
     2041 aatcagcaga atcaccccta aaagaatcgt aaatacctcg gagatatccc agagaattga
     2101 atgatgtcct atcatctcaa gtacctccca aaaatgtcac cattgttttt atcgacattt
     2161 ttgtaacttc gtaataactg gcacttgtaa tttttattaa ataaacttat tattctccgg
     2221 aagctgtttc aacgcctgct ttcgtcgcga ggctgataaa tttagaggcc agccgcagca
     2281 gaaacaagat ttgacaacag cgcatctgct ccaaataaat ggattggttt ttgacttttt
     2341 gaggccgggc ctcagccggc ttcgtcagga ggcataaaag catgcagccg cgaatacctg
     2401 ttccgacgat tcgccggttg ccaggttatc ttcgcctgtt gcgcgaagca gctgcatcgc
     2461 gcatggactt catcagcggc acatacctgg ccaacgaact caaccttgag ccgatacagg
     2521 tcagaaaaga cctggcgata accggcgtag caggcaaacc ccgaacgggt tttgcagtcc
     2581 gtgagctaat taatgcaata gaatccctgc tcggctggag caatacgcac gatgcttttc
     2641 tggcgggtgt agggtcgctt gggatagcgt tgatggggta tggaggcttt cgcgagtatg
     2701 gcctggaaat agtggcaggc ttcgatatag acaaaaacaa aatcggcaaa acatttcacg
     2761 gcaaaaaagt tttcgccatg gaaaaattag ccgacctcgt tcagcggctg ggcatatgca
     2821 catgcattct gacagtgccg gcatcggcgg cacaggctgt aacggacagg ctcatcgcag
     2881 ccggcataat cggcatatgg aactttacgc cggctaaatt gcagacgccc gacaatgtga
     2941 tagtggaaaa agttgacctg gcagccagcc tggctgtgct gagcagtaaa ctccaggccc
     3001 gtcaggcaaa acttatctga tatttgcctg gaaaaaaaag gccggggagc gtaccggccc
     3061 gcggacacct cagaacagtt cagttctgag agccttcaat gcggtgagca tcatgataaa
     3121 atggccgaaa gcatcggctt catcgccgga atgcatcatt ttatccatgt gcccgacggc
     3181 agcctcaagc tcgcgctggg ccagcgtgga cagcatcatt ccggtttcat cggacgcacc
     3241 cgcatcgctt tgaaaagtct tacacattac accgctcctg taactgcgga acttccgcaa
     3301 ggtggtcgta tgtcagagca gttcctgaaa attacgaaag atttagaacc tatcccaaaa
     3361 accttggcta cccgcaaaat tgttcgattt cggcgacgcc catgtttggg gataggtttt
     3421 ataaaaaaag cccgccgtat ctgaacacag cgagcttgat agttctgaat tcatcagggc
     3481 tgcagccccg acatatctgc cggttacgcc ttgattttcg taacgcggat gcggatatat
     3541 ttctgacgat ggccggtctt gcggcgagag gctttcctgc gacgaagatg atagtgatac
     3601 accttttcgc cctttaaaca tgggtcgact atttcagctt caacggttgc gccgttcacg
     3661 aacggcgtgc cgacccttgt gcccttgtcg tcactgacga gaagtacccg ctcgaatttc
     3721 aattccttct catcttccgc cagttcccgc aggtcaactt ccaatacgtc gccttcgctt
     3781 acgcgaaact gttggccgct gtcttctatc actgcgtaca cttgtatctc cttgcattac
     3841 atgcagttaa agtcgcacat tcatccatcg tccttcggct ggaaggagcg gtaattatcg
     3901 tcaacagcct ttaattgtca agaattattt gccttttcaa taaaaaagtt gcaaaaacgt
     3961 cagccgccgg ctattgactc cacgacaggg ctgcgcggca tgtcgttaat ttcagaatcc
     4021 acgttactgc ctgtacagtt ttacaacgtt atcagccact tcggcgatgc gccgccgcag
     4081 ttccggcggg tcaacgacct ttacctggtc gccgtagccc atgacccacc agcttatttc
     4141 gttaattccg tccacatcgg caaaaaattc aagtgagccg tcgttattcc gccgcacttc
     4201 ctgcgtgcga tgccatctga cctccgccac attgccagcc actttcggcg agaaatgcag
     4261 atgcacgcgg taaatctttc cttccggtat catcgaccac gccatgccga aataattttc
     4321 cagctcgttt tttctgacgt ttttgaacgt cctgcgtcgc aattccaggt catgtatgcg
     4381 tatgagctta tacgtccgca atgctttttc cgccggcgac caggccagca gataccatgc
     4441 gcgatgtatg aacatcaatc gcagcggatg cactaagacg cttattcgac ggccttcgta
     4501 aaaactttcg tagttcaacc ggcattcgaa gcgtttgatt atggcccggc tgagaagatc
     4561 gaaattttcg tcggcttcgt catgcgatgc catcggccca agatgcacgc tcagggaatt
     4621 catgatgctg ccgacgtatt cccgcaccga cacgggcaat acgccctcaa ttttggctgc
     4681 agccttagcc ccttcagaca acagcggcag cgacgactcc tgccgcagat tagtggccat
     4741 taccagcaac gacagcgctt cgccgagcgt gagattcaac ggctgaagaa aaaaatgcgg
     4801 gcttatccga aagcccttcg tttccaggtc gaaataatac ggtatgcgtg cgagttccag
     4861 agaattcagg tcgcgaaaaa cggtacggcg gctgatgcac agttcacggg caagttccgc
     4921 ggcggaataa cttctgccgc tctgcagcag cgttattatg tgcagcaggc gataaaccct
     4981 gctgattttc atccgctcgg ttctgcctat gtttttcatc ggttcatcct tccggccgct
     5041 gtcgttttat cgccgcatac gtaattccgg cttcgacaag cagccacgcc gtcaaggcca
     5101 tcgacgcaat gccgaagcct accagcaggt aattcttttc catcagccag ccgccgtcgc
     5161 tgaagatatt ccaacacatt gcccacgccg gcagaacaac catcaatatc aacggtatta
     5221 ccacaaacca cggattaatg cgacggcgca ggagatatat cgccaccagc acgaatccaa
     5281 ggccggcgag tatctggttg catgcgccga atatcggcca gagcagcatg ccgccgctgc
     5341 cgggcttggg gccggcaaac acagctatgc caagactgct cagaaccgct atcgacgtgg
     5401 ccacatattt gtttgaaatc ggcggaaaat gaatcgccgc gccaagttcc tgaatgacgt
     5461 aacggtccag ccgcgtagcc gtatctatgg tagtcgccgc aaaacacgcc accagcgtgg
     5521 caaataacga aatggccacc tccacaggaa tgccgatact tctcagaaag ttggacgcgc
     5581 cttcgacgaa agcctccaac tgctgctgca ttttcatgcc gccccagccg cccgtcacga
     5641 cggttctgcc ggaagcgtca acttttttgt gaatgctcgc gtcatacatg gtcatccatg
     5701 cctggcgatt ttttatcacg ttgccgccgg catccgtaat catggtgtat tttacaacgc
     5761 cgtttttgtc gacgttcctg tcccacaatc ccatgccggt gccagccgtg caggctaaaa
     5821 tgacgaccac ggcaagtgcc ccttcaagca gcatcccgcc atagccgaca ggcagcgcat
     5881 cgtactcgtt atccaactgt ttcgcactcg tgccgcttga gaccagtgaa tgaaatccgc
     5941 tgacggcgcc gcaagcgacg gttatgaaca ggaacggcca tatcggcgga gcatcttccg
     6001 gaataatctt cgctatcgca ggagcggacg ccgttatgtc agctccgccg aacagcgacg
     6061 cggcaaacac acccacaatc gacagtccca gtgccacgaa caactggttg gcgtttatga
     6121 aatctcgcgg ctgaagcatc agccacaccg gcagaatcga cgcaagaaaa caatatatga
     6181 ataatatgac cgtccagatt accaccgcac tgttgtaagt gccgccgagc gtcggtatgc
     6241 cgcacagttt cgcgaggtcg acgggcaatt tcgtgcccag ccagatacag aaataaatcc
     6301 cgaccaacgc tatcatgctc ggtataaaaa ccctgccgtt gaatttgtat acccacaacc
     6361 ccacgcaaac agccat
//
LOCUS       DAONFI010000107         6292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14538_length_6292_cov_4.167549, whole genome shotgun sequence.
ACCESSION   DAONFI010000107 DAONFI010000000
VERSION     DAONFI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14538_length_6292_cov_4.167549"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLK08_04660"
     CDS             <1..408
                     /locus_tag="PLK08_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52826.1"
                     /translation="NLPKLPKIWLTAFIISSLVILYLRYHTDYLGQNIPLLECKYTSP
                     YVLLQAFFITCFFISLEYNNQKINDICSRYAPFTLGIYLAHPIIIYVIDAVTKNCKIN
                     SWILVPTMTIAVYFATLAMVLIFRKIPLLKAVV"
     gene            complement(418..1521)
                     /locus_tag="PLK08_04665"
     CDS             complement(418..1521)
                     /locus_tag="PLK08_04665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS52827.1"
                     /translation="MKITHISGSGKVGGIETHVMALSMAQRKLGHDVSIIIAQSAGPV
                     SEMLAAEGFDVRYANGTSGHDVMSMLRIRRIIRQINPDIVHLHGFSILSFISLAGIPF
                     VRVCTIHVLGRIRKSGLVRRFLSFVNNRIVRLYDGFIAVSADVRDRFLSDGCFTRTPW
                     CVIFNGIDESKFFPDDSLVLPTSADGQPIELLVVCRMEADKHPDDAIRVTDILRKKYG
                     MNVRLTLCGDGGILPACKKLTDELGISDFVTIAGRRNDVNEFMRKSHGLLFLSDYETF
                     GLTPIEAVASGCPVFCYRVSGGLHEWLKAEGCGGVISAKRTPESLAEVIATTLSNHEL
                     WQKLRNDAIACSSSFSSLEMAKKTLNYYRSLRG"
     gene            complement(1555..2934)
                     /locus_tag="PLK08_04670"
     CDS             complement(1555..2934)
                     /locus_tag="PLK08_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPS52828.1"
                     /translation="MSSLMALQDRLGRNIVFTLVIRVLGGADPKGTLIAALRDSGVKI
                     ADVELTEIEDSTNNLIYEITLFTTGDEQMASARKAADGIEGIEVVEVIDVAMEAHRGG
                     SCRMVAASPIESNTDLRIVYTPGVARVCKAIEADPSLADEYTGINNTVAIVTNGTAIL
                     GLGDIGCIAGMPVMEGKSAIFAEFAGISAIPMLVDTKDPEKFVETVCTIAKTFGAIQV
                     EDVAAPDCFYITSELDRRLDIPVMHDDQYGTATIVLAGLYSALKKTGKKIEALEVCIL
                     GAGAAGTAISDLLIKAGVKDVVLCDRYGAICKGRSNMNSAKQSLAGWTNKKNLQGDIA
                     AAIKGKDMFIGCSGPNLVSKDMIASMNDEPIVFALANPVSEISRDDALAGGAAIYADG
                     RTMNNAIAYPGIFRGALDAKSKVIDIPMMMAAAKALADIVPEGQLLPEMMDPKTHQTV
                     SQAVMKCVK"
     gene            complement(3216..3797)
                     /locus_tag="PLK08_04675"
     CDS             complement(3216..3797)
                     /locus_tag="PLK08_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPS52829.1"
                     /translation="MSEKSRHIFVSRAGEKLSAALDAFGINPAGWTCADFGANVGGFT
                     DCLLQRSAAKIYAIDTGYGDLAWKLRNDPRVVVMERTNALYVSPVDIVDMVVIDVAWT
                     PQKLAVPAAAKWLKSGGRIISLIKPHFELSKIKGRKPHQLLDEDEYELVLSSVRQNVA
                     DEGLCVAGVIPSPILGKGGNREYLMMVTTSDRF"
     gene            4052..5017
                     /locus_tag="PLK08_04680"
     CDS             4052..5017
                     /locus_tag="PLK08_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPS52830.1"
                     /translation="MPLIDMPLEQLKQYKGLNPKPADFDKYWAESIAEMKGVDPQIEI
                     ITADFPQTFAKCSDMYFTGVENGRLHAKLLQPLNPKPGKHPAIVAFHGYSGNVGQWTN
                     YACFAAAGYTVAALDCRGQGGSSNDATVAEGTNFRGHIVRGLADKNPKKLFFRRLFLD
                     AAQLAGIVMNMPDVDPTRVGAIGGSQGGGLTLACASLEPRIKRAAPAFPFLCDYRRVW
                     EMDLAKDAYEELQYFFRMFDPLHKNEDEIFTKLGYIDNQFLAPRIKAQIMMAVGLMDT
                     ICPPSTQFAAYNMITSQKSLEVYPDFGHEALPGWDDKVFEFMMGL"
     gene            complement(5279..>6292)
                     /locus_tag="PLK08_04685"
     CDS             complement(5279..>6292)
                     /locus_tag="PLK08_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPS52831.1"
                     /translation="VALAVVINSMLRPIFVATNVAQKVSQGDLREKPQITGSGEAAVL
                     LHSIRDMTDNLGSVVRQVQKTGVQVGASATEIFASTSQLEATTFQQVQFADKVLETSK
                     KISDTSSQLATTMSGVSSIAEKARHSAETSADYLERMEQTITQMEAASTAISQKLSRI
                     NEKAGNINEIIKTINQVAEQTNLLSLNAAIEAEKAGEYGLGFAVVAREIRKLADQTSS
                     RVRDIRRVIEEMQSAVSTGVMEMDKFTQEVTHGVKEAGIVGEKLGEIIEQVQEITPKF
                     EMVNTGMQDQSLGAVEIADAVSRLHEGARQINQALSEFKNVTQQLKDEASNLRDEIAK
                     FKV"
BASE COUNT         1608 a         1628 c         1415 g         1641 t
ORIGIN      
        1 aatctgccaa aattacccaa aatctggcta acggctttca tcatatcttc attagttatt
       61 ctttatttga gatatcacac ggattacctc ggtcaaaaca taccactcct tgaatgcaaa
      121 tacacgtcgc cgtacgttct gttgcaggca ttcttcataa catgtttttt catctcgctc
      181 gaatataaca atcagaaaat caacgacatt tgcagccgtt atgccccgtt tacattagga
      241 atatatctgg ctcaccctat cataatatat gtaatagatg ccgttaccaa aaactgcaaa
      301 ataaattcgt ggatacttgt gccaaccatg acgattgcag tctactttgc aactttggcg
      361 atggttctga tattcagaaa aataccacta ctgaaagccg tcgtataaca agaaatatca
      421 gcctcgcaaa gaccgataat aattcaatgt tttttttgcc atttccagtg atgagaaaga
      481 tgatgagcag gcaatagcat cattgcgaag tttttgccat agttcatggt ttgacaaggt
      541 cgttgcaata acctcagcca acgattccgg cgtacgtttg gcagaaatga ctcctccgca
      601 accttcggct ttcagccatt cgtgaagtcc tccactgacc ctgtaacaaa aaaccggaca
      661 gccgctcgca acagcttcaa taggcgtcaa gccgaatgtt tcataatccg acagaaataa
      721 taatccatgt gattttcgca taaattcatt aacgtcgtta cgacggcctg cgattgtaac
      781 aaaatccgat atgccaagtt cgtcggtaag ttttttacac gccggcagaa taccgccgtc
      841 cccacatagc gtaaggcgta cattcatccc atactttttt ctcagaatat cggtcactct
      901 tattgcgtcg tccggatgct tgtctgcctc catgcgacag accacgagca attctattgg
      961 ctggccgtct gccgaagtag gcaataccaa gctatcatca ggaaaaaatt tcgattcgtc
     1021 gatgccgttg aaaatcacgc accacggagt acgggtaaaa catccgtcag acagaaatct
     1081 gtctcgcaca tcggctgaaa ctgcgataaa cccatcatac aatcgaacaa tccgattgtt
     1141 cacaaacgac aaaaatcgtc taaccagccc tgattttcta attctgccga gaacatgaat
     1201 cgtacacact ctgacgaacg gtattccggc cagggaaata aatgataata ttgaaaagcc
     1261 gtgcaaatga acaatatcag gattaatctg tcggataatt ctacgtattc tcagcattga
     1321 catcacgtca tgcccgcttg ttccatttgc ataacgtacg tcaaaaccct cagccgccag
     1381 catctcacta acaggccctg cggactgggc aataataatc gaaacatcgt ggccaagttt
     1441 gcgctgtgcc atactcagcg ccatcacatg cgtctctatg ccaccgacct tgcccgaccc
     1501 tgaaatatgt gtaattttca ttttttccat tcggcgtaca tatctactga ttttttattt
     1561 gacgcatttc atgacagcct gcgacacagt ctgatgtgtt ttcgggtcca tcatctcagg
     1621 caggagttgg ccttccggaa cgatatcagc caacgctttg gcggcagcca tcatcatcgg
     1681 gatatctatc accttcgatt tcgcatccag cgcaccgcgg aagattccag gatacgcgat
     1741 ggcattgttc attgttctgc cgtcggcgta gatggctgca ccgccggcta atgcatcatc
     1801 tcttgatatc tcgctgactg ggttagccaa cgcaaacact atcggctcgt cgttcatgga
     1861 ggctatcata tccttgctca ccaggttcgg cccactacag ccgatgaaca tgtccttacc
     1921 tttaatcgcg gcggcaatat cgccttgcag attcttttta ttagtccatc cggccagaga
     1981 ctgcttggct gagttcatat tactgcggcc tttgcatatc gcgccatagc ggtcgcagag
     2041 aaccacgtct ttcacgccgg cttttataag caggtcggaa atggctgttc cagctgctcc
     2101 tgcgccaaga atgcaaactt ccagtgcttc gattttctta cctgtcttct tgagtgcgct
     2161 atacaagccg gcaagcacta tcgtggctgt gccgtactgg tcgtcatgca tcaccggtat
     2221 atccagacgc ctgtcaagtt cgctggtgat gtaaaaacaa tcgggggcgg ctacgtcttc
     2281 aacctgaatg gcgccgaaag ttttggcgat agtgcagact gtttcgacga atttctcagg
     2341 gtctttcgta tcgacgagca tcggtatcgc acttatgccg gcgaactcgg cgaaaatcgc
     2401 gcttttgcct tccataaccg gcatgccggc tatgcagccg atgtcgccga gtcccaatat
     2461 cgccgtgcca ttggtcacaa tggcgacggt gttgtttatg ccggtatatt cgtcagccag
     2521 cgatgggtcg gcttcaatag ccttgcacac gcgggcaaca cctggcgtat agacaattcg
     2581 caggtcggtg ttggactcta tcggcgaggc ggcaaccatt ctgcatgatc caccgcggtg
     2641 ggcttccatt gctacgtcaa taacctcgac aacctcgatt ccctcgatac catcagccgc
     2701 tttgcgtgcg gaagccatct gttcgtcgcc agtagtaaag agggttattt cataaatgag
     2761 gttattggtg gagtcctcga tttcagtaag ctccacgtcg gcaattttga cgccgctgtc
     2821 tcgcaatgcg gcaattagcg tacccttcgg gtctgccccg cccagcacgc gaatcacgag
     2881 cgtaaatact atattccgtc ccaacctgtc ctgcagcgcc attaatgatg acataaaact
     2941 tccttactga atttttaaac caaaaccaac tccgataacc agtacgagat tataacaact
     3001 accatcagga tgtcgagctt tacagacgaa tttgtatgaa acttaattaa aacctatcca
     3061 aaaaccttga ctgcctgcaa tcgactcttt tgcagcctgg ctgcgtcaga aaaacggcag
     3121 aatatgtttt tttaaaaagt atgctgccat ttttcttcct tgccagcccg caaaattgct
     3181 cgatttcggc gacgcccatg tttttgggat aggttctaga atctatcgct ggtagtaacc
     3241 atcatcaggt attccctgtt gccgcctttg ccgagaatcg gcgatggaat gacaccagcc
     3301 acgcaaagcc cttcgtctgc cacgttttgt ctgacgctgg aaagaaccaa ttcatattcg
     3361 tcttcgtcga gcagttgatg cggtttgcgg cctttaattt ttgaaagctc aaaatgcggt
     3421 tttatgagcg aaattattct gccgccggat tttaaccact ttgccgccgc cggcacagcc
     3481 agcttctgcg gcgtccaggc gacgtctatg acaaccatgt cgacaatatc gacaggtgaa
     3541 acgtaaagcg cattggtacg ttccatcaca acaacccgcg gatcgttgcg aagtttccag
     3601 gcaaggtcgc cgtaacctgt gtcgatggca taaattttcg cggcagaacg ttgcagcagg
     3661 caatcggtaa atccgcccac gttcgctccg aaatcagcgc aagtccaacc ggctggatta
     3721 ataccaaaag catccaatgc ggcggacaac ttttcaccag cgcgggaaac gaaaatatgt
     3781 cgtgattttt cactcataat gattgtcaca taatcaaaat tccaagccga ataacccgat
     3841 ggctgtccgg cattttacaa aagaacttcg tcatccatga aaactttttt gaaattttat
     3901 gaccaaaaga ttgaaaaaga cacttttccg tgataaaacc tcgcgcagtt gcatttttaa
     3961 aaatgtcctg agcggttctt catgattttt ttatgaaaat cgctttattt attatatctt
     4021 tttaaaaaaa caaaaattgg agaattgaaa aatgccgctt atagatatgc cactggaaca
     4081 gctcaagcaa tataaagggc tcaacccaaa accggctgat ttcgataaat actgggccga
     4141 atccatcgcc gagatgaaag gcgtcgaccc gcagatagaa ataatcactg ccgattttcc
     4201 acagactttc gccaagtgca gcgatatgta tttcacgggc gttgaaaacg gcagactcca
     4261 cgcaaaactg cttcagccgc tcaatcccaa gcctggcaaa catcctgcga tagtggcatt
     4321 tcacggatac agcggcaatg tcggccaatg gactaattat gcctgttttg cagcagccgg
     4381 ctatacggta gccgcgcttg actgccgcgg ccagggtggt tcttcgaatg acgctaccgt
     4441 tgcggaaggc acaaacttcc gcggccatat cgtgcgtggc cttgcagaca aaaatcccaa
     4501 aaaactcttc ttccggcggc tgttcctcga cgcggcacaa ctggccggaa tagtcatgaa
     4561 catgccggac gttgacccaa cccgcgttgg agctattggc ggcagccagg gcggcggact
     4621 cactctagcc tgcgcctcgc tagaaccgcg aatcaaacgg gcggctccgg cgtttccatt
     4681 cctatgcgac taccgcagag tatgggagat ggatctcgca aaagacgctt atgaagaact
     4741 gcaatatttc ttccgcatgt tcgatccgct gcacaagaat gaagacgaaa tcttcacaaa
     4801 gctcggctat atcgataacc agtttcttgc gccgcggata aaagcccaaa tcatgatggc
     4861 agtcggcctt atggacacca tctgtccgcc atcgacacag ttcgcggcat acaacatgat
     4921 aacgtctcaa aagtcgcttg aagtttatcc ggacttcgga cacgaagcct tgccgggttg
     4981 ggatgacaaa gtctttgaat tcatgatggg cctgtaatag aagctagagc attttaaact
     5041 tgaaatgcga cacttttatg cgttggcgaa acgagctatg caaggaaggc gaagcaggaa
     5101 tactgcccgt attccgatgc agtctgacgc agcagagcga agttgcagcc agcagaaatg
     5161 gtgcatatca agtttaaaat gctctaaagt aacacaaaaa acgaagtctc attaatagca
     5221 tttaaagtgg ttctcaaaaa ataagaaaat tttgagaacc actttaattc gccggttttc
     5281 acaccttgaa tttggcgatt tcatcccgca ggtttgacgc ttcgtccttc aactgctgcg
     5341 tgacgttctt gaattcgctc aaagcctggt taatctgtct ggcgccttca tgcagccggc
     5401 tgacggcatc ggcaatttca acagcgccaa gcgattgatc ctgcatgccg gtgttcacca
     5461 tttcgaattt tggcgttatt tcctgaacct gctcgatgat ttcgccaagt ttttcgccga
     5521 cgatgccggc ttccttgacg ccgtgagtga cttcctgcgt gaacttgtcc atttccatga
     5581 cgccggtaga gactgcggac tgcatttcct ctattacgcg acgaatatct cgcacgcggg
     5641 atgacgtctg gtcggcaagt ttgcgaattt ccctggcgac gacagcaaaa cccaagccgt
     5701 attcgccggc tttttcggct tcgatggcgg cattcaggct aagcaggtta gtctgttcgg
     5761 caacctggtt tatggttttt ataatctcgt tgatattgcc ggctttttca tttatgcggg
     5821 atagcttctg cgatatcgcc gtagaggcag cttccatctg ggttatggtc tgctccatgc
     5881 gttcaagata atcggcgctg gtctccgccg agtgtcgggc cttttctgcg atggacgaca
     5941 cgccggacat ggtagtcgcc agttgcgacg aagtgtcgct gatttttttg gacgtttcaa
     6001 gtaccttgtc ggcaaactga acctgttgga aagtggtcgc ttcaagctgg ctggttgacg
     6061 caaatatctc cgtcgccgag gcaccaacct gaacgccggt tttctgaacc tgtcgcacaa
     6121 ccgagccgag attgtcggtc atgtcgcgga tggagtgcag aagaacggcg gcttctccag
     6181 aaccggttat ctgcggcttt tcacggaggt cgccttgaga caccttctgg gcgacattcg
     6241 tagcaacgaa tatcggccga agcatggaat ttatcacaac cgccagtgca ac
//
LOCUS       DAONFI010000108         6278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14571_length_6278_cov_3.744336, whole genome shotgun sequence.
ACCESSION   DAONFI010000108 DAONFI010000000
VERSION     DAONFI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14571_length_6278_cov_3.744336"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLK08_04690"
     CDS             <1..871
                     /locus_tag="PLK08_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52832.1"
                     /translation="ELAGQFNVQELKINNLRQMLRLPPIEGVTLKPFVMTDISSHKLT
                     YSDGIFATPKFSAGVGDGKFVLDDFRVAAVPNEPLEITGDVTLQKIPIEPFYSAFDPT
                     QKVDFGWAYGWLKNICYIENNLNSLKMNGELFFDNSDLQDINVISQIHSQMKADTKKL
                     KNGSDLYMLFDIHDGYAYVQKGRLGNNLLAIHVLNGSKANITTQEVDLQVVVAMLDDI
                     RKVPVVGWLIQMNDALSKLQVSGKWNKPQVKPILVDKIDRGTREFFTGVAKTGGQIFF
                     PFADKNKKNGNAK"
     gene            complement(1051..2277)
                     /gene="metK"
                     /locus_tag="PLK08_04695"
     CDS             complement(1051..2277)
                     /gene="metK"
                     /locus_tag="PLK08_04695"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPS52833.1"
                     /translation="MAKKADSYLFTSESVTMGHPDKVSDMISDGIVDALLKYDPKARV
                     ACETLVTTGLVVLSGEITVHNVKAEQELHRAEEIARDTIKRIGYTDPEIGFDYRSCAV
                     IRSLHSQSADISQGVTETTSLDGDQGAGDQGLMFGYACNETKALMPLPIHLAHRLTAR
                     LTEARETGEIPWLRPDGKSQVTIEYRDGKPYKVNTVVISTQHTEAACGPDGNMSPKAK
                     KTIIEKIIKPVVMSECPALWSNKITFHINPTGRFIIGGPHGDTGLTGRKLIVDTYGGM
                     GRHGGGAFSGKDPSKVDRSAAYMARYIAKNVVAAKLADRCEIQLAYAIGVANPVSVHV
                     NCFGTNVIDTDLIADAICKIFPLTPKGIIKHLDLLKPKYQETAHDGHFGRTGKGFTWE
                     KTDMAKKLRTMLLKTK"
     gene            2564..2962
                     /locus_tag="PLK08_04700"
     CDS             2564..2962
                     /locus_tag="PLK08_04700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPS52834.1"
                     /translation="MSESTTSIRVRYCETDQMGTFYNARALDWFEVGRTELLRNSGIS
                     YAEIEKQGIMLPLVQAHVNFMGRARYDDKLLIVTTVKPDGKASLRFDCHIRHEDTGKA
                     VADGYTVHAVTDTVGKPTRPPKWMLELFNN"
     gene            complement(2983..3627)
                     /gene="purN"
                     /locus_tag="PLK08_04705"
     CDS             complement(2983..3627)
                     /gene="purN"
                     /locus_tag="PLK08_04705"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPS52835.1"
                     /translation="MMMANNGRKIRLGVLLSGGGRTLLNILDRIDAGTLDAEVAVVIA
                     SRPCEGISRCTARGLRVVMVAYRDFGPEKLQQYSDAIIEKLDAAEVDMVILAGFLSKF
                     VVPDRYIGRVMNIHPALLPSFGGKGFYGHHVHEAVVKTGVKVSGCTVHFVTNEYDAGP
                     IIVQRTVPVFDTDTPDDVASRVFEQECIAYPEAIELFAQGRLSIDGKIVRVKKM"
     gene            complement(3633..4361)
                     /locus_tag="PLK08_04710"
     CDS             complement(3633..4361)
                     /locus_tag="PLK08_04710"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HPS52836.1"
                     /translation="MATGLRIVATVLMMTGVAAVFPGCSHPEESGFLVNYNGFKETGL
                     LHKNLIYVNDGVDFTQYNAVKILPVRTYLMSTTDGVAPAEIDQLTDFFQQQLVDQFAV
                     YFRETNRSAQGVVIVRAAVTSLVPTDVAENAAAQGAGFIPGVGPVVSAGEAAYKGVTH
                     DTLGMGQASFEVEFLDAVTGQRIFGYVGMKEGSSLDIKGQATRWGVVETIMKKWATFL
                     AVKLKKLSDPSFDEGMVKKIEQQL"
     gene            complement(4402..5958)
                     /gene="guaA"
                     /locus_tag="PLK08_04715"
     CDS             complement(4402..5958)
                     /gene="guaA"
                     /locus_tag="PLK08_04715"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPS52837.1"
                     /translation="MAKAGQSEVVVILDFGSQYAQLIARRVREKNVYSILARPDLPIA
                     EIKKLNPKGIIFTGGPSSVYEPGAPRCDEKIFELGVPVLGICYGMQISSQILGAEITP
                     AERREYGRAKLHIKTDCSLLEGIPAETTVWMSHGDQVKDPGGDFEILAQTHTCPFAAV
                     RHKTIPFYGVQFHPEVTHTPHGEEIFDNFLYKICKCQGLWQMGSFIESVITDVREKVG
                     ENENVICGLSGGVDSSVVAALLHKAIGSRLHCIFVDNGLLRMNEIEMVESTFRDHFHI
                     NLVTVHGENDFLDKLRGVTDPQQKRKIIGHTFIDMFKAEAKKIPNAKFLAQGTLYPDV
                     IESGHSLAGQAANIKLHHNVGGLPEQLGFSLIEPLRDLFKDEVRRVGELLGLPENMVW
                     RHPFPGPGLAVRCLGEVTREKLDVLRKADNILLEEIHANNLYRQTSQTFAVLLPVQSV
                     GVMGDGRTYEDVVAIRSVNTTDFMTADWSHLPYEVLARISNRIINEVRGVNRVVYDIS
                     SKPPATIEWE"
     gene            complement(6081..>6278)
                     /locus_tag="PLK08_04720"
     CDS             complement(6081..>6278)
                     /locus_tag="PLK08_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPS52838.1"
                     /translation="RRIVRVNCFVNSATGFTDQAMVANGASELLQELFGQAGRHTRCA
                     LGSTELPLDSPVEIDIIAECE"
BASE COUNT         1472 a         1644 c         1564 g         1598 t
ORIGIN      
        1 cgagcttgcc ggccaattta atgtgcagga acttaaaatc aacaacctcc gccagatgct
       61 ccgtctgccg ccgatagaag gcgtgacgct caaacctttc gtcatgaccg acatttcatc
      121 gcacaagctg acttacagcg acggcatttt cgcgacgccg aaattttccg caggagtcgg
      181 cgacggcaaa ttcgtgctcg atgatttcag agtggccgct gtgccgaatg aacctcttga
      241 aatcaccggc gacgtaactc tccagaaaat acctatcgag ccgttctatt ccgccttcga
      301 cccgacacag aaagttgatt tcgggtgggc gtacggctgg ctgaaaaaca tttgttacat
      361 tgagaacaat ttaaattcac tgaaaatgaa cggcgaactg ttttttgaca attccgacct
      421 gcaggatatt aacgtgattt cgcaaatcca tagccagatg aaagctgata caaaaaaact
      481 caagaacggc agtgacctgt atatgctttt cgacatacat gacggttatg cgtacgtcca
      541 gaaaggccga ctgggcaaca acctgctggc aatacacgtg ctgaacggca gtaaagccaa
      601 tatcaccacg caggaagtcg atttgcaggt cgtcgtcgcg atgctcgacg atatcagaaa
      661 agtacccgtc gtcggctggc tgatacagat gaacgatgcc ttgtcaaaat tacaggtaag
      721 cggcaaatgg aacaaaccgc aggtcaagcc gatactcgtg gataaaattg accgtggcac
      781 acgcgaattc tttaccggcg tcgcaaaaac gggcggccag attttcttcc cgtttgcaga
      841 taaaaataaa aaaaacggta acgcaaaata gcagttttta tcacgggatg ctataggatt
      901 ctcttaaaag aggcctgccc atgtttcagg gatggttcga ataaaaaagg cgaagtcgta
      961 aattacgact ccgccctttt tgatttaaaa cagtcctcaa aaaaatcttt ttgagaaccg
     1021 ttttaacttt ttttgttctt cattgaaatt ttacttcgtc ttaagcaaca tggtacgaag
     1081 cttcttggcc atgtcagtct tttcccaagt aaaacctttg ccggtgcggc cgaagtggcc
     1141 gtcatgggcg gtttcctggt atttgggctt gagcaggtcc agatgcttga tgataccctt
     1201 cggcgtcagc gggaaaatct tgcaaatcgc atctgcgatg aggtcggtat caattacgtt
     1261 cgtaccgaag cagttgacgt gaacgctgac cggattggcg acgccgatgg cgtaggccaa
     1321 ctgtatttcg caacggtcgg ctaatttggc tgctacgacg ttcttggcga tatagcgggc
     1381 catatatgcc gccgaacggt caacctttga cgggtctttg ccgctgaacg caccgccgcc
     1441 atgccggccc atgccgccgt aagtatccac gatgagcttc ctgccggtaa ggccggtgtc
     1501 gccatgcggc ccgcctatga tgaatcggcc ggtgggattg atgtggaacg ttatcttgtt
     1561 cgaccagaga gcggggcatt cgctcataac gacgggcttg attatttttt cgataatcgt
     1621 ctttttagcc ttcggcgaca tgttgccatc cgggccgcag gcggcttcgg tatgttgggt
     1681 tgaaatgaca acggtgttca ccttgtaagg cttgccgtcg cgatattcga tggttacctg
     1741 gcttttcccg tcgggccgca gccacggaat ctcgcctgtt tccctagcct cggtcagccg
     1801 agccgtcagg cgatgtgcca gatgtatcgg cagcggcatg agcgctttgg tctcgttgca
     1861 tgcgtagccg aacatcaagc cctggtcgcc ggcgccctgg tcgccgtcaa gacttgtggt
     1921 ttcagtaaca ccctgagaaa tgtcagccga ctggctgtgc agcgaccgaa tgacggcaca
     1981 gctgcggtag tcgaacccga tttccggatc ggtgtaaccg atgcgcttga tagtgtcgcg
     2041 cgctatttct tccgcacggt ggagttcctg ttcggctttg acgttatgta ccgtaatttc
     2101 gccggagaga acaaccaggc cggtcgttac cagcgtttca caggctacgc gggctttggg
     2161 gtcatacttg agcaacgcgt caacaatgcc gtccgatatc atgtcggata ccttgtcggg
     2221 gtgacccatt gtcaccgatt cgcttgtgaa cagataacta tcagctttct tggccatcat
     2281 gattcctttc aaagtttatt gcacgggatt tggacgccgc cggtcaactc tcaaccgact
     2341 cagccgcacg cccgttacaa cgagctaatt taaaattcct gcaatgcagg atttactcta
     2401 caaagtaatt tcgaaattgt accattttcc ggcatgtcaa ttcaatactt tatccaagtt
     2461 taggcgcgaa aaaacagcga tgacggataa atcgataaat ttttattttt cgcaacctga
     2521 aaaaaatttg accgctgcag gattgttgca tatcctgttt tgtatgtcag aatcaaccac
     2581 atcaatacgc gtaagatatt gtgaaaccga ccagatgggc actttttaca atgcccgtgc
     2641 cctggactgg tttgaagtcg gcagaacgga actgcttcgc aatagcggaa tttcctatgc
     2701 tgaaattgag aaacagggca taatgctgcc gctggtgcag gctcacgtga atttcatggg
     2761 cagggcaaga tatgacgaca aactgctcat cgtcaccacc gtaaagccgg acggcaaagc
     2821 cagccttcga ttcgactgcc atatccgtca cgaagatacc ggcaaagccg tcgccgacgg
     2881 ttataccgtt catgccgtga ccgacaccgt cggcaaaccc acccgtccgc cgaagtggat
     2941 gctggaactt tttaataatt aataaataga gcagtgcgga tatcacatct ttttgactcg
     3001 cacgattttg ccgtctattg atagtcggcc ttgtgcgaaa agctcgatag cttccggata
     3061 ggcgatgcat tcctgttcga aaacgcggct ggctacgtca tcgggcgtgt cagtatcaaa
     3121 aacggggact gtccgctgta cgattatagg cccggcgtcg tattcgttgg tcacgaagtg
     3181 cacggtacag cctgagactt ttactccggt cttcacgacg gcttcgtgga cgtgatgccc
     3241 gtaaaaccct ttgccgccga acgacggcag cagggcggga tgaatgttca ttactctgcc
     3301 gatatatctg tcgggcacga cgaatttgga taaaaatccg gctaatatta ccatgtctac
     3361 ttctgcggca tcgagttttt cgattatggc gtcagagtat tgttgcaatt tttccggccc
     3421 gaaatccctg taagccacca taaccaccct caggcctctg gccgtacagc ggctgatacc
     3481 ctcgcatggt ctggatgcga ttacgacggc tacttccgca tccagcgtgc cggcgtcgat
     3541 acggtcgaga atatttagaa gcgttcgtcc gccgccggac agcaggacgc caagccgtat
     3601 ttttcttccg ttattggcca tcatcatcca ttctagagct gctgttcaat ctttttaacc
     3661 ataccctcgt caaatgacgg gtcggagagt tttttgagtt tgacggcgag gaaggtcgcc
     3721 cattttttca taatggtttc cacgacgccc catcttgtgg cttggccttt gatgtccagg
     3781 ctcgagcctt ctttcatgcc gacgtaaccg aaaatccgct ggccggtgac ggcgtcgagg
     3841 aattccactt caaaactggc ctggcccata ccgagtgtgt catgcgtcac gcctttatat
     3901 gctgcttcac ccgcacttac caccgggccg acgcccggaa taaatcctgc gccttgagcg
     3961 gctgcatttt cagccacgtc ggtcggcaca agtgacgtaa cggctgcacg tactattacc
     4021 acaccctgcg ccgagcggtt tgtctcgcgg aaatagactg caaactggtc gacaagctgc
     4081 tgctggaaga agtcggtcaa ctggtcaatt tccgcagggg ccacgccgtc ggtggtgctc
     4141 ataagatatg tacgcaccgg cagaattttt accgcgttat actgcgtgaa atccacgccg
     4201 tcatttacgt aaataagatt cttgtgaagc aggccggttt ccttgaagcc gttgtaattc
     4261 acaagaaatc cgctctcttc aggatggctg catcctggga aaaccgccgc aacacccgtc
     4321 atcatcagaa cagtcgcaac aattctcaat ccggtagcca ttttgatgcc tcctgttatt
     4381 ctgtatttta tcttaatcct gtcattccca ctctatcgta gccggcggct tggacgagat
     4441 gtcatatacg accctgttta cgccgcgaac ctcgtttatt atgcgattcg atatcctggc
     4501 cagcacttcg tacggcagat gcgaccagtc ggcagtcatg aaatccgtgg tgtttactga
     4561 gcggattgcg accacgtctt catacgttct gccgtcgccc atgacgccaa ccgactgcac
     4621 gggcagaagg actgcgaatg tctgcgaagt ctgccggtaa aggttgttgg cgtgtatttc
     4681 ctcaagcagg atattgtccg cttttcgcaa aacgtcgagt ttttcgcgag tcacttcgcc
     4741 caggcagcgt acggcaaggc cgggccctgg gaaggggtgt cgccagacca tgttttccgg
     4801 caggccgaga agttcaccga cccgtcggac ttcatccttg aagaggtcgc gaagcggttc
     4861 tataaggctg aaccccagct gttccggcag gccgcccacg ttatgatgca gcttgatgtt
     4921 cgccgcctgg ccggctaacg aatggcccga ttcgatgacg tccggatata acgtcccctg
     4981 agccagaaac ttcgcattgg gaattttttt cgcctctgcc ttgaacatgt ctataaatgt
     5041 atggccgatg attttacgct tctgctgcgg gtcggttacg cctcgcaact tgtcaagaaa
     5101 atcattttcg ccgtgaacgg tcacaaggtt gatgtgaaaa tggtcgcgga aagtgctttc
     5161 gaccatttca atttcgttca tccgcagcag gccgttgtcg acgaaaatac aatgcagccg
     5221 actgccgata gccttgtgaa gcagggcggc taccacggat gagtccactc cgcccgacaa
     5281 gccgcaaatg acattttcat tctccccgac tttttcgcga acgtcggtta tgacggattc
     5341 gataaacgaa cccatctgcc agaggccctg gcatttgcag attttgtata aaaaattgtc
     5401 gaaaatttct tcgccgtgcg gcgtatgcgt cacttcggga tgaaattgta cgccgtagaa
     5461 tggtatggtc ttgtgcctta cggcggcaaa tgggcaggtg tgcgtctgcg cgagaatttc
     5521 aaaatcgccg ccagggtctt ttacctgatc gccgtggctc atccatacgg tcgtttcggc
     5581 gggaatgccc tccagcaggc tgcagtctgt tttgatgtgc aatttggcgc ggccatattc
     5641 gcggcgttct gccggcgtta tttcagcacc gagtatctgg ctggaaatct gcatcccata
     5701 acagattcca agtaccggca cgcccagttc gaatattttt tcgtcgcatc gcggtgcgcc
     5761 tggttcatat acactcgacg gcccgccggt aaatattatg cccttgggat tgagcttttt
     5821 tatctcggcg attggcaaat ccggccgagc cagtatgctg tagacgtttt tctcacgcac
     5881 gcggcgagct atgagctgag cgtactgcga gccgaaatcc aatataacca ctacttcaga
     5941 ttgtcctgcc ttggccatgc ttaaaatatc caaaattttt taaaaacgat taaaatatta
     6001 aactgtttaa aactatatta aaaacatcca aaattttaaa aatcaaaaca tacgcactcg
     6061 atatgtcttt ccattttcca tcattcgcat tccgcgatga tatctatttc caccggcgag
     6121 tccagcggca attcggtaga gcccagagcg catcgcgtat gtctgccggc ttggccgaaa
     6181 agctcctgca gtaactcgct ggcgccgtta gccaccatag cttggtcggt aaagcctgtc
     6241 gcggaattca caaaacagtt cacgcgcact atacgacg
//
LOCUS       DAONFI010000109         6228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14739_length_6228_cov_3.375830, whole genome shotgun sequence.
ACCESSION   DAONFI010000109 DAONFI010000000
VERSION     DAONFI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14739_length_6228_cov_3.375830"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLK08_04725"
     CDS             <1..223
                     /locus_tag="PLK08_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPS52839.1"
                     /translation="HELATRLIARGFNSMFLWALEKNTACRFYERLGGKKSASRIVKF
                     GSADLVEIAYGWNNLHALAESTAEKQNPV"
     gene            complement(345..1397)
                     /locus_tag="PLK08_04730"
     CDS             complement(345..1397)
                     /locus_tag="PLK08_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52840.1"
                     /translation="MKKVILGLMLLGVVSGSSFAQIMGMDVDDSKMKVKILKTGADPK
                     YQLKQDFPAGKYRMTVDMNIKQSMVSDKQQPMPMNVSMKFIVLADISDYDKDGVKSLK
                     FSYESVEMVTPMGSGKAVRGQKPAADKTATSQPAGGNMMQMQAEQLVEIEGITVKMDK
                     TGKIISIEGVENMPGGQADMIANLVKDVSTSQKYPAVGVGGAFDVSAEAEMPMMGSML
                     IDTVTTLVSVDKTDKGNIGKLQSEITMSLKKDAAAAQKDDAAKKDEKAAPSTKPADAD
                     AEDEDENGGDVNAPKLDMQMNMKGAFTTTMDLATGLAIESVGTMTNDMTVQAGPQGAM
                     KMHQVIEMKMTTEPVK"
     gene            complement(1623..2627)
                     /gene="ilvC"
                     /locus_tag="PLK08_04735"
     CDS             complement(1623..2627)
                     /gene="ilvC"
                     /locus_tag="PLK08_04735"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPS52841.1"
                     /translation="MKIYYDKDGSLEPLKGKTVAVFGYGSQGHAHANNLRDSGIKVIV
                     SELPGTHNYNLAKEAGFEIYPAAEAAKKGDLLIITLPDELQAKIYKSDIAPNLAKGKT
                     LGFCHGFNIRFGQIVAPEGVNVIMIAPKGPGHTVRSCFVEGSGVPCLIAIEKDAGDAK
                     KIALAWGIGIGGGRAGILETTFTEETETDLFGEQAVLCGGLTSLVKAGFETLTEAGYQ
                     PHIAYFEVLHELLLIIKLMTEGGMDYMRYSISNTAEWGDLYVGPKIVDAHVKENMKKA
                     LKDIQSGKFAKEWLEECAKGKPNFKALYEKDRNHPIEVVGKELRKMFSWMEAKEVPAD
                     "
     gene            2966..3841
                     /locus_tag="PLK08_04740"
     CDS             2966..3841
                     /locus_tag="PLK08_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS52842.1"
                     /translation="MNASESPIWSAIQLLTSDNSDLSGCTNPAGDGSENFPRSRKTFS
                     KICERHAYLEVALPVSGKACIEMNGRWHVLSPGRFAAITPGVEHVEGCASARDRYTLL
                     WLTFSRHTMIAFACNHKPNNGWHTFMNCSLNSPHVSLLQELMQNGGTDFTPMRIKKIR
                     VNILAVLSELYAKQLDESSKQHKTAGCDKYKEVLESLNEFINTHYADHLNVDYLARMA
                     GVSTCYLNRLFKREFGAGVQEQIILAKMAAAKKMLKSGNFMIKQIAAKCGYDDPLYFS
                     KAFRKFYGYSPSSLL"
     gene            complement(3817..4653)
                     /locus_tag="PLK08_04745"
     CDS             complement(3817..4653)
                     /locus_tag="PLK08_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPS52843.1"
                     /translation="MAEKLDKIRNFLLDMDGTLYLGGRLFEATPAFLAAIHDSGRKLL
                     FLTNNSSRSTFEYHQKLNDMGLAACEDEIFTSTRATMIYLKNHTVYRKLYLLAVPTVE
                     EEFRQNGFDVTDESPDAVVLCFDRTLTYDKLRKACELIMRGTPYIATHPDLVCPTENL
                     PIPDAGSIIELIRAATGGAIPKIIGKPNSEMIDSAMEKLAATPADTAIVGDRIYTDME
                     MGFRAGLTTVLVLSGEAKREHLDSLARQPDYVVESVGDLTEILRSCAGFRKKLYKSDD
                     GE"
     gene            4956..>6228
                     /locus_tag="PLK08_04750"
     CDS             4956..>6228
                     /locus_tag="PLK08_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit E"
                     /protein_id="HPS52844.1"
                     /translation="MNGKILQRYEELACTARQFMEKRDLANKIVIQVGSATCENAAGA
                     KEVFSEFKKHVEASGRSDIAIHKVGCTGRCSCEPIVNVIVPGKFPVKYQCVDRELVHR
                     IFTQHILGGTPLEDAILDKSGSPLVKYDLLFCTSDRCRKNFPGDIIEALNEQLAAVGL
                     NNGEVIASQASCFGLCRRETSDQPSFMLVRPAKIVYRLCGREDLAKIVREHLRDGKIV
                     EELRVKDEPIAQRFFDLFGDVAFFNKQSRIALRNSGIIDPESIEEYIHCHGFEALARV
                     LDKNNPDWVINEVTESKLRGRGGGGYPTGKKWALGRASAGDVKYNICNADEGDPGAFM
                     DRSMLEGDPMSVIEGMIIGAFAIGAHKGFVYIRAEYPMAIRRLELAIESARKHGLLGD
                     NIMGSDFSYDIEIRLGAGAFVCGEETALIHSI"
BASE COUNT         1553 a         1631 c         1481 g         1563 t
ORIGIN      
        1 ccatgaattg gccacgcgac ttattgcacg aggttttaac tcgatgtttc tatgggcact
       61 ggagaaaaac acggcgtgcc gtttttacga acgtctgggc ggcaaaaagt cagcttccag
      121 aatcgttaaa ttcggcagtg ccgaccttgt ggaaattgca tacggctgga acaatctgca
      181 cgcactcgca gaatcgaccg ccgaaaaaca aaaccctgtt taattccctg gaattcaaag
      241 aattctcgaa aatcactaaa aatataaaaa agcgtgacga gcttagaggc ccgccacgct
      301 tttttgaaat tttcagataa acttagagct tctaaaccgt ttggttattt taccggttcg
      361 gtcgtcatct tcatttcaat gacctgatgc attttcatcg caccctgcgg gccggcttgc
      421 acggtcatat cattagtcat agtaccgacg gattctatgg cgaggccagt cgccaaatcc
      481 atcgtagtgg tgaatgcacc cttcatgttc atctgcatat caagcttggg agcgttgaca
      541 tcgccgccgt tttcatcttc atcctcagca tccgcatctg cgggtttggt tgacggagcg
      601 gctttttcat ctttcttggc ggcatcgtct ttttgtgcag ccgcggcgtc tttcttcaga
      661 gacatggtta tctcagactg caatttgccg atgttaccct tgtcggtctt atcaacgctg
      721 acaagcgtag ttacggtatc tatgagcata ctgcccatca tgggcatttc ggcttccgcg
      781 gatacgtcaa acgcaccgcc gacgccgacg gctggatatt tctgcgacgt tgagacatcc
      841 ttgacaaggt tcgcaatcat atccgcctgg ccgcctggca tattttcaac accttcaatg
      901 ctgataatct tgccggtttt gtccattttg acagtaatgc cttcgatttc gacgagttgc
      961 tcagcctgca tctgcatcat attgccgcca gccggctgac tggttgcggt tttgtcggcg
     1021 gccggcttct gcccgcgaac agccttgccg ctgcccatag gcgtcaccat ttcaactgac
     1081 tcgtatgaga atttcaggct ttttacgccg tccttgtcat aatcagatat atccgccaga
     1141 acaatgaatt tcatgctgac gttcatcggc ataggctgct gtttatcact taccatggac
     1201 tgcttgatat tcatatccac ggtcattctg tacttaccag ctggaaaatc ctgcttgagc
     1261 tgatatttcg ggtctgcacc ggttttgagg attttgacct tcattttcga gtcatcgacg
     1321 tccataccca taatctgggc aaaagaactg ccgctcacca ctcccaggag catgagcccc
     1381 aaaatcacct ttttcatgtc gtatctcctt gttaaaagtt gatttatcat aacattaagc
     1441 cggtaatgcc gaaagcaaaa aacgactctt tacaattttt tccggatgtt gtcattgtag
     1501 acgcctaatc aggcaaaagc cacatttcgc cataaaaaaa cgtgatgcgg catttttttt
     1561 aaaaaaccgt atcacgtttt atttttacag tcgttagtca gaaagctcgc ctagccacac
     1621 acttagtcag ccggcacttc cttggcttcc atccaactga acattttgcg aagctctttg
     1681 ccgacaactt cgattgggtg gttgcggtct ttttcgtaga gagccttgaa gttcggctta
     1741 cccttggcac attcttccag ccattctttg gcgaatttgc cgctctgaat gtccttgaga
     1801 gccttcttca tattttcctt cacatgcgcg tccacaatct tcgggccgac gtacaggtcg
     1861 ccccattccg ccgtgtttga aatgctgtaa cgcatgtaat ccatgccgcc ttcagtcatc
     1921 agcttgatga tgagcagcag ttcgtgcaga acttcaaaat aggcgatatg cggctgatag
     1981 cctgcttcgg tcagagtttc aaagccggct ttcaccagcg aggtcaatcc gccgcaaagc
     2041 acagcctgtt cgccgaagag gtctgtttcc gtctcttccg taaacgtggt ttcgagaatg
     2101 ccagccctgc cgccgccaat gccaatgccc cacgcgaggg ctatcttctt ggcgtcgccg
     2161 gcgtcttttt ctatggctat caggcacggc acgccgctgc cttcaacaaa gcagctgcgg
     2221 actgtatggc ccgggccttt gggggctatc attatcacat tcacgccctc aggagcaact
     2281 atctggccga aacggatatt gaatccgtgg cagaagccca gagttttgcc cttggcgagg
     2341 ttgggagcta tgtcgctctt gtaaattttg gcttgcagtt catcggggag agtgatgata
     2401 agcaggtcgc ccttcttggc ggcttcggca gccggataga tttcaaaacc ggcttccttg
     2461 gccaggttgt aattgtgagt gccgggaagt tccgaaacga ttaccttaat tccgctgtca
     2521 cgcagattgt tggcatgtgc gtggccttgg ctgccgtatc cgaaaacggc aactgtttta
     2581 cctttgagcg gctcaagcga accgtccttg tcatagtaaa tcttcatttg tgactcctgt
     2641 catagaaaaa aatagtgtac gttaatctct attgctctgg aatttgtatc tgtcagcccc
     2701 aactaggcca gcataaccca aaacccgttg caagactgta ctcagtctca aaaaactacc
     2761 ggaaatcaga tttttaccca ttctgccccc tgccgtcaag aataatcacc gccgtcagtg
     2821 gagattgttt taatgcagaa ttctgaacgg ctgatatttt gaagcatttt tccggttttt
     2881 atgcattatt gacctcgact gcagaaaatg ccggcaaaat aaaaaacatg gaaaaattcg
     2941 tacatcggtc tataatcgat atcatatgaa tgcgtctgaa tcaccaatat ggtcagcaat
     3001 tcaattactc acatcggaca attccgattt gtcgggatgt accaatccag ccggcgacgg
     3061 cagcgaaaat tttccgcgca gccgtaagac tttttcaaaa atctgcgagc gacacgcata
     3121 tctggaagtg gcgttgccgg tatcgggcaa ggcgtgcata gaaatgaacg gcagatggca
     3181 tgtgctttcg ccaggacgat ttgccgcaat tacgccaggc gtggaacatg tggaaggctg
     3241 cgcgtcggct cgcgacagat atacgttgtt gtggctgact ttttccaggc atacgatgat
     3301 agcattcgca tgcaatcaca aaccaaataa cggctggcat accttcatga actgttcact
     3361 gaattcgcct cacgtcagcc ttttacagga gttgatgcag aacggcggaa cagatttcac
     3421 gcccatgcgc ataaagaaaa tccgcgtcaa tattctggcg gttctctccg agctttacgc
     3481 aaagcagctc gatgaatcgt caaagcagca caagacggcc ggctgcgaca aatacaaaga
     3541 agtcctcgaa tcgcttaacg aattcatcaa cacccattat gcagatcatc tgaacgttga
     3601 ctaccttgcc aggatggcag gcgtttcgac atgctattta aaccgccttt tcaaacggga
     3661 attcggcgca ggcgtgcagg aacagataat acttgcaaag atggcagccg cgaaaaaaat
     3721 gctcaaaagc ggcaatttca tgataaagca gattgccgcc aaatgcggct acgacgaccc
     3781 gttatacttc tcgaaggcgt tcagaaaatt ttacggctat tcgccgtcat cgcttttgta
     3841 aagtttttta cggaatccgg cgcagcttct gagtatttcc gtcaaatcac ccacagactc
     3901 gacgacgtaa tcaggctgtc gtgcaaggct gtccagatgt tcacgcttcg cctcgccgct
     3961 caataccagc accgtcgtaa ggccggcccg aaaccccatc tccatatccg tgtatatcct
     4021 gtcgccgacg atagcggtat cggctggcgt ggcggcgagt ttttccatcg ccgagtcaat
     4081 catctcgcta ttcggtttgc cgataatttt gggaatcgct ccgccggtgg cggctctgat
     4141 aagttcgata atcgaaccgg catccggtat cggcagattt tccgtcggac agactaaatc
     4201 cggatgcgta gcgatatacg gcgtaccgcg cataatcagc tcgcacgcct tgcggagctt
     4261 gtcataagtc agcgtcctgt cgaaacacaa taccaccgca tcgggcgatt cgtcggtcac
     4321 gtcaaaaccg ttctgacgaa attcttcttc aaccgtcggc acagccaaca gatacagttt
     4381 ccggtaaacg gtgtgatttt tcagataaat catcgtcgcc cgcgttgacg tgaaaatctc
     4441 atcttcgcac gcggccagcc ccatgtcgtt gagtttctga tgatattcaa acgtactgcg
     4501 ggacgaatta ttggtaagaa acagcagttt gcggccggaa tcgtgaatcg ccgccagaaa
     4561 agcgggcgta gcctcaaaca gacggccgcc aagatacagc gtaccatcca tatccaataa
     4621 aaaattgcgt attttatcca gtttttcagc cattattcag catgttttcc tgtttcgcct
     4681 ataacgccgc attccagccg caaagtgcgt aattttacgc ctttcgtata aatgttgcaa
     4741 ccataagaat acctaccgaa aacatactgc cggcgaaaca tcgcattaaa gtggttttcg
     4801 aaagaatttt ctttttgaga atcgttcaat tttaaaaaaa tcaaaaagcg gcacgaattt
     4861 gaatacatcg tcgcaagcgg ctacaattga cataagaagg ctgcacaggc ataatcggtg
     4921 tgctgcctca aatctgattc aaatgaaagg ctgccatgaa cggtaaaatc ctccaaaggt
     4981 acgaagaact ggcctgtaca gcccggcagt tcatggaaaa acgggatctc gcaaataaaa
     5041 tagtcatcca ggtcggctcg gcaacatgtg aaaacgccgc cggcgccaaa gaagtttttt
     5101 cggaattcaa gaaacacgtc gaagcctccg gccgcagcga catcgcaata cataaagtcg
     5161 gctgtaccgg ccgatgcagt tgcgaaccga ttgtaaacgt aatcgtaccc ggcaaatttc
     5221 ccgtcaagta tcagtgcgtc gacagggaac tcgttcatcg catattcacg cagcacatcc
     5281 tcggcggcac gccgcttgaa gatgccatcc tggacaaaag cggctcgccg cttgtgaagt
     5341 acgacctgct cttctgcacc agcgaccggt gcagaaagaa tttccccggc gacattatcg
     5401 aggcgctcaa tgaacagctc gcagcagtcg gcttgaacaa cggcgaagtc atcgccagcc
     5461 aggcaagctg tttcgggctc tgccgccgcg agacgtccga ccagccgtcg ttcatgctcg
     5521 ttcggccggc caagattgtc taccgtctgt gcggcaggga agacctcgca aaaatcgtgc
     5581 gggaacacct gcgggacgga aaaatagtcg aagaactccg cgtcaaggac gaaccgatag
     5641 cccaacgatt tttcgacctt ttcggcgacg tggcattttt caacaaacag tcccgcatag
     5701 cattgcgtaa cagcggcatc atcgaccccg aatcaataga agaatacatc cactgccacg
     5761 gatttgaggc gttagccagg gtattggaca aaaacaatcc cgactgggtc attaacgagg
     5821 ttacggagtc gaaacttcgc ggccgcggcg gcggcggata tcccacgggc aaaaaatggg
     5881 cgttgggccg cgcgtcagcc ggcgatgtga aatataacat ctgcaacgcc gacgaaggcg
     5941 accctggcgc atttatggac agaagcatgc tcgaaggcga cccgatgagc gtaatagagg
     6001 gcatgataat cggggcattc gccatcggcg cgcataaagg cttcgtttac atacgcgcgg
     6061 agtacccgat ggcaattcgc cggcttgagc ttgcgattga aagtgcccgc aaacacgggc
     6121 tgctcggcga taacatcatg ggcagcgatt tcagttacga tattgaaatc cgtctcggtg
     6181 caggcgcgtt cgtatgcggc gaggagacgg cgttgataca ttccatcg
//
LOCUS       DAONFI010000110         6222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14761_length_6222_cov_4.384790, whole genome shotgun sequence.
ACCESSION   DAONFI010000110 DAONFI010000000
VERSION     DAONFI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14761_length_6222_cov_4.384790"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLK08_04755"
     CDS             complement(<1..797)
                     /locus_tag="PLK08_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3826 domain-containing protein"
                     /protein_id="HPS52845.1"
                     /translation="MSKLKAIFAVSVLSVMCGFAMAQPAGGPDGSGGPGGRPQMTPPD
                     GGPDGPCEPQACEGGMESEFYKAVNATEEQIEKLKAVFRTQMEKMKSWREANKEKFQA
                     ARKSMQEARESKDKDAISKAKEQMKALGEEGKKIHEQFIKDLSAVLDDTQMTKAKEFF
                     KSKMDNRHMRGERDKGDRGGMLEQLGLTDDQKAKIKAIMEEARTKADAAENSEEKREI
                     MESARKKINDEVLTDEQKAKLEKMKPEKGQSRPGEYLAAMNPLEGIAT"
     gene            complement(1006..1818)
                     /locus_tag="PLK08_04760"
     CDS             complement(1006..1818)
                     /locus_tag="PLK08_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52846.1"
                     /translation="MPRKKYDEFSAGIFVILASAILFGVVLWVGSADLLSKNVGVATF
                     YSDLTNGPQGIINDSPIVVGDLKIGKVNYISYDASNKRVIYSAVITQPGYAVYADAQA
                     RVVSGLLGGSSIMITDFGTRNSGPAGWNKPISISGGMSEIMNNFAVITENLKQLVVTN
                     SENINELIDNMVSVSANLNAASKEIRRNPWKLLYKPDDKTLKYTNIYDAARSFDDAAT
                     RLNIVATKLKALEQLDSEDPQAKEKIKLMQEELQKTFEKFQGMEQVFWKEVE"
     gene            complement(1859..2737)
                     /locus_tag="PLK08_04765"
     CDS             complement(1859..2737)
                     /locus_tag="PLK08_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS52847.1"
                     /translation="MSSNSENMPVQQNPVPPVITVRDLVKRFDGRAVLDGVNLDVYPG
                     EMLVIMGGSGCGKSTLLRQIIGASQPDEGYVELFGQKLGDMNDDEFDDACKRFGILFQ
                     SGALFNSMTIGENVALPLREHTDLDEETIEIMVKIKLEQVGLRQAEYLMPAEISGGMK
                     KRAGLARATALDPEVLFYDEPSAGLDPVTSAEIDVLMMDMAQKLGVASVVVTHEMDSA
                     FRIADRMIMLDKGKVLMNDTRQAFEKIRDGEPTGDETTDLIRQFLRGDCEGPITRRRQ
                     SSGYEEDILQFAGDWR"
     gene            complement(2721..3551)
                     /locus_tag="PLK08_04770"
     CDS             complement(2721..3551)
                     /locus_tag="PLK08_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPS52848.1"
                     /translation="MSDENPKLSRFSVVRLIGARTLRLIGFVGGMSVLFMGAMYVILM
                     SAFSRKYHVDVAALARQVVRIAIRSLGIVCLVQIFIGVILALQLEPTLKDFGQESQIA
                     SIIGIGGFRMLGPIITAVVLSGFAGASIAAEIGTMVVSEEIEAMEAMALNPVRFLVVP
                     RLIATFLAMLLLTPIADLMIALGGYVTARLVLGPTVYSEYWQLMRRMLEYKDFWTGMI
                     QAGVFGILISLIACREGLLVHGGAEGVGKATTMTVVNSIVAIVAAAGVCTVIFYVFKL
                     "
     gene            complement(3555..3890)
                     /locus_tag="PLK08_04775"
     CDS             complement(3555..3890)
                     /locus_tag="PLK08_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS52849.1"
                     /translation="MSGVIKQTDWQDKTVLVVVGGDIDMHTSAELQKGFQEILKNKPE
                     RIIVNLTAVPYMDSSGVASLVKLLSTSRKSKIDLRLFGLSKRVKAVFDITRLTTVFNI
                     CNTQEEAMG"
     gene            complement(3890..4327)
                     /locus_tag="PLK08_04780"
     CDS             complement(3890..4327)
                     /locus_tag="PLK08_04780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPS52850.1"
                     /translation="MSPEKNNKLEICIKSDPAALGEVRRKIDDFVARVGLGDEEAGEI
                     VLSMDEALTNVIRHAYEGRTDRPIEISLIDDGETICVEIRDYGIQVPVGKIHSRDLGD
                     VRPGGLGVHIMHECMDCVKFLPQKNGGTLLVMKKCVKRHMGNK"
     gene            complement(4336..4812)
                     /locus_tag="PLK08_04785"
     CDS             complement(4336..4812)
                     /locus_tag="PLK08_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS52851.1"
                     /translation="MAGLLTEELGGFLGMISRLIVLLGIALIPVFLFTGCIASMPTGG
                     SVYAGLQSEDPAVRIQSAVDAGKNRDKKAIPLLVECLDDNESDVRMFAGMALKRIVGE
                     KTYDELGWRFYFTRTERRAAVERWREWVAKNCGDGRQNDWQKLDSLFKAEKGAVAG"
     gene            complement(4876..5352)
                     /gene="ispF"
                     /locus_tag="PLK08_04790"
     CDS             complement(4876..5352)
                     /gene="ispF"
                     /locus_tag="PLK08_04790"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPS52852.1"
                     /translation="MLRTGLGYDSHAFISGDGVMLGGVRIPCEYSFRAHSDGDVVLHA
                     LADAMLGALALGDIGEHFPDTDKRWKNADSSILLGHVVKLAQDRGFVPVNIDITIIAQ
                     KPQILPHKRQMIENIAYMTYLEQDFVSIKAKTNEHMGFIGRCEGVAVLATVLMEKL"
     gene            5738..>6222
                     /locus_tag="PLK08_04795"
     CDS             5738..>6222
                     /locus_tag="PLK08_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPS52853.1"
                     /translation="MDVYPLKFNPIYKEKIWGGRSFERLFHRELPPDTNIGESWELAD
                     LPEGVSIVSNGEYAGRTLSDITVSLGEKLLGQTPAMPDGRFPLLLKFLDAEDILSLQV
                     HPDKTAVAQIGGKAALKTECWYIVESREGYIYRGIIPGTTPAEFKQAIETDQAEKYVK
                     R"
BASE COUNT         1493 a         1694 c         1398 g         1637 t
ORIGIN      
        1 gtggcgatgc cctcaagcgg gttcatcgcc gccaggtact cccccggccg cgactggcct
       61 ttctcgggtt tcattttttc gagcttggct ttctgctcgt cagtcagaac ttcgtcgtta
      121 atcttcttgc gggcggattc catgatttca cgtttttctt cagaattttc ggcagcatcg
      181 gctttggtgc gtgcttcttc cataatagcc tttattttgg ctttctggtc gtcagtcaga
      241 ccaagctgtt caagcatgcc gccgcggtcg cctttatccc gttcgccacg catatgacga
      301 ttatccatct tactcttaaa gaactctttg gcttttgtca tctgggtatc atcaagaaca
      361 gccgacaaat ctttaataaa ctgttcatga atttttttgc cttcttcgcc aagagctttc
      421 atctgttctt tagccttgga aatggcgtcc ttatccttcg attcgcgagc ttcctgcata
      481 gatttgcggg cagcctggaa tttttccttg ttggcttcac gccatgattt catcttttcc
      541 atctgggtgc ggaaaacggc tttgagtttt tcaatctgtt cttcggtagc gttgacggct
      601 ttgtaaaatt ccgattccat tccgccttcg catgcctgcg gttcacacgg accatcaggg
      661 ccgccatccg gaggtgtcat ctgaggcctg ccgccagggc cgcctgaacc atcagggccg
      721 ccagccggct gagccatcgc aaaaccgcac atcacagaca aaacagacac agcgaaaatt
      781 gcttttaatt ttgacatttc aatactcctt acttttgtta tgttactgac aggattaacg
      841 aaaaatcatc aaaattattg cttttaaaat gaaaatccgg aaaagtctgc tgatacttct
      901 ccggattagg gtagttctca aaattcaatc agacaaactg ttatattcaa aaaaaatatt
      961 cagatagctg ttattccttt taaggcctct tgttgcattt agaacctatt cgacttcttt
     1021 ccagaaaacc tgttccatgc cctggaattt ttcgaacgtt ttctgaagtt cttcctgcat
     1081 gagttttatc ttttccttag cctgcgggtc ttccgaatcc aactgttcga gagccttgag
     1141 tttggtggca acaatattca gccgcgtggc tgcatcgtca aaactgcgag ccgcgtcgta
     1201 aatgttggta tatttcaggg ttttgtcgtc aggcttgtaa agcagcttcc acgggttgcg
     1261 acgtatttcc ttgctggcgg cattgaggtt agccgatacg gaaaccatgt tgtcgataag
     1321 ttcatttatg ttttcactgt tcgtgactac gagctgcttg aggttttccg ttatgacggc
     1381 aaaattgttc attatttcgc tcatgccgcc ggagatggat atcggtttgt tccagccggc
     1441 tgggccggaa ttcctagtgc cgaaatccgt tatcatgatg ctgctgccgc caagaaggcc
     1501 ggacaccaca cgcgcctgcg catcggcata gacggcgtag ccgggctggg ttatgacggc
     1561 actgtaaatc acccgcttgt ttgaagcatc gtagctgatg tagttaacct tgccgatttt
     1621 gaggtcgccg acgacaatcg gactgtcgtt gattatgccc tgcgggccat tggtaaggtc
     1681 ggaataaaac gtcgcaacgc cgacattttt ggacagcagg tcggcagaac caacccacag
     1741 aacaactccg aacaatatcg cgcttgccag tattacaaaa atacccgcac tgaattcgtc
     1801 atattttttt ctaggcatta tcctgtccct tataatgtcc gctcaattat tttctaaatc
     1861 atcgccagtc gccggcaaac tggaggatgt cttcttcata tccgctcgac tgccggcgtc
     1921 tggttattgg gccttcacaa tcgccgcgaa ggaactgccg tatcaggtca gtcgtttcat
     1981 cgccggtagg ctcgccgtca cgtatcttct cgaacgcctg ccgggtgtcg ttcatcaaca
     2041 ctttgccctt gtcaagcatt atcatacggt cggcgatgcg gaacgccgaa tccatctcat
     2101 gcgtcaccac cacgcttgcc acgccgagtt tctgcgccat atccatcatc agcacgtcaa
     2161 tctcagccga cgtgacaggg tcaagtccgg ctgagggttc atcgtaaaaa agcacttcag
     2221 ggtcaagcgc cgtcgcgcga gccaggccgg cacgtttctt catgccgccg gaaatctcag
     2281 ccggcataag atactcagcc tgacgcaatc ctacctgttc aagttttatc ttgaccatta
     2341 tttctatggt ttcttcgtca aggtctgtat gctctcgcaa cggcagtgcg acgttttcgc
     2401 cgatagtcat ggaattgaac aatgcgccgg actgaaacag tatgccgaag cgtttgcagg
     2461 cgtcgtcgaa ttcatcgtcg ttcatatcgc cgagtttttg tccgaaaagt tccacgtaac
     2521 cttcgtccgg ctgacttgcg cctattatct gccgcagcag cgtacttttg ccgcagccgc
     2581 tgccgcccat tatcaccagc atctcgccag gatatacgtc cagattcaca ccgtctagca
     2641 cagctctgcc gtcgaacctc tttacgaggt ctcgcactgt tatgaccggc ggaaccggat
     2701 tctgctgaac cggcatgttt tcagagtttg aagacataaa atatcaccgt acatacgcca
     2761 gccgctgcga caatggccac aatcgaattg accacggtca tggtggtggc tttgccgaca
     2821 ccctcggctc cgccgtgcac aagaaggcct tcccggcagg caatgaggct tatgagtatg
     2881 ccgaacacgc cagcctgtat catgccggtc cagaaatcct tgtattcgag catccgccgc
     2941 atcaactgcc aatattcgga ataaaccgtc ggcccaagca ccagcctggc tgtcacgtaa
     3001 ccgcccaacg ctatcatcag gtcggcaatg ggcgtaagca gaagcatcgc cagaaacgtg
     3061 gctatcagcc ggggaactac caaaaatcgc acaggattca gggccatcgc ttccatcgct
     3121 tctatttctt cggaaacgac catagtgccg atttcggcgg caatcgacgc gccggcaaag
     3181 ccgctgagca ccacggctgt aattatcggg ccaagcatcc tgaacccgcc tatcccgata
     3241 atggacgcta tctgggattc ctggccgaaa tccttcagcg tcggctcaag ctggagtgcc
     3301 agtattaccc cgatgaatat ctgtacgaga cagactatcc caagactcct gatggctatt
     3361 cgcacgactt gccgggccag cgcagcaacg tccacgtgat attttctgga aaacgcgctc
     3421 atcagtatga cgtacatcgc acccatgaac agcacgctca tgccgccgac gaaacctatc
     3481 agccgcaacg tcctggcgcc tatgagccgc acaacggaaa atcggcttaa tttcgggttc
     3541 tcatcgctca tggattatcc catcgcctct tcctgcgtat tacagatatt gaataccgtc
     3601 gtcagacgcg tgatatcgaa gacggctttg acccgcttgc taaggccaaa gagacgcaaa
     3661 tcgattttac tcttgcgcga agtgctgagc agtttaacca gcgacgctac gccggatgag
     3721 tccatgtacg gcacagcggt gagattgacg attatccgtt cgggtttgtt tttcaaaatc
     3781 tcctgaaaac ccttctgcag ttcggcactg gtgtgcatgt cgatatcgcc gccaacaaca
     3841 accagaaccg ttttgtcctg ccagtctgtc tgttttatca cgccgctcat tatttgtttc
     3901 ccatatgtcg tttaacacat tttttcatga ccagaagcgt gccgccgttt ttctgcggca
     3961 gaaatttcac gcaatccata cactcgtgca tgatgtgaac gcccagcccg ccaggccgca
     4021 catcgcccag gtcacgggaa tgaattttac caaccggcac ctgaatgccg tagtcccgta
     4081 tttcgacgca tattgtttcg ccgtcgtcaa taagcgatat ttcaatgggc ctgtcagtcc
     4141 tgccctcata ggcatgacga atcacattgg taagcgcctc atccatcgag aggactattt
     4201 cgccggcttc ttcgtcgcca aggccaaccc tggcgacgaa atcgtcaatt tttcgtcgca
     4261 cttcgcccaa tgccgcaggg tcggatttta tgcatatttc gagcttattg tttttttccg
     4321 gactcatttc agtttttagc cggcgacggc gcctttttca gccttgaaca acgaatcaag
     4381 tttctgccag tcattctgac ggccatcgcc gcaatttttc gcaacccatt cacgccaccg
     4441 ctcaaccgcc gcgcgacgct cggtacgggt aaaataaaac cgccagccaa gttcgtcgta
     4501 agtcttttcg ccgacgattc gcttgagagc catgccagca aacatgcgaa cgtcggattc
     4561 attgtcgtcg agacactcta cgagaagtgg aattgccttt ttatcgcggt ttttgccggc
     4621 atcaacggcg gactgtatgc ggacggccgg gtcttccgac tgcaagccgg cgtaaacgct
     4681 ccctcctgtc ggcatactag ctatgcagcc cgtaaacaga aaaacgggta tcaacgctat
     4741 gccaagcaga actatcaatc gcgatatcat acccaaaaag cctccgagtt cctcggttaa
     4801 caatccagcc attctgcgat attatatgcc acacaaaaac gacttacaac attttggcgg
     4861 ctattttggc attttttaca gtttttccat cagaaccgtc gccagaacag cgacaccctc
     4921 gcaacggcca ataaagccca tgtgctcatt ggtcttggcc tttatgctca caaaatcctg
     4981 ctccagatac gtcatatagg cgatattttc tatcatctgc cgtttatgcg gcagaatctg
     5041 gggtttttgg gcaattattg tgatatcgat attgacagga acgaaccccc tgtcctgcgc
     5101 cagcttgaca acatggccaa gcagtatgga actgtcggcg tttttccacc gcttatcggt
     5161 atcggggaaa tgttcaccga tatcgcccag cgccaacgct ccaagcatgg cgtcagccag
     5221 cgcatgaaga accacatcgc cgtcgctgtg ggcacgaaat gaatattcac agggaatcct
     5281 caccccgccg agcattacgc cgtcgccgga aataaaagca tgcgaatcgt atcctaatcc
     5341 cgtacgcaac atttttaaaa ctttctgctt tcttcgtctt tcacactttt taaattttaa
     5401 aatttttaat tttttaaaaa aattcagaac tggcggcggt tgttttcact agagcagcct
     5461 ttatagaagg ttaactaatc tttacgcaac agacaagcat tattgcaaat tccggccaaa
     5521 gaaactacaa tgcccaaacc cggtcaaccc agccggataa ctggaataaa ctggacacaa
     5581 agcttcaagt tacaagaacc aacttacagg caactttaaa gaatcaattt tcaagggtat
     5641 cgccaatcag atatttgaaa gttaaaagtt aaaagttaaa aagacctgct tgattcttgt
     5701 tcctttattt ttgaaatttt taagcatgga gtttttcatg gatgtatatc ctttaaaatt
     5761 caaccccata tacaaggaaa agatatgggg cggccgcagt ttcgagcgac tgtttcacag
     5821 agaactgccg ccagatacaa atatcggtga aagctgggag ttagccgatt tgccagaagg
     5881 cgtaagcata gtatccaacg gagaatacgc cggcagaacg ctgagcgaca taaccgtttc
     5941 gctgggcgaa aaactgctgg gccaaacccc cgcaatgccg gatggccgtt ttcccctttt
     6001 gctgaaattc cttgacgcag aagacatact ttcacttcag gttcatcccg ataaaaccgc
     6061 cgtggcccaa atcggcggca aagccgcact caaaaccgaa tgctggtaca tcgtcgagag
     6121 tcgcgagggt tatatttatc gcggcattat tccgggcact acgccggcag aattcaagca
     6181 ggctatcgaa accgaccaag ccgaaaaata cgtcaagcga ta
//
LOCUS       DAONFI010000111         6216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14781_length_6216_cov_4.130336, whole genome shotgun sequence.
ACCESSION   DAONFI010000111 DAONFI010000000
VERSION     DAONFI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14781_length_6216_cov_4.130336"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLK08_04800"
     CDS             <1..254
                     /locus_tag="PLK08_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52854.1"
                     /translation="IATSYKSQGGPIFPVVQQKTWQYDGLDKRGLTPAKPTDLVWSML
                     KLICAISALKNTKSIAFRHYSLFFTSNGLFRFFYRRSGI"
     gene            316..2028
                     /locus_tag="PLK08_04805"
     CDS             316..2028
                     /locus_tag="PLK08_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPS52855.1"
                     /translation="MEFKHANKVFISLFVMAISFSTNAFAAREIRCGLPYIPPLMYRN
                     QEGKPAGLCIDLLTYLAKQHDWKLVFVDGTWDNCLLWLKEGKIDILPSAIRIPERDHD
                     YDFSSEPIVRIWNVLIVPADSKIKNVMQLENQKIAVLAGDVNVRNFVKLTDSYSVTWQ
                     PVPVESYAEVKAMVANHAVAGGLMNRFSAWTIDNPNIKHTDITSIPVATYFMTSKGKN
                     GDILKAIDRGMISLTNDPQSYYYLLLDKYLDENSKLHFEESFFEKYKGPIIWALGIVS
                     GFLVVIMIFLAALKAEVNRQSHLLMQSEDALKQAHEKELLIGSKIQKTILTNLPPADM
                     KGLEIAQMLIAAKHVGGDFTDYYMHNSHTVDVVIADVMGKGVPGALLGAAVKHALYKT
                     VHQTLPSPKNPPSVADIINQTNRNIVHELISLEQFVTLLYLRINTADMKMEMVDCGHS
                     LALWYQASTDKLQKLHSTNMPIGFVDREKFTQSTYDISAGDIIVLYTDGIPDTTNTAG
                     KIYGQSRIEQVFMEYRNESAEMILHAIQNDLQAFMGIQPQKDDFSCVVIKILPQNKNN
                     TQPA"
     gene            complement(2016..2780)
                     /gene="ccsA"
                     /locus_tag="PLK08_04810"
     CDS             complement(2016..2780)
                     /gene="ccsA"
                     /locus_tag="PLK08_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPS52856.1"
                     /translation="MNLGYFMATYMFICLAAVFAVVSLICLAAKSSRASSALWAASVV
                     LMVDVVCRAFIENGLPLRDMFDVFLILAALYIPAGDLCRKLTGQTTIFADAIVQLLLL
                     CPLAFAFSPEAKPIAPILRSNLFAPHVLCYMLAYVLLFKAAVISSKVLRGIAAAEAVD
                     KIVVLAMPFMTAGLLLGAVWGKRAWGDWWNWDPKELWSLACWCVYFAYLHFRIAMPAK
                     QKTAIVISLAGFACVILTLVWVNLSGSFVGLHNYAG"
     gene            complement(2777..4123)
                     /locus_tag="PLK08_04815"
     CDS             complement(2777..4123)
                     /locus_tag="PLK08_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52857.1"
                     /translation="MDGRMRFFRQKSLGSFSAAAILLLLSGVCIYGAFMGADRAAEIF
                     NSSFAGVCWCILGFMFLFVAAGNLIRRKLFIAAVHLGCAAIIIGGLLGSERVCGWRGL
                     APARAWTELREGQTAKLGDIVMKLRNIRVEYYPVAHSMLAWKIYLETCSDKGEVRLTP
                     VRNVDNLPVAMKGFVIPGRKIMCEKFDWILPDSFTLPQLIWPNNAGWEYEPLVKNRQI
                     DIVSNMFTHLSATIEKFYDNLRFDINSNEQIMAIDSDDGRVNRAVSLVDANGRKHYVF
                     SPGETVRLNVFGQDGRMLMYLPVGVNRGGRASPFAVILRLRRDGTVMTGVTAAVENDR
                     QVRFSLLPLYETEQQWISDGRAGLLFRLEQPEKQVFAECVFDDEKFVRIGINRPAGYG
                     GYDFTISDYDIDGMSVKIFARRNCGIFWVWSGFVLLGAGVTGWCVLKLLNRRRAAA"
     gene            complement(4205..4951)
                     /locus_tag="PLK08_04820"
     CDS             complement(4205..4951)
                     /locus_tag="PLK08_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPS52858.1"
                     /translation="MGCDRIKQLEAENRTLSARLEKQEQDLNQAEIERDQLREELAES
                     KKALDDKNAMLASLDQGKEELIARLRDQEALIEKLKNRKFEVGKLPTGLDMALKEFAA
                     ANPDIVTFDSQNGMVKLKSDLTFDPGSTTVNPKASEALNKLGRILDSPQAKSFCIYIA
                     GHTDDMPIAKEGTRRRHPDNWYLSVHRAVGVEKAMVKAGIAPSRMCVMGFGEYHPIAP
                     NKAGHRGNVLNRRVELWIVPQGTFLTEFAK"
     gene            complement(5103..5939)
                     /locus_tag="PLK08_04825"
     CDS             complement(5103..5939)
                     /locus_tag="PLK08_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52859.1"
                     /translation="MAKMYYSEEETCQKLGVNSGGLMEQFVNTAKLQMYQDGAKHVFK
                     AGDVDKLAESLGTAATAAGSADADAINLSELDDAAEISVGGKEDTVITSEGISIFDDE
                     DLQIDNAGTLSDTAPAPNVDDEVALGGVGSGSGLLDLTRESDDTSLGADLDLLDNLDV
                     EGMSGSGLGSGLGSGLVESGDVDTSMSTIASPAISYEPPTFVEEIDPTSGVFTGLLVG
                     ACLAGFMGLMAGLAAMTGDSVPGYLSAMQANMGMVLAIVVVFSVIAGGAGMIVGKAFQ
                     TK"
     gene            complement(5966..>6216)
                     /locus_tag="PLK08_04830"
     CDS             complement(5966..>6216)
                     /locus_tag="PLK08_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52860.1"
                     /translation="YIVAGQISSNTYGLYLVDYENQTICVYQYSPGDRKLRLMSARTY
                     RFDVNLDEFNTSDPTPREVRDLVGRQHRLAMPADNVEK"
BASE COUNT         1624 a         1688 c         1419 g         1485 t
ORIGIN      
        1 gaatcgcaac atcctataaa agtcaaggcg gtcccatatt tcctgtggtg caacagaaaa
       61 cctggcaata tgacggtctg gataaacgag gcttgacgcc ggcgaaaccg accgacctgg
      121 tctggagcat gttaaaattg atatgcgcca tttctgcttt gaaaaataca aaaagcatcg
      181 cttttcggca ttattccctg tttttcacaa gcaacggtct tttccggttt ttttatcgcc
      241 gcagcggtat ataatttatc tgtgaatgcg caatatcgta tagctgaaat tttgctgaat
      301 tttttttgaa taaccatgga attcaaacac gcaaataaag ttttcatatc actgttcgta
      361 atggcgataa gcttttcgac gaacgccttc gccgcgcggg agataaggtg cggccttccg
      421 tatattccgc cgctcatgta tcgtaatcag gaaggcaaac ccgcgggcct ctgtatagat
      481 cttctgacat atctggccaa acagcacgat tggaaactcg tttttgtgga cggcacgtgg
      541 gataactgtc ttctgtggct aaaggaaggg aaaatcgaca tcctgccaag cgcgatacgg
      601 attcctgaac gtgaccatga ttatgatttt tcgagcgaac ctatcgtcag gatatggaac
      661 gtcctgatag tgccggctga ttccaaaatc aaaaacgtca tgcagctgga aaatcagaaa
      721 attgcagtat tagccggcga tgtcaatgtg cgcaatttcg ttaagttgac cgattcatat
      781 tctgtcacgt ggcagccggt gccggtggaa agttatgctg aagtcaaggc tatggtcgcc
      841 aatcacgcag ttgccggcgg gcttatgaac cgcttttccg catggaccat cgacaatccc
      901 aacataaaac atacggacat cacgtccatt ccggtcgcta cgtatttcat gacgtcaaag
      961 ggaaaaaacg gcgatattct aaaggctatc gacaggggca tgatttcgtt gacaaacgat
     1021 ccgcaatctt attactacct gctgctcgac aaatatctcg atgagaactc caaactgcat
     1081 tttgaagaat cgtttttcga aaaatacaaa ggccccatta tttgggcgtt gggaatcgtc
     1141 agcggttttc tggtggttat aatgatattt ttggctgcac tgaaggctga agtaaatcga
     1201 caaagccatc ttctgatgca gtcggaagac gccctcaagc aggcacacga aaaggaactg
     1261 ctcataggct caaaaataca aaaaaccatt cttacgaatc tgccgccagc cgacatgaaa
     1321 ggccttgaaa tagcacagat gctcattgcg gccaaacatg tcggcggaga ttttaccgat
     1381 tattacatgc acaacagcca tacggttgac gtggtcatcg ccgacgtcat gggaaaaggc
     1441 gtgccaggcg cactgctcgg cgcagccgtc aaacacgcac tctataaaac cgtccatcaa
     1501 actctaccgt cgccaaaaaa tccaccgtca gtagccgata ttataaatca gacaaaccgt
     1561 aatatagtcc acgaactcat ttcacttgaa cagttcgtaa cgctgcttta cctgcgaata
     1621 aacacggctg acatgaaaat ggagatggtc gactgcggcc attcgctggc actgtggtat
     1681 caggcgtcta ccgacaaact gcaaaaactt cacagcacaa atatgcccat aggttttgtc
     1741 gacagggaaa aattcacaca atctacttac gacatctctg ccggcgatat tatagtgctg
     1801 tatacggacg gcattcccga cacaaccaac actgccggca aaatttacgg ccaatcgcgt
     1861 atcgagcagg tttttatgga atatcgcaac gaatcggcgg agatgatact tcatgccata
     1921 cagaacgatt tgcaggcatt catgggcatt cagccgcaaa aggacgattt ctcctgcgtc
     1981 gtcatcaaaa ttcttccgca aaataagaat aacactcagc cggcgtaatt gtgaaggccg
     2041 acaaagctgc cggaaagatt cacccatacc agcgtaagaa tgacgcacgc aaatcccgcg
     2101 agggatatga ctatagccgt tttctgttta gccggcattg caatcctgaa atgcaggtat
     2161 gcaaaataaa cgcaccagca ggcaagcgac cagagttcct tcgggtccca gttccaccag
     2221 tcgccccatg cgcgtttgcc ccaaaccgcg cccagcagca ggccagccgt catgaacggc
     2281 attgcaagta cgacgatttt atcaaccgct tcggcggcag ctatgccgcg caacactttt
     2341 gacgaaatga ccgcagcctt gaaaagcagc acataggcaa gcatataaca caatacgtgc
     2401 ggagcgaaaa gattgcttcg taatatcggc gctatcggtt tcgcctcagg gctgaatgca
     2461 aaggcaagcg gacagagtaa aagcagttgt acgattgcgt cggcaaaaat ggttgtctgg
     2521 ccggtgagtt ttcggcacaa atcaccggct ggaatatata gcgcagcaag aatcagaaat
     2581 acgtcaaaca tgtctcgcag cggcaggccg ttctcgatga acgcgcggca gaccacgtcg
     2641 accataagca cgacgcttgc cgcccataat gccgacgaag cgcgggacga ttttgccgcc
     2701 agacatatca acgacactac agcaaaaacg gcagccagac agataaacat gtatgtggcc
     2761 atgaaatacc ccagattcat gccgcagccc tcctgcgatt aagcaatttt agcacgcacc
     2821 agccggtcac tccagccccg agcaatacga atccgctcca cacccagaag attccgcaat
     2881 tacggcgggc gaaaattttc acactcatac catcgatgtc ataatcggaa atcgtgaaat
     2941 catacccgcc atagccggct ggcctgttta tgccgattcg cacaaatttt tcgtcatcaa
     3001 aaacgcattc ggcaaaaacc tgtttctccg gctgttcaag gcggaaaagc aaccctgccc
     3061 tgccgtcgga aatccattgc tgttcagttt catacaacgg caacagcgaa aatcttacct
     3121 gcctgtcatt ttcgacggca gccgtcacgc cggtcatgac cgtgccgtcc ctgcgaagcc
     3181 gtaaaatcac agcaaacgga cttgcccttc caccacgatt cacaccaacc ggcagataca
     3241 tcaacatcct gccatcctgc ccgaatacgt tcagccgtac cgtttctcca ggcgaaaaaa
     3301 cataatgttt ccggccattc gcatcaacca gactcactgc gcgattcact ctgccgtcgt
     3361 cggaatcaat cgccataatc tgttcgttcg aatttatatc gaatctgaga ttatcataaa
     3421 atttttcaat cgtcgccgac agatgcgtga acatgtttga aacaatatct atttgccgat
     3481 tctttaccag cggttcgtat tcccagccgg cattgttcgg ccagattaac tgcggaagcg
     3541 taaaactgtc cggaagaatc cagtcgaatt tttcgcacat gatttttcgg ccaggtatta
     3601 caaaaccctt catcgcaaca ggcagattat caacattccg aaccggagtc agccggactt
     3661 ctcctttatc agaacacgtt tcaaggtata ttttccaggc aagcatggag tgcgcgacag
     3721 gataatactc cacgcgaatg ttgcgcaatt tcatgacgat atcgcccagt ttagccgtct
     3781 gtccctcgcg aagttccgtc catgcccgcg ccggcgcaag gcctcgccag ccgcataccc
     3841 gttcactgcc gagaagcccc ccgataatga ttgctgcaca gcccaggtgt accgccgcaa
     3901 tgaataattt tcgacgaatc agattgccgg ccgcgacaaa cagaaacata aatcccaaga
     3961 tacaccagca cacgccggcg aaagaagaat tgaaaatctc cgccgccctg tccgccccca
     4021 tgaatgcacc atatatgcag acacccgaca gcagcagcaa tattgcggca gctgaaaacg
     4081 accccaaact tttttggcgg aaaaatctca ttcgtccatc catgacaaag cccagccatt
     4141 gctgactggg ctttgagtgt tattggccat ttgatttttt aattttaagc gattttgagg
     4201 aaaatcactt tgcgaattcc gtcaaaaacg ttccctgcgg aactatccag agttcgacgc
     4261 gtctgttgag gacgttgccg cggtggccag ccttattagg tgcaatcgga tgatattcgc
     4321 caaaacccat tacacacatg cgtgaaggtg caatgccggc tttgaccata gccttttcaa
     4381 cgccgaccgc gcggtgcacg gacagatacc agttgtcagg atgacgacga cgagtaccct
     4441 ctttggcgat gggcatatca tcggtatggc cggcgatata gatgcagaaa ctcttggcct
     4501 gcggagaatc aagaatccgg ccgagtttgt tcagagcctc tgaagccttc gggttcaccg
     4561 tagtgctgcc cggatcaaac gtcagatcac tcttgagctt gaccatgccg ttttgggaat
     4621 cgaacgtcac aatatcagga ttggccgccg caaattcctt caacgccata tcaaggccag
     4681 tcggcagttt gcctacttca aacttgcggt ttttgagttt ttcgataagc gcttcctggt
     4741 ctctcagcct ggcaataagt tcttccttgc cctggtcgag cgacgccagc atggcgtttt
     4801 tatcatccag agcctttttg gactcggcga gttcttctcg caattggtcg cgttctattt
     4861 ccgcctgatt caggtcctgt tcctgttttt caagacgggc gctaagcgta cggttttcag
     4921 cttccagctg ttttatccta tcgcagccca taaacatgcc ggcagcgaca acacatgtcc
     4981 caataatcaa taaatttttc gtggcattca taataaagcc cctttcgttt ttctgatttt
     5041 ttaacaattt ttttaaaaaa taccggctat ctatcaagcc gatatgatag tttgaaaaca
     5101 ttttatttgg tctggaaggc tttgccgact atcatgcctg cgccgccggc tatcacggag
     5161 aacactacga ctattgctag taccatgccc atgttcgcct gcattgccga gagatatccc
     5221 ggcacgctat cgccggtcat cgccgccagc ccagccataa ggcccatgaa accagccaga
     5281 cacgcaccta ccagcaagcc ggtaaaaacg ccgctggtcg ggtctatttc ttccacaaat
     5341 gtcggcggtt cgtagctgat ggccggcgaa gcaatggtgc tcatggacgt atcaacgtcg
     5401 ccggactcca ccaggccact gcccaaaccg ctgcccagcc cgctgccgct catgccttcc
     5461 acatccagat tgtcaagcag gtcaaggtcc gcaccgagcg atgtgtcgtc gctctcgcgc
     5521 gtgagatcaa gcaggccact gccgcttcct acgccgccga gcgcgacttc atcgtcaaca
     5581 ttcggagccg gcgccgtatc cgacaatgtg ccggcgttgt ctatctgaag gtcttcatca
     5641 tcgaatatgc tgatgccttc ggacgtgata acggtatctt ctttgccgcc gacggagatt
     5701 tccgcggcat catccagttc ggaaagattt atggcgtcag cgtcggcact accggcagca
     5761 gtagcggcag tacccagtga ttcggcgagc ttgtcgacat cgccggcttt gaaaacgtgt
     5821 ttggctccgt cctgatacat ctgcagctta gccgtattga cgaactgttc catcagtccg
     5881 ccgctgttta cgcccagctt ctgacaggtt tcctcttcgg agtaatacat cttggccatt
     5941 tctgactcct ggggttgcgt ccggttcact tctcgacatt gtcagccggc attgcaagcc
     6001 tgtgctgcct gccgaccaga tctcgcactt cacgcggtgt agggtcggac gtgttgaact
     6061 catcgagatt cacatcgaag cgatacgtcc tggccgacat cagccgcaat ttccgatcac
     6121 ccggactata ctgatataca caaatcgtct gattttcata atcgacaagg tacaatccgt
     6181 aagtgttcga ggatatctgg ccggcaacta tgtata
//
LOCUS       DAONFI010000112         6208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14807_length_6208_cov_4.339672, whole genome shotgun sequence.
ACCESSION   DAONFI010000112 DAONFI010000000
VERSION     DAONFI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14807_length_6208_cov_4.339672"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLK08_04835"
     CDS             complement(<1..265)
                     /locus_tag="PLK08_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HPS52861.1"
                     /translation="MLYFVRKTADRLFTGISLLSILLMIAALVIFLYPIFQKGSKAVI
                     FNGTVEFRKMQFELFHRGDGEKLDAQTRAANHYKAVAFDMLDNF"
     gene            complement(265..1233)
                     /gene="pstC"
                     /locus_tag="PLK08_04840"
     CDS             complement(265..1233)
                     /gene="pstC"
                     /locus_tag="PLK08_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPS52862.1"
                     /translation="MNENMLKIDRPVNIGNFDRPLQLTAGQDMAGFLYKRIGRFLLFT
                     LTCASVLAVFLIFIFIITEASSFFSFRRLGEMLSSTRWAPESLRPQFGALAIICGSLY
                     VTAGALILAVPAGVMSAMFLSDIAPFSFRQLVKPIIEILAAIPSVAYGFFAVMILAPW
                     LQNTLHLPTGTNAMNAAMILAIMAIPTIVSVSEDSLSAIGREIREGSYALGATRAETL
                     LKVVMPAAGRGITTAILLGMMRAIGETMVVWMASGNATQIPSPWWDISQSVRTLTATI
                     AGDMGETAKGSDHYHALFALGAMLLIFTMIINFVGETLLAAGRGKH"
     gene            complement(1324..2175)
                     /locus_tag="PLK08_04845"
     CDS             complement(1324..2175)
                     /locus_tag="PLK08_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPS52863.1"
                     /translation="MSKTTMNLKLTAAGVLLISTVLTFAVAANDSVKELTVEGSTTVG
                     PIADAFAETLMRKNPGLQISVKKTGSGDGAAALIDGRCDVATMSRFMKPEEFKKAVDK
                     GIYPVANTIAMDGVCIILHPSNPVKALTLEQIKGIYEGKITNWNELGGADTPIVAISR
                     DTSSGTYEVFHEKAMHKQDMGSKVEYVNSNPQAHARVKTTPGAIAYVGLGFVDNDVKA
                     VTVNGIAPTRDTIATGEYPISRALYMFTNGETKPGTPAHIFVKFYLSEAGQEIIEAKG
                     FVPVTKY"
     gene            2409..3098
                     /locus_tag="PLK08_04850"
     CDS             2409..3098
                     /locus_tag="PLK08_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS52864.1"
                     /translation="MSKKRIILVEDEKDMADLVAMQLRREHYEVMTAHDGNDGMMLIR
                     REIPDLVLLDLMLPGMSGMEIASQMKGDQNLRDIPIIMLTAKGEDADVVVGLQLGADD
                     YITKPFSMKILIARISAVLRRSETRDESPIIETLGLIKLDRGKHEVSVADKPVILTLT
                     EFRILESLIAARGRVLNRNQLIDKALGFDAVVTDRTIDVHINSLRKKLGKARDYVHTV
                     RGIGYRFTGEE"
     gene            3102..4907
                     /locus_tag="PLK08_04855"
     CDS             3102..4907
                     /locus_tag="PLK08_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPS52865.1"
                     /translation="MRFFYAKSFFIRLFFSTLILAIVMVIAVNLIIYEQLNLNYQERS
                     KQNQEQYTRLLLKYFTNAMAEHPAEIDRECKNLSSETRYRVTVIAADGSVLGDSLVSP
                     ESMENHKTPNRPEVWNAFNKEMHSDIRHSSTMEVDFRYCAVPVIVDGRVAGIVRTAMP
                     VVTIAQGEGVIRQILLWASVATLIMSAGLGLLISWLWNRPLKEITLAARRLAGGELDQ
                     KIHITAGTKELSQLAGSLNEMREKLAGKISQITSQRENLHAVVENLAEGVIALDYDDR
                     IIQLNAAACRLIGIPVAACLDRILQNAVRIPDILDAVSRSRTHNESVRTQIRLDNGNI
                     LDVQVYSVHDGGDNDIAVLIVLRDMTEAEKMTAIKSQFVANASHELRTPLATIRAAVD
                     SLKLEGAGDASMFERLLDILDRHTRRLEDMTNDLLDLHMVESSGRLSRRDSVALGRLR
                     DWVLNNFASKADEKKISLAVKCSSPEFVFSTDLTLVHLILQNLLTNSIKFTPADGAIT
                     CTLIPDEDFLLMRVADTGCGIPPEFHDRVFERFFQVSASRSRDNGNISGGHQNGGTGL
                     GLSIVKHAAMKLGATISLESEPGRGTSITIQLPLR"
     gene            complement(4942..5973)
                     /locus_tag="PLK08_04860"
     CDS             complement(4942..5973)
                     /locus_tag="PLK08_04860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPS52866.1"
                     /translation="MSDNSKARPPRTKRSKIKVKYIFLCLLILVIAGTAVTAFIPVTH
                     WVFGTGYIITSRDAEIRPPLEATIEKWLVNDGSLVKKNQPIIQLASTVETAALESAKN
                     ELETAKAKLKSLQASLELQTASLKQQKYRAQRVLEQAEKDLQSMKTAPANVFSPKEFD
                     DAKLRRDNANSMLRELSLPQDELLKKQIDIAREEVETAVKKVCVRQAEVDMRQICASI
                     DGTVYFNRFDEGEVVKPSDVLGQIFDKTEWVVRLKIAESDLRYVRLEQRVEIEVSGFW
                     QIRYGYLMGKVCKITPVVSPQMTGSGVFTLKAMIDDPHDKRLNPGVSVTAWVNVGEIS
                     LLRKILGLY"
BASE COUNT         1476 a         1518 c         1633 g         1581 t
ORIGIN      
        1 aaaaattgtc gagcatgtca aacgcgacag ctttgtaatg attggcggct cgcgtttggg
       61 cgtcgagttt ttctccgtcg ccgcgatgga agagttcaaa ctgcattttg cggaattcaa
      121 cagtgccgtt gaatattacg gctttcgagc ctttttgaaa tatcggatac aggaaaatga
      181 cgagcgcggc tatcatcagc aggatgctca gcagcgatat gccagtaaaa agtctgtctg
      241 cggtttttct gacgaaatat agcattagtg ttttccacgg ccggcggcga gcagcgtttc
      301 gccgacgaag tttattatca tggtgaaaat cagcaacatc gctcccaatg cgaacagggc
      361 atgataatgg tcagagcctt ttgcggtttc acccatatcg ccggcgatag tagcggtcag
      421 cgttcgcacc gattgcgaaa tatcccacca tggacttgga atctgcgtgg cgttgccgct
      481 agccatccag acgaccatcg tttcgccgat agccctcatc atgccgagca gaattgccgt
      541 ggttatacca cggccggctg ccggcattac aactttcagg agcgtttcag cccgcgtggc
      601 gccgagagca tacgacccct cgcgaatctc cctgcctatc gccgaaagcg aatcttccga
      661 cacgctgacg atagttggaa tggccattat cgccagtatc attgccgcgt tcatggcgtt
      721 tgtgccggtc ggcaggtgga gcgtgttctg cagccagggg gctagaatca tcaccgcgaa
      781 aaaaccgtag gcgaccgatg gaatagctgc cagaatttca attataggtt ttaccagctg
      841 tcgaaagctg aacggtgcta tatcgctcaa aaacattgcc gacatcacgc cggccggcac
      901 ggccagaata agcgcgccgg ctgttacgta aagtgagccg catattatcg ccagtgctcc
      961 gaattgcgga cgcagactct ccggtgccca cctggtcgac gaaagcatct cgcccagacg
     1021 cctgaatgaa aaaaacgacg acgcctcggt tatgatgaat atgaatatca ggaatacggc
     1081 gagtacgctc gcacaggtaa gcgtaaacag cagaaatctg ccgattcgtt tataaagaaa
     1141 accggccata tcctggccag cggtcagttg cagcggccta tcgaaattgc cgatgtttac
     1201 gggtcggtcg atttttaaca tattttcgtt cataaaatta tgtttgcgtc accacgggaa
     1261 accggcaggg ggtttcccgt ggtgttcgca gagcgacgga ggtcgctatc atatgtgtgt
     1321 atctcaatac tttgttaccg gaacgaatcc tttggcttcg atgatttcct ggccggcttc
     1381 gctgaggtag aatttcacga atatgtgtgc cggcgtgcca ggtttcgttt cgccgttggt
     1441 gaacatgtac agtgcgcggc tgattgggta ttctccggtc gcgatggtgt cccgcgtcgg
     1501 agctatgccg ttgacggtaa ccgccttgac gtcattgtcg acaaaaccga ggccgacgta
     1561 agcaattgcg cctggcgtcg tctttaccct tgcatgtgcc tgcggattgg aatttacgta
     1621 ttcgactttt gaacccatgt cctgtttgtg catggctttt tcgtggaata cttcgtaagt
     1681 gccggaagaa gtatcgcggc tgatggcaac tatcggcgtg tcagcgccgc cgagttcatt
     1741 ccagttggta atcttgcctt cgtaaatgcc cttaatctgt tcgagggtca gcgcttttac
     1801 cggattggaa ggatgcagaa ttatacaaac gccgtccatt gcgatggtgt ttgcaactgg
     1861 atatatgcct ttgtcgacgg cttttttgaa ttcttcaggt ttcatgaaac ggctcatggt
     1921 ggctacgtcg caacggccgt ctataagtgc ggctgcaccg tcgccgctgc cggtcttctt
     1981 gaccgaaatc tgcaatccag gatttttgcg catcagcgtt tcggcaaacg catcggcaat
     2041 ggggcctacc gtggttgagc cttcgacggt caattccttc actgaatcgt tcgccgcgac
     2101 ggcaaaagtc agcaccgtgc tgataagcaa tactccggca gccgtcagct tgaggttcat
     2161 agttgtcttt gacatttctt ttctccttca aaaaatctcg tcaaacatat gcccgggaac
     2221 ttcccgagcc acaaccgaca tgatgccgca acaacaaaaa gaaaccatta ggacaacatt
     2281 aagattgcat tattaatgat gccgattttc catcaatatt gttaagattg cgttaagagg
     2341 ttttaaagat atcgataaac tgcttaaagc ggcttgtttg aggttaaaat taggttacaa
     2401 ttcaggccat gagtaaaaaa aggataatat tggtcgagga cgaaaaagac atggctgacc
     2461 tcgtggcgat gcagctgcgg cgggaacatt atgaggttat gacggctcat gacggaaacg
     2521 acggcatgat gctgattcgc cgtgaaattc cggatttggt attgctcgac ctgatgctgc
     2581 cgggcatgag cggcatggaa atagcgtcgc agatgaaggg cgaccagaat ttgcgggata
     2641 tcccgataat catgcttacc gccaaaggcg aagatgcaga cgttgtcgtt ggattgcagc
     2701 ttggtgctga cgattacata accaaacctt tcagcatgaa aattcttatt gcgagaatat
     2761 cggcggtgct gcgtcgcagc gagacgcggg atgagtcgcc gataatagaa acgctgggac
     2821 ttataaagct cgacaggggc aaacatgagg tcagcgtggc tgataagcct gttattctga
     2881 cgctgacgga atttcgcatt cttgaatcgc tcattgcggc gagaggccgg gtgctcaatc
     2941 gcaatcaact catcgacaag gcgctgggat ttgacgcggt tgttacggac agaaccatcg
     3001 acgtacacat aaattcgctg cgaaaaaaac tcggcaaagc ccgtgattat gttcataccg
     3061 ttcgcggcat agggtatcgt ttcaccggag aagaataatc catgcgattt ttttacgcga
     3121 aaagtttttt catacgtctg tttttcagca ctctgatttt ggcgatagtc atggttatcg
     3181 cggttaatct gataatttac gaacagctca acctgaatta tcaggaacgc tcgaagcaga
     3241 atcaggaaca atatacaagg ctgctgctca aatattttac gaacgccatg gcggaacatc
     3301 ctgccgaaat tgaccgcgaa tgcaaaaatc tgtcaagcga gacacgttac agggttaccg
     3361 tcatagcggc tgacggaagc gtattgggtg attctcttgt ttcgccggaa agcatggaaa
     3421 atcataaaac gccgaatagg ccggaagtct ggaatgcatt taataaggaa atgcattccg
     3481 atatacggca cagttccacg atggaagtag atttccgtta ttgtgccgtg ccggttattg
     3541 tagacggccg tgtcgccggc atcgtcagga cagccatgcc ggtcgtaacc atcgcacagg
     3601 gtgagggggt catacggcag atactgcttt gggcatccgt tgcgacgctg ataatgtcgg
     3661 caggcctcgg attgctcata agttggctgt ggaacaggcc gctgaaggaa ataacgctgg
     3721 cggccagacg tctggctggc ggcgaacttg accagaaaat acatataaca gccggcacaa
     3781 aagagctgtc gcagttggct ggctcgctta atgaaatgcg ggaaaagctc gccggcaaaa
     3841 tcagccagat aacctcgcag cgcgaaaatc ttcacgccgt cgttgaaaat ctcgccgaag
     3901 gcgtcatagc cctcgattac gatgacagga taattcagct caacgccgcg gcatgccggc
     3961 tgataggtat tcctgtggcg gcgtgcctgg acagaatttt gcagaatgcc gtccggattc
     4021 cagacatcct cgatgcagtt tcacgttccc gcacgcataa cgagagcgtc aggacccaga
     4081 tacgcctgga taacggcaat attctggatg tgcaggtata ttccgtgcat gacggcggcg
     4141 ataacgacat cgccgtattg atagtgttgc gagatatgac cgaggccgag aaaatgacgg
     4201 cgataaaatc gcagtttgtc gccaacgcca gccatgagct tcgtacgccg ctggctacca
     4261 ttcgcgctgc ggtcgattcg ttgaaactgg aaggtgcggg tgacgcttcg atgttcgagc
     4321 ggctgcttga cattctggac agacatacgc ggcggctgga agatatgacg aacgatttgc
     4381 tcgatttgca catggttgaa agctccggcc gactgtccag acgggattcc gtcgcacttg
     4441 gccgcctgcg ggattgggtg ttgaacaatt ttgcgtccaa agccgacgag aaaaaaatat
     4501 cgcttgcggt aaaatgttca agccccgaat tcgtgttttc cacggatttg acgcttgtgc
     4561 atcttatatt gcagaatctt ttgaccaact cgataaaatt tacgcctgcc gacggggcaa
     4621 tcacctgcac gctcattccc gacgaggatt ttctgctaat gcgcgttgcg gacacgggct
     4681 gtgggattcc tcctgaattt cacgaccgcg tattcgagcg gtttttccag gtaagcgcgt
     4741 cgagaagccg cgataacggc aatatcagcg gcggccatca gaatggcgga acaggcctcg
     4801 gccttagcat cgtcaaacac gcagccatga aactcggcgc aaccatcagc cttgaaagtg
     4861 aacctggcag ggggacgtct attacaattc aactcccact gcggtaaaaa acgattatat
     4921 tcgcagtggc ggcggctgac atcaataaag gccgagtatt ttccgcaata gcgatatttc
     4981 ccctacgttc acccacgcgg taacgctgac gcctggattc agccgtttgt catgtgggtc
     5041 gtctatcatg gcttttaacg tgaagacgcc gctacccgtc atttgtggcg aaacaacggg
     5101 cgttatcttg cagaccttgc ccatgagata cccgtaacgg atttgccaga agccggacac
     5161 ttcaatttca actctctgtt ccaatctgac gtacctcagg tcgctttccg cgattttgag
     5221 gcgaacgacc cattcggtct tgtcgaatat ctggccgagg acgtcggaag gcttgacgac
     5281 ttcgccttcg tcgaaacggt taaaataaac tgtgccgtcg atactggcgc atatctgtcg
     5341 catgtcgact tcagcctggc gtacgcaaac ttttttgacg gctgtttcga cttcttccct
     5401 ggcgatgtct atctgttttt tcagcagttc atcctgcggc agagaaagtt cccgcagcat
     5461 gctgttggcg ttgtcgcgac gtaattttgc atcgtcaaat tcctttgggg aaaaaacatt
     5521 ggcaggtgcg gtcttcatgc tctggaggtc tttttccgcc tgttcaagga cgcgctgtgc
     5581 gcggtatttc tgttgtttca acgaggcagt ctgcaattcc agcgaggcct ggagcgattt
     5641 cagtttcgcc ttcgcggttt cgagttcgtt cttggcgctt tcgagagctg cggtttctac
     5701 ggtactggcg agttgtatta tcggctgatt ttttttgaca agcgagccgt cgtttaccag
     5761 ccatttttca atggtggctt caagcggcgg ccgaatctcg gcatcgcggc tggttatgat
     5821 gtaacccgtg ccgaagaccc agtgcgtaac cggtatgaat gccgtcactg ccgtgcctgc
     5881 aatcaccagt ataagcagac acagaaaaat gtatttcact ttgatttttg agcgttttgt
     5941 ccgcggcgga cgggctttgc tgttatccga cattttttta cttcactttc ggcagtttta
     6001 aaaattcaaa aagggttttt attacatatt tacaaccgtc agtctttaga gcattttaaa
     6061 cttgatatgc gtcatttctg ctggctgcaa catcgctctg ctacgtcagg ctgcatcgga
     6121 atacgggcag tattcctgct tcgccttcct tgcatagctc gtttcgccaa cgcataaagt
     6181 gtcgtatctc aagtttaaca tgctctag
//
LOCUS       DAONFI010000113         6201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14833_length_6201_cov_4.273674, whole genome shotgun sequence.
ACCESSION   DAONFI010000113 DAONFI010000000
VERSION     DAONFI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14833_length_6201_cov_4.273674"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLK08_04865"
     CDS             <1..595
                     /locus_tag="PLK08_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52867.1"
                     /translation="LLCSLLSMPQVRDAKAVATLAEQLQLYSGTDLPITRHYVTTALN
                     DATSAGAIVKRIYWIKTLNFYRLSQNPDGGWGFSENRPTNYIPTLIALNDMIISLKQL
                     GKDDATIREHPNVKKGMEWIGKNFENDVTADIIQYYLLLTQLASLRGTAIIDNRDSMQ
                     FVKDEIFRIQDKNGSWQVEGSSPYVSTAMALLCLTAK"
     gene            complement(603..2330)
                     /locus_tag="PLK08_04870"
     CDS             complement(603..2330)
                     /locus_tag="PLK08_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPS52868.1"
                     /translation="MKVLIHDALRKEWLEFSEPAEIFHAATPVGVLPALSRAAVVSRE
                     KNLPLAGWLSYESAGGINPDLPYRESAFPLVNFAAFQRCMPVTLTAVCRPAAVTWRSE
                     LARKDYFEKFSQIRRYIADGETYQVNYTFRMRCDSFDSGMAEKYWLAMIAAQRSRYGS
                     FIETDDWAICSASPELFFSYDGGVVTCRPMKGTAPRGLWPAADERAAAELADSEKNRA
                     ENIMIVDMTRNDLGRVAQPGSVEVACLCGLERYPTLWQMVSEIRCRCECGWVDVLKAL
                     FPCASITGAPKRRTMQIIDGLETSPRNIYTGTIGFVLPDGRAQFNVAIRTALADKKNA
                     TAEYGVGGGIVWDSADCGEYDEAMLKAKVLLRPSPRPELLETLLWEDGGYFLFERHMQ
                     RLAASAKYFDYTFKCDSVKKQLTGYAVKNFIAGLRYRVRLVMNEYGKISLEHFRIDGP
                     ADSPVRIALADKPINRNDVLLYHKTTSREVYERAKAARDDVDDVLLFNDQNLLTESTI
                     SNIVLKIKGRLLTPDISAGLLGGVFRQELIDNGKIQSADLPVELLKNCDEIWLINSVR
                     KWRKAVMVG"
     gene            2538..3503
                     /locus_tag="PLK08_04875"
     CDS             2538..3503
                     /locus_tag="PLK08_04875"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52869.1"
                     /translation="MNQEAFTNQLLRHLQQDFPLCEKPFAQLANELGVDENSLIEQVR
                     CLKSCGIIRRISGTFNLRKFGYHMALVAFDVAPDKMHIAANVLVEQPAVSHCYVRQDN
                     DFAIWFTLALPERLDEQVASLANICKARRVLNLPATQHFKLSTMFDMETGCVLESPAE
                     AEKTQPLSLTPELKKIIHALQTSINISHKPFDGPAKTVGVSTDELFRGAEILRKAGLL
                     RRYSAVIDHAAAGIVANALVTWQTNPVEEEHAGILASLNPHVSHCYARKTCDGWPYNL
                     YTMIHARTQKKLDEVICELAATLGNPPRRILPTMHEIKKAPVLIS"
     gene            complement(3580..4860)
                     /locus_tag="PLK08_04880"
     CDS             complement(3580..4860)
                     /locus_tag="PLK08_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS52870.1"
                     /translation="MKFLRIWLGLLFFVSPVFAGEFSIIHQFDGVTSTMPYGSVILSN
                     GVLYGTTVGVSSSSGGGTVYKLNTDGSDFEVLHSFTSSTDGIEVYNGLTLVDGNIYGI
                     ARNGGSAGCGVLFKIDSSSAFSVIHNFTGSGEGSYPYTAPTYYNGSLYGLTWGGWASG
                     TSNIYQYNLSTGTYTSLYNMTSPGVQPFGTVTVINGYLYGMNSDNRDTANHGNIFKYN
                     LDSGSYEIVHTFAGGTSGGYPYDSLVWDGGTYVYGTTMGYAVWSEDNEESTPKRSEEL
                     ADEGVVFRLNIETNEYTVLHDFSAQTGDGAKPNSSMLIAPDGWLYGIAHGNEIWGGTE
                     YGTLYRMRLDGSDFEVLHVFDSMEDGNVPMRSIIWDDGIIYGTTAYGGYGTGDGYGTV
                     WMFNTLVPEPATLLILLVAAAISKRKRRQPPMRI"
     gene            complement(4956..5040)
                     /locus_tag="PLK08_04885"
     tRNA            complement(4956..5040)
                     /locus_tag="PLK08_04885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5004..5006),aa:Leu,seq:gag)
     gene            5197..5907
                     /locus_tag="PLK08_04890"
     CDS             5197..5907
                     /locus_tag="PLK08_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52871.1"
                     /translation="MLKKTILGDLHEKSARMGESGGWAVPLEFHGQGDAESILSGGVG
                     IFDLSHYSRLRLRGYSVPDMFDAVGVAGLASMDDNTVREINIADTRCTLVRLEDFWVV
                     VAPPDANELLLEKFRSADGVKADSQTMKVAMFALAGTTARQTLAGVMPVKLPSLANGQ
                     AISGSLMLANYIAYRNDVAGVPVINVMLPAIFASKAWNFITMNAGRNCAMPLGIDVWN
                     RLAFKNFFKMTSVCWRAF"
     gene            5973..>6201
                     /locus_tag="PLK08_04895"
     CDS             5973..>6201
                     /locus_tag="PLK08_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPS52872.1"
                     /translation="MEQAQMGQFADEFSNDTWRIFRIISEFADGYEEMSKLGPAVSVF
                     GSARISQNDPDYKKARQFTAMVAKRGFSIITG"
BASE COUNT         1579 a         1689 c         1507 g         1426 t
ORIGIN      
        1 cctgctatgc tcgctgctct caatgccgca ggtgcgcgac gcaaaggctg tcgcgacgct
       61 ggcggagcag ttgcagctct attccggcac ggatttgccg ataacgcggc attacgtcac
      121 tacagccctc aacgacgcca cctcagccgg cgcaatcgta aaacggatat attggataaa
      181 aacacttaat ttctaccgtc tcagccagaa tccagacggc ggctggggat tttctgaaaa
      241 ccggccgacg aattatatcc caacgcttat cgccttaaac gacatgataa tatcgctcaa
      301 acagctcggc aaggacgatg cgacaatccg cgaacacccg aacgtaaaaa aaggcatgga
      361 gtggattgga aaaaatttcg aaaacgacgt tacggccgac ataattcaat attatcttct
      421 gctgacgcaa ctggcgtcgt tgcgaggaac ggctataatc gacaacaggg actcgatgca
      481 atttgtgaaa gatgagattt tcaggataca ggacaaaaac ggcagttggc aggtcgaggg
      541 cagctcgcca tatgtctcaa ccgcgatggc cctgctgtgt cttacggcaa aataaccgga
      601 agctatccaa ccatcactgc ctttcgccat tttcggactg aatttatcag ccatatttca
      661 tcgcagtttt ttaacaattc cacaggcagg tcggcagatt ggattttgcc gttatcgata
      721 agttcctgcc ggaacacccc gccaagcagg ccggcggaaa tatcaggcgt aagcagccgc
      781 cccttaattt tcagaacaat attcgatatc gtggattccg taagcaggtt ttggtcgtta
      841 aacaacaata cgtcgtcaac gtcatcgcgg gcggctttcg cacgttcgta aacttcgcgt
      901 gaagtcgtct tgtgatacag caacacatcg ttcctgttta ttggcttgtc ggcaagtgcg
      961 atacgcaccg gagaatctgc cggcccgtca attctaaaat gttccagcga gattttccca
     1021 tattcattca tcaccagccg cacgcgatat cgcaagccgg cgataaaatt ttttacggca
     1081 tatccggtta attgtttttt tacgctatcg catttaaagg tgtaatcaaa atatttcgca
     1141 ctcgccgcca gccgttgcat atgtcgctcg aacagaaaat atccgccgtc ttcccacaac
     1201 aacgtttcaa gcagttccgg ccgcggcgat ggtctgagca gcactttcgc cttgagcatc
     1261 gcttcgtcat attcgccaca atccgccgaa tcccacacga tacctccgcc tacgccgtac
     1321 tcggcggtcg cgtttttttt gtctgccagc gcggtgcgga tggccacatt gaactgggcg
     1381 cggccgtccg gcagcacaaa accgattgtg cccgtataaa tatttctggg cgacgtttca
     1441 agcccgtcga taatctgcat ggttcgccgc ttgggcgcgc cggttatcga tgcacacgga
     1501 aagagcgctt tcaatacatc gacccagccg cactcgcaac ggcatctgat ttcgctgacc
     1561 atctgccata acgtcggata ccgttccagg ccgcacaagc aggcgacttc gacgctgcct
     1621 ggctgggcga cgcggcccag gtcgttgcgc gtcatgtcga caatcatgat gttttcggcg
     1681 cgattttttt cgctgtctgc aagttcggcg gcagcgcgtt cgtccgccgc cggccaaagg
     1741 cctcgcggcg cagtgccttt cattggcctg caagtcacca ctccgccgtc atacgagaaa
     1801 aaaagttcgg gcgaggctga acatatcgcc cagtcatccg tttcgataaa tgagccgtac
     1861 cggctgcgct gggctgcaat catagccagc caatactttt ccgccatgcc ggaatcaaac
     1921 gaatcgcatc gcatcctgaa cgtgtagttc acctgataag tttcgccgtc agcaatatat
     1981 cgcctgattt gcgaaaattt ctcaaaataa tcctttcgcg ccaactcgct ccgccacgta
     2041 accgcagccg gccgacacac cgccgttaac gtgacaggca tacatctctg aaacgccgca
     2101 aaattaacca acggaaaagc tgactccctg tacggcaaat cgggattaat tccgccggct
     2161 gattcgtatg acagccagcc ggcaagcggc agattttttt cacgtgaaac gacggctgcc
     2221 ctgctcagcg ccggcaagac accgactggc gtggcggcat gaaaaatttc cgccggctcg
     2281 ctgaattcca gccattcttt ccgcagtgcg tcatgtatca gaactttcat cgcacaaaga
     2341 atacaccatc caccgccagg tctgaaggaa aatagttgtt tcaggcaagg cgacaatata
     2401 aaatcttcag ttataaacaa aaactgataa cacgatgtgg acttttcact gctgtgcaaa
     2461 aacgccccga caggtgaaat catctgtgaa ccgacaacct tgagaaaaat cgacacaatg
     2521 cgacgggata cctgaaattg aaccaggaag cattcacaaa tcagttgctc cggcatctcc
     2581 agcaggattt tccactctgc gaaaagcctt ttgcgcagct ggcgaacgaa ctcggcgtag
     2641 atgaaaattc gcttatcgaa caggtcagat gcctcaaaag ctgcggcata atacgcagaa
     2701 taagcggcac attcaacctg aggaaattcg gttatcatat ggctctcgta gccttcgacg
     2761 tcgcgcctga caaaatgcac atcgcggcga acgtgctggt cgaacagcct gccgtcagcc
     2821 actgttacgt gcggcaggat aacgatttcg ccatatggtt cacgctggca ttgcctgaga
     2881 ggctggacga acaggttgcg tcgcttgcga acatttgcaa ggcacgtcga gtgcttaacc
     2941 tgccggctac gcaacatttc aaactttcga cgatgttcga catggagacc ggctgcgtac
     3001 tggaatcgcc cgctgaggcg gaaaaaaccc agccgctcag ccttacgccc gaactgaaga
     3061 aaataatcca cgccctgcag acaagcatca acatttcgca caaaccattt gacggccctg
     3121 caaagactgt cggcgtttcg actgacgaac tcttcagagg cgcagaaatc ctgcgcaagg
     3181 caggcctgct gcggcgatat tcagccgtaa tagaccacgc cgccgccggc attgtcgcaa
     3241 acgcactggt aacatggcag acaaatcccg ttgaagaaga acacgcaggc atactcgcat
     3301 cgctcaatcc gcacgtcagc cactgttacg cgagaaaaac ctgcgacggc tggccgtata
     3361 atctatatac aatgattcat gcgcgaacgc agaaaaaact cgatgaagtc atctgcgaac
     3421 tcgccgcaac cctgggcaac cctccacgca ggatactgcc cacaatgcac gaaatcaaaa
     3481 aagctcctgt actcatatcc taaagcggtg ataaatttcg tatgctttta ccggatatgg
     3541 accggcgttc aagattgttt tattccactt cagagcgttt caaatccgca tcggcggctg
     3601 acggcgtttt cttttgctta tcgcggctgc gacgagcaat atcagaagcg tcgccggttc
     3661 aggtacgagc gtgttaaaca tccataccgt tccgtaaccg tcccccgtgc catagccgcc
     3721 gtaagccgtc gtgccgtaaa ttatgccgtc atcccatata atagaacgca tcggcacatt
     3781 gccgtcttcc atcgaatcga aaacgtgcag aacttcaaaa tccgaaccgt cgagtctcat
     3841 gcgatacagc gttccgtatt ccgtgccgcc ccatatctcg ttgccgtgtg caatgccgta
     3901 cagccagccg tcgggagcta tcagcatgga actgttgggt tttgcgccgt cgcctgtctg
     3961 tgcggaaaaa tcgtgcagta ccgtatattc attggtttcg atatttaagc ggaaaactac
     4021 gccttcgtca gccaactcct cggatctttt cggcgttgat tcttcattat cttcagacca
     4081 cacggcatag cccatggttg tgccgtaaac gtaagtgccg ccgtcccaca ccagcgagtc
     4141 gtacggataa ccgcccgatg tgccgccggc gaatgtgtgc actatttcat atgagccgga
     4201 atccagattg tatttaaaaa tattgccgtg attggccgtg tccctgttat cgctgttcat
     4261 gccgtaaaga taaccgttga ttaccgtcac cgtgccgaac ggctgaacgc cgggcgacgt
     4321 catgttatac aaactggtat aagtgccggt tgaaagattg tactgataaa tattgcttgt
     4381 gccgctggcc cagccgcccc atgtcaaccc gtacaatgag ccgttataat acgtaggagc
     4441 tgtgtatgga tacgaacctt cacctgaacc ggtaaaattg tgaatgacgg aaaaagccga
     4501 agacgaatcg attttgaaaa gaacgccgca gccggcactg ccgccatttc tcgcaatgcc
     4561 atatatattc ccgtcgacca gcgtcaggcc attgtaaact tctatgccgt ccgtggaact
     4621 cgtgaaactg tgaagaactt caaagtccga cccatccgta ttcagcttat ataccgttcc
     4681 gccgccggaa gatgacgaga ctcccacagt cgtgccatat aagacgccgt tggataagat
     4741 gaccgaacca tatggcattg tgcttgttac gccatcgaat tgatgtatga tactgaactc
     4801 cccggcaaaa acgggtgata caaaaaacaa cagccccagc caaatacgta aaaatttcat
     4861 aaccttctcc ttaagggaat aatatacagt agaattttgc ggcttgcaaa tatttttttg
     4921 gggaaagtta gggtgatttg tctgcgcata aaaaatgccc gggactggat tcgaaccagc
     4981 acgaagttaa cctccaccag gccctcaacc tggcgcgtct gccaattccg ccacccgggc
     5041 gtttttcgca tatcgaatga aaaatgcgag acgtaacttt agtcatcgtc cggctgcctg
     5101 tcaagcccaa aaccgccgga atgccccctt tttttcgcaa tgtcatttcg caaaaacagt
     5161 tgcccgcagg cggtgttgta tgataacctt actgtcatgt tgaagaaaac cattctcggc
     5221 gacctgcacg aaaagtcagc ccgcatgggc gaatccggcg gctgggccgt gccgcttgaa
     5281 ttccatggcc agggagacgc agaatccata ctatccggcg gcgtggggat tttcgattta
     5341 tcgcattatt cgcggctgcg gctgcggggt tattccgtgc cagatatgtt cgacgccgtc
     5401 ggcgtagcag gcttggccag catggatgac aacaccgtcc gtgaaatcaa tatcgcagat
     5461 acgcggtgta cgcttgtacg gctggaagat ttttgggtcg tcgtcgcgcc gccggatgca
     5521 aacgagcttc tactggaaaa attccgctct gccgacggcg taaaagctga ttcccagacc
     5581 atgaaagtcg caatgttcgc acttgcaggc acaacggctc ggcagacgct cgcaggagtt
     5641 atgcctgtca aattgccgtc gctggcgaat ggccaggcga tatcgggcag ccttatgctg
     5701 gcaaactata tcgcgtatcg aaacgacgtc gcaggcgtgc cggtcatcaa cgtgatgttg
     5761 ccggcaatat tcgcctcaaa agcatggaat ttcatcacga tgaacgccgg ccggaattgt
     5821 gctatgccgc tcggtatcga cgtatggaat agacttgctt ttaaaaattt ttttaaaatg
     5881 acctcagttt gctggcgggc tttttaaaaa aaattcgacg ggtcgcatat aaaagaatca
     5941 aaaagcagat gcatgaaata ggagcataaa taatggaaca ggcgcagatg gggcagtttg
     6001 ccgatgaatt ttcaaacgac acatggcgga ttttccgcat tataagtgag tttgccgacg
     6061 gttatgagga gatgagcaag cttgggccgg ctgtcagcgt gttcggttca gcccgcatat
     6121 cgcagaacga ccctgattac aagaaggctc gccagtttac cgccatggtg gccaagcgtg
     6181 gattttccat aatcaccggc g
//
LOCUS       DAONFI010000114         6193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14856_length_6193_cov_3.930108, whole genome shotgun sequence.
ACCESSION   DAONFI010000114 DAONFI010000000
VERSION     DAONFI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14856_length_6193_cov_3.930108"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..726
                     /locus_tag="PLK08_04900"
     CDS             67..726
                     /locus_tag="PLK08_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HPS52873.1"
                     /translation="MKNSVLELMVVLSIVFTVASQVQANLVVNGSFEDPVVPTEEWPP
                     DPLVPSDEYPSLPGWTIVSGNFNLCGINSWASAVGNQNLDLAGNTNGSLMQNITTVVG
                     QEYTLSFYYSDNPYMSVAGVASVTVMDNSSEVLLVDETVTGPGNNSPTDMEWKQFSIN
                     FIATSTSISLTFTSLSNPDLNEASPTLDGVVLEAVPEPVTLALLAGGGVLIFAVKRRR
                     R"
     gene            complement(942..1118)
                     /locus_tag="PLK08_04905"
     CDS             complement(942..1118)
                     /locus_tag="PLK08_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52874.1"
                     /translation="MTENIVEVKPEANVYTVLMIITIIVLLVAIAIVGMKLTGSMPNG
                     YGMSIGDMFSPITK"
     gene            complement(1140..1994)
                     /locus_tag="PLK08_04910"
     CDS             complement(1140..1994)
                     /locus_tag="PLK08_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52875.1"
                     /translation="MNILTKICVVLLLVASLVASVVFISIATVGPNWRAKYDEKVTEL
                     QTVKNQLQEASQRLASVENTGKQLRDEKSKLEESSRSKIQAMQAEVDSLKAANSSLAV
                     SYKGVETKLNQISATSDRFATELESLRKKLEESERAKQDLVGKLAEVQNQLVDEQSRQ
                     ARLQDSLKEEKNTSADYKRTIEDLNAKLAVMEKVTGKKASDISTIVSPATRVDGKVTD
                     VSSNLVSINVGSANGLRSGMTLIVYRGDKLVGMLKLQEVSANQSVGVIEEKQSTPMNG
                     DMVTSSLE"
     gene            complement(2225..3439)
                     /locus_tag="PLK08_04915"
     CDS             complement(2225..3439)
                     /locus_tag="PLK08_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS52876.1"
                     /translation="MMKKSQMIFGKIKKTRRHRFFAVILSAWFAGVLFSGCESVMQDD
                     TAVPTVVNSQKARLDARAVLLQATANPSPEVRAHAMEAIGNTLGASEGSYLEQGLRDE
                     SSMVQFAAAMSIGDIRYTPARNILANIVEYPESDKRVVCASIYALYRIGVPKYAPLLA
                     KSLTSDKEQIRSCGAMVLGKMGDTSAIEPLKNLLREETSTATRFNIIQAMAELGDIRS
                     MKMLASFLQEYFLDMKLAAVPAITECHVPNADLILRAQLRETNPPRVRVSAAGALGKM
                     GQSDPKMYEYCVECIRNPQEILRNHYGEKYPMKQVDVDSLVRLSVMAIGDMRDRRGLP
                     MLNQMLKNPDGSLRVTAAMSILHILEPAKSSENTADETATPDTATPEDRKLPPYLPKL
                     FSSDGLDDFPDE"
     gene            complement(3465..4088)
                     /locus_tag="PLK08_04920"
     CDS             complement(3465..4088)
                     /locus_tag="PLK08_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52877.1"
                     /translation="MPFSEYVIGKYSCKPLYDICRSFVPAVMPQLSPGKELARFHISD
                     AAGSWQLATPICFEGTFSRICRRIADSGVKSNLVLANISNDGWFVWKVGEKYVATTEH
                     SQHLVHYVFRAIENRLPVVRAVNTGISGYIDSDGRIRYLPGGVEKFNSSVMLRAVDAV
                     PTLVDSRRSIYTVYGDFFPLSAAAVALAGVLWYFAKRSLRRSKGYGS"
     gene            complement(5096..5518)
                     /locus_tag="PLK08_04925"
                     /pseudo
     CDS             complement(5096..5518)
                     /locus_tag="PLK08_04925"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5750..>6193
                     /locus_tag="PLK08_04930"
     CDS             5750..>6193
                     /locus_tag="PLK08_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52878.1"
                     /translation="MISIKEHVAGHRRSLRAATLVIVLFLCTGIAWLLVAYKSSDSKA
                     AATILSEIRAGGLRKYLSSTRSETCHITVDADGRPLGWKVAYTQPGKNGGYSGGAIMS
                     FNMPDGSAEIAVSRWELDKNATAGRYMSHVFVRDALPQEIDTTEII"
BASE COUNT         1555 a         1669 c         1457 g         1512 t
ORIGIN      
        1 gccagccgac gccgcaaaca gcaacaagcg caaagtcaag tttaatatgc tctaggagcc
       61 tttgagatga aaaatagtgt tttggaactt atggtcgttt tgtcgatagt gtttacggta
      121 gcctcgcagg tgcaagcaaa tcttgttgtt aacggtagct ttgaagaccc ggttgttcca
      181 acagaagaat ggccgcccga cccgcttgtg ccttcggacg aatatcccag tttacctggt
      241 tggacaatag tttctggcaa cttcaacctt tgcggcataa attcctgggc atccgccgta
      301 gggaatcaga acctcgacct tgcaggtaat acgaatgggt cattaatgca aaatattaca
      361 actgtcgtag ggcaggaata tacgctgagc ttttattatt ccgacaatcc gtatatgtct
      421 gtcgccggtg tggcaagcgt tacagtcatg gacaacagtt cggaggtttt attggtagat
      481 gaaactgtta cgggtccagg taataatagc ccgactgaca tggaatggaa acagttttcc
      541 atcaatttca ttgcgacgtc gacatcgatt tctcttacat tcacatccct tagcaatcca
      601 gatttgaatg aagcatctcc gacccttgac ggtgtggttc ttgaagctgt tcctgaaccc
      661 gttacgcttg cactgctggc cggcggaggc gtacttattt tcgccgtgaa aagacgacgc
      721 agataaaact ttacatacgc aataatccca aagattggca gccgattttc agtttgtcag
      781 tttttatacg cggcattaaa ttcagttata gcggttcttg aaagtcttgc aatatcctga
      841 tagataggac ttttatcatc aggacccggt aaattttata ccttgcgtta tttttaaaaa
      901 ccgttataac ccatgaaaat tcatttcagc ttacattcag attatttcgt aatcggcgaa
      961 aacatgtcgc cgatactcat gccgtaaccg ttcggcatac tgcccgtgag tttcatgccc
     1021 actatcgcaa tcgccaccag aagcactatg atggtgatta tcatcagcac cgtataaaca
     1081 ttcgcttcag gttttacttc cactatattt tcagtcattt ccagacctcg ttttcgtaat
     1141 cattcaaggc tcgacgtaac catatcgcca ttcatgggtg tgctctgttt ttcttcaatc
     1201 acgcccacgg actgatttgc cgagacttcc tggagcttga gcatgccgac aagtttatcg
     1261 ccgcgataga caatcagtgt catgccgctt ctcaagccgt tggcagaccc gacgtttata
     1321 cttaccagat tactgctgac atctgtcacc ttgccgtcca cccgcgtcgc cggcgaaacg
     1381 atggttgaaa tatccgacgc cttcttgccg gtaactttct ccatcacagc cagcttggca
     1441 ttgaggtctt caatggtacg tttgtaatct gcggaagtgt ttttttcctc tttcagggaa
     1501 tcctgcaaac gcgcctgacg cgactgttcg tcaacgagct gattctgaac ttcagcaagc
     1561 ttgccgacga ggtcctgctt ggctctttcg gattcttcaa gttttttgcg gaggctttcc
     1621 agttccgttg caaagcggtc acttgtggcg cttatctggt tgagcttggt ttcaacaccc
     1681 ttgtatgaca ccgccaacga agaattggca gccttgagcg aatcaacctc agcctgcatt
     1741 gcctgaattt tagaacgtga agattcttcc agcttgctct tctcgtcgcg caactgcttg
     1801 ccggtatttt ccacgctcgc aagacgctgg ctagcctcct gaagctgatt ttttaccgtc
     1861 tgaagctctg tcactttttc gtcatacttg gctcgccagt tcggcccaac agtagcaatc
     1921 gaaatgaaca cgaccgacgc cacaagcgac gcaaccagca gaagcactac acaaatcttc
     1981 gtcaggatat tcaacgcaga actccttatg taaaaaacaa cttttaacca gcgttccgac
     2041 atcatttacg taggctgatt acagcccaag cctgatatcg gaccgataca cactaaaaaa
     2101 ataaaatatt tacgccgcac tttagcggca gttacaggat aatattggct tttcccgtcc
     2161 ataagtcaag ggaaattata caactaaccc aattttctac gattgtcttt cggggataat
     2221 atctttattc atcagggaaa tcgtccaggc catccgacga aaagagtttg ggaagatatg
     2281 gcggcagttt tctatcttca ggcgttgccg tatccggcgt ggcggtttca tcagccgtgt
     2341 tttcagagga ttttgccggc tcaagtatat ggagtatgga catcgcagcc gtcacgcgca
     2401 atgagccgtc gggatttttc aacatctggt taagcatcgg caaacccctg cggtcgcgca
     2461 tatcgccgat agccatcacc gacagccgca caagcgaatc cacgtcgacc tgcttcatgg
     2521 ggtacttttc gccgtaatga ttgcgaagta tttcctgcgg atttcttatg cattccacgc
     2581 aatattcata cattttcggg tcactttgtc ccattttgcc caacgcgccg gcggcagaga
     2641 cccgcactcg cggcggattg gtttcacgca gctgagccct gagaatcaaa tcggcattgg
     2701 ggacgtgaca ttccgtaatc gcaggcacgg cggctaattt catgtccagg aaatattcct
     2761 gcagaaagct cgccagcatt ttcatgcttc gtatgtcgcc cagctcagcc atcgcctgaa
     2821 tgatgttgaa acgcgtagcc gtgctggtct cctcgcgaag cagattcttg agcggctcga
     2881 tagccgacgt atcgcccatt tttcccaaaa ccatcgcgcc gcaggaacga atctgctcct
     2941 tgtcgctggt aagcgacttg gccagcagcg gagcatactt gggcacacca atgcggtaaa
     3001 gagcgtatat ggatgcacat acgacacgtt tgtcgctttc cggatattcc acgatgttcg
     3061 ccagaatatt cctggccggc gtatagcgta tgtcgcctat ggacatggcc gccgcaaact
     3121 ggaccatgct gctctcatcg cgaaggccct gctcaagata gcttccctcg ctcgcgccga
     3181 gagtattgcc gatagcttcc atggcgtggg cgcgaacttc agggctggga ttcgctgtcg
     3241 cctgaagcag aacggcacgc gcgtccagcc gggctttttg ggaattcacc accgtaggta
     3301 cagctgtatc atcctgcata acactctcgc agccgctgaa cagcacgccg gcaaaccagg
     3361 cagacaggat gaccgcaaaa aatcggtgtc ttcttgtttt tttgattttt ccaaaaatca
     3421 tctgcgattt tttcatcatt taaagaggtt ctcagaaaaa attttcaaga accatagcct
     3481 tttgaacgtc gcaaactgcg cttggcaaaa taccacaata cgccggctaa tgcaaccgcc
     3541 gccgcggata acgggaaaaa atctccataa acggtatata tgctgcgacg ggaatccacg
     3601 agggtgggca cggcatcaac agccctgagc atgacggaac tgttgaattt ttccactccg
     3661 ccgggcagat atcttatgcg tccgtcagaa tctatataac cgcttatgcc ggtattcacc
     3721 gccctgacta ccggcagacg attttctatc gcgcgaaaaa cgtaatgcac aagatgttgc
     3781 gaatgttccg tggtggcgac atatttttcg ccgactttcc acacaaacca gccgtcgttg
     3841 cttatattcg ccaataccag gttggatttg acgcccgaat cggctatgcg acgacagatg
     3901 cggctgaaag ttccttcgaa gcatatcggc gtggcaagct gccatgatcc ggcggcatcg
     3961 gaaatatgaa atctggcaag ctctttacct ggcgaaagtt gcggcatgac tgcgggcacg
     4021 aaactgcggc agatatcgta cagcggcttg catgaatatt tgccgatgac atactcgctg
     4081 aacggcacaa gatggatttt ggaataaatc tcctcctgcc agacgggctg aacagcaata
     4141 cgctgttacg gattacccac tggtcgtcag cctcgattgg ctgcggattg gggtgcagcg
     4201 ttgaaccgcc ggcaattatg tcacagccga gcgatacgga acatgcccgc acgacttcaa
     4261 ccgacacccg caacgatata agctcaaggc ctatgacgtt cggcgacact tttaccaatg
     4321 tacggcggca tatctcggcc agctgcgtcg tatcctgcac atttaacgtc atgacataag
     4381 cgtcaacatg cggctgaaga agttttttaa aatcgtcagg tttgaaattt ttcaggtcgg
     4441 cgttgctaac gtaaagaccg acggctaatc tgcgttgaga tggcgaaagc ggctcagcct
     4501 gcctgcccag cagcatcgcg caaagtttcg caatcctgcc gtcatcgagc gacccgtact
     4561 gctgcgacga aagataattt tccaaaaaat aacgacagcc gtaagacctg ccgtcaatct
     4621 gcgtgatatt gcccaacgcc aacaggagat accggcgaag atatttgacg tcgtgtgcgc
     4681 gggcgatggt tttattgaat atcccgccaa caaggctgac aacttccgca tcgctcagtt
     4741 tcgagacgtt cacgtcgagt acgtccggat tcatacccgc aggcagcatg gtttcgctcc
     4801 agagcaccac gtcgcatcct ttgcctaaaa gttccatgct catccggatg tgtttgtcga
     4861 gcactttgtc cgggccctgg ccggcgtttt cgagcgatat cggttcggcg gactgaacga
     4921 tacccaccac aggcccgcgg ctggtggcat gctgattcat gcgatacccg ccatatatga
     4981 gcatacccgc caatgacgcg acaacaatgg caagaccgca aatcagtttt ttacggctgc
     5041 cgccgccgac tcgccagaca accgcgtcga gcagcaggcc gtcagccatg cccacgaaaa
     5101 acgatacgcc atactgccct gttacgtcag ccacctgtat cagccgtaaa ttctcatact
     5161 gcgtctgaga caggaaaaac cattcaaaac tcaccacatg cgcacgaatg aactccatcg
     5221 tcacgaaaat gaccggcagc cagaaccaca tccatacccg gccgataacg cagcggcgga
     5281 cgacatacga cccggccagc caatacagcg aaagatacgc caccaccgca acgtatccga
     5341 cgccggtcgg caatatcagc cagtatacgc tggcagccca gtatatcgcg ccgcatatcc
     5401 agccaatcag caccgcagcc cgcgtcgacc ttgcaaaacc catcgccgcc gcccacggaa
     5461 caatcgcaat atacgccaga taccacccac caaacggcgc aaacgaaaaa taaagcatcg
     5521 ccatcgacgc catgctcagg ccaagaaccc aataccacgc aaggcagggc gcgactttaa
     5581 ctgtcgttat atgttcatct aaaatatttt ttttcatgtc atccatctgt tcaaatatat
     5641 ccacatcgca aagtaaaggc agtccttatc caggcattgt aaaaaaatat cgtttaaaaa
     5701 tcatcacaat cttgtcggct ggggagatat cggatattct tgttgcgtaa tgatttccat
     5761 aaaagaacat gttgccggac acaggcggtc gctgcgagcg gcgacgttgg tgatagtgct
     5821 gtttttgtgt accggcatcg catggctgct ggtggcgtat aaatcatccg actcgaaggc
     5881 agcagcgacg atattgtcgg aaatccgtgc cggcggcctt cgaaaatatc tttcgtcaac
     5941 cagaagcgag acctgtcata tcacagtcga cgccgacggc cggccgctgg gctggaaagt
     6001 ggcttacact cagccgggga aaaacggcgg ctacagcggc ggagcgataa tgtcgttcaa
     6061 tatgccggac ggctcagccg aaatcgcagt cagccgctgg gaactcgata aaaacgcaac
     6121 tgccggcaga tacatgtcgc atgtattcgt cagggatgca ctgccgcagg aaatcgacac
     6181 gaccgaaatc att
//
LOCUS       DAONFI010000115         6182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14892_length_6182_cov_4.561939, whole genome shotgun sequence.
ACCESSION   DAONFI010000115 DAONFI010000000
VERSION     DAONFI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14892_length_6182_cov_4.561939"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLK08_04935"
     CDS             <1..518
                     /locus_tag="PLK08_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52879.1"
                     /translation="SRLRDFWANRLDDRYPLLMVGLGKMTARNGYVVGKAAPAFVAKI
                     FPGLNLTKYYYQKMTAYQEFQSNGVCVNDAIFSGKSYDVYMNGTTDLDKNIEYTVGLQ
                     LLGSLSSADWKHDWRQGRLPLLKLRGRIEKGKLVDDVMKYPWPNETLGAIFIKNNVFY
                     RAWLLRNQDDQ"
     gene            complement(588..1307)
                     /locus_tag="PLK08_04940"
     CDS             complement(588..1307)
                     /locus_tag="PLK08_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPS52880.1"
                     /translation="MKYLRNGYVVSDLHMFTERTDVNTHMERIFRHADDADFMVLNGD
                     IFDFRWTTLEDFDATTKEAALWLSNVCRRFPKCRFFYVMGNHDGLEHFRGTLDRLSEE
                     MSNFQWNPSHIKIGDCLFLHGDVPMKMGLCDPLERRLLEDETMRGSARQFGYRAIVSS
                     RLHVLSSRLQSPVYAAWRIHKSLVHHHPELAAGLKNVYFGHIHKAFRNFYFRGLWFHN
                     SGSAIKHLECDPINARPNHEQ"
     gene            complement(1317..3155)
                     /gene="typA"
                     /locus_tag="PLK08_04945"
     CDS             complement(1317..3155)
                     /gene="typA"
                     /locus_tag="PLK08_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPS52881.1"
                     /translation="MKIRNVAIIAHVDHGKTTLVDQLLRQSGLFRDSELAVDCIMDSN
                     DIERERGITILSKNCAIDYTARNGERYHINIVDTPGHADFSGEVERVMKMADGALLLV
                     DSAEGVMPQTRYVLSKALEAGLMPIVVINKMDRPDQRAKKVHEEVFDLLVDLGADEHA
                     IDFPFLLASGRNGWAISEEKYKGIPEPGTADIHDLFDAIIEHIPEPNGNPDNPLQMLI
                     TSIDYNDYVGRIGIGRVFEGTIRKGQQITTIMRDGKQIKQKVAILQSFDKLSRKEVDE
                     VTAGNLCAVIGLEHVDIGDTLADLENPVALPPVAIDEPTLHMKFRINDGPFAGRDGKY
                     VTNRQLRERLKRELQSNVALRVEDRGDEFNVSGRGLLHLGILLENMRRESYELCVGKP
                     EVIYRRNENGKLLEPMEKLIVDVPTDGVGPTMQLLGDRKADMLHMDTQGTRSVLEFMI
                     PSRGLIGLRNRLLTATQGEAIIHHRFEKYDEFRGEIGGRLNGVMIATETGQVTAYALD
                     QLADRGIMFVEPGDQVYEGQIVGEHCRDNDLPSNVVRKKNLTNVRSATKDFTVVLKAP
                     RKLSLEQALEYIEDDELVEITPNNVRLRKRYLKEADRKRSFRQAKG"
     gene            3382..6108
                     /gene="alaS"
                     /locus_tag="PLK08_04950"
     CDS             3382..6108
                     /gene="alaS"
                     /locus_tag="PLK08_04950"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPS52882.1"
                     /translation="MARTCKELRAEFVRFFEQKGHRNIISSPLLPADDPTLLFANAGM
                     NQFKPIFLGTETREYSRAVNTQKCIRAGGKHNDLEDVGRDCYHHTFFEMLGNWSFGDY
                     FKKEAIAWAWELLTGVWGIDKTRLHATYFEGDESVFFFKQKTAYEIWKKYLPDDHIHP
                     GNKKDNFWEMGDTGPCGPCTEIHIDLTPDKSGSHLVNAGNPSVMEIWNLVFMQFNRDA
                     AGRLSPLPAKHVDTGMGLERLGMVIQGKRSNYATDLFVPVLEFIETLTAHRYGVASVA
                     GGGDRFDTMNEKDLGDVAMRVIADHARALTFAIADGILPSNDGRGSVLRAILRRAAGF
                     GRQHLKIQGAFLYRIVPVIVKNMGDAFPEIVKRAAYVADVVRDEEESFGRTLDRGLEL
                     FNRHADRILADGGKVVPAEVSFELHATYGFPFSLTKLMAEKRGLSVDENEHIAMMEEH
                     RRASSAGADKFHADAIIGLPETQDDEKYKSTAIDATVLGWVKGDKYITDGFIETGDKA
                     ALVLDRTCFYAECGGQVGDEGTIITTDDGGVVFHVTATKPAGSCVLHFGELLSGRIAA
                     GTLVLAEVDGHRMDTMRNHSATHMLNWALRKVLRDDNVQQAGSVVDSERLRFDFTWPR
                     AVTPEQLAEVEKWVNWKILADQPITVEIIPLDVARKISGVRAVFGEKYPDPVRVVAMG
                     TDDVLGDIDAENCSVEFCGGTHLSRTSQVGMFKILSEESVAKGVRRITAVTGQGAVKW
                     AQRTDVALRSACGVLKTSADELSQRIEAMQLEIKKLRKRPAVAAAAGGETELSGFQTS
                     AGMVVVADAGVCDPAAMRNICDQQRQKGAVAVFLGSADADEGKVTLIAMVDDAVVKAG
                     KAKAGDWVKEIAPIVGGGGGGKPNLAQAGGKHPEKLADALAAAKNWIQKKLG"
BASE COUNT         1335 a         1583 c         1776 g         1488 t
ORIGIN      
        1 acagccgtct gcgggatttt tgggcgaacc gtctcgacga ccgttatccg ctgttgatgg
       61 tgggcttggg caagatgaca gccaggaacg gctatgtcgt gggcaaggct gctccggcgt
      121 tcgttgccaa aatttttccg ggcctgaatc tgacgaaata ttattatcag aaaatgacgg
      181 cgtaccagga gtttcagagc aacggcgttt gcgtgaacga cgcgatattc agcggcaaaa
      241 gctatgacgt ttacatgaac ggcacgaccg acctggacaa gaatatcgag tatacggttg
      301 gccttcagct gctaggctcg ctcagcagtg cggactggaa acacgactgg cggcagggcc
      361 gtctgccgct gctgaaactc aggggcagaa tcgaaaaggg caaactcgtt gacgatgtga
      421 tgaaatatcc gtggccgaat gagactcttg gtgcaatttt tataaaaaac aatgttttct
      481 atcgcgcctg gctgcttaga aaccaggacg accaatgata tgatttttgc gggcatttgc
      541 cgtttgtcat aaaaaatagt gatttgattt tttttgtaca gccggcttta ttgttcgtgg
      601 ttcggccttg cgttgatggg atcgcattcc agatgtttga ttgccgagcc ggaattgtga
      661 aaccacagac ccctgaaata gaaattgcga aaggccttgt gtatatggcc gaaatagacg
      721 tttttcaggc cagccgccag ttcggggtga tgatgcacga gtgatttatg tattcgccac
      781 gccgcgtaaa ccggactttg caggcggctg ctgagtacgt gcagtcgcga tgatacgata
      841 gccctgtatc caaactgtct tgcgctgcct cgcatggttt cgtcctcaag cagcctgcgt
      901 tccagcgggt cgcacaagcc cattttcatc ggtacgtcgc cgtgcaggaa gaggcagtcg
      961 ccgattttga tgtgggacgg attccactgg aaattcgaca tttcttcgct cagtcggtcg
     1021 agtgtgccgc ggaaatgctc aaggccgtcg tgattgccca tgacgtaaaa aaaacggcac
     1081 ttcggaaaac ggcggcagac gttgctcagc cagagggctg cttctttcgt cgtggcgtcg
     1141 aaatcctcca gcgtagtcca gcggaaatcg aaaatatcgc cgttcagcac catgaaatcc
     1201 gcatcgtcgg cgtgtctgaa tatgcgttcc atgtgcgtat tgacgtcagt cctctccgtg
     1261 aacatgtgca ggtcggaaac gacatatcca ttacgcagat atttcatatt ccgtgtttat
     1321 ccctttgcct gtcggaacga acgtttgcgg tcggcttcct tcagatagcg ttttcgaagg
     1381 cgtacgttgt ttggcgttat ttccacgagt tcgtcgtctt cgatatattc cagcgcctgt
     1441 tcgagtgaaa gttttctggg cgctttgagc accacagtga aatccttcgt tgccgaacgt
     1501 acgttggtaa ggtttttctt gcgaaccacg tttgagggca gatcgttgtc gcggcagtgt
     1561 tcgccgacaa tctggccttc gtagacctgg tcgcctggct caacaaacat tataccacgg
     1621 tcggcgagct ggtccagggc gtaagccgtg acctggccgg tttctgtcgc tatcatgacg
     1681 ccgttcaggc ggccgccgat ttccccgcgg aattcgtcgt atttttcgaa tctgtggtga
     1741 attatggctt cgccctgggt ggctgtcaac aggcggtttc gcaggcctat caggccgcgg
     1801 gacggaatca tgaattccag tacgctgcga gtgccctgcg tatccatgtg aagcatgtcg
     1861 gctttgcggt cgccgagtaa ttgcatggtc ggccctacgc cgtcggtcgg gacatccacg
     1921 atgagttttt ccatcggctc aagaagttta ccgttttcgt tgcgacgata aataacttcc
     1981 ggcttgccga cgcagagttc gtaactttcc cgccgcatat tttccagcag gattcccagg
     2041 tgcagaaggc ctctgccgga gacgttgaat tcgtcgcctc tgtcttcgac gcgcagtgcg
     2101 acgttggatt gcaattcgcg tttcagccgt tcgcgtaatt gtcggtttgt cacgtatttg
     2161 ccgtcacggc cggcgaacgg cccgtcgttt atgcggaatt tcatgtgcag cgtgggttcg
     2221 tcgatggcga ccggcggcag agccaccgga ttttccaggt cagccagcgt atcgccgatg
     2281 tccacgtgtt ccagccctat gaccgcgcag agattgccgg ctgttacttc gtcgacttcc
     2341 ttgcgggata gcttgtcgaa actctgcagg atggcgactt tctgttttat ctgcttgccg
     2401 tcccgcatta tggtggttat ctgctggccc ttgcggattg tgccctcgaa gaccctgccg
     2461 atgccgattc gcccgacgta gtcgttgtaa tctatggacg tgatgagcat ttgcaacgga
     2521 ttgtccggat tgccgttggg ttctggaatg tgttcgatta ttgcgtcgaa caggtcatga
     2581 atgtcggctg tgccaggttc gggaatgccc ttgtattttt cctcgctgat agcccagccg
     2641 tttctgcccg atgccagcag aaaaggaaaa tcaatggcat gttcatccgc accgaggtcg
     2701 actagcaggt cgaatacttc ctcgtgaact ttttttgcac gctggtcggg gcgatccatc
     2761 ttgtttatga cgacgatggg cataaggccg gcttccagtg ccttggacag cacgtagcgg
     2821 gtctgcggca tgacgccttc tgcggaatcg accagcagaa gagcgccgtc ggccattttc
     2881 attacgcgct caacctcgcc ggaaaaatcg gcgtggccgg gcgtgtcaac gatatttata
     2941 tggtatcgtt cgccgttgcg ggcggtatag tcaatggcgc agttcttgct gagaatggtt
     3001 atcccacgct cgcgttctat atcgttcgag tccattatgc agtcgaccgc cagttcgcta
     3061 tcgcggaaaa ggccgctctg acgcagaagc tggtccacca gcgtagtctt gccgtggtcg
     3121 acgtgagcta tgattgcaac gttacgtatt ttcatttgtg catcttcttt caacaaaaaa
     3181 acaagggcga gattatatat aactccgtcg ggcttttcca ctaccttaag ataaagtttt
     3241 tacggctgct gtttttattt tgggccttta tgctctctgc ctggctggcg taaatcggtc
     3301 agtttgaagt attttcttga agttgacggg tgttctggta aaattttgcc ctgtttttag
     3361 agatttttca aggaattcat tatggccagg acatgtaaag agcttcgtgc ggaatttgtg
     3421 cgttttttcg agcagaaggg acatcgtaac atcatcagca gcccgctgtt gccggcggac
     3481 gaccctacgc tgcttttcgc caacgccggc atgaaccagt tcaagccgat tttcctcggc
     3541 acagaaaccc gcgaatactc ccgtgccgtc aatacgcaga aatgcatccg agccggcggc
     3601 aagcacaatg acctcgaaga cgtcggccgc gactgttacc atcacacatt ctttgaaatg
     3661 ctcggtaatt ggagttttgg cgattatttc aagaaagagg ctatcgcctg ggcgtgggaa
     3721 ctgcttaccg gagtatgggg catagataaa acccgcctgc atgcgacgta ttttgaaggc
     3781 gacgagtcgg tttttttttt caagcagaag acggcatacg agatctggaa aaaatacctg
     3841 cccgacgacc atattcaccc gggcaacaag aaagacaatt tttgggaaat gggcgatacc
     3901 gggccgtgcg ggccgtgcac tgaaattcac atcgacctta cgccggacaa atccggcagc
     3961 catctggtga acgcaggtaa cccaagcgtt atggagatat ggaatctcgt attcatgcag
     4021 ttcaatcgcg acgcggctgg caggctgtcg ccgctgccgg ctaaacacgt ggataccggc
     4081 atggggctgg agcgtctggg tatggtcatc cagggcaaac gcagcaatta cgcgaccgac
     4141 cttttcgtgc cggttctgga attcatcgaa acgctgacgg ctcaccgtta cggcgttgcg
     4201 agcgtcgccg gcggcggtga caggtttgac acgatgaatg aaaaagacct cggcgacgtg
     4261 gcgatgcgcg tcattgccga ccatgccagg gcgttgacgt tcgcgatagc cgacggcatt
     4321 ttgcccagca acgacggccg cggcagtgtg cttcgcgcca ttctccgtcg ggctgccggg
     4381 ttcggccgcc agcatttgaa aatccagggt gcgtttctgt acagaatagt acccgttatc
     4441 gttaaaaata tgggcgacgc ttttccggaa atcgtcaaac gtgccgctta cgtggcggac
     4501 gtcgtgcgcg atgaggagga atctttcggc cggactctcg acagaggcct ggaactcttc
     4561 aatcgtcacg ccgaccggat attagccgac ggcggaaagg ttgtgcctgc tgaagtgtcg
     4621 ttcgaacttc acgcgacata cggttttccg ttcagcctga cgaaactgat ggctgaaaaa
     4681 cgcggcttga gcgttgatga aaacgaacac atcgccatga tggaagagca tcgccgcgcc
     4741 agcagcgcag gggcggacaa atttcacgcc gatgcaatca tcggcctgcc ggaaacgcag
     4801 gacgacgaaa aatacaagtc gacagccatt gatgcgaccg ttttgggatg ggtcaagggc
     4861 gacaagtaca ttaccgacgg ttttattgaa acaggcgata aggctgcgct ggttttggac
     4921 cgcacctgtt tttatgccga atgcggcggc caggtcggcg acgagggtac gataatcact
     4981 accgatgacg gcggcgtggt ttttcacgtg accgccacaa agccggccgg ctcatgcgtg
     5041 ctgcatttcg gcgaacttct ctccggcaga attgctgccg gcacgctcgt gctggctgaa
     5101 gtcgacggcc accgcatgga tacgatgcga aatcattccg ccacgcatat gctcaactgg
     5161 gcgttgcgga aagtgctcag ggacgacaac gttcagcagg ctggcagtgt tgtcgattcc
     5221 gaacggctgc ggttcgactt cacgtggccc agggccgtta cgccggaaca gcttgccgaa
     5281 gtcgaaaaat gggtcaactg gaaaatcctg gctgaccagc cgataacggt cgaaatcatt
     5341 ccattggacg tagcccgcaa aatttccggc gttcgtgcgg tttttggcga aaaatatccc
     5401 gaccccgtgc gggtggttgc gatgggcact gacgacgtgc tcggcgatat cgacgcggaa
     5461 aattgcagcg tggaattctg cggcggcacg catctttcgc ggacgtcgca ggtcggcatg
     5521 tttaaaatac tttcggaaga atcggtggct aagggcgttc gtcgcataac ggctgtcacc
     5581 ggccagggcg cggtcaaatg ggctcaacgt accgacgtcg ccctccgcag tgcctgcggt
     5641 gtgcttaaaa cgtcagccga tgaactctcg cagcgtatcg aggcgatgca gcttgaaatt
     5701 aaaaaacttc gtaaaaggcc agccgttgct gcggcagccg gcggcgagac ggaattgagc
     5761 ggctttcaga catctgccgg catggttgtc gtagcggatg ccggcgtctg cgacccggct
     5821 gccatgcgga atatctgcga ccaacagcgg cagaaaggcg cggtggctgt ttttctgggc
     5881 tctgccgacg cggatgaagg caaagttacg cttatcgcga tggttgacga cgctgtcgtc
     5941 aaagccggca aggctaaagc cggcgactgg gtgaaagaaa tcgccccgat agtcggcggc
     6001 ggtggcggcg gcaaacccaa cctcgcacaa gccggcggca aacaccctga aaaactcgcc
     6061 gacgcactag ctgcggctaa aaattggatt cagaaaaaac tcggataaat tttagagcat
     6121 gctaaacttg aaattgcgcc ttgttgcgtc gttgaggacg aggctggcaa ggaaggcgaa
     6181 gc
//
LOCUS       DAONFI010000116         6169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14944_length_6169_cov_4.394014, whole genome shotgun sequence.
ACCESSION   DAONFI010000116 DAONFI010000000
VERSION     DAONFI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14944_length_6169_cov_4.394014"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PLK08_04955"
     CDS             complement(<1..529)
                     /locus_tag="PLK08_04955"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS52883.1"
                     /translation="MNENSSNKRERYVIPAVEKAVELLEYLGRDDKGYTQAELVKELN
                     MPLSTCYRIIQTLQKRDWIRKIKDGQYTLSGGMLSAAMNLVNRVARFEAVGPNLKRLA
                     DRTELTCKLSIRQGNQQVTILRAESPRPMSVSGKVGSRFPVIEGSVGAALLCDTPADE
                     ISDIVAACGDDIIEAR"
     gene            797..3385
                     /locus_tag="PLK08_04960"
     CDS             797..3385
                     /locus_tag="PLK08_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPS52884.1"
                     /translation="MEVSIIPFSHLDLFWTGTREQCLSRGNYIISKALDLLEKYDDFR
                     FLIETVNFLDNYLDCHPEDVSRIKKLLKSNRLELASLWAGIYLNMPGGETLARNILLA
                     NDYAGEKLDYKPELAHFGDLPGYTPQYPQIASQADIKYALMSRGGPTESPLFIWESPS
                     GDRIPAYYVTGYATLAMGTIWHRDYESMKSGRLEAILRQKLDGKKHPILFHWGSDLYA
                     PDENIIKNVRRWNEENSIKLNLSTFKEYFFKISKISRMKNAPEIPSLSGELPSSWPNI
                     ESSWPDIWPEDMPCEAALSMAEFLSVFCMCRGWNDYPSELLDNAWKSLLDGMDHNQNG
                     QGGEIADNDKLQLKLWSRYTAEKIIDRMSQRIVAGIRRPHDRAFPIVVFNSMSWRRSE
                     IVVGNIAVFGKVRSCDIHDFENGLQLVDEAGNTVPYIQLSRHEGLSVNMSIAFRAEDV
                     PAAGYKTYYIVPGTNPINESETCCISLDSDIDFSGDAKFFYSTDRSVLIGPRRSCGCN
                     TYENDFFKLTVDCITGEIDVYDVKHEKKLFEKMCIVGVEERRGNYIFDMTASGRAFPA
                     RVDEIETLENNALRCRIQISGEIYGMNFRQAITMFKNSDELEIENVIDWTEPRWIRIE
                     QLFPYAAAEDSSIRYGVPYGYVKYPEIMGGTLGESGDEIDAEDRNRLRLCRHWVDIGD
                     DSSGVTVAADHRMWEFEGQLLRAYMVRGCGYCFGIKRDADGNMENIARPPKGQYTFKY
                     SLRPRYTSLAESNSYRCGWELNNPLHSTGSCETSNIDRYPASDGLLDFTDSSAVVTAI
                     KKSEDGDGLIIRAFEATGNTTKLKIPDLENRIPSQTNILEQIRQPLSERLKPFEIETI
                     VFDKIK"
     gene            3805..4548
                     /locus_tag="PLK08_04965"
     CDS             3805..4548
                     /locus_tag="PLK08_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HPS52885.1"
                     /translation="MPSNIKQREAGLMELDKLISTRQHIPTNEFPIPVKELCERYEKL
                     YTGAINDVMREMCLLDQNLPSDIMPLRDEMTVCGEAFTVKSAPNVMIQGEMTFRAKML
                     DEMKPDGLVVWDTSGDTEGSLWGGVMTATAIAKGLRGAVIAGGIRDTKQILEQEFPVF
                     YKYRTSNGSLGRCLITHYQVPVKVGKVTVRPGDIIFGDIDGVLCVPREIAYDVLVRAE
                     EIESNENEIFKWVRSGETIQEIVDKGGYF"
     gene            4548..5342
                     /locus_tag="PLK08_04970"
     CDS             4548..5342
                     /locus_tag="PLK08_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPS52886.1"
                     /translation="MTNLQLTWLGQSGFVLKTQDTTIACDLYLSDYCQKRSRLDHTRK
                     MQIPIQPEKLEPIDHYLITHSHIDHFDPETVGPVLKSQSATKFYCPPDCKTVRDEFFL
                     ADKSRFTLVNTGCEYNIAQNIRLIPLPAAHEDLAKDNDGEFIAVSYMLLFDELKTAVF
                     FAGDTITYPGQADMIKNALPDDYGLTLVLPVNGRDAKRSALGFKGNLTLNEAVELYRQ
                     CKADKLIPCHFGMFALNDITETMDDAYFTANNCTAIIPEICHPIEL"
     gene            5357..6112
                     /locus_tag="PLK08_04975"
     CDS             5357..6112
                     /locus_tag="PLK08_04975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPS52887.1"
                     /translation="MNIQQLFDLSGRTALVTGSSRGIGKAIAVILAAAGAKIFLHASK
                     SGEKLDAAAKEINAAGGEAIAIAANLSSGGEIDTLIQQIGQLDILVLNASTQFYGTIE
                     SFNADDFQFQYDVNVRSTFKLIKAFLPGMQERNWGRLLSIGSVNEFKPAPRLAVYSST
                     KAAQTNLILNCARQYSQFGITANNLAPGVIMTDRNGKALEDGQFREILLESIPAKRFG
                     RPEDCAAMALLLCSNAGAYITGANIPVTGGMDL"
BASE COUNT         1774 a         1468 c         1410 g         1517 t
ORIGIN      
        1 gcctggcttc gataatgtca tctccgcagg ccgctacaat atcggagatt tcatcagccg
       61 gtgtgtcgca aagcagcgcc gcgcctaccg acccctctat caccggaaat ctgctgccga
      121 ccttgccaga caccgacatc ggcctaggcg attccgcacg caatatcgtc acctgctgat
      181 tgccctggcg aatggataat ttgcacgtca attcagtccg atcagccaga cgcttgagat
      241 tcggcccgac agcctcgaag cgtgctaccc gatttactaa attcattgcc gccgaaagca
      301 tccctccgga cagcgtatat tggccatcct ttatcttacg aatccaatcc cgcttctgaa
      361 gcgtctgaat tatgcgatag caggtactca aaggcatgtt aagctcttta accagctccg
      421 cttgagtata gcctttatcg tcccgcccga gatattcgag caattcgaca gctttctcaa
      481 ccgccggaat aacatatcgc tctctcttat tagaactatt ctcattcata tattaaaata
      541 atatcgttat atttaaacca gtcaagattt ttttattaac tccatacggc aaaattcgtt
      601 tgccattaat agcaataaat cgcattatga taactattgt cgcagttttg ctaattatta
      661 ttcaaaaaga tgctaataaa aacaacttga ccattataaa agatatgcta taatattatt
      721 attacaattt ataatattca tatttaaatc cattaacaac tttatcatat aaggttcact
      781 aatcatgcat gctaaaatgg aagtttctat aatacctttt tcacatcttg atctgttttg
      841 gacgggaaca agagagcaat gcctctcccg tggaaattat atcatttcca aagcccttga
      901 tcttctggaa aaatatgatg atttcaggtt cctcatagaa acggtgaatt ttctggacaa
      961 ctacctcgac tgccatccag aggatgtctc cagaataaag aaactgctga aaagcaatcg
     1021 tcttgaactg gcgtcattat gggcgggaat ctatcttaac atgcccggag gcgaaacgct
     1081 cgcgcggaat atactcctcg ctaatgatta cgcaggtgaa aagctggact acaagcctga
     1141 acttgcacac ttcggagatt taccaggcta cacgccccag tatccgcaga ttgcgtctca
     1201 ggccgatatc aaatacgcgt tgatgtcgcg tggcgggcct acggaatcgc ctttatttat
     1261 atgggaaagt ccaagcggcg acagaattcc ggcatattat gtgacgggat acgcgacgct
     1321 ggcgatgggg actatatggc acagggatta cgagtcaatg aagtcaggcc gcctggaagc
     1381 aattctgcga cagaaacttg acggtaaaaa acatccgatt cttttccatt ggggcagcga
     1441 cctgtacgcc cccgacgaaa acattataaa aaacgttcgc cgctggaatg aagaaaattc
     1501 gataaaatta aatttgtcca catttaagga atattttttt aaaatttcaa aaatttccag
     1561 gatgaaaaat gctccagaaa tcccttctct gagtggcgaa ttgccttcat cgtggccgaa
     1621 cattgaaagc tcatggcctg acatctggcc cgaagatatg ccatgtgaag cggccttgag
     1681 catggcggaa ttcctctccg ttttctgcat gtgcagaggc tggaatgact atccgtccga
     1741 attgttggac aacgcatgga aatctctgct cgacggtatg gatcacaacc agaacggcca
     1801 aggcggagaa attgctgata atgacaaact gcaactgaaa ctctggagcc gttacaccgc
     1861 tgaaaaaatc atagatagaa tgtctcagcg gatagtcgct ggtatccggc ggccgcacga
     1921 cagggcattt ccaatcgtgg ttttcaattc catgtcatgg cgtcgctcgg aaatcgtcgt
     1981 cggaaacatc gccgttttcg gtaaggtgcg atcctgcgac atacacgatt tcgagaatgg
     2041 cttgcagctt gtagatgaag cgggaaatac cgttccatac atacagctaa gccgtcacga
     2101 aggcctttcc gtgaatatgt ccatcgcttt tcgcgcggaa gatgttccag cggctggcta
     2161 taaaacatac tacatcgttc cgggaaccaa tccgataaac gaatcagaaa cctgctgcat
     2221 atccctggac agcgacatcg atttttccgg cgatgccaaa ttcttttact ccaccgaccg
     2281 ctcggttctt ataggcccac gtcgcagctg cggatgcaac acgtatgaaa atgatttctt
     2341 taagttaacc gtggattgca ttacagggga aattgatgtt tacgacgtga aacatgaaaa
     2401 gaaacttttt gaaaaaatgt gtatcgtcgg tgtcgaagag cgcaggggta attatatatt
     2461 cgacatgacg gcaagcggca gagcttttcc agctagggta gatgaaattg aaaccctgga
     2521 aaataatgcc ctgcggtgcc gcattcagat ttccggtgaa atttacggca tgaatttcag
     2581 acaggcgata accatgttca aaaattccga cgaactggaa attgaaaatg tgatcgactg
     2641 gacggagcct cgctggatac gcatagagca actgttccct tatgcagccg cagaagattc
     2701 ctccatccgc tacggcgttc cgtacggata cgtaaaatat ccggaaatca tgggcggaac
     2761 gctcggtgaa agcggagacg aaatcgacgc ggaagacaga aacaggctgc ggctgtgccg
     2821 ccattgggta gatatcggag atgattccag cggagtgacc gttgcggctg accacagaat
     2881 gtgggaattt gaggggcaac tgttgagggc gtacatggtt cgcggctgcg gatattgttt
     2941 cggaattaaa cgcgacgcgg acggaaatat ggagaatatc gccaggccgc ccaaaggaca
     3001 atatacgttt aaatatagtc tcaggcccag atatacttca ctcgccgagt cgaactctta
     3061 tcgttgcggc tgggagctta acaaccctct gcacagcacc gggtcatgcg agacttcaaa
     3121 catcgacaga tatcccgcct cggacggttt actggatttc acagacagtt ccgccgtggt
     3181 aaccgcgata aaaaaatctg aagacggaga cggcctgatt atacgagctt ttgaagcaac
     3241 cggtaatact acaaaattaa aaatccctga tctggaaaat agaattccgt ctcagacaaa
     3301 tatattggaa cagatacgcc agcctctatc cgaacgactt aaaccttttg aaattgaaac
     3361 tatcgttttc gataaaatta aataaaccaa ttgcataaga attttaaatt aacgtcctgc
     3421 ggacgatctt ttgtgttctg tgcccatcat gtggggctga aaatacaaat ccctatacaa
     3481 aatatttaat cgcaaacgca agcgattatt agaatctatc ccaaaaacct tggctgcctg
     3541 caatcggctc ttttgcagcc tggcggcgtc agaaaaacgg caaaatatcc ttaaatatgt
     3601 tgccattttt ctttcttgcc agcccgcaaa attgctcgat ttcggcgacg cccatgtttt
     3661 tgggataggt tctaggaata aaaacttact acaaacatta cgtaagacag atttatctat
     3721 tctgcttttt taaaataaga caaaaaatag gcgaatctgt cttttaaggc agactaatta
     3781 ttaatctctg acaggaataa caccatgcca tctaatatta aacaaagaga agccggcctg
     3841 atggagcttg acaagctcat ctccacacgc cagcacatac cgaccaatga attcccgatt
     3901 ccagtcaagg agctttgcga acgttacgaa aaactctaca ctggcgctat caacgacgtc
     3961 atgcgtgaaa tgtgccttct cgaccagaac ctgccctccg atatcatgcc cctacgcgac
     4021 gagatgaccg tttgcggcga agcctttacg gtgaagagtg ccccgaacgt tatgatacaa
     4081 ggagagatga cattccgtgc taaaatgctt gacgaaatga aacctgatgg ccttgttgta
     4141 tgggatacca gtggtgacac cgagggttca ctatggggcg gcgtaatgac ggccaccgct
     4201 atcgccaaag gcctgcgtgg tgcggtcata gccggcggca ttcgtgacac aaagcagata
     4261 ctcgagcagg agtttccggt cttttataag tatcgaacga gcaatgggtc gctcggcaga
     4321 tgccttatca cgcattacca ggtgccggtc aaggtcggca aggtaacagt ccggccaggt
     4381 gatattattt ttggtgatat cgacggcgta ctttgcgtac cgcgtgaaat cgcgtatgat
     4441 gtactcgtca gggctgagga aattgaaagc aatgaaaacg aaatattcaa atgggttcgc
     4501 agcggcgaga ccatccagga gatcgtagac aaaggcggct atttctaatg acgaatttgc
     4561 aattaacctg gctgggacaa agtggttttg tacttaaaac gcaggatact actatcgcct
     4621 gcgatcttta cctttcggat tactgccaga agcgaagccg gctggatcat acacggaaaa
     4681 tgcagattcc gattcagccg gaaaaactcg aaccgataga ccactatctt ataacacaca
     4741 gccatataga tcatttcgac ccggaaaccg tcggacctgt actaaagtca cagtctgcga
     4801 caaaattcta ctgtccgccg gactgcaaaa cggtaagaga tgaatttttc ttagcggata
     4861 aatcgcgttt cactcttgtc aataccggct gcgaatacaa tattgcgcag aacatcagat
     4921 tgatacccct gccggcagcc catgaagacc ttgccaagga taatgacggt gaattcatag
     4981 ctgtcagtta catgttgctg ttcgatgagt tgaaaacggc agtattcttc gctggcgaca
     5041 ctataaccta tccagggcag gcggatatga taaaaaacgc cttgccggat gattacggac
     5101 taacgttggt cttgccagtc aacggcagag acgcaaaacg gtcagccctc ggattcaagg
     5161 gcaatctaac attgaacgaa gccgtcgaac tgtatcgtca atgcaaggcg gacaaactga
     5221 ttccatgtca tttcggcatg ttcgcattaa acgacattac cgagaccatg gacgatgcat
     5281 attttacggc taataattgt acagcgatta ttcctgaaat ctgccatccg attgagctat
     5341 aattttggag atgaaaatga acatccaaca actgtttgac ctttcaggca gaactgccct
     5401 tgtgacaggt tcgagcagag gcataggcaa agccatcgca gtaatactgg cagcagctgg
     5461 ggctaaaata tttctgcacg caagcaagtc tggtgaaaag ctcgacgccg cggctaaaga
     5521 gataaacgct gccggcggag aagccatcgc gatagcggcc aatctgtcca gcggcggtga
     5581 aatcgacact ctcatccaac agataggcca actcgatata cttgtattga acgcttctac
     5641 acagttctac ggcacaattg aaagtttcaa cgctgacgac tttcagtttc agtacgatgt
     5701 taatgtccgt tcgacgttca aactcatcaa ggcatttttg ccgggtatgc aggagcgcaa
     5761 ctggggccgg ctgttgtcga tcggcagcgt aaatgaattc aagcccgcac ctcggctggc
     5821 ggtttattca tccaccaagg ccgcccagac aaatctgata cttaattgtg cccgccagta
     5881 cagccaattc ggaatcacag ccaacaacct cgcaccggga gtcatcatga ctgacagaaa
     5941 tggaaaagcc ctcgaagatg gccaattccg cgagatactg ttggaatcaa ttcctgcaaa
     6001 acgtttcggc cgcccggaag actgtgccgc catggccctg ctgctatgca gcaacgccgg
     6061 ggcttatata acaggcgcaa atatcccagt taccggcggt atggacttat aaaacaaaag
     6121 ctaaaactta ctttcgccgt caatgtggaa agctggcatc tgtgcatat
//
LOCUS       DAONFI010000117         6155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_14994_length_6155_cov_3.380984, whole genome shotgun sequence.
ACCESSION   DAONFI010000117 DAONFI010000000
VERSION     DAONFI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14994_length_6155_cov_3.380984"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1148)
                     /gene="kdsA"
                     /locus_tag="PLK08_04980"
     CDS             complement(312..1148)
                     /gene="kdsA"
                     /locus_tag="PLK08_04980"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPS52888.1"
                     /translation="MSDKKICQIGNVEFGGGSFSLIAGPCMAESLELCLEVARVMKDL
                     CGELAVGYVFKASFDKANRSSVSSYRGPGIEQGLDWLKTVADKLSLPVLTDIHETCQA
                     ARAAQAVDALQIPAFLARQTDLLVAAGETGKAVNIKKGQFMAPWDMANCAEKVRSTGN
                     SNILLTDRGTFFGYNRLVSDLRCIPQMQQYAPVAFDATHSIQQPGGLGNASGGEREFA
                     PIMGSAAIAAGADALFIETHPNPDNAKSDAGCQIPLAKMRSVLNRCIAVYNAIKNNPV
                     KL"
     gene            complement(1248..2144)
                     /locus_tag="PLK08_04985"
     CDS             complement(1248..2144)
                     /locus_tag="PLK08_04985"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPS52889.1"
                     /translation="MDNVKKPVHLIINPISGYSDQKRRLEIFRKTVFEHGIDLVEHTT
                     TGPGNAGEYALRHAGESIAMVAWGGDGTAKEVANALVGSDTPLMVTHGGTENLLAKVL
                     KMPRDPQMLARLLIDPQLIKFDLGICNGSSFHTVLGVGFDAEVVRLVHENRDGHITHL
                     NYAWPLLKTFINYKFPMLKISADGEDVFYGWGIAFVGNTHRYSMNLRICRDAVCDDGL
                     LDLVVFQCNSKHSLLRHAFWTSMRKHIGRRSVIHQRVKNVHIESDQPQLCELDGDIGP
                     KLPLDISLAEQKINMIVPPWKI"
     gene            2272..3636
                     /locus_tag="PLK08_04990"
     CDS             2272..3636
                     /locus_tag="PLK08_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HPS52890.1"
                     /translation="MTENNQATAVAEDPAKVVDGIIKKDSWTSDDYNELLCQLYAMGD
                     AADKFRTIVNDMETANPNPAGAAAIKIGVSRLLCSRFDDALKALNAATENAEKYYFLG
                     IYYQALSKYADAAKVFAKAIQKGKDTQEVQMLCAESMALSGDMSAAKSTAKKIDSASA
                     DGLYIKGLIAELEGFSQEAFDAYDDAVEMDHSRAMFRLAYFLDLRGNDEEAMDLYQAC
                     LSCPPVYCNTLLNLAILYEDQGKYDDAIRLLNDLLMNNPSHPRAKLYMRDVCASKDMF
                     YDEDKARRIAKRNAVLDIPVTDFELSVRARNCLKKMNIITLGDLVRTSEAELLGYKNF
                     GETSLREIKEMLTAKGLRLGQALEEAQEYNTAGTYTSFSMGDGYVGTAVEGDDSVLSR
                     TIESIEFSVRSRRVLEQLGIRTLGELAARSEIELMSQKNFGQTSLNEMKQKLTEFGLE
                     FRKV"
     gene            3788..5098
                     /locus_tag="PLK08_04995"
     CDS             3788..5098
                     /locus_tag="PLK08_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPS52891.1"
                     /translation="MNSQMHFPGINNGNRRKWRLAVIVAAGVLFVAAGAVLFSYYRHK
                     NVAQLNIEHDSDYLDTNGVPEIRVRLFHNPQKIIEIYTDKPWRVIVDGKDVANGEMPM
                     IPTLCWYVDGKWNLGMLSHDGPEITIEAPGGYVGVDGVTYRGRIVLIADGRDTFWVQN
                     HLDMESYLFSVVSKELYPDFEGDAFRAQAVAARTFAMYESQTRGKTLPYDVWASQRSQ
                     VYGGVPTESDKGRKAVIATRGTVLKYGTPGNEKLFLSQFSACNGGYVNGAEVIRPLQP
                     DEDILPLAGGQADAYGKTCPAYKWKPVKISKQDVFKALAEQYRPVRDMGGLKELQVKT
                     QTPYGRIIWLTVVGVNGKKTQVRAEDLQICLRRSDCQQGRDIMSMNCKMRDLGDSIEF
                     YDGRGFGHGVGLSQWGANERARDGMDYRRILGFYYPGAKLIKEY"
     gene            complement(5345..5599)
                     /locus_tag="PLK08_05000"
     CDS             complement(5345..5599)
                     /locus_tag="PLK08_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52892.1"
                     /translation="MRFIGNHHALHFGGHTFINDVTLINTVIREMINAGKPAAFQEEV
                     TDIITQAVHKVEQELRDELRQKHYHQDLQVQDAAAAAAAK"
     gene            5875..6024
                     /locus_tag="PLK08_05005"
     CDS             5875..6024
                     /locus_tag="PLK08_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52893.1"
                     /translation="MGQRKDRRAYRKKVKAEVKVSNAPRKAKGRIRNAARRAEIEKKL
                     EQAGY"
BASE COUNT         1551 a         1418 c         1634 g         1552 t
ORIGIN      
        1 gcccgcaaaa ttgctcgatt tcggcgacgc ccatgttttt gggataggtt ctagttttat
       61 ctggcgatga aatgtacgcc atttggtgcg tctgtcaggt actttgccaa tatagtactg
      121 gtcagctttt gcataatggc tgattgcgca acagccgggg aatttttaag cctgccgatg
      181 acaacaataa ttggaattca ttcgtcctgt cacacagttc ttttactgac gtgtgtaggg
      241 acgttgcaaa ttacgacggc gaattaattt tcgaacgata tcgatataaa aaataccggt
      301 aacggatttt attatagttt gacggggttg ttttttatcg cattatagac ggcgatgcag
      361 cggttcagaa cgcttcgcat ttttgcaagc ggaatctggc agccggcgtc cgatttcgcg
      421 ttgtcgggat ttggatgcgt ttctataaaa agagcatcgg cgccggcggc tatggcggct
      481 gagcccatga tgggggcgaa ttcgcgttct ccgcccgacg cattgcccag gccgcctggc
      541 tgttgtatgg agtgagtggc gtcgaaggct accggcgcgt actgctgcat ctgcggaatg
      601 catcgcaaat cgctgacaag ccggttatag ccaaaaaaag tcccgcggtc cgtcagcagg
      661 atgttactgt tgcccgtgga gcggactttt tccgcgcagt tggccatgtc ccagggggcc
      721 atgaattggc ctttctttat attgacggct ttgccggttt cgccagccgc taccagcagg
      781 tcggtctgtc gtgcgagaaa tgccggaatt tgcagggcat cgacggcttg ggcggctcgg
      841 gcagcctgac atgtttcatg aatgtcggta aggacgggca gggaaagttt gtcggcgacg
      901 gttttgagcc agtcgaggcc ctgttcgatg cctggcccgc gataactgga aacactggag
      961 cggttggcct tgtcgaagct ggctttgaag acatatccga cggccagttc gccgcagagg
     1021 tctttcatta cgcgggctac ttcgaggcat agctcaaggc tctccgccat gcatgggccg
     1081 gcgatgagcg aaaaacttcc gccgccgaat tcaacattgc ctatttgaca tatttttttg
     1141 tctgacattc agattccttg caaaaatcaa aaataaaatt tatgaaaagt atttccacgg
     1201 tttgaaagcc gtagcatcgt gtcctgaaat cgaaatttaa aaatttttca gattttccac
     1261 ggcggcacaa tcatgtttat tttttgttcg gccagcgaga tatccagcgg cagtttaggc
     1321 ccaatatcgc cgtccaattc acacaactgc ggctggtcgg attcaatgtg cacgtttttt
     1381 actctttggt gaatgacgct gcggcggcca atgtgttttc gcatggaagt ccagaacgcg
     1441 tgccgcagca gcgaatgttt ggaattgcac tggaaaacca ccagatccag caggccgtcg
     1501 tcgcacacgg cgtcgcgaca tatccgcaga ttcatggaat atcgatgggt gtttcccacg
     1561 aaagcaatgc cccagccgta aaatacgtct tctccatcgg ctgatatctt cagcatgggg
     1621 aatttgtagt tgatgaatgt ttttaataac ggccaggcat agttcagatg ggtgatgtgg
     1681 ccgtcgcgat tttcatgaac caaccgaacc acctctgcgt cgaatcccac gcccaacacc
     1741 gtgtgaaatg aactgccgtt gcaaattccc aggtcgaatt ttatcagttg cgggtcgatg
     1801 agcagccgtg caagcatctg cggatctctg ggcattttga ggacttttgc cagcagattt
     1861 tccgtgccgc cgtgcgtgac catcaacggc gtatcgctgc cgacgagggc gttggccacc
     1921 tccttggcag tgccgtcgcc gccccatgcc accatggcga ttgattcgcc ggcatgccgc
     1981 agggcgtatt cgccggcgtt gccaggccct gttgtggtat gctctacaag gtcaattccg
     2041 tgctcgaaaa cagttttgcg gaaaatctcc agccgtctct tctggtcgct gtagccgctt
     2101 atggggttaa ttatcagatg tacaggtttt tttacgttat ccatcttgcc tctcttttta
     2161 cctcgtcggc cggccgtatg caaaaatatt tgcagactga ccttcaatat atataataac
     2221 cgattcaggt tataaggtaa atacctactg gaatattgag agtgatataa aatgacagaa
     2281 aacaatcagg ctaccgcagt tgctgaagac cccgcaaaag ttgtcgacgg aatcataaaa
     2341 aaggattctt ggacgagcga cgattacaat gaattgcttt gtcagcttta cgccatgggc
     2401 gatgctgccg acaaattcag gaccattgtc aatgatatgg agacggctaa tccgaatcca
     2461 gccggcgcag cggctataaa gataggcgtt agccggcttt tgtgcagccg ttttgatgat
     2521 gcactcaaag cattgaatgc agctaccgaa aatgccgaaa aatattattt tcttggcatt
     2581 tattaccagg cgctttccaa gtatgccgat gccgccaaag tatttgcaaa ggcgatacag
     2641 aagggcaagg atactcagga ggtgcagatg ctttgtgccg agtcgatggc gctgagcggc
     2701 gacatgtcgg cggcgaagag tactgccaaa aaaatcgact cagcctcagc agatggcctt
     2761 tacatcaaag gcctcatcgc cgaactggaa ggtttttctc aggaggcttt cgacgcatac
     2821 gacgatgcgg tagagatgga tcattcgcgg gcaatgttcc ggctggcgta tttcctcgac
     2881 ctcagaggca acgatgaaga ggcgatggat ctttatcagg cgtgtctctc gtgtccgccg
     2941 gtctattgca acacgctgtt gaatctggcg attctttatg aagaccaggg caaatatgac
     3001 gacgcaatcc gtctgcttaa cgacctgctc atgaacaatc cgtcccatcc gcgtgccaaa
     3061 ctctacatgc gggacgtctg cgcatccaag gatatgttct acgatgaaga caaggcgcgg
     3121 cggatagcta aacgcaacgc cgttctggat ataccggtga ccgatttcga gttgtcggtt
     3181 cgcgctcgaa actgcctcaa gaaaatgaac atcataacgt tgggcgacct ggtacgcacc
     3241 agtgaagctg aactgctcgg ttacaagaat ttcggcgaga catcactgcg ggaaatcaag
     3301 gaaatgctta ctgccaaggg cttgcgactc ggccaggcgc ttgaggaagc acaggaatat
     3361 aacacagctg gcacttatac gtcattttca atgggtgacg gttatgtcgg cactgccgtc
     3421 gaaggcgacg acagcgttct tagccgtaca atcgaaagca tcgaattttc ggttcgttca
     3481 cgcagagtgc tcgaacagct tggaataagg acgctcggcg aattggcggc acgcagtgaa
     3541 atagagctca tgagccagaa gaatttcggc cagacaagtt tgaatgaaat gaaacagaaa
     3601 cttaccgagt tcggtttgga attccgtaag gtctgaaatc tttggaataa tgaatctctt
     3661 taaatacgac actgcgtcag gtataatgac gacgtgtcgt atttttttga tggatgagtc
     3721 gtgccggcgt tttacttttc tgtgtcgcgt tgacgcgaga aaatgaaaaa tgtgaaaaac
     3781 aggatgcgtg aacagtcaga tgcattttcc aggcataaat aatggtaaca gacgaaaatg
     3841 gcggcttgcc gtcattgtgg cggctggcgt gttgtttgtg gcggctgggg cggttttgtt
     3901 ttcttattac cggcataaga acgtcgctca attaaatatc gaacacgatt ccgattatct
     3961 tgataccaat ggcgtgccgg aaattcgtgt gcgtctgttc cataaccctc aaaaaatcat
     4021 cgaaatttat accgacaagc cgtggcgcgt tatcgtcgac ggcaaagatg tggctaacgg
     4081 tgaaatgccc atgattccaa ctttatgttg gtatgtggac ggcaaatgga atctcggcat
     4141 gctcagccac gatggccctg agattaccat agaagctcct ggcggctatg ttggcgtaga
     4201 cggcgtaaca tatcgcggca ggattgttct tatcgctgac ggccgcgata ctttctgggt
     4261 gcagaaccat cttgatatgg aaagttatct cttcagcgtt gtttcaaaag aattgtatcc
     4321 ggattttgag ggcgatgctt ttcgcgcgca ggctgtggcg gcgaggactt ttgccatgta
     4381 tgagtcgcaa accaggggca agacgctgcc gtatgacgta tgggcgtcgc aaaggtcaca
     4441 ggtttacggc ggcgtgccga cggagagcga caagggcagg aaagcggtta ttgcgacccg
     4501 cggaacagtg ctcaaatatg gtacgcctgg caatgaaaaa ctttttctca gccagttttc
     4561 agcgtgcaac ggcggatatg taaacggcgc cgaggtaatt cggcctcttc agcccgatga
     4621 ggacattctg ccgcttgccg gcggccaggc tgacgcatat gggaaaacat gtccggcata
     4681 caaatggaag ccggtgaaaa tttccaagca ggacgttttc aaggctcttg ccgagcagta
     4741 caggcccgta cgcgacatgg gtgggctgaa ggagttgcaa gttaagaccc aaacgccata
     4801 cggccggata atatggctga cggtggtggg cgtcaacggc aaaaaaacac aggtacgtgc
     4861 cgaagatttg caaatctgcc tgcgacgctc ggattgtcag caaggcaggg atattatgtc
     4921 aatgaactgc aaaatgcggg atttgggcga ttctatagag ttttacgacg gcaggggatt
     4981 cggccatggc gtaggactca gccaatgggg agctaacgaa cgtgccaggg acggcatgga
     5041 ttacaggcgg atactgggat tttattatcc aggcgcgaaa ctcataaaag agtattaaat
     5101 ggtgacgtca gaaaaaagat aagggactta ttgtccaata tacacctgac tcgccatctg
     5161 taaccgcaga tagccgtatc cggttcgaaa atttcaccgc cgttcagtcc aaccccaatg
     5221 ccatagcatt ttgccagata ctctaaaagt ttcggcattc aacgtgtcgg aaaataaaaa
     5281 aagggagtcc ataatattgg attccctttt gtgaaatttg ttttaaaaca ggccgcaaac
     5341 cggattattt tgccgccgcc gctgctgctg catcctgtac ctgcaagtcc tgatgataat
     5401 gtttctgccg cagttcgtca cgcaattcct gctccacctt gtgcacagcc tgcgtaatta
     5461 tgtcggtaac ctcttcctgg aaagctgcag gtttgccggc gttaatcatt tcgcggatta
     5521 cggtgtttat cagcgtcacg tcgtttatga acgtgtggcc gccgaagtgc agagcgtgat
     5581 gattcccgat gaaacgcata agtaatctcc tcgtaattct gtggtttttc cagccagcct
     5641 ccgccagcat tactatattc acattatgcc gatattaacc cgcaggtcaa tagtaatctg
     5701 gcacattaaa atcagcctgc atcccgagct atgaaaaaac gcaaaaaagg tgaatttatg
     5761 ttgcaattag gcgtaaggca caataaaatt cttgccccgt cgggtctgat gtcagcctga
     5821 cgctatgcaa gcagacagta ttgttgatga tacaacttgt gaaaggattc gaaaatggga
     5881 cagcgtaaag atagacgtgc ctatagaaaa aaggtaaaag ctgaagtaaa ggtcagtaat
     5941 gctcctcgca aggctaaggg ccgtatccgt aatgccgccc gcagggctga gattgaaaag
     6001 aagctcgaac aggccggcta ttgagtattt ttaaaatatt ttttaacgaa aaaattactg
     6061 agaaccgtga atttcggcga ttgaatgttg actgaatatt gtaagtacat tgccggcatg
     6121 tgtttcggga gagcctgata tgaatgacgg ccaga
//
LOCUS       DAONFI010000118         6131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_15076_length_6131_cov_4.660303, whole genome shotgun sequence.
ACCESSION   DAONFI010000118 DAONFI010000000
VERSION     DAONFI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15076_length_6131_cov_4.660303"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..2519)
                     /locus_tag="PLK08_05010"
     CDS             complement(174..2519)
                     /locus_tag="PLK08_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPS52894.1"
                     /translation="MKKKTKSAVRQPENCPSAQGQPAAVPGEKLRWVALVILLCVICS
                     RMFIGELPFRIQPVDKAALAGRLQPSEIDGALPPVSMLELARVWYPVAIFFAGGLWLA
                     GELLRRKLKISGIVGGTVAVAFVAMSAASCFYAADRRSAEMVWLEQSAMIFGGFVMMQ
                     LCRCRQRLGMTLVVLAALGATMGIKGLYEKTVEIPDRIAFFQENRERNLQDQGFSPDS
                     SAAKMFEVQLMSTTPGGYLALSNIFGSSLLLLLPAGLTVGCGVISLAVRQKKSGRLEK
                     GQIPFAAPIGVFCLLACVATAMVLIWTRSRGAIGAAAVTVLLIGVLWRFRHALRRRFA
                     TAAIVAVLAVASLVGAVTAYGLKNDSLGITTMTFRWFYWTGGAELIKKHWLTGIGGGN
                     FGDAYLQVRRPEAEEETKTPHNYIIHSLCQFGVFAGGAYVILVGLTVVGAWRSVVKAG
                     GRLEFYLSPSKLGELMFILMLSAVLSRWYFAGMHGILLLVDAIFPAILFGVMIWAFAV
                     QFRSLPMWACSLALAAGVSAFVLHNMVTYSLWSPGTAALFWLTAGAACAAGGRAVEFR
                     KKTVAAAICIWAVMCVFVVAWIVRPVTMRMFFMQKLGEKLCNRDVDWRTAEKAAAADP
                     LDYIAARNVASQIMMELFRNGKTDAGYYRYQQDVLPWLKESQRRNPQNSQSAYYVALL
                     ESHFATSGGFDEFSLSIDRAEKLNPSDADFILRLAVLCNIAGRHEKQRELIAHAKELN
                     DRLKKFSPYSTAILPQERFEEASRESDSEKAKQALFPFVIE"
     gene            complement(2516..3607)
                     /locus_tag="PLK08_05015"
     CDS             complement(2516..3607)
                     /locus_tag="PLK08_05015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPS52895.1"
                     /translation="MLTVGIISAVVAFVISAALTPLCRFVASRGGLLDKPSAVGHKGH
                     EKAVPLLGGAAIFWAIFLPTLAVVAACGVFERTGAPSFLPRYIAVHIHGVVVKTPHVL
                     VIFAAALLLHVVGIIDDRRALGAWPKLAVQFIAATAVVIYGDVRVLTLAGPWVSVPLT
                     IVWIVVITNSFNFLDNMDGLSVGIGAIVAAALALAAWGMGQWFVAAWVCIFFGAFLGF
                     LPYNFAPASVFMGDAGSLVTGFLLAVASCLTTYIVPSSHGGCSAAVAVPLLLMAVPLY
                     DTFSVMYIRLSEGRNPMVGDRRHFSHRLVSRGMSTRSAVLTIYLCAVSTALAGSILPH
                     TRGILPAIMLVSQTICTLLILAMLESRSK"
     gene            complement(3607..4149)
                     /locus_tag="PLK08_05020"
     CDS             complement(3607..4149)
                     /locus_tag="PLK08_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPS52896.1"
                     /translation="MAEIFQPQPVKLICAIIARNEDYAAKAAAELENLYGPADVVSES
                     FPFDMTDYYENQMGSGLLRKFLAFERLIDPSGLADIKRATNDIEKKLAVGADVPRPVN
                     LDPGYVALSRLVLASMKDFSHRIYLGGGVYGEITLMYRGGWKTLEWTFPDFASGRYFD
                     FLTRSRNRLSAAIRAKRSEK"
     gene            4358..5629
                     /gene="serS"
                     /locus_tag="PLK08_05025"
     CDS             4358..5629
                     /gene="serS"
                     /locus_tag="PLK08_05025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPS52897.1"
                     /translation="MIDIKLLRENPDMFRQAAADKNMKVDIDALLNVDAEIRQYKTKL
                     QDVKTAQNEAGKSIAKLPAEEKSAVIAEMARLKEQARQYEETLERLEPEFHELMLLMP
                     QAPHPDAPRGKDDTQNVEVKRVGKVREFDFQPKDHVTLGEELDIIDIPRAVKLAGSRN
                     FILKGAGCLLHQAVLRLALDMMVGRGYTPMTVPVLVNDSVMEGTGYFPLGREQAYLCE
                     RDGQALVGTAEVPVTAYHSDEILSEAELPKKYVAMSTCFRREAGAAGKDTRGLYRIHF
                     FDKVEQVILCKADPQESARMHEEIIKNSEDLLQTLELPYRILEVCTGDMGQGKVRMYD
                     IETWMPSRKSYGETHSASRFHDFQARRLNLRYKDENGKNQFVHTLNNTVVASPRILIP
                     ILELNQNADGSVTIPKALRPYMGGMEKIVKK"
     gene            complement(5868..>6131)
                     /locus_tag="PLK08_05030"
     CDS             complement(5868..>6131)
                     /locus_tag="PLK08_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52898.1"
                     /translation="RRRNRAILLAGKEEKWQHTFKKNIFCRFSDAARLQKSRLQAAKV
                     FGIGSIIGSFAGPLLAVMLMSVWKYKFSPRSIICGLIFGSIAG"
BASE COUNT         1657 a         1799 c         1429 g         1246 t
ORIGIN      
        1 tattttagaa tagcatagct caaataccca tgtcaagcgg ctaactgtat tttattaaaa
       61 atttttgaca aaattttttt aataaaattt tgtcaatgcg ttcttcagga ttccataagt
      121 tcaaatcgca gacgacaaat atcggcggca tacagccgac atcaaaccgc gtattactcg
      181 ataacaaacg gaaacaatgc ttgtttcgcc ttttcggagt cactctcgcg agaagcttcc
      241 tcaaaacgtt cctgagggag tatcgcagtc gaatatggcg agaatttttt taatctgtcg
      301 ttcagttctt ttgcgtgggc aatcaattcc ctctgttttt catgtcggcc ggcgatattg
      361 cacagcaccg ccaacctgag gatgaaatcc gcgtcggagg gattcagctt ttcagccctg
      421 tcgatagaaa ggctgaattc atcaaaaccg cccgatgtgg caaaatggct ttccagcagc
      481 gcaacataat atgcactttg agaattctgc ggattgcggc gttgcgattc tttcagccat
      541 ggcagcacat cctgttgata cctgtaatat ccggcatcgg ttttgccgtt cctgaacaat
      601 tccatcatta tttgcgaagc tacgttgcgt gccgcgatat aatccagcgg gtcggcggcg
      661 gcagcctttt cggcagtccg ccaatcgacg tcgcgattgc ataatttttc ccccagtttt
      721 tgcataaaaa acatccgcat cgtaaccggc cggactatcc acgcaactac gaacacgcac
      781 atgaccgccc atatgcagat tgcggcggcg acggtttttt tgcggaactc tacagccctg
      841 ccgccggcgg cacaggctgc tccagccgtc agccagaaca gcgccgccgt ccccggcgac
      901 cagagactat acgtaaccat attgtgcagc acgaacgcgc tgacgccagc cgcaagtgcc
      961 agcgagcagg cccacatcgg cagactgcgg aactgcacgg cgaaagccca tatcatcacg
     1021 ccgaacagaa tcgccggaaa aatcgcgtct acgagcagca gaatcccgtg catgccggca
     1081 aagtaccagc gggacaatac tgcgctcagc atcaatatga acatcaactc tcccagcttc
     1141 gatgggctga gataaaattc aagtcggccg ccagccttta caacgctgcg ccacgccccc
     1201 acaaccgtga gccccacaag aatcacatac gctccgccgg caaatacgcc aaactgacac
     1261 aacgagtgta ttatgtagtt atgcggcgtt tttgtttcct cttccgcctc aggccgacga
     1321 acctgcaaat atgcatcgcc gaaattcccg ccgccgatgc cggtcagcca gtgttttttt
     1381 atcaactccg ctccaccagt ccagtaaaac catcggaacg tcatggtagt aattcccagc
     1441 gagtcattct tcaacccgta tgccgtcacc gctccaacca gcgacgcaac cgccaacacc
     1501 gccacaatcg cggcggttgc gaacctgcgc cgcaaagcat gcctgaaccg ccacaatacc
     1561 ccaattaata gtaccgtcac cgccgccgca ccaattgcgc cgcggcttcg agtccatatc
     1621 aaaaccatcg ccgtagccac acaggctagc aggcagaaaa ctcctatggg cgcggcgaac
     1681 ggaatctgcc ctttttcaag acggccggat ttcttctgtc gaaccgcaag gctaatcact
     1741 ccgcacccaa ccgtcaaacc agccggcagc agcaataaca acgatgaacc gaaaatatta
     1801 ctcaatgcca gatacccgcc aggcgtagtg ctcataagtt gcacttcaaa cattttagcc
     1861 gccgaactgt caggtgaaaa accctggtct tgcaggtttc tttccctgtt ttcctgaaaa
     1921 aacgctattc tgtccggaat ttcgacggtt ttttcataaa gtcctttaat gcccatcgtc
     1981 gctcccagcg cggcgagcac tacaagcgtc atccccagcc gttgcctgca acggcagagc
     2041 tgcatcatta cgaaaccgcc gaaaatcatt gcggactgtt ccagccacac catttccgca
     2101 cttcgtctgt cggctgcgta aaagcagctc gccgccgaca tcgccacaaa cgctacagca
     2161 actgttccgc cgacgatgcc tgaaattttc agttttcgcc gcaataattc gccagccagc
     2221 cataaaccgc cggcgaaaaa tatcgcgacc ggataccaca cacgagccag ttccagcatt
     2281 gaaaccggcg gcagtgcgcc gtcaatttcc gacggctgaa gtcggccggc gagggcggct
     2341 ttatcgacag gctgaattct gaacggcagt tcgccgataa acattcggct gcatatcacg
     2401 cacaatagaa tcacgagtgc aacccatcgc aatttctcac ctggcacggc cgcaggctgg
     2461 ccttgagccg aggggcaatt ttccggctgc cgaacggcgg atttggtctt ttttttcatt
     2521 tagacctcga ttcgagcatc gccaggataa gcagcgtaca tatcgtctgc gataccagca
     2581 tgattgcggg taaaatgccc cgcgtatgcg gcaggatact gccggcaaga gctgtactga
     2641 ccgcgcagag atatatggtc aaaaccgcac tgcgcgtact catgccgcgg ctgacaagac
     2701 gatgcgaaaa atgtcgcctg tcgccgacca taggattcct gccctcgctc agacggatgt
     2761 acataacgct aaaagtatca tacagcggca cagccatcag cagcagcggc acagccaccg
     2821 ccgccgaaca gccgccatgc gatgacggaa ctatataagt cgtcagacag ctcgccaccg
     2881 ccagcagaaa acccgtcacc agcgagcccg catcgcccat gaaaacggac gccggcgcaa
     2941 agttgtaagg gagaaacccc aaaaaagcac cgaaaaaaat acacacccac gccgcgacga
     3001 accactgccc catcccccac gccgccagcg caagggcagc cgccacaatc gcgcctatcc
     3061 ccaccgacaa gccgtccatg ttatccagaa aattaaacga attcgttatc accacaatcc
     3121 acacaatcgt cagcggcaca ctcacccacg gcccggcgag cgtaagcacg cggacgtcgc
     3181 cgtaaatcac caccgcagtc gcagcgataa actgcaccgc caacttcggc catgctccaa
     3241 gtgcgcggcg gtcgtctatt atccccacaa cgtgcagcag cagcgcagcg gcgaatatca
     3301 ccagcacatg aggcgttttg acgacaacgc cgtgaatatg cacggctatg taacgcggca
     3361 gaaacgacgg cgccccagta cgttcaaaaa caccgcacgc cgccacaact gccagggtcg
     3421 gcagaaatat cgcccagaat atcgccgccc cgcccagaag cggaacagct ttttcgtgtc
     3481 ccttgtggcc gacggcggaa ggcttgtcca ataaacctcc gcggctggcg acgaatctgc
     3541 acagcggcgt aagagccgca cttatgacga atgcaacgac ggctgatatt attccgacgg
     3601 tcagcattat ttttcactcc gtttcgccct gattgcggcg ctgagccggt tgcggctgcg
     3661 agtaagaaaa tcgaaatatc gccccgacgc aaaatccgga aacgtccact caagcgtctt
     3721 ccagccgccg cgatacatca gcgttatttc gccataaacc cccccgccga gatatattct
     3781 atgcgaaaaa tccttcatgc tcgccagcac taaccggctg agtgcaacgt aaccagggtc
     3841 gagattcacc ggccggggca cgtcggcgcc gacagcaagt tttttttcaa tatcgttcgt
     3901 agcccgcttg atgtcggcta agccggatgg gtctatcagc cgctcgaacg caagaaattt
     3961 ccgcaataac ccgctaccca tctgattctc gtaataatcc gtcatgtcga aagggaacga
     4021 ttcgctgact acgtccgccg gcccatatag attttccagc tcagccgccg ccttcgcagc
     4081 gtaatcttca ttacgggcaa ttatcgcaca tatgagtttc actggttgag gttgaaatat
     4141 ttcggccatg cggtgattat cgacactttt agccgtaaag tcaacgcagt gaataaatct
     4201 atacggttca aaaccaaaca ttataaaatt aggataaaaa atgcaggttc agccgaaaca
     4261 cccccgcaaa gaaagagatt ttcctcgaaa tttcaggact gactttgtaa cataaccggt
     4321 ttgaatgagt ccgctcaaaa atatcaggta accaaaaatg atagatatca aattattgcg
     4381 tgaaaatccg gacatgttcc gtcaggctgc cgccgacaaa aacatgaaag tcgatataga
     4441 cgccctgctg aacgtagacg ctgaaattcg ccagtacaaa acaaaactgc aagacgtcaa
     4501 aaccgcccag aatgaagctg gcaagtccat cgccaaactg ccggcggaag aaaaatcggc
     4561 ggttatcgcc gaaatggccc gtctcaagga acaggccagg caatatgagg aaactctcga
     4621 acggcttgag cctgaatttc acgaactcat gcttcttatg ccgcaggcgc cgcatccgga
     4681 cgccccgcga ggtaaagacg acacgcaaaa cgtggaagtc aaacgggtcg gcaaggttcg
     4741 cgagttcgac ttccagccca aagaccacgt cacgcttggc gaggaactgg atataataga
     4801 tattccccgt gccgtcaagt tagccggcag ccggaatttc atactcaaag gcgcaggctg
     4861 tctgttgcat caggcggtat tacggctggc cctggacatg atggttggca ggggctatac
     4921 gcccatgacc gtgccggtcc tcgtcaacga cagcgtgatg gaaggcacgg gatatttccc
     4981 gttgggcagg gagcaggcat atctctgcga gcgggacggc caagcgctgg tcggcacggc
     5041 tgaagtgcca gttacggcat atcacagcga tgaaatactc tcggaagccg aactgcccaa
     5101 aaaatacgtc gccatgagta cctgcttccg tcgcgaagcc ggcgcagcag gcaaagacac
     5161 gcgcggcctc tatcgcatac attttttcga caaggtcgag caggtcatcc tctgcaaagc
     5221 cgacccgcag gaatcggccc gcatgcatga agaaatcatc aaaaattccg aagacctgct
     5281 gcaaactctg gaactgccct accgcatact tgaagtctgc accggcgata tgggccaggg
     5341 caaagtccgc atgtatgaca tcgaaacgtg gatgccaagc cgcaaatcat acggcgaaac
     5401 gcattccgcc agccgttttc acgacttcca ggcaagacgg ctgaacctgc gatacaagga
     5461 cgaaaacggg aaaaaccagt tcgtacatac gctcaataac accgtagtgg caagcccgcg
     5521 aatacttatc cccatactgg aacttaacca gaacgcggac ggttcggtaa cgattcctaa
     5581 ggcattgcgg ccatacatgg gcggcatgga aaaaatcgta aaaaaataga gcctaccacc
     5641 aaaacatggg catcgcctaa atcgtcgccg gattacggcg aagcaatacc atgccacaag
     5701 gcctcattct taaaaacaat tttgcactat atttaagaac cttatccgac ttcggtattt
     5761 tgcggctgcg gtttccgttt cattgatact atccacgcga ccaatacggt acagatagcg
     5821 gaaacaggtg aagtccataa agctgccaac ttaagataaa ataccgttca tccggcaatc
     5881 gacccaaaaa tcaacccgca gattattgaa cgtggtgaaa atttatactt ccagacagac
     5941 atcagcatta cagcaagaag agggccggca aaagaaccaa taatagaacc tatcccaaaa
     6001 accttggctg cctgcaatcg gctcttttgc agcctggctg cgtcagaaaa acggcagaat
     6061 atgttttttt taaaagtatg ctgccatttt tcttccttgc cagccagcaa aattgctcga
     6121 tttcggcgac g
//
LOCUS       DAONFI010000119         6106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_15156_length_6106_cov_3.827962, whole genome shotgun sequence.
ACCESSION   DAONFI010000119 DAONFI010000000
VERSION     DAONFI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15156_length_6106_cov_3.827962"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..810)
                     /locus_tag="PLK08_05035"
     CDS             complement(115..810)
                     /locus_tag="PLK08_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52899.1"
                     /translation="MEFSSEVKEMCKVARGPKNGPAPIPEEGKWVQAKEIRDISAFTH
                     GVGWCAPQQGACKLTLNVKNGIIEEALVETLGCTGMTHSAAMAAEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRSQSAFSENGLPIGAGLEDLGKGLRSVTGTMFGTSAK
                     GVRYLEIAEGYVTRMALDDNDEVIGYEYVKLGVMMDNIVKKGMEPGEAFKKAFGTYGR
                     FAEGVKFINPRHE"
     gene            complement(1081..1479)
                     /locus_tag="PLK08_05040"
     CDS             complement(1081..1479)
                     /locus_tag="PLK08_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS52900.1"
                     /translation="MVSENPVILVVEDDAEMNELQRELLALHGMDSVPAYSGREAVEK
                     HRSVKPDAILLDVMLPEMDGFAACRAIRQEDNRGVPIIMLTAMDSPECRQKGIEAGAD
                     GYFIKPFDPDEVVENLQILLAESGKTRTDK"
     gene            1639..2169
                     /locus_tag="PLK08_05045"
     CDS             1639..2169
                     /locus_tag="PLK08_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52901.1"
                     /translation="MLFWCTMTENRIKQILLPDIATIRKAVDIYMKHAWITPPGEQFT
                     SQLPPAGDFDPITWIMGEMVERDYMGDEKLPDNLKSAAFRLGNTFYPNMKLRLTRMPN
                     SNEFLFSVDSHDAILQAPEGTPDHAMLEELKAANAQITEAVLAEWDTAGIMTERNYLR
                     LKIQQAKNRKVSENRS"
     gene            2173..3822
                     /gene="nadB"
                     /locus_tag="PLK08_05050"
     CDS             2173..3822
                     /gene="nadB"
                     /locus_tag="PLK08_05050"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPS52902.1"
                     /translation="MITSYLTRRYLVSFSFRRLGHIFTDVLIIGTGCAGMRAALTASA
                     AGEVILTSKCKLPESNTYYAQGGMAAVMDADDCFEHHIEDTLRTGVGLCDEDVVNRCI
                     HEAPVHVRQLQDWGLHFDVGEDGYVSLGREGGHGANRIVHAEGDATGKAIFNTLLEKV
                     RQVNNIKIFEECFVLDLLTDPPAGGEGSRCIGAITYHPRYGMQVIHARQIILASGGAG
                     KLWRETSNPACATADGLAIAFRAGAMLADMEMMQFHPTTLYVAGSSRSLISEAVRGEG
                     ALLLDKDGRRFMPDYHEMAELAPRDVVSQAILDTLAKTAATHVFLDIRPMGGEYFRKR
                     FPGIDRRCREFGIDPVKNLIPVHPAAHYMIGGVKVDLDCHTSLENLLACGEAACTYLH
                     GANRLASNSLLEALVFGHQAGKVAGESLTQNNITPYNEINWKIQPSDSTELDMSDIRN
                     SLRSLMWRNVGVVRFGKHLSEAIDIIDFWGRYVMDKEFFNDPGSWEVQNMLTSARMMA
                     ELAVRRTESRGVHYRSDFPRINEDWARHQKIRRTPEQLVVD"
     gene            complement(3924..4367)
                     /locus_tag="PLK08_05055"
     CDS             complement(3924..4367)
                     /locus_tag="PLK08_05055"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPS52903.1"
                     /translation="MSLVDTIRQRITKAMKERDELTRNILRLVLGEIQTAEAREGSIT
                     EETVVKIIRKLIASNEETLSMTKNPQAAEKLRQENVILKELLPHLWSVDDIVKFLNAD
                     AAAAESVKSAGNDGQATGAAMKFLKTASAPINGKDVSQAVKIIRG"
     gene            4454..5527
                     /locus_tag="PLK08_05060"
     CDS             4454..5527
                     /locus_tag="PLK08_05060"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPS52904.1"
                     /translation="MDPFTHALLGASVQEVGLRQKIGRDAAVAGMLAGILPDGDIVML
                     RFGKFFWADPQIALILGHRGVTHSIAAVVILPLLAAAVWWLIRTKVFHRKIPAACQPV
                     SFGWLYLCCLAAVVAHVLLDACTSYGTMLYWPFSTYRIAWDCVAIIDPLFTLIPAAVL
                     VICGLIRRKNGEFSAASVSRTKKTGRWGLVFCVWYLLFGVALHYSVIERSLPAQNRAD
                     VIQAGAFPVVPSLCEWRVVWETPDGWHVGKVNAFSSKQAKFNYVPRRHATQIDAARKT
                     KFYKTFCGFTGRMLRADVKKRGEMTDVYFHDMRYGFPPDSPEGLWAYRFEYDGNGRLV
                     SHGRVYPAGRRGLLHRIKESFKK"
     gene            complement(5532..>6106)
                     /locus_tag="PLK08_05065"
     CDS             complement(5532..>6106)
                     /locus_tag="PLK08_05065"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPS52905.1"
                     /translation="TAASLLAKNLGNTIGNTQNKTINGEMNFTQLYNKSKMLKKIISR
                     LDRIDGRPDISARMSRCRSRVAMAAIIAAFCGEFIATHIPAAEMPAISCSDKTLHFFA
                     YAMLGALFAICQIIRGVRLRNIMLAGVILFPLYAFFDEITQPIVNRTADYFDWRMDMI
                     GSIGVLLISCLAAVFLGRIAKNVKNHKKRT"
BASE COUNT         1468 a         1624 c         1532 g         1482 t
ORIGIN      
        1 cagatttcgg cgcattcttc aacgctcttt atgccgtatt tttcaagaag cgcggttatc
       61 tgggcaattc ggcggtcata tccttcaaat aaagccattt cagctactcc taaattattc
      121 gtgacgcggg ttgatgaatt ttacgccttc ggcaaaacgg ccgtaagtgc caaaagcctt
      181 cttgaaggct tcgcctggtt ccatgccctt cttgacgatg ttgtccatca tcacgccgag
      241 tttgacgtat tcgtaaccga tgacttcatc gttgtcgtcg agggccatcc tggttacgta
      301 gccttctgcg atttccaggt aacgcacgcc ttttgcgctt gtgccgaaca ttgtgcccgt
      361 aacgctgcga aggcctttgc cgaggtcttc aagccctgcg cctatcggca agccgttttc
      421 actgaatgcc gactggctgc ggccataaac tatctgcaaa aacaactcgc gcatcgcggt
      481 attgatggcg tcacagacga ggtcggtatt gagggcttcg aggatggttt tgcctggcag
      541 gatttcggca gccatcgcag ccgagtgcgt catgcccgtg catccgagcg tttcaaccag
      601 cgcctcttct attattccat ttttaacgtt caacgtaagc ttgcacgcac cctgctgcgg
      661 agcgcaccag cctacgccat gcgtaaatgc cgaaatgtcc ctgatttcct tcgcctgcac
      721 ccatttaccc tcttcgggta tcggagccgg gccgttcttg gggccgcggg cgaccttgca
      781 catctctttg acttccgatg aaaattccat caaaaattct cctgacttca aaaaagtttc
      841 cagaaccaca cccttccagc ctgtccagac cgttatccgg gaggttgtta aaatgggttc
      901 tttaacacac gggataccgg cgaaacagta tacaggcaaa cccccgaatt tcaaaacaat
      961 ttgtacacca tgtaccttat tgaaggcgat tttcaaaaaa ttctgcgtaa atgatgagcg
     1021 caaaaaaaat cccctggcgg ggctattggt cagttacgac atttttgttt ttaatttttt
     1081 ttacttatct gttcttgtct ttccggactc agccagaagt atttggagat tctcgaccac
     1141 ttcgtcgggg tcgaacggtt ttatgaagta accgtcggcg ccggcttcta tgcctttttg
     1201 gcgacactcc ggcgagtcca tcgccgtcag catgataata ggcacgcctc ggttatcttc
     1261 ctgccgaatg gcccggcacg ccgcaaagcc atccatttcc ggaagcataa cgtcaagaag
     1321 aatggcgtcc ggcttgaccg agcggtgttt ttccacagct tccctaccgc tgtaggctgg
     1381 cacggagtcc attccgtgaa gagcgagcaa ctcacgctga agttcattca tttcagcatc
     1441 gtcttcaaca accagaatta cgggattttc tgataccatc aactggacaa ctcattttaa
     1501 gttcgggcgg agaaaatacc cacccatgct acttattaat tacgctatct ttacctaaaa
     1561 aggggctata aaatcaataa tcttcttacc aaaatttaaa aatttttata aaatatgcta
     1621 attcaacccg ttgcgattat gttattctgg tgcaccatga ccgaaaacag gattaaacag
     1681 attttactgc ccgacattgc gacaattcgc aaagccgttg acatatacat gaaacacgca
     1741 tggattaccc cgcccggcga acagtttacg tctcaactgc cgccagccgg cgatttcgac
     1801 ccgattacat ggattatggg cgaaatggtc gagcgggatt acatgggcga tgaaaaatta
     1861 cccgataatc tgaaatcggc ggcttttcgg ctgggcaata cattttatcc gaacatgaag
     1921 ctgcggctta ccagaatgcc aaatagcaat gagttcctct tcagcgtgga cagtcacgac
     1981 gccatcttac aggcgcctga gggcacgcct gaccacgcca tgttggagga actcaaggcc
     2041 gccaatgccc agattacgga ggctgtcctc gcagagtggg acacggcggg cataatgacg
     2101 gagcgcaatt atcttcgtct gaaaattcaa caggcaaaaa accggaaagt ttcggaaaat
     2161 cggtcttgaa tcatgattac ttcatatcta acaaggcgtt atctcgtcag tttctcattt
     2221 cgccggctgg gccatatttt taccgacgta ctgataatcg gcacaggctg cgccggcatg
     2281 agggctgcac tgacagcctc agccgccggt gaagtcatcc tgacctccaa atgcaaactg
     2341 cccgaaagca atacctacta cgcccagggc ggcatggctg ccgtcatgga cgccgacgac
     2401 tgttttgaac atcacatcga agatacgctg cgcaccggcg taggcttgtg cgatgaggac
     2461 gtagtgaatc gctgcattca cgaggcgccc gttcacgtac gacagttaca ggactgggga
     2521 ttgcattttg acgtcggcga agacggttac gtttcattgg gacgcgaggg cggccatgga
     2581 gcaaatcgca tagtccacgc cgagggcgac gccaccggca aggcgatttt caatacgctc
     2641 cttgaaaaag tccgccaggt aaacaacatc aaaatttttg aagaatgttt cgtgcttgac
     2701 ctgcttaccg acccgccagc cggcggagaa ggctcacgtt gtatcggggc gataacatat
     2761 catccgcgat acggcatgca ggtcattcat gcccgccaaa tcattttagc cagcggcgga
     2821 gcgggcaaac tctggcggga gacatcaaat ccggcgtgtg cgacggctga cggcctggcg
     2881 atagcctttc gcgcaggggc catgctcgca gacatggaaa tgatgcagtt tcatccgacg
     2941 acgctgtacg tcgccggctc gtcgcgctcg ctcatcagcg aagccgtccg cggcgaaggc
     3001 gcccttctgc ttgacaagga cggcagacgc ttcatgcccg attatcacga aatggcggaa
     3061 ctcgcaccac gagacgtcgt cagccaggca attctcgaca cgttggcaaa aaccgccgca
     3121 acgcacgttt tcctcgatat ccggccgatg ggcggcgagt atttccgcaa aagatttcca
     3181 ggcatagacc gcagatgcag ggagttcggc atagatcccg taaaaaatct cataccggtt
     3241 catccggcgg cacactatat gataggcggc gtaaaggtgg atttggactg ccatacgtct
     3301 ctggaaaatc ttcttgcctg cggcgaggcg gcgtgtacgt atctgcacgg ggcaaacagg
     3361 ctggcgtcga attcactgct ggaggcgctg gtcttcggcc atcaggccgg caaagtggca
     3421 ggcgaatcgc tgacgcaaaa caacatcacg ccatataacg aaataaactg gaaaatccag
     3481 ccgtccgaca gcaccgaact tgacatgagc gatatccgca attcgctgcg cagcctgatg
     3541 tggcgcaacg tcggcgtagt gcggttcggc aagcatcttt cggaggcaat cgacataatc
     3601 gacttctggg gcagatacgt gatggacaag gaatttttca acgaccccgg cagttgggaa
     3661 gttcagaaca tgcttacgtc ggcgcgaatg atggctgaac tcgccgtccg tcgtacggaa
     3721 tcccgcggcg tacactaccg cagtgatttc ccgcgcataa acgaagactg ggccaggcat
     3781 cagaaaatcc gccgtacgcc cgaacagctt gtcgtcgact agaacaccta tatcaaaacc
     3841 ctggctgcct gcaatcggct cttttgcagc catggcggcg ccagaaaaac ggcagaatat
     3901 tttttaaaaa aaatataaaa aaatcagccg cggataattt ttaccgcttg cgatacgtct
     3961 ttgccgttta tgggggctga ggcggttttg agaaatttca tggctgcgcc ggtagcctgg
     4021 ccgtcgttgc cggctgattt gacactctca gccgcggcgg catcggcatt caaaaatttg
     4081 acgatatcgt ccactgacca cagatgggga agaagttctt ttaatattac gttttcctgg
     4141 cggagttttt cggcagcctg gggatttttc gtcattgaca gcgtttcctc attgctggca
     4201 atgagtttac ggataatttt cacgacagtc tcttcagtaa tagagccctc gcgagcttcg
     4261 gcggtctgaa tttcgcccag taccagccgc aggatgtttc tggtcagttc atcgcgttcc
     4321 ttcatggctt tggtaattcg ctgacgtatc gtatcaacaa gactcatgac aatatccttt
     4381 caaaatgtcg caaaataaag tgattccagt atccgatatt gcggcgagga atgcaacagt
     4441 atagaataac ggcatggatc cgtttacaca tgcattgtta ggcgcatcgg tacaggaagt
     4501 cggcctgcga cagaagatag gccgcgatgc cgcagtagca ggcatgttag ccggcattct
     4561 gcccgacggc gatatcgtca tgctgcgttt cggcaaattt ttttgggccg acccgcaaat
     4621 agccctgatt ctcggccatc gcggcgtgac gcactccata gcagccgtcg taattctgcc
     4681 gctgctggct gccgccgtat ggtggctgat tcgcacaaaa gtttttcacc gcaaaatacc
     4741 ggcagcctgt caaccggttt cattcggctg gctgtacctg tgctgcctgg ctgccgtcgt
     4801 agcacatgta ttactggatg cgtgcaccag ttacggtacg atgctgtact ggccgttttc
     4861 tacgtatcgg attgcctggg attgcgtcgc aatcatcgac ccgctgttta cactcatacc
     4921 tgcggcggta ctcgtcatat gcggccttat ccgaaggaaa aacggcgaat tttccgccgc
     4981 gtccgtcagc cgcacgaaaa aaacgggccg ctggggccta gttttctgcg tgtggtatct
     5041 gcttttcggc gtcgcgctgc actacagcgt aatcgaacgc agcctccctg cacaaaaccg
     5101 tgccgacgtg atacaggcag gcgcatttcc ggtcgttccg tcattgtgcg agtggcgcgt
     5161 ggtttgggaa acgccggacg gctggcacgt cgggaaagtt aatgcgtttt cttctaaaca
     5221 ggcgaagttc aattatgtcc cgcggcggca tgccacacag atagacgccg ccagaaagac
     5281 aaaattttac aaaacattct gcggttttac cggccgtatg ctgcgtgccg acgtaaaaaa
     5341 acgcggcgaa atgaccgacg tttattttca tgacatgcga tatggatttc cgcccgactc
     5401 tccggagggt ttatgggcat acaggtttga atacgacggc aatgggcggc tcgtatctca
     5461 cggccgagtt tacccagccg gcagacgcgg cttgctgcat cgcataaagg aaagttttaa
     5521 aaaataatct tttatgtccg ttttttatgg tttttaacgt tttttgcaat tcgccccaaa
     5581 aatactgctg ccaggcagct tatcagcagt acccctatcg aacctatcat atccatgcgc
     5641 cagtcaaaat aatcggcggt acgattcaca atcggctgcg ttatttcgtc gaaaaatgcg
     5701 tacaacggga aaagtatcac gccagccagc ataatgttcc gcaggcgcac accacgaata
     5761 atctggcata tcgcaaacaa agccccaagc atggcgtagg cgaaaaagtg cagggttttg
     5821 tccgaacagc tgattgccgg catttcggca gcaggtatat gcgtagcaat aaattcaccg
     5881 cagaaggctg ctattatcgc agccatcgcc acgcgtgagc ggcagcgact catcctcgca
     5941 gaaatgtcag gccgcccatc aatcctgtcc agccgggaaa taatcttctt cagcatcttg
     6001 gacttattgt acaactgcgt gaaattcatt tcaccattaa tggttttatt ctgggtattt
     6061 cctatcgtat tccctaaatt ctttgccaat aaggaggcgg cggtcc
//
LOCUS       DAONFI010000120         6090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_15209_length_6090_cov_4.453853, whole genome shotgun sequence.
ACCESSION   DAONFI010000120 DAONFI010000000
VERSION     DAONFI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15209_length_6090_cov_4.453853"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="nrdD"
                     /locus_tag="PLK08_05070"
     CDS             complement(<1..837)
                     /gene="nrdD"
                     /locus_tag="PLK08_05070"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPS52906.1"
                     /translation="MFERIRKRDGSVAGFLPEKISRAIFKAAQACGGNDYVKAEQLCG
                     QVLKIAEGKFGSDAVPEVEKIQDIIEKVLIENGHAQTAKAFILYREKRKGARQFNALV
                     GATIDMFNNYLQDRDWQAKENANTQKSINGLNNYVREFFTKNYWLYEVYPREVREAHE
                     TGAAHIHDLGFLGAYCAGWDLRQLLIDGFGGVPGKCESRPARHLRSFLGQIINSTFTT
                     QGECAGAQAWSSFDTYCAPFIRYDRLTYTQVKQALQEFVFNINVPTRVGFQCPFSNLT
                     FDL"
     gene            complement(1329..1463)
                     /gene="rpmH"
                     /locus_tag="PLK08_05075"
     CDS             complement(1329..1463)
                     /gene="rpmH"
                     /locus_tag="PLK08_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPS52907.1"
                     /translation="MHYPHRISRRKRVRKFGFLARMRSSNGRSIINRRRARKRQATIL
                     "
     gene            1654..2211
                     /gene="cyaB"
                     /locus_tag="PLK08_05080"
     CDS             1654..2211
                     /gene="cyaB"
                     /locus_tag="PLK08_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HPS52908.1"
                     /translation="MPYFSGMKEKELKIPVDSHDKIKARLAGLGAEFLCHVTQKDDFY
                     DTPDGCVMNGDSCLRLRHVVHLDGAADDEYFLTWKGPREDVWAKVRPEYETAVSSPAA
                     VGKILNSLGYEKTFEVVKKRTSYRLGCCRIELDELDRLGFFVEIEGPDEAELEKTRKL
                     LQIEGHEPRSYASLLRQAIKKTKNS"
     gene            complement(2235..2573)
                     /locus_tag="PLK08_05085"
     CDS             complement(2235..2573)
                     /locus_tag="PLK08_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS52909.1"
                     /translation="MKLNTEIKGDVAIVRIDGDHLDASTHAKFVQQLKPVTDQYSKII
                     FDIGSLEFIDSSGIGVILGTMRRARATGGDVKLACPTEAVTSIFNLVRMSRLINIVPT
                     IDEAVAGFGQ"
     gene            complement(2957..5053)
                     /locus_tag="PLK08_05090"
     CDS             complement(2957..5053)
                     /locus_tag="PLK08_05090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPS52910.1"
                     /translation="MPGIIIIACFAIIIGLLSAALAVVRKRLLAARLDLEAESDARQD
                     SDVMMRQVESQYHALLEGITEGVVIVDSGHRIKMINQTMCDIFVTDPKGIFEQKCFEH
                     LCGSEKPCENCPGIWSIASGKPAVSTLEMHRADGSKLVARIHSMPVKDKNGDLTMFML
                     IVEDITNRHDWETSIRQNERRYRTLFRELGEGFMYIKSENSSNEQSAVSFKILDMNPA
                     MEELLGSNRADMIDAEITQTPLGKIQEFMEHMKAVDSLGSSAQDEIYLPELDKYIAYR
                     IFRPSSMRIAVICRETTEYRLAEQQLSEAQERELEFGTRIQQTMLKGRRPEHLCGVEV
                     AVHTIPSYKIDGDFHDFITYDSKHFDVIIGDVMGKGGSAALLGAAAKLEFVRDIGALL
                     SASGNTELSTPEEIVNKVHAHICENLMELSSFVSACYARFNLDKKRLDYVDCGHTAVA
                     HHRSAVGEFSLLHGDNMPLGFSDAEVYAQHSVIFNDGDVFVFYSDGLIQATDAAGNVF
                     GEERLKHHITMNAQMSAEIVCQKLIEAVKTFTGKPSQLDDLTCIVVKIISPKENNVLI
                     RKDMNLRQKLVDLVGLRNFIRACCQESPLTLDEEFISRIELAANETASNIIFNSGESD
                     AAITVIFSIYDSQAVVDIVHSGQMLPEKESTSPALDGSDESGFSLFLIRNCVDKVEYD
                     RTPDGLNRVRLIKYIA"
     gene            5480..>6090
                     /locus_tag="PLK08_05095"
     CDS             5480..>6090
                     /locus_tag="PLK08_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPS52911.1"
                     /translation="MRVACKNLAAIAICVMLSFAAGCYNAKSVNKFLPEPRTPVATPE
                     YRVYPPDLLLISSDNMREFRPELGKRGMQILVMPDGKINVPLIGEIFVAGKTVHEIER
                     DILRAAEKYYSEATVNVDVMRFDSQRYYVMGEVKNPGPKKWTGRDTVLDAVAQAGLTP
                     ESWSERIIIVRGDSPQSGGVEQGSEYDKGYYYRGVRRERPMSP"
BASE COUNT         1557 a         1511 c         1432 g         1590 t
ORIGIN      
        1 caggtcaaac gtaagattgc tgaacgggca ctgaaaacca acgcgggtag gcacgttgat
       61 attgaacacg aattcctgca gcgcctgctt cacctgcgta tatgtgagtc tgtcataccg
      121 gataaagggg gcgcaatacg tatcaaaact gctccatgcc tgagcgccgg cacattcgcc
      181 ctgggtggta aaggtggagt tgattatctg gcccagaaag ctgcgaagat ggcgggctgg
      241 ccgtgattcg cacttgcccg gaacgccgcc aaacccgtct atcagaagct gacgcaaatc
      301 ccagccggca caatacgcgc caagaaatcc aaggtcatga atgtgggctg caccggtttc
      361 gtgggcttca cgaacttcac gaggataaac ttcgtacagc cagtaatttt tggtaaaaaa
      421 ctcgcgaacg taattattca acccgttaat cgatttctgc gtattggcgt tttcctttgc
      481 ctgccagtcg cgatcctgta gataattgtt aaacatgtct atcgtggcgc ctacgagggc
      541 gttgaactgg cgggcgcctt tgcgtttttc acggtagagg atgaacgcct tggctgtctg
      601 ggcatggccg ttctctataa gaactttttc aattatatcc tgaattttct caacttcagg
      661 tacggcgtca gagccgaact tcccttcagc gattttcagc acctggccgc ataactgttc
      721 agccttgacg taatcattgc ccccacaagc ctgagccgcc ttgaaaatgg ctcttgaaat
      781 tttttcgggc aaaaatccgg cgacacttcc gtcacgcttt cgaatccgtt cgaacatcgc
      841 tgactccttt tcacctcccg caggaggaca ctaccaccgt tattattaaa aatataaata
      901 aatcgtatga aaaatatcaa tgtgacccga tgaggatccg atatcgcttt tcgatttcaa
      961 ccataatatg cgcttcgcct ccaaatgtca cgacaaagtc cggattgctc atacttaaat
     1021 atcggcaaga aagaccctgt tgcaacagtg aaacccaata tattgtgtta tacgaaaaaa
     1081 tcaaccccca aaatgtagtt tattttaaaa agtaggtcgc tagccttcgc cgccggcgga
     1141 aggctggaag gtgttgaaaa tcaggacgtt atattacgca aaaacgccct acagaaaaat
     1201 tgcaggaaat ttttataaaa atttcatcgt gcgcaaacaa aaagcgtagc gcagaaaatt
     1261 ttctgctgca ctacgcttgt aatttcttaa attttttaaa atcgcttaaa cgattttaaa
     1321 cggtcagatt agagtatggt agcctgacgt ttacgtgcgc gcctgcgatt gatgatgctt
     1381 ctgccgttgc tggaacgcat gcgagccaaa aatccgaatt tgcggactct tttacgcctt
     1441 gaaatacgat gcggataatg cattttacac ctcagcctct tatttttact ttaaaaactt
     1501 tcaaaacact tctaaaatca ccgctaactc tccatcaagc ctgaacttgc cggaggggca
     1561 gggataatac actctatccg ccagtttgac aagaaaattt ttaaggattt tttcaaaaaa
     1621 agcccccatg taatttgaaa aatagcggcc gacatgccat actttagtgg catgaaagaa
     1681 aaagagctta aaatccccgt cgattcgcat gataaaatca aggcccgact agccgggctg
     1741 ggcgcggaat tcctctgcca cgtcacccaa aaggacgatt tttacgatac gcccgacgga
     1801 tgcgtcatga acggcgacag ttgtctgcga ctacggcatg tggtgcatct ggacggggcg
     1861 gctgacgacg aatatttttt aacatggaaa ggcccccgcg aagatgtttg ggccaaagta
     1921 aggcctgaat atgaaacggc ggtctcctcg ccggcggctg tcgggaaaat tctcaattcg
     1981 ttaggctatg aaaaaacttt cgaagtggtc aaaaaacgca ccagctatcg tctgggctgt
     2041 tgcaggattg aactggacga actcgacagg ctcggatttt tcgtggaaat cgaaggcccc
     2101 gacgaagccg aactggaaaa gactcgcaaa ctcttgcaaa tcgaaggcca tgaaccccgt
     2161 tcctatgcct cattacttcg ccaagccatc aaaaaaacca aaaattcctg aatatcgagt
     2221 caaatacaag attgtcattg gccaaaaccg gcgacggctt catctatggt cggaacgata
     2281 tttatcagcc tgctcatcct cacgagattg aatatcgacg tcactgcctc cgtcggacat
     2341 gcaagtttga cgtcgccgcc agttgcacgg gcacgtcgca tcgtgccgag tatgacgcct
     2401 atgccagatg aatctataaa ttcaaggctg ccgatgtcaa atatgatttt tgaatactgg
     2461 tccgtaacag gtttgagctg ttgcacgaat ttggcgtggg tgctggcgtc aagatgatcc
     2521 ccgtcaatcc tgacgatagc tacgtcacct tttatttcag tgtttagctt catatctttt
     2581 ccttatccac aaagtaaaaa ttctatggct taaaaccgta gcatcgtatt tatcaggcta
     2641 tcgcctgtcc gcctggcggc ggatgccttc atctgcggct ttttttattt taaaaaaacc
     2701 gtagaatcac aaagaacatt ttcaaactga ccgatttaac ggctttcaga gtaccaggcg
     2761 taaagataaa tacttgtttt acatttggtt ctgatttaac ggcaatcatt tgtcaaccta
     2821 tttttgtcaa attgacaatt tgtgtaactt tgtgtaaaac cagtatttac cgaaccaaat
     2881 atcgtcaatc ggtcactttg agtaacattt tctacatacc acaccattat atatcgacag
     2941 cagcaaggaa aacagtttac gcgatatact ttatcaatct gactctgttt aacccgtctg
     3001 gagtcctgtc atattccacc ttatcgacgc aatttcttat gaggaacagg ctgaatcccg
     3061 actcatcact tccgtcgagc gccggcgagg tactttcttt ttcggggagc atctgccccg
     3121 aatgtacgat atccaccaca gcctgcgaat cgtaaatgct gaaaataacc gttatcgcag
     3181 cgtcagattc tccggaattg aatatgatat tgctggcggt ttcgttagcc gccaattcaa
     3241 tgcgggaaat gaattcctcg tcaagcgtca gcggcgactc ctgacagcat gcgcggatga
     3301 aattcctcag cccgacaagg tcaaccagct tctgccgcaa attcatgtcc ttgcggataa
     3361 gaacattgtt ttcttttggg ctgattattt ttacaactat gcaggtcaga tcgtcaagtt
     3421 ggctgggttt gccggtgaag gtttttacgg cttcgatgag tttctggcag acgatttcgg
     3481 ctgacatctg cgcgttcatg gtgatatgat gttttagccg ttcctcgccg aagacattgc
     3541 cggcggcatc cgtcgcctga ataaggccgt cggaataaaa cacgaacaca tcgccgtcgt
     3601 tgaaaattac ggaatgctgg gcataaactt ccgcatccga aaagccgagc ggcatattat
     3661 cgccgtgaag aaggctgaac tccccgacag cgctgcggtg gtgcgcgacg gcggtatggc
     3721 cgcagtcgac gtagtccagc cgttttttat cgaggttgaa cctggcgtaa cacgccgaca
     3781 cgaaactgct gagttccatc aggttttcgc agatatgggc gtggaccttg ttgacgattt
     3841 cctccggcgt ggaaagttcg gtattgccgg acgccgaaag cagtgcgcct atatcccgta
     3901 cgaattcgag cttggcggca gcccccagca gtgcagccga gccgcccttg cccatgacgt
     3961 ctcctattat cacgtcaaaa tgtttggagt catatgtaat gaagtcgtga aaatcgccgt
     4021 cgattttata tgacggaatg gtgtgtacgg ccacctctac gccgcagagg tgctcaggcc
     4081 gccggccttt gagcatcgtc tgctgaatac gggtgccgaa ttcgagttca cgttcctgtg
     4141 cctccgagag ttgctgctcg gcgagccgat attccgtagt ttcacggcat attaccgcaa
     4201 tacgcatgct cgaaggccta aatatccggt aggcgatgta tttatcgagt tccggcagat
     4261 aaatttcatc ctgagccgaa ctgccgagcg aatcaacggc tttcatgtgc tccatgaact
     4321 cctgtatctt ccctagcggc gtctgtgtta tttctgcgtc tatcatgtcg gctcgatttg
     4381 agccgagtaa ctcttccatg gccgggttca tatccaatat tttgaaactg acggcagatt
     4441 gctcatttga agagttttcg gactttatat acatgaaccc ttcgcccaat tccctgaaaa
     4501 gcgttctgta ccgcctttcg ttctgcctga ttgacgtctc ccagtcgtgt cgattggtta
     4561 tatcttccac aatcagcatg aacatggtca agtcgccgtt tttatccttg acgggcattg
     4621 agtgtattct ggcgaccagc tttgaaccgt ccgcgcgatg catttcaaga gtggaaacgg
     4681 caggcttgcc ggatgctatc gaccagattc cagggcaatt ttcgcacggt ttttccgagc
     4741 cgcacagatg ctcaaaacat ttttgctcaa aaatgccctt ggggtcagtg acgaatatgt
     4801 cacacatggt ctggtttatc atctttatac gatgtccgga atcgacgatt acgacccctt
     4861 cggttatgcc ttccagcaag gcgtgatact gcgattccac ctgtcgcatc atcacgtctg
     4921 aatcctgcct ggcgtccgat tcagcctcca ggtccagcct ggctgcaaga agccttttcc
     4981 tgacaaccgc cagcgcagcc gataaaaggc ctataataat cgcaaaacat gcaatgatga
     5041 tgattccggg catcttattc caaaacctcc ggattgccag gctgccggaa ttttctgacg
     5101 tcaacgccga aagcctgacc gtccacaact ttgcggatat atgtttaaat ccacggacgc
     5161 attcgtcttg tttttatatg attcatcaac ggcggttaaa atcacacatc ctcggatgct
     5221 cagccgctcc acattaatgc tatcgccgca tggtatgaaa tgtcaagtgc ttatgaaaaa
     5281 gtattccggc tattaatgaa ttttttttgg gataaaaacg tcaaaaaggc tgagctttat
     5341 tgtctgaaaa tgccgatacg gattaagtgt atccatgtgc tttgggcaca cggaggatat
     5401 ttttgcaaga ctgtcagccc tgaaacacgg ttggtgtaat tgcgtaagga tatgcggata
     5461 tggagtttac gacaatttga tgcgtgttgc ttgtaaaaat ctggctgcga ttgcgatttg
     5521 tgtaatgctg tcattcgcag cgggctgtta taacgcgaaa tccgtcaaca aatttctgcc
     5581 ggagccgcgt acccctgtgg ctacgcctga atatcgcgta tatccgcccg acctgctgct
     5641 catcagttcc gacaacatgc gggaatttcg gccggaactg ggcaagcgcg gcatgcagat
     5701 actcgtcatg cccgacggca agataaatgt gccgctcatc ggcgaaatat tcgtcgccgg
     5761 caaaaccgtg catgaaatag agcgtgacat tctgcgggct gcggagaaat actactccga
     5821 agccactgtt aacgtcgacg tcatgcgttt tgactcccag cgatactacg tcatgggtga
     5881 ggttaaaaat cccggcccaa aaaagtggac cggtcgcgat accgtcctcg acgccgtcgc
     5941 gcaagccggc ctgacgccgg agtcatggtc tgagcgtata ataatcgtac gcggcgactc
     6001 gccgcagtcc ggcggagtgg agcagggcag cgaatacgat aagggatatt attacagagg
     6061 cgtccgcagg gaacggccaa tgtcgccgag
//
LOCUS       DAONFI010000121         6069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_15282_length_6069_cov_3.735451, whole genome shotgun sequence.
ACCESSION   DAONFI010000121 DAONFI010000000
VERSION     DAONFI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15282_length_6069_cov_3.735451"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLK08_05100"
     CDS             <1..401
                     /locus_tag="PLK08_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52912.1"
                     /translation="YILAGAAGDYFKATLDGLELFSSGPGELICESWPRETIRRGFSG
                     LCGEILLDMGRRSRRITQRGFLRAQTVEQLYLQTAAIESMADGMAHLLIDDDGVTYQK
                     VIMEKFEKPDAINKSDICSCEYEIIYRQLP"
     gene            398..1132
                     /locus_tag="PLK08_05105"
     CDS             398..1132
                     /locus_tag="PLK08_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52913.1"
                     /translation="MSEIQHIQVDKLWIVETSTPSVLEVRFIAPAGAEEDWHYQLYLN
                     GTWADTSESSNQRAFSVHAQKYPQMFAVFAVEPAGRNVNLSALLPEDLRTPSWVCNFR
                     MTQDTAFEPGDRMEIYHDDGSGTMDEQPALRWLAWPPAISHTGWGNATLLNSGFGIDG
                     SNAPGMSAPFGIGPMGLGSFELKTVLPLKNSGHHNIRVLCRSSDGIASKSKALTFFSS
                     APPSRPGKITFISHNPSTTIAKFRIR"
     gene            1145..2071
                     /locus_tag="PLK08_05110"
     CDS             1145..2071
                     /locus_tag="PLK08_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52914.1"
                     /translation="MSELYPSDAIINALSGTKDSGQEVQYPAIYETPYYTTFYKMIYR
                     LLNVARRAGDFRIYKDGDLTFGVRAGKVSLGNSRFDFSGAAQQVLTDNATNSIYLTVD
                     GVVHISTSGFPSVSTTAFIPLAIISTANGSYDFEDITDLRALSMFQFNTNLTPDNAQI
                     LTTSKVADCLHTHDSAGIANEALGGVHLNSQLRKSCSYAVLTTTDNANGTATITISIR
                     YASGTSSTNLNLVRIWTSDSSLTIPSEQATLTIQQGLVMNTYQANVDLEILTAQTGNI
                     VMLASSSANKSFHVHVEFRGIVDVYSVSITGN"
     gene            2073..2282
                     /locus_tag="PLK08_05115"
     CDS             2073..2282
                     /locus_tag="PLK08_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52915.1"
                     /translation="MEISFTANDVIYVAHYVGARDGKAGRWWQCDELDLWLQPPVNTE
                     ANELPTLFAEAILQQEISDGFAAAL"
     gene            2260..4335
                     /locus_tag="PLK08_05120"
     CDS             2260..4335
                     /locus_tag="PLK08_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52916.1"
                     /translation="MALLLHYEFDTADSLDGWTIVTSSGGTLEWQDSVGRDNTGGIFR
                     RHDSYATIPTGLSILEAGQSIFCGMWIKKTASPTDWRSYNMSMFQAFLSGIRMSDGVL
                     LIQLSVGTTTTVISGLASANMNLNQWYYVIFEFRRASAAGVADGGFRVWKDGVLIGDK
                     CIYDNYGRTSLSTFCLGSYLTASTSGDYIFDDVRIGETLADVEPARPAVDGELPQSAR
                     MLVLFNRNSADSESFARSLVPDTNIVSLPCSSDETLTDYATFQAEIETPLKEFLAARP
                     LLASQITCIIAGYMVPGYFYSGNYLLTTCSRLMDIDNAFSANKTNPLYGTSGRLTATV
                     LQTAGVRLAARVDGTTMADCQLLKTIGDATVMIEEGKTVFMSGTPTLDVQLLRLPIKQ
                     LAAGDVISDDVLNFNIPPVSGISTVSADEPGSRLAIILNNATDITRGELAYATADWHY
                     RAMIFSSGRYAFDFSAFCGMLRDGGTIAEAFAVACEKIDAKYQLGAWPLLRAVFPKAG
                     YNVYLGNGAQSDIDYSQIYAALPISTTDFEIKIPNQPDFTRVMCVRKTSEYGMEETRG
                     RIVMLESDSDGLINLQLPIPYDLTTRETGENNVELSFSCFVDEGQEQPSLFEILGDNG
                     SGKIDIANPLAEIPFNNDEREYSVIITAATRPAIFAVRAKLENRKGKQTDAITVLPLR
                     IPVQPEVLI"
     gene            4332..5765
                     /locus_tag="PLK08_05125"
     CDS             4332..5765
                     /locus_tag="PLK08_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52917.1"
                     /translation="MNTTTIHPDDAFKTSYRVRPLRVYVEGIRRRDMEVRTWKMNDEN
                     DFGTISLAGIRDGADSAEFNLPAIGSSVKILPAEGESWEFSGSIVKHTVSVGDGEELP
                     IALVEHELIVTASQKITGRYIWDGDAAIRTNDALRFNYDAADMASISAIEFNGRTGQV
                     FAADGADSILWSVGGALAYLMAAELPKIFESQSYNELMSLAGDITLCKYDATKKTLLQ
                     AFEEIAAYGGLNLRVSRRGMGLVFYRAGQGSRCTTLKLQLPGSELSTSASNLHSGKIT
                     MTARPANRPVRAMGAYKRYEASFKLKPGWDTSLETTLWRHYNRTTSDDWNKYRFVYRK
                     WILNESSQSSDVEPFDFSTIDAESFKSNRKRKFLPCLSGEKNRSYGYYVEYRESEYYA
                     WKPWPGSVWISGSECSIIFDDVFLPADFLSTAYSHSAEVRITASVDSDVRLVAEVSGD
                     SRHSWDIHDYSGSATAWMVTSTRFLQR"
     gene            5821..6030
                     /locus_tag="PLK08_05130"
     CDS             5821..6030
                     /locus_tag="PLK08_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52918.1"
                     /translation="MRYAEYNSRGCRMEVSLGWIDATVFVGDVVTQIDGRDIELMSHT
                     LGQPAVKSVRHEFGDEQKTHLILEG"
BASE COUNT         1671 a         1453 c         1474 g         1471 t
ORIGIN      
        1 attacattct ggcaggtgcc gctggtgatt acttcaaggc gactcttgac ggattggagt
       61 tattctcaag cgggccaggt gaattgatat gcgaaagctg gcctcgtgaa actatcagac
      121 gcggtttctc cggcctgtgc ggcgaaattc tgctggatat gggtcggcga agccggcgta
      181 taacccaacg cggctttctg agagcacaga ccgttgaaca actttacctg caaacagcag
      241 ctatcgaatc catggccgac ggcatggctc accttttaat agatgatgac ggagtaacct
      301 atcaaaaggt cattatggaa aaatttgaaa aaccagatgc cattaataaa tcagacatct
      361 gttcatgcga atatgaaata atctatcggc aattaccatg agtgaaatac agcatattca
      421 ggtcgacaaa ctttggattg tggaaacatc gacgccgtcg gtattggaag tcagattcat
      481 cgcaccggca ggagcagaag aagactggca ctatcagctc tacctgaatg gaacgtgggc
      541 ggatacgtct gaaagttcca accagcgagc cttttcggta catgcgcaga aatatccgca
      601 gatgtttgct gtttttgccg tggaaccggc aggccgaaac gtcaaccttt ccgcattatt
      661 gcccgaagat ttacgcaccc cgtcgtgggt ttgcaatttc cgaatgaccc aggataccgc
      721 gttcgaacct ggcgacagga tggaaattta tcatgacgac ggcagtggca caatggacga
      781 acagccagcg cttcgatggc tagcctggcc gccggcaata tctcacacgg gttggggcaa
      841 tgcaacactg cttaacagcg gctttggcat agacggcagt aatgctccag gtatgtctgc
      901 accttttggc ataggaccaa tggggctcgg cagttttgaa ttaaagactg ttctgcctct
      961 taaaaacagt ggccatcata atatcagagt tctctgccgc agcagcgacg gcatcgcgtc
     1021 caagtcaaaa gcattgacat ttttctcatc ggctccgccg tcacggcctg gcaagataac
     1081 ttttatcagc cacaatccat ccaccacaat tgcaaaattc agaatcagat aaaaagaggt
     1141 aatcatgagc gaactatatc catcagatgc aataatcaat gctctgtccg gcacaaaaga
     1201 ttcggggcag gaagtacaat atccggcaat ctatgaaacg ccgtattaca ctacgtttta
     1261 caagatgatt tatcgccttt tgaacgtagc ccggcgtgcc ggagatttcc gtatttataa
     1321 agatggtgat ttgactttcg gcgttcgtgc cggcaaggtc agcctgggca attcaaggtt
     1381 tgatttcagc ggagccgctc agcaagtgct gaccgacaat gccacaaaca gcatatatct
     1441 gacggttgac ggcgtcgtac acatatccac gtctggtttt ccatcggtgt cgactacggc
     1501 atttatcccg ctggcaatca tttcaacagc caacggcagc tacgattttg aagatattac
     1561 agatcttcgt gctttatcca tgtttcagtt caacacgaat cttacgccag acaacgcaca
     1621 gatattgacg acatccaaag tggctgactg tttgcatact cacgattccg caggaatagc
     1681 caatgaagct ctcggcggag tgcacctcaa ctcccagctt cgtaaaagtt gcagttatgc
     1741 cgtacttaca accacagaca atgcaaacgg tacagccacc ataactatat ctatacgata
     1801 tgcgtcaggc acatcgtcta caaatctcaa ccttgtcaga atctggactt ctgacagcag
     1861 cctgacaata cccagtgaac aggcgacatt aacaatacaa cagggtctgg tcatgaatac
     1921 ctatcaggca aatgtggacc ttgaaatact gaccgcacaa acaggcaaca tagtaatgct
     1981 cgcatcatcg tcagccaaca aaagttttca tgtccatgtt gaatttcgcg gcattgttga
     2041 cgtttattcc gtttccataa ctggcaacta aaatggaaat ttcatttaca gccaatgacg
     2101 ttatctatgt cgctcattac gtcggcgctc gtgacggcaa ggccggccgt tggtggcaat
     2161 gcgacgaact ggacttatgg ctacaaccac ccgtaaatac agaggcgaat gagttgccga
     2221 cactttttgc ggaagcaata ttacagcagg agatttcaga tggctttgct gctgcactat
     2281 gaatttgaca cagcggacag cctggacggc tggactattg ttacatcaag cggcggaacg
     2341 cttgaatggc aggattctgt cggccgtgat aataccggag gaatcttcag acgacacgat
     2401 tcctatgcga ccattccaac gggattatcg atattagagg ctggccaaag cattttttgc
     2461 gggatgtgga taaaaaagac agcttctccc actgattggc gaagttataa catgtctatg
     2521 ttccaggcat ttctgtcagg catacgaatg tcagacggcg ttttgctcat ccaattatct
     2581 gtcggcacta caacaactgt catttccggc cttgcttcgg caaacatgaa tctgaatcaa
     2641 tggtattatg taatttttga attcaggcga gcaagcgcag cgggcgttgc tgacggcggt
     2701 tttcgagtct ggaaagacgg cgtcctcatt ggcgataaat gcatttatga taattacggg
     2761 agaacttctc ttagcacatt ctgcctcggt tcatatttaa cggcttcaac cagtggggat
     2821 tatatattcg atgatgtgcg gataggcgaa actctggccg acgttgagcc ggcacggcca
     2881 gccgtcgacg gcgaacttcc gcaatcagcc cgcatgcttg tactgttcaa ccgtaacagc
     2941 gcagacagcg aatcgttcgc ccgcagcctc gtacctgata cgaatatcgt gagtctgccg
     3001 tgcagcagcg atgaaacatt gaccgactac gccacgtttc aggctgaaat tgaaactccg
     3061 ctaaaagaat ttctggcggc tcggccattg ttggcatcgc agattacatg cataatcgcc
     3121 ggctatatgg tgcctggata tttttatagc ggcaactatc ttcttaccac atgttcacgg
     3181 ctgatggata ttgacaatgc tttttcagcc aacaaaacaa atccgctgta tggcacatcc
     3241 ggccggctga ctgccacagt gttacagacg gccggcgtga ggcttgcggc acgcgttgac
     3301 ggaacaacaa tggctgactg ccagctgtta aaaactatcg gcgacgcaac tgtcatgata
     3361 gaagaaggta aaaccgtttt catgtccggc acgccaacgc tggatgtgca actcctgcga
     3421 ctgccaatca agcagttagc cgccggcgat gttataagcg atgatgtcct taattttaac
     3481 attccgccgg taagcggcat ttcaaccgtc agtgccgacg agcctggctc aaggcttgca
     3541 ataatcctga acaatgccac cgacataact cgcggcgaac ttgcctatgc gacggctgat
     3601 tggcattaca gggcgatgat tttttcatcc ggcagatatg catttgattt ttctgcattc
     3661 tgcggcatgc tgcgcgatgg cggtacaatc gcagaagcat tcgccgtcgc gtgtgaaaaa
     3721 attgacgcaa aatatcagct tggcgcgtgg ccattgctga gagcagtctt cccgaaagcc
     3781 ggatataacg tttacctcgg caatggggca caaagcgata tcgactattc acaaatctac
     3841 gctgccttgc cgatatcgac aacggatttt gaaatcaaaa tcccgaatca gcccgatttc
     3901 actcgcgtaa tgtgcgtaag gaaaacttca gaatacggaa tggaagaaac gcgcggccga
     3961 atagttatgc tggaatctga ttccgacgga ctaataaatc tgcaactgcc aatcccatat
     4021 gatctgacca cccgcgagac gggagaaaac aatgtggagc tttccttcag ctgttttgta
     4081 gacgaaggcc aggaacagcc gtcgttattt gaaattctag gcgataatgg aagcggcaaa
     4141 attgatatcg ccaaccctct ggctgaaata ccgttcaata atgatgagag ggaatattcc
     4201 gtcatcataa ctgctgcaac tcgtccagct atttttgccg ttcgtgccaa attggaaaat
     4261 cgcaaaggca aacaaacaga cgccattacg gttctgcccc tgcgaatacc agtccaaccg
     4321 gaagtgctga tatgaatacg actactatac atccagatga tgcattcaaa acatcataca
     4381 gggtcaggcc attacgtgtt tacgtcgaag gcatacgaag acgggacatg gaagttcgta
     4441 cctggaaaat gaacgatgaa aatgatttcg gcaccatatc tcttgcagga atcagggatg
     4501 gggctgactc cgcagaattc aatctgccgg cgataggctc atccgtaaaa attttgcctg
     4561 ccgagggcga aagctgggaa ttttcaggca gcattgtaaa gcataccgtc agcgtaggcg
     4621 atggagaaga attgcccata gccctggtgg agcacgaact gatagtgacg gcgagccaga
     4681 aaatcaccgg cagatacatt tgggatggag atgccgcaat cagaacgaat gacgctctgc
     4741 gttttaacta tgatgccgcc gacatggcca gcatatcagc gattgaattt aatggccgta
     4801 ccggtcaggt ttttgcggcc gatggcgccg acagtatttt atggtcggtc ggcggcgcgc
     4861 tggcatatct gatggctgct gaactgccga aaatattcga aagccaatct tacaatgaac
     4921 tgatgtcgct ggcaggagat attactctct gtaaatatga tgccacaaaa aaaacgctac
     4981 tgcaggcatt cgaagaaata gcagcttacg gcggattgaa tctgcgagta agcaggcgag
     5041 gcatgggatt ggttttttac agagccggac aaggatctag atgtacgaca ttaaaactgc
     5101 aattaccagg cagtgaactt tcaacgagtg caagcaatct gcactccggg aaaataacca
     5161 tgacggccag gcctgcgaat cggcccgtac gagctatggg ggcatataaa agatatgagg
     5221 ccagttttaa actgaaacca ggatgggaca cgtcgcttga aaccacttta tggcgacatt
     5281 acaaccgcac tacttccgat gattggaata aatacagatt cgtttacaga aaatggatat
     5341 tgaacgaatc atcgcaatcg tccgatgttg agccatttga tttttcaacc atagatgccg
     5401 aaagtttcaa atcgaaccgt aaaaggaaat ttctgccgtg cctctccggc gaaaaaaacc
     5461 gcagttacgg atattacgtc gaataccgag aatccgaata ctacgcatgg aaaccgtggc
     5521 ctggttcagt ctggataagc ggcagcgaat gttcgattat ttttgacgat gtatttttgc
     5581 ctgccgattt tctcagcacc gcatattcac actccgcaga agtgcgaatc acggcttcgg
     5641 ttgactcaga cgttcgactt gtcgccgagg tttcaggcga ttccagacat tcatgggata
     5701 ttcacgatta ttcaggctct gcgacagcat ggatggtcac aagtacgcgt tttttacaaa
     5761 ggtgacggtg taaatcatca ggttttacga gacgattcgt ccaggctgct cagcattgcc
     5821 atgcgttatg ccgaatacaa ttcgcgcggc tgccgcatgg aggtgtcgct cggatggatt
     5881 gacgccaccg ttttcgtcgg cgacgtagta acgcaaatag acggccgcga tattgagctg
     5941 atgtcacata cgcttggcca gccagccgtc aaatctgtta gacatgaatt cggcgatgag
     6001 cagaaaactc atctgatact ggaaggataa atccatgtcc ataaatgtaa ataatccata
     6061 ctcgcaact
//
LOCUS       DAONFI010000122         6056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_15319_length_6056_cov_4.208799, whole genome shotgun sequence.
ACCESSION   DAONFI010000122 DAONFI010000000
VERSION     DAONFI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15319_length_6056_cov_4.208799"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="lpxD"
                     /locus_tag="PLK08_05135"
     CDS             <1..849
                     /gene="lpxD"
                     /locus_tag="PLK08_05135"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPS52919.1"
                     /translation="PADLPAVAGKTLIRVDDVEAAIAALLAVIAPGEDLPPVGIHGSA
                     VVAQDAELAPDVAVGPCVVIGGNARIGRGTVLCAGVKIGRDAVVGENCVLAEGAVLRY
                     GCVLGNRVRIGCNSVIGQDGYGYFFRNGVHNKIIHAGNVVVEDDVEIGACSCVDRAKW
                     GSTRIGAGTKIDNLVQVAHNVQIGRGSLLVAQTGIAGSAKIGNYVVLGGGAGVRDNIT
                     VGDGVQCAAHSAIAQSVDAGKTVAGTPAKDAREIFRELLALPKLPDLLKQVRKMEKQI
                     QDFLGH"
     gene            869..2173
                     /gene="lpxC"
                     /locus_tag="PLK08_05140"
     CDS             869..2173
                     /gene="lpxC"
                     /locus_tag="PLK08_05140"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPS52920.1"
                     /translation="MKMQKTIKTHAVVEGRGLFNGTPGRVRFCPAPEDTGIVFVRTDT
                     VSPVEIPADIDYVVDRLHRTTSLAVGEVSVETVEHVLSAIWGLDIDNLRIEVDNPEIP
                     NVDGSSLPYAQALKQAGLEEQDMPKDVHLLDEPIVVSEGDMMLTALPGSIDSLDLLYD
                     MEYPVASIGRQLCSFRLGKDDYIGQLASARTFLLENEAKQFQAMGLGRHLTARDILVM
                     NDAGPVDNELRYPDEHARHKMVDLIGDLALLGAKFCGRVVAYRSGHEMNHSLVRKLRE
                     AIEVKTQAGSGEPVMDIRRIMRLLPHRYPFLMVDRIVSIDGDKKAVGLKNVTINEPFF
                     QGHYPNLPIMPGVLIVEALAQMSGILLGRRLEHTGKVAVLLSMDKVKMRRPVRPGDQL
                     ILEAEALHARSRTGHCRCRALVGDEIACEAEIKFMLVDSEPM"
     gene            2353..3123
                     /locus_tag="PLK08_05145"
     CDS             2353..3123
                     /locus_tag="PLK08_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52921.1"
                     /translation="MAKISKLSVVEKGARLADDVVVGPFCYVGRKVEIASGCVLENNV
                     TIVGRTAIGEDTRIFPMSMIGMTEDGDEDGVCVIGKANAVREHVTILAGTTERPTELG
                     TGNLIMVGCNISGSVRVGNHVILANCTNLGSRSVVEDYVRMSAFSYLCEDIIVGSYAF
                     VAGLIIIDRNIPDYSMVQGVPPRVRGVNTHNLKACGFGEGDIRELKRVFRDLFNGGDH
                     LNEKVLAELDAQENLNVYVRKVVDTIKQQRQAGLKGAW"
     gene            3123..4007
                     /gene="lpxA"
                     /locus_tag="PLK08_05150"
     CDS             3123..4007
                     /gene="lpxA"
                     /locus_tag="PLK08_05150"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPS52922.1"
                     /translation="MAVKVEIIREVHPSARIASDVKIGSFSVIGPDVTLGPGTVIGRN
                     VTIAGNTTIGSGNFIDDGSVLGVVPQDLKYRGSPALLIIGHNNRIGRRVTMHIGTEPG
                     GFVTRVGDNNVFREGSHMAHDCFVDDGAVIGRSVQLAGHILVQNGAVLEDMVGVHHFV
                     TIGRYARISSMTPVRRDVPPYTVFGGGNEEAPSVRGVNEDGIKTAGLAEDETADLRRA
                     LGELFEDEAALQTKIEQLVNMGVEGEVAYLCEFCQQSLQGKFGRYREVYRGLIPPEAA
                     AVLPPEKLSELERILKIK"
     gene            4053..5102
                     /locus_tag="PLK08_05155"
     CDS             4053..5102
                     /locus_tag="PLK08_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS52923.1"
                     /translation="MAMRFAVIGCGRMGKHHARTLQNLESADLVAVVDANEDAAKSLA
                     AQRNCKAFTSATDVMDDIDAAVIATPTVSHLKAAWPLVEAGKHLIIEKPYCDDVAAGG
                     ELIKLAGKSGSKIIIGHSERFNPACIAMSKYDIRPKFIEAHRISPFTFRSADIGVVLD
                     MMIHDIDLVLMMARSRVVDVQAIGVSVIGKHEDICNARLRFASGCVANITASRLAIKT
                     ERKMRIFSEEHYLSIDYAKKCGLVIEKNQNLDIIQMARDMDVDDIAELAQSVDYTKLL
                     KFEELVPDESKDPLTRQAEAFIDMVTNGAETLACKVEEGLAAVVTANAIISAISQHKW
                     DGDAGSRVGSDIISR"
     gene            complement(5153..5944)
                     /locus_tag="PLK08_05160"
     CDS             complement(5153..5944)
                     /locus_tag="PLK08_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPS52924.1"
                     /translation="MKNLVGGSMERLRAKCRREGTTLDDLLKMQRRELTVQIYLQSKF
                     MPAIVITRRMLWNFYRDHQDLYHTDKKVQMQIIAAPFDQFLPENVTQPTSEEKQIASL
                     AARRHIENAYRMLLSGSDFDKIAMEMSKGPMAAKNGIWPAMTKGNFREAAVEQAAFEL
                     PTGRFSSIIEGDNGYYIVRARKVYPGKKISFEQAQEGVENSADPSRQIAPIEEILRRD
                     QFAKLRTEYMANVYKQATIVIPDNFYYETLRQAMLRYRPGAKLQN"
BASE COUNT         1380 a         1511 c         1823 g         1342 t
ORIGIN      
        1 ccggctgatt tgccggcagt tgccggcaag acgctgattc gcgtcgacga tgttgaagct
       61 gctattgccg cactgttggc tgtgattgcc ccgggtgaag atttgccgcc agtcggcata
      121 catggctcgg cggtagttgc acaggatgcg gagcttgccc ccgacgttgc agtcggcccg
      181 tgcgtcgtca tcggcggaaa tgccaggata ggccgtggta cagtcttgtg tgccggcgtg
      241 aaaatcggca gagatgccgt cgtcggcgaa aattgcgtat tggcggaagg cgcggttcta
      301 cgctacggat gcgtattggg caatcgtgtg agaatcggct gcaacagcgt catcggccag
      361 gatggttacg gatatttttt ccgtaacggc gtgcacaaca agattattca cgcaggcaac
      421 gtcgttgtgg aagacgacgt tgaaatcggc gcgtgcagct gcgtggacag ggctaaatgg
      481 ggcagtacca ggataggcgc aggaacgaag atagacaatc tcgtgcaggt ggctcacaac
      541 gtgcagatag gccgcggttc tctgctggtg gcgcagaccg gaattgccgg cagtgcgaaa
      601 atcggcaact acgtggttct gggcggcggt gctggcgttc gcgataatat aaccgtcggc
      661 gacggcgtac agtgcgcagc ccatagtgca atcgcgcaga gtgttgacgc aggcaagacc
      721 gtagccggca cgccggctaa agatgccagg gagattttta gagaattgct tgcacttccg
      781 aaattgcccg accttttaaa acaggtcagg aaaatggaaa agcagattca ggattttctg
      841 gggcactgac cccaaagtga ggttgctcat gaaaatgcag aagacaatca agacgcacgc
      901 cgtggttgaa ggcagaggct tgttcaacgg cacgcctgga cgggtgcggt tctgccctgc
      961 gccggaggat accggcatag tgttcgtccg cacggataca gtttcgccgg ttgaaattcc
     1021 ggcggatata gactatgtgg tcgacaggct gcatcgcact acgagcctgg ctgtcggcga
     1081 agtcagcgtc gagacggtcg aacatgtgct ctcggcgata tggggactgg atatagacaa
     1141 ccttcgcata gaagtggaca atcctgaaat tccgaacgtg gacggctcat cgttgcctta
     1201 cgctcaggcg ctcaagcagg ctggcctgga agagcaggat atgcccaaag acgtacacct
     1261 cctcgatgag ccgatagtcg tctcggaagg cgatatgatg ctcacggcgt tgcccggctc
     1321 gattgacagc cttgacctgc tttacgacat ggaatacccc gtcgccagca taggccgtca
     1381 gctttgcagc tttcggctgg gtaaggacga ttacatcggc cagcttgcct cggcgaggac
     1441 ttttctgctg gaaaacgaag ccaagcagtt tcaggccatg ggccttgggc ggcatcttac
     1501 ggctcgcgac atacttgtga tgaacgacgc cggcccggtc gataatgagt tgcgttatcc
     1561 ggacgaacac gcgcgtcaca agatggtcga tttgataggc gaccttgcat tgctcggcgc
     1621 gaaattctgc ggccgtgttg ttgcgtatcg cagcggccat gagatgaacc attcgctcgt
     1681 tcgcaaactg cgagaggcga tagaagtaaa aacccaagcc ggcagcggcg aacccgtcat
     1741 ggatatccgc cgcataatgc gactgctgcc gcatcgctac ccgttcctga tggttgaccg
     1801 catagtgtca atagatggag acaaaaaagc ggtaggcctt aaaaacgtga ctataaacga
     1861 accgttcttc cagggccatt atccgaatct gccgataatg cccggcgtgc tcatagtcga
     1921 ggcgctggcg cagatgtccg gaatattgct gggaaggcgg ctggaacata ccggcaaggt
     1981 agccgtgctg ctgagcatgg acaaggtcaa gatgcgacgg ccggttcgtc ctggcgacca
     2041 gcttatattg gaggcggagg ctcttcacgc ccgtagccgt accggccact gccgatgccg
     2101 ggcactcgtc ggcgatgaaa ttgcctgcga ggcggaaata aaattcatgc tcgtagattc
     2161 agagccgatg tgattttttg taacgaaaga caatgttaaa aaagcgagtt ttgcgggtta
     2221 aaaaccgtaa tatcgtattt atcgggcttt cgcctggccg cctgagggcg gatgctttaa
     2281 tctgcggcgt tttttgaaaa aaaccgtgga actacaaagg acatattgaa aaatccgaaa
     2341 gataaaaccg agatggcaaa gatatcgaaa ttaagtgttg tggaaaaagg ggccaggctg
     2401 gctgacgatg tggtcgtcgg gcctttctgt tacgtcggca gaaaagttga gatagcgtca
     2461 ggctgtgtgt tggagaacaa tgtgactatc gtcggcagga cggctatagg cgaagacacc
     2521 cgcatatttc ccatgtcgat gataggcatg actgaagacg gcgatgaaga cggggtgtgc
     2581 gtcatcggca aggctaacgc cgtccgcgag catgtcacga tattagccgg cacgactgaa
     2641 cggccgacgg aacttggcac gggcaatctc atcatggtcg gctgcaatat ttccggcagc
     2701 gttcgggtgg gcaatcacgt gatattggct aattgcacga atctcggctc gcgcagcgtg
     2761 gttgaggatt acgttcggat gagcgcgttc agctatcttt gcgaagatat catagtcggt
     2821 tcatatgcat ttgtagccgg ccttatcatt atcgacagga acattcccga ttattccatg
     2881 gtgcagggcg tgccgccgcg ggtgcgtgga gtcaacacgc acaatctcaa ggcgtgcggc
     2941 ttcggcgaag gtgacatacg tgaactcaag cgggtctttc gcgacctgtt caacggcggc
     3001 gatcatctga acgagaaagt tttggcggaa ctcgacgccc aggagaatct caacgtctac
     3061 gtccggaaag ttgtcgatac cataaagcag cagcgtcagg ctggcctgaa aggggcctgg
     3121 tgatggctgt gaaggtggag ataatccgcg aggttcatcc ctcggctcgc atagcatcgg
     3181 acgtcaagat aggttctttt agcgtaatag ggcctgacgt cacgctgggg ccaggcacgg
     3241 ttataggccg gaatgtgacg atagccggca atacgacgat tgggtcgggc aatttcatcg
     3301 acgacggcag tgtgctgggc gtcgtgccgc aggatttgaa atatcgcggc tcgccggcgc
     3361 tgttgataat tggccacaat aaccgaatcg gccgtcgtgt gacgatgcac atcggcactg
     3421 agcctggcgg attcgttacc cgcgttggcg acaacaatgt ctttcgcgaa ggttcgcaca
     3481 tggctcacga ctgttttgtg gatgatggcg ccgtaatcgg ccgcagcgtc cagctggccg
     3541 gccatatttt agtgcagaac ggagcggtac tggaagacat ggtcggcgtg catcattttg
     3601 tgacgatagg ccgttatgcg cggatatcat ccatgacgcc ggtacgtcgc gatgtgccgc
     3661 cgtacaccgt attcggcggc ggcaacgaag aagcgccgtc tgtcagaggc gtgaatgagg
     3721 acggcataaa aacggcaggc ctggctgaag acgaaacggc agacctgcgt cgcgcgttgg
     3781 gcgaactttt cgaagacgaa gccgccctgc agacgaaaat agaacagctc gtaaatatgg
     3841 gcgtcgaagg cgaagttgcg tatctctgcg aattctgtca gcagagcctg caggggaaat
     3901 tcggccgata ccgcgaagtc tatcgcggcc tgattccgcc ggaggctgcg gctgtcctgc
     3961 cgccggaaaa attatccgaa cttgaacgaa ttctgaagat aaaatgattg caattttttg
     4021 cgctatcgtg aaatttttat tcgaggttta aaatggcaat gcgttttgcg gtaatcggct
     4081 gcggcaggat gggaaagcat catgcccgta ctttgcagaa tctggagtcg gcggacctgg
     4141 tagccgtcgt cgacgccaac gaagatgccg ccaaatctct ggctgcgcag cggaactgca
     4201 aagcctttac gtcggcaact gacgtcatgg acgatattga tgctgccgtc atcgccacgc
     4261 ctaccgtgtc gcatcttaag gctgcgtggc cgctggtgga ggctggcaaa cacctgataa
     4321 tcgaaaaacc gtactgcgac gacgttgccg ccggcggcga actcataaaa ctcgccggca
     4381 aaagcgggtc gaaaatcatc atcggccatt ccgagcggtt caatccggcg tgtatcgcca
     4441 tgagcaaata cgacattcgc ccgaaattca tcgaggctca ccgcatcagc ccttttacct
     4501 tccgctccgc cgatatcggc gtggtgctgg atatgatgat tcacgatata gaccttgtat
     4561 tgatgatggc tcgcagcagg gtggtcgatg tgcaggctat aggcgtgagc gtcattggaa
     4621 agcacgagga tatctgtaat gcgaggttga ggtttgcgag cgggtgcgtg gctaatatca
     4681 cggctagccg gcttgctata aaaaccgaac gcaaaatgcg gattttttcc gaagagcatt
     4741 atctgtccat cgactacgcc aaaaaatgcg gcctcgttat cgaaaaaaat cagaacctgg
     4801 atattatcca gatggctcgc gatatggacg tggacgacat cgcagaactc gcacaaagcg
     4861 tcgattacac caagctgctg aaattcgagg agctcgtccc cgacgaatcc aaagatcccc
     4921 tgacgcggca ggcagaggcg tttattgata tggtgacgaa tggtgcggaa acgctggcct
     4981 gcaaggtcga agaaggtctg gcagcggtgg tgacggctaa tgcgataatc agtgcgatat
     5041 cgcagcacaa gtgggacggc gatgccggca gccgcgtcgg ctcagacatc atctccagat
     5101 agaaaaacgc tgaatgccgc ctgcaaaccg gctctctgga gcataccgtg ctctaattct
     5161 gtagtttggc gccgggccgg taacgaagca tggcttgccg gagcgtctcg taataaaaat
     5221 tgtcgggtat gactatggtc gcctgtttat agacgttggc catatattcg gttcgcagtt
     5281 ttgcaaactg gtcgcggcgg aggatttctt ctatcggggc tatctgtctg gacgggtcag
     5341 ccgaattttc gactccttcc tgtgcctgtt caaatgaaat ctttttacca ggatagacct
     5401 ttctggcccg cacgatgtag tagccgttgt cgccttcgat gatgctgctg aagcggccgg
     5461 tcggcagttc aaacgccgcc tgttcgacgg cggcctcgcg gaaattgcct tttgtcatcg
     5521 ccggccatat gccgtttttc gccgccatcg gccctttgga catctccatt gctatcttgt
     5581 cgaaatccga accggagagc agcatgcggt atgcattttc tatgtgacgt ctggcggcta
     5641 ggctggctat ctgtttttct tcgctggtgg gttgagtcac attttccggc aggaactgat
     5701 caaacggtgc ggcgattatc tgcatctgga cttttttgtc cgtgtggtac aggtcctggt
     5761 ggtcgcggta gaaattccac aacattcgcc gcgttatcac gatagccggc atgaatttcg
     5821 attgcagata tatctggacg gtcaattcgc gtcgctgcat cttcagcagg tcgtccagcg
     5881 tagtgccttc acggcggcat ttcgcacgca gccgttccat actgccgccg acgaggtttt
     5941 tcatgtcccg aaccatccgg gaaaacggcg gcgagagccg cagcgtcaat ctggaggccg
     6001 agatccgccg catggccgag gtggtgacca aggtcgcccg gcaaatgggg atcgaa
//
LOCUS       DAONFI010000123         6012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_15472_length_6012_cov_4.114655, whole genome shotgun sequence.
ACCESSION   DAONFI010000123 DAONFI010000000
VERSION     DAONFI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15472_length_6012_cov_4.114655"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /gene="rpsM"
                     /locus_tag="PLK08_05165"
     CDS             complement(<1..205)
                     /gene="rpsM"
                     /locus_tag="PLK08_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPS52925.1"
                     /translation="MPRLAGVDIPNDKPVWISLRYIHGIGPKFARDICARMGIEDKVR
                     AKDLTEDQLAEMAAMLTGEYTIEG"
     gene            complement(236..349)
                     /gene="rpmJ"
                     /locus_tag="PLK08_05170"
     CDS             complement(236..349)
                     /gene="rpmJ"
                     /locus_tag="PLK08_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPS52926.1"
                     /translation="MKVRSSVKRICENCRVIRRKGVVRVICTNPKHKQRQG"
     gene            complement(453..1223)
                     /gene="map"
                     /locus_tag="PLK08_05175"
     CDS             complement(453..1223)
                     /gene="map"
                     /locus_tag="PLK08_05175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPS52927.1"
                     /translation="MAIKIKNPDQIEKMYAAGQIVRETLDRLGEIITPGITTKELDEE
                     AKNVCKQHDAVCLFYKFPGMGGPFPGNICASINEEVVHGIPSKSRIVSDGDIVSVDFG
                     VLKDGWCGDAARTFCVGNVEPENVRLVEVTRKSLELAIELMPISRTWSQVAYKMQEFI
                     RGEGFSVVEDFVGHGIGQSMHEDPQVPNFVTHSLKLRDIKFRPGMVLAVEPMVNMGVP
                     ACRILPDGWTVVTADRKPSAHWEHTIAIVENGIRVLTA"
     gene            complement(1281..1934)
                     /locus_tag="PLK08_05180"
     CDS             complement(1281..1934)
                     /locus_tag="PLK08_05180"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPS52928.1"
                     /translation="MRIVLIGPPGAGNGTQAQRLAKKYGMTHLSSGDIFRAEKASGSE
                     LGKKLAAIIDRGELVPDDVVVSIMAKAIKKAGDSLLLDGFPRTVAQAESLDETLAGMG
                     ERLDAVVVIKADDNAIIERITGRRYCPSTGKVYHIRFMPPKTEGLDDETGEKLVQRDD
                     DREAVVRERLEVYHRQTSPVIEYYRRSGVVKVVEIDGMGSPDEVTVRMTQAVESLKK"
     gene            complement(1931..3313)
                     /gene="secY"
                     /locus_tag="PLK08_05185"
     CDS             complement(1931..3313)
                     /gene="secY"
                     /locus_tag="PLK08_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPS52929.1"
                     /translation="MIENIFTIPELLGKLLFTIALLAIYRVGFAIPLPGINQDAIRSL
                     FQGKGGGLFGEAMEYYGMFTGGSLSQSSLFGLGIMPYITASIIFMLLAQVVPAIEKLQ
                     KEGPAGQKKIHEYTRYSTVALCIFQAIMFLKWISTQTLPGGLSMVYPSWQGSWLLFTT
                     WVICLTAGTMFLMWLGEQIDEYGIGNGISLLIMAGIVARLPYAIRELFAKSSFQLGDS
                     GDIGPMKIIVLALSFVFVVAGAILITQGQRRIPIQQAKLTRGRRVYGGQRHYIPLRVN
                     HGGVMPIIFASSLMMIVGLVGDWIGRLFGNGTGFWASFFQAIPESFRPGAFVYTILEV
                     LMIYFFAYFWTTVQFRPKDMSDQLRDMGHFIPGLRPGKRTADYLETVMERITYCGAAF
                     LAIIAVLPALVASKMGIPFQVSAFLGGTGLLILVSVVLDLIQRIEAQLVMRNYGGFLG
                     GSTRIKGAYA"
     gene            complement(3717..4163)
                     /gene="rplO"
                     /locus_tag="PLK08_05190"
     CDS             complement(3717..4163)
                     /gene="rplO"
                     /locus_tag="PLK08_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPS52930.1"
                     /translation="MKLDDILSAAGKYKKRKRIGRGVGSGKGKTSGRGHKGYGARAGS
                     SRRLGYEGGQNPTIARMPQRGFNNANFRMVYQVVNVAALDAAFDNGATVDAASLKKVS
                     LIGDAGKPVKILGNGDLSKKLTVVAAKFSASAKEKITNAGGSAEEK"
     gene            complement(4160..4657)
                     /gene="rpsE"
                     /locus_tag="PLK08_05195"
     CDS             complement(4160..4657)
                     /gene="rpsE"
                     /locus_tag="PLK08_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPS52931.1"
                     /translation="MQKNTNEFEKAGGLEENIIQIYRCAKVVKGGRRFSFAALVVVGD
                     RNGRVGMGYGKANEVPNAVEKGKAAAIKNMVTVPLRGSTIPHEVKAKSGASSVVLIPA
                     GEGTGVIAGKKLRPLFELAGIHNLLSKAYGSTSPKNLLRAGMQALKVLRSADCVSKLR
                     GVEIK"
     gene            complement(4679..5053)
                     /gene="rplR"
                     /locus_tag="PLK08_05200"
     CDS             complement(4679..5053)
                     /gene="rplR"
                     /locus_tag="PLK08_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPS52932.1"
                     /translation="MKPIDLKQERRERRKRRVRKKIQGTALRPRLSVFRSNHNIYVQL
                     IDDETGRTLAAASTLEKEIVGKIGGNSRKNKTAAAVVGEALAAKAVEKGIKKIVFDRN
                     GYPYHGRIHELAEAARKGGLEF"
     gene            complement(5158..5721)
                     /gene="rplF"
                     /locus_tag="PLK08_05205"
     CDS             complement(5158..5721)
                     /gene="rplF"
                     /locus_tag="PLK08_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPS52933.1"
                     /translation="MSRIGKKPIEIPGGVDVAVAGSTVTVKGKLGSDSFTFNPQVTVT
                     VAEGQVVVDRKNDEQACRALHGTARALVNNMITGVTKGFERKLEVYGVGYGVQLQGKN
                     FTINCGKSHPEVLALPAGIQVEVENAQARGDSEPAKFTVKGVNKQAVGEFAAKCRRTR
                     PPEPYKGKGVRYTGEKVRRKVGKAFGS"
     gene            complement(5746..>6012)
                     /gene="rpsH"
                     /locus_tag="PLK08_05210"
     CDS             complement(5746..>6012)
                     /gene="rpsH"
                     /locus_tag="PLK08_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPS52934.1"
                     /translation="YVEDYTKIEDNKQGLLRIYLKYGPTGEDVVTELKRISRPGCRVY
                     RSAGELPRPINGMGISVVSTSFGVLSDRECREKNVGGELLCTVC"
BASE COUNT         1344 a         1758 c         1394 g         1516 t
ORIGIN      
        1 taccctctat cgtatattcg ccggtcaaca tcgccgccat ctcagccagc tggtcttctg
       61 taagatcctt ggcgcgaacc ttgtcttcaa tgcccatacg cgcgcatatg tcgcgggcga
      121 atttggggcc tatcccgtgg atataccgca aggatatcca tacgggcttg tcgttcggaa
      181 tgtctacacc tgccaaacgt ggcataagta tctcctaatg cttgattcct gaatatcagc
      241 cttgccgctg cttatgcttg ggattcgtgc agattacccg cactacgccc ttgcgtctta
      301 tgacgcggca attttcacat atccgtttta ccgatgaacg aactttcatg tcagcacctg
      361 catatcatgt caaaagttct gatttacgtt cctgatttct cgggctgcgc cgccaactca
      421 acatcggctg cgaaaccgaa aaccgctgcc gcctatgcgg tcagcactct tattccattc
      481 tcgacgatag ctatcgtatg ctcccagtga gcactgggct tgcggtcggc ggtgacgact
      541 gtccagccgt cgggcaatat ccggcacgcc ggcacaccca tgttcaccat cggctcgaca
      601 gccaatacca tgccggggcg gaattttatg tcccgcaatt taaggctgtg ggtcacgaag
      661 ttcggcactt gcggatcttc gtgcatcgac tgtcctatgc cgtggccgac gaaatcctct
      721 accacggaaa agccttcgcc gcgaatgaac tcctgcatct tatacgccac ctgactccag
      781 gttcgggaaa tcggcatcaa ttctatcgcc agttcaaggc tttttcgcgt aacttccacc
      841 agccgtacgt tttccggctc aacattgccg acgcaaaacg ttctagccgc gtcgccgcac
      901 cagccgtctt taagtacgcc gaaatctacg ctcactatat cgccatcact tacgatgcga
      961 gacttcgacg gtatgccatg gacgacctct tcgttaatgg aagcgcagat attgcccgga
     1021 aacggcccac ccatgcctgg aaatttataa aaaaggcaga cggcgtcatg ctgtttgcaa
     1081 acgttctttg cttcttcgtc aagttctttg gttgtaattc ccggcgttat tatttcgccg
     1141 agcctgtcca gagtttcgcg gacaatctgg ccggccgcgt acattttttc tatctggtca
     1201 ggatttttta tctttatcgc catttaccaa actttgggtt ttttatgcaa attttatgca
     1261 aaaaccctta aaaggagtct tcacttcttc aaggactcca cggcttgagt catccggacc
     1321 gttacttcat ccggcgagcc catgccgtct atttccacca ccttgaccac accggaccgg
     1381 cgataatatt ctataaccgg tgaagtctgt ctgtggtaga cttccagtct ttcacgaaca
     1441 acggcttccc tatcatcgtc acgttgtacg agcttttcgc ccgtctcatc atccagccct
     1501 tcggtcttag ggggcataaa ccttatatga tacacttttc cggtcgaagg acaatacctt
     1561 cttccggtaa ttctttcaat aatcgcatta tcgtccgctt ttatgacaac aacggcatca
     1621 agacgttcgc ccattccggc gagagtttca tccagcgatt cagcctgtgc gacggtacgc
     1681 ggaaaaccgt ccagaagcaa cgaatcgccg gcttttttaa tcgccttcgc cattatgctc
     1741 acgacgacat cgtccggcac aagttcgccg cgatcaatta tcgccgcaag ttttttgccc
     1801 agctcgctgc cgctggcctt ctcggcacgg aagatatcgc cgctcgaaag atgcgtcatt
     1861 ccgtattttt tcgccagccg ctgagcctgc gtgccattgc ccgcgcctgg agggccaatc
     1921 agcactatcc tcatgcatat gctcccttta tgcgggtact cccgccgagg aagccgccgt
     1981 aatttcgcat aaccagttga gcttcaattc tctgaatcag gtccaacacg acagacacaa
     2041 gaatcagaag gcctgtcccg ccgaggaacg ccgatacctg gaacggtatg cccatcttac
     2101 ttgccacaag tgccggcaaa acggcgatta tcgccaaaaa tgctgcaccg cagtaggtta
     2161 ttcgctccat aacggtttca agataatcgg ctgtacgctt gccaggccgc aggcctggaa
     2221 taaaatggcc catgtcccgc aactggtcgg acatatcttt gggccggaac tgaaccgttg
     2281 tccagaaata cgcaaagaaa taaatcatca gcacttcaag tatggtgtag acgaacgcac
     2341 caggcctgaa tgattcagga atcgcctgga aaaacgatgc ccagaatcct gtgccgttac
     2401 caaacagtcg gccaatccag tcgcctacca gaccgactat catcatcagc gacgaggcaa
     2461 agataatcgg cataacgccg ccgtggttta cgcgaagcgg tatgtaatgc cgctgtccgc
     2521 cgtaaacgcg acggcccctg gtcagcttag cctgctgaat cggaatacgt cgttggcctt
     2581 gcgttatcag aatcgcgccg gcaactacaa acacgaacga cagggcaagg acgataatct
     2641 tcatcggccc tatatcgccg ctatcgccga gctggaaact gcttttggca aaaagttcac
     2701 gaattgcata cggcagacgt gcaacgatgc cagccatgat aagcaggctt atgccgttgc
     2761 cgatgccgta ttcgtcaatc tgttcgccca gccacatcag gaacatcgtg ccggcggtca
     2821 gacagataac ccacgtcgta aacagcagcc acgaaccctg ccacgacgga tacaccatcg
     2881 aaaggccgcc cggcagggtc tgggttgaaa tccacttcaa aaacattatc gcctgaaaaa
     2941 tacacaacgc caccgtagag tagcgtgtat attcatgtat ctttttctgg ccggcagggc
     3001 cttccttctg aagtttttct atagcaggca caacctgcgc cagaagcatg aatatgatgg
     3061 aagctgtaat atacggcatg attccaaggc cgaacagcga gctttgactc aggctgccgc
     3121 ccgtgaacat gccgtaatat tccatcgcct caccaaaaag tccgccgcct ttgccctgaa
     3181 aaagacttct aatggcatcc tggtttatgc ctggaagcgg tattgcaaaa cctactctat
     3241 aaatcgcaag cagcgcgatt gtaaaaagca gtttgcccaa caattccggt atggtaaata
     3301 tgttctcaat cacctgccgg aagttgtttt tgttgacgaa cacataccag tacgccgtaa
     3361 aaataatcaa agcgaaaact atttcgccga cagccggcat ggtttcctgc actcgccagt
     3421 tcagcgaggc cgcattagcc aagccgatgc cgcccttgac tatcatatag gcgcccgcac
     3481 cgccggctgc acccagagcc gccatcctga aagtttcata tgaagttttc gttttgaatg
     3541 caaacaatac gcaacaggcc agcacaatca ggccgcccgc tagtttagcc cattcaaact
     3601 ggtcgccgga gatatagtgt cctccgcaca tccatacgcc tgcggcgatg aacatcagga
     3661 ctgacaatat ttttatgata cgaaatccca cgtgcgatac ctcttgctgt caggatttat
     3721 ttttcctctg cgcttccgcc ggcattggtt attttctctt tcgcggatgc cgaaaatttc
     3781 gccgccacaa cggtaagttt cttgctaaga tcgccgttgc cgaggatttt gaccggtttg
     3841 ccggcatcgc cgataaggct gacctttttc agcgaggcag cgtcaacagt cgcaccattg
     3901 tcgaacgcgg catccagcgc ggcgacattt acaacctgat agaccatgcg gaagttggcg
     3961 ttattgaacc cgcgttgcgg catacgcgct atggtcgggt tctggccgcc ttcatagccc
     4021 aggcgacgcg atgagccggc tcgtgcgccg tagcccttgt gcccgcggcc ggatgttttg
     4081 cctttgcccg agcctacgcc gcggcctatg cgtttacgtt ttttgtactt gcctgcggct
     4141 gaaagtatat cgtctaattt cattttattt caactcctct gagcttgctg acgcaatctg
     4201 ccgaacgaag cactttcagt gcctgcattc cggcacgcag caggttcttt gggctggtcg
     4261 agccgtatgc cttgctcagc aggttatgaa tacccgccaa ctcgaacaac ggacgaagtt
     4321 ttttgcctgc aataacgcca gtaccttcgc ccgccggtat gagcaccacg ctggaagctc
     4381 cggatttagc cttgacttcg tgcgggatgg tgcttccgcg aagcggcact gtcaccatgt
     4441 tcttgatagc ggcagccttg cccttttcga cggcattggg cacctcgtta gccttgccgt
     4501 aacccatgcc gacgcgccca ttgcggtcac ccacgaccac taaggctgcg aagctgaaac
     4561 gacggccgcc cttgaccact tttgcacagc gataaatctg gattatgttt tcttccagtc
     4621 cgcctgcttt ttcaaactca tttgtgttct tctgcaaagc cacgggtaac tcctgacttt
     4681 agaattccaa gccgcccttg cgggccgcct ctgccaactc gtgaattcgg ccgtgatacg
     4741 gataaccgtt acggtcaaac acgatttttt ttattccctt ttcaacagcc tttgctgcca
     4801 gagcctcacc gacgacggct gcggcagttt tgtttttgcg gctgttgccg cctattttgc
     4861 cgacgatttc cttttcaagt gtggatgccg ccgcaagagt acgtccagtc tcatcatcta
     4921 tgagctgaac gtaaatatta tggttactgc ggaaaacgct cagcctcggt cgcagagccg
     4981 ttccctgaat ttttttacgc acacgccgtt tgcgacgctc gcgacgttcc tgtttcaaat
     5041 ctataggctt catcagattg cctcattaaa aattcattac cacgggccgt gtcatcggcg
     5101 acgctacgca cagccgccgg tagataaacc cgataaaaaa cctcagtcca ataaccgtta
     5161 gctgccgaaa gccttgccga ctttgcggcg aaccttttcg ccggtatagc ggacgccttt
     5221 gcctttatat ggttcaggcg gccttgtacg gcggcacttg gcggcaaatt cgccgacagc
     5281 ctgtttgtta acacctttta cagtgaattt tgccggctca ctatcgccgc gagcctgtgc
     5341 gttttcgact tcaacctgta tgccagccgg caatgccaga acttcaggat gacttttccc
     5401 gcagttgata gtaaaattct ttccctgaag ctgtacgccg tagccaacgc cgtaaacttc
     5461 caactttcgc tcaaaaccct tcgtcacgcc tgttatcatg ttattcacaa gcgccctggc
     5521 tgttccgtga agggcgcggc aggcctgctc gtcgtttttg cgatccacga cgacctggcc
     5581 ttctgcgact gtcactgtca cctgggggtt gaacgtaaaa ctgtcactgc caagtttgcc
     5641 cttgaccgta acggtagacc ctgcaacggc gacatctacg ccgccgggga tttctatcgg
     5701 tttcttgcct atacgcgaca ttctttcacc tgccttaaat cacttttagc agaccgtgca
     5761 gagcagttcg ccgccgacgt tcttctcgcg acattcgcgg tcgctgagta cgccaaagct
     5821 ggtcgaaacc acggaaatac ccatgccgtt aatcggacgg ggcagttcgc ctgccgacct
     5881 gtaaacgcgg cagcctggcc ggctgatacg cttgagttcg gtaacaacgt cttcgcccgt
     5941 cggtccgtat ttgagatata tcctcaacag gccctgcttg ttgtcttcaa tttttgtata
     6001 atcttcgaca ta
//
LOCUS       DAONFI010000124         5935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_15736_length_5935_cov_4.304932, whole genome shotgun sequence.
ACCESSION   DAONFI010000124 DAONFI010000000
VERSION     DAONFI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15736_length_5935_cov_4.304932"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PLK08_05215"
     CDS             complement(<1..1019)
                     /locus_tag="PLK08_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPS52935.1"
                     /translation="MGVILDIIYFFALLAAGPFLLFRMFRYGRYRHNWLQRFGRTPER
                     HGLQPVIWLHGVSLGEINASRTLVAELQSQLPDYRVVISSTTDTGMAAAEKAYSPFLT
                     VFQWPLDFTWAVRRAIRRTQPALVILMEGEIWPNFLAECNRRKIPAVVVNGRMSPNKG
                     YPRYKKLGLLAAALFNRLTAIGVQDEIYGHKFIELGGEPSKVHLTGMLKYDTIQVADS
                     VPGQEIIASQLALDTRAGKLLVAGGTGPGEEQILLEALGKKITGGELTLLIVPRKPER
                     FDEVARLITKAGFPCIRRSGHNTHATQLQNLPQRAVILGDTMGELRKFYGLSDMIFVG
                     RSLVH"
     gene            complement(1126..1398)
                     /locus_tag="PLK08_05220"
     CDS             complement(1126..1398)
                     /locus_tag="PLK08_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HPS52936.1"
                     /translation="MMTISVKEFQDFIKDKYYPTDSKRGTPGTFMWFIEEVGELATAL
                     AGNDQANREEEFADVFAWLCTLANINNVDLAKALEKYTKGDIKGFK"
     gene            1485..2264
                     /locus_tag="PLK08_05225"
     CDS             1485..2264
                     /locus_tag="PLK08_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPS52937.1"
                     /translation="MHESDIYSRQMLFSRIGRAGQDRLACARVAMVGMGGLGSWGAEL
                     LARCGVGYLRLIDCDVVEYSNLARQAMYTRRDADENIPKVTAAAKRLTEINPSCTVEP
                     VMARLDAANAGTLLSAFDVVLDATDDWQTRFVINSWCVANNVPWVFGGVVASVGQVMP
                     IMPHRTACLACIYDSPPDKPQEQANKASAVGVIGPAVAAVAALQTAQAVRIITGDKNN
                     STCLFHLDCWDLSTQKIVTEKPNPLCRVCGGRGEMQNECKR"
     gene            2248..3195
                     /gene="cysK"
                     /locus_tag="PLK08_05230"
     CDS             2248..3195
                     /gene="cysK"
                     /locus_tag="PLK08_05230"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPS52938.1"
                     /translation="MNVKDDITLTVGNTPLVRVRKTIPQTANIFAKMECMNPFSSVKD
                     RVAISMVDAAENAGLLVRGKGVIIEPTSGNTGIGLAMVAAARGYKCILSMPESMSIER
                     RKVLRALGCELILTPAAEGMTGAIRAAEEFVTQNPQAYMPQQFNNPANPQIHRLTTAP
                     EIWRDTDGKVDIFISSVGTGGTLTGVGEVLKKHNPAIKVIAVEPDASPVISQTLMGQT
                     PTPGPHMLQGIGAGFIPKVLNLNIIDEVIRVKNEDAFEYARKAARDEALFVGISSGAA
                     LKAAAVLAEREENRGKNIVAIMPSFGERYLSSPLFANID"
     gene            3192..4085
                     /locus_tag="PLK08_05235"
     CDS             3192..4085
                     /locus_tag="PLK08_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPS52939.1"
                     /translation="MNEKMKQLVDLHVHSNESDGSYSPADVIDFAVKAGVSAVALTDH
                     DTIAGLAAAACRAADYPQLSFIPGIEISAEYSPGIMHILGLGVNPTDAGLNAVASRLR
                     ESRANRNPQIIEKLRSLGVDISFDEVLEESRKDASPDAVLGRLHIAMVLVKKGFARNI
                     ADAFEKFLGNHAPAYVPKDHLLPAQAVAAINSAGGTAILAHPVQLECANELQLETLLR
                     KLMHVGLGGMECYHSSHNDVQTRTYLRLAQKLGLIITGGSDYHGLGKPDVAIGRPPVP
                     MSIVGEKNIMRWRGQAAGLPA"
     gene            complement(4045..4794)
                     /locus_tag="PLK08_05240"
     CDS             complement(4045..4794)
                     /locus_tag="PLK08_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52940.1"
                     /translation="MAHHQPPMPAYEQGGEKMFEKRLILLDMETQRDFFMPGGSFYSH
                     RAGEVRNNIYKLFDWAEDHHIPVLSSVLRVRQGELGPLGPKPHCVEATPGEQKLPRTL
                     QHPYIDLGIRNSTDLPDGIFDLYRQVIVEKRNPDLFMHARTERLFTELEGYHGVTFIA
                     CGANVDSGMTLTLIGLRARGFSVVLAADAVVSPGRWDAAELQIPRLYAKGVMFMKTSD
                     IVKTPAEAARDYFRNAKTLTPEDRLLVRAIA"
     gene            5189..5261
                     /locus_tag="PLK08_05245"
     tRNA            5189..5261
                     /locus_tag="PLK08_05245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5222..5224,aa:Ala,seq:cgc)
     gene            5449..5883
                     /locus_tag="PLK08_05250"
     CDS             5449..5883
                     /locus_tag="PLK08_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPS52941.1"
                     /translation="MNWGALNVWWVYALASAFFAALTAVLAKVGVKGIDSDLATAIRT
                     IVILIITWGIVFFKNGAGGLNSLSKNNVLFLVLSGIATGLSWLFYFRALQMGKVSHVA
                     AVDKTSVALAMILAVIFLHDALSLKLILGVLMVMAGTMIIIL"
BASE COUNT         1406 a         1560 c         1580 g         1389 t
ORIGIN      
        1 atgtgcacca gcgaacggcc gacgaatatc atgtcagaca aaccgtaaaa tttgcgaagt
       61 tcgcccatgg tgtcacccag aataacggcg cgctgcggaa ggttttgcag ctgcgtggca
      121 tgggtgttgt gtccgctgcg acggatacag gggaaaccgg ctttggttat caggcgggcc
      181 acttcgtcga accgctccgg cttgcggggg acaatcagca gcgtcagttc tccgcctgta
      241 atttttttgc caagtgcctc gagcaggatt tgttcctcac ccgggcccgt accgcctgcg
      301 accagcagtt tgccggctcg cgtgtccaag gcaagctgcg aggcgatgat ttcctggcca
      361 ggaacgctgt cggcaacctg aatcgtgtca tatttcaaca tgcccgtcag atgcactttc
      421 gacggttctc cgccaagctc aatgaatttg tggccgtaaa tttcatcctg cacgccgata
      481 gccgtcagcc ggttaaacaa cgctgcggca agcaggccca gctttttgta tctcggataa
      541 cccttgtttg ggctcatgcg gccatttacg acgacggctg gtatttttct gcggttgcat
      601 tcagccagaa aattcggcca gatttcacct tccatgagaa tcacgagggc cggctgcgta
      661 cgacgaattg cgcggcggac tgcccatgta aaatccagcg gccactggaa taccgtcaaa
      721 aatggcgaat atgccttctc cgccgccgcc atgccggtgt cggtcgtcga cgatattacc
      781 acgcgataat ccggcagttg cgattgcaat tccgccacaa gcgtgcggga tgcgttgatt
      841 tcaccgaggc ttacgccgtg cagccagatg accggctgaa ggccgtgacg ctcaggcgtt
      901 cggccaaaac gctgtaacca gttatgccgg tatcggccat atctgaacat ccggaaaagc
      961 agaaacggcc cggccgccaa caatgcgaaa aaataaatta tatctaaaat cacgcccatt
     1021 tttcatcaaa tccaaaagct gcaactgtca ttcatttaaa aactttttat atcgccatta
     1081 cgcatggctg ccagcgtagt cggtaaggca aaatatctgc aactgtcatt taaaaccttt
     1141 tatgtcgccc ttggtgtatt tttcgagggc ttttgcaagg tcgacattgt tgatattcgc
     1201 cagcgtgcac agccatgcga aaacgtcggc aaattcctct tccctgttag cctggtcgtt
     1261 gccggccaac gcggtagcca attcgcccac ttcctctata aaccacatga acgtccccgg
     1321 cgtaccgcgt ttcgagtcgg tcgggtagta tttatccttt ataaaatcct gaaattcctt
     1381 taccgatatc gtcatcatat catcctttgt ttaaacccca tattttcgct attttgactc
     1441 aatagtcaat tgactcttcc aatttactgc ctaaactgta acttatgcat gagtcggaca
     1501 tatattcgcg tcagatgtta ttcagccgca tcggccgggc tggccaggat aggctcgcct
     1561 gcgccagggt ggctatggtg gggatgggcg gcctgggctc atggggggct gagttgctgg
     1621 cacgttgcgg agtcggttat ctgcgtctga tagattgcga tgtggtggaa tattccaacc
     1681 tggccagaca ggcgatgtat acccgtcgcg atgccgatga aaatattcca aaagtgaccg
     1741 ccgccgcgaa acggctaacc gaaataaatc catcctgtac ggttgagccg gtaatggcgc
     1801 gcctcgacgc cgcaaacgcc ggaacgcttt tgagcgcatt tgacgtggtg ctggatgcaa
     1861 ctgacgactg gcaaacgcga ttcgtcatca attcatggtg cgtagccaat aatgtgccgt
     1921 gggtattcgg tggcgtcgtc gcctcagtcg gccaggtcat gccgataatg ccgcaccgta
     1981 cagcctgtct ggcatgtatt tacgattcgc cgccggacaa accacaggag caggcaaaca
     2041 aagcttcagc cgtcggcgtc atcgggccgg cagtggcggc tgtcgcagcc ttgcagacgg
     2101 cacaggctgt gcgcataatt acaggcgata aaaacaattc cacctgcctg tttcatctgg
     2161 actgctggga tttgtccacg cagaaaattg ttacggaaaa acctaatccg ctctgccgcg
     2221 tgtgcggcgg acggggagag atgcaaaatg aatgtaaaag atgatattac gttgacagtg
     2281 ggaaacaccc cgctggtcag agtccgaaaa accatcccgc aaaccgccaa catttttgcg
     2341 aagatggagt gcatgaatcc tttttcgtcg gtcaaggaca gggtcgcaat ttcgatggtc
     2401 gacgcagctg aaaacgccgg ccttcttgtt cggggcaaag gcgtgataat agaacccacc
     2461 agcggcaaca ccggcatagg cctcgccatg gtcgcagccg cccgcggata caaatgcatc
     2521 ctgtcaatgc cggagtccat gagtatcgaa cggcgaaaag tgctccgggc gttgggctgt
     2581 gagctgatac ttactccagc ggcggaaggc atgacaggcg cgattcgggc ggcggaggaa
     2641 tttgtaaccc aaaatccgca ggcttacatg ccgcagcagt tcaacaatcc ggctaacccg
     2701 cagatacacc ggctgacgac cgcgccggaa atatggcggg atacggacgg caaagtcgac
     2761 atattcatat caagcgtcgg cacgggcggc acgcttacag gcgtgggtga agtgctcaag
     2821 aaacacaatc cagccataaa agtcatagcc gtcgaaccgg acgccagccc cgtcatatcg
     2881 cagaccctca tgggccagac gcctaccccc gggccgcata tgctccaggg catcggcgca
     2941 ggattcatcc ccaaagtact taacctgaat ataatagacg aagtgattcg cgtgaaaaac
     3001 gaagacgcct tcgaatatgc ccgcaaggca gccagggacg aggcgctttt tgtgggcata
     3061 tcttccggcg cagcgttgaa agccgccgca gtcctggcag aacgcgaaga aaacagaggc
     3121 aaaaatatcg tcgccatcat gcccagcttc ggcgaacgat acctctccag cccgcttttt
     3181 gcgaatatcg attgaatgaa aagatgaaac agcttgtaga cttgcatgtt cattcgaatg
     3241 aatcggacgg ctcttattcg ccggcggatg tgattgattt cgccgtaaaa gccggcgtat
     3301 cagccgtcgc gcttaccgac cacgacacca tcgccggcct tgccgccgcc gcttgcagag
     3361 ctgccgatta tccgcaatta tcgttcattc caggcataga aatatcggct gaatattcac
     3421 cagggataat gcacatcctc ggactcggcg taaatccgac ggatgccgga ctgaacgccg
     3481 tcgcatcgcg gctgcgggag tcccgcgcga acaggaatcc gcaaataatc gaaaaacttc
     3541 gcagcctggg agtggatatt tcattcgacg aggttttgga agaatcacgc aaggatgcca
     3601 gccccgacgc cgtgctgggc cggctgcata ttgcgatggt gctggtgaaa aaaggttttg
     3661 ccagaaatat agcagacgca tttgagaaat ttttgggcaa tcacgctcct gcgtatgtgc
     3721 ccaaagacca cctcttgccg gcacaggctg tcgcggcgat aaacagcgcc ggcggcacgg
     3781 cgatacttgc ccacccggtt caactcgaat gcgccaacga gttgcagttg gagacgcttt
     3841 tacgaaagct catgcacgtc ggccttggcg gcatggagtg ctaccacagt tcgcacaacg
     3901 acgttcagac gcggacgtat ctgcggctgg ctcaaaaact ggggctgata ataacaggcg
     3961 gctcggatta tcacgggctt ggcaagcccg acgtagccat aggccgcccg cccgtgccga
     4021 tgagcattgt cggcgaaaaa aacatcatgc gatggcgcgg acaagcagcc ggtcttccgg
     4081 cgtaagcgtt ttggcgttgc ggaaataatc ccgcgcagcc tcagccggcg ttttgactat
     4141 gtcggacgtc ttcatgaaca tcacgccttt tgcgtacagc cgcggtatct gcaactcggc
     4201 tgcgtcccat ctgccaggcg acacgacggc atcggcggct aagacgacgc tgaaacccct
     4261 tgcgcgaagg cctatcagag tcagcgtcat gccgctatcg acattcgcgc cgcatgcgat
     4321 gaatgtcacc ccgtgatagc cttccagctc cgtaaacagc cgctctgtcc tggcgtgcat
     4381 gaagaggtcg ggattgcgtt tttcgacaat cacctgccgg tacaggtcaa atatgccgtc
     4441 cggcagatca gtgctgttgc gtatgcccag gtcgatatac ggatgttgca gcgtccgggg
     4501 cagcttttgt tcgcctggag tggcttcgac gcagtgaggt ttcgggccga gcgggccaag
     4561 ctcgccctgg cgcacgcgca gcacgctgct gagtacagga atgtggtggt cctctgccca
     4621 gtcgaaaagc ttataaatat tgttacggac ttcgccggca cgatgagaat aaaaacttcc
     4681 gcccggcata aaaaagtctc gttgagtttc catgtccagc agtatcaatc gcttttcaaa
     4741 cattttctca cctccctgct cataagccgg catcggcggc tgatggtgag ccattttttc
     4801 caatacatta tacgccctat agtgaggtaa ggtttcgata aaagttcagc ctttctgcaa
     4861 aaactttgtt tcgccggcaa aaacagctcc aaaaaaatgt aaaattgcta ttttttatat
     4921 aatttactca aagtggccga ttcgcgttat aaggctcggt aacgacttat gctgcaaaca
     4981 attacgacat gcgtgacaaa tagttctgcg atgtcgcctt tgtgagccgc ggtaatgtat
     5041 tttattgcaa aataagtctt ttgcttttta gcctcatatg cgaaaaatcg taaatcggtc
     5101 agtttgaata aattaaagaa ctttttatac aaaatacaaa aactgcttca caatttgccg
     5161 cacgatttgt atactttcca aacccttcgg ggccgtagct caattgggag agcgtcgcgt
     5221 tcgcaatgcg gaggttgagg gttcgatccc cttcggctcc atattttcaa caaagggcat
     5281 gttcggctga cgccgcgcat gccctttttt cattggtttt acgcccgacg tcaggataac
     5341 atatcggttg gcgtatttta taaatacaca cagccgccgg ctgattgcag gccgggggtt
     5401 ttttattcat aatatttttg cgatttgtca ttttgaaagg aaaaagacat gaattgggga
     5461 gcattgaacg tctggtgggt atatgcgctg gcgtcggcgt ttttcgcagc gttgacggcg
     5521 gtgcttgcga aagtgggcgt aaagggcata gactcggatt tggcgacggc gattcgcacg
     5581 atagtgatac tgattattac ttgggggata gtgtttttca aaaatggtgc gggcggtttg
     5641 aacagccttt caaaaaacaa tgtgctgttc ctggtgcttt ctggcatagc gactgggctg
     5701 tcgtggctgt tctatttcag ggcgctacag atgggcaagg tttcgcacgt agcagccgtc
     5761 gacaaaacca gcgtggcact ggcgatgata ctggcggtta tcttcctgca cgacgcacta
     5821 agcctgaaac tcatattagg cgtactgatg gtaatggccg gcacgatgat aattatcctt
     5881 tagagcatgt taaacttgat atgcgtcatt tctgctggct gcaacatcgc tctgc
//
LOCUS       DAONFI010000125         5856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16007_length_5856_cov_4.433029, whole genome shotgun sequence.
ACCESSION   DAONFI010000125 DAONFI010000000
VERSION     DAONFI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16007_length_5856_cov_4.433029"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..3192)
                     /gene="ppdK"
                     /locus_tag="PLK08_05255"
     CDS             complement(277..3192)
                     /gene="ppdK"
                     /locus_tag="PLK08_05255"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPS52942.1"
                     /translation="MAKQCKYVYSFGGGRSEGTAAMKALLGGKGANLAEMCSLGLPVP
                     PGFTITTEVCDLYYKLGRKYPVGLDRQIDDAIKKVEKVTGKTFGKGSNPLLVSVRSGA
                     AVSMPGMMDTVLNLGLNDQTLQAMIALTGNERFVWDSYRRFMQMFGDVVLGIEHHDFE
                     HALANVKAKRGVKLDTELCVDGLKEVVEAYKGVYRQNSKTFPQDPRQQLQASINAVFG
                     SWNNDRAIKYRQINDIRGLLGTAVNVQTMVFGNMGNDSATGVAFTRDPSTGSNRYFYG
                     EYLINAQGEDVVAGIRTPQEITRLGSLEWAKNNGIAEDVRVSKYPSLEETLPKCFREL
                     DKIRKILEHYYKDMQDLEFTIEQGKLYMLQTRNGKRTAAAAVQIAVDLVKEKLITEKE
                     AVCRIEPKALDQLLHPVFDVTSEKSAKQITKGLPASPGAATGQVYFSAAEAEKQAAAG
                     KKVVLVRLETSPEDIGGMNVSEGILTGRGGMTSHAAVVARGMGRCCVAGCEGMVIDYA
                     AGQFTAGGCTVREGDWISINGSTGAVYAGKVDTVAPQLSGPFGTIMKLSDKYRKLGIR
                     TNADTPRDTAVAVKFGAEGIGLCRTEHMFFDGDRIISMRKMILAEDAAGRKEALTELL
                     PLQKSDFTAMFKELQGRPAIIRFLDPPLHEFVPHDVAGQQEMADAMGISLEKVKEKVA
                     SLHEFNPMLGLRGCRLGITFPEITEMQARAVIEAAYEVPGSKPEIMIPLVGNVKELEN
                     QKKLILAVYEEIKTQMKRPLKDLKIGTMIEVPRGAITADEIAQQAEFFSFGTNDLTQM
                     GCGFSRDDAASFLKQYVEMGIYEYDPFVTLDRDGVGKLVKTAVILGRSTRPNIKLGIC
                     GEHGGDPKSIEFCHMVGLNYVSCSPYRVPIARLAAAQAAIRYEHGCAIMCNCESHAKA
                     AKKAAAKKPAKKIAKKPTAKKAVAKKAVAKKTVAGKKTARKAAPKKVVKKTVKKNKK"
     gene            3547..4008
                     /locus_tag="PLK08_05260"
     CDS             3547..4008
                     /locus_tag="PLK08_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS52943.1"
                     /translation="MAEYLTSFRGEVSRLVRRELKKTFAQLKAENVKLKKHVADLRKK
                     MAGLQKANRYIEKETKYVARLREEKEAASENPQIDRMRIKGSAIRKLREKLGISQAEL
                     AALLGVTGQTIYQWERRQNESLRRLRNDSKIALLEIRKMGKKQVLAKLEAM"
     gene            4143..>5856
                     /locus_tag="PLK08_05265"
     CDS             4143..>5856
                     /locus_tag="PLK08_05265"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPS52944.1"
                     /translation="MIYRVEIWARDGFGDPHAAGVLSQIKELGIDCVTAVKHARIFFL
                     GGRLSQSDVEKISAGLLVDPVTESYRIGQTPDKGKCVVEVHLQPGVMDPVAESCIKAI
                     GDMGFDIQTVSTARRYELTGDVSDEQRETIARRLLANAVIEDTYFKGHTPSALNLGKY
                     KLKIIEVPIRNLDDAGLMELSKKGDLFLNLTEMKAIRNYFRKLGREARDIELEMIAQT
                     WSEHCGHKTFRSDVKVRNSAGEIVEEIPNLLKSTVFKSTMELNKPWCLNVFADNAGVI
                     DFDDDTAVCFKVETHNHPSAIEPYGGSMTGIGGVVRDPLGTGLGAKPIANTDVFCFGD
                     TTMKLSDVPTGVLHPKRVMRGVVSGVRDYGNRMGIPTVNGAVYFDERYLGNPLVFCGT
                     IAVMPKNKCFKQHPKEGNAIVCLGGRTGRDGIHGATFSSGELTHKHETKFSHAVQIGN
                     AITEKKVLDVLMQARDRNLFDCITDCGAGGLSSAIGEMGEKIGAEVHLDRVPLKYAGL
                     SYSEIWISEAQERMVLSVPQENVDEILKLFASENVEAQVLGRYGVRDDDGHPERLPGL
                     VRVEV"
BASE COUNT         1425 a         1514 c         1536 g         1381 t
ORIGIN      
        1 atgcagcctg acgcaacaga gcgaagttgc agccagcaga aatgacgcat atcaagttta
       61 atatgctcta gcctgccctg ttaagattta caggccagct actctcactg cttcgcagtg
      121 ccgtttttgc aggcctccta ataatatatg caatatttta cacaagttat acttgacgca
      181 cttatttttg aaaattatat gcctctgaaa agagacaaaa aaagcccggc cgtttttaat
      241 cagccgggct ttttgcgttt ttaaaacagg gttgttttat ttcttattct ttttaacggt
      301 ttttttaaca accttttttg gcgctgcttt cctggcagtc ttttttccgg caacggtttt
      361 tttagccaca gcctttttgg ctaccgcttt ttttgccgtc ggttttttgg cgattttctt
      421 tgccggcttt tttgcagcag ccttttttgc ggctttggca tgcgattcgc aattgcacat
      481 tatcgcacag ccgtgttcat agcggatggc ggcttgcgcg gcggcaaggc gagctatcgg
      541 cacacggtac gggctgcacg atacgtaatt gaggcctacc atatggcaga actcgatgct
      601 cttcggatcg ccgccgtgtt cgccgcaaat gcccagcttg atattcggtc gggtcgaacg
      661 gccgaggatg acggctgtct tgacgagctt gcctacgccg tcacggtcaa gcgtgacgaa
      721 cgggtcgtac tcgtaaatgc ccatctccac atactgcttg aggaacgacg cagcgtcatc
      781 gcggctgaat ccgcagccca tctgcgtaag gtcgttcgtg ccgaagctga agaactcagc
      841 ctgctgtgcg atttcgtcgg ctgtgatagc cccgcgaggc acttcaatca ttgtgccaat
      901 cttgaggtct ttgagcggcc gtttcatctg tgtcttgatt tcttcgtaaa cggcaaggat
      961 gagttttttc tggttttcca gctccttgac attgccgacg agcggaatca tgatttccgg
     1021 ctttgagccg ggcacttcgt aagccgcttc gatgacggca cgagcctgca tttccgtgat
     1081 ttcagggaaa gttatgccca ggcggcagcc gcgaaggccg agcatgggat tgaactcgtg
     1141 caacgaagcg actttctcct tgaccttttc aagcgagatg cccatagcgt cagccatttc
     1201 ctgttggcca gccacgtcat gcggcacaaa ttcgtgcaac ggcgggtcga ggaagcggat
     1261 tatcgccggc cggccctgca gttccttgaa cattgccgta aaatcgctct tctgcagcgg
     1321 cagaagttcg gtaagcgcct cttttcggcc agctgcgtcc tcggcaagaa tcatctttcg
     1381 catggagatg attctgtcgc cgtcgaagaa catgtgttcg gtacggcaaa ggcctatgcc
     1441 ttcggcgccg aatttaacgg caacggcggt gtcgcgcggc gtatcggcgt tggtgcgaat
     1501 gccgagcttg cggtatttgt cggaaagttt cattatagtc ccgaacggcc ctgagagctg
     1561 cggagcgacg gtgtcgacct tgccggcata cactgcaccg gtcgaaccgt ttatgcttat
     1621 ccagtcgcct tccctgacgg tgcatccgcc ggctgtaaac tggccggcag catagtcgat
     1681 aaccatgccc tcgcagccgg caacgcagca cctgcccatg ccgcgtgcaa ctacggcagc
     1741 gtggctggtc atgccgccac ggccggtgag aatgccttcg gatacgttca tgccgccaat
     1801 gtcctccggc gaagtctcaa gccggacgag cacgactttt ttgccggctg ccgcctgctt
     1861 ttccgcctcg gccgcactga aatatacctg gccggtcgcg gctcctggcg aagcgggcaa
     1921 acctttggta atctgtttcg cagatttttc agaagtaacg tcaaaaaccg gatgaagaag
     1981 ctggtcgagg gctttgggct ctatacggca gaccgcttct ttttcggtaa tgagcttttc
     2041 cttcacaagg tcgacggcta tctgcacggc tgccgcggct gtgcgcttgc cgttgcgggt
     2101 ctgcaacatg tacagcttgc cctgctcgat ggtgaattcg agatcctgca tgtccttgta
     2161 ataatgttca agaattttgc ggattttgtc cagttccctg aaacatttgg gcaaggtctc
     2221 ttcgagagac ggatatttgc tgacgcggac atcttcggca atgccgttgt ttttggccca
     2281 ctccagtgaa ccgagcctgg tgatttcctg cggcgtacga atgccggcta cgacgtcctc
     2341 accctgggca tttatgagat actcgccata aaaatacctg ttcgagccgg tggaaggatc
     2401 gcgggtaaac gcaacgcctg tggcggaatc gttacccatg ttgccgaaaa ccatcgtctg
     2461 cacgttgacg gctgttccca gcaagccgcg aatgtcatta atctgacgat attttatggc
     2521 gcggtcgtta ttccaactgc cgaaaacggc attgatagac gcctgaagct gctgacgcgg
     2581 atcctgggga aaagtcttgc tgttctggcg atagacgcct ttgtatgctt caacgacttc
     2641 tttgaggcca tcgacgcaga gttccgtatc cagttttacg ccgcgcttgg cttttacgtt
     2701 cgccagggcg tgttcaaaat cgtgatgttc aatgccgagc accacgtcgc cgaacatctg
     2761 catgaagcgg cggtaggagt cccacacgaa ccgctcgttg ccggtcaggg caatcatcgc
     2821 ctgcaacgtc tggtcattca agccgagatt cagaaccgtg tccatcatgc cgggcatgga
     2881 caccgccgcg ccggaacgga cggaaaccag cagcggattg ctgcctttgc caaaagtttt
     2941 gcccgtaact ttttcaactt tttttatggc atcgtcaatc tgacgatcaa ggccgacagg
     3001 gtatttccgg ccgagcttgt aatacaaatc gcaaacttcg gtagttatgg taaaaccagg
     3061 cggcacaggc agacccagtg aacacatttc tgcgagattc gcacctttcc cgcccagaag
     3121 ggctttcatg gcggcagtgc cctcgctcct gcctccgcca aaggaatata catacttgca
     3181 ttgctttgcc atctaaactt ctcctaatta taaaagtctc gtgatttgta tctaaaagac
     3241 aaactgtcaa tcaatacttc aagccggaat ccataatcct gctgaaaaac aaagaaaaac
     3301 cctcacgccc atgatttgcg ttcggggtag tagtgtagaa ctttctctat tgcaagtcaa
     3361 ctgcctcgtc ggcgaaaaat gcaatattta ttttgagttg gcctctattt ttttatatac
     3421 tcgtcggctc ggaaaacatt atcacatatt tgacgattac attcttttca gccggcgact
     3481 gccgtttaat aaatatatga taatagttag gttttggttc ttacaaagtt tgcaggagaa
     3541 tttaaaatgg cagaatatct gacatcattt agaggtgaag tgtcaaggct ggtacgcagg
     3601 gaacttaaaa aaactttcgc acaattaaaa gcggaaaacg tgaaactcaa aaaacacgtt
     3661 gccgacctgc gaaaaaaaat ggctggattg cagaaggcga accgttacat cgaaaaagag
     3721 acgaaatatg tcgccagact gcgtgaagaa aaagaagcag ccagtgaaaa tccacagatt
     3781 gaccgcatgc gaatcaaagg cagtgcgata cgcaaactgc gtgaaaagct cggcatttca
     3841 caggctgagc tggctgcgct gctgggcgtg accggtcaga ccatatatca atgggaacgc
     3901 cggcagaacg agtcgctccg gcggctgcgc aatgattcca aaatcgccct tctcgaaatt
     3961 cgcaagatgg gcaaaaaaca ggttctcgcc aaacttgagg ctatgtaaac actcatcgct
     4021 ctaagtttgc cgcggctgaa aacgtaagtt cagaatcgga aatctgaaaa acaaaaccga
     4081 aattgaaaat cggaaattag aaaccataag cagattcaaa atcggaaatc aggaagttga
     4141 aaatgattta tcgggtagag atatgggcca gggacgggtt tggagatccg cacgcagcgg
     4201 gcgtattgtc acagataaaa gaactcggga tagattgcgt tactgcggta aaacacgcca
     4261 ggatattttt cctcggcggc cggctttcgc agtcggatgt tgaaaaaata tcagccggcc
     4321 ttctcgtaga ccccgtaacc gaatcataca gaatcggcca gacgcccgac aagggaaagt
     4381 gtgttgtaga agttcatctt cagccgggcg ttatggaccc cgtagcggag tcatgcataa
     4441 aagccatcgg cgatatgggc tttgacatac aaaccgtttc aactgcgcgg cgatatgaac
     4501 ttacgggaga cgtcagcgac gaacagcgtg aaaccatagc ccgccggctg ttagccaacg
     4561 ctgttataga ggatacctac tttaaaggcc atacgccatc ggcgctgaac ctcggcaaat
     4621 acaaactgaa aattatcgaa gttcccatac gcaatctcga cgacgccggc ttgatggaac
     4681 tcagcaaaaa aggcgacctg ttcctgaatc tcacggagat gaaggcaatc aggaactatt
     4741 tccgcaagct gggccgtgaa gcccgcgaca tagaactgga aatgatagcc cagacatggt
     4801 cggaacactg cggccataaa actttccgca gcgacgtgaa agtcagaaac tcagccggcg
     4861 aaatcgtgga agaaatcccg aatcttctca aaagcacggt attcaaatcc actatggaac
     4921 tcaacaaacc ctggtgtctc aacgtctttg ccgacaatgc gggcgtaatc gacttcgacg
     4981 acgatacagc cgtctgcttc aaggttgaaa cgcacaacca tccatcagcc atcgaaccgt
     5041 acggcggcag catgacgggc ataggcggcg tcgtgcgtga cccgctgggc acaggtctcg
     5101 gcgccaaacc catcgccaac acagacgtat tctgtttcgg cgacacgacg atgaaacttt
     5161 ccgacgtacc gaccggcgtg cttcatccca agcgggtgat gcgtggcgtc gtaagcggcg
     5221 tgcgagacta cggcaatcgc atgggcatac cgacggtaaa cggcgcggta tatttcgacg
     5281 agcgatatct gggcaatccg ctggtgttct gcggcacgat agccgtcatg cccaagaaca
     5341 aatgtttcaa gcagcatccg aaagagggca acgccatcgt ctgcctgggc ggccggacgg
     5401 gccgagacgg cattcacggt gcgacgttct ccagcggcga actcacgcat aaacacgaaa
     5461 cgaaattctc gcacgccgtg cagataggca atgcgataac ggaaaagaaa gtgctcgacg
     5521 tcctgatgca ggctcgcgac aggaatctgt tcgattgcat taccgactgc ggcgcgggcg
     5581 ggctcagcag cgcaatcggc gaaatgggcg aaaaaatcgg cgcggaggtt catctcgacc
     5641 gcgttccgct caaatatgcc gggttgagtt attcggaaat atggatttcc gaggctcagg
     5701 agcgtatggt actgtccgtt ccgcaagaga acgtagacga aattttaaaa cttttcgcaa
     5761 gtgaaaatgt cgaggcgcag gtattgggcc gttacggagt aagggacgac gacggccatc
     5821 cggagcgcct ccccgggctc gtgcgtgtgg aggtcg
//
LOCUS       DAONFI010000126         5836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16093_length_5836_cov_4.066424, whole genome shotgun sequence.
ACCESSION   DAONFI010000126 DAONFI010000000
VERSION     DAONFI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16093_length_5836_cov_4.066424"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLK08_05270"
     CDS             complement(<1..1027)
                     /locus_tag="PLK08_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52945.1"
                     /translation="MDVRKFKVPAMAGMAVVVAIIAGMVVFGGGRKKPLPAIMAAPAE
                     SKNLFVEQVEDNEDVGRIEPLAKPQTQAVVQDADSVVPLTKNESRTIEPVAVTPIAKN
                     SEPAYSQNQKLQKKSGLSENVKSAEPTAAKAEETPKVQVTTAAEIAAVEAEKVQMPVM
                     APVAAVAVKNTSEPDFAGSKAMEDAAATAAVERKAEPAAKPALDDIKIVEDAPAAAVA
                     EKSPQPAKAVAASDAIVSAQNTEPQIAAISTAAETKKNSPQAENMIEDSPEVVKTEAR
                     PKADWFAAPADASDGTSRLESNSFTLDGTPGGFITPKAYLINPGRDGQLFGLPAVGVR
                     FTSLGTKD"
     gene            1751..2464
                     /locus_tag="PLK08_05275"
     CDS             1751..2464
                     /locus_tag="PLK08_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS52946.1"
                     /translation="MDTNKDNKYLVSAAGLHKTYHLGKTSLEVLRGVSLEIGHGEFVA
                     IYGHSGSGKSTLLHLLGLLDEPSKGSIAFDGYEVAKTSARKRNHMRCRDIGFVFQFYY
                     LLPELTVLENTLLPGMVDNSSFQWLKNRGELKSRACDILAELGLGERLKHRPKELSGG
                     ERQRVAIARALLHAPKLLLADEPTGNLDSKTGRKIMDLLLKLNREKKQTILMVTHDNE
                     LAGLVDRCLYLQDGVFVKE"
     gene            2484..3212
                     /gene="kdsB"
                     /locus_tag="PLK08_05280"
     CDS             2484..3212
                     /gene="kdsB"
                     /locus_tag="PLK08_05280"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPS52947.1"
                     /translation="MSAVIIIPARYASTRLPAKPLLKETGKFLIQHVVENVRNTPDIS
                     RVVVATDDKRIFDAVESFGADVVMTRADHTSGTDRLAETASLLGLADDDIVINVQGDE
                     PNMPPAVITVLLNLLKNCDAPMATLCTPLSPQAAADPNKVKVVFDQHNYALYFSRAKI
                     PFDRDGHAAAEYFLHMGIYAYRVSFLKKFTSLPQTKLEKTEKLEQLRALENGYRIRIA
                     AVDYHGVGIDTPQDYAEFVASRRK"
     gene            3313..3395
                     /locus_tag="PLK08_05285"
     tRNA            3313..3395
                     /locus_tag="PLK08_05285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3346..3348,aa:Leu,seq:taa)
     gene            3597..4076
                     /locus_tag="PLK08_05290"
     CDS             3597..4076
                     /locus_tag="PLK08_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPS52948.1"
                     /translation="MDSDAKKFYFEIFTPDGIRFQGDVNSVTLPAIDGYMGILAGRAP
                     VTAIVTTGTVSIQKTDGGTEENWFVSHGFCQMSQNHLTLLAEESKPLAELDPEHAWEL
                     LQRAYKMPKDTTEQRTIRDQVIHNCRIRFNLAQKAKKAKAGGMMDFNSLMSPGGKTE"
     gene            4080..5000
                     /gene="hemC"
                     /locus_tag="PLK08_05295"
     CDS             4080..5000
                     /gene="hemC"
                     /locus_tag="PLK08_05295"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPS52949.1"
                     /translation="MPPILKIATRSSRLAVAQANSIADEIRRSGFFNGEIQILTLTTR
                     GDVTDGPLWQSGGKGLFTAELETALRAGRADLAVHSAKDLPAQTPEDLPLIAVPRRLD
                     PRDALITRAAASLSDIQHGGVVGTSSLRRRAFVHQIRPDLKTVPLRGNVETRLDRVQN
                     GEISAAVLAMAGLLRGGFVSERPELKITPLDVETFIPAPGQGCLAIQAAAGRDDVAQI
                     LKNVNHRESHEALDAEREILRQLGVSCHSCFAVHIRKVGRYWQGLAAAAREDGNAMIH
                     TEHTGNSPVNLIPLIVDNLVSLGIKKLLTW"
     regulatory      5134..5325
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5504..>5836)
                     /locus_tag="PLK08_05300"
     CDS             complement(5504..>5836)
                     /locus_tag="PLK08_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52950.1"
                     /translation="MKQFFREAVFRAILKRNSFPAEAVEKATLLLLSMQTDDGSGTFI
                     KLKMEFFEKCSNTLMPVYWELSIGDPFPAERGDIMRNEKPHALLRLASSIFLRRHLRT
                     DFIQTLLH"
BASE COUNT         1457 a         1554 c         1454 g         1371 t
ORIGIN      
        1 tgtctttagt tcccagcgac gtgaatcgca cacccaccgc aggcaggccg aaaagctggc
       61 cgtccctgcc ggggtttatc agatatgctt tcggcgtgat aaaaccgcca ggcgtgccgt
      121 caagcgtgaa ggaatttgac tccagccggc tagtcccgtc tgatgcgtct gccggcgcgg
      181 caaaccagtc agccttcggc cgggcttccg ttttgacgac ttccggagaa tcttcaatca
      241 tgttttcagc ttgtggcgaa ttttttttag tttccgcagc tgttgatata gcggcaatct
      301 gcggctcagt attttgagcc gacacaatag cgtctgaagc ggcgacagcc ttcgcaggct
      361 gaggcgattt ttcggcaaca gcagcagccg gcgcatcttc gacaattttg atatcgtcca
      421 gcgcgggttt ggcggcaggt tcagcctttc gctcgacggc ggcagtggcg gcggcatctt
      481 ccatcgcctt tgagccggcg aaatccggct cagacgtatt ttttacagcc acagccgcta
      541 caggcgccat aacaggcatc tgaacttttt ctgcttctac ggcggctatt tcagcagccg
      601 tcgtcacttg aacttttggt gtttcttcag ccttcgcggc agtcggttct gccgatttga
      661 cattttctga caggccggat tttttctgca atttctgatt ctgactataa gcgggttcag
      721 agttttttgc aatcggcgtc accgcgacgg gttcgatggt acggctttca tttttagtca
      781 gcggcacaac gctgtccgca tcctggacga cagcctgtgt ctgcggttta gccagcggtt
      841 ctattctgcc gacatcttca ttatcctcca cctgctcgac gaacaggttt ttcgattccg
      901 ccggcgcagc cataatagcc ggcaggggct ttttcctgcc gccaccaaaa accaccatcc
      961 ctgcaataat ggcaaccacg acggccattc ccgccatcgc gggtacttta aactttcgta
     1021 catccatgct gcattccttt aaaatcaatg cactacttaa ttgagtgcag tttcaatttt
     1081 acgcgggcat aaaaaaacct cgtcctgagg caaatgaacg gcaacaacgg gggaacatcg
     1141 gcatccctgc ctgtctcaca cgtcctcata cgagggttgt tcagcaatcg tcggcatcct
     1201 gccggcagtt tttattattg cttttccatt ccgaatccct gcggattcgt acgtcacaac
     1261 aggctgatag atatacactt attttttctc atgtcgagtc aaaaacagcc gtaaaataag
     1321 gctaaaaccg gtatcgaaaa ttatcaacaa cccgccagcc cgccagccgg aattccacgg
     1381 ctgaatattc ggaacgctcc aaaccagcct aaattccgat agttgtgaaa atgacggcgt
     1441 aaccgaatag gaattatcaa caactcccaa cagctgttga taaaactaat atctaccgct
     1501 aaaatttttt tcgagaatag ccttattgcc gcattagagc ggttctcaaa attattttcc
     1561 ttttgaagct tgctgcaagt ttccctgcta ttgtccgtct gcatcggaaa ccatgcaggt
     1621 tgcctgcttc gacgtcctta gcaggaaaac tttcgctgac gctcaaaaag aaaattcttt
     1681 agagaaccac tctatgtgaa aaatgaaatc tcaatcgaaa atcacggctg atgattgtat
     1741 aatccctgca atggacacaa ataaagacaa taaatatctc gttagcgcag ccggccttca
     1801 caagacgtat catctcggca agacgtcgct ggaggttttg cgtggcgtat cgctggaaat
     1861 cgggcatggt gaatttgtcg cgatatacgg ccacagcggt tcgggcaaga gtacgttact
     1921 gcatctgttg gggcttttgg acgaacccag taagggcagc attgcattcg acggctacga
     1981 agtcgccaaa acgtccgctc gcaagcgcaa tcatatgcga tgccgcgaca tcgggttcgt
     2041 ctttcagttc tattatctgc tgccggagct aaccgtgctg gaaaacacgc ttctgcccgg
     2101 catggttgat aattcatcgt ttcaatggct gaaaaaccgc ggtgaactta aatcgcgtgc
     2161 ctgcgatatc ctcgcggaac tcggcttggg agaacggctg aaacaccggc ccaaggaact
     2221 gtccggcggc gagcggcagc gggtagccat cgcccgcgcg ctgcttcatg cccccaaact
     2281 cctgctcgca gatgaaccga caggcaatct cgacagcaaa accggcagaa aaatcatgga
     2341 tttgcttctg aaactcaaca gggaaaagaa gcagaccatt ttaatggtca cgcacgataa
     2401 tgaactggcc ggccttgtcg accgctgtct gtacctgcag gacggcgtat ttgtaaagga
     2461 ataaattgga gattttttat aagatgagtg cagtaatcat aattccagcc agatatgcat
     2521 cgacgcggct gccggcaaaa ccgcttctga aagaaacggg caaattcctc atacaacacg
     2581 tcgtcgaaaa tgttcggaat acgcctgaca tttcccgcgt cgtggttgcc accgacgata
     2641 aacgcatttt cgacgcggtt gaatcattcg gcgcagacgt cgtaatgacg cgagccgacc
     2701 ataccagcgg cactgacagg ctggcggaaa cagcttcgct cctgggcctg gcggacgacg
     2761 acatcgtaat caacgtccag ggcgacgaac cgaatatgcc gccggcagtg ataaccgtat
     2821 tgctgaatct gctaaaaaat tgcgacgcgc ctatggctac gctgtgtacg ccgctgtcac
     2881 cgcaagccgc cgctgacccc aacaaggtca aagtcgtctt cgaccagcat aattacgcac
     2941 tctatttttc acgcgcaaaa atcccattcg accgcgacgg ccatgccgcc gccgaatatt
     3001 tcctgcacat gggcatatac gcttaccgcg tgtctttttt gaaaaaattc acgtcgctgc
     3061 cgcagacaaa attggaaaaa acggaaaaac tcgaacagct ccgcgcattg gagaacggtt
     3121 atcgcatacg aatcgcagcc gtcgattatc acggcgtcgg aattgatacg ccgcaagatt
     3181 atgccgaatt tgttgcatct cggcgtaagt gaacttgtaa cgcgacgtaa aatgtatata
     3241 atccctgtac atcggctgct gttgtttgca tacggaataa tctctgcaaa atttcggcag
     3301 ccggcggaaa gtgccggagt ggcggaatgg cagacgctgg ggatttaaaa tcccctttcc
     3361 ttcgggaagt gtgggttcga gtccctcctc cggcatccac tgcgtggagg ctgaatatat
     3421 ccagccgacg ttttttggga aacttaacct ggccggacga gaacacaccg cggattttgc
     3481 gaagcaaaag acgcttttgt gcggttcgac gccgaagccc agcaacgcga agcgttgcgt
     3541 agcgaaggcg ccgcgaagcg agtcctcctc cggcatccac tgcgtggagg ctgacggtgg
     3601 attcagacgc gaaaaaattt tatttcgaaa tattcacgcc ggacggcatc cgtttccagg
     3661 gcgacgtcaa cagcgtgact ctgcctgcga ttgacggtta catgggcata ctcgccggcc
     3721 gtgcacctgt gacagccata gttaccaccg gcacggtctc aattcagaaa actgacggcg
     3781 gcacagaaga aaactggttc gtctcgcacg gtttctgcca gatgagccag aatcatctca
     3841 cgctgcttgc ggaagaatca aaaccgcttg cagaactgga tccggaacat gcgtgggaac
     3901 tgttgcagcg tgcgtacaaa atgcccaaag ataccaccga acagcgtacc atacgcgatc
     3961 aagtcatcca taactgtcga atccgcttta acctggccca gaaagccaaa aaggcaaaag
     4021 ccggcggcat gatggacttc aacagcctga tgtctccagg cggcaaaaca gaataagcca
     4081 tgccacccat attaaaaatc gcgacccgct ccagtcgcct ggccgtcgcc caggctaatt
     4141 ccatcgcgga cgaaatccgg cgcagcggtt tttttaacgg cgaaattcaa atactcaccc
     4201 tgactaccag gggcgacgtg accgacggcc cactttggca gtcaggcggt aaggggttgt
     4261 ttacggctga acttgaaacg gctttgcgcg ccggccgggc tgacctcgcc gtacattcgg
     4321 caaaagacct gccagcacag actcccgaag atttgccttt aattgccgtg cctcgccgcc
     4381 ttgacccacg agacgccctt atcaccagag ccgccgcctc gctgtcggac attcaacacg
     4441 gcggtgtcgt cgggacgagt tctttacgcc gccgcgcgtt tgtgcatcaa atcaggcctg
     4501 acctgaaaac cgtgccgtta cgcggcaatg tcgagacgcg gttggacagg gtgcaaaacg
     4561 gagaaatatc ggctgccgtc ctggcgatgg cgggcctgct gcgaggcgga ttcgtatcag
     4621 agcggccaga actcaaaata acgccgctgg acgttgaaac gttcattccg gctcccggcc
     4681 agggctgtct tgcaattcag gcggcggctg gcagagacga cgtggcacaa attcttaaaa
     4741 acgtcaatca ccgcgaatct cacgaagcac ttgatgcgga acgcgaaatc ctgcggcagc
     4801 tcggcgtaag ctgccatagc tgttttgcag tgcatatccg caaggtcggc agatactggc
     4861 agggcctggc tgccgccgca cgcgaagacg gcaacgccat gattcatacg gaacataccg
     4921 gaaattcacc cgtaaatctc ataccgctga ttgtggacaa tctcgttagc ctcggcatta
     4981 aaaaactgct gacttggtaa tcttttttaa aaagttaatc tctacgattt acagttgtcc
     5041 tcaatatagc ggttattttc gaaagccgtt ataaaaaccg tagaattgcg aatgtcatat
     5101 aatttttctg ttgacataaa agttttttgt gatagttatt atttcgttac aggtttcctg
     5161 aaaaggaacc aaaagggaag gccgtgtaaa tcggccgcgg acgcgccgct gtaaccggtg
     5221 acaacttttg caatagccac tgttccgctg tggatgcggg atgggaagga gcaaaagaag
     5281 actgacccgg aagccagaag acctgcctgt aatcgcctga aaatgactct cgcgtaatgg
     5341 agtcggcaag aattccttta tggattttca cgccttttcg ccattccgag agccggaaaa
     5401 ttaacggcta tccgtaaaaa tgacgtcacg caaagaaaac ggatatccta aattctccaa
     5461 tcaggttgtt gccggttgcc ttgcgctttg ggccgccgtc atttcaatgt agaagcgtct
     5521 gaataaagtc ggtacgcaaa tgtcgtcgca gaaaaatgct gctcgcaagc ctcaggagag
     5581 catgaggctt ttcattccgc atgatatctc cgcgttccgc agggaagggg tcgccaatgg
     5641 atagttccca atagaccggc attagggtat tagagcattt ttcaaaaaat tccattttga
     5701 gtttgatgaa agttccactg ccatcgtccg tctgcattga caataaaagc agagttgcct
     5761 tttcgacggc ctcggcagga aaagaatttc ttttgagaat cgctctaaat actgcttctc
     5821 taaaaaactg tttcat
//
LOCUS       DAONFI010000127         5832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16106_length_5832_cov_4.300502, whole genome shotgun sequence.
ACCESSION   DAONFI010000127 DAONFI010000000
VERSION     DAONFI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16106_length_5832_cov_4.300502"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2773
                     /locus_tag="PLK08_05305"
     CDS             <1..2773
                     /locus_tag="PLK08_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPS52951.1"
                     /translation="RIRTFPEDAEDPEVTLLTHRREVLSLVIYGNTSPQVLHELAERT
                     RDELLQRKDITQVEIVGVKALEIKIEIPQENLRRYNLTIDQVAQRIKAAAVEIPGGGL
                     KTRAGEILVRVKERKDYGRQFAVLPIISTPDGSQVKLEDIAKIDDGFNEDSDTQAVYD
                     NKPAVGIDVYSVGEQTPMQISKSVAEFKETLDTQLPPGIRSVILDDKSVVFKQRIDLL
                     VSNGVSGLILVMITLGLFLEIRLAFWVMMGIPVSFMGSMLVMPLLGVTINMMSLFAYI
                     IAIGIVVDDAIVVGENIYHYRQKGKSFYEASILGVGDVAMPVTFSVLTNCVTFLPLYF
                     MPGMMGKVFNMIPLVVITVFLISLLESIFVLPSHLGHQKEWKLRGLFGWIHHRQQDFS
                     HWFTTWINCRYGPFLDKAIHWRYTTLGVGVAILLCTIGYAVSGRMGFALFPKVESDYA
                     YATITLPYGVAFIKTEKISDRLFNAAQKVIKESGHAELVEGIFTVLETNSVTMKITMA
                     QPEIREKIMSTAEFTNRWRETTGTLLGIENAKFESDRGGPGSGDALTIELSHRDMKVL
                     EKASAELAADLRKYPMVSDIDDGFSPGKEQLDFTIKPEGKSLGLTANEIARQVRSSFY
                     GAEAIRQQRGRNEIKVMVRLPKNERSSEHNVEDLMIRTPDGIEVPLREVANFTRGRAY
                     TTIDRRNGRTIVNVTADVTPQSRAGEIIRSLIQNELPALMAEYPGLAYSFEGKQADQR
                     ESLGNLQVGFLLSLLVIYAMLAIPFRSYLQPLIVMVSIPFGIVGAVLGHIIMGYTLSV
                     VSLLGIVALAGVVINDSLVLIELANKLRKDNALRNPAEAIHAAAIGRFRAIILTTLTT
                     FFGLAPIICEQSRQARFLIPMAISLGFGILFATFITLILVPALYVMIEDMKNIYAGFF
                     KRKKSSNVA"
     gene            2763..4136
                     /locus_tag="PLK08_05310"
     CDS             2763..4136
                     /locus_tag="PLK08_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPS52952.1"
                     /translation="MSLEQAHRLEHEKIPQLLLRFSIPSTVATVVHGTYNVVDRIFVG
                     KAVGEEALAALAVAMPVMMIMMAFGMMFGRGGSALISIHMGEKRFDKAERVLGTGLLL
                     FCILSFLFTCFGLIYIDPLLRIFGANDAIMPMAREYLSIILWGSLFHEISFGVNNYIR
                     SEGNAKTAMMTAFFAAGLNVVLDPIFLFVFNMGVAGVAWATIIAQFASAVWVMLYYFG
                     GKSLLRFHLKNLVLDMQLAGRIIFVGSPPFLMNVCGCVSLAILNNQLQKFGGIRAIAV
                     MSVIFTVMSVVYMPLVGIAQGAQPIFGYNLGAARYDRVRSTLRLTLTVASILCMGVFI
                     FVETFPNLIFALFERDQSGLMRDGAYACRYFVMLLPLVGTIFVSTNYFQAINKPVESI
                     VLSLVRQSISVLSVLILPFWFGLDGIWISEPASTAIAFAVAVIMLLVEQKKLNNRQLE
                     LELPAKA"
     gene            4298..4999
                     /gene="rnc"
                     /locus_tag="PLK08_05315"
     CDS             4298..4999
                     /gene="rnc"
                     /locus_tag="PLK08_05315"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPS52953.1"
                     /translation="MISDEMLASCQELLGYKFKNTAILVQALTHTSAANTHRESNERM
                     EFLGDAVLGFSVCGILFRKYADLDEGDMTKIKSSVVSRSTCNEIVRQSGLGMFVIMDR
                     PIHSMQDLPDSIAACLIEAIIGAIYLDGGIRNAEKFIRANFQPYIDHAMSSAHQKNYK
                     SLLQQHVQRIGNNPPEYVVLDEQGPEHSKCFEVGIMIDGKHFASAWGKSKKLAEQAAA
                     RRALLNMGVIGEEDE"
     gene            complement(5412..>5832)
                     /locus_tag="PLK08_05320"
     CDS             complement(5412..>5832)
                     /locus_tag="PLK08_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52954.1"
                     /translation="DVKLNQSMGGTDMEASSKRPANYIPEDLSSQAMFELTKKKITTA
                     CFKSIYDAVPSRNVDIPFITLRCHYDGDENGLARLAVRSSLDNSSTEDSIYLFDSSGK
                     LVKIIYPNGITESLVDMQTLQRIFPNAMNVIKNISKE"
BASE COUNT         1399 a         1402 c         1541 g         1490 t
ORIGIN      
        1 ccgcatacgt accttccctg aagatgccga agacccagaa gtgacccttc tgacgcacag
       61 gcgggaagtg ctttcgcttg taatttacgg caataccagt ccgcaggtgc ttcacgaact
      121 ggctgaacgt accagagacg aactgctcca gcgaaaggat atcacgcagg tcgagatagt
      181 tggcgtcaag gctctggaga taaaaatcga gataccgcag gaaaatctcc ggcgttacaa
      241 tctgaccata gaccaggtgg cacagcggat aaaggctgcg gcggttgaaa ttccaggcgg
      301 cggcttgaag acgcgagcag gcgaaatact cgtccgcgtc aaggaacgaa aggattatgg
      361 cagacagttt gcggttctgc ccataatatc cacgccggac ggctctcagg tgaaactcga
      421 agacattgcc aaaattgacg acggcttcaa tgaagactcg gacacgcagg ctgtctacga
      481 caacaagccc gcggtcggca tagacgtcta cagcgtcggc gagcagaccc cgatgcagat
      541 ttcaaaatca gtcgccgagt tcaaggaaac tctcgatacg caactgccgc caggcattcg
      601 cagcgttatc ctggacgaca aaagcgtggt tttcaaacag cgcatagacc tgctggtttc
      661 aaacggcgtg tccggcctta ttctggtgat gataacgctc ggcctgtttc tggagattcg
      721 tcttgcgttc tgggtgatga tgggcattcc ggtttcattt atgggctcaa tgctcgtcat
      781 gccgctgctg ggcgttacca taaacatgat gtccctgttt gcctacatca tagccatcgg
      841 cattgtcgtc gatgacgcca tagtcgtagg cgaaaatatt tatcattacc ggcagaaggg
      901 caaaagcttt tacgaggcgt ccattctggg cgtcggcgac gtagccatgc ccgttacgtt
      961 cagcgtcctt acgaactgcg ttacgtttct gccgctgtac ttcatgcccg gcatgatggg
     1021 caaagttttc aacatgatac cgctggttgt catcacggtt ttcctgatat cgctgcttga
     1081 atccatattc gtgctgccgt cgcatcttgg ccatcagaaa gagtggaagc tgcgagggct
     1141 tttcggctgg atacatcatc gtcagcagga tttcagccac tggtttacga cgtggataaa
     1201 ttgtcgttac gggccattcc tggataaggc gattcattgg cgctatacta cgctgggcgt
     1261 aggcgtggcg atacttttgt gcaccatcgg ttatgccgta agcggacgaa tgggctttgc
     1321 actgtttccg aaagttgaat cggattacgc atatgccacg attactctgc cgtacggcgt
     1381 ggcattcatc aagacggaaa aaataagcga ccggcttttc aatgcggctc aaaaagtcat
     1441 aaaggaaagc ggccatgctg aacttgtcga aggtattttt acggtacttg aaacaaacag
     1501 cgtaaccatg aaaatcacca tggctcagcc tgagattcgc gaaaaaataa tgagtaccgc
     1561 cgaattcacg aaccgttggc gtgaaactac cggcacgctt ctgggcatag aaaacgccaa
     1621 gtttgaatcc gaccgcggcg gcccgggctc aggcgacgca cttaccatcg aactcagcca
     1681 ccgcgacatg aaagtgctgg aaaaagcaag tgccgaactt gccgccgatt tgagaaaata
     1741 tcctatggtc agcgatatag acgatggctt ttcacccggc aaggaacagc tggattttac
     1801 catcaagcct gaaggaaaaa gcctcggcct gacggcaaat gaaatcgcca ggcaggttcg
     1861 cagttcattc tacggcgccg aagccatccg gcagcagcga ggaagaaatg aaataaaagt
     1921 catggttcgt cttccaaaaa atgaacgctc cagtgaacat aatgtggaag acctcatgat
     1981 tcgtacgccg gacggcattg aagtgccact gcgggaagta gccaacttta cgaggggacg
     2041 ggcatatacg actatcgaca ggcgaaacgg aagaaccatc gtcaacgtca ctgccgacgt
     2101 gaccccgcaa agccgcgcag gcgaaatcat acgttccctc atccagaacg aactgcctgc
     2161 gctgatggct gagtatccag gtcttgcata cagctttgag ggcaaacagg ctgaccagcg
     2221 tgagagtctg ggaaatctgc aagtcggctt tctgctttca ttgctggtta tctatgcgat
     2281 gctggcaata ccgttccgca gctaccttca gccgcttatc gtcatggtca gcataccctt
     2341 tggcatcgtg ggcgcggtac tgggccacat aatcatgggt tatacgctca gcgtggtcag
     2401 cctgttgggc atagttgcgc tagccggcgt agtcatcaac gattcactgg tgttaatcga
     2461 gctggccaac aaacttcgca aggacaacgc cctccgcaat cccgcggagg cgattcacgc
     2521 cgcagccatt ggccgttttc gcgcgattat acttacgacg cttaccacgt ttttcggctt
     2581 ggctccgatt atctgcgagc agtcgcgtca ggcgagattc cttattccca tggcgatttc
     2641 acttggattc ggcatattgt ttgccacgtt cataacgtta atattagtgc cggcgttata
     2701 cgttatgatt gaagatatga aaaatattta cgccggcttt tttaaacgca agaagagcag
     2761 taatgtcgct tgagcaggct cacagactgg aacatgaaaa aattccgcag ttgttgttac
     2821 ggttttccat accttcgacg gttgcgacgg ttgttcacgg cacatacaac gtcgtggacc
     2881 gtatttttgt cgggaaagcg gttggcgaag aggcgctcgc cgcgctcgcc gtcgccatgc
     2941 cggtcatgat gataatgatg gcgttcggca tgatgtttgg ccgcggcggc agtgcgctga
     3001 tttccattca tatgggtgaa aaacgttttg acaaggctga aagggttttg ggaacaggcc
     3061 ttttactgtt ttgcatattg tcttttctgt ttacgtgctt cggcctgata tacatagacc
     3121 cgttgctgcg catattcggc gcaaatgatg ccataatgcc catggctcgt gaatatcttt
     3181 cgataatact ctggggttcg ctgtttcatg aaatatcttt cggcgtgaac aactatatcc
     3241 gttccgaggg caacgccaaa acagccatga tgaccgcgtt ttttgctgcc ggcctcaatg
     3301 tggttctcga cccgatattc ctgttcgtgt tcaatatggg cgtagccggc gtggcatggg
     3361 cgaccataat cgcacagttt gcctccgccg tatgggtaat gctctattat ttcggcggca
     3421 aaagtctgct gagattccat ctgaaaaatc ttgtgctgga tatgcaactc gccggccgga
     3481 taatttttgt cggctcgccg ccgtttttaa tgaacgtctg cgggtgcgta tcgctggcga
     3541 tattgaataa ccagctccag aaattcggcg gcattcgcgc tattgccgta atgagcgtca
     3601 tattcacagt gatgtcggtg gtatatatgc cgctggtcgg catcgcccag ggtgcgcagc
     3661 ccatattcgg gtataacctc ggtgcggcaa ggtatgacag ggtacgttca acgttgcggc
     3721 tgacgcttac tgttgcaagt atcctttgca tgggagtttt catattcgtg gaaactttcc
     3781 cgaatcttat tttcgcactg tttgaacgcg accagagcgg cctgatgcgg gacggcgcgt
     3841 atgcctgccg ctatttcgta atgcttctgc cgctggttgg cacaatattc gtatcgacga
     3901 actattttca ggcgataaac aaacccgttg aatcgatagt tctttcgctg gtgcggcaga
     3961 gcatttccgt gctttcagtt ctgattctgc cgttctggtt cggcctcgac ggcatatgga
     4021 tatccgagcc ggcgtctacg gctatcgcgt ttgccgttgc cgtaatcatg ctgcttgtcg
     4081 agcagaaaaa actcaacaac cgtcagcttg aacttgagtt gccggctaag gcctgataga
     4141 attgcttaca tgtaaaggtc cagcgtgcaa gtttacgtcg tgcgttgcgg cggaagcttt
     4201 cacgccggac cttattggaa aaatcaggcc gatatcggac gacgatattt ctgttctgcg
     4261 gacgaaaagc cggcttgaag aaaaaggtct ttcaacaatg atatcagatg aaatgctggc
     4321 tagctgccag gaattgttgg gttacaaatt caaaaacacg gcgattcttg tgcaggctct
     4381 tacgcatacg tcagccgcaa atacgcatcg tgaaagcaat gaacggatgg aatttctggg
     4441 cgatgccgtt cttggttttt ccgtatgcgg cattctgttc cgcaagtacg ccgacctcga
     4501 tgagggcgac atgaccaaaa tcaagtcttc ggtcgtatca cgcagtacgt gtaatgaaat
     4561 tgtccggcag agcggtttgg gcatgttcgt catcatggac cggcctatac actcaatgca
     4621 ggatttgccg gattccatcg ccgcctgtct gatagaggcc attatcggcg ccatttatct
     4681 tgacggcggc atcagaaatg cggaaaaatt catacgtgca aattttcagc cgtatattga
     4741 ccatgcgatg tcaagcgcac accagaaaaa ctacaaatcg cttttacagc agcacgttca
     4801 acggattggc aacaatccgc cggaatatgt cgtactcgac gaacaggggc ctgaacacag
     4861 caaatgcttc gaggtcggaa tcatgattga cggcaaacat ttcgcaagcg cttggggcaa
     4921 gagcaaaaaa ctcgccgagc aggctgccgc caggcgtgcc ctccttaata tgggtgtaat
     4981 cggcgaagag gatgaatgat gccggataga ttgtgagccg ttagagttgt ttccgaaatg
     5041 gtttttttga aaatcatcta aaaaaaatca atatagcgcc aaaaagtatc ttgaaaaaag
     5101 ttaaaactca cggcattatt gtgccgatat ttgttaatgg aatagtgttg tttggcatat
     5161 ggaggtgcct gaaatgacgt tgaagacatt gagaatgaag gcttcgccgg ttgtatggga
     5221 attggcattg atggcggtgt ggctgctggt tgcgctgctt atcatcatga aacagtgacc
     5281 ggttttgaaa cgatatggat ttatcctttg cctgcgggtt gtcttgccgg ttttgcggta
     5341 tttgcatacc gccagtcgta agccggaata acgccttttc agatgaaagc ggtacggttt
     5401 ccagtttcgg attactcttt cgagatattc tttatcacat tcattgcgtt ggggaaaatt
     5461 ctttgcagcg tctgcatgtc gaccagtgat tccgttatgc cgttcggata gataattttg
     5521 acgagtttgc cggacgagtc gaagagatat atcgaatctt cggtggaaga gttgtccagg
     5581 ctgctgcgca ctgccagcct tgcaaggccg ttttcatcgc cgtcgtaatg acaccgcaga
     5641 gttatgaacg gaatgtcgac gtttcgcgac ggcactgcgt cgtagatgga tttgaaacac
     5701 gccgtggtga ttttcttttt tgtcaattcg aacatagcct ggctgctgag gtcttcaggg
     5761 atataattcg ccggcctttt tgacgaggct tccatgtctg ttccgcccat gctctgattc
     5821 agtttgacgt cg
//
LOCUS       DAONFI010000128         5813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16171_length_5813_cov_4.707885, whole genome shotgun sequence.
ACCESSION   DAONFI010000128 DAONFI010000000
VERSION     DAONFI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16171_length_5813_cov_4.707885"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1273
                     /locus_tag="PLK08_05325"
     CDS             182..1273
                     /locus_tag="PLK08_05325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPS52955.1"
                     /translation="MNRFLVALSSVAVVFVGMVFAQSDSKPAATDKPVIAQLPAPEAV
                     KLPPLPEVVATVGDIKIPGARIEKMISKLPATVPASVQVNLRQRALSALLFNALIENY
                     VAKHDIKPSDADLKDINARLEEIAKQRNMTVDEVMKTEGITKKELETQAAAQHIFNDV
                     TSKEKVEALVKAHPEYFNGTEVQASHILVLCAPMAQTKDAMAALDKIKKIRADILAGK
                     IKFDDAANKFSECPSGKRKGQDGKTEYGDLGYFAFSKDGKMMMAPTFAVAAFATKVRE
                     VSDVIRTQFGFHIIKVLNRKDGTAKYEAAQADTVAKVVLESEFQMSIFDQIINGCDVT
                     IDLDAFKMPEEAEPAATAAAPAPAPAAKK"
     gene            1492..2151
                     /locus_tag="PLK08_05330"
     CDS             1492..2151
                     /locus_tag="PLK08_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPS52956.1"
                     /translation="MTGRYRKNSDGIFDISAGRMTADRWRVRERILKIITFLLILAAI
                     GGVIALDRAGIFGTASCGDFEKYDNKKFCVMRVIDGDTLDVDCIDGEYLLTRIRLWGV
                     DTPETVKPNCPVEHFGMEASGFTRRNTLNKMVRLELVQTSTRDKYGRLLAYVYLPDGR
                     MLNRELVAAGYAYADPRFQHPRKSEFIRLADDARKNGAGLWSQPHKADWPYYVKGSLR
                     K"
     gene            2497..2691
                     /locus_tag="PLK08_05335"
     CDS             2497..2691
                     /locus_tag="PLK08_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52957.1"
                     /translation="MRKSKENQAEKQPTRIAGIRCIDTRALSRIISWSIGSCEQQLMT
                     LKSAMNILAEEIRASQKKAR"
     gene            complement(2704..5259)
                     /locus_tag="PLK08_05340"
     CDS             complement(2704..5259)
                     /locus_tag="PLK08_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52958.1"
                     /translation="MDHFTLEKIDFPAIRRLLAEFCNCSLGRELAMRMCPAARIELVR
                     QWLDETSQMVEILRDSGVVPFGGVTDIRPSLDRAQPGRGATADDFSNIAATLEAALNI
                     KNFLSAQPESFFRIHSLAANIGDFSTQTKLIRHVIGPDGAVRDDASPRLAQLRNQISS
                     AAMRIHEIIENYLHNADVRRLLQSPTITLHGDRYVLPVKAENRGRLSGVVHRASHTGQ
                     TVFIEPVESVELNNTLVDLHEHERQEIERLFSELAIRLVARAAEIERTVRTLAKIDLI
                     SAKAQYTYQYEMTRPEIGADSLEFFQARHPLIEAADVKHRKLFPDQPTKPVVPIDIRL
                     GSDFDVLLITGSNTGGKTVSLKTVALLVAMAQSGLHIPAAPGAKMPIFEDVLIDIGDE
                     QSLEQSLSTFGAHIERMKYILHRLNRLGRQCLVLLDELGSGTDPDEGGAIGQAVIDEL
                     RRKNCMAVITTHLSILKAYALNHDRVENASVEFDTVSLQPTYKLRIGTPGESHAITVA
                     QKLGLPGRIIHDAKRHFAGSGRQLRKALRMTGEARLQAEEARAKATQAAIDAKSQIDE
                     YESHLEDIKKLRADMAEWMAGLSEMQPGEMLHVPSLNRAGRLVRMELHRQRALVDVDN
                     MQVEVPLLDLMPDLGQQGIRLQLQQHRRQMRELLAQAQAMRTEAEDFREESRKELNRR
                     RRHVQEFDRWVELIAGVKTGDDVPISRKPGHGRVVKIDFAAAKAIVELPDKSQAELPL
                     RDIFPQSGPYAPDKKKGRTTAAASENDEPVPHGRAVGKKAANTRDRILQTNAGEKVFV
                     VPFNTTATLVRVDPTRDKAVVLKGAFEIEVAITDIEPAAIKTLSPQTDKANQQ"
     gene            complement(5265..5729)
                     /locus_tag="PLK08_05345"
     CDS             complement(5265..5729)
                     /locus_tag="PLK08_05345"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPS52959.1"
                     /translation="MADDAPKIIVDDDWKNQAQAEKAKLEEEARKEQEERQAHQLPEA
                     SFVTLVNQIAMQAVMALGGVEEPSSGKRILDLGLAKFQIDTLAVLKEKTKGNLNDEET
                     KFIDTTINELQMAFVSMAKQAQAMMQQKAANAGAAAPAAADAASAAKPKIEI"
BASE COUNT         1279 a         1448 c         1629 g         1457 t
ORIGIN      
        1 attttttctt tgcttacttt aaaaaaattt gaacaattag aaattctggt tcgtatatac
       61 atttaaaatg tcgtgcctta cgggaaaagt agcgagccga cttttgggtt ttgttttaaa
      121 aaagttaaaa aagttaaaaa gttaaaaaat ttagacaaac agaatttgaa ggagttaaaa
      181 aatgaacaga tttctagttg cgctgtcgag tgtggcagtt gtattcgtgg gaatggtttt
      241 tgcacaatcc gacagcaagc cggcggcgac ggataaacca gtaatcgccc agttgccggc
      301 gccggaagcc gtcaaacttc cgcctctgcc ggaagtggtc gccaccgtgg gcgatatcaa
      361 gattcctggc gccagaattg aaaaaatgat ttccaagctg ccggcgaccg ttcccgcgtc
      421 ggtgcaggtt aacctgcgtc agagagcgct gagcgcactg ctttttaacg cacttatcga
      481 aaactacgtt gcaaaacatg acatcaaacc cagcgacgcc gatttgaagg acatcaatgc
      541 ccgcctggag gaaatcgcca agcaacggaa tatgacggtt gacgaagtca tgaagaccga
      601 aggcataacc aagaaagaac ttgagacgca ggctgccgcc cagcacattt tcaacgatgt
      661 tacgtcgaag gaaaaagtgg aagctctcgt caaggcgcat ccggaatatt ttaatggcac
      721 ggaagtgcag gccagccata ttcttgtgct ttgtgcgcct atggctcaga ccaaagatgc
      781 gatggcggct ctggacaaaa tcaaaaagat tcgagccgac atcctcgccg gcaagattaa
      841 attcgacgat gctgccaaca aattttcgga atgtccgtcg ggcaagcgta aaggccagga
      901 cggcaagacc gaatatggcg atttgggcta ttttgctttc agcaaggacg gcaaaatgat
      961 gatggcgccg acatttgctg tagcggcttt tgcgacgaaa gtccgcgaag tcagcgatgt
     1021 catccgcacg cagttcggtt ttcatatcat caaggttctc aaccgtaagg acggtactgc
     1081 gaaatatgaa gctgcccagg ctgatacggt tgcgaaagtt gtgctggaaa gcgaatttca
     1141 gatgagcatt ttcgaccaga ttatcaatgg ctgcgatgtg acgatagacc tggacgcatt
     1201 caagatgccg gaggaagctg aaccggcggc taccgcagcg gcccctgccc ctgctccggc
     1261 agccaaaaaa taaggtttgc ttcaattttt taaatgaaaa tagaaagatt ctatggcttt
     1321 aaaaccgtag agttacgaac ggcatatcaa aaaaatcccg cccgcctttt gaagacggac
     1381 gggatttttt aatatgtcat atcattttac gaaaaaattt acttttgggc tagagcatct
     1441 taacttgagt atgctctaat ccgtaaagat tcactattgg cacgtaaaaa aatgacaggg
     1501 cgttacagaa aaaactcgga cggcattttt gacataagcg caggccgcat gacggcggat
     1561 cgctggcgag tgcgcgaacg aattttgaaa attatcacat ttctgttgat actggcagcc
     1621 atcgggggcg taatcgcgtt ggacagggcc ggaattttcg gcactgccag ttgcggggat
     1681 tttgaaaaat acgacaataa aaaattttgc gttatgcggg taatagacgg cgatacgctg
     1741 gacgttgact gtatcgacgg cgaatatctt ttgacgcgaa tccgtttgtg gggcgtcgat
     1801 acgcctgaga ccgtcaagcc gaactgtccc gtcgaacatt tcggcatgga ggcgagcgga
     1861 tttacgcggc gaaacacgtt gaataaaatg gtgcgtctcg aactggttca gaccagcacg
     1921 cgggacaagt atggcagatt gctggcgtat gtatatctgc cggacggcag gatgctcaac
     1981 cgcgagttgg tagccgccgg ttacgcctac gccgacccgc ggtttcagca tccgcgcaag
     2041 tcggagttta tcaggttagc cgatgacgct cgcaaaaacg gcgcaggctt gtggtcgcaa
     2101 ccgcataagg ctgactggcc gtactacgtc aagggaagtc tgcgaaaata aagcgacgat
     2161 acggaatcgc aaaaacatat ctgccatgcc gccgtcagac gaagttgttt catcccataa
     2221 attcacttgt tttgctccac gtttttacat aacattttca gtgtaataca acaattaaat
     2281 atggcggggg tttggacgtt ccaacacccc tgattagccg ttaatctgtg cccacgaaac
     2341 cgcaacattt ctaaaagagt accgaacggc atatgacagg ccaatgtcct tgtgacattg
     2401 ggtctcgtgc gtatgtgtgt tcggtaggcc tgttgcgggc cttgtgggcc acatccgcga
     2461 ggcccttttt tttagggtct gcaaggggac gtgataatgc gtaaaagcaa agaaaatcag
     2521 gcagagaagc agcccactcg aatcgccggc ataagatgca tcgacactcg cgcactgagc
     2581 aggataattt catggtccat cggcagttgt gaacagcagt tgatgacatt gaagtcagcg
     2641 atgaacatcc ttgccgaaga aatcagagcg tcgcagaaaa aagcccgata gagctgaagg
     2701 ctgttattgc tggtttgctt tgtccgtctg gggtgagagt gtttttatag cggcgggttc
     2761 gatatcggtt attgcgactt ctatttcgaa tgcccctttc agcacgacag ccttgtcgcg
     2821 ggtcgggtcg actcttacga gtgttgccgt tgtgttgaac ggtacgacga agactttttc
     2881 gcccgcgttt gtttgaagga ttctgtcgcg ggtgtttgcc gcctttttgc cgacggctct
     2941 gccgtgcggc actggttcgt cattttccga tgccgcggca gtcgtgcggc cttttttctt
     3001 gtccggcgca taaggccccg attgcgggaa tatgtcgcgc aacggaagtt ccgcctgcga
     3061 tttgtcgggc agttcgacaa tggcttttgc ggcggcgaaa tcgattttta cgactctgcc
     3121 gtggcccggc ttgcgtgaga ttggtacgtc gtcgccggtt ttgacgccgg ctatcagttc
     3181 cacccagcgg tcgaattcct gtacgtgccg ccggcggcga ttgagttctt tgcgggattc
     3241 ttcgcgaaaa tcttccgcct ccgtccgcat tgcctgtgcc tgtgcgagca gttctcgcat
     3301 ttggcggcga tgctgctgta attgcagcct gatgccctgc tggccgaggt cgggcatgag
     3361 gtcgagcagc ggcacttcaa cctgcatgtt gtccacgtcc actagtgctc gctggcgatg
     3421 cagctccatg cggacgagtc tgccggcgcg gttcagcgat ggcacgtgca acatttcacc
     3481 aggctgcatc tcggaaaggc cggccatcca ttccgccata tcggcacgga gttttttgat
     3541 gtcttcgagg tgcgattcat attcgtcaat ttgggatttc gcatcaatgg cggcttgcgt
     3601 agccttcgcg cgagcctctt ccgcctgaag cctggcttcg ccggtcatgc gaagcgcctt
     3661 gcgaagctgt ctgccgctgc cggcgaaatg ccgtttcgcg tcgtgaatta tcctgccggg
     3721 caggccgagt ttttgtgcga cggttatggc gtgactttcg cctggcgtgc cgatgcggag
     3781 cttatatgtc ggctgaagcg aaacggtatc gaattcgacg gacgcgtttt caacgcggtc
     3841 gtgattcagg gcgtaagcct tcaggatgga caggtgcgtg gttatcacgg ccatgcagtt
     3901 tttgcggcgg agttcgtcta ttaccgcctg gccgatggcg ccgccctcgt cggggtctgt
     3961 cccgctgccg agttcatcca gcagaacaag acactgcctg cccagccggt tcagccggtg
     4021 cagaatgtat ttcatccgct cgatgtgcgc gccgaatgtg ctgagcgact gttcgagcga
     4081 ttgctcgtcg ccgatgtcga tgagtacgtc ttcgaatatc ggcatttttg cgcccggcgc
     4141 agccggtatg tgcagcccgc tctgtgccat ggcgaccagc agtgcgacgg ttttgagcga
     4201 aaccgttttg ccgccggtgt tgctgccggt tatcagcagc acgtcgaaat cgctgcccag
     4261 gcggatgtct atgggcacga cgggttttgt cggctggtcg gggaagagtt tgcggtgttt
     4321 cacgtcggcg gcttctatga gagggtgacg agcctggaaa aattcaaggc tgtcagcgcc
     4381 aatctccggc cgtgtcattt cgtactgata cgtgtattgc gccttggcgg aaatgaggtc
     4441 gatttttgcc aatgtccgga cggttcgctc aatttctgcg gctctggcga cgaggcggat
     4501 agccagctcg ctaaaaagcc tctctatctc ctgtcgctca tgctcatgga ggtccaccag
     4561 agtattgttg agttcgaccg attcaacggg ttcgataaaa acggtctggc cggtatggct
     4621 ggctcgatga acgacgccgg aaagacggcc tctgttttcg gctttgacgg gcaggacgta
     4681 acggtcgccg tgcagcgtga tggtgggact ttgaagcagc cggcgaacgt ccgcgttgtg
     4741 aagataattt tcgatgattt catgaatccg catggcggcg gacgatatct ggttgcggag
     4801 ctgcgccagc cgcggcgatg cgtcgtcacg gacggccccg tcagggccta tgacgtggcg
     4861 gatgagttta gtctgcgtgg agaagtcgcc gatattggcg gctaacgagt gtatgcgaaa
     4921 aaaactctcc ggctgtgccg acaggaaatt cttgatgttc agcgcggctt ccagggttgc
     4981 tgcgatattg ctgaagtcgt ctgccgtagc acctctgccc ggctgtgccc tgtcgagcga
     5041 cggccgaata tccgtcacgc cgccgaatgg aacaaccccc gaatcgcgca gtatttcaac
     5101 catctgcgaa gtttcgtcca gccattgtcg cacgagttca attctggcgg ctggacacat
     5161 ccgcatcgcc aactcacgcc cgagactgca attgcaaaac tccgcaagca gccgccggat
     5221 ggcgggaaaa tctatcttct ccagcgtgaa atggtccata tgcgttatat ttcaattttg
     5281 ggcttggcgg ctgaagcggc gtcggctgct gccggtgcgg ctgcgcctgc attggcagcc
     5341 ttctgctgca tcatcgcctg agcctgcttg gccatcgata caaacgccat ctgcagctcg
     5401 ttgattgtag tatctatgaa cttcgtttct tcgtcgttca ggttgccctt ggttttttcc
     5461 ttgagtaccg cgagcgtgtc tatctggaat ttggcaaggc cgaggtcgag aatccgtttg
     5521 cctgaggagg gttcttccac gccgccaagc gccatgacag cctgcatggc tatctggttg
     5581 acgagggtca caaagctggc ttccggaagc tgatgagcct gccgctcttc ctgctctttg
     5641 cgggcttcct cctccagctt agccttttct gcctgtgcct gatttttcca gtcgtcatca
     5701 actatgattt tcggggcgtc gtcagccatg gtaattcctt tcttctgcgt tttttaattt
     5761 tttaatatgt ccttcgtaat tctacggttt ttaaaccgta gatgaaagta tcc
//
LOCUS       DAONFI010000129         5807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16200_length_5807_cov_4.212969, whole genome shotgun sequence.
ACCESSION   DAONFI010000129 DAONFI010000000
VERSION     DAONFI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16200_length_5807_cov_4.212969"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLK08_05350"
     CDS             complement(<1..1009)
                     /locus_tag="PLK08_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52960.1"
                     /translation="MKNIMALSILAASLAFVGCNSRPAVPHSKWYSPQPVKSIDVNPS
                     DKTEVQAVTKYANARRLYYRALSALVDYYKSIGDVQKGKWADTEMKNLKKTQEFTWRN
                     LDVPEEKFSPAAAEPNEQLLVENVVDSREKYVQAMDDLAKYYEKSNQTLKAYVVHVTQ
                     ERFRPEETFYYISKATLPPLDLLPCKVIPEANSLYDEALKLYREGALLPAMADYKKER
                     RAIELFTRLITDYPQSTRIAMSAYYIAEIYKEYFREHYLAVQWYQRAMAWDPHLAAPV
                     RFQCAVQYDFNLGEPGKALTLYKAAMQLEPYYPSHMDFCKDRIKELEKKYAPKSLDDT
                     ER"
     gene            1395..1649
                     /gene="rpsP"
                     /locus_tag="PLK08_05355"
     CDS             1395..1649
                     /gene="rpsP"
                     /locus_tag="PLK08_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPS52961.1"
                     /translation="MAVKLRLKRMGRTHKSFFRLSAIDSRSPRNGRVIEELGWYDPNA
                     DTIEEKTSLKRERIEYWLSVGAQPSETVRDLLKSRGIAVK"
     gene            1697..2413
                     /gene="trmD"
                     /locus_tag="PLK08_05360"
     CDS             1697..2413
                     /gene="trmD"
                     /locus_tag="PLK08_05360"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPS52962.1"
                     /translation="MAMRIDILTLFPEFFDAFRNTSIPGRAVRNGFVDISTTSIRDFA
                     IDRYGSVDDAPFGGGPGMVLRCEPVFAAVEHVRKNCPEPAGKVILMTPQGRPLKQNIV
                     RELAAEPRLILIAGHYEGFDERIRTTLADDEISLGDFVLSGGEIAAMALADAVIRLLP
                     GALGKDASSEEESFSGGLLEYPHYTRPREFMGQAVPDVLLSGDHARIEQWRLEQSQLR
                     TRIKRPDLWEQYLRQTQDKN"
     assembly_gap    2552..2561
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2592..2942
                     /gene="rplS"
                     /locus_tag="PLK08_05365"
     CDS             2592..2942
                     /gene="rplS"
                     /locus_tag="PLK08_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPS52963.1"
                     /translation="MSQELLKKAAQSSMKSEVPSFKVGDTVKVHCRIVEGDKERIQMF
                     IGTVIGRSGGGIGETFTVRRIVNNEGVERTFPVHSPKVADVEVVRSGRVRRAKLYYLR
                     DRVGKATRLKELRD"
     gene            2953..3369
                     /locus_tag="PLK08_05370"
     CDS             2953..3369
                     /locus_tag="PLK08_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPS52964.1"
                     /translation="MWWPFNRLISKKNATPTSGECDNISGPRGEKIACRFLRRAGFRI
                     LARNYRCPTGEADIIAVDAVADAIVFVEVKTRKSNAAVEPYSAVNADKKRRMKKIAAY
                     YAQKHPVEDYTIRFDIISIVLPSDGQPKIEHIYDAF"
     gene            3356..4165
                     /locus_tag="PLK08_05375"
     CDS             3356..4165
                     /locus_tag="PLK08_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPS52965.1"
                     /translation="MTLSEKFENSGESLPTLIDTHCHFAHELLNENIPRVLDDARISG
                     VRRIIAAAGNIEESRFNCELAKNFEQISALVGIHPHDSKDAPADLAGEFAEFVGSCVG
                     IGEIGLDYHYDLSPRDVQREVFARQLEVAKNLNLPVAIHSREAFDETMAILRESAIEP
                     QMVLFHSFAQGQADATTALDYGAMLSFSGMATFKNASDILAAARMTPSERIMVETDSP
                     YLSPVPVRHIKPNVPAHVYHVAVHLAAARGETFSQFAAATTKNATAFFGLK"
     gene            4273..4911
                     /locus_tag="PLK08_05380"
     CDS             4273..4911
                     /locus_tag="PLK08_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPS52966.1"
                     /translation="MKWTLPNQITVARMILSIVFFVLLACYDPQMHNGALLLKVSFVV
                     FLFAALSDVLDGYLARKLNQVSAFGRIVDPIMDKVMVCGGFIFLMSPVYREVISSYEQ
                     TLPHWLTGQTASNIQPWMLVVIIGREFLISGIRGYSESIGKQFPATWSGKLKMFLQCF
                     ALGTVLFVMAWTPNVAWTIWVKIVAVWLAVLVTLYSGIIYIVNARGLFKEDR"
     gene            4950..5471
                     /locus_tag="PLK08_05385"
     CDS             4950..5471
                     /locus_tag="PLK08_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPS52967.1"
                     /translation="MNFFRKFIISGMGMGYLRPAPGTWGSAAAAGVYLACRYFAGPNM
                     VLLTLIMLAVAILSTTGCVMLGDFTERWYGRKDPSHCTLDEWAGQSIALLFLPCEYSW
                     KSTLIFVAAAFVAFRVFDILKPAPARNFERFDKGIGVVADDLVAGLYACVTVQLLIKS
                     AVFLPAVNYWNFS"
     gene            5570..5807
                     /locus_tag="PLK08_05390"
                     /pseudo
     CDS             5570..5807
                     /locus_tag="PLK08_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981537.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
BASE COUNT         1351 a         1415 c         1573 g         1458 t
ORIGIN      
        1 gcctttcggt atcgtccagc gactttggag cgtatttctt ttccagttcc tttatacggt
       61 ctttgcagaa atccatgtgg cttggatagt agggctcaag ctgcatggct gctttataga
      121 gcgtcaacgc cttgccgggt tcgccgaggt tgaaatcata ttgcactgcg cactggaatc
      181 gtaccggcgc agccaggtgc gggtcccacg ccatcgcacg ctgataccac tgcactgcaa
      241 gataatgctc gcggaagtat tccttatata tttcggcgat gtagtatgcc gacatcgcga
      301 tgcgggtgct ctgcggataa tcggtaatca gccgcgtaaa aagctcgatg gcgcgacgct
      361 ctttcttgta gtcggccatc gcgggcaaga gtgccccttc acgataaagt ttgagagcct
      421 cgtcgtaaag gctgttcgcc tcgggtatga ccttgcaggg aagaaggtcg agcggcggca
      481 aggtagcttt gcttatatag taaaaagtct cttccggcct aaaacgctcc tgcgtgacgt
      541 gaaccacgta agcctttagc gtctgatttg atttttcata atatttcgcc aggtcgtcca
      601 ttgcctgtac gtatttttca cgcgaatcca cgacgttttc caccagcagc tgttcgttgg
      661 gttcggcggc tgcgggcgaa aatttctctt ccggcacgtc caggtttctc catgtgaact
      721 cctgggtctt cttgagattt ttcatttccg tgtcagccca tttgcccttc tgcacgtcgc
      781 cgatggattt gtaataatcc acaagagctg acagggcacg gtaatacagc cgtcgtgcat
      841 tggcatattt cgtgaccgcc tgaacttctg tcttgtccga cgggttgacg tctatacttt
      901 ttaccggctg tggagaatac cattttgaat gcggcaccgc cggcctgctg ttgcacccca
      961 caaaagcaag cgacgccgca agaattgaca aagccatgat gtttttcatt attctcactc
     1021 ctgcaagaag aaatgacgat ttttctttaa ggggatatta ttgcatcaag cggcctttct
     1081 tgcaacacct gcgatggaaa aaatcccgaa agtctgtcaa ataattaatt cgtataagat
     1141 gacgaacgtc ggctgacgtt caggcgtgta ttggttatgt tcggcatttt gtttgtcatt
     1201 ctgacgacgg tttttgagcc tgccggttta aaaatcttga aaaatcatta aaaagggctt
     1261 gaagttcggg gcttggcaag taatatattt cgttcctcgg tttgtagtcg gagtgtatct
     1321 gcaaaccacg gctgaaatgc gctttcagcc ggaacgttaa tgaatgatga tacttacagt
     1381 atcggagata tcaagtggca gtaaaattga gactcaaaag aatgggtcgc actcataaga
     1441 gtttctttcg gctgagtgcg atagattcgc gtagcccgcg taatggccgc gttattgagg
     1501 aactcggctg gtatgatccc aacgccgaca ccatcgagga aaagacctcg ctcaaacgcg
     1561 aacgcattga gtattggctt agcgtaggcg ctcagccgtc cgagacggtg cgcgacctgc
     1621 tgaagtccag aggcattgcc gtcaagtgag acggtgcatt ccgtcgggat gctgatggcc
     1681 tgcggatagc cagcctatgg cgatgcgaat agacatattg acgctgtttc ctgagttttt
     1741 cgacgctttc aggaacacca gcattccggg ccgggcagtt cgcaatggct ttgtggatat
     1801 aagtactacg agtatcaggg atttcgcgat tgaccgctat gggtcggttg atgatgcccc
     1861 gttcggcggc gggccgggaa tggtgctccg gtgcgagccg gtttttgcag ccgtcgaaca
     1921 tgttcgtaaa aattgccccg aacctgccgg caaagtcata ctgatgacgc cgcaaggcag
     1981 gcctttgaaa cagaatattg ttcgtgaatt agccgccgag ccgagattga tacttatcgc
     2041 cggccattat gaaggttttg acgaacgaat cagaaccacg ctggctgacg atgagatttc
     2101 gctgggcgat tttgtactct ccggcggaga aattgcggcg atggcgttag ccgatgccgt
     2161 catacggctg ctgcctggcg cgttaggcaa agacgcctcc agcgaagagg aatctttcag
     2221 cggcggcttg cttgagtatc cgcactatac gcggcccagg gagtttatgg gtcaggcagt
     2281 gccggatgtg ctgctcagcg gcgaccatgc caggattgag cagtggcggc tggaacagtc
     2341 gcaactgcgg acccgcataa agcggcccga cctctgggaa caatacctgc ggcagacgca
     2401 ggataaaaac tgaatataaa aatttattaa aagtgatttt acggattaat acccgtagca
     2461 ttgtgtaatt atcacaggct ttcgcctgtc cgcctgatgg cggatgtttt cgtctgcgtt
     2521 ttttagtttt tttaaaaaaa acgtagaatt annnnnnnnn ntaaaaaaat aaaaaacttg
     2581 gagataataa aatgtcgcag gaattgctga aaaaagcggc tcagtcgagc atgaagagtg
     2641 aagtaccgtc cttcaaggtc ggcgataccg tcaaggtgca ctgccgcatc gtcgaaggcg
     2701 acaaggaacg tatacagatg ttcatcggta cggtcatcgg ccgttccggc ggcggcatcg
     2761 gcgagacctt taccgttcgt cgcatcgtca ataacgaagg cgttgaacgt actttccccg
     2821 tccacagccc gaaagtcgcc gacgttgaag tcgtgcgtag cggcagggtt cgcagggcaa
     2881 agctctacta tctgcgcgac cgcgtaggca aggctacacg cctcaaagaa cttcgcgact
     2941 gaggagcagg agatgtggtg gccgtttaac cggctgattt cgaaaaaaaa cgctacgccg
     3001 acgtccggcg agtgtgacaa catatcaggc ccgcgcggcg aaaaaatcgc atgtcgattc
     3061 cttcgtcgcg cgggctttcg tattctagcg cgaaattaca gatgcccaac tggcgaggct
     3121 gacataatag cggtcgacgc cgtagccgat gcgatagtct tcgtggaagt aaaaaccaga
     3181 aaatcaaatg ccgccgtcga accatactcc gccgtcaacg ccgacaaaaa acgacgaatg
     3241 aaaaaaatcg cagcctatta cgcgcaaaaa caccctgtcg aagattacac gatacgattt
     3301 gatataattt cgatagtcct gccgtccgac ggccagccga aaatagagca tatatatgac
     3361 gctttctgaa aaatttgaaa attcaggcga atccttgccg acgcttatcg acacgcactg
     3421 tcactttgca cacgaactgc tgaacgagaa cattccgcgt gtgcttgacg acgcacgaat
     3481 atccggcgtg cggcggataa ttgcagcagc cggcaatatc gaagaatcgc ggttcaactg
     3541 tgaactggcg aaaaattttg aacagataag cgcgcttgtg ggaattcatc cgcatgactc
     3601 gaaagatgcg ccggctgacc tggcagggga gtttgcggaa tttgtcggca gttgcgtcgg
     3661 catcggcgaa atcggcctgg attatcacta cgatttatcg cctcgcgatg tgcagcggga
     3721 agttttcgcc cgccagcttg aagttgcaaa aaacctcaac ctgccggtgg ccattcattc
     3781 gcgggaagcg ttcgatgaga cgatggcaat tctgcgtgaa tcggctatcg agccgcaaat
     3841 ggtgctgttt cattctttcg cacaggggca ggctgacgct acgactgctc tcgattacgg
     3901 ggcgatgctg agtttttccg gcatggccac cttcaaaaac gcgtctgaca tattggcggc
     3961 tgcgagaatg acgccgtccg aaagaattat ggtggaaacc gactcgccat atctatcgcc
     4021 cgtgccggtg aggcacatca aaccgaacgt gccggctcac gtctaccatg tagccgttca
     4081 cctcgcagcc gcccgcggcg aaactttttc acaattcgcc gccgccacca ccaaaaacgc
     4141 gaccgcattt ttcggattga aatgacgcga gttgcccgtg attctgaaat tgcagataac
     4201 cagcaggatt tttcaacaag tatgttggca ttcgcgcgac ggttggttac aatccattcg
     4261 taggtgaaaa atatgaaatg gactctgcca aatcagataa cggtggcacg gatgatactc
     4321 tcgatagttt tcttcgtgct tcttgcgtgt tatgacccgc agatgcataa cggtgcgctg
     4381 ctgctcaagg tgagctttgt tgtattcctg tttgcagcgc tcagcgacgt tctggacggg
     4441 tatcttgcac gcaaactcaa tcaggtttca gctttcggcc gcatagtgga cccgataatg
     4501 gataaggtca tggtatgcgg cgggttcatt ttcctgatgt cgccggtata tcgcgaagtc
     4561 atctcgtcgt atgagcagac gcttccgcac tggctgaccg gccagacggc aagcaacatt
     4621 cagccgtgga tgctggtggt catcatcggc agggaattcc tcatttcagg cattcgcgga
     4681 tatagcgaat ccatcggcaa acagtttccg gctacatggt cgggcaagct gaaaatgttt
     4741 ctgcagtgtt tcgcactggg aacggtactc tttgtgatgg cttggacgcc gaacgtcgcg
     4801 tggacgatat gggtaaaaat tgtggcggtg tggctggcgg tgttggtgac gctttacagt
     4861 ggaatcatat acatcgtcaa cgcgagaggc ctttttaaag aagacagatg aatccgtctg
     4921 atttcttccg atttttttaa agagaataaa tgaatttttt caggaaattt attatcagcg
     4981 gcatgggcat gggatatctt cggcctgcgc ctggtacgtg gggttctgcg gcggcggctg
     5041 gagtttatct ggcgtgtcga tattttgcag gcccgaacat ggttctgctg acgctgataa
     5101 tgcttgccgt tgcgattctg tctacgacag gctgcgtgat gttgggcgat ttcactgaaa
     5161 ggtggtacgg cagaaaggat ccgtcgcatt gtacgctcga cgagtgggcc ggccagtcga
     5221 ttgccctgct gtttctgccg tgcgaatatt cgtggaaaag cacgcttatc ttcgtggcag
     5281 ccgcttttgt cgcgtttcgc gtgttcgaca tcctcaaacc ggcgccagcc agaaatttcg
     5341 agcggtttga caaaggcatt ggcgtggtcg ccgatgacct cgtcgccggc ctctatgcgt
     5401 gcgtgaccgt gcaactgctg ataaaatcgg cagtattcct tccggctgtc aattattgga
     5461 acttttcctg atgagacgcc ttgcggttat atttctgttg ctgtgcgtta ctggcacaac
     5521 attttgtgac caggccgatt ttcagaacac ggcggcggaa gatagaaaaa tatccgtttc
     5581 ggaagccgcc atcctcggta ctattgaagg gctgaccgag tatctgcccg tttcgtccac
     5641 cggccatctg attctggcca atcatgccat gggcaacacg catttttcca gctagatggg
     5701 tcttttcggc cccaaaatcg aatcgacgtc agccgccggc gcatttgaaa tagtgataca
     5761 gttcggtgcg atactggctg ttctgggcct ttatcgcggc cgtgtaa
//
LOCUS       DAONFI010000130         5804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16216_length_5804_cov_4.122630, whole genome shotgun sequence.
ACCESSION   DAONFI010000130 DAONFI010000000
VERSION     DAONFI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16216_length_5804_cov_4.122630"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1159)
                     /locus_tag="PLK08_05395"
     CDS             complement(164..1159)
                     /locus_tag="PLK08_05395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPS52968.1"
                     /translation="MPQAAGWGIIPDMNNNHNHQSGKPQFTVVMPAYNHQNYVEEAVR
                     SVMGQTFTDWELWAVDDGSTDETGKILDRLACEDSRIHVIHKTNGGVSSARNTAIANA
                     RSEWLAYLDSDDVWLPDTLQKYHDFINSNPQADFIYGYRHRMDSAGNKTILDGRFQDA
                     PTSALELFTSQYLSPCRVCHRTELIEKAGNYDENLPACEDYELFLRFSLFTKFYPLGQ
                     PTAWRRRHDTNISRQTGHSRMREAAILRKFADGHPQFITEDVKRRRLGRLYYSAGRQY
                     FKSRCFAQAVQAFRCAEDFSLPLKGKILKTLAFLLTPAGRTDGKKIPTVDNPAGK"
     gene            1236..2198
                     /locus_tag="PLK08_05400"
     CDS             1236..2198
                     /locus_tag="PLK08_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52969.1"
                     /translation="MKRAIFMCILIAGMALPAISQESKPAAKNQDANEKLMNQYQQEI
                     QDLHSIIKQYDAQIRQLKEQIAQLNQQIATLTKSPDDSASAQTDNAVAPAAAAAAQSY
                     WVNLLDMVNLSRDVTAGQWNLNANGLSGNSPQGGMGMIILPVIPTGNYDMTFEFCRTS
                     GSAEIQLDLPVGPRSLRIVLSRPNGWTEDLQYQKYAAGIEYINGMGVLQNQMFSSNNF
                     ILTNGQNHYLHVRIREDGKKAAVDVFYDDTELVDWSGELSSLSMFPDANAQQARKFGL
                     SISAGTMIQIFEARVKMFEKPASPVEPAAPAARVSPVAPAAGGK"
     gene            2333..>5804
                     /locus_tag="PLK08_05405"
     CDS             2333..>5804
                     /locus_tag="PLK08_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52970.1"
                     /translation="MADSNTNRIRPGRRPLQIIVRWVVITLVFLIAAWAFMPLWLPVS
                     GITGSICRDIHERTGLSVTFGSVEASWGKGLILRDFVISSPQGFSQSEPMVKFHRFHC
                     DFSPIRMLLGARLGWCEMEGLEINAEVDSAGNVNLGSLKKLQDDDSGGMNFGRISLTA
                     SKFTLDVAGNPQKLTFGVRNLQFISGRLSNLEQMTMTADVEQAGTAAPVNLQLSAKGS
                     GSPDVARGMLNFVNVDISQIPLPSAESGFPLKSLGGRCDGMLQFHVNKALQVESLTLD
                     LAFNGLAISPAAGEPFEKIPRVTFRTQAALDAIDEGLDISHYEFSMPGLNISGSGDLH
                     SEFFEGNWRGLEFLKLAGVVEPRELRKIFGNHDDIPEHLDGPLAITAFAEIKERTLKF
                     GWNVDAVNARLCRGGDVLKPLGDAMAMSFAGGIGLDDDTNTLAVEKLGVIFGQNVLTG
                     GGRIENLSDLPDLRENYDSVLEILRRLRLHGTMQLAVDGSSKPLNGTWSLDGGERIHI
                     ISTLMADEKTTLKLGDVFVKPAGKASHVDLVATLNALSGEVEKGQLELSCGNSTLQLN
                     DITVRRQAGDGVSGYVIEGQIDGKGLEEFAGFLPQLDGEAELRGEISGTLKGFVDEHV
                     LKPELAVNLNLNRTDIRIGDGFFTKKAGTDFAFDVAAAGIDDHGKVNFRSNVTASDDK
                     DSINLKILASPDERNGIDNATFTLSADNVGMLRRYSPEILGRMNIEDIDGGVTASGVV
                     KKSGVDAGGLEYEIACKPEKISFLLGRNDVTMNGEISLHGKIESRKIQKIFIGELDAG
                     GFDFTFNGDVKKPAGMGVKFSWELAGQDDFINDGGMIHLRSLVGMIGENRFQLAGVIN
                     AGKPFEKSPDLSGTDLHMAINLVDPQTLMTLLPSLASYKPEGDAFLDMQLTSSARDVW
                     LISAVTLKSDGVSGAYNGRRVGLNGSLKLRDVLVKGGRIDAATLNADGLGFSVDGENG
                     YLLADVSMVDSVPSGELSLLMDRFDLKDFADWLAGPNRPQSRPARLTFAEKEELKKQA
                     DAAISQFAKRFAKADLSMKFSFDSFMGYDEAVDKSYLFDKVYASLLIRGGKLAFDYNG
                     GLAGGVSRGSYACDFLADEPRMVWESSVIDVQATPELMPQINKDFPGNTVDGTFTNVV
                     KVYSRLRDFWA"
BASE COUNT         1358 a         1382 c         1637 g         1427 t
ORIGIN      
        1 acgcattaaa attcctgaaa aacactataa aaatttttta aaattcaaga taacaagctg
       61 caagcaaagc tcaatttcca aaattcaagc actctatcta atagcggtta ttcaaatttt
      121 gaaacttgtt ttttgttact tgtagcttat ttttcataag ttactatttc cctgcggggt
      181 tatcaacagt tgggattttt ttgccgtccg ttctgccggc tggcgtcagg aggaaggcca
      241 acgttttgag gattttccct ttcagtggca gtgaaaaatc ttcagcgcac cgaaatgcct
      301 gtacagcctg ggcgaaacag cggcttttga aatattgtct gccggcggaa tagtacaatc
      361 ttccgagccg cctgcgtttt acgtcttccg tgatgaattg cggatggccg tctgcgaatt
      421 ttctcagtat tgccgcttca cgcattcgtg aatggccggt ctggcgggag atgttcgtat
      481 cgtgtcgtcg ccgccatgcg gtaggctggc ccagcggata aaatttcgta aaaaggctga
      541 atcgcaaaaa gagttcgtaa tcttcgcatg ccggcaggtt ttcgtcgtaa tttccggctt
      601 tttcgataag ttccgtgcga tggcatacgc ggcatgggga aagatactga ctggtgaaaa
      661 gttcaagagc ggatgtcgga gcatcctgaa atctgccgtc gagaatcgtc ttgttgccgg
      721 cggagtccat cctgtgtcga tatccgtaaa tgaagtctgc ctgcggattt gaatttatga
      781 aatcgtgata tttctgaagc gtgtccggca gccagacgtc gtcgctgtcc agatatgcca
      841 gccattcgct acgtgcattt gcgatggcgg tgttcctggc ggacgaaacg ccgccgttgg
      901 ttttgtgaat cacgtgtatc cgtgaatctt cgcaggcgag gcggtcgagg attttgcccg
      961 tttcgtcagt tgagccgtcg tcaacagccc atagttccca atctgtgaaa gtctggccca
     1021 tgacggagcg gacagcttct tcgacgtaat tctgatgatt ataggctggc atgacgactg
     1081 tgaactgcgg tttgccggac tgatggttgt gattgttgtt catgtcgggg attatacccc
     1141 agcctgccgc ctggggcaag atgacgataa aatgttgtat gaaccggcaa aaaaattttg
     1201 tatattatgg ccagccttca gacaaggagt cagtaatgaa acgtgcgatt tttatgtgca
     1261 tattaatcgc cggcatggct ttgccggcta tctcccagga aagcaaaccg gcggcaaaaa
     1321 accaagacgc caatgaaaaa ctcatgaatc agtatcagca ggagattcag gacctgcatt
     1381 ccataataaa gcagtacgac gcccagataa ggcagctcaa ggaacagatt gcacaattaa
     1441 atcaacagat agccaccctg accaaatcgc cagatgactc cgcttccgca cagactgata
     1501 atgccgtcgc tccggcggca gccgctgcgg cgcagagcta ttgggtcaat ctgttggaca
     1561 tggtaaatct gtcccgtgac gtaacggctg gccagtggaa tctgaacgcc aacggccttt
     1621 cgggaaattc gccgcagggc ggtatgggca tgataattct gccggtcatt ccgacgggca
     1681 actacgatat gacgttcgaa ttctgccgca caagcggttc tgccgaaatt cagcttgacc
     1741 tgccggtcgg gccgaggtcc ctcaggatag ttctctccag gcctaacggc tggacggaag
     1801 acctgcaata ccagaaatat gcggcaggca tcgaatatat caacggcatg ggcgttttgc
     1861 agaatcagat gttctcgtcc aacaatttca tattgactaa cggccagaat cattatctgc
     1921 acgtacgaat tcgtgaggat ggcaaaaaag cggctgtcga cgttttttat gacgatacgg
     1981 aacttgtgga ctggtcgggc gagctttcgt cgctgtccat gttccccgac gccaacgcac
     2041 agcaggcacg caaattcggg ctgtccatat ccgccggcac gatgatacag atatttgagg
     2101 ccagggtaaa aatgtttgaa aagccagcct cgcccgtcga gccggctgct cctgccgcgc
     2161 gggtttctcc tgttgctccg gcggctggcg gcaagtgagc atttttgtga ctccttcgca
     2221 ggctgttgtt gccatatttg cggccgactc tttttattga tttattaacg aatccgacga
     2281 cgcggattgt tcattttgga tacattacat tatagagggc cgttgactac cgatggcaga
     2341 cagcaacaca aatcgaatca gacccggccg acggcctttg cagataatag ttcgttgggt
     2401 cgtaatcacc ctggtctttc ttattgcggc atgggcattc atgccgcttt ggctgccggt
     2461 gagcggcatt acagggagta tctgcaggga cattcatgaa cgtaccggct tgagtgtcac
     2521 ttttggttct gtcgaggcga gctggggcaa aggccttatt ctcagggatt ttgtcatttc
     2581 gtcgccgcag ggtttcagcc agtctgaacc gatggtcaaa tttcatcgat tccattgtga
     2641 cttttcacct atccgcatgt tgctgggtgc gagactcggc tggtgtgaga tggaggggct
     2701 ggaaatcaac gccgaagtcg attccgccgg caatgtgaat cttgggtcgc tgaagaaatt
     2761 gcaggatgac gattcgggcg gcatgaattt cggacggata tcgttgacgg cgtcgaaatt
     2821 cactctggac gtagccggca atcctcaaaa gctgaccttc ggcgttcgaa atctgcaatt
     2881 tatcagtggc cgtctgagca atcttgagca gatgaccatg actgccgacg ttgaacaggc
     2941 tggcactgcc gcgccggtga atctgcagct atccgcgaaa ggtagcggtt cgccggacgt
     3001 ggcccgcggg atgctgaact ttgtgaatgt ggatatttcc cagattcctc tgccgtcggc
     3061 ggagagcggt tttccgctaa aaagcctggg cggccgctgt gacggcatgt tgcagtttca
     3121 tgtcaacaag gcgttgcagg ttgaatcgct tacgcttgac ttggcgttca acgggcttgc
     3181 gatttcgccg gcggccggcg agccttttga aaaaataccg cgggtgactt tcagaacgca
     3241 ggctgctctt gacgcgatag acgaagggtt ggacataagt cattacgagt tttcaatgcc
     3301 cggcctgaat atatccggca gcggcgattt gcacagcgaa tttttcgagg gcaactggcg
     3361 cggtctggaa ttcctcaagc tcgccggcgt cgtagagccg cgcgaattgc ggaaaatttt
     3421 cggcaatcac gacgatattc cggaacatct cgacggccct cttgccatca ccgcctttgc
     3481 ggagattaaa gaacgcaccc tcaaattcgg ctggaacgtc gatgccgtca atgcgcggct
     3541 ctgtcgcggc ggcgatgttt taaagccgct tggcgatgcg atggccatgt catttgccgg
     3601 cggcatcggc cttgatgacg acacaaacac gctggctgtt gaaaagctcg gcgttatttt
     3661 tggccaaaac gttcttactg gcggcggcag gatagaaaat ctctccgacc tgccggattt
     3721 gcgggaaaat tatgatagcg tacttgaaat attgcgacgt cttagattgc acggcacgat
     3781 gcaactggcg gttgacggct catcgaagcc gttgaacggc acatggtcct tagacggcgg
     3841 cgaacggata catattataa gtacgctgat ggctgacgag aaaacaacgc tgaaattggg
     3901 agatgttttt gtcaagccgg cgggcaaggc ttcgcacgtt gacctcgtcg ctacgctcaa
     3961 tgccctcagc ggggaagtcg aaaaaggcca gcttgaactt tcttgcggca attcgacgct
     4021 gcaactgaat gacattaccg tacgcaggca ggcaggcgac ggcgtgagcg gttatgttat
     4081 tgaagggcag attgacggaa aagggctgga agagtttgcc ggtttcctgc cgcaattgga
     4141 tggcgaggcg gaactgagag gcgaaatttc cggcacgctg aaaggctttg tcgacgagca
     4201 tgtccttaaa ccggaactgg ccgtaaatct gaacctgaac agaacagaca tccgcatcgg
     4261 cgacggattt tttactaaaa aagcaggtac tgattttgct tttgacgtcg ctgccgccgg
     4321 aattgatgac cacggaaaag tgaattttcg gtcgaacgtg acagccagtg acgataagga
     4381 ctccatcaac ctgaaaattc ttgcctcgcc ggacgagcga aacggaatcg acaacgcgac
     4441 gtttacgctg tcggctgata acgtcggcat gttacggaga tattcgcctg aaattctggg
     4501 cagaatgaat atcgaggata tcgacggcgg tgtgacggcg agcggcgttg tgaaaaaatc
     4561 cggagttgac gcgggtggcc ttgaatacga aattgcgtgc aagccggaaa aaatttcatt
     4621 tcttctcggc agaaatgacg tgacgatgaa tggcgagata tcgcttcacg gcaaaataga
     4681 aagccgtaag attcagaaaa tttttatcgg cgaacttgat gccggcggat tcgattttac
     4741 ctttaacggc gatgtcaaaa agccggccgg catgggcgtg aaattttcat gggaactcgc
     4801 cggccaggat gattttataa atgacggcgg catgattcat cttcgcagcc ttgtgggcat
     4861 gataggggag aatcgttttc agctcgccgg cgtaataaat gcaggcaaac cgttcgaaaa
     4921 atcccccgac ctttccggca ccgatttgca tatggcgata aatctcgtcg acccgcagac
     4981 tttgatgacg ctgttgccga gcctggcgtc ttacaagccc gaaggcgatg cgtttctgga
     5041 tatgcagttg acgtcctccg cacgcgatgt gtggttaatc agtgccgtga cgctgaagag
     5101 tgacggcgta agcggtgcgt ataatggccg gcgagtcggg ctcaacgggt cgcttaaatt
     5161 gcgagacgtg ctcgtgaagg gcggcaggat tgacgctgcg acgctgaatg ccgacggatt
     5221 gggtttttct gtggatggcg aaaacggtta tctgctggct gacgtttcca tggtcgattc
     5281 cgtgccgtcg ggagagcttt cgctgttgat ggacaggttt gacctgaagg attttgccga
     5341 ctggctggct ggcccgaatc ggccgcaatc acggccggca aggctgacct tcgcggaaaa
     5401 ggaagaactg aaaaaacagg ctgacgcagc catatcgcag tttgcaaaac gcttcgccaa
     5461 ggctgacctg tcaatgaaat tttcattcga cagttttatg ggttacgacg aggcggtcga
     5521 caaaagttat ctgttcgata aggtttacgc gtcgctgctg attcgcggcg gcaagctggc
     5581 gttcgattac aacggcggat tagccggcgg cgtctcccgc ggttcttacg catgcgattt
     5641 ccttgcggat gagccccgca tggtctggga gagcagcgtt atcgacgtgc aggctacgcc
     5701 ggaacttatg ccgcagatta acaaggattt tccaggcaat acggttgatg gcacatttac
     5761 gaacgtcgtg aaagtttaca gccgtctgcg ggatttttgg gcga
//
LOCUS       DAONFI010000131         5802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16225_length_5802_cov_4.179050, whole genome shotgun sequence.
ACCESSION   DAONFI010000131 DAONFI010000000
VERSION     DAONFI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16225_length_5802_cov_4.179050"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLK08_05410"
     CDS             complement(<1..897)
                     /locus_tag="PLK08_05410"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPS52971.1"
                     /translation="MTQKSSKYSAPKKFEMAQRDWPGKEITTAPQWCSVDLRDGNQSL
                     PNPLSPSAKLEYFQLLCEIGFKHIEIAFPSASHDDFTFTRKLIEEDFIPADVFIMGLV
                     QCRPHLIERTFESLKDVRRAIVHAYTACSPLHREKVLHMSRQQTLEMAVNAITQIRQL
                     ADAMPQSDIRAEFSPEEFTDTEMDFSLEICTAVYEAWGKASPTKPLILNLPATVERRP
                     PNHYADMIEIFCKQFKYRQNTIISVHAHNDQGMAVAATELALLAGADRVEGTLFGHGE
                     RTGNVDIVTLANNLASRGIETGL"
     gene            1294..1791
                     /locus_tag="PLK08_05415"
     CDS             1294..1791
                     /locus_tag="PLK08_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPS52972.1"
                     /translation="MSKRCVECKAKCCTYFCFQIDTPETFEAFEDIRWYLCHDGVSVH
                     IDEDDDWYIQITNRCNMLSDDCRCRIYDDRPLICRQYSADNCEGNPEIGEDYGYKQEF
                     KTPGQLDRYAFRTLGRAEYENEMISHRAGHAGVAPKVMKAELLKAGMLPRKEFFDKNQ
                     ARVKK"
     gene            1788..3089
                     /gene="hisS"
                     /locus_tag="PLK08_05420"
     CDS             1788..3089
                     /gene="hisS"
                     /locus_tag="PLK08_05420"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPS52973.1"
                     /translation="MKFQAVKGTRDFYPEEMRVRNWIIDAWRKVSVRNGFVEYDAPIF
                     EYLELFTVKSGEEIAEQLFNFTDRGGRNIAIRPEITPSLARMIAAKINSLPRPIKWFS
                     VPRVCRAERPQRGRLREFFQWNVDIVDENSVQADAECIYVCIDFLREVGLTPADVKIH
                     IGHRPLIMAMLRGRGVSDENMDKALLALDKRPKISHEAFVEMLENANLAPAGIQGVCE
                     FMDTSSADALSVLDTIDGMEEHKRNLRELGAALENMGVGEYVTFDWHIVRGLAYYTGA
                     VYEALAAGERAVAGGGRYDKLLEIVGGPAASATGFGMGDVVLGILLQEKGLIPAKILC
                     ESLDYYIIDGGEGTFPAVLKLAGELRLRGATADYSFRRQNVGKQLKEANRRGAKAAVI
                     VENPDITAEGSITIKNLATGEQASATSAEILGRHDGTSNTL"
     gene            3064..3594
                     /locus_tag="PLK08_05425"
     CDS             3064..3594
                     /locus_tag="PLK08_05425"
                     /inference="COORDINATES: protein motif:HMM:NF038001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HPS52974.1"
                     /translation="MTGRRIHYEDAFEDFLRTQGIPYVAVDDDKQTIFGKASIKSFDF
                     VVYSHEGPNLIVDIKGRKFPDVATAGAKRSTRAWENWITRQDLDGLTRWQDIFGSDFQ
                     VVLVFAYWLQGKVNTSPFDDVYFYNNSYYAFMAMPLSAYVSFAQPRSEKWQTIAVPSR
                     IFADNAVDIRRMLNVL"
     gene            3654..5327
                     /gene="ettA"
                     /locus_tag="PLK08_05430"
     CDS             3654..5327
                     /gene="ettA"
                     /locus_tag="PLK08_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPS52975.1"
                     /translation="MPPQFVFEMHDVCKSYGERQVLRDISLSFFYGAKIGVVGENGAG
                     KSTLLKIMAGIDKDFQGETRLAPKMRLRYVAQEPQLELDRTVRENLLTAVKPTLDMID
                     RFNEISERMGDLGPDDDMDKLLDEMGTLQDKIDACDGWETDRLIDIASDALVLPPDDT
                     PVRQLSGGERRRVALCMALLEKPDLLLLDEPTNHLDAETVQWLEAALREYHGTVIIVT
                     HDRYFLDNITRWILELENGLGLPFEGNYSSWLKQKAELLRLIEKKETERQKTLARELE
                     WINTSARARNQKNQARINAYEKLSGQNSSDARSEVVIQIPGGQRLGDKVLTFHDVTKG
                     YDGSTLIEKCSFDLPRNAIVGVIGPNGIGKTTLFRMIVGQERPDSGRVEVGPTVSIGY
                     VDQHRDALDDERTIFEEITDGKDNIDVGGRAVPSRAYVARFNFRGSQQQKKVSECSGG
                     ERNRIHLAKMLRRGGNLLLLDEPTNDLDVDTMRVLEQAIIDFEGCAMVISHDRFFLDR
                     ICTHLLIFEGDGKTQWFQGNFSAYEEKVQAENPDRVAHRRGKYKRLRLR"
     gene            complement(5367..>5802)
                     /locus_tag="PLK08_05435"
     CDS             complement(5367..>5802)
                     /locus_tag="PLK08_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPS52976.1"
                     /translation="DVRIYSVPTPHDAADGCCFVVEAEGRRLGILTDLGHVFSGLADV
                     METLDALLLESNYDPVMLRSGFYPAFLKARIAGPHGHLSNDEAATLVKNHGRRLNWLC
                     IGHLSDENNTPELALTTNRKIIGSSLPIFLAGRHGISETMEV"
BASE COUNT         1408 a         1371 c         1639 g         1384 t
ORIGIN      
        1 caggccggtc tctatgccgc gcgacgccag attgttagcc agcgtcacta tatccacatt
       61 gccggttcgt tcgccgtggc cgaatagcgt gccttccacg cgatccgcgc cagccagcaa
      121 tgccagctcc gtcgcagcca ccgccatgcc ctggtcgtta tgggcgtgaa cggatattat
      181 ggtattttga cgatatttga actgtttgca gaatatttct atcatgtcgg cgtagtgatt
      241 cggcggccga cgttcaacgg tcgctggcag gttcagaatt aaaggctttg ttggtgaggc
      301 tttgccccac gcttcataga ccgcagtgca gatttccagc gaaaaatcca tttccgtgtc
      361 ggtgaattct tcgggactga attctgcacg gatgtcgctt tgcggcatgg cgtcggctaa
      421 ctgtcgaatt tgagttatcg cattgacggc catttccagc gtctgctgac gggacatgtg
      481 caaaaccttc tcgcgatgca gcgggctaca cgcggtatag gcgtgcacga tagcccgtct
      541 gacatccttg agcgactcga aagtacgttc tatcaggtgc ggccgacatt gaaccaggcc
      601 catgatgaaa acgtcggctg gtatgaaatc ttcctctatc agcttgcggg tgaaggtgaa
      661 gtcatcatgg ctggctgacg ggaaggcgat ttctatatgt ttgaagccta tctcgcaaag
      721 caactggaag tattcaagtt tggctgacgg cgaaagcggg ttgggcagtg actgattgcc
      781 gtcgcgcagg tcaacagaac accactgggg ggctgtggta atttctttac caggccaatc
      841 tctttgggcc atctcgaatt tcttcggagc tgaatacttc gaactctttt gcgtcatctc
      901 tgactcctta cattatttga aaaacaaaaa aagctttgca atccggtcgg actgcaaagc
      961 ctgtcaaaat tttttcaggt tttttagtcc gtcagcgcag cataagtcca agaaggaggg
     1021 ctagggacaa attgttgctc tgacgaatac tcataactga agtataatct accagccggc
     1081 cggattacaa gaatttttta atttttttaa atctgtctac taccgtataa atgtgcaaat
     1141 cagtttgagt ttgaaaagtg agtgaaaaac gctatattaa agcgattctc aaaagaagat
     1201 tcttttgaaa accactctga acgcttttgc gttttttagt gaaaagatgc aaacgcagtc
     1261 ctgctttaat taaataagga tgaggtttta gaaatgtcga aacgctgtgt ggaatgcaag
     1321 gcaaaatgct gtacctattt ctgtttccag atagatacgc ctgaaacgtt cgaagccttt
     1381 gaggatatcc gctggtatct gtgccacgac ggcgtatccg tacatatcga cgaggatgac
     1441 gactggtaca ttcagataac caaccgctgc aacatgctca gcgatgactg tcgatgcagg
     1501 atttacgatg ataggccgct gatttgccgc cagtattccg ccgataattg cgagggcaat
     1561 cctgaaatcg gcgaagatta cggctataag caggagttca aaactcccgg ccagctggat
     1621 cgttatgcgt tcaggacgct tggcagggct gaatatgaaa atgaaatgat atcgcatcgt
     1681 gccgggcatg ccggcgttgc gccgaaggtc atgaaggctg aattgctgaa agccggcatg
     1741 ctgccgcgaa aggaattttt tgacaaaaat caagccaggg tgaaaaaatg aaatttcagg
     1801 ccgtcaaggg aacgcgggat ttttatcccg aagaaatgcg tgtgcgaaac tggataatcg
     1861 acgcatggcg aaaagtgtcc gttcgtaacg gctttgtgga gtacgacgcg ccgatattcg
     1921 aatatcttga gcttttcacc gtcaagagcg gcgaggagat agccgagcag cttttcaatt
     1981 ttaccgaccg cggcggccgc aatattgcca tccggcctga aataaccccc tctctagccc
     2041 gcatgatagc ggcgaagata aattcgctgc cgcggcctat aaaatggttt tccgtgccgc
     2101 gtgtctgccg cgctgaacgg ccgcagcggg gccgtctgcg tgaatttttc cagtggaatg
     2161 ttgatatcgt cgatgaaaat tccgtgcagg ctgacgcgga gtgcatttac gtctgtatcg
     2221 attttctgcg tgaagtcggc cttacgccgg ctgacgttaa aattcacatc ggccatcggc
     2281 cgcttatcat ggcaatgctt cgcggcagag gcgtaagcga tgaaaatatg gacaaggcgc
     2341 tgctggcgct ggataaacgg ccgaaaattt cacatgaggc gtttgtggaa atgctggaaa
     2401 acgccaacct tgccccagcc ggtattcagg gcgtctgtga atttatggat acgtcgtcag
     2461 ccgacgcgct gtcggtactt gacactatag acggcatgga agagcacaag cgtaatctgc
     2521 gtgagctcgg tgcggcgctt gaaaatatgg gcgtcggcga atatgtgact ttcgactggc
     2581 acatcgtgcg agggttggcg tattacaccg gcgcagttta tgaagcactt gccgccggcg
     2641 agcgtgcggt ggctggcggc ggccgttatg ataaacttct ggaaatcgtc ggcgggccgg
     2701 ctgccagcgc tacgggcttt ggcatgggag atgttgtgct gggaattctg cttcaggaaa
     2761 aggggctgat tccggcgaaa atcctctgcg aatcgctcga ttattacata attgacggcg
     2821 gcgagggtac atttccggca gtgttgaaac ttgcaggcga attgcgattg cgaggagcga
     2881 cggcggatta ttctttccgc aggcagaatg tcggcaagca gctcaaggag gctaaccggc
     2941 gcggtgcgaa agcggcagtt attgtggaaa atcctgatat taccgctgag ggcagtatta
     3001 cgataaaaaa tcttgcaacg ggagagcagg catccgccac gtcggcggag attctgggca
     3061 ggcatgacgg gacgtcgaat acattatgaa gacgcattcg aggattttct gcggacgcag
     3121 ggaattccgt atgtagccgt cgatgacgat aaacagacta tcttcggcaa ggcatccatc
     3181 aaaagttttg acttcgtcgt atattctcac gaagggccga atctgattgt ggatatcaag
     3241 ggccgaaaat ttcctgatgt cgccacggcg ggggcgaaac gttcgacgag ggcgtgggaa
     3301 aactggataa cgcgacagga tctcgatgga ttgacgcggt ggcaggatat tttcggaagc
     3361 gattttcagg ttgttctggt ttttgcatat tggttgcagg gcaaggttaa tactagcccg
     3421 tttgacgatg tgtattttta taataacagc tattatgcgt tcatggccat gccgctgtcg
     3481 gcttacgttt cctttgccca gccgaggagt gaaaagtggc agaccatagc cgtgccgtcg
     3541 cgtattttcg ccgataatgc cgtcgatata cggcggatgt tgaatgtcct gtgatggccg
     3601 taaacgttgg ctgtcttctc aattctttta tttttgcctg atgaggaaca gatatgcccc
     3661 cgcaattcgt ttttgaaatg catgacgtct gcaagagcta tggcgagagg caggtgctgc
     3721 gcgatatttc actatcgttt ttctacggcg ccaaaatcgg cgtagtcggt gaaaacggag
     3781 ccggcaaaag cacgctgctt aagataatgg ccgggattga taaggatttt cagggtgaaa
     3841 cccgacttgc cccgaaaatg cggctgcgat acgtcgcgca ggaaccacaa cttgagctgg
     3901 acagaactgt ccgcgaaaat ctacttaccg ccgtcaagcc cacgctggac atgattgacc
     3961 gttttaacga gatatccgaa cgcatgggcg accttgggcc tgatgacgac atggacaagc
     4021 tgctcgatga gatgggtacg ctgcaggata aaatcgacgc gtgcgacggt tgggagactg
     4081 accggctgat tgatattgcg tccgatgcac ttgtacttcc gccggacgat acgccggttc
     4141 gccagctttc cggcggcgag cgaagacgcg tcgcgctttg catggccctg ctggaaaagc
     4201 ccgacctgct ccttctggac gaacctacca accatcttga cgcggaaacg gtgcaatggc
     4261 tggaggctgc actgcgggag tatcatggca cggttatcat cgtcacgcac gacagatatt
     4321 ttctggacaa tatcaccagg tggatacttg agcttgagaa cggcctgggt ctgccgttcg
     4381 aagggaatta ttcatcgtgg ctgaaacaga aggctgaact gctgcggctt atcgagaaga
     4441 aagagaccga gcggcagaaa actctcgctc gcgaacttga gtggataaac acatccgcca
     4501 gggctcgtaa tcagaaaaac caggctcgca tcaatgccta cgaaaaactc tccggccaga
     4561 attcgtccga cgcgcgcagt gaggtcgtca tacagattcc cggaggtcag aggctgggcg
     4621 ataaggttct gacgtttcac gacgttacca aaggttacga cggcagcacg cttattgaaa
     4681 aatgttcgtt tgatttgccc cgcaatgcca ttgtcggcgt aattgggccg aatggtatcg
     4741 gcaagacaac gctgttcagg atgattgtcg gtcaggaacg gcccgattcc ggcagggtgg
     4801 aagtcgggcc gacagtgtcg ataggttacg tcgaccagca ccgcgacgcg ctcgacgacg
     4861 aacggacgat ttttgaggaa atcaccgacg gcaaggacaa tatagacgtc ggcggcaggg
     4921 cagtaccgtc ccgcgcttat gttgcgcgat tcaacttccg cgggtcgcag cagcagaaaa
     4981 aagtatcgga atgttcagga ggcgagcgaa acaggattca cctcgccaaa atgcttcgtc
     5041 gcggcggcaa cctgttgctg ctagacgaac cgacgaatga tttggacgtg gatacgatgc
     5101 gagtgttgga gcaggcgata atagattttg aaggctgtgc catggtcatc agccacgacc
     5161 gttttttcct tgaccgcatc tgcacgcatc tgctgatatt cgagggcgac ggcaaaacgc
     5221 aatggtttca gggtaacttt tctgcctatg aagaaaaagt gcaggcggaa aatccagacc
     5281 gtgtcgccca tcgacgtggg aaatataaac gcctgcggtt acgatagttt tttaagttaa
     5341 caaagaattt atgaaagcga taaaaatcag acttccatag tttcgcttat gccgtggcgg
     5401 ccggcgagga aaatcggcag ggagctacct atgatttttc gattagtcgt aagggcaagt
     5461 tccggcgtgt tgttttcgtc ggaaaggtgg ccgatgcaca gccagttcag gcggcggcca
     5521 tgatttttga cgagcgtggc agcttcatcg tttgacaggt ggccgtgcgg gcctgctatg
     5581 cgagccttca gaaatgccgg ataaaatccg cttcgcagca tgacagggtc gtagttgctc
     5641 tccaatagca gggcgtcgag agtttccata acatctgcca gccctgagaa aacatggccc
     5701 aggtcagtca gaatccccag ccgcctgccc tctgcctcca ccacgaaaca gcagccgtcg
     5761 gccgcatcgt gcggcgtggg tactgaatat atccgtacgt cg
//
LOCUS       DAONFI010000132         5747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16405_length_5747_cov_4.197822, whole genome shotgun sequence.
ACCESSION   DAONFI010000132 DAONFI010000000
VERSION     DAONFI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16405_length_5747_cov_4.197822"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLK08_05440"
     CDS             <1..199
                     /locus_tag="PLK08_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HPS52977.1"
                     /translation="VLCGPACERLKKAPVDEVVVTDTIPVGQEKIDAIGKLRVLSVAP
                     LLGEAIHRIHNNESVSSLFVH"
     gene            285..950
                     /locus_tag="PLK08_05445"
     CDS             285..950
                     /locus_tag="PLK08_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPS52978.1"
                     /translation="MAKITATTRKENGSNAVKRLRAAGKVPGNVYGHGKAPQSLTLDK
                     HEIALAIQHHERVLDMVVDGKDENVMFKEVQYDSLGDNILHVDFSRVNMDEIVRVTVP
                     VNFIGTAQGMKDGGVLQIACSDVTIECTVRNMPEEIRHNIEAVPLNGHVYAHDLTLPE
                     GARLVDDENEVLCSVAIIAEEAVAEPAAEGETPAEPEVIGEKKEEEGEEAKADTASKP
                     KKE"
     gene            957..1568
                     /gene="pth"
                     /locus_tag="PLK08_05450"
     CDS             957..1568
                     /gene="pth"
                     /locus_tag="PLK08_05450"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPS52979.1"
                     /translation="MASDPQCPARRFVVGLGNPGRKYVETRHNVGFRVLEVLRQRWGL
                     ETAAEKFQSMFWQTRRERFDEESRNVVLLAPMTYMNESGRAVRAMMDFYKAQPDEILV
                     VLDDFALPLAQLRLRAKGSAGGHNGLADIIHKCGSDEFPRLRIGIGTPPGVMDVSDYV
                     LGKFASNELEDIGSAINIAADAVEDWVFGDMLNVMQKYNRKSD"
     gene            1619..2056
                     /gene="rpsF"
                     /locus_tag="PLK08_05455"
     CDS             1619..2056
                     /gene="rpsF"
                     /locus_tag="PLK08_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPS52980.1"
                     /translation="MEQRINTYEAMFLLDPGQMNFEAACQPINRVFERNGAEVLQLKL
                     WDERKLAYEIKNRKRGLYVISYFKMDTANVQALERDCMLSDEIIRVLVIRKDRLTEEE
                     MNAETPAETAANAPVEFSGSDYANEQSDDQEDDTVSVDDAVEE"
     gene            2109..2621
                     /gene="ssb"
                     /locus_tag="PLK08_05460"
     CDS             2109..2621
                     /gene="ssb"
                     /locus_tag="PLK08_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPS52981.1"
                     /translation="MASFNKVILMGNLTRDPQLSYLPSQTPVVEVGLAVNRRFRTNDG
                     QQREDTCFIDCRAMGKQAETINQYLRKGSPILIEGRLEFDQWEAQDGTKRSKHRVFIE
                     RFTFVGGPAGGAPMGGGYQQSQSGYNQPAPAPAPQAQTPAPQQQNRAPMPQQPPYYEE
                     DSPGGEDIPF"
     gene            2694..2942
                     /gene="rpsR"
                     /locus_tag="PLK08_05465"
     CDS             2694..2942
                     /gene="rpsR"
                     /locus_tag="PLK08_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPS52982.1"
                     /translation="MTKGKMVRRRKKARVVEQAKCRFCRDKVQEVDYKDINTLIKLTT
                     QQGKLFSRKRSGNCARHQRSCMAAIKRARFLGLMPYVG"
     gene            2984..3454
                     /gene="rplI"
                     /locus_tag="PLK08_05470"
     CDS             2984..3454
                     /gene="rplI"
                     /locus_tag="PLK08_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPS52983.1"
                     /translation="MKVLLRKNVYKLGQIGDVVEVKNGYGRNFLIPQGMAYIPSEANI
                     RAVENEKARYLEELARQRTELETRAKAVNGKEVTITARANSEGVLYGSVGPAQIAAAF
                     SEIDVHINPDEIVMHEPIRKLDRHEVNVRLGQEVKAVVYVWITPLATAGEESAE"
     gene            3623..5020
                     /gene="dnaB"
                     /locus_tag="PLK08_05475"
     CDS             3623..5020
                     /gene="dnaB"
                     /locus_tag="PLK08_05475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPS52984.1"
                     /translation="MEETKTMNDEPKNASSADTDRLGREVPADLESEACVLGSMIIDS
                     EVIDIVVQILKSEYFHRPAHAIIYDVLLDMQDKRQPIDLVTIKEELTRRKLLEKIGGI
                     DYIVALAEGVPGVSNVEYYANIVRDKALLRRLIRVSQEIITDIYDSTDEAQIVIDQAE
                     KKIFEIAEQKIGNDVVGLKEMLDKTFKDLEDNEGKTITGLASGYLQLDEMLAGFQNSE
                     LIIVAARPSMGKTALLLNFAEHVSITDKKPIAFFSLEMARLQITQRLLSSYSQFNLRN
                     LRRTAISPEDWTVLQSAAGQLYTAPFYIDDTSVLTPLQLRAKARRLKARFDIKAIFID
                     YMQLMSPSGRGSDKRYEQIGEISRSLKALARELDIPVIVAAQLNRGPTDRTEHTPRMS
                     DLRESGSIEQDADVVMLLHNEDYYHRGDPDYVPENITRLIVEKQRNGPTGVVKLIFRP
                     ECTRFESAAPSFMDN"
     gene            complement(5143..>5747)
                     /locus_tag="PLK08_05480"
     CDS             complement(5143..>5747)
                     /locus_tag="PLK08_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPS52985.1"
                     /translation="LQSAAEVADEFLSEGKIVYTRGLLVPRTDIDAYKPGLFENGNLV
                     VIVDQGSASASEILSGALKDHARAKIIGQRSYGKGSVQNIITVYPNTAFLKLTTAYYY
                     LPSGRLLHRTPESKDWGVNPDVSVFMTPRQTLDWMKLRYRTDLLQDVLPQIMAEDLKM
                     QYNADIQLNTAVLMLKLMQLKDKAANDYTRLAGKVKAGES"
BASE COUNT         1546 a         1505 c         1521 g         1175 t
ORIGIN      
        1 cgtcctttgc ggccctgcat gtgaacggct gaaaaaagcc cccgtggatg aagtggtggt
       61 gaccgacacg ataccagtcg ggcaggaaaa aatcgacgcc atcggcaaac tgcgcgtcct
      121 gagcgtggcg ccgctgctcg gcgaagccat ccatagaata cacaataacg aatcggtgag
      181 cagcctgttc gttcactgaa aagtttttat ttgtcaattt attgaaaaat caacagttag
      241 gttgccctgg cgcggtgccg gggtacgaca aggagtaatt cgcaatggcg aagataacag
      301 ctacgacacg taaggaaaac ggctcgaatg cggtaaagcg tttgcgtgca gcgggcaaag
      361 tgcctggcaa tgtttacggc cacggcaaag ccccgcagtc tctgacgctc gacaaacacg
      421 aaatcgcact ggccattcag catcacgaac gcgtgctgga catggttgtc gacggcaaag
      481 acgaaaacgt catgttcaag gaagtgcagt acgacagcct cggcgacaat attctgcacg
      541 ttgacttcag ccgcgtgaac atggacgaaa tcgtccgcgt aaccgtgccg gtcaatttca
      601 tcggcactgc acagggcatg aaagacggcg gcgtcctgca gatagcctgc tccgacgtta
      661 ccatcgaatg taccgttcgc aatatgcccg aagaaatccg tcacaatatc gaggcggtgc
      721 cgctcaacgg ccacgtttac gcccacgacc tgaccctgcc cgaaggcgcc agactcgttg
      781 acgacgaaaa tgaagttctc tgctctgtgg ccataatagc agaagaagca gtcgccgaac
      841 cagccgccga aggcgaaacg ccagccgagc cggaagtcat cggcgaaaag aaggaagaag
      901 aaggcgaaga ggccaaagcc gataccgcct caaagcccaa aaaggagtaa cgccgtatgg
      961 ccagcgaccc tcaatgccct gcacgcagat ttgtcgtcgg gctgggcaac ccgggacgga
     1021 agtatgtcga aacgcggcat aacgtcgggt tcagggtgct ggaggttctt cggcagcgtt
     1081 gggggctgga aacggcggct gaaaaatttc agtcgatgtt ctggcagacc cgtcgcgagc
     1141 ggttcgatga ggaatcgcga aacgtggtgc ttttggcgcc catgacatat atgaacgagt
     1201 caggccgcgc ggttcgggca atgatggatt tttacaaagc ccagccggat gagatactcg
     1261 tcgtcctgga cgatttcgct ctgccgctgg cacagttgcg gctcagggcg aaaggttcag
     1321 ccggcggcca taacggactt gccgatatta ttcacaaatg cgggagtgat gaattcccgc
     1381 ggctgagaat aggaataggc acaccgccag gcgtaatgga cgtcagcgat tacgtgctgg
     1441 gcaagttcgc atcaaatgaa cttgaagaca taggcagtgc gataaacatc gccgccgatg
     1501 ccgtcgaaga ttgggtcttt ggcgacatgt tgaatgtaat gcagaagtac aatcgcaaaa
     1561 gcgattgacg taatgttaac gttaggctta tatcagccca aaaaacggaa aagctatcat
     1621 ggaacagaga atcaacactt atgaagctat gtttctgctg gaccccggcc agatgaactt
     1681 cgaggcggcc tgccaaccga taaatcgcgt tttcgaacgc aacggcgccg aagttctgca
     1741 actgaagctc tgggacgagc gtaaactcgc atatgaaatt aaaaatcgca aacgcggctt
     1801 gtacgtaatc tcctacttca agatggatac cgccaacgta caggcgctgg agcgcgactg
     1861 catgctgagc gatgaaatca tccgcgtgct ggtgattcgc aaggatcgcc tcactgaaga
     1921 agagatgaac gccgagaccc ccgcggaaac ggccgccaat gctccggtgg aattctccgg
     1981 ctcggattac gccaatgaac aatccgacga ccaggaagat gacaccgtca gcgtcgacga
     2041 tgccgtcgaa gagtaaccag gtggtacgtt ttacccttaa aaatcatcga actaacgaga
     2101 ggtgacgtat ggccagcttt aataaagtta tattgatggg aaatcttacg cgtgacccgc
     2161 aactgtcgta tctgcccagc cagacgccgg tcgttgaggt aggtttggcc gtaaaccgtc
     2221 ggttcagaac taacgacggc caacagcgtg aagacacctg ctttatagat tgcagggcaa
     2281 tgggcaagca ggctgagacc atcaaccagt atttgcgtaa aggctcgccg atactgatag
     2341 aaggccggct tgagttcgat cagtgggaag cgcaggacgg cacaaagcgc agcaagcatc
     2401 gcgtttttat cgaaagattc acattcgtag gcgggccagc cggcggcgca ccgatgggcg
     2461 gcggatatca gcagtcgcaa agcggttaca atcagccagc cccagctcca gcaccgcaag
     2521 cacagacgcc ggctccgcag cagcaaaacc gtgctccaat gccgcaacag ccgccatact
     2581 atgaagaaga ttcacccggc ggagaggaca ttcctttctg accgctgaga tagaagtaaa
     2641 aattttttaa aaaaattatc aggtaaatca agaggataaa tatcatggct aatatgacca
     2701 aaggcaaaat ggttcgtcgc cgcaagaaag cccgcgttgt cgagcaggca aagtgcagat
     2761 tctgccgcga caaggttcag gaagtcgatt acaaggacat aaacacactt atcaagctca
     2821 ccacgcagca gggcaagctc ttcagccgca aacgcagcgg caactgcgcc cgccatcaac
     2881 gctcgtgcat ggccgccata aagcgtgcca gatttctcgg cctcatgccg tacgtcggct
     2941 gagctggtaa gttcgctatt tatttatcag gaaggataac gccatgaagg tgctactcag
     3001 aaaaaacgta tataaactcg gtcagatagg tgacgtggtt gaagtaaaga acggctatgg
     3061 ccgtaatttt ctcataccgc agggaatggc ttatattccc agcgaggcga acattcgtgc
     3121 agttgaaaac gagaaagccc gctacctgga agaacttgcc cggcagcgta ccgagctgga
     3181 aacgcgcgcg aaggctgtca acggcaagga agtcaccatt accgcccgcg ccaattccga
     3241 aggcgtactt tacggttctg tcggaccggc gcagattgcc gccgcattca gcgaaatcga
     3301 cgttcacatc aaccccgatg aaatcgtcat gcacgagccg atacgcaaac tcgacagaca
     3361 tgaagtcaac gtacgtctcg gccaggaagt caaagccgtc gtttacgtat ggattacgcc
     3421 gctggctacc gccggcgaag agtctgccga ataatacaac acatatcagc catcaaaacc
     3481 atctgtgccg gaaaaatatc cggcgccgat ggttttttta tgctgcggtg cataacctgt
     3541 tgattgctgt tggaatctgt cggcaactcg tttattttgc ctagcctatt gaattaaatc
     3601 tgccgaaagg tagattgtcc gtatggaaga aaccaagaca atgaacgatg agccgaagaa
     3661 cgcttcgtcg gccgataccg acaggctggg ccgcgaagtt ccggcagacc tcgaatcgga
     3721 agcctgcgtg ctcggctcga tgattatcga cagcgaagtc atagatatcg tcgtacagat
     3781 tctcaaaagc gaatatttcc accgtcccgc ccacgccata atatacgacg tcctgctcga
     3841 catgcaggac aagcgtcagc caatcgacct ggtaaccatc aaggaagagc tcacgcggcg
     3901 caagctgctg gagaaaatcg gcggcatcga ttatatagtg gctctcgcgg aaggcgtgcc
     3961 cggcgtcagc aacgtggagt attatgccaa catcgtcagg gataaagccc tgctgcgacg
     4021 gctaatccgc gtcagccagg aaatcatcac cgatatttac gattcaaccg acgaagcaca
     4081 gattgtaatc gaccaggctg aaaagaaaat ttttgaaatc gccgagcaga agataggtaa
     4141 cgacgtcgtc ggccttaaag agatgctgga taagacattc aaagatctcg aagataatga
     4201 gggcaagacg attaccggcc ttgccagcgg atatttacaa ctcgacgaga tgctcgccgg
     4261 tttccagaat agcgaactga ttatcgtggc ggctcggccg tcaatgggca agacggctct
     4321 gctgctgaat tttgccgaac atgtttcaat cacggacaaa aaacccatcg cctttttttc
     4381 actcgaaatg gcccggctgc agataactca gcgtctgctg agcagttatt cgcagttcaa
     4441 tctgcgaaat ctgcgtcgca cggcaatttc gccggaggac tggacggtac tccagtccgc
     4501 cgccggccag ctctacacag ctccgtttta cattgacgac acatccgttc ttacgccgct
     4561 gcagcttcgt gccaaagctc gccggctaaa ggccagattt gatataaagg cgatatttat
     4621 cgactatatg cagcttatga gtccatccgg acgcggctcg gacaagcgat atgaacagat
     4681 aggtgaaata tcccgcagcc tcaaggcatt agcccgcgaa ctggatatac cggttatcgt
     4741 cgccgcacag ctcaaccgcg gcccgaccga ccgtacggaa catacgccgc gaatgagcga
     4801 cctgcgcgaa tccggctcta tcgaacagga cgccgatgta gtcatgctgc tgcacaacga
     4861 ggattactat catcgcggcg atccagatta cgtgccggaa aatatcactc gcctgattgt
     4921 tgaaaaacag cgtaacggcc cgactggcgt ggtcaaactc attttcaggc cggaatgcac
     4981 gcggttcgaa tcggcggctc caagtttcat ggataactga tttttcgagc ggtttacgtc
     5041 aggatatgca agacgacaaa aacctgaatc gccggcatca tctttacgga cgatgccgga
     5101 gtattaaaag gtcacatttt ctggaaccga tataaaaaaa tatcaggatt cgccggcttt
     5161 gactttaccg gcaagacggg tgtaatcgtt agccgcctta tccttgagct gcatgagttt
     5221 cagcatcagc acggctgtat ttaactggat atccgcatta tattgcatct tgagatcttc
     5281 agccatgatt tgcggcagaa catcctgaag caaatcagtc ctgtatctga gtttcatcca
     5341 gtccaatgtc tgccgcggcg tcataaagac gctgacatcc ggattgacgc cccaatcctt
     5401 cgattccggc gttctgtgca acagacggcc agacggcaga taataatacg ccgtcgtcaa
     5461 tttaagaaac gccgtattcg gatagacggt tatgatattc tgcacgctac ctttgccgta
     5521 cgaccgctgg ccgataatct tggcacgagc atggtctttc aacgctccgc tgagaatttc
     5581 agacgcactc gcagaaccct ggtcaactat gacaacaaga ttcccgttct cgaaaaggcc
     5641 gggcttgtag gcgtctatat ccgtccgcgg aacaagaagg ccgcgagtat aaactatttt
     5701 gccttcgctg aggaattcgt cagccacttc agccgcactc tggagca
//
LOCUS       DAONFI010000133         5742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16432_length_5742_cov_3.895903, whole genome shotgun sequence.
ACCESSION   DAONFI010000133 DAONFI010000000
VERSION     DAONFI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16432_length_5742_cov_3.895903"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLK08_05485"
     CDS             <1..407
                     /locus_tag="PLK08_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52986.1"
                     /translation="GQEIKADEGINPAVFSQIKKTLGNDLLLDKSRMSGKLLQGEDNA
                     KEGLAIFKDFDPQAGSFDIFIGGLCSNSQKVKLPVPVKVQKVNADGKLVEVDTEEITL
                     YKTLRLEYLVGGSAASRTNARVEFLGKEWVMR"
     gene            588..821
                     /gene="rpmA"
                     /locus_tag="PLK08_05490"
     CDS             588..821
                     /gene="rpmA"
                     /locus_tag="PLK08_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018143362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPS52987.1"
                     /translation="MAHKKGQGSTHNGRDSNAQYRGIKCFGGEDVTAGTIIVRQCGTP
                     FHAGRNVGTGKDYTLFAMTSGKVQFRGRYVDVI"
     gene            1179..2213
                     /gene="obgE"
                     /locus_tag="PLK08_05495"
     CDS             1179..2213
                     /gene="obgE"
                     /locus_tag="PLK08_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPS52988.1"
                     /translation="MIHNEHTVSRAIFVDEVDVEVRAGDGGNGCMSFRREKFVPKGGP
                     NGGDGGNGGSVIFRADESLNTLHHLVGHHLYKARRGAHGLGKNCHGKSGDNLYIDVPP
                     GTLVYDAEHDFLLFDLTYNTQEVVIAKGGRGGRGNARFATPENQAPREFEMGTPGEHR
                     KLRLELKLMADVGLVGLPNAGKSTLLSRLSSARPKIGAYPFTTLKPSLGLVELAGFRR
                     FVMADIPGLIEGAHEGAGLGDEFLRHIERTAIILHMVDLYPVDETDPVEDFFIINREL
                     AAYSRKLAEKPQIVVVNKMDLAPENDDRLAVFSRAVGQEVLAISAVTGQGLDALCEKI
                     WNKLHPEPGE"
     gene            2217..3014
                     /locus_tag="PLK08_05500"
     CDS             2217..3014
                     /locus_tag="PLK08_05500"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPS52989.1"
                     /translation="MATDDTDNEKIPSILACDCGNSSIKFAHVKGESVCEVRKLRLGE
                     LAAGLGAELLTIWEQMPKPKRVVACSVNPIALKAIEAAAAESLNEKILVVGRDLELPL
                     DTAVEEPAKLGPDRVCAASAAFDRIGMACVVVDCGSAITVDCVDDAGVFRGGAILPGL
                     AMSAKALTDGTAMLPHVELENPTWVFGCNTSQAIVGGVVCGARGAVRYFIEAYATQLG
                     SWPVVIATGGDAELVFGDTTSGELVQAVVPDLTIRGVAMAYYRSLLK"
     gene            3011..4144
                     /locus_tag="PLK08_05505"
     CDS             3011..4144
                     /locus_tag="PLK08_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPS52990.1"
                     /translation="MSTISPTSACMLTPAGLGGIAVIRLRGGMARKMADEVFSNAAGV
                     APGELRFGKIISAGKVVDEAILACVNDDEFEINIHGGSAVGRLALETLAGCGCRISQH
                     DDAAVEVRPAIFSRHPSFDNSAIGAEMLAILPRICGEFALGVITSQWSAGLSELLSGE
                     FSAAELRAAAERFDIVRRLTDWPEVVIAGCPNAGKSTLANTLVGRNVSIVHPMAGTTR
                     DWVRERAMILGRPVWLTDTAGLWDAAGLHDVDSQAVSRAWQQIRQADLVLLAGCGGAD
                     IELPAEIPSARVLKIFTKADEFTPTRDDILSVSALTGQGIVELEKQILNRLGLDKIFP
                     GQPAAFTERQKKLLLKSAAALDAGQPSLCRQIITEIMRQTCCD"
     gene            complement(4171..4743)
                     /locus_tag="PLK08_05510"
     CDS             complement(4171..4743)
                     /locus_tag="PLK08_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPS52991.1"
                     /translation="MIAELVKKNRSYRRFAQNSAISHQTLVELVDLARNSASAANLQP
                     LKYFISCDPATNEKIFPTMFWAGYLKDWDGPDDGERPAGYIVLLGDTTISQKCDWDAG
                     IACQSILLGAVEKGFGGCILGSINRNKLREVLRIPDYLEIVLIIALGKPVESVTLETA
                     QDGQIKYYRDDSATHHVPKRPLEEILWQPE"
     gene            5161..>5742
                     /locus_tag="PLK08_05515"
     CDS             5161..>5742
                     /locus_tag="PLK08_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HPS52992.1"
                     /translation="MNRKAQPVQAGWDCEEQKLVGGSPAFKQLLAVVDSVACNNCIVL
                     LEGESGTGKELIAQRIHEKSARAKHPFIPVNCPAVSETLFESQFYGHVKGSFTGAASD
                     TLGMVRAADGGTLFLDEIGELPIHLQPKLLRLLQEKEVTPVGDTKTYKVDVRFVAATN
                     RSLAKAVADGRFRRDLYHRLNVVRVHVPPLRQRP"
BASE COUNT         1390 a         1363 c         1608 g         1381 t
ORIGIN      
        1 ccggccagga aataaaagcc gacgaaggca taaatccggc tgtattcagc cagattaaaa
       61 aaactctcgg caatgacctt ctgctggaca aatcacggat gtcgggcaag ctgctgcaag
      121 gcgaagataa cgccaaggaa ggtttggcca tttttaagga tttcgacccg caggctggca
      181 gttttgacat atttatcggc ggcttgtgca gcaattcgca aaaagtcaag ctgcccgtgc
      241 ccgtcaaagt gcagaaagtc aatgccgacg gcaaactcgt ggaagtagat acggaagaaa
      301 taaccctcta caaaactctg cggctggaat acctggtcgg cggttctgcc gccagccgga
      361 cgaacgccag agtggaattt ctcggcaaag aatgggtgat gcgctgattt gtgatttttg
      421 tcgcaaaacg ggaatcacta ttcttgggtt aaaattgaaa tagactgttg acaaacagca
      481 tgaaaagtgt attattaccg ctcctacgcc gcttttgcac aagaataaga tgaattccgc
      541 acggcggctt gaatgatgat aagtagtcag atgcaaggag tttagcaatg gcacataaaa
      601 aaggacaagg ttcgacccat aacgggcgcg acagcaatgc gcaataccgc ggcatcaagt
      661 gcttcggcgg tgaagatgta acagccggca cgattatagt gcggcagtgc ggcacgcctt
      721 tccacgcagg ccgtaatgtc ggcacaggca aggattatac gctctttgcg atgaccagcg
      781 gcaaggttca gttccgtggc cgctatgtag atgtgatatg acaaccgcaa tatgattttt
      841 tacgggatgg cggtattttt ccgccatctt tttttttatc tggataatat catgggatat
      901 ttgtgaaccg gattagagcg gttcttaaaa attttatatt tggtgtaatg ctgagcatgg
      961 cgaagtgtct gaactactac ggtgaaagtg gcaagattcg agaaacaaga ttcaaaaaat
     1021 atgagtaagt tccaattttc aagtatcgta taactgacgg ttatcataaa aaacttggaa
     1081 tttgtttttt gacgttactt gtaagttgtt ttcctgtttc ttgaaaatta ttgttttttc
     1141 ggttgttgcc ggaagcggtt ttaggtagcg ctaaaattat gatacataat gaacatacgg
     1201 tatcgcgtgc catatttgtc gacgaagtgg acgtggaggt tcgagccggc gacggcggca
     1261 atggctgcat gtcgtttcgt cgcgagaaat ttgtgcccaa aggtgggcct aacggcggcg
     1321 acggcggtaa cggcggcagt gtgatattcc gagccgacga atcgctaaat acgcttcatc
     1381 accttgtcgg tcatcatctt tacaaggctc ggcggggcgc acatggcctg ggcaagaatt
     1441 gccacggtaa aagcggcgat aatctttata tcgacgtgcc gcccggcacg cttgtctatg
     1501 acgccgagca cgattttctg cttttcgatt tgacgtacaa tactcaggaa gtggttatcg
     1561 ccaagggcgg cagaggcggc aggggcaatg cgcggttcgc gacgcctgag aaccaggcgc
     1621 ctcgcgaatt tgagatgggt actcctggcg agcatcgcaa gttgcgtctg gagttgaaat
     1681 tgatggctga cgtcggcctt gtaggtttgc cgaacgccgg caaaagtacg ctgctcagcc
     1741 gtctgagctc cgcgcggccg aaaatcggtg catatccgtt tacgacacta aaaccatcgc
     1801 ttggtttggt ggagttggcg ggttttcgca gattcgtgat ggctgacatt cccggcctta
     1861 tagaaggcgc gcatgaaggc gccggcctgg gtgacgaatt tttacgacat atagaacgga
     1921 cagccattat tctgcacatg gtcgaccttt atccggttga cgaaaccgac cctgttgaag
     1981 atttttttat catcaatcgc gagctggctg cgtattcccg caaacttgcc gaaaaacctc
     2041 aaattgtcgt agtcaacaag atggatcttg caccggagaa tgatgacagg cttgccgtat
     2101 tcagccgcgc agtcggccag gaagtgttgg ccatttccgc cgttaccggc cagggccttg
     2161 acgcgctctg cgaaaaaata tggaacaaac ttcatcctga accaggagaa tgacctatgg
     2221 ccaccgacga tacagataat gaaaaaattc cgtcaatcct tgcgtgcgat tgcggcaata
     2281 gctctataaa atttgctcac gtcaagggcg agagcgtttg tgaggttcgt aaattgcggc
     2341 tgggcgagct cgccgccggc ctcggagcgg agctgctgac aatttgggaa cagatgccca
     2401 aacccaaacg tgtcgtggcg tgcagcgtga atccaatcgc cctcaaagcc atcgaagccg
     2461 ccgctgcgga atcgctgaat gaaaaaatcc ttgtcgtcgg ccgtgatttg gaactgccgc
     2521 tcgatacggc tgtcgaagag ccggctaaac tcggccccga ccgcgtctgt gccgcgtcgg
     2581 ctgcatttga ccgcataggg atggcttgcg tggttgtgga ttgcggctcg gcgataactg
     2641 tggactgcgt tgacgatgcg ggcgtgtttc gcggcggagc gattctgcca ggcctggcca
     2701 tgtcagccaa ggcgcttact gacggcacgg cgatgctgcc gcacgttgag ctggaaaatc
     2761 cgacatgggt tttcggctgc aatacttcgc aggcgatagt tggcggcgtg gtctgcggag
     2821 cacgcggcgc ggtgagatat tttatcgagg catatgccac acagctcggc agttggccgg
     2881 ttgtgatagc taccggcggc gacgctgaac tggttttcgg cgatacgacg tccggcgaac
     2941 tcgtacaagc cgtcgtgccg gaccttacta ttcgcggcgt ggcgatggcg tattatcgaa
     3001 gtctgctcaa atgagcacaa tttcgccaac cagtgcgtgc atgctgacgc cggccggcct
     3061 gggcggaata gccgtaatcc gcctgcgcgg cggaatggca cgtaaaatgg ctgacgaagt
     3121 tttttcaaat gcagccggcg ttgcaccagg ggaactgcga ttcggcaaaa taatctcagc
     3181 cggcaaggtc gtggatgagg caatacttgc ctgcgtaaac gacgatgaat tcgaaatcaa
     3241 cattcatggc ggctcagcgg tggggcggct ggcgcttgag acgctggccg gttgcggatg
     3301 ccgcatatcg cagcatgatg atgcggcagt tgaagtcagg ccggcaattt tctccaggca
     3361 tccatctttt gacaattcgg ctatcggtgc cgaaatgctg gcaattctgc cgcgtatttg
     3421 cggcgaattt gcattgggag tgataacttc gcagtggtca gccggcttga gtgaattgtt
     3481 gtcaggcgag ttttccgccg ctgaattgag ggctgcggct gagcggtttg atattgtcag
     3541 gcggctgacg gattggccgg aggtcgtaat cgccggctgt cctaacgccg gcaaaagtac
     3601 gctggctaat acgttagttg gacggaatgt gagcattgtt cacccgatgg ccggcacgac
     3661 gcgggattgg gtaagagagc gagcgatgat tttgggcaga cctgtctggc tgaccgatac
     3721 ggcagggctg tgggatgcgg caggattgca cgatgtcgac tcgcaggctg tcagccgtgc
     3781 atggcagcag attcgacagg cggaccttgt gctgctcgcc ggctgcggcg gagctgatat
     3841 cgaactgccg gctgaaatac cgtctgcccg tgttctgaag atttttacaa aagccgacga
     3901 attcaccccc acacgcgacg atatattatc cgtcagtgcc ttgaccggcc agggcattgt
     3961 tgaactcgaa aagcagattc tcaacagact tggtctggat aaaatttttc ccggccagcc
     4021 ggcggcattt accgaacgtc aaaaaaaatt gctcctgaaa tcagccgccg cactcgacgc
     4081 cggccaacca tcgctttgca gacaaattat aactgaaatc atgcgacaga cttgctgcga
     4141 ctgattcgcc gatattgacg gatgccgtta ttattccggc tgccagagaa tttcctcaag
     4201 cggacgtttt ggaacgtggt gtgtggcact gtcgtcgcga taatatttta tctggccgtc
     4261 ctgagccgtt tccagcgtta cggattccac cggcttgccg agcgcgatta ttaaaactat
     4321 ctccagataa tctggtattc tcaaaacttc ccttaatttg ttgcgattga tgctgcccaa
     4381 aatacagccg ccaaaacctt tttcgaccgc cccaagcaaa atgctctgac aggcaatgcc
     4441 ggcgtcccag tcgcattttt gggaaattgt cgtatcgcct aacaatacga tataccccgc
     4501 aggacgttcg ccgtcgtccg gcccgtccca atccttgaga tagccggccc agaacattgt
     4561 aggaaaaatt ttctcattgg tggctgggtc gcatgatatg aaatacttca gcggctgaag
     4621 gttggcggcg ctggcggagt tgcgtgccaa atccacaagc tctacaagcg tttgatgcga
     4681 aatggccgag ttctgggcaa aacgtctgta actgcgattc tttttgacaa gttcagctat
     4741 catgtggatt ctccttggat ttcaaaaaca aatctaccgg aaaaatcgaa aattaaaaat
     4801 caaaaatcaa aacaaaatac aaaaaccgtt gtaaagaaaa acttttatca tacgaaagcc
     4861 atatttgtaa tggttttttt gatatttttt aaggtataga cctgacaaaa agactggccc
     4921 ggagaaaaaa tttctgtttg gcctaaaaaa gccttgccag atttgatata ggtgttatac
     4981 tgacttatgc ttaattagga agtccccgct cagggactca ccacttggct cgattcattg
     5041 aagtgttgac aagacatgcg tagcgtttgc gcatggtgga tgtgcacatg gtgtacagac
     5101 atatttttgc agtagctcaa ctgcagaaag gctatttaaa tgacagctgg aggaggcgtc
     5161 atgaacagaa aggcccagcc ggttcaggct gggtgggact gcgaagagca gaagctcgtg
     5221 ggcggttcgc ctgcgttcaa acagcttctt gccgtcgtgg actcggtcgc atgcaacaac
     5281 tgtatagtgt tgctggaagg cgagtccggc acgggtaagg agttgatagc gcagcgaatc
     5341 cacgaaaaat cagctcgtgc gaaacatccc tttattcccg tcaactgtcc cgcggtgagc
     5401 gaaacccttt ttgaaagcca gttttatggc catgtgaaag gttcgtttac cggtgcagcc
     5461 agtgacacgt tgggtatggt tcgcgcggcg gacggcggta cgcttttcct tgatgaaatt
     5521 ggcgaattgc caattcatct acagccaaaa ttgctcaggc tgttgcagga aaaggaagtc
     5581 acgccggttg gtgatacgaa aacctacaag gttgacgtgc gatttgtggc ggctaccaac
     5641 cgttcgctcg caaaagcggt tgcggacggc cgattccgcc gcgatctgta tcaccggctg
     5701 aacgtggttc gcgtacatgt cccgccgttg cggcagcggc cg
//
LOCUS       DAONFI010000134         5688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16652_length_5688_cov_4.218356, whole genome shotgun sequence.
ACCESSION   DAONFI010000134 DAONFI010000000
VERSION     DAONFI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16652_length_5688_cov_4.218356"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLK08_05520"
     CDS             <1..487
                     /locus_tag="PLK08_05520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPS52993.1"
                     /translation="RGPFHVVVYKSKRVADLYLQDLFLRRVKVAVGLPETPTPEGFFR
                     VVPWNGKTANSTYYPPADSGKACIPIMPGQPGYPLGPNGYNIKIEGIPQLGTHIEASQ
                     SYALHGTCNPSSLGTQASRGCVRFSDRDIEFLFIALMDYADPKDSRANWPHWSTITIL
                     P"
     gene            527..2128
                     /locus_tag="PLK08_05525"
     CDS             527..2128
                     /locus_tag="PLK08_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPS52994.1"
                     /translation="MAKKFVRISLAVKFRLFFGLALILAISAALGILWLFVEGLSEQS
                     LESPTREITRLRLNEFRRLHPEDPNKASDANSAVIALYRVDDSGVLLTGPSFVPLVEP
                     LNKEDFTYTQRSVIKEFQKKPAKDIIIQQSEDDQGNTIFRCFRAVRNTSSCNECHAKN
                     PTLSNKPIFRPNQLVGMIEMTMPESAASEFLGWLTRGAFVAGGTLAGLLAMVMFAIIS
                     HRIVFRPVHSLRELADKVAEGDLSVRSDVKTGDELERLGESFNEMLGAIQDQHNKLRS
                     ANRALDQKLFELSEANSALFQANIVKSEFLANVSHELRTPLNSIIGFAELLGENADEK
                     ISRYGRNIAVAAKNLLAMINDMLDLARIEAGKARVRADKVSVLDTCQTLLNLMRPLSD
                     KSSLELIGELDEKMPFAVTDGSKLQQILYNLLSNAIKFTPAGGKVILRAYFSEIDQIF
                     TISVIDTGPGISQDDQEHVFEKFYQAEPTLTKEVQGTGLGLAISKELAELLKAKIILK
                     STPGKGTTFSLILPLELKQEEESTK"
     gene            2125..3246
                     /locus_tag="PLK08_05530"
     CDS             2125..3246
                     /locus_tag="PLK08_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS52995.1"
                     /translation="MNVRPIISGIIGAAAGLLLLWAIHAIFFQKAQKATDAVESSAKS
                     SAVQENQFDSDDNIIELAPVTTSSPAAVHETVVKTVSPTSEPASAPVFLTPQDPLTRA
                     KQIHNAQEAILAGDRASSSGDRDFAVACFRRATELDPTNMTALEALAIALLANDEVEQ
                     TVAVYEKIIKLNPADRSAVFNLAMAYIRLKRYENAEEIYNDILNSQPNDIKARTNLAT
                     LLQIQNRPGEARQQWMKVTDIDSTRQDAWEHIGEISVDMGEPAAAMDAYSQAARLSPA
                     DPTVWKNFAITARQAGSGGRAVFAYKKALELEPENSTLWKDLGNVFLEVYRVKKDRDL
                     ITQAVGAWKESIKLNPNQDDVKKLLEIYDTAPTSASTEK"
     gene            3410..4057
                     /locus_tag="PLK08_05535"
     CDS             3410..4057
                     /locus_tag="PLK08_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPS52996.1"
                     /translation="MHELTFYIKAFITLLVLVNPLEGIPVFLAGTERVEPSMRQAISR
                     RTAIAVTIILLISLFLGKALLAMFSISSSAFQIGGGAILFLIAVQMILGPGGGATFSK
                     MAGGEVNLQFAIVPLAIPILAGPGAINGAVLYGTRTTGLWQMLLLAGVIVLVGVGAYA
                     CLRAAEPLAKYLKDSGISIATRIMGLIIAAISVEMILQGIASVFGLKLLEQVSSM"
     gene            4170..5588
                     /gene="atpD"
                     /locus_tag="PLK08_05540"
     CDS             4170..5588
                     /gene="atpD"
                     /locus_tag="PLK08_05540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008729868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPS52997.1"
                     /translation="MTQGRIVQVIGSTFDVEFEESQMPSIFNALRVQIGGQGDLVGEV
                     QQHLGGGRVRCVALGSTDGLKRGLSVEDTGKPVSMPVGPGVLGRVFNLLGEPVDGLGP
                     VKADEYRSIHAPAPEFDKLVPKSEIFETGIKVIDLLAPFVRGGKTGLFGGAGLGKTVI
                     IQELIARVATKHGGYSVFAGVGERTREGNDIWLEMQEAQIGLTGQKLIEKTAMVFGQM
                     NEPPGARLRVALSALTLAEYFRDMGGADTLLFIDNIFRFSQAGSEVSALLGRMPSAVG
                     YQPTLATEMGELQERITSTVKGAITSVQAVYVPADDMTDPAPATAITHLDAFIVLERS
                     IVEKGIYPAIDPLASSSRILDPQTVGTEHYETARRVQQILQRYRELQDIIAILGVDEL
                     SEEDKCTVARARRIERFLSQPFFVAEVFTGKPGNFTPLSETIRSFREICDGKWDHLPE
                     QAFMYVGTVEEAAKQAEQIAIN"
BASE COUNT         1382 a         1471 c         1581 g         1254 t
ORIGIN      
        1 caggggcccc ttccatgtcg tggtgtacaa atccaaacgt gtcgccgacc tttatcttca
       61 ggatttattc ctgcggcggg taaaggtggc cgtagggctg cccgaaacgc caacgccgga
      121 aggttttttc cgtgttgtgc cgtggaacgg caagacggcg aattccacct attatccgcc
      181 ggctgattcc ggcaaggcgt gcataccaat aatgcccggt cagcctggtt atccgctcgg
      241 cccgaacggg tataatataa aaatcgaagg cataccgcag ctgggcacgc atatagaggc
      301 gtcgcagtca tatgcgttgc acggaacgtg taatccgtcg tcgctcggca cgcaggctag
      361 ccggggctgc gtgagatttt ccgacaggga catagaattc ctgttcatcg cgctgatgga
      421 ctacgccgac ccgaaggaca gccgcgcaaa ctggccgcac tggagtacga taaccattct
      481 gccgtgacgg cgacagagtc atgcaacata aataaacgag aatcggatgg caaagaaatt
      541 cgttcgcata tcgctggcag tgaagtttcg cctgttcttc gggctggcgc tgattctggc
      601 tatttcagcg gcgcttggca tattgtggct gttcgtagaa ggcttgagcg agcagagcct
      661 tgaaagtccg acccgcgaaa ttacgcgtct gcggcttaac gagtttcgca ggcttcatcc
      721 ggaagatccg aacaaggcgt ccgatgccaa cagtgccgtt attgcgctct atcgcgtgga
      781 tgattccggc gttctgctta cggggccgtc gttcgtgccg ctggtagaac cgctgaacaa
      841 ggaagacttc acatacaccc agcgcagcgt cataaaggaa tttcaaaaaa aaccggcgaa
      901 ggacataata atccaacagt ccgaggatga tcagggcaat acaatctttc gctgtttcag
      961 ggctgtgcgt aacacgtcgt catgcaatga atgccacgca aaaaatccga ccttgtcgaa
     1021 caagcccata ttccggccca atcagctcgt cggcatgatt gagatgacca tgcccgaatc
     1081 tgcggcgagt gaatttctcg gctggctgac gcgaggtgcg ttcgttgcag gcggcacgct
     1141 ggccggcctg cttgccatgg tgatgttcgc cataatctca cacaggatag tatttcggcc
     1201 ggttcactct ctgagagaac ttgccgacaa agtcgccgaa ggcgacctta gcgtacgcag
     1261 cgacgtgaaa accggcgacg aacttgagcg gctaggcgag agttttaatg aaatgctcgg
     1321 cgctatccag gaccagcaca acaagctgcg ttccgccaac cgcgccctgg accagaaact
     1381 tttcgaactc agcgaggcga attccgcact gtttcaggcg aacatcgtca aaagtgaatt
     1441 tctggctaac gtttcgcatg aacttcgtac gccgctaaac agcattatcg gctttgccga
     1501 actgcttggc gaaaatgccg acgagaagat atcacgttac ggcagaaata ttgctgtggc
     1561 ggcaaagaac ctgctggcga tgataaacga catgttggac ctggctcgca tagaagccgg
     1621 caaagcccgc gtccgtgccg acaaggtctc cgtcctcgac acctgccaga cgctgctaaa
     1681 tctcatgcgt ccgctaagcg acaagagcag ccttgaactt atcggcgagc tggacgaaaa
     1741 aatgcccttc gccgtaaccg acggcagtaa acttcagcag atactctaca acctgttgag
     1801 caacgccata aaattcaccc cagccggcgg caaggtgatt cttcgcgcat acttcagcga
     1861 gattgaccag atttttacca taagtgtaat cgacaccggc ccgggcatca gccaggacga
     1921 ccaggaacat gtttttgaga aattttatca ggcagagccg acgcttacca aggaagtcca
     1981 gggtacgggc cttggccttg ccatatccaa agaactggcg gaactgctca aggcgaaaat
     2041 catactaaaa agtacgcccg gcaaaggtac gacgttctcg ctcatcctgc cgcttgaact
     2101 taaacaggaa gaagaatcca ccaaatgaat gtgcgaccaa taatttccgg aataatcggt
     2161 gcagcagccg gcttgctgct gctgtgggct atccacgcga tattttttca aaaagcgcaa
     2221 aaggcgacgg atgccgtcga atcgtcagcc aaatcttcgg ctgtgcagga aaatcagttt
     2281 gattccgatg acaatataat agaactcgca cctgtgacga catcgtcgcc ggcagctgta
     2341 catgaaacag ttgttaaaac agtttcgccg acgtctgagc ctgcgtctgc gccggttttc
     2401 ctgactccgc aggacccgct tacgcgggca aaacagattc ataacgcgca ggaagcgatt
     2461 ttagccggcg acagggcgtc ttcttccggc gacagggatt tcgcagtcgc ctgttttcgt
     2521 cgtgcaacag aacttgaccc gacaaatatg accgctcttg aggcgttggc tatcgcactg
     2581 ctggcgaacg acgaagttga acagaccgtc gccgtttatg aaaaaataat caagctgaac
     2641 ccagccgaca gaagtgcagt atttaatctt gccatggcgt acatacgcct gaaacgatat
     2701 gaaaacgccg aggaaattta taatgatatc ctcaacagtc agcctaacga cataaaagcc
     2761 cgtacgaacc tcgcgacgct tctgcagatt cagaaccgtc ccggcgaagc ccgccagcaa
     2821 tggatgaaag tgacggatat cgactcgacc cgtcaggatg cctgggaaca tatcggcgaa
     2881 atttcggtag atatgggtga gccggctgcc gctatggacg cctattcgca ggcggccaga
     2941 ctatcgccgg ctgacccgac ggtatggaaa aattttgcaa taaccgcccg gcaggccggc
     3001 agcggcggca gagctgtctt cgcatataaa aaagcccttg aactcgagcc ggaaaacagt
     3061 acgctctgga aagatttggg caatgtcttt ttggaagtct atcgagtcaa aaaagaccgc
     3121 gaccttatca cgcaggctgt cggcgcgtgg aaagaaagca ttaaactcaa tcccaatcag
     3181 gacgacgtca aaaaacttct cgaaatttac gatactgccc cgacgtctgc gtcgacggag
     3241 aagtaatgac attccggcct tgttttgaat ttgcgtcaga gtataattgc agtctgcata
     3301 tgtaggcgtt ttagagtggc tctcaaaatg aaaagtgtaa gaattttgag aattgctctg
     3361 gagcctgctt taatcgtaaa aagctttctg gaactgtgat aagaattcca tgcatgaact
     3421 gacattttac ataaaagcgt tcattaccct gctggtgctg gtcaatcctc tggaaggcat
     3481 tccggtgttt ttagccggca cggaacgtgt agagccgtcc atgcggcagg cgatttcccg
     3541 ccgtacggca attgccgtga cgattattct gttgatatct ttatttctgg gcaaggcgct
     3601 gcttgcgatg ttcagcataa gttccagcgc gttccagata ggcggcggcg cgattctgtt
     3661 cctgatagcc gtacagatga tactcggccc tggcggcggt gcgacgtttt caaaaatggc
     3721 tggcggagaa gtcaatctgc agtttgccat cgtaccgctg gcgataccga tactcgccgg
     3781 ccctggcgcg ataaacggcg cggtgcttta tggaacgcga acgactggcc tgtggcagat
     3841 gctgctgctg gctggcgtga tagtgctggt tggcgttggg gcgtatgcct gtctgcgggc
     3901 ggctgagccg cttgcgaaat atttgaagga cagcggcata agcatagcca cgcgaataat
     3961 gggccttata atagcagcca tatcggtcga aatgatattg cagggcatag cttcggtttt
     4021 cggccttaaa ttgttggagc aggtttcatc catgtagata aagacggctg atggtgtaaa
     4081 accgtttttg cggctagccg ttgtcggcat cgggcgcgat ggcagtttta ttagaaaaat
     4141 tagaagaatt agaagaacag gacggattaa tgacgcaggg cagaatagta caggttatcg
     4201 gctcgacgtt cgacgtcgaa tttgaagaat cgcagatgcc gtcgattttc aatgccttgc
     4261 gtgtgcagat aggcggccag ggcgatctgg ttggagaagt tcagcagcat cttggcggcg
     4321 gccgggtacg gtgcgttgcg ctgggttcta cggacggcct caaacgcggt ctttccgtcg
     4381 aagataccgg caagccggtg tccatgccgg tcggccctgg tgtgttgggc agagtattca
     4441 atctgctggg cgagccggtg gatgggcttg gcccggtaaa ggctgatgag tatcgctcga
     4501 tacatgcccc tgcgccggaa ttcgacaaac ttgtccccaa aagcgagata ttcgagaccg
     4561 gcataaaagt gatagattta ctggcgccgt tcgtccgcgg cggcaagacg ggccttttcg
     4621 gcggcgccgg cttgggcaag acggtcataa tccaggaact cattgcccgc gtagctacga
     4681 aacacggcgg atattcggtc ttcgccggcg tcggcgaacg cactcgcgaa ggcaatgaca
     4741 tatggctgga aatgcaggag gctcagatag gccttaccgg ccagaaactt atcgaaaaaa
     4801 ccgcgatggt cttcggccag atgaacgaac cgcctggagc ccgcctgaga gtggcgctca
     4861 gtgcgttgac gctggctgaa tattttcgcg atatgggcgg cgccgatacg ctgctgttca
     4921 tagataacat tttccgtttc agccaggctg gcagcgaggt ttccgcactg ctgggccgta
     4981 tgccctccgc cgtgggttat cagccgacgc tggctaccga aatgggcgag ttgcaggaac
     5041 gaatcacgtc gaccgtcaag ggtgcgataa cgtccgttca ggcggtttac gttccggctg
     5101 acgatatgac cgaccctgcg ccggcgacgg ctatcacgca tcttgacgca ttcatcgtgc
     5161 tggaacggag cattgtggaa aagggcatct atcccgccat tgacccgctg gccagttcca
     5221 gccgcattct tgacccgcaa accgtcggca cggaacatta cgaaaccgcc cgccgcgtgc
     5281 agcagattct tcaacgctat cgtgaattgc aggatatcat cgccattctc ggcgtcgatg
     5341 aactaagcga agaagacaaa tgcaccgtcg cccgtgcgcg aaggatagag agattcctct
     5401 cgcagccgtt tttcgtggcg gaagttttta cgggaaaacc cggcaatttt acgccgctca
     5461 gcgagaccat ccgcagtttc agggaaatct gcgacggcaa gtgggatcat ctgccggagc
     5521 aggctttcat gtatgtcggc actgtcgaag aagccgcaaa gcaggctgaa cagatagcga
     5581 taaactagag catattaaac ttgactttgc gcttgttgct gtttgcggcg tcagctggct
     5641 gcgtcaggct gcatcggaaa ccatataagg ttgcctgctt cgccttcc
//
LOCUS       DAONFI010000135         5663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16736_length_5663_cov_3.582739, whole genome shotgun sequence.
ACCESSION   DAONFI010000135 DAONFI010000000
VERSION     DAONFI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16736_length_5663_cov_3.582739"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLK08_05545"
     CDS             <1..746
                     /locus_tag="PLK08_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS52998.1"
                     /translation="KMRNAFLARKNGAERVAKSREKQRCNRDVTQGCNGDVTAHIYSY
                     SNNNILPLTPPTQNSGVENSPLKREEAEEDFQGSSRGEEKNNPHVRRLAEIYREAYGH
                     EMPGTWKNAIAGETDAGLLEKITAKDFRTAMEKFPQSGMKIWAWGCVRYYAQQRLARA
                     ASAAKPAEVGVYPTKPPEAKSDDSRELLERFRREVPESERRRLIAEQHAKFKFMPKAA
                     LERTTAYEWAQPAMKESQKQGIKSCQNTK"
     gene            728..1855
                     /locus_tag="PLK08_05550"
     CDS             728..1855
                     /locus_tag="PLK08_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HPS52999.1"
                     /translation="MSEHKIGWLNVPGYKGETWNPIIGCSKCSPGCQHCYAERMAGRL
                     VNIKQTEYYSGVVNHLVENSYGRVVDTVWNGKTAFVESALEKPLHWKQPRVIFVCSMS
                     DLFHETVPFEWIDQVFAIMARCPQHKFLLLTKRPQRMVEYFANIGGTSRHDWVWTCMA
                     RQMNVSHLKGINWPLPNVGIGVTICNQEEADAKIPMLLSIPAAMRFVSIEPMLGKIVL
                     QTDTKYCETCGLHFNPIEIESEIEHRDGSKTIEVETFCERCHEIEPEMMSTVCGCHIG
                     LDWIIVGGESGPKARPMHPDWVRNIQQQCSDSGVPFFFKQNGEWLNSDKFSEGEIIAA
                     IVCNRWYVFDDGIGMIQCGKQRAGHLLDGLEYREYPKWIER"
     gene            1859..2119
                     /locus_tag="PLK08_05555"
     CDS             1859..2119
                     /locus_tag="PLK08_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53000.1"
                     /translation="MITKITLQNDRLIKISKLKRGDKFIVDWATRDSEKIFVCAGVTD
                     LDPSCGLITAICMTTGEIACFHGYETVSLVEITDITITGIVR"
     gene            2123..2458
                     /locus_tag="PLK08_05560"
     CDS             2123..2458
                     /locus_tag="PLK08_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53001.1"
                     /translation="MQTNREELEEYKARFKVAHQLVNGLQRQIDSAKRSRSALKGQLT
                     RERKRNAEAKELFELLEWAEGFTRLTYRHETGDWSIVFILDSISNCIINKSLLLALRE
                     AKKNIETQK"
     gene            2470..2901
                     /locus_tag="PLK08_05565"
     CDS             2470..2901
                     /locus_tag="PLK08_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53002.1"
                     /translation="MKHKMTQKEFDVLEIINGVKNCPANTDYSLISSFGEGCRFGEGC
                     RFGEAKYEAIGSTIYYAGGFGSCNRTTYGIPTKDGVFVRCGCWSGWLSEFRNRIHSVY
                     DGYDIEAEYMIIANLFEARWMRQMEALDLEVEAKKIVEGEK"
     gene            2898..3503
                     /locus_tag="PLK08_05570"
     CDS             2898..3503
                     /locus_tag="PLK08_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HPS53003.1"
                     /translation="MKVLVACEFSGRVRDAFIKRGHDAMSCDLLPTEQPGPHYQGDVF
                     DIINDGWDMMIAHPPCTWLCQAMRTNATRKDRPLITAQFPAKRQEAFEFVMKLSEANI
                     AHIAIENPIGYLNGNWRRPDQIIRPWMFGHGYRKDVCLWLKNLPMLKSTNIVDGRKRL
                     DFWSNKRNPDGKSLKSITFQGIADAMAEQWGNLPTESDGAK"
     gene            3500..4273
                     /locus_tag="PLK08_05575"
     CDS             3500..4273
                     /locus_tag="PLK08_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53004.1"
                     /translation="MSQIKTNGLFCDTECPFFRDDHEGTASCFSDNKWKALAYSWSAK
                     ETPRHKSCVLSELAEKDREIERLSKRDGNASIELCYDDGFNPFARSDCKHLDFGVADN
                     CYVIESELLNETLRENKTLRDMCEKMAGTLEKLRVEFLHGPVPQPNHICGTPNAQCDC
                     LCQNYATQCDILHEAELVCKTYAAYALPYRAAKPKPRKIQPTHAAIMLTSLIEKGCPI
                     TISDRVIAVIDGEQLYVFGYKMSVFIVNITEMESSTMPV"
     gene            4464..4607
                     /locus_tag="PLK08_05580"
     CDS             4464..4607
                     /locus_tag="PLK08_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53005.1"
                     /translation="MNSGTSSASYDPMGLVPITIPCTPESISDLEFYAASNTKMTILA
                     TGV"
     gene            4604..5419
                     /locus_tag="PLK08_05585"
     CDS             4604..5419
                     /locus_tag="PLK08_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53006.1"
                     /translation="MMRVVDGIIQLGNNKLDAFYDPFSIPAAIAYLFNNSGKIQIGSD
                     WYLRNFGTLGTDWRLKAYNAVIDGNIPTENITVGTAQYAGCIGDVATSRRFVTDAVMP
                     CALGYGSYTFELIWYDRGGRGDLESAQTSQSYYLGSGSSLWYIWHNGGTSNLCVSSAN
                     GCSDSGWTNGYWSGTGARHLLVSLNATTGLLTRCLNGTVTMASGQSPVTNTDSIQLSI
                     LGQSSGGDAYSIASASTVCQLSIWQGFAATSAQIATRKLAVYAGTADAVRWEL"
BASE COUNT         1672 a         1149 c         1422 g         1420 t
ORIGIN      
        1 gcaagatgcg aaatgcgttt ttagcccgta aaaatggggc tgaacgcgtg gcaaagtcga
       61 gggaaaaaca gagatgtaac agagatgtaa cacaaggttg taacggagat gtaacggcgc
      121 atatctatag ttatagtaat aataatatcc tcccccttac cccccccacg caaaattccg
      181 gcgtggaaaa ttcgcccttg aagcgggagg aggcagagga ggatttccag gggagcagca
      241 ggggagaaga aaaaaacaat ccgcacgtcc ggcgacttgc cgaaatttat cgcgaggcgt
      301 acgggcacga aatgcctgga acttggaaaa acgcgatagc cggcgaaacc gacgccggcc
      361 tgctcgagaa aattacggcg aaggattttc gtacggcgat ggaaaaattc ccgcagtcgg
      421 gaatgaaaat ctgggcgtgg ggctgcgtgc ggtattacgc ccagcaacgg cttgccaggg
      481 ctgcttccgc ggcaaagccg gccgaagtcg gcgtatatcc gaccaagccg ccggaggcca
      541 agtcagacga ttctcgcgag cttttggagc gatttcgacg ggaagttccg gagtcggaac
      601 gcagacggtt gattgcggag cagcacgcga agtttaaatt catgcccaag gctgcccttg
      661 agcgaaccac ggcgtacgaa tgggcacaac ctgcaatgaa agaatcccaa aaacaaggaa
      721 taaaatcatg tcagaacaca aaataggatg gctgaacgtg ccaggttaca agggcgaaac
      781 atggaatccg ataatcggtt gcagcaaatg ctcgcctgga tgtcagcatt gctatgcgga
      841 gcggatggcg ggacggctgg ttaatataaa gcaaaccgaa tattattcag gcgttgtcaa
      901 tcatcttgtt gaaaattcct acggtcgcgt ggttgatacc gtctggaatg gcaagacagc
      961 ttttgttgaa tctgcccttg aaaaaccact gcactggaaa cagcctcgcg taattttcgt
     1021 ctgctcaatg tcagacctgt tccatgagac agtaccgttt gagtggatag accaagtttt
     1081 cgcaatcatg gcgcgttgtc cacaacacaa gtttttattg ctgacgaagc ggccgcaacg
     1141 catggttgaa tattttgcca atattggtgg aacatctcgc cacgattggg tctggacttg
     1201 catggcccgc cagatgaatg ttagccatct caagggtata aattggcccc tgccaaacgt
     1261 tggtatcggc gtaacaattt gcaatcaaga agaagccgac gctaaaatcc ccatgttgct
     1321 ttcaatcccc gcagctatgc gattcgtttc cattgagccg atgctgggta agatagtatt
     1381 acaaacagac accaagtatt gcgagacctg cggattacat ttcaatccca tcgaaataga
     1441 atcagaaatt gaacatcgcg atggttcaaa aacaatagaa gttgagacat tctgtgagcg
     1501 ttgccatgaa atcgaacccg aaatgatgag tacggtttgc ggttgtcata ttggccttga
     1561 ttggataatc gtaggcggcg agagcgggcc taaagcaagg cctatgcacc ctgattgggt
     1621 gcggaatatc caacaacaat gcagcgattc aggcgtgcca ttttttttca aacagaacgg
     1681 ggaatggttg aactccgata aattcagcga aggcgagatt atagccgcaa tagtatgtaa
     1741 tcgctggtat gtatttgatg atggtattgg aatgattcaa tgtggaaaac aacgcgccgg
     1801 ccatcttctc gacggcctgg aataccgcga gtacccgaaa tggattgaga ggtgaatcat
     1861 gataaccaaa atcacattac aaaacgatag gcttataaaa atttcaaaat tgaaacgcgg
     1921 cgataaattt attgtcgatt gggctaccag agattctgaa aaaatttttg tttgtgcagg
     1981 cgtaacagat ttagacccaa gttgcggtct aataactgct atctgtatga ctacgggcga
     2041 aatcgcatgt ttccacggat acgaaacagt aagtctagtt gagataacag atataactat
     2101 tacaggtatt gtgaggtgaa aaatgcaaac taacagagaa gaacttgagg aatataaggc
     2161 aagattcaag gttgcacacc aactggttaa tggtttgcaa cggcagatag attcagccaa
     2221 gcgttcccgc tccgccctca agggccaact tacccgcgag cgaaagcgca atgccgaagc
     2281 taaagagctg tttgaactgc tggagtgggc cgaaggattt acgagactca catatcggca
     2341 tgaaacaggc gattggtcta tcgtatttat cttagatagt atctcgaatt gtattatcaa
     2401 taaatctctt ctcctcgctc tccgcgaggc aaagaaaaat atcgaaactc aaaaataaag
     2461 gaaagaatta tgaaacacaa aatgactcaa aaagaatttg acgttttaga aattattaat
     2521 ggggtaaaaa attgccctgc aaataccgac tattcgctga ttagcagctt cggcgagggg
     2581 tgccgcttcg gcgaggggtg ccgcttcggc gaggctaaat atgaggctat cggcagtact
     2641 atatattatg caggggggtt tggaagttgt aacaggacta cttatggtat tccgacaaaa
     2701 gatggggtat ttgttcgttg tggttgttgg tctggttggt tgtcggagtt ccgcaatcgg
     2761 atacatagtg tgtatgacgg atatgacatc gaagctgaat acatgataat tgcgaatcta
     2821 tttgaggcta gatggatgcg tcaaatggaa gcactcgact tagaggttga ggcaaagaag
     2881 attgtggagg gggagaaatg aaagtcttag ttgcatgtga attttcaggc cgcgtccgcg
     2941 atgcttttat aaagcgtgga catgatgcaa tgtcgtgtga cctgctgccg accgaacaac
     3001 ctggcccaca ctatcaggga gatgttttcg acattataaa tgatggttgg gatatgatga
     3061 ttgcacatcc tccatgcacc tggttgtgtc aggctatgcg gacaaacgca accagaaaag
     3121 ataggccatt aataactgca caatttccag caaaaagaca agaggcattt gagtttgtaa
     3181 tgaaactgtc agaggcaaat attgcgcata ttgctatcga gaatccaata ggctacctca
     3241 atggaaactg gagaagacct gaccaaatta taaggccctg gatgttcgga catgggtaca
     3301 gaaaagatgt ttgtttatgg ctcaaaaact tacctatgtt gaaatctact aacatcgtag
     3361 acggcagaaa acgactggat ttttggagca acaagcgtaa tccagatgga aaaagtttga
     3421 agtctattac ttttcaaggc attgccgacg ctatggctga acagtgggga aatcttccca
     3481 cagaaagcga cggtgcgaaa tgagtcagat aaaaacaaat ggattatttt gcgatactga
     3541 atgcccattc tttcgagacg accatgaagg tacagcctct tgcttcagtg ataataaatg
     3601 gaaagctctt gcatattcat ggtcggcaaa agaaacacca agacacaaat cgtgtgtgct
     3661 aagtgagctt gcggaaaaag acagggagat tgaaaggcta agtaagcgag atggtaatgc
     3721 gtctattgag ctttgttatg atgatggatt taatcccttt gcaaggtctg attgcaaaca
     3781 tctcgatttt ggcgttgctg ataactgtta tgttattgaa agcgaactgt taaatgaaac
     3841 acttcgagaa aataaaacgc tccgtgatat gtgcgagaag atggcaggga cgttggaaaa
     3901 actacgtgtt gaatttcttc acgggccagt tccacaacct aaccatattt gtggaacacc
     3961 taatgcacaa tgtgattgcc tctgccaaaa ttatgctacc caatgtgata tattacatga
     4021 agctgaatta gtatgtaaga cctacgccgc atatgctctg ccatatcgag ccgccaaacc
     4081 caaacctcgg aaaatccagc cgactcatgc tgcgataatg ctgacaagtc taattgaaaa
     4141 gggctgcccc attacaatca gcgacagggt tatagccgta attgatggcg aacaactgta
     4201 tgtattcgga tataaaatgt cggtatttat agtaaatatt acggagatgg aatccagtac
     4261 gatgccagtc tgaatatacc cgccttgcga atcgtatcct taaaaacttt attgaggata
     4321 tggaaatgca aaaaccgcag gtattgattg taacggtcag cagcacgagt aaaaccgtcg
     4381 cggaactctt ggaggccgca gggctgactc tgcctgaatg gacgtctgaa gttttacttc
     4441 gtccacacgg tactgggata tatatgaact ctggaacatc atcggcgagc tatgacccga
     4501 tgggcctcgt accgataact attccgtgta cgccggaatc gataagtgac cttgagttct
     4561 acgccgccag caacactaaa atgaccatac ttgcgacggg tgtatgatgc gtgtggtaga
     4621 cggcataatt cagttgggca acaataagct tgatgctttt tacgacccat tcagcattcc
     4681 cgccgctatc gcctatttgt ttaataattc cggtaagata caaattggta gtgattggta
     4741 tttgcggaac tttggtactc ttggaacaga ctggcggcta aaagcttata atgctgttat
     4801 tgatggcaat attccgactg aaaatattac tgttggaact gcacaatatg caggttgcat
     4861 tggcgatgtc gcaacttctc gcagatttgt aaccgatgca gtgatgccct gtgctcttgg
     4921 ttatggctca tatacatttg aattaatatg gtatgaccgt ggggggaggg gcgaccttga
     4981 atccgctcaa acatcccaat cttactatct tggctctggg tcaagcttat ggtatatatg
     5041 gcataatgga ggcacaagca atctttgcgt gtcatctgca aatggatgca gtgatagtgg
     5101 ctggactaat ggttattgga gtggcactgg tgctcggcat ttgttggtat ctctcaatgc
     5161 tactactgga ttattaacac gttgtttaaa cggtactgtc acaatggcca gtggtcaatc
     5221 accagttaca aatacagata gcattcaatt aagtatactt ggacaatctt cgggcggaga
     5281 tgcttatagt attgcttctg ccagtacagt ttgtcagctt tcaatctggc aaggtttcgc
     5341 cgcaacgtct gcgcagatag caacccgcaa acttgcggta tatgcaggta cggcagatgc
     5401 tgtgaggtgg gaattatgat atgggcaata tttgaccata ttccagatgc aacaaaactc
     5461 gacaggccgg cgatgggata ttccccacag cctcatactc ttattggcgg cgccgaaata
     5521 tattgcgtgt gctgtatgag tatcggtagt gaaaccgcga ttgaaatatt taacacaggc
     5581 gttgaggctg aaaattggtt atccacacat tgtaataatg gagacgaggc atgaacggtc
     5641 agtgcaataa aatcaaccct gat
//
LOCUS       DAONFI010000136         5635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16843_length_5635_cov_4.674552, whole genome shotgun sequence.
ACCESSION   DAONFI010000136 DAONFI010000000
VERSION     DAONFI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16843_length_5635_cov_4.674552"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLK08_05590"
     CDS             <1..1107
                     /locus_tag="PLK08_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53007.1"
                     /translation="RLERLVSRRSTGSLTELYADWLGEWLTIPDKCSSPAHERRIRQA
                     SDFFSLDCSPQRLKLAELFINDYERLGNLYNQAVATADVGVHPLDMTAGELPFYLIFR
                     RDGRLVRCECRLQDGRITAGDMEFDASAGLPLVAMKHAGVLCIAGKAILLVLQVRAGQ
                     TGSYLALPYHGSMYMPAAYEFERLLTTEGVLPQKYFKPVLRVRFKLLDRLAKLDESVM
                     INLPTHLAEIFGRDDMPAKEFAAEYNEVIAAARSRLTDFADETRRKSWMKTQFAAEYA
                     EMAVIENRRRELAKTQPKCPEMRSLWENEKKLRVKTLAGWLNRLYLDSQAAAMDFYDS
                     RGAILPWCIALGGEAFYKYVISHAEIYKESPAAT"
     gene            1117..1764
                     /locus_tag="PLK08_05595"
     CDS             1117..1764
                     /locus_tag="PLK08_05595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPS53008.1"
                     /translation="MNDKIDKIRNHYLPRIKSCRKNYDVLDWAAGETQLLRFSILADN
                     VPLNGLTLLDVGCGLGDLAQYLAVRKIALCRYAGVDVLPEMLLRARQDKPELELFAAD
                     IFAMSAHAALSFLKADKPFDVVYCSGAFNLNLGNNESFMQNAVEKMAALASKWLVFNC
                     LHARHKIKDDRYYAYQPTAAMAAVRAACGDAEIHILDKYLENDFTVICRIPDFQP"
     gene            1837..3102
                     /locus_tag="PLK08_05600"
     CDS             1837..3102
                     /locus_tag="PLK08_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53009.1"
                     /translation="MKKLKSTRTFCVILWAVLLATAGEVFCASADTSSDNQDAAPSFE
                     AAAIETLPAGLPEFNKSAADAPKKSQTQPAEPPSIKPATPKNITPAQATNKAEKTLSP
                     SSASSAARPADESGTEEEKLQNIILKLQLENSRLMAALNKGHQVNANLTNALRESRMH
                     ASDMEAEIRRKEIELTVKNEEAVLERLKTVATTQPAKTAQILQQENNILSLQLKLYRI
                     DEPQRTKEMLEKIGDITRERDTLADKNKRIHTMLNEQIKKNEQTGEELKVIRNNFQSS
                     EKEIARLQLEIKAYKEAMANPAAASRVEYVTRKPVTTSPAANKTSSADDESQSASDDE
                     EESSNNNPVKIEINRIAGKITSIDDQIIKINVGSLHGLQAGMRLIVYRRDKFVGYLRI
                     DIVGDTTAAGAMSRQVFPPQIGDNVVDRL"
     gene            3154..4455
                     /gene="eno"
                     /locus_tag="PLK08_05605"
     CDS             3154..4455
                     /gene="eno"
                     /locus_tag="PLK08_05605"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPS53010.1"
                     /translation="MARIADIIGREILDSRGNPTVEVDVYLEDGTRGRAAVPSGASTG
                     ENEACELRDGDPKYYGGKGVLHAVANVNGELRDVVVGMDAEAQKAVDNAMIQADGTKN
                     KSRLGANAILGVSMAVARAQANSMGLELYEYLGDDDSVILPVPMMNILNGGKHADGTV
                     DFQEFMIQPLGAETFRQALQMGAEVFHSLKSVLKNKGYSTGVGDEGGYAPSLKSNEEA
                     PELILTAIEKAGYAPGEDIFIALDPAASELANEARKLGREGYCFFKSNPSRIIDNEEM
                     VDIWVDWCSRYPIMSIEDGCGEEDWDGWKLLTDRLGDKIQLVGDDLFVTNVNFLQKGI
                     DLGVANSILIKVNQIGTLTETLAAINMARRADYTAVVSHRSGETEDTTIADLVVAEKT
                     GQIKTGSASRTDRVCKYNQLLRIEEKLGDKAVYGARMWRQN"
     gene            4427..4990
                     /locus_tag="PLK08_05610"
     CDS             4427..4990
                     /locus_tag="PLK08_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53011.1"
                     /translation="MAQGCGDKIEIVEESGGGAFFRRLGFLVVTGMALTLLAALVLLP
                     AWAEKEKTRYELERDTLRLQEAKETVQALSRMMRDVQNDEILTQRLAGTRLGLYTPDE
                     IIVRDPARPVDNPEILSEIHYPQPSHKDSFWLQTAAKLEKQGKRRSYMLLSAAMLISA
                     FILFAPPTQKHGWRPWKLPPPPITKKD"
     gene            5021..5584
                     /locus_tag="PLK08_05615"
     CDS             5021..5584
                     /locus_tag="PLK08_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53012.1"
                     /translation="MPAWLAHIQSVSDNKEAAMKNLFMTIMCIAAAVGGCKEFDPANY
                     DKIEIQCVSKTIDYRQKVIAVKQGDKLHLTFYDMNSIPSLQFSADYQQDSRFAKKFFK
                     LLNPEKMIKMGDSTQMELTGGRYDYSVKLDNDENAFTLYSPCSLEVVIVPAKALGLMA
                     NTDKLFTELQNEAGRKNPKHNDDAPTP"
BASE COUNT         1563 a         1440 c         1462 g         1170 t
ORIGIN      
        1 aggctggagc gtcttgtttc caggcggtca accggcagcc tgaccgagct ttacgccgac
       61 tggctgggcg aatggctgac tattccggac aaatgctctt cgccggcaca cgagcgaaga
      121 attcggcagg cgagcgattt tttttctctg gactgttcgc cgcaacgtct gaaattggct
      181 gagcttttca taaatgatta tgagcggctc ggcaacctgt ataatcaggc ggttgcaact
      241 gccgatgtgg gcgtacatcc gcttgacatg acagccggcg aattgccgtt ttatctgatt
      301 ttccgcaggg acggccgatt ggttcgctgc gagtgtcggc ttcaggatgg tagaatcacg
      361 gcaggcgaca tggagttcga cgcgtctgcc ggcttgccgc ttgtcgcaat gaagcacgca
      421 ggcgtattgt gcatagcagg caaggcgatt ttactcgtac tgcaggtacg ggcaggccaa
      481 acaggttcgt acctggcgct gccgtatcac ggctcgatgt atatgccggc ggcatatgaa
      541 tttgaacggc tgctgacgac ggaaggagta ttgccccaaa aatatttcaa gccggtcctg
      601 cgagtgcgat tcaaactgct ggacaggctg gcgaaactgg atgagtctgt aatgataaat
      661 ctcccaacgc acctggccga aattttcgga cgcgatgata tgccggcaaa ggaattcgcc
      721 gccgaatata atgaagtgat agctgccgcc cgtagtcggc tgacagattt tgcagatgaa
      781 accaggcgaa aatcatggat gaaaacccaa tttgccgccg agtatgcaga aatggctgtg
      841 attgagaacc gccgtcgcga attggcgaaa acacagccca aatgccctga aatgcgcagc
      901 ttgtgggaga atgaaaaaaa attgcgtgtc aaaacgctgg ccggctggct gaaccgtctg
      961 tacctggatt cacaggcggc ggcgatggat ttttacgatt cacgaggagc gatattgccg
     1021 tggtgtatcg cactcggagg cgaggctttt tataaatacg tcatctccca cgccgaaatt
     1081 tacaaagaaa gtccagccgc cacctgaatt atgaaaatga atgacaaaat agataaaatc
     1141 cgtaatcatt atctgccgcg cataaaatca tgcagaaaaa attacgacgt gctcgactgg
     1201 gctgccggcg agacgcaact gctgcgtttt tcaatattag ccgacaatgt tccgctcaac
     1261 ggcctgacgc tgttggacgt cggctgcggg cttggcgatt tggcccaata tctcgccgtc
     1321 agaaaaattg cgttgtgtcg ctacgcaggc gtcgacgttc tgcccgaaat gcttctccgt
     1381 gcgcgtcaag acaagccgga acttgaactt ttcgcggctg acatttttgc aatgtctgct
     1441 cacgcggcat tgagtttttt aaaggcagac aaaccgttcg acgtagtcta ctgcagcggc
     1501 gcatttaacc tgaatctggg caataacgaa agttttatgc aaaacgccgt cgaaaaaatg
     1561 gccgcactcg ccagcaaatg gctggtgttc aactgcctcc acgcccgtca taaaatcaaa
     1621 gacgaccgat attacgccta tcagccgacc gccgcgatgg cggctgtccg cgccgcatgc
     1681 ggcgacgcag aaatacacat tctggacaaa tacctcgaaa acgacttcac cgtcatatgc
     1741 cgaattccgg attttcagcc ataaaagacg gcggttatgg caataataat aaaaaaatgc
     1801 ttgtagcagg ctgtaaataa attatcatgt cgggttgtga agaagctaaa atcaaccaga
     1861 acgttttgtg taattttgtg ggctgttctg ctagcgacgg ccggcgaggt tttttgcgcc
     1921 tcagccgaca cctcttccga caatcaggac gcagccccgt cgtttgaggc ggcggcaatt
     1981 gaaactcttc cggcaggcct gccggaattc aacaaatcag cggccgatgc gcctaaaaaa
     2041 tcacagactc aaccagctga gccgccgtca ataaaaccgg caacgccaaa aaatataact
     2101 ccggcacagg ctacaaataa ggcggagaaa acgctatcgc cgtcgtcagc ctcttccgcc
     2161 gccaggcccg ccgacgaatc cggaaccgaa gaagaaaaac ttcaaaacat catacttaaa
     2221 cttcagctgg aaaattcacg gctgatggct gcactcaata aaggccatca agtcaatgca
     2281 aacctgacca acgccctgcg cgaatcgaga atgcacgcaa gcgatatgga agcggaaatc
     2341 cgtcgcaagg aaatcgagct tacggtcaaa aacgaagaag ccgtccttga acgtctgaaa
     2401 accgtcgcca caacccagcc ggcgaaaacc gcccaaatcc tccagcagga aaacaatatc
     2461 ctgtcgttgc agctgaagct ttatcgcata gatgagccgc agcggacaaa ggaaatgctg
     2521 gaaaaaatcg gcgacattac gcgggagcgg gatacgttgg ccgacaagaa caaacgtatc
     2581 cacacaatgc tcaacgaaca gataaaaaag aacgaacaga caggcgagga actcaaggtc
     2641 atacgtaaca actttcagtc gtcggaaaag gaaatcgcca ggctacagct ggagataaaa
     2701 gcctacaagg aagccatggc caatccggca gccgcctctc gcgtggaata cgtcacccgc
     2761 aaacctgtca caacttcgcc tgctgcaaac aaaacgtctt cagccgacga tgaatcgcag
     2821 tcagcctccg acgacgaaga agaatcttcc aacaacaacc ctgtaaaaat cgaaataaac
     2881 cgtatcgccg gcaaaataac cagcatagac gaccagataa tcaaaataaa tgtgggttca
     2941 ctgcatggcc ttcaggccgg catgcgtctg attgtttatc gacgggataa attcgtgggt
     3001 tatctgcgga ttgacatcgt cggagacact actgccgccg gcgccatgtc ccgtcaggtt
     3061 ttcccgcccc agataggtga taacgtcgta gaccgcctat aatgtacaac ataggcaaaa
     3121 aacacggcgt atcaactttt ttggaggtac atcatggcca gaatagctga tatcatcggc
     3181 agagagatac tggattcccg cggcaatccg actgttgagg ttgacgttta tctcgaagac
     3241 ggcacaaggg gtcgggcagc cgttccaagc ggcgcatcca ccggcgaaaa tgaagcctgt
     3301 gaacttcgcg acggcgaccc gaaatactac ggcggcaaag gcgtcctgca cgcggtagcc
     3361 aacgtcaacg gcgaattgcg tgacgtggta gtcggcatgg acgccgaagc gcagaaggct
     3421 gtagacaatg ccatgataca ggctgacggc actaaaaaca aatcgcgact cggtgcaaac
     3481 gcgatactgg gcgtatcgat ggcggtggcg cgggcgcagg ctaattcaat gggtctggaa
     3541 ctttacgaat acctcggcga tgacgattcc gtcatcctgc ccgtgccgat gatgaacata
     3601 cttaacggcg gcaaacatgc cgatggtacg gtcgacttcc aggaattcat gattcagccg
     3661 ctcggcgccg agacgttcag acaagcgctt caaatgggag ctgaagtttt ccattcactc
     3721 aagagtgtgc ttaaaaataa aggctactcc acgggcgtgg gcgacgaagg cggctacgcg
     3781 cccagcctca aaagcaacga agaggcgccg gaattgatac ttaccgccat cgaaaaagcc
     3841 ggttacgccc ccggcgaaga catattcatc gccctcgacc ctgccgccag cgaattggcc
     3901 aacgaagcca gaaaactcgg cagagaaggc tactgtttct tcaaatcgaa cccgtcgcga
     3961 ataatcgata atgaagaaat ggtcgatata tgggttgact ggtgcagccg ttatccgata
     4021 atgagcatag aggacggctg cggcgaagag gactgggacg gatggaaact gctgaccgac
     4081 agactcggcg acaagataca gctcgtcggc gacgaccttt ttgtgacgaa cgtgaatttc
     4141 ctccagaaag gcatagacct gggcgttgcc aacagcatcc tcataaaggt caaccagatt
     4201 ggcactctga cggaaacgct ggcggcgata aatatggccc gccgtgccga ttataccgct
     4261 gtggtttcac atcgttcagg tgaaacggaa gataccacga tagccgacct ggttgttgcg
     4321 gaaaaaaccg gccagataaa aaccggctca gccagccgga ctgaccgcgt gtgcaaatat
     4381 aaccaacttc tacggataga agaaaaactt ggagataaag ctgtttatgg cgcaaggatg
     4441 tggagacaaa attgagatag ttgaggaatc cggcggcgga gcgtttttcc ggcgtcttgg
     4501 ttttttggtg gtgaccggca tggcgctgac attgctggct gcgttggtac ttctgccggc
     4561 gtgggctgaa aaagaaaaaa cccgctatga acttgagcgt gacacgctgc gattgcagga
     4621 ggctaaggaa accgtccagg cgctcagccg catgatgcgc gacgtgcaga acgacgaaat
     4681 tctcacgcaa cgattggccg gcacccgtct tgggctttat acgcccgacg aaattatcgt
     4741 acgcgacccg gcccggccgg tggacaatcc tgaaattcta tcagaaattc attatcctca
     4801 gccgtcgcat aaggactctt tctggctgca aaccgcagcc aaactggaaa aacagggcaa
     4861 acggcgcagc tatatgctgc tttcggcggc tatgctgatt tcggcattca tcctgtttgc
     4921 cccgccgacg cagaagcacg gctggcggcc atggaagttg ccgccaccgc cgataacaaa
     4981 aaaagactga cgactaccga tattacctga gtctgatttt ttgccggctt ggctggcgca
     5041 tattcagagt gtttcggaca ataaagaggc ggctatgaaa aatctgttca tgacaattat
     5101 gtgcattgct gcggctgtcg gcggatgcaa ggaattcgac cctgcaaatt acgacaaaat
     5161 tgaaattcaa tgcgtcagca agaccatcga ctacaggcag aaagtcatag ccgtaaagca
     5221 gggcgacaaa cttcatctca cattctatga catgaactca atcccttcac tgcaattttc
     5281 cgcagattac cagcaggaca gtcgcttcgc caaaaaattc ttcaagctgc ttaatccgga
     5341 aaaaatgata aaaatgggcg actcgacaca aatggaactt accggcggaa gatatgatta
     5401 cagtgtcaaa ttggacaacg atgaaaatgc ttttacgctt tattccccat gcagccttga
     5461 ggtcgtaata gttcctgcaa aagcattggg gcttatggct aataccgata aactatttac
     5521 agaactccaa aacgaagccg gcagaaaaaa tccaaaacat aatgacgacg ctccgacacc
     5581 ctagagcagt tcaatcttga gagttccttt tttgctggtt tgctttgtcc gtggc
//
LOCUS       DAONFI010000137         5605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_16962_length_5605_cov_3.914234, whole genome shotgun sequence.
ACCESSION   DAONFI010000137 DAONFI010000000
VERSION     DAONFI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16962_length_5605_cov_3.914234"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLK08_05620"
     CDS             <1..252
                     /locus_tag="PLK08_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53013.1"
                     /translation="ADRLEPGNAEICFALAEALMDEDQLLQARDYYVRFVKLAGPAWR
                     EYTLSSQSGGDAVMDPRVLRAQRRINEIDYKIRMRETQK"
     gene            complement(261..1031)
                     /locus_tag="PLK08_05625"
     CDS             complement(261..1031)
                     /locus_tag="PLK08_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53014.1"
                     /translation="MLNKNAENGILPGFLDKLRDATPFIELFAYLPDVYFFVKNRQGR
                     FIHVNDIMIEQCGCDEFSQVFGRTDFDFFPHELAQEYVNDDRKVLEKGIHIVNKVELV
                     KNRRGEFNWFLTTKMPLISTTGKIIGLAGYTRDLRRSPHSWRPFETMAPVVEHIRRNL
                     FANIPIGQLAHKACLSVSQFERRFKKIFNISPGRYIMRMKLLEACRELVETQRTISEI
                     ANRTGFYDHSAFTRYFTKLIGMTPKAYRRKFLRINVNG"
     gene            1212..2375
                     /locus_tag="PLK08_05630"
     CDS             1212..2375
                     /locus_tag="PLK08_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS53015.1"
                     /translation="MSRKLRMGMVGGGPGAFIGGVHRKAAAIDGKIELVAGAFDIDPA
                     KSMAMGRELCLDSSRVYSDFREMIAKETALPAERRIDFVAVTTPNNWHFPIAKAFLEA
                     GFNVMCEKPMTMTLAEAEELEQVVKRSGKVFGLMHNYPAYPMVKLARDMVKSGRLGEI
                     RKVVVQYAQGWLARAIEKQGQMQASWRTDPKQSGAGGCVGDIGTHAENLAEYVTGLRI
                     TDICAETTSFIPGRPLDDDVNVLLKFNNGAKGILHASQISIGEENGLRIWVYGSDAAI
                     EWYQEQPNHLTFKPLDKPVEIWSRGNGYVGEISPAAARGTRVPAGHPEGFLEAFANNY
                     SNFADTILSKAAGKEPDALMNDFPQVADGVRGMAFIEAVVKSAKSDRKWTKFE"
     gene            2464..3456
                     /locus_tag="PLK08_05635"
     CDS             2464..3456
                     /locus_tag="PLK08_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPS53016.1"
                     /translation="MSRKVTIFTGQWADLGLEALCRKAAAFGYDGLELACWGDHFEVD
                     KADASYCAEKRELLKKYGLNVFAISAHLVGQAVCDNIDERHQAILPAYIWGDGKPEGV
                     RQRAAEELVRTAKAAKTLGVDVVNGFTGSSIWPLLYSFPPVSPKMIAAGYADFAKRFK
                     PILDEFQKLGVKFALEVHPTEIAFDIVSAQRAIDALDGHPAFGFNYDPSHMGYQGVDY
                     VEFIYRFAPKIFHVHMKDVGWSDKPTQAGVFGGHLDFGDRRRYWDFRSPGRGRIDFEE
                     IIRALNRINYEGPLSVEWEDGGMNREHGAKEACEFVRRVDFAPSNVAFDAAFDK"
     gene            complement(3601..4662)
                     /locus_tag="PLK08_05640"
     CDS             complement(3601..4662)
                     /locus_tag="PLK08_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS53017.1"
                     /translation="MKSLGLGIIGLHHQHPRWYHPLWKNLPEYEPRAIAESDGKFLAS
                     ENEFFRLDAYDDYRKILERDDIDVVIIWLPHSEMPKAVELAAAAGKHVIVEKPCAATL
                     AGAEQIAETAKKYPNIKISSPYCWRTHPVSGQIRKAVVGGLIGEIRAMEARLNAGMAH
                     RYIRDNSAWTLKASEGGGPMWNLGVHWVDYFRWMTGKEVVSICGAVSRISGGPQRDIE
                     DNSQAVLTFEDGATAILDISYSLLDSYPGKRDIYVSMRGNDGNVSWEPAWQGVHDEFL
                     LVSSHEKPESQSRRIHVESKDIPGYCGEMAWTWLKDFAAAVNENRQPMITVDDIIKDV
                     KVVDAFYKSLKTGAIEKVF"
     gene            complement(4680..5342)
                     /locus_tag="PLK08_05645"
     CDS             complement(4680..5342)
                     /locus_tag="PLK08_05645"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPS53018.1"
                     /translation="MNTKTEPKGNKGKRNAQASKQRLLNAAIEVFARKGPGAATVDEI
                     SALAGLNKRLTYHYFGSKELLYKAALYQVYESFFSLEIELSAMLLPADELLETLVRRY
                     YRFLDEHPDFVRMLCFENLNDARAAREMNLQDKKAPIITALRLALQKGQDAKRFRNDI
                     DVADLLISIFSLCFFYFSNRYTMCQLLENNTTSLSNLDKRIKNVVALLLYGITAEKPD
                     SQ"
BASE COUNT         1418 a         1477 c         1391 g         1319 t
ORIGIN      
        1 gccgaccgtc ttgaaccagg caacgctgaa atatgtttcg ctctcgccga ggccctcatg
       61 gatgaagacc agcttctcca ggcgagggat tattatgtcc ggttcgtcaa gctcgccggc
      121 ccggcctggc gcgagtatac gctctcaagt caatccggcg gcgatgcagt catggacccg
      181 cgcgtgcttc gcgcacaacg acgaataaac gaaatagact acaaaatcag aatgcgggaa
      241 acacagaaat aatccggaaa tcagccgttc acatttatcc tcaaaaactt ccggcgatac
      301 gctttgggcg tcatgccgat gagtttcgta aaatatcgcg taaaggcgct atggtcgtaa
      361 aagccggttc tgttggctat ttccgatata gtccgctggg tctcgacaag ttcacggcag
      421 gcttcgagca gtttcattcg catgatgtac cggccgggtg aaatattgaa aatcttcttg
      481 aatcgccgct cgaactggct gacggagaga cacgccttat gcgcaagctg cccgattggt
      541 atgttcgcaa aaagattgcg gcggatatgt tccaccacgg gagccatggt ttcaaacggc
      601 cgccagctat gcggcgaacg acgcaggtcg cgcgtataac cggcaagccc gatgattttg
      661 cccgtcgttg agataagcgg cattttcgtg gtaagaaacc agttgaattc gcctcgccgg
      721 ttttttacaa gttccacctt gtttactatg tgaatgcctt tttcaagcac cttgcggtca
      781 tcgttaacgt attcctgcgc aagctcatgc gggaagaaat cgaaatcggt tcggccgaaa
      841 acctgcgaaa attcgtcgca gccgcattgc tctatcatga tatcgttgac gtgaatgaac
      901 ctgccctgcc gattcttgac aaaaaaatag acgtccggca gataggcgaa caactcgata
      961 aaaggcgttg catcccgcaa tttatcaaga aatcccggca aaataccatt ttcagcgttt
     1021 ttgttcaaca tcggcaggca atagcctcca acaataataa tgcgcatatt atacaagaat
     1081 ttgcgtagtt tttgcaatca tcaatcagcc tttgcggata ttattaataa gaagcaaatt
     1141 tttaccggtt aaattttaat ttaaaattct ggttttaatt tttaaaaacc gttgcacaag
     1201 gagaagaagt catgagcaga aaattacgta tgggtatggt tggcggcggg ccaggcgcgt
     1261 tcatcggcgg cgtccatcgc aaagccgccg caatcgacgg caaaatagaa ctcgtcgccg
     1321 gcgcatttga catcgacccg gccaaaagca tggctatggg cagggaactc tgcctcgact
     1381 cctcgcgagt ttatagcgat ttcagggaaa tgattgccaa agaaaccgca ctgccggctg
     1441 agcgacgcat agatttcgtt gccgtcacca ctccgaacaa ctggcatttc ccgattgcca
     1501 aagccttcct tgaagccggt tttaacgtca tgtgcgaaaa acccatgacc atgaccctcg
     1561 cggaagccga agaactcgag caagtcgtca agcgcagcgg caaggttttc ggactgatgc
     1621 acaactatcc ggcatatccg atggtcaagc tggcgcgcga catggtgaaa tccggccgac
     1681 tcggcgaaat acgcaaggta gtcgtgcaat acgcacaggg ctggctggcc agggctatcg
     1741 aaaaacaggg ccagatgcag gcgtcgtggc gtaccgatcc caagcagagc ggcgccggcg
     1801 gctgcgtggg cgacatcggc acgcatgcgg agaatctcgc agaatacgtc acgggcctgc
     1861 ggataacgga catctgcgcg gagacgacgt cattcatacc cggccggccg ctcgacgacg
     1921 acgtcaacgt actgcttaaa ttcaacaacg gcgccaaggg catactgcac gccagccaga
     1981 tatccatcgg cgaagagaat ggcctgcgga tatgggtcta cggctcagac gcagccattg
     2041 aatggtatca ggaacagccc aaccatctca ctttcaaacc gctggacaag cccgttgaga
     2101 tatggagtcg cggtaacggt tacgtcggcg aaatcagtcc cgcggcagcg cgcggcacga
     2161 gagtaccggc tggccacccg gaaggtttcc tcgaagcgtt tgcgaacaat tattcaaact
     2221 tcgcagacac gattctgtcc aaagccgccg gcaaagaacc ggatgccctc atgaacgact
     2281 ttccacaggt tgccgacggc gtacgcggta tggcgttcat cgaagccgtc gtcaagtcgg
     2341 ctaaaagcga caggaagtgg acaaaattcg aatgaacggc tatattctcc tggtagccat
     2401 gattttttga ggcgattggt tttttttttt aaaactgcga atgccgtatt tgaggtaaga
     2461 gacatgtcaa gaaaagtgac gatatttacg ggccaatggg cggatttggg attggaagcg
     2521 ttgtgccgga aagctgcggc attcggttac gacggccttg aactcgcctg ctggggcgac
     2581 cactttgaag tcgacaaagc cgacgcctcc tactgtgccg aaaaacgcga actgctcaaa
     2641 aaatacgggc tgaacgtttt tgcgatttcc gcacatctcg tcggccaagc cgtctgcgac
     2701 aatatcgacg aacgtcacca agccattctg ccggcttaca tctggggcga cggcaaaccc
     2761 gaaggcgttc gccagcgggc cgccgaagaa ctcgtccgca ccgcaaaagc cgccaaaact
     2821 ctcggcgttg acgtggtcaa cggctttacc ggcagcagca tatggccgct gctctattca
     2881 tttccgccag tgtcgcccaa aatgatagcc gccggctatg ccgatttcgc caaacgtttc
     2941 aaaccgattc tcgatgaatt ccagaaactc ggcgtaaaat tcgcactcga agtgcatccc
     3001 acggaaatag ccttcgacat cgtcagcgcc cagcgtgcga ttgacgcact cgacggccat
     3061 cccgcgttcg gcttcaatta cgacccaagc cacatgggct atcagggcgt cgattacgtg
     3121 gaattcattt acagatttgc cccaaaaatt ttccacgtgc acatgaaaga cgtcggctgg
     3181 agtgacaaac cgacgcaggc cggcgtattc ggcgggcatc tcgatttcgg cgacagacgg
     3241 cgatactggg atttccgttc cccaggcaga ggcagaatag atttcgagga aatcatccgc
     3301 gcactgaatc gcatcaacta cgaagggccg ttgtcggtag aatgggaaga cggcggcatg
     3361 aatcgcgaac acggcgcaaa agaagcctgc gaattcgtca gacgcgtcga tttcgcccca
     3421 tccaacgtcg cgttcgacgc tgccttcgat aaatgacaga aaatgacaga caaaaccaac
     3481 tggttttttt ttaatggaca gaattttcga cgttgaaaaa ggtttccgat gcaaatgccg
     3541 ccagcagtag ccgcaaccag caggatgact gcaggtcagc tttagcatgc tcttagttca
     3601 ttaaaatact ttttcgatag cgccggtttt gagtgactta taaaaggcat ccaccacttt
     3661 cacgtctttg atgatgtcgt caacagtaat catgggctgg cgattctcgt tgacggctgc
     3721 ggcaaaatct ttcagccacg tccaggccat ttcgccgcaa tagcctggta tgtctttgct
     3781 ttcgacgtga attcgccggc tttgactttc gggtttttca tggctgctga ccagcagaaa
     3841 ctcgtcgtgc acgccctgcc aggccggctc ccacgacaca ttgccgtcat tgccacgcat
     3901 tgaaacgtaa atatcgcgtt tcccgggata gctgtccaac agtgaataac ttatgtccag
     3961 aatcgccgtc gcaccgtctt caaaagtcag taccgcctgg gagttgtcct ctatgtcccg
     4021 ctgcgggccg ccggagattc tggatactgc cccgcatatg ctcacaactt cttttcccgt
     4081 catccaacgg aaataatcca cccagtgcac gccgagattc cacatcggcc cgccgccttc
     4141 agaggctttc agagtccagg cggaattgtc gcgtatgtat cggtgagcca tgccggcgtt
     4201 aagtctggct tccatcgccc tgatttcacc gataaggccg ccgacaacgg ctttcctgat
     4261 ttgtccgctc actggatgag tccgccagca atacggcgaa cttattttga tgttcggata
     4321 tttttttgca gtttctgcaa tctgttccgc accagccagc gtggcggcgc atggtttttc
     4381 aactatgacg tgcttgccgg cggctgcggc aagttcaaca gccttcggca tttcactgtg
     4441 cggcaaccat atgattacga cgtctatgtc gtcacgctca agaatttttc gataatcgtc
     4501 atatgcgtcg agccggaaaa attcattttc cgacgcaaga aatttcccgt cgctttctgc
     4561 tatcgccctg ggttcatatt cgggcagatt cttccagaga ggatgatacc agcgaggatg
     4621 ctgatgatgt aaaccgataa ttccaagtcc aagcgatttc atgtttccgt tccgtataat
     4681 tattgtgagt ccggtttttc tgccgtaatc ccatataaca gcagagccac aacgtttttt
     4741 atccgcttat ccagattcga cagcgatgtc gtgttgttct ccaaaagctg gcacatggtg
     4801 tacctgtttg aaaaatagaa aaaacacaac gaaaatatac ttatcagcag gtcggccacg
     4861 tcgatatcat tgcgaaaccg cttcgcatcc tggcccttct gcaacgccag ccgcaaagca
     4921 gtaattatcg gagccttctt gtcctgaaga ttcatctccc tggcagctcg ggcgtcattt
     4981 agattttcaa aacacagcat ccgcacaaaa tcaggatgct catccagaaa cctgtaatac
     5041 ctgcgaacca gcgtctccaa aagctcatca gccggcagca gcatggcgct aagttctatt
     5101 tcaagcgaaa agaaactttc ataaacctga tacagcgcgg ctttgtacaa cagttccttg
     5161 cttccgaaat agtgatatgt cagccgcttg ttaaggccgg caagcgcaga tatctcgtca
     5221 acagtcgcag cacccgggcc ttttctggca aaaacctcta tggcggcatt cagcagacgc
     5281 tgcttgctgg cctgggcgtt ccgtttacct ttattaccct tcggttcagt tttagtgttc
     5341 atgcctgttt tatagcattt atgcaaacaa atgtcatcag aattatagta accgattagt
     5401 tacaattaat caacaaaaat ttaattatgc tcttgaaaaa gagaaaactt gggatatcct
     5461 ttaattatgc atgaacagga gtatttccag tggcaaatat aaaagttgtc gttaccgatt
     5521 acattgaaac cgacatggat tgggaagcgg cggaaatggc caatcgcggc gtcagatttg
     5581 aggcgtatca gctcaagcag gccgg
//
LOCUS       DAONFI010000138         5580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17046_length_5580_cov_3.626063, whole genome shotgun sequence.
ACCESSION   DAONFI010000138 DAONFI010000000
VERSION     DAONFI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17046_length_5580_cov_3.626063"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLK08_05650"
     CDS             complement(<1..827)
                     /locus_tag="PLK08_05650"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPS53019.1"
                     /translation="MRLLRGGWRYSGIRDILRILVASWWFVLISSIFLVLFVYMPDSM
                     GKMKPRYIGDYFAFFPKSVLVLDFMVTVFLVSVARLALRIYYEELRPISPEGLKRVLL
                     VGAGSAGETILRELSRTRYEEYRVVGIVDDDPHKQGLLIHDIPILGKTDDIPRICKEN
                     DIREIIIALPSASQKQMKRVINLCSGTKLKFQALPGLSDIISGKVMVSQIRPVDINDL
                     LGRDIVQLDTKAISSFINGRIVLVTGAGGSIGSEMCRQICSYSPQRLIIVEQAETAL"
     gene            1277..1720
                     /locus_tag="PLK08_05655"
     CDS             1277..1720
                     /locus_tag="PLK08_05655"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HPS53020.1"
                     /translation="MKHWRWVILAAGTECFCMIGCSEELESHANQFSLNPVIINESQA
                     GKSVSVHPGQSVEIKLAANATTGYQWMETSFNTFSSSPAVLERIGSEYHPSQAGNVAV
                     GTGGTYLSKFKAVEKGRVKITLKYIRPWESQTPAAKTLSFEIIVD"
     gene            1747..3291
                     /locus_tag="PLK08_05660"
     CDS             1747..3291
                     /locus_tag="PLK08_05660"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPS53021.1"
                     /translation="MKTPPKLLSESSSGDKDNKATGIELLLDEEVLEILVGLAQVIYR
                     YDYQAGKLAYITANCKNIMGHEQAQLVGKEWQDIARNIHPSDLTFVSRRRNEVLEHTR
                     HDSATVGNIEYRWKKDGKYIWLRSRFHFTLNSEGKTQFETGLLIDVTHSRNLYDSMDT
                     EQQWNLSLIDAIPDMIFRFNRAGVIVGYKPAAGLQPVVPPEVFMGRPYNEVLPPDISD
                     LFDKTCEKVRHDGTVETIEYELKIEGEIHTYEARAIHSGVGDVLLVVRDITENKRSKA
                     ALLRVQNDLENRVDRRTHELRELHKKLLNASETERRRLARELHDSVAQDLIVLQLLLK
                     QYSPDAKNFEQQQTTVQNITNKLITDIRRICQGLYPPVLESLGLYQALKSLEEYHQMT
                     GMNVEIRWEAASRPTRLKQDLEIGLFRIAQEALSNAFKHGKAQKAIVELYSSADGAKL
                     TMTITDNGCGEVDPEKVKSGFGLQTMQDRANAMGGTFAFLPVPHGTCVCVEVPLATSE
                     EDDSFM"
     gene            3501..5126
                     /locus_tag="PLK08_05665"
     CDS             3501..5126
                     /locus_tag="PLK08_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPS53022.1"
                     /translation="MAFELKEFQDSTLDAEFVAYMIDGQAPENELFYNRLWNYFRNPI
                     IPLCGVSAATMNENSRPYFLAQEMGLPARITGIIRNASGDAPTDLRRKEVVIENDIAW
                     RISCMVDFLFGRPINIRSSAEEPTQAAEIEKIISTLFSANGGAAFFQELALFGAVYGF
                     VDIALRIPADWPAEPISALRTFNPKTAGKNAAELHESPDAHSAAGRPGISLQNHSAQD
                     TANVEDRSEQLNAALDWVRKITLETVEASRVLPVLAEDDYRTIRYWVQRYFKAIPRLA
                     TSRRGFMGLLGGRSKVQRPEMAEVVEIIGDNWWQRYEDRMLVAHGPNLLSRVPVVHLQ
                     NIASPGTYGGLSDVEPMIPLQDELNTRLSDRANRVTYQSFKMYLGKGIDDFLERPVGP
                     GQMWATQNLNASIEEFGNDEGSPSEDIHIDQVRQAMDKVSGVAPLAAGLLSGKVGNLT
                     SATALKVVLSGLISRTNRKRLTYGKAITDIIAMTLEWLDKTGVYRTTPQQRKVELHWP
                     SPLPNDETQLLQDAKLKLELGVPAERVLAELGY"
     gene            5153..>5580
                     /locus_tag="PLK08_05670"
     CDS             5153..>5580
                     /locus_tag="PLK08_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53023.1"
                     /translation="MTQEIEFENDSSLGSENGAKVVPVAEAIKYRRRAQQAETRIAEF
                     EQKLDALQSQLATQNDELQQARENERQTSLELSEMNNRIKVDNALSQAGVIDPEAARL
                     LLKERLDIFGDIDDRQLKTAIGELLTNKPYLKSSHTTGGL"
BASE COUNT         1650 a         1334 c         1317 g         1279 t
ORIGIN      
        1 aaaagagccg tctccgcctg ttccacaata ataagtcttt gcggcgaata gctgcatatc
       61 tggcggcaca tttcagaacc tatactgccg cccgcaccgg ttacaagaac tatacggcca
      121 ttgatgaaag aactgatggc tttcgtgtcc agttgcacga tatcgcgacc cagaaggtcg
      181 ttaatatcga caggcctgat ttgcgaaacc attaccttgc cgctgataat gtcgctcaag
      241 ccgggcaacg cctgaaactt caatttcgta ccactacaga gattgatgac ccgtttcatc
      301 tgtttctggg atgcggacgg cagagcaatg ataatttcac ggatgtcgtt ttccttgcag
      361 attcgcggaa tatcatcggt tttgccgagt atgggtatgt catgtattaa caggccctgt
      421 ttatgcggat cgtcatcaac tatgccgacg acccgatatt cttcatacct tgtgcgactc
      481 agctcccgaa ggatggtttc gccagcactg ccggcgccga caagaagaac ccgtttaagc
      541 ccttcaggcg aaatcggccg aagttcttca taataaatcc gcaacgccag cctggcaaca
      601 cttacaagaa aaaccgtaac cataaaatcc agaacaagaa cacttttggg aaagaaagca
      661 aaataatcgc cgatatatcg gggcttcatt ttccccatgg aatccggcat atacacaaat
      721 aatacgagaa atatcgaaga aatcaggaca aaccaccagg acgccaccag gattctcaat
      781 atatcccgaa tgccagaata tcgccagccg ccacgaagca accgcatttt atgaaatata
      841 aacgtcttta atataataaa atatggcaaa attatgaaaa actggccttg aagccaattc
      901 ctattcttcg cctgcgcacc gaggtcaaaa cgtatcagaa atgaaaaaaa caaagatatg
      961 gcaaaaataa caacatgaat accaacagaa cagaatattc tatggttaag ataaaaatcc
     1021 aaaatccagg atggcttggg attgtcagtt ttatttatat tctgcgtttc gtccataatg
     1081 tctttgccgt acaaatagaa tcgctttaat tatagttaca cacatattaa actcaagtaa
     1141 cagtttgtct gcaagagtac ttgagtatat tatcggttta ttaacataaa aacttaaatt
     1201 tattaatttt aagagaattc cttgagttga aaattaaatg catataatga aatggcacga
     1261 caggaaagga gaagagatga agcattggcg ttgggtgata ctggccgcag gtacggagtg
     1321 tttctgcatg attgggtgtt ccgaagaatt agagtcccat gcaaatcaat tttccctcaa
     1381 ccctgtaatt ataaatgaaa gccaggctgg caagtctgta tccgtccatc cagggcaatc
     1441 tgtcgaaata aaactcgccg ccaacgctac tacaggttat caatggatgg agacttcgtt
     1501 taatactttt tcgagttcgc cggctgtttt ggaaagaatc ggcagtgaat atcatcccag
     1561 ccaagccggc aatgtcgccg tcggtacagg tggaacatat ttgtcaaaat tcaaggctgt
     1621 cgaaaaaggc agagtcaaaa ttacgctaaa atatatcagg ccgtgggaat cacagacgcc
     1681 tgccgcaaaa acgctgtcat ttgaaataat tgtcgattga aaatcatgaa aatcggccaa
     1741 gacacaatga aaacgcctcc aaaactgtta tcagaatctt ccagtggcga caaagacaat
     1801 aaggccacag gaatagaact gttgctggat gaagaagtgc tggaaattct tgtcggactg
     1861 gcacaggtca tatacagata tgactatcag gcaggaaaac tggcatatat aactgcgaac
     1921 tgtaaaaata tcatgggcca cgaacaggct caactcgtcg gcaaagagtg gcaggacatt
     1981 gccagaaata tacatccatc tgacctgacg tttgtcagcc gtcgcagaaa tgaggtattg
     2041 gaacatacgc gacacgattc tgcgacggta ggtaatattg aataccgctg gaaaaaagac
     2101 ggcaaatata tctggttgcg ttcgcgattc cattttaccc tcaatagcga gggcaaaacc
     2161 caattcgaaa ccggcctgct aattgacgtg actcattcac gaaatcttta cgacagcatg
     2221 gataccgaac aacaatggaa tctttcgctc atagacgcca ttcccgacat gatttttcgt
     2281 tttaacaggg ccggggttat cgtcggttac aaaccagctg caggtttaca gcctgtcgtt
     2341 ccgccagaag ttttcatggg tcggccttat aatgaagttt tgccgccgga tatcagtgac
     2401 ctgtttgaca aaacgtgtga aaaagttaga cacgacggca cggtggaaac gatagaatac
     2461 gaattgaaaa tcgagggaga aatccatacg tacgaagcac gtgccattca tagcggcgtt
     2521 ggcgatgttc tgcttgtcgt gcgcgacatt accgaaaaca aaaggagcaa agcagcacta
     2581 ttgcgggtgc agaatgattt ggaaaatcgc gtcgatcgcc gcacgcacga attgcgtgag
     2641 ctgcacaaaa aactgctcaa cgccagcgaa accgaacgcc gccgtcttgc ccgcgaattg
     2701 catgactcgg tcgcacagga tttgatagtc ctgcagttgc tgcttaaaca gtactcaccc
     2761 gacgccaaaa atttcgaaca gcagcagact acggtacaaa acatcaccaa caagctcata
     2821 acagacatcc ggcgaatatg tcagggttta tatccgcccg tgcttgaatc gctaggcctt
     2881 tatcaggctc tcaagtcact ggaagaatat catcagatga ccggcatgaa tgttgaaata
     2941 cgctgggagg cggcatcacg gccgacgagg cttaaacagg atttggaaat aggccttttc
     3001 cgcatagcac aggaagctct cagcaatgca ttcaaacacg gcaaagccca aaaggctatc
     3061 gttgaattat attcatcagc tgacggggca aaattgacaa tgaccataac agataacggc
     3121 tgcggggaag tagaccctga aaaggtaaaa agcggtttcg gcttgcaaac catgcaagac
     3181 agggcaaacg caatgggcgg aactttcgct tttctgcccg tgccgcatgg aacgtgtgtc
     3241 tgtgttgagg taccactggc aacatctgaa gaagatgaca gcttcatgtg aaatatccag
     3301 accctgcgct agccgtttta aaccatagat gacactttag cattttttat cataaaattt
     3361 tttctatccg cttttacggg gttttattca ctttattcaa ttctgaacgt aatgcttttt
     3421 gctctcgcga acgtctgcgc tgccggaaag gatgaaagac agcaaaggcc tccggcaggc
     3481 cgatttttgg agcgagagtt atggcatttg aattgaaaga atttcaggat tcaacgcttg
     3541 atgcagaatt cgtggcatac atgattgacg gtcaggcacc tgaaaacgaa ttattttata
     3601 accgtctctg gaattatttc cgcaacccca tcattccgct ttgcggcgtt tcagccgcga
     3661 cgatgaatga aaactccagg ccatattttc tggcgcagga gatgggtctg ccggcaagaa
     3721 ttacagggat tatccgaaac gcctccggcg atgctcctac tgatttgaga cgcaaggaag
     3781 tcgtcattga aaacgacatt gcctggcgaa tttcctgcat ggtcgatttt ctgtttggcc
     3841 ggccgataaa catccgcagt tctgccgaag aaccaacgca ggctgctgaa atagaaaaaa
     3901 tcatctcgac actgttttca gccaatggcg gcgctgcatt ttttcaggaa cttgcattat
     3961 ttggcgccgt ttacggcttc gttgacatag cccttcgaat cccggccgac tggcctgccg
     4021 aaccaatctc agcccttcgt actttcaacc cgaaaacagc gggcaaaaac gcggcggaat
     4081 tacatgagtc tcccgatgct cattccgctg cgggtcggcc gggcatatct ttacaaaatc
     4141 actccgcaca ggacacggct aacgtggaag atcggtctga acagctgaac gcagcccttg
     4201 attgggtcag aaaaatcacg ttggagaccg tcgaggcttc cagggtactg ccggtcctgg
     4261 cggaggatga ttatcgcacc atacgttatt gggtgcagag atattttaag gctattccgc
     4321 gtctggcgac gtcgcgtcgc ggcttcatgg gcctgctcgg cggccgcagc aaagtccagc
     4381 ggccggaaat ggcggaagtc gtcgaaatca tcggcgataa ctggtggcag agatatgaag
     4441 accgtatgct cgtggcacat gggccaaatc tgctcagccg cgtacctgtc gtgcatctcc
     4501 agaatatagc gtcaccaggc acatacggtg gccttagcga cgtcgaacct atgataccac
     4561 tgcaggacga actcaacacg cgcctttccg acagggctaa ccgcgtaacg tatcaatcat
     4621 tcaaaatgta cctcggcaaa ggcatagatg attttcttga acggccggta ggccccggtc
     4681 agatgtgggc gacgcagaat ctcaatgcga gcatcgagga attcggaaac gacgaaggct
     4741 caccgagcga agacattcac atcgaccagg ttcgtcaggc gatggataaa gtttcaggcg
     4801 tcgcaccact cgccgccggc ctgctgtcag gcaaagttgg aaatctgacg agtgcaaccg
     4861 cgctcaaggt tgtgctcagc ggccttataa gtcgtaccaa tcgcaaacga ctgacctacg
     4921 gcaaagctat tactgacata atcgcaatga cattggagtg gctggataaa accggcgtat
     4981 atcgtaccac cccgcaacag agaaaagtgg aactgcattg gccaagcccg ctgccgaacg
     5041 atgaaactca actgctccag gacgcaaaac tcaaactgga actcggcgtt ccagcggaac
     5101 gagtacttgc ggaactggga tattgaaaat cataatttca caggaataaa acatgacaca
     5161 ggaaatagaa ttcgaaaatg attcttcgct tggcagcgaa aacggcgcaa aagttgttcc
     5221 ggtggcagaa gccatcaagt atcgccgtcg cgcccaacag gcagaaaccc gcatcgcgga
     5281 gtttgagcag aagctcgacg cgcttcagtc gcagctggct acgcaaaatg atgaactcca
     5341 gcaagctagg gaaaatgaac ggcagacctc gttagagttg tcagaaatga acaacaggat
     5401 aaaagtcgac aacgcacttt cgcaagccgg cgtaattgac cctgaagcag ccagactgct
     5461 ccttaaagaa cggctggata tttttggcga tatcgacgac agacaactca aaaccgccat
     5521 aggtgaactg ctgacaaaca agccctatct caagtcatca cacacgaccg gcggattaat
//
LOCUS       DAONFI010000139         5574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17079_length_5574_cov_3.892191, whole genome shotgun sequence.
ACCESSION   DAONFI010000139 DAONFI010000000
VERSION     DAONFI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17079_length_5574_cov_3.892191"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLK08_05675"
     CDS             complement(<1..658)
                     /locus_tag="PLK08_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPS53024.1"
                     /translation="MESHLGLLFVLGIGVLGGTIGALLFQKLKVPQVVGYIGIGLLIG
                     DTGFGIVPSDQIAQFKQLNLFALGIIGFLVGGELSGDIFRKYGKQFMGILMGEGLAAF
                     ILVGIASGLVIYFVVHNTAAAVAAGIVFGAIASATDPASTIDVLWEYRSRGILTTAII
                     AIVALDDALAMTLYGLGTSAAGMLMGENASFAAAFMKLSVELFGSILLGGVAGLLLNW
                     I"
     gene            complement(660..1340)
                     /locus_tag="PLK08_05680"
     CDS             complement(660..1340)
                     /locus_tag="PLK08_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPS53025.1"
                     /translation="MQAQDIWTVEDVANYLRVSERTVYEWAQKGVIPCGKLGTVWRFK
                     REDVMRWVDENLTAVESYRQQPAQEQMLTDVLSQERIIIAENADKEQALCMLCETFSD
                     CDAVEDVEQLEQRIFYRESLLSTGIGLGCGIPHCRLKNIRKPVMAVGIFPDGIKDYTS
                     LDNEPVKLVFLIAAGDGQHEEHIKLLSKVSELIKTAEFRNRLVKAADVNEAWQILTQP
                     YPTITQEK"
     gene            1865..3037
                     /gene="dxr"
                     /locus_tag="PLK08_05685"
     CDS             1865..3037
                     /gene="dxr"
                     /locus_tag="PLK08_05685"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPS53026.1"
                     /translation="MGNSIVILGSTGSIGRQTLDVLAGMPGLRACGLGAGGGNAELLV
                     EQAISARPDFVAVANRNVAEKMAQSMPADTTLLSGDDALCEMIRLSKPDVIVSAVVGA
                     AGLLPTLEGIRQGSTIALANKETLVCAGSVVMPAARNAGVSVLPVDSEHSGIFQCMLA
                     GKSSEISKVIITSSGGSLRDWDDASAASASVADALRHPTWQMGRKITIDSATLINKTL
                     EVIEAHWLFGLPADKLEVVIHPQSIIHAMVEFCDGSTIAQLAKPDMRLPIAYALNYPD
                     RPQRKVAPLNLEMLRNLEFRPVKGRFRKAVELARRVIEDGACTGSVLNAANETAVEAF
                     LAGRIKFGQIVPSIEAALDRWEKEGDTKNPQPSLDDILAADAWARKYAEKNLTTIV"
     gene            3045..5096
                     /gene="rseP"
                     /locus_tag="PLK08_05690"
     CDS             3045..5096
                     /gene="rseP"
                     /locus_tag="PLK08_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPS53027.1"
                     /translation="MQFLNSLIFDWIWPLIEFLIGLNIVVFFHELGHYLTARKVGIKV
                     ERFALGMGPRLFGIIRGGTDYCVCALPLGGYVKMLGQEDAGEVSESAAADPRSFLQKT
                     VGQRFLVVSAGVFMNIVLACLIYVIICMVGIDVYKPIVGGVQPTYPAATAAITWQGGQ
                     GPAGCDNMGLKAGDVITAVNGSDVEQFKNVQFASLLARRDEKVQLDFDRSADGRTYRG
                     SVELGVRPGGIKGLLSFGIDAPASNRVWGAAVDSQLKINDEIIAINGKKVANEWEMEH
                     AGDELTNGPVRLTVLRDGEEKQLTVTPIPYNRSDMIFLKNGRVIYGLPEKVDGDRTVI
                     ILDNGRQIDVVTKNIASGNGSGLDVLGLSPRLMTYDTKKWKGIFRHTPAWNAGIKNGD
                     ILLSYDETVNPTQSEILQISANLLKEKRSGELVCLRDGEKKTFEIEPYKSEGVALIGV
                     SFTGDYEHPVAGFVRHGSPAAKAGIKSGSRIIAVGSRPVKNWMDIVWAVKALPKETRV
                     LPITTDAGEFSLNIPESFNASDIRLSVLGMVALRPDTFKLHRKNPLAAMAWGAKEAWE
                     TILLNYASLRAIATGTVPAREASGPLGLGKMAIDQAKHGIVPLMYLMAFLSAALAVFN
                     FLPLPVLDGGIATLLIIEKLRGKPLPLKVINMIQLAGLVFILGLFILVTFSDFLKLFD
                     I"
     gene            5192..5350
                     /locus_tag="PLK08_05695"
     CDS             5192..5350
                     /locus_tag="PLK08_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPS53028.1"
                     /translation="MKYVCDVCGYIYDPAEGDPDNGIPAGTRFEDLPDDWVCPECGAG
                     KEDFSPMK"
     gene            5353..>5574
                     /locus_tag="PLK08_05700"
     CDS             5353..>5574
                     /locus_tag="PLK08_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53029.1"
                     /translation="MGKTPVRRRSELQCRTGCDFDGAWAMAVRLWGLFAGWRCNNTYL
                     RISGGRFGIFPACDVVLRFLFLAAVCMPAS"
BASE COUNT         1328 a         1373 c         1559 g         1314 t
ORIGIN      
        1 gtatccagtt gagcagcagc ccggctacgc cgcccagcag aattgaaccg aaaagttcca
       61 cgctcagctt catgaatgcc gctgcaaacg aggcgttttc tcccattaac atgccggccg
      121 ccgaagtgcc cagcccgtaa agcgtcatag ccaacgcatc gtcgagagct actattgcga
      181 ttattgccgt cgtgagaatt ccgcggctgc gatattccca aagaacgtct attgtcgagg
      241 ctgggtctgt ggctgaagct atcgcaccga acactatgcc ggctgccacg gctgcggcag
      301 tgttatgcac gacgaaatat atcaccaaac cgctggcgat gcccacgagt atgaaagctg
      361 ccaaaccttc acccatcaat atgcccatga actgcttgcc gtatttgcgg aaaatgtcgc
      421 cgctgagttc gccgccaacc agaaaaccta ttatccccag tgcgaacagg ttcaactgct
      481 tgaactgcgc aatctggtcc gacggcacta tgccgaagcc cgtgtcgcct ataagcaggc
      541 ctatgccgat atatcccaca acctgcggca ctttgagctt ctggaacagc agtgcgccta
      601 tcgtgccgcc aagtacgcct atgccgagaa cgaacagtaa accaagatga gattccatcc
      661 tacttctcct gagtaatcgt cggataaggc tgggtcaata tctgccaggc ctcgtttacg
      721 tcggcggctt tgactagacg gttgcggaat tcggcggttt tgatgagttc gctgactttt
      781 gagagcagct ttatatgctc ctcatgttgt ccgtcgccgg ctgcgataag aaataccagc
      841 ttgacgggtt cattgtcgag tgaagtgtaa tcttttattc cgtcggggaa aatgccgaca
      901 gccattaccg gcttgcgtat gtttttcagc cggcagtgcg gaatgccgca acccaaccct
      961 atgccagtgc tcaacaggga ctcgcgataa aatatacgct gttcaagctg ctctacgtct
     1021 tcaaccgcgt cgcagtcgga aaatgtttcg cagagcatgc acaaggcctg ttccttgtcg
     1081 gcattttcgg cgattatgat tcgctcctgc gaaagtacgt cggtgagcat ctgttcctgt
     1141 gccggctgtt gccggtagga ttcgacggcg gtaagatttt cgtcgaccca acgcattacg
     1201 tcttcacgct tgaatcgcca gacagtccca agtttgccgc agggtataac gcctttctgc
     1261 gcccactcgt aaacagtccg ctcagatacg cgaagatagt tcgccacgtc ttcaacagtc
     1321 cagatatcct gcgcttgcat gatgaccgat acctcctgta actcttgtgc tgcctgtatt
     1381 ttcgccgata atccacttcc gaccgacctc agccgtatag tgggataacg cgatgccatt
     1441 ttgccatcaa ctgccaaata catctctcaa acgaaccttc ctgtaccata tgcgttatac
     1501 agtaatatat gcaatatatt cgattatata cgtaattaaa gtaaaaaaat cggtaatttt
     1561 ctaatttatt cgctttattt ggagctgctt caagatttat ttttgacact gcaggattca
     1621 aaaggctgac tgcaacggaa agaatattga gaatcacact gaaatattgt agggcgatga
     1681 aaatgtcggc tgatgaattt ttgaaaagtg ttgaaagaaa aatatttttg tggagaatat
     1741 gccaatctct gtgtaatgca tgcaggcttt aaggctcggc cagttgtgac gagcagtttt
     1801 ctccgaatgc aagcctgtgc atctgcataa gataagtaac caggattatt ccaggagcag
     1861 ttttttgggt aattcaatag tcatattagg ttcaaccggc agtatcgggc ggcagactct
     1921 ggatgtgctt gccggcatgc cgggtttacg tgcatgcgga ttgggggcag gcggaggcaa
     1981 tgcagagctg ctcgttgagc aggctatcag cgccaggcct gatttcgtgg ctgtggccaa
     2041 tcgaaacgtc gctgaaaaaa tggctcagtc catgcctgcc gacacaacac ttctgtccgg
     2101 cgacgatgcc ctttgtgaaa tgatacgcct ttcaaaacct gacgtcatag taagtgctgt
     2161 agtcggagca gccggccttc tgccgacgct ggagggcata cggcagggca gcacgattgc
     2221 gttggcgaac aaggaaacgc tggtttgcgc cggcagcgtg gttatgccgg ctgccaggaa
     2281 cgcaggcgta tccgtattgc ccgtcgacag tgaacattcg ggcattttcc agtgcatgtt
     2341 agccggcaaa agcagcgaga taagtaaagt catcatcacg tcgtccggcg gctcgttgcg
     2401 ggactgggac gatgcgtcag ccgccagtgc aagcgtggct gacgcgttgc gacatcctac
     2461 atggcagatg ggccgcaaaa taaccatcga ctctgccacg ctgataaaca agacgctgga
     2521 ggtgatagag gcgcattggc tgtttggcct gccggctgac aagctggaag ttgtcataca
     2581 tccgcagagc atcatacacg ccatggttga attctgcgac ggctcgacga ttgcccagct
     2641 tgccaagcct gacatgcgtc tgccgattgc ctatgccctg aattatcccg accggccgca
     2701 gcgtaaagtg gcgcctctta atcttgagat gctcaggaat ctggaattcc ggccggtgaa
     2761 aggccgtttc cgcaaagccg tcgaactcgc ccgacgagtc atcgaagatg gcgcatgtac
     2821 cggctcggtt ctgaatgcag ccaatgaaac tgccgtcgaa gcatttttag ccggcaggat
     2881 taaattcggc cagatagttc cgtctataga agcggctctt gaccggtggg aaaaagaggg
     2941 cgatacgaaa aatccccagc cgtcgctgga tgatattctg gcggctgacg catgggccag
     3001 aaaatatgca gaaaaaaatc tgaccaccat cgtatgaggt agttatgcaa tttttgaaca
     3061 gtttgatatt cgactggatt tggccgctga tagaatttct aatcgggctt aacatcgtgg
     3121 tatttttcca cgagctgggc cattacctta cagcccgcaa ggtcggcatc aaggtcgaac
     3181 ggtttgcgct gggtatgggg ccgcggctgt tcggcataat acggggcggt acggattatt
     3241 gcgtctgcgc gctgccgctt ggcggatacg tcaaaatgct cggccaggaa gacgcgggcg
     3301 aagtttccga atctgccgcc gccgacccac gctcatttct gcaaaaaacc gtcggccaac
     3361 gttttctggt ggtatcagcc ggcgttttca tgaatatagt gctcgcctgc ctgatttacg
     3421 tgataatatg catggtgggc atagacgtgt acaaaccgat agtcggcgga gttcagccga
     3481 cgtatcctgc ggctacggca gcgataacat ggcagggcgg ccaggggccg gcgggatgcg
     3541 ataacatggg cctgaaagcc ggcgacgtca taaccgccgt caatggcagt gacgtcgaac
     3601 agttcaaaaa cgtccaattc gcttcgctgc tggccagacg cgatgagaaa gttcaactgg
     3661 attttgaccg cagtgcggac ggcagaacgt accggggcag cgtcgaactg ggcgtaaggc
     3721 ccggcggaat caagggattg ttgagtttcg gcatagatgc gccggcgtcc aacagggttt
     3781 ggggggcggc ggtggattct cagctgaaga taaatgacga aataatcgct attaacggca
     3841 aaaaagtcgc aaacgaatgg gaaatggagc atgcaggcga cgaactcacc aacgggccgg
     3901 tcaggctgac ggttctgcgc gacggcgaag aaaagcagct taccgtaacg cccataccat
     3961 ataatcgcag tgacatgata tttctcaaga acggcagggt aatatatggc ctgccggaaa
     4021 aagtcgatgg cgacaggact gttataatcc tggacaatgg ccgccagatt gacgtagtga
     4081 caaaaaacat cgcatcaggt aacggcagcg gcttggatgt gcttgggctg agtccgcggc
     4141 tgatgacgta tgacaccaag aaatggaagg gcattttccg ccatacgcca gcctggaacg
     4201 ccggcataaa aaacggcgat attttactga gttatgatga aaccgtaaat ccgacgcaga
     4261 gcgagatatt gcagatatcg gcgaaccttc tcaaggagaa gcgttctggc gagctggtgt
     4321 gcctgcgtga cggcgagaag aaaacctttg aaatcgagcc gtacaagagc gaaggggtgg
     4381 cacttatagg cgtaagtttt acgggcgatt atgagcatcc ggtggcgggt tttgtgcggc
     4441 atgggtcgcc ggcggcgaag gctggcataa aatccggcag caggataatt gccgtcggca
     4501 gccggccggt caaaaactgg atggatatcg tctgggcggt caaggctttg ccgaaggaaa
     4561 ctcgtgtgct tccgattacc actgatgccg gcgaattcag cctgaatatt cccgaatcgt
     4621 tcaatgcgtc ggatattcgc cttagtgtgc ttggaatggt tgcattacgg ccggatacgt
     4681 tcaaactgca tcgtaagaat ccgttggcag ctatggcatg gggcgcgaag gaagcctggg
     4741 agacgattct tttgaactac gcctccctgc gggcgattgc caccggtact gtgccggctc
     4801 gcgaagccag cggccctctc ggcttgggca agatggcgat agaccaggca aaacacggca
     4861 ttgtgccgct gatgtatctg atggcgtttc tgtcggctgc gctggcggta ttcaactttc
     4921 tgccgctacc ggttctggac ggcggcatag ccacgctgct tatcatagaa aaattgcgag
     4981 gcaaaccgct gccgttgaag gttatcaata tgatacagct ggctggcctg gtattcattc
     5041 tcggcctgtt tatactcgtg acttttagcg atttcctgaa attgttcgat atttagagtg
     5101 atttgccgaa gcacattttt gattttcggc gtttaaacgt gtacaatgac gcagtgtcga
     5161 gtttttgtgg caaatgcgga ggtaaagtgc gatgaaatac gtttgtgacg tttgcggtta
     5221 tatttacgac ccggcggaag gcgatccgga taacggcata ccggccggca cgaggttcga
     5281 agacctgccg gatgattggg tgtgtccgga atgcggagcc ggcaaagaag atttttcgcc
     5341 catgaaatga aaatgggaaa aacacccgtc cgacgtcgaa gtgaacttca atgtcggacg
     5401 gggtgtgatt ttgacggagc gtgggcaatg gctgttcgat tgtggggctt gtttgccggc
     5461 tggcggtgta ataatacgta tctccggatt tccggcggac gattcggaat cttccctgcg
     5521 tgcgacgtgg tactcagatt tctattcctc gcagcggttt gtatgccggc ttcc
//
LOCUS       DAONFI010000140         5503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17387_length_5503_cov_4.357012, whole genome shotgun sequence.
ACCESSION   DAONFI010000140 DAONFI010000000
VERSION     DAONFI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17387_length_5503_cov_4.357012"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLK08_05705"
     CDS             <1..306
                     /locus_tag="PLK08_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53030.1"
                     /translation="QQNDHLVMFRKLPDNITISDRFRTNFQVSETIRILGMPPKPDPN
                     TGKVPGLGLTYSQIVSFLYRMTQQNNIKAKFVLQPLPEIRRIYEIIPSVGRHDVENP"
     gene            363..3983
                     /gene="smc"
                     /locus_tag="PLK08_05710"
     CDS             363..3983
                     /gene="smc"
                     /locus_tag="PLK08_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPS53031.1"
                     /translation="MKLKKLILAGFKSFADKTEFDFDDGISCVVGPNGCGKSNVVDAV
                     KWVLGEQSAKSLRGSEMMDVIFNGSSARKPSGRASVTLVFDNSDSVLKPVLNGATQEC
                     PEVSVTRRLFRSGQSEYLINNQPSRLRDIREMFLDTGLGANAYSIIEQGKVSAFLQAG
                     RDERRAFFDEAAGISRYKARKKEAIRKLERVEQNLLRVMDIMAEVEKRLRSIKVQAGK
                     ARNYQTYADRLNELRALFFLSRFHTMKNQRKMQADELQSGSLSLETIEEQIAQLENAQ
                     RAVEVEAAQIQETTREIQARISNINAQMTTLHERVEMQNRRMEELVESLEVNNLRTAE
                     LAQRLEESAGDIELREVELQQVAEAAEELRQQTQAIIDAYSAAEMHIADIRREIEDRR
                     SGMNELERQEGQLKNEAHAASLKCESLTGEKQRVADRLAELQNQIESIAGGKTELETR
                     MTQAIAAIESGEEQLGQLTAVSEELTASELSLGHELADAREQRSRQQGRMHTLEEMQK
                     RMVGVTEATRLVIQAKQDGKFPAIQGMLGDFIETDTEHAPLVEAALAGADQRLVAATF
                     AEVQQISAQIEEMFSEGGSVEIICADLAGRRPVDDATVNCPQAVGRVLDFIKYEPSLE
                     PLMWNMLGRTLMVRTLADAACALENTPAGYRFVTLAGEVLEPDGMVRVGSASHAAGMI
                     ARKSEMQEITQNLSVLDGQVEALQTRCEETRRQREQLEEQLQSVRSTLYEARFEKSES
                     SRQIEQLTAKINALTAEQPGLAEKMEHLTSQISQASRQQATAMQQTEQVAADRVRLAE
                     SITAMETRLAESQQSIEQLNEKRTELKVAVAENEQKRLSLRDSLESIRRQREQMQAEL
                     EKARNNLELDRQRREQAEQEIASAQEEGRQLVGRLQEMQQEYNDSEESRRSLSERLVE
                     IRQQLAENRQSEHDANRLVGNMRVKLSELDAHITDIIERAAHEMEMNLNELYGSYSHD
                     AERDWDAVEAEINDLRTKISRLGNVNMDAISEQDELEKREEFLRTQLDDINNSRKQLE
                     DLINRLNVESRERFVETFNAVRENFQSLFRKLFGGGKADIMLSEEEDVLESGIDIIAR
                     PPGKEPRSISLLSGGEQTMSALALMFSFFQAKPSPFTLLDEVDAALDEANNERYNAIV
                     QEFSQTSQFIMITHSKRSMAIANVMYGVTMQELGVSKRVSVKFEEADKLDEQLQPVTS
                     QS"
     gene            4107..>5503
                     /locus_tag="PLK08_05715"
     CDS             4107..>5503
                     /locus_tag="PLK08_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPS53032.1"
                     /translation="MFERLTERARKVMALANQEAQRFNHEYVGTEHILLGLVKEGTGV
                     GANVLKNLGVELQKVRLEVEKLVKAGPDMVTMGKLPQTPRAKKVIEFAIEEARNLHHN
                     YVGTEHLLLGLLRESDGVAAQVLLNMGLRLEDVRAEVLNLLGASTENTDDMSTDQIGR
                     PHNGLKRTKSKTPALDNFGRDLTDLAREGQLDPVIGREDEIERVVTVLCRRTKNNPVL
                     LGEAGVGKTAIVEGLAQRIIKKEVPELLHDRRIVALDLAMMVAGTKYRGQFEERIKAV
                     MNEVRRARNVILFIDEMHTLVGAGGAEGAIDAANVLKPSLSRGEIQCIGATTLDEYRK
                     YIEKDGALERRFQTIIVNPPTPEQTFKILKGLRDRYEAHHRVRISDIALQKATELSNR
                     YITGRVQPDKAIDVIDEAGARVRLKNLTTPPDLASIEDSIERLLGEKDEAVKNANYER
                     AAELRDKIENLRANR"
BASE COUNT         1498 a         1526 c         1506 g          973 t
ORIGIN      
        1 cagcagaacg atcacctggt gatgttccga aagctgccgg ataatattac gatatccgac
       61 cggttcagga cgaattttca ggtttccgag actatccgca tactcggcat gccgccgaag
      121 ccggatccga ataccggcaa agttccgggc ctgggcctta cttacagcca gatagtcagc
      181 tttctttacc gcatgaccca acaaaataac ataaaggcga aattcgtctt gcaaccgttg
      241 ccggaaatca ggagaatata cgaaatcata ccatccgtag gtcgtcatga cgtcgagaac
      301 ccataatatg gtgtgaattt gatataaaac attctggaaa cagaggatat actgggaatc
      361 agatgaaact aaaaaaactc attcttgccg gattcaagag ttttgccgac aagacggaat
      421 ttgatttcga cgacggaata agttgcgtgg tcggccctaa tggctgcggc aaaagtaacg
      481 tcgtcgacgc cgtcaagtgg gtactgggtg agcagtctgc caaaagtctc cgcggcagtg
      541 agatgatgga cgtcatattt aacggctcga gtgcccgcaa acccagcggc cgcgcgtccg
      601 tcacgctggt attcgacaat tcggacagcg tcctcaagcc cgttctgaac ggcgcaacgc
      661 aggaatgtcc cgaagtttcc gtgactcgcc ggctattccg gtccggccag agcgaatatc
      721 tcataaacaa tcagccgtcc cgcctgcgcg acattcggga gatgttcctt gataccggct
      781 tgggcgcaaa tgcctacagc attatcgaac agggcaaggt cagcgcgttt ctgcaggctg
      841 gccgcgatga gcgtcgcgcg tttttcgacg aagccgccgg catcagccgc tacaaagccc
      901 gcaaaaaaga agccatccgc aaactcgaac gtgtcgagca gaatctgttg cgcgtcatgg
      961 acatcatggc tgaggtggaa aaacgtctgc gttccataaa agtccaagcc ggcaaagccc
     1021 gcaactacca aacctacgca gaccgcctca acgaacttcg tgccctgttc ttcctgagcc
     1081 gctttcacac catgaaaaac cagcggaaaa tgcaggctga cgaactccag tccggcagcc
     1141 tctcgctgga aaccatcgag gaacagattg cccagctcga aaacgcccag cgtgccgtcg
     1201 aagttgaagc cgcgcagatt caggaaacca cccgtgagat acaggctcgc atttcaaaca
     1261 taaatgccca gatgaccacg ctgcatgagc gcgtcgagat gcagaatcgt cgcatggagg
     1321 agcttgtaga atcgttggag gttaacaatt tgcggacggc tgagctggct cagcggctgg
     1381 aggaatccgc cggcgacatc gaattgcggg aagttgagct tcagcaggtc gccgaggctg
     1441 ccgaagagtt gcggcagcag acgcaagcca ttatagacgc ctactcggcg gctgaaatgc
     1501 acatcgccga catccgtcgg gagattgaag accgccgcag cgggatgaat gaacttgaac
     1561 gtcaggaagg ccagcttaaa aacgaagccc acgccgcaag cctcaaatgt gaaagcctta
     1621 ccggcgaaaa acagcgtgtg gcagaccggc tggcggaatt gcaaaaccag attgaatcga
     1681 tagccggcgg caaaacggaa ttggaaaccc gcatgacgca ggctatcgca gcgattgaat
     1741 cgggcgaaga gcagctcggg cagcttacgg ctgtcagcga agagcttact gcgtccgaac
     1801 tgagcctcgg ccatgaatta gccgacgccc gcgaacagcg cagccgccag caaggcagaa
     1861 tgcacacgct ggaggaaatg cagaagcgca tggtcggcgt caccgaagcc acgcgtctgg
     1921 ttatccaggc gaagcaggac ggaaaatttc cagccatcca ggggatgctg ggcgatttta
     1981 tagagaccga caccgaacat gccccgctgg tcgaggcggc gttagccggc gcagaccagc
     2041 ggctcgtcgc agccacgttc gcggaagttc agcagatatc ggctcaaata gaggaaatgt
     2101 tctccgaggg cggcagtgtt gaaatcatct gtgccgacct agccggcaga cgccccgtcg
     2161 acgatgccac cgtaaactgc cctcaagccg tcggccgcgt actcgatttc ataaaatacg
     2221 aaccgtccct tgagccgctg atgtggaaca tgctcggccg aacgctcatg gtccgcacac
     2281 tcgcagatgc agcatgcgca ctggaaaaca cccctgccgg gtatcgtttc gtaacgctgg
     2341 ccggcgaagt gctcgaaccg gacggcatgg tgcgggtagg ctcagccagc cacgcagccg
     2401 gcatgattgc acgcaaaagt gaaatgcagg aaatcaccca aaacctctct gtgctcgacg
     2461 ggcaggtaga ggcgttgcag acaaggtgcg aagagacccg tcgtcagcgc gagcagcttg
     2521 aagagcagtt gcagtcagtc cgctcgacgc tatacgaggc tcgctttgaa aagagcgaat
     2581 catccagaca gatagagcaa ctgacggcaa aaataaacgc cctgaccgcc gagcagcctg
     2641 gtctggcgga aaaaatggaa catctgacga gtcagatatc gcaggcatcc cggcagcagg
     2701 ccacagccat gcagcagacg gagcaggtag ccgccgaccg cgtgcggctg gcggaaagta
     2761 tcaccgcaat ggaaacccgc ctggctgagt cgcaacaaag tatcgagcaa ctgaacgaaa
     2821 aacgcactga actcaaagta gccgtcgcag aaaacgaaca gaaacgcctt tcactccgcg
     2881 attcgctgga aagcatccgc cgtcagcgag agcagatgca ggctgagctt gaaaaagcgc
     2941 gcaacaatct tgaacttgac cgccagcgca gggagcaggc tgagcaggaa atcgcctccg
     3001 cacaggagga aggccgtcag cttgtcgggc gtctgcagga gatgcaacag gaatataacg
     3061 attcggaaga atcgcggcgg tcgctttccg aacggctggt tgaaatccgc cagcagcttg
     3121 cggaaaatcg ccagagtgaa cacgacgcca accgacttgt cggcaatatg cgggtaaagc
     3181 tcagtgaact cgacgcacat ataaccgata tcatcgagcg ggctgcacac gagatggaga
     3241 tgaacctgaa cgaactgtac ggctcatatt ctcacgacgc cgagcgtgac tgggatgcgg
     3301 tggaggctga aataaacgac ctgcgcacga aaatatcgcg tctcggcaac gtgaacatgg
     3361 acgccatctc cgaacaggac gagcttgaaa aacgcgaaga attcctccgc acgcaactag
     3421 acgacatcaa caattcgcgc aaacagcttg aagacctcat aaatcgtctc aacgtcgaaa
     3481 gccgcgagcg tttcgtcgag actttcaacg ccgttcgtga gaatttccag tcgcttttcc
     3541 gaaaactttt cggcggcggc aaagccgaca tcatgctttc cgaagaagag gacgttctgg
     3601 aaagcggcat agacatcatt gcccggccgc ccggaaaaga accgcgaagc atcagcctcc
     3661 tgagcggcgg cgaacaaacc atgagcgcac tggcactgat gttcagcttc ttccaggcaa
     3721 aacccagccc gtttaccctg ctggacgagg ttgacgcagc cctcgatgaa gcgaataacg
     3781 aacgatataa cgcaatcgtc caagaatttt cgcagaccag ccagtttata atgataacgc
     3841 actcgaaacg ttcgatggcc atcgcaaacg tcatgtacgg cgttacaatg caggaactcg
     3901 gcgtctccaa acgcgtatcc gtaaaatttg aagaagccga caaacttgac gaacaacttc
     3961 agccggtaac gtcgcaatca taacgtcgca acccacccgg cggaaaagac atttttttca
     4021 taatcaccga cgattatgct ataattcaga agtgattttt gggatttttc agactggaac
     4081 acattttttt gcggaatgac caaaatatgt ttgaacgact tacagaacgg gcacgaaaag
     4141 ttatggctct tgcgaatcag gaagcccaac ggtttaacca tgaatacgtc ggcacggaac
     4201 acatacttct gggcctggtg aaggaaggca caggcgtagg agcgaacgta ctgaagaacc
     4261 tgggcgtgga attgcagaaa gtccgtcttg aggttgaaaa gctcgtcaag gccggccccg
     4321 atatggtcac gatgggcaag ctgccgcaaa caccgcgtgc caaaaaggtt attgaattcg
     4381 ccatcgagga ggcccgcaat ctgcatcaca actacgtcgg gacggagcat ctgctcctgg
     4441 gcctgctacg cgagtctgac ggcgtggcgg cacaggtact tctgaacatg ggccttcgtc
     4501 ttgaggacgt cagggccgag gttctcaacc tgcttggcgc aagtaccgaa aatacagacg
     4561 acatgtctac cgaccagata ggccggccgc acaacggttt aaagcgtaca aagagcaaaa
     4621 cgccggccct ggataatttc gggcgtgatt tgacggacct ggcacgcgaa ggccaactgg
     4681 acccggtcat cggccgcgag gatgaaatcg agcgcgtcgt aaccgtactc tgccgccgga
     4741 cgaaaaacaa tcccgtcctg ctcggcgaag ccggcgtcgg caaaacagct atcgtcgaag
     4801 gcctcgcaca acgtatcatc aagaaggaag ttcccgaact gctccatgac cgcagaatag
     4861 tcgcactcga ccttgcgatg atggtcgcag gcacgaaata tcgcggccag ttcgaagaac
     4921 gcatcaaagc cgtcatgaac gaagtccgcc gtgcacgtaa cgtcattctt tttatagatg
     4981 aaatgcatac actcgtcggc gccggcggag cggaaggcgc catcgatgcg gccaacgttt
     5041 taaaaccgtc gctctcgcgc ggcgaaatcc agtgcatagg cgccacgacg ctggacgaat
     5101 accgcaaata tatcgaaaaa gacggcgccc ttgagcgtcg attccagact atcatagtca
     5161 atccgccgac gccggagcag acattcaaaa ttctcaaagg ccttcgcgac cgctacgagg
     5221 cacaccatcg cgtgcggatt tcggatatcg ccctgcagaa ggcaaccgag ctttccaacc
     5281 gttacataac cggccgagtt cagccggaca aggctatcga cgtaatagat gaagccggcg
     5341 cccgcgtgcg gctgaaaaat ctcaccacgc cgccggatct cgccagcata gaagacagca
     5401 tagagcggct gctcggcgaa aaagacgaag ccgtcaaaaa cgccaactac gaacgtgccg
     5461 ctgaactgcg cgacaaaata gaaaacctcc gtgcaaaccg cga
//
LOCUS       DAONFI010000141         5473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17513_length_5473_cov_3.889627, whole genome shotgun sequence.
ACCESSION   DAONFI010000141 DAONFI010000000
VERSION     DAONFI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17513_length_5473_cov_3.889627"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLK08_05720"
     CDS             complement(<1..356)
                     /locus_tag="PLK08_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53033.1"
                     /translation="MKFMSMFFMMFFGVNVITAATDGADNSKKMPQFEYDSSCVFLAM
                     DKSWMQNCEGFSISMSQAKKYEHNPVLRFEDEQNPPAYRLGHLHVTQENGIYRAWYDT
                     YYNNRFNIRYAESVDG"
     gene            871..5430
                     /gene="gltB"
                     /locus_tag="PLK08_05725"
     CDS             871..5430
                     /gene="gltB"
                     /locus_tag="PLK08_05725"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HPS53034.1"
                     /translation="MHRPPRQQGLYDPRNERDACGIGAVVNIDGHRSHQIVRQAREVL
                     LNLHHRGAAGADNATGDGAGILMQMPHEFIATKAKELKIKNLPEETYYACGNVFLPKD
                     PSLRSEMEEMFKITMEHYGLCVMGWRDLNVNPDCLGQIAKDSEPCVRQVLVNGNGLHD
                     EALECKLYVARKQAEKSILAKFGELAEEFYICSLSCRTLNYKGMFLARQLFDYYADLS
                     DESMKTAIALVHQRYSTNTFPSWKLAQPFRAVAHNGEINTISGNRQRMKIREQLMESP
                     QLCADFDEIRPVLTEGMSDSASFDEAFELFVRAGRSAPHAVMMMMPEAFGASHHMSTD
                     KRAFYEYHAALMEPWDGPAAMAFTDGRLFGATLDRNGLRPCRWVITTDNVVVLASEVG
                     VIEFPPEKISSKGRLRPGNLFLVDTQEQRIISDSEIKSKIARSKPYRRWLEENRIELR
                     GLFDAANQPIVEHQSLLKRMRAFGVTREELQTVIQPMAANGQEAVGSMGNDTPLAVLS
                     EKPKLLFNYFRQLFAQVTNPPIDPLREGTVMSLMLFMGRRRNILLETPEHCKQIKIPH
                     PILTNEDMQRLRSVKHKDVKVTTISSLFEVGSGDATANMQAALDRLVNDAEKAIDEGA
                     TILIISDRGMNEHLAAIPALLAASAVHRGLYRRRKRGKAGLVVESGEVREVMHFCLLC
                     GYGVNAINPYLAFEMLAELKAQDNVPANLDIVALVDNYIAAIKKGMLKTMSKMGISTL
                     RSYRSAQIFEAIGISKEVIDKYFTGTTSRIGGIGLEKITADAVARHNDAFRTRKPGEL
                     ELNYGGEYHYRFDGEKHLTSPEAIISLHQAVRNNDRNAYERYSKSVNDQTKAHCTLRG
                     LFKFRPGNPVPLDEVEPACEIVKRFCTGAMSHGSISKEAHECMAIAMNRIGAMSNTGE
                     GGEDPARYVLDPNGDDRNCRIKQVASARFGVTINYLAHAKEIQIKMAQGAKPGEGGQL
                     PGYKVTEEIARLRHSTPGVTLISPPPHHDIYSIEDLAQLIYDLKCSNPGVKVSIKLVS
                     EVGVGTIAAGVAKGNADEVLISGHDGGTGASPLSSIKYAGGPWELGLAETQQVLVLNR
                     LRGRIRVQTDGQLKTGRDVAIATLLGAEQYGFGTTSLLALGCVMMRKCHLGTCPVGIA
                     TQQLGLRKNFTGKPEHLIRFMFFIAEEMREIMAELGFRKVEDMIGRVDMLDVDGAVDF
                     YKQRGLDFSQIFHAVDCSDGRAVRKVTVQENKLADHLDWKIIEMLGDKLEKGQPTSVD
                     LHIRNVNRTVGTILSNRVIKKFGENGLPDNTITINLNGTAGQSLGAFLAAGITINLVG
                     DANDYVGKGLSGGRIAIKTPPNSPFVAHQNIIAGNTLLYGATEGEVFINGVVGERFCV
                     RNSGAVAVVEGCGDHGCEYMTGGITVILGQTGRNFAAGMSGGVAFVLDELQLFDTLCN
                     LDMVDLEPVHTDEDENLLKRLISDHYKWTGSSRAKWILDRWRDMVGRFVKVMPIDYRH
                     ALERMKQTDSRGTETTPATEEVFNG"
BASE COUNT         1441 a         1450 c         1414 g         1168 t
ORIGIN      
        1 attccatcga cgctttccgc ataacgaata ttaaaccggt tattgtaata cgtgtcgtac
       61 cacgccctgt atattccatt ttcctgagtc acatgcaagt gaccaagtcg gtaagccggc
      121 ggattctgct catcctcaaa tcgcaacaca gggttatgct catacttttt agcctgagac
      181 atggaaatgg aaaacccctc gcagttctgc atccatgatt tatccatcgc cagaaataca
      241 cacgatgagt catattcaaa ctggggcatt ttttttgaat tatccgcacc atcagtagct
      301 gccgttatta cgttcacgcc aaaaaacatc atgaaaaaca tggacatgaa tttcatcgca
      361 gtatcctttc agaaaacggg ttacaatatt ttaaaatact tacgcatagc atacacgctt
      421 gtacaaataa atgttatcaa aattagcaga ataatgttac caaaaatcca ttatcatgca
      481 agtttaattt tggggtcatt taaagtatag cagatggaaa accataacat tttgatttct
      541 ttagagtgat ttttgacaga aatttctttt tgtgagctgc tatgcatcac acggttcggc
      601 aaaattgccg cattgataat tggcgttttt accctataaa acagacaaaa actgaatttt
      661 tcaaaatgat taatttttaa tattagaaaa tttaattaaa aataagtaat aataatattg
      721 ccgatttcgg ataaaatatt attgtagacg aagcgtcggt caggctgcgg gtaatcggca
      781 tggccagcgc gtatgatgta tatggccccg cccaaaaggc ctgatgcggg gccgcagtaa
      841 ttacggaaat gaaccccgag gagtaagagt atgcacaggc ccccccgaca acagggattg
      901 tatgatcccc gcaatgagcg tgacgcctgc ggaattggtg cggtcgttaa cattgacggc
      961 catcgcagcc atcagatagt ccgccaggca cgcgaagttc tgttgaacct tcaccatcgc
     1021 ggcgcagccg gcgccgacaa cgccacaggc gacggagccg gcattctcat gcagatgccg
     1081 catgaattca tcgccacgaa agcaaaagaa ctcaaaataa aaaatcttcc ggaagaaaca
     1141 tattacgcct gcggcaatgt ttttctgccc aaagacccca gccttcgttc cgaaatggaa
     1201 gagatgttca aaatcaccat ggaacactat ggcctttgcg tcatgggctg gcgtgacctg
     1261 aatgtaaacc ccgactgcct gggtcagatt gcaaaagact ccgaaccatg cgtccgtcag
     1321 gttctcgtaa acggcaacgg ccttcatgac gaagccctgg aatgtaaact ctacgtcgca
     1381 cgcaaacagg cggaaaagag catacttgca aaattcggcg aactcgccga ggaattctac
     1441 atctgcagcc tgtcgtgcag aacgctcaac tataagggca tgttcctggc tcgccagctt
     1501 ttcgattatt acgccgacct ttccgacgag agcatgaaaa cggctatagc actggttcac
     1561 cagcgttaca gcacgaatac ttttccgagc tggaagcttg cccagccgtt ccgcgcggtg
     1621 gcacacaacg gcgaaataaa caccatcagc ggcaatcgtc agcggatgaa aatccgcgag
     1681 caactcatgg aatcgccgca gctttgcgcc gatttcgatg aaattcgtcc tgttctgacg
     1741 gaaggcatga gcgattcagc cagttttgac gaggcgtttg aacttttcgt tcgtgccggc
     1801 agatccgcgc cgcatgccgt aatgatgatg atgcctgaag ccttcggcgc cagccatcat
     1861 atgagtaccg acaagcgtgc gttttacgaa tatcacgctg ccctgatgga gccgtgggac
     1921 ggccctgcgg cgatggcgtt tactgacggc agacttttcg gtgccacgct tgaccgcaat
     1981 ggcttgcggc cgtgcaggtg ggtcataact acggataacg tggtcgtgct ggcaagcgag
     2041 gtgggcgtga tagaatttcc gccggaaaaa atttccagca agggccgtct gcggccgggc
     2101 aacctcttcc tggttgacac acaggaacag cgaatcatca gcgacagcga aatcaagagc
     2161 aaaatcgcac gttcaaagcc gtatcgtcgt tggctggaag aaaatcgtat agaacttcgc
     2221 ggccttttcg atgcggctaa tcagcctatc gtagaacacc agtcgctgct gaaacgcatg
     2281 cgtgctttcg gagtgacgcg ggaagaactg caaaccgtca ttcagcccat ggccgccaac
     2341 ggccaggaag ctgtaggttc aatgggtaac gataccccac tggcagtcct ttcagaaaag
     2401 cccaagctgc ttttcaatta tttccgacaa cttttcgcac aggtgaccaa tccgcccatc
     2461 gaccccctgc gggaaggtac cgtcatgtcg ctcatgctct tcatgggccg gcggcgaaac
     2521 atattgctcg aaacccccga acactgcaaa cagataaaaa ttccccatcc tatactgact
     2581 aatgaagaca tgcagcgtct tcgcagcgtc aagcacaagg atgttaaagt caccacgatt
     2641 tcatcgctgt ttgaagtcgg cagcggcgat gcgacggcaa acatgcaagc cgctcttgac
     2701 cgcctggtga atgacgcgga aaaggctatc gacgaaggtg cgacaatact cataatttcc
     2761 gaccgcggaa tgaatgagca tcttgcggcg atacccgcgt tgctggcggc gtcggcggta
     2821 caccgcggcc tttatcgcag acgcaaacgc ggcaaagccg gtcttgtcgt cgaaagcggc
     2881 gaagtccgtg aagtcatgca tttctgcctg ctgtgcggat acggcgtaaa tgccatcaat
     2941 ccgtatctgg cgttcgaaat gcttgccgaa ctgaaggctc aggataatgt gccggcaaac
     3001 ctcgatatag tggcgttggt cgataactac atcgccgcca taaagaaggg tatgctcaag
     3061 accatgtcaa aaatgggcat cagtacgctg cggagttacc gcagcgcgca gatattcgaa
     3121 gcaatcggca tctcgaagga agtcatcgac aaatatttta ccggcacgac ttcgcgaata
     3181 ggcggcatag gccttgaaaa aatcaccgcc gacgcagttg cccgtcataa tgatgccttc
     3241 cgcacgcgta agcctggcga actggagctc aattacggcg gtgaatatca ctatcgtttc
     3301 gatggcgaaa aacatctcac cagtccggaa gccatcatca gcctgcatca ggcggtacgc
     3361 aataacgacc gcaacgctta cgagcgttac tccaagtcgg taaacgacca gaccaaggcg
     3421 cactgcacgc tgcgtggact gttcaaattc cggccgggca atcctgttcc gctggacgaa
     3481 gttgagccgg cctgcgagat tgtaaagcgg ttctgcaccg gcgccatgag ccacggttca
     3541 atatcgaaag aagcccacga atgcatggcc atagccatga accgtatagg cgccatgagc
     3601 aataccggcg aaggcggaga agatccggcc cgctacgtac tcgaccccaa cggcgacgac
     3661 aggaactgcc ggataaagca ggtggcgtct gctcgtttcg gcgtaaccat aaattacctg
     3721 gctcacgcga aggagataca gataaagatg gcgcaaggcg ccaagcctgg cgaaggcggc
     3781 cagcttcccg gctataaggt cacggaagaa atcgcgcgtc tgcgtcattc cacgcctggc
     3841 gttacgctga tatcgcctcc gccgcatcat gacatatatt caatcgaaga cctggcgcag
     3901 cttatatatg acctgaaatg ttccaatcct ggcgtaaagg tatccatcaa gctggtaagt
     3961 gaagtcggcg tgggtacaat cgcagcaggc gtggcgaaag gcaatgccga cgaagtattg
     4021 atttccggcc atgacggcgg cacaggcgcg agcccgctat ccagcatcaa gtacgccggc
     4081 ggcccgtggg aactggggct tgccgaaacg caacaggtat tggttctcaa caggttgcgc
     4141 ggccgtattc gcgtccagac ggacggccaa ctcaagaccg gacgagacgt cgccatcgcc
     4201 acactgctcg gtgctgaaca gtacggattc ggcacgacgt cactgcttgc gctgggctgc
     4261 gtgatgatgc gcaagtgtca tctgggaact tgtcctgtcg gcatagcgac gcagcagctc
     4321 ggcttgcgga agaattttac cggcaagccg gagcatctca tccgcttcat gttcttcatc
     4381 gccgaagaaa tgcgcgaaat tatggctgaa ctcggcttcc gcaaggtcga ggacatgata
     4441 ggccgtgtcg atatgctcga cgtcgacgga gcggtagatt tctacaaaca gcgcggcctg
     4501 gatttcagtc agatatttca cgccgtcgac tgctccgacg gccgcgccgt ccgcaaggtc
     4561 acggtgcagg aaaacaaact cgccgaccat ctggactgga agataatcga aatgctcggc
     4621 gacaaactcg aaaaaggtca gccgacatcc gtcgacctgc acatccgcaa cgtaaatcgt
     4681 acggtcggca caatcctgtc caaccgcgtg ataaagaagt ttggcgaaaa tggtctgccg
     4741 gataacacta ttacaattaa cctgaacggc acagccggcc aaagtctcgg cgcattttta
     4801 gccgccggca tcacaatcaa tctcgtcggc gacgccaacg attacgtcgg caaaggcctt
     4861 tccggcggca gaatagcaat caaaacgccg ccgaattcgc cgttcgtggc tcatcagaac
     4921 atcatcgccg gcaataccct gctttacggc gcaaccgaag gtgaagtttt catcaacggc
     4981 gtagttggag agcggttctg cgttcgcaat tccggcgcgg ttgcggttgt ggaaggctgc
     5041 ggcgaccacg gctgcgaata catgaccggc ggcattacag tgattctggg gcagacaggc
     5101 cgaaatttcg cagccggcat gagcggcggc gtagcgttcg tactcgacga actgcaactt
     5161 ttcgacacgc tctgcaatct ggatatggtc gaccttgagc cggttcatac ggatgaagat
     5221 gagaatctgc tcaagcggct tatcagcgat cactataaat ggacaggcag ttcgagggcg
     5281 aaatggatac tcgacaggtg gcgcgatatg gtcggccgct tcgtcaaggt catgcccata
     5341 gattaccgcc acgcattgga acgcatgaaa cagaccgatt cccgcggcac ggaaaccacg
     5401 ccggcgaccg aggaggtgtt caatggctaa gcccacagga ttcattgatt acaaacgtaa
     5461 aaatccgcaa tat
//
LOCUS       DAONFI010000142         5466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17547_length_5466_cov_4.219183, whole genome shotgun sequence.
ACCESSION   DAONFI010000142 DAONFI010000000
VERSION     DAONFI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17547_length_5466_cov_4.219183"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLK08_05730"
     CDS             <1..236
                     /locus_tag="PLK08_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53035.1"
                     /translation="RQAAKEPIAGSQGFWDRFYLYFLNSSQKTCQPAKLPDFGNNHVL
                     RMGSCKKCQIKVQNMLRLTTEQTKERNINGQSR"
     gene            220..1350
                     /locus_tag="PLK08_05735"
     CDS             220..1350
                     /locus_tag="PLK08_05735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS53036.1"
                     /translation="MGNPANGKIRICEIITSLEHSGAEKVVCDLATRLDTRRFDVEVV
                     GIKGGELADWLENHGVRVHVLNIGAKREFYRVDKFLRLMHILRKGCFDIIHSHLFHAD
                     LLGRLAKPADAKYVYTIHVAEHRARPLQFQFARKFQRRCDALVAVSNAVLEHHSKMSH
                     LPPENYRVIYNGVDTQEFRFDGTARRELRARWGIAPDVTLFAFIGRFHREKGLDILFD
                     AMRLANIMGRQIRVVLAGDGPLRNMVQNFMTSETIGENVINLGFRDDIPAILSAADVL
                     VAPSRWEGFCLSIAEAMSVSLPVIATKCGGPEELVVDGQTGLLVERENRPMLAQAMIK
                     LADSPNQRSCMGMAARQRIDENFSIQRMLEQYEELFIEMLDR"
     gene            complement(1416..2519)
                     /gene="corA"
                     /locus_tag="PLK08_05740"
     CDS             complement(1416..2519)
                     /gene="corA"
                     /locus_tag="PLK08_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPS53037.1"
                     /translation="MKYNGLKGIKKFRLPLFRKGLASAKAFRPPGSVTTLPDDRQKKV
                     IIDVMDYTPKRIDEYTFDNVRDCFQFRDTSSISWINITGLTDAAQITELSDYFGINML
                     TMEDVLNTDHRPKFDEFDNYVFMIIKMLSLSRDGAMLSEQVNIIMGKNYVITFQERPG
                     DVFDPLRERLRSQKGKVRAMGMDYLMYSLADLIMDNYFVVLERLGEHIEQIDISLMAT
                     PSHEMLQKIYEFKMQLFYMRKMCWPLREVSGKILHSDSPLFTDATDIYFRDLYDHSIQ
                     VVDTSETLRDIILGITDLYMSSLSNRTNDVMRILTIIATLFIPLTFIVGVYGMNFEYM
                     PELKWKYGYPGIWAVMILNTIILLTYFKRKKWF"
     gene            complement(2669..3703)
                     /locus_tag="PLK08_05745"
     CDS             complement(2669..3703)
                     /locus_tag="PLK08_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPS53038.1"
                     /translation="MKPAVNSASRHQDWIDLLRVVSAFAVVVLHLAACVSQQPADDGW
                     LAGEIYDALTRWCVPVFVMVSGALLLDDIKRDEPAGTFYRKRLGRLGWAFAFWVTFYV
                     LFGWADAAVFNRPHDFYEVFEKILSGRPWSHLWFLYMITGLYLVVPVLRRWILPLGNA
                     SMRRLIALLLVATSINNFWAIITDRPENPFILWFADYLGYFLAGYYLRFRAKYILSSR
                     LALIFFVGSVIMSVIGMNWILAAGGSLQFARWFHHYQNPCVMIASLSMFMLAMRSSVP
                     AWAGSIAPLTFGIYLIHPAVQHIIGRLGFYPQRFMPALSIPLLAAITTTISIYAIRII
                     LKIPYLRKTV"
     gene            complement(3714..4271)
                     /gene="def"
                     /locus_tag="PLK08_05750"
     CDS             complement(3714..4271)
                     /gene="def"
                     /locus_tag="PLK08_05750"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPS53039.1"
                     /translation="MTSKEKIAHAATDIDSLAVIKYPDPRLKEVCTPIEEITDDVRAL
                     AARMKVLMFSNNGVGLAASQVGVTVRLFVTSPTFNPDDFHAYVNPEIISSEKPISEEE
                     GCLSFPGIYCKIKRPGIVTVRALSLDGEELRETHTGLAARAFQHELDHLNGVLLADRM
                     GSLARMVHRAALSELVEEYQSADRG"
     gene            complement(4358..4831)
                     /locus_tag="PLK08_05755"
     CDS             complement(4358..4831)
                     /locus_tag="PLK08_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53040.1"
                     /translation="MGKEAKNEYIPVLFADTQQAAEVYRRLLEDHEIKVLVGLDELEE
                     TAGFVPDSLVGEDGIPVLVPVENIDEAAEIITDFDNQDEFEEADDDSIEEDSGEDEDF
                     DINPAANEENIEEDEDEDEDEIIGEDMFEKDDDYGMNGDDFEELEDYDEDDFDKF"
     gene            5178..>5466
                     /locus_tag="PLK08_05760"
     CDS             5178..>5466
                     /locus_tag="PLK08_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPS53041.1"
                     /translation="MGKIKVAVVGIGNCASSLIQGINYYKDVFAKGEEIIGLAHPMLG
                     NYAPGDIDIVAAVDIDKRKVGQPLHKAVFALPNNTKVFYDDFKYDNVIVQMG"
BASE COUNT         1528 a         1345 c         1187 g         1406 t
ORIGIN      
        1 gccgccaggc tgcaaaagag ccgattgcag gcagccaagg tttttgggat aggttctacc
       61 tatatttttt aaactcatct caaaaaacct gccagcctgc aaaattgccc gatttcggca
      121 ataatcatgt tttgaggatg ggttcttgta aaaaatgtca aataaaggta caaaatatgt
      181 tacggcttac aacggagcag acaaaagaaa gaaatataaa tgggcaatcc cgctaatgga
      241 aaaatacgca tctgcgagat tatcacaagc cttgagcatt ccggcgcgga aaaggtcgtc
      301 tgcgaccttg caaccagact cgacacgcga cggtttgatg tggaggtagt cggcatcaaa
      361 ggcggcgaac tggcggattg gctggaaaat cacggcgtaa gagttcatgt attgaacatc
      421 ggcgccaagc gggagtttta tcgcgtggat aaatttttgc ggctgatgca cattctccgc
      481 aaaggctgtt ttgacataat tcactcacat ctatttcatg ccgacctgtt gggccgccta
      541 gccaagccgg ctgatgcaaa atatgtctac accatacacg tagccgaaca tcgtgcacgg
      601 ccattgcaat tccaattcgc aagaaaattc caacgtcgct gcgacgcgct cgtggcggtc
      661 tcaaacgccg tgctggaaca ccattcaaaa atgtcgcatc tgccgccgga aaattatagg
      721 gtaatctaca acggcgtcga tacgcaggaa ttccggttcg acggcacggc tcgccgtgaa
      781 cttcgcgcaa gatggggtat cgctcccgat gtaacattgt tcgctttcat cggaagattt
      841 catcgggaaa aaggccttga catacttttt gacgccatgc ggctggcaaa cattatgggc
      901 cgccaaatac gggtcgtgtt ggccggcgac ggcccgctga gaaacatggt tcagaatttc
      961 atgacgagcg aaaccatcgg tgaaaatgta atcaacctgg gttttcgaga cgacataccg
     1021 gcgatactca gcgcagccga cgtgctggtc gcaccttcac gatgggaagg attctgcctg
     1081 agcatcgcag aagccatgtc tgtcagcctg cccgtcattg ccacaaaatg cggcgggccg
     1141 gaagaactcg tggttgacgg ccagaccggc ctgctcgtag agagggaaaa ccggcctatg
     1201 cttgcgcagg cgatgataaa actcgccgac agcccaaatc agagaagctg catgggcatg
     1261 gcggcaagac aacgcattga cgaaaatttc agcatccaac gcatgcttga acaatacgaa
     1321 gaacttttta tcgaaatgct cgacagataa aaaatccgcc cgaactgact gccatcaaaa
     1381 atcctgaaaa ctatatgaca attatccaaa aattttcaaa accatttttt ccgcttaaaa
     1441 tatgtcagca aaataattgt gttcaaaatc atcacagccc agattccagg ataaccgtat
     1501 ttccacttga gttccggcat atattcgaaa ttcatgccgt atacaccgac tatgaatgtc
     1561 agcggaatga acagcgtcgc gataatagta agtatccgca tgacgtcatt tgtccgattg
     1621 ctcagcgagg acatatacag gtctgtaatg ccgagaatga tatcgcgcaa cgtttcactc
     1681 gtatcgacta cctgtatcga gtggtcgtaa agatctcgaa aataaatatc cgtcgcatca
     1741 gtaaagagcg gtgaatccga atgcagtatt ttgcctgaaa cttcacgtaa cggccagcac
     1801 attttgcgca tgtaaaacaa ctgcatcttg aattcataga ttttctgaag catctcatgg
     1861 ctcggcgtcg ccatgagcga aatatctatc tgttcaatat gctctccgag acgttcgagt
     1921 acgacaaaat aattgtccat tatcaggtcg gcaaggctat acatcagata atccatgccc
     1981 atcgcgcgga ctttgccttt ctgcgacctc agccgctcac gcaaaggatc aaacacgtcg
     2041 ccgggccttt cctgaaatgt gattacgtaa ttcttgccca tgatgatatt gacctgctcg
     2101 ctcaacatgg ccccatccct ggacaatgaa agcattttta taatcatgaa cacataattg
     2161 tcgaattcgt caaatttcgg ccgatggtcg gtatttaaaa cgtcctccat ggtcagcata
     2221 ttgatgccaa aataatcgct caactccgtt atctgtgccg cgtcggtaag gccggtaatg
     2281 tttatccagc ttattgacga cgtatcgcga aattggaaac aatcgcgaac attgtcgaac
     2341 gtatactcgt cgatacgttt aggcgtataa tccattacgt cgatgatgac ttttttctgt
     2401 ctgtcatccg ggagtgtcgt gacgcttccc ggcggcctga aagccttggc ggaggcaagc
     2461 cctttgcgaa acagcggcaa tctgaacttt tttatacctt tgagtccatt atatttcatt
     2521 ttgccgtctc ctgtaattcc tctgtttagg gcattttatc cccataataa ctgaaaggca
     2581 atatttactg ataaactgca cccgttaatt ttctaattcg gcaggtttgt aaacaggtcg
     2641 ggataagaca ggctggttaa tcttcttctc agacggtttt tctgagatac ggtattttta
     2701 gaattatgcg gatagcgtaa atggatattg ttgtagttat cgcggcaaga agcggaatag
     2761 ataacgccgg cataaatcgt tgaggataga atcccagccg gccgatgata tgctgaactg
     2821 ccggatgaat cagatatatc ccaaaagtca gcggcgctat ggaacccgcc cacgcaggaa
     2881 cgcttgaccg catggcaagc atgaacatgc tcaacgacgc aatcatcacg caaggattct
     2941 gataatgatg aaaccatctc gcaaattgca ggctgccgcc ggcagcaagt atccaattca
     3001 tccctataac gctcataatt acactgccga cgaaaaatat cagtgccagc cggctggata
     3061 gaatgtattt tgcgcggaat cgcaaataat aaccggctaa aaaatatcca aggtagtcgg
     3121 caaaccataa gatgaacgga ttttcaggcc tgtcagtaat gatagcccaa aaattattta
     3181 tcgacgtggc tacaagcagc agagctatca gccggcgcat cgaagcgttg cccagcggca
     3241 gaatccatcg ccgcaaaacc ggtacgacaa gatacaaccc tgtaatcatg taaagaaacc
     3301 acagatgcga ccatggccgg ccagacagga ttttttcaaa aacttcataa aaatcatgcg
     3361 gtcgattaaa gacggcggca tctgcccagc cgaaaagcac ataaaaagtc acccagaatg
     3421 cgaacgccca accgagtcgg cccagccgct tgcgataaaa tgttccagcc ggctcatcac
     3481 gtttaatatc gtcgagcaat agcgcgccgc tgaccatgac gaaaaccgga acgcaccacc
     3541 gtgtaagagc atcgtatatt tcgccggcta accagccgtc gtccgccggc tgctgcgaaa
     3601 cgcatgccgc caaatgcaac accaccaccg caaatgcact gacgacacga agcaggtcta
     3661 tccaatcctg atgtcgcgac gccgaattga ccgccggttt cataaaggtt tttttaacct
     3721 ctgtccgcag attgatattc ttcaaccagc tcggaaagtg ctgcgcggtg aaccattctg
     3781 gccaacgacc ccatgcggtc agccagcaga acgccgttca gatggtcaag ctcatgctga
     3841 aacgcgcgtg ccgcaaggcc tgtatgtgtt tcacgaagct cctcgccgtc aaggcttaac
     3901 gctctgacgg taacgatacc cggccgtttg attttacaat aaattccggg aaaactcagg
     3961 caaccctctt cctccgatat cggcttttcc gacgaaatta tttcaggatt gacgtaggcg
     4021 tggaaatcat cgggattgaa tgtggggctg gtaacgaaca gcctgaccgt cacgccgacc
     4081 tgtgacgctg ccaaacctac accgttattg gaaaacatca acactttcat acgtgcggca
     4141 agcgcgcgaa cgtcatccgt tatctcttct atcggtgtac atacttcttt caatcgaggg
     4201 tcgggatatt ttatcacagc caacgaatca atgtctgtcg cggcatgggc tattttttct
     4261 tttgaagtca tatcaatcct gtcaaatttt ccttgtctga aaacaatgct tgaatttaca
     4321 atggcatgat atattttgaa agttgtttct tcatcttcta aaacttgtca aaatcatctt
     4381 catcataatc ctctaattct tcaaaatcgt caccattcat tccgtagtcg tcgtctttct
     4441 cgaacatatc ttcaccaata atttcatctt catcctcgtc ttcatcttct tcgatatttt
     4501 cctcattagc tgcaggattt atatcaaaat cttcatcttc tccagaatct tcctcaatgc
     4561 tgtcgtcgtc tgcttcttca aattcgtcct gattatcaaa atccgtgatt atttctgccg
     4621 cctcatcaat attttcgaca ggtacaagaa ccggtatgcc gtcttcgcct acaaggctgt
     4681 caggaacaaa accggctgtc tcttccagct cgtccaaccc aaccaggact ttaatttcgt
     4741 ggtcttccaa aagacggcga tacacctccg cagcctgttg tgtatctgca aacaatacgg
     4801 gaatatactc gttttttgct tctttaccca taatacacct cgaacattga agtcggcatt
     4861 attaaattgt atgcgattct gccggtatat tttgtaatat ttcgttaacg atatattatg
     4921 cgggcagttg tcgcattcat acagagtttt tgttcagccg tcgatttttc agggctattc
     4981 gaaaaatacg ctcaagtagt ataaaactca aatttccctg aagttcaaca aaaaatcccg
     5041 attaaacggc atgaataaat aatttttttt gcacttattg acgctaggtt caaatgtgca
     5101 taaaatctta gttttcaaag gtacctgcat tttaacgatt ctgttgtttt aacccggttt
     5161 tggaaggaaa gttcaaaatg ggaaaaataa aagtcgccgt agtcggaatc ggcaattgcg
     5221 cttcgtctct cattcaaggc atcaattact ataaagacgt ttttgcaaaa ggcgaagaaa
     5281 tcataggcct ggctcatccg atgttgggca attatgcgcc tggcgatatc gacatcgtcg
     5341 cagccgtcga catcgataag cgcaaagtcg gccagccgtt gcacaaagcg gtctttgccc
     5401 taccaaacaa cacgaaagtt ttttatgacg atttcaaata cgataacgtc atcgtccaga
     5461 tgggca
//
LOCUS       DAONFI010000143         5438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17685_length_5438_cov_4.120379, whole genome shotgun sequence.
ACCESSION   DAONFI010000143 DAONFI010000000
VERSION     DAONFI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17685_length_5438_cov_4.120379"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLK08_05765"
     CDS             <1..469
                     /locus_tag="PLK08_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53042.1"
                     /translation="LVPGWLYMQSHKNGKTYAVDYYARYIIPIAETKWALSHNNELIA
                     QIASKDKVKIHKLELPQLPPPPKPVKPEEYWIIFRDKEKEQQERDRKAAEDAKAAADA
                     AASQPASAPATQPAKTHDNGKSSKHKDSQPTPKLQEIKKSPPLILPPQTPIEI"
     gene            582..1244
                     /gene="nth"
                     /locus_tag="PLK08_05770"
     CDS             582..1244
                     /gene="nth"
                     /locus_tag="PLK08_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPS53043.1"
                     /translation="MPAESSENMKKRADKVFAELKKLYPDAGCSLKHVSPLELLVATI
                     LSAQCTDERVNIVTADLFKKYRTARDYACAKQEELENDIRSTGFYRNKAKNIRAAAAL
                     LVSDFGGKVPQTMEELLRLPGVARKTANVVLGNAFGINSGFVVDTHVKRLTGRLGFTK
                     HTDPIKIEQDMMTLTPQKDWAIDAHLLIYHGRNYCMARNPNCRQCPLGIKKLCPSADM
                     AK"
     gene            complement(1326..2186)
                     /locus_tag="PLK08_05775"
     CDS             complement(1326..2186)
                     /locus_tag="PLK08_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53044.1"
                     /translation="MMFNERAIAMRKIFAIFMILAFVSGVSSADVGRLTDAQKRELLA
                     EAQSEYDRGTSMEAASPDEARAAYTNAAEKFQVVVSSLSRPGGWLYYDLGNAYLKSGR
                     LGLAIANYKRAAQYIGGDSRLTSNLDYARGMCASQLPQPRSQGWSGALAAMNRRVSAT
                     VRLWIGIGGWCVMWLGILGKMFIRRRRWRMLILSGAAVCVLTAASLAWEYSVLADDYQ
                     GVITARETVIRKGNGIGYAPLFKEPLCEGAEFRVLDERRGWLEVQLGDGKTGWIPKTA
                     AELIAPRKMF"
     gene            complement(2183..3958)
                     /locus_tag="PLK08_05780"
     CDS             complement(2183..3958)
                     /locus_tag="PLK08_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPS53045.1"
                     /translation="MQKFFGAVSFILLAFVCVAAQAGSVEFVLSSRETTVGIPVHGQL
                     TIENATDYEQPTLPDVDGLTIKLSPFPSTSSFTQIINGKTTSKQIVSFACEVTPVRAG
                     EFTIPSFDVIVDGRKCSTSPVTLAVNKTQGGDSLLFVEISGDINKAYVGQQMTLTLKI
                     YLKPFYSTRFRRQLTEREMASSIDFNRSTWGLFTDTIHELRSLPQGTEVLRKDSEGNS
                     RNYYLYEFECKYDTNKPGRLLGENINIIVNYPVKLVPVNDFFNDRLRVESRPVSAQAS
                     VSDIEILPVPLKGRPSLFNGAVGNYELTTKAVPAEVSVGDPITLTLIVSGIGRLNELP
                     APELRKIPELIQDFKVPNEPLAGVVKDNAKYFVTSIRPVSDKVAMIPSIPLTFFDPTT
                     GKFSTVSSEPIRIKVKPVEKFTLRKVVSAAPVEKHVDRLTETNIGIYANETDVTKLLP
                     SDEYYVDGVELVIFAAGPAVVLLGLLLKLYMWLMGSESAKRRRNAGRAARGVLSSAET
                     ADDVEAAVLRFVADRACKPVAGMTRGEAVAEMKARRWPQNVIDAADEFLGRCEHLRYS
                     GGGVAALDELKKQARKCLREMDRQK"
     gene            complement(4109..>5438)
                     /locus_tag="PLK08_05785"
     CDS             complement(4109..>5438)
                     /locus_tag="PLK08_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53046.1"
                     /translation="KLLEQIAITGGGAYIPAGTARLDMADVYNKYIDPMQEREFEQTR
                     VSTYIPRYQWFLGLSLILFLIEAFILGKRNGGAGKFFRFTKNSAVCLAIGVMCLACGA
                     TKADVSPQDAVREANRELQAGQFDKALKTYEQVKVNEPLDAQRLIYNQAVARYRKGEL
                     QKARQLLKQAAEQGNKPLEAMARYNIGNCFYGDAISLAKQKKFKESIELLDKSIAEYR
                     KSLAVQQADNDARANIELAELLKKQLKDEQKKQQQQQKQQNKQNQQQNKNQQQNKDQN
                     KQKQQQQQNKNQQQQNQSNKDRQDKNKQNRQQNQQDKNKQQQDKNRQQQNQDKQQNRQ
                     DNKEQQNKQPQNKDKQQQNGQQNPQQQQNNQPQQQQNQPEPQSGEKDEKQKSQAGGSA
                     SAQPQQAKPKELSDEEAARLLQGIRDRNLKRRIDKYRKMQAEQAPVDKDW"
BASE COUNT         1266 a         1519 c         1269 g         1384 t
ORIGIN      
        1 cctcgtgcca ggctggctgt atatgcagtc gcataaaaac ggcaaaacct acgccgtgga
       61 ctattatgcc agatatatca tacccatcgc cgaaacaaaa tgggcactga gtcacaataa
      121 cgaattaatc gcacaaattg cgtccaaaga caaggttaaa atccacaagc tcgaactgcc
      181 gcaacttccg ccgccgccta aacccgtcaa gccggaagag tactggataa tcttccgcga
      241 taaggaaaaa gaacagcagg aacgtgaccg aaaggctgcc gaagatgcaa aagccgccgc
      301 agatgctgca gcctcacaac cggcctccgc accagcaacg caaccggcta aaacccacga
      361 taacggcaaa tcctcgaaac ataaagacag tcagccgaca cctaaattac aggaaattaa
      421 aaaatcgccg cccctaatat taccgccgca gacgccgata gaaatatgag tcttgtccag
      481 ccaacatggt aatgtaaacc ggtgctaaaa aatttagcgt ggttcgcaaa aaatttcttt
      541 ctataatcgt tctattttcc aaatgaggta cgaaacgaca tatgcccgct gaatcatcgg
      601 aaaacatgaa aaaacgtgcc gataaggttt ttgccgaatt gaaaaaactc tatcccgacg
      661 ccggatgttc gctgaaacat gtctcgccgc tggaattgct ggtagccacg attctttcgg
      721 cacaatgtac tgacgagcgc gtcaatatag tgacggcaga cctttttaag aaatatcgca
      781 ccgcccgcga ttatgcctgc gcaaaacagg aagaactgga aaacgacatc cgttccacag
      841 gtttttaccg aaataaagcc aaaaatatcc gcgccgcagc cgctcttctc gtttcagact
      901 tcggcggaaa agtgccgcag acaatggaag aactcctccg tctgccaggc gtagcccgca
      961 agacagccaa cgtagtgctc ggcaacgctt tcgggataaa ctcaggattt gtggtcgaca
     1021 cgcacgtcaa gagactgacc ggccgcctcg gattcaccaa acataccgac ccgattaaaa
     1081 ttgagcagga tatgatgact cttacgccgc agaaagactg ggcgatagat gcacatctgc
     1141 ttatatatca cggcagaaac tactgcatgg cccgaaatcc gaactgccgg caatgcccgc
     1201 ttggaataaa aaagctctgc ccatcagccg acatggcaaa atgacgctca gatttttgcg
     1261 atagataccg gtttttcaga tttttcagac tttttagatt tttaaaaaaa atttagagaa
     1321 tcgttttaaa acattttgcg aggtgcaata agttcggcgg ctgtcttggg tatccagccg
     1381 gttttgccgt cgccaagttg cacttccaac cagccgcgac gctcgtccaa cacgcggaat
     1441 tccgcacctt cgcacagcgg ctctttgaaa agcggtgcat atccaatgcc gttgccctta
     1501 cgaattacgg tttctctggc agtaatcaca ccctgataat cgtcggctaa tacggaatac
     1561 tcccatgcca gcgaggcagc cgtcaaaacg cagacggctg ctccggaaag tatcaacatc
     1621 cgccaccgtc tgcgtctgat gaacattttt cccaatatgc ccagccacat tacacaccat
     1681 ccgccgatgc caatccataa tcgcacagta gcacttactc gccgattcat ggcagccaac
     1741 gctccgctcc agccctggct gcgcggctga ggcagttgcg acgcacacat gccgcgcgcg
     1801 taatccagat tcgacgtcag ccgcgaatct ccgccgatat actgcgccgc ccgcttgtaa
     1861 ttcgctatcg ccaacccaag cctgccggac ttcaggtacg cattgccgag gtcgtaatac
     1921 agccatccgc caggccggct gagcgatgat acaactacct ggaatttttc ggcagcatta
     1981 gtgtatgccg cgcgagcctc gtccggcgag gccgcctcca tcgaagtacc acggtcatac
     2041 tcgctctgcg cctcggcgag aagttcccgt ttctgggcgt cggtcaggcg gccgacatca
     2101 gccgacgaca cccctgaaac gaatgccaaa atcatgaata tcgcaaatat ttttctcatg
     2161 gcgattgccc gttcgttaaa catcattttt gtctgtccat ttccctcagg catttacgag
     2221 cctgcttttt gagttcatcc agcgccgcga ctccgccgcc tgaatatcgc agatgttcac
     2281 accggcccag aaattcgtca gccgcgtcga tgacattctg cggccaccgg cgagctttca
     2341 tttctgcgac ggcttcgccg cgggtcatgc ctgcgacagg cttacacgcg cggtctgcca
     2401 cgaaacgcag aacggcggct tccacgtcgt cagccgtttc cgccgacgac agcacgccgc
     2461 gagcagctct acccgcgttg cgacgacgct tcgcagactc gctgcccatc agccacatat
     2521 aaagtttcaa cagcaaaccc agcaacacca ccgccggacc ggcggcaaat atgactaact
     2581 ccacaccatc cacgtaatat tcgtccgacg gcagaagttt cgtcacgtcc gtctcattgg
     2641 cgtaaatgcc tatattcgtc tcggtaagcc tgtcgacatg tttttcgacc ggcgcggcac
     2701 tgacgacttt ccggagcgtg aatttctcga ccggcttgac ctttatcctt atcggctcac
     2761 tgctgacggt cgaaaatttg cccgtcgtcg ggtcaaagaa ggtcaacgga atcgacggta
     2821 tcatggcgac cttatcgctg accggccgta tgctggtcac gaaatatttt gcattgtctt
     2881 tcacgacgcc ggctaacggc tcgttcggca ctttgaaatc ctgaatcagt tccggaattt
     2941 tccgaagttc cggcgcaggc agctcgttca ggcggccaat gccgcttact atcagagtca
     3001 gcgtaatcgg gtcgccaacg ctcacttccg caggcacggc tttcgtagtc agttcgtaat
     3061 tgccgacggc gccgttaaac agcgacggac ggcccttgag cggcacaggc agaatctcaa
     3121 tatcgctcac ggaagcctgc gcacttacgg gccgactttc aacccgcagc cggtcattga
     3181 agaaatcatt aaccggcaca agttttacgg gataattcac gataatgttg atattttcgc
     3241 ccaaaagtct gccgggctta tttgtatcgt acttgcattc gaattcatac aggtaatagt
     3301 tgcgggaatt accctcggaa tccttgcgaa gaacttccgt accctgcggc agagaacgaa
     3361 gctcgtggat ggtatcggta aaaaggcccc acgtactgcg gttgaaatct atcgaggaag
     3421 ccatttcgcg ttcggtcaac tgacgacgga atcgcgtgga gtaaaacggc ttgagataga
     3481 ttttcagggt aagcgtcatc tgctggccga cgtaggcctt attgatgtca ccggaaattt
     3541 ccacgaacaa cagcgaatcg ccaccctgcg ttttgttgac cgccagggtc accggcgacg
     3601 tactgcattt cctgccgtcg actatgacgt caaacgaagg gatggtaaat tctcccgcac
     3661 gtacgggcgt cacttcgcag gcaaacgaca ctatctgttt cgaagtcgtc ttgccgttga
     3721 ttatctgcgt aaacgaagac gtactgggaa acggcgagag ttttatggtc aatccgtcca
     3781 catccggcag cgtcggctgt tcataatcgg tcgcattctc tatggtaagc tggccgtgta
     3841 caggtatgcc gaccgtcgtt tccctggatg aaagcacaaa ttcaacgcta cccgcctgcg
     3901 ccgccacgca cacgaatgcc aacaatatga aactgactgc cccaaaaaac ttttgcatca
     3961 cgaaacctca aaaccgcata ctgcggcaaa tttcaatttc cagtcacaaa cctttcgaaa
     4021 atccgtcaat caaaatcgac gacaaacaca acgcgacaca gattacagcc agtcgaaagc
     4081 cgctcggcat acatacatta tcgacgcatc accaatcctt gtcaaccggt gcctgttctg
     4141 cctgcatttt acggtatttg tcgatgcgac gtttcagatt tctgtcgcga ataccctgga
     4201 ggagacgcgc ggcttcttca tcgctgagtt ctttaggctt tgcctgctgc ggctgggcag
     4261 aagcgctgcc gccggcttgt gatttctgtt tttcatcttt ttcgcccgat tgtggttcag
     4321 gctgattctg ttgctgttgc ggctgattgt tctgctgttg ctgcggattc tgctggccgt
     4381 tctgctgttg tttatcctta ttttgcggct gtttattctg ttgctcttta ttgtcctggc
     4441 gattctgttg tttatcctgg ttctgctgtt gccgattttt atcttgctgc tgtttatttt
     4501 tatcctgctg attctgttgc cggttctgtt tgtttttatc ctgcctgtct ttattgctct
     4561 gattctgctg ttgctggttc ttgttttgtt gctgttgttg tttctgcttg ttctggtctt
     4621 tattttgttg ctgattttta ttctgctgct gattctgctt gttttgttgt ttctgttgct
     4681 gctgctgttt tttctgttca tccttcaact gttttttgag gagttcggca agttcgatat
     4741 tggctctcgc atcattgtcg gcttgttgca cggcaagcga tttgcgatac tccgcaatcg
     4801 atttatccag cagttcaatg ctttccttga attttttctg cttggccagg cttatcgcat
     4861 cgccataaaa acagttgccg atattgtacc tcgccatagc ttccagcggc ttattgccct
     4921 gttcagccgc ctgttttaaa agttgtcgtg ccttttggag ttctcccttc cggtagcgtg
     4981 cgacagcctg attgtaaata agccgttgcg cgtccagcgg ctcattgacc ttcacctgtt
     5041 cataggtttt gagggctttg tcgaattggc cggcttgcaa ttccctgttt gcttccctga
     5101 cggcatcctg cggagagacg tcagccttcg tcgcgccaca ggccagacac atcacgccta
     5161 tcgccagaca cactgccgaa tttttcgtaa atctgaaaaa ttttcccgca ccgccgttgc
     5221 gtttgcccag tatgaacgcc tctataagga acagaatcag gctcaggcct aaaaaccact
     5281 gatatctcgg tatataagtg ctgacccgcg tctgttcgaa ttcgcgttcc tgcatcgggt
     5341 cgatgtattt gttataaacg tcggccatgt caaggcgagc cgttcccgcc ggaatatacg
     5401 caccgccgcc ggtgattgca atctgctcca gcagtttg
//
LOCUS       DAONFI010000144         5415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17797_length_5415_cov_4.992724, whole genome shotgun sequence.
ACCESSION   DAONFI010000144 DAONFI010000000
VERSION     DAONFI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17797_length_5415_cov_4.992724"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2449
                     /locus_tag="PLK08_05790"
     CDS             <1..2449
                     /locus_tag="PLK08_05790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPS53047.1"
                     /translation="IQEAEGPVEPRAMLLILFRLASALHDVHSAGIVHRDIKPSNIVM
                     STNGLRPTIIDFGVATTTDSNASMPLATTKYLAPEIYAGNGEVDGRADLYSLGFIAYE
                     MLIGREKFNEIFEDIVRDKHSQALRWMKWHGNPAVTAPRLHDVLPSIPVPLSNVVARL
                     MEKDPNRRFKSAEEFAIALRGNFAGKTAKKSAPAKVGQHKGKGGMISQMANMEIDSMQ
                     QQGMDVPQFSKSAAAAASHDGGVILDETPTAPLPAGPMSRRKKIMLISLASAAAVFLV
                     ALLSVFLMNAHYKSEHYNEMSRQVYIEGANLFTQGRFGSAMEKFDEVCKEFPHSSSSD
                     RARLLGIMCKINIDIDGADWAAAQLQENTAEEFLNKLQTTSNNEEFTNWLRKRKTDLD
                     ALKRYRYSSNIFYNATNAARKMIKNPIKVADFDQALNNLSSDLAIGDVNLTEGQEKAL
                     AELKNCISRERVRFTFSQLLRDGDTAEKLGQYDVARQAYQKALALFDGSEPGVKLLDR
                     GQYENMLATASQKLKELMSSQTLAKLIEAINRAHTNNDDKALKSAITQALKMPDLDAK
                     TKAQYTKELRLINAREKLSKAKRLIAEGKTSAARETLVEADKLCPKNAEISDLISKLD
                     AAAKRETIINDASAEFTAGRYSKAMNLYVAAQKISEDSDTASRIIDCRFNLAVENAKQ
                     LAQEKKYDEARAEYTNAKQIKPAAAAQVDAMLVMLDDQQKYQNLVEMGDKEMKQNRFQ
                     EALKYYDDASKIQATPEIQKRVQLTRYKKDIYLGKRALEEDDEKTARYHFKRALQFLD
                     TPEARELLNKVGGANED"
     gene            2455..3234
                     /locus_tag="PLK08_05795"
     CDS             2455..3234
                     /locus_tag="PLK08_05795"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HPS53048.1"
                     /translation="MAEHEQQAIGVQNKWLILIALVLGILAAAVYNLHIKAIRDEYEG
                     NSVKIIELRRDLSAGDRLRSDCIEACEIPATEAARKSFGNVIKWSQKDMLLVEGGKRV
                     NQPVRKGQFLLWDHITGREGTNPSNRISDGMVLKSIEFDKSQSLGDALSAGDRVNLCG
                     LFSLNGQPLQSYRIIEGVRVLNIGGKGAAVEGKSVGQSYNRITVEVPEEVSLRLDNVL
                     THMTGQIQLELRPYSAPAPTDININPLLGSLATQAVKGRTR"
     gene            3261..3527
                     /locus_tag="PLK08_05800"
                     /pseudo
     CDS             3261..3527
                     /locus_tag="PLK08_05800"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
     gene            4065..5156
                     /locus_tag="PLK08_05805"
                     /pseudo
     CDS             4065..5156
                     /locus_tag="PLK08_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
     gene            5146..>5415
                     /locus_tag="PLK08_05810"
     CDS             5146..>5415
                     /locus_tag="PLK08_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53049.1"
                     /translation="MNAELIYTLLILIFSALCVFMGLNPIISLIRTVEKQNSFILQNQ
                     LLLDVSARTVTILTAVSSLVFGILASSLTNWSLIAFLLAAGGGLML"
BASE COUNT         1485 a         1518 c         1409 g         1003 t
ORIGIN      
        1 catacaggaa gcggaaggcc ccgtcgaacc ccgcgcgatg ctgctgatac ttttccgcct
       61 tgcaagtgcg ctgcacgacg tgcattccgc gggcatagtt catcgcgaca tcaagccgag
      121 caacatcgtc atgtccacca acggccttcg gcccacaata atagatttcg gcgtggcaac
      181 caccaccgac agcaatgcgt cgatgccgct agccaccaca aaatacctgg cgccggagat
      241 atacgccggc aacggagaag tcgacggacg tgccgactta tactcgctgg gcttcatagc
      301 ctacgaaatg ctcataggcc gtgaaaaatt caacgaaatt ttcgaagaca tcgttcgcga
      361 caaacacagc caggcgttgc gatggatgaa atggcacggc aatccagccg tcaccgcacc
      421 gcgtctgcac gacgttctgc cgtccatccc cgtacctctc agcaacgtgg tagccaggct
      481 gatggagaaa gacccgaaca ggcgtttcaa atcggcggag gaattcgcaa tcgcgttgcg
      541 gggcaatttc gccggcaaaa ctgcaaagaa atccgccccc gccaaagtcg gccaacacaa
      601 aggcaagggc ggcatgatat cgcagatggc gaacatggaa atagactcca tgcaacagca
      661 gggcatggat gtgccgcaat tctcgaaatc agccgcagcc gccgcatcac atgacggcgg
      721 cgtaatactt gacgaaacgc ccacagcccc cctgccggct ggcccgatga gccgcagaaa
      781 aaaaatcatg ctgatatcgc tcgcttccgc ggcggcggtg ttcctggtgg cactgctgag
      841 cgtatttctg atgaacgctc attacaaatc ggaacactac aacgaaatgt cgcgacaggt
      901 ttacatcgaa ggcgcaaatc tcttcacgca gggccgcttc ggctcggcga tggagaaatt
      961 cgacgaagtc tgcaaggaat ttccgcattc atccagcagc gacagagccc ggctgctcgg
     1021 tatcatgtgc aaaatcaata tcgacatcga cggcgccgac tgggccgcag cacagttgca
     1081 ggaaaacaca gcagaggaat ttttgaacaa gctccagacg acctccaaca acgaagaatt
     1141 caccaactgg ctgcgcaagc gcaagaccga cctggatgcc ctcaaacgtt accgttacag
     1201 ttcgaatatt ttttacaacg ccacgaatgc cgctcgcaag atgataaaaa atcccatcaa
     1261 ggtcgcggac tttgaccagg ctctcaacaa tctgagctcc gacctggcca tcggcgatgt
     1321 aaatctgacc gaaggccagg aaaaagccct cgccgagctg aaaaactgca tctcgcggga
     1381 aagggtcagg ttcacgttca gccagttgct gcgcgacggc gatacggcgg aaaaactcgg
     1441 ccaatacgac gtcgccaggc aggcatatca aaaggccctg gcacttttcg acggcagtga
     1501 gcctggcgta aaacttctcg acaggggcca atatgagaat atgctcgcaa ccgcgtcgca
     1561 aaaactgaaa gagttgatgt ccagccagac gctcgcaaaa ctcatcgaag ccataaacag
     1621 ggctcatacc aacaatgacg acaaggcctt gaaatcagcc ataacgcagg cattgaagat
     1681 gcccgacctg gacgcaaaaa ccaaggctca atacaccaag gaattgcggc ttatcaatgc
     1741 tcgcgaaaaa ctctcgaagg ctaagcgtct gatagccgag ggaaaaactt cagccgcgcg
     1801 agagacgctc gtcgaagccg ataaactctg cccgaaaaat gcggaaatat cggatttaat
     1861 ctcaaaactc gacgcagccg ccaaacgcga aacaatcatc aatgacgcca gcgcagaatt
     1921 caccgccggc agatattcaa aggctatgaa cctatacgtc gccgcgcaga aaatttccga
     1981 agattccgac acggccagcc gcataatcga ctgccgcttc aatctggccg tcgaaaacgc
     2041 caaacaactc gcgcaagaga aaaaatacga cgaagcccgc gccgaatata ccaacgccaa
     2101 gcagataaaa cctgccgccg cggcccaggt cgacgccatg ctcgtaatgc tggacgacca
     2161 gcagaaatat cagaatctcg tcgagatggg cgacaaggag atgaagcaga accgtttcca
     2221 ggaggcgttg aagtattatg acgatgcgtc gaaaatacag gctactcccg agattcagaa
     2281 gagggtacag cttacccgtt acaaaaagga catttacctg ggcaaacgtg cccttgaaga
     2341 agatgacgaa aagacggcac gataccattt caagcgtgcg ttgcagtttc tggacactcc
     2401 cgaagccaga gaactgctca ataaagtcgg cggagcaaac gaggattaat aatcatggct
     2461 gaacacgaac aacaggccat aggcgttcag aacaagtggc tgatactcat agcattggtt
     2521 ctgggcattc tggcggctgc ggtatataac ctgcatatca aggctattcg cgacgaatac
     2581 gagggcaact cggtaaaaat aatcgaactt cgtcgcgatc tttccgctgg cgacaggctt
     2641 cgcagcgatt gtatcgaggc atgtgaaata ccagccaccg aagcggctcg taaatctttc
     2701 ggcaacgtca taaaatggtc gcagaaagac atgctgctgg tcgagggcgg caagcgcgtc
     2761 aatcagcccg tccgcaaagg ccagttcctg ttgtgggacc acatcaccgg ccgcgaaggc
     2821 acgaatccgt caaacaggat ttccgacggc atggtgctca agagcataga attcgacaaa
     2881 agccagtcgc tcggcgacgc cctttccgcc ggcgaccgcg taaatctctg cggcctgttt
     2941 tcactcaacg gccagccgct gcaatcgtac cggataatcg agggcgtacg cgttctgaac
     3001 atcggcggca aaggcgctgc cgtcgaaggc aaatcagtcg gccagagtta taatcgcata
     3061 acagtggaag ttccggaaga agtctcgctg cggctggaca acgtcctgac gcacatgacc
     3121 ggccagatac agctggaatt gcggccctat tcggcgccag ccccgacgga tataaacata
     3181 aatccgctgc tcggctctct ggcgacgcag gctgtcaaag gcagaacccg ctaagttata
     3241 tagcaaaacg gagactacag gtggaaatct gggtttacaa ccaattcaag gacgaacgca
     3301 ggacgctgaa agttgccgac cattcggtga cgttcggccg cgatgagtcg tgcgatatcg
     3361 ttttgcccag ccagtttgtc tcgcgaaagc acggcaggat ttttttcgag ggcggcagct
     3421 attatatcga gataaccggc cgcggcggcg cggtggtggc taaccaggaa gtgcaccctt
     3481 cgcaaaaagt caaaatcgaa ttcggcgacg aaatccgcat cggcgaattc agcatataca
     3541 tgatgcagcc gactgccagg cgagtcgccg caaaatccgc cgtcgattcg ccgcgaaaac
     3601 gggtcatcga cctcgaacag gccatgcacg ccgaactgct cgaacagctc aatctgcgcg
     3661 tcaccggcca atcgggagtg tcggacgcaa acgtagtggc gctgataaaa cgccagttgc
     3721 agaatataat ctccgcccac ttcaacgaaa cagacgatga aatgcggcag tacgtcgtac
     3781 gggaatatct ctggcgaagc gtggtcacgg aactggctcg ccgttctacg ggcaaactca
     3841 tgtacagcta cggctttgag gataccgacc tcacagaccc cggccaggaa gaaataatcg
     3901 ccagaatcat ttccgacata ataagctggt ttccgctaaa acttaaaccg agctatttta
     3961 aggaaaacat cgccgccatc gaagacggct gggaacatca actccagaaa atttctccga
     4021 aattgacgcc gcaactgcag tcatacatca cgcggcggat gttcagcaag gagatagagg
     4081 acgtcgtact cggttacggc ccgctgcagg acctgttgga aatgcccaac gtaaatgaaa
     4141 tcatggtcgt cggccgtgac cgcatatata ttgaaaaaga cggctcattg cagaacacgg
     4201 gcagaagttt cttctcggac gcgatagttg aaagcatcat cgagcggata atcacgcccg
     4261 tcggccgccg aatagaccgc tcgacaccgc tcgtcgacgc ccgcctgccc gatgggtcgc
     4321 gcgtcaacgc agtaatcaac ccgctgagcc tttcagggcc gacgctgacg atacgaaaat
     4381 tcgcgcagat accttttact atggacgacc tcatcgagcg aaaaacgctt acgccacccg
     4441 tggcgagttt tttgagagcc tgcgtcctcg gccgaaagaa catactcatc tccggcggca
     4501 caggcagcgg caaaaccacc acgctgaacg tacttagctc gttcatcccg caaagcgagc
     4561 gtatcataac gatagaagac tcggctgaac tgcaactcaa gcaggagcat ctggtgcgac
     4621 tcgaaaccaa gcccgcgaac atagagggca aaggcgcata caccatacgc gatttagttc
     4681 gcaacgcgct gcgaatgcgg ccggacaggc tgatagtcgg cgaagtacgc ggccccgaag
     4741 cgctggacat gttgcaggca atgaacaccg gccacgacgg ctcactatcg acaattcacg
     4801 ccaacacgcc ggacgacacc ctgctgcgtc tggagacgat ggtactgatg gctgtcgata
     4861 tgccgatacg cgcaatacgc gaacagattg ccgccgcact ggacctcatc gtgcagataa
     4921 cccgactggc tgacggctca cgcagagtaa cgcacgtgag cgaagttttc ggaatagaag
     4981 aagagaccgg cagaatactc acggaagata ttttcgcagt ccgcagcacg gatatagatg
     5041 ccgccaactt cggcagactc aaacatacgg gctatattcc gcacttcgcc gaagaactcc
     5101 tgagacggga gttcctgaca ctggatgttt tcaccggata tacagatgaa cgctgaactc
     5161 atttatacac tgttgatact gatattttcg gcactgtgtg ttttcatggg cctgaacccg
     5221 attatctcgc tcatcagaac agtcgaaaag cagaattcgt tcattctgca aaatcagttg
     5281 ctgttggacg tttcagcccg cacggttacg attctgacgg cagtttcgtc gctggttttc
     5341 ggcatattgg cgtcatcatt aaccaactgg agcctgattg cgtttctgct tgccgccggc
     5401 ggcggattga tgttg
//
LOCUS       DAONFI010000145         5415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17798_length_5415_cov_4.959328, whole genome shotgun sequence.
ACCESSION   DAONFI010000145 DAONFI010000000
VERSION     DAONFI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17798_length_5415_cov_4.959328"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2534
                     /locus_tag="PLK08_05815"
     CDS             <1..2534
                     /locus_tag="PLK08_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPS53050.1"
                     /translation="QVYGRTPDELSQNIEDFLSIVVPEDKSRVSNVIQDAKNGRTFSI
                     EYSLFMPDGMVRTVFERGEPEYDEAGQLIAINGTVFDVTERVLAEQAIRSSEEKARVL
                     MNSTPDFFVLCNADGIIVDCNSAFVKFRGVARSKLIGQYLFDFYIGKDREQKRRLFMK
                     VVDTFQPVHFEDAMGNVFLSTILSPIKDDAGHVSMVGIYSRNLTEYKKIQNALKDSEE
                     QLRVTLASIGDGVIATDANSNVILMNSVAENLTGWKFDDALGKPLTAVFQILSGHTRL
                     PIVNPVGDVLATGRIRQLANGILLISRDGREYQISDSAAPIRDENGNIRGVVLNFRNM
                     TLEYQRRKILQRTQFSIDHAPLEIYLLDKDGRYVYANNLAKANFGLTDTSITGTTIFD
                     INPGIDRQWWEKLKRDVMLQGVVHCESTHRTADGHTYPVDVRYYRVNLDDVDYLCIFA
                     IDISSKKQAEEQRQMILEAAGDGIFGVDAFGNIIFMNPAAYKLFGYEDDELVGRPLKE
                     ILCSNDSEDSAYVMELCPMVLAYTCGIESHIDDEMLPRKDGSRFPAEYIARPLKRKNE
                     IIGAVVTLRDMTEKKKTESALRASEEQYRSLFESSRDAVIIMDEAGTFLNANDSAVKL
                     FGYRTISELQGLNPMDTSPKTQPEGLASETLAKIRFKQALNEGGSFFEWQHKRKDGSE
                     FLGEVSLNPVMYRGHRAMQAVLRDITARKEMEQKLRQSLSEIESIFENSAVGILYLSG
                     TERKIQRANKRFLELLGYEENEVLGQSVIMFHVSKAKFKEFGKYYKENFIKGEMVRTE
                     IPLKRKDGTVIQCIISGKAIDPPDLSKGVIWIVDLSSLIEQGNCK"
     gene            2630..3292
                     /locus_tag="PLK08_05820"
     CDS             2630..3292
                     /locus_tag="PLK08_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPS53051.1"
                     /translation="MKAILFDFDGVIVDSEPLHFESFCAVLKPYGINLDRDYYNNHYL
                     GYNDWEVLEKIRQENHGADFHGDDPATIISRKTALMQNTFRTQPPILPGVVELLRLAK
                     LENIKLAVFSGALKQEIYTALNFLGIFSMFDSIMAAEDVAMGKPNPMGYLLSAERLGV
                     APKQCVVIEDSPIGLTAAKAAGMKTCALSTTYQPQTLSAANIVVPNLSCVTIENLKSL
                     FM"
     gene            complement(3486..4496)
                     /gene="tilS"
                     /locus_tag="PLK08_05825"
     CDS             complement(3486..4496)
                     /gene="tilS"
                     /locus_tag="PLK08_05825"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPS53052.1"
                     /translation="MRQSEPFLNELALALDLGLLAPQRNVLVGLSGGADSVALFTGLA
                     FLADCDGRKYRITAAHLNHQLRHDAHEDEIFVRDFCEKLDIPCICESMQVAEAADDMG
                     QGIEHAARELRYQFFKTSAVACGAEAVALAHHADDNAETILFRIIRGTGLRGLGGISP
                     VRPLCSDPPIRIIRPLLGVSKDMIVSFLRKHHITWREDHTNLDTNYSRNYLRHELIPA
                     VVSRLNGDFRNALCRLASQARLADEYIDRQSGNLLKIATILSETDRLLIDLDIFSQAD
                     EILQTTALRHATEILNIGQRDLTAEHIDDMKNLQPGQSVNLPQNFHAVVRRNMLVIEK
                     IL"
     gene            complement(4524..>5415)
                     /locus_tag="PLK08_05830"
     CDS             complement(4524..>5415)
                     /locus_tag="PLK08_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPS53053.1"
                     /translation="DIENELRRNFPKVCLVPRICDIYDRERVMSVWDEIRPNVVIHAA
                     AYKHVPMMEYNPCEAVKTNIFGSKNVADASVKYDVAEFITISTDKAVNPSSIMGCSKR
                     VVEIYTQALNWRENCKTKFKAVRFGNVLGSAGSVVPTFRKQIATGGPVTVTHPDMTRY
                     FMTIPEAAQLVLEAAATGQGGQIFLLDMGEPVKIADLAKQMITLSGFRPGEDIDIVYT
                     GIRPGEKLFEELRTQGEDIEPTVHPKIMIWTHSPIEWEKVQQIMDSLEPLKNTNDRDA
                     VVDALKKAVPEFVPLNPPKK"
BASE COUNT         1522 a         1263 c         1291 g         1339 t
ORIGIN      
        1 accaggttta cggcagaacg cctgacgaat tatcgcagaa catagaggat tttctttcga
       61 ttgtcgttcc ggaagataaa tcgcgggtaa gcaatgtcat tcaggatgca aaaaacggca
      121 ggactttttc gattgaatat tcgttgttca tgcccgacgg catggtgcgg actgtttttg
      181 aacgcggcga acccgaatac gacgaagccg gccagctaat cgcaataaac ggaacggttt
      241 tcgacgtaac agaacgggta ctagcggaac aggctatccg cagtagcgaa gaaaaagccc
      301 gcgttctcat gaattccacg cctgactttt ttgtgctgtg caacgctgac ggcataatcg
      361 ttgattgcaa cagtgcattt gtaaaatttc gcggcgtggc aagaagtaag cttatcggcc
      421 aatatctctt tgatttttac ataggcaagg acagggaaca gaaacgcaga cttttcatga
      481 aagtagtaga cacttttcag ccggtacatt tcgaagacgc aatgggcaac gtgtttctga
      541 gcactattct gtcgccgata aaagatgacg ccggccatgt ttcaatggtg ggcatttact
      601 cacgaaatct caccgagtac aaaaaaatcc aaaacgcgct taaagacagc gaagaacaac
      661 tcagggtaac gctggcgtca ataggcgacg gcgtcatcgc cacggatgcc aattccaacg
      721 ttatactcat gaattcggta gccgaaaatc ttaccggctg gaaattcgat gacgcactgg
      781 gcaaacctct cacggcagta ttccagattt taagcggcca caccagactg ccgattgtta
      841 accctgtggg cgacgttttg gcaacaggcc gaataagaca actcgccaac ggcattctgc
      901 tgatttcacg cgacggcaga gaatatcaga tatctgacag cgctgcacca atacgagatg
      961 aaaacggtaa tataagaggc gtagtgttga atttccgtaa catgacgctg gaataccagc
     1021 gcaggaaaat attgcaacga acgcagttct caattgacca tgcgcctctc gaaatatatc
     1081 tgctggacaa agacggcaga tacgtttacg cgaacaatct ggcaaaggcc aattttggcc
     1141 ttaccgacac atccattacc ggcacgacca tattcgatat taatccaggt atcgaccggc
     1201 aatggtggga aaaactgaag cgcgatgtaa tgttacaggg agtggtacat tgcgaaagta
     1261 cccatagaac ggcggacggc catacatatc cggtcgatgt ccggtattac agggtaaatc
     1321 tcgacgatgt ggattacctg tgcattttcg ccattgatat atccagcaaa aaacaggcgg
     1381 aagaacagcg gcagatgata ctggaagccg ccggcgacgg cattttcggc gtcgacgcat
     1441 tcggaaacat aatattcatg aatccggcgg catataaact gttcggttat gaagatgatg
     1501 aactcgtagg ccggccactg aaggaaatat tatgttcaaa tgactccgaa gacagtgcat
     1561 acgtaatgga gctttgcccg atggtgctgg cgtatacatg cggcattgaa agtcatatcg
     1621 acgacgaaat gctcccacga aaagatggca gcagatttcc cgctgaatat atcgccagac
     1681 cgcttaaacg gaaaaacgag ataataggcg cagtagtcac tctgcgggat atgaccgaaa
     1741 agaaaaaaac ggaatccgcg ctgcgagcca gtgaagaaca gtatcgctca ctattcgaat
     1801 cctcacgtga tgccgtgata ataatggacg aggccggcac atttttgaac gcgaatgatt
     1861 cggcggtaaa attgttcggt tatagaacca tctccgaact tcagggactt aaccctatgg
     1921 atacgtcgcc gaaaacacaa ccggaaggcc ttgcctctga aacattggct aaaatcagat
     1981 tcaaacaggc cctgaatgaa ggcggaagtt ttttcgagtg gcaacacaaa cgaaaggacg
     2041 gcagtgaatt tttgggtgag gtgtcattga atcccgtcat gtatagaggc catcgcgcca
     2101 tgcaggctgt gctgcgggat atcactgccc gcaaagaaat ggagcaaaag ctgcggcagt
     2161 ctctctcgga aatcgaaagc atttttgaaa atagcgccgt cggaatttta tatctctcgg
     2221 gcacagaacg aaaaatacag cgagccaata aacgatttct ggaattattg ggttacgaag
     2281 aaaatgaagt cctcggccaa agcgtaatca tgtttcatgt ttcaaaggca aaattcaagg
     2341 aatttgggaa atactataaa gaaaatttca tcaaaggcga aatggtcagg acggaaattc
     2401 cgcttaaacg aaaagatgga actgttattc aatgcatcat cagcggcaaa gctatcgatc
     2461 cgccagacct gagcaagggc gtcatatgga ttgtcgacct gtcatcactt atagaacagg
     2521 gtaattgcaa gtgacggttt attccatata tgcggcctta agacaccaga acctatccca
     2581 aaaactttta cttgcatgtg tacgtaattt cggttaaaca tctgccgcaa tgaaagcgat
     2641 attatttgat tttgacggcg taatagttga ttccgagccg ctgcattttg aaagcttttg
     2701 tgcagtgcta aagccctacg gaatcaacct cgaccgtgat tattataaca accattatct
     2761 tggttataat gactgggaag tgttggaaaa aatcaggcaa gaaaatcacg gcgcagattt
     2821 tcatggtgat gacccggcca caataatatc cagaaaaacg gcactgatgc aaaatacatt
     2881 cagaactcag ccgcctattt tgcctggagt agttgagttg ctccgactgg caaaactgga
     2941 aaatatcaaa ttggcggttt tcagtggggc gttgaaacag gaaatttata cggcgttgaa
     3001 ttttttgggt atttttagca tgtttgactc gattatggcg gctgaagatg tggcgatggg
     3061 caaaccgaac cccatgggct acctgctaag tgcggaacgc ctgggggtag ccccgaaaca
     3121 atgcgttgta atagaggatt cgcccatagg cctcacggca gccaaagccg ccggcatgaa
     3181 aacctgcgct ctttcaacaa catatcaacc ccaaaccctt tccgccgcca atattgtcgt
     3241 gccgaatctt tcctgcgtca cgattgaaaa tttaaaatca ctcttcatgt agaacccatc
     3301 tcaaaaactt tggctacctg caatcggccc tttcgtagtc tatagtaatt ctcgaaagtt
     3361 tccgtgaata gtctgtcgtt tctccttgta tggttaatgc cgtaatatgc agtaaagaca
     3421 gcttaaaacg atgattttgt ctttgcagat gttgccgaaa cattcacggt tattttcaag
     3481 aaccgctata aaatcttttc tatcacgagc atatttctgc gaacgacggc atgaaaattt
     3541 tgggggaggt ttacggattg gccgggttga agatttttca tgtcatcgat atgttccgcc
     3601 gtcaaatcac gctgacctat attcagaatt tcagtagcat gccgcaaggc ggttgtttgc
     3661 agaatttcgt ctgcttggct gaaaatatcc aggtcaatca gcagcctgtc tgtctctgac
     3721 aggatagtgg ctattttaag aagatttcca gactgtctgt cgatatattc atcagccaac
     3781 cgagcctggc ttgcaaggcg acaaagcgca tttcgaaaat ctccgttcag ccgcgacaca
     3841 acagcaggta taagttcatg tcgcaaataa tttcgagaat aattagtgtc caggttcgta
     3901 tggtcttccc gccacgtaat atgatgtttg cgaagaaagg agactatcat atcttttgag
     3961 acacccaaca acggccttat tatacgaata ggcgggtcgc tgcataacgg ccgcaccgga
     4021 gagattccgc caagcccgcg caagccagtg ccgcggatta ttctgaaaag aatggtttct
     4081 gcattgtcgt cggcatgatg cgccaaggca acggcctccg cgccgcatgc aaccgccgac
     4141 gttttgaaaa attgataacg aagctccctg gcggcatgtt cgattccctg gcccatatcg
     4201 tcggccgcct cagccacctg cattgattcg catatgcatg gaatatcgag tttttcgcaa
     4261 aaatcacgga cgaaaatttc atcctcatgt gcatcatgcc gtaattgatg attgagatgc
     4321 gcagcggtta tccgatattt cctgccgtca caatcggcaa gaaacgccag gcccgtgaac
     4381 aaggcaacgc tgtcagcacc tcccgaaagt ccgacaagta cattccgctg cggcgcaaga
     4441 agacctaaat ccagggccaa cgccagttcg ttcaggaaag gctcggattg tcgcatttgg
     4501 gaaaaaattg ctcaccccca aaattatttt ttaggcggat taagcgggac aaattccggc
     4561 acagctttct taagcgcgtc cactacggca tcacggtcat ttgtattttt aagcggctct
     4621 aaggaatcca ttatctgctg aaccttttcc cactcaatcg gcgaatgcgt ccatatcata
     4681 attttgggat gaacggtcgg ttcaatatcc tcaccctgtg tgcgaagttc ctcgaacagt
     4741 ttttcgccgg gccgtatgcc ggtataaacg atatcgatat cctcgccggg ccgaaaaccg
     4801 ctcagtgtta tcatctgttt ggcaaggtcg gcaattttta ccggctcacc catgtccaac
     4861 aaaaatatct ggccgccctg gccggttgcc gctgcttcca atacaagctg ggccgcttca
     4921 ggaatggtca tgaaataccg cgtcatgtcg ggatgcgtaa cagttacggg gccaccagtg
     4981 gctatctgct ttctgaacgt cggcactaca gagccggctg acccaagtac gttgccgaaa
     5041 cgaaccgcct tgaatttggt tttacaattc tcgcgccagt tcaacgcctg cgtatatatt
     5101 tcaacaacac gttttgaaca gcccatgatg gatgatggat tgacggcttt gtcggtggag
     5161 atggtgataa attccgcaac atcgtatttt acggaagcat cggctacgtt ttttgaaccg
     5221 aaaatattgg ttttgacggc ttcacatgga ttgtattcca tcatcggaac atgcttgtag
     5281 gcggcggcgt gaattaccac attcggccgt atttcgtccc agacggacat cacacgctca
     5341 cggtcatata tgtcgcatat tctaggcacg aggcatactt tgggaaaatt ccgacgcagt
     5401 tcattttcga tgtcg
//
LOCUS       DAONFI010000146         5413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17806_length_5413_cov_3.641844, whole genome shotgun sequence.
ACCESSION   DAONFI010000146 DAONFI010000000
VERSION     DAONFI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17806_length_5413_cov_3.641844"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLK08_05835"
     CDS             <1..326
                     /locus_tag="PLK08_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53054.1"
                     /translation="QRLRQWYKPAKGEEFAAYAPDRSKNRTEDGMSGLVVTNRRLIYN
                     CSLRQKETGKTRPTDMDFTMENGRLKLDIKTPDWEVKGLFVDKAGLERLRRAMIAEQF
                     NATWR"
     regulatory      503..617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            778..2070
                     /locus_tag="PLK08_05840"
     CDS             778..2070
                     /locus_tag="PLK08_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS53055.1"
                     /translation="MAKDNSYKLGTKAIHGGYETEPTTMSCGVPLYLTSSYLFRDTSH
                     AARLFELSEPGNIYSRLQNPTCQILERRLAELDGGSGAVTLASGMAAISAAVFTLTKQ
                     GDNIVSSQCLYGGTVTLFGQTLPRYGVNVRFFDPENVDTVEPLIDSKTRAVYMETIGN
                     PINVVPDFERIAEIAHRHNVPVIVDNTVATACLFKPLEHGADIVVYSTTKFIGGHGTH
                     LGGAVVDGGFNWAADRRKWADFTAPDAAYHGIVFSDLAGPATFVEYIRTHWLRDTGGA
                     MSPFAAWIFLQCLETLHLRVPQHSRNAQAVAEFLNSHPFVDLVNYPGLPGHGSYERAQ
                     KYLPNGKGGIIGFGIKGGRPAGEKFINAVKLASHLANIGDAKTLVIHPASTTHQQLSE
                     DELRAAGVKPEFIRLSVGIEDIDDIIADLRQALEKSQK"
     gene            2089..3897
                     /locus_tag="PLK08_05845"
     CDS             2089..3897
                     /locus_tag="PLK08_05845"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPS53056.1"
                     /translation="MSDIFSGSDEARNGGTLKYVKTVTFPGEFRLERGQTLTDVKAAY
                     ETYGKLNAGRDNAILICHALTGDSHVASHDENDDSGWWEILVGPDKPIDTNRYFVICP
                     NVLGSCRGSTGPNSIDPNTGREYAGDFPAITIGDMVELHRRVIDSLGIEKLLAVIGGS
                     MGGHQTLEWARRYPERTGGAILMATSPRLSSQSLSFDIIGRNAIRQDPNFCDGNFYDK
                     PKGPAVGLALARMLGHITYLSPAAMNEKFEATRNQPRDVDTIFEKEFSVGSYLAYKGD
                     KFTGRFDANSYIVLSMAMDQFDLGATVEQLRKNLEAAKNRWLVVSFTGDWLFPAEQAK
                     QIVHSLVLSRKPVSYCNITSECGHDAFLLEEDFDKYGRLVDGFLRSLSGEKIRDDGIE
                     PIDYSPTSIFHRHRRLDYDRIIDFINPEDSVLDIGCGHGGLLERLQKQGNKRVAGVEI
                     SADSIIECVRWGLNVIQEDINNGLGWFSDSQFDVVVLSRTIQAVYDVEHVMDEMLRVG
                     KRCIVSVPNFGYYKLRDMLARQGRAPEAGLLHYKWYNTPNIRVLTLRDFEEFCVAKNI
                     TIHRMVAIETETDRELPATEEHYELADIAIYEICRK"
     gene            3997..5253
                     /locus_tag="PLK08_05850"
     CDS             3997..5253
                     /locus_tag="PLK08_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPS53057.1"
                     /translation="MKAVVFEVKPAGWMLCRLFAKKFPALRKSALDGFELREIPVPQL
                     PGDDWVLLRTRLGGICGSDVALFTQKQPPNSLLQTYTSQPMLIGHENVATVEKVGSAV
                     DSSWLGRRVTAEPTLGCQPRGTVPMCPRCQAGEFGACENFSGDMGGRYSLPAGSSIGY
                     NSATGGSWGEFFVAHKSQLIALDDETISDEEALLIDPFACALHAVLRANSSAASRVLI
                     YGSGVLAMGTVASLRATGFKGKIEVLCGRSKQLQEAVARMGADECFVLPRKKSERFEI
                     MARRIGGTLQRARLGNYMISGGYDVVFDCIGTAVSFMECLKFARARGQVVMLGTLQEQ
                     ILDISPLWFRELCILGSWGRSVENFDGQRLTTYELVLKFLKERKISTAGLVTNFFRIE
                     DYRAALLASADKFSSGSIKTAFDFRN"
     gene            5291..>5413
                     /locus_tag="PLK08_05855"
     CDS             5291..>5413
                     /locus_tag="PLK08_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HPS53058.1"
                     /translation="MENPVVGVVMGSDSDFETMKACTAMLDFFGISHEVRIISAH"
BASE COUNT         1321 a         1436 c         1511 g         1145 t
ORIGIN      
        1 agcagcgtct gcgacagtgg tacaagccgg caaaaggcga ggaattcgcc gcctatgcgc
       61 cagaccgttc aaaaaaccgt acggaagacg gcatgagcgg cctggtggtg acaaataggc
      121 ggctgattta caactgctcg ctgcggcaga aggaaaccgg caaaacacgg cctacggata
      181 tggacttcac aatggagaat ggccggctga agttggacat caaaacgccg gactgggaag
      241 taaaaggtct ttttgtcgac aaggctggac tcgaacgtct caggcgggca atgatagcag
      301 agcagttcaa cgccacgtgg cgataaatag agcattttaa agttgcgact tccttttttg
      361 ctggtttgct ttgttcgtgg ctgcaaacag cagcaaacgc aagccaggtt ttatatgctc
      421 taattccatt atgctaaaca aaaaattgat attttaaaca aagtttactt gacacggcag
      481 acggctgagg atagtattcg gcttggcatc gagagtgcgc gagggtactg gccctgagac
      541 cgcacggcaa cctgtcgaac atatttattt tatgcagata aggtgctatc agccagccct
      601 aaagggagcg atgcggccgg taagccgtta ttagccgaca actcgcaaag caatatattt
      661 cgccgccatg cgaccatgac cggcaagtcc gttgcacccc ttcaggcttt tttgggaaag
      721 ttttttatgc gccttattta cgcgcgacgg cgaaggacca gaaaggattt ttagaaaatg
      781 gctaaggaca acagttataa actcggcacg aaggcgatac acggcggtta cgagactgaa
      841 ccgacgacca tgagttgcgg cgtgccgtta tatcttacca gcagttacct tttccgcgac
      901 accagccacg ccgcgcggct gttcgaattg agcgagcccg gcaacattta ttcgcgtctg
      961 cagaatccca cctgccagat acttgagcgt cgtcttgccg agcttgacgg cggcagcggc
     1021 gcagtgacgc tagccagcgg catggctgcg atatcggcgg cggttttcac gctcactaaa
     1081 cagggcgaca atatagtatc gtcgcaatgc ctctacggcg gcacggtcac gctcttcggc
     1141 cagacgctgc cgcgatacgg cgtaaacgtg cgattcttcg accctgaaaa cgtcgatacc
     1201 gtcgagccgc tcatcgactc caaaacccgc gcggtttata tggaaaccat cggcaacccg
     1261 attaacgtcg tgccggattt cgagcgtatc gcggaaatcg cccatcgcca caatgtgccg
     1321 gtaatcgtcg acaataccgt cgcgaccgcc tgccttttca agccgctcga acacggcgcc
     1381 gacatagtgg tttattcgac caccaagttc atcggcggcc acggtacgca tctcggcgga
     1441 gcggtcgtgg acggcggttt caactgggcg gctgaccgca ggaaatgggc cgatttcacc
     1501 gcaccggacg cggcatatca cggcatcgta ttcagcgacc tggccgggcc ggcgactttc
     1561 gtggaatata tccgcacaca ctggctgcgc gacacgggcg gcgcgatgag tccgtttgcg
     1621 gcatggattt tcctgcaatg ccttgaaact ctccacctgc gagtgccgca gcattcccgc
     1681 aatgcgcagg ctgtggcgga atttctgaat tcgcatccgt tcgtggactt ggtcaactac
     1741 cctggcctgc cgggccacgg ctcatatgaa agagcccaaa aatatctgcc gaacggcaag
     1801 ggcggcatta tcggcttcgg cataaagggc ggccggccag ccggcgaaaa attcatcaac
     1861 gccgtaaaac tcgccagcca tctggctaac atcggcgatg caaaaacgct tgtcatccac
     1921 cctgccagca caacgcacca gcagctaagc gaagacgaac tgcgggcagc cggcgttaaa
     1981 ccggaattca tacggctgtc ggtcggcatt gaagacatcg acgacatcat cgccgacctc
     2041 aggcaggcat tggaaaaatc acaaaagtag ttggaaggta tttgggaaat gagcgacatt
     2101 ttttccggca gtgatgaagc ccgcaacggc gggacattga aatacgtcaa aacagtgacg
     2161 tttccaggcg agttccgtct tgagcgcggc cagactctca ccgacgtaaa ggctgcgtat
     2221 gagacatacg gcaaactcaa cgccgggcgc gacaacgcca tattgatatg tcatgcgctg
     2281 accggcgatt cacacgtcgc cagccatgac gaaaacgacg attccggctg gtgggaaatt
     2341 cttgtaggcc ctgacaaacc tatcgacacg aacaggtatt ttgtaatatg cccaaacgtt
     2401 ctgggcagtt gtcgcgggtc aaccggtccg aacagcatag acccgaatac cggccgtgaa
     2461 tatgcaggcg attttccggc gataaccatc ggcgacatgg ttgaacttca tcgccgcgtc
     2521 atagatagcc ttggcataga gaaactctta gccgtaatcg gcggctcgat gggcggccat
     2581 cagacgctgg aatgggcgag acgttatcct gagcgtaccg gcggagcgat attgatggct
     2641 acgtcgccca ggctgtccag ccaatcgctt tcgttcgaca tcatcggcag aaacgccata
     2701 cggcaggacc cgaatttctg cgacggcaat ttttacgaca agcccaaagg cccagccgtc
     2761 ggccttgcgc tggcgagaat gctgggccat ataacctatc tttcgcctgc cgcgatgaac
     2821 gaaaaattcg aagccacccg caatcagccc cgcgacgtgg atacgatttt cgaaaaggag
     2881 ttctccgtcg ggtcttacct tgcctacaag ggcgataaat tcactggccg tttcgatgca
     2941 aacagctata tagttctgtc gatggccatg gatcagttcg acctcggcgc gacggtcgaa
     3001 cagttgcgta aaaatctgga agccgccaaa aaccgctggc tggtggtgag tttcactggc
     3061 gactggctgt ttcccgctga acaggccaaa cagatagtcc attcgctggt tctgtccaga
     3121 aagcccgtga gctactgcaa tatcaccagc gagtgcggcc acgatgcgtt tctgctggaa
     3181 gaggattttg acaaatacgg ccgcctggtc gacggtttcc tgcgaagcct ttcaggcgaa
     3241 aaaatcaggg acgacggcat tgaacctatc gattacagcc cgacgagcat atttcacaga
     3301 catcgccggc tggattacga ccgcataatt gacttcatca atccggaaga ttctgtgctt
     3361 gacataggct gcggccacgg cggcttgctg gaacgactgc aaaaacaggg caacaaacgg
     3421 gtcgccggcg ttgagatttc cgccgacagc ataatagaat gcgtccgttg gggtcttaac
     3481 gtcattcagg aagacatcaa caacggcttg ggctggttca gcgacagcca gttcgacgtg
     3541 gtcgtgctct cccgaacgat acaggcggtc tacgacgtcg aacacgtaat ggatgaaatg
     3601 ctgcgggtcg gcaaacgctg tatagtatcc gtgccgaatt tcggttatta taagctccgc
     3661 gacatgttgg ccaggcaggg ccgggctccg gaagccggcc ttctgcacta caaatggtat
     3721 aatacgccca acatccgcgt gctgacgctg cgggactttg aagagttctg cgtcgccaaa
     3781 aacataacga tacatcgcat ggttgcgata gagacggaga cagaccgcga actgcccgct
     3841 accgaggaac actacgaatt agccgatatc gcaatatatg aaatctgccg caaatagcgt
     3901 gcacgacgtg aactgacgat gatttttgca tggaaacggt tgaaaaccgt agaattgttt
     3961 gactgaagaa atccagaata aggactccct tgtcatatga aggccgtcgt atttgaagta
     4021 aaacctgccg ggtggatgtt atgcaggctg tttgccaaaa aatttccggc gcttcgtaaa
     4081 agcgcgttgg acggttttga attgcgggaa attcccgtgc cgcagcttcc cggcgacgac
     4141 tgggtactgc ttcgcaccag gctgggcggc atatgcggtt cagacgttgc gttatttacc
     4201 cagaagcagc cgccgaattc gctgttgcag acatacacgt cccagcccat gctcataggc
     4261 catgaaaacg tggcgacggt ggaaaaagtc ggctctgcag tcgattcatc atggctgggc
     4321 cggcgtgtga cggctgaacc cacgctcggc tgtcagccgc gcggcactgt gccgatgtgc
     4381 ccgcgctgcc aggcaggcga gttcggggca tgtgaaaatt tttccggcga catgggcggc
     4441 agatattcac tgccggccgg ctcaagcata gggtacaatt ccgccaccgg cggctcatgg
     4501 ggcgaattct ttgtggcaca caagtcgcaa ctcatagccc ttgacgatga aacgatatcc
     4561 gacgaagagg ctttgctaat cgacccgttc gcatgcgccc ttcacgccgt attgcgagca
     4621 aactcgtctg cggcaagccg tgtgctcata tacggcagcg gcgtattggc gatggggacg
     4681 gtggcgtcat tgcgtgcgac cggttttaag ggaaaaatcg aagtgctctg cggccgctca
     4741 aaacagctgc aggaagcagt cgcccgcatg ggtgcggatg aatgcttcgt tctgccgcgg
     4801 aaaaaatcgg agcgattcga aataatggcc cgtcgtatcg gcggcacgct tcagagggcg
     4861 cggctgggca attacatgat aagcggcggt tatgacgtgg tttttgactg catcggcaca
     4921 gccgtaagtt ttatggaatg tctcaaattc gcccgcgcac gcggccaggt agtcatgctc
     4981 ggcacgctcc aggaacagat actcgacatc tcgccgctgt ggtttcgcga attgtgcatt
     5041 ctgggcagtt ggggcaggtc tgtggaaaat tttgatggcc aacggttgac aacatatgag
     5101 cttgtgctaa agtttctcaa ggaacggaaa atttcgaccg ccggcctggt gacgaatttt
     5161 ttcagaatag aagattatcg ggccgccctg ctggcgtcag ccgacaaatt ttccagcggc
     5221 tcaataaaaa ccgcgttcga tttcagaaat tgacaaataa cggacatatt cacgcaagga
     5281 gacgactgat atggaaaacc ccgtagtcgg agtcgtaatg ggcagcgaca gcgattttga
     5341 gaccatgaag gcctgcactg ccatgctgga ttttttcggc atatcacacg aagtgcggat
     5401 aatttccgct cat
//
LOCUS       DAONFI010000147         5400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17852_length_5400_cov_3.481197, whole genome shotgun sequence.
ACCESSION   DAONFI010000147 DAONFI010000000
VERSION     DAONFI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17852_length_5400_cov_3.481197"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PLK08_05860"
     CDS             <1..1486
                     /locus_tag="PLK08_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53059.1"
                     /translation="ESVDGINWTRPKLGKNANNIMAGLTDPKIYNNRNSDNPAKKYIV
                     PLHTSMSPEYRKPENARMSYTSNHPSIKCFAYSINGVDWKIDDDASKLFPLGMKFEAP
                     VLYKMHDTWFLIGQCLQGEYPSVQKNSRYLVVFSSKDMKNWVMSDCPGFSFPQKYNGM
                     LQTHITPGIITYPNISIGIEGMFQDSPELMDQETDLIVLITNDGYRWRLPKAQGYPYA
                     ILRRGDLGSWDQSFLLQSAPINVGDTTRIYYGGMAHGNASYIGGRIGFAYIGLDRYGY
                     MGLKTGWSFMKDVEFIGKIISKPISLGDGHSLKLYVNQEVPEKIKEIPAIPSVINVAL
                     LDADGKPLSGYTSEDCDSLDVSGVGVPVTWKGNSDISSLAGKKIRVQYQMRAKSNKNP
                     LFRSFTPKFYGFYFDNPTLWENVPTNIYGKRGLERLAFSDSAHKFLKDIEITSDVPMK
                     VTLSQIKSDGAYLRFDGNGKAWIKYRNKITEVDVAGQKEVILGK"
     gene            complement(1862..1935)
                     /locus_tag="PLK08_05865"
     tRNA            complement(1862..1935)
                     /locus_tag="PLK08_05865"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1899..1901),aa:Val,seq:cac)
     gene            complement(2013..3179)
                     /locus_tag="PLK08_05870"
     CDS             complement(2013..3179)
                     /locus_tag="PLK08_05870"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPS53060.1"
                     /translation="MQNLDTEADFPEDFDGTDGDEPQSVEISEDGLDAVETSFDAEED
                     GLRHVRVEIRRLKENKRIDKYLSHRLGKGVSRTALQKYIAEGIVKVNGRTVKCSYNLR
                     VGDAVDMMLPETRPKEIPAEPIPLDVVYEDEDMMAINKQANLIIHPARGNWSGTLVNA
                     LAYYFQANWRTGQSINDLPCNGEKFRPGIVHRLDRDTTGIILIAKTDLALWRLGRQFE
                     DRSTSKTYSAIVHGLLDFDEDIIDMPIGKHPKYREKYAVHRDTGKPHPITVKSAVTRY
                     KVVERLQPKGTDMGFTLVELHPKTGRTHQLRVHMSAIGHPMVGDRMYGGGPLYISQLD
                     GHAEVAEGPLITRQALHAHTIEFYHPRTNERMSLTAPWPKDFEDTLAELRRRCK"
     gene            complement(3179..3850)
                     /locus_tag="PLK08_05875"
     CDS             complement(3179..3850)
                     /locus_tag="PLK08_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53061.1"
                     /translation="MDSDRRHELEHNDLDTELSKLIAFVKNNINRILTVVIVILAIAV
                     AYTYISREMKSRRASIQSRYDSVIARTAMPGAKTEELLAEFNELSDQSSVDWIAANSL
                     LQIGRLHSKQASESADESKRKQSFEKARQTYNQAVDKYDDQPRVVASAIFGLGKIAED
                     MGNYDEAKADYTRIANDKSLAGYPIQKQAEEALKRIAQFKGRPQLATTRPAWMQDKTP
                     APKKP"
     gene            complement(3875..4318)
                     /locus_tag="PLK08_05880"
     CDS             complement(3875..4318)
                     /locus_tag="PLK08_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53062.1"
                     /translation="MVTKIAKLLCVMAAVAMLAGGCANQNAGLVGMPSPILQDIPIPT
                     DFKIVNDRSRSWTDGTLRFADLLYKGRADSTAIQKFYSQQMPTCHWIAQTEMFAQGRS
                     TMDYVKSGEKCRVTVYKEGVFGETYIQVAIWPNRPNAKIDPNQNR"
     gene            4565..4894
                     /locus_tag="PLK08_05885"
     CDS             4565..4894
                     /locus_tag="PLK08_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HPS53063.1"
                     /translation="MIAVLNLFDIIPGKEKEYAKYLRLVQPVLDRHNAQVMIYGTTRM
                     TFMGDDPQEYCGIIGYPSMRHLRAFSTDPEFETIRSLRDNSTMNYVLSVIEHVGTLAA
                     AADDLER"
     gene            5081..>5400
                     /locus_tag="PLK08_05890"
     CDS             5081..>5400
                     /locus_tag="PLK08_05890"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPS53064.1"
                     /translation="MISDSVKKGFERSPHRSLMKAAGLADSDIRKPFIAIANSYTDIV
                     PGHCHLDAVAEYVKAQVELAGGTAFIFNTPAVCDGIAMGHTGMKYSLASREIIADSVE
                     TMVRA"
BASE COUNT         1354 a         1221 c         1344 g         1481 t
ORIGIN      
        1 ggaaagcgtc gatggaatta actggactcg accgaaattg ggaaaaaacg ccaataatat
       61 tatggctggc ttgaccgatc ctaaaatata taacaaccgt aacagtgata atccggcaaa
      121 aaaatatatt gttccattgc atacgtcaat gtctcccgaa taccgtaagc cggaaaatgc
      181 caggatgagt tatacctcca accatccatc aataaaatgc tttgcgtaca gcatcaacgg
      241 cgttgactgg aaaatagacg acgatgcctc caaacttttt ccattgggca tgaaatttga
      301 agctcccgtc ctttataaaa tgcatgatac gtggtttctt atcggccagt gcttacaggg
      361 agagtatcct tccgttcaaa aaaactcgcg ttatcttgtg gtattttcct cgaaggacat
      421 gaaaaattgg gtaatgtcgg actgccccgg attttcattt cctcaaaaat ataacggcat
      481 gcttcaaacg catattacgc ccgggattat tacatatccc aatatttcca tcggaattga
      541 aggcatgttt caggattcgc ctgagctgat ggatcaggag acggacctta tagtgctgat
      601 aaccaatgac ggctatcgct ggcgattgcc caaggctcag ggctatccgt atgcaatatt
      661 gcgacgtggc gatttgggga gttgggatca gagctttctg cttcaaagtg ctccgattaa
      721 cgttggcgat accacgcgaa tatattatgg cggtatggct catggcaatg cttcatatat
      781 tggcggcaga atcggattcg catatatcgg cctggatcgt tacggttaca tggggctgaa
      841 gacgggctgg agttttatga aggacgttga attcatcggc aagataattt caaaaccgat
      901 ttcgcttggc gatggtcata gcctcaagct gtacgtcaac caggaagttc ctgaaaaaat
      961 caaagaaata cctgcaattc cttcagtgat taatgtggcg ttacttgatg ctgacggcaa
     1021 gccgctgagt ggttatacgt cggaagattg tgattcgctt gacgtaagtg gcgttggagt
     1081 gcctgttaca tggaaaggta actcggacat ttcatcgttg gctggcaaga aaattcgcgt
     1141 tcaatatcag atgcgggcaa aaagtaataa aaacccgtta ttccggagtt ttacgccgaa
     1201 attttacggg ttttattttg ataatccgac attgtgggaa aacgtaccga caaatatcta
     1261 cggcaagcgt ggcttggaga gattggcgtt ttcagattcg gcacataagt ttttgaagga
     1321 tattgaaata acatctgatg ttccgatgaa ggtaacactg agtcaaatta aatctgacgg
     1381 ggcgtatctt aggtttgatg gtaatggcaa ggcttggatt aaatatcgga ataaaattac
     1441 ggaagtagat gtagcaggcc aaaaagaagt tatattgggt aagtgagaaa taaaaactga
     1501 aaagaaatga atatctcttt ttagtttttt agagaggcca tcctgatttt attcgtaatg
     1561 aaataaaacc gcatatcttt tgctgaattt gaacctggtg attcgaggct gctccagcca
     1621 tttgccatac tattcatcgt ttatgggcgt atgcgatgaa agtgtataaa atatcaaatt
     1681 ttctgaatta atgaccaccg gcagagccgg tggtttgaga ggcacggcag gtgcatgagc
     1741 ctgcatgcaa taagatgaaa gtcgaaaagc cggaaacgat tttagccaat atttacaggc
     1801 ctaattgcga gatattaccg ttgatttcac ctgctttcga tatgaaagca atagaaagca
     1861 ctgggcctta taggactcga acctatgacc tcacgggtgt gatccgtgcg ctctaaccaa
     1921 ctgagctaaa ggcccttcaa aaattccaga ttatataaac gctgcgatgt attgcaacct
     1981 cttttccaca cgaatatttt tataagcttt tattatttgc atcgacggcg aagctctgcc
     2041 agagtgtctt caaaatcttt cggccaggga gctgtaagag acatacgctc attcgtccgc
     2101 ggatgataaa attctatcgt atgtgcatgg agagcctgcc gcgtaatgag cggcccctca
     2161 gcaacttccg catggccgtc cagctgactg atataaagcg gcccgccgcc gtacatacga
     2221 tcgccaacca ttggatggcc tatcgcactc atgtgcaccc gaagctgatg cgttctgcca
     2281 gttttcggat gaagctccac cagcgtaaaa cccatgtccg tacctttcgg ctgtaaccgt
     2341 tccactacct tgtatcttgt cacggctgat ttgacggtaa tcgggtgcgg cttgccggtg
     2401 tcgcgatgaa cagcgtattt ttcgcgatat ttaggatgct tgccgatggg catgtcgatt
     2461 atatcttcgt cgaaatcaag aaggccatgc acgattgcgg aatatgtttt gctcgtcgaa
     2521 cggtcctcaa actgacggcc cagccgccat aacgcaaggt ccgtcttggc gatgagaata
     2581 atgccggtcg tgtcacggtc aagacgatgt actataccag gcctgaactt ttcgccattg
     2641 cacggcaaat cgttaatcga ttggccggtt cgccagtttg cctgaaaata ataggctaac
     2701 gcatttacca gtgtgccgct ccaattccca cgtgccggat gaatgatgag attagcctgt
     2761 ttgtttatcg ccatcatatc ttcatcttca tacacgacgt ccagcggaat tggttcggcg
     2821 ggtatttctt taggccgtgt ttccggcagc atcatatcaa cagcgtcgcc gactcgcagg
     2881 ttgtaactgc attttactgt tcggccgttg actttgacga taccttcggc gatgtatttc
     2941 tgcaaagcgg tacgcgatac tcccttgccg aggcggtgtg agagatattt gtcgatacgc
     3001 ttgttttctt tcaggcgtcg aatttccacg cgtacatgac gaaggccgtc ttcttcggcg
     3061 tcgaatgaag tttctacggc atcaaggccg tcttccgaaa tttctacaga ctgcggctca
     3121 tcgccgtctg tgccgtcgaa atcttcggga aagtccgctt cagtgtcgag gttctgcatc
     3181 aaggtttttt gggggccggt gttttatctt gcatccacgc cggtctggtt gtagccaact
     3241 gtggccgacc tttaaattga gcgatacgct taagcgcttc ttccgcctgc ttctgaatgg
     3301 gataacctgc aagggatttg tcgttggcta ttctggtgta atcggctttc gcttcgtcgt
     3361 aattgcccat gtcttcggct attttgccca agccgaaaat ggctgatgcc actactcgcg
     3421 gctggtcgtc atatttatcc actgcctgat tgtatgtctg ccgggccttt tcgaaggatt
     3481 gtttacgttt agactcgtcg gctgattctg aggcctgttt tgaatgaaga cggcctatct
     3541 gcagaagtga atttgcagca atccagtcta cggacgactg gtcggacagt tcgttgaatt
     3601 cggcaaggag ttcttcggtt tttgcaccag gcatggcggt gcgggcaatg acggagtcgt
     3661 agcggctctg aatagacgca cgacgcgact tcatttccct gcttatgtag gtataggcaa
     3721 cggcgatggc cagaattacg ataacgaccg ttaatatgcg attgatattg tttttgacaa
     3781 acgcaatcag tttcgaaagt tcagtatcaa ggtcgttgtg ttcaagttca tgacggcgat
     3841 ccgagtccat ttgtgctgca ttccttccgt tgaattatct gttctggttt gggtctattt
     3901 tcgcattcgg gcgattgggc cagatggcta cctgaatgta cgtttcaccg aagacgcctt
     3961 ctttgtagac ggtcacgcgg catttctctc cgcttttgac gtagtccatg gttgaacggc
     4021 cctgggcgaa catttcagtc tgggctatcc aatggcaggt gggcatctgc tgcgaataga
     4081 atttctggat ggcggtagag tcggctctgc ctttgtacag caggtcagcg aatcgtaatg
     4141 tgccgtctgt ccagctccgt gagcggtcgt tgactatctt gaaatcggtt gggataggta
     4201 tatcctgcaa tatcgggctt ggcatgccga caaggcctgc gttctggttg gcacagccgc
     4261 cggccagcat tgcgacagcc gccatgacgc ataacagttt tgcaattttc gtaaccatat
     4321 tgtctccttg tgcgtacagt agctaatttt cgcagaaaat ctcttcgcga gcaagaggaa
     4381 gatggaattt ttttgtcggg cttctttttt atagggcaaa ttagcccgta cttttaaaaa
     4441 ccgacaaaat attgaaagac gtatggaacg gtattatttg tggttgtaaa tcaggctgga
     4501 aaagcgtaac ctgtctgaac gtcgcctgcg ggacaggcgc ggctgaattt acaggagaac
     4561 ggcagtgata gctgttttga atttgtttga cataatacca ggcaaagaaa aagaatacgc
     4621 caaatatctt cggctggttc agcccgtatt ggatcgccac aatgcgcagg tgatgatata
     4681 cggaacgacc cgcatgactt tcatgggcga cgatccgcag gaatattgcg gcattatcgg
     4741 atatcccagc atgcggcatc tgcgggcgtt ttccacagac cccgaatttg aaaccatccg
     4801 ctcgttacgc gacaattcga cgatgaatta cgtgttatcc gtaatcgaac acgtcggcac
     4861 gctggccgct gccgctgacg acctggaaag atgacctttc gcggatgtgc cgttgaatct
     4921 gataaaagtg aatttctacc ggtttagaac cagtagcgtc attattttca ggctttcgtc
     4981 tgtctgacga cggctgccgt catctacggt ttttaaaaaa accgtgaaat tgcgaagaaa
     5041 atattgaaaa atcgacggat atctacaagg agtaagaatc atgataagcg atagtgtcaa
     5101 gaaaggtttt gaacgttcac cgcacaggtc actgatgaag gctgccggcc tggccgacag
     5161 cgacatccgt aagccgttca ttgccattgc caacagttat accgacatcg ttcccggcca
     5221 ctgtcatctc gacgccgtgg ctgaatacgt caaggctcag gtcgaacttg ccggcggcac
     5281 ggcattcatt ttcaacacgc cagccgtctg cgacggcatc gccatgggcc acaccggcat
     5341 gaaatattcg ctcgccagcc gtgaaatcat cgccgacagc gttgaaacca tggttcgcgc
//
LOCUS       DAONFI010000148         5399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17854_length_5399_cov_3.904753, whole genome shotgun sequence.
ACCESSION   DAONFI010000148 DAONFI010000000
VERSION     DAONFI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17854_length_5399_cov_3.904753"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /gene="secF"
                     /locus_tag="PLK08_05895"
     CDS             <1..1162
                     /gene="secF"
                     /locus_tag="PLK08_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPS53065.1"
                     /translation="PQRTYQISTTSADVPLIRKVLNEAFGEAVERRVPCTYNMVKNTN
                     IPQLGVKTADGGATIVTPALIKQASASVRDELMDFEGGVMFVIDDVKPAITVADLTGR
                     INDIRSQPDFEKQAGNPTRVIGLKGNGEDFNSFAVIVRPADAEIMSKPVLREQFLDGE
                     RTVLASALEREDPTIITSFDASIAKDTASYAIVAVILSWLTIIVYLWVRFGSAQWGLA
                     AVLCLVHDVLIVVGMVAVSGWLAGTRVGNALGVGVFKIDLTMIAAILTIIGYSVNDTI
                     VVFDRIRENRGKLKAVTADCINDSINQTLPRTLLTSFTTFLVVLVMYIFGGDGMRSFN
                     YGLLIGIVVGTYSSIAIASPLLLGFKYAIIARTDEDDTSEQNDDTQAVLPQE"
     gene            1300..2790
                     /gene="gatB"
                     /locus_tag="PLK08_05900"
     CDS             1300..2790
                     /gene="gatB"
                     /locus_tag="PLK08_05900"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPS53066.1"
                     /translation="MSKEAAKPDISVLPVIGLEIHVELATKTKMFCRCRNNFGDAPNT
                     NVCPVCTSMPGALPVMNRQAVEYAMKVGLALGCTVPSYTKWDRKNYYYPDLPKGYQLS
                     QYDLPLCKDGVVEYPLDGGGVKPLRVLRAHLEEDAGKNMHDIPGATGVDLNRAGVPLL
                     EIVSQPDMNTVEEVMYYARGMHRLVRWLGVSEANMQMGHMRFEPNINLHITWKGKVYK
                     TPIVEVKNLNSFRSLEGAVKYEIRRQYNEWKNDPDGFSLKKLGKQNRGYDVENEQTVF
                     QRDKEEAHDYRYFPDPDLPAVIVGDEWRNAVLSQIGELPFARRSRYIDELGITFQEAD
                     ALTQESSTGELFDAAVKSGADAKRCCNLLLGRGAAIANERGCSIAEIGITAEQLGELA
                     IMLENGDVNATAAATIFDKMLESSCKSPRLIAETDGLLIVRDVAQLNAWVDAAIAANP
                     QAVEEIRSGGKKKEKSFGFLTGQVMQASRGAANAKEVQSLLKEKLS"
     gene            2816..3760
                     /gene="gluQRS"
                     /locus_tag="PLK08_05905"
     CDS             2816..3760
                     /gene="gluQRS"
                     /locus_tag="PLK08_05905"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HPS53067.1"
                     /translation="MTRQFTTRLAPSPTGALHLGNARTFLANWLLAKQRSWRVLFRME
                     DLDTPRTKSWAADAAITELKWLGLDWGGDVITQSQRSEAYEAALKNLADRGSAYPCNC
                     SRRDVETSASAPAAEDRLGQQAAVYPGTCRNKNIDPAGAAWRVKVDDAVIEFDDNFAG
                     PQSFNLSQTSGDFVIFRKTHQAAYQLAVVVDDAESGVDAIVRGDDLLESAAMQMYLRR
                     LLGYDERMEYWHLPLVLGPDSRKLAKRHGDTRLCHYVSLGVTRQRMLGLLGYWLGTLE
                     NRRETDISELAEKFDINKISAKPVIFGRDDHDFLVGRR"
     gene            3830..5272
                     /locus_tag="PLK08_05910"
     CDS             3830..5272
                     /locus_tag="PLK08_05910"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPS53068.1"
                     /translation="MFMWKIIKKLGRILRGGVAGRDIFLGVFFGFAVGMTPGMNLTML
                     IFLALLLLFNSNLVMAALATAVGRCLAIIIAPLTFELGYAMIHKMGLAGLVSSFADTP
                     VLALLDLQVYCLMGALPIIIIVGLGGAWFVSRSITKMQKAVVAASQNNEKFSKFSQNK
                     VVKFIMRLAFGKQKQELAVAMEKKSPLFNRKRIIVAVVLMVIIVGFSFCGLDWAVKKG
                     MESGIGSVNGAEVNVKNVDLSLSTGNLVIEGLQVTDPAKPEQNKVQAAKLQAKISVKD
                     LLKKQLVVDIIACDDMAMDAKRDKSGEVYLTEQQQQELASQQAAQESQTPAISGLGDT
                     KKYYDQVKKFNDQLNKLKEYLSKRSEDARQDSAVAKQQMQEDAKTQGGYFGLSASELL
                     AKHPTWLIVEGKVTKFKMAANFPSFTIEAKNVSSNPSLVKEPFSLVAKPDDTAFKDFV
                     NSTIGSNPAVKDIKEKTGGMLDKINIFKKN"
BASE COUNT         1345 a         1312 c         1516 g         1226 t
ORIGIN      
        1 gccgcagagg acgtatcaga tttcaaccac gtctgccgat gtgccgctta ttcgcaaggt
       61 actcaacgaa gccttcggcg aagccgtcga acgtcgcgtg ccgtgtacct acaatatggt
      121 gaaaaatacg aatattccgc aactcggcgt aaagacggct gatggcggcg caacgattgt
      181 gacgcctgcc ctgataaagc aggcctctgc cagcgtacgc gacgaactta tggattttga
      241 aggcggggtc atgttcgtga ttgacgacgt caaaccggct atcacggtgg ctgaccttac
      301 cggcagaata aacgatatcc gcagccagcc cgactttgaa aaacaggctg gcaatcctac
      361 ccgcgtcata ggcctcaagg gcaacggcga ggattttaac agttttgccg taatcgttcg
      421 gccggctgat gcggaaataa tgtcaaaacc tgttttacgc gaacagttcc tggacggcga
      481 acgtactgtt ttggccagcg cgctggaacg cgaagacccg accataatca ccagcttcga
      541 cgcctcgata gccaaagata ccgccagcta cgccatagta gccgtcattc tgagttggct
      601 gacgataata gtttacctct gggttcggtt cggttcggca caatggggcc ttgccgcagt
      661 gctgtgtctc gtgcacgacg tacttatagt agtcggcatg gtggcggttt ccggctggct
      721 ggccggtaca agagtaggca atgcactggg tgtaggcgtc ttcaagatag acctcaccat
      781 gattgctgcg attttgacga ttatcggcta ttcggtcaat gacaccatcg tggtttttga
      841 ccgaatacgc gaaaatcgcg gtaaactcaa ggctgtgacc gccgactgca taaatgattc
      901 catcaaccag acgcttccgc gtactttgct gacttcgttt acgacgtttt tggttgttct
      961 ggtgatgtac atattcggcg gcgacggaat gaggtcattc aactacggcc tgttgatagg
     1021 catagtcgta ggcacgtatt cttcgattgc catcgcaagc ccgctgttgc ttggttttaa
     1081 atatgccatt atcgccagga cggatgaaga tgatacatcc gaacaaaatg atgatacgca
     1141 agccgtcctg ccgcaggagt aaatgcgaat cgggccggcc tgaacggaca gttgattgcg
     1201 gatttcaaat tgcgaattga agttaagccg ttcgtctgac ttcatgatga cattcaattt
     1261 gcaaattgga attacctatt tgaaatcttt tgagttacca tgagcaaaga agcagcaaaa
     1321 cccgacatca gcgtcctgcc tgttataggc ttggaaatac acgttgagct ggcgacaaag
     1381 accaaaatgt tctgtcgttg ccggaataat ttcggcgatg cgccgaatac gaacgtctgt
     1441 cccgtatgta cgtccatgcc tggcgcactg ccggtcatga atcgtcaagc cgtcgaatac
     1501 gccatgaaag tcggccttgc gcttggctgt accgttccgt catataccaa gtgggacagg
     1561 aagaattatt attatcccga tttgcccaag ggttaccagc tcagccagta tgacctgcct
     1621 ctgtgcaagg atggcgtggt tgaatatccg cttgatggcg gcggcgtaaa accgcttcgc
     1681 gtcctgcgag cgcacctgga ggaggatgcc ggcaaaaata tgcacgatat ccctggcgct
     1741 acgggtgttg atttgaaccg tgccggcgtg cctctgcttg agatagtatc gcagccggac
     1801 atgaatacgg ttgaagaagt catgtattac gcccgcggca tgcatcggct ggtgcgatgg
     1861 ctgggcgtga gcgaagctaa catgcagatg ggccacatgc gttttgagcc gaacatcaat
     1921 cttcacataa cctggaaggg taaagtctac aagacgccga ttgtggaagt gaaaaatctc
     1981 aacagtttcc gctcgctgga aggtgcggtc aagtatgaaa tccgccgcca gtacaacgag
     2041 tggaagaatg acccggacgg tttttcgctt aaaaaactcg gcaagcagaa tcgtggatac
     2101 gacgttgaaa atgaacagac ggtttttcag cgtgacaagg aagaggcgca cgattatcgc
     2161 tatttccccg accctgacct gccggctgtt atcgttggcg acgaatggag aaatgccgtg
     2221 ttgtcgcaga taggcgaact gccgttcgca cgtcgcagtc gttatatcga tgagctcggc
     2281 atcactttcc aggaggcgga cgcacttacg caggaatcgt cgaccggtga gcttttcgac
     2341 gctgccgtaa aatccggcgc ggatgcaaaa cgctgttgca acctgctgct gggccgcggt
     2401 gcggctattg ccaatgaacg cggctgttcc atcgcggaaa taggcataac cgccgaacag
     2461 ctaggtgagc ttgcgataat gctggaaaat ggcgacgtaa atgccactgc cgcagctacg
     2521 attttcgaca aaatgctcga atcatcctgc aaatcacccc gtttgattgc cgagactgac
     2581 ggccttctta tagttcgaga cgtcgcccag ctcaacgcct gggtcgacgc cgccattgcc
     2641 gccaatccgc aggcggtcga ggaaatccgc agtggcggaa agaagaagga aaagtcattc
     2701 ggtttcctga ccggccaggt tatgcaggct tcccgcggcg cagccaacgc caaagaagtt
     2761 cagtcgctat tgaaggaaaa actttcctga attttcgtta aaaatagcgg aatttatgac
     2821 tcgacaattc acaacccgcc ttgcgccgtc accgacgggg gcgttgcatc tgggcaacgc
     2881 gcggacgttt ctggctaact ggctgctggc gaagcagcgg tcgtggcggg ttctgttccg
     2941 catggaagac ctcgacacgc ctcgcacaaa gtcatgggcg gctgacgcgg cgataaccga
     3001 attaaaatgg ctggggctcg actggggtgg cgatgttata actcaatcgc agcgaagcga
     3061 ggcttacgaa gcggcattaa aaaatctcgc agaccgcggg tcggcgtatc cgtgtaattg
     3121 cagccgccgc gacgtagaaa cttccgcatc agctccggcg gcagaagacc gcctcggcca
     3181 acaggctgcc gtttatcctg gaacttgcag aaataaaaac attgaccctg ccggagcggc
     3241 gtggcgggtg aaagtggatg atgcagtcat agagtttgac gacaatttcg ccggcccgca
     3301 gagtttcaat ctatcgcaga ccagcggtga tttcgtgatt ttccgcaaaa cgcatcaggc
     3361 ggcgtatcaa ctggccgtcg tggtggatga cgcggaatcg ggcgtggatg cgatagttcg
     3421 cggcgatgac ctgttggagt cggcggcaat gcagatgtac ctgcggcggc tgttgggata
     3481 tgatgagcgg atggagtatt ggcacttgcc gctggttctc ggcccggaca gccgtaaact
     3541 ggccaaacgc cacggcgata cgcgattgtg tcattacgtc agccttggtg ttacgcggca
     3601 gagaatgctt gggctgcttg gttattggct gggtacgctg gaaaacaggc gggaaacgga
     3661 catttccgaa ctggcggaaa aattcgatat caataaaata tccgcaaaac ctgtgatttt
     3721 cgggcgggat gaccatgatt ttctcgtcgg caggcggtaa ggcttgataa attcattttg
     3781 aaacgtaata atattataaa gatagtcaaa acattttaag gagacgaaca tgttcatgtg
     3841 gaaaatcatc aagaagctag gacggattct gcgcggcggc gttgccgggc gtgacatttt
     3901 tctgggcgtg ttttttggat ttgcggtcgg catgacgccg ggcatgaacc ttaccatgct
     3961 gatatttctg gcgttgctgc tgctgtttaa ttccaacctg gtaatggctg ccttggcgac
     4021 ggcggttggc aggtgtcttg cgattataat tgccccgctt acgttcgagc tgggttacgc
     4081 aatgatacac aagatgggcc tcgccggcct tgtgtcgagc tttgcagata cgcctgtgct
     4141 cgcactgctc gatttgcagg tgtactgcct catgggcgcg ctgccgatta tcattatcgt
     4201 cggccttggc ggcgcttggt tcgtgtcgcg cagcataacg aaaatgcaga aggctgtggt
     4261 ggctgccagc cagaataacg agaaattcag caaattttcg cagaataaag tcgtgaaatt
     4321 catcatgcgt cttgcattcg gcaagcagaa acaggaactt gccgtcgcga tggaaaagaa
     4381 atcgccgctg ttcaatcgca agcggataat cgtggcggtg gtgctgatgg ttattatcgt
     4441 cgggttctcg ttctgcggac tggattgggc ggtcaagaag ggcatggagt ccggcatagg
     4501 cagtgtgaac ggcgcggaag tgaacgtcaa aaacgtagac ctgtcgctat cgacgggcaa
     4561 tctcgttata gaaggcttgc aagtaacaga cccagccaag ccggagcaga acaaggtgca
     4621 agccgccaaa ttgcaggcaa agataagcgt gaaagacctg ctcaaaaaac agcttgtagt
     4681 tgatattata gcatgcgacg acatggcgat ggacgccaaa cgcgataagt ccggcgaagt
     4741 ctatctgacc gagcagcaac agcaggagct tgcctcgcaa caggctgccc aggaatcaca
     4801 aacgcccgct attagcggct tgggcgatac caagaaatat tatgaccagg tcaagaagtt
     4861 caatgaccag ttgaacaaac tcaaggaata tttgtccaaa cgcagcgaag acgcccgcca
     4921 ggattccgca gtcgccaagc agcagatgca agaggatgca aagacgcagg gcggatattt
     4981 cggactttcc gccagcgagc ttctggcgaa acatcccacg tggctgatag tcgagggcaa
     5041 ggttacgaag ttcaaaatgg cggcaaattt tccgagtttt accatcgagg ctaaaaatgt
     5101 atccagcaat ccgtcgctgg tgaaagaacc attcagcctc gtcgccaaac cggacgatac
     5161 agccttcaag gatttcgtga attccacaat cggcagcaat cctgccgtca aggatatcaa
     5221 ggaaaagacc ggcggcatgc tggacaagat taacattttc aagaagaatt aaccggtagt
     5281 tagctgatag ctgtccggaa aaacaaaagc gtggttggaa tttttttaaa aaatccagcc
     5341 acgctttttt agagcatgtt aaatttgaaa ttgcgccttg ttgcgtcgtt gaggacgag
//
LOCUS       DAONFI010000149         5398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17857_length_5398_cov_4.575707, whole genome shotgun sequence.
ACCESSION   DAONFI010000149 DAONFI010000000
VERSION     DAONFI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17857_length_5398_cov_4.575707"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLK08_05915"
     CDS             complement(<1..584)
                     /locus_tag="PLK08_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPS53069.1"
                     /translation="MELSITLEQPSKAATLFGPADIHLRMIREAFGIQLFARGSSIKI
                     SGAPKNVSRAAAVIDTLQRDLRQSKPITREAVADAVGESAARQQSQSESALDVYAAGQ
                     LVQAKTAGQKRYIKSVMTNDLVFCIGPAGTGKTYLAVAVAVHMLKHQQARRLILVRPA
                     VEAGEKLGFLPGDMQAKVNPYLRPLFDALHDMMD"
     gene            complement(755..1549)
                     /locus_tag="PLK08_05920"
     CDS             complement(755..1549)
                     /locus_tag="PLK08_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53070.1"
                     /translation="MNLRIGHTPTADDAYMLYGLFGGLVGSKIKYTPATASQPELNNQ
                     AACGDLDVTMLSVGAYPLLKERYEILSAGASFGKSSGPVVVTRANDRKTLDRMVVAIP
                     GATTTAYAMLQMYAPQTRTRVLPLNKLLPALEAGLADAALLIHEEFVNARHNGGLHVL
                     ADLGKWWHRTHGLPSPATVCAIKRELPAEVKKTVEADIAASVRYAFANHDTVFEAAMK
                     SAGDAHRDVIEKFIDQYVNELSVDMGLQGRKALEEFFSKICELGIV"
     gene            1744..3120
                     /locus_tag="PLK08_05925"
     CDS             1744..3120
                     /locus_tag="PLK08_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPS53071.1"
                     /translation="MSEFSSGPGYWAHVDLNRYPHLVTPEIPGPKSRALHARCTKHFK
                     GLSGQVKLFPVAFESGKGCTMKDVDGNEYIDFSSGIYVTTLGHCHPKITQAIQKAAGQ
                     LMNCHDFTTEVKTRLVEKLAQVLPGDLNGYQFYDSGTTAVEAGQRVMRAASKKNELVS
                     CFYDFHGKTYGAVSLAQIRSKCYGPCRAPGMHLVPRPDTYRPIWTKADGTIDTDKYIE
                     FYEMYLDRATINDVAGFILEPIQGWGGSVMPPDDFFPKLRAMCDRRDIMLMADEVLTS
                     WGRTGKFLCMEHWNVLPDVVSIGKGFGNGFPVTCLAVREKYCEAFESISASSSYGGNP
                     MACAAALASIEVIEEENLLERSLHLGELAMKRMLKFKENYKIVGDVRCKGCLMGIELV
                     KDKSTKEPFQEAGVSVYQKAFRKGLAWIPAGHILRMSPPIIMEDEVLLRGMDMIEEAI
                     AETEKELL"
     gene            3187..4329
                     /locus_tag="PLK08_05930"
     CDS             3187..4329
                     /locus_tag="PLK08_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS53072.1"
                     /translation="MRTVKFGIIGCGLMGREFASACARWFHLLDMKVRPEIVAICDTS
                     DDLLRWYADNIPTIRQVTKNYKELLANDEVQAVYIAVPHNLHQEFFCASVEAGKHLLG
                     EKPFGMDEKANMAIMECCKRHPDVFVRCSSESIFFPAMQKIGALIESGGLGKIIEANS
                     GFLHSSDLDFTKPINWKRMLKFNGEYGCMGDLGMHACHMPFRAGWIPQNVRAVLSDIV
                     KERPDGRGGMAPCETWDNATLLCETKDSAGETFPMTIRTHRIAPGNKNTWYFEVYGTR
                     ASARWSSRQINTLEVLKYDGGEQSWQVTDMGHEMAFKSLTGGIFEAGFSDSILQMWAA
                     FLYELTEGRPKSRFAGCVTPQEVSVSHRLFTSALESQANQTTVSLW"
     gene            4478..4807
                     /locus_tag="PLK08_05935"
     CDS             4478..4807
                     /locus_tag="PLK08_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53073.1"
                     /translation="MRSSKKVVVLLLICVLLFFGKNALSFCAHGEAGSATAYADVPHI
                     VLCAFKISKSNGRCRENGVGQILQAVGLLICADMSLPYIFILSKSNSASLKIASSLHG
                     MGCRLQI"
     gene            4898..5353
                     /locus_tag="PLK08_05940"
     CDS             4898..5353
                     /locus_tag="PLK08_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53074.1"
                     /translation="MLCDKDLLVQARKMMEDDDLQAAKKILDGLNNKSIWVQNASAVW
                     LMRTGRTDEAVRLLSQLVFPGGTSIMGLGVPDKIKLNLAEAMLLKGNISGAMSLIKSS
                     DGGDPHCRKLVAAVDRWRKTLPLFSRMAVICGVMPYESEIFIDSPYGEP"
BASE COUNT         1283 a         1329 c         1445 g         1341 t
ORIGIN      
        1 taatccatca tatcgtgcag ggcgtcgaat agcggccgca gatatggatt gactttagcc
       61 tgcatatcgc cgggcagaaa tccgagtttt tcaccagcct ccaccgccgg ccgtacaagt
      121 atcagccgcc ttgcctgctg gtgtttgagc atatgtactg ctaccgcgac ggcaagatac
      181 gtcttgcctg tgccggccgg cccgatgcag aataccaggt cgttcgtcat caccgatttg
      241 atgtatcttt tctggccggc tgtctttgcc tgaaccagtt ggccggcggc atagacatca
      301 agggctgatt cgctttgtga ttgctgacgc gcggctgatt cgcctacggc gtcggctacc
      361 gcttcccgcg tgattggctt gctctggcga aggtctcgct gcagcgtatc tataactgcc
      421 gccgcacgcg aaacgttttt gggcgctccg cttatcttga tgctcgagcc gcgggcgaaa
      481 agctgtatgc cgaacgcttc ccgtatcatt ctgaggtgaa tatcggctgg gccaaagagt
      541 gtcgctgctt ttgagggctg ttcgagtgtt atgctcaatt ccatttttta gcctgtttta
      601 gcttgtgtaa cttgtgtaaa aatccttaaa atccatttgc cccggcaata ttatgatttt
      661 ctcaaggtga cagattcgca ttattctgtc ttttcccaaa tatactctgc aattcgattc
      721 accgtttgcc attatacagg tcggcttgaa aaaatcatac tattcccagc tcacatattt
      781 tcgaaaaaaa ttcttccagg gctttgcggc cctgaagccc catatccacc gaaagttcat
      841 ttacatattg gtctatgaat ttttcaatga catcgcgatg tgcgtcgccg gcggatttca
      901 tggctgcttc aaaaaccgta tcgtggttgg cgaaggcgta tctgacggat gcggcgatat
      961 cggcttcgac ggttttcttt acctcagccg gcagttcgcg tttgattgcg cataccgtag
     1021 ccggcgacgg caggccatga gtcctgtgcc accatttgcc caggtctgcc agcacatgta
     1081 aaccgccgtt gtgccgggca ttcacgaatt cctcgtgtat caggagtgcg gcgtcagcaa
     1141 ggccggcttc cagcgcgggc agaagtttat tcagcggcag aaccctggtt cgcgtctgcg
     1201 gagcgtacat ctgaagcatg gcgtaagccg tcgtggttgc gccggggatg gctaccacca
     1261 tcctgtccag agttttgcgg tcattggcac gcgtaactac aacgggccct gaagatttac
     1321 cgaaagatgc gccggcagag agaatctcgt atcgctcctt caatagcgga tacgcaccca
     1381 cgctgagcat ggtgacgtcc aaatcgccac acgcagcctg attgttcaac tccggctgcg
     1441 atgccgtcgc gggggtatat ttgattttag aacccaccaa gccgccaaaa aggccataaa
     1501 gcatataggc gtcatcggca gtgggggtgt ggccgattcg cagattcatg tttttatcat
     1561 atcaaatcac agcgacaata acaaacggct gccggcgact attgtggaaa atattatgcc
     1621 acgactcaga atatgcaaaa atgagccgcc tgccggaatt tctcttgaca aaatgttaat
     1681 ttgtgttagt tttaagataa agataacaaa tactatcatt tttgacaata caggagttta
     1741 gtcatgtcag aattttcaag cggccctggt tactgggcac acgtcgattt gaacaggtat
     1801 ccgcatcttg ttacgccgga aattcccggc ccgaaatcca gagcgttgca cgcccgttgc
     1861 acgaagcatt ttaagggcct ttccggccag gttaagctgt ttccggtagc cttcgagagc
     1921 ggtaagggct gtaccatgaa agatgtggac ggaaatgaat atatcgattt ttcgtcgggc
     1981 atatatgtta cgactctcgg ccactgtcac ccgaagatta cgcaggccat ccagaaagcc
     2041 gccggccaac tgatgaactg tcacgatttt acgactgaag tcaaaacccg tctggttgaa
     2101 aaacttgcac aggttctgcc tggcgatctc aacgggtatc agttttacga ttccggcacg
     2161 acagccgtcg aagccggcca gcgagtcatg cgagccgcca gcaaaaaaaa cgaactcgta
     2221 agctgctttt acgattttca cggtaaaact tacggtgcgg tatcgctggc tcagattcgt
     2281 tcaaagtgtt acggcccttg tcgtgcacct ggcatgcatc tggtgcccag gccggatacg
     2341 tatcggccaa tatggacgaa ggctgacggc acgatagata ccgacaaata catcgaattt
     2401 tacgaaatgt atcttgaccg cgcgacaatc aatgacgtcg ccggattcat tctcgaaccc
     2461 atccagggct ggggcgggtc ggtaatgccg ccggacgatt ttttccccaa acttcgcgcg
     2521 atgtgcgacc gcagggacat aatgctgatg gctgacgagg tgctcacgag ttggggccgt
     2581 acgggcaaat tcctgtgcat ggagcactgg aacgtgctgc cggatgtagt aagtatcggc
     2641 aaggggttcg gcaacggttt tccggtgaca tgcctggctg ttcgcgaaaa gtactgtgaa
     2701 gcgttcgagt cgataagtgc atcgagcagc tatggcggca atccgatggc gtgtgcagcc
     2761 gcgctggcga gcatcgaggt tatcgaagag gaaaaccttc tcgaacgttc actgcatctc
     2821 ggcgaactgg ccatgaaacg aatgcttaaa ttcaaggaga actacaaaat agtcggcgat
     2881 gtccgttgca aaggctgcct catgggcatt gaacttgtaa aggacaaatc gaccaaagag
     2941 ccgttccagg aagccggcgt cagcgtctac cagaaagcct tccgcaaggg tttggcgtgg
     3001 atacccgccg gccatatcct tcgcatgagc ccgccgatta tcatggaaga cgaagtgctc
     3061 ctgcgcggca tggatatgat tgaagaagcc atcgctgaaa ctgaaaagga actgctttaa
     3121 tcggtttttt ctatgccctg ctatgcaggg agatgttcgt tggctggatt tttttaagga
     3181 tatcgtatga gaacggttaa attcgggatt atcggctgcg gcttgatggg ccgcgagttt
     3241 gcgtcggcat gtgcccgctg gtttcatctg ctggacatga aggtacggcc tgaaatcgta
     3301 gccatctgcg acacgagcga cgacctgctc cggtggtatg ccgacaatat tcccacaatc
     3361 aggcaggtta cgaaaaatta taaggaactg ttggccaacg acgaagtgca agccgtctac
     3421 attgccgtac ctcacaacct gcaccaggaa ttcttttgtg catccgtcga agccggcaag
     3481 catctgcttg gcgaaaaacc gttcggcatg gatgaaaaag cgaacatggc gattatggaa
     3541 tgctgcaaac ggcatccgga tgtgtttgtc cgctgttcca gcgaatcgat attttttccg
     3601 gccatgcaga agataggggc gttaatcgaa tccggcggcc tgggaaagat aatcgaggcc
     3661 aacagcggtt tccttcactc cagcgacctg gatttcacca agcccattaa ttggaagcgt
     3721 atgttgaagt ttaatggcga atacggctgt atgggcgatt tgggcatgca tgcgtgtcac
     3781 atgccgtttc gtgccggctg gataccgcag aacgttcgtg cagtgttgag cgacatcgtg
     3841 aaagaaaggc cagacggcag gggcggtatg gcgccgtgcg aaacgtggga taacgctacg
     3901 ctgctgtgtg aaaccaaaga ttctgccggc gaaaccttcc cgatgacaat ccgcacgcat
     3961 cgcattgcac ctggcaacaa aaatacgtgg tattttgaag tgtatggcac aagagcgtcg
     4021 gctcgctggt cgagcaggca gataaatacg ctggaagtgc ttaaatacga cggcggagaa
     4081 cagtcgtggc aggtgacgga tatgggccac gagatggcct tcaaaagtct caccggcggc
     4141 atattcgagg cgggctttag tgactctatt ctgcaaatgt gggctgcttt cctatatgaa
     4201 cttaccgaag gcaggccaaa gagcagattt gccggctgtg tgacaccgca ggaagtgtcc
     4261 gtcagccaca gactctttac gtcggctctt gagtcgcaag ccaaccaaac gactgtttct
     4321 ctctggtaat ttttccccaa aaaatattag cgcttcaacc ggcgtctgcc caatcgcagc
     4381 cgccggtttt tttatgtgcg ctgttccgga aagatgggat ttgtcaggca atttacccat
     4441 attgccaacc tggccagcag gggatataat atgacccatg cgttcgtcga aaaaagtcgt
     4501 cgtcttgctt ttgatatgcg ttctgctctt ttttgggaaa aacgcattgt cgttctgtgc
     4561 gcatggcgag gctggttctg ccaccgccta tgcagatgtg ccccatattg tcctgtgcgc
     4621 ttttaaaatt tccaagtcga acggccgttg cagggaaaac ggcgtcggcc agattttaca
     4681 ggctgtcggc ctgttgattt gtgccgacat gagcctgcca tacattttca tactttcaaa
     4741 atcaaattca gcctcactta aaattgcgtc cagcctgcat ggcatgggat gcaggctgca
     4801 gatttgaact ctcgattact gtgcagttgc cggagttgaa ggcttacgta ttttgcgtca
     4861 gccggttctg tttttcagca cgtaatggag agtgaaaatg ttatgtgaca aggatttgct
     4921 ggtgcaggct agaaaaatga tggaagatga cgacctgcag gcggctaaaa aaatacttga
     4981 tgggttgaat aataaatcga tatgggttca gaacgccagc gcggtgtggc tgatgcgaac
     5041 gggccgaacg gatgaagccg tccgactatt atcccaactt gttttccctg gcggcacatc
     5101 gattatggga ctaggcgtgc ccgacaagat aaaacttaac ctcgccgaag ccatgcttct
     5161 gaagggcaat atttccggcg ccatgtcgct gataaaaagt tccgacggcg gcgacccgca
     5221 ttgtagaaaa ctcgtcgccg cggttgacag atggcgaaaa acgctgccgc ttttttctcg
     5281 catggcggta atatgcggcg tcatgccgta tgaaagcgag attttcatcg attcccctta
     5341 tggagaacct tgaataatat ctgcaaaatt gattcggaag tttatagtta aggccagt
//
LOCUS       DAONFI010000150         5378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_17945_length_5378_cov_3.831110, whole genome shotgun sequence.
ACCESSION   DAONFI010000150 DAONFI010000000
VERSION     DAONFI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17945_length_5378_cov_3.831110"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLK08_05945"
     CDS             complement(<1..444)
                     /locus_tag="PLK08_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53075.1"
                     /translation="MSVYFNSAIQVNTLKIFNGNSSSSIKKALKTVVVKAVRSVEKTI
                     SCVALAGIVLGFAADRANANYSFDLNSEISIKTVYSFNELCFSSTDGGVLVMDDENGG
                     VTINGQMPADYGSVQMGFSLLNAGVITFVGYASNDDTSTSHPEWGS"
     gene            995..1846
                     /gene="rpsB"
                     /locus_tag="PLK08_05950"
     CDS             995..1846
                     /gene="rpsB"
                     /locus_tag="PLK08_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPS53076.1"
                     /translation="MSTELVKKLIDAGIHFGHRTSRWNPKMKPYIHGKRNNLYIIDVK
                     ETLKGLLVAKKFVTQIVAGGKDVLFIGTKRQARKAVLNTAEKTGMHSVIDRWLGGTLT
                     NFREIRKRVGRLIELETMEAEGSLDAFSKKDGSRLRREMKKIKRNLGGIRNMERFPGV
                     MFVIDEPNEVIAVQEARKAGIPVISLMDTDGDPDLVDIPIPGNDDSMRAIEVVLEEIV
                     KAIAEGMTGRAEAAAQEAAKAAEDAANKDRRHRRHTSDADDAQMIAAAEKVAAADETI
                     AAEKAAE"
     gene            1893..2687
                     /gene="tsf"
                     /locus_tag="PLK08_05955"
     CDS             1893..2687
                     /gene="tsf"
                     /locus_tag="PLK08_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPS53077.1"
                     /translation="MAVTAAVVKELRESTGLGMMDCKKALDETNGDIQAAKDLLRKKG
                     LATAEKKAGRATKEGLICMNFAADGTSATMIEVQCETDFCARNEEFVKMVNDLAEMAA
                     SASAGKIEASDKMTARLQETLAKIGENMNFARGIKITAPKTTSYLHHNKKVGVIVGYD
                     GELDAETAAGLCQHIAFANPMGLVAEDIPAAVIEKEREFARQEAIDSGKPAEIAEKMV
                     EGKIRKFVAERVLLEQNYVKDEKKKIKDILGKAKVVAFARFAVGDM"
     gene            2796..3512
                     /gene="pyrH"
                     /locus_tag="PLK08_05960"
     CDS             2796..3512
                     /gene="pyrH"
                     /locus_tag="PLK08_05960"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007341377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPS53078.1"
                     /translation="MKDIKYKRVLLKLSGESLSRPGELGVDGQSVADIVARLVPTIRM
                     GVQMGIVIGAGNLMRGRHLTDASNIHRTTADYMGMLGTVMNALALRDGLQHAGCDAVA
                     VSAISMPLICDLYQRPRVMRHLAEGKVVIFAGGTSHPGVTTDMCAAIRASDIEADVLL
                     KATKVDGVYDSDPKKNPAAKKFDTITCEQAIALKLGVMDLPAMAFCMERKIPIMVFNM
                     NIDGHLEQAVRGKNVGTIVE"
     gene            3527..4081
                     /gene="frr"
                     /locus_tag="PLK08_05965"
     CDS             3527..4081
                     /gene="frr"
                     /locus_tag="PLK08_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPS53079.1"
                     /translation="MDEILLTVEENMDSALEFLKKELRSLRTGRASTSLVDKLMVNYY
                     GSPTELRQMATISTPEATQILIKPFDQSSLKEIERAIQASDLGLNPSNDGKVIRLNIP
                     PLSIERRNQLTSQVKKMAEQARVTIRNARRDGNKEVDTAQKATTLTEDDAKKGKDKIQ
                     KLTNDYEAKVTEILEAKTKEIMES"
     gene            complement(4229..>5378)
                     /locus_tag="PLK08_05970"
     CDS             complement(4229..>5378)
                     /locus_tag="PLK08_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53080.1"
                     /translation="KLKEVLGQPSWVMSTDNVKMAISQLGGQLAPVIFFADSKTPVQP
                     YWVAPWYRIKDMKFPRTDTVLKPLRGDFFCLPFGANAEKYEGEQHTCHGEAGTEKWKY
                     VNLCDDGKIVKLTLSMNTKIRKGRIVKDTWLKNGHNAVYQSHELSGYAGKIPLANHHT
                     LAMSDTPESVLVSMSSLKMGFTNPVAGDIDKYDYAFVATNEKFRDLAKVPTRFKTPAT
                     CDCSKLPTRKGYGDLVQCFHNPSDRISWVTATYTSEGFMWYSLKLTEQYPAVTVWLFN
                     NNRHNYPWDIFEDACLGLENSCSYLACGLADSVRPNPASRAGFKTYATFSPKTPSRIN
                     IIQGVVRVPAGFGKATSAKFDKGKVTFISGTKKAVAKVDWEFLENGSL"
BASE COUNT         1531 a         1395 c         1322 g         1130 t
ORIGIN      
        1 actaccccat tcaggatgcg acgtactagt atcatcatta cttgcataac cgacaaaagt
       61 tatcactcca gcattaagca atgaaaaacc catttgaact gagccataat cagccggcat
      121 ctgcccgtta atcgtaacgc ccccattctc atcatccatc accaatacac cgccatcggt
      181 actggaaaag cacaattcat tgaaactata tacagtcttt atgcttattt cggaattaag
      241 gtcgaaactg taatttgcat tcgccctgtc ggctgcaaaa ccaagtacaa tgccggctaa
      301 cgcgacacac gagattgttt tttctacgga acgtacagcc tttacaacga ctgttttcaa
      361 tgctttttta atactagatg aagagttacc attaaaaatc tttagggtat tcacctgaat
      421 ggcagaatta aaatagactg acatttcaaa aatcctttcc aaaaaataag caaacaccaa
      481 tctttatccg tcgagaatga acgggggatg aaaaaaccaa accctcatag gcggaagcat
      541 ttcagctcaa aatgctgccc taatattatc caaccataca aataacgtca agctgtttgc
      601 aaaaattttt tgagggaatt tacaaataaa caccttaaat attggaatta taaggaataa
      661 tcccacaaca attttataaa atgaatctcc gtagccgccg ccaacagccg ccgtctactc
      721 tcagccaaaa taatgatgaa acaaaaaccg tcatttcgag gaaattttgg ctgaaatcgg
      781 caaaaattgt tgacttgtcg gtattataaa cgtattttat tgctcttttg cggcattcag
      841 ctgcccgccg gcgtaggcgg gaagacggca aaatgctaaa aaaacgttcc gcccgacgga
      901 acataatacg gttcagtccg cccgacttac ctgccttgat atgaggtttt aaaggtacga
      961 aagatggacg gctgagtcat agcaggagat ttccatgtct acagagttgg tcaagaaact
     1021 catcgacgcg ggcatccatt tcggacaccg caccagccgc tggaatccaa agatgaaacc
     1081 ctatatccac ggcaaacgca acaatcttta catcatcgac gtaaaggaaa ctctcaaagg
     1141 cctgctggtt gcgaagaaat tcgtcacgca gatagtagcc ggcggcaagg acgtgctgtt
     1201 catcggcact aaacggcaag cccgcaaagc cgtcctcaac acagctgaaa aaaccggcat
     1261 gcacagcgta atcgaccgct ggctgggcgg cacgctgact aattttcgcg aaatccgcaa
     1321 gcgtgtaggc cgtttaatcg agctggagac gatggaagcc gaaggctcac tcgacgcatt
     1381 cagcaagaag gacggctcac ggctgcgtcg cgagatgaag aaaatcaagc gtaatctggg
     1441 cggcatccgc aatatggagc gtttccctgg cgtgatgttc gtaatcgacg aacccaatga
     1501 ggttatcgcc gtccaggaag cccgcaaagc cggcattccc gtgatatcgc tcatggatac
     1561 cgacggcgat ccggatctgg tggatattcc gatacccggc aacgacgact ccatgagagc
     1621 catcgaggtc gtgttggaag aaatcgtcaa agccatcgcc gaaggcatga ccggccgggc
     1681 tgaagccgcc gcgcaggaag ccgccaaagc cgctgaagac gccgccaaca aagaccgccg
     1741 ccatcgccgt cacacctccg acgccgacga cgcacagatg atagccgccg ccgaaaaggt
     1801 cgcagccgcc gatgaaacca tcgccgccga aaaggctgcc gagtgagttt catccctgaa
     1861 cgaagttaac tttaatcgca aggagattca caatggctgt aaccgcagca gttgtcaaag
     1921 aattacgtga aagcacgggc ctgggcatga tggattgcaa aaaggctctg gacgaaacca
     1981 acggcgacat tcaagccgcc aaagacctgc ttcgcaaaaa agggttagcc accgccgaaa
     2041 agaaggctgg ccgggcaacc aaggaaggtt taatctgcat gaacttcgcc gcggacggca
     2101 catcggcaac gatgattgaa gtgcagtgcg agaccgactt ctgtgcccgc aacgaggaat
     2161 tcgtgaaaat ggtcaacgac cttgcggaga tggcggctag cgcttctgcc ggcaaaattg
     2221 aagcatcgga taaaatgaca gcccgcctgc aggaaacgct ggcgaaaatc ggcgaaaaca
     2281 tgaacttcgc ccgcggcata aagataactg ccccaaaaac cacttcttac ctgcatcaca
     2341 acaaaaaggt cggcgtgata gtgggctatg atggcgagct tgacgcggaa accgccgcag
     2401 gcctctgcca gcatatcgcc ttcgcaaacc cgatgggcct cgtcgcagaa gatataccgg
     2461 cggcagtcat cgaaaaggaa cgcgaattcg caaggcagga ggctatcgac tccggcaagc
     2521 cggctgaaat cgccgaaaaa atggttgaag gcaaaatccg taaattcgtc gccgaacgcg
     2581 tacttctgga acagaattac gtcaaggacg aaaagaaaaa aatcaaagac atactgggca
     2641 aggctaaagt tgtggctttt gcccgtttcg cggtgggcga tatgtaacgg atacgctcct
     2701 agaacacaaa acggcgggtc gaaacaaacg gcctgccgtt tttttatgtc gtttacctgt
     2761 tgcagtaaag ccgtcgggag tttaatatct cagacatgaa agacatcaaa tataaaaggg
     2821 tactgctcaa gttatccggc gagtcgctgt cgcggcccgg cgagcttgga gtggacggcc
     2881 aatcggtggc ggacatcgtc gcacgtcttg tgccgacaat cagaatgggc gtacagatgg
     2941 gcatagtaat aggcgccggc aacctgatgc gcggccgcca tttaaccgac gccagcaata
     3001 ttcatcgcac gacggcagac tatatgggca tgctcggcac ggtcatgaat gcgttggcct
     3061 tgcgcgacgg cttgcagcac gccggctgcg acgccgtagc ggtgagcgcc atatccatgc
     3121 cgctcatatg cgacctttat caacggccac gcgtcatgcg acatctggca gagggcaaag
     3181 tggtcatttt cgccggcgga acgagtcatc caggcgtaac gacggatatg tgcgcggcta
     3241 tccgcgcttc ggacatagag gctgacgtac tgctaaaggc gaccaaggtc gacggcgtat
     3301 acgacagcga ccccaaaaaa aatccggcgg caaaaaaatt cgacacgata acctgcgaac
     3361 aggctatcgc gttgaaactc ggcgtaatgg atctgccggc gatggcgttc tgcatggaac
     3421 ggaagattcc gattatggtc ttcaacatga atatcgacgg ccatctcgaa caggccgtac
     3481 ggggcaaaaa cgtaggcact atcgtcgaat gaaaggataa ccgaacatgg acgaaatact
     3541 tttgacagtg gaagaaaata tggacagcgc gttggagttt cttaaaaagg agctccgctc
     3601 gctacgcaca ggcagagcat cgacgtcact ggtggacaag ctgatggtca actactacgg
     3661 ctcaccgacc gagctgcgcc agatggccac catctctacc cccgaagcca cgcagatact
     3721 cataaaaccc ttcgatcagt cgagcctcaa ggaaatcgag cgggctatcc aggccagcga
     3781 cctggggctg aaccccagca acgacggcaa ggtgattcgg ctgaacattc cgccgctttc
     3841 catagagcgt cgcaaccagc tcacatcgca ggttaaaaag atggcggaac aggctcgcgt
     3901 aacgattcgt aacgcacgcc gcgacggcaa caaggaagtg gacacagcgc agaaagcaac
     3961 aaccctaacc gaagacgacg ccaaaaaggg caaagacaaa atccagaaat taaccaatga
     4021 ttacgaggct aaagttaccg aaattctcga agccaaaacg aaagaaatca tggaatcata
     4081 acaaagccgt ttcacacaat aaaaaggctg gccgaaattt tttaagaaaa ctccggccag
     4141 ctttttttat taaaaaaatc tcaagaaaca attttcaagt aacaagcaaa ctcaaaattt
     4201 caattttcaa ttcgtaattg ccaattttct acaggctgcc gttttccaaa aattcccagt
     4261 caactttggc cacagccttc tttgtgccgg aaataaaggt cactttgccc ttgtcgaatt
     4321 ttgcggaagt tgctttccca aaaccagccg gcacacgcac gactccctgt attatgttta
     4381 tgcgcgacgg cgtcttggga gaaaacgtcg catacgtctt gaaacccgcc ctgcttgctg
     4441 gattcggccg gacactgtca gccagcccgc atgccagata gctgcacgaa ttttcaagcc
     4501 ccagacacgc atcttcgaaa atatcccacg gatagttgtg gcgattgttg ttgaacagcc
     4561 agacagtaac ggcgggatat tgctccgtga gttttaacga ataccacata aaaccctcgc
     4621 ttgtataggt agccgtcacc catgaaatcc tgtccgaagg attgtggaaa cactgaacca
     4681 ggtcgccgta gcctttgcgg gtcggaagtt ttgaacagtc gcaggtcgcg ggcgttttaa
     4741 atcgcgtggg aactttggcc aaatccctga acttttcatt cgtggcaacg aaggcgtaat
     4801 cgtatttgtc gatatcgccg gccacagggt tggtgaaacc catttttaga gaactcatcg
     4861 ataccagcac gctttcgggc gtatcggaca tcgccagcgt atggtggttc gccaaaggaa
     4921 ttttgcccgc ataaccggaa agttcgtgcg actgatagac tgcgttatgt ccatttttca
     4981 gccatgtatc cttgacgatt ctgcccttgc ggattttggt attcatcgaa agcgtcagct
     5041 tcacaatttt gccgtcgtca cataaattaa cgtatttcca cttctccgtg ccggcttcgc
     5101 cgtgacaggt atgctgttcg ccttcatatt tttcggcatt agccccgaac gggaggcaga
     5161 aaaaatcgcc gcgcaacggc ttgagcacgg tatccgtgcg ggggaatttc atgtctttta
     5221 tcctgtacca cggggcaacc caatacggct gaacgggggt tttgctgtcg gcgaaaaata
     5281 tcaccggcgc aagctggccg ccgagctgcg atatcgccat cttgacgtta tcagtagaca
     5341 tcacccagga cggctggccg agaacttctt tgagttta
//
LOCUS       DAONFI010000151         5339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18123_length_5339_cov_4.008327, whole genome shotgun sequence.
ACCESSION   DAONFI010000151 DAONFI010000000
VERSION     DAONFI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18123_length_5339_cov_4.008327"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="PLK08_05975"
     CDS             complement(<1..1707)
                     /locus_tag="PLK08_05975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPS53081.1"
                     /translation="MKIRTKFLLIMLLLATMPALMVSSLIIYYLGRTQQQVARGAYSV
                     LSEDAMESLRTIVNNFGQQFNSQMVALRLTLDNQAMSVMERLNGPVPARHPAILFPGQ
                     PIADGQQVFYVAPDVRLNQEIDGDMARLADMAGVYEKLQTANASIITSQYTALTNGTY
                     CSFPGKGKTFPDGFIPTLRPWFEEAKKSQELRWLFMSDAITKKFIITGVLPLWDGNGN
                     FVGATALDADMSVLLDMMQLPESWSESAMVMLLHVTRDVSGEENETVVLRREHGVFDP
                     TPIPLGKLFASCKKPKASNDLKNSSGVLREISGFLRNDVGGVAEICYGGEKFICAVGR
                     QGKNGIVPMVIIPQNVITADAEESIGLIRKTTGYILEYSSAGMLPIMLLAVGLAWFIS
                     RRITSPIHKLVMASKKLAYGDFSARVDIRTKDEMSLLGDVFNQMGPQLQDREQIKHSL
                     LLASEVQHNLLPAESPDVPGFDIFGRSDYCDETGGDYFDFIPLNNGQLAVAVGDITGH
                     GVGAALLMATARALIRSHAFHHDCTLGKMMEDVNVHMVHDTGDSRFMTLFFGVINPHS
                     REI"
     gene            complement(2074..2268)
                     /locus_tag="PLK08_05980"
     CDS             complement(2074..2268)
                     /locus_tag="PLK08_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53082.1"
                     /translation="MTDNQQAVENKADCSKNASGGKIWTVGTLSYTTKGLLILFGWLM
                     IEVFFITSSQNTTGMPVDEC"
     gene            complement(2522..4972)
                     /locus_tag="PLK08_05985"
     CDS             complement(2522..4972)
                     /locus_tag="PLK08_05985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HPS53083.1"
                     /translation="MMTAQTQIHYSGEINNHTGMALGGLGTGTLAIDHAGRFQDICVQ
                     NNWRTKRQVSPAGSFFAIHTESPQKSSGKVLQLTQEGSLPCIDKLDYCGDFPFLNIDY
                     TDKELPCDVSMEAFSPMVPHHADISSLPLVFFKFKVKNTSKTAIKAAIAFSWQNDISV
                     WMHHKNAPLGPLLASNGKYNTLLENDDNPAVLMSTYEELLKNSEYMLTGIKTDGVKFS
                     AVADWWNIPLKCNDFMNDNPEIVKLRNTTDKALALWRSFLERGELPAERNFDDKLGKY
                     SYHSPAGAVAGEIELAPAETKEICFALLWYFPYHLDGTGHYIGHEYARRFPDGTQQIA
                     SKAIPDYEALRSKTGSWKPMIANASLPEKTLKFINSSLYLLPRITWYMENGNFTYYES
                     LDCPRMFCIVLEQCAAPLMAAFFPELHAKALELFVSLQRESGEIPTTLGFFESIYTPE
                     FRVFSPNDVAAFPLSVYTNIVFGADTAFAKKMYPKVKRAMQWGKSLDVDNDGIPDCHG
                     FDQGWDTWPMSGTVSYLSDLWLAALKAGIKMAKSFKDEEFANWCSDAFKKASDTIETK
                     LWNGKYYNLFYNRTNGTSSDTSAIDQFAGQTWANILGLGDLHPRERIKTAVKNILKNN
                     IESAGKYPCSGVKDGGIPDCSSTHNKQSRAFVPCFIGSFSANAMCYGEFEKAVNAMEQ
                     AADAIINKEEEPWLGQLLFDSADGKHFYGVHYLDMLIIWDVMHALAGAKVDPFNETLT
                     LCPCEIPSRGPVFSNLFWGEVEYKENKIILHNHSDIKAEFKRIIFRHPQETEDAETFE
                     NVVINPKSFLCLDIRLSN"
BASE COUNT         1353 a         1268 c         1206 g         1512 t
ORIGIN      
        1 gatttcacgg ctgtgcggat ttataacgcc gaaaaacagc gtcatgaacc tggaatcgcc
       61 ggtatcatgc accatgtgaa cgtttacatc ttccatcatt ttgcccaacg tgcagtcgtg
      121 atggaacgca tggctgcgta ttagcgcacg ggcagtggcc atgagcaacg ccgcgcctac
      181 gccgtggccg gtgatgtcgc caactgccac cgcaagctgc ccattgttca gcggaatgaa
      241 atcaaaataa tcgccgccgg tttcatcaca atagtcactg cgtccaaaaa tgtcgaaacc
      301 cggcacatcg ggcgattcgg cgggcagaag attgtgctgt acctcgctgg ccagcaggag
      361 cgaatgcttt atctgctccc tgtcctgaag ttgagggccc atctgattga acacgtcgcc
      421 cagcaggctc atctcgtctt tggtgcggat gtcaacccgt gccgaaaaat cgccgtaggc
      481 aagttttttc gacgccatca ccagcttgtg aatcgggctg gttatgcgtc gactgatgaa
      541 ccacgcaagg ccgacagcca gcagcattat cggcagcatg ccggctgatg aatattccag
      601 gatataaccg gttgtcttgc ggattaaacc tatgctttcc tccgcatcgg cagtaatcac
      661 attttgcgga attataacca tcggcactat gccgtttttg ccctggcggc cgacggcaca
      721 gatgaatttc tctccgccgt aacaaatctc cgctacgccg ccgacatcgt ttcgcaaaaa
      781 tccgcttatc tccctcaaaa cacctgagga attctttaaa tcgtttgacg ctttgggttt
      841 tttgcaactt gcaaacaatt ttcccaacgg tatcggggtc gggtcgaaaa cgccgtgttc
      901 acgtctcaaa acgacggttt cattctcttc gccgctgacg tccctggtaa cgtgcaataa
      961 catgaccatg gcggattcag accatgattc gggcaattgc atcatgtcca gcagtacgga
     1021 catgtccgca tccagcgcag ttgcgccgac gaaatttcca tttccgtccc acaggggcaa
     1081 gacgccggta atgatgaact ttttcgtgat ggcgtccgac atgaacagcc atcgtaattc
     1141 ctgcgatttt ttggcttcct cgaaccacgg ccgcaatgta gggataaatc cgtccggaaa
     1201 tgttttgccc tttccaggga aactgcaata cgtcccgttc gtcaaggctg tatactggct
     1261 ggtaattatt gaagcgttgg ccgtctgcag tttttcatat acgccggcca tgtcggcgag
     1321 gcgggccatg tcgccatcta tttcctgatt cagcctgacg tccggtgcaa cataaaatac
     1381 ttgttggccg tcagctatgg gctggccggg aaaaagtatc gcaggatgcc tggctggcac
     1441 tggcccgtta agacgctcca tgacagacat ggcctgattg tccagcgtca gacgcaaggc
     1501 taccatctga ctgttaaact gctggccgaa attgttcaca atggttctca gagattccat
     1561 ggcgtcttcg ctcaatacgg aatacgcacc tctggcgacc tgttgctggg tacggccgag
     1621 atagtatatt ataaggctgg agaccatgag tgcaggcatt gtcgccaaca gtaacattat
     1681 cagcagaaat ttagtccgta ttttcatgga taagtccctg aatagccgac agtttcataa
     1741 tcacttcaga cgcgggattc catcggctgt tcattaatct gcggtatatt cttgcataat
     1801 ccccaaaatt tctcaaatca aaaaatccgg caaaaacgtt atggtttgta caaagtagca
     1861 ggttatggtg gctgcttatt gataaatatg ctgcatcatc gagataacca tagaattttg
     1921 agatattcgc aatggaattg aacgtagata ccccagggca agccaggggc acttactgtt
     1981 acagttgcta acgcaactga cctgaatctt gccgcatgca cattatttcg tgcaagcatc
     2041 aatattcagg aatgacgacg gcttgtgccg tcgtcagcat tcatccacgg gcatgcccgt
     2101 ggtattctgt gaggaagtaa taaaaaatac ctctatcatc aaccagccga aaagtatcag
     2161 caggcctttg gtcgtataac ttagcgttcc gacagtccat atttttccgc cggatgcgtt
     2221 tttcgaacag tcggctttat tttcaactgc ctgctgattg tcagtcatgc tctcaccata
     2281 tgtatgctgc ctgtacatct gaacattgtt catttataca tggagtttca aggctggtcg
     2341 cttcattttc tatagctgat tctgtttcca tgataagtca tctccatctt ttaaagaaca
     2401 aacataaact gggcgtccca aatctataat agagcagaca tgtccgatta cattgagctc
     2461 cagagctcaa gcagacagaa tgcatcaaaa ctttttcatc gatttcataa gtttacctgc
     2521 ctcagttgga caaacgtata tccaggcata ggaaactttt ggggtttatt acaacatttt
     2581 cgaaggtttc tgcatcttcc gtttcttgcg ggtgacggaa tatgattctt ttaaattcgg
     2641 cttttatgtc ggagtggtta tgcagaataa ttttattttc tttatattcc acttcgcccc
     2701 agaaaaggtt tgagaaaaca gggcctcttg acggaatctc acatggacac agtgtcaatg
     2761 tttcgttaaa tgggtcaact ttcgctcctg caagggcatg cattacatcc catattatca
     2821 gcatgtcaag ataatggacg ccgtaaaaat gtttgccgtc ggcactgtca aacagcagtt
     2881 ggcccagcca gggttcttct tctttattaa taattgcatc cgccgcctgt tccatagcgt
     2941 ttactgcttt ttcaaattca ccgtaacaca tggcattagc agaaaacgac cctatgaaac
     3001 atggaacaaa cgccctggac tgtttattgt gagtgctgct gcaatccgga attccgccgt
     3061 ctttgacgcc ggaacatgga tatttaccag ccgattctat gttatttttc aaaatatttt
     3121 taacggcggt ttttatcctt tcgcgaggat gaaggtcgcc aaggccgaga atatttgccc
     3181 atgtctggcc ggcgaactgg tctattgcag acgtatcgga agaagttcca tttgtcctgt
     3241 tataaaacag attataatat ttgccgttcc acaatttggt ttctattgtg tcggacgctt
     3301 ttttgaatgc gtccgagcac cagttggcga attcttcatc tttgaaagat ttggccattt
     3361 tgattccggc tttgagagca gccagccaca aatcggaaag gtatgaaacc gttccgctca
     3421 tcggccatgt atcccagccc tgatcgaatc catgacagtc cggaatgcca tcattatcaa
     3481 catccagcga cttcccccat tgcattgccc gtttcacttt cggatacatt tttttagcga
     3541 aagccgtatc tgctccaaat acaatattgg tataaactga cagcggaaat gctgcgacat
     3601 cgttggggct gaaaactctg aattctggcg tataaatgct ttcgaaaaaa ccgagtgtcg
     3661 tgggtatttc gccgctttca cgttgcaggg aaacgaaaag ttccagggct tttgcatgaa
     3721 gttcggggaa aaatgctgcc attaatggag ctgcgcattg ttcaagaact atgcaaaaca
     3781 ttctcggaca gtccaatgac tcgtaatagg taaaattacc gttttccatg taccatgtga
     3841 tacgcggcag caggtaaagt gaggaattaa tgaatttcag tgtcttttcc ggcagggaag
     3901 cattggcaat catcggcttc cagctacccg tttttgaccg taacgcttca taatcaggaa
     3961 tggccttaga ggctatctgc tgtgttccgt caggaaaacg tcttgcgtat tcgtggccga
     4021 tataatgacc agtgccgtcc agatgatatg ggaaatacca caacagggcg aaacatatct
     4081 ctttagtttc agccggcgcg agttctattt ctcctgcgac cgctccagcc gggctatgat
     4141 aagaatattt accgagcttg tcatcgaaat tccgctcggc aggaagttcg ccgcgttcaa
     4201 gaaatgaacg ccataatgcg agagctttgt cagtggtgtt tctcaatttt actatttcag
     4261 gattgtcatt catgaagtca ttgcatttaa gaggtatgtt ccaccagtct gcaacggcgg
     4321 aaaattttac gccgtcggtt tttatgccgg tgagcatgta ttccgaattt tttagcaatt
     4381 cttcgtatgt cgacatcaat acagccggat tgtcgtcatt ttcaagcagg gtattgtatt
     4441 tgccgttaga cgccaacaat gggcccagcg gtgcattctt gtgatgcatc cagacggaaa
     4501 tgtcattctg ccatgaaaat gctatcgcag ccttgattgc tgttttgctc gtatttttaa
     4561 ctttgaattt aaagaaaacg agcggtaatg acgatatgtc agcatggtgc ggcaccatcg
     4621 ggctgaaagc ctccatggaa acgtcgcaag gcaattcctt atccgtgtaa tcaatgttca
     4681 gaaatggaaa atctccgcaa taatccagtt tgtcgatgca gggcaggctg ccttcctgcg
     4741 tcagttggag caccttgccg gagctttttt gtggactttc cgtatgaatt gcaaaaaaac
     4801 ttccggcagg cgaaacctgt cgcttggttc tccagttatt ctgcacgcat atgtcctgaa
     4861 aacgtcccgc atggtcaatg gcgagagttc ctgttcccaa tccgcctagc gccattcctg
     4921 tgtgattatt tatttcaccg gagtaatgta tttgcgtctg agccgtcatc atgatggtaa
     4981 ttccttattg catgacaatt tttttgcttc aattcacata cattaatcat agctcaatac
     5041 cataaaatcg tcgatggagg aaattatcat aatgttgtat tatcttatca tatttatccg
     5101 attatgccct tttatgtatt ctctgaaaag acgccccgaa atctgttacg ccagttgata
     5161 atgtgttgca caaggcacat gcatggaaaa agaggtaatg ataatcgtta ttacaatagt
     5221 atagaggaac gtgttccact gcggtttatt atgtgaaact atgagactac agcagaaatg
     5281 agactgtaga acctatccca aaaaccttgg ctgcctgcaa tcggctcttt tgcagcctg
//
LOCUS       DAONFI010000152         5309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18264_length_5309_cov_4.074610, whole genome shotgun sequence.
ACCESSION   DAONFI010000152 DAONFI010000000
VERSION     DAONFI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18264_length_5309_cov_4.074610"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /gene="rho"
                     /locus_tag="PLK08_05990"
     CDS             complement(<1..1058)
                     /gene="rho"
                     /locus_tag="PLK08_05990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPS53084.1"
                     /translation="MPQAAQEETAPQNDQEICIGEGVLEVTDKGYGFLRQAKFNYKPT
                     PGDVFVGKDYVRFRGFKTGLEIKGRITQPNKKKGGPKLEEPIEINGRPAQEYKDIIPF
                     NEQVVVDPHPQLKLETPDGPMEMRVLDIVCPIGKGQRGLLVAPPRSGKTVLLQQIANA
                     ITTNNPECKMMILLVDERPEEVTDFKRKCPKAEIVASCNDMEVRNHTRVAEFTIERAK
                     RMAECGQDVVILLDSITRLGRAYNRSTENSGRTMTGGVDIRALEIPKRQFGAARKIEN
                     GGSLTILATALIETGSRMDDLIFEEFKGTGNMELQLDRKLAERRVWPALDIPKSGTRK
                     EELLVAPWMLPKIHMLRRT"
     gene            complement(2027..4993)
                     /locus_tag="PLK08_05995"
     CDS             complement(2027..4993)
                     /locus_tag="PLK08_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53085.1"
                     /translation="MDSPLIISLHKLLRVAGRKIVLVKLQRQAMLFGIWTSSLAILVC
                     ITGMIVGTLPAWTTWTAWGLWAGILIAFSTNVTITLSRRMNYLRIARLLEQSQPQLGN
                     RLINTLQLDNNESTLARKAVAEAAGGIHSQDILSRLRKAGLTKLVKPALLLAAILPPL
                     LFILLISQNSRQAISMCILPWQTHVTCSTVTTARFDPLKNTAIQLRIIPPDYVGQTPQ
                     TILLGAASQTVQCPAGSVFRFIKNENDNTVTQAISRQAGVLPMTTEFTLDSDTDFHVE
                     ISQPYTRKLLACWPTNGKYSIRILPDNGPQVRITRPAAAATVHPGMKLPVTIISNDDW
                     GLGELRIYLKDAGQKPALIKTVSLAGKRKDTSKITLEFYKYTPPQELSLYADVTNNRP
                     RQPGRAQSNVLHITLESARSISQNQEHMLRNFRQMLQKLLDVQAELGIDADLLTPKAS
                     LASIRKSGVALHAGQTAVRNRLGWLVNFAQKIPSTGELQETVAVLASLNSGPATTAIE
                     QADVIRKLTAAKNVSPAATPLQKTQNEIIDSLQKLLGLLPYATAKMGQKDPQQADALT
                     SSQRKEFRDKLAQQLEQFRVQQQKVVTELGALAAKNVDDFTKADIDAIQKLAAVEDRM
                     EKFMEAAISDLSNLAKQDFGNPSLLEELISVHTDITMAKDALAKKAQEIATLAAQFSI
                     AKGAELEANLEKWLPDKPDRIKWNMEMPSESTDLAQPELPGELEDLMGDLLEQEEDLF
                     EAMDDLTSNNPLSGDKSMGWDAVDGPMTSMNAQGVTGNMLPNTSEISGRSGEGRTGKS
                     SGEYVQQDAVGKGGRRTPTRLGNEAFQAGEINDSSKDSPGGATGGGKISSAGQEGLEG
                     PTPAQLNNRLQALAAKQADLIARSHRISQKYSPGTFANYELKRSVVLMNKVHDELKAG
                     RYKNALQYRQETLDAIRKTHDLTADPMKIARDASKELPKKIQDDIADSAGGKLPPDYS
                     EILQKYFQRLAETTEK"
     gene            5182..5271
                     /locus_tag="PLK08_06000"
     tRNA            5182..5271
                     /locus_tag="PLK08_06000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5216..5218,aa:Ser,seq:gct)
BASE COUNT         1134 a         1272 c         1350 g         1553 t
ORIGIN      
        1 gttctgcgaa gcatgtgtat tttaggcagc atccacggtg cgacgaggag ttcttctttt
       61 cgcgtgccgc ttttgggtat atccaatgcc ggccacacac gtctttccgc cagcttgcgg
      121 tcgagctgaa gttccatgtt gcccgtgccc ttgaactctt caaagatgag gtcgtccata
      181 cggctgccgg tttcaatcag ggctgtcgca agaatcgtaa ggctgccgcc gttttctatt
      241 tttctggctg cgccgaactg ccgcttggga atttccaacg cccttatgtc cacgccgccg
      301 gtcattgttc tgccggaatt ttccgtggac ctgttgtacg ctctgccgag gcgggtaatg
      361 ctgtccagaa gtatgacgac gtcctggccg cattcggcca ttcttttcgc acgttctatc
      421 gtaaattcag ccacccgcgt atgattgcgc acttccatat cgttgcatga cgcgacgatt
      481 tcggcttttg ggcatttacg cttgaaatcg gtgacttctt ccggacgttc gtcgaccaga
      541 agaatcatca tcttgcattc aggattattg gtcgtgatgg cgttcgcaat ctgttgcagc
      601 aatacggtct tgccagacct cggcggagcg acaagcaggc cgcgctgacc tttgcctata
      661 gggcatacga tatcgagaac acgcatctcc atggggccgt cgggagtttc cagcttgagt
      721 tgcggatggg ggtcaaccac gacctgttca ttgaacggaa taatgtcctt atattcctgg
      781 gctggccggc cgttgatttc tatcggctct tccagcttcg ggccgccttt tttcttgttg
      841 ggctgagtta ttctaccctt tatctccaag ccggtcttga atcccctgaa tcgaacgtaa
      901 tccttgccta cgaatacgtc gcccggagtg ggtttgtaat tgaacttggc ctgacgcaga
      961 aacccgtaac ctttgtctgt aacttccaga acgccttcac ctatgcagat ttcctggtcg
     1021 ttttgcggtg cggtttcttc ctgagctgct tgtggcataa actttttcct caattaataa
     1081 taatcaaata gaactatccg tcaaaaaatg taaaagcatt tttcatgcat ctttcgtaat
     1141 tttacgaatt cggtcaggca gataaagtga ctgctgaata acctctcatg ccgtaattaa
     1201 atgcgcgact gttattggtt tagaatgctc taagcctgca aaccataaaa atttctaccg
     1261 ttcacacgaa ccgcaggcat acaccagaac agtggtcgga tagatgagct taatccgata
     1321 attgtttcga ttccggcatc catgccttat tataaatgcg tctcacaaaa tgccgccgca
     1381 ttaagtcaaa cattgcctcg atactacgtt tcattttgtt agaagctcta aaataaaatt
     1441 cacacttttt aaaaactact cggtttaaga gcatgaattt cgtaacccgt aaagtcaaat
     1501 cttacgtact ctgaagggat acgatattac tcgtcagttt ttgagccgac tttgactggg
     1561 ctgataaatt ccctgcacaa taaatgtggt ggatggaaaa caactcagac aatcttccgt
     1621 tcagaacttt aaatccactc aagctggatt ttaagcattt gtgacaaaaa tctatcgttt
     1681 cagttgatta ccttcattca tatctgcggc gaaaaaacgg taaatgaatc tcgtcagaga
     1741 tactgccgcc ttgtttattg ttcagaatta aatcactttt gacaaaagac gacatcattt
     1801 ttgtaaaatg ctagcggctt ccgcgagact tccgagcaaa caaaaaaacc aaactttggc
     1861 tttgactgtg aaaacggagc agtctcagag ttaccctcaa caacttactc ttatactcaa
     1921 aaaaaaggct tttgtctacc ccttttttaa aaaaacttta aatttgtctg catgaagatt
     1981 tttggaattt ttggattttt tgaaaatttt tgaaaatttt tgaaaattat ttctcagtag
     2041 tttccgccag tcgctgaaaa tatttctgca gaatttcgga atagtccgga ggaagcttgc
     2101 cgccggcgga gtctgcaatg tcgtcctgga ttttttttgg cagttccttt gatgcatccc
     2161 ttgcaatttt catggggtct gccgtcaggt cgtgcgtttt ccttattgcg tccagtgttt
     2221 cctgcctgta ttggagggca tttttatagc ggccggcttt gagttcgtcg tgtactttgt
     2281 tcatgagcac tacggagcgt tttagttcat aattcgcaaa agtgccgggc gaatattttt
     2341 ggctgatgcg gtgcgacctg gcaattaagt ccgcctgttt tgccgccaat gcctgcaggc
     2401 ggttgttgag ttgtgccggc gtagggcctt cgaggccttc ctgacctgcc gatgaaattt
     2461 ttccgccgcc tgttgctcct cctggtgaat cttttgacga gtcgtttatt tcgccagcct
     2521 gaaatgcttc gttgcccagc cgtgtcggcg ttcgtcggcc gcctttgccg actgcgtcct
     2581 gctggacgta ttcgccgctg ctcttgccgg tgcgtccttc gccgcttctg ccggaaattt
     2641 cgctggtatt gggcagcatg tttccggtca cgccctgagc gttcatactc gtcatcggcc
     2701 cgtcaaccgc atcccaaccc atggacttgt cgccggagag cgggttgttc gacgtcaaat
     2761 catccatagc ttcaaacagg tcttcttcct gttcgagcag gtcgcccatg aggtcttcga
     2821 gttctcctgg cagttccggc tgggcaaggt cggtcgattc cgacggcatt tccatgttcc
     2881 attttatcct gtcgggcttg tccggcagcc atttttccag gttcgcctca agctctgcgc
     2941 cttttgcgat actgaattgt gcggcgagag tggcgatttc ctgggccttt tttgccagag
     3001 cgtccttggc catggttatg tcggtgtgaa cggatatcaa ttcctcaagc agcgacggat
     3061 tgccgaaatc ctgttttgcc agatttgaca ggtcgctgat ggcggcttcc atgaattttt
     3121 ccatccggtc ttcaactgcc gcgagtttct gtatagcgtc gatatccgct tttgtgaaat
     3181 catcgacgtt tttggccgcc agtgcgccga gctcggttac gactttttgt tgctgcacgc
     3241 ggaactgttc aagctgttgt gcaagtttat cgcgaaattc cttacgttgg ctgctggtaa
     3301 gagcgtctgc ctgctgcggg tctttttgcc ccatcttcgc cgtcgcgtaa ggaagcaggc
     3361 cgaggagttt ctgaagcgag tcgattatct cgttttgtgt cttttggaga ggcgtcgcgg
     3421 caggggatac atttttggct gccgttaatt ttcgtattac atccgcctgc tcgatggctg
     3481 tcgtcgccgg cccgctgtta agagatgcaa gaacggcaac cgtctcctgc aattctccgg
     3541 tcgacgggat tttttgtgca aaattcacca gccagccgag tctgttacgc acggcagttt
     3601 ggccggcgtg caatgcaact ccggattttc gaatggatgc gaggctggcc tttggagtaa
     3661 gcaggtctgc gtctataccc aattcagcct gaacgtccag cagtttttgg agcatctgcc
     3721 ggaaatttcg taacatgtgc tcctgatttt gcgatatgga cctggctgat tctagcgtta
     3781 tatgcaagac gttgctttgg gcgcggccag gttggcgagg tcgattattg gtgacgtcgg
     3841 catatagcga taattcctgc ggcggcgtat atttatagaa ttcaagcgtg attttcgacg
     3901 tgtccttgcg tttgccggct agagagacgg tttttatcag cgccggtttc tggcctgcgt
     3961 ccttgagata aatccgcaac tcgccaagtc cccagtcgtc gtttgagatt attgttacgg
     4021 gtagtttcat gcctggatgg actgttgctg cggcggcagg tctggttatg cgaacctgcg
     4081 ggccgttgtc cggcaggatt cgtattgaat atttgccgtt tgtcggccag cacgccagga
     4141 gttttcgtgt ataaggctgg gaaatttcca catggaaatc ggtatcgctg tccagtgtga
     4201 attcagtagt catcggcaga acgccggctt gccgcgaaat cgcctgcgtg acggtgttgt
     4261 cgttttcatt tttgatgaag cggaaaaccg agccggctgg gcattggacg gtttggctgg
     4321 ctgcgccgag caaaatggtt tgaggcgtct gaccgacgta atcgggggga attatacgca
     4381 actgaatggc ggtgtttttg agagggtcga atctggcagt agtgacggtt gagcatgtta
     4441 cgtgcgtctg ccagggaaga atacacatgg aaatcgcctg gcgggaattt tgggaaatga
     4501 gcaatatgaa caatagcggc ggcagaattg cggctagcag cagggctggt ttaaccagtt
     4561 tcgtcaagcc ggctttgcgc aaacggctga ggatgtcttg agaatgtatg ccgccagccg
     4621 cttcagcgac ggctttcctg gcaagggtcg attcattgtt gtcgagttgc agcgtgttta
     4681 taagccggtt gccgagttgc ggctgacttt gttcgagcag ccgtgcgatt cgaagataat
     4741 tcatccgcct ggaaagggtt attgttacgt ttgttgagaa tgcaataaga atgccagccc
     4801 acaaccccca agccgtccat gtcgtccaag ccggcaaagt gccgactatc attcctgtta
     4861 tgcataccag tattgccagc gacgaggtcc atatgccaaa aagcatcgcc tgcctctgta
     4921 gcttgacgag gactattttt ctgccggcta cgcgcagaag cttatgaaga ctgatgatta
     4981 gcggtgaatc cattcctgaa gtataacaga taactctgcc gccgtgaagc cgtagaattc
     5041 gaagttattt ttttaaaaag cgatataaga ccttgaccgg cgaagtgaaa gacaatacat
     5101 tatctctccc ttgccgtact atcaggtggc aagttttttt aaggcttgaa ttcgcttgat
     5161 attacttgtt tagcgatgat tggagaggtg gcagagaggc tgaatgcacc ggtttgctaa
     5221 accggcatac ggtttaacgc cgtatcgcgg gttcgaatcc cgccctctcc ggtagattcg
     5281 gcggtattgt aagaatatca gccaaagtc
//
LOCUS       DAONFI010000153         5291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18356_length_5291_cov_3.821429, whole genome shotgun sequence.
ACCESSION   DAONFI010000153 DAONFI010000000
VERSION     DAONFI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18356_length_5291_cov_3.821429"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="PLK08_06005"
     CDS             <1..1717
                     /locus_tag="PLK08_06005"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HPS53086.1"
                     /translation="RGAGYAGGSGACPRCKTPLKIPRPLAADSPNYVTGQRYLALPLT
                     AAPADEIPTVVTDDMGPSRKFKCPDCGQLFESLPFEPHNPAQCPACSKTGTPLGQPTE
                     FPRAKSDLQTKNSPPQQNVNESQIKPNDIVDGYLVLQPAESQEADTVIEGIAVDDNGN
                     EINDQPAVENAQQKIPQDIPPQKTSKAQSDAKPQPEESQITSEKRISHPLSRSRTQTN
                     ALKTPPNIKNIPEGKWIYFIKGKVTGPLDTQSIRNMLAKGELSQSVLVWKSGMDNWLP
                     ADTFDELRIENQVAVPEKISFQDDSSTASAKTLNSRLQFLLWLAVILWAIVILTFLTR
                     KTLKDIGIEAYTACVVVLLSFSAAGLGLGVMLIIKYGRSFLKSGIKSCIEWLSAMVAF
                     ICAIAISFSVYTHVDTTPVFENVEQYNKIAMDGFNSLYAGNFNDTLNRVNWETFVFNK
                     RNVGQEFKSAATQKQKMLIIADVMDKLRKQVDTASVYGSRTLRTAQSQPAGSQPSTQP
                     QKTAATDPQIPVLLIYSWTVTKQNADGTEVRGYCRNGCRINITIRGGMITEISIRQPE
                     ESKTE"
     gene            1800..2534
                     /gene="tsaB"
                     /locus_tag="PLK08_06010"
     CDS             1800..2534
                     /gene="tsaB"
                     /locus_tag="PLK08_06010"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPS53087.1"
                     /translation="MKHDGKISVAIETSCRLGAVALGIGDKMISSRMLGTSGRHAAEL
                     VACLDEMLREAAIKPGDVNELYVSVGPGSFTGLRVGITVARTWSQLTEGLRLFAVPTS
                     LAVAYNYVNDDWRQMCVMLAAKEHTVHATLLTKDNGRISIIGKPVLLDPITAIETMPR
                     PLTVAGEALGFCGETLKKLSAADGITIAAESHWLPEIFSLWHAGNELAATDGCLTDFE
                     HLLPEYARRPEAVRLWEHKHLQLNGF"
     gene            2557..3213
                     /locus_tag="PLK08_06015"
     CDS             2557..3213
                     /locus_tag="PLK08_06015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPS53088.1"
                     /translation="MKIMQCWDDGVSNDERLTELLRKYNAKATFNIIPGLHGKSRIFT
                     SVYGNTFKSYRLALDEMKDIYKGFKVAGHSMTHPKLAELDAQTLEREIVDCKDFIQNF
                     FGQQECGFAYPYGNYNDAVKQALANAGYRYARTVKNINGALPLDDPMELPSHCHFLNP
                     DFWKKYEEVRAIDGIFYFWGHSYEMMDDPKMWDEFESKLDRIKSDSKAQWIDIIDLFV
                     "
     gene            complement(3442..>5291)
                     /locus_tag="PLK08_06020"
     CDS             complement(3442..>5291)
                     /locus_tag="PLK08_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPS53089.1"
                     /translation="STAGICMGTSLAFPAIVCAGHSKEAEQWFEFPLALYAYRFLPSM
                     GFDGDNNEGMAYWTFGNSFIMKYADLMREICGIDLYKHPWLTKTARFPVYCAPPNSFM
                     VSFADYTQNGLPNHGNKGPVSRKLVGNLGRRTEDSYALWYGGVPCFKTIKAKAPVDIP
                     QSMHYRYIGWAVFNTCLTDSRENVALGFHSGKFLAAHQHGDQNSFVINAYGDKLAVDG
                     GYYDWYDSPHFNDYSTQTKAHNTILVDGQGQAVRRFGADGKISEYYDSSSWGYTEGDA
                     SNPRIYGGGVLTEFKRKMIFMKPSFIISYDSILSRKPAVFSWLLHAQSDSPIKIEQQP
                     ETFQINRPLAWLRGKVIFPEKAGMKISSGYDVLPYMPLSSKPMKMEDIEKEWTLNIYN
                     RQKEKSVEYLVGFQVGKSDDNQNKYADFVGLENKFGLGVIVSQPDSYNYVVIYNRNPG
                     QKMEPLYNLATDGNIAAIKFGEGEKIIDALLIGGTFLSYKDKDIYRSDSVGNWSMIKP
                     EQVLVQHENNKIVRINGESIPCDVYKSRQADGKYLTVITGIMKVEKQTSYNVSSACSS
                     LNYRICNPDAEFTGNVGKSKPHESILLPAGEYLITFTSIYSIDNISMDS"
BASE COUNT         1496 a         1359 c         1154 g         1282 t
ORIGIN      
        1 ccgcggggcc gggtacgccg gcggcagcgg cgcatgcccc aggtgcaaaa cgccgttaaa
       61 aatacccagg ccgcttgcgg cagactcgcc taattacgtc accggccagc gttatctggc
      121 gttgccgctc acagccgcac cagccgacga aattccgacg gtcgtaaccg acgacatggg
      181 gccgagccgg aaattcaagt gtcccgactg cggccagctt tttgaaagtc tgccgtttga
      241 gcctcataat cccgcccaat gtccggcctg ttcaaaaacc ggtacgccgc tcggccagcc
      301 gacggaattc cccagagcca aatcagactt gcaaacaaaa aatagccctc cacaacaaaa
      361 tgtcaatgaa tcgcagataa aaccgaacga tatcgtggac ggttacctcg tactccagcc
      421 agccgagtcg caggaagcgg ataccgtcat cgaaggcatc gcagtcgacg acaacggcaa
      481 tgaaataaac gaccagccag ccgtcgaaaa tgctcaacag aaaattcctc aggatattcc
      541 gccccaaaaa acgtcgaagg ctcaatccga cgccaaaccg caacctgaag aatcgcaaat
      601 aacttcggaa aaacgcatct cgcacccact ttcacgcagc cgcacacaga caaatgcatt
      661 aaaaacaccg ccaaacataa aaaacatccc ggaaggcaaa tggatttatt tcataaaagg
      721 taaggtcaca ggcccgctgg atacgcagtc gatacggaac atgctcgcaa aaggagaact
      781 cagccagtca gtactggtgt ggaaatccgg catggataac tggctgccgg ctgacacttt
      841 cgacgaactc aggattgaaa atcaggttgc cgtccccgaa aagatatcat ttcaagatga
      901 ctcgtcgacg gcatctgcaa aaacgctcaa ttcgcggctt caattcctct tgtggttagc
      961 cgtaattttg tgggcaattg tcatattgac gttcctgacc cgcaagacgc tcaaagatat
     1021 aggcatagag gcgtacactg cctgcgtggt agtgctgttg tccttctccg ccgccggact
     1081 gggcctgggg gtgatgctga taataaagta tggccgctct tttttaaaat ccggcatcaa
     1141 aagttgtatc gaatggcttt cagcgatggt ggcattcata tgcgcaatag ctatttcttt
     1201 ttccgtctat acgcatgttg atacaacacc ggtttttgaa aatgtggagc aatacaacaa
     1261 aatagccatg gacggtttca acagcctcta cgccggcaat ttcaatgaca ccctcaacag
     1321 ggtcaactgg gaaacattcg tattcaacaa gagaaacgtc ggccaagaat tcaaatccgc
     1381 cgcgactcag aaacagaaaa tgctcataat cgccgacgtc atggataaat tacgcaagca
     1441 ggtggacacg gcatccgttt acgggtcgcg aacccttcgg acagcacagt cacaacccgc
     1501 cggcagtcag ccctcgacgc aaccacaaaa aacagccgcc acagacccac aaatacccgt
     1561 tcttctcata tatagctgga cagtcacgaa acagaatgct gacggcacgg aagtaagagg
     1621 ttactgccgc aacggctgtc gaatcaacat cacgattcgc ggcggcatga taacggaaat
     1681 ttcgatacgt cagccggaag agtcaaagac ggaataaaca cgtcaaacct gcatgccagg
     1741 tcatacacct catagatttg atttttacgg ttcaaactgt agaatacgca aaaagacata
     1801 tgaaacatga cggaaaaata tcggtcgcaa tcgaaacaag ctgccgcctg ggtgccgtag
     1861 cactgggtat cggcgataag atgatttcat cgcgcatgct cggtacttcc ggcaggcatg
     1921 ccgccgaact ggtcgcatgt ctggatgaaa tgctgcggga ggcggcaatc aagccaggcg
     1981 atgtaaacga actttacgta tccgtaggac ctggcagttt caccggcctg cgggtaggca
     2041 taacggtcgc gcgtacgtgg tcgcaactca ctgagggatt gaggctcttt gccgtaccga
     2101 cgtcgctcgc cgtggcatat aattacgtca acgacgactg gcggcaaatg tgcgtaatgc
     2161 tggcggcaaa ggaacacacc gtccacgcaa cactgctgac gaaagacaac ggccgaattt
     2221 ccatcatcgg taagccggta ttgctagacc cgataacggc gattgaaaca atgccgcggc
     2281 cactgacggt cgccggcgaa gctctcggct tctgcgggga aacgctcaaa aaattatccg
     2341 ctgccgacgg cataaccatc gcagccgaat cgcattggct gccggaaata ttcagcctct
     2401 ggcatgccgg caatgagctt gcggcaaccg acggctgtct gacggatttc gaacacctgc
     2461 ttccggaata cgcacgccgc cctgaagcgg taagactctg ggaacataaa catcttcaat
     2521 taaacggatt ttaatttctt tttttaggat tttccgatga aaatcatgca atgctgggat
     2581 gacggcgtct ccaatgacga acgcctgact gagcttttga gaaaatacaa tgccaaggcg
     2641 acgtttaaca taattcccgg cttacacggc aaaagccgaa tattcacgtc agtttacgga
     2701 aatacattca agagctaccg tctcgcgctg gacgaaatga aagacattta caagggattc
     2761 aaagtagccg gccattccat gacccatccc aaattggctg aactggacgc ccaaacgctt
     2821 gaaagagaaa ttgtcgactg caaggatttc atccaaaact tcttcggcca acaagagtgc
     2881 gggtttgcat atccatacgg caattataat gacgccgtca agcaggctct cgcaaatgcc
     2941 ggatatcgct acgccagaac ggttaaaaat atcaacggcg ccctgccgct tgatgacccg
     3001 atggaactgc cttcacactg tcacttcctc aatccggatt tttggaagaa atatgaagaa
     3061 gttcgtgcca tcgacggaat attctacttc tggggccatt cgtacgaaat gatggatgac
     3121 cctaaaatgt gggatgaatt cgagtcaaaa ctcgacagga taaagtccga ttctaaagcg
     3181 caatggatag acatcataga cctgttcgtc taacaaaacg ccttacaaac gaaatacgta
     3241 gtacggagta cctgaataaa atatacatag aacctatccc aaaaaccttg gctgcctgca
     3301 atcggcactt ttgcaacctg gcggcgtcag aaaaacggca gaatatcctt aaatatgccg
     3361 ccatttttct tccttgccag cccgcaaaat tgctcggccc atgtttttgg gataggttct
     3421 aataaaagcg ttttataaaa ttcaggaatc catactaatg ttatctatcg aataaatact
     3481 cgtaaacgta ataagatatt ctccagctgg caggagtata ctttcgtgcg gttttgactt
     3541 gccaacgttt cctgtaaatt ccgcatcggg attgcaaatt ctataattca atgaactgca
     3601 agccgacgag acattatatg acgtttgttt ttcaaccttc attatgccag ttatgaccgt
     3661 aagatattta ccgtcagcct gccggctttt ataaacatcg caaggtatgc tttcaccatt
     3721 gattctgact atcttattgt tttcatgctg cactaaaacc tgttccggtt ttatcatact
     3781 ccagttccca acggaatctg aacgataaat atccttatcc ttgtaagaaa ggaaagtccc
     3841 gcctataaga agcgcatcga ttattttctc tccttcgcca aacttgattg cagcgatatt
     3901 cccatccgtc gccaggttat ataaaggctc catcttctgc cccggattcc tgttataaat
     3961 gaccacataa ttgtatgaat ccggttggct gactattact cccaaaccaa atttgttttc
     4021 cagtccgaca aaatcggcat atttgttctg attgtcatcg ctcttgccaa cctgaaatcc
     4081 aaccagatat tcaaccgatt tttctttctg tcgattatat atgttaagtg tccattcctt
     4141 ttcaatatct tccatcttca tcggcttgga cgacaaaggc atatatggca aaacatcata
     4201 gccggatgaa attttcattc ctgccttttc aggaaaaatc actttcccgc gcagccatgc
     4261 caatggccga tttatctgaa aagtttccgg ctgctgttca atttttattg gcgagtcact
     4321 ttgtgcatgc aacagccatg agaatacagc cggctttcgt gataatattg aatcatatga
     4381 aataatgaaa gacggtttca tgaaaatcat tttccgcttg aattcagtca acactcctcc
     4441 gccgtaaatt cgtggattgg aggcatcgcc ttccgtatat ccccatgagc ttgagtcgta
     4501 atattccgat atttttccat ccgccccgaa tcggcgtacg gcttgtccct gtccatctac
     4561 gagaatagta ttatgcgctt tcgtctgagt tgaataatcg ttgaagtgcg gactgtcata
     4621 ccaatcataa taacctccgt caacagcgag tttatcgcca tacgcattta ttacgaaaga
     4681 attctggtct ccatgctgat gtgccgcaag aaatttcccg ctgtgaaagc ctaatgccac
     4741 attttcccga ctgtccgtca gacacgtatt gaatacagcc caaccgatat atcgatagtg
     4801 catggattgc gggatatcca ctggagcctt tgccttgata gttttaaaac aaggcacgcc
     4861 gccataccat aaggcgtatg aatcttctgt acgccttccg agattgccga caagttttct
     4921 gctgacagga cctttgttgc cgtgatttgg caatccattc tgcgtataat cggcaaaaga
     4981 gaccataaag ctgttcggtg gagcacagta tacgggaaat cgagctgtct tcgtcagcca
     5041 aggatgttta tataaatcta ttccacagat ttctctcatc aggtcagcat atttcattat
     5101 aaaactgttg ccaaaggtcc aataagccat cccttcatta ttatcaccat cgaatcccat
     5161 cgacggcagg aatctgtatg catataatgc caacggaaat tcaaaccatt gttcggcttc
     5221 tttggaatgt ccggcacata caattgcagg aaaagccaaa ctcgttccca tacagattcc
     5281 tgccgtcgat g
//
LOCUS       DAONFI010000154         5285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18394_length_5285_cov_3.675335, whole genome shotgun sequence.
ACCESSION   DAONFI010000154 DAONFI010000000
VERSION     DAONFI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18394_length_5285_cov_3.675335"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLK08_06025"
     CDS             <1..753
                     /locus_tag="PLK08_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53090.1"
                     /translation="FMIDDKIIPFFAGDVSVITEYEFHIAQSADSPSLWTFISLNPTE
                     LLYPCKSIGIEHFSGWKFPNILTQHKHGGIIALVREIISELEFKQPNYQDAVKGIAMA
                     IMCKLHRIGSKVNRNDNLHVNKIFERIAPAINYIHANYMKAIRISKLAKFCHLSEPHF
                     RRLFVSATGKSPQEYLTHLRISLAGILLKDGDRQILDIALLVGYPSLSGFNRHFRRIM
                     DCSPRNWRNLEKLHLTTDITHHHKSIIPQNDY"
     gene            complement(1040..2461)
                     /locus_tag="PLK08_06030"
     CDS             complement(1040..2461)
                     /locus_tag="PLK08_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPS53091.1"
                     /translation="MTAYNPVTPQIAHELEEIVGSRYVIYNDPEKLENYSHDEIAEAQ
                     YAHMPEVVVRPDSAQQISEIMKLANLRNVPVTPRGAGSGLSGGAVPIYGGIVLHVDRM
                     NHVLEIDPKNMMITVEPGVVTNEINDLIKPYGLFYAGYPMSLETCYIGGNVAENAGGG
                     KAVKYGVTGRYVTGLEVVMPTGEIVHFGGKLLKDVTGYNMVQLMVGSEGTLGIFTKIT
                     LKLMPLPKATIDLLCLFDTAQQAISAVPEIMTSGGIIPTAIEFMDRTSIKASCDYLNE
                     TIPYEKCGAMLLITVDASCEQQAIMESDAIGELCEKCGATEVYIADNHTTSERIWKVR
                     RNIAEAFKVYSPHQSLEDIVVPIANIPDMVSRLEVLAKKYDCLIPCYGHAGDGNLHAT
                     PVMNPAWTAEKWHKLLPEILTEIYRITGELGGMISGEHGIGHKRKEYMILTTSQEYIN
                     ILRSIKKSLDPRCILNPGKIFDV"
     gene            complement(2458..3474)
                     /locus_tag="PLK08_06035"
     CDS             complement(2458..3474)
                     /locus_tag="PLK08_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPS53092.1"
                     /translation="MHKNDDYKGVWILAEQSAGRVLRVSYELLTRGRELADKRKTDLT
                     AIIFGAAFDDCDLQQLIECGADSIIAVEAKQLEHFDVEPYSACMLKLIAEKKPEIIIA
                     AATTTGRTLMPYVAIKADTGLTADCTVLDIEPQTGNLLQTRPAIGGNIMATIKTPNHR
                     PQMATVRPRSTKPAERKADRTGQITRINASSDTLTTRAKFLGFNPDESEHGLQDADII
                     VAAGRGVKKATGVETVKTLCEKLDGALGASRDVVDRGWLSYPHQIGLSGKTVSPKLYV
                     ACGISGAIQHLAGMQTSENIVAINTDPDAQIFRVADLGIVGNIFEVLPVICEKLDALK
                     RGEK"
     gene            complement(3483..4280)
                     /locus_tag="PLK08_06040"
     CDS             complement(3483..4280)
                     /locus_tag="PLK08_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPS53093.1"
                     /translation="MKIILPIKQVPETGNVRMDEQTGTMVRDGVESIVNPLDLYAIEL
                     ALRLREQNGGEIIAITMGPPSAEKALREAVAMGCDSAILLSDKSFAGADTWATGYVLS
                     QAIRNIGGYDMIICGERATDGDTGQVGPGIASFLDLPVMTYVCAVDEISDKSCRVKRL
                     IEDGNEKWEVNLPTVLTVVKEIAVPRLPTLRSKQKSRALKIPAWGAADVNADVNLTGL
                     AGSPTRVVKIFRPQVSRKCEKISATDETTINAAADLFVEFLAKRELV"
     gene            4364..5089
                     /locus_tag="PLK08_06045"
     CDS             4364..5089
                     /locus_tag="PLK08_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPS53094.1"
                     /translation="MSTEISNSAYGYIRSRLLSGRYAPGVRVSEFSVAKELGISRSPV
                     REAISHLISEGLLQKLPGYGAYVKIPDRREIEELMVVRELMEEYVVTKAASWSDPNQI
                     EELQKICDELHGLACQARHNDIEDKIIDRLIELDFAFHTRLYELADNRQIIQIMKNAR
                     ILSAILSTYQKDFFNLSEIAHIWREHTRILQSIRRRNPQNARYWLKCQIRRSRQLYKS
                     GYEKLMENPRFRELRELSKVAKS"
BASE COUNT         1263 a         1263 c         1236 g         1523 t
ORIGIN      
        1 ttcatgatag acgacaaaat aatacccttt ttcgccggcg atgtttccgt gattaccgaa
       61 tatgaatttc atatcgccca gagtgccgac tctccaagcc tctggacatt catatcgctc
      121 aatccaacgg aactacttta tccgtgcaaa tctatcggaa ttgaacactt ctcaggctgg
      181 aaatttccaa acatactgac gcagcataag catggcggca taattgcgtt ggtacgcgaa
      241 ataatatcag aactggaatt taagcaaccc aactatcaag acgccgttaa aggaatagcg
      301 atggcgataa tgtgtaaact tcatcgaata ggttcaaagg taaaccgcaa tgataatttg
      361 cacgtcaata aaatctttga aagaatcgcc ccagccatca attacattca tgccaactac
      421 atgaaagcaa tcaggatttc aaaactggcg aagttctgtc atctcagcga accccatttt
      481 cggaggcttt tcgtttcagc caccggtaaa agtccacagg aatacctgac tcaccttcgt
      541 ataagtcttg ctggtatcct tttaaaagat ggcgacaggc agattctcga cattgcatta
      601 ttggttggct atcccagcct ttccgggttc aaccggcatt tcaggcgaat catggattgc
      661 tcgccacgca attggcgcaa tttagaaaaa ttacatttaa ccaccgacat tacccaccat
      721 cataaatcaa ttataccgca aaacgactat tgaaaattat tcttatttgg ttctgcctca
      781 attggtatta atgcgttatc ttcaattgcc aaaacattaa cgcttccttc agccacgaac
      841 tgttcggcga gccatgactt caactgctgt attctcgtta ttttgcactg ttttaagttc
      901 atcagtacgc gtcattcgcc agcaatatga tattgagcat atttatgata atttttgcag
      961 cagttttcgg caaatttctg gatatgatag gtcagatgtt ttcgcgttaa acgcctttca
     1021 gggaaacgat gcacggtact cagacgtcaa aaattttacc ggggttaaga atgcatcgcg
     1081 ggtcgagcga tttcttgatt gaccgtagaa tattgatgta ttcctgagat gttgtaagta
     1141 tcatatattc cttgcgcttg tggccgatgc cgtgttcgcc tgatatcata ccgccaagtt
     1201 caccggttat gcgatatatc tcggttagaa tttccggcag taatttatgc catttttctg
     1261 cagtccaggc tggattcatt accggcgttg cgtgcaggtt gccgtcgccg gcgtggccgt
     1321 agcacggaat caggcaatcg tattttttcg ccaacacttc cagccggctg accatgtcgg
     1381 gaatgttggc tattggcacg acgatatctt ccaggctctg atgcggcgag taaactttaa
     1441 atgcttccgc gatgttgcgt cgtactttcc agatacgctc ggacgttgtg tgattgtcgg
     1501 caatatagac ttccgtcgcg ccgcattttt cgcaaagttc accgatggcg tcgctttcca
     1561 tgattgcctg ttgttcgcat gatgcatcta ctgttatcag cagcattgcg ccgcattttt
     1621 catatggtat ggtttcattg aggtaatcgc aggatgcctt tatgcttgta cggtccatga
     1681 actcaatggc tgtcgggatt atgccgccgg atgtcattat ttccggcacg gcggatatcg
     1741 cctgctgtgc ggtatcaaaa aggcagagca ggtctattgt cgctttaggc agcggcatca
     1801 gttttaatgt tatttttgtg aaaatcccca gagttccttc tgagccgacc atgagttgaa
     1861 ccatgttgta accggtgacg tccttgagca gtttgccgcc gaagtgtact atttctccag
     1921 tgggcatgac aacttccagg ccggttacat atctgcctgt cacgccgtat ttgacagctt
     1981 ttcctccgcc ggcgttttct gccacattgc cgccgatata acacgtttcc aggctcattg
     2041 gatagcccgc gtaaaacagg ccgtagggtt tgataaggtc gtttatctcg tttgttacca
     2101 cgccgggttc gacggttatc atcatatttt tcgggtcaat ttccagtacg tggttcatcc
     2161 tgtcgacgtg cagaactatg ccgccgtata tcggcacagc tccgcctgag agcccgctgc
     2221 ctgcgccacg cggagttaca ggcacgttgc gaagattagc cagcttcatt atttccgata
     2281 tctgctgagc cgaatctggc cgtaccacga cttccggcat gtgggcgtat tgagcttcgg
     2341 ctatttcgtc atgagaataa ttttccagct tttcggggtc attgtatata acgtaccgcg
     2401 agccgactat ttcttccagt tcgtgtgcga tttgcggcgt taccggatta tatgctgtca
     2461 tttttcgcct cttttcaatg catcaagttt ttcgcatatg accggcagca cctcgaatat
     2521 attgccgact atgcccaaat cagccaccct gaaaatctgt gcgtcaggat cggtgtttat
     2581 cgccacgatg ttttccgacg tctgcatgcc ggcgaggtgt tgaatggcgc ctgatatgcc
     2641 gcatgcaacg tatagtttgg gacttaccgt cttgccgctg aggcctatct gatgcggata
     2701 actcagccag cctctgtcta ctacatcgcg cgatgcgccg agtgcaccat ccaatttttc
     2761 acataatgtt tttaccgttt ctacgccggt tgctttcttt acgcctctgc cggctgcaac
     2821 gataatatcg gcatcctgca agccatgttc ggattcgtcg gggttgaatc ccagaaattt
     2881 ggcgcgtgtg gttagtgtgt cagatgacgc atttatccga gtgatttggc cggttctgtc
     2941 ggctttccgc tctgcgggtt ttgttgaacg tggccgaacc gtcgccatct gtggccgatg
     3001 atttggcgtt tttatggtcg ccattatatt cccgccgatt gcagggcggg tttggagcag
     3061 gttgccggtt tgcggttcta tatccagaac agtgcagtcg gcggtcaggc cggtgtcggc
     3121 tttgatggca acatacggca taagcgtacg gccggtagtc gtggcggctg ctattatgat
     3181 ttcaggtttc ttttccgcga taagtttgag catgcacgcc gagtacggtt cgacgtcaaa
     3241 atgttccaac tgctttgcct ctactgcaat tatgctgtca gccccgcatt cgatgagttg
     3301 ctgcaaatcg cagtcatcaa acgccgctcc gaatatgatt gccgttaaat cggtcttgcg
     3361 tttatccgcc aattctctgc cgcgagtaag caattcataa cttacccgca aaacacgtcc
     3421 tgccgactgt tccgcaagaa tccatacgcc tttataatca tcgtttttat gcatgttttg
     3481 cctcagacca gttcacgctt tgcaagaaat tccacaaata agtccgctgc cgcattaatt
     3541 gtcgtctcgt ccgttgctga aatcttttca catttgcgtg aaacctgggg ccggaagatt
     3601 tttacgactc ttgtcggcga gccggccaga cctgtgagat tgacatcggc attgacgtcg
     3661 gctgctcccc acgccggaat tttcagagcc cgtgatttct gtttgcttcg gagcgttggc
     3721 agacgcggaa cggcgatttc cttgacaaca gtaagcacgg ttggcagatt tacttcccat
     3781 ttttcattgc catcctctat caggcgtttt acccgacagc ttttatcgga aatttcatcc
     3841 actgcacaga catatgtcat caccggcagg tcgaggaatg acgcaatacc agggccgacc
     3901 tggccggtat cgccatccgt ggcacgttcg ccgcagatta tcatatcata tccgccgatg
     3961 ttacgaatag cctgtgaaag cacgtaacct gttgcccatg tgtcggcgcc tgcgaaagat
     4021 ttgtcggaaa gaaggatggc agaatcgcag cccatagcga cggcttcacg cagtgccttt
     4081 tccgccgatg gcggccccat ggtaatggcg attatttcgc cgccgttctg ttcacgaagc
     4141 cgtaacgcaa gttcaatggc atacaggtca agcggattaa ctatggactc gacgccatcg
     4201 cgaaccattg tgccggtctg ttcatccatc cggacattgc ccgtttccgg tacctgcttt
     4261 ataggaagga ttattttcat atcgactcca aaattgcata aaactgtata ttatttgcag
     4321 tttatgcttg acttttttta taaaatatga aaactgtcga catatgtcaa cagaaatttc
     4381 caattcggcg tatggatata ttcgcagcag gttgttgtct ggaagatatg ctcctggggt
     4441 gagagtttcg gaattctctg tagcaaagga acttggcata agtcgttcgc cggtgcggga
     4501 ggctatttcg catctcataa gcgaaggctt gctgcagaaa ctgcctggct atggtgcgta
     4561 tgtaaaaatt cctgaccggc gggaaattga agaactgatg gtcgttcgag agcttatgga
     4621 ggagtatgtg gttacgaagg ctgccagctg gtctgaccca aaccagattg aagaattgca
     4681 gaaaatatgt gatgaacttc atggattagc atgccaggcg agacacaacg acatcgaaga
     4741 taaaattatc gacagattaa ttgagcttga ttttgcattt cacaccaggc tgtacgaatt
     4801 agccgacaat cgtcagataa ttcaaatcat gaaaaacgcc agaattctgt cagccatcct
     4861 ttctacctat cagaaggatt ttttcaatct ttcagaaata gctcacatct ggcgggaaca
     4921 tacgcgcatt ttacagtcaa tcagacgccg caatccacag aatgcacgct attggctgaa
     4981 atgccaaatt cggcgaagtc gccaactata taaatcaggc tatgaaaaat tgatggaaaa
     5041 tccgagattc agggaattac gggaattatc gaaagttgct aagagttgat gaattatagt
     5101 ttggcaagtc ttgatttatg ttggggttaa tcttatatat aatgcagacg tgtggttggg
     5161 cggatgaact ataatctttg acttaaaagt ctcacgacta tggttttgtt atattataga
     5221 gtagaacctg tcccaaaaac cttggctgcc tgcaatcggc tcttttgcag cctggcggcg
     5281 tcaga
//
LOCUS       DAONFI010000155         5249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18565_length_5249_cov_3.515017, whole genome shotgun sequence.
ACCESSION   DAONFI010000155 DAONFI010000000
VERSION     DAONFI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18565_length_5249_cov_3.515017"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLK08_06050"
     CDS             complement(<1..673)
                     /locus_tag="PLK08_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53095.1"
                     /translation="MENSDENKTEEIVKPKKRKHKRRWLMRSIHLLVILGLVGFAAFV
                     TIRYWLAPQIAKQMTADFIQTYWVGTVEIVDVDVDSDFDAIVAGSVSLRDNAGREWVR
                     IKHLRLFFRDWPSVHPVLYGIRVESPQVTIFCDNGSINLPVLAKPRETTTTAGSSTHF
                     QIEWTDITDVWLELVQTQVKDAIEPSPLPELRNMTVKGYSSLRGWVNFQFLPDDVVAH
                     AELAGQ"
     gene            complement(716..3124)
                     /locus_tag="PLK08_06055"
     CDS             complement(716..3124)
                     /locus_tag="PLK08_06055"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HPS53096.1"
                     /translation="MQIEFTQLHCTYCLWLLATAAAAGLLWSGILRFKRGKIYSGIAA
                     VTLAVAGFVAWWILCNRLAGTATVAGLLVACVVFFYAERYADVSAKTATVLTLLRLAV
                     LGMLLLILFKPTLVLQPARDALSAANLKILLDSSASMSRVDDGEKTTRYGQSVDVLKN
                     ILPKVQSHAEIEYFTFADFPKQQANVKSVSAVKPAGKITDIVGAVRFSGMADKDLRPA
                     AVLLISDGCHNALSTLAEMGRCRQSVFALGVGRADEKLNRPVGRGAMVENFSVPFVVP
                     LNRETRITATVRMIDMAGRKCTAVLYEDTQPLGQVDITPAGSDVRRNAEFVWRPTGGQ
                     GSGDVRNLRVEVTCQDDADTNDNFCDAAVLIRRPAMKILIVEGTIRPEYKYLRRVLAG
                     DANMQVASFVRMSENRFWAQGSIDGVEQKSLPSDEKDFRQFDVVILGDVAAEFFLPRQ
                     MEGMQNFVSAGGGIIMPGGGSSFSAGGWQDTPLADVLPVQMNAAGQIAKPFDMTLTAT
                     GVNHPIFVGLAKSFNSPAGRVAVPLPPLEGCVRTGTVKAAAELLAVNPAADNATVMAV
                     QNYGKGRAAAFTADTTWRWYLSAQRMGESSPYVKFWLQTVRWLAGSETSKKDGKKIAR
                     VIVRSDRPCVRVGDGEVKFSGVIIDGDAANPLKVQYEIRGEGRYRQPVIFTANRAGEL
                     SASWTPQQPGEYELVRVSPDNASPRQIKIRIEQSDAENTICSRNDAFLSGVAAATDGF
                     YTLSGNEDELAGRLINHLERLRNQAAKSSIKRIDAYNFPLILIMAVGLLTAEWVLRHK
                     KSLR"
     gene            complement(3114..4763)
                     /locus_tag="PLK08_06060"
     CDS             complement(3114..4763)
                     /locus_tag="PLK08_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPS53097.1"
                     /translation="MIFFYLKRYVAFAIIMLTFLCAGCGMPPAVGKPDSYEQDLTPPT
                     GVSPRGAVVFMVDGVNADIFDQMLQKGELPSINKYLVSRGAYVQKALASTPSITLANE
                     TALTTGCLPGNNNIPSVTWFDRYELVWRNYATVDQKDMLDGDHQKPTIFQQFPDDFTV
                     SIFFQSHKGVTKFFENWQSAGPPYFFGMYDVVDRLTLYRTGEMMRMSRKTGKFPQIVY
                     IYLLMPDFMGYHNGIESQDYRYALKHTDYQLGRVLGDMERAGWMDGVYFIFVSDHGLL
                     EVTKHFHVDDFLQNTLKLDVAQKHRREKHPLAERQAYYNQYPCVTYGNGDRFWAVSLR
                     KPAAEAGKFEPWNVRPTEADMRNYPNRANARIDVIARFIAESAVDVVAWADGNGGVNV
                     ATRGGQVTFRQPDGNGAAIEYKVVHGTDPLDWGRYGIKDGQKLLPREWMERTHETSYP
                     GLPADICAYFRCKRCGDIALFAMPGYDFNNVHRAGHGGLRPGEMFVPLIIAGPGIAQQ
                     KIKYARTIDVVPTILELLGKSPMQVDGKALPLKKNKPQNAD"
     gene            complement(4760..>5249)
                     /locus_tag="PLK08_06065"
     CDS             complement(4760..>5249)
                     /locus_tag="PLK08_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53098.1"
                     /translation="PETSEVSTAAIEKAVVIPCGIFPQKGVLETYEGTSAGLDQIANF
                     LASSLALPTVVTQIPFDIPAAIDKLKTNTPGFQLKNVRVSEYAADSYMSGPYSPNFFE
                     TEHGCDFLKEHVEYVTQASIKFQGPHGRVTVTLTPKACFRYSIANEDDKNIVQAALRK
                     LL"
BASE COUNT         1216 a         1478 c         1320 g         1235 t
ORIGIN      
        1 attggccggc aagctcggcg tgtgcgacaa cgtcatcagg cagaaactgg aaattcaccc
       61 agccgcgcag gctggaatat cccttgacgg tcatattcct cagctcaggc agcggcgatg
      121 gttcaatagc gtccttgacc tgggtctgta cgagctccag ccacacatcc gtgatatccg
      181 tccactctat ctgaaaatgg gtactgctgc cggcggtggt ggtggtttca cgcggcttag
      241 ccagtacggg cagattgata ctgccgttgt cgcagaaaat cgtcacctgc ggcgattcga
      301 cgcgtatgcc gtacaatacc ggatgcacgc tcggccagtc gcggaagaac aatcgcaaat
      361 gttttatacg cacccattct cggccggcgt tgtcacgcaa tgaaacactg ccggcaacta
      421 tggcgtcgaa gtcggaatcc acgtcgacat ccacaatttc aacagtgcct acccagtacg
      481 tctggatgaa gtcagccgtc atctgtttgg ctatctgcgg agcgagccaa taacgaatcg
      541 tgacaaacgc ggcaaatccc acgagaccaa gaataacaag aagatggatg gaacgcatca
      601 gccacctgcg tttatgcttc cgctttttcg gcttgacaat ttcttccgtt ttgttttcgt
      661 cactgttttc catggcggat actcatttta tcagaagcta tataaaatta aaaaactatc
      721 gcaaagattt tttatgccgc agtacccatt ccgcagtcag cagcccgacc gccatgataa
      781 gtatcagcgg gaaattatag gcgtctattc gttttatgga gcttttagcc gcctggttgc
      841 gaagccgttc caggtgattt attagacggc cggctaattc gtcttcattg ccgctcaagg
      901 tataaaaccc atccgtagcc gctgcgacgc cagacaggaa tgcgtcattc cggctacata
      961 tggtattttc cgcatcagat tgttcgatgc ggattttaat ctgccgcggc gacgcgttat
     1021 ccggcgatac gcggacaagt tcgtattcgc caggctgttg cggcgtccat gaagcggata
     1081 attcgccggc cctgttcgcc gtaaaaatta caggctgccg atatctgcct tcaccgcgaa
     1141 tttcatattg cacttttagc ggattagccg catcgccgtc gataatgacg ccgctgaatt
     1201 ttacctcgcc gtcgccgacg cgcacgcacg gccggtcgct ccgcacaatt acacgtgcga
     1261 tttttttccc gtcttttttc gacgtctcac tgccggccag ccatcgaacc gtctgcagcc
     1321 agaattttac atacgggctc gactcgccca tcctttgagc cgaaagatac caccgccacg
     1381 tcgtatcagc cgtaaacgca gccgctctgc ccttgccgta attctgcacc gccataaccg
     1441 tagcattatc agcagccgga ttgacggcta acagctcggc agccgccttg accgtgcctg
     1501 tccgaacgca tccttccaac ggcggcaacg gcacagccac ccggccggct ggagagttga
     1561 aacttttcgc cagaccgaca aaaatcggat gattcacgcc agtcgccgtc agcgtcatgt
     1621 caaacggctt ggcaatctgc cctgccgcat tcatctgaac cggcagtacg tcggcaagcg
     1681 gcgtatcctg ccagccgccg gctgaaaagc tgcttccgcc gccgggcatg atgatgccgc
     1741 cgccggctga tacaaaattt tgcatgcctt ccatctgacg cggcaaaaaa aactccgccg
     1801 ccacgtcacc gagtatcact acgtcgaact gccgaaaatc cttttcgtca gacggcagcg
     1861 atttctgttc cacaccgtcg atactgccct gtgcccagaa acgattttcg ctcatcctta
     1921 cgaacgacgc aacctgcatg ttcgcatcgc cggctaacac gcgacgaaga tacttgtatt
     1981 caggccttat cgtgccctcg acaatcagta ttttcatcgc aggtctgcga atgagaaccg
     2041 cagcgtcaca aaagttatca ttcgtatcgg catcgtcctg gcaggtcact tcaacgcgga
     2101 gatttcgcac atcaccgctg ccctgcccgc cggttggccg ccatacaaat tcagcattac
     2161 gccgcacgtc actgccggct ggcgtgatgt cgacctggcc gaggggctgt gtatcttcat
     2221 acagaacagc cgtacatttt cggccagcca tatctatcat gcgaactgtc gcggtaattc
     2281 gcgtctcacg attcagcggc acgacaaatg gtactgaaaa attttcaacc atcgctccac
     2341 ggccgacggg tctgttgagt ttttcgtctg ccctgccgac gcccagcgca aagacgcttt
     2401 gacggcatct tcccatctca gcaagtgtgg acaaggcgtt atggcagccg tcgctaatca
     2461 gaagcactgc agcggggcgt aaatctttat ccgccatgcc cgaaaatcgt accgcgccga
     2521 ctatgtcggt gattttgcca gccggcttga cggcagaaac agatttgaca ttagcctgct
     2581 gctttggaaa atcggcaaaa gtaaaatatt cgatttcggc atgtgactgt actttgggca
     2641 gaatattttt gagtacatcg acagactggc catagcgtgt cgttttttcg ccgtcatcga
     2701 ctctcgacat tgacgcggat gaatcaagca gtattttcaa atttgccgcc gatagtgcat
     2761 cgcgggcagg ctgaagaacc agcgtcggtt tgaacagtat gagcagcagc attccaagca
     2821 ctgccagccg taaaagtgtc agaactgtcg cagttttcgc ggaaacatcc gcatatcgct
     2881 ccgcgtagaa aaacacgaca cacgcaacca gcaggccagc aaccgtagcc gtgccggcca
     2941 gccggttaca taaaatccac catgcgacaa atccggcgac ggcaagagta actgccgcga
     3001 tgccactata aatcttcccg cgtttaaatc gcaaaattcc ggaccacagc agcccggccg
     3061 cagcagccgt cgccagcagc cagagacaat acgtacaatg aagttgagtg aattcaatct
     3121 gcattctgcg gtttgttttt ctttagcggc agtgctttgc cgtcgacctg catgggcgac
     3181 ttgcccaaaa gctccagtat cgtcgggacg acgtctatcg ttcgtgcata tttgattttt
     3241 tgctgcgcaa tgcctgggcc tgcgataatc agcggtacaa acatttcgcc cggccgcaat
     3301 ccgccgtggc cggcacggtg cacattattg aagtcgtaac ctggcattgc gaaaagagcg
     3361 atgtccccgc aacgcttgca gcggaagtac gcgcagatgt ccgccggcaa gcctggatat
     3421 gacgtttcat gggttcgttc catccattcg cgcggcagaa gtttctggcc gtcttttatg
     3481 ccgtatctgc cccagtcaag cgggtcagtc ccgtgcacaa ccttatattc tatcgcagcc
     3541 ccattgccgt caggctggcg aaacgtaacc tgtccgccgc gagttgcgac atttactccg
     3601 ccgttaccgt cggcccacgc cacaacgtca acggcagact cggcgataaa tctggcgatg
     3661 acatctatac gggcgtttgc tcgattggga tagttgcgca tatccgcctc ggtcggccgg
     3721 acattccagg gttcgaattt gccagcctct gccgccggct tacgaagact gacagcccag
     3781 aatcggtcgc cgttgccgta tgtgacgcac ggatactgat tataatatgc ctgccgttcg
     3841 gcgagaggat gtttttcgcg acgatgtttt tgggctacgt ccagcttgag cgtgttttgc
     3901 aaaaaatcgt cgacgtgaaa atgcttcgtc acttcaagaa ggccatggtc gctgacgaat
     3961 atgaaatata cgccgtccat ccagccggct cgctccatgt ctcccaaaac cctgccaagc
     4021 tgataatccg tatgtttcag cgcataacga taatcctgcg attcaatgcc gttatgatag
     4081 cccatgaaat ccggcatcag cagatagatg taaacgattt ggggaaactt gcccgttttg
     4141 cgcgacatgc gcatcatttc acccgtacga tagagcgtca ggcggtcgac gacgtcatac
     4201 atgccgaaaa aatacggcgg gccggctgat tgccagtttt caaaaaattt cgttacgccc
     4261 ttgtgagatt gaaaaaatat cgatacggtg aaatcatcgg gaaattgttg gaaaatcgtc
     4321 ggcttttggt ggtcaccatc gagcatgtct ttctggtcga cggttgcata gtttcgccag
     4381 actagttcgt aacggtcaaa ccacgtgacg gacggaatat tgttattgcc cggcaggcag
     4441 ccggtagtca gcgcagtttc attcgcaagt gttatcgacg gcgtagacgc aagagccttc
     4501 tgcacatacg caccacgcga aacaagatat ttgttaatcg acggcagttc gcctttttgg
     4561 agcatctggt caaaaatgtc ggcatttacg ccgtccacca tgaatacgac tgcgcctcgc
     4621 ggcgatacgc cagtcggcgg agtcaaatcc tgttcataag agtccggttt gccgacagcc
     4681 ggcggcatgc cgcagccggc gcacaaaaac gtcagcatta ttatggcgaa ggcaacatac
     4741 cttttcagat aaaaaaaaat cataacagtt tccgcagggc agcctgtact atgtttttat
     4801 cgtcttcgtt tgcgatgctg tagcgaaagc aggctttggg cgtcagcgtc acggtcacac
     4861 ggccgtgcgg cccctgaaat ttgatgctgg cttgcgtgac gtattccacg tgttccttca
     4921 gaaaatcaca gccgtgttcg gtttcaaaaa aattgggcga atacggcccg gacatatagg
     4981 aatcggcggc gtattcgctc acccgcacgt ttttgagctg gaagccgggc gtatttgttt
     5041 tgagcttgtc tatcgcggct ggaatatcga atggtatttg cgtgacgacc gtcggcagcg
     5101 caagcgaact agccaggaaa ttcgcaatct ggtccaaacc ggctgacgta ccctcgtaag
     5161 tctccaaaac gcccttttgc ggaaaaatac cgcacgggat gacgacggct ttttcgatgg
     5221 cggcggtcga aacttccgac gtttcggga
//
LOCUS       DAONFI010000156         5229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18656_length_5229_cov_4.065713, whole genome shotgun sequence.
ACCESSION   DAONFI010000156 DAONFI010000000
VERSION     DAONFI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18656_length_5229_cov_4.065713"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLK08_06070"
     CDS             <1..1223
                     /locus_tag="PLK08_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53099.1"
                     /translation="KRRNKGMILVISLWAVAIMAVIAIAVCGTVYMSQGQLRAVRTVN
                     CNELATQSAISAVCTQLLADTSGATTASNFPLTINDVEVTLRDSTIQDESARLNANTV
                     TVEMFAKLNGMNESAAKKFVAAREKLAELRSDSKDEKKANWPIADLPELAKILTDATS
                     LKYAANSYTTADDADWYNAVGVCSEVAAIMKNLTVYSRQKNTDSSGQARVNINLAAAD
                     TLTQRLGDVLTSEQITAMVQSRAQNPFTNIVELLTRQYEPVIDEKTVTVRIDEDAFRK
                     IADRVTVTDKTVLLGLVDVSSASQDVLRTLPGLSENDVTAITSWQQFKASPTDLAQNG
                     IASLLDVLSADTLAAIAPYITTRNHQYRCVFIKNYAGNLEQYTACVIEKSDRNFGTIF
                     RYDWSVFASVGDNR"
     gene            1261..2736
                     /locus_tag="PLK08_06075"
     CDS             1261..2736
                     /locus_tag="PLK08_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53100.1"
                     /translation="MLGIDISAGSLRVVELELDGGRFVLRRCGQAAVKEHAEPQDVAA
                     VLEGLLNQNKIDERQGCFSLPPSAGFVRYTSAGENSPQFGLDHVTDSWTCISGEQVAG
                     VAARREVEKISVIAERAGVKLSGITLRTMACMKALGLWDEEYPQPMLGIVVDDSTVTM
                     AFRDGQAIKDIQSRPLANCEKNEVLQAIGQLFRLMKMARADFHPSKILLLWDACTEED
                     RLSLAGAMEAEVFLRRNNWRPMDGQADLFDLGGRYIAAVGLAMQAIEHAGQHGKADAM
                     PPRNFNFIIRPQKNRKQLKLTWKQWGKLTAVFIAVVAGVLLTEVFVKKISLWNLQQRY
                     SRLEPQINQRQEIRKLITQARPWMNPSLGGSRPAYRNVYDTINNLLPNTDQAYVTWLE
                     IRMDNDLGKQVVRIDGRSTGTDSLYEFVSRLNNSLLFEKAELGTVTDIPDANNPFGKQ
                     WTVTFEVANPLLAPTSAPTSAPSVVPATRQARNTIQPSGMK"
     gene            2740..3837
                     /locus_tag="PLK08_06080"
     CDS             2740..3837
                     /locus_tag="PLK08_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53101.1"
                     /translation="MFETRREKLLLVLAMILLILLVADSLVFTPLGKWLDNIDSEAQR
                     LRTQISQVVSPKADDHKIQVDRNNIRLSSEQGQNLFRQYLESQAGMNVIKSSTKKSKT
                     DLAGQNGLSEITYELKLEGSAEALRSFLDNLDSSDEFLRILSLQIATVSQEDQTINMK
                     LVVSTLSRESQGLQDDLAETQRQTRFPLHQPLLPLLPLRKNIFFPGGGKVGRASMPNE
                     FILIGTTTQAKEPSACIEFVGNKESVWVKAGDSVGYATIKQITSSGMEMELPGGEIKH
                     LPISKSSATLWQDSTAYFDNFELVGISESDGRLFALVRPSGEERVFRLGVYDRLGQGR
                     IVKIEKDSITVRTGDNRQTITIGGWYISDLQ"
     gene            3866..4534
                     /locus_tag="PLK08_06085"
     CDS             3866..4534
                     /locus_tag="PLK08_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53102.1"
                     /translation="MRNIFIIICLWFCMIAGCSASLPQTSSQDNLIAPILPAGEKDSD
                     KNKIATKWYIDWADEGDISDKDIKDFLFIASPKGRDWRAPAGPDGYLLRVILLDRDEK
                     PCRKDGRIQAFLIRNHGSRDAEPICAWDISPTMLSDYYDESAWPGYVLPLDWGKGPKK
                     TDGCYLLVIRWISPDGKARLTRNTIFEEAVAVETNTYRRSVNAGGSSSNPVGGGKSAG
                     SGAK"
     gene            4470..>5229
                     /locus_tag="PLK08_06090"
     CDS             4470..>5229
                     /locus_tag="PLK08_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPS53103.1"
                     /translation="MLAAVVVILSAAGNLPAQEQSKPETVASAAAAPVPTPAPAPIKA
                     NFNFKNAQPSSILEGLEQLYGVPFVPEVQLTERITISSGQPITLAEAIQLLDSTLRRQ
                     GATVRMEGGAVRIMPLSLATTRVEMITLKYADPANVATIIEKLFQSRDMLSESDRKSM
                     NTLSQMLSGADKERLATLSSKIQITAVPYPRLKAVVLRAPDAIIGTIREFIINELDKP
                     EQTAAATEQATPPAPPQPQKTRIYKLNYVPASHVV"
BASE COUNT         1396 a         1405 c         1377 g         1051 t
ORIGIN      
        1 tgaaacgcag gaataagggc atgatactgg tcatttcgct gtgggcggtg gcgattatgg
       61 cagtaatcgc gatagccgtc tgcggcacgg tctacatgtc gcagggacaa ctgcgagccg
      121 tccgcaccgt gaattgcaac gaacttgcaa cgcagtcagc catatccgcc gtctgcacgc
      181 aactgctggc tgatacgtcc ggcgccacaa ctgcgtcaaa ttttccgctg actattaacg
      241 acgtcgaagt aactctgcgc gacagtacca tccaggatga atccgcccgc cttaacgcca
      301 acactgttac cgtggaaatg ttcgcaaaac tcaacggcat gaatgaatcg gcagccaaaa
      361 aattcgtagc cgcgcgtgag aaattggcgg aattgcggag cgattcaaag gacgaaaaga
      421 aggctaactg gcccatagcc gacctgcctg aactggcgaa aatattgacc gacgcaactt
      481 cactcaagta cgccgccaac agttacacca cggcagatga cgctgattgg tacaacgcag
      541 tgggcgtgtg ttctgaagtg gcggcgataa tgaaaaatct gaccgtttat tcccgccaga
      601 agaatacaga ctcatccggc caggctcgcg tcaatatcaa cctggcggct gccgatacgc
      661 tgacgcaacg actcggcgac gtacttacct cggaacagat aaccgctatg gtgcagagcc
      721 gtgctcaaaa tcccttcaca aacatcgtgg aactgcttac ccgtcaatat gaaccggtta
      781 tcgatgaaaa aaccgtcact gtcagaattg acgaagatgc attccggaaa atcgccgaca
      841 gggtaacagt cacggataaa accgtgctgc tgggccttgt tgatgtaagt tcggcatcgc
      901 aggacgtatt gcgaaccctg cctggccttt ctgaaaacga tgtaactgcg ataacttcat
      961 ggcagcagtt caaagcatcc ccaaccgacc tggcccaaaa cggcatcgcc agccttctgg
     1021 acgtactttc ggcagatacg ctggcagcta tagcccccta tataactacg cgaaatcatc
     1081 agtatcgctg cgttttcata aaaaattacg ccggcaacct ggaacagtac accgcctgtg
     1141 taattgagaa gtcggacaga aatttcggca cgatattccg atacgactgg agcgtgtttg
     1201 catccgttgg cgataatcgc taaaataatg ccgccgcggc taaattgtgg ttttaaaaat
     1261 atgctaggta tagacatcag cgccggaagt ttgcgagtcg tcgagcttga gcttgacgga
     1321 ggcagattcg tattgcgccg atgcggacag gcggcggtaa aggaacacgc cgagcctcag
     1381 gacgtcgcag ccgtcctgga aggcctgctg aatcagaata aaattgacga acgacaaggc
     1441 tgtttttcac tgccgccgtc tgcaggcttc gtacgataca cgtcagccgg cgaaaattcg
     1501 ccgcaattcg gactggacca cgtgaccgac tcgtggacgt gcatttccgg cgaacaggtc
     1561 gccggcgtcg cagccaggcg tgaagtggaa aaaatttccg taatagcaga gagggccggc
     1621 gtaaaactct ccggcataac gctgcgaaca atggcttgca tgaaggcgct gggcttatgg
     1681 gacgaagagt atcctcagcc gatgctcggc atagtcgtgg acgactcgac ggtaaccatg
     1741 gccttcaggg acggccaggc tatcaaggac atccagtcac gcccgctcgc aaattgcgaa
     1801 aaaaacgaag tgcttcaggc cataggccag ctattcaggc tgatgaaaat ggctcgcgcc
     1861 gattttcatc cgtcgaaaat tctgctgctg tgggatgctt gcactgaaga agaccgcctt
     1921 tcgctggcag gtgcgatgga ggctgaagtt tttttacgtc gcaataactg gcggccaatg
     1981 gacggccagg ctgatttgtt tgacctgggc ggccgataca tagcagccgt cgggctggcc
     2041 atgcaggcta tcgagcatgc cggccagcat ggcaaagccg acgcaatgcc gccgcggaat
     2101 ttcaatttca tcatacggcc gcaaaaaaac aggaaacagt tgaaactcac atggaaacag
     2161 tggggcaaac tcacggcggt tttcatagcc gtagtcgccg gcgtactgct gacggaagtt
     2221 tttgtaaaaa aaatatcgct gtggaatctt cagcagcgat acagtcgcct cgaaccgcag
     2281 ataaaccagc ggcaggaaat acggaagctg ataacgcagg cccggccgtg gatgaatccg
     2341 tctctaggcg gcagcaggcc ggcgtatcga aacgtctatg acacgattaa caatcttctg
     2401 ccgaatacgg atcaggcgta tgtaacatgg ctggaaatcc gcatggataa cgacctgggc
     2461 aaacaggtgg tcagaataga cggcagaagc acaggcacgg attcgcttta cgagttcgtt
     2521 tcgcggctga acaattcgct actgttcgag aaggctgaac tgggtacggt caccgatatt
     2581 ccggacgcaa acaacccgtt cgggaaacag tggaccgtca cgttcgaagt tgccaacccg
     2641 ctgcttgcgc cgacttctgc tccgacgtct gctccgtccg tcgtaccagc gacccggcag
     2701 gcacgaaata cgatacaacc atccggaatg aaatagacaa tgtttgaaac gagacgtgaa
     2761 aaattgctgc ttgttctggc gatgatactg ctcatcctgc tggtggcgga ctcgcttgtg
     2821 ttcacgccgt tgggtaaatg gctggacaac atcgattcag aggcccagcg cctgcgcacg
     2881 cagatatcgc aggtcgtttc gcctaaagcc gacgatcaca aaatacaggt cgaccgcaac
     2941 aacatacgtc tttcaagcga gcagggccag aatcttttcc ggcagtacct ggagtctcaa
     3001 gccggcatga atgtgataaa aagttcgaca aaaaaatcca aaaccgacct ggccggccag
     3061 aatggccttt cggaaattac atatgaactg aaactggaag gctcagccga agcactacgc
     3121 tcatttctgg acaacctcga ctcaagcgat gaatttctgc ggattctttc tttgcagata
     3181 gccaccgtct cccaggaaga ccagacaatc aatatgaaac tggtcgtttc cacgttatcc
     3241 cgcgaatcgc aaggccttca ggacgacctg gcggaaaccc agcgtcaaac ccgctttccg
     3301 ctgcatcagc cgctattgcc gctactgccg ttgcggaaaa atattttctt tccaggcggc
     3361 ggcaaagtcg gccgggcatc catgcccaat gaattcatac ttatcggcac aaccacgcaa
     3421 gccaaagaac cgtccgcctg cattgagttc gtcggcaaca aggaatccgt atgggtaaaa
     3481 gccggcgaca gcgtgggtta cgccacgata aaacagatta catccagcgg catggaaatg
     3541 gaactgcccg gcggagaaat caagcatctg cccataagca aaagcagtgc aacgctctgg
     3601 caggacagca cagcctattt tgataatttc gagcttgtgg gcataagcga atcggacggc
     3661 agactttttg cattggtacg gcccagcggc gaagaaaggg ttttccggct gggcgtttat
     3721 gaccggctgg gccagggcag aatcgtaaaa atagaaaaag acagcataac agtcagaacc
     3781 ggcgataacc ggcagacaat cactatcggc ggatggtata tttccgacct gcaatagcag
     3841 acagtggcta aaaacgagat atcctatgcg taacatcttt ataataatat gtttatggtt
     3901 ctgcatgatt gccggctgct ccgccagcct gccgcaaacc tccagccagg ataatcttat
     3961 agcacccatc ctgccagccg gcgaaaaaga ttccgataaa aataaaattg caactaaatg
     4021 gtatattgac tgggctgatg aaggggatat ttccgataaa gatataaagg attttctgtt
     4081 tatagccagt ccgaaaggcc gtgactggcg tgcgccggct ggccctgacg gatatttgct
     4141 gcgagtcata cttcttgacc gcgacgaaaa gccgtgccgg aaagacggca gaatccaggc
     4201 gtttctgata cgcaatcacg gcagcagaga tgccgagccg atatgtgcat gggacatatc
     4261 gccgacaatg ctgtctgatt attatgacga aagtgcctgg ccgggttacg tcctgccgct
     4321 ggattggggc aaaggcccca aaaaaaccga cggatgttat ctgctggtca tcagatggat
     4381 aagtccggac ggaaaagcac gattgacgcg taatacgatt ttcgaggaag ctgttgcagt
     4441 cgaaaccaat acatatcgca ggtctgtcaa tgctggcggc agtagtagta atcctgtcgg
     4501 cggcgggaaa tctgccggct caggagcaaa gtaagcctga aactgtcgcc tctgccgcag
     4561 ctgcgcctgt tccgacgcct gcacctgcac ccatcaaggc gaatttcaat ttcaaaaacg
     4621 cccagccgag ttccatcctc gaaggcctcg aacagctcta cggcgtgccg tttgtgccgg
     4681 aagtacagct gaccgaacga atcaccatct ccagcggcca gcccataacg ctagccgagg
     4741 caatccagtt gctcgattcc acgcttcgcc gccagggtgc gaccgtccgc atggaaggcg
     4801 gcgccgtgcg aatcatgccg cttagcctgg ctaccacgcg tgtggagatg ataaccctca
     4861 aatacgcaga cccggccaac gtagccacaa tcatagaaaa acttttccag tcacgcgaca
     4921 tgctcagcga gtccgacagg aaaagcatga ataccctctc gcagatgctt tccggcgccg
     4981 acaaggaaag actcgcaacc ctcagcagca agatacagat aacagccgtg ccgtatccgc
     5041 ggctgaaagc agtcgtccta cgtgcgccgg acgcgattat cggcacaatt cgcgaattca
     5101 taatcaacga gctcgataag ccggaacaga ccgccgccgc gacggaacag gctacgccgc
     5161 ctgcgccgcc ccagccgcag aaaactagaa tatataaact gaattacgtc cctgccagcc
     5221 acgtcgtag
//
LOCUS       DAONFI010000157         5198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18806_length_5198_cov_3.776201, whole genome shotgun sequence.
ACCESSION   DAONFI010000157 DAONFI010000000
VERSION     DAONFI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18806_length_5198_cov_3.776201"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1038)
                     /gene="larE"
                     /locus_tag="PLK08_06095"
     CDS             complement(208..1038)
                     /gene="larE"
                     /locus_tag="PLK08_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPS53104.1"
                     /translation="MDNQLEHTGSLDDKLAACKDMICSKQRVVVGFSGGVDSSLLLYL
                     AKTSLGEANVLAVTAAGYFHHPSETALAGKLASQLAVPWQTINMDSLQDEKIISNPPD
                     RCYWCKKLIFSMMQDIAQKENYSAVLSGANADDASDFRPGLRAEDELGILRPLLAAGI
                     TKSEIRRLARKFDLPNFDKPAQPCLASRIPYNTRLTKKAFEQIKAGEDILKQHFGFDE
                     CRLRHFSHSASIELPLDRLPLPFEIQMQIITEIRLLGFDEVSIDPHGFRSGSLNEALL
                     "
     gene            1119..2254
                     /locus_tag="PLK08_06100"
                     /pseudo
     CDS             1119..2254
                     /locus_tag="PLK08_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
     gene            2278..2877
                     /gene="accB"
                     /locus_tag="PLK08_06105"
     CDS             2278..2877
                     /gene="accB"
                     /locus_tag="PLK08_06105"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPS53105.1"
                     /translation="MSKKAVKKTASKTAAGKAAKTAVTAACDKEKVRKNVVSGKPASP
                     LLCDVEHLMHIMAANDVTEIDIVDGSRKISLKRGTCAAAQAVAAPAATIAAAAAAPAI
                     VPAAGKAATSAAGTDEDENLQKITSPMVGTFYSSPSPDSESFAKVGDAVNDDTVVCII
                     EAMKVMNEIKAEIKGTIVEVCVKNAQPVEFGQVLFKVKP"
     gene            2907..4259
                     /gene="accC"
                     /locus_tag="PLK08_06110"
     CDS             2907..4259
                     /gene="accC"
                     /locus_tag="PLK08_06110"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPS53106.1"
                     /translation="MISRVLIANRGEIALRIIRACKELGLETVAVFSEADREASYLHL
                     ADDRICIGPAPSSESYLNIPRIIAAAEIADVDAIHPGYGFLAENAHFADVCRDCKIKF
                     IGPTVENMMLLGDKIAAKKLAIKAQVPTTPASEGAIETLDEALDCARHIGYPVALKAA
                     AGGGGRGIRFAHNEATLRNIFPQAQTEAKIAFNDGRVFMEKCIENFRHIEVQILADEH
                     GNVVHCWERECSAQRRNQKVIEQTPAPNLPQKVREDICEASARLAREAGYQNAGTVEY
                     MLDQNNNFYFLEVNTRIQVEHCITEMVTGLDLVKWQLRIAMGEKLDIKQKDIRQSGTA
                     IECRINAEDPARNFMPSPGVVKAFHAPGGLGVRWDSHVYQGYTIPPTYDSMVGKLIVH
                     RPSRSEAIAVAKRALDEFVISPTKTTIPLCRAILEHEKFVKGQWDTKMIEREMLPPRE
                     "
     gene            4483..>5198
                     /locus_tag="PLK08_06115"
     CDS             4483..>5198
                     /locus_tag="PLK08_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53107.1"
                     /translation="MKFLVELTADVVEASRVPQGTKVLSYGPNSTGGTIIRPGGRGCY
                     PAFWVRDYAMSLETGMITLEEQEHALRLTAATQQEGDWLTISGSCVPHGSIVDHVSLE
                     GKAIFFPGTLDDYENQGGIWGKRPSLDDHFYFIHMAWYLAVAAKKPEILSLEVGGRTI
                     LERLALAFAVPPSDSDTCLVRCDENTRGVSFGFVDAIMHTGKLLFCSLLKYRAAEEMA
                     ELFKLAGDDTNAARYVSVAA"
BASE COUNT         1254 a         1263 c         1448 g         1233 t
ORIGIN      
        1 gcccgcaaaa ttgctcgatt tcggcgacgc ccatgttttt gggataggtt ctatataatt
       61 ccacaggtct tgatacgtaa agattccagc atgaacgtac atgccgctac acgacagcat
      121 atcaaaacct gtggggtttt cccaaaaatc ttttaattgc cgatggaaaa atatatgaca
      181 ccgtacagtc gggttatcgg ctgattttta gagcagggct tcgttcaacg agccggagcg
      241 gaagccgtgc gggtcgattg aaacctcgtc gaatcccagt aatcgtattt ccgtaatgat
      301 ttgcatttga atttcaaacg gcagcggcag acgatcgaga ggcagttcta ttgatgcgct
      361 atgtgaaaaa tgtcttagcc tgcattcatc gaagccgaaa tgctgtttta gaatgtcctc
      421 gccggctttt atctgttcga acgccttttt tgtaagccgc gtgttgtaag gtatgcgact
      481 tgcgaggcag ggctgagccg gtttgtcgaa attcggcagg tcgaatttac gggctagccg
      541 tcggatttcg cttttggtta ttccagccgc caggagaggc cgcagtatgc cgagttcatc
      601 ctcagcccgc aagcccggcc gaaagtcgct ggcgtcatcg gcattagccc cgctgaggac
      661 ggcactgtag ttttcctttt gtgctatgtc ctgcatcatg gagaagatga gttttttgca
      721 ccagtagcat ctgtccggcg gatttgagat tattttttcg tcctggaggc tgtccatgtt
      781 tatggtttgc cacggcactg cgagttggct ggccaatttg ccggctagtg cggtttcgga
      841 agggtgatga aaataaccgg ccgctgttac cgccagcacg ttagcctcgc ctaaagacgt
      901 tttggccagg tacagcaaca gcgacgaatc cactccgcct gaaaaaccga caacaacacg
      961 ctgtttactg caaatcatat ccttacaggc tgccagtttg tcgtcaaggc tgccggtatg
     1021 ttccagctga ttgtccatgc cgacattata gctgttttgc agctgatttt cagggtattt
     1081 tctggcaaaa gcagggtaaa aatgtataat atcctgccat gaaagacaat accgacaggc
     1141 aggatgctat atgccggata ttcaggacaa gacgcaggaa ggcgttaaaa ctggcaggat
     1201 gccaagcctc ctgcaacacg attctgattt ccaatccgtt cgacgttgaa tatctgaccg
     1261 gctttaatgg cggcgacagt tatcttctta tcggccagga tgttgtaacg cttgtgaccg
     1321 acagccgcta tactgagcag gctggcagcc agtgtgggcc ggacgttcag attttcaccc
     1381 gtaaaagtcc tatgagcaaa gcggttgcct tggcattgaa aggcagcggt acaagacggc
     1441 tgggcataca gtctttgcat gcgacggttg ctttggccga gtctcttcag gaattgttgg
     1501 ggaaaaaggc tgtaattccg ctaaaaccgt ttatttacca gttgagaatg ataaaagatg
     1561 acgccgagct ggtacttatg cgaaaggctt acaggatagc tgtggagtca ttctgcgaac
     1621 tcgtcggcag gggggcgtcg tattggctgg gccgtacgga attgcagatt gccgccgaac
     1681 tggactacct tatggtctct gccggcgcca cgaagccggc gtttgacacc atagtggctg
     1741 ccggcgaagc ggcgtcaatg ccgcatcatg ccacgtcagc cagaaaaata cgacccggcc
     1801 agggaatatt gctggatttc ggcgccagat atcgtggata ttgcagcgac ttgacgcggg
     1861 tggtgtttgt gggtaaaatt cctccccgta tatcaaggtt ttatgaaacc gtatatgctg
     1921 cgcagcaggc tgcgatagac gcaattcggc cgggcgtaaa ggcttgtcgg gttgacgccg
     1981 ttgcccgcgg cattatcgcg gatgcgggtt acggcgaaaa tttcctgcat agcctggcca
     2041 cggacttggg ctggaagttc atgaaatgcc gactctttcc gcactgagtt cagccgtgct
     2101 aagaaaaggc atggtagtta cggtagaacc ggcgatttac cttccccgcg tcggcggggt
     2161 gcgtatagag gatgacgtgg aaatcacggc gtccggtgcc aggctgcttg gtgcggctgg
     2221 ccgaaaaaat atggatgata tgattttgca gtgagattaa cagggaagga cagaaatatg
     2281 tcaaaaaagg cagtcaaaaa gacggcgtcg aaaaccgccg ccggcaaagc tgcgaaaacc
     2341 gccgtaacgg cggcatgcga taaagaaaag gtaagaaaga acgttgtttc aggcaaaccc
     2401 gccagcccgc ttttgtgcga cgtggaacac ctgatgcata taatggctgc caacgatgtt
     2461 acggaaatag acatcgtgga cggctcgcgg aagatatcgc tcaaacgcgg tacttgtgcg
     2521 gcggctcagg ctgtggcagc tcccgctgca acgattgccg cggcagccgc ggctccggcg
     2581 attgtgcccg cggccggcaa ggctgcgaca tctgctgccg gtacagatga agatgaaaac
     2641 ctgcagaaaa tcaccagccc gatggtcggc acattctatt cctcgccgag tccggactcc
     2701 gagtcgttcg ccaaagtcgg cgacgcggta aatgatgata ccgtcgtctg cataatcgaa
     2761 gccatgaaag tcatgaatga aatcaaggct gagattaagg gcactatcgt ggaagtgtgt
     2821 gtcaagaacg cccagccggt ggaattcggc caggtgctct tcaaggtcaa accctgagcg
     2881 gcctgccgca tgcggagaat taccggatga tttcacgcgt acttatagca aatcgcggcg
     2941 agattgcgtt gcgcatcatt cgcgcctgca aggaactggg acttgaaacg gtggcggtct
     3001 tcagcgaagc cgaccgcgag gcaagttacc tgcatctcgc tgacgaccgc atatgcatag
     3061 gccctgcacc gtcttctgaa agctacctga atattcctcg cataatcgcg gcggcagaaa
     3121 ttgccgacgt cgatgcgatt caccccggct acggtttttt ggctgaaaat gcccattttg
     3181 cggatgtatg ccgcgattgc aagataaaat tcataggccc taccgtcgag aacatgatgc
     3241 tcctcggcga caagatagcc gccaagaagt tagccatcaa agcccaggtg cccaccacgc
     3301 ctgccagcga aggcgccatc gaaacgctcg atgaagccct cgattgtgca cgtcatatcg
     3361 gctatccggt tgccctgaaa gccgctgccg gcggcggcgg acgcggcata cgctttgcgc
     3421 ataatgaagc aactttgcga aacatttttc cgcaggctca gaccgaagcg aagatagcct
     3481 tcaacgacgg ccgagttttt atggaaaagt gcatcgagaa tttccgtcat atcgaagttc
     3541 agattctcgc cgacgaacac ggcaatgtcg ttcactgctg ggagcgtgaa tgttccgccc
     3601 agcgtcgtaa tcagaaggtc atagagcaga cgccggctcc taatctgccg cagaaagttc
     3661 gcgaggatat ttgtgaggca tcagcccggc tcgcccgcga agccggctat cagaacgccg
     3721 gcacggtcga atatatgctc gaccagaaca ataatttcta ctttctcgaa gtcaataccc
     3781 gtatccaggt tgaacactgc ataacagaga tggtgaccgg acttgacctg gtcaaatggc
     3841 agttgcgcat tgcaatgggc gaaaaactcg atataaagca gaaggacatc cgccagagcg
     3901 ggacagccat cgaatgtcgc ataaatgccg aagatcctgc acgaaatttc atgccgtcgc
     3961 ccggcgtcgt caaagcgttt catgcccctg gcggcctcgg cgtgcggtgg gattcgcatg
     4021 tctatcaggg ttacacgatt ccgccgacgt acgacagcat ggtcggcaag ctcatagtgc
     4081 atcggccgag ccgcagtgaa gccatcgccg ttgcaaaacg cgcactggac gaattcgtta
     4141 tatcgccgac gaaaacgacc ataccgctct gccgcgctat acttgagcac gaaaaattcg
     4201 tcaaaggcca gtgggatacc aagatgattg aacgcgaaat gcttccgccg cgcgaatgag
     4261 tatgtaaaaa tcttttggaa tatacgttgt tcaaaggttt ttgtagccca tccccaaaat
     4321 attggcgtta ctgaaaatca agcaattttg cgggctggca aagaagcaaa gcggcgtcat
     4381 atttggtgat atctgccgtt ttttctgatg ctggtctaaa ataataagag aaagactctt
     4441 tttatggact ttaattcgtc tgcttgcata agtggcagag atatgaagtt tctcgtggaa
     4501 cttacggctg atgtcgttga ggcgtctcgc gtgccgcagg gaacgaaagt tttgagttat
     4561 gggcctaatt ctacgggggg aacgataatt cggcctggcg ggaggggatg ttacccggca
     4621 ttctgggtgc gcgattacgc gatgtcgctt gaaacaggca tgattacgtt ggaagagcag
     4681 gagcatgcgt tgcgattgac ggcggctacg cagcaggaag gtgattggct gaccatatcg
     4741 ggcagttgcg ttcctcacgg ttcgatagta gatcatgtca gcctcgaagg aaaggcaata
     4801 tttttccctg gcacgctgga tgactatgaa aatcagggtg gtatctgggg taaacggccg
     4861 tctcttgacg accattttta tttcattcat atggcatggt atcttgctgt tgccgcaaaa
     4921 aagcctgaaa ttttgtcgct tgaggttggc ggcagaacga ttctcgaacg gttggcattg
     4981 gcgttcgcag ttcctccgtc tgattccgat acctgtctag tgcgttgtga cgagaatact
     5041 cgcggcgtat cgtttgggtt tgtcgatgcg ataatgcata ccggcaagct gcttttctgt
     5101 tctctattga aatatcgtgc agccgaagag atggcggaac ttttcaaact tgccggagat
     5161 gatacgaatg ctgccaggta tgtatccgtc gccgctag
//
LOCUS       DAONFI010000158         5160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_18997_length_5160_cov_4.163957, whole genome shotgun sequence.
ACCESSION   DAONFI010000158 DAONFI010000000
VERSION     DAONFI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18997_length_5160_cov_4.163957"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..657)
                     /locus_tag="PLK08_06120"
     CDS             complement(43..657)
                     /locus_tag="PLK08_06120"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPS53108.1"
                     /translation="MSDCILKKSVRDFVEATAAKQPTPGGGSVAGVVGALGTALGEMA
                     LNFTAGKKKFAEHEEYYVVLAERLKKSRDMFMTLVADDMAAFGMYCDASAMPESREKQ
                     QASELALAAAIDVPREMTKLCLAVLYDLKSLADKCNPWLISDLVAGGILAAATAGLCD
                     LNVRINARNLENPQRDEILNASAADRRRAAAIAAEIEEMAGKLL"
     gene            complement(707..3436)
                     /locus_tag="PLK08_06125"
     CDS             complement(707..3436)
                     /locus_tag="PLK08_06125"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPS53109.1"
                     /translation="MKFATILQTAAVVTFLVATLMAGCDDGQPSQIAAAKPADVAASQ
                     PQVAPKVVAEQYLVTSPDIQAVRLSNGMYVIAKAVRKAPVVCVCACVKAGGIYEGKYL
                     GCGLSHLLEHLCAENHDGAQGASADKPKLSDAIGGQDNAYTAKDHTCYYISASSDRMS
                     QCIDYIAAQVADANITRKDFEREHGVVQRELEMGLDDPDRVLFYTHEADFYGRHPAAV
                     PTIGYLAPLAAVSYEDVLAYHARMYVPQNMVFVVAGDIDPAKAVADVIKKFGDFQAGR
                     QPAPVLPPVEPLTSVRRFIRPNEQFQEVAEIIGFQTVPITDRDLYALDVLSYILSNGK
                     ASRLVEHVQFRQKFVTSVETWSDTPAWGGGEFAVAFRCTPGMEDKAEKAIIDQLRRIS
                     KTKVSAEELRRAKMQKISDYVNAQQDVFSIASSLGCDMLLTGDMNFSKNYTDRIQKVT
                     AAQVRQVARKYFNFNRMVVTRIVPRKVFNAAKADAAAGGSSAAEIVTLPNGLRVVLCE
                     SHDVPLVAMAFACRGGILNETAKTDGLGLMMSVLSTRGAGDMTGSEIAAFFNSAGGTI
                     SAIAGDNSYLYSAEVMSDKFGKALDIFADVVLRPTFPQKELDSVKPLLIADIRRSDEN
                     WVQLLMKKHRGDFYAPATPWSLPNSGYEEVIKSATRKMLEDYHKKYILAGESVLAIYG
                     DFDAKNAAAKVRKLFGDMPAGSTSLPAVPVKREISASDETHVYSVPIQQAGIIVAANG
                     PTVKNVEDRLPLMLLDTIISGYKYPCGWLHDQLRGRQLVYVIHATQKNGFLPGAFLAY
                     AGTQPEKAQQVIDIIKANFQKAADYTPTRQELDLAANKIITSELLENQSLGSLATGAA
                     MNVLYGLGVDWPKTLEKKIRSITPQQVRDTAQKYLAGGYVVTVITPKPAAVKK"
     gene            complement(3436..3891)
                     /locus_tag="PLK08_06130"
     CDS             complement(3436..3891)
                     /locus_tag="PLK08_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS53110.1"
                     /translation="MTQTTPASRILVVDDNEQNRELIIAYLDDLEGCTVDTAADGIEA
                     LDKTFGENQPDIILLDVMMPRMSGFEVCRKLKTNPQTRDIPIIMITALNELGDIERAV
                     ENGTDDFITKPVNRLELVTRIKSLLRVRHLKNELDRTLAHFSNLDEKVN"
     gene            4103..5098
                     /locus_tag="PLK08_06135"
     CDS             4103..5098
                     /locus_tag="PLK08_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPS53111.1"
                     /translation="MEYRNLGKTEIRVTPIIYGAWAIGGWMWGPQDESAAVDAIRTSL
                     DAGINAIDTAAVYGFGRSERLVAKAVKGRRDKTVIMTKYGLRWDAGNVGTLRFNTTDL
                     QGHPLKVYRYAGAKSVVEECERSLKNLDTDYIDVYQIHWPDDTTPLEETFGAIEKLMK
                     QGKIRAAGVSNYTTELLADANSVIQLASCQPPYSMVLRNAEKDIIPWCEENQVGVVVY
                     SPLQRGLLTGKITPDYQFAPDDHRRGNVFFTPHNIASVNAFLAKIKPIADAHNATLAQ
                     LVINWTVHRNGITAALVGARNAKQAQENAAALNFTLTSQETQTINNLLDELKIEK"
BASE COUNT         1181 a         1424 c         1297 g         1258 t
ORIGIN      
        1 tcggcgacgc ccatgttttg gggataggtt ctaatagacg cgttaaagca gtttgccggc
       61 catttcttct atttcagcgg ctatcgccgc cgcccgccgc ctgtcagccg ccgacgcatt
      121 taaaatttcg tcacgttgcg gattttccag attgcgtgca tttattcgta cattcaggtc
      181 acataaccca gccgtagcgg cagccagaat cccgcctgcc acgaggtcgc ttatcagcca
      241 cggattacat ttatccgcca gtgatttcag gtcgtacaat acggccaggc ataatttggt
      301 catttcgcgc ggcacatcaa tggcggctgc aagcgccagc tcggaagcct gttgtttttc
      361 gcggctttcc ggcattgcgc tggcgtcgca gtacatgccg aaggcagcca tatcgtcagc
      421 caccaacgtc atgaacatat cgcgcgattt tttcagccgc tcagccaaca cgacataata
      481 ttcctcatgc tcggcgaatt tctttttgcc agccgtaaag ttcaatgcca tttcacccag
      541 cgccgtcccc agcgcgccta caacgcctgc gacgcttccg ccgccaggag tcggctgttt
      601 agctgccgtc gcctcaacaa aatcccttac cgattttttt aaaatgcaat ctgacatcca
      661 gcgttccatc cttcagaatt atcccttacc atatttgccg ttcgactcat ttttttactg
      721 ccgccggttt tggcgtaatt acagtaacaa cgtatccgcc ggcaagatat ttttgagccg
      781 tatcgcgtac ctgctgcggc gttatgctgc ggattttttt ctccagcgtt ttgggccaat
      841 ccacacccag tccgtacagg acgttcatcg cggcgccggt agccagcgag cctaacgact
      901 gattttccag cagttccgag gtgattattt tattggctgc caggtcgagt tcctgacgcg
      961 taggcgtata gtcggcggct ttttggaaat tggctttgat gatgtctata acctgctgag
     1021 ccttttccgg ctgagtaccg gcatacgcca gaaacgcccc tggcagaaat ccgtttttct
     1081 gcgtcgcgtg tatgacgtac accaactgcc tgcctctgag ctggtcatgc agccagccgc
     1141 acggatattt ataaccgctg ataatcgtat ccagcaacat aagcggcaga cggtcttcga
     1201 catttttaac agtcgggccg tttgcggcga cgattatgcc tgcctgctga atcggcacac
     1261 tgtatacgtg cgtttcgtcg ctggcggaaa tttcgcgttt gacaggcacg gccggcagcg
     1321 acgtactgcc agccggcata tcgccgaaaa gttttctgac tttcgcggca gcatttttcg
     1381 cgtcaaagtc gccgtaaatc gccaaaacgc tctcgccggc cagaatatat ttcttatgat
     1441 aatcctccag cattttacgc gtggcggatt ttatgacttc ttcataaccg ctgttcggca
     1501 gtgaccaggg agtagccggc gcataaaaat cgccccgatg ctttttcatc agcagttgta
     1561 cccaattttc atcgctgcgg cgaatatcgg caatgagcag aggcttgacg gaatccagct
     1621 ccttctgcgg aaaagtcgga cgaagaacga catctgcgaa aatatccagc gccttgccga
     1681 atttatcgct catcacttcc gccgaataaa gatagctgtt atcgccggct atcgccgata
     1741 tcgttccgcc ggcggaattg aagaacgcgg caatctcgct gccggtcata tcgcctgctc
     1801 cgcgggtgga caatacgctc atcatcaacc ccaaaccgtc ggtttttgcg gtctcgttca
     1861 atatgccgcc gcggcatgca aacgccatcg ccaccagcgg tacgtcgtgt gattcgcaca
     1921 gtaccacccg caaaccgttg ggcagggtca cgatttcggc ggcagatgaa ccgccggcgg
     1981 cagcatccgc cttagcggca ttaaaaacct tccgaggaac gattcgggtc accaccatcc
     2041 tgttgaagtt gaaatatttt ctggcaacct gacgaacctg ggcggcggtg actttctgaa
     2101 ttctgtcggt ataatttttt gaaaaattca tgtcgccggt aagcagcata tcgcagccga
     2161 gcgaggaggc tatgctaaat acatcctgtt gggcatttac gtaatcggaa attttctgca
     2221 tttttgcacg gcggagttct tcggctgaaa cttttgtttt ggaaatgcgg cgaagctggt
     2281 cgattatcgc cttttcagcc ttatcctcca tgcctggcgt acagcggaac gccacggcaa
     2341 attcaccgcc gccccacgcc ggcgtatcgg accacgtctc tactgacgtc acaaatttct
     2401 ggcggaattg aacatgctca accagacggc tggctttgcc gttggaaagt atgtaactca
     2461 gtacgtcgag tgcgtataaa tccctgtcgg taatcggtac tgtctgaaag ccgataatct
     2521 cggcgacctc ctgaaactgt tcgttcgggc ggataaaacg acgaactgac gtaagcggct
     2581 cgaccggcgg cagaacggga gcgggctgac ggccagcctg gaaatccccg aattttttaa
     2641 tcacatcagc cacggctttt gcggggtcga tatcacccgc caccacaaaa accatgtttt
     2701 gaggcacata cattcgggca tgatatgcca gaacgtcttc gtacgacacc gccgccagag
     2761 gcgcaagata accgatagtc ggcacggctg cgggatgacg gccataaaaa tccgcttcat
     2821 gcgtgtaaaa caacacgcgg tcagggtcgt caaggcccat ttccagttca cgctggacta
     2881 cgccgtgttc gcgttcaaaa tccttgcggg ttatattggc gtcggcgacc tgtgcggcga
     2941 tatagtcaat gcattgcgac attctgtctg acgatgccga aatatagtaa cacgtgtgat
     3001 ctttagccgt gtaggcgtta tcctggccgc cgatggcatc ggagagtttc ggtttatccg
     3061 cagaggcgcc ctgtgcgccg tcgtgattct cggcacagag gtgctcaagc agatggctca
     3121 gcccgcagcc gagatatttt ccttcataaa tgccgccagc tttgacgcat gcacacacgc
     3181 agacgacggg agcttttcgg acggctttgg cgattacgta catgccgttg ctcagccgca
     3241 ccgcctgaat atccggcgac gtaaccagat actgttctgc gacaacctta ggagcaacct
     3301 gaggctgcga tgcggcgacg tccgccggtt tcgccgccgc tatctgcgat ggctgcccgt
     3361 cgtcgcagcc tgccatgagc gtcgcgacca aaaacgtcac aacagccgcc gtctgtagaa
     3421 ttgttgcaaa tttcatcagt tcactttttc atcaagattg ctgaaatgag ccagcgttct
     3481 atcaagttcg tttttcagat gccgcacgcg gagaaggctt tttatgcggg tgacgagttc
     3541 caggcgattt acgggtttgg ttataaaatc atccgtgccg ttttccaccg cgcgttcgat
     3601 gtcgcccaat tcgttgagcg ccgttatcat gataattgga atatcgcgcg tctggggatt
     3661 agttttgagt ttgcggcaca cttcaaagcc gctcatcctc ggcatcatga cgtcgagcaa
     3721 aataatatcg ggctgatttt cgccgaacgt tttgtcgaga gcctctatgc cgtcggctgc
     3781 ggtatccacc gtacaacctt ccaaatcgtc cagatatgct attatcagtt cgcggttctg
     3841 ttcattatca tcgacaacca gtatcctgct ggccggcgtg gtttgggtca ttgtcgggtc
     3901 tcctgattta tctctatgaa tccatgtttc aaacgttata ctggtatcgt gtaccgtgaa
     3961 gtaatagagt agcttttttt cggtgaaatg gcaacagttt agcggatttc tgacgtttta
     4021 agtttttgat gccagcccgt ttgcgttttg gcgaaaaaat cgtcgccatg tttgaaaatt
     4081 gacaaaaacg aggtgatttt tgatggaata tcgcaatctc ggcaaaacgg agatacgagt
     4141 tacgccgata atttacggcg cgtgggcgat aggcggctgg atgtgggggc cgcaggacga
     4201 gtccgccgcc gtcgatgcca tacgtacttc gctggatgcg ggcataaatg ccatagacac
     4261 ggcggcggtt tacggcttcg gccgcagcga acggcttgtc gccaaagccg tcaaaggccg
     4321 acgcgacaaa actgtcatca tgaccaaata tggcctccgc tgggacgccg gcaatgtcgg
     4381 cacattgcga ttcaacacca ccgacctgca aggccaccct ctgaaagtct atcgctacgc
     4441 aggcgcaaaa agcgtcgtgg aagaatgcga acgctcactc aaaaatctcg acacggacta
     4501 tatagacgtc taccagattc actggcctga cgacaccacg cccctggaag agacattcgg
     4561 cgcaattgaa aaactcatga agcagggaaa aatccgcgcc gccggcgtaa gcaactatac
     4621 caccgaactc ctggccgacg ccaattcggt catccagctt gcatcctgcc agccgcccta
     4681 cagcatggta ttgcgaaatg cggaaaaaga tatcatcccc tggtgcgaag agaatcaggt
     4741 aggcgtggtc gtatattcgc cgctgcagcg cggccttctg acaggcaaaa ttactcccga
     4801 ttatcaattc gcacccgacg accaccggcg tggaaacgtt ttttttacgc cgcacaacat
     4861 tgcctctgta aacgcattcc ttgcgaaaat aaaacccatc gccgatgccc acaacgccac
     4921 gcttgcccag cttgtcataa actggaccgt gcaccgcaac gggataacag cagccctcgt
     4981 cggagcgcgt aatgcaaagc aggcacagga aaacgcggcg gcgctgaatt tcacgctcac
     5041 cagccaggag acgcaaacca taaataatct gcttgacgaa ttgaaaatcg aaaaataaat
     5101 ttttactgct agagcattga aagattgaga gttcatattt ttgcgccagt gggtttgttc
//
LOCUS       DAONFI010000159         5159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19005_length_5159_cov_3.523315, whole genome shotgun sequence.
ACCESSION   DAONFI010000159 DAONFI010000000
VERSION     DAONFI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19005_length_5159_cov_3.523315"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLK08_06140"
     CDS             complement(<1..220)
                     /locus_tag="PLK08_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53112.1"
                     /translation="MSATIDADRLTQRHRKIRTAMHPHCIVCGTDNENGGLGLEFSLC
                     DDGSVEAVFPCHERFQGYRDILHGGVTSA"
     gene            complement(204..542)
                     /locus_tag="PLK08_06145"
     CDS             complement(204..542)
                     /locus_tag="PLK08_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPS53113.1"
                     /translation="MKIAIPTAQGRLCMHFGHCEQFAILDVDADAKKIIDTKFITPPP
                     HEPGLLPRWLHELGVNVIIAGGMGQRAQVIFAQNGIKVLVGAGDATPDELALAYLNDA
                     LKVGENVCDH"
     gene            complement(539..1396)
                     /locus_tag="PLK08_06150"
     CDS             complement(539..1396)
                     /locus_tag="PLK08_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPS53114.1"
                     /translation="MSETECKSYSADESQRQLEEVELQTNMDRIGHKIMVLSGKGGVG
                     KSTVAANLAMSLAKAGKKVGLLDVDLHGPSIQKILGLDGQRAGCQQNGKIMPIRLSCN
                     FCVITLGLFLDSEKDAVIWRGPMKYNVIRQFLKDTQWGDLDYLVIDLPPGTGDEPLNV
                     AQLIGAGTTAVVVTTPQEVAISDVRRSLSFCKSLKVKVLGIIENMSGFTCPHCGRHVD
                     IFRSGGGERLAAEMAVPFLGKIPFDPQIVESGDCGKPFVEKFADSQAARAFAEIIKPI
                     IDDKTENEK"
     gene            complement(1463..1825)
                     /locus_tag="PLK08_06155"
     CDS             complement(1463..1825)
                     /locus_tag="PLK08_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPS53115.1"
                     /translation="MKIAITSQGREMTDAVDQRFGRAKFFIVADTRSGETSAVDNMQN
                     LNAAQGAGIQAGANVVSLGVEAVITGHVGPKAFATLNAGGVKIYTGAGGGVADALEKF
                     KAGELTQIQSADVESHWV"
     gene            complement(1991..3352)
                     /locus_tag="PLK08_06160"
     CDS             complement(1991..3352)
                     /locus_tag="PLK08_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPS53116.1"
                     /translation="MKPIKNIEREAAILDSVNEGVFTVDSEWRISAFNKAAERITGVS
                     RRQAIGSRCCDVFRANICETDCALRRTMQSGQPVVNTTAHIVDINGRRIPIRISTALL
                     RNNDGDIVGGVETFQDLTQVEQLQKELQSRYTFEDIVGRSPAMMRLFEIVPQIAASGS
                     TVLIEGPSGTGKELFARAIHNLSPRKKKPFIAVNCAALPDTLLESELFGHKAGAFTDA
                     RRDKPGRFAIADGGTIFLDEIGDISQAMQVRLLRVLQERVIEPLGGVKPLAVNVRVIA
                     ATNKNLSAMVQAGTFREDLYYRIRVIHLQLPSLKQRREDIPLLVEYLINRFNRLQGRD
                     IAGVSEQVMARLMEHDYPGNVRELENIIEQAFVLCRGGIIEINHLPPELRPTAGRGIA
                     ENQPMCLGDIEKLFITETLQRRGGNRKKTAADLGINVSTLYRKIRQYGIGIPEADGRG
                     QRK"
     gene            3526..3918
                     /locus_tag="PLK08_06165"
     CDS             3526..3918
                     /locus_tag="PLK08_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPS53117.1"
                     /translation="MTRVAIPHWQGRVSPVFDVAANVLIVEYDGGMERTRQDVPLETD
                     MPHARSMKLSDANVDVLICGAISKPFQAAVLTRGIEVIPQICGNVECVLSAFVEGRLQ
                     QGEFFMPGCCQRRRQREGQKRRCRQNLN"
     gene            3973..4392
                     /locus_tag="PLK08_06170"
     CDS             3973..4392
                     /locus_tag="PLK08_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HPS53118.1"
                     /translation="MANSAILDSKVVTAGKVPAGRNVVFGWELILDMYDCDVEAISTK
                     ESLARFTLELCKVIDMTPYGEPQMPYFGENCEHTKGYSLIQFIETSSITGHFSEATKT
                     AYINIFTCKAFDEKKAEEFTIKFFKAKTWVSRFMTRA"
     gene            4426..4770
                     /locus_tag="PLK08_06175"
     CDS             4426..4770
                     /locus_tag="PLK08_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPS53119.1"
                     /translation="MPRGDRTGPNGMGPMTGRGAGYCAGYSTPGYMNNGIGRCFAHFG
                     RGLGLGNGRGWRNIFRATGLTGRQRAGITNPDVSSQNGDLDALKQRAESLGENLREIN
                     RRIEELQNVSTK"
     gene            4791..>5159
                     /locus_tag="PLK08_06180"
     CDS             4791..>5159
                     /locus_tag="PLK08_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPS53120.1"
                     /translation="MKLSVASGKGGTGKTTVATNLAYVAAALGRHVAYLDCDVEEPNG
                     DLFLKPHFTEKKAVKIGVPAVDESLCTGCGQCGKICQYSAILAMNRTVLTFPELCHSC
                     GGCWEVCPSGAIKESFRQTGW"
BASE COUNT         1215 a         1405 c         1306 g         1233 t
ORIGIN      
        1 atgccgacgt cacgccgccg tgcagaatat cgcgataacc ctgaaatctc tcgtggcagg
       61 gaaagacggc ttccacgctg ccgtcgtcgc acagcgaaaa ttccaggccc aggccgccgt
      121 tttcattatc cgtgccgcac acgatacaat gcgggtgcat cgccgtcctg attttcctat
      181 gccgctgcgt caacctgtcg gcatcaatgg tcgcagacat tttcgccgac tttcagtgcg
      241 tcgttcagat atgcaagtgc aagttcgtcc ggcgtagcat cgcctgcgcc gaccagtacc
      301 ttgattccgt tctgtgcaaa aataacctga gcccgctggc ccattccgcc ggctatgatt
      361 acgttgacgc ccagttcatg cagccatcgc ggcagaaggc cgggctcatg cggcggcggg
      421 gtaatgaatt tcgtgtcgat aatttttttg gcatccgcgt caacgtcaag aatggcgaac
      481 tgttcacaat ggccgaaatg catgcacagc cggccctgcg cggttggaat tgcaatcttc
      541 atttttcatt ctccgttttg tcgtcaataa taggtttgat gatttcagca aaagcccgag
      601 ccgcctggct gtcggcgaat ttttccacaa aaggctttcc gcagtcgccc gattccacta
      661 tctgcgggtc gaacgggatt ttgccgagga acggcacagc catctcggcg gctaaccttt
      721 ctccgccgcc cgacctgaaa atatccacat gccggccgca atgcggacac gtgaaaccgc
      781 tcatgttttc gataatgccc agcactttca ctttcagcga tttgcagaac gaaagactgc
      841 ggcggacgtc ggatatggcg acctcctgcg gcgtagtgac cacgacagcc gtcgtgcctg
      901 caccgataag ctgtgccaca ttcagcggct catcgccagt gccgggcggc aaatctatca
      961 ccagataatc cagatcgccc cattgcgtgt ctttcagaaa ttgccttatg acgttatatt
     1021 tcatgggccc gcgccagatg acggcgtcct tttcgctatc caggaaaagc cccagcgtaa
     1081 tgacgcagaa attgcatgac aggcgaatcg gcatgatttt gccgttctgc tggcagccgg
     1141 ctcgttgacc gtcaaggccc aatatcttct gaatgctcgg cccgtgaagg tcaacatcca
     1201 gaaggccgac ttttttgccg gctttcgcca gagacatcgc cagattagcc gccaccgtgc
     1261 ttttgcccac tccgcccttg cctgaaagca ccatgatttt gtggccgatg cggtccatgt
     1321 tcgtctgaag ttccacttcc tccaactgcc gctgtgattc gtcggctgaa tacgacttgc
     1381 attccgtttc gctcatttac tgtcactctc tttccatgat aattatgcac ataattggcc
     1441 aggcgaggtg tgtgtgcgtg tttcaaaccc aatggctttc cacgtcagcc gattgaatct
     1501 gcgtaagttc gccggctttg aatttttcga gtgcgtccgc gacgccgccg ccagcgccgg
     1561 tgtaaatttt tacgccgccg gcattaagcg tggcaaacgc ctttggcccg acatggccgg
     1621 taatgaccgc ctccacgccg agcgaaacga cattcgcgcc agcctgtata cctgcgccct
     1681 gggcggcatt gaggttctgc atgttgtcaa cggctgaagt ttctccactc cgggtatcag
     1741 cgacaataaa aaacttcgca cgtccgaatc gctggtcgac ggcgtctgtc atttccctgc
     1801 cctgcgatgt aattgctatt ttcattttcc aaaattcctt tcgtttcata aaacctgccc
     1861 ggaaaacaaa cagcaacata catgccacaa tttccaaaaa aatcataaga cccacattta
     1921 aaaagcctta aaaacatcgt cggtactgcc atgaaatttc ggcggcgtca tattattgca
     1981 ttttgcacga ttattttcgt tggccgcggc catccgcctc aggaattccg ataccgtact
     2041 gacgtatttt tctatacaag gtgctgacat ttatgccgag gtctgcggcg gtttttttgc
     2101 ggttgccgcc gcgtcgctgc agcgtctcgg taatgaaaag tttttcgata tcgccgaggc
     2161 acatgggctg gttttccgca atgccacggc cagccgtcgg ccgtaattct ggcggcagat
     2221 gattgatttc gattatgccg ccgcggcaca acacgaacgc ctgctcgata atgttttcca
     2281 gctcgcgaac gtttcctgga taatcatgct ccatcagcct ggccatcacc tgctcgctga
     2341 cgcctgcgat atccctgccc tgcagccggt tgaacctgtt tatcagatat tccaccaata
     2401 gcggaatatc ctcgcgacgc tgtttcaggc tcggcagttg caggtgtatg acgcgaatgc
     2461 ggtaatacaa atcctcgcgg aacgtgccag cctgcaccat tgccgataga tttttattcg
     2521 tcgcagcaat cacccgtacg ttcacagcca gcggctttac gccgcctagc ggttcgatga
     2581 cccgctcctg gagcaccctc aacagccgca cctgcatagc ctgcgaaata tcgccgattt
     2641 catccaggaa aatcgtaccg ccgtcggcga tggcgaatcg gccgggctta tcgcggcgtg
     2701 cgtcggtaaa agcgccggct ttatgtccga aaagttcgct ttccagcaga gtgtcgggca
     2761 gagcggcaca gttgacggcg atgaacggct ttttcttgcg aggcgagaga ttgtgaatcg
     2821 ccctggcgaa cagttccttg cccgttccgc tggggccttc tatcaacacg gtactgccgc
     2881 tagccgctat ctgcggcacg atttcaaaca gtcgcatcat tgccgggctg cggccgacga
     2941 tgtcttcaaa cgtataacgc gactgcaact ccttctgcaa ctgctcaacc tgcgtcaaat
     3001 cctgaaacgt ctccacgccg ccgactatat cgccgtcatt gttccgcaaa agagccgtcg
     3061 atattcgtat cggaatccgc cggccattaa tgtcgacaat gtgagcggtg gtattcacta
     3121 ccggctggcc gctctgcatc gtacgccgga gagcacaatc cgtctcgcag atattagccc
     3181 tgaaaacgtc gcaacatcgg ctgccgatag cctgccggcg ggatacgccc gtaatccgct
     3241 cggcagcctt gttaaacgcg gatatccgcc attcggagtc gacggtaaaa acaccttcat
     3301 tgacggaatc gagaatggcg gcttcgcgtt cgatgttttt gatgggtttc atatcatgaa
     3361 ggctaccaca taatcgtgca atatgcaatg attttttatg caattttaat tcattttgca
     3421 ctattatatc ctgagccggc aaatccacgg ataatcataa tgccttgaaa ttactaacgt
     3481 taaatatttt aaaaaatctg gcatacttat tgcatttaac atggcatgac tagagtcgcg
     3541 ataccacact ggcagggccg tgtcagccca gtattcgacg tagccgcaaa tgtgcttatc
     3601 gttgaatatg acggcggcat ggagcgaacc cgccaggatg ttccgttaga gacggacatg
     3661 ccgcacgcca ggtctatgaa actctcggac gccaacgttg acgttctgat atgcggtgcg
     3721 atttcaaagc ccttccaggc cgctgtcctg acgcgcggca tagaagtcat cccccagata
     3781 tgcgggaatg tggaatgtgt gttgtcagcc tttgtagaag gccggctgca gcagggtgaa
     3841 tttttcatgc ccggctgctg ccagagacga cggcagcgag aaggccaaaa acgccgctgc
     3901 agacaaaatc tgaattaaac aatcaagtga ttggatagtg gatttaataa aaatttttta
     3961 ggagaaattg aaatggctaa ttccgctatt ctggattcca aagtcgtcac cgccggcaaa
     4021 gtgcctgcgg gacgcaacgt agttttcggc tgggagctta tcctggacat gtatgactgc
     4081 gacgtcgaag cgatatcaac gaaagaatcg ctggccaggt tcacgctgga actttgcaaa
     4141 gtcatcgaca tgacgccgta cggcgagccg caaatgccgt atttcggcga aaactgcgaa
     4201 catacgaaag gttacagcct gattcaattt attgagacca gctccataac cggccatttc
     4261 tccgaagcta caaaaacggc ttacataaac atattcacct gcaaagcctt cgacgaaaag
     4321 aaggctgaag aatttacgat aaaatttttc aaggctaaaa cgtgggtctc acgcttcatg
     4381 acccgcgcgt gattgttcat attacaacag aaagagaggc atattatgcc cagaggcgac
     4441 agaacaggtc ccaatggtat ggggccaatg actggacgcg gcgcaggtta ttgcgcaggt
     4501 tattccaccc caggttacat gaataacggc atcggaagat gttttgcaca tttcggcaga
     4561 ggtctggggt tgggaaatgg ccgcggatgg cgaaatattt tcagggcaac aggcctgaca
     4621 ggccgtcagc gggccggcat tacgaacccg gatgtttcgt cgcaaaacgg cgatttggat
     4681 gcgcttaagc aacgcgcaga gtctctgggc gaaaacctca gggaaatcaa ccggcgaata
     4741 gaagaactgc aaaacgtttc cacaaaatag taatgctgga cagcaatttg atgaaattat
     4801 ccgtcgcaag tggcaaaggc ggcacgggca agacgaccgt agctaccaat ctggcgtacg
     4861 tggcggcggc attgggccgt cacgtagcat atcttgattg cgacgtggaa gaacccaacg
     4921 gcgatttatt tctaaaaccg cattttaccg aaaaaaaagc cgtgaaaatc ggcgtaccgg
     4981 cagttgatga gtcgctatgc accggctgcg gacaatgcgg taaaatctgc caatatagtg
     5041 ccattctcgc catgaacagg actgttctga cttttccgga attatgtcac agttgcggcg
     5101 gttgctggga agtgtgccca agcggcgcga ttaaggaatc tttcaggcag accggctgg
//
LOCUS       DAONFI010000160         5157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19024_length_5157_cov_4.064877, whole genome shotgun sequence.
ACCESSION   DAONFI010000160 DAONFI010000000
VERSION     DAONFI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19024_length_5157_cov_4.064877"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLK08_06185"
     CDS             <1..706
                     /locus_tag="PLK08_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPS53121.1"
                     /translation="DVFPSCGGASWKREQSLLAEWLTRLPKPCGIFAATDVRARAVLD
                     AALLANIRVPDEIAVMGVDNDELFCELCQPPLSSVELNGEWAGYESARLLDRMIAGED
                     GGKTAVFVEPTGIVERQSTDVIAVDDVDVARAVRFIHSNVGRLIGVQNVADYVAASRR
                     SLEIRFKKFLNRTVNAEIQLTRIGRAKEMLLKTDMTVSRIAELAGFGSDTYMGAVFKK
                     RFGMTPLKFRQKNKIF"
     gene            complement(730..1473)
                     /locus_tag="PLK08_06190"
     CDS             complement(730..1473)
                     /locus_tag="PLK08_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPS53122.1"
                     /translation="MNVEKIDEWLDKRKNQNPFFMFMFFESPHASYNFPPECAIRPDY
                     RKDFNYARDLSKNLPHKEVTEVFNRYVNASNHLDTQLARVLRKLEEKNLIDSSIIIIT
                     GDHGEEFQEKGLWGHGGSQFHEEQLRVPLVIHIPGHAPAVIKEKSSHLDIPATVFDAL
                     GIETPSNVYSLGSSLLHGPSRQFIVAAGWDTMVYADKDCKITFPYRGEGLHHVYSASD
                     GVIKNHDEMQKRKYPQLLKILRDMRTFLR"
     gene            complement(1470..2636)
                     /locus_tag="PLK08_06195"
     CDS             complement(1470..2636)
                     /locus_tag="PLK08_06195"
                     /inference="COORDINATES: protein motif:HMM:NF023321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HPS53123.1"
                     /translation="MKSNIRKTLDVFFLLSYFAVLVNTANFLSEVAYENFVTVMFTVA
                     VWLFYSAAYLLPAYLLSHLLCFCVMRKKDEPSRRTSKIKAGLIYSAAVLLATLVQLFI
                     ITDYTVYMAFRFHCNSFVINLLTTPGGIKSMDINSESQFLFAIKIAGVLLIQLAMLFV
                     AIFLRRFRWLAGRVNPGYVWATVLTLLAVCGLSQGIGYGMAKLRWHTDILTASQAFPL
                     YQPVTFLSIGRKLGLEKKREYEVNMHVSGNTLKYPLKPMAAAPTHKYNVIYMVAESWR
                     ADMLSPDIMPQTYAFAQKHGLLFKNHYSGGVGTRMGIFSMFYGLYGPYWFNMLHETQP
                     PVLMETLRLNDYDFYCRTSQAFTYPEFDRTVFSRIPPKDMFAISDGHPSGSETE"
     gene            complement(3077..3658)
                     /locus_tag="PLK08_06200"
     CDS             complement(3077..3658)
                     /locus_tag="PLK08_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPS53124.1"
                     /translation="MRIVCIGDSLTACGGEGGHYSDYLQAWLPNDEVINKGIGGDTLG
                     GGRKRFEKDVLQLHPDVIVIELGANDFWKASTPVSELAGFLESMVADSKTAGAEVVIA
                     SCFGSRKYDNDTPTTEIARKQKFYAQQIADFENDIVSRYGCYYVPDMQVDIRPNGKSP
                     YWDDTNHPNKAGNELVARRIFTELKKAIAAKQK"
     gene            complement(3902..>4633)
                     /locus_tag="PLK08_06205"
     CDS             complement(3902..>4633)
                     /locus_tag="PLK08_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPS53125.1"
                     /translation="GIETLGGVMTKLIERNTTIPTEKKEVFSTAADSQTEVTIHVLQG
                     EREFARDNRTLGQFNLTGIPAAPRGIPQIEVTFSIDANGILNVKAKDLGTGKEQIIEI
                     KGSSGLNSDEIDKMRHDAESHAEEDKKRRQLVDLKNQADNMAYQAEKTLKENGEKVPA
                     EDRSTIESAISALKEAAKGDDAEAIKRAMQNLEQASHKMAEAMYKSASAQQQTNSAAD
                     QQGAPASEQKKDDDVIDAEYEVKKD"
     assembly_gap    4634..4643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4644..>5157)
                     /locus_tag="PLK08_06210"
     CDS             complement(<4644..>5157)
                     /locus_tag="PLK08_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPS53126.1"
                     /translation="LDEVLIHYIADEFKRTEGIELRTDPMALQRLKEAAEKAKCELST
                     AMEANINLPFITATASGPKHLTMTITRAKFEQIAEDIFNRLVNPCKKCLADAKLSPSQ
                     IDEVILVGGSTRIPKAREIVKQIFGKEPNKSVNPDEVVAMGAAIQGAILTGEVEEQMV
                     LLDVTPLSLGV"
BASE COUNT         1170 a         1268 c         1320 g         1389 t
ORIGIN      
        1 cgacgttttt ccgtcgtgcg gaggtgcatc gtggaaacgg gaacagtcgc tgcttgcgga
       61 atggctgact cgactgccca agccgtgcgg catatttgcc gcgaccgacg ttcgtgcgcg
      121 agctgtgctt gatgcggctc ttttggcaaa tatcagagtg ccggatgaaa tagcggtaat
      181 gggagtggat aacgacgagc tgttctgcga gttgtgtcag ccgccgcttt ccagcgtcga
      241 actcaacggc gaatgggcgg gatacgaatc cgcgcgattg ctggacagga tgatagccgg
      301 cgaagacggc ggcaagacag ccgtcttcgt tgagccaacc ggcatagtcg aacgccagtc
      361 gacggatgtg atagccgtcg atgatgtcga cgtcgcacgt gcggtcagat tcatacattc
      421 caacgttggc cggctcattg gcgtgcagaa cgtggcagat tatgtggcgg catcgcgacg
      481 cagccttgaa atacgtttca aaaaattcct taaccgaacc gttaacgcgg agattcagct
      541 taccagaata gggcgggcga aggaaatgct gctgaaaaca gacatgactg tttcacgcat
      601 cgccgagtta gccggtttcg gtagcgacac atatatgggc gcagtattca agaaacgttt
      661 tggcatgacg ccgcttaagt ttcgccaaaa aaacaaaatt ttctgagtac aggcagccgc
      721 gtaaaacagt tatcgcagaa aagtcctcat gtccctgaga attttgagca gttgcggata
      781 tttccgtttt tgcatttcat cgtgattctt gataacgccg tcggatgcgg aataaacatg
      841 atgcagtcct tcgccgcgat atggaaatgt tattttgcag tctttgtcgg catagaccat
      901 ggtgtcccag cctgccgcaa cgatgaattg tctgctcggg ccgtgcaaca gactgctgcc
      961 gaggctgtaa acattgcttg gcgtttcaat gcccagggca tcgaaaactg ttgccggaat
     1021 atcaaggtgc gacgattttt ccttaatcac tgccggcgca tggccaggta tgtgtatgac
     1081 taacggcacg cgaagctgct cttcgtggaa ttgcgaaccg ccgtggcccc acaagccttt
     1141 ttcctgaaac tcttcgccgt gatcgccggt tattatgatt atcgaagaat caatgaggtt
     1201 tttctcttcc agtttccgca gcaccctggc aagctgcgta tcgagatgat ttgacgcatt
     1261 gacgtaccgg ttaaaaactt ccgtcacctc tttatggggc agattttttg acaaatcccg
     1321 cgcataattg aaatccttgc gatagtcggg acggatggcg cattccggcg ggaaattgta
     1381 gcttgcatgc ggcgattcaa aaaacatgaa catgaaaaaa ggattctgat ttttccgttt
     1441 gtccagccac tcgtcgattt tttcaacgtt cattctgtct cgctgccgct gggatggccg
     1501 tcgcttatgg caaacatatc cttcggcgga atacgggaaa aaactgtcct gtcgaattcc
     1561 ggatatgtaa atgcctggct ggtacggcaa taaaaatcgt agtcgttcag ccgcagggtt
     1621 tccatcagta cgggcggctg ggtctcgtgc agcatattga accagtacgg gccgtaaagg
     1681 ccataaaaca tggaaaaaat tcccattcgc gtccctacgc cgccggaata atgatttttg
     1741 aatagcaagc cgtgtttttg ggcaaaagca tatgtttgcg gcattatatc cggactgagc
     1801 atgtcggctc gccatgattc ggcaaccatg tatatgacgt tatacttgtg cgtcggagct
     1861 gcagccatgg gtttcagcgg atatttcaga gtattgccgc tgacatgcat attgacttca
     1921 tattcgcgtt ttttctccag gccgagtttt ctgcctatgc tgaggaacgt gaccggctga
     1981 taaagcggaa aagcctgcga tgcggttaat atatcagtat gccaacggag tttggccatg
     2041 ccgtaaccaa taccctgcga caaaccgcag acggctaaca atgtgagcac tgtagcccac
     2101 acatatccgg ggttgactct gccggctaac catctgaatc gccgcaggaa aatcgcaacg
     2161 aacaacatcg ccaactgaat cagcagaaca cctgcaattt ttattgcgaa aagaaattga
     2221 gattctgaat ttatgtccat tgactttatg ccgccgggcg tagtaagcag atttataacg
     2281 aacgagttgc agtggaaacg gaatgccatg taaaccgtgt aatcggttat gatgaacaac
     2341 tgcaccagtg tggccaatag aacggcggcg gagtaaatca ggccggcttt gattttcgat
     2401 gtccgtctcg acggttcatc ttttttacgc atgacacaga agcatagcag gtgcgacagc
     2461 agatacgccg gcagcagata cgccgcactg taaaacaacc atacagccac ggtaaacatg
     2521 acggtgacga aattttcata cgccacttca ctcaaaaagt tggccgtgtt tacgagcacc
     2581 gcaaaataac tcagcaggaa aaacacatcc agcgttttgc gaatattcga cttcatgtca
     2641 atatacctca tgtaaaaccg tccgtctcaa cattatcttg cagtcaatct gtacttgtcg
     2701 gaaatttgga ataatctttg tgggaagatg gccgacgtaa tccatacgcc gccgagattg
     2761 attgtcacga atttttacag ctatttaata tatccttcat aattatacgg tttttttaaa
     2821 aaccgtaaat gaagcatccg ctgtcaggcg gacaggcaaa cgtcttataa atacgatgct
     2881 acgggtttta accgtcgaga tttagagcgt ctgacaaaag gccactcatt cggccaacaa
     2941 tgattttatt ttgttagaag cttttagtaa ttatcccaag tatttgcgga aagtcaatct
     3001 ccgattgccc tgtgtaatgg ggcgtatggt aattacggtt tatgtacttt gccgaatgag
     3061 ggttgcgtaa caggacttat ttttgtttgg cggcgatggc ttttttcaat tcagtgaaaa
     3121 ttcggcgtgc gactaactca ttgccggcct tgtttgggtg atttgtatca tcccaataag
     3181 gtgatttgcc gttcggcctt atgtcaacct gcatgtccgg tacgtaataa cagccgtacc
     3241 gcgaaacgat atcgttttcg aaatcggcta tctgctgtgc gtagaatttc tgcttacggg
     3301 caatttctgt cgtcggcgtg tcattgtcat attttcggct gccgaaacaa ctggctatga
     3361 cgacttccgc gccggcggtt tttgaatcgg cgaccatcga ttcgagaaag cctgccaatt
     3421 ccgatacagg cgtgctcgct ttccagaaat cgtttgctcc aagttctatg actatcacgt
     3481 caggatgcag ttgcagcacg tctttctcga agcgttttcg gccgccgccc aacgtatcac
     3541 cgccgatgcc tttgttgatg acttcatcat tcggcagcca tgcctgaaga taatcgctat
     3601 aatggccgcc ttcgccgcca catgcggtaa gactgtcgcc gatacatact atacgcatag
     3661 gcctgttatc tccgcacccc gaaaagaaaa ttccgcccgc caataacaat attgcaacca
     3721 atcgcataaa taaatacctc ctgcactgtt catatgcagc ctttcatgct ctagcgtttt
     3781 taaattcaaa aactacttta atttgtcaga tacgcatttg caagccgctg gcgcataaaa
     3841 aagcgtagcc gcacatcggg ctacgctttt tgttcattcc gttaataacc ggacagatac
     3901 gtcaatcctt tttgacttcg tattctgcgt cgattacatc atcgtctttt ttctgttcac
     3961 ttgcaggtgc accctgctgg tcggcggcgg agttcgtctg ctgctgtgca gaagcggatt
     4021 tgtacatagc ctccgccatc ttgtgcgacg cctgctcaag attctgcatc gcgcgtttga
     4081 ttgcctctgc gtcgtcgcct tttgcagcct ctttcagggc gctgattgcc gactcgatag
     4141 tgctgcggtc ttcagccggc actttttcgc cgttttcctt gagggtcttt tccgcctggt
     4201 aagccatgtt atctgcctgg tttttcaggt cgactagctg gcgccgtttt ttatcctctt
     4261 cggcgtgcga ttcggcatca tggcgcattt tatcgatttc gtcgctgttc aggccgcttg
     4321 agcctttaat ctcgatgatc tgttccttgc ctgtgccgag gtctttggcc ttgacgttca
     4381 atatgccgtt ggcgtcaatc gagaatgtga cttcaatctg cggtatgcct cgcggggcag
     4441 ccggtatgcc ggtcaggttg aactggccga gcgtacggtt gtcgcgggca aattcccgct
     4501 cgccctgcag cacgtgaatg gtcacttcgg tctggctgtc tgcggcggta gagaagactt
     4561 ccttcttttc agtcggaatg gtcgtgttgc gttcgatgag cttggtcatc acgccgccaa
     4621 gggtttcgat gccnnnnnnn nnnaacgccc aatgacagcg gggtaacgtc aagcagcacc
     4681 atctgctctt cgacttcgcc ggtgaggatt gcgccctgta ttgcggcgcc catagccacc
     4741 acttcgtcgg ggttgaccga cttgttgggc tctttgccga aaatctgttt gacgatttcg
     4801 cgggctttgg ggatacgggt cgaaccgccg acgagtatga cttcgtcaat ctggctgggc
     4861 gagagtttgg cgtcagccag gcattttttg cacgggttga caaggcggtt gaatatgtct
     4921 tcggcaatct gttcgaactt ggctcgcgtg atggtcatgg tcaggtgttt ggggcccgac
     4981 gcggtagcgg tgatgaacgg cagattgatg ttcgcctcca tcgcggtgga cagttcgcac
     5041 ttggcctttt ccgcagcttc cttcaggcgc tgaagcgcca tcgggtctgt gcggagttcg
     5101 atgccctcgg tgcgtttgaa ctcgtcggcg atgtagtgaa tcagcacttc gtccagg
//
LOCUS       DAONFI010000161         5154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19039_length_5154_cov_4.667582, whole genome shotgun sequence.
ACCESSION   DAONFI010000161 DAONFI010000000
VERSION     DAONFI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19039_length_5154_cov_4.667582"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLK08_06215"
     CDS             complement(<1..613)
                     /locus_tag="PLK08_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53127.1"
                     /translation="MPAFPVKITNLLITAVLCAATLLAGCSDADRKTSQHSRTDAEFF
                     AGIEKKWGCPFSQLPSEKLVIISPHNQSIQEEFSRAFSHKYALDNGRRVECEWRDVGG
                     GGNKILSYLQNVYSRASGSGIDVVWGGGMDIFNTLRDGGLLQKLVLPATVLENIPQYV
                     AGGEFRDPQGYWCGSTVSNFGILANLPLLKRLGRPVPHTFRDLG"
     gene            772..1884
                     /locus_tag="PLK08_06220"
     CDS             772..1884
                     /locus_tag="PLK08_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPS53128.1"
                     /translation="MKAVVTGQVGLNKKKFIRRVHEIALEMGMDFKIFNVGDMMYAEA
                     PDVAKGKILDLPLVRLDNLRRSVFKDILAEADRGCDLLVNTHATFRWNHGLFFAFDHD
                     QMAMLDADLYIVLVENIDRVHYRLTRDGFAEHILKDLMVWREEEILTTELLSRVMRGH
                     GCFYICAQGDDSTAQTVARLMYCSREMKKAYLSFPMSHVADQPEILREIDEFRMMLKK
                     CFICFDPADIEEKQLCFMAADAARAGRNFVEVGPYDEKARLDLHQLLDIIPDIDGQIY
                     VRDFKLIDQSDMIISLVPELPGGKPAISSGVERELQHAHESTRDVFVIWKPRREPSPF
                     ITETATKVFHSTQEAMGYFLQNGYIGHNDNPGPLFG"
     gene            1932..2564
                     /locus_tag="PLK08_06225"
     CDS             1932..2564
                     /locus_tag="PLK08_06225"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HPS53129.1"
                     /translation="MKILVTAGPTREPIDSVRFITNASSGRMGIAVANAAAAAGHEVT
                     LLLGPVADDAVSKNVTVVRFVTFDDLKAAAESRFGSCDVLVMAAAVGDFTVKNRTETK
                     ISRRNGPIELELVPTPDILGELGRRKSCRQRIISFAVEDGGRQQAIVDKARSEMARKN
                     ADFCVLNTPAAMAAEASMACILSPADFVLQWAFRSKTELAEEIVRIFESE"
     gene            2579..3034
                     /gene="dut"
                     /locus_tag="PLK08_06230"
     CDS             2579..3034
                     /gene="dut"
                     /locus_tag="PLK08_06230"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPS53130.1"
                     /translation="MNCPININICRIDGHEDVPLPRYMTEHSAGMDVCAAVTQDVVLS
                     PGDIKLIPCGFTMSIPDGYEAQMRPRSGLALKHGIILPNSPGTIDADYRGPVGVILSN
                     IGSEDFVIKRGMRIAQMIIGKVEKACVVEVESLDCTSRGAGGFGHTGLH"
     gene            3128..>5154
                     /locus_tag="PLK08_06235"
     CDS             3128..>5154
                     /locus_tag="PLK08_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPS53131.1"
                     /translation="MTKRSKTTRTDDNTEKNGPRFWWYCFSLLLLGAVVLCWVSVLSF
                     VVSDSPNADYLRPDAAVQNRCGIIGAYIAYYTRYFLGVGSYATLVCLTLWSLRLMSGR
                     AVPYWTWRMTGMFVLTAGISAGAYMLQPNLATPFAETSAGILGSYTGTLLGVFGPMGQ
                     WLVLGIVLCIGLVFAADNFVAGVLAALKKLFAPKSELAIKFAELKQKRKEEKERLKAA
                     KLAAVEEARKLKEQEKRRRKEEKEQARQAAIAEKQAAKAAADENASAMQAEQQSEEAV
                     PEDIELQQDILLEDIKTSSAETSAAIPSPSGSAPLFGDIPKPQPPQKQKSAPKPKKTR
                     KTDLELPSLSLLDEPQRGYSEKAEVSALQRKITLQQTLDDFGVQANVEGYMTGPVITL
                     FEVALAPGVKVAAISNLATDIARSLAVPGVRIVPPRFGKNTVGIEVPNVDKEIVRLKE
                     LVNLQPDAPDHMELPLYLGKDAGGNPIITDLAKCPHMLIAGTTGSGKSVCINTIIMSL
                     LLTRQPEDVRFILVDPKMVEMAAFEKLPYLLCPTINDTKKAESILEWATIKMDERYEI
                     LREAGVKNLASFNELSEKEIYEAFQADTPEEQARVVTHMPSIIIIIDELADMMMTSGK
                     EVESYIIRIAQKARAVGIHLVLATQRPSANVVTGLIKSNMPCRVSFRVASG"
BASE COUNT         1231 a         1282 c         1464 g         1177 t
ORIGIN      
        1 cgcccagatc gcgaaacgta tgcgggacgg gccggccgag acgttttaac agcggcagat
       61 tcgccagtat accgaaattg ctcaccgtac tgccgcacca gtaaccttgc gggtcgcgga
      121 attctccgcc ggctacatat tgcggaatat tttcgagaac ggttgccggc agaacaagct
      181 tttgcagcag gccgccgtcg cgaagcgtat tgaaaatgtc catcccgccg ccccatacca
      241 cgtctatgcc gctgcctgac gcccgcgaat agacgttctg cagatagctt aaaattttat
      301 tgccgccgcc tcccacgtca cgccattcgc actctactcg tctgccgttg tccaacgcgt
      361 acttgtggga aaacgcgcgc gaaaattctt cctgtatgga ctgattgtgc gggctgatga
      421 ttacgagttt ttccgagggc agttgtgaaa atgggcagcc ccattttttt tcgattccgg
      481 caaaaaattc ggcgtcggtt cgactgtgtt gcgacgtttt tctgtcggcg tcggagcagc
      541 cggccagcag cgtcgcggca cacaatacag ccgtgataag taaattcgtt atttttaccg
      601 gaaaagcagg catgacatga gtttattgaa tttgcgggaa attcgcaata aaaatgaacg
      661 gctggacttc tggtcgagac tgtcttgttg ccgccgcggc tgggtacttt tctgtggcac
      721 ttttgcggtg catgatatag actgtcgctc tgtatcatca ggagttttca aatgaaagca
      781 gtggttaccg gacaggtcgg cctgaacaag aagaaattca tccggcgggt gcatgaaatc
      841 gccctcgaaa tgggtatgga tttcaaaatt ttcaacgtcg gcgacatgat gtacgccgag
      901 gcgccggatg tggcgaaagg caagattctc gacctgccgc tggtgcggct ggacaacctt
      961 cgtcgcagcg tttttaagga tattctcgcc gaagccgacc gcggctgtga cctgttggtg
     1021 aacactcatg ccacgtttcg ctggaatcat gggctgtttt tcgcgttcga ccacgaccag
     1081 atggctatgc tggatgcgga tttgtacata gtgcttgtgg aaaacatcga ccgcgtgcac
     1141 tatcggctga ctcgcgacgg ctttgcagag catatcctca aagatctcat ggtctggcgc
     1201 gaggaggaaa ttctgacgac ggaactgttg tctcgcgtaa tgcgcggcca cggctgtttt
     1261 tacatttgtg cgcagggtga tgattcgact gcgcagacgg ttgcaaggct gatgtattgc
     1321 agccgtgaga tgaaaaaggc gtacctgagc ttcccaatgt cgcacgtggc tgaccagccg
     1381 gaaattttac gtgaaatcga cgaattcaga atgatgctta aaaaatgttt catatgtttc
     1441 gaccctgcgg atatcgagga aaaacagctg tgcttcatgg ctgccgacgc ggcgagggcg
     1501 ggacggaatt tcgtcgaggt tggcccttac gatgaaaagg ccaggctgga tttgcaccag
     1561 ttgctggaca tcattcccga catagacggc cagatttacg tgcgggattt caaactgata
     1621 gatcagtccg acatgataat ttcacttgtg ccggaactgc cgggcggcaa gccggctatt
     1681 tccagcggcg tggaacgcga acttcagcat gcccatgaat ccacgcgcga cgtgttcgta
     1741 atctggaagc cgcgccgtga gccgagtccg ttcataactg aaacggctac aaaggttttc
     1801 cattcgacgc aggaggccat gggctatttt ttgcaaaatg gctacatagg ccataatgac
     1861 aaccccggcc cgctcttcgg gtaaaaatcg caaattgaaa tttttacctt tttgcggtat
     1921 catcttcacc catgaaaata cttgtgacag ccgggccgac gcgcgagccg atagattccg
     1981 tgcgattcat aaccaatgca tccagcggcc gaatgggcat tgccgttgcc aatgcggcag
     2041 cggcggctgg ccatgaggtg acgctgctgc tggggccggt ggcggacgat gctgtctcta
     2101 aaaatgtaac tgttgtcaga ttcgtgactt ttgacgattt gaaggctgct gccgagtcgc
     2161 ggtttggcag ctgtgacgta ctggtgatgg ctgccgccgt tggggatttt accgtcaaaa
     2221 atcgaacgga aaccaaaatt tcacgtcgaa acggcccgat tgaacttgag cttgttccga
     2281 cgccggatat tctcggcgaa ctcggcagac gtaagtcctg tcgtcagcgg ataatatctt
     2341 ttgccgtgga agacggcggc cggcagcagg ctatcgtcga taaggctcgc agcgagatgg
     2401 ctcgcaaaaa cgccgatttc tgtgtactga atacgcctgc ggccatggcg gctgaggcga
     2461 gcatggcctg catactttcg ccggctgatt ttgtgctgca atgggcattc cgaagcaaaa
     2521 ccgaactggc tgaagaaata gtcagaattt ttgagtccga gtgactggag agatgtgtat
     2581 gaattgtccg ataaacataa atatatgccg tatagacggc catgaagacg taccgctgcc
     2641 gagatatatg accgagcatt ccgccggcat ggatgtctgt gccgcggtta cccaggatgt
     2701 cgtactctcg ccaggtgata taaagctcat cccgtgcggt tttacgatgt ccattcccga
     2761 tggttacgag gcgcagatgc ggcctcgcag cgggctggct ctcaagcacg gtataattct
     2821 gccgaattcg ccaggtacga tagatgccga ttatcgcggc ccggtaggcg tgattttgag
     2881 caacatcggc agtgaagatt tcgtaataaa acgcggtatg cgtatcgcgc agatgataat
     2941 aggcaaagtt gaaaaagcct gcgtagttga agtagaatcg ctggattgca cttcacgcgg
     3001 cgcgggcggt ttcgggcata ccggcttgca ttgacgttgt gggattcgca gttgtcggct
     3061 gagcgttaca gccgacaatc ccgtaaaatc tcaatgtcgc cgataaatgc ccgcaaaaga
     3121 tacacggatg actaaaaggt ctaaaacaac acggacggat gacaacactg aaaaaaacgg
     3181 accgcggttc tggtggtact gtttttcact ccttctgctc ggcgctgtag tgctatgctg
     3241 ggtcagcgtg ctgagttttg tggtctccga ttcgccgaat gcggattatc tgcggcctga
     3301 tgccgcggtg cagaaccgtt gcggcataat cggcgcgtat atcgcctatt acacgcggta
     3361 ttttctgggc gtcggctcat atgcaacgct ggtctgtctg acgttatggt ctctgcggct
     3421 gatgagcggc cgggctgtgc cgtattggac gtggcgaatg accggcatgt tcgtattgac
     3481 ggctggcata agcgcggggg cgtatatgct tcagccgaat ctcgcaacac cctttgccga
     3541 aacgtccgca ggaatactgg gttcgtatac gggtacgctg ctgggcgttt tcgggcctat
     3601 gggccaatgg ctagtattgg gtatagtcct gtgcataggc ctggtttttg cggctgataa
     3661 cttcgtcgca ggcgtattgg cggcgctgaa aaaacttttt gcaccgaaga gcgaacttgc
     3721 aattaaattc gccgaactca agcagaaacg caaggaagag aaggaacgtc tcaaagccgc
     3781 aaaactggcg gctgtcgaag aggctcgtaa actcaaggaa caggaaaaac ggcgaagaaa
     3841 agaggaaaag gagcaagctc gccaggcggc gattgccgaa aaacaggcag ctaaggctgc
     3901 agccgatgaa aatgcctccg ccatgcaggc cgagcagcag agtgaagaag ctgtgcctga
     3961 agatattgaa ctgcagcagg atattttgtt ggaagatata aaaacttctt ctgcggaaac
     4021 atccgctgcg attccttcgc cttcaggttc agccccgctg tttggcgaca tacccaaacc
     4081 tcagccgccg caaaaacaga aatctgcacc gaaacccaaa aagacccgta aaaccgacct
     4141 ggaactgccg tcgctttcat tgcttgacga gccgcagcgc ggctattccg aaaaggctga
     4201 agtttccgcg ctgcagcgta aaatcacatt gcagcagacg ctggacgatt ttggcgtgca
     4261 ggcgaatgtt gagggttaca tgacagggcc ggtaatcacg ctgttcgagg tggctcttgc
     4321 gcctggcgtg aaagtggcgg cgatcagtaa tctggcgact gacattgcgc gttcgctggc
     4381 tgttccgggc gtgcgaatag tgccgccgag attcggcaaa aacacggtcg gcatagaagt
     4441 gcccaacgtc gataaggaaa tcgtccgcct gaaagaactc gtaaatcttc agcccgatgc
     4501 accggaccat atggaactgc cgctgtacct gggcaaggat gcgggcggca atccgattat
     4561 caccgatttg gccaaatgcc cgcatatgct catcgccggt acgacaggtt ccggcaagag
     4621 cgtgtgcata aacaccataa taatgtcgct gctgctgacc cgtcagcccg aagacgtgcg
     4681 attcatactc gtcgacccga aaatggtgga aatggcggcg tttgaaaaac tgccgtacct
     4741 gctctgtccg actatcaacg ataccaagaa ggcggaaagc atacttgagt gggctaccat
     4801 aaaaatggac gaacgctacg aaatccttcg tgaagccggc gttaaaaatc tggcgtcgtt
     4861 caacgagctg tcggagaagg aaatttacga ggcgtttcag gctgatacgc ctgaggaaca
     4921 ggcgagagtc gtcacgcata tgccgtcgat aatcataatc atcgacgaat tagccgacat
     4981 gatgatgacg tccggcaagg aagttgaatc atacataatc cgcattgcgc agaaagcccg
     5041 cgcggtcggc atacacttgg tgcttgcgac gcagcggccg agtgcgaacg tagtcacggg
     5101 ccttattaaa tccaatatgc cctgcagggt gagtttccgc gtagcctccg gcca
//
LOCUS       DAONFI010000162         5152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19050_length_5152_cov_4.025113, whole genome shotgun sequence.
ACCESSION   DAONFI010000162 DAONFI010000000
VERSION     DAONFI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19050_length_5152_cov_4.025113"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..2981
                     /locus_tag="PLK08_06240"
     CDS             393..2981
                     /locus_tag="PLK08_06240"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014028255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HPS53132.1"
                     /translation="MSDNFKGDVISLTDEKVQNSSIGDIFAVLRSSADGITLQEAKDR
                     LAIFGRNELTEKKESSLLKFLLYFWGPIPWMIEAAAILSLVVGDYRDFAIIMAMLIFN
                     ALIGFWEEHKASNALAALKAGLALKARALRDGKWGEVPAAQLVPGDVIHIRLGDIVPA
                     DVKLFDGQYASIDQAALTGESLPVNKKIGDSAYSGSVVKQGEMLAVVTATGENTFFGR
                     TAKLVEDAGTVSHFQQAVMRVGDFLIFLSIGLCLLLATVVIYRDLAAGPAGLTLHNVL
                     SLVKFMLILAVASIPVAMPAVLSVTMALGALALSKQKAIVSKLQSIEEMAGVDILCSD
                     KTGTLTKNQLTLGDVTLFAASDPQQCILIASLASKEENQDAIDTAIITSLADINVLKE
                     YRQTNFVPFDPISKRTESTVIGPAGAKRCTKGAPQVIMDMCKLGDAEKERAAATVKEL
                     AGRGYRALGVAQSSNDGETWSFLGILSLFDPPRDDSAETIKQAGQHGIKVKMVTGDDT
                     AIAAEISGKLGMGAHIQPANTLFTEGTDPNLLNPQQALAIERADGFARVFPEHKYGIV
                     KGLQSRGHIVAMTGDGVNDAPALKQADCGVAVSGATDAARAAAALVLTMPGLGVIINA
                     VDTARKIFSRMMSYTIYRIAMTMDVMFFVVLSMLIFPQFQPLTPIMIVILALLDDIPI
                     MTIAYDNANSSPKPQKWLMGRTLTISSVLGVLAVVETFGLLLWGMKNIGHDFFNVIID
                     PTHLQTMAFLQLVVGGHLMLFVTRTKRPILMPSYPSLPLFAAIIGTQIFAAVLCGIGW
                     NSVIPRLPWQLIGLVWVYNLVWMFILDIAKLATYALMDNNTRNRKWTSRAISLQLQPH
                     GSLHVK"
     gene            3150..>5152
                     /locus_tag="PLK08_06245"
     CDS             3150..>5152
                     /locus_tag="PLK08_06245"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPS53133.1"
                     /translation="MDRPGKKFKRVLLACRESGDIEPIVKRLSLIFPDHKFSMVTKGK
                     DVFNAINRKEVDILVIADKLKDTTAEVVCKHLKSHEETSGIYTIAFGPLANDVFCSNI
                     LKAGADDILRKPFFLSELIARLKQAFRTLHLLNAVRDKHVSISGRTGDTSINQINTLM
                     DSHDNAMLIIALDGSILFYNPAIAYRLGYTDEELHHLSARDIIAESDSRHGMAMLDDI
                     LAGKTKACTIPLQTSKKEILQVELEVVRGRWHNQDVFFGIAKNIVAEQKIHQTLRKRE
                     DVLEAVSYSANHLLGNFEWSQCLMDVLGHLGEATGAVRAYICENTRTLNTVSGYKILH
                     EWTACGTPSKFDGDLRLMKYEDCFNEYIGLLSADQPVTIHCKKLSNNLMFHKFMQNLA
                     SVILIPIFTGDDWWGYLGFDYADSTDVLSDSELEALCTAARIFGTGLHRQQVQLSMQG
                     NLQFLETFLDTIPSPAFYQDVKGVYRGCNAAFSQLIIGKSKREILNHCIFDFPKSFPP
                     ALAEYFHQKDLDLLEKPGYQTFEATFECSDNIQRTFLFDKTSFLDDQSRVIGICGVMT
                     DITELKTAESELNTARQREINIGFEIQNRLLLTPPPQNLCGAEVAAISIPSHQIDGDF
                     YDFIRYDSTHFDVFIGDVMGKGVSAAMLGAATKTQFLRSVTNLV"
BASE COUNT         1345 a         1340 c         1276 g         1191 t
ORIGIN      
        1 ccagccgacg ccgcaaacag caacaagcgc aaagtcaagt ttaatatgct ctagtaccca
       61 aattccggaa tttcaacagg ccagacttct tgaagttcgc atgcgcatgt attttctttt
      121 tttatttcgt ctgcaacctt gaaaccgggg gctggctgac tatactttaa tatatctcaa
      181 tgtggttgat ttgtgatatt gcgttcgtta aaaagctatg ttgcatacag ttacggtttt
      241 gacgaataac gggtttctga cccgttattc aatcgcacag cttattgcaa ttaaaccgtt
      301 aaattttgtg catcgcgcgg tatggacgta tttcaaatcg gtcggattga atgatatata
      361 aggttttggc aaaaaatctt aggaggacgc ccatgtcgga taatttcaaa ggagacgtta
      421 tctcgctgac ggacgaaaag gtacagaatt ccagcatcgg agatattttt gccgttctgc
      481 gttcatcagc cgacggtatt actttgcaag aggcgaaaga ccggctggcg atattcggac
      541 gcaatgagct gacggaaaaa aaggaatcgt cgctgctgaa attcctgctc tatttctggg
      601 ggccaatccc ctggatgata gaagctgccg ccatcctctc cctcgtagtc ggcgattaca
      661 gggattttgc cataatcatg gctatgttga tttttaatgc cctgataggt ttctgggagg
      721 aacacaaggc gtccaacgcg ctggctgcgc tcaaggcggg actggcgtta aaggcgcgtg
      781 cactgcgtga cggcaaatgg ggcgaagtgc cagccgcgca actcgtacca ggcgacgtca
      841 tacacatacg gctgggcgac atagtgccag ccgacgtaaa actcttcgac ggccaatatg
      901 caagcataga ccaagccgcc ctaaccggcg aatcactgcc ggtaaacaaa aaaatcggag
      961 attccgccta ttccggctcg gtcgtaaaac agggagaaat gctcgccgtc gttacggcaa
     1021 ctggcgaaaa cacttttttc ggacgaactg caaaactggt ggaagacgcg ggcacagtca
     1081 gccatttcca gcaggcagtc atgcgcgtag gcgattttct gatatttctc tcgataggcc
     1141 tctgccttct gctggcgacg gtggtgattt atcgcgacct tgcagctggg ccagccggct
     1201 tgacgctgca taacgtactg tcgctggtca aattcatgct catcctggca gtagcgtcga
     1261 ttccggtggc aatgccggcg gtgctttccg taaccatggc cctgggcgca cttgcacttt
     1321 caaaacagaa ggcaatcgta tccaagctgc aatccatcga agagatggcc ggcgtggata
     1381 tactatgcag cgacaagaca ggcactctta ccaaaaatca gcttacgctc ggcgacgtca
     1441 cgctttttgc cgcctccgac ccgcagcagt gcatactcat cgccagcctc gccagcaagg
     1501 aagaaaatca ggacgccata gacacggcga taatcaccag tctggcggat atcaacgtac
     1561 tcaaagaata ccggcagaca aatttcgtac cgttcgaccc aatcagcaag cgtacggaat
     1621 ccacggtaat cggcccagcc ggtgcaaaac gttgcaccaa aggcgcgccg caggtgatta
     1681 tggatatgtg caaacttggc gatgccgaaa aagagcgggc agccgcaacg gtcaaagaac
     1741 tcgccggccg aggttaccgc gcattgggag tggctcagag ttccaacgac ggcgaaacat
     1801 ggagcttttt gggaattcta tccctgttcg acccgccgcg cgacgattct gcggaaacaa
     1861 taaagcaggc tggccagcac ggcataaaag taaaaatggt caccggcgac gacaccgcca
     1921 tagccgctga aatatccggc aaactgggca tgggcgcaca cattcagccg gcaaatacgc
     1981 tctttaccga aggcacggac ccgaatttgc tcaacccgca gcaggcgtta gccatcgaac
     2041 gggctgacgg ctttgccagg gtttttccgg agcacaagta tggaatcgtc aaagggctgc
     2101 aatcccgcgg ccatatagtg gcgatgacag gcgacggcgt aaatgacgct ccggcgttga
     2161 aacaggcgga ctgcggcgta gccgtttccg gcgcaacaga cgccgcacga gccgccgcgg
     2221 ctctcgtact taccatgccg ggactcggcg taatcatcaa cgccgtcgat acagcccgaa
     2281 aaatcttctc aagaatgatg agctatacca tatatcgcat agcaatgacc atggacgtga
     2341 tgttcttcgt ggtgctgtca atgctgattt tcccgcaatt ccagccgctc acgcccatta
     2401 tgattgtaat tcttgcactg ctggacgaca taccgattat gaccatcgca tacgataacg
     2461 ccaacagcag cccaaaacct caaaaatggc tgatgggcag aactcttacg atttcatcgg
     2521 tactgggcgt gttagccgta gtagagacat ttggtttgct gctctggggc atgaaaaaca
     2581 tcggccacga ttttttcaac gtgataatag atccgacgca cctgcaaaca atggcttttc
     2641 tgcagcttgt cgtgggcggc catctgatgc tgttcgtaac caggacgaaa cggccaatac
     2701 tgatgccgtc atatccgtca ctgccgcttt tcgcggcgat tatcggcact cagatattcg
     2761 ccgccgtctt gtgcggcatc ggctggaaca gcgtcatacc gcggctgccg tggcagctta
     2821 tagggcttgt gtgggtatat aacctcgtgt ggatgttcat actggatatt gccaaactgg
     2881 ctacctatgc actcatggac aacaatacca gaaaccgaaa gtggaccagt cgtgcaattt
     2941 cgctgcaact gcagccgcac ggctctttgc acgttaaatg aaaaccgata atttccgaaa
     3001 tataaatttt tatataaacg gaatttttta gagcggctct caaaagaaat ttattttgag
     3061 aatcgtgcta aaatattccc ttaaaaacgt gtagtgtata taatctacaa atatggaagc
     3121 aacttccgct ttttattgcg aggatttttt tggacagacc tggtaaaaag tttaagcgag
     3181 tcctgctggc atgtagggaa agcggtgaca tagagcctat cgtcaaacgg ctgtcgctga
     3241 tatttcccga ccacaagttc agtatggtta ccaagggcaa ggacgtattt aacgccataa
     3301 accgcaagga agtcgacatc ctcgtaatag ccgacaaact caaagacacc accgcggaag
     3361 ttgtctgcaa acatctgaaa tcgcatgaag agacgtcggg tatttacacc atagctttcg
     3421 gcccccttgc caatgacgta ttctgcagca acatcctgaa agccggcgca gacgacattc
     3481 tccgtaagcc gtttttcctg tccgaactca tcgctcgcct caagcaggcg ttccgtacgc
     3541 ttcacctgct gaatgccgtc agagacaaac acgtttccat ttccggccgt accggcgaca
     3601 cctccataaa ccagataaat accctcatgg attcgcatga caacgccatg ttgataatcg
     3661 cacttgacgg cagcatactt ttttacaatc cagccatcgc ataccggttg ggctataccg
     3721 atgaagagct gcatcatctc agtgccaggg atatcatcgc cgaatccgac agccgccacg
     3781 gcatggccat gcttgacgac attctagccg gcaagaccaa agcctgcaca atcccgctac
     3841 agaccagtaa aaaggaaata ttgcaggtgg aactggaggt cgttcgcggc agatggcata
     3901 atcaggatgt atttttcggc atagcaaaaa acattgtggc ggaacagaag atacatcaga
     3961 ccctccgcaa acgggaagac gtgctggagg cggtaagtta ttcagccaac catcttctgg
     4021 gcaatttcga atggtcgcaa tgcctgatgg acgttcttgg ccacctcggc gaagcaactg
     4081 gcgcagtacg ggcatacata tgtgaaaaca cccgcactct taacactgtt tcagggtata
     4141 aaatcctgca cgaatggaca gcctgcggca cgccgtcaaa atttgacggt gacctgcgat
     4201 taatgaaata tgaggactgt ttcaatgaat atatcggcct gctctcagcc gaccagccgg
     4261 ttacgattca ttgcaaaaaa ctctcgaaca atctcatgtt tcacaaattc atgcagaatc
     4321 tcgcatcggt gattctgatt ccaatattca ccggcgatga ctggtgggga tacctgggct
     4381 tcgattatgc cgacagtacc gacgtattgt cggacagcga gcttgaggcg ctttgtacag
     4441 ccgccaggat tttcggcaca ggccttcacc gtcagcaggt acaactcagc atgcagggca
     4501 acctgcaatt tctcgaaacg ttcctggaca cgatacccag ccctgcattt tatcaggatg
     4561 tcaagggggt ttatcgcgga tgcaacgcgg cgttttctca acttatcata ggcaaatcga
     4621 aacgggagat tctcaaccat tgcatatttg attttcccaa aagttttccg ccggcgttgg
     4681 ctgaatattt tcatcagaaa gatctggatt tattggaaaa gcccggctat cagacatttg
     4741 aagccacctt cgaatgctcc gacaacatcc aacggacgtt ccttttcgac aagacttcgt
     4801 ttctggacga ccagagccgc gtcatcggca tatgcggcgt gatgactgac ataaccgaac
     4861 tcaagacggc tgaatcggaa ctgaacacgg cgcggcagcg tgaaataaac atcggctttg
     4921 aaattcagaa ccggctgctg cttacgccgc cgccgcagaa tctttgcggc gcagaagttg
     4981 ccgccatatc cattccgtcg caccagattg acggcgattt ttacgacttc atccggtacg
     5041 attcgacgca tttcgacgta tttatcggcg atgtcatggg caagggcgta tctgcggcga
     5101 tgctaggagc cgccaccaaa acgcaatttt tacgaagcgt caccaacctg gt
//
LOCUS       DAONFI010000163         5084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19399_length_5084_cov_4.409624, whole genome shotgun sequence.
ACCESSION   DAONFI010000163 DAONFI010000000
VERSION     DAONFI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19399_length_5084_cov_4.409624"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /gene="rpsH"
                     /locus_tag="PLK08_06250"
     CDS             complement(<1..104)
                     /gene="rpsH"
                     /locus_tag="PLK08_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPS53134.1"
                     /translation="MSLSDPIADMLTRIRNAIQAKHSVVNIRASKICEG"
     gene            complement(120..305)
                     /locus_tag="PLK08_06255"
     CDS             complement(120..305)
                     /locus_tag="PLK08_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPS53135.1"
                     /translation="MASKSKIAKAKLTPKFSSRTVRRCQLCGRSRSVYRKFRVCRLCL
                     RKMALDGLIPGMKKASW"
     gene            complement(325..861)
                     /gene="rplE"
                     /locus_tag="PLK08_06260"
     CDS             complement(325..861)
                     /gene="rplE"
                     /locus_tag="PLK08_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPS53136.1"
                     /translation="MSRLKEKYKKEIMPAFVADGIANPMSIPAVKKVVISMGLGQHST
                     DKARYEACAEQLAKLSGQKPIITKARKSVSNFKVRAGMNAGMKVTLRRERMYEFLDRL
                     ISLAIPRVKDFRGLNPSGFDGQGNYNMGLSEQSVFPEIDPAAMTFTQGMNISIETSAE
                     NDEQARKLLKLMGMPFRD"
     gene            complement(880..1209)
                     /gene="rplX"
                     /locus_tag="PLK08_06265"
     CDS             complement(880..1209)
                     /gene="rplX"
                     /locus_tag="PLK08_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPS53137.1"
                     /translation="MRVKKGDMVVVVAGDDKGKTGEVIRVYPDESRVLVKGINEVFRH
                     LRPSRKNPKGGRMKKEMPVHISNVMPIDPETSQPTRVRFAVNADGSKERLAVKSGKSL
                     GVVRKAR"
     gene            complement(1218..1607)
                     /gene="rplN"
                     /locus_tag="PLK08_06270"
     CDS             complement(1218..1607)
                     /gene="rplN"
                     /locus_tag="PLK08_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002563169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPS53138.1"
                     /translation="MIQAETRLNVADNSGARIIGCIRVLGGSTTRRHGMTRRMAGLGD
                     VIIASVKKAIPGGDVKAGDVVRCVIVRAAKKTRRKDGSYVRFDSNAAVLIDKDGNPKG
                     TRIFGAVARELREKNYMKIVSLASEVV"
     gene            complement(1626..1901)
                     /gene="rpsQ"
                     /locus_tag="PLK08_06275"
     CDS             complement(1626..1901)
                     /gene="rpsQ"
                     /locus_tag="PLK08_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPS53139.1"
                     /translation="MTNEAQKKNHNRRAKVGTVISKSGSKTVSVQIDNLVKHETYGKY
                     LRRRTKLAVHDELEQAKVGDMVEIVPVRPLSKKKAHRLVRVVRPAVG"
     gene            complement(1923..2129)
                     /gene="rpmC"
                     /locus_tag="PLK08_06280"
     CDS             complement(1923..2129)
                     /gene="rpmC"
                     /locus_tag="PLK08_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPS53140.1"
                     /translation="MKINEIKNMSDAELQAELANLKRRIFDLRSQSVTEKLENTSQIT
                     KTRKDIARISTVIRQRELSAQQTA"
     gene            complement(2160..2579)
                     /gene="rplP"
                     /locus_tag="PLK08_06285"
     CDS             complement(2160..2579)
                     /gene="rplP"
                     /locus_tag="PLK08_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPS53141.1"
                     /translation="MPLMPKRVKHRKQMRGRISGNATRGNRVNFGEYGLQTMEPAWIT
                     ARQIEAGRIAATHFLRRIGKVYIRIFPDKPISGKPLEVRMGKGKGETKHWVAVVKPGQ
                     VMFEIGGVSEEAAKDALRRVAMKLPVRCRMVARKHGV"
     gene            complement(2725..3525)
                     /gene="rpsC"
                     /locus_tag="PLK08_06290"
     CDS             complement(2725..3525)
                     /gene="rpsC"
                     /locus_tag="PLK08_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPS53142.1"
                     /translation="MGQKVNPIGFRTGVTLGWRSRWFAPKSNYGEFLVEDAKIREFLD
                     QRLNRTPPFAAVSSVEIERTREEVKVILKTARPGIVIGPKGAEVDKIKNALEELTDRR
                     VNVNIVEIRNPDLDARLIGEGISEQLKRRASFRRCMKQKAEAAMQAGARGVKIMLSGR
                     LGGAEMSRNEKMVLGSVPLHTLEADVDYGYTPCFTTYGVIGIKVWVYRGLFSEQLTEE
                     EMIAQQQARNAADGRRPRRRRRSRGKGIAQQQQTSAGEADAPTATSEE"
     gene            complement(3564..3893)
                     /gene="rplV"
                     /locus_tag="PLK08_06295"
     CDS             complement(3564..3893)
                     /gene="rplV"
                     /locus_tag="PLK08_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPS53143.1"
                     /translation="MAWNATHKFARMSARKMRLVADMVRGRDVQDALNILKFSPNRAS
                     GMISKVIKSAVASANEGEADVDTLIVKEIRIDEGPVMKRFRPKDRGRAYNILKRTSHI
                     SVVVDVD"
     gene            complement(3937..4245)
                     /gene="rpsS"
                     /locus_tag="PLK08_06300"
     CDS             complement(3937..4245)
                     /gene="rpsS"
                     /locus_tag="PLK08_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPS53144.1"
                     /translation="MTRSAKKGPYVNERLFAKVEKAKEAGDRTPIKTWSRDCTIVPEF
                     IGQHFSVHNGKVFTNVLITEDMVGHKLGEFSPTRSFRGHSGAKAAAAASSPAAAPAKA
                     "
     gene            complement(4290..5084)
                     /gene="rplB"
                     /locus_tag="PLK08_06305"
     CDS             complement(4290..5084)
                     /gene="rplB"
                     /locus_tag="PLK08_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPS53145.1"
                     /translation="MRSEVTSNSPEKSLLRPLKKTGGRNFKGVVTSRFRGGGHKRRYR
                     LIDWKRNKDGMAAEVMTIEYDPNRTCNIALVQYEDGEKRYILSPVGLKVGSKVYSGIG
                     SEPTVGNCLPLRKIPLGLDIHNIEMTAGRGGQLVRTAGGTAKLVAREGDRATIILPSG
                     EMRILHASCRATLGQLGNIEHMSVRWGKAGRKRYLGRRPHVRGSCQNPVDHPMGGGEG
                     RRSGGRHPTSPTGKLSKGGKTRNRRKTSNKMILRRRRNTRTGQLTL"
BASE COUNT          968 a         1503 c         1282 g         1331 t
ORIGIN      
        1 ccttcacata ttttagaggc gcggatatta accaccgaat gcttcgcctg tatggcgttg
       61 cggattcggg tcagcatgtc tgcgatggga tcagagagac tcataatcca ttccttccat
      121 taccaactgg cttttttcat acctggaatc agcccgtcca gggccatctt cctcaaacac
      181 aaacggcaga cgcgaaactt gcgatacacg cttctggacc gtccgcacaa ctggcagcgc
      241 cgtactgttc tgctggagaa cttcggcgtc agcttggctt ttgctatctt ggacttgctt
      301 gccatgactc ttgacccttt ttgtttagtc gcggaaaggc atgcccataa gtttcagcaa
      361 cttgcgagcc tgctcgtcat tttccgctga tgtctcgatt gagatattca tgccctgcgt
      421 gaacgtcatc gccgccggat cgatttccgg aaacaccgac tgttcactaa ggcccatgtt
      481 ataattgccc tggccgtcga acccggacgg attcaaaccg cgaaaatcct tcacccgcgg
      541 tatggccagc gagatgagac ggtcaagaaa ttcgtacatt ctctcacgac gaagcgtgac
      601 tttcatgccg gcgttcatgc cagcccgaac cttgaagttg ctgaccgact tgcgggcttt
      661 ggtaattatg ggtttctggc ccgaaagttt cgcaagctgc tcggcgcagg cttcatagcg
      721 tgccttgtcg gtagaatgct ggccaaggcc catcgatata accaccttct ttaccgccgg
      781 aatcgacatc ggattggcga tgccgtcggc tacgaaggct ggcatgattt cttttttgta
      841 tttttccttc agacgtgaca tttacgtttg cctcgcagat tacctggctt ttctgacaac
      901 acccaacgac ttgccgcttt tgacggcgag acgttccttg ctaccgtcgg cattgacggc
      961 gaagcgcacc cgcgtcggct gggaagtttc agggtctatc ggcatgacat tggaaatatg
     1021 cacgggcatt tccttcttca tgcgaccacc tttgggattc ttccggctgg gccgcagatg
     1081 acggaacacc tcgtttatgc ccttgaccag aaccctgctc tcatccggat atacacggat
     1141 aacttcgccg gtcttgccct tgtcatcgcc ggcgacgaca acgaccatgt cacctttttt
     1201 tactcgcagt gccatagtta caccacctcc gacgcaagcg acactatttt catataattt
     1261 ttttctcgca actcgcgggc taccgcaccg aagatgcgtg ttcccttcgg gttgccatcc
     1321 ttatcgataa gaaccgccgc gttcgaatcg aaccgcacat aactgccgtc ttttcggcgc
     1381 gtttttttgg cggctcgaac gatcacgcaa cgtaccacgt cgccggcttt gacgtcgccg
     1441 ccggggatgg ctttcttgac cgaggcgatt atgacatcac ccaggccagc cattctgcgg
     1501 gtcatgccgt gacggcgggt agttgaaccg cccagcaccc ggatacagcc tataatcctg
     1561 gcgccgctgt tgtcggctac gttgagtctt gtttctgcct gaatcacaat actatcctct
     1621 tatcattaac caaccgcggg ccgcactacg cgaaccagac ggtgtgcttt cttcttgctt
     1681 agcggccgaa ccggcacgat ttcaaccata tcgccgacct tagcctgctc cagctcgtcg
     1741 tgcaccgcca gcttcgtacg ccggcgaaga tatttgccat aagtctcatg cttgacgaga
     1801 ttgtctatct ggaccgaaac cgtcttgctg ccgctcttgg atatcaccgt gccaaccttg
     1861 gcgcgacggt tatgattttt tttctgtgct tcgttcgtca tttgaactcg cctttcctgc
     1921 tcttacgctg tctgctgtgc agaaagttca cgctggcgga taaccgtact gatgcgggcg
     1981 atatccttgc gggttttggt tatctgcgaa gtgttttcca gtttttcagt caccgattgg
     2041 ctgcggaggt cgaatatccg gcgtttgagg ttcgccagtt cagcctgcag ttccgcgtcc
     2101 gacatatttt ttatttcatt tatcttcatg gttggtcttc cagagatttt ttcatgccac
     2161 tatacgccgt gcttgcgggc aaccatacgg cagcgaaccg gcagtttcat cgccacgcga
     2221 cgcagtgcgt ccttggcagc ctcttccgaa acgccgccga tttcgaacat tacctggccg
     2281 ggcttgacca ccgcaaccca atgcttggtt tcgcccttgc ctttgcccat tcgaacttcc
     2341 aacggtttgc cggatatcgg cttgtcgggg aatattcgaa tgtaaacctt acctatacga
     2401 cgcaggaagt gagtcgcggc gatacggccg gcttctatct gtcgggccgt tatccacgcc
     2461 ggctccatcg tctgaagtcc gtattcaccg aagttcacgc gattgccgcg ggtcgcattg
     2521 ccgcttattc tgccgcgcat ctgcttgcgg tgtttaactc ttttgggcat caaaggcatc
     2581 ttatcttacc tcgttaccaa tccggcagac ttcggcaatt cgccgccatt ccgcctttgc
     2641 ctatttcctt gacacgctat cttatcgtca aatcttcaaa atatctcgcc gccatgcggt
     2701 tcgaaaatct gaaaatcacc aaaatcactc ttccgatgtc gccgttggcg catcagcctc
     2761 gccggctgac gtctgctgct gttgtgctat gcctttgccg cggctgcgac gacggcgacg
     2821 cggccgacgg ccgtcagctg cgttacgcgc ctgctgttgt gcaatcatct cttcttcggt
     2881 cagctgttcg ctgaaaaggc cgcgatacac ccacaccttt atgccgatga caccataagt
     2941 ggtgaagcac ggcgtatagc cgtaatcgac gtcggcttca agggtgtgaa gcggcacact
     3001 gccgagaacc atcttttcgt tgcgggacat ttccgcaccg ccaagacggc cggagagcat
     3061 tatctttacg ccgcgggcgc cagcctgcat ggcggcttcc gccttctgct tcatgcaacg
     3121 acgaaaactc gcacgccgct tgagctgttc gcttatgccc tcgcctatca gccgcgcatc
     3181 caggtcggga ttgcgtatct cgacaatatt aacattcacg cgacggtcgg tcagttcttc
     3241 cagggcattt ttaatcttgt cgacttcggc gcctttgggc ccgattacaa tgcccggccg
     3301 tgcggtcttg aggattacct tgacctcttc acgggtgcgc tctatctcga cggaacttac
     3361 agctgcgaac ggaggcgtcc ggttgagccg ctgatcgaga aattcgcgaa tcttcgcgtc
     3421 ttcgacgagg aattcgccgt agttgctctt gggtgcaaac cagcggctgc gccatccgag
     3481 cgtcacacct gttctgaatc cgattggatt tactttctgg cccatataaa atgcctcgcg
     3541 tctgtgcttc aatccttaaa aagttaatct acgtcgacaa ccacggaaat atggctggtg
     3601 cgtttcaata tgttatatgc cctgccgcgg tctttgggac gaaaccgctt catcacgggc
     3661 ccttcgtcga ttcgtatctc cttgacaatc agggtatcga cgtcggcttc gccttcattg
     3721 gcgctggcca cggcggattt gataactttc gatatcatgc ccgacgccct gtttggggag
     3781 aacttgagga tgttcaatgc gtcctgcaca tcccggcccc tgaccatatc ggcgaccagc
     3841 ctcattttgc gggccgacat gcgggcaaac ttgtgtgttg cgttccaagc catcttacga
     3901 tgctccattt tcaatctcga ttaaataacg ttcagcctat gccttggcgg gggctgcagc
     3961 tggtgacgat gcagccgcgg cagccttggc gccgctatgg ccacggaagc tgcgggtagg
     4021 actgaactcg ccgagtttat gccctaccat gtcttcggta atcagtacgt tcgtgaacac
     4081 tttgccgttg tgcaccgaaa aatgctggcc tatgaattcc ggcactattg tgcagtcacg
     4141 cgaccaggtc ttgatgggcg tacgatcgcc ggcttcttta gccttctcga ccttggcaaa
     4201 aagtctttcg ttcacatagg ggcctttttt cgcacttcgc gtcatggctt atcctcttgt
     4261 tggttcaatc attactatcc gattattcat tacaacgtca actgccctgt acgggtgttg
     4321 cggcggcgac gcagtatcat tttgttcgac gtcttgcgtc tgttgcgtgt cttgccgccc
     4381 ttggagagct tgcccgtcgg cgaggtagga tgacggccgc cggaacgtcg gccttcaccg
     4441 ccgcccatcg gatggtcaac agggttctgg caactgccgc gaacgtgtgg ccgacggccc
     4501 aggtaacgct tgcggccggc tttgccccaa cgaacggaca tatgttctat attgcccagc
     4561 tggccgagag tcgcgcggca tgacgcatga agtatgcgca tttcgcccga cggcagaata
     4621 atcgtcgcgc ggtcgccttc gcgggcgacg agcttggctg ttccgcctgc agtgcgtacc
     4681 agttggccgc ctctgccggc tgtcatctca atattgtgaa tatcaaggcc cagcggaatc
     4741 ttccgcagcg gcaggcagtt gccgacggtc ggctcgctac cgatgccgct atacaccttg
     4801 ctgcccacct tcaaacccac aggcgagaga atgtatcgtt tttcgccatc ctcgtactgc
     4861 accagcgcaa tattgcatgt acggttagga tcatattcaa tggtcataac ctcggcagcc
     4921 attccgtcct tgttccgctt ccagtctatc aggcggtagc gacgcttgtg cccgccccca
     4981 cgaaacctcg acgttacaac tcccttgaaa ttgcggccgc cggtcttctt gagcggacgc
     5041 aacagcgact tctccggcga attcgaggtg acttccgaac ggat
//
LOCUS       DAONFI010000164         5080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19425_length_5080_cov_3.697711, whole genome shotgun sequence.
ACCESSION   DAONFI010000164 DAONFI010000000
VERSION     DAONFI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19425_length_5080_cov_3.697711"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLK08_06310"
     CDS             <1..596
                     /locus_tag="PLK08_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53146.1"
                     /translation="QWLVFDEFDSGVHVCNVDYCADWPMYRAIDFGYSAPLVCLWIQV
                     TPAGNVHVIDEYVRTRLPIAKHAAEIRRRDCGPVQMTFVDPAGKQKESTSGAACTELL
                     AAEGIACSFRASTINEGLELIRAALTPAVGEPKLYINPRCRVLIESFNNYHYPSPESS
                     ADQDVPVKDGPDHAVDALRYFFVNRARPTIGISRTRY"
     gene            740..1609
                     /locus_tag="PLK08_06315"
     CDS             740..1609
                     /locus_tag="PLK08_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPS53147.1"
                     /translation="MSEKIFFNGELVEPSRAVVNINDPAVLHGSSVFEEMRVCNGVVW
                     RFDEHLRRLADNVDFLGLRTSATSGELVDGTYAAIDANDIRQGRCRIVLTPGEFGGKP
                     NVAVVASALPEYPQSWFDDGIDVVVSSLKIFRGDPLAGRDTGCNLWRLIALQEAARKK
                     AHEALLYNDINQLAQASMANVFLVIAGKVFTPPRDTPVTPGITRQAVMDICGRLNISY
                     DCDTPLTVKEMLDADEMFLTNVGVGVRPVVRIERHEVGNEKPGEITRQLMTELAALIE
                     NECKDRNIFQLDK"
     gene            1741..2844
                     /locus_tag="PLK08_06320"
     CDS             1741..2844
                     /locus_tag="PLK08_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPS53148.1"
                     /translation="MKVSDVVSAMENMAPSKYAMDWDNTGLLVGDGEAAVNRVLFCID
                     MTQKVLDEAIREKAQMVIAYHPVIFKGVKSVTAQSSPVLWAAMRSGMSVYCPHTALDV
                     AKGGVNDSLADAVGMDLSEAEPLEFHEFGEKMKLIVFVPASERDKVCRAAFAAGAGII
                     GEYEQCSFAVAGEGSFLPGENANPTVGSCCRHETVPELRVEMIVPREKLDCVCRAIRS
                     AHSYEEPAIDIYKLADEPGEFGMGRIGGLRKSTTLAAFVRRVKKRLGVKRVLLASGGD
                     LNRMVTTVAVGAGSCGDMWKKAAGRADIFVTGEMRHHDALAAVEAGLCVICVGHGNSE
                     RHVLGLLAERLASKVHGIKMIISQADRDPLEIV"
     gene            complement(2936..4012)
                     /locus_tag="PLK08_06325"
     CDS             complement(2936..4012)
                     /locus_tag="PLK08_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS53149.1"
                     /translation="MTDRLRFNNQMHYQPIDRCFNMEDGYWPDNYQQWNIFVENGVTT
                     EEQANIFFNFDRIENINGNTWLYPEFENKIIEERGDKLLIQNNDGVLAEVPRDGHDTI
                     PHYTQSPITTPNDWQRVKEEHMNRDHPGRKVDVAALMAKYPAERDYPVGVNCGSMIGN
                     IRNMLTFEGLAYACFDYPEMVEDMVEQCCLLVEDFLDQVLTIIDFDYASGWEDICFKN
                     GPIVSVDFFNSVVVPRYKRISKKLHAHGVDIWYTDCDGDVRPILDGLLEGGINCLFPF
                     EVMGCSHPGELLDKYEGRLRIMGGFNKMKLIDGPQAIKEYMETLVPYVKRGGYIPFCD
                     HRCPPDVSPENYLYYLDLKEKMFG"
     gene            4154..5029
                     /locus_tag="PLK08_06330"
     CDS             4154..5029
                     /locus_tag="PLK08_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPS53150.1"
                     /translation="MDRKNIYMDKSKQIKSVPIVKPDDVVPVVRHSNYLSPASGTVWG
                     PRVLLDIELILIVDGQFTYKDIRGVSETFASNDLCLILPGIEHTFSCAASQRKGKPFF
                     ACIHLDLLPNKSFLAGDYRLDPLPPLKCRVNNFREVNEFFKQMDAVYNSSDKYRDELV
                     FLTAKLIWLYLSRQWTASDSEQLSPTMQEMTAFLNERFMYSVGRRDLAKKFGVTPQYV
                     NRLFQRELKMTPTEYLNRYRVMQAAEYLRHGGMKVSDAAVLVGFDDPFYFSRIFKRYM
                     GLTALSFIKKHEKWC"
BASE COUNT         1317 a         1096 c         1325 g         1342 t
ORIGIN      
        1 ggcagtggtt ggtgtttgac gaattcgact ctggcgtaca cgtatgcaat gtcgattatt
       61 gcgcagactg gccgatgtat cgcgccatcg acttcggata ctccgcgccg ctggtgtgtc
      121 tgtggataca ggtcacgcca gccggcaatg tgcatgtgat agacgaatac gtccgtacca
      181 ggctgccaat cgcgaaacat gcagccgaaa tccggcggcg ggactgcggg ccggtacaga
      241 tgacgttcgt agacccggcc ggcaagcaga aggaatccac cagcggggca gcctgcacgg
      301 aactgctggc tgctgaggga atcgcctgct catttcgagc cagtacaata aacgaaggcc
      361 tggaactcat aagagccgcc ctcacgccgg ctgtcggcga gccgaaactg tatatcaatc
      421 cgcgatgtcg cgtacttatc gaatctttca acaactacca ttatccgtcg cctgaatcat
      481 cggctgacca ggatgtaccc gtaaaggacg ggccagacca tgcggtagat gcgctgcggt
      541 attttttcgt caatcgtgca cggccgacaa taggcatttc gcgaacgaga tattgatatc
      601 aaccgacatg acttccgaat agtcatgtcg gaaaaagtta aaaaaagtta aaaaacttct
      661 gataatattt ttgaggaata tgccgaaacg ataaaacttc ggcttgcatt attttcagta
      721 acaggtatag acttatggca tgagtgaaaa aatatttttt aacggcgaac tggtcgagcc
      781 gtcgcgggcg gtagtgaaca taaatgatcc tgcggtattg cacggcagca gcgtgttcga
      841 ggaaatgcgc gtgtgcaacg gcgtagtctg gcggtttgat gagcatttgc ggcggctggc
      901 agacaatgtg gattttttgg gtttgcggac gtcggcaacg agcggcgaac ttgtggatgg
      961 cacgtatgct gcgattgacg ccaatgacat ccggcagggc cgttgtcgca ttgtcctgac
     1021 gcctggagag tttggcggta aacctaatgt ggctgtggtg gcgtcggctc tgccggagta
     1081 tcctcaatcg tggtttgacg acggcataga tgtggtggtg tcgtcgttaa aaattttcag
     1141 gggcgacccg ctggctggaa gagacaccgg ctgtaatctg tggcgattga ttgcactgca
     1201 ggaggcggct cgcaaaaagg cacatgaggc gctgctttac aacgatatca atcagcttgc
     1261 ccaggcaagc atggctaatg tttttctggt aatcgcgggc aaagtcttta cgccgcctcg
     1321 cgatacgcct gtcacgccag gcataacgcg gcaggctgtc atggacatat gcggcagatt
     1381 gaatatatcg tatgattgcg atacgccgct tacggtcaag gaaatgctcg atgccgacga
     1441 gatgtttctc acgaatgtcg gcgtgggtgt aaggccggtc gttagaatcg aacgacacga
     1501 agtcggaaat gaaaaacctg gagaaattac gaggcagcta atgactgaac tggcggcact
     1561 gattgaaaat gaatgcaaag acagaaacat atttcagctt gacaaatgat tgccgttaaa
     1621 tcatatccat acataaagca agtatttacc tttacgccta gtactctgga aaccgttaaa
     1681 tcggtcagtt tgaataaaat aaattgatat tgctgccttg cggcggcggg aggtggtgca
     1741 gtgaaagtca gcgacgtggt ttctgcgatg gaaaacatgg caccgtcgaa atatgcgatg
     1801 gattgggaca acacgggtct gctcgtcgga gacggcgagg ctgccgtcaa ccgcgtcctg
     1861 ttttgcatag atatgactca aaaagttctt gacgaagcaa ttcgcgaaaa ggcacagatg
     1921 gtcatagcgt atcatccggt cattttcaaa ggcgtgaaaa gcgtgacggc gcagtcgtcg
     1981 cctgttctgt gggcagctat gcggtcaggc atgagcgtgt attgtccgca tacagcgctg
     2041 gatgttgcaa aaggcggtgt gaacgactcg ctggctgatg ctgttgggat ggatttgtct
     2101 gaagcggagc cgcttgagtt tcacgaattc ggcgaaaaaa tgaaactgat agtgtttgtt
     2161 ccggcatctg agagggataa agtctgcaga gcggcatttg cggctggtgc aggcataata
     2221 ggcgaatacg agcagtgctc atttgccgta gccggtgagg ggagttttct gccaggcgaa
     2281 aatgcaaacc cgacggttgg cagttgctgc cgccatgaaa ctgtacctga attgcgagtc
     2341 gaaatgatag ttccccgtga aaaattagac tgcgtctgtc gggctattcg ttcggcgcac
     2401 agttatgaag aacccgcgat tgatatttac aaattagccg atgaacctgg cgagtttggt
     2461 atggggcgaa taggcggatt gcgaaaatcg acaactctgg cggcgttcgt gcgaagagtc
     2521 aaaaaacgtc ttggcgtgaa aagagtgctg cttgccagcg gcggtgactt gaatcgcatg
     2581 gtgacgacgg ttgcagttgg agcaggctct tgtggcgata tgtggaaaaa ggctgcaggg
     2641 cgggctgata tatttgtaac cggcgagatg cgacatcacg atgcgctggc ggcggtggaa
     2701 gcgggattgt gtgtgatatg cgtcggccat ggcaattcag agcggcatgt attgggattg
     2761 ctggcggaac ggcttgcatc gaaagttcac ggcataaaaa tgataatatc acaggctgac
     2821 cgcgaccctt tggaaattgt ctaacacata tatcgcttga ctaaagcatg ctctagagtc
     2881 gaaagttaca gtgtgcacct ttatgaattt attataatag tgctctacat tatattcagc
     2941 cgaacatttt ttctttcagg tcaagataat aaaggtaatt ttcaggtgat acgtccggcg
     3001 ggcaacggtg gtcgcagaat ggaatatacc cgccacgttt tacataaggc acgagcgttt
     3061 ccatatattc cttaattgct tgcggaccat ctatgagctt cattttgttg aatccgccca
     3121 taatgcgcag tcgtccttca tatttatcca acagctctcc tggatgcgaa caacccatga
     3181 cttcaaatgg gaaaagacag ttaataccgc cttcaagcaa gccgtcgaga attggtcgaa
     3241 catcgccgtc gcaatcggtg taccatatgt ctactccatg cgcatggagt tttttactta
     3301 tgcgtttgta acgcggtacg acgacactat tgaaaaagtc cactgacact atcgggccat
     3361 ttttgaagca gatatcctcc cagccgctgg cgtaatcaaa atcgattatg gtgagcacct
     3421 ggtctaaaaa atcctctacc agcaggcaac actgttcgac catatcttca accatttccg
     3481 gataatcaaa acacgcatac gccagcccct cgaaagtgag catattacga atattgccta
     3541 tcatcgaccc gcagtttacc ccgactggat aatcgcgttc agctggatat ttcgccatca
     3601 acgccgctac gtcaactttg cggccaggat ggtcacgatt catatgttct tctttcacgc
     3661 gctgccagtc gtttggcgtg gtgattggcg actgcgtgta atgagggatt gtatcatggc
     3721 cgtctcgcgg tacttctgca agcacaccgt cattgttctg tatcaacagt ttgtcgccgc
     3781 gttcctctat gattttgttc tcaaactcag gatataacca tgtatttcca ttaatatttt
     3841 ctatgcgatc aaagttgaaa aaaatattgg cctgttcttc agtggtgacg ccgttttcta
     3901 caaatatatt ccactgttgg taattgtcgg gccagtatcc gtcttccata ttaaaacagc
     3961 ggtcaatcgg ttggtaatgc atctggttat tgaaacgcaa ccggtctgtc atatttcctt
     4021 cccatttcgg ccggcatggt ataatgtttt tttgtgtcat ttgtgtaacc ttattttaaa
     4081 gaatttttga aagtcagtta attatattca ttgatttaca taatgattat agctaaaaat
     4141 ggatattatg agaatggaca gaaaaaatat atacatggac aaaagtaaac aaataaaatc
     4201 tgtaccgata gtaaaacctg atgatgttgt gccggtggta cgacattcaa attatttatc
     4261 acctgcatca ggaactgtgt gggggccgag agtgcttctt gatatcgaac tgatattgat
     4321 agtcgatgga caatttacat ataaagatat acgcggagtc agtgaaactt ttgcctcgaa
     4381 tgatctttgt ctaatattgc caggtattga acatacattc agttgcgcag catctcaacg
     4441 aaaaggcaaa ccgttttttg cctgtattca ccttgattta ctgcctaaca agtcattcct
     4501 cgccggcgat tatcgtctcg accctcttcc gccgcttaag tgccgtgtga ataatttcag
     4561 ggaagtcaat gagtttttta aacagatgga tgcggtttat aattcctcgg ataaatatcg
     4621 tgatgagttg gtttttttga cggcgaaatt gatatggttg tatctgtcgc ggcagtggac
     4681 agcatccgat agtgaacaac tttcaccaac tatgcaagaa atgacggcat ttttgaatga
     4741 acgctttatg tactctgttg gccgcagaga tttggccaaa aaattcggcg ttacgccaca
     4801 atatgtcaat cgactttttc agcgcgaatt gaaaatgacg cctacggaat atcttaaccg
     4861 gtatagggtg atgcaggcgg ctgaatatct gcggcatggc ggaatgaaag tgagcgatgc
     4921 tgcggtactg gtgggtttcg atgacccgtt ttatttttcg cgtatattta aaaggtatat
     4981 ggggcttaca gccttatcat ttataaagaa gcatgagaaa tggtgctaga acctatccca
     5041 aaaaccttgg ctgcctgcaa tcggctcttt tgcagcctgg
//
LOCUS       DAONFI010000165         5064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19500_length_5064_cov_3.914953, whole genome shotgun sequence.
ACCESSION   DAONFI010000165 DAONFI010000000
VERSION     DAONFI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19500_length_5064_cov_3.914953"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1976
                     /locus_tag="PLK08_06335"
     CDS             333..1976
                     /locus_tag="PLK08_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HPS53151.1"
                     /translation="MKITAVAAVLLSAVVVWADGQAAVQSVRQPSILVIFSYAPAFKW
                     TKQEMQGIMDALEKEGFGNSRISMEYLNVTKDPSEENLENFAKLLIRRYQKSDIDLIM
                     CCDNDAIYFIVNQGRDFLPSVPVLFCGLNRLSPDLFNRNRVLTGIIESPDVQTNISLM
                     RKILPDTKKIFVISDYHSPSSIAVMEQVGPTLKEIGKTGIDIETAPDVSVDELVKKVS
                     ALDKHTQVLLLQYKTDNKGRPVDILNFVKRLSQNCPVPIIGLWDVYLDSGIVGGYMVD
                     GYRQGYEMGHMAGQVLAGMNPAVMPLIRNKGNCYEFDFLELQRFDIPLSVLPEGSNVI
                     NTCYRVRRSGDTLKKILGGAVVLCIIMLIPSALLFMRHRYVMHKIKNTDRIIQHIIDA
                     VPACMIATDNEKKILFANKACAEMTGMPVEKILGRNISEIISGKEGLAIVDDVLNSGL
                     PRYKELFHRLPDGRMTICQAFIRPALINNKQGLIIYGLDISQYKHTENQLKKIELQRE
                     QVLNSLSDGVILLDNALNVSMGQQKIGRNTWRPDSADDW"
     gene            1966..3714
                     /locus_tag="PLK08_06340"
     CDS             1966..3714
                     /locus_tag="PLK08_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS53152.1"
                     /translation="MIGKKCFTVKNHKIPCRECAALSTLRSKEPHSCVLEVNNKNILI
                     KAVPVFDEDVNIISVVETIQDISSQVKARKEAVNKMEMFQLLLDMLPAGILIINSDRQ
                     IVNANRYALDLLGVVTFDNIKGTLCHNRICPEADSDCPILNADKKVESRQCRILTIQG
                     QELPVLKTVRPVNYMGEEMVVEVFLDMRQHNMYVDNLLQTEKLQAVALFAKGLAGEFT
                     SKTADIFDKIAAMKKELSDNKCLKLLDSILNTTLRISEISDKLISFSRMDIAGSAEVD
                     LHEVIRHISVYMEHTLREEGIEMKINLSARHHVIVGDSLHLERSILNLCSNSQNAMPD
                     GGTLIIATTNENIDESSLLHAIRKLPVGRYVRVQVIDTGHGIAPEVQSHIFEPFFSTR
                     STASGVGLSAVFGTIKAHNGSIDVNSTPGLGTTFNIYLPVVSKSKTSADSPAEPAAVD
                     NSRYEILVVDDDQIARTIISEMLRRMGYGVSNAASGREAVDIYLSRRNAIKLILLDIT
                     MPDMSGREVFDIIREKDPDVKIMILSADLHEENVNYILNAGGMGYLAKPVTQKNLQIA
                     ISRALDGMMTLPADLN"
     gene            complement(3723..4817)
                     /gene="ispG"
                     /locus_tag="PLK08_06345"
                     /gene_synonym="gcpE"
     CDS             complement(3723..4817)
                     /gene="ispG"
                     /locus_tag="PLK08_06345"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPS53153.1"
                     /translation="MADMNLPEITRHKTRVAKVGSLTIGGDSPITVQSMTNTRTADAA
                     ATIAQVLRLAEAGAEIVRVATPTQEDTAALPEIVAASPVPIVADVHFHFQRAIEALQA
                     GVAKIRLNPGNISDRDKVKLVISEAASRGAAIRIGVNEGSISDRRDMDKYRQDREKPL
                     AELMAEKMAEYLAIFEQEGFDNLVLSAKSHDAYTTVAVNRELARRWNYPLHLGVTHAG
                     TPATAAIRSAAALGTLLCEGIGDTLRISYAGDPVNEVMAANELLYSLRLRKREGIELI
                     ACPTCGRLQMDLSPVIAKMEELLKSVKKNLTVAIMGCVVNGPGEAENADIAICAGRDK
                     AVLYRRGQRIKNVKTEEIIPTLLKEIDSFQ"
BASE COUNT         1235 a         1177 c         1318 g         1334 t
ORIGIN      
        1 ctgcgggccg cacttgccga tggttttgcc ctgcaccgtg tcgatgaaga aatccgaatt
       61 tgtgccgtct atgcggccag acacttttat gaccagataa ccatcgacta tgtcccatgt
      121 aatgtcattc ataactagtt cctgcctcaa ttcaccctgg ttcatttttc acatgaaatc
      181 agccatttca tgttatctga ttatcggccc tgttgcaagt atattgaaag aatagtttga
      241 aaattgcaaa aggttgaact atctggtatc attcaattaa cgttcgttac agggtttatt
      301 tatgggagga acggtttgta atgccacggg ctatgaagat tacagcggtt gccgcagttt
      361 tgctgtcggc ggttgttgtt tgggcagatg gccaggctgc cgttcagtct gtcaggcagc
      421 catccatatt ggtgattttt tcctatgcgc cggcgttcaa gtggaccaag caggaaatgc
      481 agggcattat ggatgcgctg gaaaaagaag gctttggcaa ttcgcgaata tccatggaat
      541 accttaatgt cacgaaagac ccgtctgagg aaaatctgga aaatttcgcc aaactgctca
      601 ttcgccgtta tcagaagtcg gatatcgacc tgataatgtg ttgcgacaat gatgccatat
      661 attttatagt gaatcagggc agggattttc tgccgtccgt tccggtactt ttttgcggcc
      721 tgaaccggtt aagccctgat ttgttcaatc gcaacagggt attgacgggt ataattgagt
      781 cgcctgatgt tcagaccaat attagcctga tgcggaagat actgcccgat acgaaaaaga
      841 tattcgtcat ttccgactat cacagcccaa gcagcatcgc cgtcatggaa caggtcggcc
      901 cgacgcttaa agagataggg aaaaccggca ttgatatcga gacagcccct gacgtaagcg
      961 tggacgaact tgtcaaaaaa gtatcagccc ttgataaaca tacgcaggtt ttgctgcttc
     1021 agtataaaac ggacaataaa ggcaggcctg ttgatattct gaactttgtc aaacggcttt
     1081 cgcagaactg tcctgtaccg attatagggc tgtgggatgt ttatctggac agcggaattg
     1141 tcggcggtta tatggtggac ggttaccggc agggttacga gatgggccac atggccggac
     1201 aggtgctggc cggcatgaat ccggctgtaa tgccgcttat cagaaacaaa ggcaactgct
     1261 acgaatttga tttcctggag ttgcagcggt ttgacattcc tctttcggtt ctgccggaag
     1321 ggagcaatgt aatcaatact tgctatcgcg tccggcggag cggcgatacg cttaaaaaaa
     1381 tactcggcgg agcggttgtg ttgtgtatta tcatgttgat accgtcggcg ttgctgttca
     1441 tgaggcatcg atatgttatg cacaaaatta aaaacacaga cagaatcata caacatatca
     1501 tagatgccgt gccggcttgc atgattgcca ccgataatga gaaaaaaatc ctttttgcga
     1561 acaaggcctg cgctgaaatg accggcatgc ctgttgaaaa gattttgggc agaaatattt
     1621 ccgagattat atctggcaaa gagggactgg cgattgtgga cgatgtgctg aatagcggct
     1681 tgcccaggta caaggagctt tttcatcgtt tacctgacgg aagaatgact atctgccagg
     1741 catttatccg gcctgctctt ataaacaaca agcagggcct tattatctat ggccttgata
     1801 tttcacaata caagcatacc gaaaatcagt taaagaaaat agaactgcaa agagagcagg
     1861 ttctgaattc gctgtccgat ggcgtgatat tgctcgataa tgcgctgaat gtaagtatgg
     1921 gccaacaaaa aatcggacga aatacttggc gccccgattc agcagatgat tggtaaaaaa
     1981 tgttttacgg tcaagaatca taagattcca tgccgggaat gcgccgcgct ctcgactctc
     2041 cgcagcaaag agccgcattc gtgcgtgctt gaggtaaata acaagaacat actcataaaa
     2101 gccgtgcccg tgtttgacga agacgtgaac ataatttcag tagttgaaac cattcaggac
     2161 atatcatcgc aggtgaaagc tcgcaaggaa gccgtgaaca agatggaaat gttccagttg
     2221 cttctcgaca tgctgccggc tggaattctg ataataaaca gcgacaggca aatcgtaaat
     2281 gccaaccgtt atgcgcttga ccttctcggt gtggtaacat ttgacaatat caaaggcacg
     2341 ctctgccata accggatatg tcctgaagcg gactcggatt gtccgatact caatgccgac
     2401 aaaaaggttg agagccggca gtgcaggatt cttaccattc agggccagga actgccggtg
     2461 ctgaagaccg ttcggccggt aaattatatg ggcgaggaaa tggtggttga ggtttttctc
     2521 gatatgcggc agcataacat gtatgtcgac aaccttttgc agacggaaaa acttcaggct
     2581 gtcgcgttgt ttgccaaggg ccttgccggc gaattcacca gcaagacggc tgatatattc
     2641 gataaaatcg ccgccatgaa aaaggaactg tcggataata aatgcctgaa attgctggat
     2701 tcgatactta atacgacttt gcggataagt gagatttccg acaagctgat atcgttttcc
     2761 cgcatggata tagccggttc tgccgaggtt gatttgcacg aagtcatccg tcacatttcg
     2821 gtatatatgg agcataccct tcgtgaagaa ggcatagaga tgaaaatcaa tctctcggcg
     2881 cggcatcatg taatcgtcgg cgattcactg catctggaac gttcgatact caatctctgt
     2941 tcgaattctc agaacgccat gcctgacggc ggcactttga ttatcgccac taccaacgaa
     3001 aacatagatg aaagcagtct gcttcatgcc attcgcaaat tgcctgtcgg ccgttatgtt
     3061 cgcgttcagg tcatagacac cggccacggt attgcccctg aagtgcagag tcacattttt
     3121 gagccgttct tttcgacccg ttcgacggcg agcggcgtag gcttgtcggc tgttttcggc
     3181 actataaaag ctcacaacgg ttcgatagat gtaaattcaa cgcccggcct cggcacgacg
     3241 ttcaatattt acctgccggt ggtttcaaaa tcaaaaacat ccgccgacag tcctgctgag
     3301 ccggccgcag tcgataacag ccgatatgaa atccttgttg tcgacgatga ccagatagcc
     3361 cgaaccatta tttctgaaat gttgcggcgc atgggttatg gcgtatcgaa tgccgccagc
     3421 ggcagggagg cggtggatat ttatctttca cggcggaacg caataaagct gattctgctc
     3481 gacattacca tgccggacat gtctggccgt gaagttttcg acatcatcag ggagaaagac
     3541 cctgatgtca aaataatgat actttccgcc gacttgcatg aggagaacgt gaattatatt
     3601 ctcaacgccg gcggcatggg ttacctggcc aagccggtta cgcaaaaaaa tctgcagata
     3661 gcgatttcgc gtgcgttgga cggcatgatg acactgccgg ctgacttgaa ttgaagcctc
     3721 ttttactgga agctgtcgat ttctttaagc agtgtcggga ttatttcttc cgtttttacg
     3781 tttttgattc gttggccgcg gcggtagagg acggctttgt ccctgccggc acagatggcg
     3841 atgtctgcgt tttccgcttc gccagggccg ttgaccacac agcccatgat ggcgaccgtc
     3901 agattttttt tgacgctttt cagcaattct tccatttttg caattaccgg cgacaaatcc
     3961 atctgcagcc gcccgcaggt cggacacgcg ataagctcta tgccctctct ttttctgagt
     4021 cgcagcgaat aaagcagttc gttggccgcc attacctcgt tgaccggatc gcccgcatag
     4081 ctgattcgca gcgtatcgcc gatgccttca cataacagag tgcctagtgc ggcggctgaa
     4141 cgtatggcag ccgtagccgg cgtgcctgca tgtgtgacgc ccaggtgcag aggataattc
     4201 caccgcctgg ccagttcgcg gtttacggcg acggtggtgt aggcgtcatg gcttttagcc
     4261 gacaatacga gattgtcgaa tccttcctgc tcgaatattg ccagatattc cgccattttt
     4321 tccgccatca gttccgccag cggtttttcc ctgtcctgtc gatatttatc catgtctcta
     4381 cggtcgctta ttgagccttc gtttacgccg atacgtatgg ctgctccgcg gctggcagct
     4441 tcgcttatga cgagttttac cttgtcgcgg tcggatatgt tgcccgggtt cagacggatt
     4501 ttggctacgc cggcttgcag ggcttcgata gcccgctgga agtggaagtg cacatccgcg
     4561 actatcggta ctggtgatgc ggcgacgatt tccggcaggg cggcggtgtc ttcctgggtg
     4621 ggcgtagcca cgcggactat ctccgcccca gcctctgcca gccgcaacac ctgtgcgatg
     4681 gtagccgctg cgtcggcggt tctggtattc gtcatggatt gaacggtgat tggcgaatcg
     4741 ccgcctatcg tcagagagcc gactttggcg acgcgggttt tatgtctggt tatttcgggc
     4801 agattcatgt cggccatttt actcacggcg ggcctgaaat gaatcgaaaa tttctgcggc
     4861 gaaggtataa tgttgaaaaa ttatagaaaa atggacgaaa ttttattttg tacggcatgg
     4921 ggcgagcgca taatgggcgg cttatgttag ttgatgctga aataatggtt ttccttaaat
     4981 cctgcgggtt gagtgcgggt tttgaccttt ccctgcttga gcggctgtat gggctgttga
     5041 cggctggcaa cgctaggacg aatc
//
LOCUS       DAONFI010000166         5056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19551_length_5056_cov_3.783843, whole genome shotgun sequence.
ACCESSION   DAONFI010000166 DAONFI010000000
VERSION     DAONFI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19551_length_5056_cov_3.783843"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="PLK08_06350"
     CDS             complement(<1..1542)
                     /locus_tag="PLK08_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPS53154.1"
                     /translation="MNKKELSERDICTKFITPAIEKAGWDIQSQVREEVYFTDGRIYV
                     RGNSTARGQRKFADYILYYRPNIPVAVIEAKDNNHSTGDGMQQALDYAGILDIPCVFS
                     SNGDGFIFHNRSGDIVSEVSLPLDYFPSPEQLWSFYKQFKGIETPDQEQVVSQDYYFD
                     GSGRKPRYYQQIAINRAIEAIAKGENRILLVMATGTGKTYTAYQIIYRLWSAGVKKRI
                     LFLADRNALIDQTQRGDFRHFKDKMTVVKNHKVDKSYEIYLALYQGLSGSNPEQDIFK
                     QFSKDFFDLIVVDECHRGSAREDSAWRRILEYYSSATHIGLTATPKETEDISNIEYFG
                     DPIYTYSLKQGINDGFLAPYRVVRVGLNIDLEGWRPPAGYLDKFGNPVEDRIYNRTDF
                     DRNLVVEERRQLVSQKVTEFLKGTDRFSKTIIFCTDIEHAEGMRREMVNANGDLSKAN
                     PKYIMRITGDNDEGKRELDNFTNPEETYPTVAVTSKLMTTGIDAQTCKVIVLDSNIQS
                     MTEFKQ"
     gene            complement(2274..4019)
                     /gene="recJ"
                     /locus_tag="PLK08_06355"
     CDS             complement(2274..4019)
                     /gene="recJ"
                     /locus_tag="PLK08_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPS53155.1"
                     /translation="MDCFEPRYRWEISEPWPQAAELAERLAVSPVIAQILHNRGIDDE
                     LSAKRFLSPKWSDLLDPHELGGCEQAAKVIAAAVRAGKRMVIYGDYDVDGITAITILH
                     NCLKHAGADVGYYVPNRLEEGYGVNSAALGELIDGGAELIITVDCGISASANFAEFAG
                     RADFVVTDHHSLSDAVPDISAVVHPAVNGYSNPSLCGAGVAMKLAWQIARELEGVDRV
                     SRHMRTMLINATALAALGTVADVVPLVGENRTIAVLGLLAVASTEHVGLRAMVNAFSS
                     GGPLSVRDIGFGLAPRINAAGRMGHASNAIEMLLTDSPQVAVKITRELDELNEQRKIV
                     EKDITSQAIEMVHAAGMDGDDCRAIVLSSEDWHNGVIGIVASRLVDRFNKPAVMIAFD
                     SNNRGQGSARSIDGYHLADALHACGEFLISSGGHAKAAGLRIEKSQIADFTRVFLAHA
                     AANISCKELKPAVKVDVESVLAELCHMNTAAVFEKMAPFGAGNNQPLVLVRGCKIACP
                     PKRMGKTGTTLNLMLRQGQRGPVMRCVGFGMGGYADVLQGVRDVDVLVTPVINRFNGN
                     LTVELQLKDISPAVN"
     gene            complement(4200..4502)
                     /locus_tag="PLK08_06360"
     CDS             complement(4200..4502)
                     /locus_tag="PLK08_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPS53156.1"
                     /translation="MNARELDVRDTDKGAILAVKAVPGASRDKIAGLLGGALKVTTST
                     AAEKGRANKSVAAIIAKSLGVAPGNVEIIAGMTNAHKEFIVVGLKADDVRNRLEKI"
     gene            complement(4509..>5056)
                     /locus_tag="PLK08_06365"
     CDS             complement(4509..>5056)
                     /locus_tag="PLK08_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPS53157.1"
                     /translation="GKVFVAGDNIDTDQIIPAKFLSYNPADNDERKFFGRYAMAGVPD
                     KESGLPQGNVKFTPEDEFKSQYVVIVAGKNFGCGSSREHAPLAIAEAGCHIIVAESYA
                     RIFFRNCVNGGYCAPMVTGVPIYREFVTGQDVQVDLDKGELKNLATGKTYKLEPLGDV
                     ADIVAVGGVFEYARKTGMLQS"
BASE COUNT         1301 a         1297 c         1096 g         1362 t
ORIGIN      
        1 ctgcttgaac tccgtcatag actggatgtt cgagtctaaa acaattacct tacatgtttg
       61 ggcatcaatt cctgtagtca ttaatttgga tgttacggca acagtgggat aagtttcttc
      121 tggatttgta aaattatcaa gctcacgttt accttcatca ttgtccccag taatgcgcat
      181 aatatatttg ggattggctt ttgaaagatc accgttggca ttgaccattt ccctacgcat
      241 tccttcggca tgttcaatat ctgtacagaa gataattgtt ttgctgaatc tatctgttcc
      301 cttaagaaac tcagtgactt tctgggaaac aagttgccgc cgttcttcaa ccaccaagtt
      361 acggtcaaag tccgtccggt tgtagatacg atcttcaact gggttcccaa atttatccag
      421 ataaccggca ggaggtcgcc aaccctccaa gtcaatattc aatccaacgc gaacaacacg
      481 atatggagcg agaaagccat cattgatgcc ttgtttcaaa gagtaagtat aaatcggatc
      541 gccaaagtat tcgatatttg atatatcttc tgtttctttt ggagttgcag tcaagccaat
      601 gtgtgtagca ctactgtaat attccaggat acgccgccat gcactatcct cacgagcaga
      661 acctctatgg cattcatcca ctacaataag atcgaaaaaa tccttagaaa attgcttaaa
      721 aatatcctgt tcaggattac tgccggataa accttgatag agagccaaat aaatctcgta
      781 tgatttgtca actttatggt ttttgacaac tgtcatcttg tctttaaaat ggcgaaaatc
      841 tcctcgctgg gtttgatcaa tcaaagcatt tcgatcggca aggaacagaa tgcgcttctt
      901 tactccagca gaccaaaggc gataaataat ttgataagca gtataggttt ttccagttcc
      961 tgttgccatt accaacaaaa ttcgattttc gcctttagca attgcttcaa tcgcgcggtt
     1021 tatagcaatt tgttgataat aacgaggctt cctgccgcta ccgtcgaaat aatagtcctg
     1081 tgagacaact tgctcttgat caggagtttc aatacctttg aactgcttat aaaagctcca
     1141 tagttgctca ggtgatggaa agtaatcgag gggaagcgat acctcgctta cgatatctcc
     1201 gctacggtta tgaaatataa aaccatctcc attactgctg aatacacaag gaatatcaag
     1261 aataccagca taatccaaag cttgctgcat tccatctcct gtggagtgat tattatcttt
     1321 tgcttcgata acagcaactg gaatatttgg gcgataatac aagatataat ccgcaaattt
     1381 tcgctgaccg cgagcagtag aattaccgcg tacatagata cggccatccg taaaatatac
     1441 ttcttctctc acttgcgact gaatatccca tcccgctttt tctatcgcag gggtgataaa
     1501 cttggtacaa atatcacgtt ctgaaagttc ttttttattc atagtcgccc atattgcctt
     1561 ttttcctctg ccgctacctc gtttcccata gcttgccctt taatacttga aattaatgat
     1621 gttatttaac cttttttgga gacggatttc aattttattg cttaaaaaac ggccctttgc
     1681 agtttatgaa ggtggatatt tgacactaaa aacttacaac catttacagt ttaatgagtt
     1741 atcaaaatat tagccttaaa ggaattgagt atgaatacca gaaggctgtc gtagtttggg
     1801 gtggcttcag tcagctacta ttatcacccg atctgcgaca acctggccgc aaggggataa
     1861 gctgaaaacc gccaagcctg ggggaatttc gattatctca gccattggac acccctttga
     1921 ttatttgacg cattttggta ataatctggt ggcgttttga gtcggtcaga accgaccaag
     1981 aatctattag gaattgaagt tcagacggca atgcgtcgga tttagaaaca tcattttcag
     2041 aaattgcgtt tttgttgggc tttttgtgag gtgttattgt ccttccggga gtttgggtct
     2101 tgttcgtatc cactcgcagc agcattcacc aactcgcaat gactcgcatt tcccccaatg
     2161 ttttcaggca taaaatcgac tttttgcatt tctatatgac cgcttgcggc tagatacggc
     2221 gtcggatttt gcaaccaaat tttccggatt caacgcctgc ctcaaaatta tcatcagttc
     2281 acggcaggcg aaatatcctt tagctgcaat tcaaccgtta aattgccgtt aaaacggttt
     2341 atgaccggcg taaccagcac atcgacatcc cgcacgccct gaagcacatc ggcataccct
     2401 cccatgccaa agccgacgca tcgcatcacc ggcccacgct ggccctgacg caacataaga
     2461 ttcaacgtcg taccggtttt gcccatgcgc tttggcggac acgcgatttt acagccccgc
     2521 acaagcacca gcggctgatt attgccagcg ccgaacggcg ccatcttttc aaaaacggcg
     2581 gcagtattca tgtgacaaag ttccgccaac acggattcga catcaacttt cacggctggt
     2641 ttcaactcct tgcaagaaat attcgccgcc gcgtgtgcca aaaatacccg cgtaaagtcg
     2701 gctatctgcg atttctctat tcgcaagcct gctgcctttg cgtggccgcc actggatatc
     2761 agaaattcac cgcacgcatg caatgcgtcg gcaagatgat agccgtctat ggagcgtgcc
     2821 gaaccctggc cgcgattatt ggagtcgaaa gctatcatta cagccggctt gttgaacctg
     2881 tcaaccagcc gcgacgccac aatgccgata acgccgttgt gccaatcttc ggacgaaagc
     2941 acaatcgcac gacagtcatc gccgtccatg ccggcggcat gcaccatctc aatagcctgc
     3001 gacgtaatgt ccttttctac aattttccgc tgctcgttga gctcgtcgag ttcccgggtg
     3061 atttttacgg caacctgcgg agaatcggtc aaaagcattt caatcgcatt tgaagcatgg
     3121 cccattcggc cggcagcatt tatgcgcggc gccagcccga aaccgatatc ccgtaccgat
     3181 aacggccctc cgctgctgaa cgcattgacc atcgcccgca agccgacatg ctccgtactc
     3241 gccacggcaa gcagtccgag aaccgcaatc gtgcgatttt ccccaacaag cggaacaacg
     3301 tccgcgacag tacccagagc agcgagggct gtggcgttta tcagcattgt acgcatatgg
     3361 cggctgacac ggtcgacgcc ttcaagttcg cgagctatct gccaggcaag tttcatggcg
     3421 acgccggctc cgcaaagcga tggattggaa tacccgttta cggctggatg gacgacggcg
     3481 gatatgtccg gtacggcgtc cgaaagtgag tgatggtcgg taacgacaaa atcggcacgg
     3541 ccggcgaatt cggcaaaatt cgctgacgca cttatgccgc agtcaaccgt gataataagc
     3601 tcagccccgc cgtcgataag ttcacccaac gcggcggaat ttacgccata cccttcctct
     3661 agacggttgg gcacgtagta accgacatct gcgccggcat gcttcagaca gttatgaaga
     3721 atcgttatcg cggtaattcc gtcaacgtcg taatcgccgt aaataaccat acgtttgccg
     3781 gctcgtaccg cggcggctat gacctttgcc gcctgttcgc agccgccgag ctcatgcggg
     3841 tcgagcaaat ccgaccattt gggcgaaaga aaccgctttg ccgacaactc atcgtcaatt
     3901 ccgcgattgt gaagtatttg ggcgatgaca ggcgacacgg caagcctctc ggccaattcc
     3961 gcagcctgcg gccagggttc agaaatctcc caacgatatc tcggttcaaa acaatccatt
     4021 gtcaaccgta atttttttaa aactttttaa acttttttaa aaaatttaaa aaatttaaaa
     4081 aattttagaa tttttcaggt ttttaatttt ttatttttat ttctattttt taaaaaaatt
     4141 caacgcttaa aacgcgaaaa tcaatcggcg agtaccacat attgcccgcg ttgtacgaat
     4201 cagattttct caaggcggtt gcggacatcg tcagccttca gcccaacaac gataaattcc
     4261 ttatgagcgt tggtcatgcc ggcaattatc tcaacatttc ctggagctac gccaagcgat
     4321 tttgcaatga tggcagcgac agacttgttg gctcgccctt tttcggcggc tgtcgacgtc
     4381 gtcactttca acgcaccgcc aagcagaccg gcgattttat ccctgcttgc gcccgggaca
     4441 gccttcaccg ccagaatcgc gcctttatcc gtatcacgta cgtcaagttc gcgtgcattc
     4501 atctgatttt acgactggag catgccggtt ttgcgggcat attcgaaaac accgccaacg
     4561 gctacgatat ctgcgacatc gcccagcggt tcgagtttat aggttttgcc ggtggcgagg
     4621 tttttcaact cacccttatc gaggtcgacc tggacatcct ggccggtgac aaattccctg
     4681 taaatcggca caccggtaac catcggcgca caatagccgc cgttcacgca gttgcgaaag
     4741 aaaatccgcg catagctttc agcgacgatt atatggcagc cggcttccgc gatggccaac
     4801 ggcgcatgct cgcgagacga accgcaacca aagttctttc cggcgacgat gacaacatac
     4861 tggcttttga attcatcttc cggcgtgaat ttgacattac cctgcggcag gccggattcc
     4921 ttatccggca cgccagccat cgcatagcgg ccgaaaaatt tccgctcatc attgtcggcg
     4981 ggattataac tcaaaaactt ggccggtatt atctggtcgg tatcgatatt atcgccggct
     5041 acaaaaactt tgcctt
//
LOCUS       DAONFI010000167         5056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19552_length_5056_cov_3.516097, whole genome shotgun sequence.
ACCESSION   DAONFI010000167 DAONFI010000000
VERSION     DAONFI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19552_length_5056_cov_3.516097"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLK08_06370"
     CDS             complement(<1..260)
                     /locus_tag="PLK08_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53158.1"
                     /translation="MKFFIKKNRGFTLIELLIGIIMTAMLMSAISIVIATGMKGYEIS
                     NNATEQTQLIRNTLERISREIRSADEVTWNGTTLVINPGSSQ"
     gene            complement(278..1636)
                     /locus_tag="PLK08_06375"
     CDS             complement(278..1636)
                     /locus_tag="PLK08_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53159.1"
                     /translation="MTSLSAKKRNRGVAMIMAIVLMTLLMAVMLVLFGSSRKDITKTI
                     NQQSQFNARLAAESGMAFMRRHIEDCELYSAPTVSETLTGLYNFLLSDIGSVTRTTNT
                     ITVSSRTLPTGYFTARIATDTTNEFKLTVTGTYGTTTRVITLGYNRDAGSRSPIFDYG
                     FSSQGALRMTGVSTITGKNDPSESKVFMASGITDEIIKLMGTATIGGDVFSVNPDSYI
                     TDAGTNCSVGGAYGSAISDHLYNGVDNIPLPTIDITPFLSGVSFTSVTSSTNTSGVTL
                     SNIQVKAGTNPNFSGCTINGVIYIEAPNKVTFSGQSYITGVIVTQQGTSDSSSVISFT
                     GGGYISSPENLPDTAAYSTVRSLPGTALLAPGFSVKFTGNMSAMSGTMAADNFSFSGS
                     GSMDVKGSIICYKDGEMVTITGSQTILIDKSGDQSTPPGFTGGTLTVLAPKASSLTDI
                     GS"
     gene            complement(1648..3003)
                     /locus_tag="PLK08_06380"
     CDS             complement(1648..3003)
                     /locus_tag="PLK08_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53160.1"
                     /translation="MRQKRIKNRGVALVMAIILMAMLMAVMLILTAASQKDIAKTVNQ
                     QQLFNSRLAAESGFAFMRHNIEECELSSSSSMETVLDGLYSYLSTTIGSVSRVGTAIK
                     VAARNMPTGSFEATIAKYDDDELVLSVTGAYGSTTRTLTLNYACDAGSRSPIFDYGFS
                     SRGTLKMSGSCVIIGKNDSSEANVYMANMDSDKIISLSGVCCIGGKVFATNPDAYVNA
                     AYSCSVDGLYRENIPNSVYTGVDEIELPTIDITQFTNGVSFTDITSSSTTSGIVNLSN
                     IRIKAGTNPHFSGDVDITGVIYIESPNKVTFSGDVNLTGVIVADKGTSDSSCAINISG
                     GVKIHSPSELPDEEKYSNIRNQIGTSILAEGFSVTFSGNNESVDGTMAADTFSFSGYG
                     YMNVKGGIICYKDGEVVNLSGNQTIIIDRSDGITTPPGFTGGSTTVLAPESSTYVDSG
                     S"
     gene            complement(3003..3437)
                     /locus_tag="PLK08_06385"
     CDS             complement(3003..3437)
                     /locus_tag="PLK08_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS53161.1"
                     /translation="MRTQNVNKKGFTLAEVLIASLIAGLCLAGAMTAISSNVQLTIQS
                     EEMSQAVFLAQEIREWSLNISFASISTMAMSSVSPKDSEGETLAGYNGWTETITVTYV
                     NPSTLADSASVTTMAKVGVTLQLNGKTVFATSWLVADFTGDT"
     gene            3704..3973
                     /locus_tag="PLK08_06390"
     CDS             3704..3973
                     /locus_tag="PLK08_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53162.1"
                     /translation="MFEKHTILGVHITDRLKDALEVQRILTQHGNQVKTRLGLHEIDE
                     AGKSGKNGLLILEMVGPDEGIRQMADKLNALDGIEVQSMVFKHTD"
BASE COUNT         1245 a         1105 c         1266 g         1440 t
ORIGIN      
        1 atctgtgatg aacctggatt tatcaccagc gtagtgccgt tccaggtaac ttcgtcggcg
       61 gaacgtattt cgcgtgaaat acgctccagc gtgttgcgga tgagctgcgt ctgttccgtt
      121 gcgttgttgc ttatttcata acccttcatg ccggtcgcta tgacgatgga aatagcagac
      181 attaacatcg cagtcataat tatgccgata agaagctcaa tgagcgtaaa gccgcggttt
      241 tttttaataa aaaatttcat ttactttcaa acaaaaatca cgaaccgatg tccgtcaaag
      301 acgacgcttt cggtgccagg acggtaagtg tgccgccggt aaagccgggc ggcgtggact
      361 ggtcaccgga tttatctatg agaattgtct gtgaaccggt tatggtgacc atttctccat
      421 ctttataaca tattatcgaa ccttttacat ccatagagcc ggagccggaa aaagaaaaat
      481 tgtccgccgc cattgtgccg ctcatcgcgc tcatattgcc ggtgaatttt actgaaaaac
      541 ctggggctaa taacgccgtg ccaggcaggc ttcgcacggt gctgtaggcg gctgtatccg
      601 gcagattttc cggactgctt atataaccgc cgcccgtaaa acttattacg ctgcttgaat
      661 cacttgttcc ctgttgggtc acgattacgc cggtaatgta gctctggccg ctgaatgtga
      721 ccttgttggg agcttctata tatattacgc catttatggt gcagccggaa aaattgggat
      781 ttgtgccggc ttttacctgt atgttgctaa gagttacccc cgaagtgttg gtagacgatg
      841 ttacagatgt aaacgaaacg ccgctaagga aaggcgttat gtcgatagtc ggcagaggaa
      901 tgttgtctac gccgttgtaa agatggtcgc ttatggccga gccgtatgcg ccgccgaccg
      961 aacagttagt gccggcgtcg gttatgtatg aatcaggatt aacggaaaaa acgtcgccgc
     1021 cgatggttgc cgtgcccata agtttgataa tttcatcggt tattccgcta gccatgaaca
     1081 cttttgattc cgacgggtcg ttcttgccgg ttatggtgct tacgccggtc atccgcagtg
     1141 cgccttgcga tgaaaaaccg taatcgaata tcggacttct gctgccggca tcgcgattat
     1201 agcccagcgt aatcactcgt gtagtagtgc cgtatgtacc ggtcactgta agtttgaact
     1261 cattggtcgt atcggtggct atcctggcgg taaaatatcc agtcggcagc gtacgggaag
     1321 acaccgttat ggtattggtg gttctggtta cgctgccgat gtcggaaagc agaaaattgt
     1381 aaaggccagt aagcgtctct gaaacggtag gagccgaata tagttcgcag tcctcaatat
     1441 gtcgccgcat gaacgccatg ccgctttccg ccgccagacg tgcattaaat tgcgactgct
     1501 gattgattgt ttttgtgata tctttccttg atgagccgaa aagcaccagc attacagcca
     1561 taagcaacgt cataagcact atggccataa tcattgccac gccgcgatta cgtttttttg
     1621 cggataagct agtcatgatt gcctctgtta cgagccggaa tccacatatg tcgaagattc
     1681 aggcgcaagt actgtagtcg aaccgccggt gaaacctggc ggagtcgtta tgccgtccga
     1741 cctgtctatg ataattgtct gattaccgga gaggtttacg acttcgccgt ctttatagca
     1801 tattatgccg cccttgacgt tcatgtaccc gtaacctgaa aacgaaaaag tatcggcagc
     1861 cattgtgccg tcgaccgatt cgttatttcc tgaaaaagtc acggaaaatc cctccgccaa
     1921 tatcgatgtg cctatctggt tgcggatgtt actgtatttt tcttcgtccg gcagttccga
     1981 tgggctgtgg attttgacgc cgccggagat gtttatggcg cagctgctgt cactcgtgcc
     2041 cttgtcggct actatgacgc ctgtgaggtt cacatcaccg gagaaggtca ccttgttcgg
     2101 cgattcaatg taaattacgc cggtgatgtc gacgtcgccg gagaagtgcg gatttgtgcc
     2161 ggctttaatc cttatgttgc tgagattcac tatgcccgac gtcgttgacg atgatgttat
     2221 gtcagtgaac gataccccgt ttgtgaactg ggtgatatcg atggtcggca gttcgatttc
     2281 atcaacgccg gtgtagactg agtttggaat gttttcccta taaaggccgt cgacggaaca
     2341 tgagtaggct gcattgacgt aggcatcagg attggttgca aaaaccttgc cgcctatgca
     2401 gcagacgcct gaaaggctta ttatcttgtc actgtccatg tttgccatat aaacgttggc
     2461 ttccgaggaa tcgtttttgc cgataattac gcaactgccg gacattttca atgtgccgcg
     2521 cgatgagaag ccatagtcga atatcggact tctgctgccg gcgtcgcagg cgtagtttaa
     2581 cgtaagcgtt cgggttgtgc tgccgtaagc tcctgttacg gagagtacga gttcatcatc
     2641 atcgtatttg gcgattgtcg cctcgaagct gccggtcggc atgttgcgag ccgccacttt
     2701 tatcgctgtg ccgactctgc ttaccgagcc tatggtggtt gaaagatacg aatacagccc
     2761 gtcgagcaca gtttccattg aactagacga tgacagctcg cattcctcta tgttatgtcg
     2821 catgaacgca aaaccgcttt ccgcggcaag cctggagttg aacagctgct gctggttgac
     2881 ggttttggcg atgtccttct gagaggctgc cgtcagaata agcatcacag ccatgagcat
     2941 cgccatgagt ataatcgcca ttaccaaggc gacgccgcga ttttttatcc tcttttgccg
     3001 cattacgtgt cccctgtgaa atcagccacc agccaactgg tggcgaaaac ggttttccca
     3061 ttgagttgaa gcgtaactcc caccttggcc atagtagtaa cactcgccga atctgcaagc
     3121 gtcgaagggt ttacataagt tacagttatg gtttccgtcc agccgttata gccagccaga
     3181 gtctcgcctt cgctatcttt tggcgaaacg cttgacatgg ccatcgtcga aatggatgcg
     3241 aatgaaatgt ttaacgacca ttcgcgtatc tcctgagcca ggaaaaccgc ctgcgacatc
     3301 tcttcgcttt gaatggtaag ctgcacgtta ctgctgatag ctgtcatggc gccggcaaga
     3361 cataaaccgg cgataaggga ggcaatgaga acctccgcca aagtgaaacc ctttttgttt
     3421 acgttttgcg tgcgcataga ttatcctacc gtttattata tcctctgtgg atgattaaaa
     3481 agtcgtactc tttgacaata aattactaga atatatgaaa aatacggaaa tccaaagcaa
     3541 tattgcaaaa taagggctaa cattatcaat ggaatttcag gcagccgccg ttaaaactgc
     3601 aaaaacaggc ggcagatttg atagattttt tattgaggac acttggtttt gtgcttacaa
     3661 taatgcgtgg ttaaaatatt caactttttt caggagactg taaatgttcg aaaaacacac
     3721 tattttaggg gttcacatca cggataggtt aaaagacgca ttggaggtac agcgtatact
     3781 tacgcaacac ggcaatcagg tcaagacgcg tttggggctg cacgagatag acgaagccgg
     3841 caaaagcggc aaaaacggct tgcttatcct tgaaatggtt ggccctgatg aaggcattcg
     3901 tcagatggct gataaactga atgctcttga cggcattgag gtgcagtcca tggtttttaa
     3961 acatacggat tgaagattgc gaaaatttag acttccatta tgacggctgc tataaaatac
     4021 ctattcatat gaaggggttt ttaatatgtc tttcgtaatt ttatggtttt tttgggaaaa
     4081 accgtaaatg gaagtatctg ccgccaggcg gacagacgac aacctgataa atatgatgct
     4141 gcgggttaaa atccgtagaa attcactgcg atatgggaag gcacgaggag aaaggacctt
     4201 tccgggttga gcccggcacg cttagccggg ctttttttat atgtatattc ggcgtggttc
     4261 tctaacatct tgtttttaag acaattatga taattgtggg cgttttgcct gacgtttatc
     4321 ttatgaatat ggaattgtgg gagatattcc aaaaataagg tgaaaaaact tgctttacag
     4381 ctgccctgaa tgttaagatt agcacggtaa gatgccggtc gtttaaggta cgattcggtg
     4441 catgttgtaa gtttttttac gatggcctgt tgttttgcga gtcaggtcgt cgggctagcg
     4501 gtgttccggc tgatttggcc gatgcagcat gttcgcccta tacgggtacg atattaattg
     4561 cagaattact tataaggaac agttgcggtg gaacaccgat taattcctgt tattgcagga
     4621 gcatagattt tattttgagt ccggatgtct tgcggcattc tgctgctgcc ctttgtgggc
     4681 gaaaaatctg gcagggacgc ctgaatgatg aaatttttaa aattccgtga ttttgaaaat
     4741 tttatgtaaa ggacgtgaat acccgccttg aaccgatacc gggtaaaagt ttgcgtatat
     4801 acgtataaaa actttccggt tataatttac gacatttgga ttttgtaact tcatgcaata
     4861 acggcagtaa taatcttttg cggctttttg cgcgtgtctt tggctgcatg gggttataca
     4921 attaataata aaaagtccga cttcctttcg tcgctttgat gggtcatcct tttgtacccc
     4981 gataagaggt gaaaggattt tttatggctg ccatatcagg tcagatggac atgggattgc
     5041 ttataggttg tcttgc
//
LOCUS       DAONFI010000168         5022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19725_length_5022_cov_4.548621, whole genome shotgun sequence.
ACCESSION   DAONFI010000168 DAONFI010000000
VERSION     DAONFI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19725_length_5022_cov_4.548621"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /gene="pheT"
                     /locus_tag="PLK08_06395"
     CDS             complement(<1..1709)
                     /gene="pheT"
                     /locus_tag="PLK08_06395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPS53163.1"
                     /translation="MNTSINWLKQYVDTDLSPEQLAELFTGIGLNCDGVEIQDSDAVL
                     DLEVTSNRPDCLGHIGIARELAAATGKELRLPKVSLPAGKGNINEITSVTVEAPELCP
                     RYTARVIKNVKIGPSPKWLTDYLTAVGIRCINNVVDITNYILMEYSQPLHSFDFDRLD
                     GKRIIVRRARKGEKIVSIDETTCELTDEMLVIADAKKPVAIAGIMGGLNSEVTDSTRN
                     ILLESARFDPLCIRRTSRALNLMSDSNYRYERGVDPVMLDEASRRACQLICELAGGEM
                     AEGVVDVWANPFREYTVKLRPQRTDKVLGIEIPSEKQAEILAGLGLGITKTSDGELLC
                     TIPSFRPDLVREIDLIEEIARLWGYDKIPLRHSVTHQVRAMGSHEKIRRLVEDILAAA
                     GYSEGIIFTFADDSETALFGYENPIHVDTRVRKSNNALRPTILPSLLRAFKSNQDVGN
                     TSLSLFEIASVFPPSSSQPDKPAECTELAMATTYDMQDVLGALQAAVARVAPQAELTV
                     TPQDFPGLESGECAAVLLDGEKIGMKVFLYFNEPLALPREHEFWKIHPELAGEPHVEP
                     ELN"
     gene            complement(2027..2100)
                     /locus_tag="PLK08_06400"
     tRNA            complement(2027..2100)
                     /locus_tag="PLK08_06400"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2064..2066),aa:Asp,seq:gtc)
     gene            2333..2668
                     /locus_tag="PLK08_06405"
     CDS             2333..2668
                     /locus_tag="PLK08_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53164.1"
                     /translation="MLQCETCEFFHRGPNGEVALSCDPFSNIKEPECLAKWQLVKINQ
                     MVGCYQATLRYYEKFAPMQEKMFNYMEKELNDINEADQWKTYDEEDGDDEHDLDSGEN
                     NFEDDEDWK"
     gene            2988..>5022
                     /locus_tag="PLK08_06410"
     CDS             2988..>5022
                     /locus_tag="PLK08_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPS53165.1"
                     /translation="MRDVIRVLFFSMLLTCATFAAPSMAVGETESKILKAAQLRNDAI
                     NESLAGNFNKGLTEIRESLTLNPDDPIAMEARKLLDTYDEQAKQIKKDRQSEYEYEVQ
                     RVMWALQVQKYFDSMAAAQKKLVDGLRKIINNPRVDDSDLVDFYHEVGIASSFEDASA
                     EEAAQMKKTSVEALDKSLKKIDEAISLVKDEKTPYARLFLENAQKVRSMLLRFREVWR
                     AADPSTPQSRWNAARDIFELEYDNRNDLNSLGIFLSYSPWKLALIYADSAKEMAPVDV
                     DVSKQEWFRLAVAEGRRQGDKAISEHLWRTALIAYSTLDDLLPADKDVTDTLEKVRTH
                     YRMGLIYGQKTKEETPDVSESLDPDEVGDADEEADEDITWKEMISRVDARMVRTAITR
                     LRKSYVKAIDFRELTRGALESVLILAETPQVVQTFPGLKDEKKRNAFIEALKKELEDI
                     NHPDRVDHVRLQMVLNRVLACNEETGVNIPAEVLVMEFTNGFLSKLDKFSSMIWPYEV
                     ENFTKSTMGHFTGIGVQIKKDKGEPLRVVTPLLDTPAHKAGIKAGDIILKVDGVDTKN
                     QSTDKLIKRIMGEPGTKVVLTIKRRGVAEPFDVPVVRQAVHIATIKGWQRYSDGKWDF
                     LLNREKGIGYIRITQFTEKTADALHEALAELHKQGISALVLDLRGNPGGLLQSA"
BASE COUNT         1234 a         1162 c         1405 g         1221 t
ORIGIN      
        1 cagttcaact ccggttccac atgaggttcg ccggcgagtt caggatgtat tttccagaat
       61 tcgtgttcgc ggggcagagc gaggggttca ttgaaataca gaaaaacctt catgccgatt
      121 ttttcgccgt cgagcagaac agctgcgcat tcgcccgatt ccaagcctgg aaaatcctgc
      181 ggcgttaccg tcaattcagc ctgcggcgcg acgcgagcga cggcagcctg caatgcgcca
      241 agtacgtcct gcatgtcata cgtcgtagcc atagccaatt ccgtacattc cgccggctta
      301 tccggctggc tggacgacgg cgggaaaacg ctggcgattt caaacaggct cagcgaggta
      361 ttgccgacgt cctgattgct cttgaatgcg cggagcaggg agggcagaat cgtcggccgc
      421 agggcgttgt tggatttccg cacgcgggta tctacgtgta tcgggttttc atagccgaac
      481 agggcggttt cagaatcgtc ggcgaatgtg aaaatgatgc cttcgctgta gccggctgcg
      541 gcgaggatgt cctcgacgag ccgacggatt ttctcgtggc tgcccatggc acgaacctga
      601 tgggtcacgc tgtgccgcag cggtatcttg tcgtaacccc acaggcgggc gatttcttcg
      661 atgaggtcta tctcacgcac gaggtccggc ctgaatgacg gtatggtgca gagcagttcg
      721 ccgtctgatg ttttggttat tccaagcccg aggccggcca atatttcagc ctgtttttcc
      781 gacggtattt cgatgcccag tactttgtct gtgcgctgcg gcctgagttt gaccgtatat
      841 tccctgaacg ggttagccca gacgtccact acgccttccg ccatttcgcc gccggcgagt
      901 tcacaaatca gctggcatgc gcggcgggac gcctcgtcga gcatgacggg gtctacgccg
      961 cgttcatagc gatagttgct gtcgctcatg aggttgagtg cgcggcttgt gcggcggatg
     1021 cagagcgggt cgaatcgggc ggattccagc agtatgttcc tggtcgagtc ggtcacttcc
     1081 gaattaaggc cgcccattat gccggcgatg gcgacgggct ttttagcgtc ggctatcacc
     1141 agcatttcgt cggtaagctc gcacgtggtt tcgtcgatgg agacgatttt ttcgcccttg
     1201 cgagcccgac gaacgatgat tcttttgccg tccagccggt caaagtcgaa actgtgaagc
     1261 ggctgtgaat attccatcag aatataattg gtgatgtcga ctacgttgtt tatgcagcgt
     1321 atgccgaccg ccgtcaggta atccgtcagc catttcgggc tggggccgat tttcacgttt
     1381 tttatcaccc gtgccgtata tcgcgggcag agttcgggtg cctcgacggt tacgctggtg
     1441 atttcgttta tgttgccttt gccggctggc agtgagactt tcggcagacg gagttccttg
     1501 ccggtggctg ccgccagctc gcgagcgatg ccgatgtgtc cgagacagtc gggacggtta
     1561 ctggtgactt ccaggtcgag tacggcgtcg gagtcctgta tttccacgcc gtcgcagtta
     1621 aggcctatgc ctgtaaaaag ttcggctaac tgctccggcg aaaggtctgt gtcaacatat
     1681 tgtttcagcc agttgatgga tgtgttcatt ttgtcaaaat ttctaaattt tctaaaaaaa
     1741 cgttaagagc gtctaacaaa atgatattgc attcggcttg ttggttgaat gtctggcggc
     1801 gtcaggctgc atcgcaagcc ctataaggct atctgcttcg ccttccttgc cagccaatca
     1861 accaactacg cctcggtgcg acatctcatt ttgttagaag ctctacataa catacgccaa
     1921 gaaaccaatg ataataagtt tagccggcat gtcaatcttg acgacggcga aatggcgggt
     1981 tggcaaataa aaaaggcttt gtacgataac gcacaaagcc tttcaacgag gccgacggga
     2041 atcgaacccg caaccactgg atcgacagtc cagtactcta accaattgag ctacggcctc
     2101 ttattcagat ttaacggcga tatccgccac tatccgccgc cgctttctta cgccggaatg
     2161 gggagtatat atactttgct ttttatgtca attcattttt taggattttt tgggttggaa
     2221 ctggcttttt tgcagacact tgtagggtta tggctgaaaa gcgaataatt ttttgcatcg
     2281 catggggtat ttagggtatg ataaatagat tatctggatt aggagtattt caatgctgca
     2341 atgtgaaacc tgcgagtttt ttcacagagg cccaaatggt gaagtggccc ttagttgtga
     2401 ccctttttca aatatcaaag agccggaatg cctggctaaa tggcagctgg tcaaaatcaa
     2461 tcagatggtc ggctgctatc aggcgacttt gcgatattat gaaaaatttg ccccgatgca
     2521 ggaaaaaatg ttcaattata tggaaaaaga gcttaacgat atcaatgaag ctgaccagtg
     2581 gaaaacatat gacgaagaag acggcgacga cgaacatgat ttggacagcg gcgaaaacaa
     2641 tttcgaggat gatgaagatt ggaaataacc catcgggcaa tatagggtgc aatgaatatc
     2701 catagggaat agcttgtcgt aagttttgta tcatgatgtg gttaggtggt ttgtggaaac
     2761 cgatgtcatg ttgaacgtaa agaagtatcc gaatcgccaa aggtaaagta gcgagcgtca
     2821 agagtcagct tatgagcagg ttccaacttt cagatatcgt ataacggtta tccaatacgg
     2881 aaaattgaaa agtgtttctc taaagtttgc ctcttgtagc ttgccacggt tcagaatggc
     2941 gattgtgtga taaaaaaaat ttatattata taaggatgta attggaaatg cgtgacgtaa
     3001 taagagtctt gtttttttcg atgctgttga cgtgtgcgac gtttgcggcg ccgtcgatgg
     3061 ctgtgggcga gaccgaatcg aaaatcctca aagccgcaca gctgcgaaac gacgccatca
     3121 acgaatcgct cgccggcaat ttcaacaagg gcctcaccga aatcagagaa tctctcacac
     3181 tgaatccgga tgaccccatc gctatggagg ccagaaaact tctcgatacg tacgacgagc
     3241 aggctaaaca gataaaaaaa gaccggcagt cggaatatga atatgaagtt cagcgggtga
     3301 tgtgggctct gcaggtccag aagtattttg attccatggc cgctgcgcag aagaaacttg
     3361 tcgacgggct acgcaagata atcaacaatc cgcgtgtcga tgacagcgac cttgtggatt
     3421 tttatcatga agtcggcatt gcatccagct tcgaggacgc gtctgccgaa gaggcggctc
     3481 agatgaagaa gacgtctgtg gaggcgttgg acaaatcctt aaaaaaaatc gacgaggcta
     3541 tttcgctcgt aaaagatgaa aagacgccgt atgcaaggtt gtttttggaa aatgcgcaga
     3601 aagtccgcag catgttgttg cgatttcgcg aagtctggcg tgctgcagac ccgtccactc
     3661 cgcaaagccg ctggaacgcg gctcgcgata ttttcgagct tgaatacgac aacaggaacg
     3721 acctgaacag tttggggata tttttatcat attcgccgtg gaagctggcg ttgatttacg
     3781 ctgactcggc taaggagatg gcgccagtcg atgtggatgt gtcaaagcag gagtggtttc
     3841 ggctggctgt ggcggaaggc cgtcgtcagg gggataaggc gatttccgaa catctctggc
     3901 ggacggcgtt gatagcttat tctacgctgg atgaccttct gccggctgat aaggatgtga
     3961 cggatacgct tgaaaaggta cgtacccatt accgcatggg cctgatttac ggccagaaga
     4021 caaaagagga gacgccagac gtttctgagt cgctggaccc cgacgaagta ggcgatgccg
     4081 acgaagaggc agacgaggat ataacgtgga aagagatgat atcccgtgtc gacgcccgca
     4141 tggttcgtac agcgataacg cgtctgcgta aaagttacgt caaggcgata gattttcgtg
     4201 aactgacccg aggcgcactt gagagcgtat tgattcttgc ggagacgccg caggttgtcc
     4261 agacgttccc cggcctgaaa gacgaaaaga aacgcaatgc attcatcgag gcgctcaaga
     4321 aggaactgga agacatcaat catcccgacc gcgtcgacca tgtacggttg cagatggtgt
     4381 tgaaccgcgt acttgcgtgc aacgaggaga cgggcgtcaa tattcctgcg gaagtgctgg
     4441 tgatggaatt taccaacggt tttttgagta agcttgacaa gttcagcagc atgatatggc
     4501 cgtacgaggt tgaaaatttc accaagtcca cgatgggcca tttcaccggc ataggggtcc
     4561 agataaagaa ggacaagggc gaaccgcttc gcgtagttac gccgctgctc gatacgcctg
     4621 ctcataaggc tggcatcaaa gcaggcgaca tcatccttaa agtggacggc gtcgatacga
     4681 aaaatcagtc caccgacaaa ctcatcaagc ggattatggg tgagccgggc actaaggtgg
     4741 tgctgacgat taagcgtcgc ggcgtagccg agccttttga tgtgcccgta gttcgtcagg
     4801 ctgtacatat agctacaatc aaaggctggc agcgttactc tgacgggaaa tgggattttc
     4861 tgctcaacag ggagaaggga atcggttaca tacgcataac gcagtttacc gaaaaaacgg
     4921 cggacgcgtt acacgaagcg ttagccgaac ttcataagca gggcatcagt gcgctcgtgc
     4981 tcgatttacg cggcaatcca ggagggttgc tccagagtgc gg
//
LOCUS       DAONFI010000169         5008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19808_length_5008_cov_4.599233, whole genome shotgun sequence.
ACCESSION   DAONFI010000169 DAONFI010000000
VERSION     DAONFI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19808_length_5008_cov_4.599233"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PLK08_06415"
     CDS             <1..1224
                     /locus_tag="PLK08_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53166.1"
                     /translation="HAFLAICFIYFALQAFCADAGQQPAGQLMLSVTGAMLILSRYEG
                     LFMVFVVCVLLMFLGRIAYAFFVGAVSLLPVSIYGFWCAGHGWYFLPNSVLLKGRTPG
                     RTFGDVIQFLAGGINAVEHNVHILILLAVSLAVLLLAYRRKICMSGRRAIVWTNWIFL
                     LTALLHMQFARTGWFFRYEAYLVFIGIVVTCASACVVFGNYVKSEIGVDALPRFAAVI
                     LLAVLAAFPFVKRGVLAFLTAPSAAENIYGQQYQMGLFLRQYYNGRAVALNDIGAACY
                     LADIRLLDLWGLGSMKPATLKMNRIYTSQEIYESARRADVEIAIVYDKWFAADNIGGL
                     PWQWIKIGQWQIPDNVIAGDDTVSFYCPEASEVERAVRNLRQFSGTLPADVKQLGRFA
                     GQCPDVGTSNKVPPH"
     gene            complement(1439..2692)
                     /locus_tag="PLK08_06420"
     CDS             complement(1439..2692)
                     /locus_tag="PLK08_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HPS53167.1"
                     /translation="MLKLARYSMGIGDRFAHQGKAQLAAMSKAQKTFGVEITPVWNKS
                     HREHVTIHTQPADVRREADAAVVALGWKGQYFCDADHISLKNVDLFLDSCDFFTLDVA
                     DFTGRPAADADIDAFAGKYAKKYARTLTLPGIGEIRISESMIRNIARKYLFAVQEAGR
                     IYRHILAARRTDNFITEISMDETDSPQTPIEMLFILAAVADEKIPAQTIAPKFTGRFN
                     KGVDYVGDIKQFEKEFREDLAVIAFAVKEFHLPANLKLSVHSGSDKFSIYEPICRAIK
                     QTGAGLHLKTAGTTWLEEIIGLASAGGEGLEIAREIYATALGRFDELAKPYATVIDIH
                     PQKLPSLELVNSWTPEQFADALRHVQDCPQYNPDFRQLMHVGYKVAAEMGGRYLAALE
                     KFEDVIAKNVTENIFDRHIKRIFID"
     gene            3170..>5008
                     /locus_tag="PLK08_06425"
     CDS             3170..>5008
                     /locus_tag="PLK08_06425"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPS53168.1"
                     /translation="MEMPKNSDKSHSHDLWQFRSTVEGIYIAIILAFVLRAFMIEAFV
                     IPTGSMANALYGEHYRLQCPACGYEYAYGYFPGDNDTGQHGVSVPLGKARAPRNAVCP
                     NCGYHYAFDELKADKVHGSGGDRVVVMKYLYEFAGPEPWDVVVFKNPQNNRENYIKRL
                     VGLPGETLEIVLGDVFVKQKGSSGFHIRRKPEYAQSDMWLVVYDNDFLPAGSLAEFIS
                     QKPQWVSKEKSGGVKTENQRVFHFDGIGNHGIVFRPGDKNAELGPLRPTNGYNPPQAE
                     QMTVSPQLDVCTDWKLSAVILGDGANSGRVQFDFELRGRKYRATFDLAGKVELAMGLR
                     SRKDDDGGWLVDKTVQQEFLADKGRQVELRHIDMMTSLWVDGKEILAVDDTLAVNTAD
                     GRTVAPCGYDTAKQLAAMPELLRQTSADIARLQTAGKPAQLHAAREKFDSLKQRWDWY
                     QNPSISITGSGKFSLAHVKILRDVYYTSPRIFTPRDNNANYDYVRKMLEQGGRGPNSW
                     KRDEDGNFIGWGVTGNPITLREFPDDSGRNEYYCLGDNSSQSFDCRSWIAAAPTLKLM
                     DKKGDFGYQLGTVPRYNIIGRAFFVFWPAGFTLPVLEMPVVPNAGRM"
BASE COUNT         1110 a         1126 c         1401 g         1371 t
ORIGIN      
        1 catgcatttc tggcgatttg cttcatatat tttgcgttgc aggcgttttg cgcagacgcc
       61 ggccaacagc cagccggcca actgatgttg tctgttaccg gcgcaatgct gatattgagc
      121 agatacgaag gccttttcat ggtattcgta gtttgcgtgc tgctgatgtt tttgggacgg
      181 atagcttatg cgtttttcgt aggggcggtt tctctgttac ctgtgagtat atacggtttt
      241 tggtgtgccg ggcacggctg gtattttttg ccgaattctg ttttgctcaa gggcaggacg
      301 ccagggcgga catttggcga tgtaatccaa tttttggccg gcggaattaa cgccgtcgag
      361 cataacgttc acatcctgat tttactggcg gtgtcgctgg cggttctgct gttagcctat
      421 cgcaggaaaa tttgcatgtc tggccgccgc gcgattgtct ggacaaattg gatttttctg
      481 ctcacggcgc tcctgcatat gcagtttgcg cgtacgggct ggtttttcag atacgaggca
      541 tatctggtgt ttatcggcat cgtcgtgaca tgcgcgtcgg cgtgcgtcgt atttgggaat
      601 tacgtaaaat ctgaaattgg cgttgatgcg ctgccgcgct ttgcagccgt gattttgctg
      661 gctgtccttg cggcgtttcc gtttgtcaag cgtggcgttc ttgcgttttt gacggcgcct
      721 agcgcggcgg aaaatattta cggtcagcag taccagatgg gtcttttttt gcgacaatat
      781 tataatggca gagctgttgc cctgaatgat ataggtgcgg cgtgctatct ggcggatatc
      841 aggctgctgg atttatgggg gctgggcagc atgaaacctg cgacgcttaa aatgaacaga
      901 atctacacgt cgcaggaaat atacgaatca gcccgacgtg ccgatgtcga aatagccata
      961 gtttacgaca aatggtttgc ggctgacaat atcggcggct tgccgtggca atggattaaa
     1021 attgggcagt ggcagattcc agacaacgtc attgccggcg acgatacggt ttctttttat
     1081 tgtcctgaag cgtcagaggt ggagcgggct gttcgcaatt taaggcagtt ttctggcaca
     1141 ttgcctgccg acgttaaaca gctaggcaga ttcgccggcc aatgtcctga tgttggtact
     1201 tccaacaaag tgccgccgca ttagagagac attccgaaat tgttgaaata ccattccgtt
     1261 tcgttaaaaa gtttttagct agagcattga aagattgaga gttcatattt ttgcgtcagt
     1321 gggtttgttc gtggcaagga aggaaaatga ggcatacttg gcagtattct gaagtttttc
     1381 tgacgcagcc acggacaaag caaaccagca aaaaaggaac tctcaagatt gaactgctct
     1441 agtctatgaa tattcgtttg atgtgacggt cgaaaatgtt ttccgtgacg tttttggcga
     1501 taacgtcctc gaatttttcc agcgcggcaa gatatcttcc gcccatttca gccgctacct
     1561 tgtagcccac atgcatcaac tgtcggaaat cgggattata ttgcggacaa tcctgcacgt
     1621 gtcgcagcgc gtcggcaaac tgttccggcg tccatgagtt gacaagttcg agggatggaa
     1681 gtttttgcgg atgtatgtcg attaccgttg catagggttt ggcaagttcg tcgaatcgac
     1741 ccagtgcggt tgcgtagatt tcgcgtgcga tttcaaggcc ttcgccgccg gcggaggcta
     1801 ggccgattat ctcttccagc catgttgtgc ctgcggtttt gagatgaagg cctgcgccgg
     1861 tttgctttat tgcacggcag atgggttcgt agattgaaaa tttgtcgctg ccggagtgga
     1921 cggaaagttt gaggttagcc ggcagatgaa attccttgac ggcgaaggcg attacggcga
     1981 ggtcttcgcg gaattccttt tcgaactgtt ttatgtcgcc gacgtaatcc acacccttgt
     2041 tgaatcggcc ggtgaatttc ggggcgattg tctgagccgg aattttctcg tcagccacgg
     2101 ctgccagaat aaagagcatc tcaatcggcg tttgagggga gtcggtttca tccatgctga
     2161 tttcagttat gaaattgtcg gtgcgtctgg cggcaagtat gtgacgatag attctgccgg
     2221 cttcctgtac tgcgaaaaga tattttctgg cgatgttacg tatcatgctt tcgctgatgc
     2281 ggatttcacc gatgcctggc aaggttagtg tgcgggcgta tttttttgcg tattttccgg
     2341 caaatgcgtc tatgtctgcg tcggctgcgg ggcggccggt gaagtcggct acgtcaagcg
     2401 tgaagaaatc gcatgaatcc agaaagaggt ctacgttttt gaggcttata tggtcggcgt
     2461 cgcagaagta ttggcctttc cagccgagtg cgacgacggc ggcgtcagcc tccctgcgta
     2521 cgtcagccgg ctgagtatgg atggtaacat gctcgcggtg cgacttgttc cacacgggcg
     2581 taatctcgac gccgaaagtt ttttgagcct ttgacatcgc tgcgagttga gctttgccct
     2641 gatgggcgaa cctgtcgcct atacccattg aatatctggc taattttaac attgttggcg
     2701 attcctggat ttttttaatt ttttaatttt tttaatatgt ccttcgtagt tctacggttt
     2761 ttaaaccgta gatgaaagta tccgccgtga ggctgacagg cgaaagcctg atgaatatga
     2821 tgctacgggt tttaacccgt agagattcac tttttttaat atgtccttcg taattctaca
     2881 tttttagagc caatatataa aagtcttcgt catcaggccg acaagccata ttttaattgt
     2941 atgtttggtt gacggttatt tcaaagcggg ctgattgaag attcgtctta aaacattacc
     3001 attggccggt gcaggctgat gggttggcgt tgaacgatac acgtgtttgt aatgccggcg
     3061 actgtctgcc ggctggtcaa attgagacgt aaataaaagt aaaaaatttt attactgtcg
     3121 gctttgagtt tttttgcttg gctgtagggg gtgaaatcgc tatcttttta tggaaatgcc
     3181 taaaaattcc gacaaatcgc acagccatga cctgtggcag ttcagaagca ctgtcgaggg
     3241 tatttatata gccataattc tggcgtttgt gcttcgcgcg ttcatgattg aggcgttcgt
     3301 aataccgact ggttcgatgg caaatgcctt atacggcgaa cattacaggc ttcagtgtcc
     3361 ggcttgcggt tatgaatatg cttacggtta ttttcctggc gataacgata ccggccagca
     3421 tggcgtttcc gttccgctcg gcaaagcccg tgctccgcgt aatgcggtat gcccgaattg
     3481 cgggtatcat tatgccttcg acgagctgaa agccgacaaa gtccacggca gcggcggcga
     3541 cagggtggtc gtaatgaagt atctctacga attcgccggc cctgagccct gggatgtagt
     3601 ggtcttcaaa aatccgcaga ataatcgaga gaactacata aagcgccttg tcggcctgcc
     3661 tggcgagacg cttgaaatcg tcctcggcga cgtgttcgtg aaacagaagg gcagttccgg
     3721 atttcatatt cgccgcaaac cagaatatgc gcagtcggac atgtggctgg tagtgtacga
     3781 taatgatttc ctgcctgccg gctcgttggc ggagttcatt tcccaaaaac cgcagtgggt
     3841 gtcgaaggaa aaatccggcg gcgtaaaaac ggaaaatcag cgtgttttcc attttgacgg
     3901 aatcgggaat cacgggattg tcttcagacc gggcgacaaa aatgctgaac ttggcccgct
     3961 gcggccgacg aacggctaca atcccccgca ggctgaacag atgactgttt ctcctcagct
     4021 tgacgtctgt acggactgga aactctcggc ggttattttg ggtgacgggg cgaattccgg
     4081 cagggttcag ttcgattttg aactgcgggg cagaaaatat cgtgcgacgt tcgatttggc
     4141 cggaaaagtt gaattggcga tgggccttcg cagccgtaaa gatgatgacg gcggatggct
     4201 ggttgataag acggtccagc aggagttttt agccgacaaa ggccgacagg tagaactgcg
     4261 tcacattgat atgatgactt cactgtgggt tgacgggaaa gagattcttg ccgtcgatga
     4321 tacgttagcc gttaatacgg cggacggtcg gactgttgcg ccgtgcggct atgataccgc
     4381 caagcagttg gccgccatgc cggaactgct gcggcagacg tcggcggaca tcgccagatt
     4441 gcagactgcc ggcaagcccg cacagttaca cgccgcaagg gaaaaattcg acagcctcaa
     4501 acaacgctgg gactggtatc agaatccgtc gatatccata acaggcagcg gaaaattctc
     4561 attggctcac gttaaaattc tgcgagacgt gtactatacc tcgcctcgca tttttacgcc
     4621 tcgcgataac aacgccaact acgattatgt ccgcaaaatg ctcgaacagg gcggcagggg
     4681 gcctaacagc tggaaacgcg atgaagacgg caatttcatc ggctggggcg tgacggggaa
     4741 tccgataacg ctgcgggagt ttccagacga ctcgggccgt aacgaatatt actgcctcgg
     4801 cgataattca tctcagagtt tcgactgccg ctcgtggata gctgccgcgc cgaccctgaa
     4861 acttatggat aaaaaaggcg actttggcta tcaattaggt actgttccac gttacaatat
     4921 catcggccgg gcgtttttcg tattctggcc ggctgggttt accctgccgg ttctggaaat
     4981 gcccgtcgtc cccaatgccg gcagaatg
//
LOCUS       DAONFI010000170         4979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_19950_length_4979_cov_3.979691, whole genome shotgun sequence.
ACCESSION   DAONFI010000170 DAONFI010000000
VERSION     DAONFI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19950_length_4979_cov_3.979691"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLK08_06430"
     CDS             <1..860
                     /locus_tag="PLK08_06430"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53169.1"
                     /translation="FDSPEESVKPQKSPQTGNTEASLRDALADFFKNAPAPQPRLNLP
                     AGEPRQHEYRHSQPIEHRPPQDFNGQPHTTKAIPSSEPDEKDYDSSAAPVTHRPLLQI
                     HNTYIVSQTPDGLSIVDQHALHERINYNELKRRLAEGNLTRQQMLIPQTIDVTPAEAA
                     LLEDASELLTKLGFEIEPFGPGTYAIQQYPTLLAQRGVRMTDFLRELIDTLRDDETAN
                     AERMLEKILAMMACKASIKAGDPLSQDEMQKLLERAEGIDKANACPHGRPTTLNISLK
                     ELEKSFKRI"
     gene            complement(921..2288)
                     /locus_tag="PLK08_06435"
     CDS             complement(921..2288)
                     /locus_tag="PLK08_06435"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPS53170.1"
                     /translation="MRDAVLERLASGRGRFLDELITFLKFQSIAECGNRKDCVPCAEW
                     LADLLERIGFDGGRILAGDCQPCVYAEKTVSADVPTILFYGHYDVQPADPLDEWETPP
                     FDPQIIDDYIYARGASDNKGQIMTWLRGLETLIAAGELSMNVKVLLEGGEEIGSPGMA
                     EVVLKNRQLLRADAVVASDMAWYADGVPSMTNMLRGLCAYEIIFRGPASDVHSGMYGG
                     LVVNPINELAALIASFHDASGRIAIPGFYDDVQDVPQEQLKKWEKLSFDEPACAKVVG
                     LNSFAGGEAGLEPIVRMWARPTLDCNGIVGGHTGSGSKTIIPAKASVKISTRLVADQE
                     PDRIESLLREFIFARVPAGIKCEFVTLAKSKAVIFPTDTPLVRRAAAAMEKVFGKAPV
                     FVGSGGSVPITSVLSELAGGNVFFMNLGLPDDRIHSPNERFRLDFFDRGAEMVCRFLG
                     SDTFK"
     gene            complement(2300..3160)
                     /locus_tag="PLK08_06440"
     CDS             complement(2300..3160)
                     /locus_tag="PLK08_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPS53171.1"
                     /translation="MKKYVMYMLMFPAFLAISGCPVPPTPPTPEDPWYWKDPVTKTEC
                     YLYIPEKYHHNKPMKVIVSCHGTPPFDVAEHHIRTWKYYGERYGCIIICPTMQGTDGV
                     LGDGPVLAMLENERNILSILSTLSYQYNIDRNNIMITGFSGGGFPAYWVGLRHPEIFS
                     VIVPQNCNFNRNNVDGWYPADATTVPFLIYYGEYDPAPIIVQSENAIKYLREKGFSVK
                     TEIIPGAGHDRHPEIAMEFFRKHWRTPAGTANQITPNGNRNTPTRMSPSTGSRTDDTD
                     RLGGPRPPAY"
     gene            3664..3810
                     /locus_tag="PLK08_06445"
     CDS             3664..3810
                     /locus_tag="PLK08_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53172.1"
                     /translation="MAKKVAKKAAAKKVAKKAAKKVAKKAVKKAAKKAAPKKAAKKKV
                     AKKK"
     gene            3947..>4979
                     /locus_tag="PLK08_06450"
     CDS             3947..>4979
                     /locus_tag="PLK08_06450"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPS53173.1"
                     /translation="MAAAKDAAAKAANAVAEGKLVGFATETVYGIAALANNPDTMARL
                     RELKNRPSNPFSVHIGSPSKAFDFISSENFPIRGRWLMQKTWPGPVTLLLPTGGRFAD
                     KKLSSLHATLTKNDILGLRCPSEPVAMMMLEMTPGVVVAPSANLAGNPSPKNADDVLR
                     DLDGRIDILLDTGPTQYGKDSTIVLFSGEDYEICREGVFDRRMISSVAGRKILFVCTG
                     NTCRSPMAAGIARAVLAEKFNCQPQELENKGIEICSAGIHAWEGGPATEQAVNVAEYH
                     GGDISSHKSRKISEDMLKSTDVVFCMTAGHLELAQTLARGMNCRVMLLDDCDVRDPIG
                     GGEDGENTTT"
BASE COUNT         1349 a         1371 c         1182 g         1077 t
ORIGIN      
        1 tcttcgacag ccctgaagag tccgtcaaac cgcaaaaatc cccgcaaacc ggaaacaccg
       61 aggcctccct gcgcgacgca ttagccgatt ttttcaaaaa cgctccagcc ccccaaccac
      121 gattgaattt accggctggc gaaccgcggc aacatgagta ccgtcattca cagccaatcg
      181 aacatcggcc gccgcaggat ttcaacggac agccgcatac gactaaggct ataccgtcct
      241 ccgaacccga tgaaaaagat tatgactcat ccgccgcacc tgtgacccat cggccactgc
      301 tgcagattca caatacctat atagtatcgc agacgcccga cgggctgagc atcgtagacc
      361 agcatgccct gcatgaacgg ataaattaca atgaactaaa acgacggctg gcagagggca
      421 acctcacgag gcagcaaatg ctgattcccc agactataga cgtcacgccg gctgaagcgg
      481 ccctgctgga agacgccagc gaactgctga caaaactggg attcgaaatc gaacctttcg
      541 ggcctggcac ttacgccatt cagcaatatc cgacgctatt ggcacagcgc ggcgtacgaa
      601 tgacggattt tttaagggaa ttaatcgaca ccctgcgcga tgatgaaact gcgaacgccg
      661 agcgtatgct ggaaaaaatc ctggccatga tggcctgcaa agcatccata aaagccggcg
      721 acccgctcag ccaagacgaa atgcaaaaac tgcttgaacg cgcggaaggc atcgacaaag
      781 ccaacgcctg tccccacggc cggcctacca cgctgaacat atccctcaaa gaacttgaaa
      841 aatcatttaa acgtatctga tatcactaca gcatgacaac catgctcgca agatacgaaa
      901 ctgccaaaat ttataaaaaa tcatttaaac gtatctgagc cgagaaaacg gcagaccatc
      961 tctgcgccac ggtcaaaaaa gtccagtctg aaccgttcat tgggtgaatg aatcctgtcg
     1021 tcaggcaggc cgagattcat gaagaaaaca tttccgccgg ccagctccga caacacggaa
     1081 gttatcggca cagacccgcc tgagccgacg aacaccggcg ctttcccgaa aactttttcc
     1141 atagccgccg ccgcacgccg taccagcggc gtatccgtcg gaaaaatcac agccttcgat
     1201 ttggccaacg tcacaaattc acactttatg ccagccggca cacgggcaaa aatgaattca
     1261 cgcaaaagag actctattct gtcgggctcc tggtcagcca ccagccgcgt ggaaattttt
     1321 accgacgcct tcgcaggtat aatcgtcttt gacccactgc cggtatggcc gccgacaatg
     1381 ccgttgcagt ccagcgtcgg ccttgcccac atccgcacaa tcggctcaag gccggcctcg
     1441 ccgccggcga aggaatttaa gcctacaacc ttcgcgcaag ccggctcatc aaacgaaagt
     1501 ttttcccatt ttttcagctg ttcctgcgga acatcctgca catcgtcata aaatcctgga
     1561 atcgcaatcc tgcctgaagc gtcatgaaac gacgcaatca gtgccgcgag ttcgtttatc
     1621 gggttcacca ccaagccgcc gtacataccg ctatgcacat cactagccgg ccccctgaaa
     1681 atgatttcat acgcacaaag gcccctgagc atattcgtca tggacggcac accgtcggca
     1741 taccaggcca tatcactggc gacaacggca tctgcgcgga gcagttggcg atttttcaga
     1801 accacttcag ccatgcccgg cgagccaatt tcctctccgc cttccagcag aaccttgaca
     1861 ttcatggaca gttcgccggc tgcaataagc gtctcaagtc cgcgcagcca tgtcattatc
     1921 tggcctttgt tgtcggaagc gccgcgggca taaatgtaat cgtcgattat ttgcgggtcg
     1981 aacggagggg tttcccattc gtcgagcggg tcagccggct gtacgtcata atggccgtaa
     2041 aacagaatcg tcggcacatc cgccgatacc gtcttttcgg cataaacgca aggctggcag
     2101 tctccggcaa gaattcgtcc cccgtcaaag ccgattcgct caagcaaatc ggccaaccat
     2161 tcggcacatg gaacgcaatc tttcctgttt ccgcactctg cgatggactg aaacttcaga
     2221 aacgttataa gttcatccag aaatcttccg cgtcctgaag ccagccgttc aagcacagca
     2281 tctctcatga tatctactct caatacgccg gcggccgcgg gccgcccaac ctgtccgtat
     2341 catcagttct gctaccggtt gacggcgaca tgcgggtcgg agtatttctg ttgccattcg
     2401 gcgttatctg gttggcagtg ccagccggcg tccgccagtg ttttcggaaa aattccatcg
     2461 ctatttcagg atgacgatca tggccggcgc caggaattat ttcggtcttt acggaaaagc
     2521 ccttttcgcg gagatatttg atggcgtttt ctgactgaac tataatcggc gcagggtcat
     2581 attcgccata atagatgagg aacggtacag tcgtggcatc ggcaggatac cagccgtcga
     2641 cgttgttacg gttaaaattg cagttctgcg gcactatgac gctgaaaatc tccggatgac
     2701 gcagtccgac ccagtatgcc ggaaatccgc cgccggaaaa acccgttatc attatattgt
     2761 tacggtctat gttgtactga tacgacagcg tagaaagtat gctaagtatg ttccgctcgt
     2821 tttccagcat cgccagtaca ggcccgtcac ccaatacgcc gtccgtaccc tgcatggtcg
     2881 ggcatatgat tatgcatccg tatctttcgc cataatattt ccaggtacga atgtgatgct
     2941 cggcgacgtc gaacggcggc gtgccatggc aactcactat aactttcatg ggcttattgt
     3001 gatgatactt ttccggaatg taaagatagc attccgtctt cgtgacaggg tctttccaat
     3061 accacggatc ttctggcgtc ggaggagtag gcggcacggg acaaccggat atcgcaagga
     3121 atgccggaaa catcaacatg tacatgacat attttttcat aaattttcct tcactgcgga
     3181 atcaaaaaaa tctgtcacca tcatataaat tccagccctc aagtggaaga tgaatttatc
     3241 attccgtcac aaactttaat gtccagggcc gtttcaagta aaaatttttc ggcgttttaa
     3301 aaaaattttt caggccgtca acagccgtca aacgccggcc agaacgccgt ttccggcctt
     3361 ggcggcgaaa aataacatat tcgtttcagt ttatatataa agaaccgaca gcatttaatc
     3421 cgcataaaca ttgaccaaaa tagccgtaca tgaagattgc atgaaaaaga cgcaaatttt
     3481 tcttaaactt tcacaacaaa aatagccgaa attatactat ggcgatacaa taacagccgc
     3541 gattcattat cgggttttca taaaacttaa cacttgtaag tcgttaaaaa tagaattata
     3601 acagggcaag taggttgtct tgttaaatgg atttttacaa ccgttattta aggagtccga
     3661 agaatggcta agaaagtagc aaagaaagct gcggccaaga aggtcgcaaa gaaggccgcg
     3721 aaaaaagtcg cgaagaaagc cgtgaaaaag gccgcgaaga aggccgcccc gaaaaaagct
     3781 gcaaaaaaga aagtcgccaa gaagaaataa gttttcgaag gcgtcttacc tgacggcgga
     3841 tatcggcaaa tgtcgatatc cgccgttttt tatttcacag tcaaaccgcc gtttcactat
     3901 aatatttttc atgaacaccc aggtaataaa agcatcggcc gatgacatgg ccgcagcaaa
     3961 agacgcagcg gcaaaggcag ccaacgccgt cgccgaaggc aaactggtcg gctttgccac
     4021 cgaaacggtc tacggcatcg ccgccctcgc caacaatcca gacaccatgg ctcgtctgcg
     4081 agaacttaaa aacaggccgt caaatccgtt cagcgtacac atcggctcgc cgtcaaaggc
     4141 atttgacttc atatcttcag aaaatttccc gatacgcggc cgctggctga tgcagaaaac
     4201 atggcccggc cctgttacgc ttctgctgcc cacgggtggc cgttttgccg ataaaaaact
     4261 ttcgtcgctg cacgccacgc ttacgaaaaa tgatattctt ggcctccgat gcccctccga
     4321 gcctgtggcc atgatgatgc ttgaaatgac gcccggcgta gtcgtcgccc caagtgcaaa
     4381 cctggcgggc aatcccagcc cgaaaaacgc cgacgacgtt cttcgcgacc ttgacggcag
     4441 aatagatata ctgctcgaca ccggcccaac gcagtacggc aaagacagta caatagtgct
     4501 gttcagcggc gaagattatg aaatctgtcg cgagggcgtc tttgaccggc ggatgatttc
     4561 atccgtagcc ggcaggaaaa tattgttcgt gtgtacgggc aatacctgtc gcagcccgat
     4621 ggcagccggc attgcacgtg cagttctggc tgaaaaattc aactgtcagc cgcaagaact
     4681 tgaaaacaaa ggcattgaaa tatgttccgc cggcattcac gcatgggaag gcgggccagc
     4741 caccgagcag gcggtcaacg tggcggaata tcacggcgga gatatttcct cgcataaaag
     4801 tagaaaaatt tcggaggaca tgttaaaatc aacagatgtg gtgttctgca tgactgccgg
     4861 ccatcttgag ctggctcaga cgctcgcacg cggcatgaat tgcagggtga tgcttttgga
     4921 cgattgcgac gtgcgggatc ccatcggcgg cggcgaagat ggtgaaaaca cgactacgc
//
LOCUS       DAONFI010000171         4922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_20306_length_4922_cov_3.775015, whole genome shotgun sequence.
ACCESSION   DAONFI010000171 DAONFI010000000
VERSION     DAONFI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20306_length_4922_cov_3.775015"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..1607
                     /locus_tag="PLK08_06455"
     CDS             492..1607
                     /locus_tag="PLK08_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53174.1"
                     /translation="MILLSSAVLPAWAVSVPTTNMALLSNGTVEVQYGTTVTGDIAGT
                     AGVSVHNNVLVTGSAYSGGASWVDSNSIITGSLNSNGNVSTGQGVSFGGDVTSGGTTW
                     LDNKTQVGGNVESSGSIDTGTNVKVTGDVNSGANIWLNNKTSVGGNASPGTNGGTLST
                     GSNVTIGGNTTPSNPTVPTVSIPTVTPGSVPSYSGSGSTWLPNNSSASITPGTYGSYS
                     TGTGVDLFFSAGEYNLGQIWLNNNSTLNFDTSAGDIVLNLFGDFNTGTGVTFIVTGDG
                     LVYVNVYDGNFWMAQNSTLDAIVRVYNGEFGMDTGATITGNVWASGDIYVGKNSTITY
                     RGDSPVPGVPEPLTVLSVFFATGGLGGYLAKRLKKSF"
     gene            1759..3489
                     /gene="recN"
                     /locus_tag="PLK08_06460"
     CDS             1759..3489
                     /gene="recN"
                     /locus_tag="PLK08_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPS53175.1"
                     /translation="MLRELHISNLAVIEDAAFDLQPGLNVFTGQTGAGKSLIIGAFEL
                     LLGLRGGGRDSAGMLRSGCDEACVCGLFEFSDSRIVHEIESIIGRQIEDSQMLITRRL
                     FATGRSSVSIDGQPATAAMLREIGQFLVDIHGQHDQQFLLKPSNQLEILDDFAAMCET
                     QKIFSQTFTRLRDLKKRRDDLNQSELIRRNQTELLEFQAGEIDAADIQPGEFESVREK
                     FERLSNIGTLKQDASQVAMAVYESEGSAVEILERAAKVMDELVSLDEKRLNEISQNFA
                     DATEMLRDVAAELNRYADNLEIEPGELEETQQRLDELNGMIHKYGPGREPTANDPLEI
                     VLKFREQVEMQLEKLRTENTELAGLDDEMSSLTKQLAEIGRKLTAGRNAAADKIAPLI
                     EKELSHLGMEQAKFRVQIVTHDVETCVSPAGLDEIEFMVQTNPGLAFQPLRAIASGGE
                     ISRIMLAIKSILAAGDRVSVLVFDEIDANIGGRLGAVVGRKMRDLARRGGHQVLCITH
                     LPQLAAFGDYHIHIEKHVEKGMTRTTVAPLEGSRRIDELAAMLAGHENVSQASITQAA
                     ELMQAAAKIS"
     gene            3723..>4922
                     /locus_tag="PLK08_06465"
     CDS             3723..>4922
                     /locus_tag="PLK08_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPS53176.1"
                     /translation="MDIELWIVAGATVLSLFFGLAAFTLSSFSWSKLGDTLAGTKQGK
                     KRLDTLEKHLKPLQMTLAMCKTIANFVLIAAMVCLMSDKTDFPTWQQAAIAIVICSVI
                     IAIFGVAIPFAWANYSGEKMLVATLPVLLGLRFILWPLVALMGLFDLPIRRLSGVHDD
                     EEEPTGEAKAEILQAAADGAAEGAVDAEEVEMIESVMEFAETRAAEIMTPRTDIFALS
                     ADTPWEEAVKTIHMAGHTRVPIYEDNLDNIVGIIYAKDLLKFMGHQPPSDLKAICRKP
                     YFVPDSKCLDNLLKDFKARKVHIAVVLDEYGGTAGLVTIEDVLEEIVGEISDEYDKTK
                     DAQVRRISQDSIDVDGRLRVDELNDELDIELPEDENYDTVAGFAAAYLGYIPPAGEKF
                     VAFGAEFT"
BASE COUNT         1312 a         1278 c         1251 g         1081 t
ORIGIN      
        1 gattaactca agatgctact ggtagccagt ttaaaaatca atttatggaa tacatccatt
       61 tttatggaga atttgatgat gtgcccatcg aattaatgaa ccaagaaatg gaaaaacact
      121 acccacattt gtttaaaaac agattggaat caaaagaatt ttctttttaa ttgccgtcga
      181 aaggtttcag taatgacgtc gaagcagaca tcctcatttt cattgtaaac cttatttctg
      241 ctgaggccgg ctttttgcag cctccctgtg aaagcacttt tggtggaaca atgaacgtta
      301 gttatcggac tcaaaatcaa taactcttta cggtcaaaac agtagaatcc caaaggctat
      361 taaaaattgt tattttatcc gaaacctttt tccttgattt acgtatataa tatagtagag
      421 aataaaagcg atttctgtac tgattgcaga aaggtttata tatgcagact attttcagga
      481 cattaataat tgtgatactg ttgtcgtccg ctgttttgcc ggcgtgggct gtcagtgtgc
      541 ctacgaccaa catggccctt ctttcaaatg gtacggttga agtacaatac ggcacaaccg
      601 tcaccggcga catagccggc acagccggcg tcagcgttca caacaacgta ctcgtaaccg
      661 gctcagccta ttccggcggg gcatcatggg tcgacagcaa ttcgataata accggttcgc
      721 tcaattccaa cggtaatgta agtaccggcc agggagtcag cttcggcggc gatgtgacca
      781 gcggcggcac aacgtggctg gacaataaaa cgcaggtcgg cggaaatgtg gaatcgagcg
      841 gctcgataga taccggtaca aacgtcaagg taacaggcga tgtgaattcc ggcgcgaata
      901 tatggctgaa caataaaacg agcgtcggcg gcaatgcgag ccctggtact aacggcggca
      961 cattgagtac cggcagcaac gtgacgatag gcggcaatac tacgccgtcc aatccaaccg
     1021 ttcctacggt gagcataccc actgttacgc ccggcagtgt tccgtcctat tccggcagcg
     1081 gcagcacatg gctgccgaac aattccagcg cgtccatcac gcctggcact tacggaagct
     1141 acagcacagg cacgggcgta gacctgtttt tctccgccgg cgaatacaat ctcggccaga
     1201 tatggctgaa taataacagc acgctgaatt tcgatacgtc ggccggcgac atagtgctga
     1261 atctatttgg agattttaac accggcacag gtgtcacatt catagtgacc ggcgacggcc
     1321 ttgtgtatgt gaatgtttac gacggcaatt tctggatggc tcagaactcg acccttgacg
     1381 cgatagtcag ggtttataac ggtgaattcg gcatggatac cggtgcaacc ataaccggca
     1441 acgtatgggc cagcggcgac atatacgtcg gcaagaattc taccataacg tatcgcggcg
     1501 attcgccggt tcctggcgta cctgagccgc ttacggtact gtcggtgttc tttgccacgg
     1561 gcgggttggg cggttatctg gccaaaagat tgaaaaagtc gttctgatta tttccaatat
     1621 ccaaaacgac tgattcacgc ccgtggaaaa atgacagatt tgagttcaat aaaaaaaacc
     1681 aaaagctgac gggtttgcaa caggcccgtc agctttttta tttgcttatc agccggcgtt
     1741 tgatacctta atgcgcaaat gctccgagaa ttacatatat cgaatctggc ggtaatagaa
     1801 gacgccgcct tcgaccttca gcccggcctc aatgtcttca ccggccagac cggcgcgggc
     1861 aaaagtctca tcatcggtgc gttcgagctt ctgctgggcc tacgaggcgg cggcagagac
     1921 agtgcgggca tgctgcgcag cggctgcgac gaagcctgcg tctgcggcct ttttgaattc
     1981 agcgacagtc gcatagtaca tgaaatagaa tccatcatcg gccgccagat agaagactcg
     2041 caaatgctca tcacgcgacg gctgtttgcc accggccgtt caagcgtatc catcgacggc
     2101 cagccggcta ccgcagccat gttgcgggag ataggccagt tcctcgttga cattcacggc
     2161 caacacgacc agcaattttt actcaaaccg tcaaaccagc ttgaaatact cgacgatttc
     2221 gctgcgatgt gcgaaacgca aaaaatattt tcgcagacgt tcacgcgcct gcgcgatttg
     2281 aaaaaacgac gcgacgatct gaatcaatcc gaacttatcc ggcgcaacca gacggaactg
     2341 cttgagtttc aggccggcga aatagacgcc gccgacattc agccaggcga atttgaatca
     2401 gtacgcgaaa aattcgagcg gctatccaat atcggcacac tcaagcagga cgcatcgcag
     2461 gtggcgatgg ccgtgtacga gtccgaaggc tcagccgtcg aaatcctcga acgcgcagcc
     2521 aaagtcatgg acgaactcgt ctcgctagat gaaaaacggc tgaatgaaat atcgcagaat
     2581 ttcgccgacg ccacggaaat gctgcgtgat gttgcggccg agctgaaccg ttatgccgac
     2641 aatctcgaaa ttgagcctgg cgaactggag gaaactcaac agcgtcttga cgagcttaac
     2701 ggcatgattc acaaatacgg ccctggccgt gagccgacag ccaacgaccc actggaaata
     2761 gtccttaaat tccgcgaaca ggttgaaatg cagctcgaaa aactccgcac tgaaaatacc
     2821 gagctggccg gcctggatga tgaaatgtca tcgctcacga aacagcttgc ggaaatcggc
     2881 cggaagctca ccgccggccg aaacgcggca gccgataaaa tcgcgcctct catcgaaaag
     2941 gaactctcgc atctcggcat ggaacaggcg aagttcagag ttcagattgt aacgcacgac
     3001 gtcgagacat gcgtcagccc tgccggcctg gatgaaatag agttcatggt acagaccaat
     3061 cccggccttg cgtttcagcc gctgcgggca atagccagcg gcggtgaaat ttcgcgaata
     3121 atgctggcca taaaatccat cctcgccgcc ggcgaccgcg tgagcgtcct cgtattcgat
     3181 gaaatcgacg ccaatatcgg cggccgtctc ggtgcggtcg ttggcagaaa aatgcgcgac
     3241 ctcgcccgcc gcggcggcca ccaggttttg tgcattacgc acctgccgca attagcagcc
     3301 ttcggcgatt atcacattca tatcgaaaaa cacgtagaaa aaggcatgac gcgaacaacc
     3361 gtcgctccgc tggaaggcag ccggcgcata gatgaactgg cagccatgct cgcaggccat
     3421 gaaaacgtct cgcaggccag catcactcag gctgccgaac tgatgcaggc ggcggcgaaa
     3481 atttcttgaa ccgccccgcc ctgttgcctt atactatcct gtgatatttt cagaaaattt
     3541 ccggttcaag gttttttaaa atcattttat agagtggttt taaaaaattt tgagaaccga
     3601 tctgcataat aaaaataatc ggacatcgga ttttttgaaa attatcgcgt aaaaccggca
     3661 agcgtctttc cggaaaattt tgacgttaca gacaagacgc tattattgga gacatcacaa
     3721 cagtggacat tgaattatgg attgtggcgg gcgccacggt cctgtcactt tttttcgggc
     3781 tggcggcttt tactctctcg tcgttcagct ggtccaaact cggcgatacc ctcgccggca
     3841 caaaacaggg caagaaacgc ctcgataccc ttgaaaagca tctcaaaccg cttcagatga
     3901 cgctcgcgat gtgtaagacc attgccaatt tcgtgcttat cgccgccatg gtctgcctga
     3961 tgtcggacaa gacggatttt ccaacgtggc agcaggctgc gatagcaatc gtcatctgca
     4021 gcgtcataat tgcaatattc ggcgtagcca tcccgttcgc atgggccaac tactcgggcg
     4081 aaaaaatgct cgtcgccaca ctgccggtac tgctgggact gcgattcata ctctggccgc
     4141 tggtggcgtt gatgggactg ttcgacctgc cgatacggcg gctcagcggc gtacacgacg
     4201 acgaggaaga gcccaccggc gaagccaagg ctgaaatact ccaagccgcc gcggacggcg
     4261 ccgctgaagg cgctgtagac gccgaagaag tggaaatgat agaatccgtc atggaatttg
     4321 ccgaaacccg cgcggcggaa ataatgaccc caagaacgga tatctttgca ctctcagccg
     4381 acacaccatg ggaagaagca gtcaaaacca tccatatggc cggccatacg cgcgtaccca
     4441 tttacgaaga taacctcgac aacatcgtgg gcattattta cgccaaagat ttgttgaaat
     4501 tcatgggcca tcagccgccc agcgacctga aggctatctg ccgcaaaccc tacttcgtac
     4561 ccgactcgaa atgcctggac aatctgctga aagattttaa ggctcgcaaa gttcacatcg
     4621 ccgtcgttct cgacgaatac ggcggcacgg ctggccttgt gactatagag gatgtacttg
     4681 aagaaatagt aggggaaata tccgatgaat atgacaaaac aaaagacgcc caggtacgcc
     4741 gcataagcca ggactcaatt gacgtcgacg gcagactgcg agtcgatgaa ctcaacgacg
     4801 aacttgatat cgaactgcca gaagatgaaa attacgatac ggtggctgga tttgcggcgg
     4861 catatctggg atacattccg ccggctggcg aaaaattcgt ggcgttcggc gcggaattca
     4921 cg
//
LOCUS       DAONFI010000172         4895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_20469_length_4895_cov_4.473140, whole genome shotgun sequence.
ACCESSION   DAONFI010000172 DAONFI010000000
VERSION     DAONFI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20469_length_4895_cov_4.473140"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PLK08_06470"
     CDS             complement(<1..1034)
                     /locus_tag="PLK08_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53177.1"
                     /translation="MKYYKNIGGNYLLRQAVTAAILPLLFCAGCSVIKEDTTSVIFSE
                     DDSMVGYSWIFQDLWGEMDEGYLKGTTYEYVGFARTDTPQTTYILQTLKKKKTPNFAT
                     ESLVKYVAISPDNKKMAIVSSNAVDIMDIATFKLNRLTMKDERIGTLRWLNAEEVVYT
                     SILPPAKNETVTYKCVFRQYFSSDEGNRVTLFRDLTNLYEQDAFAEFWSPDGKTMLLL
                     GQLHIRLLDVETGRLSNSLLERFGENRGVVWSPDSKRLFIAFGWSWDQKGKCGGILVQ
                     LKPLKVTDYSTQVRQTFYTEPFRVLDWTKDGKYIIGWGMQLQTCLIDPDGWKVFWFEE
                     KYNESLPKYQS"
     gene            complement(1335..2399)
                     /locus_tag="PLK08_06475"
     CDS             complement(1335..2399)
                     /locus_tag="PLK08_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS53178.1"
                     /translation="MNEIILKNVTKKFDAGRIPAVDDVSLEIKPGELFFLLGPSGCGK
                     TTLLRIIAGLVRPDSGRVIIAGRDVTKLNAEKRDTAMIFQNYALWPHMTVAQNVAFGP
                     KMKGVNSEKCASAVENSLRLVEMHKYASRKPNELSGGQQQRVAVARALAADAECLLLD
                     EPLSNLDAKLRANMRLQLRELIKRSNTTAVYVTHDQAEALAMADRIAVMNNGKIVQVA
                     TPQELYSSPSEKFVADFIGEANFIAGRVVKAGETTTVETSCGNIESAATGFVVGQAVT
                     CCVRPECVRLVSPDETADGLPGTMEFRIFTGGGWQFVFSLAQGENWKASIHGRQCDTI
                     EPGTVAMLLADPKDVILLKE"
     gene            complement(2402..4132)
                     /locus_tag="PLK08_06480"
     CDS             complement(2402..4132)
                     /locus_tag="PLK08_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPS53179.1"
                     /translation="MSFGGYIKRIFVEPFGTSLTAGLTALLAAALIYPVCIAVHDAFV
                     TQNGQFSLYWFTRLADDGSLRLLGNSLLLAAITTVACTIISLPLALLRVECEFIGREF
                     FSLMMLAPLILPPFVGAIAIQRFLGQFGSLNLLLDRLGVIDFASGELPPDWLASGFVG
                     VVIMQVLHLFPIIYLNAAASLTNLDPSQVEAAENLGAPAWVRLRRIILPQIRPGLFAG
                     GAIVFIWSLTDIGTPLMLNYRDLAGVRIFSSLTSGKYFGSDFALVVVLLAVSIACYVL
                     GKFVLGREPEQINTRAMRGAVTKRLGAAGTLGAWVLFGTVTVMAILPHIGVVLSALAG
                     DWFDSILPEQYTFAHMAGVVTDSQTYLSIVNSLQYAGCSTVIDIIMGGLIAWLLVRGR
                     TVGSSVLDAMAMLPLAVPGLILAAGYVAMTATGPLAKIGPLGKYPAVILIIAYSVRRL
                     PLVVRGISAGLQQIPTTYEEAARNLGAGRIKTARRITLPLLTSSFAAAAVLAFAFAML
                     EVSDSLVLAQVAENYPITKQIYSLYNSGTGFSANIAAALGVYGMVLLGGTMAISSIIM
                     GRKFGSIFRA"
     gene            complement(4129..>4895)
                     /locus_tag="PLK08_06485"
     CDS             complement(4129..>4895)
                     /locus_tag="PLK08_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPS53180.1"
                     /translation="LADPTKSSSAAAAYKTIYDNNAAAGNGWPTVLAICGNAKKFYDS
                     AGGAADAVISEAPLATVISFYGTMRVSRYPGRLKYISATDMFSADPIGILKNPPHPAL
                     ARAFVNFVLSPGGQAILGCRAGTPVGPAVKDLFMAPIRMDFYTSDFYVRYALPDLPNP
                     YLADTAAGKSSTTGVDYNILKNLIYAAAVMQGDLLKQARKRIIADPVLTTEFYALPDN
                     ALAPQTVIHMQNAVENEKIMTGWHNYWRNKYEKIIR"
BASE COUNT          998 a         1351 c         1342 g         1204 t
ORIGIN      
        1 gactgatatt tcggcagact ttcattgtat ttttcttcga accagaagac tttccagcca
       61 tcagggtcta tcagacaggt ctgcagttgc atgccccagc cgatgatgta tttgccgtcc
      121 ttcgtccagt cgagcactct gaatggttcg gtataaaacg tctgtcgcac ctgggtgctg
      181 taatcggtga ctttcagcgg ctttagctgc acgagtatgc cgccgcactt gcccttctgg
      241 tcccatgacc agccgaacgc gatgaacagc cgcttgctgt cgggactcca cactacgccg
      301 cgattttcac cgaacctctc caaaagcgaa ttcgacagtc tgccggtttc aacgtccagc
      361 agccttatgt gcagctgtcc caacagaagc atggttttgc cgtcgggcga ccagaattcc
      421 gcgaatgcgt cctgctcgta gagattcgtc aaatcgcgga acagcgtcac gcgattgcct
      481 tcgtcggagc tgaaatattg cctgaatacg catttgtacg tgaccgtttc atttttagcc
      541 ggcggcagta tcgacgtgta aaccacttcc tccgcgttca gccatcgcag tgtgccgatg
      601 cgttcatcct tcatggtaag ccggttgagt ttgaacgtgg cgatgtccat tatgtcgacg
      661 gcgttgctgc tgactatcgc catcttttta ttgtcggggc tgattgcgac gtatttgacc
      721 agcgattccg tggcgaaatt cggggttttc tttttcttga gcgtctggag gatatatgtt
      781 gtctgcggcg tgtccgtcct ggcaaagccg acgtattcat acgtcgtgcc cttgagatag
      841 ccttcgtcca tttcgcccca taaatcctgg aaaatccatg aatacccgac catcgaatcg
      901 tcttcgctga aaattacgct ggtggtgtct tctttgatga cgctgcagcc ggcgcagaaa
      961 agcagaggca atatcgccgc cgtcacggct tgcctgagaa gataatttcc gccgatgttt
     1021 ttataatatt tcatctttta tatgttactc aaagtgaccg attgacgata tttaccttta
     1081 cacctagaac ctgtcccaaa aaccttggct gcctgcaatc ggcacttttg cagcctggcg
     1141 gcgtcaggaa aacggcagaa tatttttttt aaaaaaatat gccgctattt ttcttccttg
     1201 ccagcccgca aaattgctcg atttcggcga cgcccatgtt tttgggatag gttctagtaa
     1261 tctggaaacc atcatatcgg tcagtttgaa atatgttatg tctctgcgtg ttatgtatat
     1321 cggctttttt tgcattattc tttgagcaat atgacgtctt tcgggtcagc cagcagcatt
     1381 gccaccgtac ctggttcaat cgtatcgcat tggcggccgt gtatggatgc cttccagttc
     1441 tcgccttgtg cgagtgaaaa tacgaactgc cagccgccgc cggtgaaaat tctgaactcc
     1501 atggttcctg gcaggccgtc ggctgtttca tcgggcgaga ccagccttac gcattcaggc
     1561 cttacgcagc atgtgacggc ttggccgacg acgaaacccg tcgccgccga ctcgatattg
     1621 ccgcatgagg tctcgacggt agtcgtttcg ccggctttta cgacccggcc ggcgatgaag
     1681 ttcgcttcgc cgatgaagtc tgcgacgaat ttttcagacg gcgacgagta caattcctgc
     1741 ggcgtagcga cctggacgat ttttccattg ttcatgacgg ctatgcggtc agccatcgcc
     1801 agcgcttcag cctggtcgtg cgtgacgtag actgccgtcg tattgctgcg ttttatgagt
     1861 tcccgcaact gcagtcgcat atttgcccgc agtttcgcat ccagattgct tagcggttcg
     1921 tcgagcagca ggcattctgc gtcagccgcc agcgcacgtg cgacggctac ccgctgctgt
     1981 tggccgccgg aaagttcatt cggcttgcgt gaagcgtatt tgtgcatttc caccagtcgc
     2041 aggctgtttt cgacggctga tgcgcatttt tcgctgttta ctcctttcat tttcggcccg
     2101 aatgctacgt tctgcgccac cgtcatgtgc ggccacagtg cgtagttctg aaaaatcatg
     2161 gctgtgtcgc gtttttcggc gttgagtttc gttacgtcgc ggccggcgat gattacgcgt
     2221 ccgctgtccg gcctgacgag gccggcgatt atccgcagca gcgtggtttt gccgcagccg
     2281 ctcggcccga gcagaaagaa cagctcgcct ggttttatct ctagactcac gtcgtcgacc
     2341 gcaggtatcc ggccggcgtc gaattttttt gtcacgtttt tgagtattat ttcgttcatg
     2401 ttcacgcccg aaaaattgaa ccgaattttc tgcccatgat tatgcttgaa atcgccatcg
     2461 tgccgcccag cagtaccatg ccgtatactc ccagcgctgc ggcgatgttg gctgaaaagc
     2521 ccgtgcccga attgtacagc gaataaatct gtttcgttat cggatagttt tccgccacct
     2581 gtgccagcac cagcgagtcg ctgacttcga gcatggcgaa tgcaaacgcc agcaccgccg
     2641 ctgcggcaaa actggatgtg agcaggggca gggttatgcg acgggcggtt tttatcctgc
     2701 ctgcgccgag attgcgcgcc gcctcttcat aggttgtcgg tatctgctgc aagccggctg
     2761 atatgccgcg aaccaccagc ggcagacgtc gaacgctgta tgcgattata aggatgacgg
     2821 ccgggtattt gccgagtggg cctattttcg ccagcgggcc ggttgccgtc atggctacgt
     2881 agccggcagc gagtataagg cccggcacag ccagcggtaa cattgccatc gcgtcgagca
     2941 cgctgctgcc gacggttctg ccccgcacga gcagccaggc gattaacccg cccataatta
     3001 tgtcaatcac cgttgaacag cctgcgtatt gcagcgagtt tactatgctc agatatgtct
     3061 gagagtccgt aacgacgcca gccatgtgtg caaaagtgta ttgttccggc aggatggagt
     3121 caaaccagtc gccggctaga gcgctcagga cgacgccgat gtgcggcagt atcgccataa
     3181 ccgtcactgt gccgaacagt acccacgctc ccagtgtgcc agccgcgccg agtcgtttag
     3241 taacagcgcc tcgcatcgcc cgcgtgttta tctgctcagg ttcgcggccg agtacgaatt
     3301 tgcccagtac gtaacaggcg atggataccg ccagcaatac caccaccagt gcaaaatcgc
     3361 tgccgaaata tttgccgctg gtcagtgaac tgaagatacg cacgccggcc aggtcgcgat
     3421 agttgagcat tagcggcgtg ccgatatcgg taagcgacca tatgaataca atcgcaccgc
     3481 cggcgaagag gccgggccgt atctgcggca gaattatccg tcgcaatctc acccacgccg
     3541 gcgcgccgag attctccgcc gcctcaacct gcgacgggtc gagattcgtc agagatgcgg
     3601 ctgcattcag atagattatc gggaacagat gcagtacctg catgattacc actccgacga
     3661 agccgcttgc cagccagtcc ggcggcagct cgcctgaagc gaaatctatc acgccaagtc
     3721 tgtccagcag cagattcagc gagccgaact ggcccagaaa ccgctggatg gctatcgcgc
     3781 cgacgaacgg cggcagtatt agcggggcaa gcatcatcag agaaaaaaac tccctgccga
     3841 taaattcgca ttccaccctc agcaacgcca gcggcagtga tattatcgta caggcgacgg
     3901 tcgttatcgc ggcgagcagc agcgagttgc ccagcagccg caaactgccg tcgtcggcta
     3961 aacgtgtgaa ccagtataaa ctgaattgcc cgttctgcgt gacgaacgcg tcgtgcaccg
     4021 ctatgcagac cgggtatatc aacgctgccg caagcagtgc cgtcagcccc gcggtcagcg
     4081 atgtgccgaa tggttctacg aatatgcgtt ttatgtagcc gccaaagctc atctgattat
     4141 tttctcgtat ttgttccgcc agtaattatg ccaaccggtc atgatttttt cgttctcgac
     4201 ggcgttctgc atatggatga ctgtctgcgg ggctagcgca ttgtccggca gggcgtagaa
     4261 ttccgtcgtt agtacggggt cggcgatgat gcgcttgcgg gcctgtttga gcaggtcgcc
     4321 ctgcatgacg gcggcggcat agataagatt cttcaaaatg ttgtagtcga cgcctgttgt
     4381 tgatgatttt ccggcggctg tgtcggcgag atacggattc ggcaaatccg gcagggcgta
     4441 tcggacataa aaatcgctgg tgtagaagtc catgcgtatg ggggccatga agaggtcttt
     4501 gacggctggg ccgaccggcg taccggcgcg gcagcccagg atagcctggc cgccaggaga
     4561 cagcacgaaa ttcacaaacg cgcgggctaa cgcaggatgc ggcggatttt taagtatccc
     4621 tatcgggtcg gcggaaaaca tgtcagttgc ggagatgtat ttcagccgcc ctggataacg
     4681 gctgactcgc attgtgccat aaaaacttat taccgtcgca agcggggctt cagagattac
     4741 ggcgtcggct gctccgccgg cgctgtcgta gaatttcttt gcgttgccgc agatggcaag
     4801 taccgtcggc cagccattgc cggcggcggc gttattgtcg tatatcgtct tgtaggctgc
     4861 cgccgcggaa ctggacttcg tggggtcggc tagcg
//
LOCUS       DAONFI010000173         4859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_20641_length_4859_cov_4.475645, whole genome shotgun sequence.
ACCESSION   DAONFI010000173 DAONFI010000000
VERSION     DAONFI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20641_length_4859_cov_4.475645"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLK08_06490"
     CDS             <1..475
                     /locus_tag="PLK08_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53181.1"
                     /translation="FEYQFWIYRANSDASAVALLENSETIATGSGKLNTSVYIQEGNI
                     MVHPGGTWVDTNADIAVGVWTRVRMSFDMSSGDYGAYTLYIMPEGQAETEVWTGDLTT
                     SARTVLNAIRFNPQGTTGGQTYIDDISLTIVPEPTAMMLLGLGSIGVLIGRKRHV"
     gene            complement(728..2623)
                     /locus_tag="PLK08_06495"
     CDS             complement(728..2623)
                     /locus_tag="PLK08_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPS53182.1"
                     /translation="MGLEKKLGFLDVFCLASGAMISSGLFVLPGQAYMAAGPAVVLAY
                     AIAGLIMIPALLSQCELATAMPKSGGSYFFVERSMGALPGTFAGLAGWVCLALKSAFA
                     MIGIGAFARLIWPDAPLDAGQWEWLIKIIAVTFCVIFAAMNCISVKMASWTQLVMVFG
                     LLGVLAIFIILGFPAVNQHPNFDHFTAKGFGNILATAGLVFISYGGLTKAANVAEEVR
                     RPGRNLPRAMLTAFWVVTILYVLTVFVVVGTLSSNKLAGEGQVNLTPVSSAAETFMGP
                     AGVILLSVAAILAFVTTGNGGILAASRYPLAMSRDGILPQWMQRVSHRFGTPWVSVLL
                     TAGFMIVTILMLSIEELVKSASTMLLLLFVLINISVLIMRSSRIQNYRPRYLSPFYPW
                     VQIAGVIIYVVIIVQLTAEFGLEPLMTVAAFVIFGVLWYWFYVRPRSTRQSALYYMVQ
                     RIVSPSIRRAGLEQELRTIATQRDDIVHDRFDMLIEKCAILDLQGPISRDELFSKAAA
                     VLSGRLGMAGDEIKRRLEKREEMSSTMIHPGLAIPHIIIDGEKQFDILLVRCRQGVVF
                     DAEQPYVKMIFILVGSADERNYHLRALMAIANIVEEQEFVHNWLEAEDIEHLRDLILL
                     SNRNRGA"
     gene            2866..4290
                     /gene="tig"
                     /locus_tag="PLK08_06500"
     CDS             2866..4290
                     /gene="tig"
                     /locus_tag="PLK08_06500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPS53183.1"
                     /translation="MSEEPTATEETQEEKTPISVVVEDSGTLKKKVTVTVPADRITAK
                     RDELYGELNTSAVIPGFRVGHAPRRLLEKRFGKEVGTDVRNGLVGDSLGEAIEDAELK
                     TLGEPELKIDDIVIPEQGDLEYSFEVEVEPEVEMPELDGIELKKETETVDEAKIDDYI
                     ENIRQGQARYTEAAESEPAQEGDSIVASAKITGDGIEFENPRVMLRVAPSYVEGIALP
                     EAANDLLGKKTGDVVTIKTTVPESHAKEDWQNKEVSIELTVNKVSHRHVPELNEEFAK
                     QMGFDSIAELKKYVGERLEANLESQTQQKLRQQVCDYLLEKTSFEMPEGLLKRQTAYT
                     LQRQVINLMQMGVPQEKVLERKAEMESSAREEAHKSLRLSFILGKVAEKNKTEVNESE
                     INSRVAQIAMQYGRRPERMRQELAADGSLGQVAQALLEEKVIDSILETAKIEEVAAAK
                     AEKKPAAKKAAKKTAKKAEKKDAE"
     gene            complement(4414..>4859)
                     /locus_tag="PLK08_06505"
     CDS             complement(4414..>4859)
                     /locus_tag="PLK08_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPS53184.1"
                     /translation="EMPARIEEVHHAEEEGITFDFLVAPLEILGNDKGWVRAVRMQRM
                     ELGSPDASGRRRPVPIQGSEFELETDVVVVAIGVRANPLLTAACPELKLNKWGNIEVN
                     ESGMTSVDGVFAGGDIVRGGATVILAMGDGKNAAKNIDEYLKNKK"
BASE COUNT         1310 a         1279 c         1202 g         1068 t
ORIGIN      
        1 ttttgaatat caattctgga tttatcgggc caatagtgat gcttcagcgg ttgcattact
       61 ggaaaatagt gaaactattg caacaggctc tggcaagctt aatacgagtg tgtacatcca
      121 ggaaggaaat attatggtcc accctggtgg aacttgggtt gataccaatg cggacatagc
      181 tgttggagta tggacgcgtg ttagaatgtc gtttgatatg tcttcaggtg attatggcgc
      241 ttatactttg tatatcatgc ccgaagggca agctgaaact gaggtatgga cgggtgattt
      301 gacaacatcg gctcggacag tgttaaatgc aattcgtttt aatccccagg gaacgactgg
      361 cgggcagaca tatattgatg atatctccct gacgatagtt ccagagccga cggctatgat
      421 gcttcttggt ttaggcagca ttggcgtgct gattggtcga aaacggcacg tataatcaaa
      481 agtgctcaaa ctaattctat gaaggtgatt aaaccccgat tggctctggc atgtaatatg
      541 ttgaaaccaa taacggccta atgccggtgt gcatattgca tatttgtggc atgcttttta
      601 aagaaaattg gcctgaagaa tttgacaatt tctggcagca ggctggtatt ttgataccag
      661 acaaaatctc tggcccacat tcaatttgta atcagacaag gtgttatgga tggtttctat
      721 gttctactca agcccctctg tttctgttgc tcagcaatat caggtcgcgc aggtgttcga
      781 tatcctccgc ctccagccaa ttatgaacga actcctgttc ttcgactatg ttggcgatag
      841 ccatcagcgc acggaggtga taattccgct cgtcggcact gccgaccaat atgaaaatca
      901 ttttcacgta aggctgttcc gcgtcaaaca cgacgccctg tcggcagcgg acaaggagaa
      961 tgtcaaactg cttttcaccg tcgataatta tgtgtggaat ggcgaggccc ggatgtatca
     1021 tcgtgctgga catttcctct cgtttttcca ggcggcgctt aatttcatcg ccagccattc
     1081 ccaggcggcc ggataaaaca gccgcagcct tcgaaaacag ttcatccctg cttatagggc
     1141 cctgcaggtc gagtatggca catttttcta taagcatgtc aaagcggtcg tgaacgatat
     1201 cgtcccgctg tgttgcgatg gtacgcaact cctgttccaa gccggctcgc cggatactcg
     1261 gcgaaactat tcgctgtacc atataataca gagcgctctg gcgggtgctt ctcggcctga
     1321 cgtaaaacca gtaccacaac acgccgaaaa ttacgaacgc cgccacagtc atcagcggtt
     1381 caaggccgaa ctcagcggta agttgcacga ttatcaccac gtagataata acgccggcta
     1441 tctgcaccca cggataaaac gggctgagat accgcgggcg ataattttgt atgcggctgc
     1501 tgcgcattat aagaacggat atgtttatta gcacgaaaag cagcagaagc atggtcgagg
     1561 ccgacttgac gagctcctct atgctcagca tgagtattgt cacaatcata aagccagccg
     1621 tcagcagcac cgaaacccac ggcgtgccga agcggtgtga aacccgctgc atccattgcg
     1681 gcagtatgcc gtcgcggctc atcgccagcg gataccgcga cgccgccaat atgccgccgt
     1741 tgcccgtcgt cacgaacgcc agtattgccg caactgacaa cagtatcacg ccagccggtc
     1801 ccataaatgt ctcagccgcc gaacttaccg gcgtcagatt cacctgccct tcgccggcaa
     1861 gcttattcga agaaagcgtt cctactacaa caaataccgt caagacataa agtatagtaa
     1921 cgacccaaaa agcagtgagc attgcgcgag gcagattgcg gccgggccgc cggacttctt
     1981 cggctacgtt ggcagccttg gtcaaaccgc cgtatgaaat gaacacaagg ccagccgtag
     2041 cgagaatgtt gccaaaaccc tttgcggtaa aatggtcgaa attgggatgc tggttgacgg
     2101 ctggaaatcc caagattatg aatatcgcca acacgcccag caagccgaaa accatcacaa
     2161 gctgcgtcca gcttgccatc ttgacgctga tgcaattcat cgcggcaaat atcacgcaga
     2221 acgtcacggc gatgattttt atcagccatt cccattggcc ggcgtcaagc ggcgcatccg
     2281 gccagattaa tcttgcgaac gctcctatgc caatcatggc gaacgcactc ttgagagcca
     2341 ggcaaaccca ccccgccaag ccggcgaacg tgcccggcag agcacccatg ctgcgttcga
     2401 caaaaaaata actgccgccg ctcttgggca tcgccgtcgc caattcacat tggctgagca
     2461 atgccggaat cattatcaaa ccggcaatcg catacgcaag cactaccgca ggcccggcag
     2521 ccatatatgc ctggcccggc agcacaaaca ggccggaact tatcattgcg ccagacgcca
     2581 gacagaacac atccagaaaa ccaagttttt tttccaagcc cattattttt gacccttatt
     2641 tgtgacctta attttagtta cagccttcac ttgcgatatt ttatctacct gaaccgcata
     2701 attcaccgaa aaaagaacta taatgttaca aatagattga cttaaatcct ttttttgact
     2761 atatttcttc cccttgaaca gccttccgcc aagtgcgggg cctgccgccg gggatgtgtc
     2821 gcgttggcac atctggtcgc gacgggctaa tctggagtat gacaaatgtc agaagaaccc
     2881 acagccacag aagaaaccca ggaagaaaag acccctatca gcgtcgtagt agaagattcc
     2941 ggcacgctga agaagaaagt cactgtcacc gtaccagccg accgcatcac tgccaaacgt
     3001 gatgaacttt acggcgagct gaacacgtcg gctgtaattc caggttttcg cgtcggccat
     3061 gcacctcgca ggctgctgga aaaacgattc ggcaaggaag tcggcacaga cgtacgtaac
     3121 ggccttgtcg gcgactcact cggcgaagcc atcgaagacg ctgaactcaa aaccctcggc
     3181 gagccggaac tgaaaatcga cgatatcgtc atacccgaac agggcgacct tgaatattca
     3241 tttgaagtcg aagttgagcc ggaggtcgaa atgccggaac tcgacggaat tgaactcaag
     3301 aaggaaactg aaaccgtcga tgaggcaaaa atcgacgatt atattgaaaa catccgccag
     3361 ggccaggccc gctacaccga agccgcagaa agcgaaccgg cacaggaagg cgacagcatc
     3421 gtagcctcgg caaagattac cggcgacgga atcgaatttg aaaatccgcg ggttatgctc
     3481 cgcgtcgcgc cgagttacgt agagggcatc gccctgccgg aggcggcgaa tgacctgctc
     3541 ggcaaaaaaa ccggcgacgt ggtgaccatt aaaaccaccg ttccggaaag tcacgcgaaa
     3601 gaagactggc agaacaagga agtatccatc gaactgacgg tcaataaggt atcgcaccgc
     3661 catgtgccgg aactcaatga agaatttgca aagcagatgg gtttcgacag catcgctgaa
     3721 ttgaagaaat acgtcggcga acggctggaa gccaaccttg aatcgcagac gcagcagaaa
     3781 cttcgtcagc aggtctgtga ttatctgctt gaaaagacgt ctttcgaaat gcccgaaggc
     3841 cttctcaagc ggcagaccgc ctatacgctt caacggcagg tgataaacct catgcagatg
     3901 ggtgtgccgc aggaaaaagt tctcgaacgc aaggctgaga tggaatcctc cgcacgggaa
     3961 gaagcacaca agtcactgcg gctgagcttc atactcggca aagtggcgga aaagaacaaa
     4021 accgaagtca acgaaagcga aatcaattct cgcgtcgcgc agatagccat gcagtatggc
     4081 cgtcgtcctg aacgcatgcg tcaggaactc gcagccgatg gctcacttgg ccaggtcgca
     4141 caggcactgc tggaagaaaa agtcatcgac agcattctgg aaaccgccaa gatagaagaa
     4201 gtggctgccg cgaaagcgga aaagaaaccg gctgcgaaga aagccgcaaa aaagaccgca
     4261 aaaaaggctg agaaaaaaga cgctgaataa ttatttcccc ttccggaatt acaggcaata
     4321 aaaaagaggc tgggcataaa acccgacctc tttttttatt gcgttttctg aaaatagagc
     4381 agttctccaa aaattttttt gaaacaactc gatttatttt ttatttttca gatattcgtc
     4441 gatatttttt gcagcatttt tgccgtcgcc catagccaga atgaccgtcg cgccgccgcg
     4501 gacgatatcg ccgccggcaa agacgccatc aaccgacgtc atgccgcttt cgttcacttc
     4561 gatattgccc cacttattaa gcttcagctc agggcaggct gccgtcaaca gcgggttggc
     4621 tcttacgccg atggctacca ccacgacgtc tgtttccagt tcgaattcac tgccctgaat
     4681 cgggaccggc cgtcgacggc cggaggcatc cggcgaacca agttccatgc gttgcatgcg
     4741 taccgctctt acccagcctt tatcattgcc gagtatttcg agcggcgcga cgaggaaatc
     4801 aaacgttatg ccttcctctt cggcgtgatg cacttcttcg attcgagccg gcatttccg
//
LOCUS       DAONFI010000174         4831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_20806_length_4831_cov_4.130235, whole genome shotgun sequence.
ACCESSION   DAONFI010000174 DAONFI010000000
VERSION     DAONFI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20806_length_4831_cov_4.130235"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2844)
                     /gene="carB"
                     /locus_tag="PLK08_06510"
     CDS             complement(<1..2844)
                     /gene="carB"
                     /locus_tag="PLK08_06510"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPS53185.1"
                     /translation="MPKRTDIRKILIIGAGPIVIGQACEFDYSGVQACKALRDEGYEV
                     VLLNSNPATIMTDPEMAEHTYIEPITLEILERILEKERPDAILPTLGGQTALNLASSA
                     YKTGLLRKYDVEMIGATAEVIKRAEDRDEFKEIVTAEGIEVLRSEQVENMDEARKVIK
                     DLGLPVVIRPSFTLGGTGGSIAYNYDDFEKKCKSGLEISPVHSVLLEECAVGWKEFEL
                     EVMRDKANNSVIICSIENLDPMGVHTGDSITVAPIQTLTDREYQAMRDEALVIIKAIG
                     VETGGCNIQFAVHPQTGRRVAIEMNPRVSRSSALASKATGFPIAKIAAKVAVGMTLDE
                     IPNDITLETPACFEPTIDYCVVKIPRFTFEKFPASDPTLTVSMKSVGEVMSIGRTFKE
                     AFQKALRGLETKRFGLGADGRDVNLDDIDDSMIEEKLSIPNAERVFWLRYAFQKGYSV
                     DDVYGLTMIDPWFLDNIKQIVEYESTLAGKASLTDKELFDAKRMGFSDVQLAHLLGKT
                     QDEIREQRYDADIRAVFKQVDTCGGEFEAHTPYFYSTYEKTDESESKGGGIMILGGGP
                     NRIGQGLEFDYCCVHAADAITNAGFESIMVNCNPETVSTDYDTSDRLYFEPLTLEDVL
                     EIVNREKPRGLIVQFGGQTPLNLSSGLKKCGVPILGTSPDSIDRAEDRKLFSEMVRKL
                     NLRQPANGMADTVQGSLDIAAQIGYPVIVRPSFVLGGRAMEIVYSPEDLKVFAKMAFE
                     TSDGAAVLIDKFLEDAIEVDVDAVSDGTDVYIAGLMEHIEYAGCHSGDAAMVLPPQQL
                     PQSVIDEISDATRKLAFELDVRGLMNVQFAVSQDNEVYILEVNPRASRTVPFVSKVTG
                     VPIAKVATHIMLGSTLKEMGLDKPLPPLNHMGVKESVFPFNRFPGVDAVTAVMNLDIL
                     GIAAATTSACATAKQEPPHVLTAMGLSSEQALSS"
     gene            complement(2921..4534)
                     /locus_tag="PLK08_06515"
     CDS             complement(2921..4534)
                     /locus_tag="PLK08_06515"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPS53186.1"
                     /translation="MSKHIFITGGVVSSLGKGITAASLGLLLKQRGLKVAMQKFDPYL
                     NVDPGTMNPYQHGETYVTRDGAETDLDLGHYERFTGIETTRNSNYTSGRIYSSVIAKE
                     RRGDYLGGTVQVVPHITDEIKNAVRSVTDSVDVVITELGGTVGDIEGLPFLEAIRQFG
                     LQEGRDNVVFVHVTLIVNVVAAGERKTKPTQHSVQKLREIGIIPDFLVCRCNAPLNDD
                     MRRKIALFTNVSEDCVIDEPDVKDTIFEVPFQFVDQQLDMKIIKRLGLNAKNLDMSEW
                     KTIIERIRHPKGELTVAVVGKYGELQDAYKSIYEALDHGGIANEYKVKIKRVLAEKVE
                     AGRVEDFLKDVDAVLVPGGFGNRGMEGKIAACRYARENGIPFLGLCLGLQAAAIEFAR
                     NVCGLEGANTTEIDEKTPHPVIYIMEDQKHITDKGATMRLGNWPCRVIDGTKARAAYG
                     EEMILERHRHRYEFNNRYRKIFEEKGMVFSGMNPDKEIVEIIELRDHPWFVATQAHPE
                     FRSTPVRPHPLFVKLVENAIKYKQDKKKA"
     gene            complement(4560..>4831)
                     /locus_tag="PLK08_06520"
     CDS             complement(4560..>4831)
                     /locus_tag="PLK08_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HPS53187.1"
                     /translation="QPVKDLRTGQVAITVQNHGFCVDMDSLPADDVQVTHINLNDNTL
                     EGFEHKKFPVFCVQYHPEASPGPHDARYLFKRFASLMDEYRKTKL"
BASE COUNT          936 a         1288 c         1315 g         1292 t
ORIGIN      
        1 cgaggacagg gcctgttcgg aggagagccc catcgcggtc agcacgtggg gcggctcctg
       61 cttggcggtg gcgcaggccg aagttgttgc cgctgcgatt ccgagaatgt ccagattcat
      121 caccgccgta acggcgtcga cgccagggaa gcggttgaac gggaataccg attccttgac
      181 gcccatatgg ttgagcggcg gcagcggctt gtcaaggccc atctccttca gcgtgctgcc
      241 gagcatgatg tgcgtcgcca ccttggctat cggcacgccg gtgaccttgg agacgaacgg
      301 cacggtacgc gacgctcgcg gattgacttc gagtatgtag acttcgttat cctgtgagac
      361 ggcgaactgc acgttcataa ggccgcgcac gtcgagctca aatgcgagtt ttcgcgtcgc
      421 gtctgatatt tcgtcaatta ccgattgagg gagctgctgt ggcggcagaa ccatggcggc
      481 gtcgccgctg tggcagccgg cgtattcgat gtgttccata aggccggcga tgtaaacgtc
      541 cgtgccgtcg cttacggcgt cgacgtcgac ctctatggcg tcttcaagga atttatcgat
      601 gagaactgcc gcgccgtctg aagtttcaaa cgccattttc gcaaaaactt tgaggtcttc
      661 cggcgaatag acgatttcca tcgctcggcc gccgagtacg aaactcggcc gaactattac
      721 gggatatcct atctgtgcgg cgatgtcgag cgagccctgt accgtgtcag ccatgccgtt
      781 cgccggctga cggagattga gtttgcgaac catttcggag aaaagtttgc ggtcttcggc
      841 gcggtcgatg gagtcggggc ttgtgccgag tatcggaacg ccgcactttt taaggcctga
      901 cgacaggttc aacggcgtct ggccgccgaa ctgcacgata aggccacgcg gcttttcgcg
      961 attgactatc tccaacacgt cctccagcgt caacggctcg aagtacagcc tgtcggatgt
     1021 gtcataatcg gtcgaaaccg tttcagggtt gcagttgacc attatggatt caaagccggc
     1081 gttggtgatg gcgtcggcgg catgtacgca gcagtagtcg aattcaaggc cctggccgat
     1141 gcggttcggc ccgccgccca gaatcattat cccgccgcct ttggattcgg attcgtcggt
     1201 tttttcatac gtcgaataga aatacggcgt atgagcttca aattctccgc cgcaggtgtc
     1261 gacctgtttg aagacggcgc gaatatcggc gtcgtagcgc tgttcgcgaa tttcatcctg
     1321 cgttttccca agcagatgcg caagctgaac gtcgctgaaa cccattcgct tggcatcgaa
     1381 aagttccttg tcggttagcg atgccttgcc ggctaacgtc gattcgtatt cgactatctg
     1441 cttgatatta tccagaaacc acgggtctat catggtcagc ccatacacgt cgtccaccga
     1501 gtaacccttc tggaacgcat atcgcagcca aaaaactctt tctgcattcg ggatgctgag
     1561 tttttcctca atcattgaat cgtcgatatc gtcgaggttg acgtccctgc cgtccgcacc
     1621 taagccgaac cgcttggttt caaggcctct caacgccttc tggaacgcct ctttgaacgt
     1681 tcggccgatg ctcattacct cgcctacgga cttcatgctg accgtaagag tggggtcgga
     1741 cgccggaaat ttttcaaacg tgaatcgtgg tatttttacg acgcagtagt ctatcgtcgg
     1801 ctcgaaacac gccggtgttt ccagcgtgat gtcgttcggg atttcatcca gcgtcatgcc
     1861 tactgctacc ttagccgcta ttttggctat cggaaaaccg gtagccttcg atgccagcgc
     1921 cgacgaacgg ctgactcgcg gattcatttc gatggctacg cggcggccgg tctgaggatg
     1981 aacggcaaac tggatattgc agccgcccgt ctcgacgccg atagccttga ttatcaccag
     2041 cgcctcatcc cgcattgcct gatattcacg gtcggtgagc gtctgaatcg gggcgacggt
     2101 tatggaatcg ccggtgtgaa cgcccatagg gtcgaggttt tcaatcgagc agattatgac
     2161 ggaattattg gctttgtcgc gcatgacttc gagctcgaac tctttccagc cgacggcgca
     2221 ctcttccagc aatacgctgt gaaccggcga aatctccagc ccgcttttgc atttcttttc
     2281 aaagtcgtca tagttatacg ctatgcttcc gccagtaccg ccgagcgtaa aacttggacg
     2341 tatgactacc ggcaggccca ggtcttttat gactttgcgg gcttcgtcca tgttctcgac
     2401 ctgttcgctg cggagaactt ctatgccttc agccgtgacg atttccttga attcgtcgcg
     2461 gtcttcggca cgcttgatga cttccgcagt tgcgcctatc atttctacgt cgtacttccg
     2521 cagaaggccg gtcttgtatg cggacgaagc gagattcagc gcagtctggc cgccgagggt
     2581 cggcaatatg gcgtcgggac gttccttctc caggattcgt tcgagtattt cgagcgttat
     2641 gggctcgata tatgtatgtt ccgccatttc ggggtcggtc atgattgtgg ctgggttgga
     2701 gttcagaaga accacttcgt agccttcgtc gcgcaaggct ttgcacgcct ggacgccgga
     2761 gtagtcaaac tcgcaagcct ggccgattac tatcgggcct gcgcctataa tcaatatctt
     2821 gcgtatgtcg gttcttttgg gcatatcgta ttccgttcaa gttctgattc ttacgatttt
     2881 tttcaatttt tttttaagat aaattagtca ttggtcaaag ttaggccttt tttttgtcct
     2941 gcttgtattt tatggcgttt tcaaccagtt tcacgaaaag cggatgcggc cgtacaggcg
     3001 tagagcggaa ttccggatgt gcctgcgtgg cgacaaacca gggatggtct ctcagttcga
     3061 taatttccac tatttcctta tccggattca tgccgctgaa gaccatgcct ttttcttcga
     3121 aaattttgcg gtatctgttg ttgaactcgt acctgtgccg atggcgttcg agaatcattt
     3181 cttcgccgta ggctgcgcgg gcttttgtgc cgtcgataac cctgcacggc caattcccaa
     3241 gccgcattgt cgcgcccttg tcggttatat gcttctggtc ttccattatg tagatgactg
     3301 ggtgcggggt tttttcgtct atttccgtgg tgttggcgcc ttcgaggccg cagacgttgc
     3361 gggcgaactc gatagccgcc gcctgcaagc ccagacataa tcccaggaaa ggtatgccgt
     3421 tttcacgggc ataacggcag gctgctattt taccttccat gccgcgattg ccgaaaccgc
     3481 ctggcacaag aacggcgtcg acatccttga gaaaatcctc gactctgccg gcttcgactt
     3541 tttccgccag aacacgtttg attttcactt tgtattcgtt ggctattccg ccgtggtcga
     3601 gcgcttcgta tatgcttttg tacgcatcct gcagttcgcc gtatttgccg acgacggcta
     3661 ctgtgagttc acctttggga tgacgtatgc gttcaatgat ggttttccat tcggacatgt
     3721 caagattttt ggcgttaagg cccagccgtt tgataatctt catatcaagc tgctggtcga
     3781 cgaattggaa cggcacttca aaaatcgtgt ctttcacatc cggctcgtct atgacgcaat
     3841 cttcgctcac attagtaaaa agagcgattt tccgtctcat atcgtcgttt agcggggcgt
     3901 tgcagcggca gacgaggaag tccggaatta tgccgatttc acgcaatttc tgcacgctgt
     3961 gttgagtggg ttttgtctta cgttcgccgg cggctaccac gttcacgata agcgttacgt
     4021 gtacgaacac gacgttatcg cggccttcct gcaggccgaa ctggcgaatc gcttccagga
     4081 acggcaggcc ttctatgtcg cccacagtgc cgccgagttc ggttatcacg acgtctacgc
     4141 tatcggtcac gctgcggacg gcgtttttaa tctcatcggt gatgtgcggt acgacctgaa
     4201 cggtcccgcc cagataatcg cctcgccgct ccttggcgat gacggacgaa taaatgcggc
     4261 ccgacgtgta gttgctgttg cgtgtggttt ctatgccggt gaatcgctca tagtgaccca
     4321 ggtcgaggtc agtctccgca ccgtctctgg ttacgtacgt ttcgccgtgc tgatatgggt
     4381 tcatcgtgcc cgggtcgacg ttcaggtaag ggtcgaattt ctgcatggcc acctttaggc
     4441 cgcgctgttt tagcagcagg ccgagcgagg ctgcggttat gcccttgccc aacgaagaaa
     4501 ccacgccgcc tgttatgaat atgtgcttcg acatttttcc gattcccaat cctttggttt
     4561 tataatttcg ttttcctgta ctcatccata agtgacgcga aacgtttgaa aagatatctt
     4621 gcgtcatgcg ggcctggaga ggcttcggga tgatactgca cgcagaatac agggaatttt
     4681 ttatgttcaa aaccttcgag cgtattgtcg ttgaggttta tgtgcgttac ctgaacgtcg
     4741 tcagccggca gcgaatccat atccacgcag aagccgtgat tctggacggt gatggcgacc
     4801 tggccggtgc ggaggtcttt taccggctga t
//
LOCUS       DAONFI010000175         4829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_20816_length_4829_cov_4.308546, whole genome shotgun sequence.
ACCESSION   DAONFI010000175 DAONFI010000000
VERSION     DAONFI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20816_length_4829_cov_4.308546"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..2516)
                     /locus_tag="PLK08_06525"
     CDS             complement(132..2516)
                     /locus_tag="PLK08_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPS53188.1"
                     /translation="MKTLLRIFLAAGVFLSFASSGLADKSPDLTVVEIKVEGNKTTSR
                     NAVLSYIKIREGMPYSEDAVRADRDRLMQSGRFDAADVKSTVTDKGVVLTFVVSERPE
                     ISRLMIEGNRHFSEEELLKDIPFAQGDPLNPANIEQGKLALLNKYRGEGYYLADVKVN
                     RVMKDGGYQVIYSIEEGPQSIITKVRFDGNHYFSKASLIMRTQTKAKLFPFIKGYLNM
                     EKIDRDIISIRNAYVQEGFLDAEVGRELEFSADKSKVTVVFKINEGPRYRVNEIIFKG
                     NKVYSGEQLLEQIKFHRESFFTGETLQIDVKSIKAAYGEIGYIDADIIAHKQFISPDS
                     PAPEWARNIDGGSPALLNLIFEIDEKDRYQIGAIRIQGNDVTQERVIRRELRFYPEER
                     CDTVAIERSKVRLQELQIFKSVEIKPVSVPDPGNPDKFIPGVKDIVVEVEEGKSSNFI
                     AGVGVSTNNGLLGTVSLTYRNFNIMDWPKTWADFIKARSFKGAGQTFQLTAEPGTELM
                     RFTASWFTPYIFDQPYSVGTKAYYFTRDYDRYDVRRLGLQTSLGHTFKNRWAGEVSVN
                     YENVNEDADSDACQEIWDDRGDYNFVRFRGALVRDRTDSRWDPSTGDRFSFSYEQTAG
                     TATFGKFDANYRIYRTLYVDPLDRKHILSGRIAYGQMVGDDIPVFEKYYGGGIGSIRG
                     FEYRGISPRGHYRGTDIIKKNDPIGGDMMFYAGSEYTFPLIGENLRGVLFIDSGTVEE
                     SCSFTNYRISIGAGLRIKIPFMGPVPMALDFGIPVMKAEYDDTQILSFSVGWSF"
     gene            complement(2842..3753)
                     /locus_tag="PLK08_06530"
     CDS             complement(2842..3753)
                     /locus_tag="PLK08_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPS53189.1"
                     /translation="MNRPFPKPAFELDARQQPHREELTLGDGYVTSVYVHDEPAGVSA
                     DIPVVYMHGIQSHPGWFYRSARTLADAGHRVFQVTRRGSGDNSIKRGHCKNPRQLFDD
                     IDCAIDFALKTTGKGKAILLGVSWGGKLICAYALSDAARRQKTAKLVLIAPGLFSLID
                     VPAAKKLAIAACLMTGLRKTTFNIPLGDAELFTANPAMQKYLLDDEFQLHGATAKFMF
                     TSRLLDLAIRKASAGSLDIPVDTILSSNDRIIDNAATRRWLCRVCGSENNIRTLRGEH
                     TLEFESNATEFLQAATDACRTKNPEKS"
     gene            complement(3764..>4829)
                     /locus_tag="PLK08_06535"
     CDS             complement(3764..>4829)
                     /locus_tag="PLK08_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPS53190.1"
                     /translation="LLLLIVGLSRLTHSSSFLIRPKNGAYRWINMGFAELQPSELAKL
                     AEILLLAWYLRLGSHYSKLAGLSVPFILTFVPLFLILIEPDLGTSILLIPVLFTMLFM
                     AGAKMSHLGGIIICTIIVMFFPIPTHTQDMGEKELDSRKATAYWVSPSGDMIVSAAPL
                     AVMEFHQLQRIDGWLRQNDPEIEKNKGYQLSRSKITLGCGQLSGRGDWDDADFYFRTL
                     PEDHTDFIFSIIGGQWGFIGCTAVLTLYLVIIILGVEIAASTDEPFGRLLAIGVISLF
                     VSQIVINTAMTMGLLPITGMTLPLISYGGSSLLVNCAAIGLLVNVAGRKPIMLSRKPF
                     EHSDDYGVAPYRPLEFPPGP"
BASE COUNT         1042 a         1218 c         1317 g         1252 t
ORIGIN      
        1 ctgcataatt gccactcatt tgcgtctcac agtttaattg agaacgccct ctagtattct
       61 ggaaaccgtt aaatcggtca gtttgaatac ttttaaaata aatcagccgt tcaaatgttg
      121 tcttttcgtt tttaaaaact ccatccgaca gaaaagctca gaatttgcgt atcgtcatat
      181 tcggctttca tgaccggtat gccgaagtcc agcgccatgg gtacaggccc catgaatggg
      241 attttgatgc ggaggccggc acctatcgat atgcgatagt tcgtaaacga gcagctttct
      301 tcgactgtgc cggaatcgat gaacagtacg cctctgaggt tttcgcctat cagcgggaag
      361 gtatattcac tgccggcgta gaacatcatg tcgccgccga tggggtcgtt cttctttatt
      421 atgtcggtgc cgcgataatg gccgcgcggg ctgatgccgc gatattcaaa tccgcgtatc
      481 gaaccgatgc cgccgccgta gtacttttca aatactggaa tgtcgtcgcc gaccatctgg
      541 ccataggcga tgcggccgga cagaatgtgt ttgcggtcga gagggtcgac atatagcgta
      601 cgatagattc tgtagtttgc gtcgaattta ccgaacgtag ccgtgccggc cgtctgttca
      661 taactgaagc tgaaacggtc gccggtggac gggtcccagc ggctgtcggt acggtcacgc
      721 accaatgcgc cgcggaatct tacgaagtta taatcgccgc gatcgtccca tatttcctgg
      781 caggcatcgc tgtcggcatc ttcattgacg ttttcgtagt tgacggaaac ttcgccggcc
      841 cagcgatttt tgaacgtatg gccgaggctt gtctgcaggc caagacgtct gacgtcatag
      901 cggtcataat cgcgggtgaa gtagtatgcc tttgttccga cggaataagg ctgatcaaag
      961 atatatggcg taaaccacga tgcggtgaaa cgcatgagtt ctgtgccggg ctcagccgtc
     1021 aactggaacg tctggcctgc gcctttgaaa ctcctggcct tgatgaagtc tgcccaagtt
     1081 ttgggccagt ccattatgtt gaagtttctg taggtcaatg aaacggtgcc cagaaggccg
     1141 ttattggtac taacgcctac gccagctatg aagtttgacg acttgccttc ttcaacttca
     1201 acgacgatat cctttacgcc gggaatgaac ttatcgggat tgcccgggtc aggcacgctg
     1261 acgggcttga tttccaccga tttgaagatt tgcagttcct gcaggcggac cttcgaccgt
     1321 tcgatggcga ccgtgtcgca gcgttcttcg ggatagaacc gcagttcgcg acggatgacg
     1381 cgttcctggg ttacgtcgtt gccctggatg cggatagcgc caatctggta gcgatctttt
     1441 tcgtcgattt caaaaatcag gttcagcagc gccgggcttc cgccgtctat gtttctggcc
     1501 cattccggtg caggcgaatc ggggctaatg aactgcttgt gtgcgattat gtcggcgtcg
     1561 atatagccta tttcaccgta agcggctttg atggatttta cgtctatctg aagggtttcg
     1621 ccggtgaaga acgattcgcg gtggaacttt atctgttcca ggagctgttc gccgctgtat
     1681 accttgttgc ctttgaaaat gatttcattg acgcggtagc gggggccttc gtttatcttg
     1741 aagacgacgg tgactttgga tttatctgca ctgaactcca gttcacggcc gacttcggcg
     1801 tccaggaaac cttcctggac gtacgcgtta cggatggaaa ttatgtcgcg gtctatcttc
     1861 tccatgttga gatagccctt gatgaagggg aagagcttgg ccttggtttg cgtacgcatg
     1921 atgaggctgg ctttggagaa gtagtgattg ccgtcgaacc gtactttggt gatgatgctc
     1981 tgggggcctt cttctatgga gtatatgacc tggtagccgc cgtctttcat gaccctgttg
     2041 actttgacgt ccgccaggta gtaaccttcg ccgcgatact tgttcagcag cgcgagcttg
     2101 ccctgttcga tatttgccgg gttgagcggg tcgccctggg cgaatgggat atccttgagg
     2161 agttcctctt cggaaaagtg tcggttgcct tctatcatca gtcgggagat ttcgggcctt
     2221 tccgaaacca cgaacgtaag cactacgcct ttgtcggtca cagtgctctt gacgtcggct
     2281 gcgtcgaagc ggccgctctg catcagcctg tcgcggtcgg cgcgaactgc atcttcgctg
     2341 tagggcatgc cttcgcgaat tttgatataa ctcagcacgg cattgcggct ggtggtttta
     2401 ttcccctcga ccttgatttc gacgacggta agatcggggg acttgtctgc aagacccgac
     2461 gaggcaaagc tgaggaaaac cccagccgcc agaaaaattc tcagcagcgt cttcacctta
     2521 tatactccca gcgttggccc ttgttcaaaa agagcgctaa aataaaccga ttccgattaa
     2581 ttccgattcc ttatgggccg tagcccttaa attcgtacca atattgatga tacgtttttt
     2641 agtcggctca ggataacttg cagtcaaacc gctgtcaatg ccctgaacgt cgcagccgac
     2701 ggcgataaaa aataaaaggg gaaaatggaa atttttatct tttttcgcga attttttaac
     2761 gctctgaaaa caaaggcctt aggctgtagc ccaaaaggtc cggaaaaagt tatcaacagc
     2821 tttttacatt catcaaattt tttaagattt ttcggggttt ttcgttctgc atgcgtctgt
     2881 ggcggcttgc aggaattccg tggcgttcga ctcgaattca agcgtatgtt cgccgcggag
     2941 tgttcgaatg ttattttcac tgccgcagac gcgacacagc catcgccggg tagccgcgtt
     3001 gtcgatgatt ctgtcatttg acgaaagtat cgtatcgacc ggtatatcca gcgagccggc
     3061 agacgcttta cgtattgcga ggtcaagcag cctgcttgtg aacatgaatt tcgccgtagc
     3121 cccgtgcagt tgaaattcgt cgtccaacag atatttctgc atcgccgggt tggctgtaaa
     3181 aagttcagcg tcgccaagcg ggatattgaa agttgtttta cgcaagccgg tcatgaggca
     3241 ggcggcgatt gcgagttttt tagccgccgg cacgtctatg agcgaaaaca ggcctggcgc
     3301 tattaagacg agctttgccg ttttttggcg tcgtgccgcg tctgaaagcg cataggcgca
     3361 tattagtttg ccgccccagc ttacgccgag caggattgct tttcccttgc cggtcgtttt
     3421 tagtgcgaaa tcaatcgcgc agtcgatgtc gtcgaaaagc tgccgcggat ttttacaatg
     3481 gccacgcttt attgagttgt cgccgctgcc gcgacgcgtt acctgaaaga ctcgatggcc
     3541 ggcgtcggct aatgtgcgcg cacttcgata aaaccagccc gggtgcgact gaattccgtg
     3601 catgtagact acgggaatat cggctgatac gccggcgggt tcatcgtgga cgtatacgct
     3661 agtgacgtaa ccgtcgccga gcgtcagctc ttcccgatgc ggttgctgcc gggcgtcaag
     3721 ttcgaatgca ggttttggaa aaggtcggtt catgttcata tcatcacggc cctggaggga
     3781 attcaagcgg ccggtatggc gctacgccat aatcgtcgct gtgttcgaac ggtttgcggg
     3841 aaagcattat cggtttgcgg ccggctacgt tgacaagaag gccaatcgcg gcacagttca
     3901 ccaacagcga tgagccgccg taacttatca gcggcagagt catgccggtt atcggcagaa
     3961 gtcccatcgt catggctgta ttgatgacaa tctgcgaaac gaacagcgat attacgccta
     4021 tcgccaacag ccggccgaac ggctcatccg tcgatgccgc tatttccacg ccgagtatta
     4081 taatcaccag atagagcgtt aataccgccg tgcagccgat aaaaccccat tgtccgccta
     4141 ttatgctgaa gatgaaatcc gtgtggtctt ccggcagggt gcggaagtaa aaatctgcgt
     4201 catcccaatc gcctctgccg gaaagctggc cgcagccgag cgttattttt gaacgtgaaa
     4261 gctgatagcc tttgtttttt tctatttccg ggtcgttctg cctcagccat ccgtcgatac
     4321 gttgcagctg gtggaattcc atgacggcta acggcgctgc gctgactatc atgtcgccgg
     4381 acgggctgac ccagtatgcc gttgccttgc gtgagtcgag ttccttttcg cccatgtcct
     4441 gcgtatgagt cggaatcggg aaaaacatca ctataatggt gcagattatt atgccgccca
     4501 ggtgggacat cttcgcgccg gccatgaaca gcatggtgaa cagcacgggt atgagcagta
     4561 tgctcgtgcc caggtcgggt tctatgagaa tcaggaacag cggcacgaac gtgagtatga
     4621 acggtacgct caggccggcc agcttgctgt aatggctgcc cagccgcaga taccacgcca
     4681 gcagcagtat ctctgccagc ttggcaagtt cagacggctg aagttcggca aagcccatgt
     4741 taatccacct gtacgcgccg ttcttggggc gaatcagaaa agaactcgaa tgcgttagcc
     4801 gcgacaggcc cactatgagc agcagcagt
//
LOCUS       DAONFI010000176         4805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_20970_length_4805_cov_3.556632, whole genome shotgun sequence.
ACCESSION   DAONFI010000176 DAONFI010000000
VERSION     DAONFI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20970_length_4805_cov_3.556632"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLK08_06540"
     CDS             complement(<1..1070)
                     /locus_tag="PLK08_06540"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPS53191.1"
                     /translation="MSNPSNAQTTAQPLISKSVNTLSWTWNTPGQLNGWSTARTNSSV
                     VSENGIEVNTGNDFNFVSPTLDVKAEKYRYIALRLKCDLSNAGEIFFTPEGQKHFASK
                     FSVNFQIIGDDEFHTYLVDMTENPKWLGRISRLRFDTENVPEISVQIQKIELLETPDS
                     KETYVRMKPYPVFTRCAGPSSVIRINSEVQFFNALNLDLPGMETLKAAVAAKDWPAAK
                     KALLEYMRKRSQPQYIVNRHEKEKYINKAKEFSRDEIDDAINKADLACEHDFTFEGGR
                     FKFNGPIVWDKTKSGTSFTYNCYLNRMPFLAELGRAYWLTDNSKYVKAACDLINSWIS
                     SCPMPPEIQRLWLYSKNPPSTM"
     gene            complement(1318..2337)
                     /locus_tag="PLK08_06545"
     CDS             complement(1318..2337)
                     /locus_tag="PLK08_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPS53192.1"
                     /translation="MAVTMQQIADRVGVSQVTVSRALNNSSRISKETKDKIYREAKRL
                     NYRPNAIARALAINKTHTIGLVVPDIRNPFTAEITGYIEDSLADSDYSLLIRVTNNSI
                     EKEEAAIFTLLEKRVDGIIINHPQSNINSKCIQQLKRENFPFILIGVIEGLDTDYVMV
                     DLAEGAYLAIKHLLSLGHRRIAFLRSFRTQLRLEGYVKAFHEYGLEVDESLIIKCDPI
                     MDEVIKAVEYVLAMPNRPTAIFGLNDIQVLKAMDVLFDAGLDVPRDMAIVGFDDVMFA
                     SHLRVPLTTVAQPLEQIGKLAVEILLKKIEKQTTKPWQVNLRPTLVVRESCGEKLQNM
                     PLICQ"
     gene            2823..3803
                     /locus_tag="PLK08_06550"
     CDS             2823..3803
                     /locus_tag="PLK08_06550"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPS53193.1"
                     /translation="MAQEFKKICDMLEAAESVLLITHVNPDGDALGSTGALYHSAILA
                     GKKASLMLHDVPSEQYVPLLETVTTHLPDEFAAAAESADVIVIADTGSNVQLGTLAEK
                     LLPFREKTVVIDHHATFDDIAAVRWTDTSAAAAGVMILEIIEILEWPMNETIANLLAR
                     AILTDTGWLRFSNTDGRCLRTMARLVDAGVRLDSLYRSIYQTDRPQKLRLLERTLASL
                     ELYADNQFAVMTLLRTDFEQTGARFDETENLVNEAMRIKTVEVAAIFIENTHNIRASL
                     RSRDFINVAEIAKNFGGGGHARSSGMKVTNMTVDELKKQVVEKILENFQN"
     regulatory      3828..4057
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4250..>4805
                     /locus_tag="PLK08_06555"
     CDS             4250..>4805
                     /locus_tag="PLK08_06555"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HPS53194.1"
                     /translation="MVGFSARERLAIAMKGFFSLTVGVCVLTVTMFAGRLQATGTFDD
                     LTLSANSYWNGESKWQADGSPYPPTPAESPYNYDEGFASCGMEFSNRFTATTYGEESP
                     YLYYTSWSGFAYSNMTDTTTAGYTNQYSVYTGAAYDGSNFGVGFYASWGGMIPSITFA
                     SVSAPQSLMMTNTTYTALSLLNGDS"
BASE COUNT         1270 a         1216 c         1048 g         1271 t
ORIGIN      
        1 cacattgtgc ttggcggatt cttggaatat aaccaaagac gttgtatttc gggcggcatc
       61 ggacatgaac ttatccagct atttatcagg tcacatgctg ccttgacata tttgctattg
      121 tcggtcagcc aatatgcacg gccaagttcg gctaaaaacg gcatacgatt caaatagcag
      181 ttatacgtaa aagacgttcc gctctttgtt ttgtcccata ctatagggcc gttaaacttg
      241 aatctgccgc cttcaaaagt aaaatcatgt tcacaggcaa gatcggcttt atttatagcg
      301 tcatcaatct catcgcgtga aaattctttg gccttattga tgtatttttc tttttcatgt
      361 cgattgacga tatactgcgg ctggctacgt tttcgcatgt attccagcag ggcttttttg
      421 gcggcaggcc aatcctttgc ggcgacggca gccttgagag tctccatgcc aggcaggtca
      481 agattcaggg cattaaaaaa ttgcacctcg ctgttaatgc gaatcacgga agacggccct
      541 gcacatcgcg taaataccgg atacggtttc atgcgcacgt aagtttcttt cgaatccggc
      601 gtctcaagca attcaatctt ttgaatctgg acgcttattt ccggcacatt ttcggtatca
      661 aaacgcagac ggctaatccg gcccaaccat ttcggatttt cagtcatatc gaccagataa
      721 gtgtggaatt catcgtctcc gattatctgg aaattaactg aaaatttcga ggcaaagtgt
      781 ttctggcctt caggcgtaaa aaatatttca ccggcattac tcaggtcaca cttgagccgt
      841 aatgcaatat accgatattt ttcagccttt acatccaacg tcggtgacac aaagttaaaa
      901 tcattaccgg tgtttacttc tataccattt tcggatacta cgctggaatt cgtcctggct
      961 gtactccagc cgtttaattg gccaggcgta ttccaggtcc atgaaagcgt attcacactt
     1021 tttgaaatta aaggctgagc cgtcgtctga gcattcgacg gattcgacat aaagagaatc
     1081 attcctgcaa taaccgcggt aaaatgaccc ataaagcgat tttgttttct catttaactg
     1141 tacctttctt ttctgatttt accgtaaaaa tggcccaaaa ggcatgaaca cacatgttca
     1201 taaacataaa aaaacatttt tctaaagatt ttcttgatac ctacgcagtc acacacctga
     1261 tacttttcaa tagcgatatt ttaaaaacca aatcgaaaag acatctgcta aaaattttca
     1321 ctggcagatt agcggcatat tttgcaactt ttcgccacat gattcccgta ccacaagcgt
     1381 aggccgcaaa ttgacctgcc acggttttgt cgtctgtttt tcaatttttt tcagcaatat
     1441 ttcaaccgcc aatttgccta tctgttccag cggctgagcg acagtggtaa gcggcactct
     1501 aagatgcgat gcaaacatga catcgtcaaa gcccacgata gccatgtcgc gcggcacatc
     1561 caagccggcg tcgaataaga cgtccatcgc cttcaatacc tgaatgtcat tcaagccgaa
     1621 aattgcagta ggtctgttcg gcatagccag aacatactcc acggctttta tcacttcatc
     1681 catgattgga tcgcacttta ttatcagtga ttcatccact tccaagccat attcatgaaa
     1741 cgctttcaca taaccttcca atcgcaactg agtgcgaaaa cttcgcagaa aagctatccg
     1801 cctgtggccc aggctaagca ggtgtttgat ggccagatac gcaccttccg ccaaatcgac
     1861 catcacataa tccgtatcca ggccttctat cacgcctata agtatgaacg ggaaattttc
     1921 gcgtttcagc tgttgaatgc atttactatt gatattgctc tgcgggtggt tgatgatgat
     1981 tccatcgact cgtttttcga gcagtgtgaa aatggcggct tcttcttttt cgatgctgtt
     2041 attggttaca cgaattaaaa ggctgtaatc gctgtcagcc aagctgtctt caatataacc
     2101 agtgatttca gccgtaaacg ggttcctgat gtcgggtacg accaggccaa tggtatgagt
     2161 tttattgata gccaacgccc tggcaatggc attgggcctg taattaagac gcttggcctc
     2221 ccgataaatc ttgtccttgg tctctttact gattcgagaa ctattgttaa gcgcacgtga
     2281 aacggttact tgagaaacac ccacgcggtc agctatttgt tgcatcgtaa cagccatcaa
     2341 actactcctg ctcgactaat aacagcacaa catcttccgc acacagagca agctaacata
     2401 attatcatga aaataaatat tgtaaataat atcatttaat gccgcaatat acggcactgc
     2461 ggttagcgtt ttgttaatga ttattaataa ccaattgcgc aaatatcgcg atattttatg
     2521 caaaacaaca cttatatgcg tcttgtatgc gtgtatacgg ttacatcaca atatgattct
     2581 acactaaaat aatatcagtt caaatagaat ttcccgacaa tccggatttt ttatcaaaaa
     2641 tatctgccgc cgcttcaaac ggcgattctt tacattcttt gcatccacga caaacactac
     2701 gttcatagag caatagtatc agcggcggat attatcttta tgcagccgca attatctact
     2761 agaagacgat ttgcaatttt actacaatgc ctttcgtgtc agaaatatcg tcaggagatt
     2821 cgatggccca ggaattcaaa aaaatctgcg acatgttgga agctgcggaa tccgtcttgc
     2881 ttatcacgca cgtcaatccc gacggagacg cgctcggctc gacgggtgcg ttgtatcact
     2941 cggcaatatt agccggtaaa aaagcttcgt taatgctgca cgacgtcccc tccgagcaat
     3001 acgttccgct tctcgaaacg gtaaccacgc atctgcccga cgaatttgct gctgccgccg
     3061 aatctgccga cgtcatcgtc atcgccgata ctggcagtaa cgttcagctc ggcacacttg
     3121 cggaaaagct actgccgttt cgcgaaaaaa ccgtggtcat agaccatcac gccaccttcg
     3181 acgacatcgc cgccgtacgt tggacggaca catccgccgc agccgccggc gtaatgatac
     3241 tcgaaatcat cgaaatcctc gaatggccga tgaatgaaac catcgcaaat ctgctggccc
     3301 gtgcaatact tacagacacc ggctggttgc ggttttccaa cacagacggc cgctgcctgc
     3361 gaaccatggc ccgccttgtc gacgcaggcg tacggctgga ctcactctac cgcagcattt
     3421 accagactga ccggccacaa aaactccggc tgttggaacg cacactggcc agccttgaac
     3481 tttacgccga caaccagttt gccgtcatga ccctgctacg aacggatttt gagcagaccg
     3541 gcgcaagatt tgacgaaact gaaaatctcg taaacgaggc catgcgcata aaaaccgtgg
     3601 aagtcgccgc aatatttatc gaaaatacgc acaacatccg tgccagcctc cgaagtcggg
     3661 atttcataaa cgtcgctgaa atcgccaaaa atttcggcgg cggcggccat gcacgttcgt
     3721 cgggtatgaa ggtcacgaac atgacagtcg acgaactcaa aaaacaggtc gtggaaaaaa
     3781 ttctcgaaaa tttccaaaat tgatttgcct tttgacgcat ctgatataaa attccgtgca
     3841 ttacaggtgt cctgccggac aggggttgta gcccctaagg gaaggccgtg aaagtcggcc
     3901 gcggacgcgc cgctgtaatc ggcaacgtgt gctgcaaatg ccacttatcc cgtgtgttac
     3961 ggggttggga aggcgcagta gacggtttgc gggatgttgc tctaaccccc gcatgaagcc
     4021 gagagtcaga aaacctgcct gtgataaacc tggttgtgac cttcgcgcca caggcagccg
     4081 ggacgcgata gaatcgtgct cagatagtat tcccgcctcc tgtttgatac ccgcaatggt
     4141 catctgaaac tctttgtgcg cttgcgtctt gaaataaaga caccccgcgc cgtttcctcc
     4201 aggtttgatg ctttgtcgca ttcagtgcgc ttatgcgatg aagcaaaaca tggttggatt
     4261 cagcgcacgg gaaaggttgg ctatcgctat gaagggtttc ttttcactca cggttggggt
     4321 ctgcgttttg acggtaacaa tgtttgcagg ccggcttcag gcgaccggaa cattcgatga
     4381 tttaactctc tcggcaaaca gctactggaa cggcgagtct aaatggcagg ctgacggcag
     4441 cccgtatccc cccacgcctg ccgaatcacc gtataattac gacgagggtt tcgcaagttg
     4501 cgggatggag ttcagcaaca ggttcacggc caccacttac ggcgaagaat cgccatacct
     4561 ttattacaca tcatggagcg gttttgccta ctccaacatg acggacacca cgacggcagg
     4621 ttatacgaac caatacagcg tctacactgg cgccgcatat gacggcagca acttcggcgt
     4681 tggtttttac gcatcatggg gaggcatgat tccttcaatt acgtttgcct ccgtctcagc
     4741 cccgcaaagt ctcatgatga caaataccac ctataccgcc ctgtcgctgc tgaatggcga
     4801 ttcat
//
LOCUS       DAONFI010000177         4791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_21052_length_4791_cov_4.189611, whole genome shotgun sequence.
ACCESSION   DAONFI010000177 DAONFI010000000
VERSION     DAONFI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21052_length_4791_cov_4.189611"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLK08_06560"
     CDS             complement(<1..664)
                     /locus_tag="PLK08_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPS53195.1"
                     /translation="MARKLFGTDGIRGRANTYPVNPEIALKLGKAVGKHFKAIGGFGQ
                     RVVIGKDTRLSGYMLENALTSGLVSMGMDVFLVGPVPTPAVAHLTKSLACIAGFMITA
                     SHNPYTDNGIKIFNADGFKLNDAAEEEIEGYIFDGDLQPSDEDAPKLGQARRIEDARG
                     RYIEFAKSAIRSENIRDMKIVLDCANGATYKVAPIIFTELGAEVITLNDHPNGMNIND
                     HCG"
     gene            1164..2120
                     /locus_tag="PLK08_06565"
     CDS             1164..2120
                     /locus_tag="PLK08_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPS53196.1"
                     /translation="MSIAIDSQPLQEQESQRMMALIAKAAPWTLSALLHVAILLVLLF
                     TVFFTINDIPPAELDIRTEFWAETKPIIAASPMAAAATSMNKHSINPRADKALPVAIK
                     KLPPIIAKKNLNNAQLSLNNPISNSAFDMHISAGSDKCFFDDNAGKTGRADNIVYVID
                     ASGSMMLSGAAQAVRDELYRVLGRLSKKQFFGIIFFTGSRTLTFHKKLLAANSRNIYA
                     AVDFSQGIASTGPSDACDAVRQAFALLKNSPKKRNLICLLSDGELKDSVKVQTLIREL
                     NRGGNVRINTILSGSRESAAMQSMRAIAAGNGGTFKYVDLTD"
     gene            complement(2146..>4791)
                     /locus_tag="PLK08_06570"
     CDS             complement(2146..>4791)
                     /locus_tag="PLK08_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPS53197.1"
                     /translation="ECDAFERVVVKRKAQPRSRVPELDLDNIPEPRKRPTEDVWRCCL
                     TGVGGMGIGLATSILVRAGHNEGYRVIFMDRKGMAIRNGGVFSQVTFNLTQQPVTAVI
                     PHGKADLLIGVDVLEAARILDPKNRVRVISKDKTAAVINMDKVQTIAGIMGREDFDTD
                     ELVRLIQANTRGDEFLARNISRICEKYLGSRLYANVMMLGYAFQQGLIPVSMHSMAWA
                     IKDTIRVDFKKNLYAFNMGRKLVDSPDLFQGPHKRTTWRETLDDKARELQRRFGKNAQ
                     AVAEFRDMLVSLLEKLREFNQTILRDIVIRAYDCVRWGGVDYCRQYVNLVRNICEKDR
                     PEYGYAASGAVVKNLAQAMLIKDAVFKAELSTSHEKYRRDAEKYNVNPANGDRIIYRH
                     LWKRHIKIGSWEKQIRFSVYDWQLKILRRMRFIRSVTRFFMPQEYGFREEYINLTSRF
                     AFGDAEEYRRQVRLLSGPLCQNCYMPSCSQQGCPLGNDVPKWISLAREGKWKVAYAKL
                     AEKNNFPEFTAGICPAFCEQNCHQGIGGYSVQVREMEREIIDRAFEQGWVQPFKPRGA
                     TGKNIAVIGSGPAGLAAAQQLCRTGHTVTVFEKNDAAGGMLRVGVPAARLAKQLIDRR
                     IEQLRQEGVQFMYGVEVGRDVSGESLLHDFDVVCLACGAIVPRELDLPGRAAANIHFA
                     LDYLGSQNRADVEKSQPSISASGKTVVVIGGGLTGEDCVETAMRQGAKEVYQLEILPQ
                     DMTAVLDENPAVKRMWQVRATEFTSQSGSVTGVKALRVEYRPTAKGPVMEKVDGSEFS
                     IAADIVIIAAGFLPQIPSNLAKDLKIQTDNRGSVIIPGKYTTTNSRVFIAGDLATGSA
                     YVATAIDSGRKAARAISSTLRRKTI"
BASE COUNT         1159 a         1291 c         1131 g         1210 t
ORIGIN      
        1 ccccgcaatg gtcgttgatg ttcatgccgt tggggtgatc gttgagcgtg ataacctccg
       61 cgcccagctc ggtaaaaata atcggggcca ccttatacgt ggcgccgttg gcgcaatcga
      121 gcactatttt catatcgcgt atattttccg aacggatggc gcttttggcg aactcgatat
      181 agcggcccct ggcgtcctcg atgcgcctgg cttggccgag tttgggggca tcttcatcgc
      241 tgggctgaag atcgccgtcg aaaatgtagc cctcaatttc ctcttcggcg gcgtcattca
      301 gtttaaatcc gtcggcattg aagattttta tgccgttatc cgtatacgga ttatgcgaag
      361 cggttatcat gaatcctgcg atacacgcca gcgatttagt caggtgtgcc actgccggcg
      421 tcggcacagg gccgaccagg aaaacatcca tgcccatcga aaccaggccg ctcgtaaggg
      481 cattttccaa catgtacccg ctcagccgcg tatccttgcc gataaccaca cgctgcccga
      541 atccgccgat agccttaaaa tgtttgccaa cagccttgcc gagcttgagc gcaatttcgg
      601 ggttcaccgg ataggtgtta gcccgcccgc gtatgccgtc cgtaccgaat aattttctcg
      661 ccatttgtcc tgccttccaa aatccttttc cccatacttc atattaaagt ataggtctca
      721 tacgataaat taatgataac aaagaaattg ttaaatacaa taatttttaa catctttaga
      781 ataaacatgt tatgttttat atgcagcgtt ttagttaaaa acttacccca aacatccgtc
      841 agtcgcaact tgcacggctg ccggaaacca agttttagcc ttattttaaa ggattctcag
      901 caataaactt aattccaaaa aaggtctcct tttaaatctt acacgttttc agccgccagc
      961 ctaatcagcg taatagttaa aaataattta cataactcct tattatacaa ggtccgatat
     1021 tttttcatgg tatttttgtt aacaggctta ttcaaacaaa aaaaccgcag gcaaccacgt
     1081 tcagggataa ttcaaaatcg gggaacattt tcagcccgat atcatccaag tatttagatg
     1141 tgtattttaa ggagacagcg aaaatgtcaa tcgcaatcga cagccagccg cttcaggaac
     1201 aggaaagtca acgaatgatg gcacttatag caaaggcagc gccctggact ctcagtgcgt
     1261 tactgcatgt cgccattttg ctggtgctgc tgttcacggt atttttcaca attaacgata
     1321 tcccgccggc ggaattagat atacggacgg aattctgggc tgaaacaaaa cccattatag
     1381 ctgcctcgcc aatggccgcc gctgccacgt ctatgaataa acattccata aacccgaggg
     1441 cagataaagc tcttccggtc gccattaaaa aactgccgcc aattatcgcc aaaaaaaact
     1501 taaacaatgc tcagctttct ttgaataatc cgatatcgaa ttcggcattt gacatgcata
     1561 tttcagccgg aagcgataaa tgcttttttg atgataatgc cggcaaaaca ggccgcgcgg
     1621 ataacatcgt atacgtcatc gatgcctccg gttcgatgat gctttccggc gcagcgcaag
     1681 ccgttcggga tgaactttac agggtattgg gccgattgtc gaaaaaacag tttttcggaa
     1741 taatcttttt caccggcagc cggacgttga cgtttcacaa aaaactgctt gcggccaatt
     1801 cccgaaatat ttatgccgcg gtggattttt ctcaaggcat tgcaagcacc ggcccgagcg
     1861 atgcctgcga cgcagtacgt caggcatttg cattattgaa aaattcgccg aaaaaacgca
     1921 acctgatatg cctgctcagt gacggcgaac tgaaagattc cgtgaaagta caaacattaa
     1981 tcagagaact caatcgcggc ggcaatgtac gaatcaatac cattttgagc ggcagcaggg
     2041 aatcagcggc tatgcaatcc atgcgtgcaa tcgcagccgg caacggcgga acgtttaaat
     2101 acgtcgacct gacagactaa agtttttata atctggaaaa tgtaactata tggttttacg
     2161 tcgcagagtc gatgatatgg cacgggcggc tttgcggcct gagtcgatag ccgttgcaac
     2221 ataggccgag ccggtcgcca aatcacctgc tatgaacact cgcgaattgg tggtcgtata
     2281 tttgccagga ataatcacac tgccgcggtt gtccgtctgt attttcaaat ctttcgcaag
     2341 atttgacgga atctgcggca gaaaacctgc tgcgattatg acgatatcgg cggcgattga
     2401 aaactcactg ccgtcgactt tctccataac cgggcctttc gccgtcggcc tatattccac
     2461 ccgcagagcc ttaacgcccg taacactgcc gctttgcgac gtaaattcag tcgccctgac
     2521 ctgccacatg cgtttgacgg ctggattttc atccagcacc gccgtcatgt cctgcggcag
     2581 aatttcaagc tgataaactt ccttcgcgcc ctgccgcatc gccgtttcca cgcaatcttc
     2641 gccggtaagg ccgccgccta tgacaaccac cgtcttgcct gaggcggaaa tagacggctg
     2701 tgatttttcg acatccgcgc gattctggct gccgagatag tccagcgcga aatgaatatt
     2761 cgccgccgct ctgccgggca gatcaagttc gcgcggcact atagcaccgc aggccagaca
     2821 gacaacatcg aaatcatgca acagcgattc gcctgaaaca tccctgccga cttccacgcc
     2881 gtacatgaat tgcacgcctt cctggcggag ttgttcgatg cgtcgatcta taagctgctt
     2941 ggccaatctt gcagccggca cgccgacccg caacatgccg cctgccgcat cgttcttttc
     3001 aaacacagtc actgtatggc ctgtgcggca gagctgctgc gcagcagcca ggccggccgg
     3061 cccgctacct atcaccgcaa tatttttgcc cgtcgctcca cgcggtttga acggctggac
     3121 ccagccctgt tcgaaggcgc ggtcgattat ctcccgctcc atttcccgaa cctgcacgga
     3181 atatccgccg ataccctgat ggcagttctg ctcgcagaac gcaggacaga tgcctgcggt
     3241 aaattccgga aaattgttct tttccgcaag tttagcgtag gcgactttcc atttgccttc
     3301 gcgagccagg ctaatccatt tcggcacatc gttgccgagc ggacagccct gctgggaaca
     3361 cgatggcata tagcaattct ggcacaaagg cccggaaagc agacgcacct gacgacgata
     3421 ttcttcggca tcgccgaatg cgaatcgact ggtcagattg atgtattctt ctcggaagcc
     3481 gtattcttgc ggcatgaaaa atcttgtaac ggaacgaatg aaccgcatcc gtcgcaagat
     3541 tttcagctgc caatcatata cgctgaaacg aatctgtttt tcccatgagc cgatttttat
     3601 atgacgcttc cagagatgac ggtaaataat tctgtcgccg ttagccggat tgacgttgta
     3661 tttttcggca tcgcgacgat atttttcatg gctggtggaa agttcggctt tgaatacggc
     3721 atcctttatg agcatcgcct gcgccagatt tttcactaca gcgccggatg cggcataacc
     3781 gtattccggc cggtcttttt cgcaaatgtt ccgaacgaga tttacgtact gtctacaata
     3841 atccacgccg ccccaccgga cgcaatcata agcgcgaatt acgatatccc gcaaaatggt
     3901 ttggttgaat tcgcgaagtt tttccagcaa cgaaaccagc atgtcgcgaa actcggcaac
     3961 cgcctgagcg tttttgccga atcgacgctg aagttcgcgg gctttatcgt ccaacgtctc
     4021 acgccatgtc gtgcgtttgt gcgggccctg gaacaggtcc ggactgtcaa cgagttttcg
     4081 gcccatattg aatgcgtaaa gattcttttt gaaatcgacg cgtatcgtat cctttattgc
     4141 ccaggccatc gaatgcatgg aaaccggaat cagtccctgc tggaaagcgt aaccgagcat
     4201 catcacgttg gcatacagcc tgcttcctag atatttttcg cagattcgtg aaatattgcg
     4261 agcgagaaat tcatcgccgc gcgtattagc ctgtatcagc cgcacgagtt catccgtgtc
     4321 gaaatcctcg cgtcccataa tgccggcgat ggtttgcact ttgtccatat tgatgacagc
     4381 cgccgtttta tctttgctta ttacacggac gcggtttttc gggtcgagga ttctggcggc
     4441 ttcaaggacg tccacgccga tgagcaggtc ggctttgccg tgcggtatta cagcggtgac
     4501 cggctgttgc gtgaggttga acgtcacctg agaaaacact ccgccgttgc ggatagccat
     4561 gcccttgcga tccatgaata tgacgcgata gccttcatta tggccggctc gcacaagtat
     4621 gctggtcgcc aggcctatgc ccatgccgcc cacacccgtc aggcagcatc gccagacatc
     4681 ttccgtcgga cgcttgcgag gttcgggaat attgtccagg tcgagttcgg gcactctgct
     4741 tctgggctga gccttgcgct tgactacaac tcgctcgaac gcatcgcact c
//
LOCUS       DAONFI010000178         4789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_21071_length_4789_cov_3.609210, whole genome shotgun sequence.
ACCESSION   DAONFI010000178 DAONFI010000000
VERSION     DAONFI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21071_length_4789_cov_3.609210"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1390)
                     /locus_tag="PLK08_06575"
     CDS             complement(53..1390)
                     /locus_tag="PLK08_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53198.1"
                     /translation="MSEWMDADSRVERAHDLYCKGRWVEAAAELRAAISSNPYNPSWH
                     FNLGLTLEALDDFKGAIRAFSENVRLDPNDIEGLCRLGVNLGRLGKFDLALKTFQRVQ
                     KLDPNYEPGYCNRIIIYTEMGQHEDAEVMFYMAQQLNDACPLCYFNIGNSLYARKRYE
                     AAIDCWMKCLELDPTQPQAHSRIGDAYWAMGRLDLALNEFQMELDICESDDIDALLDM
                     GELLTEMNRTDEAEHYFLTVLKKEPENADAHFFLGEIAEKRGDADAAAYRYRKTLKKD
                     SKFKGANLRLGRLAMHTDQRRTALTHLKAELDLNDDNPQLLQEIGELLIEGKMVELAY
                     HAFARLAALLPNDANALHNLAVCCFMLNRMEEGIVHCRKSIRICGWYILAIYNLALAY
                     KCLGRNKRALALTIKAFRLSPKDPNVIMLRKRMGFGGWFGYLKLALIAAKHGR"
     gene            complement(1642..2193)
                     /locus_tag="PLK08_06580"
     CDS             complement(1642..2193)
                     /locus_tag="PLK08_06580"
                     /inference="COORDINATES: protein motif:HMM:NF022389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HPS53199.1"
                     /translation="MTNLAQKQFLKSLRLNLFNRSLHPELFEIYDSLTVDNGGFEAVI
                     WLTRCSHVVSFYTATSALSEVIADDSVPLPCRKKLLSVPMRGEKTHEFQNIDDIRYMT
                     GFQVETMTPRTYQAAHREIKRRADRNGMFVEFPADSSNDLPAFTYINHEILPKGLHVM
                     SFHALSGISTFVKTQSIFEIIEK"
     gene            complement(2102..2773)
                     /locus_tag="PLK08_06585"
     CDS             complement(2102..2773)
                     /locus_tag="PLK08_06585"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPS53200.1"
                     /translation="MPIKHSLKYVTKSVEAVLKTGEQFLVHRVLSLDDTPHRIALGVA
                     IGIFVTWTPTIGIQMALTVALCFLFRANKLVGVPFVWISNPVTIVPVYYPNYVVGCWM
                     LGKKADAWNLIEQAFQFNGGWWDTTVNWFTSIMKIFWELWLGSIIVGGALGIISYFVI
                     YWLVVQYRKGILRWHKKHDHPGTQEEVIQAVIVNDESGTKAVSQEPSAESVQQVTPSG
                     TVRDI"
     gene            complement(3032..4069)
                     /locus_tag="PLK08_06590"
     CDS             complement(3032..4069)
                     /locus_tag="PLK08_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPS53201.1"
                     /translation="MANQSENISAPQKALIIKPSSLGDVITALPVLRGLKRTHPATKV
                     DWLISTACMDILRDDGQINALVPFERKRLGRCWRSADAAGALWQFYRTLAGGEYDWVI
                     DLQGLVRSGIFSLFTHAKLRAGFGDAREGGHIFYNHAFTPQLPHTVDRNIELARSLNL
                     DAKPQDMYLKIDDEAKKTIQNMLPGGDYIVCVPPTRWNTKKYPVRHWRKVIAAISARI
                     TVVISGSPAADEMTLCQSVAENMGPQVINMAGKTSLREMAALIAGSRGVICSDSAAKF
                     IAPAVGVDCVTLLGPTRTEMTGPYLKGKALVADVPCSGCLKKRCNHITCMQSIQPELV
                     ISAAMDMIDRH"
     gene            complement(4053..>4789)
                     /locus_tag="PLK08_06595"
     CDS             complement(4053..>4789)
                     /locus_tag="PLK08_06595"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPS53202.1"
                     /translation="KQVDVHIKQARRKKKINAILDCLRPSRAVSAFRLGQMFLTRRIP
                     TPLPMAALEKRIGPFLLDSILITETLDSPSLQRFMTCNLTTQPAKSTKRPEWEKCIYR
                     TMEILGKSLQYMHDMGFTHRDLKATNMIVFIDSDQIPEIAFLDLDAVSKEFRVTLRQR
                     YKGLTRVFVALQQCHTLTHAGFMRLLMAYMRRPGCGELYYKPYWRLIERWGENKLTKQ
                     IKSRRKQQKAQRKWFFTLNHKNGQSI"
BASE COUNT         1078 a         1208 c         1207 g         1296 t
ORIGIN      
        1 ccacgaacaa acccactggc gcaaaaatat gaactctcaa tctttcaatg ctctatctgc
       61 cgtgtttggc ggcgattaac gccagtttca gatagccgaa ccagccgccg aatcccatgc
      121 gttttctgag catgattaca ttggggtctt taggcgacag ccggaaggct tttatcgtca
      181 gggctaatgc ccgtttattc cggcccaggc atttgtaggc taacgcgagg ttgtatatcg
      241 ccaatatgta ccagccgcaa atccgtatcg acttccggca gtgaactatg ccttcctcca
      301 tgcggttgag catgaaacag cagacagcca gattgtgcag cgcgtttgca tcattcggca
      361 gcagggcagc cagccgcgca aaagcgtgat acgcaagctc aaccatcttg ccttcgataa
      421 gcagttctcc gatttcctga agcagctgcg gattgtcgtc gttcaggtca agttcagcct
      481 tgaggtgagt gagggctgta cgtcgctggt cagtatgcat ggccaggcgg cccagtcgca
      541 ggttcgcacc cttgaatttg gaatcttttt ttagggtctt gcggtaacga taggcggctg
      601 cgtcagcatc gccacgcttt tcggcgattt cgccgaggaa gaaatgcgcg tcggcgtttt
      661 ccggctcttt tttcagaacc gtcagaaaat aatgttcagc ctcatctgtg cggttcattt
      721 ccgtcagcag ttcgcccatg tcaagcaggg cgtcaatgtc gtcagattcg cagatatcca
      781 actccatctg gaattcattt aacgccaggt cgagtctgcc catcgcccag tatgcatcgc
      841 ctatgcggga gtgagcctgc ggctgagtgg ggtcaagctc aagacacttc atccagcagt
      901 ctatcgctgc ctcgtaacgc ttgcgggcgt aaagactgtt gccgatattg aaataacaca
      961 gcggacaggc gtcgttgagc tgctgagcca tgtagaacat cacctccgca tcctcgtgct
     1021 gtcccatttc ggtgtagatt atgatgcggt tgcaatatcc aggctcatag ttcgggtcaa
     1081 gtttctggac tctctgaaac gtcttgagag ccaggtcgaa tttgcccagc cggccgaggt
     1141 tcacgcccag ccgacaaagt ccttctatat cgttcgggtc cagccggacg ttttctgaaa
     1201 atgcccttat tgcgcccttg aagtcgtcca gcgcttcgag cgtcagtccc agattgaagt
     1261 gccatgacgg attgtaggga ttagaactta tagcagcccg cagttctgcg gcagcttcga
     1321 cccagcggcc tttgcagtaa aggtcgtgcg cacgttccac tctggagtcg gcgtccatcc
     1381 actcactcat ggttccacca ttcccgacgc acaaatagcg ccagtacagc tttgtcagcg
     1441 tgttttttta taacacaggg gctgtcagca acgacatccc acgatatgtt gttttgcgtc
     1501 ttcaatatat tgtatacccg ataaaagaaa ttcaaagcaa tttcgaaatt cttttataca
     1561 gctgaagttc tcaaaagcct ttccgagcgg cataatttcc cataaaatgt gaaaaatgca
     1621 acaaaatttc gggaatcggc attatttttc gataatttca aaaattgact gtgttttcac
     1681 gaaagtggaa attccgctaa gtgcgtgaaa agacatgaca tgcaggcctt tgggcaatat
     1741 ttcatgattg atgtaggtaa acgccggcaa atcatttgac gagtcagccg gaaattctac
     1801 gaacatgccg ttcctgtcgg ctcgccgctt tatttcgcga tgggcagcct ggtaagtgcg
     1861 cggcgtcatg gtttcaacct gaaatcctgt catgtaacgg atatcgtcaa tattctgaaa
     1921 ttcatgcgtt ttttcgccgc gcattggcac gctgaggagc tttttgcggc acggcagtgg
     1981 aacggaatcg tcggcgatga cttcgctcag ggctgacgtg gcggtgtaga agctcaccac
     2041 gtgcgaacat ctcgtcagcc agatgacggc ttcaaagccg ccgttatcaa ccgtcagcga
     2101 gtcatatatc tcgaacagtt ccggatggag tgacctgttg aacagattca gccgaaggct
     2161 cttgagaaac tgcttttgtg ccagattcgt catttacgat tacagcctgt attacttctt
     2221 cctgtgtacc tggatgatca tgcttcttgt gccaccgcag tatgcctttg cgatactgca
     2281 ctaccagcca gtatatcaca aagtaggata taattccaag cgctccgccg acaattatgc
     2341 tccccagcca caattcccag aaaatcttca ttatggaagt gaaccagttg acggtcgtgt
     2401 cccaccagcc gccattgaac tgaaatgcct gctcgattaa attccatgcg tcggcttttt
     2461 tgccgagcat ccagcatcct acgacatagt tgggataata gaccggcacg attgttacgg
     2521 gattggatat ccagacgaac ggcacgccca ccaacttgtt ggctcggaac agaaaacata
     2581 acgccacagt caacgccatc tggattccta tggtaggcgt ccatgtcacg aatatgccta
     2641 tcgccacgcc caaggctatc cggtggggag tatcgtccag actgagaacg cggtggacaa
     2701 ggaactgctc gcctgtcttg agcacagcct ccacgctttt agtgacatat tttaatgaat
     2761 gcttaatcgg cattttttta aatgacagtt aatccactta cgtttacttt ggtatttttt
     2821 taaaatgaca atcaatccac ttacgtttat tttggcattt tttttaaatg acaatcaatc
     2881 cacttacgtt tattttggca tttttttaaa tgacagttaa tccacttacg attactttgg
     2941 cattttttta aaatggcaag caatcaactt acgattactt tggcattttt ttaaaatgac
     3001 aatcaatcca cttacattta tttaaaatta attaatgtct atcaatcata tccattgccg
     3061 ctgaaattac aagttccggc tgtatggatt gcatgcatgt tatatggttg cagcgttttt
     3121 tgaggcatcc gctgcaaggg acgtcagcga cgagtgcttt gcctttcagg tatggccctg
     3181 tcatttccgt tcgcgtcggc ccaagcagag tgacgcagtc gactcccacc gccggtgcga
     3241 tgaattttgc tgccgaatcg ctacatatta cgccccgcga gccggcgatt aatgccgcca
     3301 tttcccgcag ggatgttttg ccggccatat ttatgacttg cggccccata ttttccgcga
     3361 cgctttggca gagtgtcatt tcatcggctg ccggcgaacc gcttatgacg acggttattc
     3421 tggctgatat tgctgcgatt acttttcgcc agtgtcgcac gggatatttt ttggtattcc
     3481 accgggtcgg cggcacgcat acgatatagt cgccgccggg cagcatgttt tgaatcgttt
     3541 tttttgcctc gtcgtctatt ttgagataca tgtcctgtgg cttggcgtcc aggttaagcg
     3601 agcgggcgag ttcgatattg cggtcgacgg tatgcggcag ttgcggcgta aaggcgtgat
     3661 tgtagaagat atggccgcct tcgcgggcgt cgccgaagcc ggctcgcagt ttcgcgtgcg
     3721 taaagagcga aaaaataccg ctacgcacca ggccctgcag gtctatcacc cagtcatatt
     3781 caccgcctgc gagggtacgg taaaattgcc acaatgcacc ggctgcgtcg gctgaccgcc
     3841 agcagcggcc gagacgttta cgctcgaacg gcacaagtgc gtttatctgg ccgtcgtcgc
     3901 ggaggatgtc catgcaggcg gtcgatatca gccagtcaac ctttgtcgct ggatgggtac
     3961 gtttaaggcc gcggagtacc ggcagggcgg ttatcacgtc gccgagcgaa cttggcttga
     4021 tgattaatgc tttttgcggg gcggaaatat tttcagattg attggccatt tttatgattt
     4081 agggtaaaga accattttct ttgggctttt tgctgtttcc ggcggctttt aatctgtttt
     4141 gtgagtttat tttcgcccca tcgctctatc agccgccagt aaggtttgta ataaagctcg
     4201 ccgcagcccg gccgacgcat gtaagccatc aacagccgca tgaaaccggc atgcgtcagt
     4261 gtatgacact gctgcaatgc cacgaataca cgcgtaaggc ccttgtaacg ctggcggagg
     4321 gtgaccctga attccttgct gaccgcgtcc aggtcgagaa aggcgatttc aggaatctgg
     4381 tcgctatcga tgaacactat catgttggtg gctttgaggt cgcgatgcgt aaagcccatg
     4441 tcatgcatgt actgcaacga cttgccgagt atttccatag ttcgatagat gcatttttcc
     4501 cattcaggcc gttttgtcga ttttgccggc tgggttgtga gattgcatgt catgaatcgc
     4561 tggaggctcg gcgagtccag cgtttcggta attaaaatgc tgtcgagcag aaaaggcccg
     4621 atacgtttct ccagggcagc catcggcagg ggcgtcggta tgcgtcgcgt caggaacatc
     4681 tggccgagcc ggaaagcgct gaccgcgcgg gacggccgca gacagtctag gatggcgttg
     4741 attttcttct tgcgcctggc ctgttttatg tgcacgtcga cctgcttgt
//
LOCUS       DAONFI010000179         4722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_21515_length_4722_cov_4.393400, whole genome shotgun sequence.
ACCESSION   DAONFI010000179 DAONFI010000000
VERSION     DAONFI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21515_length_4722_cov_4.393400"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLK08_06600"
     CDS             <1..224
                     /locus_tag="PLK08_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53203.1"
                     /translation="PLTYGLGNRRSIHLSYERKFLRNIDSCRLDGQSSAQQLYQPIAY
                     CKTYLQSRKSLALITLVVKCCIEVSRQNL"
     gene            236..1342
                     /locus_tag="PLK08_06605"
     CDS             236..1342
                     /locus_tag="PLK08_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53204.1"
                     /translation="MPHKKINPLEIEVLPLSQRKNYLDIERLTAKIAADTPIPSPAMA
                     AQVDALASRIKAARLADKSVMLAYGAHLIKNGGGVLLRALMDGGYVTHLATQGAGTIH
                     DWEFAWQGKSSESVADNAPQGRFGSWDETGRWINLAVIAGAAEGLGYGESLGRLISTE
                     KLALPTTARLEEMICENPADELTAARADLLATMRRFNLPAGEIAVPHLFKKFSALGGA
                     YERNIPFTVHPGIGYDIIVNHPFYCGAAIGRGAETDARIFADSVLNLGVYMSVGCAIM
                     SPQVFEKAFSLANNLRRTRGHELLRDHYIAVVDIQNGGGWNWKNGEPPKDNPAYYLRF
                     CKSFYRMGGVTDYLCGDNIVVLKALLERLKTNVD"
     gene            1537..2214
                     /locus_tag="PLK08_06610"
     CDS             1537..2214
                     /locus_tag="PLK08_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HPS53205.1"
                     /translation="MKRSFINKKIDEAIAFCTANKFHLPQWAYWTAEKWASVGKEADE
                     IRRCNLGWDVTDFGSGDYANVGLLAFTIRNGEISKSGPTAKNYCEKLLLTGEGQYTPT
                     HFHWSKMEDIINRGGGKLEIQFWNANPKTEEIDTVSDVTVSVDGIVRTIPAGGKILLS
                     PGESVTIQPYLYHNFCAATGTGMVLGGEVSRVNDDSNDNRFNPKLPRFSAIEEDQPKK
                     YLLCNEY"
     gene            2458..3522
                     /gene="pilM"
                     /locus_tag="PLK08_06615"
     CDS             2458..3522
                     /gene="pilM"
                     /locus_tag="PLK08_06615"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPS53206.1"
                     /translation="MAEKKQKNVLPIGIDIGTDMIKIAQLVRNGDGFMLQACAEAVVP
                     AHVRNDPLARLEFFSSAIPKVIREGSFSGKKCVLSVPAVNTLVRHVKVPRLDPAATRT
                     AILRTAQDELSYPVADAVVQYLPITEIYTEGEPRQEILLIAVPRTTVEAYMTMAGHAH
                     MDVLGLSIEALAIADCFVTLFDWAKDESQSNLFLDIGHATAQVVVTHGPKVAFVRNLE
                     KGMTLLDESISDAMKISVEEARSARTRATGLDASGDEYMKIKPVLEPWLEGLADAIDK
                     SLLYYQALYRKSGVNRIVFTGGFAQERRLCQLLAQRLNLPAQVGDPVSGLAGGMERLM
                     GMKTPASSMAVCFGLSLGNF"
     gene            3785..4537
                     /locus_tag="PLK08_06620"
     CDS             3785..4537
                     /locus_tag="PLK08_06620"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPS53207.1"
                     /translation="MNQINLLPEEFIKERFRRRIDLTLVILFAIVMVSVIMAERLSSR
                     KFDAVTQTQTREDGQFAQVDNSSKDFFALLTKRSAYIGDVQKAKALEQKVPRSYLLAV
                     INNACGDELSLEKINCNIFEQVQVQRTGKRVVITSSTANPTKPKEMDIIDIYGVAASD
                     GDVTDFVSRLKSNPVFSEVNLIQTKEWTPMKKPSDQQQKLFEPNRDFSVTLKINPNVD
                     VEELLKKPAAAKPQPPKSAPKSDATKNKGDAK"
     gene            4534..>4722
                     /locus_tag="PLK08_06625"
     CDS             4534..>4722
                     /locus_tag="PLK08_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53208.1"
                     /translation="MKITIREIIFFALLFCLPLATYLLVFKPRQVAERTLNEDISTKQ
                     QIRMEFDRYRIMATENLEE"
BASE COUNT         1305 a         1116 c         1231 g         1070 t
ORIGIN      
        1 aacctctgac ctacggttta ggaaaccgtc gctctatcca cctgagctac gagcgcaaat
       61 ttttgagaaa tatcgactct tgccggctag acgggcaaag ttccgcacaa cagctttacc
      121 aacctattgc ctattgtaaa acttatctgc aaagccgcaa aagtctagca ttaataacat
      181 tagtggtcaa gtgttgtatt gaagtcagcc gccaaaacct ttagactata aaaatatgcc
      241 gcataaaaaa ataaatccgc ttgaaataga agttctaccg ttaagccagc gaaaaaacta
      301 tctcgatata gaacggctga cggcaaagat agccgccgat acgcccatac cgtcgccggc
      361 tatggcagcg caggtggacg ctcttgccag ccgcataaaa gccgccagac ttgcagataa
      421 aagcgtgatg ctggcctatg gtgcgcatct tatcaaaaat ggcggcggcg tattgctgcg
      481 ggcactcatg gacggcggat acgtcacgca tctcgcgaca cagggggcag gcacaataca
      541 cgattgggaa ttcgcgtggc agggcaaatc gtcggaatcg gtggctgaca atgccccgca
      601 gggccgattc ggcagctggg acgaaaccgg cagatggata aatcttgccg tcattgccgg
      661 cgccgcggaa ggcctcggtt acggcgaatc gttgggcagg ctgatatcga cggaaaaact
      721 cgcattgccc actactgcac ggctggagga aatgatttgc gaaaatccgg ctgacgagct
      781 taccgccgca cgcgcggatt tgcttgccac aatgcgacgt ttcaacctgc cggctggcga
      841 aattgcagtt ccgcaccttt ttaaaaaatt ttccgcgctg ggcggcgcct atgaacgcaa
      901 cattccgttc acggttcatc caggcatagg gtacgatata atagtgaatc atccttttta
      961 ctgcggcgcg gcaatcggcc ggggagcgga aaccgatgca cgaatttttg ccgacagcgt
     1021 gttgaatctc ggagtatata tgtcggtcgg ctgtgctata atgtcgccgc aggtttttga
     1081 aaaggcgttc agcctggcga acaatctgcg acgaacccgc ggccatgaat tgctgcgaga
     1141 ccattacatt gcagtggtgg atattcagaa cggcggcgga tggaactgga aaaacggcga
     1201 accgccgaaa gataatccgg cgtattattt gcgattctgc aaatctttct atcgcatggg
     1261 cggtgtgacg gattatctgt gcggcgataa tattgttgtg ttgaaggctt tgcttgagcg
     1321 gctgaaaaca aatgtcgact gatatgcgtt tacggctgat atgcagataa acgccaaagc
     1381 ggtcgcttcg cttgacaaat taatttttta tagtctttac cttttagcat cacaggcgaa
     1441 aatcggttag attacggttt gaaatatatc aaaaattaaa aaagttaaaa gaagttaaaa
     1501 aatccctgcg ttataaattt tttcatggag actccgatga aacgcagctt tataaacaaa
     1561 aagattgacg aagctatcgc attctgcacc gccaacaaat tccatctgcc gcaatgggca
     1621 tactggacgg ctgaaaagtg ggccagcgtc ggaaaggaag ccgatgaaat acgtcgttgc
     1681 aacctgggtt gggatgtgac agatttcggc agcggcgatt atgccaatgt cggcttgctg
     1741 gcgtttacga tacgcaacgg agaaataagc aaaagcggac cgacagcgaa aaattactgc
     1801 gaaaaactgc tgctcaccgg cgaaggccaa tatacgccga cccattttca ctggtcgaag
     1861 atggaagaca tcatcaatcg cggcggcggc aaactggaga tacaattctg gaacgccaac
     1921 ccgaaaaccg aagaaatcga cacggtttcc gacgtgacgg tcagcgttga cggcatcgtc
     1981 agaacaatac cggctggcgg gaaaatactc ctgtcgcctg gagaatccgt gaccattcag
     2041 ccgtatctct atcacaattt ctgtgcagcg acaggcacag gcatggtact gggcggcgaa
     2101 gtctcgcgcg taaatgacga ctcgaacgat aatcgcttta atcccaagct gccgcggttt
     2161 tcggctatcg aagaagacca gcctaaaaaa tacctgctgt gcaacgagta ttaattaacc
     2221 cgttttatac acatatcttc cattgccatg aacgttggac atgtgttttg gagtcgttcc
     2281 ggaaggtcgg ttttgttgac ttagtggtgt taatagccga attttgtgaa attcatgcgg
     2341 tttttgatat aaattgctgc cgaaactgaa aatgcggttt gcaaaaagcc ggctgcggct
     2401 tatataatta cgcataatga aataattgga agcgaggcaa actgccggaa aaattatatg
     2461 gcggaaaaaa aacagaaaaa cgtattgccg ataggcatag atatcggcac ggatatgata
     2521 aaaatcgccc aactggtcag aaacggcgat ggtttcatgc tgcaagcctg tgccgaagcc
     2581 gtcgtgcccg cacatgttcg caatgaccct ctggcgcggc tggagttttt ttcatccgct
     2641 atcccgaagg tgataaggga gggaagtttt tcgggcaaaa aatgcgttct gtccgtgccg
     2701 gctgtcaata cgcttgtacg acacgtcaaa gtgccgaggc ttgacccggc agccaccaga
     2761 acagccatcc tgagaaccgc ccaggatgaa ctctcctatc ccgttgccga cgcggtggtt
     2821 caatatctgc cgataaccga aatatacacc gagggcgagc cgcggcagga aattcttctg
     2881 atagctgtgc cgcgaacaac ggttgaagca tacatgacca tggctggcca cgcgcacatg
     2941 gacgtgctgg gcctcagcat agaagcgttg gccatcgccg attgtttcgt gacgctgttt
     3001 gactgggcga aggacgagtc gcagtcaaat ctctttctcg acatcggcca cgctacggcg
     3061 caggtggttg tgacgcacgg gccgaaggta gcgtttgtgc ggaatcttga aaaaggcatg
     3121 acgctgctcg acgagagcat atccgacgcc atgaaaattt ccgtcgagga ggctcgctcg
     3181 gcaagaacgc gtgccaccgg actggatgcc tccggcgatg aatatatgaa aatcaaacct
     3241 gtcctggaac cctggctgga ggggctggcg gatgcgatag acaaaagcct gttatactat
     3301 caggcactgt atcgaaaatc cggcgtaaac aggatagttt ttacgggcgg cttcgcccag
     3361 gaaagacggt tatgtcaatt attggcccag cggctgaatc tgccggcgca ggtaggcgat
     3421 ccggtatcgg gtttggctgg cggcatggaa aggctgatgg gcatgaaaac gccggcaagc
     3481 tccatggcag tatgcttcgg cctttcactt ggtaattttt agagcatgct ctgatatgtt
     3541 gttcgtaatt ctacggtttt tagaaaagtt gcctgataaa agaatatttt tttgagaacc
     3601 gcggtaagcc atagatgaaa gtatccgccg tcagacggat atgcgaaagg tttgataaat
     3661 atgatgctac gggttttagc ccgtaaagat ttgtttttta tgctgattat ggctgtttgt
     3721 agcgttgggc tggccataaa taaaatagcc cgatttgaag acattaatca ctggcggtac
     3781 cggaatgaac cagataaacc tgttacccga agagttcatc aaagaacgtt tcaggcgaag
     3841 aatcgacctg acgctggtta tcctgtttgc aatagtgatg gtaagcgtca tcatggctga
     3901 acgcctgagc agcagaaaat tcgacgccgt cacgcagacg cagacgagag aagacggcca
     3961 atttgcacag gttgacaatt cgtcaaaaga tttctttgcc ctgctgacga aacgcagcgc
     4021 ttatatcggc gacgtccaga aggcaaaggc gcttgagcag aaagtgcctc gcagttatct
     4081 tctcgccgtc atcaacaacg catgcggcga cgaactttcg ctggaaaaaa tcaactgcaa
     4141 catcttcgaa caggtgcagg tgcagcgaac cggaaaaaga gtcgtcataa cttcttcgac
     4201 ggctaatccg acaaaaccga aggaaatgga tataatagac atttatggcg tggcggcttc
     4261 tgacggcgac gttactgact ttgtttcgcg gctgaagagc aatccggttt tttccgaagt
     4321 caacctgata cagaccaagg aatggacgcc gatgaaaaaa ccgtccgacc agcaacagaa
     4381 actttttgag cccaataggg atttcagcgt aaccctcaaa atcaatccaa acgtggatgt
     4441 ggaagaactc ctcaaaaagc cggcagccgc aaagcctcag ccgcccaaat ccgcccccaa
     4501 aagtgacgca actaaaaaca agggggacgc gaaatgaaaa taaccataag agaaattata
     4561 tttttcgcat tgctcttctg cctgccgctt gctacatatc tgctggtatt caagccccgc
     4621 caggtcgccg aacggactct gaatgaagac atatccacca agcagcagat tcgcatggaa
     4681 tttgaccggt atcgcattat ggcgacggag aacctcgaag aa
//
LOCUS       DAONFI010000180         4662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_21912_length_4662_cov_3.834817, whole genome shotgun sequence.
ACCESSION   DAONFI010000180 DAONFI010000000
VERSION     DAONFI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21912_length_4662_cov_3.834817"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLK08_06630"
     CDS             <1..1017
                     /locus_tag="PLK08_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPS53209.1"
                     /translation="WILRHIHHSDKVLTFSLGMLLLVIAFATRMNMDVILATMTMGVL
                     LINVAPQRSKKLFEVVKQFSGPIYVIFFVLVGARLSLGSMPSWLWWIAGLYVLFRTIG
                     KMFGAWLGAKMSHADKVVCKYTGMGLFAQGGVAVGLSIMASQHLGNLKVGPLTLGDII
                     IFTVTATTLAVQIAGPPAVKLAIKLADEIGRDINEEDILNANKVSDVIKGTPVTIPEH
                     APVADILKIFSDTSEMVLPVIKADGRLAGQISLQNLKNLFARTDCWEWLVAADVMQPA
                     TEVLKADQTLASSLELMREVGVEQILVMDESGQKTCGILDSRTVRQWLEKELIRRQTA
                     VVAA"
     gene            complement(1091..1393)
                     /locus_tag="PLK08_06635"
     CDS             complement(1091..1393)
                     /locus_tag="PLK08_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HPS53210.1"
                     /translation="MIKHIVLWKLKQTADGSTAVENAQIMKERLEALNGRIPGMKKLE
                     VGIDFSRTQASFDVALYSEFESRKALDDYQVHPEHMAVRKFIGGVIEGRHIADYEI"
     gene            complement(1421..3577)
                     /locus_tag="PLK08_06640"
     CDS             complement(1421..3577)
                     /locus_tag="PLK08_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HPS53211.1"
                     /translation="MKQVLQNLRNGETRVADVPAPCLTPGNILIRTHASLISAGTEKM
                     LVEFGKGSLLAKAKAQPEKVKQVLDKIKADGLLPTLEAVFSKLDEPLPLGYCNVGEVV
                     EVGSGVSGFKKGDCVISNGPHAEMVCVPKNLCAKVPDGVTDEEAAFTVLSSIALQGVR
                     LASPTLGEKVVVYGLGLIGIIAVQLLRANGCEVLGVDINAERLAFAEKFGAAVCNGGT
                     ADPIAAAGAWTDGKGVDAVIITASARTDAIVHQAAEMCRKRGRIVLVGVVGLNLNRND
                     FYKKELSFQVSCSYGPGRYDEKYELAGHDYPAGFVRWTEQRNFEAILSLLKTGRLDVK
                     SLITDHFAINDAASAYNRISTNPHTLGVVLKYVEQVDASQVLTVNRFSVTSAGKATVG
                     VIGAGNYSRATMLPILSKTDARIAYIADLDGVPATHLAEKYGVEKAVTDYKVILDDSH
                     IDGVMIAVGHNLHAKFVCEALSAGKHAFVEKPLAMNVDEINRILETMKANPGLQVMVG
                     FNRRFSPHTQKIREKFMGRSEPLAMNMTVNAGIVPPDVWVHDPIRGGGRIIGEGCHFI
                     DLLAYIAGSKVKTVAAVQMSRGVAVKEDKMSIVLGFEDGSVGTVNYFANGDKSYPKEM
                     LEVFCEGKVMRLDNFRETVGYGIRGRRKFRTFRQDKGHAAEYAAFISRIRDGGPEVIP
                     MDEQINITLASFAAMTSAAQNRMIDINAEYGDKIKF"
     gene            3729..4412
                     /locus_tag="PLK08_06645"
     CDS             3729..4412
                     /locus_tag="PLK08_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPS53212.1"
                     /translation="MEQPAFDPITLHSRIAQKFDSRYATVIAFRERLEKWSALIRKWI
                     KPGDSVLDAGCGSGFLTACLSGIAGDITALDGSQEMLNLAKNKFSDEERQKITFVCHR
                     LPLSCDMPIFDAILCSSVLEYLDDMAATLESFYNHLKPGGILILSYPNILSPKRWLDP
                     VAYRLIKKPSFYPYMRNRPKLPGRFAKYLKNFGFEEIQRMYSGRITRINISSLEFILP
                     LFIMTVRKK"
BASE COUNT         1185 a         1253 c         1121 g         1103 t
ORIGIN      
        1 tggatactgc ggcacataca ccactccgac aaggttctga cgttctcgct gggcatgctg
       61 ctgctggtga tagcgttcgc cacgcgaatg aatatggacg taattctggc aaccatgaca
      121 atgggcgtgc ttttaatcaa cgtggcgcct caacggtcga aaaaactttt cgaggtcgtc
      181 aagcagttct ccggcccgat ttacgtgata ttcttcgtac tcgtcggcgc caggctgagt
      241 ctgggcagca tgccgtcgtg gctgtggtgg atagccggtt tatacgtgct gttccgcaca
      301 atcggcaaaa tgttcggagc gtggctcggc gcaaaaatga gccacgccga caaggtcgtc
      361 tgcaaataca ccggcatggg actgttcgcg cagggcggcg tagcagtcgg cctttctatt
      421 atggccagcc agcatctggg aaatttgaaa gtcggcccac ttacgctggg cgacataatc
      481 atcttcaccg tcaccgcaac cacgctggct gtacagattg ccggcccgcc ggctgtcaag
      541 ctggctatca aactggctga cgaaatcggc agagatatca atgaagaaga catcctcaat
      601 gccaacaagg tcagcgatgt catcaaaggt actcccgtaa caatccctga acacgcgccg
      661 gtggctgaca tccttaaaat tttcagcgat acgtcggaaa tggttctgcc cgtgataaaa
      721 gcagacggcc gacttgccgg ccagatttcg ctgcaaaatc tcaaaaatct tttcgccaga
      781 acggactgct gggagtggct ggtggcagcc gacgtaatgc agccggcaac cgaagtcctc
      841 aaagccgacc agaccctggc cagctcgctt gaactcatgc gagaagtcgg cgtagaacag
      901 atactggtaa tggacgaatc cggccagaaa acatgcggca ttctggattc acgaacagta
      961 agacaatggc tcgaaaagga actcatcaga cggcagacag cagtcgtcgc cgcataacga
     1021 gattcataaa gtcggaataa ttttcaaaaa aaggcatggc gtcaaaacca tgccttttta
     1081 acaacgctcg tcatatttcg taatccgcaa tatgccgccc ttctataact cccccaatga
     1141 atttgcggac agccatatgt tcaggatgca cctgataatc gtccaacgct ttgcgggatt
     1201 caaactcgct gtaaagtgca acgtcaaacg acgcctgcgt acgcgaaaaa tctattccga
     1261 cctccagctt tttcatgcca ggaattcgtc cgttgagggc ttcgaggcgt tctttcatta
     1321 tctgcgcgtt ttcgacagcc gtcgagccgt ctgccgtctg tttcaatttc cataaaacaa
     1381 tatgcttaat catttttgaa aaatctccga ttttcatggt tcagaatttt attttatcgc
     1441 cgtattcggc gttgatatct atcatgcggt tctgagccgc cgacgtcatg gcggcaaagc
     1501 tggccagcgt aatgttaatc tgctcatcca tgggaatgac ttcggggccg ccgtcgcgaa
     1561 tgcgggagat gaatgcggca tattcggcgg catggccctt gtcctggcgg aacgtccgaa
     1621 acttccgccg gccgcgaatg ccgtaaccga ccgtctcgcg gaaattgtcg agacgcatga
     1681 ccttgccctc gcagaacact tcgagcattt ccttcggata ggacttgtcg ccgttggcaa
     1741 aataattcac cgtgccgacc gaaccgtcct cgaagcccag tactatggac atcttgtctt
     1801 ccttgacggc tacgccgcgc gacatctgta ccgcggcgac agtcttgact ttactgccgg
     1861 caatatacgc cagcaaatcg ataaaatgac agccttcgcc aataatcctt ccgccgccgc
     1921 gaatggggtc atgcacccat acgtcgggcg gcacaatgcc ggcgttcacg gtcatattca
     1981 tagccagcgg ctcactgcgg cccatgaatt tttcgcgtat cttctgcgtg tgagggctga
     2041 accggcgatt aaaacccacc atcacctgaa ggcccggatt cgccttcatg gtttccagaa
     2101 tgcggtttat ctcatcgaca ttcatagcca gcggtttttc tacaaaagcg tgtttgccgg
     2161 cagaaagcgc ttcgcagaca aatttggcgt gcaaattatg gccgacggct atcatgacgc
     2221 cgtcaatatg agaatcatca agaataactt tataatccgt aacagctttt tcaacgccgt
     2281 atttttcggc cagatgcgtc gcaggcacgc cgtccaggtc tgcaatatag gcaatgcggg
     2341 catccgtctt cgataaaatc ggcagcatgg tggcccgtga ataattgcct gcgccgataa
     2401 cgcccaccgt agccttgccc gccgacgtca cggaaaacct gtttaccgtc agaacctgag
     2461 atgcatcgac ctgttcaaca tatttaagca ctacgccaag cgtgtgagga tttgtcgata
     2521 tgcgattata agcggaggct gcatcgttga tggcgaaatg gtccgtaata agagatttca
     2581 cgtcaagccg gccggttttc agcagtgaaa gaatcgcctc aaaattccgc tgttccgtcc
     2641 aacgtacaaa acccgccgga taatcatggc cggcaagttc gtatttttcg tcataacggc
     2701 ccggcccata tgaacatgag acctggaaag agagttcctt cttgtaaaaa tcgttgcggt
     2761 tgaggttaag gcccactacg ccgacaagca cgatacggcc gcgtttacgg cacatttcgg
     2821 cagcctgatg cacgatggca tcagtcctgg cactggcggt gattatgacc gcgtcgacgc
     2881 ctttgccgtc cgtccacgca ccagccgcgg caatcgggtc ggctgtgccg ccgttgcata
     2941 cggcagcgcc gaatttctct gcaaacgcca accgctcggc gtttatgtcc acacccagca
     3001 cctcacagcc gttagccctg agcaactgta cggctataat cccaataagc ccaaggccgt
     3061 aaacaaccac tttctcgcca agcgtcggcg atgcgagtcg tacgccctgc agggcaatgc
     3121 tggacaggac agtaaacgct gcttcctcgt cagtaacgcc gtccggcact ttcgcacaaa
     3181 gatttttcgg cacgcaaacc atctcggcgt gcgggccgtt ggatatgaca caatcgcctt
     3241 ttttgaaccc ggatactcca gagccaactt ccacaacttc accgacgtta cagtaaccca
     3301 acggcaatgg ctcgtcgagt ttgctaaaaa ctgcttcaag cgtcggcagc aagccgtctg
     3361 ccttgatttt atcgagtacc tgtttgacct tttccggctg agccttagcc ttagccagca
     3421 ggctgccctt accgaattcc accagcattt tttccgttcc ggcggaaata aggctggcat
     3481 gagtacgtat cagtatattg ccaggagtca ggcacggcgc cggcacatcg gctacacggg
     3541 tttctccgtt acgaaggttc tgcaaaacct gtttcatcag gaaatcctct tttcatatac
     3601 atcagggaag tcattacgtt gaaaaaacga cagtatagtt atatacttta ttaactgttt
     3661 tggaagaatt attttcccgt atcttccatg ttagccgtaa aatggccgcc agccataagg
     3721 aattacatat ggaacagcca gcattcgacc cgataacact tcattccaga atcgcacaaa
     3781 aattcgacag ccgatacgcc accgtaatcg cgttccgtga acgtctcgaa aaatggtcgg
     3841 cattaattcg caagtggata aaacctggcg acagcgttct ggatgccggc tgcggcagcg
     3901 ggtttctgac ggcttgtctt tcgggaatag ctggagatat aaccgcactg gacggctcac
     3961 aggagatgct gaatctggca aaaaacaaat tttcagacga agaacgccag aaaatcactt
     4021 tcgtctgcca tcgcctgccg ctatcctgcg acatgccgat atttgacgcc atcctgtgtt
     4081 caagcgtatt ggaatacctg gatgatatgg cggctacgtt agagtcgttt tataaccacc
     4141 tcaagccggg cggcattctc atcttatcat atccgaacat tctgtcgccg aaacgctggc
     4201 tggacccggt ggcgtacagg ctcatcaaaa aaccgtcgtt ttatccgtat atgcgcaatc
     4261 ggccgaaatt gccgggccgg tttgctaaat atcttaaaaa tttcggcttc gaggaaattc
     4321 agcgcatgta cagcggcaga atcactcgca taaatatctc cagcctggaa ttcatcctgc
     4381 cgctattcat aatgacagtc agaaaaaaat gacatatgcc aaagcatggt aaaactaggg
     4441 agcgttctca attaaactgt gagacgtaaa tgagtagcaa ttgtgcaaat gcaagaagaa
     4501 aagaggctgc atatttgttg gatattctac ctttcttctg acgaagcaga tgcttcatca
     4561 ccacaagcag ataaagacaa aatactcaaa acgtaatact aggacttttt taacgaatac
     4621 ttgtcacgga aaatagatga aacaatctgg cgaaatttcc ca
//
LOCUS       DAONFI010000181         4656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_21950_length_4656_cov_3.606825, whole genome shotgun sequence.
ACCESSION   DAONFI010000181 DAONFI010000000
VERSION     DAONFI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21950_length_4656_cov_3.606825"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1465)
                     /locus_tag="PLK08_06650"
     CDS             complement(257..1465)
                     /locus_tag="PLK08_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53213.1"
                     /translation="MAGLFFKRIASVIFVIVSFYVPVVAAPPANPDIPSDLLREKLLS
                     TYVNQMFQRAKYWRSDICRASSVDGIFAVREEINKDYNLYKVENYQKEYAKITEALYL
                     PILQGKLNLIKSNDPLAVVKLINIALVISDMNPDYTARGFEEMLASPNQAICYIGWQG
                     MKKSRNSETGASQFKILYTSAQSTLSKETNATILSEVMQAIDFGTAATQTVPEKYRKL
                     AAGKTVELITQNWKKLTADVSAANAGEKLAVPAMAITTLANNATFTGDDKTKKQAIAM
                     IYAQAQTAVARIEKLQAAAKTLKNRLDRLKASLEDRTSENMNQTANKNSSQELTPEQK
                     KLSYKLDEAETLAGDYKVLMMNCEDAFNTLTGASNDFIAKQMTASADPAAAMKLGLLD
                     WKSVLKPDAK"
     gene            1587..2477
                     /locus_tag="PLK08_06655"
     CDS             1587..2477
                     /locus_tag="PLK08_06655"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPS53214.1"
                     /translation="MSRKSTTISVIGPGRLGGTLACAVAAAGYDVKALGVRHVTDAAR
                     ELADAVGAALLSPTEAADAADAVFLCCGDDAVENLCQTLVRQGGFLGKNGQYVIHCSG
                     ALDSGVLDAAEKCGCMTGSFHPLQTFPDVQAGLARWAGTHCFIETGYPELREFLHELA
                     VKTGAVPHDIDAGQKTLYHAAAALCCNHMTALIDVAMRLFEKTGLGYDEALTAAGPIM
                     RATLENILSKKGSAGALTGPIARGDVETVKKHLNALNSLPETADVYRSLGMATVSLAQ
                     RGGLISDDKAATLRRILDER"
     gene            2486..3466
                     /locus_tag="PLK08_06660"
     CDS             2486..3466
                     /locus_tag="PLK08_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPS53215.1"
                     /translation="MADEKIIDGRAIAREIKDAVRAAAVEFRAAHGRPPKLAAIQVGE
                     CSASQVYTNMQAKNAAECEMDYDLRIVPADVDDAKLLLEIGSLNDDSAVDGIILQMPL
                     PPHFDASVAQAAISSRKDVEGVAYDTMGRIFFGCNLIAPCTPMAVMEILHRFCEPLAG
                     KEVVLIGASEIVGKPLAMLMLQRHGKMPTLTICSSRTRDLSAHTLNADIVITAAGVSQ
                     IRWRNYKNGGCVAPAPDLNPLVCGDMLKDGAIVIDVATNRVPAGFDMSGQPRKTADGK
                     LDMHTVGDVDFESALRKVSKITPVPGGVGPVTVAILLRNTLACAENNMNA"
     gene            3501..>4656
                     /locus_tag="PLK08_06665"
     CDS             3501..>4656
                     /locus_tag="PLK08_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53216.1"
                     /translation="MKMGLPCCVFLLLSAVSLYGQPLDIEKQYYAVFVGPAKIGYIRT
                     TRTVEGGRVITASLLKISAQRDKFRITAKVSIREVETADGKPLSFRTVKEYSGTRSEI
                     CGEIQPDGKLKMTVKSGGDSKKMKIVTWPAGSVMSEGARLIAMKKGLAPGTKYDVLEF
                     DADSCTARKASITVGELQNVRLTDRTTKLHKITQMMIFGSSVIGTTLYVDDECNAMKT
                     VTGMMGMDMTMVACDRSFALSPCNREVDFLKKLSIPSPVDIPDNAVKAIYLIQPLQNM
                     TLKFPQIANQTVKKKGKLYEVTVTAVEMSASAEIPYKGQNRAAIKALKPSEYVQSDSP
                     EIRKLADQAVGDAKNAATAAVRIRNFVRGYITHKDLSVGFASAMEVARSRT"
BASE COUNT         1103 a         1080 c         1288 g         1185 t
ORIGIN      
        1 ctgtttttta tccgagttgt caagcagtac gaacggcaca agcgtcagtg ccgcgacgac
       61 aagtaccgaa atgacgtaaa atagcttcat ctggatatat taaattttct ataggttaaa
      121 atcattagca tggtatttat caggttttca cctgtccgcc ggctgacggt tgatttcatc
      181 tacagtttat aatagtgcga aaaaaatcgg cgacactttt ttaaaaaccg tagaattacg
      241 aaggacatat ttaaaattac ttggcatccg gcttgagaac cgatttccag tccagcaggc
      301 ccagtttcat agctgccgct ggatcggctg atgcggtcat ctgcttcgcg atgaagtcat
      361 tcgatgcgcc ggttaatgta ttgaatgcgt cctcgcagtt catcatcaat accttataat
      421 ctccggcgag ggtttctgct tcatcgagtt tgtaggagag ttttttctgt tccggcgtaa
      481 gttcctgcga ggaattttta tttgccgtct ggttcatatt ttcagaggtt ctgtcttcga
      541 gagacgcctt gagtcggtcg agtctgtttt tgagtgtttt ggcggcagcc tgcagttttt
      601 ctattctcgc tacggcggtt tgtgcctgcg cataaatcat ggcgatagcc tgtttttttg
      661 ttttgtcgtc gccggtgaag gttgcgttgt tggccaatgt ggttatcgcc atcgccggca
      721 cagccagctt ttcaccggca ttggctgccg atacgtcggc agtgagtttt ttccagtttt
      781 gcgttataag ttccacagtt ttgccggctg cgagttttcg gtatttttcg ggtacagtct
      841 gcgttgccgc cgtgccgaaa tcgatagcct gcatgacttc ggacagaatc gtcgcattcg
      901 tttccttcga aagcgttgac tgggcagatg tgtacaggat tttaaactgc gatgcgcctg
      961 tttcggaatt gcggcttttt ttcattccct gccagccgat gtagcagatt gcctgattcg
     1021 gactggcgag catttcctca aaacccctgg cggtatagtc aggattcatg tcggaaatta
     1081 cgagcgcgat gttgataagt ttcacgacgg cgagcgggtc gttggatttg atgagattca
     1141 gtttgccctg aagtatcggc agatacagcg cttctgtgat tttggcgtat tctttctgat
     1201 agttctccac tttgtagagg ttgtaatctt tatttatctc ttcacggacg gcgaagatgc
     1261 cgtctacgct cgatgccctg cagatatcgc ttcgccagta ctttgcacgc tggaacatct
     1321 ggttgacgta ggttgaaagg agtttttctc tgagcaggtc tgacggtatg tcgggatttg
     1381 ctggcggggc tgcgacgacg ggtacgtaaa atgacacaat tacaaaaatg acgcttgcaa
     1441 ttcgtttaaa aaaaagtcca gccatggttg tcatccttaa atatcaggta aaaaacaaat
     1501 ttacagcggc atcaatagta aacaaaccgg ccgctttcgt caagaatcta ttgccacggg
     1561 gtgacgaaaa ctatatcttc tgtgacatgt cccgaaaatc gactacaata tctgtaatag
     1621 gccctggccg tctcggcgga actctcgcat gcgctgttgc ggcggcagga tatgatgtga
     1681 aggctttggg ggttcgtcat gtgaccgacg ctgccagaga gctggctgat gcggttggtg
     1741 cggcattgct ttcgccgacg gaagcggctg atgccgccga tgcggttttc ctgtgctgcg
     1801 gcgatgatgc cgtggaaaat ctctgtcaga ccctcgtccg acaggggggc tttttgggaa
     1861 aaaacggcca atatgtcata cattgcagcg gggctctgga tagcggggtt ctcgatgctg
     1921 ccgaaaaatg cggatgcatg accggcagtt ttcatccgtt gcagactttt ccagatgttc
     1981 aggctggcct ggcgagatgg gccggcacgc attgtttcat cgagaccgga tatccggaat
     2041 tgcgtgaatt tttgcatgaa ctggcggtta agaccggagc tgtgccgcac gacatcgatg
     2101 ccggccaaaa aacgctttat cacgcagccg cggcgttgtg ctgcaatcat atgacggcgc
     2161 tgattgatgt ggctatgaga ctttttgaga agacagggct tggatatgac gaggcgttga
     2221 cggcggctgg cccgataatg cgggcgacgc ttgagaatat tttatcaaaa aaaggctcag
     2281 ccggcgcatt gaccggcccg attgcccgcg gcgatgtcga aaccgtaaaa aaacatctga
     2341 acgccttgaa tagcctgcca gaaacggctg acgtttaccg ctcgctcggc atggctaccg
     2401 tcagcctggc acagcgaggc ggattgatat cggatgacaa agctgcgacg ctgcggcgta
     2461 ttctggatga aaggtaagaa atttgatggc tgatgaaaag attatcgacg gcagggcgat
     2521 agcccgtgaa ataaaggatg cggttcgtgc ggcggctgtt gaattcaggg ctgctcacgg
     2581 tcggccgccc aagctggcgg ctatacaggt cggcgagtgt tccgccagcc aggtttatac
     2641 gaatatgcag gctaaaaacg cagccgaatg cgaaatggat tacgacctgc gaatcgtgcc
     2701 ggctgacgtt gacgacgcga aactactgct tgaaatcggg tcgctgaacg acgattctgc
     2761 ggtggacggc attattctcc agatgccgct gccgccgcat ttcgacgcgt cggtggcgca
     2821 ggctgcgata agttcacgca aggatgttga gggcgttgca tatgacacga tgggccgtat
     2881 tttcttcggc tgcaatctca ttgccccgtg tacgccgatg gcggtaatgg aaatacttca
     2941 tcggttctgc gagccgttag ccggcaagga agtggtgcta atcggggcga gtgaaatcgt
     3001 cggcaagccg cttgctatgc tgatgttgca gcggcatggg aaaatgccga cgcttacgat
     3061 ttgcagctcc cgtacgcgtg acctttctgc tcacacactc aatgccgata tcgtcataac
     3121 ggcggcaggc gtttcccaga ttcgctggcg gaattataaa aacggcggct gtgttgcgcc
     3181 ggcgccggat ttaaacccgc tcgtctgcgg cgatatgctc aaggacggcg cgattgtcat
     3241 agacgtggct accaaccgtg tgccggcggg tttcgatatg agtggccagc cgcgcaagac
     3301 ggctgacggc aaattggaca tgcataccgt cggcgacgtt gattttgaaa gcgcattgcg
     3361 gaaagtgtcg aaaataacgc ccgtccctgg cggagtaggc cctgtgaccg ttgccatact
     3421 tttgcgaaac acccttgcat gtgcggaaaa caacatgaat gcatagatgt agctgtcgga
     3481 caactgtttg aggattaata atgaagatgg gacttccatg ctgcgttttc cttttactgt
     3541 cagccgtcag cctttatggt cagccgttgg atattgaaaa gcagtattat gcggtttttg
     3601 tcgggccggc taaaatagga tacatccgta cgacaagaac cgtcgagggc ggcagggtaa
     3661 taacagcctc actgctcaag atatccgctc aacgtgacaa attcagaatt actgcgaaag
     3721 tttcgatacg cgaagttgaa acggctgacg gcaagccgct gtcttttcgt accgtgaaag
     3781 aatattccgg aactcgcagc gaaatttgcg gcgagattca gccggacggc aaattgaaga
     3841 tgaccgttaa atccggcggc gatagtaaaa aaatgaaaat cgttacatgg cctgccggtt
     3901 ctgtgatgag cgagggcgcg agactgattg cgatgaaaaa gggccttgcg cctggaacta
     3961 aatacgacgt tcttgaattc gatgctgatt catgcacagc caggaaagcc agcattacgg
     4021 tcggagaatt gcagaacgtc cgtctgaccg accggacaac aaagctgcac aaaataacgc
     4081 agatgatgat tttcggcagt tctgtcatag gcactactct ctatgtcgat gatgagtgca
     4141 atgcgatgaa gacagttacg ggcatgatgg gaatggatat gaccatggtc gcctgtgacc
     4201 gcagttttgc gctgagtcca tgcaaccgcg aggtcgactt cctcaaaaaa ctgtccattc
     4261 catcgccggt cgatattccc gacaatgccg taaaagccat ctatttaatt cagccgctac
     4321 agaacatgac actgaaattc ccgcagatag ccaatcagac tgtcaagaaa aaaggcaagt
     4381 tgtatgaagt caccgttact gccgttgaaa tgtcagcatc ggctgaaatt ccgtacaagg
     4441 gtcagaatcg ggcggctata aaggctctaa aaccgtccga gtatgttcag tccgactcgc
     4501 ctgaaatcag aaaactggct gaccaggccg tgggggatgc aaaaaatgca gcgactgctg
     4561 ccgtgagaat cagaaatttt gttagaggtt atataacgca caaagacctg tccgtcggtt
     4621 ttgcctctgc tatggaagtg gcccgcagca gaacgg
//
LOCUS       DAONFI010000182         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_22009_length_4648_cov_3.819943, whole genome shotgun sequence.
ACCESSION   DAONFI010000182 DAONFI010000000
VERSION     DAONFI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22009_length_4648_cov_3.819943"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLK08_06670"
     CDS             complement(<1..180)
                     /locus_tag="PLK08_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPS53217.1"
                     /translation="MEKKLLSGNEAVALACRDYGVHMGTGYPGTPSTEILENFSELGG
                     YAEWSPNEKVAMEVAA"
     gene            complement(272..1816)
                     /locus_tag="PLK08_06675"
     CDS             complement(272..1816)
                     /locus_tag="PLK08_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HPS53218.1"
                     /translation="MEKICELILVAAAASVCTFAAAETKTSTTQPAGQPEQQNFEESL
                     KDIITVPADILIGIPKLFVKLIDAPQEAIIQPNPTETGLYKYDPAEIAYELQDREGTR
                     EIVEKKWFKWGFDFRAREVYHKNAVYLDSHRPNSEYQFQRYRSRIWSTITPVEGVDIN
                     SRLTWEFRHYCEPHSVDSDPNSEAVIDTLNVTFTKPADLPAKFIMGRQDIFIGDGWLI
                     ADGTPLDGSRTYYFDAFRLQYNFDNENDLDVMYLNTPANSDQYITPFNSQHSYLVEQN
                     MQGVATIWNNRSIIKGSTLSPYIIWSKDNKVLSSGYNADLYTFGARASGNLRANWFYR
                     AEAAMQLGRKDGQAVAAGGLNSLLEYRFLDGWQNSIGLGYEYLSGDDPDSSTNEQFDP
                     LWGRWPRFSELLIYTFSGETHLAEATNMHRLSLGWTAHPHKKIELYSTYQLLFAAQNT
                     FRDKEGFSENGKFRGQLVTALLKYKFNENISSHIVGEVFFPGDYYDDSRNDPAVFVRY
                     QLLFEW"
     gene            2143..>4648
                     /gene="secD"
                     /locus_tag="PLK08_06680"
     CDS             2143..>4648
                     /gene="secD"
                     /locus_tag="PLK08_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPS53219.1"
                     /translation="MEGKNLWAKFGFVFVLLAVSVCAILVYGLKRGPDIAGGYSMIFE
                     VENPANDSKMVEDIIGILKERVDPTGLSSIEWRPLGSSRFEVRMPETSDKSKKYRTEF
                     NKNVETLLATNIERADIRTIMNTPVGQRDVKINTLVEGDKARAVAVKALIASIEKLNQ
                     TENTLADVKAQAADARNSGASRRKELQAKLLDAMKAQDEALSDYTAKLDAVRKGNISS
                     RDLFSLFRGYDKAMAQNVDEKRSTAMDSFNDRAEMMQEDHPAQAGRLAALIALYKEWS
                     EIKSPLEDPNDLKRMIRKSGVLEFRIVPFKPGYNSQSVLTQKQVDEYTRTLMDKGPDV
                     VIANGDYVWMPLRDEGEKYDSVVVADAGGTKYMLLSNKPGETMLQNRKGNKTWQLDSS
                     RASSDNFGKPAVNFTFDARGAKLFGILTSAHKSDPGTGREGMPMAILLDDEVYSAPRI
                     QSTITSSGQITGNFSLQEVQELVRILKAGSLPGKVKPEPISESSFSAALGKVNIEKGI
                     RSAIYGTMAVAVFMFIYYLLAGLVADVALMMNIILVLGAMSLLQAAMTLPGIAGVILT
                     IGMAVDANVLIYERLREEQNRGLAIRQALKNAYERAFSAIFDSNITTLLICAFLYFVF
                     GELGMGEVRGFAITLGLGVMFSMFTALVVTRWVFQLLMDTRILRKPIKMLTLVPIAKI
                     NWISKRYYFWTVSAALIITGIVPLAWQGSNIWGIEFSSGTQVMLILKDDTLLNGRLPQ
                     DEIVRASFGNEAGTLLADADDKLKLEIGKLSGTATRVEAKLADNRIGDFIANYGNDKG
                     QVTLDDWKKKHKNIAFFEVLDKDRNGILSEVELQNLP"
BASE COUNT         1091 a         1119 c         1276 g         1162 t
ORIGIN      
        1 ggcggctact tccatagcca ccttttcgtt cggcgaccat tccgcgtatc cgcccagttc
       61 gctgaaattt tccagtatct ctgttgacgg cgtgccaggg tagcctgttc ccatatgcac
      121 tccgtaatct cggcaggcta gtgcgacagc ctcgttaccg ctgagtaatt tcttttccat
      181 cttcagaaca actccaaatt tttttaaaaa atcaaaaact gtgtccccga aaccttcagt
      241 cgaccggcgg ctgcgttttc agattgtcag cttaccattc gaacagaagc tgatatctta
      301 cgaaaacggc cgggtcattg cgagagtcgt cataataatc tccggggaaa aatacttcgc
      361 cgacaatatg actgctgatg ttttcgttga atttgtattt cagcagagcc gtcacaagct
      421 ggcctctgaa tttgccgttt tcggaaaacc cctctttgtc gcgaaatgta ttttgagccg
      481 caaaaagaag ctgatatgta gaatatagtt cgattttctt atgcgggtgg gcggtccagc
      541 cgaggctcag ccggtgcatg ttggtggctt ctgcgaggtg agtttcacct gagaacgtgt
      601 aaatcagcag ttcgctgaag cggggccatc ggccccacag cgggtcgaac tgctcattgg
      661 tcgacgaatc ggggtcgtcg ccggagagat actcgtaacc gaggcctatc gagttctgcc
      721 agccgtccag gaagcggtat tccagcaacg aattcaagcc gccggctgcg acggcctggc
      781 cgtctttgcg gcccagctgc atggctgctt ccgccctgta gaaccagtta gcccttaaat
      841 tgccggatgc acgcgcgccg aacgtataca ggtcggcgtt atagccgctc gatagcacct
      901 tgttgtcttt cgaccagatg atgtatgggc ttagcgtcga accttttatg atgcttctat
      961 tgttccagat tgtggcgacg ccctgcatgt tctgttcgac gaggtatgag tgttgtgagt
     1021 tgaagggtgt tatatattgg tcgctgttag ccggggtgtt taggtacatg acgtcgaggt
     1081 cgttttcatt gtcgaaatta tattgcaggc ggaatgcgtc gaagtagtac gttctggacc
     1141 cgtccaatgg cgtgccgtcg gcaatcagcc agccgtcgcc tatgaagatg tcctgccggc
     1201 ccatgatgaa ttttgccggc aaatccgcag gtttggtgaa tgtaacattg agcgtatcta
     1261 tgacggcttc gctgttgggg tcggaatcta cgctgtgcgg ctcgcaatag tgacggaatt
     1321 cccacgtcag gcgagaattt atgtccacgc cttctaccgg cgttatggtg gaccatattc
     1381 gcgaacgata ccgctggaac tgatattcgc tgtttggccg atggctgtcc aggtacacgg
     1441 cgtttttgtg atatacctcg cgggctctga agtcgaaccc ccatttgaac cattttttct
     1501 ccacgatttc tcgtgtgcct tcgcggtctt gcaattcgta ggctatttcc gcggggtcat
     1561 atttgtataa acccgtttct gtcggattgg gctggattat tgcttcctgt ggggcgtcta
     1621 tcagtttgac gaaaagtttg ggtatgccga ttaaaatatc tgccggcaca gttatgatgt
     1681 ctttgagcga ttcttcgaaa ttttgttgtt caggctgtcc agccggctgg gttgtggatg
     1741 ttttcgtttc agcggcggcg aaagtgcaga cggacgctgc ggctgcaacg agaatcagtt
     1801 cacatatttt ttccattaaa ataatccttg gcccaaagta gtaaatcttg aggcatgtga
     1861 aagagcagta agcgtccttt ccacaggctg ggaaaagcct gaaaacgaaa gttatgaggg
     1921 gatttttact tgtaacaagc tgtttgtcaa gcaattttgg gcatttatgg cgttcgggcc
     1981 ggactttatc tgaaattgac aaataaaatg gcggcataag gcaggaaatt ctaaaaaaac
     2041 ttgactgaaa ggaagatatg atagaaaatc tccgcttcgg tctgctggct gattatgcgc
     2101 cgatgcaggc tggtttgtgt ctattttttt aaaggatact ttatggaagg caagaatctt
     2161 tgggccaagt tcggttttgt gtttgttctg ctggcagtaa gcgtttgtgc catacttgtt
     2221 tatggcctta agcgagggcc cgacattgcc ggcggctaca gcatgatatt cgaggtggaa
     2281 aaccccgcca acgacagcaa gatggtggaa gatatcatcg gcattctcaa ggaacgagtc
     2341 gacccgactg gccttagcag catagagtgg cggcctttgg gcagcagccg ttttgaagta
     2401 cgtatgcccg aaacgtccga caagagcaaa aagtatcgta ccgaattcaa caagaacgtc
     2461 gagacgctgc ttgcgacgaa tatcgaacgt gccgacattc gtactataat gaacacgcct
     2521 gtcggccagc gtgatgtgaa aatcaatacc ctcgtagagg gcgataaagc ccgcgccgta
     2581 gcggtaaaag cactcatcgc atccatagag aaactcaatc agacagaaaa tacgctggct
     2641 gacgtaaagg ctcaggctgc cgatgcaagg aattccggcg cgtcgcgaag aaaagaattg
     2701 caagccaaac tgcttgacgc catgaaggct caggatgaag cactgagtga ttataccgcg
     2761 aagctcgatg ccgtgcgcaa gggcaatata tcctctcgcg accttttcag ccttttccgt
     2821 gggtatgaca aggctatggc gcagaatgtg gatgagaaac gcagtacggc aatggacagc
     2881 ttcaatgaca gggcggagat gatgcaggaa gatcatccgg ctcaggctgg tcggctggct
     2941 gcgctgattg ccctgtataa ggaatggtcg gaaatcaagt ctccgctgga agaccccaac
     3001 gacctcaaac gcatgatacg caagtccggc gtgcttgaat tcaggattgt gcctttcaaa
     3061 cccggctata acagccagtc tgttctgacg cagaaacagg ttgacgaata tacccgtacg
     3121 ctgatggata aaggccctga cgtcgtcatt gccaacggcg attacgtctg gatgccgctt
     3181 cgcgatgagg gtgaaaaata cgattccgtc gttgttgccg atgctggcgg caccaaatat
     3241 atgctgctga gcaacaaacc cggcgagacc atgcttcaga atcgcaaggg caacaagacg
     3301 tggcagttgg attcctcccg cgcaagcagc gataatttcg gcaagccagc cgtcaatttc
     3361 accttcgatg caaggggcgc caaactgttc ggaattctga cgtcggcgca caagtccgac
     3421 cctggcacag gcagggaagg catgccgatg gctattttgc tggatgacga agtctattcg
     3481 gcgccgcgca tacagtcgac cattacgtcg agcggccaga taacgggcaa tttctcgttg
     3541 caggaagttc aggaactcgt ccgcatcctc aaagccggct ctctgcccgg caaggtcaag
     3601 cctgagccaa tcagcgaatc gtcattttcc gccgctctgg gcaaggtcaa tatcgaaaag
     3661 ggcatacgct cggcgattta cggcacgatg gcggtggcgg tgttcatgtt catatattac
     3721 ctgctcgccg gcttggtggc tgacgtggcg ctgatgatga acattatcct ggtgctcgga
     3781 gcgatgagcc tgcttcaggc ggctatgacg ctgccaggca ttgcaggtgt cattctgacc
     3841 atcggcatgg cggttgacgc gaacgtgctt atttacgagc gtttgcggga agaacagaac
     3901 cgcggcctgg ccattcggca ggccctcaaa aatgcctatg aacgggcgtt ttcggcgatt
     3961 ttcgactcga acataaccac gctccttatt tgtgcgttcc tttacttcgt gtttggcgag
     4021 ctgggcatgg gcgaggttcg cggctttgcg attacattgg gcctgggcgt catgttcagc
     4081 atgttcacgg ctctggtggt tacgcggtgg gtgttccagc tgttgatgga tacgcgtata
     4141 cttcgcaagc ccataaaaat gctgacgttg gttccgattg caaagataaa ctggatatcc
     4201 aaacgttatt atttctggac cgtatcagca gccttgataa taaccggcat agtgccgctg
     4261 gcgtggcagg gcagcaatat atggggcata gaattttcca gcggtacgca ggtgatgctg
     4321 attctgaagg acgatacgct tttgaatggc cgtctgccgc aggatgaaat agtccgcgcg
     4381 agtttcggca atgaggccgg cactcttctc gcagacgccg acgacaaact caagctggaa
     4441 atcggcaaac tctccggcac ggctacccgc gttgaagcca agctcgccga caacagaatt
     4501 ggcgatttta ttgcgaatta cggcaatgac aaaggtcaag tcacactgga tgactggaag
     4561 aagaagcaca aaaatatagc cttctttgaa gttttggaca aagaccgcaa cggcattctg
     4621 tcggaggtgg aattgcagaa tctgccgc
//
LOCUS       DAONFI010000183         4632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_22111_length_4632_cov_3.967664, whole genome shotgun sequence.
ACCESSION   DAONFI010000183 DAONFI010000000
VERSION     DAONFI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22111_length_4632_cov_3.967664"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLK08_06685"
     CDS             <1..386
                     /locus_tag="PLK08_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS53220.1"
                     /translation="PVSFAQAGAYAAYTDPRAAETIEKMRAEFERRGKHMAARLNTMP
                     GVKCVEPQGAFYCFPDISSHYGKTMAGVEVKDSLSFARAALEGVQVAIVPGVAFGEDR
                     CIRLSFATSIEQIDKGLDRLEKLLK"
     gene            complement(643..1902)
                     /locus_tag="PLK08_06690"
     CDS             complement(643..1902)
                     /locus_tag="PLK08_06690"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPS53221.1"
                     /translation="MSADTILRNAWANFRKAKEMLSSEYSEELLEKVGEPHERIEMRL
                     APMFDDGKLHAFRAYIVRHSQDLGPSKGGIRMAANVTLDDINGLAMEMTWKCALIGVP
                     FGGGKSGIVADPRKLSAYNKETLIRSFTRSASRHIGPHIYVPAPDMGTGEKDMGYIKD
                     TIAYSAGTANTDGCYVTGKPVILGGIPGRREATGRGVVMTLMASLEQKGMDIRKIRAI
                     VQGFGNVGSVAAEVLHELGATVIAISDIDGGTFNADGLDVPELLTHVKETGSIANFPG
                     GENVDGKAMLEMDCDVLVVAAAANQIVDENARKIKAKIIAEGANSPTSPEADKILAER
                     GIFVIPDILCNAGGVFVSYLEYTQETQQEQMTLLEVQERLARRMTERFNVVWDMAGHK
                     KCSMRDAAMYVAVRNVCDAVQARGFLP"
     gene            complement(2237..>4632)
                     /locus_tag="PLK08_06695"
     CDS             complement(2237..>4632)
                     /locus_tag="PLK08_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HPS53222.1"
                     /translation="LAIGTTGKGTLSATNGSTINISDYLYLTGTNFVDSSVTLEDSTL
                     VIDEDNESYGLYIVTSKYGGLNFSATNCDITVGSITVNISNTTVTSDDTNYPNSSMST
                     VTFDGTSAIEVTGDVSFSGSYYTATISQPMNVGGDFTVSGNGASTLTVSGDDTAVAID
                     GALVVNSTSGFSVLDNSTMTAGTLEMGENAQIVVTDTSGSGAVAIGDSVTAVNGAVVV
                     GADIYDEDEDEYIPLMTVDGGFSVIGNLIMYGTMGTEGASVAEYTTTTGYTLKDGATL
                     VVNIDTDASTINSDTLAVTDGDVILEDGSTVYVSVNGSSIMSLFLEDYFDVITAGGTI
                     TDNASIKCSSLFFDFNSEIVTSGTDEVYRLNLSQTSGFSGPAVGENEKSTAGALDAML
                     ADALDDIQHEDDDDYEDKVTAAQKTMLNNLLTFDDVDSYNYALKQMDGEMYTMHGTNL
                     PNQMESFNRFISQHLTQMRNGNNRQVTRVADSSLGMSLATTDIDPETMAEIIEGKQEE
                     EAREYALSVPDSTDSTKWSVWGQIINMWHKEKASVELTGYNNSAWGAVVGTDRKFAPG
                     LRIGALIGYIRSEYHMNDSRGKLDVDTLRVGPYIDWSVGKLFIEGTLTFGYHWNDAER
                     QVNYDNFSDVDTADDYGAWDVTPYARVGYRFNVAGWDLTPSMALQYVHYNQNGTNETG
                     GAAALEVGDYTNDSFRQIFMVEISKLFDLKYVKLVPAVSLGYSYDYLNPKALDAGFVG
                     GYPMTFYTDSTSKGHFIWGVSVTALLSDDLSLFLRYDGFAAGSDYSNALSSGVNYKF"
BASE COUNT         1196 a         1247 c         1018 g         1171 t
ORIGIN      
        1 atcccgtaag tttcgcacaa gccggcgctt acgcagccta taccgacccg cgggcagctg
       61 aaacaataga aaaaatgcgg gctgaattcg agcggcgcgg caaacatatg gcagcccgcc
      121 tgaacacaat gccaggcgta aaatgcgtag aaccgcaagg cgcattttac tgtttcccgg
      181 acatctccag ccactacggc aaaaccatgg ccggcgtaga agtcaaggat tcactgagct
      241 ttgcgagagc agccctcgaa ggcgtacagg tagccatagt accaggcgta gccttcggcg
      301 aagacagatg catacggctg agtttcgcaa caagcatcga acagattgac aaaggcctgg
      361 atcgcctgga aaaactgctg aaatgaatta aattagaaca tagattgaca tagtgtccga
      421 taatgtaaaa atttttcgaa attttttatt tttaggtagt ctggctgtgt cggcttgcta
      481 gaatatgtat ataggctcct cggtcatgga aaacagctct caactatgac cgttccggcg
      541 tcggactccc ctcctccgac gccttttttt tgcgcttttt tacaaggttt agagccgttc
      601 ccgaaagaat tttttatcgg gaactgctct aaaagtccaa tcctacggca gaaaaccgcg
      661 agcctgcact gcatcacaga cgttacggac tgcgacatac atagcagcat cacgcattga
      721 gcattttttg tggccagcca tatcccacac cacattgaac cgttcagtca tacgtcttgc
      781 cagacgttcc tgaacctcaa gcagtgtcat ctgctcctgc tgcgtttcct gcgtatactc
      841 cagatacgac acgaacacgc cgccggcatt acagagaatg tccggaataa caaaaatacc
      901 tctttctgcc aatattttat cagcctcagg gcttgtcggg ctattggcgc cttctgcgat
      961 aattttggcc tttatcttcc gggcgttctc gtcgacaatc tgattagccg cagccgccac
     1021 aaccaaaacg tcacaatcca tctcaagcat ggctttgccg tcaacattct cgccgccagg
     1081 gaaattcgca atagagccgg tttcttttac atgcgtcaga agctccggca catcaaggcc
     1141 atcggcgtta aacgtaccgc cgtcgatatc cgatatcgca ataaccgtag cgccgagttc
     1201 atgcaatacc tcggcagcca ccgagcctac attgccaaaa ccctgtacga tggcgcgaat
     1261 cttgcggata tccatgccct tctgttccag cgatgccata agcgtcatca caacgcctct
     1321 gccggtcgct tcacggcggc ccggaatgcc gcccaatatg acgggcttgc cggtaacata
     1381 gcagccgtcc gtattagccg tgccggcaga ataggcgatg gtatccttga tgtatcccat
     1441 atccttttcg ccagtgccca tatcaggcgc aggtacgtat atatgtgggc ctatgtgccg
     1501 tgacgcacta cgggtaaagc tgcgaatcag cgtctcctta ttgtaggcgg acagcttacg
     1561 cgggtcggct acgataccgc tcttgccgcc gccgaaaggt acgccgataa gtgcacattt
     1621 ccacgtcatc tccatcgcaa ggccgttgat atcgtccagc gtaacattag ccgccatgcg
     1681 tatcccgcct ttgctggggc ccaaatcctg gctgtgacgg acgatatatg ccctaaaagc
     1741 atgcaatttg ccgtcgtcaa acatgggggc aagacgcatt tctatccgct cgtgcggctc
     1801 tccgaccttt tcaagcaatt cctcggaata ctcgctactg agcatttctt tggctttcct
     1861 gaaattagcc cacgcattcc tgagaatcgt atcagctgac attttttaac tcctgttcca
     1921 ataaatacct actttgcaac actgcaaagc ggtaaatata tagtattata atgaattagc
     1981 aaacaaattt aattaaaata atttattgct catataagtt aatcttatat ttttatgcct
     2041 tgcgagtcaa atgaatctct atggggttaa aaccgtagaa ttacaagaac ctatcccaaa
     2101 aacatgggcg tcgccgaaat cgagcaattt tgcaggcagc caagttttgg ggataggttc
     2161 taagggcata ttaaaaaggc tgaccttttc aggggtcagc cttgtgcaag acgttatata
     2221 ttataaaaag ttcttatcag aatttgtaat tcacgccgct actcagagcg ttactgtaat
     2281 cactgccggc cgcgaatccg tcgtaacgga ggaacaggga aaggtcgtcg ctaagcaatg
     2341 ccgtcacgct gacgccccat ataaagtggc ccttcgacgt actatccgta taaaacgtca
     2401 tcggataacc gccgacgaat ccggcatcca acgccttggg attcagataa tcgtatgaat
     2461 agccgaggct aaccgcagga acgagcttaa catacttcag gtcaaagagt ttgctgattt
     2521 caaccatgaa aatctggcgg aatgagtcgt tggtataatc gccgacctcc aacgcggctg
     2581 cgccgccggt ttcattagtg ccgttctggt tgtaatgcac gtactgcagg gccatgctgg
     2641 gcgttaaatc ccatccggct acgttgaatc tgtagcctac cctcgcataa ggcgtaacat
     2701 cccatgcgcc atagtcatcc gccgtatcaa catcggaaaa attatcatag ttgacctggc
     2761 gttccgcatc gttccagtga tagccgaaag tgagcgtgcc ttcaataaac agcttgccta
     2821 ccgaccagtc gatatatggg ccgacacgca atgtatcaac atccagttta cctcgcgaat
     2881 cattcatgtg atattcactg cgtatgtagc caatcaaggc gcctatccga aggcctggcg
     2941 caaattttct gtctgtaccg acaacagcgc cccatgcact attattatag ccggtgagtt
     3001 ccacagaggc cttttccttg tgccacatgt ttattatctg gccccacacg ctccattttg
     3061 tgctatcggt cgaatcaggg acacttaaag catattcacg tgcctcttct tcctgtttgc
     3121 cctcgataat ttcagccatc gtttcaggat ctatatctgt ggtagcaagt gacatgccca
     3181 ggctcgaatc cgcaacacgt gtaacctggc ggttattgcc attgcgcatc tgagtgagat
     3241 gctggctgat aaacctgttg aagctttcca tctgattagg cagattcgtc ccatgcatgg
     3301 tgtacatctc gccatccatc tgcttcaatg cgtaattgta gctgtctaca tcgtcaaagg
     3361 tcaaaagatt gttaagcatc gttttctggg cggcagtaac cttgtcctca taatcatcgt
     3421 catcttcatg ctggatatcg tcgagcgcgt cggcaagcat tgcatccaat gcgccagcgg
     3481 tggatttttc attctcgccg acagcagggc cggaaaagcc gctcgtctgg ctgagattca
     3541 ggcgatatac ctcatccgtc ccgctggtaa caatttcact attgaaatcg aaaaacagcg
     3601 atgaacactt tatactcgca ttatccgtaa ttgttccgcc agcagtaata acatcaaaat
     3661 aatcttcaag gaacagagac attatgctgc tgccgttgac actcacataa actgtcgaac
     3721 catcttccag aataacatcg ccgtccgtta ccgcaagagt atcactatta atggtcgagg
     3781 catccgtgtc aatgttgaca accagcgtag cgccgtcctt caaagtatat cctgtcgtcg
     3841 tagtatactc cgccacagac gctccttcag tccccatcgt gccatacatt ataagattgc
     3901 ctatgacgct aaaaccgcca tcgacagtca taagcggaat atactcatct tcatcttcgt
     3961 cgtaaatatc cgcgccgacg accacagctc cattaaccgc ggtaacgcta tcgccaatag
     4021 ccaccgcccc tgagccggat gtatcggtaa cgactatctg cgcattttca cccatctcca
     4081 aagtgccggc tgtcatggta gaattatcga gcacactgaa tccgctggtg gaatttacga
     4141 ccagcgcacc atcaatggca accgcggtat catctcctga gaccgtcaac gtggaagcgc
     4201 cattgccgct aaccgtaaaa tcgccgccga cattcatcgg ctgggaaatt gttgccgtgt
     4261 aataactgcc gctgaaggaa acgtcaccag tcacctcaat ggcagacgta ccgtcgaacg
     4321 tcaccgtact cattgatgaa ttgggataat tcgtatcatc actagtaacc gtcgtgttcg
     4381 aaatattcac agttatactg ccaaccgtaa tgtcacagtt cgtagcactg aaattcaacc
     4441 cgccatattt gctggttacg atatacaaac cgtatgactc attatcctca tcaatcacaa
     4501 gagtgctgtc ttcgagagtt acagagctgt cgacaaaatt cgtaccggtc aggtaaaggt
     4561 aatcggatat atttatcgta gaaccgttag tggcactcag cgtaccttta cccgtcgttc
     4621 cgatggcaag at
//
LOCUS       DAONFI010000184         4617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_22226_length_4617_cov_4.322008, whole genome shotgun sequence.
ACCESSION   DAONFI010000184 DAONFI010000000
VERSION     DAONFI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22226_length_4617_cov_4.322008"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1218)
                     /locus_tag="PLK08_06700"
     CDS             complement(328..1218)
                     /locus_tag="PLK08_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPS53223.1"
                     /translation="MTVIAGCACGGCSTEDNARRILEQDTSMGRLEAMTHDVDSMIKL
                     GTIDKHYVIKAADGINLDVWVIFSKKREYEKTKGTVMLLHGFEDSKRNMLSLGETLAG
                     DGYDVVLNDMRAHGRSGGKYVTFGAVERRDVLAIADYLLNEHVINQPLYAMGFSMGAA
                     TAILYAADDSRCKAVVAIAPYSDARTVLHHHSGYISDEQFAAAWARAGEIAGFQPDDA
                     DVIAAARKIRCPLLVIHGRLDAIVPYEQGKAVYEAAGGKKELKTVGIASHLSIIAMGD
                     KWFAEQLDRLTGNKQPKREK"
     gene            complement(1319..2299)
                     /locus_tag="PLK08_06705"
     CDS             complement(1319..2299)
                     /locus_tag="PLK08_06705"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPS53224.1"
                     /translation="MKAKALIIVTVAAAWVLAGCDENENRNLQVTTSSQNNIVMELAG
                     DGDKLVAQKKLEAAGEITSADGVKIAYWSIDAHGKSKGTAVILHGLYESKANYMGMGK
                     KLAADGYDVVLVDLRHHGDSGGKYITCGAKEKDDVKAIMDKLSAEGKIAQPFYVFGVN
                     YGAVTGILYAEIDPRVKGVVAIGPWQDTVTKFKRDLGLFASPEDLNKALENAKEDAGF
                     DPRGTSALESARHLTCPLYLIHGTADLIVPVSDSEAIFAVANKPKELKVVLAGSPEQI
                     IIGMDYDGWLARQVEKVAKDQVGKKKVSAKNKAAKAVKKTPAKKQAVEVK"
     gene            complement(2463..3713)
                     /gene="glyA"
                     /locus_tag="PLK08_06710"
     CDS             complement(2463..3713)
                     /gene="glyA"
                     /locus_tag="PLK08_06710"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010279033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPS53225.1"
                     /translation="MDRLAQEDPEIKALIEKEHDRQRNTIELIASENHVSIPVLQALG
                     SVLTDKYAEGYPEKRWYCGCEAVDEVEALAITRAKKLFNAEHANVQPHSGTNANLAVY
                     LAAINPGDKIMGMNLAHGGHLSHGLPINLSGKMYKTVFYGVESDSERLDMDKIRQQAK
                     TEKPAIIVAGASAYPRQIDFAAFGEIAREVGCLLMCDVAHIAGLVVTGQHPHPFPHAD
                     FITTTNHKTLRGPRGGLILCRQQWAKKIDTAIFPGIQGGPLMHAIAAKAVSFHEALQP
                     SFHEYIANVIKNARYFAGVMHEKGWRIVSGGTDNHLFLVDLRSKLPDVTGHDAANWLN
                     AAQIVCNKNAIPNDPRPPIKTSGLRIGTPAITTRGLGKDEINVLADLMDTVLMSNGDT
                     AVINRTAAKIHDLCKRFPLPNDKL"
     gene            3994..>4617
                     /locus_tag="PLK08_06715"
     CDS             3994..>4617
                     /locus_tag="PLK08_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPS53226.1"
                     /translation="MPHEQMNIKQLASYLSMDARELHKLASRQKLPCRKTGGNFIFTK
                     CEIDRWVWDTLNEFNSRELQGIERGVAKHHGFNADIPIICPLVHKNTMVMPLNVHSQD
                     AVLRGLVDSADAAGLIYDREHLLGELKAREQLCSTAILPGVALPHPRQPLPYDIAESF
                     IIPGLTPAGIPFGAEDGSLSRLFFLICCKDERTHLHVLARIVRMLDAP"
BASE COUNT         1039 a         1238 c         1138 g         1202 t
ORIGIN      
        1 tacgccgtta ttgccggcgc caactgctgg cgtctgagcc actgcattat ccatcgacac
       61 aaacaaaccc gtcagaagcg ctcccgccgc cataaacaga acagcgagaa ctcccagacg
      121 caaaataccc atctgtcgaa tctgtaaacc catagcgggc aattctctat ccaaatttta
      181 tcatttcagg cctattgaca catttgtcgc aatttattat ccgctgatta tagtgacggg
      241 aagtcggata atgcaagatt tttttcgatg cattgacatg ctttggcgaa aaaagatacc
      301 atctgacggc ctgtccgcca tatagattta tttttccctt ttcggctgtt tattgccggt
      361 cagccggtca agctgttcgg caaaccactt atcgcccatg gcgattatgc tcaggtgcga
      421 ggcaataccg acggttttaa gctccttttt gccgccagcc gcctcgtaaa ccgcctttcc
      481 ctgttcataa ggcactatgg cgtccaatct tccgtgtatg acaagcaggg gacatctgat
      541 ttttctggcg gcggcgatta catccgcgtc gtcaggctga aaaccggcga tttcacccgc
      601 acgagcccaa gccgccgcaa actgctcatc ggaaatatag ccggaatgat ggtggaggac
      661 tgttctggcg tcggagtagg gtgcgatggc aacgacggct ttacagcggg aatcgtcagc
      721 ggcatagagt atggcggtgg ctgcgcccat tgaaaaaccc atcgcgtaaa gcggctggtt
      781 aattacgtgt tcattcagaa gatagtcggc tatcgccagt acgtcccgcc gttccaccgc
      841 accaaacgtg acatatttgc cgccgcttct gccgtgcgct cgcatatcgt tcagaactac
      901 gtcataacca tcaccggcga gcgtttcacc aaggctgagc atattgcgct tggaatcttc
      961 aaacccgtgc agaagcatga ccgtaccctt ggtcttttca tattcgcgtt ttttgctgaa
     1021 aatcacccac acgtcaagat ttatgccatc ggcggctttt attacgtaat gtttatctat
     1081 cgtgccgagc ttaatcatgg agtcgacatc atgcgtcatc gcctcaagtc gccccatcga
     1141 cgtatcctgc tccagaattc gccgtgcatt atcttccgtc gaacagccgc cgcacgcgca
     1201 gcctgcgata actgtcatca aaatgaatcg caaaaaattt ctcattattc cttccccgat
     1261 tcatatgaaa cgggcctgtc aaatgacagg cccgaaaatc agctatcagc agcagccgtt
     1321 attttacttc tacagcctgc ttttttgccg gagttttttt tacagccttg gctgccttgt
     1381 ttttcgcgga aacttttttc ttgccgacct ggtctttagc aactttttca acctgcctgg
     1441 ctagccagcc gtcgtaatcc atgccaatga taatctgttc aggcgatccg gcgagaacga
     1501 ctttcaattc ttttggtttg ttggctacag cgaagattgc ttcgctgtct gaaaccggca
     1561 ctatcaaatc agccgttccg tgtatcaggt acagcgggca tgtgagatgt cttgcggatt
     1621 cgagcgccga tgtgccgcgc gggtcgaaac cggcgtcttc tttggcattt tcaagagcct
     1681 tgttgaggtc ttcggggctg gcgaacaggc cgaggtcgcg tttgaacttg gtaacggtgt
     1741 cctgccatgg gccaatcgca accacgcctt ttactcgcgg gtcgatttca gcataaagta
     1801 tgcctgtaac cgcgccatag ttcacgccga agacatagaa cggctgggcg attttgccct
     1861 cggcggagag cttgtccatt atagccttga cgtcgtcttt ctccttggcg ccgcaggtaa
     1921 tgtatttgcc gccgctgtcg ccgtgatgac ggaggtcgac aagaacaaca tcatagccgt
     1981 cggcggcaag ttttttgccc atgcccatgt agttggcctt gctctcgtaa aggccgtgaa
     2041 gaatgaccgc cgtgcctttc gatttgccgt gtgcgtctat ggaccagtaa gctattttaa
     2101 cgccatcggc tgaggtaatt tcaccggctg cttcgagttt tttctgagcc accagcttgt
     2161 cgccatcgcc agcaagttcc ataactatat tattctgtga agaagtggtg acctgcaaat
     2221 tgcgattttc attctcgtcg catcctgcca acacccatgc cgccgccacc gtaactataa
     2281 tcaacgcctt cgctttcatg ttgcagtctc cttgaaagaa gtcccttatg cacatgcaat
     2341 acgctgatat tgtgtcactc ctcagcccaa aatgcaagtg tctgatacaa aaaacgatgg
     2401 caaaaccgta tactgtcgcg atacggcgtc tatcgttgcg acgtacaagc agacgaacga
     2461 ttttacagtt tgtcgtttgg cagcgggaat cgtttacaaa ggtcgtgtat tttcgcggcg
     2521 gttctgttta tgacggcagt atcgccgttc gacatcagta ccgtatccat gaggtcagcc
     2581 aatacgttta tttcatcctt gcccaagccg cgagttgtga ttgccggcgt gccgatacga
     2641 agacctgacg tcttgatagg cggccgcggg tcgttgggaa tggcattttt gttgcagact
     2701 atctgtgccg cgttcagcca gttggctgca tcatggccgg taacgtcggg cagcttgctc
     2761 cgcaaatcca ccaggaacag gtgattatcc gtgccgccgg aaactatccg ccagcctttt
     2821 tcgtgcatta cgcctgcaaa atatcgggca tttttaatta catttgcgat atattcgtga
     2881 aaagacggct gaagcgcttc gtggaacgac accgcttttg cggcgatggc gtgcatgagc
     2941 gggccgccct gaatgccggg gaaaattgcc gtatcaatct ttttagccca ctgctgcctg
     3001 cacagaatca ggccgccacg agggccgcga agagttttgt gattcgtagt cgttatgaaa
     3061 tcagcgtgcg ggaacggatg aggatgctgg ccggtcacga caaggccggc gatgtgagcc
     3121 acatcgcaca tcagcagaca gccgacttcc ctggcgattt cgccgaaagc cgcaaaatct
     3181 atctgccgcg gatacgcact tgcgccggcg acgatgatag ccggcttttc cgtcttcgcc
     3241 tgctgccgga ttttatccat gtccagccgc tcactatcgc tctccacgcc gtaaaatacc
     3301 gtcttataca tcttgccgga cagatttatc ggcaggccgt gcgaaagatg gccgccgtga
     3361 gccaggttca tgcccatgat tttatcgccc gggttgatgg cagccaggta gaccgccagg
     3421 tttgcgtttg tgccgctgtg cggctgaacg ttggcgtgtt cggcgttgaa aagttttttg
     3481 gcgcgggtga tggcgagggc ttctacttcg tcgacggctt cacagccgca gtaccagcgc
     3541 ttttcaggat aaccttcggc gtatttgtcg gtaagaaccg accccaacgc ctgaagtacg
     3601 ggtatggaaa cgtgattttc cgacgctatc agttcaatcg tattgcgctg acggtcgtgt
     3661 tccttttcta tcaatgcctt tatttcaggg tcttcctgtg ccagtctgtc cattttcagc
     3721 ctttccttgc ttcattaatt caaaattgaa gacgcagagt ttaactctcc acccaatttt
     3781 tcgcaagcca ttaggcagga ttttcttaat caactgatgg aatatttgcc gaaaatggta
     3841 taataactac ccaaatactt tatccgaagg agttttacgg ttttagcgca agcctgctgg
     3901 gctgccgcta aagaaagatt caggcgtcag cctttttacg gggctgcaat gtgcccttga
     3961 aaaaccgtaa aatcatgaag gacttctcat aaaatgccgc acgaacagat gaatataaaa
     4021 cagctggcat catatctttc gatggatgcg agagaattgc ataagctcgc ttcacgccag
     4081 aagttaccct gccgcaaaac cggcggcaat ttcattttta ccaaatgtga aatcgaccgg
     4141 tgggtctggg acactttaaa tgaattcaac agccgtgaac ttcagggcat tgagcgcggc
     4201 gtcgccaaac atcacggctt caatgccgac ataccgataa tctgcccgct ggtacataaa
     4261 aacacgatgg tcatgccact caacgttcac tcgcaggacg ccgtcctgcg cggcttggtg
     4321 gattcagccg acgcagcagg cctgatttat gacagggagc atcttcttgg cgaattgaaa
     4381 gccagagagc agctttgcag cacagccatc ctgccgggcg tcgctttacc gcatccacga
     4441 cagcctctgc catacgatat tgcggaatct tttataatac cgggacttac gcctgccggc
     4501 ataccattcg gagcggaaga cggctcactg agcaggctgt tttttctgat atgctgcaag
     4561 gatgaacgga cgcatcttca cgtgctggcg cgtatcgtgc gaatgctcga tgcccca
//
LOCUS       DAONFI010000185         4574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_22506_length_4574_cov_3.768533, whole genome shotgun sequence.
ACCESSION   DAONFI010000185 DAONFI010000000
VERSION     DAONFI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22506_length_4574_cov_3.768533"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="fabF"
                     /locus_tag="PLK08_06720"
     CDS             <1..1068
                     /gene="fabF"
                     /locus_tag="PLK08_06720"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPS53227.1"
                     /translation="VMLAHEAKKLDWFAQFAVAASAEAVKDCGIDFDTIDRNRCGVIV
                     GSGIGGLQTLQEQHRRMLDKGPTKSSPFTVPRLMGNAAGAHIAILFGLGGPNFGCVTA
                     CASAAHSIGQAMRAIQNNEADLIITGGSEAALCEIGMSSFCALKGLSTRNDDPQHASR
                     PWDADRDGFLLAEGAGCITIEELEFAKARGARIYAELIGYGATCDASHITAPDPEGKG
                     AVRAMEICLADAGLAPAELDYINAHGTSTQLGDRAEITAIKKVFGDHATNGLLVSSTK
                     SLTGHLLGASGGVEIIACVKAIQEGIVPGTYNLNNPDEACEGMDLVPNAPRKKAIKTA
                     LSNSFGFGGHNGCLIIRKYEE"
     gene            complement(1171..3051)
                     /locus_tag="PLK08_06725"
     CDS             complement(1171..3051)
                     /locus_tag="PLK08_06725"
                     /inference="COORDINATES: protein motif:HMM:NF037720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPS53228.1"
                     /translation="MKFDAEFAWDRSQTPAELHRMLDAVGEYYPLFERQGGDGCRLSF
                     RRRENSGRLLVMRQGDSVTVEYDSVSGAARGVGLVMAGRDCDETAVFKTLGIMLDCSR
                     NAVMTVEHFKGWLLRLALMGYNMAMLYTEDTYELPDEPYFGYMRGPYTAEEIRELDDY
                     AKKLGIELVACIQVLGHMEQILQWAAYQPVKDTRNVLLVNEPKTYELIEKMIAFWSRT
                     LSTERIHIGMDETHDLGRGRFMDIYGYERGFDIFNRHLGRVGEICEKYGLRPMIWSDM
                     YFRLGNSRQDYYAPETVIPDDVKRQIPANVDLVYWDYYHREREFYSEWIKRHRELGHE
                     PMMASGIWTWGKLWCDHELTHATVQPCIEACRDSKLAEILFTMWGDDGAYCEFDSSLA
                     ELCRAADMAFGEKGTAADIEKSFGTICGGSYHVHQIASQLDYAFWENEKVQASLLLWD
                     DPLMMKYLREISKKLPDGYASIVRHYRSLAESLKPFENQTAGGCIALAFKAATFLADK
                     VELQNGLSTACSVRDIATVKDIAENGIPAIIGALRDFAAVFRRQWLNRNKRFGLDVMQ
                     IRFGGQIARWEEMAVTLNEFIAGKIATVPEFDAGKGVAESSAVPGCTWSRLAMSSSSL
                     LW"
     gene            3177..4025
                     /locus_tag="PLK08_06730"
     CDS             3177..4025
                     /locus_tag="PLK08_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPS53229.1"
                     /translation="MPKRNSKYEINYLPIPPNIAGILRQSGLMFYCYYDNVKSGIKDF
                     SRMRTFDYYCLLQFVDGNGYFKDDAHPVPVKIPREGGLIVIPGARQEYGAELDCFVED
                     SVCFYGPAADALFNAGIIRQGIIHFGKERRLLPIIEKIRTGLLSSYLEANVMLMQLLF
                     DLHWQNHAGKDFASAGNIEQLLQQINRKQQQMWTVKKMAKFCSISENHLRRLFRRHTG
                     MPPKQYLEQCKMRSATELLIGTDLKIAEIAAQLGYDDPYHFIRRFSTKIGISPGMYRK
                     QHLSRF"
BASE COUNT         1229 a         1257 c         1069 g         1019 t
ORIGIN      
        1 gtcatgctcg cccatgaagc aaaaaaactc gactggttcg ctcaattcgc cgtagccgcc
       61 agcgcagaag ccgtcaaaga ctgcggcata gatttcgata cgattgaccg taatcggtgc
      121 ggcgtcatag tcggcagcgg catcggcggg ctgcagactc ttcaggaaca gcacaggcga
      181 atgctggaca aggggccgac caaatcgagc ccgttcaccg tgccgcggct gatgggcaat
      241 gccgccggcg ctcacatagc catcctgttc ggcctcggcg gcccgaattt cggctgcgtg
      301 acggcgtgcg cgtcggctgc acactcaatc ggccaggcga tgagagccat ccagaacaac
      361 gaagccgacc tcatcataac cggcggcagc gaagccgctc tatgcgaaat cggcatgtcg
      421 agcttctgtg cactcaaagg tctgagcaca cgcaatgacg acccgcagca cgcatcgcgg
      481 ccgtgggacg cagaccgcga cggcttcctg ctggcagaag gcgcaggatg cattactatc
      541 gaagaactgg aattcgccaa agcccgcggc gcgagaatat acgccgaact tatcggttat
      601 ggcgccacct gcgacgccag ccatataacc gcacctgacc cggaaggcaa aggcgcggta
      661 cgtgcaatgg aaatctgcct cgccgacgca ggcctcgcac cggctgagct ggattacata
      721 aacgcccacg gcacaagcac gcagcttggt gacagggctg aaattacagc tataaagaag
      781 gttttcggcg atcacgccac aaacggcctg ctcgtctctt ccacaaaaag tctcaccggc
      841 catctgcttg gcgcatccgg cggcgtggaa atcatcgcat gcgtaaaagc catccaggaa
      901 ggcatagtac caggaacata taacctgaac aatcctgacg aggcgtgcga gggcatggat
      961 cttgttccaa atgccccgcg gaagaaggca atcaagactg cgctgtccaa cagtttcggc
     1021 ttcggcggcc acaatggatg cctgattatc cgcaaatatg aagaataacg attgcagctg
     1081 aaaaacaaag gcgatgcgtt aaagttgtta aaactattct aaaacgcatc gccttttttg
     1141 ttgtaaaaaa gaacatcctg catacgtaaa ttaccacaac agcgaagatg aactcatcgc
     1201 cagtctgctc cacgtacagc cgggtactgc cgacgactcc gccacacctt tgccagcgtc
     1261 gaactcaggg acagtcgcaa ttttgccggc gatgaactca tttagcgtta ccgccatctc
     1321 ttcccagcgt gcaatttgtc cgccaaatct gatttgcata acgtcaaggc caaacctctt
     1381 gttgcggttc agccactgcc tgcggaaaac tgcggcaaaa tcgcgtaatg ccccaataat
     1441 cgccggaatc ccattttctg cgatatcttt gacggttgca atatcgcgta cagagcaggc
     1501 tgtcgataat ccgttctgaa gttcaacctt gtcggctaaa aacgtcgcag ccttgaatgc
     1561 cagggcgatg cagccgccgg cggtttgatt ttcaaacggc ttgagacttt cggccaggct
     1621 gcgataatgc ctgacaatcg atgcatatcc atccggaagt tttttggaga tttcccgtag
     1681 atatttcatc ataagcgggt cgtcccacag cagcagagat gcctgtacct tctcattctc
     1741 ccaaaaagcg taatcaagct gcgacgcaat ttgatgaaca tggtaacttc cgccacatat
     1801 cgtcccaaaa ctcttctcga tgtcggcggc cgttcccttt tcgccgaatg ccatatcagc
     1861 cgccctgcac agctcagcca gcgatgaatc gaattcgcaa tatgccccgt cgtcacccca
     1921 catggtgaac agaatctcgg cgagttttga gtcgcggcat gcctcgatgc acggctgaac
     1981 ggtggcatgc gtaagctcgt ggtcacacca gagcttgccc catgtccaaa ttccggaggc
     2041 catcataggc tcatggccca attcacgatg ccgcttaatc cattccgaat aaaactcgcg
     2101 ttcgcgatga tagtaatccc agtaaaccaa atccacgttc gccggaatct gccgctttac
     2161 gtcgtcggga atgacggttt caggggcata ataatcctgc ctggagttac ccagccgaaa
     2221 atacatgtcc gaccatatca taggcctcaa accatacttt tcgcatatct cgccaaccct
     2281 gccgagatga cgattgaaaa tgtcaaatcc cctctcataa ccgtaaatat ccatgaaccg
     2341 gccgcggccg agatcatgcg tctcgtccat gccgatatga atccgttcag tcgacagagt
     2401 ccggctccag aaggctatca tcttttcgat gagttcatat gttttgggtt cattgaccag
     2461 cagtacatta cgggtgtctt ttactggctg ataggcggcc cactgcaata tctgttccat
     2521 atggccgagc acctggatgc aggcgacaag ctctatgccc aattttttcg cataatcgtc
     2581 caattcgcgg atttcttcgg cagtataggg ccctcgcatg tatccaaaat acggctcgtc
     2641 gggcaattcg tatgtgtcct ccgtatacag catggccata ttgtaaccca tcaacgcaag
     2701 ccgcagcagc catcctttga aatgctcgac cgtcatcacc gcattcctgg aacagtcaag
     2761 cattatgccc agagttttaa aaacggcagt ctcatcgcaa tcgcggccag ccatcaccag
     2821 cccgacgccc ctggctgcac cgctcacgct atcgtactca acagtcacac tgtcgccctg
     2881 ccgcatcacc agaagacgtc ctgaattttc acggcggcgg aacgacagcc ggcagccgtc
     2941 gcccccctgt cgctcgaaca acggataata ttccccgacg gcatcaagca tccgatgcaa
     3001 ttctgcggga gtctgtgacc tgtcccatgc gaactcggcg tcgaatttca ttttaaaacc
     3061 tttcaataat ggataaaacg attatcttat ttaaacaata aaaacctttt ttgtctgcaa
     3121 ttaatagtta taatcgatgt cgtaaaatat ggaaatggat atttcaacca taaaaaatgc
     3181 caaaacgaaa cagcaaatac gaaataaact acctcccgat tccaccgaat atagcaggca
     3241 tacttcggca gagcgggctg atgttctact gctattacga caatgtaaag tcaggcataa
     3301 aagatttctc ccgcatgaga acttttgatt attactgtct gctgcaattc gtcgacggca
     3361 acggttattt caaagacgac gcccatccag tgcccgtgaa aattccgcgg gaaggcgggc
     3421 tgatagtaat ccctggagca cgacaggaat acggcgctga gcttgattgc ttcgtcgagg
     3481 actccgtttg cttctacggc ccagccgccg acgcactttt caacgcgggc attatccgcc
     3541 agggaataat ccacttcggg aaagaacggc gactgctgcc gattatcgaa aaaatcagaa
     3601 caggccttct cagcagttat ctcgaagcga atgtcatgct catgcagctg ctgttcgacc
     3661 tgcactggca aaatcacgca ggcaaagatt tcgcatcagc cggcaatatc gaacagctgc
     3721 ttcaacagat aaatcgcaaa caacaacaga tgtggactgt aaaaaaaatg gccaagtttt
     3781 gcagcataag cgaaaatcat ctgcgaaggc ttttccgccg acacacgggc atgccaccca
     3841 aacaatacct cgaacagtgc aaaatgcggt cagccaccga actgcttatc ggcacagacc
     3901 tgaaaatagc ggaaatcgcg gctcaactgg gctacgacga cccgtaccat tttatacggc
     3961 gattcagtac gaaaatcggc atctcgccag gaatgtacag aaaacaacat ctatcgcgat
     4021 tctgaaaagc tgcaagttgc atcccactgt ttactgaaac ttattgtcgc tcaggatgaa
     4081 tatctacggc aactggcgac attttttacc cacactgcga cagaattgca cttttcagcc
     4141 caagtgacca acaaaaaaag taaaaaccct tgaccaatgc cgcatgattg acgtattata
     4201 ggcactcata atccggttct gctccgtaaa attttgacag taatatgaca aaagtcagtc
     4261 cggcagaaac ctgaaactgg aaaatttaaa gccatcataa taatcatatt tgtaatatct
     4321 cttttccagg ccaggataat gcggcggctg tctgcggggt tgacgaccgt cgacattccc
     4381 aatcggccag tccatattaa cgattatcag tgtatacagc atagctgaaa tgcagtcagc
     4441 gtctttatat acttcatatt gttctttttg cgagtaagtt ggatatggca cgaaaaaaca
     4501 agaaactagg cgaaattttg tgtgattggg gggcagttac gaaggcaggt ctggaggagg
     4561 cattggaata cgga
//
LOCUS       DAONFI010000186         4525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_22857_length_4525_cov_4.097539, whole genome shotgun sequence.
ACCESSION   DAONFI010000186 DAONFI010000000
VERSION     DAONFI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22857_length_4525_cov_4.097539"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            723..1076
                     /locus_tag="PLK08_06735"
     CDS             723..1076
                     /locus_tag="PLK08_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPS53230.1"
                     /translation="MNLSDLKNDFSRFHRKPAAGMNAASRRRPPKLYRVGEVVEYSGV
                     SRQTIHNYTTMGLIHTTGWTRGGHRLYDEGVFSRLDMIAEFKRGNRSMDFIRTFFEKM
                     ENATAAEHIAAGLIE"
     gene            1126..1914
                     /locus_tag="PLK08_06740"
     CDS             1126..1914
                     /locus_tag="PLK08_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPS53231.1"
                     /translation="MKKTRKSTMEEVQQWWNSFKKCGDDDSRNKLLEHYLPIVRYTAD
                     RLRTKLPEEVDVDDLVSSGIFGLLDAINAFDVKRGVKFETYCAQRIRGAILDELRALD
                     WVPRLVRSRTHKVNEASGSLEAQLGRKPEHAELAKALKISQKEFTRLCRDSEAVNVVS
                     LSTKWFETDSNKDVTKADMIEDKNSDNPLEMSQRRDLKELLTRGLSRAERLIVILYYF
                     EEMTMKEIGETLDLSESRVSQMHSAIMERMREQLAVRKMDLQYV"
     gene            complement(1979..2785)
                     /locus_tag="PLK08_06745"
     CDS             complement(1979..2785)
                     /locus_tag="PLK08_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPS53232.1"
                     /translation="MFKTILARLQQGHRTMKYPDGPAPALSDRFRGRPAVDANICQNG
                     CRLCLEACPTDAIKISDNEVNIDLGRCLFCGECAKACPAGAIRLTGDYRLAVRNREDL
                     KITANRQTIKLAAALEGKMKKLFGRSLKLRQVSAGGCNACEADTNVLNTLAWDLGRFG
                     IQFVASPRHADGLLITGPVTQNMKLALKKTYDAVGEPKIVIVVGTCAISGGPYIGHEQ
                     VNDGIADIVPVDLYIPGCPPHPLTILDGLLRLLDRKTPELFDNSDKVRQS"
     gene            complement(2833..4365)
                     /locus_tag="PLK08_06750"
     CDS             complement(2833..4365)
                     /locus_tag="PLK08_06750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPS53233.1"
                     /translation="MNSENMNFVTLTNGRAANLESIPTLEFKDFSRAVIDKVATGGRV
                     AAMFGAASHQPAVTHIYAIIADDSQNRLCVTRTSVEGCEFESLTPQCPQVHMFEREIA
                     EQFGLKPIGHPWLKPVRYHRSWTNRDAWGRSAGKAIQPAVCDFYRVEGDEVHEVAVGP
                     VHAGVIEPGHFRFQCHGEKVLHLEIALGYQHRGVEHAIAGGPDKRSIHYAETLAGDTT
                     VAHSTAYCQAVEALSRCQKTAHAQMLRGVALELERLANHIGDLGAMAGDVGFLPTSSF
                     CGRIRGDVLNMTALLCGSRFGRGMIRPGGVGFNINDMLAEELLKRLTAVEKDTTIAIN
                     MLWDTSSVMARFEDTGSLPKNIAVELGVVGPAARAAGLTQDVRQDQPSGIFRFIHVPV
                     STYDTGDVFARAFVRWLEIQHSFEFIREQLDTLPAGEISVPLNPLTPNSIAVTLGEAW
                     RGEVCHAAITDEHGRFARYKVVDPSFHNWPALAYVLRNQEISDFPLCNKSFNLSYCGN
                     DL"
BASE COUNT         1104 a         1076 c         1196 g         1149 t
ORIGIN      
        1 gcccgcaaaa ttgctcgatt tcggcgacgc ccatgttttt gggataggtt ctaataatgg
       61 tagggcagtt tctctaatta tgagataaat tttatgattc atgttgcaga ggcctgcttt
      121 gggaatttta tgataaaaat atgcgtctga tgtgtataaa aaagaatctg atagcggagc
      181 atttcttttt cggttcgacg gcgattacca tattttgaat atttgtgaga tttttaaata
      241 acacgtaatg agatgctaaa gcaattcctg aaaataatgc acacaagata gaatatatag
      301 ttcttttgcg aagggtagag cctaaggttt gataaaaaaa tgctttgtgt gggttatttt
      361 acgttgaacg cgatgttcag tttcgccttc cttgccagcc ttgtcttcaa cgacgcagca
      421 aagcgcaatt tcaaatttaa catgctctaa atataaaaat cgcaattgtg tatatcgggc
      481 tttgtgggga aaagataaaa aaattttcta aaaaatttat ttttatattt tacaaaacgt
      541 aaaaataaaa tagaatcgct ttcagaaaag gcagagttcc tgcgaagtat ggattcgcag
      601 gcgaattatc ggattacaag tgcttgtcgg ataggatggt tgcaagcgag gtgttcgctg
      661 aagcgggctt tgccggattt gtgagaaagt gaatcggata ctttataaat ttggcgagcc
      721 aaatgaattt atcggacctt aaaaacgact tcagcagatt tcatcgcaag ccggctgccg
      781 gaatgaatgc ggcatctcgt cgcaggccgc cgaaactgta cagagtcggc gaggtcgtcg
      841 aatattccgg cgtaagccgc caaaccatcc acaactacac caccatgggc cttatccata
      901 ctacaggttg gactcgtggc ggccatcgcc tgtacgacga aggcgttttt tcaaggctgg
      961 acatgattgc ggagttcaaa cgcggcaaca ggtcgatgga ttttattcgg acgttttttg
     1021 aaaaaatgga aaatgcgact gcagcggaac atattgccgc cggtcttatt gagtgaggct
     1081 gaccaagcca gggacggctg gtcaatagta caaggagaca ctgcgatgaa aaagacacgg
     1141 aagtctacga tggaagaggt tcagcagtgg tggaattcat ttaaaaaatg cggcgatgac
     1201 gactcccgca acaaattgct tgaacactat ctgccgattg tgcgatatac agccgacagg
     1261 cttcgcacaa aattgcctga ggaagtggac gttgacgatc ttgtgtcttc gggcattttt
     1321 ggtctccttg acgccataaa tgctttcgac gtcaaacgcg gtgtcaaatt tgaaacatat
     1381 tgcgctcagc gaatccgtgg tgcgatactg gacgaattgc gggcgttgga ctgggtgccg
     1441 cggctggttc gcagtcgcac tcacaaggta aatgaagcgt cgggctcgct tgaagctcag
     1501 ctcggcagaa agccggagca cgcagagctt gccaaggctc tcaaaatatc gcaaaaagaa
     1561 tttacacgtc tgtgcaggga ttcagaggca gtaaacgtcg tttcgttgag tacgaagtgg
     1621 tttgagacgg attcaaacaa ggacgttacc aaggctgaca tgattgagga taaaaacagc
     1681 gacaacccgc tggaaatgag tcagcggcgt gatctcaagg agcttctcac cagaggcctc
     1741 agcagggcag agcggctgat agtcattctt tattattttg aagaaatgac catgaaggaa
     1801 atcggcgaga cgctggatct ttccgaaagt cgggtcagcc agatgcattc cgcaattatg
     1861 gaacgtatgc gagaacaact tgcggttcgg aaaatggatt tgcagtatgt ctaacattga
     1921 atactgccaa tagccggcta atactgcatt ttgtaaccga tttgatttac ctgcaatttc
     1981 aagattgcct gactttgtcg gaattgtcaa agagttcagg cgtttttctg tctaataatc
     2041 tcaataagcc gtcaagaatc gtcagcggat gcggcgggca gcctggaata tataaatcga
     2101 caggcacaat atccgcaatg ccgtcgttta cctgttcatg tcctatgtaa gggccgccgg
     2161 aaatggcgca tgtgccgact actattacaa ttttaggttc gccgacggca tcgtaagttt
     2221 ttttcaacgc gagtttcata ttctgcgtta cagggccggt tatcagcagg ccgtcggcat
     2281 gtcgcggcga cgctacgaac tgaatgccga aacgtcccag gtcccatgcc agtgtattga
     2341 gcacattggt gtcggcttca caggcattac aaccgccggc acttacctgg cgcaatttga
     2401 gcgaccggcc gaaaagtttt ttcatctttc cttccaacgc tgccgccagt tttattgtct
     2461 gtcggttagc agttattttc aggtcttcac gatttcgtac tgcaaggcgg taatctcctg
     2521 taagacgaat agcaccggca gggcaggcct ttgcgcattc gccgcagaag agacatcttc
     2581 cgaggtcgat attgacttca ttgtcgctga ttttaattgc gtcggtaggg catgcctcaa
     2641 ggcagaggcg gcagccgttc tggcatatgt tggcatcgac ggctggacgg ccgcgaaatc
     2701 tgtccgacaa tgccggcgca ggcccgtcgg gatatttcat ggttcggtgg ccttgctgca
     2761 accttgctaa aatcgtcttg aacatagttg ttgattcctc gtcgaaattc ctgctacgct
     2821 gatttcgtat ctttacaggt cattgccgca atatgacaga ttgaaacttt tgttacatag
     2881 cgggaaatct gagatttcct ggtttcgcaa aacatatgcg agcgccggcc agttatggaa
     2941 cgacgggtcg actaccttgt aacgggcgaa tctgccatgt tcgtcggtta tcgccgcgtg
     3001 gcagacttct ccgcgccatg cttcgccgag cgtgacggca atgctgtttg gcgtgagcgg
     3061 gttgagcggc acggaaattt cgccagccgg cagagtgtcg agctgttcgc gtatgaattc
     3121 aaaagaatgc tgtatttcca gccatcgtac gaatgcgcgg gcgaaaacgt cgccggtgtc
     3181 atatgtcgag accggcacat ggatgaaacg aaatatgccg gacggctgat cctgtctgac
     3241 gtcttgcgtc agtccggcgg cccgtgcggc tggccctacg acgccgagtt cgacggcgat
     3301 gtttttcggc agcgaaccgg tatcctcaaa cctggccatc acggaggacg tgtcccacaa
     3361 catatttatc gctattgtag tatctttttc aacggccgtc agccgtttga gaagctcttc
     3421 cgccagcatg tcgttgatat tgaaaccgac accgcctggc cgaatcatgc ccctgccgaa
     3481 ccggctgccg cagagcagtg ccgtcatgtt cagtacatcg ccgcgtatac gtccacaaaa
     3541 actcgacgtc ggcagaaaac ccacatcgcc agccatcgcg ccgagatcgc ctatatggtt
     3601 tgccagccgc tccagttcca gcgccacgcc tcgcagcatt tgagcgtggg cggtcttctg
     3661 gcagcgggag agggcttcta cggcctgaca gtatgccgtc gagtgtgcta ctgtagtgtc
     3721 gccggctaac gtttcggcgt aatgaatcga ccgtttgtcc ggcccgccgg ctatggcgtg
     3781 ctcaactccg cgatgctggt aaccaagcgc tatttcgaga tgtaaaactt tttcgccgtg
     3841 gcactggaag cggaaatggc cgggttctat gacgccggcg tgtacagggc cgacggccac
     3901 ttcatgtacc tcgtcgcctt caacacgata aaagtcgcag acggctggct gaatggcttt
     3961 gccggcactg cggccccacg catcccgatt cgtccacgac ctgtgatatc gtaccggttt
     4021 cagccacgga tgcccgatag gtttgaggcc gaattgctcg gcgatttcgc gttcgaacat
     4081 gtgcacctgg ggacactgcg gcgtaagcga ttcgaactcg cagccctcta cggacgttcg
     4141 cgttacacaa agacgattct gactatcgtc ggcgattatt gcataaatat gcgttacagc
     4201 cggctgatgc gacgctgcgc cgaacatcgc cgcaacgcga ccgccagtgg cgactttgtc
     4261 tatgacggct cgcgagaagt ccttgaattc aagggttggt atactctcaa ggttggcggc
     4321 tcggccgttt gtcagcgtta caaaattcat gttttcgctg ttcatgccat gccctccaga
     4381 aaacgtactg cgtcgtttaa catgttttcc agcgggtttg gtatgtacaa acctagcaga
     4441 agaacggcgg ccatgagtat cacggctggt gctgtcgtca gaaaatcttc actgtacggc
     4501 tcgttcagga gttcttttgg aacac
//
LOCUS       DAONFI010000187         4522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_22881_length_4522_cov_4.397358, whole genome shotgun sequence.
ACCESSION   DAONFI010000187 DAONFI010000000
VERSION     DAONFI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22881_length_4522_cov_4.397358"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..755)
                     /gene="recR"
                     /locus_tag="PLK08_06755"
     CDS             complement(156..755)
                     /gene="recR"
                     /locus_tag="PLK08_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPS53234.1"
                     /translation="MSYSRTIETLIAELGKLPGIGARTAERLTFHLLKAPDEEALALA
                     RAIHDIKTLIHPCKVCFNPTEGDLCSICSDESRDAGVICVVEQPKDLLSIESSGAYNG
                     RYHVLMGAIAPLDGVEAEDLTISQLVDRVQAGGVREVILATNPTVTGDSTSLYISSLL
                     EKFPNVRITRLARGLPAGGQIEYASKTILSDAIKDRKNL"
     gene            complement(895..1236)
                     /locus_tag="PLK08_06760"
     CDS             complement(895..1236)
                     /locus_tag="PLK08_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPS53235.1"
                     /translation="MFGDLGSLGRMMQIAGQLKTKLPEMLEQLDAAEFVGQAGDGAVT
                     ATVNGKMKLKDLKLSADLKKDGQLDLEMIEDLVKAAVSSAQTQAADAMKAQFRELTGG
                     VDLGGMGKLMG"
     gene            1441..2793
                     /gene="rimO"
                     /locus_tag="PLK08_06765"
     CDS             1441..2793
                     /gene="rimO"
                     /locus_tag="PLK08_06765"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPS53236.1"
                     /translation="MKKIGIISLGCPKNLVDSEKMLAELAEAGCLLPAPLEEADVILI
                     NTCGFLAASRQEALDAIGQALELKHAGSARRVVVAGCLPSRFGPGCIPAGVDAIIGVN
                     DRDKIVQAVCGEGEFVSVCKEAAGFGPGESAGDAGRFRLTPPHTAYLRIAEGCDRKCT
                     FCTIPAIRGPMRSKPLDMVLAEARELIDSGAIELNIIAQDTTAWGEDLGDEWNLSLLL
                     ENLDRLDGAGWLRLMYTYPRNFGDELIEAIATAEHVVPYVDMPLQHISSSVLKRMGRR
                     IDRPATEELLHKLRERIDGLVLRTSLIVGFPGESDEEYGELLDFVKKFRFDNLGVFEF
                     SPEEGTPAAKLPGQLPDDVVADRADEIMAVQHEIVLENNRAMVGRKVDVLIDGEADDQ
                     ACTFGRYYGQAPDIDGLCLLNEPAEPGRILSCTVTGVDDYDLQVTPSSRRQKGCKVKG
                     "
     gene            2844..3965
                     /locus_tag="PLK08_06770"
     CDS             2844..3965
                     /locus_tag="PLK08_06770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPS53237.1"
                     /translation="MQNVPLSSQDITEAEITAVVNVLKSDRLSIGPRIEAFEASCAKA
                     GGRKYGIAVNSGTSGLHLCVKSLGLGEGDEVITTPFSFVSTTNCLLFERATPVFVDID
                     PVSYNVDPAAIEAAITPRTKAILPVEVFGNTAHFDEYERIAEKHGLKFIEDCCEALGA
                     VLDGRNAGSFGDCGVFAFYPNKQITTGEGGMIVTDDEEIRDLCRSYRNQGRATAAWLS
                     HARLGYNYRISELTAALGEVQASRLDEILGKRRRAAEMYTEELASLVGDIHLPPMVKI
                     ELPSWFVYVIRLDDRYPADARDKVMDFLKQHGIGCNNYFVPIHYQPYIRKMLGTKPGD
                     FPVTERIAARTIALPFFSNLSHEQVCRVRDVLKDALASL"
     gene            3965..>4522
                     /locus_tag="PLK08_06775"
     CDS             3965..>4522
                     /locus_tag="PLK08_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53238.1"
                     /translation="MNPIMRTYWTAKGVGWDNLPRRILQLLRVRSGWLAAKLKPENFT
                     DSAYAGELLKPVDWRNVASRMPFKIPSRETLGKIVTDHLWRFRVERIAEKAHSREFMY
                     FSNWFAKPRDFNHDPVNDVHWPVGEHWLRGDTLGGTDIKLLWEPNRFSVGYFLGRHYV
                     RSGDESVAELFWRLVDDWVEQNPPEL"
BASE COUNT         1036 a         1151 c         1247 g         1088 t
ORIGIN      
        1 gcctgacgca gccagccgac gccgcaaaca gcaacaagcg caaagtcaag tttaacatgc
       61 tctaattatt cggatgactt tgctgtggga ttttttaaaa aaacggtgaa gttgcgatgg
      121 ccggcacatg gccggtcaga ctgtttccga aatttttaga ggttttttct gtccttgata
      181 gcgtcagaca gtatggtttt gctggcatat tcgatttgtc cgccggctgg caggcctctg
      241 gccagccgtg ttatgcggac gttgggaaat ttttccagca gcgacgatat ataaagtgac
      301 gtcgaatcgc ctgtgaccgt cgggtttgtc gccagtatta cctcacgtac tccaccagcc
      361 tgcacgcggt ccaccaactg tgatattgtg agatcttccg cctcgacgcc gtccagaggc
      421 gctatggccc ccataagcac gtgatatctg ccgttatatg cgccggacga ttctatgctc
      481 aacaaatcct tgggctgttc caccacgcag atgacgccgg cgtcgcgaga ttcgtccgag
      541 cagatgctgc acaggtcgcc ctcggtgggg ttgaagcaga ccttgcatgg atgaataagc
      601 gttttgatgt catgtattgc gcgggctaat gccagagcct cttcatcggg cgctttcaag
      661 aggtgaaacg tcagccgttc cgccgtcctt gcgcctatgc cgggcaattt gccgagttcg
      721 gctatcagtg tctcgattgt gcgggaataa gacatctttt ctttatatgc ccttacaaaa
      781 ttccgctata gagcgatatt cgccagcctg aattggaaac acccaaaaaa ggtttgtttt
      841 aaagcctgta aatttttgcg gattaattgc ggtaaatttc ataaacaaga tttcttaacc
      901 catcaatttt cccataccgc cgaggtcgac tccgcctgta agttcacgga attgggcttt
      961 catggcatcg gcggcttggg tctgggctga agagacggca gctttgacga ggtcttctat
     1021 catttccagg tcaagttggc cgtctttctt caggtcggct gaaagtttca gatccttgag
     1081 tttcattttt ccgttgacgg tggcggttac cgctccgtcg ccggcttggc cgacgaattc
     1141 tgcggcatca agctgttcga gcatttcagg cagcttcgtt ttgagctggc cggctatctg
     1201 catcattctg cctaggcttc cgaggtctcc aaacatatat gtctcccgaa aagaaggtaa
     1261 atttaacccg aaactatatt ttactaaatt ttactaaaaa ctgcatatct gtcgcggctg
     1321 gctttttacg attagccctg ccgcgaaagc agattctaaa acgacatctc gtgcaaaaga
     1381 atttatccgg acgaacggaa tacttcaagc attggatgaa atttttgtta ttattctgtc
     1441 atgaaaaaga taggaataat ttcgctgggt tgtcccaaaa atctcgttga cagtgagaaa
     1501 atgttggcgg aattggcgga ggcaggctgt ctgctgccgg ctccgctgga ggaagcagac
     1561 gttatattga taaatacgtg cggttttctt gccgccagcc gccaggaagc attggacgct
     1621 atcggccagg cgttggaact gaaacatgcc ggcagcgcaa gacgtgttgt ggtagcgggt
     1681 tgcctgccgt cgcgattcgg gcctggctgc ataccagccg gcgtggacgc cataatcggc
     1741 gtaaatgacc gcgataaaat agtgcaggct gtttgcggcg aaggcgaatt tgtcagcgtc
     1801 tgcaaggaag ctgccggttt cggccctggc gaatcagccg gcgacgcagg ccgttttcgg
     1861 ctgaccccgc cgcataccgc ctatcttcga atcgccgaag gttgtgacag aaaatgcact
     1921 ttttgcacga ttccagccat tcgcgggccg atgcgttcca aaccgctcga tatggtgctg
     1981 gctgaggcga gagagcttat agattccggc gcaatagagt tgaatatcat tgcgcaggat
     2041 accacggcct ggggtgaaga cctcggcgat gaatggaatc tttcgttatt attggaaaat
     2101 ttggacaggc ttgacggcgc aggctggctg cggctgatgt atacatatcc gcggaatttt
     2161 ggcgacgaac tcatcgaggc tatcgcgact gctgaacatg tcgttccgta tgtcgatatg
     2221 ccgttgcagc atatttcatc gtcggttctc aaacgcatgg gccgacgcat agaccgcccg
     2281 gctaccgaag aactgcttca taaactgcgg gaacgcattg acgggttggt gttgcgaacg
     2341 agtttgattg tgggttttcc tggcgagagc gatgaagaat acggcgaatt gctggatttc
     2401 gtcaaaaaat tcaggtttga caacctgggc gtgtttgaat tttcgccgga ggaaggtacg
     2461 ccggctgcta aactgcccgg ccaactgccc gacgatgtgg tggctgaccg tgccgatgaa
     2521 atcatggctg tccaacacga aatcgtgctg gaaaacaaca gggctatggt cggccggaaa
     2581 gtagatgttc ttatcgacgg cgaggcggac gaccaggcct gcacctttgg ccgatattac
     2641 ggacaggctc cggatatcga cggtttatgc ctgttgaatg aaccggctga accgggccgg
     2701 attctctcgt gcaccgtcac cggcgtcgat gattacgact tgcaggtgac gccgtcaagc
     2761 cgccggcaga agggctgcaa ggtaaaaggt tgaggattgt gctggtttct gatataatca
     2821 cctctcattt tttggagaga atcatgcaga atgtgccttt gtcttcgcag gatataactg
     2881 aagcggaaat aaccgccgta gtcaacgtgc tcaagagcga ccgcctgagc atcgggccgc
     2941 gtattgaggc gtttgaagcc tcctgtgcta aagccggcgg cagaaaatac ggcatagccg
     3001 tcaatagcgg cacaagcggc ctgcacctgt gcgtaaagtc gctgggcctc ggcgaaggcg
     3061 acgaagtcat aaccacgccg ttttcgttcg tctcaaccac aaactgcctg cttttcgaac
     3121 gggctacgcc ggttttcgtt gatatcgacc ctgtgagtta taacgtcgac cctgccgcga
     3181 ttgaggctgc gataacgccg cgaacgaagg cgattctgcc ggtggaggtt ttcggcaata
     3241 ctgcgcattt tgacgaatat gaacgcattg ccgaaaagca cggcctgaaa tttatcgaag
     3301 actgctgcga agcgctgggc gccgtgctcg acggccgaaa tgccggcagt ttcggcgatt
     3361 gcggcgtatt cgcgttttac cccaacaagc agataaccac cggcgaaggc ggcatgattg
     3421 tgacggatga cgaagaaatc cgcgacctct gccgcagtta tcgcaatcag ggccgcgcca
     3481 cggcggcatg gcttagccac gcccgactgg gttataatta tcgcataagc gaattgacgg
     3541 cagcattggg cgaagtgcag gctagccgcc tggatgaaat tctcggcaaa cgccgccggg
     3601 ccgctgaaat gtataccgaa gaactcgcct cgctagtcgg cgatattcat ctgccgccga
     3661 tggtaaaaat tgaactgccc agctggttcg tttacgtgat acgactggat gaccgctatc
     3721 ccgccgacgc acgcgacaaa gtcatggact tcctcaagca gcacgggata ggctgcaata
     3781 attattttgt gccgattcac tatcagccgt atatccgcaa aatgctcggc acgaaaccag
     3841 gcgacttccc cgtaaccgag cgcatcgccg cacgaacaat cgctctgccg ttcttttcaa
     3901 acctgtcgca cgaacaggtc tgccgcgttc gcgacgtatt gaaggacgca ctggcatcgc
     3961 tttgatgaat cctataatgc gtacatactg gacagccaaa ggggtcggct gggacaatct
     4021 gcctcgccgg attcttcagt tattgcgcgt aaggagcggt tggctggcgg ctaaactcaa
     4081 gcctgaaaat tttaccgata gcgcttacgc cggcgaactt ttaaaacccg tcgactggcg
     4141 aaatgtcgcc agccgaatgc cgttcaaaat accatcgcgg gaaacgttgg gaaaaatcgt
     4201 gacagaccac ctctggcgat ttcgcgtcga acgtatcgct gaaaaagccc atagccgcga
     4261 gttcatgtat ttcagcaact ggtttgcaaa acctcgcgat ttcaaccatg accccgtcaa
     4321 tgacgtgcat tggcctgtcg gcgaacactg gctcaggggc gatacgctcg gcggaacgga
     4381 cataaaactg ttgtgggagc cgaaccgttt ttccgttgga tattttctcg gccgacatta
     4441 cgtccgcagc ggcgacgaat ccgtcgccga gcttttctgg cggctggtgg atgattgggt
     4501 ggagcagaat ccgcctgagc tt
//
LOCUS       DAONFI010000188         4472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_23230_length_4472_cov_3.600634, whole genome shotgun sequence.
ACCESSION   DAONFI010000188 DAONFI010000000
VERSION     DAONFI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23230_length_4472_cov_3.600634"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /gene="truD"
                     /locus_tag="PLK08_06780"
     CDS             <1..454
                     /gene="truD"
                     /locus_tag="PLK08_06780"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HPS53239.1"
                     /translation="FQSAVFNDILAMRIDGIDTLLAGDMACKSDTGGIFYVENPADEQ
                     PRADRYEISPTGMVPGYRSNFAVGRQGEIEHEVLSRRGVELDMFKNLGPLKAKGTRRI
                     LRYCLHEPDIDAGSDGNGTFIEVRFCAPAGCYATSLLREMMKTEIEGE"
     gene            456..1277
                     /locus_tag="PLK08_06785"
     CDS             456..1277
                     /locus_tag="PLK08_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPS53240.1"
                     /translation="MYIKGIDIHSHAFPDAIAHRAMAAIQSRCPDWPARGDGTIAGLA
                     ESMRKNNIEKSAVCSIATKPGQAEGICRWLAEVVAAHDCFIPVASVNPDDSNPRDWMK
                     MFRRDGIGIVKLHPMYQNFVVDDEKMYPIYDAVAEYDMLLEFHSGMDIGFPDDAVPDR
                     AAPHRFSKIARKFPQLKMLLAHMGGFLIWDDVEKELVGCNNIWFETSFGLTHMSREQF
                     VRMVRNHGEDKICFGSDWPWIDQGELWEKVLECDLGSEANAKIWRHNAAKLLGVG"
     gene            1331..3919
                     /locus_tag="PLK08_06790"
     CDS             1331..3919
                     /locus_tag="PLK08_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPS53241.1"
                     /translation="MSIFRKTTKQRREVRKGKSSQPAWYVRYYRRPGSVVSTAILILF
                     FIVLAALDSWPENELAEYHVGQYMPSDITARQSFRVLLPAKQEELRRMAENDEPATFR
                     LNMTVLDKIVAEVEKIPVKLSTAKSIADLTPELRDELGLELPVEKRSETDEVDNAPHA
                     DIAVVASAAMPAAASQPVVVVPFSAAPTTAIAAASQPAASASQPAVVATAVKITASQK
                     LFDAWCGLTLTPEKKASFEKAVNFMRSVLMQTYIVDTPQAQYQQSRGAIYVKMTEDEG
                     KTTVTQKVVDLIGLDEKDKINQRIKLAVRQFPVSVRSSAESFLRSRLDTPLFDYDAAA
                     SESAIVAAGQAVMQNPPDECYRPYARGEIVVPRTTDDSETSKGIQPHQLACLEAENRI
                     YVSHHEAGMVFNLISRMVLFGLLVALVGWYIQNYRDELLIRHGRSLAIAVILLMMIFV
                     AKLQIYAGLPTSSLLLPIVIVTLILTIAFDQRFAMTLGSIVVAFVVILAHVGMGFFSI
                     FIAVMLVICLQIEQIHHVRTRSRIPVISVIAAIVAMVMILALCVWNNVPMRFVLWYVL
                     WGVGGIIVGGLIVQGLLPLIERIFGVATGSTLLEWCDASKPLLKRLAVEATGTYNHSL
                     QVGAMCEAAAEAIGAQGLLARVGAYYHDIGKINKPQYFSENQASGENKHEKLQPAMSQ
                     LIIQGHVKDGLELAREYGLPAALREFIASHHGTTLVQYFYQAAARRSEEGNVPKPEEH
                     QFRYPGPKPQSCEVAILMLADAAESSVRALTEPTPTRIAQQVHKIVASRLEDGQLDEC
                     DITLKEVHKIEESLTRSLCGMYHSRIPYPEDDRKKPDQPAAKSEHKPNSHQHDGEKQN
                     GSMPPQ"
     gene            3953..4303
                     /locus_tag="PLK08_06795"
     CDS             3953..4303
                     /locus_tag="PLK08_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS53242.1"
                     /translation="MEFYYHEMENDVLIISADGGLNKQTASKFVDDVGKLVDGGIKKI
                     IIDCSKLNFITSYGIGILLRIHKKLRAAGGDVKIAAVPSRIIEVLNIMKLTRIFSIYP
                     DVNRARLEFRPKDA"
BASE COUNT         1086 a         1094 c         1250 g         1042 t
ORIGIN      
        1 gtttcagagt gcggttttta acgacattct ggcgatgcgg atagacggca tagatacgct
       61 tctcgccggc gatatggcct gcaagtctga taccggcgga attttttacg tcgaaaatcc
      121 tgccgacgaa cagcctcgcg ccgaccgcta tgaaatatcg cctacaggca tggtgccggg
      181 ttatcgcagc aatttcgccg tgggccggca gggtgagatt gaacatgaag ttctgtcgcg
      241 gcgcggcgtt gagttggaca tgttcaaaaa tctcggcccg ctgaaagcta aaggtacgcg
      301 gcggatattg cggtattgtc tgcacgaacc tgatatcgac gccggcagtg acggcaacgg
      361 gacgtttata gaagtgcggt tctgtgcgcc ggctggctgt tatgcgacgt cgttgctgcg
      421 tgagatgatg aagactgaaa ttgaaggaga atgaaatgta tatcaaagga atcgacatac
      481 attcgcatgc ctttccggac gccatagctc acagggcgat ggctgccatt cagagcagat
      541 gtccggattg gccggctcgc ggcgacggca ctattgccgg ccttgccgag tccatgcgaa
      601 aaaacaatat agaaaaatcg gctgtttgca gcattgcaac caaacccggc caggctgagg
      661 gcatttgtcg atggctggct gaggtcgtgg ccgctcacga ctgctttata ccggttgcgt
      721 cggtcaatcc tgacgactct aatccgcggg attggatgaa aatgttccgt cgcgacggca
      781 tagggatagt caagctgcat ccgatgtatc agaatttcgt ggtcgacgac gaaaaaatgt
      841 atccgatata cgacgcagtc gccgaatacg atatgttgct ggaatttcac agcggcatgg
      901 acataggatt tcctgacgac gccgtacccg acagagccgc gccgcaccgt ttttcgaaaa
      961 tcgcccgaaa atttccgcaa cttaaaatgc tgctggcgca tatgggcggc ttcctgatat
     1021 gggatgacgt tgaaaaggag ctcgtcggct gtaataatat atggttcgag acctcattcg
     1081 ggctgacgca tatgtcgcgg gaacaattcg tacgcatggt tcgtaatcac ggcgaagata
     1141 aaatctgctt cggctcggat tggccgtgga ttgaccaggg cgaattgtgg gaaaaagtgc
     1201 tcgaatgcga cctcggcagc gaggccaacg cgaagatatg gcggcataat gccgcgaaac
     1261 ttctgggcgt ggggtaaaaa ggcaaaaaaa cttggacttt tccgctctcc ggagtgtaaa
     1321 atctttattt atgagcattt ttagaaagac gacgaaacag cggcgagaag ttcgcaaggg
     1381 aaaaagttcc cagccggcgt ggtatgtacg ttactatcgt cgtccaggct cagtggtatc
     1441 tacggcgata cttatcctgt ttttcattgt tctggcggct ttggacagtt ggccggaaaa
     1501 tgagctcgcg gagtatcacg tcggccaata tatgcccagc gacataacgg ccagacagag
     1561 tttcagagtg cttctgccgg ctaagcagga agaactacgc aggatggctg aaaacgatga
     1621 gccggcgacg tttcgtctga atatgacggt attggacaaa atagtcgccg aagtggaaaa
     1681 aattcctgta aaactttcca cggcgaagag tattgccgat ttgactcctg aacttcgtga
     1741 cgagcttggt ttggaactgc ccgtcgagaa gcgctcggaa accgatgaag tcgataatgc
     1801 tcctcatgcc gatattgccg tcgttgcttc tgccgccatg cctgcggccg cgagtcagcc
     1861 cgttgtcgtc gttccttttt ccgctgctcc gacgactgct attgcggcgg cgtcccagcc
     1921 tgccgcgtcg gcatctcagc cggctgttgt cgcaactgct gttaaaatca cagccagcca
     1981 gaaactgttc gatgcctggt gcggcctgac gcttacgcct gaaaagaagg cttcctttga
     2041 aaaggcggtc aatttcatgc gcagcgttct tatgcagact tacattgtag atacgccgca
     2101 ggctcaatat cagcagtccc gcggcgcgat ttatgtcaaa atgaccgaag acgagggcaa
     2161 aaccaccgtt actcagaaag tggtcgatct tatcggcctt gacgaaaagg acaagataaa
     2221 tcagcgcata aaacttgccg ttcgacagtt tccagtgtct gttcggagca gcgcggagtc
     2281 ttttttgcgg tcgcgtctgg atacgccgct gtttgactac gacgcagccg ccagcgaaag
     2341 cgccatcgtc gcagccggcc aggctgtcat gcagaatcct cctgacgaat gctaccggcc
     2401 atatgcacgc ggcgaaatag ttgttcctcg aaccaccgac gacagcgaaa cgtcaaaagg
     2461 aattcagccg catcaactgg cctgccttga agccgaaaat cgcatatatg tcagccacca
     2521 tgaagccgga atggttttta accttatttc tcgcatggtg ctctttggcc ttctggtggc
     2581 actggtggga tggtacatac agaactatcg tgacgaattg cttataaggc atggccgcag
     2641 tcttgcgata gccgttatcc tactgatgat gattttcgtg gctaaattgc agatttacgc
     2701 cggcttgccg acgtccagcc tgcttttgcc gatagttatt gtcacactga tacttacaat
     2761 cgctttcgac cagcggtttg ccatgacgct cggttcgata gttgtggcgt ttgtggtgat
     2821 tcttgcgcat gtgggcatgg gatttttcag catattcatc gcggtcatgc tggtgatatg
     2881 cctccagatt gagcagatac accatgttcg tacgcgcagc cggatacctg tcatatcggt
     2941 tatcgcagct atagttgcga tggtgatgat tttggcgttg tgcgtgtgga acaacgttcc
     3001 catgcggttt gtcctgtggt acgttttgtg gggagtaggc ggcataatag taggcggcct
     3061 gatagtccag ggcttgctgc cgttaatcga acggatattc ggcgttgcga cgggcagtac
     3121 gctgctggaa tggtgcgacg cttcaaagcc actgctcaag cggctggctg ttgaagctac
     3181 gggtacgtat aaccattcgt tgcaggtggg ggctatgtgc gaagcggcag ctgaggctat
     3241 cggcgcccaa ggactgctgg cccgcgtggg agcgtattac cacgacatcg gcaagataaa
     3301 caagccgcag tatttttccg aaaatcaggc atcgggcgaa aacaagcatg aaaaacttca
     3361 gccggctatg agtcagctga ttatccaggg ccacgtgaaa gacggccttg aacttgcccg
     3421 cgagtatggc ctgccggctg ccctgcgtga gttcatcgca tcacaccacg gcacgacact
     3481 ggtgcagtat ttttatcagg ctgccgcacg caggagcgag gaaggcaatg ttcccaagcc
     3541 ggaagagcat caattccgtt accctggccc aaaaccgcag tcgtgcgaag tggcgatact
     3601 catgcttgcc gatgcggcgg agtcgtcggt acgggcgttg accgagccga cgccgacgag
     3661 gattgcccag caggtgcaca agatagtcgc gtcaagactg gaagacggcc aactcgatga
     3721 gtgcgacatt acgctcaagg aagttcacaa gatagaggag tcgcttacgc gcagcctctg
     3781 cggcatgtat cattcgcgga ttccctatcc ggaggacgac aggaaaaagc ccgaccagcc
     3841 ggctgcgaaa tctgaacaca aaccgaattc gcatcagcat gacggcgaaa aacagaatgg
     3901 cagtatgccg ccgcaataaa aagaataatc ccttaaaagc gaggattttt taatggaatt
     3961 ctattatcac gagatggaaa acgacgtgtt gataatttct gccgacggcg gtttgaacaa
     4021 acagacagcc agcaaatttg ttgacgatgt agggaagctg gtggacggcg gaataaaaaa
     4081 aataatcatc gactgttcga agctgaattt cataaccagt tacggcatcg gcattttgtt
     4141 gcggatacac aaaaaattgc gcgccgccgg cggagacgtc aaaatcgccg ctgtgcccag
     4201 ccgtataatc gaagttctca acatcatgaa gctgaccagg attttttcca tttatcccga
     4261 cgttaaccgc gcacgcctgg aatttcggcc taaagacgcc tgaacgcaca ataaaatgtc
     4321 attcggaacg ggcataaaaa aacgacggtg aagagttttg gctcatcacc gtcgcagcag
     4381 cgtccgaggg ctgtgtttga ggttttttac tatgactcta gagcatatta aacttgactt
     4441 tgcgcttgtt gctgtttgcg gcgtcggctg gc
//
LOCUS       DAONFI010000189         4470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_23244_length_4470_cov_4.514836, whole genome shotgun sequence.
ACCESSION   DAONFI010000189 DAONFI010000000
VERSION     DAONFI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23244_length_4470_cov_4.514836"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..2005)
                     /gene="aspS"
                     /locus_tag="PLK08_06800"
     CDS             complement(161..2005)
                     /gene="aspS"
                     /locus_tag="PLK08_06800"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPS53243.1"
                     /translation="MSKDACLKRTHTCGQLRLEDNGRAVRLCGWVRSYRDHGGVVFID
                     LRDRKGITQVVFDSESVGSEMYNLAGTLRDEWVISVAGKVRPRGEGKENPKLATGQIE
                     VDCNDLVVLNRSDAVPFSPDEYHATSEENRLKYRYIDLRRQEMSGALMLRHKIVQAMR
                     GVLDGDEFIEVETPFLCKSTPEGARDFLVPSRMHRGTFYALPQSPQLYKQILMVGGLE
                     KYYQVARCFRDEDLRADRQPEFTQLDLELSFCDEQDVMNVTNRVLRAVCKVAGKYFPD
                     EVPVMTYQEAVARFGSDKPDLRFGMEIKDISDIAAKTDFRVFSSAVENGGAVQCIVVP
                     GGARMTRKETDALADWAKTFGAGGMPITKVAGGKLETGVAKFLEKIATQLIERLDAKD
                     GDLICFAADRLKVVQRVLGELRCKLARDLGMIDPNDFKWLWVVEFPLFEYDEDAGRYV
                     AMHHPFTCPNIDDWEKYRDSDPLKIRSRAYDIVCNGMEMGGGSIRIHRTDLQKDVFTF
                     MGIKDEEASLKFGFLLDALRFGAPPHGGLALGLDRVVMTMIGAPSIRDVIAFPKTQKG
                     TCPMTEAPSFVDEKQLEELFISVVEEDGDVQKSSVSDLDLPPDLDDIM"
     gene            complement(2048..>4470)
                     /locus_tag="PLK08_06805"
     CDS             complement(2048..>4470)
                     /locus_tag="PLK08_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPS53244.1"
                     /translation="KQLKPVHWSIDNRTALADAELEYYDREDASIFVAFRLVDVKATL
                     AGVAPSTPMYVVIWTTTPWTLVANRAVAVHPDFEYAAVQVDSPLGRVVVFVAAERLEA
                     LKAAGEKWMTGAEVLATCKGSDLVKAQLRYAHPLSAGVTCPVVAANYVTLEDGTGLVH
                     TAPGHGTDDYYTGLNNNLEIYCPVQADGTYDMTAPAWLQGKSVWDGNGLVVEYLEKQK
                     TLVAIHMMRHSYPHDWRSKTPTIFRATEQWFIGVDKPLKNGGHTLRQMALDVCGKALA
                     QGGVDFIPSWGRNRILGMLESRPDWCLSRQRSWGLPITAFYNPQGRVLCTPFSVREVA
                     KTIRALGSDAWFNETPKTLLGDYRPQDDTDLVNADEFDVETLEKGYDIFDVWFESGSS
                     WYAVAVARGVVEEPPVDLYLEGSDQHRGWFQLSLLPCLGVAGTAPFKKVLTHGFVVTD
                     SGHKMSKSLGNTIEPQEQLTRRGADILRLWVASQNYQDDIRCSEKLIEQAEDAYRKIR
                     NTIRFCMGSIGDYDPAKDYVAPMEHSVGRWMQIELQLLIKNVRDAYDKFEFHRATRLL
                     YEFCTIQASSVYLTAVKDRLYCDLPDSPRRRACQGIIRSVLIALVKLAAPILPHTCEE
                     AWRQIPHRDAGEPDSVHLSFLPKADEKFIRLLEDVTPIVDDDLIVQGENLQIGPAWIW
                     ERLMDIRGEALVMLEDLRNRGVKNQLDAEVIFTVSPQRPGVKKFMQLYVGELEDMLGV
                     GYACVVDGSAPEGRLVGIQVLDAREKYNRCERSWKRRPDVGSDSEYPTLSARDAAVMR
                     QMKNRKKS"
BASE COUNT          988 a         1314 c         1121 g         1047 t
ORIGIN      
        1 gcccgcaaaa ttgctcgatt tcggcgacgc ccatgttttt gggataggtt ctataataaa
       61 aaagctggct acgtcatccg taaccagctt ttttattatt tttttacatt ttatatcaga
      121 gcagttttca aaatgaaatt tcttttgaga accatatact ctacattatg tcatccaggt
      181 caggcggcaa atccaggtct gaaactgaac ttttttggac atcgccatcc tcttccacga
      241 ccgatatgaa aagttcttca agctgctttt catccacaaa tgaaggggcc tccgtcatgg
      301 ggcatgtacc cttctgcgtc ttgggaaacg ctataacgtc acgtatgctc ggtgcgccta
      361 tcatggtcat tacgacgcgg tcaaggccga gagccaatcc gccgtgcggc ggcgcgccga
      421 atcgcaacgc atccagcaaa aagccgaatt tcaggctggc ttcctcatct ttaatgccca
      481 tgaacgtaaa cacgtctttc tgcaaatcgg tccgatgtat acgaatactg ccgccgccca
      541 tttccatgcc gttgcaaacg atatcatatg cgcgggaacg gattttcaac gggtcgctgt
      601 cgcgatattt ttcccagtca tcgatattcg ggcaggtaaa agggtggtgc atggccacgt
      661 aacggccggc atcctcgtcg tattcaaaca gcggaaactc gaccacccac agccatttga
      721 aatcgttggg gtctatcatg ccaagatccc tggctaactt gcaacgaagc tcaccaagaa
      781 cgcgttgaac gactttcagc ctgtcggctg cgaagcagat gaggtcgccg tcttttgcgt
      841 cgagacgctc aataagttgc gtcgcgattt tttccagaaa tttcgcaact ccagtttcca
      901 gcttgccgcc ggcaactttg gtaatcggca tgccgccagc gccgaacgtc ttggcccagt
      961 cagccagtgc gtcggtctcc ttgcgggtca tcctggcgcc gccgggaacg actatacact
     1021 gcacagcgcc gccgttttcg acggctgaac tgaagacgcg gaaatcggtt ttcgcggcga
     1081 tatccgaaat gtcctttatt tccatgccga aacgcaaatc gggcttgtcc gaaccgaagc
     1141 gggccacagc ctcctgatat gtcatgaccg gtacttcatc cggaaaatat ttgcctgcga
     1201 ctttgcagac cgcacgaagc acgcgattcg taacattcat cacgtcctgt tcatcgcaga
     1261 aactgagttc caggtcaagc tgcgtaaatt ccggttgacg gtctgcgcga aggtcttcgt
     1321 cacggaagca gcgggcgacc tggtaatatt tttcaaggcc gccaaccatc agaatctgct
     1381 tataaagctg cgggctctgc ggcaatgcat aaaaagtgcc gcgatgcatg cgcgacggca
     1441 caagaaaatc gcgcgcacct tcaggcgtgc tcttgcagag gaacggcgtt tcaacttcga
     1501 tgaactcgtc gccatcaagc acgccccgca tcgcctgcac tatcttatga cgcaacatca
     1561 gcgcgccgga catttcctgg cgtcgcaaat cgatataacg atattttaag cgattttctt
     1621 cgctggtggc gtgatattca tccggcgaaa acggcacggc gtcggaacgg ttcagtacga
     1681 ccaggtcatt gcagtcgact tctatctggc cggtggcgag cttcggattt tccttgccct
     1741 cgccgcgagg ccgaactttg cccgctacgc ttatgaccca ttcgtcgcga agcgtgccgg
     1801 ccagattgta catctcgcta ccgacgctct cgctgtcaaa caccacctgc gtaatgccct
     1861 tgcggtcgcg caggtcgata aaaacaacgc cgccgtggtc acgataactc ctgacccacc
     1921 cgcagaggcg aaccgccctg ccgttatctt caagccgtaa ctggccacaa gtgtgggttc
     1981 tttttaaaca tgcatcttta ctcatctgaa tattttcctg atatttttat cacaatagaa
     2041 gggcttttca gcttttcttc cggtttttca tctgtcgcat aactgccgca tcgcgtgctg
     2101 aaagcgtcgg atattcactg tcgctgccga cgtccggccg acgtttccag cttctttcgc
     2161 aacgattata tttctcgcgg gcatccagta cctgtatgcc tacgagtctg ccctccggcg
     2221 ccgagccgtc caccacgcag gcgtatccaa cgccgagcat gtcttccagc tcgccgacgt
     2281 ataattgcat gaattttttc acgcccggcc gctgcggcga aacggtaaag ataacttccg
     2341 cgtcgagctg attcttcacg cccctgtttc gcaggtcttc aagcatgaca agcgcctcgc
     2401 cgcgaatatc catcaatcgt tcccaaatcc acgccgggcc tatctggaga ttttcgccct
     2461 gaacaatcag gtcgtcatcg actatcggcg tcacatcctc cagcagtcgg atgaattttt
     2521 catcggcttt cggcagaaac gacaggtgta ccgaatccgg ctcgccggcg tcacggtgcg
     2581 gaatctgtcg ccacgcctct tcacaggtat gcggcagaat cggagccgcc agtttgacaa
     2641 gcgctataag tacgctacgg ataataccct ggcaggcacg gcggcgaggc gaatcgggca
     2701 ggtcgcagta cagcctgtcc ttcaccgcag tcaggtacac gctcgacgcc tgtatcgtgc
     2761 agaattcata caacagccgc gtcgcgcggt gaaattcaaa tttgtcataa gcgtcacgca
     2821 catttttgat gagcagttgc agttctatct gcatccagcg gccgacggaa tgctccatag
     2881 gagccacgta atctttcgcc gggtcgtaat cgccgattga ccccatgcag aaccggatgg
     2941 tgttgcgtat tttacgatag gcatcttccg cctgttcgat gagtttttcg ctgcaacgga
     3001 tatcgtcctg ataattctgg ctggccaccc acagacgcag aatatcggcg ccgcgtctgg
     3061 tcagctgctc ctgcggttcg attgtattgc cgagcgattt gctcatcttg tggccgctgt
     3121 cggtcacgac gaatccgtgc gtcaggactt ttttaaacgg cgcagtaccc gccacgccga
     3181 ggcacggcag aagtgacagc tgaaaccagc cgcgatgctg gtccgaacct tccaggtaga
     3241 ggtctaccgg cggttcttcc acaacgcctc gcgcgaccgc aacggcatac cagcttgagc
     3301 cgctctcaaa ccacacgtcg aaaatgtcgt aacccttttc gagcgtctcg acgtcaaatt
     3361 catcggcatt tacgaggtcg gtatcatcct gcggcctgta atcaccaaga agcgttttgg
     3421 gcgtttcatt gaaccacgca tccgaaccca gcgcacggat tgttttggcc acttcacgaa
     3481 cgctgaacgg agtgcacagg acacgtccct gcgggttgta gaaagcggtt atcggcagcc
     3541 cccacgagcg ttgtcgggag agacaccagt cgggccggct ttcgagcatg cccagtatac
     3601 gattgcggcc ccacgaagga atgaaatcca ctccgccctg ggccagtgct ttgccgcaga
     3661 cgtcgagagc catttgccta agcgtatggc cgccgttttt aagcggcttg tcaacgccga
     3721 taaaccactg ctcggtcgca cggaaaatcg tcggcgtctt gctgcgccag tcgtgcggat
     3781 aagagtgacg catcatatga atcgcaacaa gcgttttctg cttttcgagg tattcgacca
     3841 ctagcccgtt gccgtcccag acgctcttgc cctgcagcca ggccggcgcc gtcatatcat
     3901 acgttccgtc agcctgcacg gggcagtaaa tttccagatt attgttcaag ccggtgtaat
     3961 aatcgtccgt accgtgtcct ggtgcggtgt gcacaaggcc agtgccgtct tcaagcgtca
     4021 cgtaattagc agccactaca ggacatgtta cgccagccga caacggatga gcatatcgca
     4081 actgagcttt cacaaggtcg ctgcccttgc aggtagccag aacttctgca ccggtcatcc
     4141 atttttcgcc ggcggctttc aacgcctcaa gccgttcggc tgcgacaaaa accaccacac
     4201 ggccaagcgg cgaatcaacc tgaacggcgg catattcaaa atcgggatga acggcgacgg
     4261 cacggttggc gacgagcgtc cagggcgtag tcgtccatat caccacatac atcggcgtcg
     4321 acggagcaac gccggctaac gtagccttca catccacaag gcgaaacgcc acgaatatgc
     4381 ttgcgtcttc acggtcataa tattccagtt ccgcatcagc cagcgcggta cggttatcta
     4441 tcgaccagtg caccggcttg agctgcttgt
//
LOCUS       DAONFI010000190         4457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_23341_length_4457_cov_4.283735, whole genome shotgun sequence.
ACCESSION   DAONFI010000190 DAONFI010000000
VERSION     DAONFI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23341_length_4457_cov_4.283735"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLK08_06810"
     CDS             <1..1181
                     /locus_tag="PLK08_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPS53245.1"
                     /translation="GFYIMRTGWKPQDKYLFFDCVPSGDFWSHWHNSAFNVDIYAYGR
                     PLIVDSGMQSYDEPSYEDYCHRSIAHNIIEIANVEVLPVPELKYWLRSPEFCVIDAQA
                     NITIHYGHRILITRRVLFVTGDYWIVDDIVEHGPMSQRSNLPVRAYWHLNSRSVVADG
                     KQIALAVTDKDDGSGRHYINSGKDKNLSFYTNDKNIGNLLFIPDKQEDYKSLAIMSDT
                     PCGGYVACYRQDLIPSKNPTRYFTSLLYPFEGDKRPDVSFKDGVVKIGKNREGVYMRK
                     GEKTDGDCAFVGKEDGAINRILLVAGSRISDAVQLDVSADFMIVTRQGESLDIQLMES
                     PSKKVNRIELAGLQGVSKVKVNGKSCKVSETDGKLVIVGPFEKIVPNPDNPDLFWIVK
                     "
     gene            complement(1518..2891)
                     /locus_tag="PLK08_06815"
     CDS             complement(1518..2891)
                     /locus_tag="PLK08_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HPS53246.1"
                     /translation="MLRTEEILSTIRMLHSEHLDVRTVTLAINVDDCSATSCDTLCRK
                     VYDKVMSLAGNLVETCDNIGRKYGIPVTNKRIAISPASRLLAGHGRATALELAVTLDR
                     AAERCGVDLVGGFSAHVQKGITNSSDLLMESLPEVLSHTKRVCSAINVASRKAGINMD
                     AVAKMGRLIPQIAQATADSRGFGAAKLVVFANVPEDNPFMAGASMGTGEPEATINIGV
                     SGPGVICSALKRRIESGEKLTLGDLAEEIKITSFRVTRSGELIGREVADELGLPFGIV
                     DLSLAPTPTIGDSVGEIIKALGIANVGAPGSTAAVAMLNDAVKKGGAFASSSVGGMSG
                     AFIPVSEDSTLAQAVADGQLVLEKLEAMTSVCSVGLDMIAIPGDTSPQTIAAIIADEM
                     AIGVINNKTTAVRVIPVPGAKAGDFVEFGGLFGSATVIEIRNMGASDAFVNFGGRIPA
                     PIQSLGN"
     gene            complement(3041..3607)
                     /locus_tag="PLK08_06820"
     CDS             complement(3041..3607)
                     /locus_tag="PLK08_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPS53247.1"
                     /translation="MKTGYKYAYVLDVMSNDHPGIVAAVSDEISSRNGNIDAVSQTVL
                     QGYFSLIIIVSFPAAISAEELSAAIEAHNESFQVMARPYQAEKSVTEAADTFVITAFG
                     EDCEGIVHKFSHYLAGKDVNIIDLYGDHNGSEFVLIGQVQVPRRWDINHLQADLEEMG
                     RESGYTVKLQHENIFVATNQLRFKSQKS"
     gene            3783..4355
                     /locus_tag="PLK08_06825"
     CDS             3783..4355
                     /locus_tag="PLK08_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS53248.1"
                     /translation="MGEEKNFQLGVKVRRLRLAGHLTLDSLSRLAGVSKAMLSQIEQD
                     KVNPTVAVIMKIAAALQVNISDLLDQPAKKGILRIIRHDDPAYTYRSDQCCNIRTLSP
                     LSLEKSIEFYSVSLEPDGVIKSEPHFPGTEEILHVTRGKVTVTSGQETIELVHGDSAH
                     YRADVEHCITNNSRSRSEFFLVVKYHEIGA"
BASE COUNT         1138 a         1110 c         1058 g         1151 t
ORIGIN      
        1 ccggttttta tataatgcgt accggctgga agccgcagga taagtatctt ttctttgatt
       61 gcgttccaag tggcgatttc tggagtcatt ggcataatag tgcttttaac gtcgacattt
      121 acgcatatgg ccgtccgctt atcgtggatt ccggcatgca atcatatgat gaacctagct
      181 atgaagatta ttgtcataga agcatagccc acaacatcat cgaaattgct aatgttgaag
      241 ttttgcctgt tccggaattg aaatattggc tgcgttcgcc ggaattttgt gtcatagatg
      301 cacaagctaa tataactatc cattatggtc atagaatact aataactcgt cgtgtacttt
      361 ttgtgacagg tgattattgg atagtggacg atatagtaga acatggcccg atgtcacaac
      421 ggtccaatct tcctgtaaga gcgtattggc acttgaattc aaggtctgtt gttgcagatg
      481 gcaagcagat tgccctggcg gttacggata aggatgatgg gagtggtcga cactatataa
      541 attcgggcaa agataagaat ctttcttttt atactaacga taagaatata ggaaaccttt
      601 tgtttatacc cgataagcag gaagattata agtcgcttgc gataatgtcg gatacgccct
      661 gcggcggata tgttgcctgt tatcggcagg acctgattcc gagcaaaaat ccgacgagat
      721 attttacgtc attgttatat ccgtttgaag gcgataaacg tccggacgta tccttcaagg
      781 acggcgtggt caaaatcggc aaaaatcgcg aaggcgtcta tatgcgtaag ggcgagaaaa
      841 ccgacggcga ttgtgcgttc gtcggcaaag aagatggagc gattaatcgc attcttcttg
      901 tcgccggcag ccgcatatct gacgcggttc agcttgacgt ttctgccgat ttcatgattg
      961 taacgcgaca gggcgagtcg ctggacatac aattaatgga aagtccgtcc aaaaaagtta
     1021 atcgcataga gttggctggc ttgcaaggcg tcagcaaagt aaaggtgaac ggcaaatcat
     1081 gcaaggtatc cgaaactgac ggcaaattgg taattgtcgg gccgttcgag aaaatcgttc
     1141 ccaaccccga taatcctgat ttattctgga ttgtaaaata aaagctctgg tcatcgccaa
     1201 cggcttaatt ttactattcc gcatgaatta atgttgaaat cagctgcgga tgaacgataa
     1261 gaaaatggat atatgcacgt caggctttta tagcaatttt ggagtggttc tcaaaatgaa
     1321 attttttttg agaaccactc ttagagcatg ttaaacttga gatacgacac tttatgcgtt
     1381 ggcgaaacga gctatgcaag gaaggcgaag caggaatact gcccgtattc cgatgcagcc
     1441 tgacgtagca gagcgatgtt gcagccaaca aaaaggacgc atatcaagtt taaaatgctc
     1501 tagcccaagc cgcaatatca atttcccaat gattgaattg gtgcgggtat tcgtccgccg
     1561 aaattcacga acgcatcgct tgcgcccatg ttacgtattt ctataacggt ggcagaaccg
     1621 aacaggccgc cgaattccac gaaatcgccg gctttcgcgc ctggtacggg tataacgcgg
     1681 acggcggttg ttttgttgtt gattacgccg atagccatct cgtcggcaat tattgcggcg
     1741 atggtctgcg gcgaagtatc gcctggtatg gcaatcatat ccaggccgac ggaacagacg
     1801 ctcgtcatgg cttccagttt ttcgagcaca agttggccgt cggcgaccgc ctgtgccagc
     1861 gtggaatctt cactgaccgg tatgaacgcg ccgctcatgc cgccgacgct gcttgacgcg
     1921 aacgcaccgc cctttttcac cgcgtcattg agcatcgcaa cagctgccgt cgaaccaggc
     1981 gcgccgacat tcgcaattcc cagcgccttg attatctcgc caaccgagtc gcctatcgtc
     2041 ggcgtcggcg ccaacgacag gtcgactatt ccgaacggca ggccgagttc gtcggctact
     2101 tctctgccga tgagttctcc gctgcgggtc acgcgaaaac tcgtaatctt gatttcttcg
     2161 gcaaggtcac cgagcgtgag tttttcgccg ctttctattc tacgcttgag tgcggagcat
     2221 atcacgccag ggccgcttac gccgatattg attgtcgctt ccggttcgcc cgtccccatc
     2281 gaagcgccag ccatgaatgg gttgtcttcc ggcacatttg caaagactac cagtttagct
     2341 gcaccgaatc cacggctatc cgcggtggct tgggcgattt gcggtatcag tcggcccatt
     2401 ttagccacgg catccatgtt gatgccggct ttccggctgg cgacgttgat agccgaacac
     2461 acgcgtttcg tatggctgag cacttccggc agcgattcca tcagcaggtc gctcgaattg
     2521 gttattccct tctgtacgtg cgcggagaaa ccgccaacca gatccacgcc gcagcgttcc
     2581 gcagccctgt cgagcgtgac ggctaattcc agcgccgtcg cccggccatg gccggccaat
     2641 agccgcgacg ccggcgaaat ggcgattcgc ttgttcgtca caggtatgcc gtacttcctg
     2701 ccgatattat cgcaagtttc caccagattg ccggccaacg acatcacctt gtcatagact
     2761 ttgcggcaga gcgtgtcgca actggtggca gaacagtcgt ccacgttgat tgccagcgtc
     2821 accgttcgaa cgtccagatg ctcgctgtga agcatccgga tggtacttaa aatttcttca
     2881 gtcctaagca ttacaataaa atccttaaaa ttgaatttct atccgttagt gagattttaa
     2941 caatcaattt atttcccaaa ccattctaaa atccattaga gtcttttcac atggcaaacc
     3001 agtggaattc tctaaggaag cattaaaaaa tggttcactt ttaacttttc tggcttttga
     3061 atctcagctg attcgtcgcc acgaaaatat tttcatgttg gagctttacc gtatagcccg
     3121 attcgcggcc catctcctcc aggtcagcct gcaagtggtt tatatcccac ctgcgcggca
     3181 cctgtacctg gccgataagt acgaattcac tgccgttgtg atcgccgtaa agatctatta
     3241 tgttcacatc tttgccggcg agataatgac tgaatttatg cactatgcct tcgcaatctt
     3301 caccgaacgc agttatcacg aaagtatccg ccgcttcagt gacagatttt tccgcctggt
     3361 aaggccgcgc cataacctgg aacgactcgt tgtgagcttc tattgccgcc gacaactcct
     3421 ctgccgaaat cgccgccgga aaactcacta taattatcag cgaaaaatat ccctgcaaaa
     3481 cagtctggct gacggcgtct atgttcccgt tgcgagagga tatctcgtcg ctgaccgcgg
     3541 cgacaatgcc gggatggtcg ttgctcatca cgtcaagcac gtatgcgtat ttgtaacccg
     3601 ttttcatttt cacctcgtaa aatccattta tgtatctgca acaagtctat tgcgccgcaa
     3661 acgcaatgtc aaagccgcaa tattttcttg attgtcatcc tgcttgagta taatatataa
     3721 atctgaaatt taatatagtg aaattcagta tatcaaactt tttgttcgag tcaataaaca
     3781 ttatgggtga agaaaaaaat tttcagctcg gtgtaaaagt tcgtcggtta aggcttgccg
     3841 gccacttaac gctcgattcg ctttcgcggt tagccggcgt gtcgaaagcc atgctcagcc
     3901 agattgaaca ggacaaggtc aaccccacag tagccgtcat catgaaaatc gcagccgccc
     3961 tccaggtaaa catatccgac ctgcttgatc agccggcaaa aaagggtatt ttgcgaataa
     4021 tccgtcacga cgaccctgca tatacatatc gcagcgacca atgctgcaat atccgcacgc
     4081 taagcccgct gagccttgaa aagtccatcg aattttacag cgtctcactt gagccggacg
     4141 gagtcataaa atccgagccg catttccccg gcacggaaga aatattgcac gtcacgcgcg
     4201 gcaaggtcac agtcaccagc ggccaggaga ccatcgagct tgttcacggc gactcggcac
     4261 attaccgtgc cgacgtggaa cactgcatca caaacaattc ccgcagccgc agcgaattct
     4321 tcctggtcgt gaaatatcac gaaatcgggg cataaaataa taaaaaattt tcaaataaca
     4381 agaaacacaa gctacaagta aattctagag catgttaaat ttgaaattgc gccttgttgc
     4441 gtcgttgagg acgaggc
//
LOCUS       DAONFI010000191         4455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_23358_length_4455_cov_4.197045, whole genome shotgun sequence.
ACCESSION   DAONFI010000191 DAONFI010000000
VERSION     DAONFI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23358_length_4455_cov_4.197045"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLK08_06830"
     CDS             <1..340
                     /locus_tag="PLK08_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS53249.1"
                     /translation="DKCHVITCREKSFTIRNAPRIFNIIYSKLIPNIDIVLVLTSVEI
                     VDSSACGCILHLHRLLKAGGCRLAVVCKGNVRNTLMRLHFQRLMHLFPQTSDAVKAIR
                     KSRAAASEIK"
     gene            722..2140
                     /locus_tag="PLK08_06835"
     CDS             722..2140
                     /locus_tag="PLK08_06835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS53250.1"
                     /translation="MFQNMEKLRNSDLTDMIAKTITTTAVICFLFVALFTLEDLTNFV
                     QVLKAQRWSYMMLLLGQALLTINIGALIWRIILVWSYKPARECTDDELPMCTVIVPAY
                     NEGHQVYDTIVSLVKSDYPHDKLEIIAVNDGSKDDSWYWMHKAAQEFPDIVDPINCVM
                     NRGKRQALNEGFLKARGSVIVTVDSDSEVEVQTLRRMVSPMVLDRKVGGVAGNVRVLN
                     RRDGILPRMLDVSFTYSFDFIRASQSYVNSVLCTPGALSAYRADIVNSVREEWLKQTF
                     CGRPANIGEDRAMTNLILRNGYHVHFQQDAIVYTKVPTDYKTLCKMFLRWARSNVRET
                     LVISGFAFKKFRPTPATGTRVNLLLQIIQMTLPILLQIQTVFCLIAAPFIFGTHLIFG
                     AMIASLIPIAVYTWRHRNGNGLWAIPYSVFWVFGLSWIAPYALFTAHKGGWLTRELPA
                     NAEQKQPAIINAIHPSLTEVNL"
     gene            complement(2361..3950)
                     /locus_tag="PLK08_06840"
     CDS             complement(2361..3950)
                     /locus_tag="PLK08_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53251.1"
                     /translation="MTGRILVAGVCAVFFLGISAQAATVTPTQEQKAALTKAVNDFNL
                     VNRQMRNMNVVRDGQAVLGKLDGVEKTLFDAGWQRTDAQADKVFKAVDDARGRLKAAM
                     EKHGVSSSSEPAAAAKLRYDQQRSLSDAKLNLRNVEAGLDNARQTAEALKAGKNVPQN
                     DMETATNSLTAANKSMDKVRKNIKQLPASHPDVKPVYDAAVKAEDTLKEYTDIVMGKV
                     KDVGQEMRKLVDDVADDYARLSRMAQAYREASDKKFMDYVSKYDYPKYRQLWIDAAMA
                     QGHANEMKKKYAVFADKHGGMAETGKKVLNSADDVQKYAPIFVDNITAAMAKDITNVN
                     DAMDSVKDEAKYAVDNKKPAHFAGGIPQKMNEIADHITQLEQAPVVPHKTGDLRKRYN
                     ELVADIDRQKADMEAEIIAEVKLPADEYKGSDRNEVEKMIRDEWAQKYPNEKIELIRF
                     HSPVWYVTREWRWDDGNKAWVWYDRAVLPVKVVVKNADGKTANIYMAFCNRDNVNKTM
                     NAGVETRDGYVVETILMENVK"
     gene            4252..>4455
                     /locus_tag="PLK08_06845"
     CDS             4252..>4455
                     /locus_tag="PLK08_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HPS53252.1"
                     /translation="MGYNIVVCAKQVPDTKRITGEAMKEDGTVNRAALPAIFNPEDLN
                     ALEAALAIRDEHGGTVTVITMGPP"
BASE COUNT         1064 a         1158 c         1012 g         1221 t
ORIGIN      
        1 tgataaatgt catgtgataa catgccgtga aaaatcgttc actatccgta acgctcccag
       61 aatttttaat atcatctaca gcaaactcat tccgaacatc gatatcgtat tggttttgac
      121 gtccgtggaa atagtagatt cttccgcatg cggatgcatt cttcatcttc accggctgct
      181 caaagccggc ggttgcaggc tggcagtagt atgtaagggt aatgtacgta ataccttgat
      241 gcgcctgcat tttcagcggc tcatgcacct gtttccgcaa accagcgatg ccgtcaaggc
      301 aatcaggaaa tccagagctg cggcatcaga aataaagtaa cccgaatttt ctgaatcgtc
      361 ggagcagaaa tttcccgtaa ctatctgcaa ataagtttta tttgactttt cataattagt
      421 ggtataatac tctgcttggc tggaatagta ctgccaatcc tgcaacgctg ggatttagca
      481 agggtcattg ccataaattg tttattgtaa gccctgaagt acattcgtat cttactatat
      541 ttgaaaggaa tagtagtgcc gcaccaacac tgcaacttgt aacagtgttc gcttcctccc
      601 agctggaggc tacgtgcata catgaccttt ccacaccact gccatgaagc aatcaatatt
      661 caaaaaaaac accaaggtaa atgttccttc gcgtttctcg tccaaaaaga tgaggaagtc
      721 gatgtttcag aacatggaga agctcagaaa ttccgacctg acggacatga ttgccaagac
      781 tatcaccacg acagccgtca tatgttttct tttcgtggct ctcttcacgc ttgaagacct
      841 cacaaacttc gttcaggttc tcaaggccca acggtggtcg tacatgatgc tgttactggg
      901 ccaggcgttg ctgacaatca acatcggcgc actcatatgg cgcataatcc tcgtctggtc
      961 atacaagccg gctcgtgaat gcacggacga cgaactgccc atgtgtaccg taatagtgcc
     1021 agcctacaac gaaggccacc aggtttacga taccatcgtc agcctcgtaa agagcgatta
     1081 cccccatgat aaactggaga ttatcgccgt aaacgacggt tcaaaagacg acagctggta
     1141 ctggatgcac aaggcggccc aggaattccc tgacatcgtc gacccgataa attgcgtgat
     1201 gaatcgcggc aaacgtcaag ccctcaacga aggcttccta aaagctcgcg gcagtgtcat
     1261 cgtgacagtc gacagcgaca gcgaagtgga agtccagacg ctccgtcgca tggtcagccc
     1321 aatggtactc gacagaaagg tcggaggcgt agccggcaat gttcgcgtgc tcaaccgccg
     1381 cgacggcatt ctcccaagaa tgctggacgt gagtttcacg tacagctttg atttcatccg
     1441 tgccagccag agttacgtca acagcgtgtt atgcacgcct ggcgcgcttt cagcctaccg
     1501 tgccgacata gtgaattccg ttcgcgagga atggctgaaa cagacgttct gcggccggcc
     1561 ggctaacatc ggcgaagata gggccatgac caatcttatc cttcgcaatg gctaccacgt
     1621 gcattttcag caggacgcca ttgtatatac gaaagtgccg accgattaca aaactctctg
     1681 caagatgttt ctccgttggg ctcgcagcaa tgtcagggaa acgcttgtaa taagcgggtt
     1741 cgcctttaaa aaattccggc cgacgccggc taccggcaca agagtcaacc tgttgctgca
     1801 aatcatacag atgactcttc cgattttgct gcaaatccag acggtatttt gcctgatagc
     1861 cgctccgttc atattcggaa cgcatctcat tttcggggct atgattgcat ctttaatccc
     1921 catagctgtt tacacatggc gacaccgtaa cggcaatggc ctttgggcga taccgtattc
     1981 ggtattctgg gtattcgggt tgtcgtggat agctccatat gccctgttta cagcccataa
     2041 gggcggctgg ctgaccagag aactgcctgc aaatgccgaa cagaaacagc ctgcaataat
     2101 aaacgctatt cacccgtcac ttacagaagt caacctgtaa cacttcaaaa cagtacggca
     2161 gctataaaaa attacggtac ggctttagga gttttagcgc attaaaattg attgcgattt
     2221 gttgcgccga caaaacatct ttgcacgcaa gtttcaattt tcaagtattg cttgtatccg
     2281 taaaaaaact tgaaacctgt ttctttgaat ttgcttttaa cttgtcatcc tgttttttga
     2341 aacgttttcc gttcagattc ctatttgacg ttttccatca ggatggtttc cacaacataa
     2401 ccatcgcggg tctctacgcc ggcattcatc gttttgttga cgttatcgcg attgcagaac
     2461 gccatataaa tattcgccgt tttcccgtcc gcgtttttca ccaccacttt caccggcaga
     2521 accgccctgt cgtaccagac ccacgctttg ttgccgtcgt cccatcgcca ttcccgggta
     2581 acgtaccata ccggcgaatg gaagcgtatc agttcgattt tttcattcgg atatttttgg
     2641 gcccattcgt cgcgaatcat tttttccact tcgttgcgat ctgagccttt gtattcatct
     2701 gccggcagtt tgacttcggc gattatctcc gcttccatat cggctttttg cctgtcgata
     2761 tccgcaacca gctcattata tcgtttacgc aaatcacctg ttttatgggg aacaaccggc
     2821 gcctgctcaa gctgggtgat atggtcggcg atttcgttca ttttttgcgg gatgccgccg
     2881 gcaaaatgtg ctggcttttt attatcgacg gcatatttcg cctcatcttt gacggagtcc
     2941 atcgcgtcat tcacattcgt aatatccttt gccatagccg ccgtgatgtt atccacaaat
     3001 atcggggcgt acttctgaac atcatcggca gaattgagca cttttttgcc cgtttccgcc
     3061 atgccgccat gtttgtcggc aaaaacggcg tattttttct tcatttcatt agcgtggccc
     3121 tgcgccatcg cggcgtctat ccacaactga cgatatttgg ggtaatcata tttactgacg
     3181 tagtccatga attttttatc ggacgcttcc ctgtacgcct gcgccatgcg agacagcctc
     3241 gcataatcat cagccacgtc gtcgacgagt ttccgcattt cctgcccgac atccttaacc
     3301 tttcccatca caatgtcggt atattccttc agtgtatctt cagctttgac ggcggcatcg
     3361 taaaccggct tgacatccgg gtggctggct ggaagctgtt tgatgttttt gcggacttta
     3421 tccatacttt tatttgcggc ggtgaggctg ttggtcgcag tttccatgtc attttgcggg
     3481 acgttttttc cggctttcag tgcctcggca gtctggcggg cattatccag gccggcttcg
     3541 acgtttcgca ggttgagttt tgcatcgctg aggctgcgct gctggtcata gcgaagtttg
     3601 gcggcggcag cgggttcgga cgacgacgat acgccgtgtt tttccatggc ggctttcagg
     3661 cgtccacggg catcgtcgac agccttaaaa accttgtcag cctgggcatc ggttctctgc
     3721 cagccggcat caaacagcgt tttctcaaca ccgtcaagtt ttcccaaaac agcctggccg
     3781 tccctgacga cgttcatatt ccgcatctgc ctgttcacga gattaaaatc attgacagcc
     3841 ttggtaagag cagctttctg ttcctgcgtc ggagtcaccg tcgccgcctg agcagatatt
     3901 ccgagaaaaa ataccgcaca gacgcctgca actaaaattc tgcctgtcat ttgccagtct
     3961 ccttttgatt ttttttgacc aaatgccttc gtaccgtttc ctggattata ttcaccagcc
     4021 cattacggaa caagcctcca caaaagcaat tatgacaaat taatcgtaaa tttccgtcat
     4081 tattggagta agattcgttc atctcaaacc ttttttaaag aattcgcaaa tatcttgact
     4141 aatttgcgtt gataatctaa tattttgcga ttcaagatac gaccctacgg cagtccgttg
     4201 cagaagatgc gtcatcttca gttcggtctg cgtttatgca ggagtagcac attgggttac
     4261 aacatagttg tttgtgcaaa acaggtgccg gacaccaaac gcataaccgg cgaggcgatg
     4321 aaagaagacg gcacggtcaa ccgtgcggca ttgcctgcta ttttcaatcc cgaagacctc
     4381 aacgcccttg aagcggcgtt ggcgatacgc gacgaacacg gcggtacggt aacagtcatc
     4441 accatggggc cgccg
//
LOCUS       DAONFI010000192         4441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_23471_length_4441_cov_4.046056, whole genome shotgun sequence.
ACCESSION   DAONFI010000192 DAONFI010000000
VERSION     DAONFI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23471_length_4441_cov_4.046056"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..>987)
                     /locus_tag="PLK08_06850"
     CDS             complement(38..>987)
                     /locus_tag="PLK08_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53253.1"
                     /translation="KKAGNKYVADVRIFNTLTNPQTVVKNTFSDETERWRGNIAQKII
                     QAFIGRQTEQPPQAGDEQFPSADRLTGPINANPSFELAGHGGWESPDNVASFIVERPD
                     NSGKMLLIRTDVDREQWLAYRKALSAGEASVDSPPQITSGKNDTIAGQEGVHFKSDFI
                     PADTNATYWLKASCRFIQPSNPRASATSKVFIKGFRKIPLANINGLPESTLKKLKMTP
                     ADFANLPPMKQRELIEADARRNPQNYLRECYRWHMNCEVTSAWKTFDGKFPPRDRLPD
                     NVQFLQIQIYSYWPPGEYFWDDVELYIEPIPKTSAACNR"
     assembly_gap    988..997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<998..1394)
                     /locus_tag="PLK08_06855"
     CDS             complement(<998..1394)
                     /locus_tag="PLK08_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53254.1"
                     /translation="MRKTGRKPAHQPADSRANKSMNSIKRAQSGTFRTAAVAVLLWLI
                     CFAAVRTFGETGDGDVFVAVFDFSPQSEDAAALADAVRLKLARQDGRWKVIDTQSMRE
                     LAISSPDMNSTAMSLRTLLQEQVAAHACIF"
     gene            complement(1339..3774)
                     /locus_tag="PLK08_06860"
     CDS             complement(1339..3774)
                     /locus_tag="PLK08_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53255.1"
                     /translation="MKKAAVLGMMILATSSLVFAADAGVEKTLEINSILADAQSIYLT
                     PAAAARAGRLVALSEFLASLNPTDVHSLRFLCEIATSRGLFDEAKKYAGSLFDAQPES
                     YQAACRWLKFELDDRQTAPARIDFLDSVTHNTKYSAAFRSHAETLLSGLYVAQGDTAR
                     ASEAISHALQLDPTSDEALLGRLALTQNPSNELKAVTMLEMLRGNPRNWSVSRDMAVL
                     LNEIGLPELSAKYYSLAWAIASNGLAEEKMSQELAIGYCNALLSDSKPDKVISLLSKR
                     FKDFKDTPAVMELYVEALKTSGAAASAILPILEQMKNAYKSQINQIGIRSNIDPIMDK
                     GRKTSPELADAIIQLGIFYLTTAANPELAEGEFDRARQFGAKGDVIDMLQAAAAITIK
                     KDDPSAVKTLTKLSTDFAYANLSLAEYYAKTGEKENEKKFLSQGLKMTRGTRVSRALR
                     AMWSFTTPIPKIDDVDKVAAAVNGFDAQVLYMATNPNDFIKVTASAIGAPIVSPGQAM
                     NVAVTVTNIGQLPVSFGETGVLSSQVALSVVTAGKDGLRFYTAPVVILPVDRYLAPGK
                     SVSGDVRLDTAQISAYLANDPLTTIQLDIKPCLNPVWDAKGYVQSLLPMIEPGRMTFY
                     RAGLLATGKLSQSGSPLVNISPEWYTRVLGAMNDTLAGDNVSDRFRTAKQIAAMLAYS
                     RAIEGELAKPLPELRGVFRVEPIMALAEKIVQDKSPAVRAEFVASMNNISTITPNIIN
                     LIGPIFQDSSDIVRLRVAEFLGNVGGKGNMEILHMFAADKNPYVSTMAKAFLFYEKNR
                     QEARSSAGGQPRQ"
     gene            complement(3863..>4441)
                     /locus_tag="PLK08_06865"
     CDS             complement(3863..>4441)
                     /locus_tag="PLK08_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPS53256.1"
                     /translation="TPAVMVDQIIPSGQAAAMGLANRDLIIAVNGQPLQAFATPELAA
                     MNFTKWLMSRTVGEKVRFTVLQGSQKKDIELTLLPMPKMPSEADKLYVKKLGLLMREK
                     VALDRHMDDSPAADAQGLLVLSVVPNSPADRAGLKAGDVVVGINRKEVPTAADGKKAI
                     EAAISNKPPLDLLMIVQRGADRLRLTIETPDN"
BASE COUNT          900 a         1122 c         1225 g         1184 t
ORIGIN      
        1 tctgccgccg tttttctgcc gccgggctgc aaaattatta cctattacag gcagccgagg
       61 tttttgggat aggttctata tataattcaa cgtcgtccca gaaatattcg cctggcggcc
      121 aatatgagta tatctgaatc tgcaaaaatt ggacgttatc gggcagtctg tcgcgcggag
      181 gaaatttgcc gtcaaacgtt ttccaggctg acgtcacttc gcagttcata tgccatcgat
      241 aacattctcg cagataattt tgcggattcc ggcgggcatc tgcttcgatg agttcccgct
      301 gtttcatcgg cggcaggttg gcgaaatccg caggggtcat tttgagtttt ttcaatgtgc
      361 tttcgggcag gccgttgatg ttggctaatg gtatttttcg aaaacctttt ataaaaactt
      421 tcgacgtggc agacgcacgc ggattagacg gctgaatgaa acggcaggat gcttttagcc
      481 agtatgttgc gtttgtgtcg gctggaatga aatcgctttt gaaatgtacg ccttcctggc
      541 cggcgatggt gtcgtttttt cccgatgtga tttgcggcgg ggagtcgacg gatgcttcgc
      601 cagccgacaa tgcttttcgg taagccagcc attgttccct gtcgacatcc gttcgaatca
      661 gcaacatttt tccgctgtta tcaggacgct cgacgataaa actggcgaca ttgtccggag
      721 attcccaacc gccgtggccg gcgagctcaa aagacgggtt tgcgtttatc ggaccggtaa
      781 gccggtctgc cgacggaaat tgttcatcgc ctgcctgtgg cggttgttct gtctgtctgc
      841 cgataaaggc ctgtatgatt ttttgtgcga tgtttccacg ccagcgttcc gtttcatccg
      901 aaaaagtgtt tttgacgacg gtttgcgggt tagtgagggt attgaaaatg cgaacatctg
      961 cgacatattt attgccagcc ttttttannn nnnnnnncga atatgcaggc atgtgcggct
     1021 acctgttctt gcagcagcgt ccggagcgac atggccgttg aattcatgtc aggcgatgat
     1081 atggcaagct cccgcattga ttgcgtatct atgactttcc agcggccatc ctggcgggcg
     1141 agtttaagcc gcactgcatc ggcaagtgca gcggcgtctt ccgactgcgg cgagaagtca
     1201 aacaccgcca caaagacatc gccatcgccg gtctcaccaa acgtacgcac tgctgcaaag
     1261 catatcagcc ataataatac tgctacggcg gctgttcgga atgttccgga ttgtgctcgc
     1321 tttatggagt tcattgattt attggcgcgg ctgtccgccg gctgatgagc gggcttcctg
     1381 cctgtttttc tcataaaaca gaaatgcctt tgccattgta ctgacatatg gatttttgtc
     1441 ggctgcgaac atgtggagaa tttccatatt gcctttgccg ccgacgttgc ccagaaactc
     1501 cgccactctc aatctcacaa tgtcgctgga atcctggaaa atcgggccga ttaaattgat
     1561 tatgttcggc gttatggtgg aaatattgtt catggatgcg acgaattcgg cacggacggc
     1621 aggcgacttg tcctggacga ttttttccgc cagagccatt atcggttcga ctctgaatac
     1681 gcctcgcagt tcaggcagcg gcttggccag ttcgccttcg attgccctgg aatacgccag
     1741 cattgccgca atctgttttg ccgtgcggaa tctgtcgctg acgttatcgc cggctaacgt
     1801 gtcgttcatt gcaccgagta cgcgcgtata ccactccggc gatatattga caagcggact
     1861 gccggactgg gaaagtttac cggtggccag caggccggca cggtagaagg tcattcggcc
     1921 gggttctatc atcggcagga ggctttgaac gtaacctttg gcgtcccata cgggattgag
     1981 gcagggcttg atgtcgagct gtatggtcgt aagcggatcg ttcgccaggt atgccgatat
     2041 ctgtgcggta tccagccgta catcgccgga aacgcttttg ccaggcgcca gatatctgtc
     2101 taccggcaga atcactaccg gtgcggtata aaaacgcaag ccgtccttgc cggctgtaac
     2161 cacgctcagt gctacctgcg aactcaacac gccggtttcg ccgaaggaga ccggcagttg
     2221 gccgatgttg gtaacggtga cggcgacgtt catcgcctgg ccaggcgaaa ctatcggagc
     2281 accaatcgca gatgccgtga cttttatgaa atcattggga ttggtcgcca tgtagaggac
     2341 ctgtgcgtcg aagccgttga cggcggctgc aaccttgtcg acgtcgtcga ttttgggtat
     2401 cggtgtcgtg aagctccaca tggcacgcaa tgcgcggctg actcttgtgc cgcgggtcat
     2461 tttgaggcct tgcgaaagga atttcttttc attttccttt tcgccggttt tcgcgtaata
     2521 ctccgccaac gacagattgg cgtaagcgaa atccgtcgac agttttgtga gagtttttac
     2581 ggctgacggg tcgtctttct ttatggttat ggcggcggca gcctggagca tgtctatcac
     2641 gtcgcccttt gcgccgaact gtctggcccg gtcgaattcg ccttcagcca gttccggatt
     2701 ggcggctgtc gtgaggtaga aaattcccag ttgtattatc gcatcagcca attcaggcga
     2761 tgtttttctg cccttgtcca taatagggtc aatgttcgaa cgtatgccta tctggtttat
     2821 ttgcgatttg tacgcgtttt tcatctgttc cagaattggt aatatcgcag aagctgcggc
     2881 gccggatgtt ttgagggctt cgacgtaaag ttccatgacc gcgggcgtat ccttaaaatc
     2941 tttgaatcgc ttcgagagca gagaaataac cttgtccggt ttggagtcag acagcagcgc
     3001 gttgcaataa cctattgcca gctcctgcga cattttttcc tcagccaagc cgtttgacgc
     3061 gattgcccat gccaggctgt aatattttgc cgacagttcc ggcaggccga tttcgttgag
     3121 cagaaccgcc atatcacggc tgacgctcca gttgcgagga ttgccgcgca gcatttcgag
     3181 cattgtgaca gccttgagtt cattcgatgg attctgcgtc aaggcaagac ggcctaacag
     3241 ggcttcgtcg cttgtcgggt cgagctgtaa tgcgtgcgat atcgcttcag atgccctggc
     3301 ggtatcgccc tgtgcgacat agaggccgga aagcagagtc tctgcatgcg accgaaaagc
     3361 ggcggaatat ttggtgttgt gagtgacgga gtcgagaaaa tcaatcctcg ccggcgcggt
     3421 ctgacggtcg tcaagttcaa atttcagcca tcggcaggcg gcttgataag attccggctg
     3481 ggcgtcgaag aggctgccgg cgtatttttt cgcttcgtcg aacaggcctc tggacgtagc
     3541 gatttcgcac aggaatcgca acgagtggac gtcggtggga ttaaggcttg ccaaaaattc
     3601 gcttagtgcg accagccgtc cagcccttgc ggctgccgcc ggagtaagat atatactctg
     3661 tgcgtcggcg agtatggaat tgatttcaag cgttttttct acgcctgcat ctgctgcgaa
     3721 aaccaggctc gacgtcgcaa gaatcatcat gcccagaacg gctgcctttt tcacgatgcg
     3781 ctccttttgc attgttattt ttgttatttt gttatttttt aaaaaattct gttgtcgcaa
     3841 atccgtttac agccatgaga gattaattgt cgggcgtttc aatggtcagc cgcagacggt
     3901 cggcgccgcg ctggacaatc atcaacaggt cgagcggcgg tttgtttgaa atggcggctt
     3961 cgatagcctt tttgccgtcg gcggcggtgg ggacttcttt gcggttaatg cccaccacga
     4021 catcgccggc tttaaggccg gcacggtcgg ctggcgaatt cggcactacg gagagtacca
     4081 gcaagccctg ggcatcggcg gctggactgt cgtccatgtg ccggtcaagt gctacctttt
     4141 cccgcattag caggcccagt ttcttgacgt aaagtttgtc ggcttcactg ggcattttgg
     4201 gcatgggcag cagggtgagt tctatatcct ttttctgcga accctgcaaa acagtaaacc
     4261 tgaccttctc gccgacggtt ctgctcatca gccacttggt gaaattcatc gccgccagtt
     4321 ccggcgtggc aaacgcctgc agcggctggc cgttgaccgc tattatcagg tcgcgattgg
     4381 ccaaacccat tgccgcagcc tggccggacg gaattatctg atcaaccata acagccggcg
     4441 t
//
LOCUS       DAONFI010000193         4439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_23484_length_4439_cov_4.397126, whole genome shotgun sequence.
ACCESSION   DAONFI010000193 DAONFI010000000
VERSION     DAONFI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23484_length_4439_cov_4.397126"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PLK08_06870"
     CDS             complement(<1..1186)
                     /locus_tag="PLK08_06870"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HPS53257.1"
                     /translation="MKPKSRIKSQFDLFRANFNQILNLDHELCQLAKAIDWPKFESGF
                     ASCYSSDMGAPAKAVRLMVGMHYLKYMFNESDESVVEHWVENPYWQFFCGYEYMQHKC
                     PIHPTTMVKWRQRVGAERLETMLTETLEMAVRGGHVTTHQFSKVTADTTVQEKAVAHL
                     TDARLYQKSRCRLVKLAVKRGIKLRQSYERVGKYEFIKQSRYANAQQFRRAAKCTRKL
                     KTYLGRVIRDIERNAGTMDDELSTILKRARRIHQQSREDKNKLYSLDAPEVSCIAKGK
                     AHKKFEFGCKISLVVSNVGDWILGVRRVEGNRHDSKTLAAAIKQAERISGVKVGEIFA
                     DKGYRGWDYEGDAKVHIVGTSNRDLPRSTRKRKKRRSAIEPKIGHLKSDHRMNRNYLK
                     HAA"
     gene            complement(1380..2537)
                     /locus_tag="PLK08_06875"
     CDS             complement(1380..2537)
                     /locus_tag="PLK08_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53258.1"
                     /translation="MPKNLAISEKLFEKLRAEYLRQWGITLTCTSPAGDMVCGKVPCS
                     REGESFACRSLRAQAIEETLRWGEPTVVYCEDRHLLWAMPIMHNEVVIGGLLACTTER
                     KVLTIRKDGSLFNLRGACESLRIMCERENLTNASALAIAREKYQAEQRRAYAIHDYKN
                     VSHSNLRELYLREEPELFSAIRCGDRRSAQEILNRVLVGIYFHADNRFEVVKSFVLEL
                     IVSMCRTAVDAGGEPEELLGANFAVMTALAKIKDEEELTAWLTQTFERLFSSIQKSRA
                     SDSNSVLMDAVAFMERHCGENVSRNDAARAAHLSPQYFSSLLHQKYGLTFTELIGRMR
                     IDRAAEMLANTNNPLSVIAIDCGFQDQSYFTKVFKKYRKTTPLEYRKTAIK"
     gene            2670..4049
                     /locus_tag="PLK08_06880"
     CDS             2670..4049
                     /locus_tag="PLK08_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HPS53259.1"
                     /translation="MKYRKLAIDNVDDLLFGVSPHPLTTRHGMVLGGGQVYPELNFTL
                     PPMIIDKSTWPKATEHYRNIIHGALHRAAELYSPGVQIECELLPPMTQNPEWGGEISR
                     ILVEAMEEAHAKHGLKSVLRMTPNDTREMVRPPVMRSGQLWEDMLDTFRRCAATGAEL
                     LSIESVGGKEVHDEALTMADIRGVIFALCIMGVRDMRFLWEHIVKIAKEYNVYAAGDT
                     ACGFGNTAMVMAEQKLIPRVFAAIVRPITAVRSLVAYECGAVGPGKDCGYENPILKAI
                     TGYPMAMEGKTAACAHLSPVGNIAAACCDVWSNESVQNIKLLGAMAPTCYMEQLEYDC
                     RLMNQASADGHDGAKTLQKWLVDSDAYLDPQAYVLTPESTIAFAKAIVESPNYYAAGK
                     NVALTAVARIRESFAAGDLKLEDREIPFLDIVESTVESMPDKEDDFIAEIMPELDKSK
                     FVAADYGLA"
     gene            complement(4159..>4439)
                     /locus_tag="PLK08_06885"
     CDS             complement(4159..>4439)
                     /locus_tag="PLK08_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPS53260.1"
                     /translation="RRGVQGGYFLNCDPAEITVGKVIRIVEGPLDPVRCTGDKESADC
                     PMKGKCSLIRLWDKARLAVEHVYDSTTFKDLVEQDKNLKNNHIQNYSI"
BASE COUNT         1056 a         1171 c         1110 g         1102 t
ORIGIN      
        1 cggctgcatg cttaagatag ttcctgttca ttcgatggtc gctcttgaga tggcctatct
       61 tcggctcaat cgcacttcgc cgctttttgc gtttccgcgt cgaacgtggc aggtccctgt
      121 tgctcgtgcc gacaatatga actttcgcat cgccttcgta atcccaaccg cgataaccct
      181 tgtccgcaaa aatttcgcca accttcacgc cgctgatacg ctcggcctgc tttatcgcag
      241 ccgccagcgt cttgctgtcg tggcgattgc cctcaacacg ccgaacgccc aaaatccaat
      301 caccgacatt gctcaccacc aacgatatct tgcagccgaa ctcgaacttt ttatgtgcct
      361 tgccttttgc aatgcacgac acctccggcg cgtcgaggct ataaagcttg ttcttatctt
      421 cgcgggactg ctgatgaatc cgcctggccc gtttgagaat ggtcgataac tcgtcgtcca
      481 tcgttccggc gttgcgttcg atatcacgaa tgaccctgcc gagatacgtt ttgagttttc
      541 gcgtgcactt tgcggcacgc ctgaattgtt gagcgttggc gtagcggctt tgcttgatga
      601 actcatattt gcccacacgt tcgtaactct gccgaagttt tatcccgcgt tttaccgcaa
      661 gcttcacgag ccgacagcgg gacttctggt acagcctcgc atctgtcaga tgtgcgaccg
      721 ctttttcctg aaccgtcgtg tccgccgtaa ccttcgagaa ctgatgcgta gtaacatgcc
      781 cgccgcgaac agccatctcc aacgtctccg tcaacatcgt ctcaagccgc tccgctccca
      841 cacgctgccg ccatttcacc atcgtcgtcg gatgtatcgg acatttgtgt tgcatgtact
      901 cgtaaccgca aaaaaactgc cagtacggat tttcaaccca gtgttcgaca accgattcgt
      961 ccgactcgtt gaacatgtac ttcaaatagt gcatgccgac catcaaacgc accgccttgg
     1021 ccggcgcgcc catgtccgac gaataacagc tggcaaagcc cgactcaaac ttcggccaat
     1081 ctatcgcctt tgcaagctgg cagagttcgt ggtcgaggtt gagaatctgg ttgaagttgg
     1141 cacggaagag gtcgaactga cttttgatgc gggatttcgg cttcattttg cggttccatt
     1201 ttgcaaggtt tcgggctttc atccatgaaa accttgcaaa ttgtaaacca tattctcaca
     1261 tcgtccagcc tttattcacc gaagaaaaac tattttttca gggacgacta atgatttatc
     1321 gttgtttata gtaaaaacaa tttttgccga tcaattcctg aattcacgaa ccggatagtt
     1381 cactttatcg cggttttacg atattccagc ggcgtagttt tgcggtactt tttgaaaact
     1441 ttcgtgaagt agctctgatc ctgaaatcca cagtctattg ctatgaccga gagcggatta
     1501 ttcgtgttgg ctagcatttc ggctgcccgg tcaatgcgca tgcggcctat cagttccgta
     1561 aacgtcaggc cgtatttctg gtggagcagc gatgaaaaat attgcggcga caaatgggcc
     1621 gccctcgccg cgtcgttgcg agaaacattt tcaccgcagt gacgctccat aaacgcaact
     1681 gcgtccatga gcacagaatt ggaatcgctc gcacgtgatt tctggatgga actgaatagc
     1741 cgctcaaaag tctgcgtcag ccacgcggtc aattcttcct catcttttat tttcgccaac
     1801 gcggtcataa cggcgaaatt cgcgccgagc agttcttcgg gttcgccgcc ggcatcgacg
     1861 gctgtcctac acatgctgac aataagctcg agcacgaaac ttttcaccac ctcgaacctg
     1921 ttgtcggcgt gaaaataaat tcccaccaga acacggttga gaatttcctg cgcggaacgc
     1981 ctgtcaccgc aacggatagc tgaaaacagt tccggctctt cgcggagata tagctcacgc
     2041 agattggagt gggacacgtt tttgtaatcg tgaatggcat atgcacgccg ctgctcggcc
     2101 tgatattttt ctcgcgcgat agccagtgct gatgcgttgg taagattctc cctttcgcac
     2161 atgatgcgca gtgattcgca cgcgccgcga agattaaaca gagaaccgtc tttgcggatg
     2221 gtcagaactt tgcgttcagt cgtacacgcc agaagtccac cgattacgac ttcattatgc
     2281 attattggca tagcccagag cagatgtctg tcttcgcaat aaaccacagt aggttcaccc
     2341 cagcggagag tttcttctat tgcctgtgcc cgcaggctgc gacacgcgaa agattctcct
     2401 tcgcgggagc aagggacttt tccgcaaacc atatcaccgg cggggcttgt gcaggtcaac
     2461 gttattcccc attggcgaag atattcggca cgcagttttt caaataattt ctcggagatt
     2521 gcaaggtttt tcggcattaa gcatcctttt ttattatctt accattttac caaaaatagt
     2581 cgcagcttca caagacgctt tttttgattt atgcgacaat atttccagtg ttgaatgctg
     2641 acggaaaact tttacttcag gagaatgata tgaaatacag gaaactggca attgataatg
     2701 tggatgacct gctctttggg gtttcaccgc atccgctgac cacaaggcac ggcatggtac
     2761 ttgggggcgg acaggtttac cctgagctga attttacgct tccacctatg attattgata
     2821 aatcaacatg gccgaaagct accgagcatt acaggaacat catacatggc gcgttgcatc
     2881 gcgcggcaga gctgtattcg ccaggcgtgc agattgaatg cgaacttctt ccgcctatga
     2941 cgcagaatcc cgaatggggc ggtgagatat cgcggatact cgtggaagcg atggaagaag
     3001 cgcatgcgaa gcacggcctg aaaagcgtac tccgtatgac accaaatgat acgcgtgaaa
     3061 tggtacgccc gccggtaatg cgcagcggcc aactgtggga agacatgctc gatacgttca
     3121 ggcggtgtgc cgccaccggc gcggaactgc tgagtatcga atctgtcggc ggcaaagaag
     3181 tacacgatga agcactcaca atggctgaca ttcgcggggt tattttcgcg ttgtgtatca
     3241 tgggcgtgcg cgatatgcgt ttcctttggg agcacatcgt caaaatcgca aaggaataca
     3301 acgtgtacgc agccggcgac actgcctgcg ggtttggaaa caccgccatg gtgatggctg
     3361 agcagaagct cataccccgc gtgttcgcgg cgatagtgag gcctataacg gctgtacgct
     3421 cactggttgc gtatgaatgc ggcgcagtcg gccctggcaa ggactgcggg tacgaaaacc
     3481 cgatactcaa agccattacg ggatacccga tggccatgga gggcaagacg gccgcctgcg
     3541 cgcatctcag cccggtgggg aatatcgctg cggcgtgttg tgacgtctgg agcaacgaat
     3601 ccgtgcagaa catcaagctg ctcggcgcga tggccccgac gtgttacatg gaacagctcg
     3661 aatacgactg ccgcctgatg aatcaagcca gcgcggacgg tcatgacgga gcgaagacgc
     3721 ttcaaaaatg gctggtcgat tcggacgcgt accttgaccc gcaggcgtat gtgcttacgc
     3781 cggaaagcac catagcgttc gccaaagcga tagtcgaatc tccgaattat tacgccgccg
     3841 gtaaaaatgt ggcgttgacg gcggttgcac gaatccgcga atcattcgca gccggcgatc
     3901 tgaagctgga agatagggaa atcccgttcc tggacatcgt ggaaagcacc gttgagtcca
     3961 tgccggacaa agaggacgat tttattgcgg aaatcatgcc agaactggac aaatccaagt
     4021 tcgtagctgc tgattacggt ttggcgtaaa atccttgacg caacgacatg aaatggtggg
     4081 atgattttca gactagagga aatcatcctg ccattttcat gtgtgaaaaa agatagagaa
     4141 tggaaacagc cgacgatttc agattgaata attctgaatg tgattgtttt tgagattttt
     4201 atcctgctct accaaatctt taaaagtcgt actgtcgtaa acgtgttcaa cggcaagccg
     4261 agccttatcc cacagacgaa tcagcgaaca cttacctttc atgggacagt cggccgattc
     4321 cttatctccg gtacaacgca ccgggtcaag cggaccttca acaatgcgta taaccttgcc
     4381 gacagtaatt tcagcggggt cgcaattcaa aaaatatcca ccctgcaccc ctcgccgtg
//
LOCUS       DAONFI010000194         4436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_23509_length_4436_cov_4.225748, whole genome shotgun sequence.
ACCESSION   DAONFI010000194 DAONFI010000000
VERSION     DAONFI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23509_length_4436_cov_4.225748"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..939)
                     /locus_tag="PLK08_06890"
     CDS             complement(64..939)
                     /locus_tag="PLK08_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPS53261.1"
                     /translation="MHVPDSMLHGNVCPVTAFFSTVGVLGAAWLAFRRKVQASAGRFA
                     AVTSLIFAAQMVNFPVSGGTSGHLLGGVVAAALLGMPMGVLAMTLVIVIQSLLFADGG
                     LSVMGVNIMSMAIIGAGLGGGLFELLSMGAAIRSRRGMAALAVASWLSVVLASSFVSA
                     MLVIDGASKGNPGPSGIGVIISQDGSVIKEIGKYIGTATNNIAEYTALLHALQEGLIL
                     KADSIRIKSDSELLCRQLNKQYKVKSPNIIDLYTQAVHLLTGFKEVSIQHIPREQNAH
                     ADKLATQAVKDALKG"
     gene            complement(1124..2107)
                     /gene="galE"
                     /locus_tag="PLK08_06895"
     CDS             complement(1124..2107)
                     /gene="galE"
                     /locus_tag="PLK08_06895"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPS53262.1"
                     /translation="MNVLLTGGAGYIGSHVVRALQLAGHRCVVYDNLSKGHRQAVDGM
                     ELVVGDVCDSAALRDTFKKFEIEAVINLAAFIEAGESVEKPKKYFENNTLAGFTLLNA
                     MRDCGVNRLVFSSTAAVYGTPERTPILETDRLAPINPYGASKLCVEYMMQAYARAYGM
                     GFVALRYFNVAGAHPSGQIGEDHSPETHLLPLILQVASGKREKILIFGDDYDTPDGTC
                     IRDYIHVCDLADAHVLAVDAVEQGQVRIFNLGNGEGFSVNEVIETCRRVTGKPIAAQN
                     AQRRPGDPDRLVASSCKAVSQLNWKPKFPHIETIVEHAWKWHSSHPDGYGK"
     gene            2395..4356
                     /locus_tag="PLK08_06900"
     CDS             2395..4356
                     /locus_tag="PLK08_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPS53263.1"
                     /translation="MLPKKVLIALCVLFMVTLVFYGSAICQADTSDNSASLENILLAA
                     KDKGGDEFSEFEDLEEKGEKSTEKTDETAVEKTETVVEVKETELDKQWQDMLYYITVA
                     RPEYAKAYANAILKNADASPKNIYMLSVKYSDSIKTLQLGEGLGSMKELVEKLLKTIE
                     TGYGDWRTNAEQIEESISKLQGTQRAFKIATARLAESGEYAVPLLIVKLQSSETSKLV
                     IERIIAILPQMGKSAVRPLSAALQSNDLKLVEIIADALGQMQYPAALPRLCEAYARND
                     VKENKNVLRAVKGAILACSGGDSSLLDMNPGRVFLKVADMYYSKSSSLLPDSKKDAAL
                     VWSMKKQGTAEALTAEPVPAVIFCDVYAMRYARLALKYDRTLSQAIPLWLSAAIRRQL
                     ELPAGAKDPLWAQNAPKAEFYALASSPRYLQDVLAKALDDNNTGIAKVVISAMNRNTG
                     TDSLIMPVAGGKMPLVAAMNSDDKDVRYLAAETLALAQPKKSFTGQSSVVEILKEALK
                     EKGEWSIRAAAAFQAIGGGTTCFDLTGCVESLKAALAGGSAELQKAIVNALAVMNDSQ
                     AQQTIVDFALTGKTEEESRIAAFNAATRSVRAFGNQTSSQQAKNLAAMLIMVEGSQNL
                     RQSAAQLLGALNLKSSQMSNLILSTEKLD"
BASE COUNT         1195 a         1174 c         1107 g          960 t
ORIGIN      
        1 ctgccctcag gagcccggac tttcctcccc ggacgcttgc gcatccggag taaccacccc
       61 cacttatccc tttaaagcat cctttaccgc ttgtgtagcc aatttatctg catgcgcgtt
      121 ttgttcgcgc gggatatgct ggatcgatac ctctttgaaa ccggtcaaaa gatgcaccgc
      181 ctgtgtatag aggtcgatga tattcggact tttaacctta tactgtttat taagctggcg
      241 gcacagaagc tcgctgtcgg atttgatcct gatgctgtcc gctttgagga tcaatccttc
      301 ctgcaacgcg tgcaaaagcg ccgtgtattc ggctatatta ttggtcgcgg tccctatgta
      361 tttgccgatc tctttgataa cggagccgtc ttgtgaaata atgacaccga tgccgctggg
      421 acccggattt cccttcgatg cgccgtcaat aacgagcatg gcacttacga acgaactcgc
      481 cagcacaacc gacagccacg atgcgacagc taacgccgcc atgccgcgtc tggaacggat
      541 ggccgcaccc atcgaaagca gttcgaacag cccgccgccg aggcctgcac ctattatcgc
      601 catgctcatg atatttacgc ccataacgga caagccgccg tcggcaaaaa gcagcgactg
      661 gatgactatc accagcgtca tggccaacac gcccatcggc atacccagca gagccgccgc
      721 aactaccccg ccaagaagat gtcccgaagt tccgccgctt acgggaaagt tgaccatctg
      781 agccgcaaat atcaaagacg tgacggctgc aaaccggccg gctgacgcct gaaccttgcg
      841 acgaaacgcc agccaggctg cccccaatac gccaaccgta ctgaaaaacg ccgtaacagg
      901 acatacattt ccatgaagca tcgagtcagg cacatgcatt ttgtttctcc ttaaaaaaac
      961 gcaataaccg taactgtaaa gtttaattca atatgcggcg aaaatccaag aggaaatcac
     1021 aaatcctgaa aaagacaatc ggtagccgac ttttccgtaa aaaaatcatc tgctaccagc
     1081 cgccgcatgc caaatagtca tttatgcgga cgtgcacaaa atttcatttt ccgtatccgt
     1141 ccggatgcga gctgtgccat ttccaggcgt gttcgactat cgtttcaatg tgcgggaatt
     1201 ttggtttcca gttcagttgc gatacagcct tacagctgga ggcgaccaat ctgtcagggt
     1261 cgccaggccg tcgctgggca ttttgggcag ctatgggttt acccgtcacc ctgcggcagg
     1321 tttctatcac ttcgttaacc gaaaaacctt cgccgttgcc gaggttgaat atcctgactt
     1381 gcccctgttc cacggcatca acggctaaca cgtgggcgtc ggcaaggtcg cagacgtgga
     1441 tgtaatcgcg tatgcatgtg ccgtccggcg tatcgtaatc gtcgccgaaa ataaggattt
     1501 tttcgcgttt gcccgatgca acctgcagaa ttagcggcaa gagatgcgtc tcaggggagt
     1561 gatcttcgcc gatttggccg gatggatgtg cgccggcaac gttgaaatat cgcaatgcga
     1621 caaaccccat gccgtacgcc ctggcgtatg cctgcatcat gtattccacg cataatttgc
     1681 tggcgccgta ggggtttatc ggggcaagac ggtcggtttc gagtatcggc gtgcgttcag
     1741 gagtgccata tacggctgcc gtcgatgaaa ataccagcct gttcacgccg cagtcccgca
     1801 tggcgttgag cagcgtaaag ccggctaacg tgttgttttc gaaatatttt ttgggctttt
     1861 cgacggattc gccggcttcg atgaacgccg cgagattgat gaccgcttct atttcaaatt
     1921 ttttaaaagt gtcccgcaag gcggctgagt cgcatacgtc gcccacgaca agttccatgc
     1981 cgtcgacagc ctgccggtgg cccttgctca gattgtcgta aaccacgcac cgatggccag
     2041 ccagttgaag agccctgacc acgtgcgagc ctatatagcc ggctccgcct gtcagcaaaa
     2101 cattcattta aaacgcactc ccgttaaaaa aaactctcaa aaaaacgccg acgaattata
     2161 aacgccgtcg caatgaaatg caaaaaaaag caaaaccgaa aatgctcgca tgaaggatat
     2221 caaaatatca tcacaaagaa cacgggtatt atgttgacag aaagtctgca atttctataa
     2281 ttacaatgac attatggact tttgttatgt cctcaggctg taagcgtgcg ttttgtcgac
     2341 gcacaaggac cccgaatccg gcatgccgtc aggccgttta gcaggagtat taatatgttg
     2401 cctaaaaaag ttctgattgc cctgtgtgta ttgtttatgg ttacgctcgt attttacggc
     2461 tcggcaatat gtcaggccga cacttctgac aactccgcat ctctcgaaaa catcctgctg
     2521 gcggcaaagg acaaaggcgg ggatgaattt tccgaatttg aagacctgga agaaaaaggc
     2581 gaaaaatcca cagaaaaaac cgacgaaaca gcagttgaaa aaacagaaac tgtagtggaa
     2641 gtcaaagaaa ccgaactgga caagcagtgg caggatatgc tttattatat caccgtcgcc
     2701 cgtcctgaat atgccaaagc ctacgccaac gcaatactca aaaatgccga cgcctcgcct
     2761 aaaaacatct atatgctctc ggtcaaatat tccgactcga taaaaacgct gcaattaggc
     2821 gaaggcctcg gcagcatgaa ggaactcgtc gaaaaactgc tgaaaaccat agaaaccggc
     2881 tacggcgact ggcgaaccaa cgcggagcag atagaagagt ccatttcgaa actccagggc
     2941 acgcagcgcg cattcaagat tgccaccgcc cgccttgccg aaagcggcga atacgcagtg
     3001 ccgctgctta tcgtaaaact tcagagcagt gaaacgtcca aactggttat tgagcgaatc
     3061 attgccatcc tgccgcagat gggcaaatca gccgtcaggc cgttatcggc ggcattgcag
     3121 tcaaatgacc tcaaactggt ggaaatcatc gcagacgcac tcggccagat gcagtatccg
     3181 gcggcattgc cgcggctttg cgaagcatat gcacgaaacg acgtaaaaga aaataaaaac
     3241 gttctgcgag ccgtcaaagg tgcaatatta gcctgctcag gaggcgacag cagcctgctg
     3301 gacatgaacc cgggcagggt ttttctaaaa gtagccgaca tgtactactc aaaatcgtcc
     3361 agcctgctgc ccgactcaaa aaaagatgcc gcactggtgt ggtcgatgaa aaaacaggga
     3421 acggcagagg cacttactgc cgaacctgtg ccggctgtta tattctgcga cgtttacgcc
     3481 atgcgttatg cgcggctggc gttgaaatat gacagaacac tcagccaggc gataccgctg
     3541 tggctgtcgg ctgcaatacg cagacagctt gaactgccgg ctggtgcgaa agacccgttg
     3601 tgggctcaaa acgcaccaaa agcggaattt tacgcattag ccagcagccc gcgatatctg
     3661 caggatgtat tagcaaaagc actggacgac aacaatacag gaattgccaa agtcgtaatt
     3721 tccgcaatga atcgcaatac cggcacggac agccttataa tgccggtggc aggcggcaaa
     3781 atgccgctag tggcggcaat gaatagcgac gataaggacg tacgctatct ggccgcggaa
     3841 acgctggctc ttgcacagcc gaaaaaatcc tttaccggcc agtccagcgt agtcgaaata
     3901 ctcaaagaag cactgaagga aaaaggcgaa tggtcaattc gtgcagcggc tgcattccag
     3961 gctatcggcg gcggaacgac atgtttcgac ctgaccggtt gcgtggagtc gctcaaggct
     4021 gcgttagccg gcggcagtgc ggaactccag aaggcaatcg taaatgcgct agccgtcatg
     4081 aacgattcgc aggctcaaca gacaatagtc gacttcgcac taaccggcaa gactgaagaa
     4141 gaatcacgta tagcagcctt caacgcagct acgcgttccg tgcgggcatt cggaaatcag
     4201 acatcatcac agcaggcgaa aaatctggct gctatgttga taatggtgga aggttcgcaa
     4261 aatcttcgtc agtcggcagc ccagctcctc ggagcgctta acctgaagag ttcgcaaatg
     4321 tccaatctca tactcagtac ggaaaaactc gactgagtaa aactattgga taagcagtct
     4381 tttaagagcg tctaacaaaa tgagatgtcg caccgaggcg tagttggttg attggc
//
LOCUS       DAONFI010000195         4359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_24064_length_4359_cov_4.952602, whole genome shotgun sequence.
ACCESSION   DAONFI010000195 DAONFI010000000
VERSION     DAONFI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24064_length_4359_cov_4.952602"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(527..1870)
                     /locus_tag="PLK08_06905"
     CDS             complement(527..1870)
                     /locus_tag="PLK08_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPS53264.1"
                     /translation="MNIETMIIPAILIFYAVVVVAIGFLDSRHVKGFDDYVFAGRNRG
                     LLMVTSSTLASIVGASATIGMVNLAYASGFPSFWWLGSGAVGLVAGSLLVAKNIYNLK
                     AYTLADIVDKLLGPWTRRAVSVLIIAAWAGIVAAQYVAAGQLISLLCGLPYLAAMLLT
                     AGFITIYCAMGGQASVLKTDFIQLLFIVAGLGIALWALYGKTGLPAGGLKFELVNEEF
                     PASMVWYMMLIVGSSFVIGPDIFGRLFTARSATVARRSMFISGLLLTVVSFGIVMIGL
                     WAKDFAAIPSGERNNVLPWLMMNAMSKPLGVVLAMGLLSALISSADTCTITAATTFEH
                     DLRGGSSVAGTRMATIAVSIVAVCLAWKNPGIIHTLLVAYSIFNCGVFPALLFAIVFY
                     RKVRLNPVFATAGIIAGGVLGGVSGMTQRPGLAIYAMAGSAIFALFALYKGRRVG"
     gene            complement(1867..2346)
                     /gene="tadA"
                     /locus_tag="PLK08_06910"
     CDS             complement(1867..2346)
                     /gene="tadA"
                     /locus_tag="PLK08_06910"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPS53265.1"
                     /translation="MNLSDAEKYERLMRLALEQAKEALKYDEVPIGAVIERNGEIIAS
                     AFNRRIIDADPTGHAEMIAIRQAAGKLGDWRLEGCTLAVTLEPCCMCAGAIVLARIPT
                     LVYGAADAKGGAVDTLYNICNDGRLNHSVQVIGGVLADDAGRLLSDFFRRQREMGKK"
     gene            2571..3125
                     /locus_tag="PLK08_06915"
     CDS             2571..3125
                     /locus_tag="PLK08_06915"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPS53266.1"
                     /translation="MKEFPLAKVFHLLEPGPTVLVTTASKESGSNIMTMSWHMMMEFQ
                     PPLIACVVSPCNYSYAALLSTKQCVIAIPTVELAATVVGIGNCDAPDVNKFEKFSLTP
                     LPAKKVKAPLIAECIANIECKVTDTRMANKYAMFILTAVQAWFDPKHKEHRMIHHNGD
                     GTFEVDDKKTLNLRRKMTKWKDIS"
     gene            complement(3168..4274)
                     /locus_tag="PLK08_06920"
     CDS             complement(3168..4274)
                     /locus_tag="PLK08_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS53267.1"
                     /translation="MTSRERLLTVLDNGRPDRLPCQVHSWMQYYLDTYLNGMNQYEAY
                     EFFEGMDQVVYVTAKSIFKEADLDKWRIERIETPADADGVTHYAEIISTPGGKLTHQF
                     ASNRFTTWTTEYLIKTERDWELADRYIPVPCGCDQSAIIEARNRIGEKGIVRGLYFGF
                     GQGSPWQDLCCLMDTQQAIYAACDNPQWIHAMLESILQKRLRFWNVAGKLECDLLETG
                     GGAGSSTVISPNMHSEFCLPYDIRQHSAVKSISPVKIVYHLCGGLMPLLNIVTKNGAD
                     GLETMTPPSMGGDCDLKKASAQIGDKMFFVGGFDQNAGFERGTPQVVKKLVRECFEAT
                     KDNAGYICSPSDHFFKGDVENIKTFARECSECRY"
BASE COUNT         1175 a         1216 c          962 g         1006 t
ORIGIN      
        1 acgtcaaccg taatttaacg gttttaaaaa caattcaaaa cacgtcaacc gtaatataac
       61 agtttttaaa gcaatttaaa atacatcaac cgcagttcaa cgctttttaa aacaactcaa
      121 atataccacc cgcaatttaa aaaatttaaa aaccattttg cgcaaaatgg ccacgatttg
      181 caatattctt ttgccttgat aacgataaga actatttggt ttatagtgaa tctctacggg
      241 ttaaaacccg tagcatcata ttcatcaggc tttcgcctgt cagcctcacg gcggatactt
      301 tcatctacgg tttaaaaacc gtagaattac gaaggccata ttacattgga aattattttg
      361 taaaaaaatt gataaatcca atcggcaagt gaaaaatgta aatgatacag cttttgggac
      421 acagccccct atattcgaat cggtttcccc cagccggttc gattttaagg cggtgcttgc
      481 agttctcccc ccactgcagg cccgccgttt ttttgtgaat cgttctctac cctacccgac
      541 ggcctttata cagtgcaaag agagcaaaaa tcgccgagcc ggccatggca taaatggcaa
      601 ggccaggccg ctgcgtcata ccggaaacac cgccaagaac accgcctgca attatgccgg
      661 ctgtcgcaaa aacgggattc agtctgacct tgcgataaaa aactatcgcg aataacagcg
      721 ccggaaacac cccgcaatta aaaatactat acgccacaag cagcgtatga ataatgccag
      781 gatttttcca cgccaggcag accgcgacaa tactcactgc aatagtagcc atccgtgtgc
      841 ccgctacgct actgccgcct ctcaggtcat gttcaaatgt tgtagccgca gttatcgtac
      901 acgtatccgc cgacgatatc agtgcagaaa gcaagcccat ggcaagaacc actcccagcg
      961 gcttggacat ggcattcatc atcagccacg gcagaacgtt atttcgctcg cccgacggta
     1021 tggccgcaaa gtctttcgcc caaaggccta tcatcactat accgaaactt acgaccgtca
     1081 gcagcaggcc ggaaatgaac atcgaccgcc tggctaccgt ggctgacctg gcagtaaaaa
     1141 gacggccgaa aatatcgggg ccgatgacaa acgaactgcc aacgatgagc atcatatacc
     1201 acaccatcga cgccggaaat tcttcgttga ccaattcgaa tttcagtccg ccagcaggca
     1261 ggccggtttt cccatacaat gcccaaagtg caatgccaag gcccgccacg atgaacagca
     1321 actgtataaa atccgtcttc agtaccgacg cctggccgcc catcgcacaa tatatcgtaa
     1381 taaaacccgc agtcagcaac atcgcagcca gatacggcag gccgcagaga agtgaaataa
     1441 gctggccggc ggctacatac tgcgccgcga caatgccggc ccacgccgca attatcagca
     1501 cagacacagc ccgacgagtc caggggccaa gcagcttgtc gactatatcc gccagcgtat
     1561 aggctttgag gttatagata ttcttcgcca cgagcaggct tcccgccacc aagcccaccg
     1621 cgccgctgcc aagccaccag aatgaaggaa aaccgctggc atacgccaga ttcaccatgc
     1681 ctattgtcgc cgacgccccg actatgctgg ccagcgtcga tgacgtaacc atcaaaaggc
     1741 cgcgattccg gccggcaaac acgtaatcat caaatccttt tacatgacgt gaatcaagaa
     1801 aacctatcgc cacgacaacg acggcataaa atatgagaat tgcagggatt atcatcgttt
     1861 caatgttcat tttttaccca tctcacgctg acggcgaaaa aagtcgctca ataatcggcc
     1921 ggcatcgtca gccaatacac cgccgatgac ctgtacgcta tgattgagtc tcccgtcgtt
     1981 acagatgttg tacagagtgt cgactgcgcc gcctttggcg tcagctgcgc cgtacacgag
     2041 cgtcgggatt cgggcaagca ctattgcgcc tgcgcacatg cagcatggtt cgagcgtcac
     2101 cgccagcgta cagccttcca gccgccagtc gccgagtttt ccggcggctt gacgaatcgc
     2161 aatcatttcc gcatggccgg tcgggtcggc atcgattatc cgacgattaa aagcggaagc
     2221 tattatttcg ccgttacgtt cgattaccgc gcctatcgga acttcatcgt atttcaacgc
     2281 ttctttcgcc tgttccagcg caagccgcat caaacgctca tatttttctg cgtcggacaa
     2341 attcatcgcg aagattgtgt gctttttccc gacaaatgtc aattgacttc cgtctgcagt
     2401 tacaaatgta acgcctcgcg tggatatcgc atgcccgcct gcatataatt ttaaaaacaa
     2461 tccgcaaaaa catgggcgtc gccgaaatca agcaattttg ctggctggcg acatcagaaa
     2521 aatgcggcat atttttttaa aaaaaacgta aatgaacaag gagtgatacc atgaaagaat
     2581 ttccgctggc aaaagttttt catcttctcg aaccagggcc gacggttctc gtaacgactg
     2641 cgtcaaagga gagcggcagc aatatcatga cgatgagctg gcacatgatg atggaattcc
     2701 agccgccgct cattgcctgc gtagtcagcc cgtgcaatta cagttacgcc gcgctgcttt
     2761 caacaaaaca gtgcgtaatc gccatcccca cggtagagtt agccgccacc gttgtcggca
     2821 tcgggaattg cgatgcgccg gacgtcaaca agttcgagaa attcagttta accccactgc
     2881 cagccaaaaa ggtcaaagcc ccacttatcg cagaatgcat agccaacatc gaatgtaaag
     2941 tcacggacac acgcatggcc aataaatacg ccatgttcat actgactgcg gtacaggcgt
     3001 ggtttgaccc aaaacacaag gaacaccgca tgattcatca caacggtgac ggcacgttcg
     3061 aagtcgatga taaaaaaacg cttaacctgc gaagaaaaat gaccaaatgg aaagatattt
     3121 cgtagaacct atcccaaaaa catgggcctc gcctggaaca tgctcttcta atacctgcat
     3181 tcagagcact cgcgggcaaa tgtttttatg ttttctacgt cgcccttgaa aaaatggtcg
     3241 cttggactgc agatataacc cgcattatct ttcgtagctt caaaacactc gcggacaagt
     3301 tttttaacaa cctgcggcgt acctctttca aaaccggcgt tctggtcgaa tccgccgaca
     3361 aagaacatct tgtcgccgat ttgagccgac gcttttttca agtcgcagtc gccgcccata
     3421 ctcggcggcg tcatggtttc caagccgtcg gctccgttct tcgtgacaat attcagcagc
     3481 ggcatcaggc cgccgcaaag atgataaact atttttaccg gcgatatgct cttaacagcc
     3541 gagtgttgcc gtatatcgta cggcaggcag aattcggagt gcatattcgg gcttatcacg
     3601 gtgctagacc cggctccgcc gccggtttcg agaaggtcac attcaagttt gcctgcaacg
     3661 ttccagaatc tgagccgctt ctgcaatatg gattccagca tcgcatgtat ccactgcgga
     3721 ttgtcacacg cagcatatat ggcctgctgc gtatccatca gacaacacaa atcctgccac
     3781 gggctacctt gcccgaatcc gaaatacaac ccgcgaacaa tgcctttttc gccgatgcga
     3841 ttccgcgcct cgattatcgc cgactggtca cagccgcacg gcacggggat atacctgtcc
     3901 gccagctccc aatcgcgctc ggtctttatc aggtattccg tagtccaggt cgtaaacctg
     3961 ttcgacgcaa attgatgcgt cagtttgccg cctggcgtag agattatttc tgcataatgc
     4021 gttacgccgt cggcatcggc tggagtttct atgcgttcaa tccgccattt atcaaggtca
     4081 gcctctttga atatggattt ggcggttacg tatacgacct gatccatgcc ctcgaaaaat
     4141 tcatacgctt cgtactgatt catgccgttc agataagtat cgagataata ctgcatccag
     4201 ctatgtacct ggcagggcag ccggtcaggc cgcccgttat cgagaaccgt caatagtctt
     4261 tctcgcgaag tcatgttgca ttctcctgta accgatgtcc agttttagaa cctatcccaa
     4321 aaacatgggc gtcgccgaaa tcgagcaatt ttgctggct
//
LOCUS       DAONFI010000196         4354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_24095_length_4354_cov_4.176087, whole genome shotgun sequence.
ACCESSION   DAONFI010000196 DAONFI010000000
VERSION     DAONFI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24095_length_4354_cov_4.176087"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="PLK08_06925"
     CDS             <1..1383
                     /locus_tag="PLK08_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53268.1"
                     /translation="IPDFSFPYRIGPFASLYRWRFPWESYKQLFDERLGYSGFGPYEA
                     LLRDVLNNFGQGGWFWSSRWTGNISKTSRFAFHLRTLSKIKLAMMFKRGRGDSNPAQL
                     PVQYSDEWITDYDAAVEYYNKEQKEFSDKLSEYQQALKDAQQNNTTAPDAPVLNSKIM
                     TTRYYDVIVKSTIPWDSPDWMNPSAYDASTPTNTFVPERWFSWQLPSGKGPYTKASAY
                     PLHRWIRDLKGWRPMTYTSYNSDTSPSAWTEGRWYEVASRVWAKKWKTQASYDRYDQL
                     NLPIRYQDPDNPTSGTIPYTIYYFQIRVFGGIELRNEIMVSDPLDGASPDDLPAPILL
                     DSDKCEYNSTGEQANHSSYLRFLGVASRSAPARVWPGQFTLGNPAGRMTAVAEAVVFN
                     NSSWDLWTQDWRAQLVPVSGWEDWQNTLATQLDGLNDVDTQENPDDVRQTLEYLLSLD
                     PQFMDLYLDH"
     gene            1385..2107
                     /locus_tag="PLK08_06930"
     CDS             1385..2107
                     /locus_tag="PLK08_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPS53269.1"
                     /translation="MQCARNIFGPKTSRRGTAMIETVFCIPLFALLLLGVFFLGWAMR
                     CEQKLIIASRYAAWRTAANREVYADAWKTAWINYGEPDEEESRNQFVDDYETRNKIEP
                     TAAYLNERFFTGTGSDTTISTGGWPNDVLEEYVSDARNEDTDAGDLADRTALGDFPSG
                     SGAKVASQFEHDYDLYLRFTHGDTVMDRSHYIDATSWRRWQTSLQEPMRVLFLNEWDE
                     TVKTVEPQALQTSLEEMYSQDW"
     gene            complement(2147..4180)
                     /locus_tag="PLK08_06935"
     CDS             complement(2147..4180)
                     /locus_tag="PLK08_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPS53270.1"
                     /translation="MYKLRFRQVHLDFHTSPDIPDVGSKFDKKQFQEALKIGHINSIT
                     CFSKCHHGWSYHDTKVGRRHPNMQGELFRRQYEACKEIDVNIPIYLSAGVDNVITMTH
                     PEWREINTDARFAGWFTSPLMPGFHKMCFNTPYLEYLCEQIREAVTMFPEVDGVFLDI
                     ISQGQCCCNWCMAKYRELGLDPTKEENRKIVAEIGLERYYEMTTAAAKCNNPDMPVFH
                     NSGHIRRGRRDLFKWFSHLELESLPTGGWGYDHFPLSAKYCKNLGNLDFLGMTGKFHT
                     TWGEFGGYKHPNALRYECAAMLAFGSKCSVGDQLPPLGQADKSTYELIGHAYSEVEQK
                     EPWCDDVTNIADVGLLSSEACAGVVLDRCHPSQADTGANRILLEGHFLYDILDVDMDF
                     NKYKMLILPDDISIKSAVKTKLDAYLKNGGKLLMTGKSGLDDDGYAMFDIGATVDGLS
                     EYQPDYILPIKKLRASFVDSPLVMYVQSRRLKVTAGESLGDVYDPYFNRTWEHFCGHQ
                     HAPYQPHASGYDCGVVNGNIMYLPHPVFTLYCGMGAVAYKEFLCNCIRRLLGSDESIC
                     VNLPSTARVSLMRQCGQNRYVLHLLYANTVNRGGEMPLAGGTTTADTTKSVEVIEDLT
                     PLYNTTVLLKIPEHIKTVTLEPQGKELPFKKDGRTVSFTLDEFTCHQMVVLHY"
     gene            complement(4119..>4354)
                     /locus_tag="PLK08_06940"
     CDS             complement(4119..>4354)
                     /locus_tag="PLK08_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53271.1"
                     /translation="YDGREFRSAMGRQAEIHPECRQAATVAAPYRRKINNDYLTCSEI
                     RRFYGIDERRMPFYVQIAIQTGSSGFSYFAGYT"
BASE COUNT         1074 a         1153 c         1115 g         1012 t
ORIGIN      
        1 attccggatt tcagttttcc gtacagaatc gggccgttcg cgtcgttata ccgttggcgg
       61 tttccgtggg aaagttataa acagctcttc gatgaacggt tgggatattc aggtttcggc
      121 ccatatgagg cgttgttgcg ggacgtgttg aacaatttcg gccaaggcgg ctggttctgg
      181 agcagcagat ggactggcaa tataagcaag acgagccgtt ttgcttttca tctccgcacg
      241 ctttcaaaaa tcaaactggc catgatgttc aaaagaggcc gcggagattc caatcctgcc
      301 cagctccccg tgcaatacag cgacgaatgg ataaccgact acgatgccgc ggttgaatat
      361 tataacaagg agcaaaaaga attttccgac aagctcagtg agtatcagca ggctctcaag
      421 gatgcccagc aaaacaatac taccgctccg gatgcgcctg tactcaacag taaaatcatg
      481 accaccagat attacgacgt aatcgtgaag tccaccattc cctgggacag tcccgactgg
      541 atgaatccat cggcatacga cgcgtctacg ccgacaaaca cgttcgtgcc ggaaagatgg
      601 ttcagctggc agttgccgtc cggcaaaggc ccatatacga aagcctccgc ttatccgctt
      661 catcgctgga taagagacct caagggctgg agaccgatga cttatacttc gtacaattcg
      721 gacacatcgc catcggcgtg gacggaaggc cgctggtatg aagtcgccag ccgcgtctgg
      781 gcgaaaaaat ggaagaccca ggcgtcatat gaccgctacg accagttgaa tctgccgatt
      841 cgatatcagg accctgataa tccaacgtcc ggtacgattc catatacgat atattacttt
      901 cagatacgcg ttttcggcgg aattgagttg aggaacgaga ttatggtttc cgacccgctc
      961 gacggcgcca gtccggacga tttgccggcg ccgattctgc tcgacagcga taaatgcgaa
     1021 tacaattcca ccggcgaaca ggctaatcac agttcgtacc tgcgtttttt aggcgtcgcc
     1081 agccgcagtg cgccggctcg cgtctggccg ggccagttca cgctcggcaa tccagccggc
     1141 cgaatgacgg ctgtggcgga agccgtggta ttcaataatt caagctggga tttgtggacg
     1201 caggactggc gagcccaact tgtgccggtc agcggctggg aagattggca aaatacccta
     1261 gccacccaac ttgacggcct gaatgacgtc gatacgcagg aaaaccccga tgacgtccgg
     1321 caaacgctgg agtacctgct gtcgcttgac ccgcaattca tggatttata cctggaccat
     1381 tgatatgcaa tgcgcaagaa acatattcgg gcccaaaact tctcgccgcg gcacagcgat
     1441 gatagagacg gtattctgca taccgctttt tgcattgctg ctgctgggcg ttttttttct
     1501 cggctgggca atgcgctgcg agcagaaact cataatcgcg tcacgctacg ccgcgtggcg
     1561 gacagccgcc aatcgcgaag tttatgcaga cgcatggaaa acggcctgga taaactacgg
     1621 cgaaccggac gaagaagaat cgcgaaacca gttcgtcgac gattatgaaa cacgcaataa
     1681 aatcgagccg acggcagctt atctgaatga gcgttttttc accggcaccg gcagcgacac
     1741 gacgatatca accggcggct ggccgaacga cgtgcttgaa gaatacgtca gcgacgcacg
     1801 caatgaagac accgacgcag gcgacctcgc cgaccgtacg gcactcggcg attttccgag
     1861 cggtagcggc gcaaaagtcg cgtcacaatt tgagcacgat tacgaccttt atttgcggtt
     1921 cacgcacggc gataccgtaa tggacaggtc acattacatt gacgcgacaa gctggagacg
     1981 atggcagacc tcgctgcagg agccgatgcg ggtccttttt ctaaatgaat gggacgaaac
     2041 tgtaaaaacg gttgagccgc aagctctcca gaccagcctc gaagaaatgt attcacaaga
     2101 ctggtgatta gaacctatcc caaaaacagc ccaatatcaa tcactatcaa taatgaagaa
     2161 caaccatctg gtggcaggtg aattcgtcga gcgtaaagct gactgtccgg ccgtccttct
     2221 tgaacggcag ttctttgccc tgcggttcaa gagtaacggt ttttatatgt tccgggattt
     2281 tcagcaacac cgtcgtattg taaagcggcg tgaggtcttc aatgacttcg acgctttttg
     2341 tagtgtcggc tgtcgtcgtt ccgccggcga gcggcatttc tccgccgcga ttgacggtgt
     2401 tggcgtaaag caggtgcagg acgtagcggt tctgtccgca ttgacgcatg agggacacac
     2461 gcgcggttga aggcagattc acacaaatgc tttcgtcgct gccgagcagc cggcgaatgc
     2521 agttgcacaa aaattcctta tatgccaccg cacccatccc gcaatatagc gtaaataccg
     2581 gatgcggcag atacatgata ttgccattga cgacgccgca gtcataaccg ctggcatgcg
     2641 gctgatacgg cgcatgctga tggccgcaga aatgttccca cgtgcgattg aaataggggt
     2701 cgtagacgtc gccaagcgac tcgcccgccg tcactttcag ccgccgcgat tgcacgtaca
     2761 tcaccagcgg agaatccaca aaagacgcac ggagtttttt tatcggcaga atataatccg
     2821 gctgatattc gctcaagcca tcaaccgttg caccgatatc aaacatggca tagccgtcgt
     2881 catccaggcc gctttttccc gtcatcaaca gtttgccgcc atttttgaga tacgcatcca
     2941 atttcgtttt gacggctgac tttatactta tgtcgtcagg cagtatcagc attttatatt
     3001 tattgaagtc catgtcgacg tcgagtatgt cgtagaggaa atggccttcc aataaaatgc
     3061 gattcgctcc agtatcagcc tggctgggat ggcagcgatc caaaacaacg ccggcacacg
     3121 cctcgctcga aagcaggccg acatcggcga tattcgtcac gtcgtcgcac cagggctctt
     3181 tctgttccac ttcgctgtag gcgtggccga taagttcata ggtgcttttg tcggcctgcc
     3241 ccagcggcgg cagctgatcg cccacactgc attttgaacc gaacgccagc atcgctgcac
     3301 actcgtaccg cagcgcattt ggatgtttgt agccgccgaa ttcaccccat gttgtatgaa
     3361 acttgcccgt catacccaaa aaatccagat tgcccagatt cttgcaatac tttgccgaca
     3421 gcggaaaatg atcgtatccc catccgccgg tcggcagact ttccagttca agatggctga
     3481 accatttgaa aagatcgcgg cggccacgac ggatatggcc ggagttgtga aaaaccggca
     3541 tatcgggatt attgcatttc gccgcggcgg tagtcatttc ataatatcgt tccaggccga
     3601 tttcagctac gattttgcgg ttttcctctt ttgtcgggtc gaggccaagt tcacggtatt
     3661 tggccatgca ccagttacag cagcattggc cttgagagat gatatccaga aaaacgccgt
     3721 cgacttctgg aaacatggta acggcttcac gtatctgttc gcagagatat tccaggtacg
     3781 gcgtattgaa gcacatttta tgaaaacccg gcatgagcgg actcgtaaac cagccggcga
     3841 atctggcgtc cgtatttatt tcccgccatt caggatgcgt catggttatt acgttatcca
     3901 cgccggcgga gagatatatc ggaatattga cgtctatttc cttgcaggct tcatactgcc
     3961 gacgaaaaag ttcgccctgc atgttcggat gccgccggcc gactttcgtg tcgtgataac
     4021 tccagccgtg atggcatttc gaaaagcagg taatcgaatt gatatgcccg attttcaacg
     4081 cttcctgaaa ctgctttttg tcgaattttg agcccacgtc aggtatatcc ggcgaagtat
     4141 gaaaatccag atgaacctgt ctgaatcgca atttgtacat aaaaaggcat cctccgctca
     4201 tcaatgccgt aaaaacggcg aatttcagag catgttaaat aatcattatt tattttacgc
     4261 ctataaggtg cagcaacggt tgcggcctgt cgacattcgg gatgaatttc ggcctggcgg
     4321 cccatggcgc tacggaattc acgcccgtca tatc
//
LOCUS       DAONFI010000197         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_24220_length_4340_cov_4.648308, whole genome shotgun sequence.
ACCESSION   DAONFI010000197 DAONFI010000000
VERSION     DAONFI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24220_length_4340_cov_4.648308"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="rfaE2"
                     /locus_tag="PLK08_06945"
     CDS             <1..1041
                     /gene="rfaE2"
                     /locus_tag="PLK08_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPS53272.1"
                     /translation="DSLKSVDVLAVEDYGKGLFCDQLSDVIAAARKESIHVIVDPHPT
                     ADYRRYQGCSLLTPNRTEAEIASGVKITSDETMNLAADAILSITHADAVVITLDREGA
                     FIKRRDGFAAKVPTRQRKVYDVTGAGDVVMAMLSMAMACNCELHKAVALCNVAGGLEV
                     ERFGVTPVTRHEIIDEIRKMMGCRREKILDRKSLKQQVEYLHSQGKSVVFTNGCFDLL
                     HVGHITYLRESRELGDCLVVAVNSDASVQRLKGPLRPIISQQDRATMLASLEFVDYVT
                     IFDEDTPEAVLEALRPDVLIKGGSTPVIIGQKFVESYGGSVRRLDLVEGFSTTDIVKK
                     ILELNGKEVERD"
     gene            1041..2123
                     /locus_tag="PLK08_06950"
     CDS             1041..2123
                     /locus_tag="PLK08_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPS53273.1"
                     /translation="MTYRAVFLDRDDTVIEDAIYLSDPDGIKLLPGVGLAIKSLRQAG
                     YKIVVVTNQSGIARGLITEDALERIHSHLRQMLADQGAMLDAIYYCPFHPEGTVEKYR
                     RDSDLRKPGPGMLIKASEQLNIDLKNSWMVGDSSRDIAAGQAAGCRTIRLQPRDTGKP
                     FNNQADSHPADFVARNLVEAAKIILNSHKTETAAPTAPIFTRQQNGKPAELPKAPQPA
                     SQPTPTPQPQPTQKKTAALANETEILSQNSEKTADSDCPPNLPNAGGTKMNNANQHDD
                     SMIRREILQHIRQLTKQHEHDEFSLLNLFGGLTQVLTLLFLFLVFWSMLSVEADANYE
                     KAIVWGIVGLIFQCMSLTFYTMTKKK"
     gene            2138..3415
                     /locus_tag="PLK08_06955"
     CDS             2138..3415
                     /locus_tag="PLK08_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPS53274.1"
                     /translation="MKIKAFRGWHYAEKNVSNLIAPPYDILDKSDKDALLAQSPNNIV
                     AVDLPHMPPKELGPQQAYLDSAALLKQWQTAGILVQDAKPAIYAYEQSYNWAGKTYAR
                     RAILVGIKASPFGQEIIPHEHTFAGPKADRLRLTECTKAQLSPIFGFYDDSRGATAAL
                     WRTASRKPDIQGELNGITEKLWIIDDEAVIKNLAAALDNVPIYIADGHHRCTTAMNYA
                     DALKAAGKIGPDDEANYILFALVAKDDPGLLVLPTHRLINGLSREFTVEKLCAELGGA
                     FECQNIGCVCNVNLADADAVLAKSGKHAFGIIFNGEFYVLKFVDPQAMKAAAPAHCQA
                     WRELDVAILQELILDRAMEKFKAADYVVEYTPDGELAKKKVASGQTQVAFCLQGTPLK
                     AVEDVANAGESMPHKSTYFYPKLATGMIIKPLE"
     gene            complement(3461..4120)
                     /gene="plsY"
                     /locus_tag="PLK08_06960"
     CDS             complement(3461..4120)
                     /gene="plsY"
                     /locus_tag="PLK08_06960"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPS53275.1"
                     /translation="MNPWLYALPLAAYLIGSTPFGVIIARSKGVDLRKCGSGNVGATN
                     VGRTMGRKWGMLCFTLDALKGFIPTLLAGYMLGVLHRVPTTAEQLLWLGVAAGCIFGH
                     VWSFWLMGKGGKGVATSLGVVLGFFPYFTYAGLCGFALWIIVTKISRYVSLGSVCSAV
                     VFTPLFIIFNIHRDMAGLWLLGAFAILMSALIIYMHRGNIKRLIAGTENKIGTRKTGN
                     P"
     gene            complement(4117..>4340)
                     /locus_tag="PLK08_06965"
     CDS             complement(4117..>4340)
                     /locus_tag="PLK08_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53276.1"
                     /translation="RYNGASEVLLDGAGVVIGRPDDIDAVVAGLNRLVDPEERSRFVN
                     ACAAAGEKLTWRRHCSELLEIYAKQMEKK"
BASE COUNT         1128 a         1243 c         1115 g          854 t
ORIGIN      
        1 gacagcctca agtcggttga cgttttagcc gtcgaagatt acggcaaggg tcttttttgc
       61 gaccagcttt cggacgtcat agcggctgcc cgcaaggaaa gcattcacgt aattgtcgat
      121 cctcatccca cggcggatta tcggcgatat cagggctgtt cgctgcttac gcccaatcgg
      181 acggaagcgg agattgcaag cggcgtaaaa ataacatccg acgaaaccat gaacctggca
      241 gccgacgcga tacttagtat cacgcatgct gacgctgttg taatcacgct tgacagggaa
      301 ggcgctttca taaaaaggcg agacggcttc gcagccaaag tgcccacgcg gcagcgaaag
      361 gtttatgacg taactggtgc aggcgatgtg gtgatggcca tgctgtcgat ggcgatggcc
      421 tgtaactgcg aactacacaa agccgtcgcc ctctgcaacg tcgccggcgg ccttgaggtc
      481 gagcgtttcg gagtcacgcc tgtcacgcga catgaaatca tagacgaaat acgcaagatg
      541 atgggctgcc gtcgtgaaaa gattctggac aggaaatcac ttaaacagca ggtggaatat
      601 cttcacagcc agggcaaatc cgtcgtattc accaacggct gtttcgacct cctgcacgtg
      661 ggccatatca cctatctgcg cgagtcgcgt gagcttggcg actgcctcgt ggtagccgtc
      721 aattccgacg ccagcgtaca gcggctgaaa ggccccctac ggcccataat ttcgcaacag
      781 gacagggcga ccatgctcgc ctcgctggaa tttgtggatt acgtgacgat attcgacgaa
      841 gacacgcccg aagcagtgct tgaagcgctt cggccggacg tgctaataaa gggcggttca
      901 acacctgtaa taatcggcca gaaattcgtg gagagctacg gcggcagcgt caggcggttg
      961 gacctggtgg aaggcttcag caccacggac atcgttaaaa agatattgga actcaacggc
     1021 aaagaagtgg agcgggactg atgacataca gagctgtctt tttggacaga gacgacacag
     1081 ttatagagga cgcgatatat ctcagtgatc cggacggcat aaaacttctc cctggcgtag
     1141 ggttggcgat aaaatctctc cggcaggccg ggtacaagat tgtagtcgtt accaaccagt
     1201 ccggaatagc tcgcggcctc ataacggagg acgctctgga gcgtatacat tcgcatctgc
     1261 ggcagatgct cgccgaccag ggcgcgatgc tggacgcgat ttactattgt ccgtttcatc
     1321 ccgaaggcac agtggagaaa taccggcgtg attccgatct tcgcaagccg gggcccggca
     1381 tgctaataaa ggcatctgaa cagctcaata tagatctgaa aaattcgtgg atggtgggcg
     1441 acagttcccg cgacatagca gccggccaag ccgccggctg cagaaccatc cgcctgcagc
     1501 cgcgagatac aggcaaaccg ttcaataatc aggctgacag ccatccagcc gacttcgtgg
     1561 caagaaatct cgttgaggcg gcaaaaataa ttctgaactc gcataaaacg gaaaccgccg
     1621 cgccaactgc gcctattttc acgcggcagc agaatggcaa gccggctgaa ctgcccaaag
     1681 caccacagcc agccagccaa ccgaccccaa ctccgcagcc gcagccgaca cagaaaaaaa
     1741 cggcggcctt agcaaacgag acggaaattc tatcccaaaa cagtgaaaaa acagccgaca
     1801 gcgattgtcc gccgaacctt ccgaacgccg gaggaacaaa aatgaacaac gccaatcagc
     1861 atgacgacag catgatcaga cgtgaaatat tgcagcacat ccgccagctt accaaacagc
     1921 acgagcatga cgaattcagc cttctgaatc tcttcggcgg actgacgcag gttctgacgt
     1981 tattgttcct gttcctcgtg ttctggtcga tgttgagcgt ggaggctgac gccaattacg
     2041 aaaaagccat cgtatggggc atcgtaggcc ttatattcca gtgcatgtcc ctgacctttt
     2101 acaccatgac aaaaaagaag taggagaatt ttcagccatg aaaatcaagg cattccgcgg
     2161 gtggcattac gcagaaaaaa acgtttcaaa cctcattgcc ccgccttacg acattctgga
     2221 caaatccgat aaggacgcac tgctagccca aagccccaac aacatagtcg ccgtcgatct
     2281 tccgcatatg ccgccgaagg aactcggccc tcagcaggca taccttgact ccgccgcact
     2341 cctgaagcaa tggcagaccg ccggcatact agtgcaggac gccaagcctg ctatctacgc
     2401 atatgaacag agctacaact gggcaggcaa aacatatgcc cgccgggcaa tactcgtcgg
     2461 catcaaggcc agcccgttcg gccaggaaat cataccgcat gagcacacct ttgccggccc
     2521 gaaagccgac cggctcaggc tcaccgaatg cacaaaagcg cagctgtcgc cgatattcgg
     2581 cttctatgac gattcccgcg gcgcaacagc cgccctgtgg cgaaccgcca gccgcaaacc
     2641 cgacatccag ggcgaactca acggcataac ggaaaagctc tggattatcg acgatgaagc
     2701 cgtaataaaa aacctcgcag ccgcccttga caatgtcccg atatatatcg cagacggcca
     2761 tcatcgttgc actacggcta tgaactacgc cgacgcgcta aaagccgccg gcaaaatcgg
     2821 gcctgatgac gaagcgaatt atattttatt cgcactcgtc gcaaaagacg acccaggcct
     2881 gctggttctg ccgacgcatc gactcatcaa tggccttagc cgcgaattca ccgtcgaaaa
     2941 actctgcgcc gaactcggag gcgctttcga gtgtcaaaat atcggctgcg tgtgcaacgt
     3001 gaatcttgcc gacgcagacg ccgtgctggc caaatccggc aagcatgctt tcggaataat
     3061 ttttaacggc gaattctacg tattgaaatt cgttgacccg caagccatga aagcagccgc
     3121 accggctcac tgccaggcat ggcgggaact cgacgtcgcc atacttcagg aactcatcct
     3181 tgacagggcg atggaaaaat tcaaggccgc cgattacgta gtcgaatata cccctgacgg
     3241 cgaacttgca aaaaagaagg tagcatccgg ccagacgcag gtggcattct gtttgcaggg
     3301 cacgccgcta aaagccgtcg aagacgtcgc caatgccggc gaatcaatgc cgcacaagag
     3361 tacctacttc taccccaaac tcgcaaccgg catgattatc aagccgctgg aataacaaaa
     3421 tcagagtgtt tccacaaaaa aatttccttc gagagttttt ttacggattg cctgtttttc
     3481 gcgtaccgat tttgttttcc gtgccggcga taagtctttt tatattgccg cgatgcatat
     3541 aaattatcag tgcgctcatc agaattgcaa acgcgccgag cagccagagg cctgccatgt
     3601 cgcgatggat gttgaatatt ataaacagag gcgtgaaaac gacggcagag catacgctgc
     3661 ccagtgagac gtagcggctg atttttgtca cgattatcca aagtgcgaac ccacataggc
     3721 cggcatacgt aaaatagggg aaaaatccca ataccacgcc gagagaagtg gcgacaccct
     3781 tgccgccttt tcccatcagc cagaagctcc agacatgccc gaatatgcag cctgccgcca
     3841 cacccagcca gagcagctgt tccgccgtcg tcggcacacg atgcagcaca ccaagcatat
     3901 accccgccag cagcgtcggt atgaaccctt tgagtgcgtc gagcgtaaag cataacatgc
     3961 cccacttgcg gcccatcgtc cggcctacgt tggtcgcccc cacgttgccg ctgccgcact
     4021 tacgaaggtc gacgcccttt gaacgcgcga tgattactcc gaacggcgta gagcctatca
     4081 ggtatgccgc cagcggcagt gcgtaaagcc agggattcat tttttctcca tttgtttggc
     4141 atatatttcc agcaactcgc tacagtggcg tcgccatgtg agtttttcgc cagccgccgc
     4201 gcaagcgttg acaaaacggc tgcgttcttc ggggtcgaca agcctgttca atcccgcgac
     4261 tacggcgtca atatcgtcgg gccgtccaat caccactcct gcgccgtcga gcagaacttc
     4321 cgacgcaccg ttatatcgcg
//
LOCUS       DAONFI010000198         4240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_25005_length_4240_cov_5.154361, whole genome shotgun sequence.
ACCESSION   DAONFI010000198 DAONFI010000000
VERSION     DAONFI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25005_length_4240_cov_5.154361"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLK08_06970"
     CDS             <1..979
                     /locus_tag="PLK08_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPS53277.1"
                     /translation="LPRVTEIFEARKPKDPAAMSEISGRVELRTDKRRGKMTVIVRNE
                     KTSMEREHHVPRDRHLLVHTGDYVEAGEPLTEGPMVPHDILEICGEEILQTYLLGEVQ
                     NVYRSQGVTINDKHIEIILGQMLRKVQVENVGDSTLLPGDVIDKHRFNEENRRLASSL
                     KIVDPGDSGLVEGDIVARERVEQANAEAEATGGEPAKTRKPKPATARTLLLGITKASL
                     QSESFLSAASFQESTKVFTEASLAGKVDDLRGLKENVILGHLIPAGTSFKPYLEMKLK
                     KSVEENLFETAKNDNINDISAAEMDAFAKQAAVIIDDEADDDDDFGDEE"
     gene            1087..1458
                     /gene="rpsL"
                     /locus_tag="PLK08_06975"
     CDS             1087..1458
                     /gene="rpsL"
                     /locus_tag="PLK08_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPS53278.1"
                     /translation="MPTINQLIRKPRKVQPAKSKNPALDQCPQRRGVCLLVKTVTPKK
                     PNSALRKVARVRLSNGKEVNAYIPGIDHNLQEHSIVLVRGGRVRDLPGVRYHIIRGTL
                     DTLGVNGRKQARSRYGAKKPK"
     gene            1731..2207
                     /gene="rpsG"
                     /locus_tag="PLK08_06980"
     CDS             1731..2207
                     /gene="rpsG"
                     /locus_tag="PLK08_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPS53279.1"
                     /translation="MGRKKYTASAKTLQPDPKFGSKLLAKFINILMEDGKKYAAQEIM
                     YKALDIVAKKVKDDTPLVVFEKAIDNLKPRIEVRSKRVGGANYQVPMPVNSRRQQSLA
                     MRWLRDAIRGGKGRATYLKTADEIISAYKGEGTAITTRENVHRMAEANKAFAHFAW"
     gene            complement(2566..3978)
                     /gene="ahcY"
                     /locus_tag="PLK08_06985"
     CDS             complement(2566..3978)
                     /gene="ahcY"
                     /locus_tag="PLK08_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPS53280.1"
                     /translation="MSKDYKVADIKLSDSGRKEITIAEYEMPGLMATREKYGKDKPLA
                     GVKIMGSLHMTIQTAVLIETLTALGADVRWCSCNIFSTQDQAAAAIAAAGIPVFAWKG
                     ETLEDYWNCTQQAMTWPDGSGPDQIVDDGGDATLLIHRGYAAENNAAILDEPTKSKEL
                     QIINALLKKMLKHDPQHWHKVAKNVRGVSEETTTGVHRLMQMEERGELLFQAINVNDS
                     VTKSKFDNIYGCRHSLVDGISRAMDVMLSGKVAMVCGYGDVGKGSAESLAGQKCRVMV
                     SEIDPICALQACMAGYQVCTVEDALPLADIYVTTTGNTDILTANHMSRMKDQAIVCNI
                     GHFDNEIQVYELENFPGIKKEEIKDSHCPVHRFTFPDGHCIYLLAEGRLVNLGCATGH
                     PSFVMSNSFTNQTLAQIDIHKNPDRKIDVYRLDKKLDEEVARLHLDKLGAKLTRLTDA
                     QAKYIGVPVEGPYKPEHYRY"
     gene            complement(4043..>4240)
                     /locus_tag="PLK08_06990"
     CDS             complement(4043..>4240)
                     /locus_tag="PLK08_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53281.1"
                     /translation="KSGSSVSLLGPDDGITVTNMTAAVSANADAPTKTLLVKVTLNMT
                     DLSGNDISMNISASPRKNLIN"
BASE COUNT         1044 a         1019 c         1135 g         1042 t
ORIGIN      
        1 cttgccgcgc gttacggaaa tattcgaagc acgcaagccc aaagatcctg ctgcaatgtc
       61 ggaaatatcc ggccgcgttg aacttcgtac cgacaaacgc cgcggcaaaa tgaccgttat
      121 cgttcgaaac gaaaaaacca gcatggaacg cgagcaccat gtgccgcgcg accgtcacct
      181 cctggtacac accggcgact acgtggaagc cggcgagccg ctgaccgaag gacctatggt
      241 gccgcacgac attttggaaa tctgcggcga agaaattttg cagacgtatc tgctgggcga
      301 agttcagaac gtttaccgca gccagggcgt tacaatcaac gacaaacaca tagagataat
      361 cctcggccag atgctccgca aggtgcaggt ggaaaatgtg ggcgattcga cacttttgcc
      421 cggagatgtt attgacaaac accggttcaa tgaagaaaat aggcgactcg cttcgtcgct
      481 taagattgtt gaccccggcg atagcggcct tgtggaaggc gatatcgttg cccgtgaacg
      541 cgtagagcag gctaacgccg aagccgaggc gaccggcggc gaaccggcta agaccaggaa
      601 gccaaagccc gcaacagcca ggacgctatt acttggcatt accaaggcgt ctctgcagtc
      661 ggagagcttc ctgtcggcgg ccagcttcca ggaaagcacc aaggtgttta cggaagcgtc
      721 gttagccggc aaagtcgacg acttgcgagg cctcaaggaa aacgtgatac tcggccacct
      781 gataccggcc ggcacaagtt tcaagccgta tcttgagatg aagctcaaga agagcgtcga
      841 agaaaacctg ttcgaaacgg caaagaatga caatataaac gatatctcag ccgccgagat
      901 ggatgccttt gcaaaacagg ctgcagtgat tatcgacgac gaagccgacg atgatgacga
      961 tttcggcgat gaagaatgag tatttccaga tagtggtaaa gtgccgtaaa gtagcgtcga
     1021 tgcacaggcg gctgaattgt ttatggtgaa aataaagttt tgatttttaa aaaggtgaat
     1081 tgaaaaatgc cgacgattaa ccaattgatt cgcaaaccgc gtaaagtaca gccggccaag
     1141 agcaagaatc ctgcgcttga ccagtgcccg cagcgtcgcg gcgtatgtct tctggtgaag
     1201 acggttaccc caaaaaagcc taactcggct cttcgtaagg tggctcgcgt gcgtcttagc
     1261 aatggcaagg aagttaacgc gtacattccg ggtattgacc acaacctgca ggaacacagc
     1321 atcgtgctgg ttcgcggcgg ccgtgttcgc gacctgccag gcgtacgtta tcacataatt
     1381 cgcggcacac ttgatacgct gggtgtcaat ggccggaaac aggctcgcag ccgttatggc
     1441 gccaaaaaac ctaaatgagt aaaaacgctg tttcgacagg taaagatttt ttggtttttg
     1501 cctgtgcggt tgttgagctg cgtttatcgg cagcttaaaa ccgtgaatgg ctgaaagctc
     1561 ataaatttgt taataaaaag taaatgactc acaatcggct gtgttggctg agagtactgg
     1621 agagtcgtaa atgtttcggt tttgtctgtt gtggctgatt atcgcggggc agacggctga
     1681 aacgaataaa aaaatcctaa cggaaaccag gaaaagttga gaggttaaaa atgggacgta
     1741 agaagtatac agcatcggca aagacgcttc agccggatcc gaaattcggc agcaaacttt
     1801 tggcaaagtt catcaatata ctgatggaag acggcaaaaa atatgccgct caggaaatta
     1861 tgtataaagc actggatatc gtcgccaaaa aagtcaaaga cgatactccg ttggttgttt
     1921 tcgaaaaggc tatcgataat ctcaagccgc gtatagaagt tcgctccaag cgagtcggcg
     1981 gtgcaaacta tcaggtgcca atgcccgtta attcacgccg tcagcagtcg ctggcgatgc
     2041 gctggctgcg ggatgcgatt cgtggcggca agggaagagc aacctatctt aaaacggcag
     2101 atgaaattat cagtgcgtac aaaggtgaag gtactgccat aactacgcgt gaaaacgttc
     2161 accgcatggc tgaagcgaac aaggcttttg cccacttcgc gtggtaatta aatctccggc
     2221 tgctgtgcaa aggtggtact ggcaataggc tgcctgagtt tattataaaa agataaaaat
     2281 aaacaaaaaa gcctcctgtc atttgacggg aggctttttt gttactttct cgcagaagat
     2341 cacgggtttg ccctggggta tctactttca tgctgagatt gcgaaccgct ctgtaaactc
     2401 actccagtag agcatgttaa atttgatatg cgtcatttct gctggctgca acttcgctct
     2461 gctgcgtcag gctgcatcgg aatacgggca gtattcctgc ttcgccttcc ttgcatagct
     2521 cgtttcgcca acgcataaag tgtcgcatct caagtttaat atgctctaat agcgatagtg
     2581 ttccggtttg tatggtcctt cgactggtac gccgatgtat ttcgcctgcg cgtcagtgag
     2641 tctggtgagc ttcgcaccga gcttgtccag gtgcagccgc gcaacttcct catccaactt
     2701 cttatccaga cgataaacgt caattttgcg gtccggattc ttgtgtatgt ctatctgtgc
     2761 cagcgtctgg tttgtgaagc tgttggacat tacgaaactc ggatggccgg tagcacagcc
     2821 gaggtttacc agtcggcctt ccgctagcag gtaaatgcag tggccgtcgg gaaatgtgaa
     2881 gcgatgtacc ggacaatgac tatccttgat ttcctctttt ttaatgccgg gaaaattctc
     2941 cagctcgtaa acctggattt cattatcaaa atggccgatg ttgcagacga tggcttgatc
     3001 tttcattctg ctcatgtgat tggcggtgag gatatccgta ttgccggtgg tggttacgta
     3061 aatgtcagcc agcggcaggg cgtcttcgac ggtgcatacc tgatagccgg ccatgcatgc
     3121 ctgcaatgca cagatggggt cgatttcact gaccatgacg cgacatttct ggccggcgag
     3181 cgattcggct gagcccttgc ccacgtcgcc gtagccgcag accatcgcca ctttgcccga
     3241 aagcattacg tccattgcgc gggatatgcc gtccaccagc gaatgccgac agccgtaaat
     3301 attgtcaaac ttgcttttcg tcacagagtc gttgacgttg attgcctgga aaagcagttc
     3361 gccgcgttct tccatctgca tgaggcgatg tacgcccgta gtggtttctt cgcttacgcc
     3421 gcggacattt ttggcgacct tgtgccagtg ttgcggatcg tgtttgagca tttttttcag
     3481 cagtgcattg attatctgaa gctctttgct ctttgtcggt tcgtcgagga tggcggcgtt
     3541 gttttccgca gcgtaaccgc ggtgtatcag cagtgtcgca tctccgccgt catcgacaat
     3601 ctggtcgggg ccgctgccgt ccggccatgt catagcctgt tgcgtacagt tccagtagtc
     3661 ttcgagcgtt tcgcccttcc acgcgaaaac gggtatgccg gctgccgcga tggcggctgc
     3721 cgcctggtcc tgcgttgaaa atatattgca cgaacaccag cgaacatccg cgccgagtgc
     3781 ggttaacgtc tctataagca cagccgtctg aatggtcata tgcagcgagc ccattatctt
     3841 tacgccggca agaggtttgt ctttgccgta tttttcgcgg gtggccatga ggccgggcat
     3901 ctcatattcg gcgatggtta tttcttttcg accggaatcg gacagtttga tatcagctac
     3961 cttgtaatcc ttggacatat agtgttctcc tgtacggtta aaattagaga gatattctac
     4021 caaaaagcga cgggagttcc aattaattga tgaggttttt gcgaggagag gcggaaatgt
     4081 tcatggaaat atcattgccc gacaaatccg tcatattcag cgtaactttt acaagaagtg
     4141 ttttggtggg ggcatcggcg ttggcgctta ctgcggcagt catgtttgtc acggttatgc
     4201 cgtcgtccgg ccccagcaat gatacggaac tgccgctttt
//
LOCUS       DAONFI010000199         4219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_25195_length_4219_cov_3.740154, whole genome shotgun sequence.
ACCESSION   DAONFI010000199 DAONFI010000000
VERSION     DAONFI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25195_length_4219_cov_3.740154"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PLK08_06995"
     CDS             <1..923
                     /locus_tag="PLK08_06995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPS53282.1"
                     /translation="TLPTPEEILTFIHKELSDKLISLNTFVTACCARFYLDKLRMDLV
                     DCGHTKTIHYRSLTNEYQFIKSSNLPLGFSQDEVYTQHSVHLKIGDVLLFYSDGLIET
                     KNSNDEDFGTERLVRFLADNSSLSPQQIVQKLYSELIHFSASVTFSDDLTYVVIKITD
                     PDAKQLLHKRNKSLLSNIENLEPLRIFIDQAVQELTGADITFISQFELAVSEATSNII
                     KHAYKRKSDQPIEVELNIYPDYLEINLFHWGEGLDDFQLITPAFDGTQENGYGLYLIR
                     NCVDEVNYSTDKVSGKARVRLAKYFRDAGA"
     gene            959..1300
                     /locus_tag="PLK08_07000"
                     /pseudo
     CDS             959..1300
                     /locus_tag="PLK08_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            complement(2178..3071)
                     /locus_tag="PLK08_07005"
     CDS             complement(2178..3071)
                     /locus_tag="PLK08_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HPS53283.1"
                     /translation="MQNKKPNHIISFDIEEYFQVESAAEAGVAGDDWAGYESRVEREV
                     DVILSILGEAGVCATFFVLGWVGMRHKILIRRIADYGHEIASHGMSHRMINRLKRDEF
                     YAELCDSKRILEDITGRTVKGFRAPTFSIMHSTAWAIDELCRAGYSYDSSIFPVHHDR
                     YGVPDAPRWIHRAMGPGGGEVIEIPPLTLRLLGGNLPAGGGGYFRLFPLWLTSHAIGA
                     AQRGGHAAMIYLHPWEFDPEQPVLPLGRLAKFRHRVGLGRTAGRLRTLLKRYTFGTAE
                     AHLSGCDDVSPDSFEYGAERD"
     gene            complement(3061..>4219)
                     /locus_tag="PLK08_07010"
     CDS             complement(3061..>4219)
                     /locus_tag="PLK08_07010"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53284.1"
                     /translation="EQATHQQTWAKRALAIDAVLSPAKVLERLNPQNADLYDSLIGMS
                     AAAAEGSDISPWTSRFSIYANDANSPAKLKSTVQRYRTLTEILKKSPATGVESQAAMP
                     KPVVDSLLNDKNRTKTAGMLILINVAADLDMPNLSLRLSRRLFEENRSCQWAVMDILS
                     FAPPLSELETLGGRITPADLFAAELVRAEIAFRRGDMENACGILGRLSEKYPDNTLLM
                     LRYGIALEQAGRLKDALKIYRKIWDTTGDVVAANNAASIVGQLFPKDPDELSAASELM
                     TKVLQVAPSIPAFLDTKGWIEYLRGNVPAARKYLLAGIMGLGDSIEAHYHVGMAEKDV
                     DAKMALWHFQAAVAYARKQLAAASPKLSAAEEHAMQESLGEIRRMEKNNAE"
BASE COUNT         1010 a         1183 c         1067 g          959 t
ORIGIN      
        1 ccacactgcc gacgcctgaa gaaattctca cgttcattca caaggaactc agcgacaagc
       61 tcatcagcct caacaccttc gtaaccgcgt gctgcgcgag attttatctc gacaagctgc
      121 gcatggatct tgtcgattgc ggccatacca agacaatcca ctatcgcagc cttacaaacg
      181 agtatcaatt cataaaaagt tcgaatctgc cgctgggctt ctcgcaggat gaagtgtaca
      241 cgcaacattc ggttcatctc aaaataggcg acgtactgct gttttactcg gacggcctta
      301 ttgaaacgaa aaattcaaat gacgaggatt tcggcactga gcggctggtg cgatttctgg
      361 cggacaactc cagcctttca ccgcagcaga tagttcagaa actttacagc gaactgatac
      421 atttttccgc ttcggtcaca ttcagcgacg accttaccta cgtggtaata aagattaccg
      481 acccggacgc caagcagcta ctgcacaagc gtaacaaatc actgttgagc aatatcgaga
      541 atcttgagcc gttgcggatt ttcatagatc aggccgtcca ggaactcaca ggcgcagaca
      601 taaccttcat cagccagttt gaactcgccg tgagcgaagc caccagcaat atcatcaaac
      661 atgcatacaa gagaaaatcc gatcagccga ttgaagtcga gctgaacatt tatcctgatt
      721 atctggagat aaatcttttt cactggggcg aggggctgga cgatttccag cttataacgc
      781 cggcattcga cggcacacag gagaacggtt acggccttta tctcatacgt aattgcgtcg
      841 acgaggtcaa ttacagcacg gacaaggttt ccggcaaggc gagggtacgg cttgccaaat
      901 atttcaggga cgcgggggca tgaaaaaaac attgacgtta atgtgggtta ttttcgcaat
      961 gctcataatc tacggcagtc ttatgccgtt cgacctgcac ttcgataaaa gcagcatcgt
     1021 tgagaatatc agacattcca cggaattctg gcccataggc cctgtcaggt ccggcaaagc
     1081 cgaccttgtg agtaacgtaa tattgtacgt ccccctggga ttccttgtcg cactcgccgc
     1141 cagagtcaac agaaaaagct caaccatgac agccatcgcc gctgccatgt ttaccggctg
     1201 cctgatgagt gcaggcatag aactgctgca agccatgtcg cagacccgcg tctgcggtgt
     1261 tcacgatttt ctgaccaacg ccgccggcac atttatcggc tctgtcgcag catgcgcata
     1321 tgggatgaac atttacagat tcgtcacggt aaagtgcagg caatactggc gtgactatcc
     1381 ggtggcattg gcggcgtgcg tgctggccgc agcattagca tgcgacgcac ttttcccatt
     1441 cctgccgact ttaaagctgc acgaaatccg ccagaactac cttaattcgc agttcagcct
     1501 cgccgccggt tttgacatgc acaccgccgg ctattggata gtgcagcaag ccggcgtatt
     1561 tgcgtttctg tcaatgcttg catgcagcgc gatacgcaaa gacggtcggc cgcagatatt
     1621 gaaggcactt gcattctgcg ttttatttgc agccatgctg gaaatcgcca aaatattcat
     1681 ctccaaccgg agcataaaca tcgccaacgt agtctccgcg gcagccggca gcgcgttcgg
     1741 agcagtaatg ttcgccgcac ttcacgacag gctgtcatat ggcgtaaagg tactgctgac
     1801 ggcagtcggc ctgctggtct acatggccta cctggaatgg ggcggagccg acctggaatt
     1861 tcacgccaat atggtcagac atctgcccaa aggcgtggaa tggctgccgc tgtacgatta
     1921 tgcgatgtcg ggccgaggtg aaaaggtagt actgttcgtc aggacgataa gcgtttccgc
     1981 ggcattcact ttcacggcac agcttctgaa tctcgacgaa aattacaggc ccaaaattct
     2041 gcgatgggcg attatcatgg ctttcgtcgg cggagtattc gaactcggcc agaccgtaat
     2101 gccccaaaga tatccgactc tgaccgacgt attctgcttc gccgtcggcg gagcggtcgg
     2161 cagcatattt acaatcatca atcccgttca gcgccatatt cgaaggaatc tggagagacg
     2221 tcgtcacagc cgctcagatg agcttctgct gtaccgaaag tataacgttt caaaagcgtc
     2281 ctcagccggc cagccgttcg gccaaggccg acgcgatgcc tgaatttcgc cagccggccg
     2341 agcggcagaa ccggctgttc agggtcgaac tcccatggat gcagataaat catggcagcg
     2401 tggccgccgc gctgagccgc accgattgca tgagacgtca gccacagcgg aaacagcctg
     2461 aaatatccgc cgccgccagc cggcagattg ccgcctaaca accgcaacgt cagcggcggt
     2521 atttcgataa cctcgccgcc gcctggcccc atcgcacgat gaatccagcg aggcgcatcc
     2581 ggcacgccgt agcggtcgtg atgaacggga aaaatcgatg aatcatatga atatccggcg
     2641 cggcagagtt cgtcaatcgc ccatgccgtg gaatgcatta ttgaaaacgt cggggctctg
     2701 aagcctttta ccgtccggcc ggtaatatcc tcaagaatgc gcttgctgtc acagagttcg
     2761 gcataaaatt catcccgctt cagacggttt atcatgcgat gcgacatgcc gtgcgatgct
     2821 atttcgtggc catagtcggc aatgcgacga atcagtatct tgtgacgcat acccacccag
     2881 ccgaggacga aaaacgtcgc acagacgcca gcttcgccca gtatggaaag aatcacatcc
     2941 acttcccgct cgacgcggga ttcatagccg gcccagtcgt cgccagctac gccagcctcg
     3001 gcagcggact ccacctgaaa atattcctct atatcgaaac tgattatgtg attcggtttc
     3061 ttattctgca ttgttttttt ccatccgtcg aatctctccg agcgattcct gcatggcgtg
     3121 ttcctcggcg gctgagagtt ttggagatgc ggctgcgagc tgttttctgg catacgccac
     3181 cgccgcctga aaatgccata acgccatttt cgcatccaca tccttttccg ccatgcctac
     3241 atggtaatga gcctcaatcg aatcgccgag acccattatg ccggctaaca gatacttcct
     3301 ggcagccggc acattcccgc gaagatattc tatccatccc ttggtatcca aaaacgccgg
     3361 aatcgacggc gcaacctgaa gaacttttgt catcagttca gacgcggcgg aaagctcatc
     3421 gggatctttt gggaaaagct ggccgactat cgacgcggca ttattcgccg ccactacgtc
     3481 accagtcgtg tcccatattt tgcgataaat tttcagggcg tctttcagac ggccggcctg
     3541 ctccagtgct atgccgtatc gcaacatcag taacgtattg tcaggatatt tttcagacag
     3601 acggcccaga attccgcacg cgttttccat gtcgccgcgg cggaaagcaa tttccgcgcg
     3661 taccagttca gccgcgaaca aatcggctgg cgttatccta ccgccgagag tttccagttc
     3721 ggacaacggc ggagcgaatg acagaatatc cataacagcc cactggcatg accggttttc
     3781 ttcaaaaagt ctccgcgaca gccgcagaga caggttcggc atatccaggt cagccgcaac
     3841 atttatgaga ataagcatgc cagccgtttt tgtgcggttt ttgtcgttca atagcgaatc
     3901 gactacaggc ttgggcatgg cagcctgcga ttcgacacca gttgccggcg attttttcaa
     3961 aatttccgtc agcgtacgat aacgctgcac cgtcgatttc agcttcgccg gcgagttggc
     4021 gtcgtttgca tatatggaga aacgggacgt ccacggcgat atatccgacc cttcggcagc
     4081 cgcggctgac atgccgatga gagaatcgta caggtcggca ttctgcgggt tgagccgttc
     4141 aagaacctta gccggcgaca ggacggcatc tatggcaagg gcacgctttg cccacgtctg
     4201 ctggtgcgtc gcctgttcc
//
LOCUS       DAONFI010000200         4189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_25467_length_4189_cov_3.547170, whole genome shotgun sequence.
ACCESSION   DAONFI010000200 DAONFI010000000
VERSION     DAONFI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25467_length_4189_cov_3.547170"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLK08_07015"
     CDS             complement(<1..638)
                     /locus_tag="PLK08_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPS53285.1"
                     /translation="MPRTLIQKILEEHLVEGELTPGSEIGVRIDQTLTQDATGTTSFL
                     LLEAMNIPRVRTEVSVSYVDHNMSQFGPENHNDHLYLQSISQKVGAWHSRPGNGICHQ
                     VHLERFAAPGRTLLGSDSHTPTGGGMGMIAMGAGGLDVAVAMGGGAFYMTCPQIIGVE
                     LRGRLQPWVAPKDIILRLLSILTTKGNVGCMVEYFGDGVINIDAPGRATCTN"
     gene            783..1529
                     /locus_tag="PLK08_07020"
     CDS             783..1529
                     /locus_tag="PLK08_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPS53286.1"
                     /translation="MAVLSMNFRGPRLGMTKTVNIITPKVEQAPGPWPVLYLLHGYSD
                     NHTEWLYNTSLVRYAWERNMMIVMPDGLRTWYTNINEEDAMEQFITSDLVKYIDGTFN
                     TIKSAAGRAIAGLSMGGYGAFMLSMKNPDMYCCASSHSGAFFYAHEIVKEMPQKVRTD
                     TERLAPEYDVFKLAADMPKDKRFPLRFDCGESDWILKWNRQMHEHLDSIGWSHTYNEY
                     PGEHNWAYWDSHICQTLDFAMENFAKSKLK"
     gene            complement(1559..2209)
                     /locus_tag="PLK08_07025"
     CDS             complement(1559..2209)
                     /locus_tag="PLK08_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HPS53287.1"
                     /translation="MIDIHTHTFLSDGLLCPAEHIRHAEMAGYRVLGIADHADLSTMA
                     DIIRILKVAAARENELGRLRVLAGVEFTHVRPEHLEEAAATARNLGADIVLCHGETIA
                     EPVIEGTNRAAILAGVDYLAHPGLITQADAELAAEKGVYLEISGKAGHSLANGHVAKM
                     ALKTGAKLMFGSDSHSPEQFFRREKAKRVLLAAGLDETQAEQVFANAEEMVKKLLR"
     gene            complement(2299..3795)
                     /gene="rpoN"
                     /locus_tag="PLK08_07030"
     CDS             complement(2299..3795)
                     /gene="rpoN"
                     /locus_tag="PLK08_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPS53288.1"
                     /translation="MNLSLTQNMRLSQEMKLSPRMIQAMEILQLPMLALQERIDAEME
                     SNPVLELCEDEDSPATEAGESETATDFDDRGEKLMVVSDERQNVDDFSRLDDMTAEFG
                     HDFANPDAPALSRRESLEASNRKLEAMANTPAPSESLDEYLCDQWRFVDASENVKKAG
                     FAVIEEIDSAGYLRTPAVEIADRLDMPDEDRLAVVNRAISLVQTLEPVGVGAIDLRQC
                     LLIQLDAMGQAGQDVTLPRQIVSQFLRELEMNRLPAIARKLNCTIDSVKAAIVVIGRL
                     NPRPGDEISTQSAQVIIPDLIVYIDENDKIVIKTAGGYIPDLTVSKYYIKEAKNKTND
                     RQTRSFLQKNIRSAHWLMDAIAQRQMTICRVAEEVFNFQREFLEKGVEALKPLPMSLV
                     AEKIGVHPATVSRAVAEKYVETPRGIFPLRMFFSGGTTSQDGHDMGWDAVKAKLREIV
                     DAEDKSRPLSDEKLADELEKAGLTVARRTIAKYREQLDIPPARQRKEF"
     gene            3919..>4189
                     /locus_tag="PLK08_07035"
     CDS             3919..>4189
                     /locus_tag="PLK08_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53289.1"
                     /translation="MMIRMGLFYEKKNIVGTAVVSTLCMLVGAAGGSRIAIIYANNRL
                     MSGWWATTSYLLSLPITRIGMIVGGCCGIIGGYFWCRYMAHRTKFY"
BASE COUNT         1013 a         1142 c         1031 g         1003 t
ORIGIN      
        1 atgttcgtac acgtggcccg gccaggtgcg tcgatattga taacgccgtc gccgaaatat
       61 tcaaccatgc agccgacgtt acctttggtg gtcaatatgc tcaacagacg caggatgata
      121 tctttcggcg caacccacgg ctgaagtctg ccgcgaagct ccacgcctat aatctgcgga
      181 cacgtcatat aaaacgcacc gccgcccatg gctaccgcaa catcaaggcc gcctgcgccc
      241 atggctatca tgcccatgcc gccgccggtc ggcgtgtggc tgtcgctgcc gagcagcgtc
      301 ctgcctggcg cggcaaaacg ctccaaatgc acctgatgac atatgccgtt gccaggccgg
      361 ctgtgccaag ccccaacttt ctgcgatatc gactgcaaat acaggtggtc gttatgattt
      421 tccggcccga actgggacat gttgtgatcc acgtaactca ccgatacctc ggttcgaaca
      481 cgaggaatat tcatcgcttc cagcagcagg aacgacgtcg tgccggtggc gtcctgcgtg
      541 agggtctggt ctattcgaac cccgatttca ctgccgggcg taagttcacc ttcgacaagg
      601 tgttcttcaa gtattttctg tataagcgtt cttggcattt cagactccat atgtttaaag
      661 cattttcgca atctttcaaa gattagggat tttagcagat tcagcccgaa aaaccaaatc
      721 taaaaacgtc ttgcaagttg caggcccagc cagtaaaaca gaccggcatt caggagaaaa
      781 cgatggctgt tctgagcatg aatttccgcg ggcccaggct gggcatgaca aaaacggtta
      841 acatcatcac cccgaaagtg gagcaggctc cagggccatg gccggtgctc tatctgttgc
      901 acggatatag cgataatcat accgaatggc tgtacaacac ttcgctggta cgctatgcgt
      961 gggaacgaaa tatgatgata gtaatgcccg acggcttgcg gacgtggtac acgaacatca
     1021 acgaagaaga cgccatggaa cagttcatca catccgacct cgtaaaatat atcgacggca
     1081 cgttcaacac gataaaatcc gccgccggcc gagccatcgc cggcctgagc atgggcggct
     1141 acggcgcgtt catgctgtcg atgaaaaatc ccgacatgta ctgctgcgcg tcgagccatt
     1201 caggcgcatt tttctacgca cacgaaatcg taaaggaaat gccgcaaaaa gtcagaaccg
     1261 acacggaacg gctcgctccg gaatatgacg ttttcaaact ggcggctgac atgccgaaag
     1321 acaaacgttt tccgttgcgg ttcgactgcg gcgaatcgga ctggattctg aaatggaatc
     1381 ggcagatgca cgaacatctg gattcaatcg gatggtcgca cacgtacaat gaataccccg
     1441 gcgaacacaa ctgggcgtac tgggactcgc atatctgcca gacgcttgat ttcgcaatgg
     1501 aaaatttcgc aaaatcaaaa ttaaagtgaa cagactggtg cgccgctcgt cgccaaattc
     1561 agcgcaggag tttttttacc atttcttcgg cattggcgaa aacctgttca gcctgtgttt
     1621 cgtccagccc ggcagctaac agaacacgct ttgccttctc ccgcctgaaa aactgctccg
     1681 gcgaatgtga atccgagcca aacatcagtt tcgcgcctgt ttttaaagcc attttcgcaa
     1741 catggccgtt agccagcgaa tggcccgctt tgccggaaat ttccagataa acgcccttct
     1801 ccgccgccag ttccgcatcc gcctgagtaa tcaggcccgg atgagccaga taatccacgc
     1861 cggcaagtat cgcagccctg ttagtgccct cgataacagg ttctgcaatc gtctcgccgt
     1921 gacagagaac gatatcagcc ccaagattgc gagccgtagc cgcagcctct tccagatgtt
     1981 ccggccgcac atgcgtaaac tccacgcctg ccagaaccct caggcggccc agctcattct
     2041 cgcgtgccgc cgccactttc agaatgcgga tgatgtcggc catggtagac aggtcggcat
     2101 ggtcggcaat gccgagcaca cgataaccgg ccatttccgc atggcgaata tgctccgccg
     2161 gacaaagaag cccgtcggaa agaaacgtat gagtatgtat gtctatcatg ttattccttt
     2221 tcaaatctca aaaagacgtt tatccaatat tttgtttaaa gatagttatt tcatatcgag
     2281 atgctctgaa aaacatttct aaaattcttt tcgctgtctt gcgggcggta tgtccagttg
     2341 ctcgcgatat ttggctatcg tacgccgggc tacggtcaag ccggcctttt caagctcatc
     2401 ggcaagtttt tcatcgctca gcggacgcga cttgtcctcc gcatccacta tttcccgcag
     2461 tttagccttg actgcgtccc agcccatatc atggccgtcc tggctggtcg tgccgccgga
     2521 gaaaaacatg cgaagcggaa aaatcccgcg cggcgtttca acatattttt cggcaaccgc
     2581 cctgctgacg gtggctggat gtacgccgat tttttcagct accagcgaca tgggcaacgg
     2641 cttgagggct tctacgcctt tttcaaggaa ttcgcgctga aaattaaaca cctcctcggc
     2701 tacgcgacag atggtcatct gccgctgtgc gatggcgtcc atcagccagt gagccgaacg
     2761 gatatttttc tgaagaaaag agcgagtctg gcggtcgttg gttttatttt tcgcttcttt
     2821 tatatagtac ttgcttacag tcaggtccgg aatgtaaccg ccggcggttt ttatgacaat
     2881 tttgtcattc tcgtcaatat aaacaatcag gtctggaata ataacctgcg cggattgcgt
     2941 tgaaatctca tcgccgggcc ggggattcag ccgcccaatg acgacaatgg ccgcttttac
     3001 gctgtcgata gtacagttga gctttctggc gatggccggc agacggttca tctccaattc
     3061 acgcaaaaac tgcgatacaa tctgtcgcgg caatgtcaca tcctgccccg cctgccccat
     3121 agcatcgagc tgtatcagca gacactgtcg caaatcaatc gcaccaacgc cgaccggctc
     3181 aagcgtctgc acgagcgata tggcgcggtt gacgacggca agcctgtctt cgtcgggcat
     3241 atcgagtctg tcggcaattt cgacagccgg cgtacgcaga taaccggcag aatcgatttc
     3301 ttcaattaca gcaaaaccgg cctttttcac attctcagac gcatccacaa aacgccactg
     3361 atcacagaga tactcatcga gcgattccga cggcgcaggc gtattagcca tcgcttcaag
     3421 tttgcgattg gaggcttcga gactttcgcg gcggctgagc gcgggagcat cgggattcgc
     3481 aaaatcatgg ccgaattcag ccgtcatatc gtcaagccgg ctgaaatcgt caacattctg
     3541 ccgttcgtcc gaaactacca tcagcttttc gccgcggtca tcaaaatccg tggcggtctc
     3601 ggactcgcca gcctcagtcg caggcgaatc ttcatcttca caaagctcca gtacaggatt
     3661 ggattccatc tcggcgtcta tccgctcctg aagtgccagc atgggtaatt ggagaatctc
     3721 catcgcctgt atcatacggg gcgagagttt catctcctgc gaaagccgca tattctgtgt
     3781 aagactgagg ttcatgcaat ctccatgcca aattttccta aaatcagttt ataacacttc
     3841 gtaaaatatt gtcaacatca ttcataaact tgattttttt tggcaaaaga attgttgaat
     3901 acgcatttac aatgagttat gatgataaga atgggcctgt tctatgaaaa aaagaacatc
     3961 gtcggcacag ctgtcgtaag tacgttatgc atgttggttg gtgcggctgg cggctcacga
     4021 attgccatca tttacgccaa caatcggctg atgtccggct ggtgggcgac gacgtcgtat
     4081 ctcctgagcc ttccgataac caggattggc atgattgtgg gaggctgttg cggcattatc
     4141 ggcggatatt tctggtgcag atatatggcc catcgaacga aattctatc
//
LOCUS       DAONFI010000201         4179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_25548_length_4179_cov_4.411251, whole genome shotgun sequence.
ACCESSION   DAONFI010000201 DAONFI010000000
VERSION     DAONFI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25548_length_4179_cov_4.411251"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..817)
                     /locus_tag="PLK08_07040"
     CDS             complement(32..817)
                     /locus_tag="PLK08_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPS53290.1"
                     /translation="MSGIKQSFVYELFRQDDVPTEELLKFAARTGFAAVEAWYLGQDR
                     EEFIALAHSAGLRVASFIGHHEVKIGMNNPDNHAAIESELRENIEFARRYDIPGLICL
                     SGDLPENADVQVATDSIVKILSRVKLAAEKAHVNLNLEVLNSKLDHEGYQIDSTAVAV
                     EIIKRVESPNVKILYDIYHMQIMEGDLIRTIKENIGYIGHFHTAGVPGRHDLDDSQEI
                     NYPAVMKAIAATGYNLYVGHEFLPLSTDRMEALAKAFRICNVK"
     gene            complement(869..2203)
                     /locus_tag="PLK08_07045"
     CDS             complement(869..2203)
                     /locus_tag="PLK08_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPS53291.1"
                     /translation="MANEKNKLAINGGEKAVPGYEGINHPKIGIEEFMELVDTWGYSV
                     NVKNEIRRLVETERDIPEPHLARYYHPKQSKVALLEEYAKNFFGTKHALAVNSGTSAL
                     IAAYIGAGIGPGDEVIVPGYTFFATVSAVVVARAIPVIAEIDETMTLDPEDVERKITP
                     QTKAIVPVHIAGHACNMDAIMEIARKHNIKVIEDTAQACGGMYKGRRLGSIGDAGCFS
                     LSTWKITGAGEGGMVITNDDMTYIKAQGQHDTAACWRPDRFGKERMPGELFCGENYRM
                     SELEGSVIYVQMQKSGAQFERYNTNMRRIASQIGKYDRIKLRPSNDINGDVGYILTLL
                     PQTPGAATELATALAAEGVPAGARGTKVSRDWHVYAFWEQILQQKTATPEGCPFTCPL
                     YKGMLPKYSQDMCPRTMDIFDRAVNIAVNQWWTPRDCDNIAAAINKVCRVLG"
     gene            2347..3198
                     /locus_tag="PLK08_07050"
     CDS             2347..3198
                     /locus_tag="PLK08_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53292.1"
                     /translation="MTISNQQCDRIAAIPKICFLNAAEFTMTYTTPSNARCVKTWLIE
                     LVTKGSFSLNVENSGWQSRSKGAGIIYAPQTLGCEDADRNLKVNRPTTSLYIEFTLEN
                     DDFLYEILKANGKFAWIDDSADVLQSLMADLIRSTNSGIPYSELYTVGTFYQILSLLI
                     NSDKRGDIIVTGQPLSLMPDMVLKANLYMQKNMASPIRICDIASYVGMSESGFSHAYV
                     RITGKSPLTVLRAFRVQAARNMLVKGNFTLQMIAELTGFSDAFHLSRYFKQVMKMSPR
                     EYLKSSR"
     gene            complement(3203..4006)
                     /locus_tag="PLK08_07055"
     CDS             complement(3203..4006)
                     /locus_tag="PLK08_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPS53293.1"
                     /translation="MKNVKKQFSRIEYSPDDSYCLEYSSDTDSLRDWAIISPGRLRTW
                     IVCLHGHGSHGDQLFTCEDIRKAWLCEFISTGAGIICPNLRDNAWMSPAAVSDLHDIL
                     DFLRREHGAGKFVFYAGSMGGTGNLIYATRHPEDVAACVALGAATDLASFYEWCMNQP
                     EEFIANQLGRAIEESYGGKPTVRPQLFAEHSTLKNCENLNMPIFFAHGEKDQLIPIGQ
                     ARMLAARMATYKNFVYHEVAGGDHGSPLFLPEAMEFLKYHISNPNRLGG"
BASE COUNT         1042 a         1077 c          890 g         1170 t
ORIGIN      
        1 aacattcgct cctggcggct ctgccgctgt ttcacttcac attacatata cggaatgcct
       61 ttgccaacgc ttccattcta tcggttgata acggcaagaa ttcatgccca acatataaat
      121 tatatcctgt tgcagcaatt gctttcatca ccgccggata attaatttcc tgcgaatcgt
      181 caagatcatg ccttccaggc acgccggcag tatggaaatg gcctatatat ccgatattct
      241 ccttaatcgt ccgaatcaga tcgccctcca taatctgcat atggtagata tcgtagagaa
      301 tcttcacgtt cggcgattcc acgcgtttta ttatctccac cgcgacggcg gtgctgtcaa
      361 tctgatatcc ctcgtgatcc aatttgctgt tcagcacttc aagattaaga ttgacatgtg
      421 ccttttcagc agcaagcttc accctggaca gaattttaac aatcgaatcc gtcgcaacct
      481 gcacatctgc gttttcaggc agatcgccag acaggcatat gagtcctggt atatcgtacc
      541 gccttgcgaa ttcaatgttt tctcgcagct cagactctat ggcggcgtga ttgtccggat
      601 tgttcatgcc tatcttgact tcatgatggc cgatgaaact ggctacccgc aaacctgccg
      661 aatgcgccag agctataaac tcttccctgt cctggccgag ataccatgct tccacagcgg
      721 caaatccggt acgggccgca aattttaaaa gctcctccgt cggcacgtcg tcctgcctga
      781 acagttcata aacgaatgac tgctttattc ctgacatttt aaaaacctct tttaatattt
      841 taaatatatt atgttgcggt tcgtccgatt atccaagtac gcgacagacc ttgttgatgg
      901 ctgcagctat gttgtcgcag tcccttggcg tccaccactg atttacggcg atatttacag
      961 ctctgtcaaa aatatccatc gtacgcgggc acatatcctg tgaatatttc gggagcatac
     1021 ctttgtacag cggacaggta aacggacagc cttcaggcgt ggcagtcttc tgctggagaa
     1081 tctgttccca gaatgcgtat acgtgccaat ctctcgatac cttcgttcct ctcgctccgg
     1141 ctggtactcc ctcggcagcc agcgccgtgg caagttccgt ggcggcgccg ggcgtctgcg
     1201 gcaaaagcgt tagaatataa ccgacatcgc cgttgatgtc gttgctggga cgaagcttta
     1261 tcctgtcata ttttcctatt tgcgaggcta tccgcctcat attcgtattg taacgttcaa
     1321 actgtgcgcc tgatttctgc atctggacgt atatgaccga accttccaac tcggacatac
     1381 ggtagttttc gccgcagaaa agttcaccag gcattcgttc cttgccgaat cggtcggggc
     1441 gccagcaggc tgccgtatca tgctggccct gtgccttgat atatgtcatg tcgtcattcg
     1501 tgatgaccat gccgccttcg cctgcgccgg ttattttcca agtactcagg ctgaaacatc
     1561 cggcatcgcc tatcgagccc agccggcggc ccttgtacat gccaccgcag gcttgcgccg
     1621 tatcctctat caccttgata ttgtgtttgc gggctatttc cattatggca tccatattgc
     1681 aggcatgtcc tgctatgtgc acaggtacaa tagccttcgt ctgcggagtg attttgcgtt
     1741 caacatcctc aggatcaagc gtcatggtct catctatttc cgcaatgacc ggaattgccc
     1801 tggccaccac cactgccgat acagtcgcaa aaaaagtgta tccaggcacg ataacttcgt
     1861 caccaggccc gattccggca ccaatgtatg ctgcaatcag cgctgaagtt ccggaattca
     1921 cggcaagggc gtgtttcgtt ccgaaaaaat tcttggcata ttcttccagt agtgcaacct
     1981 tggattgctt cggatgataa tatcgtgcca gatgcggctc agggatgtcc cgctccgttt
     2041 cgaccagcct gcgaatttca ttcttgacat tgacactgta accccatgtg tcgacgagtt
     2101 ccataaattc ttcaatacct attttgggat gattgattcc ttcatatccg ggaactgctt
     2161 tttctccgcc gttgatggcc agcttgtttt tttcatttgc catgataaga ctcctgaaac
     2221 attgattaaa ttgctttaca catgattttt acatttgcca ttctgaacaa tatactctat
     2281 attatatgga ggatatttca ttattacaat gtatgcgact gtcgaaaaca tgtaaaatac
     2341 tgcacaatga ctatttcaaa tcaacaatgt gaccggattg ctgcgattcc gaaaatatgt
     2401 tttctgaatg ccgccgaatt caccatgaca tataccacgc cgtccaatgc gcgatgcgtc
     2461 aaaacgtggc tcattgaact cgtaaccaaa ggaagttttt ccctgaacgt cgaaaacagc
     2521 ggttggcaat cccgctcaaa aggtgccggc ataatttatg ctcctcaaac tctgggctgt
     2581 gaagatgccg accgaaacct taaagttaat cggccaacga ccagccttta tatcgaattc
     2641 acacttgaaa atgatgattt tctgtacgaa attttaaaag ctaacgggaa attcgcatgg
     2701 attgatgatt ctgccgacgt acttcaatcg ttaatggccg acttgatacg aagtacgaac
     2761 agcgggattc cgtattcaga actatacaca gttggcacat tttaccagat tctgtcgctg
     2821 ttaatcaatt cagataaacg cggcgatata attgtgacag gccagccgct ctcgcttatg
     2881 cccgacatgg tactcaaagc caatctttac atgcaaaaga acatggcgtc acccatccgc
     2941 atttgcgaca tcgccagtta tgtcggaatg agcgagtccg gtttttctca tgcttatgtg
     3001 agaattaccg gaaaaagtcc gctgacggtt ttgagggcat tcagagtgca ggctgcaagg
     3061 aatatgctcg tcaagggcaa ttttactttg cagatgatag cggaattgac aggtttttcg
     3121 gacgcatttc acctgtcaag atattttaaa caggtcatga agatgtcgcc tcgcgagtat
     3181 cttaaatcat ccaggtaatt tgtcagccgc caagcctgtt tggatttgaa atatggtatt
     3241 ttaaaaattc catagcctca ggcaagaaca gcggcgaacc gtgatcgccg cctgcgactt
     3301 catgatacac gaaatttttg taagttgcca ttcttgcagc cagcattctt gcctgcccaa
     3361 tcggaataag ctggtccttt tctccgtggg caaagaatat cggcatattc agattttcgc
     3421 aattttttaa ggtggaatgt tctgcaaata actgtggcct gaccgtcggc ttgccgccgt
     3481 atgattcttc gattgcccgg ccaagctgat tggcaataaa ttcttcgggc tgattcatgc
     3541 accattcgta aaatgaggcc aaatcggtgg cagcgccaag tgcaacacac gcggcgacat
     3601 cctcaggatg ccgcgtcgca tatatcagat tccccgtccc gcccatggac ccggcataaa
     3661 acacgaattt tcccgcccca tgctcccgtc gcagaaaatc aagaatgtca tgcaaatcac
     3721 tcactgccgc cggcgacatc catgcgttat ctcgcaaatt cgggcagatt atgcctgcac
     3781 ctgtcgaaat gaattcacac agccacgctt tgcgtatatc ttcacaagta aataactggt
     3841 cgccatggct tccatggccg tgaagacata caatccacgt tcgtaaccgg ccaggagaga
     3901 ttatggccca atcgcgtaac gaatccgtat cacttgagta ttcaagacaa tacgagtcat
     3961 ccggcgaata ctcaatccta gagaactgtt ttttgacgtt tttcatcgcc caaccatttt
     4021 tactgatttt actgaatatt cccaaatatt ttcatttgat gcttttacat aatggaagac
     4081 gttcgccttt tgcatcctga ttcatgaata aatacttgca gggatacagt atagcactta
     4141 atgtcgccat cagcaatgaa cacaactgct caatacatg
//
LOCUS       DAONFI010000202         4175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_25586_length_4175_cov_4.207039, whole genome shotgun sequence.
ACCESSION   DAONFI010000202 DAONFI010000000
VERSION     DAONFI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25586_length_4175_cov_4.207039"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLK08_07060"
     CDS             <1..1097
                     /locus_tag="PLK08_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53294.1"
                     /translation="DLDTMQIQDTGLRFPPKGRKGTYAYSVGDKIHSAMILGNGKFAK
                     TLLLGHGSHIWWDDGGWPFDARIFQGGALYSYKPASKKLECLGIPVQRNTIHGLFGGD
                     GFAAGYSLPDNHFFSFDYETGEMCDFGKISAYCCHSLGCVGKKVFGVYRRSMGEQRGD
                     AVVANNSGAFLFFYDHDARQLERTEILIDPLETNIRFNSGMDSWVSTPAGLIGGRVDG
                     TLFLLDTERLAIEELCYAIEPTHPSLSREQIRKLGGSRCTAINGSERITSMEMLDKNH
                     IIGTAGFPIMHVFVYDLAARRSFDLGPVNPDTDMCYFHDMVITRPDAKNIRLALAETD
                     SGLANLHILNMPAEKICQLTSQEELLANPI"
     gene            1151..3118
                     /locus_tag="PLK08_07065"
     CDS             1151..3118
                     /locus_tag="PLK08_07065"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPS53295.1"
                     /translation="MKAEIFEIPQCFNMGFHYTIQFSRRTRVCVMGLVDQSSEEWERW
                     YLRMHEKEALREIADAGYTIIEIHFLYGFGLKSEKEEWERTRIMVQNAHEVGLRVFGY
                     FQFFSVQEETFFLENPWAKKCVQIDAKGNRIQYRYDRPALCFSNAKVRQYYLDGIELG
                     LKTCDLDGIRLDNDYYRGCYCERCQAAFRTWLTETFDKASAKRIFGLSSLKGMSLVPV
                     LDNHDPLWLATVKFRQKQRQDIMLTLSEKIHEIKPQAIFGGNPAISRRFNDTYRINVY
                     PPDLGKTHHLVCAENLRFPARTGESTRHQAVLYKYGQSSNYSIFVSHHLYRNDGSMRW
                     PDSVEECLLSFCEGLAFGGHPVTTTWGLRMDGNENHTLYQRPIFLQALDIVSKFIKNN
                     SDIYKNATCNAVIGIYQNRENLCMDSLNTWLSLQGAVQAFLQHQIPFRFVDHDDRHLY
                     DGLQVLVIPDVKYVSKNDMEQFLDFMNNGGKLLVTGNSCTHDEWGLPHEGEYPDKLWK
                     HPNVRKLVGTPEKIAFASEHEFNKMVGDETGGMWYKDLPLPEGHEKFVKNVRELLSVE
                     KVHVESSPFIAIDTFANDDGEQFLHLLNYDNRNPSDVSVIIDGNIKDLQCLMPDGIGI
                     QNVPDIHFLGDKTKLNLVGLKTYCVIRYRFV"
     gene            3247..4002
                     /locus_tag="PLK08_07070"
     CDS             3247..4002
                     /locus_tag="PLK08_07070"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HPS53296.1"
                     /translation="MGLISQYHHSIEKILQEIEENEEKHIQQAATLLAEKVVQGRLIN
                     IFGAGGHSAIAGMEVFYRAGGLVPVNPIFPPGMNVLESRPTMSRLAGTGEFILNYYQV
                     EADDILIVVNFYGLNPASIDTALAAKKRGTKLITINSHLFASQIPKNFRWRHPTKLNL
                     NELADVAIDNHVPKPDAVLRVPGIENPLSASATIATCFTLNALMAETAVAIKRLGKEP
                     EIWLSNNIPGGDEHNDEYIKKYRSRIHFLYPVS"
BASE COUNT         1220 a          997 c          958 g         1000 t
ORIGIN      
        1 aggaccttga cacaatgcag attcaggaca caggcctgcg attcccgcca aaaggcagga
       61 aaggcactta tgcctattca gtaggcgaca aaatccacag tgccatgatt ctcggtaacg
      121 ggaaattcgc aaaaactctc ttattggggc atgggtcgca catatggtgg gatgacggcg
      181 gctggccatt cgacgccaga atttttcagg gcggagcttt atattcatat aaaccggcgt
      241 caaaaaagct ggaatgtctc ggcattccgg ttcaaagaaa cacaatacac ggcttatttg
      301 gcggagatgg ttttgcggca ggctattcat tgcctgataa ccactttttc tccttcgatt
      361 atgaaacggg cgaaatgtgc gactttggta aaataagcgc gtactgctgc catagcctgg
      421 gatgcgtcgg gaaaaaagtg ttcggcgttt atcgtcgttc aatgggagaa cagcgcggcg
      481 atgccgtagt ggcgaacaat tccggcgcgt tcctgttctt ctacgaccac gacgccagac
      541 agcttgagcg aacggaaatt ctcattgacc cgcttgagac caatattcgt ttcaacagcg
      601 gcatggattc atgggtcagc actcctgccg gcttaatcgg cggacgagtc gacggcactc
      661 ttttcctgct cgataccgaa cgacttgcaa tcgaagaact atgctatgca atagagccga
      721 cgcatccaag tctttccagg gagcaaatca ggaaacttgg cggcagtcgc tgtacagcca
      781 taaacggctc ggaacgcata acgtccatgg aaatgctgga taaaaaccat attatcggca
      841 cagcagggtt tcctataatg catgttttcg tttacgacct tgccgccagg cggtcattcg
      901 atctcggccc cgtcaatcct gatactgaca tgtgctattt tcatgacatg gtaattaccc
      961 ggccggatgc aaagaacatc cgacttgcac ttgctgaaac cgattccgga ttggcgaact
     1021 tacatatatt gaatatgccg gcggaaaaaa tctgtcaact gacatcgcag gaagagttgc
     1081 tggcaaatcc gatttgacgc aaactcattg cgacgaaatt taacaatgca ttcatgaggc
     1141 gaacacgcat atgaaagcag aaatattcga aattccacaa tgcttcaaca tgggttttca
     1201 ttacaccata caattcagcc gccgtaccag ggtctgcgtg atgggcctgg tcgatcaaag
     1261 cagcgaagag tgggaacgat ggtatttacg catgcacgaa aaggaagcat tgcgtgagat
     1321 agcagacgcc ggttatacaa taattgaaat acatttcctg tatggttttg gacttaaaag
     1381 tgaaaaggaa gaatgggaac gaacccgtat aatggtacaa aacgcacatg aagtcggctt
     1441 gcgcgtgttc ggttattttc aattcttttc agtccaggaa gaaacttttt tcctcgaaaa
     1501 cccctgggcg aaaaaatgcg tccagattga cgccaagggc aatcgaattc aataccggta
     1561 tgaccggccg gcgctatgtt tcagcaacgc aaaagtacgg cagtattatc tcgacggcat
     1621 agagctgggc ttgaaaacct gcgacctcga cggaataaga ctggataacg attattacag
     1681 gggttgctat tgcgagcgat gtcaggctgc tttccggaca tggttgacag aaacattcga
     1741 caaagccagc gccaaacgca ttttcggtct atcatctctc aaaggcatgt cgcttgttcc
     1801 cgtactagac aatcacgatc cgctatggct ggcaacggtc aaattccgcc agaaacagcg
     1861 gcaggacata atgcttaccc tgtctgaaaa aattcacgaa ataaagccgc aagccatttt
     1921 cggcggcaat ccagccatca gccgccgttt taacgacacg tacagaatca acgtttatcc
     1981 gcctgatttg ggcaaaacgc atcatcttgt ctgtgcagaa aatctccgct ttccagcccg
     2041 cacaggcgaa agcacccgtc accaggccgt cttgtacaaa tacggccaat catccaatta
     2101 ttcgatattt gtcagccatc atctttaccg gaacgacggc agcatgcgtt ggccggattc
     2161 agtggaagaa tgtctgctat cattctgcga aggccttgcc ttcggcggcc accccgttac
     2221 cacaacatgg gggctcagaa tggacggcaa cgaaaatcac acgctttatc aacggcccat
     2281 attccttcag gcgctggata tcgtctcgaa attcatcaaa aataattcgg atatctataa
     2341 aaatgcgaca tgcaacgcag taataggaat ttatcagaat cgagaaaacc tttgcatgga
     2401 ttcgctcaac acgtggttat cgttacaggg cgcggtgcag gctttcctgc agcatcagat
     2461 tccattccga ttcgtagacc acgatgacag acatctttac gacgggctgc aggtgcttgt
     2521 gattccggac gtaaaatacg taagcaaaaa cgacatggaa caattcctcg atttcatgaa
     2581 caacggcggc aaattgctcg taaccggcaa tagctgtact cacgacgaat ggggtctgcc
     2641 gcacgaaggc gaatatcccg acaagttatg gaaacatcca aacgtcagaa aactggtcgg
     2701 aactccggaa aaaatcgctt tcgcatcaga acatgagttc aacaaaatgg tcggagacga
     2761 aacaggcggg atgtggtata aggatcttcc gctccctgaa ggccatgaga aattcgtcaa
     2821 aaacgttcgc gaacttttat ccgtcgaaaa agtccacgtc gaaagttctc cgtttatcgc
     2881 gatagataca ttcgccaacg acgacggcga acaattcctg catctgctta attacgacaa
     2941 cagaaatcca agtgacgttt cggtcattat cgatggaaac attaaagatt tacaatgtct
     3001 catgccggat ggcataggaa tacagaacgt tcctgacatt catttcctcg gcgacaaaac
     3061 gaaattgaat cttgtaggtc tgaaaacata ctgcgttatc cgttacagat ttgtttaaaa
     3121 gtttttatca aaatgaaaca tggcaccaag gcacggttgg ccgaatatcg tagcattttt
     3181 taaaaatttt catcttgaaa atcgctccag acgtttttta gagtgttttt tacaaaggga
     3241 atggttatgg gtcttatttc acaatatcat cacagcatcg aaaagatttt acaggaaatc
     3301 gaagaaaacg aagaaaagca tattcaacag gcagcaaccc tgcttgctga aaaagtcgtg
     3361 cagggccgct taataaatat ttttggcgca ggcggccatt cagccatcgc cggtatggaa
     3421 gttttttaca gagccggcgg ccttgtgccg gtcaacccga tatttccgcc tggcatgaac
     3481 gtacttgagt caaggcctac gatgtcaaga ttggctggca ctggtgaatt cattctgaat
     3541 tattatcagg ttgaggccga cgatatactt atcgtagtga atttttacgg cctcaacccc
     3601 gcatcaattg acacggcact ggcggcaaaa aaacgcggca caaaacttat tacaatcaat
     3661 tcacatctgt ttgccagcca gatacccaaa aattttcgat ggagacatcc aaccaaatta
     3721 aatcttaacg aacttgccga tgtcgccatc gacaatcatg tgcccaaacc cgacgcagtc
     3781 ctgagagtac ccggcataga gaatcctctc agcgcatcag ccaccattgc cacgtgtttt
     3841 acactcaatg ccctcatggc tgaaacggcc gtagccatca aacgtctcgg aaaagaaccg
     3901 gagatatggc tgagcaacaa tattccaggc ggcgatgaac ataatgatga atacatcaag
     3961 aaatacagaa gcagaattca tttcctgtat cctgtaagct agagcatcac gtagaacatg
     4021 gcgtttcaac gtttcaaacc atcagtatcc caattacagc cggctattta tgtagaacct
     4081 atcccaaaaa catgggcgtc gccgaaatcg agcaatttta cgggctggca aggaagaaaa
     4141 atggcagcat atttaaggat attctgccgt ttttc
//
LOCUS       DAONFI010000203         4140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_25894_length_4140_cov_4.531946, whole genome shotgun sequence.
ACCESSION   DAONFI010000203 DAONFI010000000
VERSION     DAONFI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25894_length_4140_cov_4.531946"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..943)
                     /locus_tag="PLK08_07075"
                     /pseudo
     CDS             complement(1..943)
                     /locus_tag="PLK08_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735402.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
     gene            1233..2249
                     /gene="gap"
                     /locus_tag="PLK08_07080"
     CDS             1233..2249
                     /gene="gap"
                     /locus_tag="PLK08_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPS53297.1"
                     /translation="MAVKIGINGFGRIGRLVFRVMAEQPERFDIVGINDLGKAELLAY
                     LAKYDSTQGRFPGKIVAKQNALVVNGKEIPVFSEKDPANLPWGKLGVDVVIESTGIFT
                     NAKADGKAGYDSHLDAGAKKVIISAPAKGFASTVVMGVNDDIITPEVTCVSNASCTTN
                     SLAPVVKVLHEKFGVVKGLMVTCHGYTNDQHVLDQIHKDPHRARAAAINIIPTSTGAA
                     VAVGKVLPELNGKLDGYSLRVPVPTGSITDLTVEFAKDVDVESINEAVKAAAEGEMKG
                     IIEYNTDPIVSSDIIHNSHSSIFDAGNTRVIGGNMAKVTMWYDNEWGYSNRTADLAEK
                     LAIL"
     gene            2402..3208
                     /locus_tag="PLK08_07085"
     CDS             2402..3208
                     /locus_tag="PLK08_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPS53298.1"
                     /translation="MKDKMNIWQGKHKIPWDDYDFSRRMLAEHLTQEHDMASRRIEWI
                     ERQVTWIHKNLFDGQPSKIMDLGCGPGLYLHRLVKLGHICCGVDFSPASIEYANLHNP
                     DRVNCIFFKSDIREITFGVDYDLIMMLFGEFNTFSPAEALSILRKSCDSLHNHGGRMI
                     LEISTPQAVSRIGRSESSREEAESGLFSDEPYVCETSNRWLPEYNAAVQTFRVEEKST
                     EKTTIYRSTTRALSDDELRELIMSAGFASATRLREWPCNTDDLQLWLIEK"
     gene            complement(3346..>4140)
                     /locus_tag="PLK08_07090"
     CDS             complement(3346..>4140)
                     /locus_tag="PLK08_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS53299.1"
                     /translation="TEQRLHIKDMAAIYVNTAQKFEHDAIFIHENPGEVEILMQIVDE
                     IKAMSGDDYFLMCHGDATFAIPDGGKMEEWSFRMIDEPEKVDAKLAQRVDDAIVRAEK
                     IKTHGGMDAFALCSDYCFNSGPFLSLEWFDRFITPNLIREIKAYREMGFYTIKHTDGN
                     IMPILDRLLAATPDAIHSLDPQGGIDMAEIVARAGDKVTLIGNVDCGKLQTGTDEECI
                     ESATYAIENGRKAAGYIFSTSNCIYTGMELRRYELIRNVWKKHRNY"
BASE COUNT          969 a          992 c         1100 g         1079 t
ORIGIN      
        1 atcgggcgat ttgcgtctga tatcgagcgg gcctgaagcc ggcttgggtg aaatcagacg
       61 caaatcgccc gatacctaga agattgcttg ccagcgcttt gagtatgccg tgaacttcca
      121 cgaatacgtc ggcattctgc gtggcgtcta tcatgttttc cgcgcgggcc aggcccaacg
      181 aggtgaccgc ccgaagattc tcgatagcct tgaatatgaa ctcacgcggc gcgcctatgc
      241 ccgtgccgat ggctgtgccg cccagattca ccaccctcag ccgctcgacg cacttgtaaa
      301 tccgccagcg gtcgcgggcg aacgcttcgg cgtatgcgct catctcacgg cccagcgtgg
      361 tcagcacggc gtcctggtat tgcgttcggc cgactttgac gacgtcgtcg aaaagttttt
      421 ccttcgcctg gaattcttcc agaagtgcca cgacggctcg ttcgagtccg ccgagttcaa
      481 cgatggctgc caccttcaat gccgtaggat atgtatcgtt cgtggattga tgcagattca
      541 catggtcgct tgggctgatg aattcataac agcctggttc gtgtcccatg atttgcagcg
      601 cacggttggc cagcacttcg tttacgttca tgttcgtgct tgtgccggcg ccgccctgga
      661 gtgcgtcgac tattatgtgt gcgtcgagac agccgtccgc catttcgcgg caggcttcag
      721 agagtgcggc gtattgttcg tcgctcaggc agtcgatgtt gtgattcgtc atgacgcagg
      781 ccagcttgac tgcgccgtat gcgcgtatca gcggcggcct gaccggatgg ccggctaacg
      841 acgaaaaatt ctccgccgca cgcagcgtgt gtatgcccca caatgcgccg gcaggcagtt
      901 cgcgagagcc cagcatgtca tgttcagtac gtctgtcagc cattttttta aaaaatcctg
      961 tatcagcaaa atgaaaaatc atgttatccg gttttattat agaagtatat cgaaagtttt
     1021 tccagatttt acgactttgg taaggtttcc acgataacgc atcaggctga actttcaact
     1081 ttaatgtgtt tcagccggaa aatgggtttg acatgttttg gacggaggtg tatcatctgc
     1141 atctcaatgt ggaatcggac gtgcggtata ggactgcgcg ttaatcggta gtttttttat
     1201 gtttttacag gtatctcaaa ggagaaccac aaatggcagt gaaaataggt atcaacggtt
     1261 ttggtcgtat cggccgcctt gtattccgcg tgatggccga acagccggaa cgttttgaca
     1321 tcgtaggcat caacgacctc ggcaaggcag aattgctggc gtatctggcg aaatatgact
     1381 ccactcaggg ccgttttcct ggcaaaattg ttgccaagca gaacgccctt gtcgtgaacg
     1441 gcaaggaaat tccggtattt tccgaaaaag acccggctaa cctgccctgg ggcaagttgg
     1501 gcgtggatgt cgttatagag tcgacgggca ttttcaccaa tgccaaagcc gacggcaaag
     1561 ccggttatga cagccatctt gacgcaggcg ccaagaaggt tatcatctcc gctccggcga
     1621 agggttttgc ctccaccgtg gtcatgggcg taaacgatga tatcatcact cccgaagtca
     1681 cctgcgtttc caacgcgtcg tgcaccacga actcgctggc tccggtggtg aaggttcttc
     1741 acgagaaatt cggcgttgta aaaggtctga tggttacctg ccacggctat accaacgacc
     1801 agcatgttct cgaccagatt cacaaggatc cgcatcgtgc gcgtgcagcc gccatcaaca
     1861 ttatccccac aagcaccggc gcagccgttg ccgtgggcaa ggttctgccg gaactcaacg
     1921 gcaaactgga cggctactcg ctccgagtac cggttccgac aggctctatt accgacctga
     1981 ccgtcgaatt cgccaaggac gttgacgttg aaagcatcaa tgaagccgtc aaagccgccg
     2041 ctgaaggcga aatgaagggc atcatcgaat ataacaccga cccgatagtt tcgagcgaca
     2101 ttattcacaa tagtcacagc tcgattttcg acgccggcaa taccagggtt atcggcggta
     2161 acatggctaa ggttacgatg tggtatgata acgagtgggg ctactcgaac cgcaccgcag
     2221 acctggctga aaagcttgcc atcctgtaag cctgttttta caagtgagcc aaaaaagccg
     2281 ccgcggcata acaaccgcga cggctttttt aatttattca aactgaccga tttaatggtt
     2341 tccagttaca gagtactagg tgtaaaggta aatacttgtt tcgcatcagg ttaaaatcat
     2401 tatgaaagat aagatgaata tatggcaggg taagcataaa attccgtggg acgattatga
     2461 tttcagccgc cgcatgttag ctgaacattt gacgcaggaa catgatatgg ctagccgcag
     2521 gattgaatgg atagagcggc aggtgacatg gattcataaa aatctttttg atgggcagcc
     2581 gtcgaaaatt atggatttgg gatgtggccc agggttgtat ctgcatcggt tggtaaaact
     2641 cggacatata tgttgcggcg tcgattttag ccctgcgtcc atagaatatg caaacctgca
     2701 caatcccgat agagtgaatt gcatattttt caagagtgat attcgcgaaa ttacgtttgg
     2761 tgtggattat gatttgataa tgatgctttt tggagaattt aatacttttt ctccggctga
     2821 ggcattgtcg attctcagga agtcgtgcga tagcctgcat aatcatggcg gccgtatgat
     2881 attggaaatt tcgacgccgc aggcggtaag tcgtattgga cgaagtgaat catcgcggga
     2941 ggaggccgag tcgggactat tttcagacga gccttatgta tgtgaaacca gcaaccgatg
     3001 gctgccggaa tataatgctg cagttcagac tttccgtgtt gaagagaagt caacagaaaa
     3061 aacgacgatt tatcgaagta ctacccgtgc gttatcggat gatgaattaa gggaattgat
     3121 aatgagcgca ggatttgcca gtgcgacgag gcttagagaa tggccttgta atacagacga
     3181 tttgcagtta tggctgattg agaagtgata actaaagtat cctccgtcgg gcggataggc
     3241 gaaagcctga taaataggat gttacggttt taatccgtag agattcgctt gcttcctcaa
     3301 agaataccgc accgggtatc tcttttatat aaagcatgtc tgcgttcagt aatttctgtg
     3361 ttttttccac acattgcgga taagttcata tcggcgaagt tccatgccgg tgtatatgca
     3421 attactggtc gaaaaaatat agccggcggc tttcctgccg ttttcgattg catacgtggc
     3481 tgattcgatg cattcctcgt ccgtgccggt ctgaagtttg ccgcagtcca cattgccgat
     3541 tagcgtaacc ttgtcgcctg cgcgggcgac tatttccgcc atgtcgattc cgccctgcgg
     3601 gtcgagcgaa tggatggcgt cgggggttgc agccagcagg cggtcgagta tcggcatgat
     3661 attgccgtcg gtgtgtttga tggtataaaa acccatctcg cggtaagcct ttatttcgcg
     3721 gatgagattc ggcgttatga acctgtcaaa ccattccagc gacaggaatg gccctgagtt
     3781 gaagcagtaa tcgctgcata acgcaaatgc gtccatgccg ccgtgggttt tgattttttc
     3841 ggctcgcacg attgcgtcgt caactctttg ggcgagtttg gcgtcgactt tctccggctc
     3901 gtcaatcatg cggaacgacc attcttccat tttgccgccg tcaggtattg cgaatgtcgc
     3961 atcgccgtgg cacatgagaa aataatcgtc gccgctcatc gccttgattt catccacgat
     4021 ttgcatcagg atttccactt cgccaggatt ttcgtggatg aatatggcat catgttcaaa
     4081 tttctgtgcg gtgtttacgt aaatggcggc catatccttt atatgcagcc gttgttcagt
//
LOCUS       DAONFI010000204         4136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_25944_length_4136_cov_2.895614, whole genome shotgun sequence.
ACCESSION   DAONFI010000204 DAONFI010000000
VERSION     DAONFI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25944_length_4136_cov_2.895614"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLK08_07095"
     CDS             complement(<1..391)
                     /locus_tag="PLK08_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPS53300.1"
                     /translation="MLKQLLEKIKKREVVVGVMGLGYVGLPLVREFIRGGANVLGFDL
                     DERKIKSLMAGKSYIEHIPSATVQEMLAGGRFSATSNPKQLAKADCILIAVPTPLNKM
                     REPDLQYVEATAIPFLRLSAKASLSFWN"
     gene            complement(510..1484)
                     /locus_tag="PLK08_07100"
     CDS             complement(510..1484)
                     /locus_tag="PLK08_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53301.1"
                     /translation="MSGRLYVSPSSSSSVNIGRGVSFGSSNTKYTPKTIGDLKPTDQG
                     IYKPGSDYNNMIRGMKQGMAFNVGKYEITAVGDIGWDRKDTLESQLTVGKKYGIRNDA
                     SIARSNAYVEALAGSGYVKESTLDVNADSSHITTLVPKNGGEFAELIKRGEDDFKREL
                     YQESLSLFKLARRISPRSPEVLLSLFHASFAASSGLSYSLPSYYLQLSLKYFPELPLV
                     DVHPKYFYGREGDFMRDQVRLEQYLESQNRDSQAWFILGYIAWRNGQTAEACKYLQNA
                     YDLSQDSELKEAVSIMWDGMRASGKVKDKLIIIIPQKSKTPVSQPSGK"
     gene            complement(1936..2451)
                     /gene="pyrE"
                     /locus_tag="PLK08_07105"
     CDS             complement(1936..2451)
                     /gene="pyrE"
                     /locus_tag="PLK08_07105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPS53302.1"
                     /translation="MDKAELARRVKEVALLEGDFVLRSGKRSKYYLDKYLFSSCPEIL
                     AELGRRFSEYAADVDMIAGPELGAVSLAAATSMASGKPFAIVRKGKKGYGTDKLLEGP
                     DVRGKRILLVEDVGTTAGAALEAAEVLRQAGAIIQRLVFAINRQEGAQENVENAGYEF
                     GYILSKDDLGI"
     gene            2687..3487
                     /locus_tag="PLK08_07110"
     CDS             2687..3487
                     /locus_tag="PLK08_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPS53303.1"
                     /translation="MKKRILLLLALPVIVCVAFAITMHHNAMAASDSQYIKTGYSTHK
                     FAEVKSLKLNSNYLLFLPEDYGKPDKKFPVILFLHGSGERGCNPEKLKEFGPFTQAAK
                     ESSFPFIVIGPVCPAGRWWSDAEVSLSVMALLEKICKDYAVDRDRIYLTGLSMGGFGT
                     WSLAEQYPNVFAAIAPVSGGGNPYRANEIKHIPTMIFHGKNDRNVPVWQAQQMAMSLT
                     QAGGDAKLLIYPNIGHNIWGLVYSRPQLYSWFMEHTKSAPNTPATPAP"
     gene            complement(3682..>4136)
                     /locus_tag="PLK08_07115"
     CDS             complement(3682..>4136)
                     /locus_tag="PLK08_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS53304.1"
                     /translation="VEALLDFFLERFAAEYGQSVRYIAPEIRETLRTYGWPGNVRELS
                     CWVERLYAANLPPVPPTNEMWDDQYVYAAVPAQPAFAEAQPAFSFAASPATVSAAAPA
                     ATNASLEQAETETIRSVLAQCDGNRSRAADMLNIHRTTLIRKIKQYGL"
BASE COUNT         1051 a         1107 c          922 g         1056 t
ORIGIN      
        1 gattccagaa cgataagctg gcctttgcgg ataatcgtag aaatggtatt gccgtcgctt
       61 caacatattg caaatccggc tcgcgcatct tgttcagcgg cgtagggacg gctatgagta
      121 tgcagtcagc cttggcgagc tgtttgggat tgctggtggc agagaacctg ccgccggcca
      181 gcatttcctg aaccgtcgct gacggaatat gttcgatata actcttgcca gccatcaggg
      241 atttgatttt ccgctcgtcc aaatcaaagc ccagaacatt agcaccgccg cgtatgaatt
      301 ccctgaccag cggcaggcca acgtaaccca gtcccatgac gccgacgacg acctcacgtt
      361 tttttatctt ttccaaaagc tgtttcaaca ttctttgtct gtctccaata aaagaaagtc
      421 tgcccgtaac attacaagtg cagacctgta ttaaagtaag aaactgatat tttccaaaaa
      481 aaaacggaat tgtccagcca aaagtcgccc tattttccag aaggctgact gactggcgtt
      541 ttggatttct gtgggattat tatgataagt ttgtccttca ctttgccgct ggctctcatg
      601 ccatcccaca taatggagac cgcttctttc aattctgaat cctggctgag gtcgtaggca
      661 ttctgtaaat atttgcacgc ctcagccgtt tggccgtttc gccatgcaat atatccgaga
      721 ataaaccatg cctgtgaatc gcgattctga gactccagat attgttcaag tctgacctga
      781 tcccgcatga agtcaccttc acggccgtaa aaatatttcg gatggacgtc taccagcggc
      841 agttccggaa aatatttgag cgataattgc aaataatatg acggcagaga gtacgacaac
      901 cccgacgacg cggcaaaact tgcatgaaac aggctcagaa gcacttccgg acttcgaggc
      961 gatatgcgtc gcgcgagctt gaacagactc agcgattcct ggtaaagttc tctcttgaaa
     1021 tcatcctcgc cgcgttttat cagttccgca aactcgccgc cgtttttggg cacgagcgtg
     1081 gttatatgcg acgaatctgc gttaacgtca agtgtcgatt cttttacgta gccgctgccg
     1141 gcaagcgcct ctacatacgc attcgaacgg gcaatactgg catcgttcct gatgccgtat
     1201 ttcttgccga cggtcaactg gctctcaagc gtatccttcc tgtcccagcc gatgtctcct
     1261 accgccgtaa tttcatattt tcccacatta aacgccatgc cctgcttcat tccgcgaatc
     1321 atgttgttat aatccgaacc aggtttataa attccctggt cggtcggctt gaggtcacct
     1381 atggttttgg gggtatattt cgtgttgctc gacccgaacg atacacctct gcctatattt
     1441 accgacgagc ttgatgatgg gctgacgtaa agtctacccg acattccacc gacatttgag
     1501 gaacgtccgc cagacgacgg accagcatac gaaccgactc cggttcgatt catatcggaa
     1561 tcgtattgaa gggagcgatt aagcaggccg gttgaactac ccaagcctcc caaaccccaa
     1621 gaaccgccca tttgcgacct tttatcggca tctgtagtta ttctcagacc ttccccaaag
     1681 gcgactcccg ccatgataag gataatcgca accatacata aaactggtct gaaaacgcct
     1741 cgtaacatct aaaaataccg cttcctaata taacataaca ccatagctta gcgctgttcc
     1801 catggaggtt cattctagag aattcattat atcaaaataa agcaaaaacc ccaatttatt
     1861 ataaaaaaat accctcttat cacaaagaag gtatttaaac gtcaatatta aggaattcag
     1921 gaacagggtt tgtttttata ttccaaggtc gtctttgctg agaatgtacc cgaattcgta
     1981 gccggcattt tccacatttt cctgcgcgcc ttcctgccga tttatggcga aaaccagccg
     2041 ttgaataatc gcgcctgcct gcctgagaac ctcagccgcc tccaatgccg ccccagccgt
     2101 agtccccaca tcttccacca gcagaatgcg tttgcccctg acatccggcc cttccaggag
     2161 tttatccgta ccgtaacctt ttttgccctt gcggactatt gcaaaaggct tgccgctggc
     2221 catcgacgtg gcagccgcca atgataccgc acccaattca gggccggcta tcatatcaac
     2281 gtcggctgcg tattccgaga atcgtcggcc aagctccgcc aatatttcgg gacagctgga
     2341 aaacagatat ttatccagat aatatttgct gcgtttgcca ctgcgaagaa cgaaatcgcc
     2401 ctccagcaac gccacttcct tgaccctgcg ggccagttcc gccttatcca tgaaaatttc
     2461 tccaatagca attagccgta taaattccgc ctgcatgcac acaggcaaaa acgatatgga
     2521 gttcctatca ttatccggct ctcaagtcaa tgttttttgg caatttcgcc aaaggccagc
     2581 cgaatcggac gaacacaatt taaaaacagc ttcctataat ttcttttgat tattcgctct
     2641 taagaattta ttttataatc ataagttgtt taaaggagac acgcgaatga aaaaacggat
     2701 actgttattg cttgctctgc cggttattgt ctgcgtcgct ttcgcgatta ccatgcatca
     2761 taacgccatg gcagcctccg attcgcagta catcaaaaca ggctactcga cacataagtt
     2821 cgcagaagtc aaatcattaa agcttaacag caactatctg ctttttctgc cggaagatta
     2881 cggcaagccg gataaaaaat ttccagtgat tctgtttttg cacggttcag gcgagcgcgg
     2941 ctgcaatcct gagaaactca aggaattcgg gccgttcacg caggcggcaa aagagtcgtc
     3001 gttcccgttt atcgtaattg gcccggtctg cccagccggc cgatggtggt cggatgcgga
     3061 ggtgagtctc tcggtaatgg cactgctgga aaaaatatgc aaggattatg cagtcgaccg
     3121 tgaccgtatt tatctcacgg gcttgagtat gggcggcttc ggcacatgga gtcttgcgga
     3181 gcaatatcca aacgtatttg cagctatcgc accggtaagc ggcggaggca atccgtatcg
     3241 ggctaatgaa ataaaacaca ttccaaccat gatttttcat ggcaaaaacg accggaacgt
     3301 gcccgtctgg caggcacagc agatggccat gtctctcacg caagccggcg gcgatgccaa
     3361 gctgctgata tatcccaaca tcggccataa tatatgggga ctggtatatt cacggccgca
     3421 gttgtactcg tggtttatgg agcatacgaa atccgcgcca aatactcccg caacacctgc
     3481 cccatgattc accgttccgc cggatatatc cctgtacaat ataaagacac aaaaaaaccg
     3541 tacccttagt atctggtacg gtttttattt tagagttatt ttcaaatctg tacagactct
     3601 ccatgcatgc ctgactttta acagccagac gcccttcagg tctgcatcca ccatccgcaa
     3661 ctttcagtat cgacctactt tttataagcc gtactgtttt atcttgcgga tgagcgtagt
     3721 cctgtggatg ttcagcatat cagccgcccg cgaacgattt ccgtcacatt gtgcaagtac
     3781 ggaacgaatg gtttccgttt ccgcctgttc caatgaagcg ttcgtcgccg caggagctgc
     3841 cgccgaaacc gttgccggtg aagcagcgaa agagaatgcc ggttgtgctt ccgcaaatgc
     3901 cggctgagcc ggaacggccg cataaacgta ctggtcgtcc cacatctcat tcgtcggcgg
     3961 tactggcgga agattggctg catacagccg ttcaacccag caactcagct cgcggacatt
     4021 gcccggccaa ccgtaagtac gcaacgtctc gcggatttcc ggagcgatgt acctgacgga
     4081 ttggccgtac tcggcggcaa agcgttcgag gaagaaatca agcaatgctt cgacat
//
LOCUS       DAONFI010000205         4123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_26063_length_4123_cov_4.215339, whole genome shotgun sequence.
ACCESSION   DAONFI010000205 DAONFI010000000
VERSION     DAONFI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26063_length_4123_cov_4.215339"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="murD"
                     /locus_tag="PLK08_07120"
     CDS             <1..816
                     /gene="murD"
                     /locus_tag="PLK08_07120"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPS53305.1"
                     /translation="IVVLELSSFQLEDLPIVGVSPCVALVTNLAANHLDRHGTMLAYG
                     QAKKNIFRFQHDGDVAIFNSLDREVAAWADEAPANADFFPIKGADFSLTVPGGHNQLN
                     AQAAWAIAKQFGITKKLAAAALKDFKGLPHRLAFVCEKRGIRYFNDSKSTTPAGACVA
                     LNSFDKGRVVILLGGYDKGADFTDMARLTAARAKAAVTFGKTGSAIYAAINGCLNHCE
                     SVNCDNLMQAVEHARKFARKGDVILLSPACASYDQFDNYERRGELFVKLAQQD"
     gene            complement(890..1717)
                     /locus_tag="PLK08_07125"
     CDS             complement(890..1717)
                     /locus_tag="PLK08_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPS53306.1"
                     /translation="MQYLWLTLLGLGVGAFGTLIGAGGGFILMPILLLAYPDGDPAVL
                     TSISLAMVCCNASSGSYSYARMKRVDFKSGLLFLLTGIPGSIAGAYVVNYIPRHMFNF
                     VFGVLLLAGGAFIFYRTFREDKRKHETRPTFMRDFVDAGGIRYTYGYSLRVGMTLSFF
                     VGVASSILGIGGGIIHVPAMVNLLNFPVHIATATSHFILSLMSLAGTAVHVIDGSLGI
                     KELPCVLPLGLGAVIGAKLGAKLSKRIHGRWILRSLAGALGLVGIRILILAIVSMMH"
     gene            1962..3557
                     /gene="murJ"
                     /locus_tag="PLK08_07130"
     CDS             1962..3557
                     /gene="murJ"
                     /locus_tag="PLK08_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPS53307.1"
                     /translation="MTAKEQDKFFSAARMVAFLTLASRILGLIRDMIMVPLGSPVMAD
                     RFWTAFGIPNLFRRLFGEGALSSAFVPVYTEVCEAKGMNRARLVLANVAGILAIILCC
                     IILLVSLGLWAWWEICGGDYYRLVLLQFTGIMLPFMFTICMLALGSAALNCRGHFAYP
                     AFAPLILNVGMIITAKCIAPAICKTDIGQFYVYCVSVVVSGVVQLVGVVWLLKKSGLA
                     ALANFRPILPETKEIGRLMLPMLVPISILQFSAFADWLIALKFTATPDSPNLPLTDGT
                     VRCLYAANRLYQMPMGVLAISIATVVFPLFSRYASRNDRLGLKDATNRALRLSLFLGI
                     PSGLALIIMARPIIELLFQRKNFLESDTIRTAHMLQAYSVGMWAYFCNHILLRAFYAI
                     KQPRKPLQIACGLVCLNLALVICGIFTPLKGSAIGLATAITSSANTVLLIWFLRKQVG
                     LIGFRKILKSLAKITVASSVMVLGILAAMYGTEQIPLQHHVTLSGIIKVVAGVAGGMA
                     CYFITAFALKSPELPEIIKRRAA"
     gene            complement(3600..>4123)
                     /locus_tag="PLK08_07135"
     CDS             complement(3600..>4123)
                     /locus_tag="PLK08_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53308.1"
                     /translation="GTFLLNFGGGGIGSRGIVGVLGACLFYSLSDMNITILVKAVEAQ
                     HFTRWNSVLFSVCTSYILCGLICGPLIFFVKNFRRNDFKDSIGFAFFWLAAMVFLYAT
                     FSIVGTVYGNILQSTRGIISIILGVIIAGMGHHHIEAHMTRPVFIRRLIAGVIMTFAI
                     IAFGWNQLMQYIS"
BASE COUNT         1024 a         1053 c         1087 g          959 t
ORIGIN      
        1 atagtcgttc tggagctttc gagttttcag cttgaagacc tgccgatagt cggcgtaagt
       61 ccttgcgtgg cgttggtgac gaatctggcg gctaatcatc tcgacaggca cggcacgatg
      121 ctggcatacg gccaagccaa gaaaaatatc ttccgcttcc agcatgacgg cgacgtggcg
      181 atattcaact cgctcgacag agaagttgcg gcgtgggcag acgaagcgcc ggcaaatgcg
      241 gactttttcc ccataaaagg tgcggatttc agtttaaccg ttccaggcgg gcacaatcag
      301 ttgaatgcac aggcggcgtg ggctatcgcg aagcagttcg gcattacgaa aaagttggcg
      361 gctgcggcgt tgaaagattt taaaggcctg ccgcacaggc tggcttttgt ctgcgaaaaa
      421 cgcggcataa gatatttcaa tgattccaag agcactacgc cggctggcgc gtgcgtggcg
      481 ttaaattcgt tcgataaggg cagagtcgtc atacttcttg gcggctacga caagggtgcg
      541 gactttaccg acatggcacg gctgacggct gctcgcgcaa aagccgccgt aacattcggc
      601 aaaaccggtt ctgccatcta tgccgccata aacggctgtc tcaaccactg cgaatcggtc
      661 aactgcgaca atctcatgca agccgtcgag cacgccagaa aattcgcacg gaaaggcgac
      721 gtcatactat tgagcccggc ctgtgccagc tacgaccagt tcgataatta cgaacgtcgc
      781 ggcgaacttt tcgtcaaatt ggcacagcag gattgacgca tatcaagttt aagttacccg
      841 caccaggttt aattccacca gctttagctg gtagaaattt taaaatgctc taatgcatca
      901 tcgagacaat cgccagaatc agtatgcgaa tgccgacaag gcccagtgca ccagccaggc
      961 tgcgcagaat ccagcggccg tgaatgcgtt ttgaaagttt ggcgcccagt ttcgcaccga
     1021 taacggctcc gaggcccagc ggcagaacgc acggaagctc cttaatgccg agtgagccgt
     1081 caataacgtg taccgccgtg ccggctaacg acatcagcga caaaatgaaa tggctggtag
     1141 ccgtagctat gtgcaccgga aaattcagca gattcaccat cgctggaacg tggataattc
     1201 cgccgccgat gccgagtatg cttgatgcga ctccgacgaa gaaactcagc gtcatgccca
     1261 cccgaaggct gtagccgtag gtataacgaa tgccgcctgc atctacgaaa tctcgcataa
     1321 acgtcggcct tgtttcgtgc ttgcgtttat cctcgcgaaa cgtgcgataa aaaataaacg
     1381 caccgccagc cagcaaaagc actccgaaaa cgaaattgaa catatgccgc ggaatataat
     1441 tcaccacata cgcccccgca atagaacctg gaatgcccgt caacagaaac agcaaccctg
     1501 acttgaaatc cacccgtttc atgcgggcgt atgaatacga gccgctggat gcgttacagc
     1561 acaccatcgc cagcgaaatc gacgtaagta cggcagggtc gccatcggga taggctaaaa
     1621 gcagtatggg cataagtatg aacccgccgc cagcgcctat gagcgtgccg aacgcaccta
     1681 cgccgaggcc cagcaatgtc agccataagt attgcatcgc aaacgagttc ctttccagtg
     1741 ctaatgccgt aaatcagccg ccacggttgt gattgtagtt acttgcagtg aataaaaaaa
     1801 ggactatttg ccgataattc caatgcagag acacaactcg acaaaaattt tcgggcacac
     1861 taaggagtgc ttccggaagt atttgtagat atgttgtctg gttaaaacgt ttttatctga
     1921 gacaattaga agctgtttcc aaatatcttc cgagatttca tatgaccgca aaagagcagg
     1981 acaagttttt ctccgccgcc aggatggtgg cgtttctgac gttagcatcc cgcatacttg
     2041 gtctgatacg cgacatgatt atggttccgc tgggttcgcc ggtgatggca gaccggttct
     2101 ggacggcgtt cgggataccg aatctttttc gtcggctatt tggcgaaggt gcgttatcgt
     2161 cggcgtttgt gccggtttat accgaggtat gcgaagccaa aggcatgaat cgcgcaaggc
     2221 ttgtcctggc taacgtcgca ggcatacttg cgataattct gtgctgtata attttgctgg
     2281 tttcgctggg gctatgggcg tggtgggaga tttgcggcgg agattactac cggctggttc
     2341 ttttgcagtt caccggcata atgctgccgt tcatgttcac catctgtatg cttgcattgg
     2401 ggtcggcggc gttgaactgc cgcggccatt ttgcgtatcc tgcatttgcg ccgctgatac
     2461 tgaacgtcgg catgataatc acagccaaat gcatcgcgcc tgcgatatgc aaaaccgata
     2521 tcggccaatt ttacgtttac tgcgtttcgg tggtggtctc cggcgtggta cagcttgtag
     2581 gcgttgtgtg gttgttaaag aaaagcgggc tggccgcgct ggcgaatttc cggccgatac
     2641 tgcctgagac gaaagaaatc ggcagactca tgctgccgat gctcgtgccg ataagcatat
     2701 tgcaattcag cgcgtttgcc gactggctga tagcgcttaa atttaccgcc actcccgact
     2761 cgccgaatct gccgctgact gacggcacgg tacggtgtct ctacgcagcc aaccggctgt
     2821 accagatgcc gatgggcgtg ctggcgatat ccatagcgac ggtggttttt ccgctgttca
     2881 gccgctatgc gtcccgcaac gacaggctag gcctcaaaga cgcaacaaat cgcgcattgc
     2941 ggctgagcct ttttctgggc ataccgtcag gcctggcgct catcattatg gcaaggccga
     3001 taatagaact gctttttcag cgtaaaaatt ttctggaatc cgataccatc cgcacggcgc
     3061 acatgcttca ggcatacagc gtcggcatgt gggcgtattt ctgcaaccat atccttctgc
     3121 gggcttttta tgctataaaa cagccccgca aacctcttca gatagcatgc ggcctggttt
     3181 gtctgaatct ggcgttggta atatgcggaa tattcacgcc actcaaaggc tcagccatcg
     3241 gccttgcgac ggcaataacg tcgtcggcaa acacggtact tctgatatgg tttctgcgca
     3301 aacaggtcgg gctgataggt tttcgtaaaa tactcaaatc gctggctaaa attacggtcg
     3361 cgtcttccgt catggtgctg ggaatattgg ctgccatgta cggcacggaa cagatacctt
     3421 tgcaacatca tgtgacgctc agcggcataa taaaggtagt tgccggcgtg gctggcggga
     3481 tggcctgcta tttcattacg gcatttgcgt taaaaagtcc tgagttacct gaaattatca
     3541 agagacgcgc cgcataaaag agctaaagcg gttaaaaagt cctgaactgc ctgagattat
     3601 caagagatgt actgcataag ctgattccag ccgaatgcga ttatggcaaa tgtcattatt
     3661 acgcccgcaa tcagccgccg gataaaaacg ggtctggtca tgtgcgcttc tatgtgatga
     3721 tggcccatgc cggcgattat cacaccaagg atgatagata ttattccgcg agtcgactgc
     3781 aggatattgc cgtacaccgt gccgacgatt gaaaatgtgg cgtacaggaa taccatagct
     3841 gccagccaga aaaatgcgaa tccaatcgaa tctttaaaat cgtttcgcct gaagtttttg
     3901 acgaaaaata tcagcggccc gcagattaga ccgcaaagga tgtatgaagt gcacacgctg
     3961 aaaagtacgg agttccacct tgtgaaatgc tgtgcttcca cggccttgac cagaatggtt
     4021 atgttcatgt cggaaagcga gtagaacagg caggctccca gtacgcccac aatgccgcgg
     4081 ctgccgatgc cgcctccgcc gaaattgagc agaaacgtac ctg
//
LOCUS       DAONFI010000206         4102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_26257_length_4102_cov_6.813442, whole genome shotgun sequence.
ACCESSION   DAONFI010000206 DAONFI010000000
VERSION     DAONFI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26257_length_4102_cov_6.813442"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLK08_07140"
     CDS             <1..392
                     /locus_tag="PLK08_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53309.1"
                     /translation="NTTDFYDVYTASGTGVTNWDDATATWNSNQPTWTYTLAENDEVN
                     ITAAGWYHWDITTSLSRTDVVTFGLLSKVETSDTNQRAFFYSTHGGTSTQVQPYLEVT
                     YEAVPEPTTAMLLLGIGCVRVFCKNGF"
     gene            complement(784..1923)
                     /locus_tag="PLK08_07145"
     CDS             complement(784..1923)
                     /locus_tag="PLK08_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS53310.1"
                     /translation="MSKSKKLIWTSHSDLAINRRPNVGRREYLDYMTFTANERPLFTE
                     LFGPMVGLKENWKEQGATEAELDFSAFRYRCETSAGISANTGWLGGEDVVLSDNSEYK
                     IIRDAKGRTVKLPKGYATLGLPQDFPVKTMDDWLAVKKHYLFDESRLMDGWYERAMDC
                     IRRDNVMVIGIPGGYDELRELMGDELACISFYDQPELIEDLLQTIGDTALKVLERVSE
                     KIQIDVLSVHEDFAGKNGPLVGPEHFRQFIAPYYRRIWDFVASKGARLFDIDSDGNID
                     AIIPALLDAGINCIHPFESAAGMDIVKVREQYGDRLAICGGLDKFVIAKGKDEIVREL
                     EYKLPPLIATGGCIFGLDHRIPENTSLENYKFYLEKVWQIFENHN"
     gene            1999..2823
                     /locus_tag="PLK08_07150"
     CDS             1999..2823
                     /locus_tag="PLK08_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53311.1"
                     /translation="MDYSCKNMYYYDMDISPLKIRINNTLLTYCDSKWHWDVSPGGFR
                     DFDLWYVCRGKGFMNLDGNDKKVSDGDCLFLRPDMSIHGTHDPQWPLVVVSVHFDFIG
                     KNGQTIMPASQNLPAKIQNMQESHFFEVLLRRTYDFFSNNRKDAATKYFSVAVHEFFY
                     QETKFQFPPEQERAIDDICRQIIRDPGKRSSVSDLAQTTGYSVDHFSRLFRTIKGTSP
                     KEFIVRARIQAAKGFLKNSNHSIAHIAEILGYNDQFFFSKQFHSWTGQSPRQFRLL"
     gene            complement(2856..3686)
                     /locus_tag="PLK08_07155"
     CDS             complement(2856..3686)
                     /locus_tag="PLK08_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53312.1"
                     /translation="MAHADENPLLPCYGVLAASHSHTRDFRTALHAHPFYSLFYIVAG
                     KGFLDINTAAESKRIELSDNSAVLLEPGINHQIIDRPRNPMTLFVIYFSCQTADGITS
                     PDIAALRQTPVKPGGFTAAEIRKQLRKILNEQSQQLIGYEASVISSLNAILISLLRTS
                     PVNMQNGASAQKRVRAILEYIQHNFSQSYSLSDAAAEINLSERHFTNLCRKITGMSFR
                     KYLEGLRLSRARELLETTNMPATAIAFEVGYEELSTFYRAFKAKFKFSPIAYKKLIER
                     "
     gene            3780..>4102
                     /locus_tag="PLK08_07160"
     CDS             3780..>4102
                     /locus_tag="PLK08_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPS53313.1"
                     /translation="MSDKHEQYGKFPASLDELTKLAADIDVVLPVSRHIELLAEGITV
                     DGFKLPNRLAIHPMEGCDGEFDGRPGELTLRRYHRFAGGGAGLIWGEAIAVVPEGRAN
                     PRQLH"
BASE COUNT         1089 a          909 c          941 g         1163 t
ORIGIN      
        1 aaaatacgac tgatttttat gatgtttata ccgcttccgg cacaggggtg acgaactggg
       61 atgatgctac ggctacctgg aacagtaacc agccgacctg gacatatacg cttgcggaga
      121 atgatgaagt taacattact gcggcaggct ggtatcactg ggatattacc acaagtcttt
      181 ccagaaccga tgtcgtgaca ttcggccttc tgtccaaggt tgagacatct gatactaacc
      241 agagagcttt tttctattca acccacggcg gcacatctac ccaggtacaa ccttatctgg
      301 aagtgactta tgaagctgtt cccgaaccga cgaccgcaat gttgttgctc ggcatcggat
      361 gcgtcagagt attttgtaag aatggttttt aaaaatagtt ttatgtcgtt gccgagtcgg
      421 aactcggaag taacaagaat caagaaacaa tttacaagta aactttcaag tatcgtataa
      481 ctgttagagc attgaaaaat tgagagttca tatttttgcg tcagtgggtt tgttcgtggc
      541 aaggaaggaa aatgaggcat acttggcagt attctgaagt ttttctgacg cagccacgga
      601 caaagcaaac cagcaaaaaa ggaactctca agattgaact gctctaatct aaaaaacttg
      661 aaaatagtta ctttgaagtt gcttgtaatt tgttttcttg ttctttgaaa cctatttccg
      721 ttcagcctga catttattat gtttcttaat cggaaaattt tcggaaagat gtttgaaaac
      781 tgctcaatta tgattttcaa atatctgcca gactttttcg agatagaatt tataattttc
      841 caaagaagta ttttccggta ttctgtgatc aaggccgaat atgcagccgc cggtcgcaat
      901 aagcggcggc agcttatatt caagttccct gactatttca tccttgccct tggcgatgac
      961 gaatttatcc agtccgccgc agatggctag tctgtcgcca tattgttcgc gaaccttgac
     1021 gatatccatg ccggcggctg attcaaatgg atggatgcag ttaatgccgg cgtcaagcaa
     1081 cgcgggaatg attgcgtcta tattgccgtc gctgtcgatg tcgaacagcc ttgcgccttt
     1141 tgacgcgaca aaatcccata ttctccggta atatggtgct ataaattggc gaaaatgttc
     1201 cgggcctaca agcgggccgt ttttgcctgc gaaatcttca tgaaccgaca aaacgtctat
     1261 ctgtattttt tcgctgacgc gttcaagaac ctttagtgcg gtatcgccta tcgtttgaag
     1321 gaggtcttct atcaattccg gctggtcata aaaacttatg catgcaagtt catcgcccat
     1381 aagttcccgc agttcatcgt agccgccagg aattcctata accattacat tatcacgcct
     1441 gatgcaatcc atggctcgtt cataccatcc gtccatcagt ctggattcat cgaacaggta
     1501 atgctttttt accgccagcc agtcatccat ggtttttaca ggaaaatcct gcggcaggcc
     1561 cagcgtcgca taaccctttg gcagcttgac ggttcggcct ttggcgtcac gaatgatttt
     1621 atattccgaa ttgtcactta atactacgtc ttcgccgccg agccagccgg tatttgcaga
     1681 tattccggct gatgtctcac agcgatatcg aaatgccgaa aaatccaatt cagcctcagt
     1741 cgctccctgt tccttccaat tttctttcag gcccaccatc gggccgaaaa gctccgtaaa
     1801 caaaggcctt tcgttggcgg taaaagtcat atagtcaaga tattctcgac ggcctacgtt
     1861 gggacggcga tttatggcca agtcactgtg cgaagtccat atcagttttt ttgatttgct
     1921 catttttcat atcctgtact attacgatat tactcttttc aataaatttt aatatacgcc
     1981 ttatatcgag gttttgcaat ggactattcc tgcaaaaata tgtactatta cgacatggat
     2041 atatcgccgt tgaaaatcag aatcaacaat acgcttctta catattgcga cagtaaatgg
     2101 cattgggacg tatcgcctgg aggattccgt gattttgatc tctggtatgt ctgccgcggg
     2161 aaaggtttta tgaatcttga cggcaatgac aaaaaagttt ccgatggcga ttgtctgttt
     2221 ctcagacccg atatgagtat acatggcacg catgacccgc agtggcctct tgtcgtcgtt
     2281 tcggttcatt tcgattttat cgggaaaaac gggcagacta ttatgccggc cagtcagaac
     2341 ttgcctgcaa aaattcaaaa tatgcaggaa tcgcatttct ttgaagttct gctgcgacgc
     2401 acttatgatt ttttcagcaa caacagaaaa gacgcggcga caaaatattt ctctgttgcc
     2461 gttcatgagt ttttttatca ggaaacgaaa tttcaattcc cgcctgaaca ggaaagagct
     2521 atcgatgata tatgcagaca gataattcgc gaccctggca aacgcagttc cgtttctgat
     2581 ttagcccaaa caacaggata cagcgttgat catttttcga gactttttag gacaattaaa
     2641 ggcacgtcgc cgaaggaatt tatcgttcgg gcaagaattc aggcggcaaa gggtttttta
     2701 aagaactcga atcattcgat tgcccatata gccgaaatac tgggctataa tgaccaattc
     2761 tttttttcca aacagtttca cagttggacc ggccaatctc ccagacagtt caggttgtta
     2821 tgattttacg gctgtcgcac tgacccgtta aaatattatc gctcaatcag ctttttgtat
     2881 gcaattggcg aaaacttgaa ttttgcctta aaagcccgat agaacgtact cagttcttcg
     2941 taaccgacct caaacgcgat agctgtcgcc ggcatatttg tggtctccag caattctctc
     3001 gcgcgtgaaa gccgcaatcc ttcaaggtat tttctgaaac tcatgcctgt gatttttcgg
     3061 caaagattgg taaaatgccg ttccgacagg ttaatttccg ccgccgcgtc cgaaagtgaa
     3121 tacgactgtg aaaaattgtg ctgaatgtat tcgagaattg ccctgacgcg tttttgggcg
     3181 gaagccccgt tctgcatgtt tacgggagat gtccgcagaa gcgaaattaa tattgcattc
     3241 aggctggaaa ttacggatgc ttcataaccg atgagctgct gagattgttc attaaggatt
     3301 tttcgcagct gtttgcgaat ttcggcggcg gtgaacccgc cgggttttac gggcgtctgc
     3361 cgtaatgcgg cgatgtccgg cgatgtgatg ccgtcagcgg tctggcatga aaaatatata
     3421 acgaacagcg tcattggatt acgtggcctg tcgattatct ggtgattgat tccaggttca
     3481 agcagaaccg ccgaattgtc ggacagttcg attcgtttcg attctgcggc agtgttgata
     3541 tccagaaatc ccttacccgc gacaatatag aaaaggctgt aaaacgggtg ggcatgcaag
     3601 gctgtacgga aatctctggt atgcgaatgg ctggcggcaa gtacgccgta acagggaagg
     3661 agcggatttt catctgcatg ggccataagt cataaaatat gataaattgc cggaaatgca
     3721 aatcaattat atcccattat aataagaaaa aatcaacaga tagtcataaa attaatatta
     3781 tgagcgacaa acatgaacaa tatggtaagt ttccggcaag ccttgacgag ctgacaaaac
     3841 tggcagccga tattgatgtc gtcctgccgg taagtcgaca cattgagctg cttgccgagg
     3901 gcattacggt tgacggattc aaattgccga atcgtctggc gattcatccg atggaaggtt
     3961 gcgacggtga atttgacggc cgtcccggcg agttgacgtt acgacgatat caccggtttg
     4021 ccggcggagg cgccggtctg atatggggag aggctatcgc ggttgtgccg gaaggcagag
     4081 ccaaccctcg ccagctccac at
//
LOCUS       DAONFI010000207         4079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_26479_length_4079_cov_3.612823, whole genome shotgun sequence.
ACCESSION   DAONFI010000207 DAONFI010000000
VERSION     DAONFI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26479_length_4079_cov_3.612823"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLK08_07165"
     CDS             complement(<1..615)
                     /locus_tag="PLK08_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPS53314.1"
                     /translation="MSRKCYILAGGGTGGHLYPGLAVAAEIVRNEPDSLVVFACSNRP
                     IDARILTGTPYSFFAQPVMPIRKNPLQWPAVVNAWLKSLLLARKVLADLKPAAVLGLG
                     GFAAGAICKVAAVMGIPTAMINPDAVPGKANRYLAAKVDRIFTQFAETQRYFPKKLQE
                     RIIAAGCPVRAELAGGSRDGAIKFFGLDPDKKTLLVFGGSALAET"
     gene            complement(612..1793)
                     /locus_tag="PLK08_07170"
     CDS             complement(612..1793)
                     /locus_tag="PLK08_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPS53315.1"
                     /translation="MENNEEKPVISLLPLRNNPTGEWLMLCAIGLLGIGTVMVCSAMA
                     SLKTPTMEWYQRVDGKQIIFAGASVLLLFFAWRFNYRWLAAGERVPTLALILLIISLV
                     ACGLVFVPGLGHSVGGKYRWVKITPNFQFQPSELLKFASIIFISALLTRPGAKPKNFF
                     TVLLCGIITILCVGAVIKEDFGTGVLIGVSCAIAMFLAGVPWYYFFVAIPLAGYGGYK
                     LMLSEPYRIARWNAFLNPWDMTNPAAYHPQQSLFAILGGGYTGVGLGNGIRKMGFLPE
                     DSTDFIFAIFSEECGLFGAILLLALLLVLLMMAYKTASRAGDNFGRVLSASLGSLIAL
                     QATLHIAVNLALLPPTGIALPFISAGGTGLLIMSMGVSLIISVSARPKDEILQQYVIK
                     A"
     gene            complement(1793..2920)
                     /gene="mraY"
                     /locus_tag="PLK08_07175"
     CDS             complement(1793..2920)
                     /gene="mraY"
                     /locus_tag="PLK08_07175"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPS53316.1"
                     /translation="MFYWLFSDYFQSAGLGLRIAGAGVVSFLIVMAFGPRFIMFLRRQ
                     KIGDNPEFDHIELNRITSHKSETPTMGGALIVIAMILGILLFANLNNMYVKSGLVAVV
                     WLLALGGVDDWLKLKAAADKTGRDGLKSWEKFVFQVAIAVLLSVFVYRYGQYSFVGES
                     EVYNPAHSFFMPVINRAIPLSFLTYTIIITLVMTGSSNAVNITDGMDGLAAGCLFITG
                     MVFLIISWIVGVASWAEIFNLPFVPFSAEMTVVCSSMLGALLAFMWYNSYPAAVFMGD
                     TGSLPLGGLIGYIAVITRQELVLLVAGGVFVMEILSVIIQVGVFKMTRRSTGVGRRVF
                     KCAPIHHHFHLCGWPETKVVSRMWILAIIFALISLAMLKLG"
     gene            complement(2922..>4079)
                     /gene="murF"
                     /locus_tag="PLK08_07180"
     CDS             complement(2922..>4079)
                     /gene="murF"
                     /locus_tag="PLK08_07180"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPS53317.1"
                     /translation="HSYAQELLNSFKAGVIAVQNSREALLDLGGFYRSVLAAVVIGVT
                     GSNGKTTVKRMIEHILKKKFNGTCSPKSFNNEIGVPLTILGAGAGDDFLICEMGTSAP
                     GEIARLTKAVKPNIAVITSIYPAHLQRLESLDRIAMEKAALLGYLKPQDIAVITSDSP
                     ELDHAVRPYNCRFIRFGTSESAHLRLTDYQSDSHHQRFQINQRDWVELPLPGRHNALN
                     AIAAIAVSARMGFTQQEAAEALADFTGVEMRLQDEKIGPLRVINDAYNANPGSMIAAA
                     AVLAECDGRKVFIAGDMKELGPQSEDLHMQIGREIGLRRPGLVIGVGELGTLLAKGAA
                     KMGTETHCFPSVEEAVKEIPALLCDGDTILIKGSRAMGMERLIDSIRKKFE"
BASE COUNT          891 a         1127 c         1135 g          926 t
ORIGIN      
        1 tgtctccgcc agcgccgagc cgccgaagac gagcagcgtt tttttgtcgg ggtcaaggcc
       61 gaaaaatttt atcgccccat cgcgagagcc gccggccagt tccgcgcgta ccggacagcc
      121 ggctgcgatt atgcgctcct gcaatttttt ggggaaatat cgttgcgtct ctgcgaactg
      181 ggtaaaaatt ctgtccactt ttgcggcgag atacctgtta gccttgccgg gcacggcgtc
      241 gggatttatc atagccgtcg gaattcccat tacggctgcg actttgcata ttgcgccggc
      301 tgcgaacccg ccgagtccga gaactgcggc tggcttgagg tcagccagta cttttcgtgc
      361 cagcagtaga gattttaacc acgcgttgac gacagccggc cactggagcg gatttttgcg
      421 tatcggcatg acgggctgtg cgaaaaagct gtagggcgtg ccggttaata ttctcgcgtc
      481 gattggcctg ttggaacagg caaaaaccac gagcgagtcc ggttcgttgc ggactatctc
      541 agcggctacc gccaggccgg ggtaaagatg gcccccggtt ccgccgccgg cgagaatgta
      601 acattttctg ctcatgcctt tatcacgtat tgctggagga tttcgtcttt aggccgcgcc
      661 gaaacggata tgatgagcga cacgcccatc gacatgataa gcaggccggt tccgccggct
      721 gatatgaacg gcagggcaat gcccgtcggc ggcagcaacg ccaggttgac cgctatgtgt
      781 agcgtagcct gcagggctat caacgaaccc agcgaggccg aaagtactct gccgaaatta
      841 tcgcccgcgc ggcttgcggt tttgtacgcc atcatcagca acaccagcag cagtgccagc
      901 agaagtatcg caccgaacag gccgcattcc tcgctgaata tcgcaaaaat gaagtcggtg
      961 gaatcttccg gcagaaaacc catcttgcgt atgccgttgc caagccccac acccgtgtaa
     1021 ccgccgccga gtatcgcgaa aagcgactgc tgaggatggt acgccgcagg attcgtcatg
     1081 tcccagggat tcagaaatgc gttccatcgc gcaatgcggt acggttcact gagcatcagt
     1141 ttatatccgc cgtagccggc gagtggtatc gccacaaaaa aatagtacca cgggacgccg
     1201 gccaggaaca tggctatcgc gcatgacacg ccgatgagta cgcccgtgcc gaagtcctct
     1261 tttatcacag cgccgacgca gaggatggtt ataattccgc agagcaggac cgtgaagaaa
     1321 tttttcggtt ttgcgcctgg ccgggtgagc agggcggaga tgaatattat cgatgcgaat
     1381 ttaagcagtt cggacggctg gaactggaag tttggcgtta tttttaccca gcggtatttg
     1441 ccgccgaccg aatggccaag gcccggcacg aaaaccagcc cgcacgcgac cagcgatatt
     1501 ataagcagaa taagcgccag tgttggcaca cgctcgccag ccgccagcca ccggtagttg
     1561 aatcgccatg cgaaaaaaag cagcagtacc gacgcgccgg cgaaaattat ctgtttgccg
     1621 tcgactcgct gataccattc catcgtcggt gttttcagcg acgccatcgc gctgcacacc
     1681 atcactgtgc cgatgcccag aaggcctatg gcgcatagca tcagccattc gcccgtcgga
     1741 ttgttccgca acggcagtaa cgagattaca ggtttttctt cattgttttc catcagccca
     1801 gcttcaacat tgcaagagat ataagtgcga aaattatggc tagtatccac attctcgaaa
     1861 cgactttcgt ttccggccag ccgcagaggt gaaagtgatg atgtatcgga gcgcatttga
     1921 agacgcgcct gcccacgccc gtactgcggc gggtcatctt gaaaacgccg acctgtatta
     1981 tgacgctgag tatttccatc acgaatacgc cgcctgccac cagcagcacg agttcctgcc
     2041 gcgttatgac cgcgatgtaa cctatcagtc cgcccagcgg cagtgagccg gtgtcgccca
     2101 taaaaaccgc cgccggatac gaattgtacc acataaacgc cagcagcgcg ccgagcatgg
     2161 atgaacagac caccgtcatt tcggcggaaa agggcacgaa cggcagattg aaaatttccg
     2221 cccagcttgc gacgccaact atccatgaga taatcagaaa gaccataccg gtgatgaaca
     2281 ggcagccggc ggctaagccg tccatgccgt cggttatgtt caccgcgtta ctgctgccgg
     2341 tcatgaccag cgttatgatt atcgtatacg tcaggaacga cagcggaata gccctgttta
     2401 ttaccggcat gaaaaaactg tgagccggat tgtaaacttc cgattcgccc acgaagctgt
     2461 attggccgta gcggtagacg aagacactca gcagtacggc gatggcgacc tgaaagacga
     2521 atttttccca gcttttgagg ccgtctcggc cggttttgtc ggcggcggct ttgagtttca
     2581 gccagtcatc cacgccgccc agcgcgagca gccagaccac tgcaacaagg ccagacttca
     2641 cgtacatgtt gttcaggttc gcgaaaagga gtatccccag tatcatggct atcacgataa
     2701 gcgcgccgcc catggtgggg gtttcagact tgtggctggt tatgcggttg agctcgatgt
     2761 ggtcgaattc cggattatcg ccgattttct ggcgtcgcag gaacatgatg aatcgcgggc
     2821 cgaaggccat gacgattaaa aagctcacca cacccgcgcc tgctattcgc aagccaagtc
     2881 cggcagactg gaaataatcg ctgaatagcc agtaaaacat attattcgaa cttcttcctt
     2941 atggagtcaa tcagccgctc cattcccatg gcgcgtgagc cttttatcaa aattgtgtcg
     3001 ccgtcgcaca acaacgccgg aatttctttc acagcctctt cgacggacgg gaaacagtgc
     3061 gtctccgtac ccatcttggc tgcgcccttc gccagcagtg tgccgagttc gcccactccg
     3121 attaccaagc ctggccggcg aagcccgatt tcacggccaa tttgcatgtg caaatcttca
     3181 ctttgagggc cgagctcctt catgtcgccg gcgatgaaga cttttctgcc atcgcattcc
     3241 gcaagcactg cggcggcggc aatcatgctg ccggggtttg cgttgtatgc gtcgtttatc
     3301 acccgcagag ggccgatttt ctcatcttgc aggcgcattt ctacgccggt gaagtccgcc
     3361 agcgcttcgg cggcttcctg ctgcgtgaaa cccattctcg cacttacggc gatggccgct
     3421 atcgcgttga gggcgttatg tctgccgggc agcggaagtt ccacccagtc acgctggttt
     3481 atctggaaac gctgatgatg gctgtcggac tggtagtcgg tcagccggag atgtgcggat
     3541 tcggatgtgc cgaagcggat gaatctgcaa ttatacggcc gtactgcgtg gtcaagttcg
     3601 gggctgtcgg atgtgatgac ggcgatgtct tgtggtttga gatagccgag cagggctgct
     3661 ttttccatgg cgattctgtc gagcgactcc agccgttgaa gatgggctgg ataaattgaa
     3721 gtaatgacgg cgatgtttgg tttgactgct ttggtcagcc gcgctatttc acctggtgcg
     3781 ctggtgccca tttcacatat caggaaatcg tcacctgcgc ctgcgccgag tatcgttagc
     3841 ggcacgccga tttcgttatt aaaacttttt ggactgcacg tgccgttgaa tttctttttg
     3901 aggatgtgtt caatcatccg tttgacggtg gtttttccgt tcgagccggt tacgcctatg
     3961 actacggcag ccagtacgct gcggtagaat ccgccgaggt cgaggagggc ttccctggag
     4021 ttctgtactg caattacgcc ggctttgaag gagttcagca gttcctgcgc atacgaatg
//
LOCUS       DAONFI010000208         4077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_26506_length_4077_cov_3.422675, whole genome shotgun sequence.
ACCESSION   DAONFI010000208 DAONFI010000000
VERSION     DAONFI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26506_length_4077_cov_3.422675"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLK08_07185"
     CDS             <1..267
                     /locus_tag="PLK08_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53318.1"
                     /translation="SQPTPQTATSAKSSLICSNTNLHYNGHELFTMTFKKNLWLKSSI
                     VVAADKIKIRTHCNSIRFAFKQDAQKKFETEIVHMHNLTARNCQ"
     gene            344..416
                     /locus_tag="PLK08_07190"
     tRNA            344..416
                     /locus_tag="PLK08_07190"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:378..380,aa:Glu,seq:ctc)
     gene            complement(455..1015)
                     /locus_tag="PLK08_07195"
     CDS             complement(455..1015)
                     /locus_tag="PLK08_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPS53319.1"
                     /translation="MTVLYAAVLIVAFIVIAWFIGTYNSFVRLKQQCRESWSGIETEL
                     KRRYNLIPNLVETVKGYAAHERQTLEMVIEARNRAAANNGTPAQQAKDENLFINALGN
                     LFALAENYPQLKASENFLSLQKELTNTEDRIQAARRFYNANVRDINTRIAVFPSNMIA
                     GLFSFQPEEFFEVTDSSMRQTVTVKM"
     gene            complement(1037..1723)
                     /locus_tag="PLK08_07200"
     CDS             complement(1037..1723)
                     /locus_tag="PLK08_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53320.1"
                     /translation="MASSDLSAFIPLLFIAIFIAAVVLSFMSARKRRKMLAEWATSRN
                     LLFTPEKNSCFEHVHPAFKCFKYGDSRYAFNIITGDWHGWDMTAFDYHYTTGSGKNRQ
                     THLFSCIILKSPFPLKPLSIRPEGFFDKLTEFFGLDDIDFESAEFSRRFFVKAPEKKW
                     AYDILHSRAMELLLNRQAAKYYIEFATDEVLIRRSDSTWQPADIEQAVDTIIALLDMI
                     PDYVKKQMQS"
     gene            complement(1902..2837)
                     /locus_tag="PLK08_07205"
     CDS             complement(1902..2837)
                     /locus_tag="PLK08_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPS53321.1"
                     /translation="MKFFMYSMLVAFFILGGCEMVVPEKSRYAAPEETDGKVVFIPTF
                     DDGPIDQKFVEEGVYPKNQEEMLASLHKTLDTLKKHNTTAVFYAVYVRPEEDAPKWSP
                     KSSEKEMRAAFLSGIKAIADAGHVVAIHACNHDEYHNLFMSAKDAQDDIKRLIFELDR
                     TGVAYYPTWRVPYGGMRTLMDEQLVAKNLSLPVETWLVDSHDWTTNHDSTDFLKAWYK
                     DDAKWTANVKKNLRQDMSVSKFRKERQRDILFHVSDRTSTFLDEFLNYVDAEGRKIYG
                     KKFTFLPIDSPYKGVMLEHYLNNKPAQGKAIPPQL"
     gene            complement(2884..3885)
                     /locus_tag="PLK08_07210"
     CDS             complement(2884..3885)
                     /locus_tag="PLK08_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53322.1"
                     /translation="MRFLSAIFGVVFLTFCAGGCGCSLDRDTDPHCRMIGTPADWNSP
                     YLPYKAVTSIAVSQNGKLLAAARMEYGVTVYSLPWGFEECQIPARKGGEIMQVEFADN
                     DTLLTAGKDKQLRFWNPHDGKLIKSYQHEGLWFMPFALSPNRKFIATLESDDKVYVWD
                     FATGGLIKTMDGQGGGVQSVTFLPDNNRLVTSSNYTNEVILWDIAQGRKLWTTPAETF
                     SLAVSPDGKLMAFRKDHSIEIWNLDSRKKLYTLMPQQFGIGHFSFAFSPDGKCLGAAE
                     LGGDIVMWDLKTQRMLCDYNGPVGDGLCIEPGPESRYFYVGGYRNSTVCRWPIPDEE"
     gene            complement(3882..>4077)
                     /locus_tag="PLK08_07215"
     CDS             complement(3882..>4077)
                     /locus_tag="PLK08_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53323.1"
                     /translation="ELEIVRQDSFAWKPTGQKAKPVKLLADSLQDKDADYIFIGHSQG
                     ANIIMKMLQIGCDDSGEKTK"
BASE COUNT          917 a          951 c         1038 g         1171 t
ORIGIN      
        1 agccagccaa cgccgcaaac agcaacaagc gcaaagtcaa gtttaatatg ctctaataca
       61 aatcttcatt acaatgggca tgaactcttt acgatgactt tcaaaaaaaa tctttggctg
      121 aaaagttcga tagttgttgc cgctgacaaa attaaaatcc gaacccattg caattcaatt
      181 cggtttgcat tcaagcagga tgctcaaaaa aaattcgaaa cggaaatagt tcatatgcat
      241 aatctcacag cacggaattg tcagtaagga aatatctgtg ctgatttttg ggcttaagcc
      301 ttgattttaa tgtctcattt gtgcagaata tgcaccttgt ttcggcccca tcgtctagtg
      361 gcccaggaca cagggttctc atccctgcaa ccggagttcg agtctccgtg gggtcagttt
      421 cagccttctg tggcggaagg ctgaaaggcc atcgctacat ttttaccgta acagtctgcc
      481 gcatggaaga gtcggttact tcgaaaaatt cttccggctg gaagctgaaa aggccggcaa
      541 tcatgtttga cgggaaaacc gctattcggg tattgatgtc tctgacgttg gcgttgtaga
      601 accttctcgc cgcctgtatt ctgtcctctg tattggtaag ttctttctgc aatgaaagga
      661 aattttccga cgccttcaat tgcggataat tctccgccag cgcgaaaaga ttgcccaatg
      721 cgtttatgaa cagattttcg tcctttgcct gctgagccgg cgttccattg ttggctgctg
      781 cccgattacg agcttcgatt accatttcca acgtctgccg ttcatgtgcg gcgtagcctt
      841 ttactgtctc gacgaggttt ggtatcagat tgtaacggcg cttgagttcg gtttctatgc
      901 cgctccagct ttccctgcat tgctgtttga gccggacgaa agagttatat gtgccgataa
      961 accacgcaat tacaataaac gccactatta aaactgccgc gtaaagtact gtcattgtca
     1021 gctccgtgag tattttttat gattgcatct gctttttaac gtaatcgggt atcatgtcca
     1081 gcagggcgat tatggtatcg acagcctgtt cgatatcagc cggctgccac gttgagtctg
     1141 agcggcggat aagaacttca tcagttgcga attcaatata atatttggca gcctggcggt
     1201 tgagcagaag ttccatggcg cggctgtgca gaatgtcata agcccatttc ttttccggtg
     1261 cttttacgaa aaaacggcgg ctgaattctg ccgattcgaa gtctatatcg tccaagccga
     1321 agaattccgt caatttgtca aaaaaacctt ccggccgaat tgatagcggc tttagcggga
     1381 acggggattt tagtattatg catgagaaca gatgcgtctg gcggtttttg cctgagccgg
     1441 tagtgtagtg atagtcaaat gcagtcatgt cccagccgtg ccagtcgccg gttatgatgt
     1501 tgaacgcata gcgtgaatca ccgtatttga agcatttgaa tgccggatgg acgtgttcaa
     1561 aacagctgtt cttttcgggc gtgaatagca ggtttctgga tgtagcccat tcggcgagca
     1621 ttttgcgacg ttttctggcg gacatgaacg acagaactac agccgctatg aatatcgcta
     1681 tgaacagtag tggtatgaat gccgataaat cactgctggc caatttgttc tcctgttgtt
     1741 gagaaaaaat cctgaattaa caaaatttgc cgtaagtata ggtttcaggt gctgttaact
     1801 aactttctac ttgtaaataa tttacgatac ttaaaagttg aaaattgttt gtcgtttagt
     1861 tcttgaattc tgttattttt tcctgtagcg ctgttgcgat attaaagttg cgggggaatg
     1921 gcttttccct gagcgggttt attgttgagg taatgttcaa gcatgactcc cttgtatggt
     1981 gaatcgatgg gcagaaacgt gaattttttg ccgtagattt tcctgccttc ggcgtcgaca
     2041 tagttcagga attcgtcaag aaacgtgctc gttctgtccg agacgtggaa gagtatgtcc
     2101 cgctgccgtt ctttgcggaa ttttgagacg gacatgtcct gccggagatt ttttttgacg
     2161 ttagccgtcc atttggcatc gtccttgtac catgccttta ggaaatccgt cgagtcgtga
     2221 ttcgtcgtcc agtcgtggct gtcgaccagc catgtttcca ccggcaggct gaggtttttt
     2281 gcgacgagct gttcgtccat taaagtgcgc atgccgccgt acggcactcg ccacgttgga
     2341 taatacgcga cgccggtgcg gtcaagttcg aatatcagcc gtttgatgtc atcctgtgcg
     2401 tctttggcgg acatgaacag gttgtgatac tcgtcgtgat tgcaggcgtg gatggctact
     2461 acgtggccgg cgtcggcgat tgccttgatg ccgctgagaa aagctgcccg catttccttt
     2521 tccgaacttt tgggcgacca ttttggcgcg tcttcttcag gccgaacgta gactgcataa
     2581 aaaacggctg tcgtgttgtg tttcttgagc gtgtcgaggg ttttgtgaag gctggcgagc
     2641 atctcctcct gatttttggg ataaacgcct tcttcgacga atttctgatc tattgggccg
     2701 tcgtcgaatg tcggtatgaa caccactttg ccgtcggttt cctcaggggc ggcgtaacgc
     2761 gatttttccg gcacgaccat ttcacagccg ccgagtatga aaaatgcgac taacatgctg
     2821 tacatgaaaa atttcaattt agttctcctt ttatattatt ccgaaggtta tgtttcggcg
     2881 gcgtcattct tcgtccggta tgggccagcg gcataccgtt gaattgcggt aaccgcctac
     2941 gtaaaaatac ctgctctcag ggcctggttc tatacaaagg ccgtcgccta cggggccgtt
     3001 ataatcgcac agcatccttt gggtttttaa gtcccacatc actatatcgc cgccgagttc
     3061 agctgcgccg agacatttcc cgtccggcga aaaggcgaaa gaaaaatgac ctatgccgaa
     3121 ttgttgtggc attagggtgt agagtttttt gcggctgtcc agattccata tttcgatgga
     3181 atggtctttc cggaaagcca tgagtttgcc gtcgggcgag actgcgaggc tgaaagtctc
     3241 agccggcgtc gtccagagtt ttcggccttg tgctatgtcc cacaaaataa cttcgttcgt
     3301 atagttgctc gacgtcacaa gacggttgtt gtccggcaga aatgtcactg actgaacgcc
     3361 gccgccctgg ccgtccatgg tcttgattag tccgccggtg gcaaagtccc atacatacac
     3421 tttatcgtcc gattcaaggg ttgcgataaa tttcctgttc ggcgagaggg cgaagggcat
     3481 gaaccacaag ccctcatgct ggtagctttt tatcagcttg ccgtcgtgcg gattccagaa
     3541 tcgcagttgt ttatccttgc cggcggtcag aagcgtatcg ttgtcggcga attcaacctg
     3601 cattatttcg ccgcctttgc gagcaggaat ttgacattcc tcgaaccccc acggcagaga
     3661 ataaacggtt acgccgtatt ccattctggc ggcggctaac agtttgccgt tttgggaaac
     3721 ggctatgctg gtgactgctt tatagggcag gtagggcgag ttccagtctg ccggcgtgcc
     3781 aatcatgcgg cagtgcgggt cggtgtccct gtcgaggctg cagccgcagc cgcctgcgca
     3841 aaacgtcaga aaaaccaccc caaaaatagc ggataagaat ctcatttcgt tttttctccc
     3901 gaatcgtcac agccgatttg aagcattttc atgatgatgt tggcgccttg cgagtggccg
     3961 ataaagatat aatcggcatc cttgtcctgc agggagtcgg cgagcagttt gacgggcttg
     4021 gctttctggc cggtcggttt ccatgcaaaa ctgtcctgcc ggactatttc gagttcc
//
LOCUS       DAONFI010000209         4066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_26602_length_4066_cov_4.373722, whole genome shotgun sequence.
ACCESSION   DAONFI010000209 DAONFI010000000
VERSION     DAONFI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26602_length_4066_cov_4.373722"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="PLK08_07220"
     CDS             complement(<1..2323)
                     /locus_tag="PLK08_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPS53324.1"
                     /translation="MLPAVAICAGIVISWRVELGQRLWFVGCVLGAVLAVFFSFQHHR
                     RLMAHICLGLCLFCLGGGRFDQSYRSIGKDDIVTYTAETAIPATIKGCVSSYPLLVDP
                     PKELGYRSLPRVKFLLDVEAVKTASGFVPATGICRVTVREPYSFPKPGERVELVGLLG
                     RYDKADNPGQFDSLSQARLTGTFTWFTLPTSSGITVLADTPTGPISRFWWRLKSSVRQ
                     RLTETGDMQGGMLLSALLIGERHPALQDLNRIMMRAGVAHFMSISGLHLGIFLGFIYL
                     LCRLFMIGPRGAAVVVLAALAWYLCFAEARAPLLRSAIMAACLAGGLAIGRHYSPLNA
                     LTAALIILLLIDPRQLFTPGFQLSFAIVLGLILFTGSFKRLLFGWWLRRRGLLVFKPN
                     ERFRKWANFQLADAAMNFLSVCVCAYLISVPLAAIHFGFFSPYGMVLGIVLFPIIVAI
                     LIPGYMAMALAIPMPNLAGKFAELAGWFAGLMADLMHAADKLPLLSITVKPVGGMWVL
                     SYYLALFAILFIRGRWWRKAAAGCLLTLFVAMTIVTQLPAAASGSVQLNFLSVGYGQV
                     CVMRLPDGQTWLLDAGSRSQADVFYRIYQPFLRASRLPKPQTAILSHPHPDHYNVLTD
                     VYDNAGFEKLYATSFVFAENAAGIPSLAQMITALRNNCLETKRPLCMGQTLEPQKDVK
                     IEVLWPDVAEAKDWPTNDTSLVLKITCGGKSALLPGDVGEKAQKSLLAKQRRKLRADI
                     LVLPHHGGWNAALPEFVAAVNPTVAPAANLTLGS"
     gene            complement(2631..3041)
                     /gene="metG"
                     /locus_tag="PLK08_07225"
     CDS             complement(2631..3041)
                     /gene="metG"
                     /locus_tag="PLK08_07225"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HPS53325.1"
                     /translation="MCECSNCSNEPTAAAAPQETQHQPQKPEITFDDFAKLDLRVGRV
                     VEVAAHPNADKLLVLKVDLGFETRTICAGIKAYYAPEALLGRNIIIVANLAPRKLRGV
                     ESRGMLLAASNDDHSQVVLLTTEKEDVAPGSACG"
     gene            complement(3132..3506)
                     /locus_tag="PLK08_07230"
     CDS             complement(3132..3506)
                     /locus_tag="PLK08_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53326.1"
                     /translation="MNLDLAGQICESIMLVAFGLSWPISILKSYRLKFVGGKSMWFLV
                     LIIVGYAAGTVAKFLKAFETGHPLDFSTWLYILNGILVFIDLSLYFRYRHNHEPITKE
                     VAEDIARIIETDMQADADSDNV"
     gene            complement(3537..>4066)
                     /locus_tag="PLK08_07235"
     CDS             complement(3537..>4066)
                     /locus_tag="PLK08_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPS53327.1"
                     /translation="ASLLAEVLPHVDIFVPSLDEIVYMLAPDRMEEGVSVQLLRDISE
                     AIFELGSAVTMIKLGDRGIYAGATSREERLADVSLIQSSDDWCGREIYRPCFMANLVG
                     TTGSGDCTIAGFLGGLTHEMNIEECATMAVAVGAFSVEAADATGGIKPWAKVLERINA
                     GWETHLPAPQLEELM"
BASE COUNT         1012 a         1170 c         1018 g          866 t
ORIGIN      
        1 cgctgcccag cgtgaggttc gcggcgggcg ccactgtggg attgacggcg gcgacaaact
       61 ccggcagagc cgcattccag ccgccgtggt gcggcagaac cagtatgtca gccctgagct
      121 tccgacgctg tttggcgagc agcgattttt gggctttttc gcccacatca cccggcagca
      181 gagcggattt cccgccacaa gttattttaa gtacaagcga agtatcgttc gtcggccaat
      241 ctttcgcttc ggcgacgtcg ggccatagca cttcgatttt cacatccttt tgcggctcaa
      301 gcgtctggcc catgcaaaga ggccgcttcg tttccaaaca attattgcgg agggcggtaa
      361 tcatttgtgc caggcttgga attccggcgg cattttcggc aaagacaaac gacgtggcgt
      421 ataatttctc aaaacctgcg ttgtcgtaaa cgtccgtcag tacgttgtaa tggtcgggat
      481 gcgggtgcga aagtatggcc gtttgcggct ttggcagccg cgaagcccgc aaaaacggct
      541 gatatattct gtaaaaaacg tcagcctgcg agcgcgaacc ggcgtcaagc agccaggttt
      601 ggccgtccgg cagacgcatt acgcaaacct gcccgtaccc gacagacaga aaattcagtt
      661 gaacgctgcc ggatgccgca gccggcagtt gcgtaactat cgtcatggca acaaataacg
      721 tcaaaagaca gccggctgcc gctttccgcc accatcgtcc gcgaatgaac agtatcgcaa
      781 acagcgccag ataatatgac agcacccaca ttccgccgac cggctttacg gttatggaca
      841 gtagcggaag tttgtcggct gcatgcatga ggtcagccat aagtccggcg aaccagccgg
      901 cgagttcggc aaatttcccc gccaggttcg gcatcggaat ggctaacgcc atagccatat
      961 atccaggaat cagaatcgcc acgattatcg ggaacagtac gatacccagc accattccat
     1021 aggggctgaa aaaaccgaaa tgaatcgcag ccagcggcac ggatatcaga tacgcacaga
     1081 cgcagacgga aagaaaattc atcgccgcgt cggcaagctg gaaattggcc cattttctga
     1141 atcgctcgtt aggcttaaac accagcaatc ctcgccgccg cagccaccag ccgaacagta
     1201 atcgtttgaa actgccggta aaaagtatca gccccagaac tatggcgaag ctgagctgaa
     1261 aacccggcgt gaacagctgc cgcgggtcta tcagaagcag gattatcagg gctgcggtca
     1321 atgcgttcag cggcgagtag tgtcgcccga tggctaagcc gccggcgagg caggctgcca
     1381 ttatcgcact tcgcaacagc ggcgcacggg cttccgcaaa gcacagatac cacgccagcg
     1441 ccgccagcac caccacggct gcgcctcgcg ggcctatcat gaacagccgg cagagcagat
     1501 aaatgaatcc gagaaaaatc cccagatgaa ggccggagat gctcataaag tgggcaacgc
     1561 cggctcgcat catgatgcga ttcaaatcct gaagcgcagg atgacgctcg ccgatgagca
     1621 gcgccgaaag cagcatgccg ccctgcatgt cgcctgtctc cgtcagccgc tgacgaaccg
     1681 acgatttcag ccgccaccaa aaccggctta tcggccctgt cggcgtatca gccagcaccg
     1741 taatcccgct cgacgtaggc aacgtaaacc acgtaaacgt ccccgtcagc cgagcctgcg
     1801 acaatgaatc aaactggccg ggattatccg ccttatcata ccggcccaat aatcccacaa
     1861 gctccacgcg ttcgcctggt ttggggaaag aatagggctc acgaacggtt acgcgacata
     1921 tgcccgtcgc cggaacgaaa ccactcgcag tttttacagc ctccacatcg agcagaaatt
     1981 tgaccctcgg cagcgagcgg taacccaact ctttcggcgg gtcgaccagc agcggataac
     2041 tgcttacgca gcctttgata gtggccggaa tcgcagtttc ggcggtatat gtgacaatat
     2101 cgtccttgcc gatactgcgg tacgactggt cgaatcgtcc gccgccaagg cagaacaggc
     2161 agaggcccag acagatatgg gccatcaatc ggcgatgatg ctggaaagaa aaaaagacgg
     2221 ctaaaacagc cccaagaacg caaccgacaa accacagccg ctggccaagc tcaacccgcc
     2281 atgaaatcac aatgcccgca catatcgcaa ccgcaggcag caacagcggc agtctctcaa
     2341 gcctgccatt attaaaaatt ttttgtaaaa gaacagattt ttcaaagccg ttcgcgataa
     2401 tctctccccg ctaaaaaagt aacaaaattc aataaacacg cggcaagtaa atctttcaaa
     2461 aacttccagg tatcgtatat aggttatcca taaaaaatgt gcttttttta caaaaccaat
     2521 ttcaaaaaca ttagcgttgc cttccacgtc tcttgtctta tgaaactttt atccgcctta
     2581 cgccggctgt cgtcttttac ctgttcattt taaaaacata gcgactgttt tcatccgcag
     2641 gcgctgcctg gcgcgacgtc ttctttctcc gtcgtcagga gcacgacctg cgaatggtcg
     2701 tcattgctgg cggcaagaag catgccacgg ctctcgacgc cgcggagctt tcgcggtgca
     2761 aggttagcca cgatgatgat attgcggccc agcagtgctt ccggtgcata atacgccttt
     2821 atgccggcac atatggttcg agtctcaaaa cccaggtcaa ccttcaacac aagcagtttg
     2881 tctgcattgg gatgggcagc cacttcaacc actctgccga cacgcaaatc gagctttgcg
     2941 aaatcgtcga aggtgatttc aggtttttgg ggctgatgtt gcgtttcctg cggcgccgcg
     3001 gcggcagttg gctcattact gcaattcgaa cattcgcaca ttttcaaaaa tcctttccgt
     3061 ttttataaaa aatttcgtac gtatcagaaa ttctcaagca cgacgctgcc ggcggcaaat
     3121 atcgcagttt tttatacatt atcactgtcg gcatccgcct gcatatcggt ctcgataatt
     3181 ctggcgatat cttcagcaac ttctttggtt atcggctcat ggttatggcg atacctgaaa
     3241 taaaggctca ggtcgataaa aaccagtatg ccgttaagga tgtacagcca tgtgctgaaa
     3301 tcgagcggat ggccggtttc gaaggctttg agaaacttcg caaccgtgcc ggcggcatag
     3361 ccgacgatta tgagcacgag aaaccacatg ctcttgccgc cgacgaattt aaggcgatag
     3421 cttttaagta tcgatatcgg ccatgacagg ccgaacgcca caagcattat cgattcgcat
     3481 atctggcccg ccaggtcaag attcattttc gacactccct gcttgctgca gccgttttac
     3541 atcagttctt caagctgcgg ggcaggcaga tgcgtttccc agccggcgtt tatccgctcc
     3601 aggactttcg cccagggctt tatgccgcct gtggcgtcgg cggcttccac gctgaacgct
     3661 cctacagcca cagccatcgt ggcacattcc tctatattca tctcatgcgt caaaccgccg
     3721 agaaatccag caatcgtgca atcgcccgaa ccagtagtgc cgacgagatt ggccataaag
     3781 cacggccgat atatttcccg gccacaccag tcgtcggaag attgtatcag cgatacgtcc
     3841 gccagccgct cttcgcggct ggtcgcgccc gcatatatgc ccctatcgcc gagcttaatc
     3901 atggttacgg ctgagccgag ttcaaagata gcctctgaaa tgtctctcag caactgaacc
     3961 gaaacgcctt cttccatcct gtccggcgcg agcatgtaaa ctatttcatc aaggctgggc
     4021 acgaaaatat cgacgtgcgg cagtacttca gccagcagcg aggccc
//
LOCUS       DAONFI010000210         4064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_26620_length_4064_cov_4.050137, whole genome shotgun sequence.
ACCESSION   DAONFI010000210 DAONFI010000000
VERSION     DAONFI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26620_length_4064_cov_4.050137"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLK08_07240"
     CDS             complement(<1..712)
                     /locus_tag="PLK08_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HPS53328.1"
                     /translation="MAKVSSASAEHIIGRLERFKVEIPSWGFSQTGTRFGKFLQDAAA
                     VDMDDKLADAGQVNKYTGMTPTVAVHVLWDFPNGYDRQVVKIAEKYGVKIGAINPNVF
                     QDQCYKYGSITNRDAKIRKRATGHIIDSIEIGKKTGSEYVSLWFADGANYPGQADIRQ
                     RKAWATKSLQDAHKTLGRNQIMLLEYKPFEPATYFTDFCDWGASYIFAKNAGKNAKVL
                     VDLGHHLLDGERVVERHDR"
     gene            1048..2613
                     /locus_tag="PLK08_07245"
     CDS             1048..2613
                     /locus_tag="PLK08_07245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPS53329.1"
                     /translation="MFSLTEYIDKKTLSQLQLAFGAVAGYPIAICSADGTAVFVNNRI
                     TPSHELFAQTSIKYEGEIIGYLRMYQPADTDKLDVALESFRIKLLKLMANVLIRFCRE
                     TTLRRARIRQIMTINRVMANITSGGNLQETLNLVTQKIVEALNAKACAIRLLSPDGQN
                     LIIRALHCINPQYLDKAPMPVSESPLDQQTLATTKTVYVEDMTTDPRVKDPEVSAREG
                     LISALCVALIYKDKPIGVLRVYKDYKYEFDWFERQMLKTMAGAAAAVIADAQLFKEVK
                     ESMRIKRQLSMARVVQRRLIPQKMPQAAGYDISPFYVPCQELSGDFYDFLELPGKNIG
                     IVICDVVGKGVRASLLMATIRASLRAHASNVYEITDVLEKVNRDLCAGTEVSDFATIF
                     YGVLDTKINRLTYCCAGHMPPVVVRNGKIFHLEARGGVIGIMPDMEYPKEFFDLKSGD
                     VFFTYTDGLSEAANYHGEEFGRHRVEKALLGAVNQNLSAEGIDKYCVWEMRKFTGIQY
                     NHDDLTMLAIKVR"
     gene            2803..3834
                     /locus_tag="PLK08_07250"
     CDS             2803..3834
                     /locus_tag="PLK08_07250"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPS53330.1"
                     /translation="MKYIKQNISDMQAYVPGEQPRELHSVIKLNTNENPYPPSPRITE
                     FLRSFDAERLRNYPDPAGREFCETAAELFDVPPAWVQIGNGSDNLLVMLARACRGPVV
                     VPTPTFPFYETQAIIENDGLKTVPFDEDFNLPVQNLIRAGGAVTYVANPASPTGGAVS
                     VEVLAEMAASLHNSGLLVIDEAYADFAGFTAVPLVKEYDNVLVIRTLSKGYSLAGLRL
                     GFAVSQPEILRELWKTKEIYNVGVLPLEIGRIALQDQEWKNQNVQKILNSRKDVSAEL
                     KARSWRVYPSMANFVFAAPADGNSAAEYENLKEKGIFVRYFEKPGMLRITMGRPEENW
                     TMLESLRQI"
     gene            3831..>4064
                     /locus_tag="PLK08_07255"
     CDS             3831..>4064
                     /locus_tag="PLK08_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HPS53331.1"
                     /translation="MNRTAEIKRKTNETEIEISLNLDGKGCTDCKTGVGFLDHMLDHL
                     GKHSLCDMTVRAKGDLQVDAHHTVEDVAICLGEA"
BASE COUNT         1090 a         1059 c         1021 g          894 t
ORIGIN      
        1 cccggtcatg ccgctcgacg acacgctcgc cgtccagcag gtgatggccg aggtcgacga
       61 ggactttggc gtttttgccg gcatttttgg caaaaatata gctggccccc caatcgcaga
      121 aatcagtaaa atacgtcgcc ggctcaaaag gtttgtattc cagcagcatt atctgatttc
      181 tgccgagcgt tttgtgggca tcctgcagcg acttggtagc ccatgcctta cgctggcgga
      241 tatcggcctg ccccggatag ttcgcaccgt cggcaaacca cagcgaaacg tattcactgc
      301 cggtcttctt gccaatctca atagaatcga tgatgtggcc ggtagcacgc ttgcggattt
      361 tagcatcacg attggttatc gaaccgtact tgtagcactg gtcctgaaac acattgggat
      421 taatcgcacc aatctttacg ccgtattttt ccgcaatttt aacgacctgc cggtcatagc
      481 cgttcgggaa atcccaaagt acgtgaacag ccaccgtcgg agtcataccg gtatatttgt
      541 taacctggcc ggcgtcggca agtttgtcgt ccatatcgac ggcggcggcg tcctggagaa
      601 atttgccaaa ccgcgtgccg gtctgcgaaa atccccacga aggaatttcc accttaaacc
      661 gttccagcct cccgatgata tgctcagccg acgctgaaga tactttcgcc atggacaatc
      721 tcctgtaata ttcgattaca cagtgatttg cgtcattccg ccttctcaaa agccggacat
      781 gcacaatcca caccggcaat ccctataaaa aaatttattg gtgcagggcg ttcggaatta
      841 taacgctttt ttaacggaat acaaacgcta tttgcatgat ttcatgggaa tatggcagat
      901 gaaaactgga actcaaaaaa tattttttta cagaggaact tgccaaccat gtccgcatcc
      961 ctagaatata gtcaggttca aggacgaaag ataaagttgc tcctacggtg ggccatgctc
     1021 ggcacaaccg tcggacagga aaacggaatg tttagcttaa ctgaatatat cgataaaaaa
     1081 actctcagcc aattacagtt ggcattcggc gcagtagccg gctatccaat agcaatatgc
     1141 agtgctgacg gaacggcggt atttgtcaat aaccgtatca ctccatcaca tgaacttttt
     1201 gcgcagacca gtataaagta tgaaggcgaa ataatcggat atctccgcat gtaccagccg
     1261 gcggatacgg acaaattgga tgtcgcgctg gaatcgtttc gcataaaact gctcaagctt
     1321 atggctaacg ttctcatccg attctgccgc gagaccacgt tacgtcgcgc gcgaatccga
     1381 cagattatga ccataaaccg cgtgatggcc aacatcacca gcggcggcaa tcttcaggaa
     1441 acgctcaatc tcgtcacgca gaaaatcgtg gaggcgctca acgccaaggc ttgcgcgata
     1501 cggctgctca gccctgacgg ccagaatctc ataatacgcg ccctgcattg catcaatccg
     1561 caatacctgg ataaggcacc catgccagtg tccgaaagtc ctctcgacca gcagacactt
     1621 gcaacgacga aaaccgtcta cgtcgaagac atgacaaccg accctcgcgt aaaagacccc
     1681 gaagtctccg ccagggaagg ccttatcagc gcattgtgtg tggctcttat atataaggat
     1741 aaacccatcg gcgtattacg ggtttataaa gactacaaat atgaattcga ctggttcgaa
     1801 cggcagatgc taaaaacgat ggcaggcgca gcggcggcgg tcatcgcgga cgcccagctt
     1861 ttcaaggaag tcaaggaatc catgcgtata aagcggcagc tttccatggc tcgcgtggta
     1921 cagcggcggc tgataccgca gaaaatgccg caggccgcag gttatgacat atcgccattc
     1981 tacgtgccct gccaggaact gtcgggcgac ttttacgatt ttctggaact gccgggaaaa
     2041 aatatcggca tagtgatatg cgacgtcgtg ggcaaaggcg tccgtgcgtc attattgatg
     2101 gcgaccatcc gtgcatcgct gcgagcgcac gcttcgaacg tctacgaaat caccgacgtg
     2161 ctcgaaaagg tcaatcgcga cctgtgtgca ggcacggagg tctcggactt cgccacgatt
     2221 ttttacggcg ttctggatac gaagataaat cgtctgacct attgctgcgc cggccatatg
     2281 ccgccggtag tagtgcgcaa cggcaagata tttcatctcg aagcacgagg cggcgtcatc
     2341 ggcataatgc cggacatgga atacccaaaa gaatttttcg acctcaaaag cggcgacgta
     2401 tttttcacct ataccgacgg cttgagcgag gcggctaact accacggcga agaattcggc
     2461 cgacatcgcg tcgaaaaagc acttctcggc gccgtcaacc agaatctttc cgccgaaggc
     2521 atagacaaat attgcgtatg ggaaatgcgt aaattcaccg gcatacagta caatcacgac
     2581 gacctgacaa tgctggcgat aaaggtcaga taacaaaatt cagttttggg caggcttggg
     2641 gtcgcagcca ggaagaagac tgaagcatat tcggcgatat ttcgaatttt ccccgacgca
     2701 acaaggcgca atttcaaatt taacatgctc taattcccaa tttctgattt tttattttcg
     2761 ttattttatt tataatctta tgtctgcaag gcagaaagca tcatgaaata tatcaagcag
     2821 aacatatcgg acatgcaggc gtacgtccct ggcgaacagc cgcgggaact gcacagcgta
     2881 atcaaattga ataccaatga aaatccctat ccgccgtcgc cgcggataac ggagtttctg
     2941 cgcagtttcg acgcggaacg tttgcgaaat tatcccgacc cagccggcag agaattttgc
     3001 gaaacagcgg cagagctgtt tgatgtgccg ccggcctggg tacaaatcgg caacggcagc
     3061 gacaatctgc tggtcatgct ggcccgtgcc tgccgcgggc ctgtcgtcgt accgacgccc
     3121 acgttcccgt tttacgaaac acaggcaata atagaaaacg acggccttaa aaccgtgccg
     3181 ttcgacgaag atttcaatct gccggtacag aatctgataa gagccggcgg agccgtcaca
     3241 tacgtagcca atcccgccag cccaaccggc ggagccgtca gtgttgaagt tctggctgaa
     3301 atggctgcca gcctgcacaa cagcggcttg ctggtgatag atgaggcgta tgccgacttt
     3361 gcaggtttta ctgctgtgcc gctcgtgaaa gaatacgaca acgtactggt catccgcaca
     3421 ttatcgaagg gttattcgct agctggcctg cggctcggtt tcgccgtcag tcagccggaa
     3481 attcttcgcg aactctggaa aacaaaggaa atctacaacg tcggcgtact tccgctggag
     3541 ataggccgca tcgccctgca agaccaggaa tggaaaaacc agaacgtaca aaaaatcctg
     3601 aactccagaa aggatgtgtc agccgagctt aaagcccgtt catggcgggt ttacccaagt
     3661 atggctaatt tcgtatttgc ggcgccggcg gacggaaact cggctgccga atatgaaaac
     3721 ctcaaggaaa agggcatatt tgttcgatac tttgaaaagc ccggcatgtt gcgcataacc
     3781 atgggccggc cggaagaaaa ctggacaatg ctggaatcat tgaggcagat atgaacagaa
     3841 cagccgaaat aaagcggaaa acgaacgaaa cggaaataga aatctcgctc aacctcgacg
     3901 ggaaaggctg taccgactgc aaaaccggcg tagggttttt ggaccacatg ctcgaccatc
     3961 tcggcaaaca cagcctctgc gatatgactg tccgggcaaa aggcgacttg caggtagacg
     4021 cacatcacac ggtggaagac gtcgcaatat gcctgggtga agcg
//
LOCUS       DAONFI010000211         4039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_26878_length_4039_cov_4.038153, whole genome shotgun sequence.
ACCESSION   DAONFI010000211 DAONFI010000000
VERSION     DAONFI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26878_length_4039_cov_4.038153"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2393)
                     /locus_tag="PLK08_07260"
     CDS             complement(<1..2393)
                     /locus_tag="PLK08_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPS53332.1"
                     /translation="MKRRIFLRVILAVVCAAMIIFACAKLTARQMEKDTPAHKVSEKL
                     QFTQNERAFLKEHPVITVAIERPIPPIEFVDEYGNSIGLSVDYLRMLAERFGVEFSIR
                     NFASRSKALDAVLTGKVDMFAAIINSRENDNLVYTKPYISLPIFVYTQARQPYVRELS
                     ELDGRHVGVIKDASYVDSLAAEYPSLKLGYFLNADEAMPQLLAGKIYAFLGDMLTTGW
                     VITAEGYTAVKVSGGTDYKLEICMATRRSFVILRDILQKGLDSVSPQDAQVLQNRWTK
                     IEYENTTDRRALVGLLCVSFLMVMLFLIWNHRLRREIKRRQLVTDDLRENEDYLQSIF
                     DTMHSGVMLVDTHTFCIEQINDYACRLIGLPREKIVGQVCNDFICPAERGSCPVVAMG
                     GNMELAERSLIDGRGRMIPILKSVVRIKHDGREMFLETFTDMTQIKKFEMRMQADEDK
                     YRSLFEMSRDATIVSRNMRIIECNDAAVSMFGYANKQEMLTLGLAELSASHQAGGQST
                     TELLNELMDDAAAGILPLMEWQFAKKDGSTFNGEILASPAIIDGVPAVQVCIRDVSLR
                     KAIEVERTIFQQAIASAPVSVMITDGGGNIEYVNPFFTKVTGYELYEVLGKNCRCLQS
                     GRTSQDVYKNLWDTLGKGRVWSGYFYNRAKDGSEFSEYAQIAPIFDADGKVIHYLAVK
                     EDVTEKENLRKEVEYLAKFPSENPNPVWRISDEGILLYANDAGGEFMRLWNVSVGQPV
                     PDDFQQMVKKVCKSKEVHQISMAAGDKYYSISFAVVEGENYVNAYAQDISALKKVSE"
     gene            complement(2475..3347)
                     /gene="truB"
                     /locus_tag="PLK08_07265"
     CDS             complement(2475..3347)
                     /gene="truB"
                     /locus_tag="PLK08_07265"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPS53333.1"
                     /translation="MFGFISINKPQGPTSFDIVAGVRRMLSRKTKVGHTGTLDPFARG
                     VLVVCVNKATRLANYVQRSNKRYRAEITLGRVSDTDDVEGNITDFQTSQPLIPPDESA
                     VRNVLCEFTGQIMQIPPAHSAVHVGGQRAYVLARQGAEFEIAPRPVVIHEIKLLSYSW
                     PILTVAVYCGSGTYIRSLARDIGCRLNVGGYCSALQRTAVGMFDIESSVDFAELTADN
                     IAEKISMPLGALGLEIVTLADGQLESVSAGRSIVAPPGLKGREIALITADGRLAAIAD
                     MCGEGMLHPVRVFL"
     gene            complement(3355..>4039)
                     /locus_tag="PLK08_07270"
     CDS             complement(3355..>4039)
                     /locus_tag="PLK08_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS53334.1"
                     /translation="HTPEANTLAVVDYTVRMMIEMNRRFWPLSGYLSPEKFHSARSQL
                     FGRFMADLTLGIIGCGRIGSRVGRVADAMGMRVLYNDILPPEKLAVDYPAQSVDKDTL
                     YRESDIITIHLPVTELTRKIINAAAIAKMKNGVQFINAARGQCVDYDALGDALRSGKV
                     NAAVVDCHDPEPMPENYPLFGLENVILTPHVAACVPQAKVNMSWVVTDVIAVCNGQPP
                     KYPALPGSY"
BASE COUNT          969 a         1131 c          918 g         1021 t
ORIGIN      
        1 tcctcgctga ctttcttcag tgcggaaatg tcctgtgcgt atgcattgac ataattttca
       61 ccttcgacca cggcaaatga aatggaataa tatttatcgc cggcagccat ggatatctga
      121 tgaacttcct tcgatttgca cacctttttg accatctgct gaaaatcatc cggcacaggc
      181 tggccgaccg acacattcca taaccgcatg aattctccgc cggcgtcatt cgcatatagc
      241 aaaatgcctt cgtcggaaat tcgccatact ggattaggat tttcgcttgg aaattttgcg
      301 agatactcaa cttcttttct gagattttct ttttcggtga catcttcctt gacggcaaga
      361 taatgaatca ctttgccgtc ggcgtcgaaa atcggcgcta tctgcgcata ctcgctgaat
      421 tcactgccgt ctttagccct gttataaaaa tatccgctcc atactctgcc tttgccgagc
      481 gtgtcccata aatttttata aacgtcctgc gatgttctgc cggattgcag acatctgcaa
      541 tttttaccca gcacttcgta aagctcgtag cccgtcacct ttgtgaaaaa cggattgacg
      601 tattcgatat tgccgccgcc atcggttatc atcacggaaa caggcgcact ggcgatagcc
      661 tgctgaaata tcgtccgttc aacttctatt gccttgcgaa gcgaaacatc gcgtatgcag
      721 acctgtaccg ccggcacacc gtctattatg gccggactcg ccagaatttc cccgttaaac
      781 gtactgccgt cttttttggc gaactgccat tccatcagcg ggagtatgcc ggctgctgcg
      841 tcatccataa gttcgttcag caactccgtc gtggactgtc cgccagcctg atgcgaggcg
      901 gacaactcag ccagccccaa tgtaagcatc tcctgcttgt tcgcataacc aaacatgctc
      961 acagccgcat cattgcattc tattatcctc atgttacggg aaacaatcgt cgcatcgcgt
     1021 gacatttcaa acagcgaacg atatttatcc tcatccgcct gcatacgcat ttcgaacttt
     1081 tttatctgcg tcatatcagt aaatgtctcg aggaacattt ccctgccgtc gtgcttgata
     1141 cggacgacgg atttgagaat cggaatcatt ctgcccctgc cgtcaatcag actgcgttca
     1201 gcaagttcca tgttgccgcc catcgcaact acaggacagc tcccgcgttc agccggacat
     1261 atgaaatcat tgcatacctg gccaactatt ttttcgcgcg gcaggcctat cagccggcat
     1321 gcataatcgt taatctgctc tatgcagaac gtatgggtat ccacaagcat gacgccggaa
     1381 tgcatggtgt caaaaatgga ttgcagatag tcttcatttt cacgcagatc atcggtgaca
     1441 agctgccgtc gtttgatttc cctgcgcaga cgatgattcc atataaggaa cagcatgacc
     1501 attaaaaaac tcacgcatag caggcccacg agcgcccgtc tatccgtggt attttcatat
     1561 tcaatcttcg tccagcggtt ttgcagaacc tgtgcatcct gcggcgatac cgaatcaagg
     1621 cctttttgca aaatatcgcg aagaatcacg aaacttctgc gcgtcgccat gcatatctca
     1681 agtttataat ccgttccgcc tgagactttg acggcagtat agccctcagc cgttattacc
     1741 cagcctgttg taagcatatc gccgagaaag gcgtatatct ttccagccag aagttgaggc
     1801 atcgcctcgt ctgcattcag aaaataaccc aacttcagag acggatattc cgccgcaagt
     1861 gaatctacat aactggcgtc cttgatgacg ccgacgtgtc ggccgtcaag ttcggacagt
     1921 tcacgcacat aaggctgccg cgcctgcgtg taaacaaata tcggcagaga aatatagggt
     1981 ttcgtgtaaa caagattatc attttcgcgg ctgtttataa tcgcggcaaa catatccact
     2041 ttgccggtca ggactgcatc gagcgctttt gaccggctgg caaagtttct gatgctgaat
     2101 tcgaccccga atctttccgc cagcattctg agataatcca cggacagtcc gatgctgttt
     2161 ccatactcat cgacgaattc tatcggcgga atcggccgtt caatggcaac agttattaca
     2221 gggtgctctt tgagaaacgc acgttcattc tgcgtaaact gcaatttttc agaaactttg
     2281 tgagccggcg tatctttttc catttgcctg gccgtcagct tcgcacatgc aaatattatc
     2341 atcgctgcac aaacgacggc taaaatcacc cttaaaaata ttcgccgctt cataaggcca
     2401 aatccggaca tacgctcagt actccttttt gctacattat acacacaaag tcccccaatt
     2461 acatcaatat gccgttacag gaaaaccctt accggatgca gcatgccttc gccgcacata
     2521 tccgcaatgg cggcaagtct gccgtcggct gttatcaatg caatctcacg gcctttcagg
     2581 ccaggcggcg cgacgatact tctgcccgct gaaacacttt ccaactgtcc atctgccaac
     2641 gtcacgattt ccaacccaag cgcaccaagc ggcatggaaa ttttttcggc gatattgtca
     2701 gccgtaagtt ccgcaaaatc aacggatgat tcgatatcaa acatccccac cgccgttcgt
     2761 tgcaacgcac tgcagtatcc gccgacattc aggcggcagc cgatatcacg tgcaaggctg
     2821 cgaatgtacg ttccgctccc acagtaaacg gcgacagtca gaatcggcca cgaatacgac
     2881 agcagtttaa tttcatgtat cacgacgggc cgcggagcga tttcaaattc cgccccctgc
     2941 ctggccagca catacgcccg ctgcccacca acatgtactg cgctatgtgc aggcggaatc
     3001 tgcatgattt gccccgtaaa ctcgcacagc acattccgta cggcggattc atccggcgga
     3061 ataagcggct gagacgtttg aaaatccgtt atattacctt cgacatcgtc cgtatcgctc
     3121 accctgccaa gcgtgatttc agcacgatat cgcttgttgc tgcgttgtac gtaattcgcc
     3181 agccgcgtag ctttgttgac gcagacaacc agcactcctc tcgcaaacgg atcaagcgta
     3241 ccggtatggc cgactttggt cttgcgcgac aacatgcgac ggacgcctgc gacgatatca
     3301 aaactcgtcg gcccttgagg tttgtttatg gaaataaatc cgaacatgcg tttctcaata
     3361 actgcccggc agcgcgggat attttggcgg ctggccgtta cagacggcga taacgtcggt
     3421 aacgacccat gacatgttga ccttagcctg cgggacgcag gctgcgacgt gcggcgtaag
     3481 aatcacgttc tccaggccga aaagcggata attttcaggc attggctcag ggtcgtgaca
     3541 gtcaacgacg gctgcattga ctttgccgct tctgagcgca tcgccaagcg catcataatc
     3601 cacacactgg ccgcgtgcgg cgttgatgaa ctgtacgcca ttcttcattt tggcgatagc
     3661 ggcggcattg atgattttcc tggtaagctc cgtcaccggc agatggatgg ttatgatatc
     3721 ggattcacga tatagcgtat ccttgtcgac gctttgagca ggatagtcaa ccgccagttt
     3781 ttccggcgga aggatgtcgt tgtacagcac ccgcattccc atcgcatcag ccacacgccc
     3841 gactctggag ccgattcggc cgcagccgat gatgcccagc gtcaggtcag ccatgaatcg
     3901 gccaaaaagc tgactgcggg ctgagtgaaa tttttcaggc gaaagataac cgctcagcgg
     3961 ccagaagcgg cggttcattt ctatcatcat ccgcacggta tagtccacga cggctaacgt
     4021 gttggcttcc ggcgtatgg
//
LOCUS       DAONFI010000212         3964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_27585_length_3964_cov_4.909951, whole genome shotgun sequence.
ACCESSION   DAONFI010000212 DAONFI010000000
VERSION     DAONFI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27585_length_3964_cov_4.909951"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLK08_07275"
     CDS             complement(<1..578)
                     /locus_tag="PLK08_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011139654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPS53335.1"
                     /translation="MLTTIVILVYLFIIGYLGFLGFKNTQNATDYLLAGRQTHPFVMA
                     MSYGATFISTSAIVGFGGAAGMFGMSMLWLVFCNIFVGIFIAFVFLGGRTRSMGHHLD
                     AHTLPELMGRRYQSKFIQVFSGLMIFIFMPIYAGVVLIGGCEFITSHFGVDYDAALLV
                     FSVIIAVYVIAGGLKAVMYTDALQGTIMMVAMA"
     gene            complement(682..852)
                     /locus_tag="PLK08_07280"
     CDS             complement(682..852)
                     /locus_tag="PLK08_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53336.1"
                     /translation="MFGIDDISVILAFILCIASTILCLIYGIIQWNKGFEKPKTDDAV
                     WVKHEIEIDDKL"
     gene            complement(948..2264)
                     /locus_tag="PLK08_07285"
     CDS             complement(948..2264)
                     /locus_tag="PLK08_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPS53337.1"
                     /translation="MKPELENMIWDKAETLNRDDLAVLQLERLKRQAAHAMNVPFYKN
                     AFAKAGVTPDDIKSLDDIRRLPFTTKADMRENYPLGLMAVSRDDIARIHGSSGTTGKP
                     TFVAYTKEDMKTWSNLVARFLVAGGLRAKHIAQVAFGYGLFTGGFGLHYGIETVGAAV
                     VPASAGNTPRQLMLLEDLGVDVLICTPSYALNLAEIAAEMNCIDRLKLKFAHFGGEPW
                     TEDMRRQIEDMLQIYAFNNYGLSEVIGPGVSGECIYRRGMHFQEDHFIVECINPKTLE
                     PVAAGEKGELVITSLTKQAMPVIRYRTRDIASIDDSPCQCGRTGRRMSRVIGRTDDMM
                     IIRGVNIFPSQIEEAILRIEGTAPHYLIELSRPGALDIITVKVEILPNIFSDRMSEMQ
                     NLHDRLVREIQNVIGVRPNVELVKPQSLERFVGKAKRVVDNRKLAD"
     gene            complement(2292..2729)
                     /locus_tag="PLK08_07290"
     CDS             complement(2292..2729)
                     /locus_tag="PLK08_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPS53338.1"
                     /translation="MKIKQLSVFLENKPGQLNIPCKALASAGLNILTLSLADTHQFGI
                     LRLILEEPDKGKEALEAAGCVVNVTEVVAVTVPDRPGGLAEILDLIEKAGINLEYMYA
                     FTARKKDQAILVFRFEDPETAIEVLQKNGISVINRVELFDLAK"
     gene            complement(2740..>3964)
                     /locus_tag="PLK08_07295"
     CDS             complement(2740..>3964)
                     /locus_tag="PLK08_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPS53339.1"
                     /translation="TVSLAYERQALFRSRMEERGLTPASIRSLDDIALLPFTIKADLR
                     DTYPFGLFAKPMSEIVRLHASSGTTGKPIVVAYTKKDIEVWADAIRRAFICAGLGEHD
                     IIQNSYGYGLFTGGLGAHYGGEKLGATVIPTSGGNTDRQIMVMKDFGVTAICCTPSYL
                     AYLLERAEELGINVQELPLKAAILGAEPWAWETGRRLEKMGGLRVFDIYGLSEIVGPG
                     VGMMCGEQDGLHIFEDYFYPEIINPDTCEVLPDGQEGELVLTTLAKEAMPMIRYRTRD
                     ITSIITQPCPCGRTCRRINRISRRSDDMMILRGVNVFPSQIETALMAVQGTLPHYQIV
                     LTTRHGLDQMEVQVEVTAEHFGDRIRELEDLKVKLAKSIERTVGIRIDVTLVKPQTIE
                     RSQGKAKRIVDLRKK"
BASE COUNT          796 a         1043 c         1101 g         1024 t
ORIGIN      
        1 gccatggcaa ccatcattat cgtaccctgc agggcatcgg tatacatgac cgccttcaag
       61 ccgccggcaa tgacgtatac cgcaatgatt acgctgaaaa ccagcaacgc agcatcgtaa
      121 tccacgccga aatgagacgt tatgaactcg cagccgccaa tgagcaccac gccggcgtaa
      181 atcggcatga aaataaatat catcaagccg gaaaaaacct gtatgaactt actctggtaa
      241 cgacggccca tcaactccgg cagcgtgtgc gcgtcgaggt ggtggcccat gctgcgtgtt
      301 ctgccgccga gaaaaacgaa cgctatgaaa atgccgacga aaatattgca gaacaccagc
      361 cacagcatgc tcatgccgaa catgccggct gctccgccga agcccacgat tgcacttgtc
      421 gatatgaacg tcgccccgta gctcatcgcc attacgaacg gatgagtctg gcggccggcc
      481 agcagatagt cggtggcatt ctgcgtattt ttgaatccca gaaaacccag ataacctatg
      541 atgaagagat aaacgagaat tacgatagtt gtaagcatga ttttcttttc cgcctttaat
      601 taagctgaaa gtatacgtcg tcaggctgac aggcaacacc tgatacacgg gttttaaccc
      661 gtagagattc tccactgttt gttatagttt gtcgtctatt tcgatttcat gtttgaccca
      721 tacggcgtcg tcggttttag gtttttcgaa acctttattc cattgaatga tgccgtatat
      781 tagacagaga atcgtactgg cgatgcatag gatgaacgcc agaataaccg atatgtcgtc
      841 aattccgaac attttcatat atcctttgcg atttttgtgt tttttgggaa aactgcgaac
      901 cgttttcagc tatcgttcgg tagcgtctga agtattagta ttcaaagtca gtctgcgagt
      961 tttctgttat cgacgacccg tttggctttg ccgacgaatc gttcgagtga ttgcggcttt
     1021 acgagttcaa cgtttggccg tacgccgatg acattctgaa tttctcgcac cagccggtca
     1081 tgcagattct gcatttccga catcctgtcg ctgaagatgt ttggcaggat ttcaaccttt
     1141 accgtgatga tgtcgagggc accgggccgc gaaagttcta taagataatg cggcgcagta
     1201 ccttcgatgc ggaggatagc ctcttctatc tgcgacggga aaatatttac gccgcgtata
     1261 atcatcatgt cgtcggttct gccgatgact cggctcatgc gtctgccggt tcggccgcac
     1321 tggcatggcg agtcgtctat gcttgcgata tcgcgcgtgc ggtaacgtat cacgggcatg
     1381 gcctgtttgg tcagcgacgt tatgacgagt tcgccttttt cgccggcggc tacgggttcg
     1441 agtgttttgg ggttgatgca ttccactatg aagtggtctt cctggaagtg catgccgcgg
     1501 cggtatatgc attcgccgct gacgcccggg cctattactt cgcttaagcc gtagttgttg
     1561 aaagcgtaaa tctgcaacat gtcctctatc tgccgtcgca tgtcttccgt ccacggttcg
     1621 ccgccgaagt gggcgaactt gagtttgagg cggtcgatgc agttcatttc tgcggcgatt
     1681 tccgccagat tcagcgcgta gctcggcgtg catatgagga catcaacgcc gaggtcttcc
     1741 agcagcatga gttgtcgcgg cgtattgccg gccgacgccg gcactacagc tgcaccgacg
     1801 gtttctatgc cgtaatgtaa tccaaacccg ccggtaaaaa ggccgtaacc gaaggctacc
     1861 tgagcgatgt gtttggctct gaggccgccg gctacaagga aacgggctac caggttcgac
     1921 cacgttttca tgtcttcttt cgtgtaggct acgaacgtgg gtttgccggt cgtgccgctt
     1981 gagccgtgta ttctggcgat gtcatcgcgg ctgacagcca tgagtccaag cgggtagttt
     2041 tcccgcatgt cagccttggt tgtgaacggc agccgccgga tgtcatcgag cgattttatg
     2101 tcatctggag ttacgccggc ttttgcgaag gcgtttttat aaaagggcac attcatggca
     2161 tgggcggcct ggcgtttgag ccgctcaagc tgaagtacgg caaggtcgtc gcggttgagc
     2221 gtttcagcct tgtcccatat catgttttca agttcaggtt tcattttatt gtccggactc
     2281 tgttgcgtgc gttactttgc gaggtcgaaa agctccacgc ggttgattac actgatgccg
     2341 tttttctgca ggacttcgat tgcggtttcc gggtcttcaa agcggaatac gaggatagcc
     2401 tggtcttttt ttcgtgccgt gaaggcatac atgtattcca ggtttatgcc cgccttttcg
     2461 atgaggtcca ggatttccgc caggccgccc ggcctgtccg gaacggtgac ggcgacaacc
     2521 tcggtcacgt tgacgacgca gccggcggct tcgagcgctt ccttgccctt gtccggctct
     2581 tccaggatta gccgcaggat tccgaactga tgcgtgtcag ccagcgaaag cgtgaggatg
     2641 ttcaggcctg cactggcgag ggctttgcac gggatgttaa gctgcccggg cttgttttcg
     2701 agaaaaactg atagctgttt tattttcatg gctgactcct tatttctttc tcaaatcgac
     2761 gattcgctta gcctttccct ggctgcgttc gatagtttgc ggttttacca gcgtcacgtc
     2821 gatgcgtatt ccaactgttc gttcgattga tttggcgagc tttactttca aatcttccag
     2881 ctcgcggatt cggtcgccga agtgttccgc ggtgacttcc acctgcactt ccatctggtc
     2941 gaggccatgc cgcgtggtta gaactatctg ataatggggc agtgtgccct ggacagccat
     3001 cagcgcggtt tcgatttgcg acgggaatac gttcacgccg cggagaatca tcatgtcgtc
     3061 gctgcggcgg ctgatgcggt tgatgcgccg gcatgtccgg ccgcaggggc agggctgtgt
     3121 gatgatggat gttatgtcgc gagtcctgta gcgaatcatc ggcatggctt ccttggccag
     3181 cgtggtcagg acgagttcgc cttcctggcc gtcgggaaga acctcgcacg tgtcgggatt
     3241 tatgatttcg gggtaaaaat aatcctcgaa gatgtgcagg ccgtcctgtt cgccgcacat
     3301 catgccgacg ccgggcccga ctatttcgct aaggccgtaa atgtcgaaca cccgcaagcc
     3361 gcccattttc tccagacgtc tgcctgtttc ccacgcccag ggttcggcgc cgagtatcgc
     3421 agccttgagc ggcagttcct gcacgtttat gcccaactct tccgcgcgtt cgagcagata
     3481 ggccagataa ctcggcgtac agcatattgc cgtcacgccg aagtctttca tgaccattat
     3541 ctgacggtcg gtattgccgc cggatgtggg tatgaccgtc gcgccgagtt tttcgccgcc
     3601 gtaatgtgcg ccgaggccgc cggtaaaaag gccgtagccg taggaattct gtatgatgtc
     3661 gtgttcgccg aggccggcac atatgaacgc acggcgtatg gcgtcggccc agacctcgat
     3721 gtccttcttc gtgtaggcta cgactatggg tttgccggtc gtgccgctcg atgcgtgcag
     3781 ccggacgatt tcagacatgg gttttgcaaa caggccgaaa ggatacgtat cgcgaaggtc
     3841 ggccttgatg gtgaacggca gcagggcgat gtcgtcgagg ctgcggatgc tggccggcgt
     3901 cagaccccgc tcttccatgc ggctgcggaa aagcgcctgc ctctcatacg ccagcgatac
     3961 cgtc
//
LOCUS       DAONFI010000213         3954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_27682_length_3954_cov_4.144396, whole genome shotgun sequence.
ACCESSION   DAONFI010000213 DAONFI010000000
VERSION     DAONFI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27682_length_3954_cov_4.144396"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1265)
                     /locus_tag="PLK08_07300"
     CDS             complement(87..1265)
                     /locus_tag="PLK08_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPS53340.1"
                     /translation="MKFGHKKSGWVWAKALPIIFAVAVVVLVIKFYPNSKNEQAVLES
                     PPVTVEVMTINPDSSVRDVITLPGHVEPNKVVDVAAEVAGRVDAHAGKADRLGKGMEI
                     TRGPASAGVVNEGDYVKQGQPIVYINTDILLAQCEKTLAEYEFQQQEFKRVSELYGKD
                     VATRTELETTKMNRDMAKAAYDLCRANLERTVVVAPITGRVNSLPVEAGEYVQPGNLV
                     AEFVDMDTAKIVVEVPERDISYMKLGQKQNITVGTFSPTTLQGKISYISDLADEKTHT
                     TRVEIVVDNKNRALRSGQFVKVELLRRELESPIMAPLGVIIPMEQGHAVYVVENDTAQ
                     RRDVELDYSLLRGQFIRISKGLKKGDRLIVAGQRLVGPGQKVRIRSTGMDTLLKADQE
                     "
     gene            complement(1262..1936)
                     /locus_tag="PLK08_07305"
     CDS             complement(1262..1936)
                     /locus_tag="PLK08_07305"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPS53341.1"
                     /translation="MISLDIFRDDKKMFYNVKDKKDLNSRKMELLDAAERVFSRHGYA
                     ATRMDDIAAEANVSKGSVYNYFRSKQEIFKEVFLYSQTKDSEIYDEIMNSPTLPVREK
                     ILKIIQTAFTRFDTKSHIGKLLMEFWALAGREETRGMNDVINELYRKYIKVIEDIIAQ
                     GQSCGELSRFTTPNMSARMLVAFLDGLGLQKILGIGGDWSLQDYAEISNTIDRIMGPI
                     GSVKNI"
     gene            2123..3415
                     /gene="lysA"
                     /locus_tag="PLK08_07310"
     CDS             2123..3415
                     /gene="lysA"
                     /locus_tag="PLK08_07310"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPS53342.1"
                     /translation="MDKFNYKDGKLYCEDVPVAEIAEKIGTPAYIYSANTIREHYRKI
                     EAAFSELDTTICFSIKSLANLSIMKLLKNLGSGFDVVSGGELARVQAIGGDVKKVVYA
                     GVGKTDREISEALQAGIGWFNIESEMEFENLARLAGEINCTAHAALRINPDVYDPKTH
                     AYTTTGKKETKFGVDIDRAGAFFEKYGKNPNVRLDSIHLHIGSPIYSPQPYIAAINKT
                     LAFVKDMRAKGLEINTLDLGGGFAADYEEGASPLAIDYAAAIVPLLKGQGLKLILEPG
                     RHIACNAGILLMKVLYTKKGGEKNFIIVDAAMTDNIRPALYEARHFIYPSKLEADEFP
                     PNRRFDFQPAKGIKVNVVGGVCESADVLAKDRVLPPVKRGDLLAMFSAGAYCFTMSSQ
                     YNTRPRAAEVLVDGEKFRVIRKREDYRDIFATELDAIK"
     gene            3754..>3954
                     /locus_tag="PLK08_07315"
     CDS             3754..>3954
                     /locus_tag="PLK08_07315"
                     /inference="COORDINATES: protein motif:HMM:NF036305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53343.1"
                     /translation="MDAATLKTKMDAAGYAKLQSLNCPKLIEFISTSAELCKPEKIVL
                     CNGTKEEYQSLMNEMIKEGLAKK"
BASE COUNT          995 a         1037 c          924 g          998 t
ORIGIN      
        1 atcgtcccca ttacgattat gagcacgacc atgaccttga cggtcgtccg gttttttatt
       61 gcgacatcag agagaatcat gcggcgttac tcctggtcag ccttgagcag cgtatccatg
      121 ccggtcgaac ggatacgcac cttctggccc ggcccgacga gacgttggcc ggcgacaata
      181 agcctgtcgc cctttttaag gcctttcgag atacggatga attggccgcg cagtagcgaa
      241 taatccaact cgacatccct gcgctgcgcc gtgtcgtttt ctacgacata aacggcatgg
      301 ccctgctcca taggaataat cacgcccaga ggagccatta tcggcgactc cagttctctc
      361 cgcaacagtt ccaccttgac gaattggccg ctgcgaagag cccgattttt attgtcgacc
      421 acaatctcga cccgcgtggt atgcgtcttt tcgtcggcaa gatcactgat gtaggatatt
      481 ttaccctgca gtgtcgtcgg gctgaacgtg ccgacggtta tgttctgctt ctggcccaat
      541 ttcatatagg atatgtcccg ttccggaact tcaacgacga ttttcgcggt atccatatcg
      601 acaaattcag ccaccaagtt tccaggctga acatattcgc cagcctccac cggcagcgag
      661 ttgacgcggc cggtaatcgg tgcaaccacc acagtccgct caagattcgc cctgcaaagg
      721 tcatacgccg ccttagccat gtccctgttc atttttgtgg tttcaagctc cgtcctggta
      781 gccacgtctt taccgtacag ctctgacaca cgtttgaatt cctgctgctg aaactcatat
      841 tccgccagcg ttttttcaca ttgtgcgaga agtatgtcgg tattgatata aacaatcggc
      901 tggccctgtt tgacgtagtc gccttcattc acgacgccgg ctgaggctgg gccgcgggtt
      961 atttccatgc ctttgccgag ccgatcggct ttgccggcat gggcgtcaac ccggccggcg
     1021 acttcggcgg ctacgtccac aaccttgttc ggctcaacat ggccgggcag agttatgacg
     1081 tccctgacgc tgctgtcggg atttattgtc attacttcaa ccgtgaccgg cggcgactca
     1141 agcacggcct gctcattctt actgttcggg tagaatttta tcaccagaac cactacggcg
     1201 acggcaaaaa ttatcggcag agctttcgcc catacccaac ctgatttctt gtgaccgaat
     1261 ttcatatatt tttaacgctc cctatgggtc ccattatcct gtctatagta ttcgatattt
     1321 ccgcgtaatc ctgcaaactc caatcgccgc cgatgccaag gattttctgc aatccaagcc
     1381 cgtcgagaaa cgcaaccagc atccgtgccg acatattcgg cgtcgtaaac cgtgaaagtt
     1441 cgccgcatga ttggccctgt gcaataatat cctcaataac cttgatatat ttccgataaa
     1501 gctcgtttat gacatcattc atgccgcgag tttcttccct gccggctaac gcccagaact
     1561 ccataagcaa tttgccgata tggctcttgg tatcaaaacg ggtgaacgca gtttgaatta
     1621 tctttaaaat tttctcgcga acaggcagcg tcggagaatt cattatttca tcataaattt
     1681 cactatcttt cgtctgggag tacaggaaaa cctccttaaa aatctcctgc ttgctcctga
     1741 aatagttgta gacactgcct ttgcttacgt tggcttccgc ggcaatatcg tccattcgcg
     1801 tagcagcgta cccatgcctg ctgaacacac gctcggcggc atcgaggagt tccatcttcc
     1861 tggaattcaa atcttttttg tctttgacgt tataaaacat ttttttatcg tcgcgaaaaa
     1921 tatctaagct tatcatgtca cagccaacat cttacatttc atgcagcagc aacattttcc
     1981 caaaaatttt catcaatcgg tttttatata ttatattcgt tgactgaccg gtcagtcaac
     2041 ggctatgttt tatgctttta tggcggtttg gccggaaaat aggcttgtga tttttatcca
     2101 aatggttatc tttagtcaca ttatggataa gtttaactat aaagacggca agctgtactg
     2161 cgaagacgtg ccggttgcag agatagctga aaaaatcggc acgccggcat acatatattc
     2221 cgccaacact atccgcgagc attatcgcaa gatagaagcg gcgttttcgg aattggatac
     2281 gaccatatgc ttttcaatca aatcattagc caacctcagc ataatgaagt tgctaaaaaa
     2341 cctcggcagc ggttttgacg tcgtcagcgg cggcgaactg gcccgcgtac aagccatcgg
     2401 cggcgacgtc aaaaaggtcg tttacgccgg agtcggcaaa acagaccgtg aaataagtga
     2461 agccctgcaa gccggcatag gatggttcaa tatcgaaagc gaaatggaat tcgaaaacct
     2521 cgccaggcta gccggcgaaa ttaattgcac cgcccatgca gccctgcgaa tcaatcctga
     2581 cgtttacgac cccaaaaccc atgcctacac caccacgggc aagaaggaaa ccaagtttgg
     2641 cgtggatatt gaccgtgcag gagcattttt tgagaaatat gggaaaaatc cgaacgtccg
     2701 gctggacagc attcacctgc atatcggttc gccgatttat tctcctcagc cgtacatagc
     2761 tgctatcaac aagacgttgg cattcgttaa ggatatgcgt gcaaaaggtt tggagataaa
     2821 cacgctcgac ctgggcggcg gttttgctgc cgattacgag gaaggcgcaa gcccactggc
     2881 gatagattac gcagccgcca tcgtaccgct gctcaaaggc cagggattga aactcatact
     2941 cgaacccggc cgccacatcg catgcaatgc cggcatactt ctgatgaagg tgctgtatac
     3001 caaaaaaggc ggcgagaaaa atttcatcat cgtcgatgca gccatgacgg acaatatccg
     3061 ccccgctctc tacgaggcaa gacatttcat atatccctct aagctggaag cggacgaatt
     3121 ccccccaaat cgccgtttcg atttccaacc agccaaaggc ataaaagtga acgtcgtagg
     3181 cggcgtatgc gaatcggcgg acgtgctggc gaaagaccgc gtgctgccgc cggtaaaacg
     3241 cggcgacctg ctggcgatgt tctccgccgg cgcatactgt ttcaccatgt ccagccagta
     3301 taacacccgt ccgcgtgcgg ctgaagtgct ggtggacggc gaaaaattca gagtcatacg
     3361 caaacgggaa gattacagag atatcttcgc cactgagctt gatgccataa aataatttta
     3421 cacacgcggc aaatggcgga ttttatcaga tggctgaagg ttttacgttg ttttccaaaa
     3481 ttttcccaaa ttgcctgccg cctgtcgaac tgcatcggca aaaaaatatc tgattaagtt
     3541 cttgacatct tgcaggcgtt gttgtagtta atatccctcg aaagtttggc ctgcggtgaa
     3601 ggtgtgcaaa tgccgcaggg ctctctttgc cggtccgcga aactggccgg ttctgaaatt
     3661 gcgtataaat tccagttttt aacatggcgg tttgacactg cgcttaaaaa caggaatcag
     3721 gccaatataa acctttttat cacgaggagt gtcatggacg ctgcaacgct gaaaacaaag
     3781 atggacgctg cgggttatgc gaagctgcaa agcctcaact gcccgaagct gattgaattt
     3841 atttccacca gtgccgaact gtgcaagccg gagaaaatcg ttctctgcaa cggcacgaaa
     3901 gaggaatatc agtctctgat gaacgaaatg ataaaagaag gcttggccaa aaaa
//
LOCUS       DAONFI010000214         3949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_27738_length_3949_cov_4.883924, whole genome shotgun sequence.
ACCESSION   DAONFI010000214 DAONFI010000000
VERSION     DAONFI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27738_length_3949_cov_4.883924"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /gene="dnaE"
                     /locus_tag="PLK08_07320"
     CDS             complement(<1..1756)
                     /gene="dnaE"
                     /locus_tag="PLK08_07320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPS53344.1"
                     /translation="MDGFVHLHVHSHYSLLDGAGTCERLVNAAKSRGMTSLALTDHGN
                     LFGAMDFYNTAKAAGVKPILGMEAYISPTNRRDKSMGSQREACYHLILLAMNAKGWLN
                     LKRLSSRAFLEGFYYRPRVDRELLAELNEGIICASACLGGEIPSALLAGNYEKALHLA
                     GEYAEIFGKDRFFIEIQNGGINEQAQVNPDLMKIAGEIGVGVIGTNDVHFISREDKQA
                     HEVLCCVSTGKTLDEERQAIAAGKKKAAYTDELYLKSPEEMRSTLREFPQTCDNTLKI
                     AEMCDLEVVNEAHLPVFPLPQGMTDDEQLANLAWQGLGERFGGSDKIPEEYRNRMERE
                     LHVIRDKGYSSYFLICHDFVNYARSNNIPASPRGSGLSSLLCYSLRIAEIDPLKYGLL
                     FERFTDPQRKEAPDLDIDICQEGRQDVIRYVREKYGHVAQIITYGTLKAKAVLKDVGR
                     TMGFTPQEMDAITKLVPDGPKITLEKALSAEPDLRKLYDSDSRIKELFEYGKRLEGLC
                     RHTGVHAAGVIIADVPLEDVVPLYCQGGNKEEVITQWDGPTSEKAGLMKMDFLGLRTL
                     TIIQRARELVMAKTGRDID"
     gene            complement(1760..3310)
                     /locus_tag="PLK08_07325"
     CDS             complement(1760..3310)
                     /locus_tag="PLK08_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53345.1"
                     /translation="MKKSGIGQSGLAVMILLAGGFLMMAGVAGCSDNGTTVKVETTPS
                     AAKVFIDGKFVGLSPCECFIRDSQPDDLWEQHTVEVKAKGFESQSRRLRYRTGAAWLP
                     DKIEFVMSKSLLIDEPENASRDSKASAEPSPTDTRVSRQNITNKKSAVVETPKEVTYR
                     PAEAERIKKFKAAAPWTKAAPINDDNLQASQLSKKSDEKIQTTLAARHAEMEKTVVAA
                     KGSSLITAPTLPELEPTNSNTATKESSLAVTHSKTDEPQAAASVVPLTRPRLAPLPKQ
                     VTITKKPSSPQFIGSVPWRNISSTNRQHEIELISAPVDRHVIPAQPTENSKTGEKTSC
                     QQAAPWLQAGGLGIEKSQPENAHPITQPQDTRMLSCDIRIARVSDGVVLHQASGVDTY
                     ANMADLAHDLVVELAAYVPDDSSVAIFCFHNRRNTNSGRLVADKMLEAVVDEVLKSPS
                     LKFIKTVNIRDALGSETSLEQSKQLTKPRFYPLLKNAEYVIIGGVSLFDTFADLSTKP
                     TRETGKED"
BASE COUNT          797 a          998 c         1099 g         1055 t
ORIGIN      
        1 ggtcgatgtc cctgccggtt ttagccatga cgagttcgcg ggcgcgctgt atgattgtga
       61 gcgtgcggag gcccaggaag tccattttca tgaggccggc tttttcgctc gtagggccgt
      121 cccactgggt gatgacttct tccttgttgc cgccctggca gtacagcggc acgacgtctt
      181 ccaggggaac gtcggcgatg atgacgccgg ctgcgtgtac gccggtgtgc cgacacaacc
      241 cctccagccg tttgccgtat tcgaagagtt cctttatacg gctgtcggag tcgtaaagtt
      301 tcctgaggtc gggctcagcc gagagagcct tttcgagcgt gattttcggg ccgtctggca
      361 cgagtttggt tatcgcgtcc atctcctgcg gcgtgaaacc catcgttctg ccgacgtcct
      421 tgagtacagc tttggccttg agcgttccgt atgtgataat ctgtgcgaca tggccgtatt
      481 tttcacgcac atatcgtatt acgtcctgcc ggccttcctg gcagatgtcg atatcaaggt
      541 cgggcgcttc cttacgttgc gggtctgtga aacgctcgaa aagcaggccg tatttcagcg
      601 ggtctatctc ggcgatgcgc agcgaataac acagcagcga actgaggcca gaacctctgg
      661 gcgacgccgg aatattattg ctgcgggcgt aattcacaaa atcgtggcat atgagaaaat
      721 atgaactgta acctttatcg cggattacat gaagttcccg ttccatgcgg ttgcggtatt
      781 cttcgggaat tttgtcgctg ccgccgaacc tctcgcccaa gccctgccat gcgagattgg
      841 cgagctgttc gtcgtcggtc atgccctgcg gaagaggaaa aaccggcaga tgggcttcgt
      901 tgacaacttc caggtcgcac atttcggcga ttttcagcgt gttgtcgcag gtttggggga
      961 attcacgcag cgtcgaacgc atttcttcgg gcgatttgag atataattcg tcggtgtacg
     1021 ccgccttttt tttgccggct gcgattgcct gtcgttcttc gtcgagcgtt ttgccggtcg
     1081 aaacgcagca tagcacttcg tgagcctgct tgtcttcccg gctgatgaag tgtacgtcgt
     1141 tcgtacctat tacgccgacg ccgatttctc cggcgatttt cataaggtcg ggattaacct
     1201 gtgcctgttc gtttatgccg ccgttctgga tttcgatgaa aaatctgtcc ttgccgaaga
     1261 tttcagcgta ttcgccggca aggtggaggg ctttttcgta attgccggct aacaacgcgc
     1321 tggggatttc tccgcccagg cacgcgctgg cgcagatgat gccttcgttg agttcagcca
     1381 gcagttcgcg gtcgacgcgg ggacgatagt aaaaaccttc cagaaatgcc cggcttgaga
     1441 gacgtttgag attcagccag cctttggcgt tcatggccaa cagtatgagg tgatagcacg
     1501 cttcccgctg tgaacccatg gatttgtctc tgcggttcgt cggagagatg taggcttcca
     1561 tgccgaggat gggtttgacg ccggcggctt tggcggtgtt gtagaagtcc atcgccccga
     1621 agagattgcc gtggtctgtc agtgccagcg acgtcatgcc tcgcgatttc gccgcattca
     1681 ccagccgttc gcacgtgcct gcgccgtcca gcagcgaata atgcgaatgg acgtgaagat
     1741 gtacgaaacc gtccatgaat cagtcctctt ttccggtttc gcgagtcggc tttgtcgaca
     1801 agtccgcaaa agtgtcgaac agcgatactc cgccgattat gacgtattcg gcgtttttga
     1861 gcagcgggta aaatcgcggt ttcgtgagct gcttggactg ctccagagaa gtttcactgc
     1921 caagcgcgtc acggatattg actgttttga tgaatttaag gctgggcgat ttaaggacct
     1981 cgtccacgac ggcttcgagc attttgtcgg ctacgagtcg gccgctgttg gtattgcgcc
     2041 tgttgtgaaa acagaagatt gccaccgacg aatcatccgg cacatacgcc gccaattcca
     2101 ccaccaggtc atgtgccagg tccgccatgt ttgcatacgt gtctacgccg gatgcctgat
     2161 gcaacaccac tccgtcagat acgcgcgcta tgcggatgtc acacgaaagc atgcgtgtgt
     2221 cctgcggctg tgtgattggg tgtgcgtttt ccggttgact tttttctata ccaaggccgc
     2281 cggcttgcaa ccagggtgca gcctgctgac aggatgtctt ttcgcccgtc tttgaattct
     2341 ccgtcggctg ggccggtatg acgtgcctgt cgaccggtgc ggagatgagt tctatttcat
     2401 gctgccggtt tgtggaagat atatttctcc acggtacgct gccgatgaat tgcggcgaag
     2461 acggtttttt cgttatcgta acctgcttgg gcaatggggc aagacgcggg cgtgtcagcg
     2521 gtacgacgga tgcggcagcc tgcggctcat cggttttgga atgggtgacg gcgagggagg
     2581 attcttttgt agccgtgttc gaatttgtcg gctcaagttc cggcagtgtc ggggcggtta
     2641 tcaacgaaga accctttgcg gcgactacgg ttttttccat ctcggcgtgc cgcgcggcga
     2701 gggttgtctg tattttctca tcagattttt ttgataattg ggatgcctgc agattgtcgt
     2761 catttatggg ggcggctttt gtccacggtg cggcggcttt gaatttcttt atacgttcgg
     2821 cctctgccgg tctgtacgtc acttctttag gcgtttcgac gacagctgat tttttgttcg
     2881 tgatattctg tcgactgacg cgagtgtcgg ttggtgacgg ctcggcagag gctttcgaat
     2941 cgcggctggc gttttccggc tcatcaataa gaagcgattt gctcatgacg aattctattt
     3001 tgtccggcag ccatgccgcg ccggttcgat atcgcaacct gcgagactgc gattcaaaac
     3061 ctttggcctt gacctcgacg gtgtgctgtt cccacaaatc gtccggctgg gaatcccgaa
     3121 taaagcattc gcacggacta aggcccacaa atttgccgtc gatgaacacc ttcgccgccg
     3181 acggcgtagt ctccaccttt accgtcgtgc cattgtcact gcagccggcg acgcctgcca
     3241 tcatcaaaaa gccgcccgcc agcaatatca ttacagccag gccggattgt ccgattcccg
     3301 attttttcac gccgcatatt ttacccgctg aatttcgcgt tacaaggact aaaaattaca
     3361 ggttttcagg aatacaatgt taacgacagt cgttacaggc ggcaacgccg gcgccggcag
     3421 ccttttgctg gatgaatacc gaatgaatat aagacaaatc tgaaaaactt ttgaggaata
     3481 aatatgaaaa ccggcgagtc ttttatttgc cgagtcttac agaaaaaaag agcgacgtcc
     3541 aggtggaggg agacctgaac gccgcgttag ctgcgtccca atcagccagg tggagggaga
     3601 cctgacttac atctctttcg ggtgcagcgg acaacgctcc tatataaggg aataacccga
     3661 aagagtcaag acaaaaaact tttttttaga aataatgtga tttatttcac aatatggaat
     3721 ttttgcaaat tgtttcaaaa tgaccccaaa aaccccattt ttatcacaat cgcttacaag
     3781 gccctatatt ggttggcggc tgtattgtaa gcctgtttag cctcatttgt aaggtcaatc
     3841 cgactgcaat gttacatgat gagatagccg gtattccggc ggcataatta tggagtttta
     3901 gagcatgtta aatttgaaat tgcgccttgt tgcgtcgttg aggacgagg
//
LOCUS       DAONFI010000215         3942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_27791_length_3942_cov_5.557242, whole genome shotgun sequence.
ACCESSION   DAONFI010000215 DAONFI010000000
VERSION     DAONFI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27791_length_3942_cov_5.557242"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /gene="bamD"
                     /locus_tag="PLK08_07330"
     CDS             complement(<1..1189)
                     /gene="bamD"
                     /locus_tag="PLK08_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPS53346.1"
                     /translation="MNSFQKRQNDAATPAIGVLIIIMLVTMSLACVSVCADGETMVWN
                     GKNWVSQKPPARGTPQGDINYIRELIEEGRYKTAIKEADVFLVNYAGTPACEEAMNLA
                     GQACMDQGEYWAAYQRFEDQVAAYPNGAFFDRALDREYKIAEAFLNGRKRRALKILKL
                     SAKDEGIDILLRISSHSPNSELGQRSLLRVADYHFNNRDYKDAINTYEDFEKQYPRSS
                     RRAYAMLQTAKAYVNSYKGVSWDDTPLMDAKKKYEVFAAAYPRMAKQENVPEILKQIH
                     EIRAHKVYNIADFYRRVHRPTAAVFYYQRTVDEFPGTEWAQTAREMLDMLAPDKTPEQ
                     RRQAPADENILKMPVRKYGLTQEITNPPAQPVQNSAAAQNDSASQSSPHSARDVIMPL
                     EKMD"
     gene            complement(1729..2289)
                     /locus_tag="PLK08_07335"
     CDS             complement(1729..2289)
                     /locus_tag="PLK08_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53347.1"
                     /translation="MKSAKVLLAGIGLVTLLISSGCVSRIIKEGVGTATGAKGGYQEL
                     DNPGRLAGRNFQLGKFEDVNKRIIPGELLRELPAAMARQMAEDNIPATGAADETVVIN
                     GRIIYYEKAGASGQLFGPLEEVVAEVTLVDKASGRILGQAMCVGRSQESVNQGVKEKA
                     QGLAKGICSWIVKAADIQKKKEKEKS"
     gene            complement(2478..3119)
                     /locus_tag="PLK08_07340"
     CDS             complement(2478..3119)
                     /locus_tag="PLK08_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53348.1"
                     /translation="MMIQLTRKQKAISLMTVGGIVLIAVVVGAFALFRSDSQSPEVTP
                     ANATNVVKFIASDNFNKLDGVKQKEFLDQMRENPEMRDAMFSQRQSLTEKERENLRRN
                     MGNMFRQQMQDRMNRYFALKTAEEKNEMLDEMIADMEKHRAEMDKRRKEDTDKKPESQ
                     PDDGKSRESHMREHMEKSSPQERAQMHQFMSDMMARMKARGITPPGPPGGPRH"
     gene            complement(3202..3876)
                     /locus_tag="PLK08_07345"
     CDS             complement(3202..3876)
                     /locus_tag="PLK08_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53349.1"
                     /translation="MIIVNHILQKLSGIRPISLTKRQKIIVLSSAAGGLVLLLGAAIL
                     LACIRTDVPPAATVQNAPAVAKFIASPQFAKLDSDAKTEYVRQINEDSQIRLAIAEQR
                     ENLSDDERSNMRQNMRTIRQQQMQERMDKYFSMKTPEEKNKILDEILADMQKHSQARP
                     QQPNATPANNNRPQANDNASRQQRRMRFRETASPQQHAQRRQFHADLIARAKALGVPL
                     PWRRHR"
BASE COUNT          888 a         1054 c          904 g         1096 t
ORIGIN      
        1 aatccatttt ttccagcggc ataattacgt cgcgcgccga gtggggcgat gactgcgacg
       61 cagaatcatt ctgcgcggcg gctgaattct gtaccggctg agccggcgga ttagttattt
      121 cctgcgtcaa gccgtatttc cgcaccggca ttttaaggat attctcatcg gcaggcgcct
      181 gccttcgctg ttccggcgtt ttatccggcg caagcatatc gagcatttcc ctggcggtct
      241 gagcccactc cgtaccggga aattcgtcga ccgttcgctg gtaatagaaa acggctgccg
      301 tcggccgatg aacccgacga taaaaatcgg caatattgta aaccttgtgt gcacggattt
      361 catgtatctg tttgagaatt tcaggcacgt tttcctgctt ggccatcctg ggataggcgg
      421 cggcaaagac ttcatatttc tttttcgcgt ccatcagcgg cgtatcatcc cagcttacgc
      481 ctttatagct gttgacgtaa gccttcgccg tctgcaacat tgcataggct cgccgcgacg
      541 aacgcggata ctgcttttca aaatcctcgt acgtattgat ggcgtccttg taatcgcgat
      601 tgttgaaatg gtagtcggca actctcagca gacttcgctg gccgagttcc gagttcggcg
      661 aatgcgacga aattcgcaac aggatatcta tgccctcgtc cttggctgag agtttgagta
      721 tcttaagcgc gcgacgtttt cggccgttaa gaaatgcctc ggcgattttg tattcgcgat
      781 caagtgcgcg gtcgaagaat gcgccgttgg gatatgcggc tacctggtct tcaaatcgct
      841 ggtaggcggc ccagtattcg ccctgatcca tacatgcctg gccggccaga ttcatggctt
      901 cctcacatgc gggtgtgccg gcgtaattca caagaaaaac gtcggcttcc tttatggcgg
      961 ttttataacg cccttcttcg ataagttcgc gaatgtagtt tatatcgccc tgcggcgtgc
     1021 cgcgagccgg cggcttttgc gacacccagt ttttcccgtt ccagaccatc gtctcgccgt
     1081 ccgcgcacac ggacacacag gcaagcgaca tggtcaccaa cattattata atcagtaccc
     1141 ctatggcagg ggtggcggcg tcgttttgtc ttttttgaaa gctattcatt catatccgcc
     1201 aacagcctgg ctggttggac acggaaacag cgcagttaca gaagattata cgtttcaggg
     1261 ctgatgatgc aagataatac atgcctgtcg gagtgatatg aaatcattgt ttttcctggc
     1321 ccaggcgcat gaatccgccg gatttccgca aaattgacgt agatacccca aaataaacca
     1381 taggaagcca taaaaaaagc cggcttgacg aggagacagc aaaccggcct tgtgaaatgg
     1441 aaaaataata tgttttatag cgattcctga aaataaccgc aaatgtttcg gcatcatctg
     1501 caaagacaaa accatcattt aagagcggtt ctcaaaatta ttttcctttt gaagcttgct
     1561 gcaagtttcc ctgctattgt ccgtctgcat cggaaaccat gcaggttgcc tgcttcgacg
     1621 tccttagcag gaaaactttc gctgacgctc aaaaagaaaa ttctttagag aaccactcta
     1681 agctgtcgtt actgcatatt acggcattaa catttctata agctgcgttt atgatttttc
     1741 tttttctttt ttcttctgaa tatcggcggc tttgactatc caggaacaga tgccttttgc
     1801 aaggccctga gccttctcct tgacgccctg attgaccgat tcctggctgc ggcctacgca
     1861 catggcctgg cccagaattc tgccgctagc cttgtcaacc agcgtaactt cggcaaccac
     1921 ttcctccaga gggccgaaaa gctggccgga tgcgccggct ttttcataat agattattct
     1981 gccgttaatt acgactgttt catcagccgc gccggtcgca ggtatattat cctccgccat
     2041 ctgcctggcc atcgcggcag gaagctctct caaaagctca ccaggaatga ttcgcttgtt
     2101 gacatcctca aatttgccca gctggaaatt tcgcccagcc agccggccgg gattatccaa
     2161 ctcctgatag ccgcctttgg cgccggtggc tgttcctacg ccttctttta ttattcggct
     2221 gacgcagccg ctcgaaatca gaagcgtcac aaggccaatg ccggcaagca gcacttttgc
     2281 agatttcatt ttccgtccct ttcaaaaaat cattcccaac ttcgccggct gtcccgaatc
     2341 gccagccaac gacttaattc taggtttcat ccggaataat atcgacaaaa aaacaaaaaa
     2401 aaggataacc accgccggtc atcctttttc tgctacacct taaagcatgt taaatttgac
     2461 atgctcaaaa atcagcttca atgccgcggg ccgccgggcg ggcctggagg agtaatgccg
     2521 cgtgctttca tcctggccat catgtcgctc ataaactgat gcatctgtgc tcgctcctgc
     2581 ggcgaggatt tttccatatg ttcacgcata tgcgattcac gcgatttgcc gtcgtcaggc
     2641 tgactttcgg gctttttatc agtatcttcc ttacgacgtt tgtccatttc cgcacgatgc
     2701 ttttccatgt cagctatcat ttcatcgagc atttcatttt tctcttccgc cgttttcagg
     2761 gcaaaatatc tattcatccg atcctgcatc tgctgccgga acatattgcc catgttgcga
     2821 cgaagattct cccgttcctt ttcagtcagg ctctggcgtt gtgaaaacat ggcgtcgcgc
     2881 atttcaggat tttcacgcat ctggtccaaa aattctttct gcttgacgcc gtcgagtttg
     2941 ttaaaattgt cgctggcaat gaatttgaca acattcgtgg catttgccgg cgtcacttcg
     3001 ggagattggg aatcggacct gaacagtgca aacgctccga caaccacagc tataaggact
     3061 attccgccga cggtcatcaa gctaattgct ttctgttttc tggtaagctg aatcatcata
     3121 tttattagcc tcacaaaata gaagctgtct caaaacatca tcaacaggtt tgaaaccact
     3181 tccaataaaa aacacacaaa actatctatg cctgcgccac ggcagaggta ctcccaatgc
     3241 cttagcccgc gcaatgaggt ctgcgtgaaa ctgccttcgc tgagcatgct gctgaggcga
     3301 cgcggtttct ctaaaacgca tgcgacgctg ttgacgggaa gcattgtcgt ttgcctgagg
     3361 tctgttgtta ttggccggcg tcgcatttgg ctgttgcggc ctggcttgac tgtgcttctg
     3421 catatccgcc agaatttcgt ccagtatttt atttttttct tccggcgttt tcatcgaaaa
     3481 atatttatcc atccgctcct gcatctgctg ctgacgtatc gtacgcatat tctgccgcat
     3541 attgctgcgt tcatcgtcac tgagattttc ccgctgctca gcaatcgcca ggcgaatctg
     3601 cgaatcctca ttaatctggc ggacatattc cgttttcgca tcggagtcca gtttggcgaa
     3661 ttgcgggctg gcgatgaatt tcgccacggc cggcgcattc tgaacggtag ccgccggcgg
     3721 cacatctgtt cggatacatg caagtaaaat tgctgctccc agaagcagaa ccaatccgcc
     3781 ggcagctgac gacaggacga ttatcttctg ccgcttcgtg aggctaattg gccttattcc
     3841 cgacagcttt tgcaatatgt gattaacaat aatcaattct ttctcttttt attagaacct
     3901 atcccaaaaa ccttggctgc ctgcaatcgg ctcttttgca gc
//
LOCUS       DAONFI010000216         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_27843_length_3937_cov_4.276919, whole genome shotgun sequence.
ACCESSION   DAONFI010000216 DAONFI010000000
VERSION     DAONFI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27843_length_3937_cov_4.276919"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1568)
                     /locus_tag="PLK08_07350"
     CDS             complement(231..1568)
                     /locus_tag="PLK08_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPS53350.1"
                     /translation="MNNSIKAGFAKTDITPAMGIRLGGYGLAKRPAESVHDNLYSSAL
                     VLEQNGVRCVLISLDWLCITSDVAANIKNAVHEKTGTPLDNIIVCAIHTHTAPNTMAI
                     PGWGDVEYGYVNAVLPAIIDSAVQAAGKLSPAKLGVAAIESKVGVNRRTMRADGKIYF
                     DGNVDGSYDPTMTVIRFVDMQDAPITTVIHYSAHCTAWGMPRIVSRDWAGIMLDRVQK
                     QTGSPAMFVNGAIGDVGPRTNQVAGEGRFSAGIGDGLESVLEVGYRGATDALNCFFGI
                     NTFDESPILKAAVSDIEVPVKPLPPLDEVNAKLDELKEFKDTWGDKGDKKCQYEYYRR
                     VSEAYSQPPHKSNIIESRIIAIGPVAFITLPGEPFSSISLRLREGSPYPYTLLLSNAN
                     GSIAYIPDLDAREKGGYEVEMESAVNTYLPIENSDEIIVAEGIRKLRQLYNMK"
     gene            complement(1565..2821)
                     /locus_tag="PLK08_07355"
     CDS             complement(1565..2821)
                     /locus_tag="PLK08_07355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPS53351.1"
                     /translation="MTKTTDSKLAILGGEALCSKPVNAIPWPPVDDETAEQLKQVYLS
                     RNWSFGGPEEMKFNQEFAKFHDAEYAISMANGTVTLECALHALCVGRGDEVIVPAYTW
                     MATAMAARYVGAQIVFVDVEPDTLCMNPKKFEAAITDKTKAVIPVHILGSMADMEAIL
                     AIAKKHNIYVIEDCAHMQGGKWNGKGVGSWGHVGSFSFQQSKTLASGEGGICITNDAD
                     LADKIARIKHIGYPVGAKQGAAMTGPQAGLICHNYRSTEFQAVILRGQLKHLPELLEK
                     YSSNAKYLAELTKDIPGIRLQKPGRLATRQGYYGLAIMFDEGELAKIDKEIILKAINK
                     EGIACLYGVYGPVYKHILFNLPQSDWRIAGDCSVTDITVDKHTLSFPHQALGVSRNDI
                     EIIADVLRKVANNAGELIKYQSENQL"
     gene            3558..3884
                     /locus_tag="PLK08_07360"
     CDS             3558..3884
                     /locus_tag="PLK08_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPS53352.1"
                     /translation="MIFNRKAFRRFRIEKEMSQVEVARLAGCLGNDISRYERGEKIPR
                     EGRIRRLAQALSVPESTLYTNPPTQSDAIRARIEMLLEDMSEAEKAEILTIMLRMAEK
                     RQKPTE"
BASE COUNT         1071 a          928 c          866 g         1072 t
ORIGIN      
        1 cgaagccgag ctttttgtac agttcgagtt ttttgttgaa cggaatggaa tcccgcactg
       61 acggcccgaa cgggtctgcg ccttcgtgaa tgttccacgg cccaaatgaa aatacgtatc
      121 ctgaattatc cataatcatc tccattaatt atcaaacctt aatatacacg actatactga
      181 atgtagctat gaacacggct gtcggaaaca tgaacaatcc tgcactgatt ttatttcatg
      241 ttataaagct gtctgagttt tctaatgcct tccgcgacta tgatttcatc gctgttttct
      301 atcggaagat acgtgttgac ggctgactcc atttcgactt cataaccgcc tttttcacgt
      361 gcatccaaat ccggaatata cgctatgctg ccgttggcgt tactgagaag caacgtgtat
      421 ggataagggc tgccttctct gagccgcagt gaaatcgacg aaaatggttc gccgggaagt
      481 gtaatgaatg caaccggccc tatagctatg attcgtgatt cgatgatgtt gcttttgtgc
      541 ggcggctgcg aataggcttc agaaacgcgc cgataatatt catattgaca ctttttatcg
      601 cccttgtcgc cccatgtgtc tttaaattct ttgagttcat caagtttagc gtttacttca
      661 tcaagcggcg gaagcggttt gaccggcact tcaatatcac ttacggcggc tttcaatatg
      721 ggcgattcgt caaatgtgtt gatgccaaaa aaacagttca atgcgtcggt cgcaccacga
      781 tagccgactt ccagtactga ttccagcccg tcaccgatgc cggcggaaaa tctgccttcg
      841 cctgcgacct gattggttcg tggcccgacg tcgcctattg caccgtttac gaacatcgcc
      901 ggcgagccgg tctgcttctg aacgcggtct aacattatgc cggcccagtc gcgagaaact
      961 atccgcggca ttccccatgc ggtgcaatgt gcactataat gaattaccgt agtaatcggg
     1021 gcatcctgca tatcgacaaa acggattacg gtcatcgtcg ggtcatatga gccgtcaaca
     1081 ttgccatcaa aatatatttt tccgtcggca cgcatggttc tgcgatttac tccgacttta
     1141 ctttctatcg cggctacgcc aagtttagcc ggcgaaagtt tcccggcagc ctgaaccgcc
     1201 gaatcgataa tagccggcag tactgcgtta acgtaaccat attccacatc accccaacct
     1261 ggaatcgcca tcgtatttgg tgcggtatgc gtgtgaatag cgcagacgat gatgttatcc
     1321 agcggcgtgc cggtcttttc gtgcaccgcg ttcttgatat ttgcggcaac atccgaagtt
     1381 atgcataacc agtcaagcga tatgagtacg caacgcacac cattctgttc cagcaccaat
     1441 gccgacgaat acaggttgtc gtgtaccgac tcagccggcc gtttcgccaa gccgtaaccg
     1501 cccaggcgga tacccatcgc tggagtgata tcagtttttg caaaaccagc tttaatcgaa
     1561 ttattcataa ttgattctcc gactggtatt ttatcaattc gccggcattg ttggcaactt
     1621 ttcgcagcac gtcagctatt atttcgatgt catttcgtga aacgcccaat gcttgatgcg
     1681 gaaaacttaa cgtatgttta tccaccgtaa tatccgtaac gctgcaatcg ccggcgattc
     1741 gccaatcact ttgcggcaga ttaaacagga tgtgtttata aaccggcccg taaacgccgt
     1801 ataaacacgc gatgccttcc ttatttatag ccttcagaat gatttccttg tctatttttg
     1861 ccagttcgcc ctcatcgaac attatcgcca gtccgtaata accctgccgc gtggcaagtc
     1921 tgcctggctt ctgcagccga atgcctggaa tatcttttgt caactcggcc agatactttg
     1981 cgttggaact atatttttcc aaaagttccg gcagatgctt caattggccg cgaagaatga
     2041 cagcctgaaa ttcggtagaa cgataattgt ggcagatgag tccggcctgt gggccggtca
     2101 tggcggcacc ctgctttgca ccaaccggat aacctatgtg ctttatgcgg gctatcttgt
     2161 cggcaaggtc ggcatcattg gttatgcata tgccgccttc gccgctggcg agcgttttgg
     2221 actgttgaaa gctgaaactg cccacatgcc cccaactgcc tacgcctttg ccgttccatt
     2281 ttccgccctg catatgggca cagtcttcta ttacgtatat attgtgtttt ttcgctatgg
     2341 caagaatcgc ttccatatcc gccattgagc ctaaaatatg tacgggaatg acggcttttg
     2401 tcttgtcggt aatggcggct tcgaattttt tgggattcat gcacagcgta tcaggctcga
     2461 cgtctacgaa cacgatttgg gctccgacgt acctggcagc catagctgtc gccatccagg
     2521 tatacgccgg cacgataact tcatcgcctc tgcctacgca caaagcatga agtgcacatt
     2581 caagtgtcac agtgccgtta gccatggaaa tggcatattc ggcatcatgg aattttgcaa
     2641 actcctggtt aaacttcatc tcttcagggc cgccgaacga ccagttgcgg ctgagatata
     2701 cctgtttgag ctgttccgcc gtctcatcat cgactggcgg ccacggaatt gcattgactg
     2761 gtttactgca aagagcttct ccgccaagaa tagccaattt gctgtcggtc gtttttgtca
     2821 ttttaaatac tcctgtaaaa catcgttgtt ctgataattc aatcataccg acagataatg
     2881 attgcaaaat ggaatattca gaaatgatat ggaaaaatcg gtacatattg gaataggaat
     2941 tgtcacaata gaaggacagg taatactgaa cgactatttg cttagaatgg ttctggaaaa
     3001 gaatattcat tgagcaaact tttgttaaaa aaccgtagaa ttacgaatgg cctattaaac
     3061 gcaacgtcga agcgagattg actgaatggc cggcaatgac gtttaagcag acaaccctgt
     3121 tttgatacag atggacgctt aataaaattc aggcggtgaa agtttgcgag gagtgaaaac
     3181 acagacagaa ccagccgcct gaacctactg ctataccgtc aaattaactc gtattcagta
     3241 ccccgtatta aggaatacac cctatgtaaa aacacactta acaaaacaat aagtttgagg
     3301 aacatatgca aaaggcatta cggaaaacac aggacttcca cgattctaaa cagatgaaaa
     3361 aacgtattta tttaaaaaaa acccggctct gacaaatctt acatgtgttt aatatatgca
     3421 ttcatattca ttatttaaag caaaaatatc cataaatctt cactttgaat ggctttttat
     3481 gactaaatat aagaagaaca aagagttaca aagtcaaata aatttactga aaatgaaatt
     3541 atggtatctt ttatattatg atatttaaca gaaaagcatt tcgaagattc agaatagaga
     3601 aggaaatgag ccaggttgaa gtagctcggc ttgccgggtg ccttggaaat gacatttccc
     3661 gttatgagcg aggggagaaa ataccgcgtg aaggccgaat cagacgtctt gcccaagcat
     3721 taagcgtgcc tgagtctaca ctttatacca atccccctac gcaatctgac gccattcgtg
     3781 cgagaattga aatgttgttg gaagatatga gtgaagccga aaaggctgaa attctaacga
     3841 ttatgctccg aatggctgaa aaacgtcaaa agccgacaga gtaaaatcag tcgaaaaaat
     3901 agcaaattga aacctgataa ggctagccta ttagggg
//
LOCUS       DAONFI010000217         3924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_27971_length_3924_cov_4.408633, whole genome shotgun sequence.
ACCESSION   DAONFI010000217 DAONFI010000000
VERSION     DAONFI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27971_length_3924_cov_4.408633"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLK08_07365"
     CDS             <1..350
                     /locus_tag="PLK08_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HPS53353.1"
                     /translation="RRMGLPTWDKPAMACLASRVQYGNEITAEKLTNIEKAENFLKDL
                     GFSHVRVRHHGTLARIEVPKADFQQLLSNAAIITEYITKSGFVYVTFDLAGFRSGSSN
                     EVLFAGEATEENR"
     gene            350..1141
                     /locus_tag="PLK08_07370"
     CDS             350..1141
                     /locus_tag="PLK08_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPS53354.1"
                     /translation="MIINSSSIAERYSRQIAFDGWSDARQQLLGQSRVLIVGVGGLGS
                     WVAELLVRAGVGFLRLADYDTVEISNIHRQAMYDELDAQSGMYKADVAGNHLRQINGK
                     CKIEPACEKLDRFNIFDLTDGIDLIIDGTDNFWTRFIINDYCVKNGMPWIFGGAVRAE
                     GQMAVIIPGKTLCLRCLLDYPPAQCDDSSCAQSGVFGPAVAAVAAFQAMEAIKYLGGC
                     DEMLNPHVMKLDMWSNRVQYFDATSHEKIEPCPCCDLHEFEFLEP"
     gene            1156..2322
                     /locus_tag="PLK08_07375"
     CDS             1156..2322
                     /locus_tag="PLK08_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPS53355.1"
                     /translation="MNQAEKITYLDNAATTQIDPTVLARMVQVYTEDFGNSSNTTHGY
                     GKRASELVELSRAEVALSLGALPREVIFTSGATESCNSAIKGVAELYSPRGRHIVLSQ
                     IEHSAVIEPCRRLERMGFELTWLRPDENGVIDQSQVADAIRRDTILVCVMAANNVTGV
                     INPVADIASICKRRGVPYFCDATQAFGKIPLAFEEMGIDLMAVSSHKIYGPKGVGALV
                     VRSRSPRVRISPLHDGGGQERGLRSGTLNTPGIVGFAKSCEIISQNMIEESAKISAMR
                     NMFERIVTSKIPQIKILSHMVTRLPGISNIIFDTFDAREILKSIPQIAASAGAACGTD
                     AISPNHVLLAMGLTKDAAAASVRFSFGRFNTEDEVRFAAELLAESFNRRFRQKV"
     gene            2324..3427
                     /gene="nadA"
                     /locus_tag="PLK08_07380"
     CDS             2324..3427
                     /gene="nadA"
                     /locus_tag="PLK08_07380"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPS53356.1"
                     /translation="MTTIEQSYYEIDSEELVARICSHKAALGQRLCIMAHHYQCDDTV
                     QFADFVGDSLYLSQKAAEQKEAEFIVFCGVRFMAESADILSSGRQRVCLPNMYAGCAM
                     SDMAAAGDVEVALCEIKSLLHGGQRIVPVSYVNSSAEIKALTFDWGGACCTSANVKRV
                     FEWALTPLSQGGGGADKILGLPDRHLMLNTIVSMGYTADDCAVYDPVLPDGGFTPEKL
                     DSVKFILWPGHCYVHDNFSLSDVARVCRDYKDARIIVHPECRYEVVMAADKVCSTTQI
                     IRYVDAEKPGTHIAIGTEINLVKRLARQHPDKNIVCLAESGTRCVQMAKIDLPHLLWS
                     LDSIAACSPVNVISVPDGIALKARDVLERMINL"
     gene            complement(3478..>3924)
                     /locus_tag="PLK08_07385"
     CDS             complement(3478..>3924)
                     /locus_tag="PLK08_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPS53357.1"
                     /translation="KWNTQNFYNFIRNPIYYGLERWRYKKVLKSETTGHSRPVRAEDD
                     YVMYRDMPHLAHVSKALWDKANAVIANRCTHNNKKTGRDNPRFGVSLKNDPTRLGPKL
                     KCWICGGTLRRRGPHIDGIYSPALPAVPDIHILTDHLGIKAHLSTQ"
BASE COUNT         1019 a          826 c         1067 g         1012 t
ORIGIN      
        1 caagacgaat gggcctgccg acgtgggaca aaccggcaat ggcgtgcctg gccagccgcg
       61 tacagtatgg caacgaaata acggctgaaa aacttacaaa tatagaaaag gctgaaaatt
      121 ttttgaaaga ccttggcttc agtcatgtgc gggttcgaca tcacggaacg cttgcgagaa
      181 ttgaagtacc caaagccgat tttcaacagc tattgtccaa tgccgcaata attacggaat
      241 atattacaaa aagcggattt gtttatgtca cgtttgacct tgccggtttt cgcagcgggt
      301 catcgaatga agttttattt gccggcgaag cgacggagga aaatcgttaa tgattatcaa
      361 tagttcgtca attgcagaga gatattcgcg tcaaattgcg tttgacggct ggagcgacgc
      421 gagacagcag ttactgggcc aaagtcgcgt gctgatagtc ggagttggcg gactcggttc
      481 atgggttgcg gagcttcttg tgcgtgctgg tgttggcttt ttgcggcttg ctgactatga
      541 cacggttgag ataagcaata tacaccggca ggcgatgtat gatgagttgg acgcgcagtc
      601 aggcatgtat aaagctgatg ttgccggaaa tcatctgcgt cagattaacg gaaaatgcaa
      661 aattgaaccc gcgtgcgaaa aactggacag attcaacata ttcgatttaa ctgatgggat
      721 agacctgatt attgacggga cggataattt ctggacgcgg ttcataataa atgattattg
      781 cgtgaaaaac ggcatgccct ggatttttgg cggggctgtg cgtgctgagg gacagatggc
      841 ggtcataatt ccaggaaaga cgctttgtct gcggtgtctg ctggattatc cgcctgcaca
      901 atgcgatgac tccagttgtg cgcagagcgg agtattcggc cctgcggtgg cggcagtagc
      961 agcttttcag gcgatggagg cgataaaata tctcggcggc tgcgatgaaa tgctcaaccc
     1021 tcatgttatg aagctggaca tgtggagcaa tcgggtccag tatttcgatg cgacttcgca
     1081 tgaaaaaatt gaaccatgcc catgttgcga cttgcacgag tttgaatttc ttgagccatg
     1141 acaaaaggtg tacgaatgaa tcaggcagaa aaaatcacat atcttgataa tgccgcaact
     1201 acgcagattg acccgacagt gctggcacgc atggtgcaag tttatactga ggattttggc
     1261 aattcttcaa atactacgca tggttacgga aaacgtgcga gcgaacttgt ggaactttca
     1321 cgcgcggaag ttgcgttgtc gcttggggcg ctaccgcgcg aagtaatatt cacatcaggc
     1381 gcaacggaga gctgcaattc tgccataaaa ggcgttgcag aattatattc gccacgtggc
     1441 agacacatag tgctttcaca gattgagcat agtgcagtca tagaaccatg caggcggctt
     1501 gaacggatgg gatttgagtt gacgtggttg cggcctgacg agaatggggt catagaccag
     1561 tcgcaggttg cggatgcaat ccgtagagac acgattctgg tctgtgtgat ggcggcgaat
     1621 aatgttacag gcgtgataaa tcctgtggct gatatcgcgt caatctgcaa gcgtcgtggt
     1681 gtgccgtatt tttgtgatgc tacacaggcg tttggaaaaa ttccgctcgc attcgaagaa
     1741 atgggtattg atttaatggc ggtttcatcg cataaaattt acggtccaaa aggtgttgga
     1801 gcacttgtag ttcgcagccg ttcgccacga gtgcgtattt cgccattaca tgatggcggc
     1861 ggtcaggaac gtggcctgcg cagcggaaca ctaaacacgc ctggcatagt cggattcgcc
     1921 aaatcatgcg aaataatatc gcagaacatg attgaagaat cagcgaaaat ttctgccatg
     1981 cggaatatgt ttgaacggat agtcacttca aaaattccgc agatcaaaat tttgagccac
     2041 atggtcacga ggctgccggg cataagcaac attattttcg atactttcga tgcacgcgaa
     2101 attttgaaat cgattcctca aattgctgca tcggctgggg ctgcgtgcgg aacggacgca
     2161 ataagtccaa atcacgttct gctggcgatg ggtcttacca aagatgcggc agccgcttct
     2221 gtgcggttca gctttggccg attcaacacg gaagatgaag tacgttttgc ggctgagttg
     2281 ttggcggaat cattcaacag acgtttcagg cagaaggttt gatatgacaa ctattgaaca
     2341 atcatattat gaaattgatt cagaggaact agttgctcga atatgctcgc acaaggctgc
     2401 gcttggccaa cggctgtgca taatggcaca tcattaccag tgcgacgata cggtacagtt
     2461 tgccgatttt gtaggcgaca gtctgtatct ttctcaaaag gcggcggagc agaaagaggc
     2521 tgagttcata gtgttttgcg gcgtgcggtt tatggcagag tccgctgata tactttcctc
     2581 cggcaggcaa agggtatgtt tgccgaatat gtatgcaggt tgtgcaatgt cagatatggc
     2641 cgctgccggt gatgttgaag tcgctctatg tgaaatcaaa agtttattgc acggtggaca
     2701 acgaattgta ccggtgtcat atgtgaattc atcggcggaa atcaaagcac tgacgtttga
     2761 ctggggtggt gcgtgttgca cgagcgctaa cgtcaaacgt gtattcgagt gggcactgac
     2821 tccactatcg caaggtggtg gtggcgcgga caaaattctg ggtttgccgg atagacatct
     2881 tatgctcaat acgatcgtgt cgatgggata cacagccgat gactgtgcgg tgtatgaccc
     2941 tgtacttcca gatggaggat ttactcccga aaaacttgat agcgtcaaat tcattctctg
     3001 gccaggccat tgctatgtgc acgataattt ttcattgagt gatgtcgctc gggtatgccg
     3061 agattacaag gacgcgcgaa ttatcgttca cccggaatgc cggtatgagg ttgtgatggc
     3121 agccgacaag gtctgcagca caactcagat tatccgttac gtagatgccg agaaacccgg
     3181 tacgcacata gcaatcggaa cggagataaa tctcgtgaaa cggttggcac gacagcatcc
     3241 tgataaaaac atagtttgtc ttgctgaaag cgggacacga tgcgtgcaga tggcaaaaat
     3301 agatttacct catctgctct ggtcgcttga ctccattgct gcctgcagcc ctgtgaacgt
     3361 aatcagcgtg ccggatggca ttgcgttgaa ggcacgcgat gttttagagc gaatgataaa
     3421 tctgtaacgt cggtacaata tttagggtta ttgagacaat catttctggg cagagggtta
     3481 ctgtgttgac aggtgggcct tgatgccgag atgatccgta agtatgtgaa tatcaggaac
     3541 ggcaggtaaa gcaggcgaat atatcccgtc aatatgcggg cctcttctgc gaagagtacc
     3601 accgcatatc caacatttga gtttgggccc gagcctggtt gggtcatttt tcaaactgac
     3661 cccgaagcgt ggattatccc gacctgtctt cttattgttg tgcgtacacc tattggcaat
     3721 cacagcgtta gccttgtccc ataatgcctt gctgacatgt gccaggtgcg gcatatcacg
     3781 atacattaca taatcatcct cagctcgaac agggcgactg tggccagtcg tttcagattt
     3841 caaaaccttc ttatatcgcc atcgctcaag accataataa attggattgc gaataaaatt
     3901 gtaaaaattc tgcgtgttcc attt
//
LOCUS       DAONFI010000218         3916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28048_length_3916_cov_3.933696, whole genome shotgun sequence.
ACCESSION   DAONFI010000218 DAONFI010000000
VERSION     DAONFI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28048_length_3916_cov_3.933696"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..713
                     /locus_tag="PLK08_07390"
     CDS             48..713
                     /locus_tag="PLK08_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53358.1"
                     /translation="MTASKKIKFGRSFFISLLIHVILIAVLATVAVHIYEKHRGSADN
                     GQLQAIAARPDINIDTVKTSLQKEEARVAELSDEDKMSELRKKSAVLQNMNPQAVDAV
                     AAAAETLGRANKSRAYEPVKNVAGNFDTDTAAIYDISRENRDEQPVFIYTLVDAKGRT
                     MKAERRMDEMSADDMRAYHIFEMSRGNKNMARLLRSAIMIGQPQADDATRQSPAAAAE
                     TAK"
     gene            721..1443
                     /locus_tag="PLK08_07395"
     CDS             721..1443
                     /locus_tag="PLK08_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPS53359.1"
                     /translation="MKKETLMRHFTEWLKHNRTHKQNYPFVFMLSMFALMLFFPVFTK
                     TNTAKLALCTMFTLVIITSFLSVRRNRIFIASVLILAVPLLILAWLDCIANIEHPASD
                     IAQAIMLCFFFVLLFSITVYKIVSESHAVTKHTIFNAISAYLLLGLFFAGIYAVLALC
                     GVKVFSIESTQNTTNLHDVWDISAYFSYCSLTTLGLGDVTPASPVSRSLTTIESVIGP
                     LYLSILIARLVSTYKNSHKNQD"
     gene            1552..2595
                     /gene="cheB"
                     /locus_tag="PLK08_07400"
     CDS             1552..2595
                     /gene="cheB"
                     /locus_tag="PLK08_07400"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HPS53360.1"
                     /translation="MIRILIVDDSTINRAMLRHVFEQQPDMVVVGEAVNGLQAIEMAE
                     TCSPDIITMDIFMPVLNGFDATRNIMSQKPIPIVILSASIEPGEVELCFHALEAGAVT
                     AQQLPSTKDQASIDNLLSIIRTMSSITVHSIPTEPSPNYVFKTFSRSTTPVVAIGSST
                     GGPPILQKILSRMPEDYSFPIAIVQHIAKGFTTGLVEWLNASSQIEVKMAVNDEQMRP
                     GTAYLAPDDYHMKVSPERTIVLSQEPPQNDIRPAVSKLFESLAKNCCPTAIGVILTGI
                     GSDGAKELKMMKDAGAINIAQDIESSIVFDMPRAAIQLGAVNHILPPCRIASQLIAMA
                     KQYQTSSESVFSN"
     gene            complement(2607..3782)
                     /locus_tag="PLK08_07405"
     CDS             complement(2607..3782)
                     /locus_tag="PLK08_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPS53361.1"
                     /translation="MRRIYMDNSATSFPKPSSVLQTMIDFAENCGASAGRGAYAEAKH
                     CEKILATCRARIAKLINAESPQTIVFTLNCSDGLNIAIRGVLNTAPAGSHAITTALDH
                     NSVLRPFNALAEQTGLQPQFIECDSVTGLVDPDDIKKALRPSTRLIACVHVSNVTGTM
                     QPIDDVVKIAREAGVTSIVDAAQSIGHTPIDVQSLGADFVAFPGHKGLLGPLGTGVLY
                     VKPGSEKNCPTMKEGGTGTVSELATHPQTMPDKYEVGSHNAIGIAGLDAGVAWLLEKG
                     VDKLRAHDRRLCEVFLKATDGVKNLKVYGPPLTHLDCRAGVFSVNAGDMTPHELADEL
                     EKKYGILTRPGVHCAPLAHKTIGTHPVGTCRLSFGAFTTEEDVIFAAESLSKIANSL"
BASE COUNT         1015 a         1062 c          953 g          886 t
ORIGIN      
        1 ggctaagagt tcgacgcata aacaccgccg agaaagatga cgaataagtg accgcatcca
       61 aaaaaatcaa attcggcaga agttttttca tatcgctgct gattcacgtc atcctgatag
      121 cagtcctggc gactgtcgcc gtacatatct atgaaaaaca tcgcggctcg gcagataacg
      181 gccagttgca agctatcgcc gccaggccgg atataaatat cgacaccgtc aaaaccagcc
      241 ttcaaaaaga agaagcccgc gtagctgaac tgtccgacga agataaaatg tctgaacttc
      301 gcaaaaaatc ggcggttctg caaaatatga acccgcaggc tgtcgatgca gtagctgcag
      361 cggcagaaac gctcggcagg gcaaacaaat caagagccta cgaacccgtc aaaaacgtcg
      421 ctggcaactt cgataccgac acggctgcga tatacgacat cagccgcgaa aatcgcgacg
      481 aacagccggt tttcatatat actctcgtcg atgctaaagg cagaaccatg aaagcggaac
      541 ggcgaatgga tgaaatgtcg gctgacgaca tgagagcata ccatattttt gaaatgagca
      601 gaggcaacaa aaatatggca aggctgctca ggtcggctat aatgataggc cagccgcagg
      661 ctgacgacgc aacgcggcaa tcccctgccg ccgctgccga aaccgctaaa tgaaattctc
      721 atgaagaagg aaacccttat gagacacttc acggaatggc tgaaacacaa caggacgcat
      781 aaacagaatt atccgttcgt tttcatgctt tccatgtttg cactgatgct gttctttcca
      841 gtattcacca agaccaatac cgcaaaactg gctctctgca ccatgttcac actggtaatt
      901 atcacgtctt ttctgagcgt gcgacggaat cgcatattca tcgcatccgt actgatactg
      961 gctgtgccgc tgctgattct ggcgtggctg gactgcatag caaatatcga acaccctgcg
     1021 tcagacatag cgcaggcgat aatgctctgt ttctttttcg tcctgctttt ctccataacc
     1081 gtttataaaa tcgtctcgga atcgcatgcc gtcacaaagc ataccatatt taacgccata
     1141 tcggcgtatt tgctgctcgg cctgtttttc gccggcattt acgcagtgct ggcactgtgc
     1201 ggtgtaaaag tgttttccat agagtcgacc cagaatacca ccaatctgca tgacgtctgg
     1261 gatataagcg catatttcag ctactgttcg ctgactacgc tggggctggg agatgtaaca
     1321 cctgcatctc ccgtcagccg ttcccttaca accatagaat ccgtaatagg gccgctgtat
     1381 ctttcaatcc tgatagcgcg gctggtaagt acatacaaaa attcccacaa aaatcaagat
     1441 tgattgtaaa cgggattttt ctatcctatg atgtaattta aaagagagat gaatgtgacg
     1501 ccaggaggct gatgtctgca acatcaaatt aaacgggagt ggacgcctgc catgattcgc
     1561 atattaatcg tagacgacag caccataaac cgcgcgatgc tcaggcatgt tttcgagcag
     1621 cagccggaca tggtcgtagt cggggaggcc gtcaacggct tgcaggctat cgaaatggcc
     1681 gaaacatgca gtcccgatat aatcaccatg gatattttca tgccggtact caacggcttt
     1741 gatgccacac gtaacatcat gtcgcaaaaa cccattccga ttgtgatact ttctgcaagc
     1801 atagaacccg gcgaagtaga actgtgtttt catgcgcttg aagccggcgc cgtcacagcc
     1861 cagcaactgc cgtccacaaa agaccaggcc tccatcgaca acctgctgag catcatacgc
     1921 acaatgtcgt caataactgt acattccata ccaaccgagc cgtcgcctaa ttacgtgttc
     1981 aaaacgtttt cacgttcgac gacgccggta gtcgcaatag gctcatctac aggcggcccg
     2041 cccatacttc aaaaaatact ttcgcgaatg ccggaagact atagttttcc catagcgata
     2101 gttcaacata tcgcaaaagg ttttacgaca ggccttgtgg aatggctgaa cgcctcgtcg
     2161 caaatagaag taaaaatggc ggtcaacgac gaacagatgc ggcctggaac ggcgtatctt
     2221 gcgccggacg attatcacat gaaagtctcg cccgagcgga ccatcgtcct atcgcaggag
     2281 ccgccgcaga acgacatacg gccggcagtg tccaagctgt ttgaatcgct ggcgaaaaac
     2341 tgctgcccga ctgctatcgg ggtgatactc accggcatcg gcagcgacgg cgcaaaagaa
     2401 ctcaaaatga tgaaagacgc cggagcaatc aacatcgccc aggatataga aagttcaatc
     2461 gtgttcgata tgcccagagc cgcaatccag ctcggcgccg tcaaccacat cctgccgccg
     2521 tgccgaattg cttcgcaact gatagccatg gccaagcaat accagacttc atctgaatca
     2581 gttttttcaa attagtgaga accgctctaa aggctattcg cgattttcga caacgattcc
     2641 gctgcgaaga ttacgtcttc ttccgtggta aatgcgccga aactcagacg gcatgtgccg
     2701 accggatgcg tgccgatggt cttgtgcgcc agcggcgcgc aatgtacgcc cggccgcgta
     2761 agtatgccgt attttttctc cagttcatca gccagttcgt gcggcgtcat gtcgccggcg
     2821 ttgacgctga atacgcctgc gcggcagtcc aggtgcgtca atggcgggcc gtaaaccttg
     2881 agatttttca caccgtcggt cgccttcaaa aacacctcgc acagccggcg gtcatgtgcc
     2941 cgcagtttat ccacaccctt ctccagcagc cacgccacac ctgcgtcaag gcctgcaatg
     3001 ccgatggcat tatgcgagcc gacttcatat ttatcgggca ttgtctgagg atgcgtcgca
     3061 agttcgctga ccgtgcccgt tcctccctct ttcattgtgg gacagttttt ttcactgccg
     3121 ggtttcacgt acagcactcc cgtgccgagc ggcccaagca ggcccttgtg gccgggaaac
     3181 gccacgaaat ccgcacccag cgactgcacg tctatgggcg tgtgtccgat ggattgcgca
     3241 gcgtccacga ttgacgtaac gccggcttcc cgggcgattt tcacgacatc gtctatcggc
     3301 tgcattgtgc cggtgacgtt cgatacgtga acgcaggcta tcaacctggt cgaaggccga
     3361 agggcttttt ttatgtcgtc ggggtcaacc agtccggtga cgctatcgca ttcgataaac
     3421 tgcggctgca agccggtctg ttcagccagc gcgttaaacg gccgaagcac cgaattatgg
     3481 tcaagcgccg tagttatcgc atggctgccg gctggagcgg tattgagtac gccgcgaatc
     3541 gctatattca ggccgtcaga gcaattcagt gtgaaaacta tcgtctgcgg cgattcggcg
     3601 ttgataagtt tcgcaatgcg agcacggcac gtggcaagaa ttttttcaca atgtttggcc
     3661 tcggcgtaag cacctcggcc ggcggatgcg ccgcaatttt ccgcaaaatc aatcattgtc
     3721 tgcaatactg acgacggctt gggaaaactc gtcgcggaat tgtccatata tatacgtctc
     3781 ataatcccag ccatgatacg caatgcgcag agtttttcaa cccaaaagcc ccaaaacgcc
     3841 gataatgaca gaattacacg cgacgacgaa cgataatgtt atgtaactct ctgaaacgcc
     3901 agttaattgc gttaag
//
LOCUS       DAONFI010000219         3908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28127_length_3908_cov_4.345445, whole genome shotgun sequence.
ACCESSION   DAONFI010000219 DAONFI010000000
VERSION     DAONFI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28127_length_3908_cov_4.345445"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLK08_07410"
     CDS             complement(<1..727)
                     /locus_tag="PLK08_07410"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HPS53362.1"
                     /translation="MKIFVVNCGSSSIKYQLFSMTNEKVLAKGLVERVGTNDARIKFE
                     SADSSAEHRLDAPDHTAAMKHILEALVDPRYGVLENIDEIEAVGHRVLHGGEKIHESM
                     LIDDRVKSIIREYFQLGPLHNPANLMGIEASMKAMPGKPNVAVFDTAFFATMPPKAFR
                     YAVPNEWYERYHVRKYGFHGTSHRFVTGQASIDLGKSLDQLNIISVHLGNGCSVTATH
                     NGKAADRSMGLTPLEGLMMGTRSG"
     gene            1242..1715
                     /locus_tag="PLK08_07415"
     CDS             1242..1715
                     /locus_tag="PLK08_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53363.1"
                     /translation="MPFYTGEHETSIAAKRRMAISSALREEQTPDDGSQYYLVLGPDK
                     HLWLYPDAYYRKLASHMKRSLLPSRQAVNMSMFFAMARLLKPDSQGRVVLPEKSLERA
                     RLTDVEDVTLVGKGDHVELWPRHEWEKFVEASMDSYGEMLYEAADLFEAVDDKNE"
     gene            1773..2060
                     /locus_tag="PLK08_07420"
     CDS             1773..2060
                     /locus_tag="PLK08_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53364.1"
                     /translation="MDGGINRNGFLKTRQKWIMATLIFQRDSRAANEKGISHTDFAEL
                     RRRMENPASFDEEFRQRLEVLAERLTAVQALGDEFRRVDLSATARKLLDAR"
     gene            2081..3010
                     /gene="rsmH"
                     /locus_tag="PLK08_07425"
     CDS             2081..3010
                     /gene="rsmH"
                     /locus_tag="PLK08_07425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPS53365.1"
                     /translation="MNLKGNASEGHIAVLRNEVVNYLAPAIAKPGTILIDCTTGLGGH
                     TGTLLANAHPLSRCICIDTDARNLAQAKSRLAEYGGRLRFFHANFSDVREVLRETATG
                     KVNAVLADLGFASNQMDDPARGMSFSAEGPLDMRLDLTKGLTAEYIVNTLDENELADI
                     IYHYGEERLSRRIARAIVDARKNQPIKTTMELAQIVSSVMPPLSMLRPKGKKIHPATR
                     TFQALRIAVNDELLNLDNLLDELEEIMAPGGRVAIISFHSLEDRRVKLAFATLESAGK
                     ANILTKKPVTPADVEIESNPRCRSAKMRVIEFV"
     gene            3090..3746
                     /locus_tag="PLK08_07430"
     CDS             3090..3746
                     /locus_tag="PLK08_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPS53366.1"
                     /translation="MTRQTNIGLLLALIFIVAFSLVLTEFRQPEQNNIDRQDMASVDD
                     GYYKQPTQELVSPVRPAVRSVVRRAVRPENRRTIAAAVAPAPIQRAATAEVAVARTHA
                     AEPASQSRTAAPAQTAPAHRTEFVAGTIHMPQNNASANYREKTIDEFAQDVAQLPDRK
                     NQPTRRVYTVIRGDNLRKISKKIYNSDSKWRKIYDANRSKLKNPNDIQIGMTLVIPEV
                     "
BASE COUNT         1052 a          999 c         1020 g          837 t
ORIGIN      
        1 cgccgctgcg agtgcccatc ataaggcctt caagcggggt caggcccatc gagcgatcgg
       61 cggctttgcc gttatgggta gctgtcaccg aacagccgtt gccgagatga acagagatga
      121 tgttgagctg gtcgaggctt ttgccgagat ctatcgacgc ctggccggtc acgaaacggt
      181 gacttgtgcc gtgaaaaccg tacttgcgga cgtgatatct ttcgtaccat tcgttcggca
      241 cggcatagcg gaaggccttg ggcggcatcg tcgcaaaaaa cgccgtatcg aagaccgcca
      301 cgttgggttt gccgggcata gccttcatcg acgcctctat gcccatcagg ttcgccggat
      361 tatgcaacgg cccaagctga aaatattcgc gtatgattga cttcacgcgg tcgtctatga
      421 gcatggactc gtgaatcttt tcgccgccgt gcagaacgcg atggccgacg gcttcgattt
      481 catcaatatt ttcgagcacg ccgtaacgcg gatccaccag cgcctcaagt atgtgtttca
      541 tcgccgccgt atggtccggc gcatccagcc gatgctccgc agatgagtcc gcagattcaa
      601 actttattcg ggcatcattc gtgcccacac gctcaaccag acccttggcg agaacctttt
      661 cattcgtcat gctgaaaagc tgatacttaa tcgaactgct gccgcaatta acaacgaaaa
      721 tcttcatgac tacctcgtac aaaaatcatt ttaaaaaaac gcttccactc gcagggggct
      781 gttcaaaata aaaagcagaa cgagattgtt acaacaaacc gttcgcgttt caagtcccgt
      841 caggaaaaag aatctaaaat tttgcgatac tttaaagtat tttaaaaaat ttgaaaaaaa
      901 tttcagctct gaaacagtaa tgtccgataa ctttatatgt aagccatagc gaaaggcgtt
      961 ggcaatatga tgattgaaaa gtcggctgat tattgtggca catttaaccc gtgtttttaa
     1021 aaagagttgc actactctta ttctccggcc agccgccaca ggcataccct ctttacttta
     1081 tgcgtctgta gactgtaatt gagcggaaat gatgcgtttt cttggtgcgg cgaaatgttt
     1141 ttttgcaatt taggctaaag agaaaatttt tttcttgccg attccctatt aatggtgtag
     1201 attccctgtc agttactgat agagactaaa aggacttaag aatgccgttt tataccggag
     1261 aacatgaaac aagcatagca gccaagcgtc gcatggcgat aagctcagcc ttgcgcgagg
     1321 agcagactcc ggacgacggt tctcaatatt accttgtgtt ggggccggac aagcatctgt
     1381 ggttataccc tgatgcctac taccgcaagc tggccagcca tatgaagcgt tcactgctgc
     1441 ccagcaggca ggcggtgaac atgagcatgt tttttgcgat ggctcgcctg ctcaagcccg
     1501 acagccaggg ccgtgtggta ctgcccgaaa aatcgctcga aagagcccgg ctgaccgacg
     1561 tcgaggacgt gacgctggtc ggcaaaggcg accacgtgga actatggccg cgccacgaat
     1621 gggaaaaatt cgtggaagca tccatggatt catacggcga aatgttgtat gaagccgccg
     1681 acctcttcga ggccgtcgac gacaaaaacg aataatgaac agttcaggtt ttaaggacaa
     1741 aacctgcatg aacgacacgg atgtattccg ctatggacgg cggaataaac aggaacggct
     1801 tcctgaaaac ccggcagaaa tggattatgg cgacactcat cttccaacgt gacagcagag
     1861 ccgcgaacga aaaaggaatt tcgcatacgg actttgccga gctgcgacgt cgtatggaga
     1921 accccgcgtc attcgacgag gagttccggc agcgtcttga ggttttggcc gaacgtctga
     1981 cggcagttca ggcgttggga gatgagttcc gccgggtgga tctttcggcg acagccagaa
     2041 aattgctgga tgcccgctag cacatgacag gaggcgtcgg atgaatttaa agggcaacgc
     2101 ttccgaaggg cacatagcag ttcttcggaa tgaagttgtg aattatctgg caccggccat
     2161 cgccaagcct ggaacgatat taatcgactg caccaccggc ttgggcggac acaccggcac
     2221 cctgttggcc aacgcccacc ccctctcacg ttgcatctgc atagacaccg acgcccgcaa
     2281 tctcgcccaa gccaaatcac ggctggcgga atacggcggc agactgaggt ttttccacgc
     2341 caatttttcc gatgtcaggg aagttttgcg ggaaacggca acaggtaagg tcaatgctgt
     2401 actggcggac ctgggattcg cgtcgaacca gatggacgat ccggctcgcg gcatgagttt
     2461 cagcgcggaa ggccctctgg atatgcggct ggatctgaca aagggactca cggcggagta
     2521 tatcgtaaac acgcttgatg aaaatgaact ggcggatata atctatcact acggcgagga
     2581 acgtttaagc aggcgaatcg cccgtgcgat agttgacgca agaaaaaatc aacccatcaa
     2641 gacgacgatg gaacttgcac agattgtttc gtcggtaatg ccgccgcttt caatgctccg
     2701 gccaaagggc aaaaagattc accccgcgac cagaactttt caggcactga gaatagcggt
     2761 aaacgacgaa ctgctgaacc ttgacaacct tcttgacgaa cttgaagaaa taatggcacc
     2821 tggcggacgg gtagcgatta tttcattcca ttcactggaa gacaggcggg taaaactcgc
     2881 tttcgccaca ctcgaatcag ccggcaaagc gaatattctt acaaaaaaac ctgttacgcc
     2941 ggctgacgtg gaaatcgaat caaacccgcg atgtcgttcg gcgaagatga gagtaattga
     3001 gttcgtgtaa agtaaaaaac gtgtaaaaaa ggttcagcga cgaatctgat tttttaattt
     3061 taaaaaagga ctatcgggag gtcagggata tgaccagaca aacaaacata ggattactgc
     3121 tggcacttat atttatcgtg gcgttttcgc tagtgctaac cgagttccgg cagccggagc
     3181 agaacaatat cgacagacag gatatggctt cggtggatga cggttattat aagcagccga
     3241 cgcaggaact ggtgtcgccg gttcggccag ccgtgcgcag cgtcgtgcgc agagccgttc
     3301 ggccggaaaa ccgccggacg atagccgcag cagtagcccc cgccccgatt cagcgagccg
     3361 ccacagccga agtagccgtc gcccgcactc atgcggctga acccgcaagc cagtcacgga
     3421 ctgccgcgcc ggctcagacg gcaccggcac acagaacgga attcgtagcc ggcacgattc
     3481 acatgccgca aaacaacgct tcggcaaatt atcgtgaaaa aaccatcgat gaattcgccc
     3541 aggacgtggc acaactgccg gaccgtaaaa atcagccgac caggcgcgtc tacaccgtca
     3601 tcaggggcga caatcttcgc aaaatctcca aaaaaattta caactcggac agcaagtggc
     3661 ggaaaatata tgatgccaac cgcagcaagc tgaaaaatcc caatgacatt caaatcggca
     3721 tgacgctggt gataccggaa gtatgaggat agccaccgta attataggca taaccgccat
     3781 tgccgtgtgt cttgtgatga tgcgccgcga agacgtacga ctaagacacg agttgcagaa
     3841 aatccagacg cagcatgccg ccgtcaaacg cgatatatgg gaccgccatg tggaaatggg
     3901 tcacctcc
//
LOCUS       DAONFI010000220         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28421_length_3879_cov_3.767521, whole genome shotgun sequence.
ACCESSION   DAONFI010000220 DAONFI010000000
VERSION     DAONFI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28421_length_3879_cov_3.767521"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..813)
                     /gene="nrdR"
                     /locus_tag="PLK08_07435"
     CDS             complement(289..813)
                     /gene="nrdR"
                     /locus_tag="PLK08_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPS53367.1"
                     /translation="MRYGEVRGVIMQCPFCGQDNDKVIDSRSSDGGRVVRRRRECLVC
                     EKRFTTYERVEETLRLSVIKKDGSREPYNREKMYDGLEKSCYKRPISTDQLRQIVEAA
                     EEKIFRTFDKEVPSSFIGDTLASRLREVDKIAYVRFASVYRDFTDVGELINEAQEVKQ
                     DPVITPGQKGLFNE"
     gene            complement(1006..2502)
                     /locus_tag="PLK08_07440"
     CDS             complement(1006..2502)
                     /locus_tag="PLK08_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53368.1"
                     /translation="MKPYECYLKSHGQLQIELHARYPLKPGEKKVDYFLDMYIFTPSQ
                     LDMNKKHYGVDQFLHDLQCYTRYTHPSLPLARLLDPDCPISPLTKIRKALDIESAYND
                     DMNADELLYELRTLVNIYRRELREMRNAIRQRIDGGASSEELRQKMEAMLGEEDELLL
                     QVRNLHGRFLDPRIPEMLREGLRNADEALSIKTEKELFRLYHMIENRNDFRDLSDSIL
                     ARLKAERLDRMQAGYATIISTGTPAENDRFNYYDSMLKRWAQSTMYMDVTPSKIASHV
                     GNIVAGFAAGLAMTFAVLVTIFTQSNFKQNSLPWALLIIFAYIVKDRLKELTRNIFVA
                     SLPGLISDVSTKLVDPANRRQVGVTRSWVRFCGPDDIDENILRFRDAHSLPFRKLLPP
                     EDIIHYQKRIRLDSDSFLAAHSRIDSITEILRLNMRKWLSEMDDPINVLEYLDDNGQP
                     AIIESNRGYHVNIILHLSSDQKGMDDILKRYRLILNRNGIIGVEELEL"
     gene            complement(2545..3792)
                     /locus_tag="PLK08_07445"
     CDS             complement(2545..3792)
                     /locus_tag="PLK08_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HPS53369.1"
                     /translation="MKLPELLLPAGSLSKLAIAYEYGADAAYVGAAGFSMRPDGASFT
                     PDQLLAAVDIAKTAGRKLYVAVNSLIFESELEELTQWLTETRETAFDAIIVADCGAAA
                     MVRRHRPDVELHISTQMSTANSQAVNFWAAAGANRVILARECTLQHAAHIVRHARTDV
                     EIFVHGAMCVAVAGRCLLSAHLAGRSGSRGDCKHACRWDWQLVEAKRPGETLPIFEAG
                     GQSFLLGSNDLCLIEHIPQIVATGVKSLKIEGRMKGEYYVAVVADVYRQALDEYAKLG
                     EAWQCRPQWLTELESVTHQPYSTGFAFGYPTERPHSLQSGGRVPGTHDIAAIIEKNCD
                     GGVFLADAKRPFSAGDELEWLGRGCRRKSVTVKTVFTLDGQPVTRTHVGVKYLVTFDS
                     QFDDVPKNSILRKLAVVSSPENC"
BASE COUNT          814 a         1015 c         1043 g         1007 t
ORIGIN      
        1 gtgaatgctt gaggtaaaaa tgaatagcgg cggcaagttc gccggcgaaa gtgaaatctt
       61 cacagccggc agcgaatatg ccgcgggcaa tcacgccgcg caatcgctgt tcgtcgaatg
      121 cctccacgct cccgtcacgc ttgcgaacct tgatagcaac tgttttgtcc gacattgccg
      181 gaatctccat attccacgtc ggccatcctg gccttgcaat ttttcaataa ttattttcgt
      241 aattccacga ttttcagccc gcagagaatt ttcattctct tttcagaatc attcgttgaa
      301 gaggcctttc tggcccggtg ttatgaccgg atcctgcttg acctcctggg cttcgtttat
      361 aagctcgccg acatccgtga aatcgcgata tacgctggcg aaccgcacgt aggcgatttt
      421 gtcaacttca cgcagcctgc tggcgagggt atcgcctatg aaactgctgg gcacttcctt
      481 gtcgaatgtg cggaagattt tttcttcggc ggcttcgact atctggcgaa gctggtcggt
      541 gctgatgggc cgtttgtagc aggatttttc caagccgtcg tacatctttt cccgattgta
      601 cggctcacgc gaaccgtcct tctttatgac cgacagccgc agcgtctctt ccacacgttc
      661 atacgtggtg aaacgttttt cgcagacaag acactcccgc cgccgcctga cgacacggcc
      721 gccgtcactg ctgcgggaat ctatgacttt gtcattatcc tgtccgcaga atggacactg
      781 catgattaca cctcgcacct cgccatatcg catgacgatg ccaacttcag cggctgtctg
      841 tacttccggc gacgcaaaaa tcggcttacc atacgcttcc tacatatagt agttttctga
      901 gtttaatcgt caagatgtgg tatgtcaagc agaattggct aaaatatcgc ttaaaaaccc
      961 ctatttatag cgaaaaatct cgccgcaaag gtggcggcat catgtctaca gctccagctc
     1021 ttccactccg atgattccat tacgattgag aatcagccgg taacgtttca ggatatcgtc
     1081 catgcctttc tggtcgctgc tgaggtgcag aattatgttt acgtgatagc ctctgttgga
     1141 ttcgattatg gccggctggc cgttatcgtc cagatactcc agcacgttta tcgggtcgtc
     1201 catttcgctc agccacttgc gcatgttaag tcgcaatatt tcagttatgg aatcaatgcg
     1261 gctgtgcgcg gcgagaaagc tgtcgctgtc gagcctgatg cgcttctggt aatgtatgat
     1321 gtcttcgggc ggaagaagtt tgcggaacgg cagtgaatgt gcgtcgcgga atctcaggat
     1381 gttttcgtcg atgtcatccg ggccgcagaa ccgtacccat gaacgcgtga cgccgacctg
     1441 ccgccggttg gcagggtcga cgagcttggt ggatacgtcg gaaatcagcc ctggcaggct
     1501 ggcgacgaaa atattgcggg tcagctcttt cagcctgtct tttacgatgt atgcgaaaat
     1561 aatgagcagt gcccacggca gtgaattctg tttaaaattg ctttgggtga aaatggttac
     1621 aagaacggca aaggtcatgg caaggcctgc cgcgaaaccg gcgacgatat tgcccacatg
     1681 cgaggcaatt ttcgacggcg tgacatccat gtacatagta gactgcgccc agcgtttcaa
     1741 catgctgtcg tagtagttga acctgtcgtt ttcggcgggt gtgccggtgg aaattatagt
     1801 tgcgtaaccg gcttgcatgc ggtcaagccg ctctgccttc aatcttgcca aaatgctgtc
     1861 ggataaatcc ctgaagtcgt tacgattctc aatcatatgg tataatctga aaagctcttt
     1921 ttccgttttg atgctcaatg cttcgtccgc attgcgaagg ccttcgcgga gcatctccgg
     1981 tatgcgaggg tcgagaaatc tgccgtgcag attgcgtacc tgcaacagca attcatcttc
     2041 ttcgccgagc attgcctcca ttttctgccg cagttcttcc gaggaagcgc ctccatcgat
     2101 tctttgtctg attgcatttc gcatttcccg caattcgcgg cggtatatgt tcacgagcgt
     2161 gcggagttcg tagagaagtt cgtcggcatt catgtcgtcg ttgtatgcag actcgatgtc
     2221 gagggctttg cgtattttcg tcagcggact tatcgggcag tccgggtcga ggagtcgtgc
     2281 aagcggcagt gacggatgcg tgtatcgcgt gtaacattgc aggtcgtgca ggaactggtc
     2341 gacgccgtaa tgttttttgt tcatatccag ttgcgacggt gtgaaaatgt acatatccag
     2401 gaagtaatcg accttttttt cgcctggttt gagcggatat cgcgcatgca gttcaatctg
     2461 cagttggccg tgcgatttca gatagcattc atagggtttc atatactcat ccgtcccgac
     2521 agattccgcc gtcgaactct aacactaaca attttccgga gacgatacaa cggccaattt
     2581 tctcagaatt gaatttttgg ggacatcatc gaattgcgag tcgaacgtaa cgaggtattt
     2641 tacgccgacg tgagttctgg tcaccggctg gccgtcgagt gtgaagacgg ttttgaccgt
     2701 gacgcttttt cgccggcagc ctctgcctag ccattccagt tcgtcgccgg cagagaaggg
     2761 tctttttgcg tcagccagga atacgccgcc gtcgcaattt ttttcgataa tcgcagcgat
     2821 gtcatgcgtg ccaggcactc tgccgccgga ttgcagagaa tgcggccgtt ccgtcgggta
     2881 tccgaacgca aagccggtcg agtaaggctg atgcgtcacg ctttcgagtt cagttagcca
     2941 ttggggccga cactgccatg cttcgcccag ctttgcgtat tcgtccagcg cctgacggta
     3001 tacgtcggct accacggcga cgtagtattc gcctttcatc ctgccctcga ttttgagcga
     3061 ttttacgccc gtggcgacga tttgcgggat gtgttcgata aggcacaggt cgttcgagcc
     3121 gagcaggaaa gattggccgc ctgcttcgaa aatcggcagg gtttcgcctg gccgttttgc
     3181 ctcgacaagc tgccagtccc accggcaggc gtgtttgcaa tctccgcgag agccgctccg
     3241 gccggccaga tgcgccgaga gcagacatcg gccggcgact gcgacgcaca ttgcaccgtg
     3301 gacgaatatc tcaacgtcgg ttctggcgtg tcgcacgata tgggcggcgt gttggagcgt
     3361 gcattcacga gccagtatca cgcggttggc gccggcggcg gcccagaagt tgacagcctg
     3421 cgagttggct gtcgacatct gcgtggatat gtgcaattca acgtccggcc gatggcgtct
     3481 gaccatcgcc gccgcaccgc agtcggctac tattattgcg tcaaacgctg tttcccgcgt
     3541 ctcggtcagc cattgcgtaa gttcttcaag ctcgctctca aaaatcagcg aattgaccgc
     3601 gacgtaaagt tttctgccgg cggttttggc gatatccaca gcggcgagca actggtcggg
     3661 agtgaaactg gcgccgtcgg gacgcatgga aaatcccgca gcgccgacat aggctgcatc
     3721 ggcgccatat tcgtatgcga tggcgagttt ggacagcgaa ccggcgggta ggagtaattc
     3781 tggcagtttc atggtaaaaa cctgcgtaat tcaaatttta taaacacgga ttcagaggcc
     3841 gttctcaatt accctgcgga cgcaaaaaat aagatattt
//
LOCUS       DAONFI010000221         3871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28499_length_3871_cov_4.300052, whole genome shotgun sequence.
ACCESSION   DAONFI010000221 DAONFI010000000
VERSION     DAONFI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28499_length_3871_cov_4.300052"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLK08_07450"
     CDS             complement(<1..231)
                     /locus_tag="PLK08_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPS53370.1"
                     /translation="MKTDDNCIFCKIVAGKIPSTKVYEDDDSLAFMDIGPVSPGHVLL
                     ICKEHYEKIHEVPAEVAAKTLAVLPKLVNAVQT"
     gene            complement(504..1061)
                     /gene="rplQ"
                     /locus_tag="PLK08_07455"
     CDS             complement(504..1061)
                     /gene="rplQ"
                     /locus_tag="PLK08_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPS53371.1"
                     /translation="MRHRVAGKQLSRTTSHRKAMRRNMAASLFEHGAIRTTEVKAKEL
                     RSFAEKLITIAKQGTLHARRQVIQMLRDRDIYAVDPKTQDSDPTEQTVVQKLFSEIAP
                     RYAQRQGGYTRLVRIADRRIGDGGVQMIVQLVEEGAKREKNTANTKRRARVSKLYAAA
                     DVAVAGKTSAAPAKAAEEAPEKTAE"
     gene            complement(1337..2353)
                     /locus_tag="PLK08_07460"
     CDS             complement(1337..2353)
                     /locus_tag="PLK08_07460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPS53372.1"
                     /translation="MRIRWRGLELPTRVVLDQEVSTPTYGKFAIEPFERGFGITVGNS
                     LRRVLLSSLEGAAVTRVKIKGATHEFTTLPGVLEDIADIILNIKTLVIKSYTSEPKTM
                     TLKSSKKGAVTAGMIECDSSIEIINKDLVIATLTDDVEFDIEFTIETSRGYVPAEELA
                     AGEQELGVIEVDAFFSPVTRVRYRTEDTRVGQKTNYDKLIMEIWTNGTITPEMALVES
                     GKILRKHLNPFVQYFEMGQQIPEGGMIETGNEVDEELNRKLGMSVQELELSVRASNCL
                     ESAQIETVGQLVKMAESELLKIRSFGKTSLREIKRKLADLGLSLGMNVELPQPEATVA
                     AEGE"
     gene            complement(2497..3120)
                     /gene="rpsD"
                     /locus_tag="PLK08_07465"
     CDS             complement(2497..3120)
                     /gene="rpsD"
                     /locus_tag="PLK08_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPS53373.1"
                     /translation="MGRYTGPTDRLSRREGINLMLKGARSLNGKSERRMQTPPGMHNW
                     RKGRLSDYGVRLREKQKVKRYYGVRDKQFMNYFVKAENTKGNTGTIMLSLLERRLDNV
                     IYKLGMAPSRPAARQTIVHGHIYVNGRKVNVPSYQVNVGDKISVKPSEKTKALIRSRL
                     EELGEPRLQNWLSLDMSQLAGEVVALPTRDDVMIPVEDQFLVEFCSR"
     gene            complement(3287..3688)
                     /gene="rpsK"
                     /locus_tag="PLK08_07470"
     CDS             complement(3287..3688)
                     /gene="rpsK"
                     /locus_tag="PLK08_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPS53374.1"
                     /translation="MAQSQKATATTTKKVKKHVARGIVHVRATFNNTQVTITDLNGDV
                     ICIESAGSVGGFKGSRKSTPFAASRASESAANKAKKIGMTEVEVKVKGPGSGRESAVQ
                     ALQAAGLKVLSIEDTTPIPHNGCRPRKRRRV"
     gene            complement(3692..>3871)
                     /gene="rpsM"
                     /locus_tag="PLK08_07475"
     CDS             complement(3692..>3871)
                     /gene="rpsM"
                     /locus_tag="PLK08_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014245794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPS53375.1"
                     /translation="NLRREISQNIQRLKAIGCYRGLRHRRGLPVRGQRTKTNARTRKG
                     PRKTVAGKKSVKEMR"
BASE COUNT          811 a         1020 c          962 g         1078 t
ORIGIN      
        1 agtctgaacc gcattaacca gtttcggcag aacggctaac gtttttgccg ctacttcggc
       61 gggcacttca tgaatcttct cgtaatgttc cttgcatata agaagaacat ggcccggcga
      121 aacagggcct atatccatga acgccagtga atcatcatcc tcatatactt ttgtcgacgg
      181 tattttgccg gcaactatct tgcagaaaat gcagttatcg tctgttttca tggcggttct
      241 ccttgcatat cgcgaatata gagcagtttt caaaattatt tcctttcgaa aaccgctcca
      301 gaatgattct cgaaagcaat tttttgaaaa ccactctaat agcgaaggcc gctgtcctgt
      361 ttttttcggg acaacggcct gttcggtttt tgcagaatcc accgcgtctc acttacacgg
      421 ctgggccatc agctctatta ttcacccttt atataacagc aaagattaaa ttatacatcc
      481 caaatccctg aaatgggaga aaattactct gcggtttttt ccggtgcttc ctcagccgcc
      541 tttgccggcg ctgcagaggt ttttccggca acggcaacat cagctgcggc ataaagtttg
      601 ctcacacggg cacgacgctt cgtgttggcg gtattttttt cgcgttttgc gccttcttct
      661 acaagctgca ctatcatctg tacgccgcca tcgcctatcc ggcggtcggc aatgcgaaca
      721 aggcgggtat aaccgccctg ccgctgggca taacgcgggg ctatttcgct aaaaagtttc
      781 tgaaccacgg tctgttccgt aggatcgctg tcctgcgttt tggggtcgac ggcatagata
      841 tcgcgatcac gaagcatctg tatgacctgc cggcgggcat gaagcgtgcc ctgcttggca
      901 atggttatga gtttttcggc gaaactgcgc aattccttgg cttttacctc ggtggtgcgg
      961 atagcaccgt gttcgaatag cgatgccgcc atgtttcgcc tcatcgcctt gcggtgcgac
     1021 gtggtacggc ttaattgttt tcctgctaca cggtgacgca ttgttacgtc ctctttccta
     1081 atctgggcaa cggcttttcc cgtcgcggtt ttttatttct ttattttttc tctagtaaaa
     1141 accgtagttt catatttatc aggttgtggc ttgtctgcct tagggcggat actttaaaaa
     1201 catctacggt ttataacagt ttttgaaaag aatattttta ggtaaatctc ttttaaaaac
     1261 cgccgaatta ctaaagtcat attaaaaact tatttcagcc gccgattaaa acatcaacgg
     1321 ctatcggcaa ttaaaatcat tcgccttcag cagcgacagt ggcttccggc tggggcagtt
     1381 ccacgttcat gccgagcgaa agtcccaaat cagccagctt gcgtttgatt tcacgcaatg
     1441 acgtcttgcc gaagctgcgg attttcaaca attccgattc agccatttta acgagttggc
     1501 cgacggtttc gatttgagcc gattcaagac agttgctcgc acgaactgag agttccaact
     1561 cctgcacgct catgcccagc ttgcggttaa gttcctcatc gacttcattg ccggtctcaa
     1621 tcatgccgcc ttcaggaatc tgctggccca tttcgaaata ctgcacgaac gggttaagat
     1681 gtttacggag aatcttgccc gattcgacaa gagccatttc cggcgtaata gtgccgttcg
     1741 tccagatttc cattatgagt ttgtcatagt tggttttctg gccgacacgg gtatcttccg
     1801 tgcgataacg gacgcgggtc accggcgaga aaaaggcatc gacttctata acgccgagtt
     1861 cctgctcgcc ggcagcaagt tcttcagccg gaacgtaacc gcggctggtt tcgatggtga
     1921 attcgatgtc aaattcgacg tcgtccgtca gcgtcgcaat caccaggtct ttattgatga
     1981 tttcgataga tgaatcacac tcaatcatgc cggctgttac agctcctttt ttggagcttt
     2041 tgagcgtcat ggtctttggt tcgctggtat acgactttat gacaagtgtc ttgatattca
     2101 gaatgatgtc ggcaatatct tccagcacgc ccggcagtgt ggtgaattca tgcgtagcgc
     2161 ccttgatttt cactctcgtg acggctgcgc cttccaggct gctcagaagc acacgacgaa
     2221 gggaattgcc cacggtaatg ccgaagccgc gttcaaacgg ctcgattgca aactttccat
     2281 aagtaggcgt cgacacttcc tgatcgagga ctacacgggt cggcagttct aatccacgcc
     2341 aacgaattct cattttcaac ctccgtccct gtcgggactt tagcgacctt ttgccttaac
     2401 gtccggcagg tctgggttag caccggtcgg tttatcaggc acggcaccgt gccgcggcct
     2461 atctgctcgt ccggtgattg cctttgctgt gttttattaa cggctgcaga actcgacgag
     2521 gaactggtct tcgacgggta tcatcacatc gtccctggtg ggcagggcca cgacctcgcc
     2581 ggcaagctgg ctcatatcga ggctcagcca gttttgcaga cgcggttcgc cgagctcttc
     2641 aaggcggctg cgaatgagag ccttggtttt ttcagacggc ttgaccgaaa tcttgtcgcc
     2701 cacatttacc tgatagctag gcacgttgac ttttcggcca ttgacgtaaa tatggccgtg
     2761 cactatggtc tgccgtgcag caggacgact aggcgccatg ccgagtttgt aaattacatt
     2821 gtccagacga cgctcaagaa ggctgagcat gattgtgccg gtgttaccct tggtgttttc
     2881 agccttgaca aagtagttca tgaactgctt gtcgcgtacg ccgtaatagc gtttcacttt
     2941 ctgcttttcg cggagtcgta ctccgtagtc gctcagtcgg cctttgcgcc agttatgcat
     3001 gccaggcggg gtctgcatac ggcgttcgct tttgccgttc aggctgcggg cgcctttgag
     3061 catcaggtta atgccctcac ggcgtgaaag cctgtcggta ggtcctgtgt aacgtcccat
     3121 caaaaatctc ctgaattgca cctgttcgcc agatgcgacg ttcagcaact acaagatatg
     3181 cccgcacggg taaaatccgg ttttccggca cccgtcgggc gtggcaatca gcgggattct
     3241 gctgaataaa tcccgcagcc gttactataa ataagcaaat cgcgtatcag acgcgacgtc
     3301 ttttgcgagg gcggcatccg ttgtgcggta tcggggttgt atcctcgatg cttagcactt
     3361 tcaggccggc tgcctgtagt gcctgcacgg cactttcacg gccgctgcca ggccccttta
     3421 ccttgacttc aacttcagtc atgccgattt ttttggcttt attggctgcc gattcgctgg
     3481 cgcggctagc agcaaaaggc gtactcttgc gagaaccctt gaacccgcca acagaacctg
     3541 ccgattctat gcatatcacg tcgccgttga gatcggttat cgtcacctgc gtgttgttga
     3601 acgtagcccg cacgtgaact atgccgcgcg cgacatgctt cttgaccttc ttggtagtag
     3661 tagccgtagc tttctgtgac tgagccatgg tttatcgcat ctccttgaca gacttcttgc
     3721 ctgcaacagt tttgcgcggg cccttacgag tacgcgcgtt tgttttcgtg cgttggccgc
     3781 gaaccggaag gccgcgacga tgacgcaaac cgcgataaca accgatggct ttgagacgct
     3841 ggatgttctg cgaaatttcg cgacggaggt t
//
LOCUS       DAONFI010000222         3851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28712_length_3851_cov_3.920443, whole genome shotgun sequence.
ACCESSION   DAONFI010000222 DAONFI010000000
VERSION     DAONFI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28712_length_3851_cov_3.920443"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLK08_07480"
     CDS             complement(<1..793)
                     /locus_tag="PLK08_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPS53376.1"
                     /translation="MSRLLIKGGRVIDPANGINRICDVLIADSRIKKVGTVNKSDAAG
                     AEAINAKGMIVAPGLIDIHVHCREPGHEEEETIASASAAAVAGGFTTICAMPNTHPAQ
                     DDERAINYVLERSAEASLANVLPVGCITKNRDGKELAEMALMCEAGVVAFSDDGNGVD
                     DSKIMHRALQYASMLGISLMQHCQDSDLFSGVMNSGPVAVRLGLGGIAAACEEIMLRR
                     DLELVAHTKAPYHVQHASTAGSVELIRQAKSQGLPVTAEATPHHLL"
     gene            complement(843..1952)
                     /locus_tag="PLK08_07485"
     CDS             complement(843..1952)
                     /locus_tag="PLK08_07485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPS53377.1"
                     /translation="MKVPLLDLTAQYAGLKSQIMPAVEAVLDSQMVCNGPAVRQFESQ
                     IASYCGSKHGVGVSSGTDALLCALMALGIGPGDEVIIPPFTFFATAGVVWRVGAKPVF
                     VDIQPDTFNIDPDLIAPALTPRTKAIIPVHLFGQTADMDEIMGIAASHDLYVVEDAAQ
                     SIGSAYKGHRACSIGTVGCLSFYPTKNLGACGDAGMCITGDDEIAAKLERTRNHGQGG
                     TYMHETVGGNFRMDTVQAAILSVKLAVLDDWHEGRRRNAAIYDEKLAGLDGIILPHVR
                     RDNYMIYNQYTIRVKNSRRDALKKFLADNEIASGVYYPLSLHQQECFASLGYRQGDFP
                     VSEMAAAEVLSLPITGEISPQQIEFVAQKVREFAS"
     gene            complement(2014..2805)
                     /locus_tag="PLK08_07490"
     CDS             complement(2014..2805)
                     /locus_tag="PLK08_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HPS53378.1"
                     /translation="MKLFTKEQLIAEFKKIAQRGWIENLRHGNAGGVGNTLEELLGIE
                     ENNLPIPNASEWELKAQRMGTTSLTTLFHTEPSPRAVKFVPQFLLLKYGWKHKEAGKK
                     YSHDEMSFRQTIHGKSYSDRGFIIKIDRIAKKLLVSFDCRKVSERHIDWLAQVKIKTG
                     LEELSPQPYWGFDDLASKARTKLLNCFYVQAEVKKENGKEFYKYSRIVMLKKFSFTAF
                     LDEIEKGNVLVDFDARTGHNHGTKFRMRQNCLPQLYESTTEISLT"
     gene            complement(2802..3602)
                     /locus_tag="PLK08_07495"
     CDS             complement(2802..3602)
                     /locus_tag="PLK08_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPS53379.1"
                     /translation="MLGGVVNNINNYLNKIICGDCVEIMKKLPDNSIDLVVTSPPYNL
                     KNSTGNGMKSCTTSGKWAGAALQNGYTHHDDCMPHKQYVSWQRNCLSEMFRLIKDDGA
                     IFYNHKWRVQKGLLQDRQDIVGGFPVRQIIIWRRKGGINFNPGYFLPTYEVIYLIAKP
                     KFKLIPKANAYGDVWEFTQEMNNQHPAPFPVALIDRIVSSTDANIILDPFMGSGTTAV
                     VAMGLNRKYIGIELSPEYCKMAETRIKLSKTDCEVRKNRQLTLFQHES"
BASE COUNT          982 a          943 c          831 g         1095 t
ORIGIN      
        1 gaagcaggtg gtgcggcgta gcttcggcgg taacaggcag gccttgcgat ttcgcctgtc
       61 ggatgagttc cacactgccg gctgtcgatg cgtgttgtac gtgatacggc gccttcgtgt
      121 gagccacaag ctccaggtcc cgccggagca tgatttcctc gcaggcggcc gcgatgccgc
      181 caagtcccag ccgcactgct acgggccctg aattcatgac cccgctgaag aggtcggaat
      241 cctggcaatg ctgcatgagc gaaatgccga gcatggacgc atactgcaaa gcgcgatgca
      301 ttattttgga atcgtccacg ccgttgccgt cgtcgctgaa cgcaactact cccgcttcgc
      361 acatgagagc catttccgcc aattccttgc cgtcgcgatt tttcgtaatg cagccgaccg
      421 gcagaacgtt agcaaggcta gcctccgcgc tgcgttcgag aacgtaattt atcgcccgtt
      481 cgtcatcctg ggccggatgc gtattcggca tggcgcatat ggtggtgaaa ccgccggcga
      541 cggctgccgc cgatgccgac gcgatggttt cctcttcctc atggccgggc tcgcggcagt
      601 ggacgtgaat atcaatcagg ccgggggcta ctatcatgcc tttggcgttt atggcctccg
      661 cgccggcggc gtccgattta ttgactgtgc cgactttttt tatacggctg tcagctatga
      721 ggacgtcgca aattctgttt attccgttcg cagggtcaat gacccgcccg ccttttataa
      781 gcaatctgct catggttgtc tccatagtcc ggctgcgtgc atgataacac cgtcggccgc
      841 cgttacgaag cgaattctct gaccttttgg gccacaaatt ctatctgctg cggagaaatt
      901 tcgccggtta tcggcagtga gagcacctcc gcagccgcca tctcgctgac agggaaatca
      961 ccctgccggt atcccagcga cgcaaaacat tcctgctgat gcaggcttag cggataatag
     1021 acgccgctgg caatttcatt gtcagccagg aattttttga gtgcgtcgcg acggctattt
     1081 ttgacgcgaa tggtgtactg gttataaatc atgtagttgt cgcgacgaac gtgtggcagg
     1141 atgatgccgt caaggccggc gagtttttca tcgtaaatcg ccgcgttgcg ccggcggcct
     1201 tcgtgccagt cgtcgagcac ggctaatttt accgaaagga tagcagcctg gacggtatcc
     1261 atgcggaaat taccgccgac tgtctcatgc atgtaagtgc cgccctggcc gtggttacgg
     1321 gtacgctcca gcttggcggc aatttcgtcg tcaccggtta tgcacatgcc ggcgtcgccg
     1381 catgcgccga gattcttcgt gggataaaaa ctgaggcagc ccacagttcc tatcgaacac
     1441 gcccgatggc ccttgtaggc agaacctatc gactgggcgg cgtcttccac cacatacaaa
     1501 tcgtgcgacg ccgcaatgcc cataatctcg tccatgtcag ccgtctggcc aaaaaggtga
     1561 accggaatta tcgccttcgt tcgaggcgtg agggctgggg cgatgaggtc ggggtcgata
     1621 ttgaaggtat cgggctgaat gtccacgaaa accggctttg ctccgactcg ccagacaacg
     1681 ccggctgtcg caaaaaacgt aaacggcgga ataatgactt catcgcccgg ccctatgcca
     1741 agcgccataa gcgcacaaag cagcgcatcc gtgccgctgg aaacgcctac gccgtgcttt
     1801 gagccgcagt atgatgcaat ctgcgactca aactgacgga cggctgggcc gttgcagacc
     1861 atctgggaat ccaggacagc ttcaacggct ggcataatct gcgacttcaa tccggcgtat
     1921 tgtgcagtaa ggtcaagcaa tggaaccttc attcccaaat cctttcaatc cgcttaaaat
     1981 gcaggatttc taattaataa ataaaagata tttctaagtc aacgatatct cagttgtact
     2041 ttcatacaat tgcggcaaac agttttgtct catccgaaat tttgtaccat gattatggcc
     2101 agtcctagca tcaaaatcga ctagaacatt acctttttct atttcatcca aaaaagcagt
     2161 aaaactaaat ttttttaaca tcactattct ggaatactta taaaactctt ttccattttc
     2221 tttttttact tcggcttgca cataaaaaca gttaagaagt ttcgtacgag ctttactagc
     2281 taagtcatca aatccccaat aaggttgggg tgaaagttct tctaatcctg ttttaatctt
     2341 gacttgcgca agccagtcta tatgtcgttc cgaaactttt ctgcaatcaa aagataccaa
     2401 taactttttt gcaattctgt caatttttat gataaaacct ctatcactat acgacttacc
     2461 atgaatcgtt tgccggaagc tcatttcatc atgcgaatat ttcttcccag cctctttatg
     2521 tttccagcca tatttcaata ataaaaattg tggcacaaat tttactgcac gaggagaagg
     2581 ctcggtatga aataaagtag ttaacgatgt tgtccccatt cgttgtgctt taagttccca
     2641 ttccgaagca tttggtatag gcaaattatt ttcttctata cctaacaatt cttccagagt
     2701 gttgccaaca ccgcccgcat ttccatgcct taagttttct atccaacctc tttgagcaat
     2761 ttttttaaac tcagcgatta gttgttcttt tgtaaataat ttcatgattc gtgttggaaa
     2821 agagttaatt gtctattttt ccgtacttca caatcagttt tacttaattt aattcttgtt
     2881 tcagccatct tacaatattc aggcgataac tctattccaa tgtactttcg atttagtccc
     2941 atagcaacaa cagctgtagt gccacttccc atgaacggat ctaaaatgat attcgcatca
     3001 gtggaactaa ctattcggtc tattagggca acaggaaatg gtgctggatg ttgattattc
     3061 atttcctgtg taaactccca aacatcacca taagcattag ctttgggaat caatttaaat
     3121 ttgggtttag caattaaata tatgacctcg taagtaggca gaaaataacc tggattgaaa
     3181 tttatcccac cttttcgtcg ccagataata atttgtcgga caggaaaacc accgacaata
     3241 tcttgtctat cctgcaacag ccctttttgt actctccatt tatgattata aaatattgct
     3301 ccgtcatctt ttatcaaacg gaacatttct gaaaggcaat ttctttgcca acttacatac
     3361 tgcttatgcg gcatacagtc atcatggtgc gtataaccgt tttgtaaagc tgcgccagcc
     3421 catttaccac tggtagtaca agacttcatc ccatttccag tagaattttt taaattatat
     3481 ggaggtgatg taactactaa atcaatagaa ttatctggta gttttttcat tatttctaca
     3541 caatcgccac agataatttt atttaagtaa ttattaatat tattcacaac tccacctaac
     3601 atatatttga aaaccttgtt aagctgaaga caaaaatttt ttattaagtt attcaaactg
     3661 accaacttac gacttttcgc atatgaggct aaaaggtaac gacatatttt gcaataaaat
     3721 acattactgc gactcacaaa ggcgacgtcg cagaaccatt tgtcatggat atcgtaactg
     3781 cttgcaaaat aagtcgttac cgagccgtat agcaagaatc ggtcactttt aataagttat
     3841 tataatgttt g
//
LOCUS       DAONFI010000223         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28739_length_3849_cov_4.016869, whole genome shotgun sequence.
ACCESSION   DAONFI010000223 DAONFI010000000
VERSION     DAONFI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28739_length_3849_cov_4.016869"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="PLK08_07500"
     CDS             complement(<1..1907)
                     /locus_tag="PLK08_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPS53380.1"
                     /translation="MKEQARGRKVAILGAVTQAAVLVAAIIVWLLTGSQAMLNSVWFL
                     LGGLSLWVMTAVLFYARQKAWQEEMELREIAASGQADTIFDEEHTAEFQPAARRLKFM
                     QKWLVPILTLLLAGYYIATGVWMLQIMGRTAEQTELTSIAPGIIFLLISGFLLFLMSK
                     YAVGMSSNRQWRLLHAPGSYSTVCALMLALLAVSLFSTTTKFAAVHQIMAWIIVAAGM
                     VYSVESVLNIVLDIYRPRRPGDEYRPGYDSRIFALVAEPGKVGHSIADTLNYQFGFEV
                     SKTWFYKLVSRAFVPLIIFGFVVMILLSGVVIVSPGEVFVIKTLGRVPSKENTLGPGM
                     HFKWPWPIAIAQRFNTGLIQQLILGVGGMVETQKTSDGQELVLWTQVHGYAQQEEQDF
                     LIAVPPRESSKEDAPSTISIIKLVVALQYQITDVYKYGYNYVNAADTLKCIAEQEMVE
                     YCASATLDTKMPGDDTSRPQAIMNSGRLEAAANLQKLIQKRLDDADMGVKILFVGIIS
                     AHPPAEAVPQFEKVLEAERLQDQQRYEAQAKASKILAGISGDPDKAMKLFVDLYRAEI
                     FDKLNMEKGTPTFTVSINEFIRKATEQVNAMTKEIDREKLLGRDVPGMENLRNAYENL
                     RSQLVNAQTNP"
     gene            complement(2035..3477)
                     /locus_tag="PLK08_07505"
     CDS             complement(2035..3477)
                     /locus_tag="PLK08_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53381.1"
                     /translation="MIKFYAIAKNTFLQTIRQPIFGVMIFLTFAILVMSLPLSGMTMG
                     TDYEKSDERMMENVGLSTLLIMGFITAALSASSAIGREIEDRTALTVASKPVARATFV
                     LGKFSGITLAVMIAYYLMSIVFLLTVRHKVVSAASVPINWPVIIFGVSAFAISLILGG
                     LGNYMFNWSFIATGCMALLVFMTVAFGLSCIIDLHWQFVNIADTFSDDNIKPSLILAL
                     ILIFLAIVLLTAVATAASTRLGQVPTLLITFLILAAGSWYLLINARLNEYFVGPGREA
                     PIINVLCWLLPNMTVFFPLDALTSNKPIPVSFVGMAACYCACYTAAVLFVGIALYQTR
                     QMEAQAASSTMPSALSLLSGFGRIAGIIFVIMAVIMATLPANHTTGGFVTIGLLFVGG
                     GTLWYVTRVLGRGRKWSWRLLTLLSGILLVRGIVLWQAGSYAQWLFMGQSTPGILTLS
                     LVSALVFLLLMLPKTRRHVKALNMISTDEF"
     gene            complement(3477..>3849)
                     /locus_tag="PLK08_07510"
     CDS             complement(3477..>3849)
                     /locus_tag="PLK08_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HPS53382.1"
                     /translation="DAPALAAGDLSIAMGAAGSDVAINSANIALMNTDLRRLPFLIEL
                     SRATSRVVHQNLLFGVCFIILFMIGAGLGKIPPIWGAILHTVSATVVIFNSARLVRMG
                     EYLHLDEKTGEDQLAKVERIR"
BASE COUNT          999 a         1061 c          882 g          907 t
ORIGIN      
        1 atgggattcg tttgcgcgtt gacaagttga gagcgcagat tttcataagc gttccggagg
       61 ttttccatgc cgggtacatc acggccgagg agtttttcgc ggtctatttc cttcgtcatg
      121 gcgttgacct gctcggtggc tttgcggatg aactcattaa tagatactgt aaaagtcggc
      181 gtgccttttt ccatgttaag cttgtcaaaa atttcagcgc ggtaaagatc cacaaaaagt
      241 ttcatcgcct tgtcagggtc gcctgaaatg ccggcgagta tcttgctggc tttggcttgc
      301 gcctcataac gctgctggtc ctgcagacgt tctgcctcca ggactttttc aaattgcgga
      361 acggcttcag ccggcggatg cgcactgatt atgccgacaa acaggatttt tacgcccata
      421 tccgcgtcgt ccagtcgttt ctgaatgagt ttttgcagat ttgcggcggc ttcaagacgg
      481 ccggagttca ttatagcctg cggccgcgaa gtatcgtcgc caggcatttt cgtatctagc
      541 gtcgctgacg cacaatattc caccatctcc tgctcggcta tgcatttgag cgtatcagcg
      601 gcattgacgt aattgtaacc gtatttgtaa acgtccgtta tctggtactg caacgcgact
      661 accagtttta ttatgctgat tgtggatgga gcgtcttctt ttgaagattc gcggggcggc
      721 acggctatca ggaagtcctg ctcctcctgc tgggcataac cgtgaacctg cgtccagagc
      781 actaattcct ggccgtccga agttttctgc gtttcaacca taccgccaac accgagaatc
      841 agttgctgta taagtccggt attaaaccgt tgtgcaatgg caatcggcca aggccatttg
      901 aaatgcattc caggcccaag cgtgttttct ttcgacggaa cacggcccag cgtttttatg
      961 acaaatactt caccaggcga gactatcaca acgccgctga gcaatatcat cacgacaaaa
     1021 ccgaaaatta tcagcggcac gaatgcgcgg cttacaagtt tgtaaaacca cgtctttgac
     1081 acttcgaagc cgaactgata attaagagta tcggcgatgg aatggccgac tttgccaggc
     1141 tcagccacaa gggcgaaaat gcggctatca tagccgggcc gatattcgtc gcccggccgc
     1201 ctcggccggt aaatatccaa aacaatattt aaaacgctct caaccgaata taccataccg
     1261 gcggctacaa taatccatgc cattatctgg tgcacagccg caaatttcgt agtcgtactg
     1321 aaaagactca ccgccagcaa cgccagcatc aatgcgcaaa cggtagaata actcccaggc
     1381 gcatgcagca gtcgccactg tctgttggaa ctcatgccga cggcgtattt gctcattaga
     1441 aacaacagaa acccgctgat gagcagaaaa attatgcctg gagctatcga cgtcaattcc
     1501 gtctgttcag ccgttctgcc catgatttgc aacatccaga cgccggtggc gatgtaatat
     1561 cccgccagca atagcgtcaa tatcggcaca agccacttct gcatgaattt cagacgtctt
     1621 gcggcaggtt gaaattcggc ggtatgttct tcatcaaaaa tcgtatccgc ctggcccgat
     1681 gcggctattt cgcgcagctc catttcctcc tgccatgcct tctgcctggc ataaaacagc
     1741 accgccgtca ttacccacaa gctcaatccg ccaagcaaaa accataccga attcagcatc
     1801 gcctgagacc cggtaagcag ccagacaatt atcgccgcaa caagcacagc agcctgtgtg
     1861 accgcgccca gtatcgcaac tttcctgcct cttgcctgtt ctttcatttc tacctcttat
     1921 attttttact cttttattat tttttcatgc tgccgcagac tttaatgcgt cttgtcggaa
     1981 acggataaca accaatggtt acgggtcata taaccaataa ttttcttctt aatgtcaaaa
     2041 ttcatccgtc gaaatcatat tcagcgcttt tacgtgtctg cgggttttgg gcagcatcaa
     2101 caacaaaaat accaatgcgc ttacgaggct caacgtaagg atgcccggcg tagactgtcc
     2161 catgaacagc cactgtgcat aagagcctgc ctgccacagc acaatgccgc gaaccagtaa
     2221 aattccgctc agtaacgtaa gcagccgcca cgaccatttt ctgcctctgc ccagcacgcg
     2281 agtgacgtac cacaacgtgc cgccgccgac aaacagcaaa ccaatcgtca caaaaccacc
     2341 cgtcgtatga ttcgccggca gtgtggccat tattacagcc ataatgacga atattatgcc
     2401 ggcgatacgt ccgaaccctg aaagaaggct aagagcagac ggcattgttg acgatgccgc
     2461 ctgtgcctcc atctgtcgcg tctgatacag cgcaatgccg acgaacaaca ccgcagccgt
     2521 ataacacgca cagtaacacg ccgccatgcc gacaaaactt accggtatgg gtttattact
     2581 cgtaagcgca tccagcggaa aaaacactgt catattcggc aataaccagc aaagaacatt
     2641 gataatcggc gcctccctgc cgggcccgac aaaatactcg ttcagccgcg cgtttatgag
     2701 cagataccat gaaccggcgg caagtatcaa aaaagttatc agaagcgtcg gcacctggcc
     2761 cagccgcgta cttgccgcag tcgcaaccgc tgtcaacaga acgatagcca aaaatatcag
     2821 aatcagcgca agtatcagcg acggttttat attgtcgtcg ctgaacgtat cggcgatatt
     2881 cacaaactgc cagtgaagat ctatgatgca cgacaaccca aacgccaccg tcatgaacac
     2941 aagcagcgcc atgcagcctg ttgcaataaa cgaccagttg aacatgtaat tccccaaacc
     3001 accaagaatc agggaaattg caaaagcgct cactccaaaa attatcaccg gccaattaat
     3061 cggcaccgat gcagccgaca cgaccttgtg cctcaccgtc agcagaaaaa caatcgacat
     3121 cagataatat gcaatcatta ccgccagcgt tattccggaa aattttccga gcacgaatgt
     3181 cgcgcgggct actggtttcg acgctactgt aagagcggtt ctgtcttcga tttccctgcc
     3241 gatagctgac gacgcactca acgctgccgt tataaatccc attatcagaa gcgtgctcaa
     3301 gcctacgttc tccatcatcc gttcatcgga tttttcgtaa tcagtgccca tcgtcatgcc
     3361 ggaaagcggc agtgacatga caagaatcgc gaacgtcaaa aatatcatca cgccgaaaat
     3421 cggctgacgt atcgtctgga ggaacgtatt tttcgctatg gcataaaatt ttatcattat
     3481 ctgattcgtt caactttggc gagctggtct tcgccggttt tttcatcaag atgcagatac
     3541 tcacccattc tcaccagccg cgccgagttg aaaatcacaa ccgtggcact gacggtatgc
     3601 aatatggctc cccagattgg cggaattttg cccagtcctg cgccaatcat gaaaaggatg
     3661 ataaagcaaa cgccgaaaag caggttctga tggacaacgc ggctggtggc gcgggagagt
     3721 tctataagga acggcagacg tcgaaggtcg gtattcatca gggcgatatt ggcggaattg
     3781 atagccacgt cgctgcctgc ggcgcccatg gcgatggaca gatcgcctgc cgccagtgcc
     3841 ggcgcgtcg
//
LOCUS       DAONFI010000224         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28873_length_3837_cov_3.162877, whole genome shotgun sequence.
ACCESSION   DAONFI010000224 DAONFI010000000
VERSION     DAONFI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28873_length_3837_cov_3.162877"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..566)
                     /locus_tag="PLK08_07515"
     CDS             complement(168..566)
                     /locus_tag="PLK08_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53383.1"
                     /translation="MKYGVVFAVLMSFSLQFICGCDDSPKEMLVDGNMVLFFDGLQKD
                     VKIIRQISEILPGGLTRIRTQFKNTDDDTKWIDIQVVWRDADGMELYKTSWQSFHLPG
                     RSVEQYDITSLKPAASYEFRIRKPDTKGKK"
     gene            complement(766..1875)
                     /locus_tag="PLK08_07520"
     CDS             complement(766..1875)
                     /locus_tag="PLK08_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPS53384.1"
                     /translation="MAVHEVALITGDGTGPELAAAAKKCIDAALAKTADAGSIKWTQC
                     VAGVDIMEQYGTPMPDATVDAVRNSDATLKAPITTPIGTGFRSVNVALRQTLDLYCCL
                     RPCKSYPGVRSRYENIDLVLVRENCEDLYAGVEFAKGLPETAELIEKINNISKEKKIK
                     TSRNTTGISIKSISVENTTRIVKFAFEYARKHGRKKVTFVEKANIMKHTDGLFKAVAE
                     EVAKSYPEIECEERLIDNMCMQLVQKPELYDVIVTENLYGDILSDLCAGLVGGLGVAP
                     GANIGDSGAVFEATHGSAPKYKGLNKVNPTAMILSGKLMLEYLGEEAAARRLENAIAK
                     VIAEGKDTTYDMKPDRNDPTAVGTSEMADAIIAAM"
     gene            2066..2680
                     /locus_tag="PLK08_07525"
     CDS             2066..2680
                     /locus_tag="PLK08_07525"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPS53385.1"
                     /translation="MTTISSKTIERFSIYRRLLERYAGERVASGVLAELAGVTAAQVR
                     RDMMALGCMGSSSRGYIAAELRQAIGEYLDAPEGLNIALVGAGELGRSILSYFAGRHK
                     KLRIVAAFDVDPWLTDRKLRGCECYQADKMPEIIAKKNIKLAIIAVPPAAAQQAADVL
                     IKAGVTGILNFSPTALTVPENIFVEYNDITTSLEKVAFFARHIS"
     gene            2704..>3837
                     /gene="hydE"
                     /locus_tag="PLK08_07530"
     CDS             2704..>3837
                     /gene="hydE"
                     /locus_tag="PLK08_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPS53386.1"
                     /translation="MDKDEIKNWLKESREDKLAELWSTADRVRRENVGDAVYLRGLVE
                     FSNHCARRCGYCGINATNSQVSRYRMTADEILECARQAEEYGYCTVVLQSGEDYGMPA
                     GWLADVIRRIKNRTGLAVTLSVGERSYDDLRLWKSAGADRYLLRFETSNRKLYDRIHP
                     PLPHQQKSDRFEILRRLREIGYEVGSGVMIGIPGQTWDDIAEDIVMFSRLDLDMIGVG
                     PYIPHPAGEVSGFAPAEPQMQVPNTEDVTYRVVALTRIMCPCTNIPSTTALATLNKNQ
                     GRELGLQRGANVVMPNVTPLKYRTLYEIYPEKACINETAQHCCRCLESRLASIGRTVG
                     RGSSSCSSTTGESAKAAWRSKTSSGVSRWLAPLATTMEFSPLAS"
BASE COUNT          928 a         1009 c          965 g          935 t
ORIGIN      
        1 cggttctaaa tctgaattat tccgctccac gtacggcatg ggtttatatt ccggcccaca
       61 gccgacaata aaagaaaaac atgccacaac ccaaaggcat aatatgcgcc tcatcaggtt
      121 cgcctccaaa tactggttaa ggtgcaatca aggcaacacg caaaaaacta tttcttgccc
      181 ttggtatcgg gctttcttat gcggaattcg taactcgcag ccggcttgag gctggtgatg
      241 tcatactgtt cgacagaacg gccaggcaga tgaaaagact gccagctggt tttgtatagt
      301 tccattccat ccgcatcgcg ccagacgacc tgtatgtcta tccatttcgt atcgtcgtcc
      361 gtatttttga attgcgtgcg tatacgggtc agcccacccg gaagtatttc cgaaatctga
      421 cggataatct tgacatcctt ctgaaggccg tcgaaaaaca acaccatgtt accatcgacc
      481 agcatttctt tgggagaatc atcgcagccg cagataaact gcaacgaaaa cgacataaga
      541 accgcaaaaa ccactccgta tttcatatta gccctttttg aataaacttt tcgtatgatt
      601 aagtataggc tgtaaactgt taaatgtaaa cccattttca caacacaatc acataatgag
      661 agcaggccgt ccgtaatgac gtttttttta aaaaaatgcc ataaaaaatg cgtcacgccg
      721 caaccgcgga atgacgcatt ttatttacct tcaacgcctt acatgttaca tggcggcaat
      781 aattgcatca gccatttcgc tcgtgcctac agctgtcggg tcattccggt caggcttcat
      841 gtcgtaggta gtgtctttgc cttcggcgat gactttggcg atggcgtttt cgaggcgtct
      901 ggcggcagcc tcttcgccga gatattccag cataagtttg ccggaaagaa tcatcgcggt
      961 gggattgacc ttgttcagcc ctttgtattt cggagcactg ccgtgggtgg cttcaaagac
     1021 tgcgccgctg tcgccaatat tcgctccagg tgcgacgccc agtccgccga caaggccggc
     1081 acataaatca ctcaaaatat ctccataaag attctccgtc acgattacgt cgtagagttc
     1141 cggcttctgg acgagctgca tgcacatgtt gtctatgagt cgttcttcgc actctatttc
     1201 cggatagctt tttgcaacct cttcagccac agccttgaac aggccgtccg tgtgcttcat
     1261 tatgttagcc ttctcgacga aggtgacctt cttcctgcca tgcttgcggg catattcaaa
     1321 cgcaaacttt actattcggg tggtattttc cacgctgatg gattttatgg aaatgccagt
     1381 cgtgtttctg gacgtcttga ttttcttttc cttgctgatg ttgttgattt tttctatcag
     1441 ttcagctgtt tccggcaggc ctttggcgaa ttccacgcct gcatagagat cttcgcaatt
     1501 ttcacgaacc aacaccaggt cgatgttttc atagcggctg cgcacgccag gatagctctt
     1561 gcacggacgc agacagcaat acaaatccaa cgtctgtcgc agtgcgacgt tcacgctgcg
     1621 gaaacccgtg ccgataggcg tggtaatcgg cgctttgagc gtggcgtcgg aattgcggac
     1681 ggcgtcgacg gtggcgtccg gcatcggcgt accatactgt tccatgatat ctacgccggc
     1741 gacgcactgc gtccatttta tactgcccgc gtcagccgtt ttggccaagg ctgcgtcaat
     1801 gcactttttg gcggcagcgg ctaattcagg gccggtaccg tcgccggtta tcaatgctac
     1861 ttcgtgtact gccatgatgt tatgcttcct ttcgtctgtg ttgtggcata ttcaaaacgg
     1921 gcggcatgtt atcagaaatc cggctgtacg tcaattttgc tcgaattttt ttgtatttca
     1981 ggttgattta ccacgccggc atggatacac tatcgtatgt gaaacatttc acagaattgc
     2041 atgtaatccg cacaaataaa gcaaaatgac cacaatttcg tcaaaaacca tagaaagatt
     2101 cagtatatat cggcggctgc tggaaagata tgcaggcgag cgtgtagcat ccggtgtttt
     2161 ggcggaactc gccggcgtta ccgcagccca ggttcgcagg gacatgatgg cgctaggctg
     2221 catggggtca agttcgcgcg gttatattgc cgcggaactg cgtcaagcca tcggagaata
     2281 cctcgacgcc cccgaaggat tgaacatcgc actcgtaggc gcaggcgaac tgggccgttc
     2341 catattgtca tattttgccg gccgacataa aaaattgcgg attgtagcgg cattcgacgt
     2401 tgacccgtgg ctgactgacc ggaagctgcg cggctgcgaa tgctatcaag ccgacaaaat
     2461 gcccgaaata attgcaaaaa aaaatataaa gctggccata atcgccgttc cgcctgccgc
     2521 cgcacaacag gcggcagacg tacttattaa agctggcgtt acaggaatac tcaatttttc
     2581 gcccacagcc ctgactgtgc ctgaaaatat ttttgtggaa tataacgaca taacgacatc
     2641 actggaaaaa gtggcattct ttgcaagaca tatttcttaa ctttttttta attcgattaa
     2701 ttgatggata aagatgagat aaaaaattgg ctgaaggaat cccgtgagga caagctcgcc
     2761 gagctttggt caactgccga ccgtgtacgg cgcgaaaatg tcggcgacgc agtatatctg
     2821 cgcgggctgg tggagttcag caatcattgc gccagacggt gcgggtattg cggcattaac
     2881 gcgacgaact cgcaggtaag ccgatatcgc atgacagccg acgaaattct tgaatgtgct
     2941 cgacaagccg aagaatacgg ctactgcacg gtggtgttgc agtcgggtga agattacggc
     3001 atgccagccg gctggctggc ggacgtcatc aggcgcataa aaaaccggac aggcctggcc
     3061 gtcacgctca gcgtaggcga gcgtagctac gatgacctgc gactctggaa atccgccggc
     3121 gcagaccgct acctcttgcg attcgaaaca tccaatcgca agctttacga ccgcattcat
     3181 ccgccgctgc cgcatcaaca gaaatccgac cgttttgaaa ttttgaggcg gttgcgggaa
     3241 atcggttacg aagtcggttc cggcgtgatg attggcattc ccggccagac atgggacgac
     3301 attgcagaag acatcgtcat gttcagccgc ctcgacctgg atatgatagg cgtgggcccg
     3361 tatattccgc atccagcggg cgaagtttcc ggcttcgcac cggcagaacc gcaaatgcaa
     3421 gtcccgaata ccgaagatgt cacataccgc gtggtagccc tgacgcgaat catgtgcccc
     3481 tgcacaaata ttccgtccac gacagccctc gccacgctca acaaaaatca aggccgcgag
     3541 ctgggcctgc aacgcggcgc aaacgtggtc atgccgaacg ttacgccctt aaaatacaga
     3601 acgctatacg agatttaccc cgaaaaagcc tgcataaacg aaaccgccca acactgctgc
     3661 cgctgccttg aatcgcgact tgcgtccata ggccgcaccg tcggcagggg cagctccagt
     3721 tgcagcagca ccacgggcga gtcggcgaag gcggcctggc gatcaaagac gtcgtcgggg
     3781 gtcagccggt ggttggcccc gctggcgacg acgatggagt tctctccctt ggcgtcg
//
LOCUS       DAONFI010000225         3830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_28945_length_3830_cov_3.837351, whole genome shotgun sequence.
ACCESSION   DAONFI010000225 DAONFI010000000
VERSION     DAONFI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28945_length_3830_cov_3.837351"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLK08_07535"
     CDS             complement(<1..308)
                     /locus_tag="PLK08_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPS53387.1"
                     /translation="MTLAVCEIFKSIQGESSRAGLPCIFVRLAGCNLNCSWCDTGYSR
                     TTDDATIMSLDDIVSQVEKLRCRRVEVTGGEPMLQKETPELLQWLCDAGYEVLIETNG
                     "
     gene            complement(305..1507)
                     /locus_tag="PLK08_07540"
     CDS             complement(305..1507)
                     /locus_tag="PLK08_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPS53388.1"
                     /translation="MNDMPQKFSSPFIRSPERSSTIFAVMTGAACLPLLGGIFFFGTH
                     AIVVSLLAVASCVFFERMYYRVTSLPSLLGKGHAYLTGVLLALTLPAFTPWWAVIMAG
                     GFAIIVGKAAFGGVGHYIWPPALIGRLSIAVLLPIIGMSAVVVPDDWPLLSQSDAILG
                     NISNAREISLQDYQGWKETTAADGSSGFLLEHPSAALFGLTDMADSSEPRYSSIGIVR
                     SDINNRKPALLATLPPLRDMLIGARPGGLGETSTLLIIAAGIYLIYRHYVKWQLSVAM
                     LAAAWLTAAVAPIRLCGPAGDEIVWYPLLAEGLEVGFTYVNYQLFSGELLLCAFILAA
                     DMTSRPVRTGGQFIFGALVGALAMLMKLYINTPIPAYIAVLIMCCFTPVIDSLWRPRV
                     LGARRSML"
     gene            complement(1504..2808)
                     /locus_tag="PLK08_07545"
     CDS             complement(1504..2808)
                     /locus_tag="PLK08_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPS53389.1"
                     /translation="MPARYGYFAGGIDLPEEKHATLDVPVRAAKIPQRLTLEFRRLCF
                     SQASPEVSIGQKVSAGQLLARVPEGQGLNLFSPAQATVAEITDGGIVLEEVERWEHPG
                     QPQGHMIWQALDMKRLRHEVLSSSLAIMRRGRVETLAGWLQRAIDYHCGHLVMNAMET
                     QPYVTSEHRTLVEFGRESLYGMVMLARAGVFNRMTLVVDSDRTDDYGHLLDTLTEFNI
                     EHVALPRKYPIGEDEILLSVLRKKEIPAGGNSCQTGASVIDPWTCMLLYRLIVCEQRL
                     GGRLVTLAFDKGCESGNYFVPFGMGVYDLLGPIRQPVIHGGPMNGRLCTPDDVVNYGT
                     DALLVIEMTSPERSTQCIRCGWCIDHCPARLNVSELNNAFETGNLARAKKLGVISCIN
                     CGVCSYVCPAKLPLKDRMGRLKSTINDMTNSLPLFHLREGRR"
     gene            complement(2821..>3830)
                     /locus_tag="PLK08_07550"
     CDS             complement(2821..>3830)
                     /locus_tag="PLK08_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPS53390.1"
                     /translation="GTATNPATTTTPAKAVTSPDDAMPVPAAVAATSGASEVEFTVEN
                     ARPIMANVNGKPIYMEKLTESLVSDYGLPIARQFVADEVVRQVLASRNISDKVTDQEI
                     NRESMRAMRMLFPMEDNITDEQFENILGQFLRNYRYTRRQWNQTMARNVLLSRLIEKN
                     IPVTDAELKDAFFRKYDGKYTARLIQVSSLDKAQGIIETLNDDKADFATLAKKESISP
                     TAGNGGLFEIGAKTSPDWVPPAIVKVVRSMKVGEVSEPVMAGATFHIIKLEAMTPPQD
                     VKFDDVKNELAALVREAKLQQERQKYLAELIKTADIDFVDPTLRTQNEKRLQKTDEAA
                     E"
BASE COUNT          888 a         1118 c          989 g          835 t
ORIGIN      
        1 ctgccgttgg tctcgattaa aacttcgtaa cccgcgtcgc acaaccattg cagaagttcg
       61 ggcgtctcct tctgaagcat aggctcgccg ccggtcactt caacacgccg gcagcggagt
      121 ttttcaacct gcgacacgat atcatcaagc gacattatcg tagcatcgtc ggtcgtacga
      181 gaataaccgg tatcgcacca cgaacagttc aggttacacc cggccagtcg cacgaaaatg
      241 cacggcaggc cggctcgcga gctttcgccc tgaatactct tgaaaatttc gcatacagcc
      301 agcgtcacag catgctcctt ctcgctccca aaacccgcgg ccgccagagt gaatctatca
      361 cgggcgtaaa acagcacatt atcaacacgg caatatacgc aggaatgggt gtatttatgt
      421 aaagtttcat cagcatggcc aacgcgccga caagtgcacc gaaaatgaac tggccgccgg
      481 ttctcaccgg tcgcgacgtc atatctgcgg cgaggatgaa cgcgcaaagc agcaactcgc
      541 cgctgaacag ttgatagttc acatacgtga atccaacttc cagtccttca gcgagaagcg
      601 gataccagac aatttcatcg ccggccggcc cgcacaggcg tattggtgcg acggctgccg
      661 tcagccatgc cgccgccaac atcgcgaccg acagctgcca tttaacgtaa tggcgatata
      721 tcagataaat gccggctgct ataatcagca gcgtcgaagt ttctcccagc ccacccggcc
      781 tggcgcctat gagcatgtcc cgcagcggcg gaagagtagc cagcaacgcc ggcttacggt
      841 tgttgatgtc gctgcgaaca atgccgatgg agctgtaccg cggctcagac gagtcggcca
      901 tgtccgtcag tccgaaaaga gccgccgacg gatgttccag caaaaaaccg ctcgaaccgt
      961 cagccgccgt cgtttccttc cagccctgat aatcctgcag cgaaatttcc cgcgcattac
     1021 ttatgttgcc gagtattgca tcggattggg aaagcagcgg ccagtcatcc ggcacgacga
     1081 cagccgacat gccgataatc ggcagcagta cggcaatcga aagtcggccg ataagcgccg
     1141 gcggccagat ataatggccc actccgccaa aggcggcttt gccgacgatt atggcgaaac
     1201 cgccggccat tatcaccgcc caccagggtg taaacgcagg cagcgtcaga gcgagcagca
     1261 cgcctgtgag atacgcatgc cccttgccga gcagcgacgg caaagacgtc acacgataat
     1321 acatccgctc gaaaaacacg cacgacgcaa cagccagcag cgaaacgaca atcgcatgcg
     1381 tgccgaagaa aaatatgcct cccagcagcg gcagacacgc cgcaccggtc atgacggcaa
     1441 aaatagtgct gctgcgttca ggtgaacgga tgaacggcga cgaaaatttc tgcggcatat
     1501 cattcatcga cggccctccc gcagatgaaa gagcggcagg gaattggtca tgtcgtttat
     1561 tgtactcttc aggcggccca tgcggtcttt gagcggcagt ttcgcaggac agacgtagga
     1621 acacacgccg cagtttatgc agcttatcac gccgagtttt ttcgccctgg cgagattgcc
     1681 cgtctcaaag gcgttgttaa gttcgctgac attcagccgc gcagggcagt ggtctatgca
     1741 ccacccgcaa cgtatgcact gtgtcgaacg ttccggcgag gtcatttcta tcacgagcag
     1801 cgcgtcagtt ccgtaattta cgacgtcgtc cggcgtgcac agtctgccgt tcatcggccc
     1861 gccgtgaatc acaggctgac gtatcgggcc gagcaggtcg tatacaccca tgccgaacgg
     1921 aacaaaataa ttgcccgact cgcagccttt gtcaaacgcc agcgtcacca gccgcccacc
     1981 taacctctgc tcacatacga taagccgata taacaacatg cacgtccagg gatctattac
     2041 ggatgctcca gtctggcagg aatttccgcc ggctggtatc tcttttttgc gaagcaccga
     2101 caggagaatt tcatcttcgc ctatcggata tttgcggggc agtgccacgt gttcgatatt
     2161 gaattccgtc aacgtgtcaa gcagatggcc gtaatcgtcc gtgcggtcgg aatccaccac
     2221 cagcgtcatg cgattaaaca cgccggcacg ggctaacatg accatgccgt agagcgactc
     2281 tctgccgaat tcgaccaggg ttcgatgttc ggacgtcaca tacggctgtg tttccatggc
     2341 gttcattaca agatggccgc agtggtaatc tatcgcccgc tgcagccagc cggctaacgt
     2401 ttcaacgcga ccgcgccgca ttatcgccag cgaggatgaa agaacttcgt gccgcaggcg
     2461 tttcatatcc aaagcctgcc atatcatatg gccctgcggc tggccgggat gctcccatct
     2521 ttccacctct tccagtacaa tcccaccgtc agttatttca gccaccgtcg cctgtgccgg
     2581 agaaaagaga ttcagaccct ggccttccgg cactctggcc aataattggc cggcagaaac
     2641 tttctgaccg attgaaacct ccggcgatgc ctggctgaaa cacagacggc ggaattccag
     2701 cgtcagacgt tgcggaattt tcgcagcacg aacgggcacg tcgagcgttg catgtttttc
     2761 ctccggcaga tctatgccgc cggcaaaata tccgtagcgt gcaggcattt tacgtgaata
     2821 ttactctgcg gcttcatcgg ttttctgcag ccgcttttca ttctgggtac gaagtgtcgg
     2881 gtcaacaaaa tctatatccg cggtcttaat cagttctgcc agatatttct gccgttcctg
     2941 ctgaagtttg gcttcgcgga cgagggctgc aagttcattt ttgacgtcat cgaatttgac
     3001 atcctgcgga ggcgtcattg cttccagttt gatgatatgg aaagtcgcgc cagccatcac
     3061 gggctcggac acttcgccga ctttcatcga acggacaact ttcactatag ccggcggaac
     3121 ccagtccggc gaagttttcg caccaatctc aaaaagtccg ccattgccag cggtaggact
     3181 tatggactcc tttttggcca gagtggcaaa atcagcctta tcgtcattca gcgtttcgat
     3241 tatgccctga gccttgtcga gcgaagaaac ctgtatcagc ctcgccgtat atttgccgtc
     3301 atatttgcgg aaaaacgcat ccttcagttc ggcatcggtc acgggaatgt tcttttcaat
     3361 caggcgggac agcagcacgt tacgagccat ggtctgattc cactgccggc gagtatagcg
     3421 ataatttctc aaaaactggc cgagtatgtt ttcaaactgt tcgtcggtga tgttgtcttc
     3481 catcgggaaa agcatccgca tggctcgcat gctttcacga tttatttcct ggtcagtcac
     3541 cttgtcggag atatttcggc tggccagcac ctgtcggacg acttcatcgg cgacgaactg
     3601 gcgggcaatc ggcaggccgt aatccgaaac cagcgactca gtgagttttt ccatatatat
     3661 gggcttgccg ttgacgttcg ccattatagg cctggcattt tcaaccgtga actcaacttc
     3721 cgaagcgccg gacgtggcag caacggcggc agggacaggc atagcatcat caggcgaagt
     3781 cacagcctta gccggcgtag tcgtcgtcgc aggatttgtc gcagttccac
//
LOCUS       DAONFI010000226         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29127_length_3814_cov_4.111466, whole genome shotgun sequence.
ACCESSION   DAONFI010000226 DAONFI010000000
VERSION     DAONFI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29127_length_3814_cov_4.111466"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLK08_07555"
     CDS             complement(<1..239)
                     /locus_tag="PLK08_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPS53391.1"
                     /translation="MLAASLPVSLIVIFLVVYGLIATEKMDKTIAAGLGAALVIGLHY
                     IPYEEALEKVDLNVIFLLIGMMMIVNILAKTGMFE"
     gene            complement(261..1184)
                     /locus_tag="PLK08_07560"
     CDS             complement(261..1184)
                     /locus_tag="PLK08_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPS53392.1"
                     /translation="MELTRYFSPEQVLLGLEVKDKWELLDKMVDSVLQGKICSRQDAD
                     VKNAIRQEVMNREKTTPTGFGGGFAFPHARVRGFNGFAFCIAVLKQPIDYNAMDNKPV
                     QIACMAITPENSPAIGLKAMGTVAVLLGDEAVRNVFLSAADPQKVFDLIDSKGLRLDS
                     SITAETIMREPYMLKIQRHTPLRQVAHQMFKHNVDACCVLDDEEHVVGEITCNALFKK
                     GVPDFFSQLASVAFIRDFDPFEAYFEHEAHSTAGDVMSEDFASLDVSATMMEIIFLLA
                     VKNYSKIYVTRNGRRAGIIDRIAVLEKVLNL"
     gene            1373..2689
                     /gene="hisD"
                     /locus_tag="PLK08_07565"
     CDS             1373..2689
                     /gene="hisD"
                     /locus_tag="PLK08_07565"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPS53393.1"
                     /translation="MNIQVIKNSEKDFRQKCRSVINLLRQGGLTSGPTAINIPTIVAD
                     IIARVASRGDDALIEYEKQLDRVTLTSQALRVPGDKIASVHRQAQPEFLSLMRRAIKN
                     IREYQQHIKISAPAPLRRGGRSLGVRYTPIERVGLYVPGGKALYPSTVLMTAVPAQVA
                     GVKEIVMVSPPNTDGDINPMVLALAGELGITEVYRLGGAQAIAALALGTQTIKPVQKI
                     VGPGNAFVAEAKRKLFGKVAIDSVAGPSEVLILADETADAKCIAADMMAQAEHDPGSA
                     ILITTSEPLCKTVIDELASQIKLLSRSDAIAASLEKYSAVILAADIDSACELANDFAA
                     EHLQIITADSHATLKKIRNAGAIFLGHYTPVPVGDYYAGPSHVLPTGGGAKCFSPLSV
                     NDFLKSSSIIEYDKKSLDEDKADIFDFASREGLTAHAKSPMKRFEK"
     gene            complement(2901..>3814)
                     /locus_tag="PLK08_07570"
     CDS             complement(2901..>3814)
                     /locus_tag="PLK08_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPS53394.1"
                     /translation="TRDVVLPILARSGLKVGRDFFLAFSPEREDPGRKDYSTKTIPKV
                     VGGYDRDSLAAAKACYQIAIDRVVPVSSCEAAEAAKILENTYRCVNIALVNELKVLFD
                     RMGIDIWEVINAAKTKPFGFTAFYPGPGLGGHCIPIDPFYLSWKAREYNMSTRFIELA
                     GEVNCAMPEYVVHRTMEALNLRKKALNGSKVLVMGLAYKKDVDDDRESPSYHLIELLM
                     ARGAKVDYNDPHCHEIQMTREHAELAGIKSKKLSPAMIASYDVVIISTDHTKYDYDMI
                     VKNAKLVMDTRNATANVKGSRKNIVKA"
BASE COUNT          974 a          987 c          920 g          933 t
ORIGIN      
        1 cattcgaaca tgccagtctt tgcaagtatg ttgactatca tcatcatgcc gataagcagg
       61 aagatgacgt tcaggtcgac tttttcgagt gcctcttcat atggtatgta atgcaggccg
      121 atgacgagag ccgcgccaag gccggcagca atggtcttat ccattttctc cgtcgcgata
      181 aggccgtaaa ccaccaggaa tattactatc agcgatacag gcagggatgc tgctaacatg
      241 atacacctct tttaaccttt ttaaaggtta agcacctttt ctagaactgc gatacggtca
      301 attatgcccg cgcgtctgcc gttgcgagta acgtaaatct ttgaatagtt tttcaccgca
      361 agcaggaaaa ttatttccat catggtggcg ctaacgtcca gagacgcaaa atcttcgctc
      421 atgacatcgc cagccgtcga atgagcctcg tgctcaaaat aagcttcaaa ggggtcgaaa
      481 tcgcgtatga acgccacgct ggccaactgt gaaaagaaat ccggcacacc cttcttgaaa
      541 agcgcattgc acgtaatctc gccgacaaca tgttcttcgt cgtcgagcac gcagcatgca
      601 tcgacgttat gcttgaacat ctggtgtgcg acctggcgaa gcggcgtatg cctttggatt
      661 ttcagcatat acggctcacg cataatcgtt tcagccgtta tggaactgtc caggcgaagg
      721 cccttggagt ctatgaggtc aaagactttc tgcgggtcgg cggcagaaag gaaaacgttt
      781 ctgacggctt catcgccgag cagtacagct acagtgccca tggcttttaa gcctatcgca
      841 ggagaatttt ccggcgttat tgccatgcat gctatctgca caggcttgtt gtccattgcg
      901 ttataatcaa tcggctgttt caggacggca atgcaaaaag caaatccgtt aaatccgcgg
      961 acacgggcgt ggggaaaagc gaaaccgccg ccaaagcccg tcggagtggt tttttcgcga
     1021 ttcatgactt cctggcgtat ggcatttttc acgtcagcat cctggcggct acagatttta
     1081 ccctgcagta cgctgtcgac cattttgtcc agtaactccc acttgtcttt cacttcaagg
     1141 cccaggagta cctgctcggg gctgaaatac cttgtcagtt ccatacttca cctatcgtaa
     1201 aagttcctgt ttttttatag acgcagaccg tcagcttaca tgtattgttt attacggtat
     1261 tttctatgcg caagagacgc agaaagaagc atgatacaaa tattttcaaa atagtcaatc
     1321 atgattgcaa aaaacgccgg catacttaaa atgtacgcag acggagaacg acatgaatat
     1381 ccaggtgata aaaaacagcg aaaaagattt cagacaaaaa tgtcggtccg taatcaatct
     1441 gcttcgtcag ggcggattga caagcgggcc gacggcgata aacatcccga caatagttgc
     1501 ggacatcatc gcccgggttg cttctcgcgg cgatgatgct ctgattgaat atgaaaagca
     1561 gcttgaccgc gtcacgctca cgtcacaagc acttagagtg cctggagata agatagcatc
     1621 cgtccatcgc caggcacagc cggaatttct ttcactgatg aggcgggcga taaaaaacat
     1681 acgtgaatat cagcaacata taaaaatatc cgcccctgcg ccgttacggc gaggcggccg
     1741 ttcgctcggc gttcgttata cgccgattga acgcgtcggc ttatatgtgc caggcggcaa
     1801 ggcgctctat ccgtcgacgg tactcatgac ggcagtgccg gcgcaggtag ccggcgtgaa
     1861 ggaaatcgtc atggtttcgc cgccgaatac ggacggcgat atcaatccaa tggtacttgc
     1921 attagccggc gaactaggca taaccgaagt ttatcgcctc ggcggagcac aagccatcgc
     1981 cgctctcgcc ctcggcactc agaccataaa accggtccag aaaatcgtcg gccccggcaa
     2041 tgccttcgtc gcagaagcca aacgcaaact attcggcaag gtcgcaatag attccgtcgc
     2101 cggcccaagt gaagtgctca tattagccga tgaaacggcc gacgcaaaat gcatagcggc
     2161 tgacatgatg gctcaggcgg aacacgaccc tggctcagcc atactcataa ccacgagtga
     2221 accgctctgc aagacagtca tcgacgaact cgccagccag attaagctac tctcacgcag
     2281 cgacgcgata gccgccagcc ttgaaaaata ctcagccgtc atattggcag ccgatattga
     2341 ctcggcctgc gaacttgcaa atgactttgc cgcggaacat ctgcagatta ttacggcgga
     2401 cagccatgcg acgctgaaaa aaatccgcaa tgccggcgca atatttttgg gccactacac
     2461 acccgtgccg gtgggcgatt attacgccgg cccaagccat gtcctgccga caggcggcgg
     2521 cgcaaaatgc ttcagcccac tgagcgtaaa cgattttctg aaatcgtcga gtatcatcga
     2581 gtacgacaaa aaatcactcg atgaagacaa agccgacatt tttgactttg cttcccgcga
     2641 aggccttacc gctcacgcca aaagcccaat gaaacgattt gaaaaataaa gcggccagcc
     2701 tcgacaatca aagcagaaat acctgttttt ttatatctct gccgaaaaat ctttcgctga
     2761 tattttcaaa atttttcggg aaatgtttta atttttgaaa caacgtcaaa aaaaaggcgt
     2821 cattccgtgt aaaaacggga aatgacgcct ttttataaat ttatcgttaa ctctgcactt
     2881 ttcgttcgga cgctgtttac ttacgccttg acgatattct tgcggctgcc tttgacatta
     2941 gcagttgcat tgcgcgtatc cataacgagc tttgcatttt tcacaatcat gtcgtaatca
     3001 tacttggtat ggtcggtact tattatgacg acatcgtatg atgcaatcat ggcgggcgaa
     3061 agttttttgg actttatgcc ggctaactca gcatgctcgc gtgtcatctg aatttcatgg
     3121 cagtgcgggt cgttgtaatc gaccttcgcc ccgcgtgcca tcagaagttc tatcaggtga
     3181 tatgaggggc tttccctgtc gtcgtcgacg tctttcttgt atgccaaccc cataaccagg
     3241 accttcgagc cgttgagggc ttttttacgc aggttcagag cctccatcgt acgatgaacg
     3301 acgtattccg gcatggcaca gttgacctcg ccggccagtt cgataaatcg cgtggacatg
     3361 ttatattcgc gggctttcca cgacagatag aacgggtcta tcggtatgca gtgaccgccg
     3421 aggccgggcc ctggataaaa cgcggtaaaa ccgaacggtt tggtcttggc cgcgtttatc
     3481 acttcccata tatcaatacc catgcggtcg aacagaactt tcagttcgtt tacaagtgcg
     3541 atattcacgc acctgtaggt attttcgaga attttggcgg cttcggcggc ttcgcaactg
     3601 ctgaccggca caaccctgtc gatggcaatc tggtaacacg ccttggctgc agccagcgaa
     3661 tcacggtcat aaccgccaac tacctttggt atggttttgg tagaataatc ttttctgcca
     3721 ggatcttcgc gttcagggct gaacgccagg aagaaatccc tgccgacctt aaggccggat
     3781 ctggcgagta tcggcaatac gacgtcgcgg gtcg
//
LOCUS       DAONFI010000227         3806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29210_length_3806_cov_4.219941, whole genome shotgun sequence.
ACCESSION   DAONFI010000227 DAONFI010000000
VERSION     DAONFI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29210_length_3806_cov_4.219941"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLK08_07575"
     CDS             complement(<1..210)
                     /locus_tag="PLK08_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPS53395.1"
                     /translation="MMRTLTGQEVRQAVRGRWLSMNNHVPITGVSIDSRKVKSGDLFI
                     AIKGDHFDGHDFLQTAAENGGVCAIL"
     gene            complement(207..1682)
                     /locus_tag="PLK08_07580"
     CDS             complement(207..1682)
                     /locus_tag="PLK08_07580"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPS53396.1"
                     /translation="MRFSTLLEEAGLKPKACVGDADITTICANSRKCSEKPGTLFVAI
                     DGTSVNGHKFIPEACASGAAAVVCRDSAGVPSGMPYAIVDDTREAAGLLAQAIYGWPA
                     RKLTCIAITGTNGKSTTAHIIFKTLNDMGIPAGMLGTITYETGARSIQARMTSPDAIY
                     LAEMMAEMVQSGLTHMVMETSSHAIDQKRIAGLQFQAAVFTNLTGDHLDYHLTMEEYF
                     NAKAKLFENLSPDAFAVINRDDKYSEQLAQRTRAKILWYGLNPLADIRGLIKTIDADG
                     TNFELTANDRTTDVHTPLIGRHNVFNILAAIGAIQAIGIDPQHSIEKIASVRNIPGRL
                     QRIETQADYKVFVDYAHTDDALANVLSALRPITPKRIIVVFGCGGDRDKTKRPRMAKV
                     AQEYADVIVVTSDNPRSEEPLDIIRDITAGFDQGHLSRVQIYPDRKQAIEQAINFADN
                     GDIVLIAGKGHENYQIIKGLRNHFDDVEVAGAAVNMREKEQ"
     gene            complement(1838..3655)
                     /locus_tag="PLK08_07585"
     CDS             complement(1838..3655)
                     /locus_tag="PLK08_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPS53397.1"
                     /translation="MNLGDKIRYCIIFIALLGGLAFVGIKQGLMIDAKLHPKTPQQAD
                     ISQMVDDASQGQRIVMPLPARPGSIYATSRQAWTLAAASRQVPSCFVDPKYLREKYDE
                     HDIRQLAQNIADILNSDPAAVYEIMTAQSQKRFVWIAREIEAVQADKIRELNNRALGI
                     SYEWRREYPNGPLAGSVIGFRCKDGSPGVGLEAYLDDIVKASDGKRIVLGDAGRRGIY
                     TDIDATKLPQDGGNVFITIDLVIQDILQRVVSDTVSEYKAVWGTGIVLDPWTGEILAM
                     CATPSFDPNTYNDTPIANMTNHAITSPYEPGSVFKPIIAAAAVNTGVINYQTKIFCEN
                     GVYHASKGGRISDHGNSYGYITVETGIIKSSNIMMAKIGEKLGNKKLFSLLHDWGFGQ
                     DTGIDLPQESPGIVRKLPKWDGYSTRRVPFGQEIGATSIQLAMAFASFANGGELMKPQ
                     IVGMVTDPRGKVLYKAKPKVVRRVLSQRVAKETLQALRLVVTEGTGKKAKSDFYTIWG
                     KTGTAQVPGPHGYMEGAYTASFIGGAPFENPRVICLISIYRPDRYKGYYGGTVTAPAV
                     KSVLEQTLEYLNVPHDLAPKADAIDEQARQNRLGDDMRD"
BASE COUNT          718 a          991 c         1107 g          990 t
ORIGIN      
        1 taatattgcg cagactccgc cgttttcggc ggctgtttgc agaaaatcat ggccgtcgaa
       61 atggtcgccc ttgatggcga taaataaatc gccggatttg acttttcggg aatctatcga
      121 cacgccggtt attggtacgt ggttattcat gctcagccag cggccgcgga cggcttggcg
      181 gacttcctga cctgtgagag ttcgcatcat tgttcttttt ctcgcatgtt tactgcagcc
      241 ccggccactt caacgtcatc gaagtgattc cgcaggcctt tgataatctg gtaattctcg
      301 tggcctttgc cggcgatgag aacaatatcg ccattatccg caaaattgat tgcctgttca
      361 atagcctgct ttctgtcggg ataaatctgt acgcggctga gatggccctg gtcgaaaccg
      421 gctgtgatgt cgcggatgat atccagcggt tcttcgcttc tggggttgtc gctggttacg
      481 acgatgacgt cggcgtattc ctgagccact ttcgccatgc gggggcgttt ggtcttgtcc
      541 ctgtcgccgc cgcagccgaa aaccacgatt atccgcttgg gcgtgatggg ccgcaacgcg
      601 ctgaggacat tcgccagcgc gtcgtcggta tgggcgtaat ccacgaatac cttgtagtcc
      661 gcctgtgttt cgattcgctg aagacggccc ggaatgttac gcaccgatgc gattttctct
      721 atcgaatgct gcgggtctat gccgatggcc tgaatcgcac cgatggcagc aagaatgttg
      781 aacacgttat gccggccaat cagcggagtg tgaacgtcag tagtgcggtc gttcgccgtc
      841 agttcgaaat tagtgccgtc cgcgtctatg gttttaatca ggccgcgtat gtcggctaac
      901 ggattgaggc cgtaccataa aattttcgcc cgcgtgcgtt gtgcgagctg ttcgctgtat
      961 ttgtcgtcac ggttgattac ggcgaatgcg tcgggcgaga gattttcaaa aagctttgcc
     1021 ttggcgttga aatattcttc catcgtcagg tgataatcga gatggtcacc ggtcagattt
     1081 gtaaagacgg cagcctgaaa ttgcaggccg gcgatgcgtt tctggtctat cgcatggctg
     1141 gacgtctcca tgaccatgtg cgtcagtccc gactgcacca tttcagccat catttccgca
     1201 agatagatgg cgtccggcga ggtcattctc gcctgtatgc tgcgggctcc ggtttcgtac
     1261 gttatcgttc ccagcatgcc ggcgggtatg cccatgtcgt tgagcgtttt gaagatgata
     1321 tgggctgtag tgcttttgcc gtttgtgccg gtaatggcga tgcaggtgag ctttctggcc
     1381 ggccagccgt atatcgcctg tgccagcagg ccggctgcct cgcgggtatc atccacgatt
     1441 gcataaggca tgccggaagg aacgccggct gaatccctgc acactacggc ggctgcgccg
     1501 cttgcgcatg cctcaggaat gaacttgtgg ccgttgacgc ttgtgccgtc gattgctacg
     1561 aaaagagtgc ccggtttttc agagcatttg cgactgtttg cgcagatggt tgtgatgtcg
     1621 gcgtcgccga cgcaggcttt gggtttcagt cccgcttctt ccaggagtgt cgaaaaccgc
     1681 atgatccgcc atttcttgtc tcaggtatat ttatcgttca tccggcgtcg tgaatggccg
     1741 gtcagtaatc cgctgcccgg gataatctgc atgacgcatg cagacagagc ccgggccaac
     1801 gctgggaatc ggttcgcatg tataaaagac gtacaggtca gtcccgcata tcatcgccca
     1861 gccgattctg cctggcctgt tcgtcaatcg catcagcctt gggtgcaagg tcgtgcggta
     1921 cattcaaata ttcgagtgtc tgttcaagaa cgctttttac ggcaggggcg gtcacagtac
     1981 cgccataata tcctttatat cggtcaggcc tgtatattga gattagacaa attaccctgg
     2041 gattttcaaa aggagcgccg ccgatgaagc tggcagtgta cgcaccttcc atatatccat
     2101 gcgggccagg gacttgcgcc gtgccggtct tgccccaaat tgtgtaaaaa tcgcttttgg
     2161 cttttttgcc tgtgccttcg gttacgacca gccgcaatgc ctggagggtt tctttggcga
     2221 cgcgctggct gaggacgcga cggacgactt tgggtttagc cttgtacagt accttgccgc
     2281 gagggtcggt aaccatgccg actatctgcg gtttcatgag ctctccgccg ttggcgaacg
     2341 atgcgaatgc catcgcaagc tggattgacg tcgcgccgat ttcctggccg aacggcacac
     2401 gtcgcgtgga atagccgtcc catttcggca gtttacgcac gatgccaggc gactcctgcg
     2461 gcagatctat gccggtatcc tggccgaaac cccagtcgtg cagcagactg aacagtttct
     2521 tattgcccag cttttcgccg attttagcca tcatgatgtt gctggatttg atgatgccgg
     2581 tctcaaccgt gatatatccg taactgttgc cgtggtctga aattctgccg cccttggatg
     2641 cgtgatatac gccgttttca caaaaaatct tcgtctggta atttattacg cccgtgttca
     2701 ccgcggcggc ggctatgatg ggtttaaaca cgctgccggg ctcatatgga ctggttatgg
     2761 cgtgattggt catgttcgcg atgggggtgt cgttgtacgt gtttggatcg aacgacggcg
     2821 tagcgcacat cgcgagtatt tcgccggtcc aggggtcaag aactatgccg gtgccccaga
     2881 cggctttata ttcgctgact gtgtcgctga cgacgcgctg gagaatgtcc tgaatgacga
     2941 ggtctatggt gatgaaaacg ttgccgccgt cctgcgggag tttagtggcg tcgatgtcgg
     3001 tatatatgcc ccgtcggccg gcgtcgccca gaactatgcg tttgccgtcg gatgccttga
     3061 cgatatcgtc cagatacgct tcaaggccta cgcccggcga gccgtccttg caacggaagc
     3121 ctatcaccga ccccgctagc gggccattgg gatattcccg ccgccattcg taggatatgc
     3181 ccaaggcgcg attgttgagt tccctgattt tatcagcctg cacagcctcg atttcgcgtg
     3241 caatccagac gaatcttttt tgggactgag cggtcatgat ttcgtagacg gcggctgggt
     3301 cggaattgag aatgtcggcg atgttctgtg caagctggcg gatgtcatgc tcgtcgtatt
     3361 tttcccgcag atatttcggg tcgacgaaac agcttgggac ctgtcggctg gctgcggcga
     3421 gcgtccaggc ctgacggctg gtggcgtaga tgctgcccgg ccgagccggc agcggcatga
     3481 cgatacgctg gccttgcgag gcgtcgtcga ccatctgcga aatatccgcc tgctgcggag
     3541 ttttggggtg aagtttggcg tctatcatca ggccctgctt tatgccgaca aacgccagcc
     3601 cgcccaacag cgcaatgaaa attatgcaat atcggatttt atcgccgagg ttcatttaag
     3661 ttccgttgat tatggccgga tgatgacggc atggatgtcg gcgtcgcggc ggaagagtct
     3721 tccacgactt tagcgttaga aatctggatg aggttcagtg ccattgcgtc ggctctgaat
     3781 cgtatcgacg caggcgtaag gaggtg
//
LOCUS       DAONFI010000228         3795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29326_length_3795_cov_4.318717, whole genome shotgun sequence.
ACCESSION   DAONFI010000228 DAONFI010000000
VERSION     DAONFI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29326_length_3795_cov_4.318717"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLK08_07590"
     CDS             complement(<1..531)
                     /locus_tag="PLK08_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPS53398.1"
                     /translation="MKWFSGLEKIVERDVPLAKYTWFRLGGPAEFFVRPQNVDELQDV
                     VCRCRENSVPIYVLGCGANLLVADSGVKGVVIYLRQGDFMDMNFSTPEHAVIGAGAEM
                     GKVVVQCVRKGLSGLEALTGIPGSIGGCIRMNAGGAFGDIGSAVENVTVMTGEGEIFK
                     RTRADLTFGYRSTNIVA"
     gene            complement(497..1924)
                     /gene="murC"
                     /locus_tag="PLK08_07595"
     CDS             complement(497..1924)
                     /gene="murC"
                     /locus_tag="PLK08_07595"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPS53399.1"
                     /translation="MLKTGSQSDAAMPQGEFAGRHIHFIGISGSGMRALAAMLMRHGA
                     IVSGSDRSESETVANLRSLGATVYIGQCGENISDDCDIVVYSAAIKDQNPEFLAACEK
                     GCEVLKYAAMLGRMMKEKTGIAIAGTHGKSTTTAMVAYILRQGGVDPSFVVGANVQQL
                     GGPSDVGGGEHFVAEACEFDSSFLHLSPKYATILNIEEDHLDYYRDIDAIIEAFRAFA
                     DRVKPGGVIIANGEDRNVRTALSDAKCKVETFGMNGDSFWRGVITNPDRRKIHMEILR
                     DGKPFGELLVPLAGLHNAYNCLAATALLYNAGLDAETILRNMETFRGACRRMTYKGCG
                     RGITVVDDFAHHPTEIQVTLRALRDFYQPKRLICVFQPHQHSRTRFLLKDFAQSFALA
                     EEVIVPDIYFVRDSEQERDYVSSKDLVAQIRLRGGVAVYFKTFELIVEYLKGICENGD
                     LVVTMGAGNVWEIANEMVQWLGKDS"
     gene            2243..3469
                     /locus_tag="PLK08_07600"
     CDS             2243..3469
                     /locus_tag="PLK08_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPS53400.1"
                     /translation="MSDFRIKEFANGLVLLGLEKPEFESVAMCISLCSGSAYDPQSLA
                     GAASVGSEWLFRGAAGLNSRQLNDALDSLGCQHAESVSGESIAFSASMLGCNLDKIFD
                     IYAGILTQSTLADESFDPCRMLVLQDLQSLEDEPSRKCTALLKEKFFPYPLGRLATGN
                     TETLSAMTPAAAREHLRKCLSPRGTIIAVAGNFDFDGFAARVEEKLGGWNTQALPDVK
                     LTAPAGGITHIEKDTAQMHISLAYKSVPVEDDMYYAARTGVAVLSMGMGSRLFTEVRE
                     KRGLVYHVSARYSSLRNYAGIFAYAGTRPEMAQQTYDVMTGELKRLVEGITDDELQIA
                     NTQLRSVLIMQSQSTIALAGMMAADWYHLRRLRTLDEISQKTLAVTCHDILQYLQKYP
                     ADNFTVFTIGPKSLDI"
     gene            3487..>3795
                     /locus_tag="PLK08_07605"
     CDS             3487..>3795
                     /locus_tag="PLK08_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPS53401.1"
                     /translation="MEFRTAKLSNGLTIIAELNPTAASLALGFFTRTGSRDETPEISG
                     VSHFREHMVFKGTDRRDNFKVNLDFDRMGAQYNAFTSEENTVFYGSVLPEFQDDLLDL
                     L"
BASE COUNT          894 a         1100 c          941 g          860 t
ORIGIN      
        1 cgccacgata ttcgtgcttc gatacccgaa ggtcaggtca gcgcgggttc gtttgaatat
       61 ttcgccttcg ccggtcatga cggtgacgtt ttccaccgca ctgccgatat cgccgaacgc
      121 accgccggcg ttcatgcgaa tgcagccgcc tatgctgccc ggaatgccgg tcagcgcctc
      181 aaggccgctt aaacctttgc gcacgcactg aaccaccact ttgcccatct cggcgcctgc
      241 gccgattact gcgtgttcag gcgtactgaa gttcatgtcc atgaagtcgc cctgacgcag
      301 ataaatcaca acgcctttta cgccggaatc ggctacgagc agattcgccc cgcagccgag
      361 aacgtaaatc ggcacgctgt tttcgcgaca acggcagacg acgtcctgaa gttcgtccac
      421 attctgaggt ctgacgaaaa attccgccgg cccgcccagc cgaaaccagg tgtatttcgc
      481 aagcggaacg tcacgctcaa ctatcttttc caagccactg aaccatttca tttgcaattt
      541 cccatacgtt gccggcgccc atcgtcacca cgaggtcgcc gttttcacat atacctttta
      601 gatattctac aataagttca aacgttttaa aatagaccgc cacgccgccg cgaagacgaa
      661 tctgggcgac gagatccttg ctcgatacgt aatcgcgttc ctgttcgctg tcgcgtacga
      721 aataaatatc gggcactatc acttcttcag ccagcgcgaa gctttgtgcg aaatccttta
      781 gcagaaatcg cgtacggctg tgctggtgcg gctggaagac gcatatcagc cgcttgggct
      841 gataaaaatc ccgcaacgca cgcagtgtaa cctgtatttc cgtggggtga tgcgcaaaat
      901 catccaccac cgtaatgccc cggccgcagc ctttgtatgt catgcggcgg catgcgccgc
      961 ggaacgtctc catatttcta agtatcgttt cggcatcaag gccggcgtta tacagaagcg
     1021 cggtggcggc aagacagtta taggcgttat gcaatccggc tagcggcacg agcagctcgc
     1081 cgaacggctt gccgtcacgg agtatttcca tgtgaatctt gcgacggtcg ggattggtta
     1141 tcacgccgcg ccagaaagaa tcgccattca tgccgaaagt ttccactttg catttcgcat
     1201 cggacagggc agtcctgacg ttcctgtctt cgccgttggc aattattacg ccgccgggct
     1261 tgacccggtc ggcaaaagcg cggaaggcct ctattatggc gtcaatgtcg cgataataat
     1321 ccagatggtc ttcctcgatg ttcaatattg tagcatattt tggcgaaagg tgaagaaaac
     1381 ttgagtcaaa ttcgcaggct tcagccacaa aatgttcgcc gccgcccaca tcgctcggcc
     1441 cgccaagctg ctggacgtta gccccgacca caaaactggg gtctacgccg ccctgacgca
     1501 atatatatgc aaccatcgca gtagtagtgc ttttgccgtg agtgccggcg atggcgatgc
     1561 cggttttttc cttcatcatg cggccaagca tggcggcata tttcaaaacc tcgcaacctt
     1621 tttcgcacgc cgcgagaaat tccggattct gatccttgat ggcggcggaa tagactacta
     1681 tgtcgcagtc gtcagaaata ttttcgccgc attggccgat gtagacggtc gcgccgagcg
     1741 aacggagatt cgccaccgtt tcactctcgc ttctgtcact gccgctgaca atcgcgccgt
     1801 gacgcatcag catggctgcc agtgcacgca tgccggaccc gcttatgcct ataaagtgaa
     1861 tatgcctgcc tgcaaattca ccctgcggca tagccgcatc agattgactc ccagttttaa
     1921 gcatccgttc tccaagattc tatccataat aatccggcac acttacaagc ttttataaaa
     1981 atacgacaaa agtgtgcctt ttctattatc ggcaatacgg cggcggagtg ttaatgaaaa
     2041 agttttttga cgccgttctt aaaattttat gcaaaacaag aataaaagtg actcttatag
     2101 ctgttcccca aaagcctttt ccttttggac tagctataaa aaattccgca actgttttgc
     2161 ctcttgctct tgggtggcaa aatcgttacg ttagagcggt tttgagtgtt ttttcgcaaa
     2221 gattttcgca aaggttttta cgatgtcaga tttccgaata aaagagtttg ccaacgggct
     2281 ggtactgctc ggccttgaga aaccggagtt cgaatccgtt gccatgtgca tatccctttg
     2341 ttcgggcagt gcttatgacc cgcagagcct ggctggcgca gctagcgtcg gcagtgaatg
     2401 gctgttccgc ggggcagccg gcttgaatag tcgccaactc aacgacgccc tcgattcgtt
     2461 gggctgccag cacgccgaat ccgtctctgg cgaatccatc gccttcagcg caagcatgct
     2521 gggctgcaat ctggacaaaa ttttcgacat ttacgccggc atacttacgc agagcacgct
     2581 tgccgatgaa tcgttcgacc cctgccggat gctcgtgctt caggaccttc aaagtctcga
     2641 agatgagccg tcccgcaaat gtaccgctct gctgaaggaa aaatttttcc cgtatccgct
     2701 gggccggctt gccacgggca acacggaaac gctttccgcc atgacgccgg ctgccgcacg
     2761 cgaacacctg cgaaaatgtc tttcccccag gggtactata atagcggtcg ccggcaattt
     2821 cgacttcgac ggattcgccg ccagggttga agaaaaactc ggcggctgga atacgcaggc
     2881 tctgccggac gtcaagctca ctgcgccagc cggcggcata acgcacatag aaaaagacac
     2941 cgcccagatg cacatctcgc tggcgtacaa atccgtgccc gtggaagacg acatgtatta
     3001 cgccgcccgc accggcgtag ccgtcctgtc tatgggcatg ggcagccggc tgtttaccga
     3061 agtccgtgaa aaacgtggcc tggtctatca cgtctcggca agatactcaa gcctgcgcaa
     3121 ctacgccggc atattcgcgt acgcaggcac aaggcctgaa atggcccagc agacttacga
     3181 cgtaatgacc ggcgaactga agcggctggt cgaaggcata accgacgatg aactgcaaat
     3241 cgccaacacg caactgcgaa gcgtcctcat catgcaaagc cagtccacca tcgccctggc
     3301 cggcatgatg gctgccgact ggtatcacct ccgccgcctg cgcacgctgg acgagatttc
     3361 gcaaaaaact ctcgccgtaa cctgccacga catactgcaa tatctccaaa aatatccagc
     3421 cgacaatttt acagtattca caatcggccc caaatcgctc gacatctgat aatacggatt
     3481 ttaaaaatgg aattcagaac cgcaaaatta agcaacggac ttacgataat cgccgaactc
     3541 aaccctacgg cggcatcgct ggcgctggga tttttcaccc gcaccggcag ccgcgatgaa
     3601 acgcccgaaa tatcaggcgt aagccatttt cgggaacaca tggtattcaa gggcacggac
     3661 aggcgcgaca attttaaggt caacctggac ttcgaccgca tgggcgcaca gtacaacgcc
     3721 ttcaccagcg aggagaacac cgtcttctac ggcagcgttt tgcctgaatt ccaggacgac
     3781 ctgcttgacc tgttg
//
LOCUS       DAONFI010000229         3790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29374_length_3790_cov_6.512985, whole genome shotgun sequence.
ACCESSION   DAONFI010000229 DAONFI010000000
VERSION     DAONFI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29374_length_3790_cov_6.512985"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2094
                     /locus_tag="PLK08_07610"
     CDS             <1..2094
                     /locus_tag="PLK08_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53402.1"
                     /translation="MSKTNSIRAGKAFVEVFTDNSKLVSGLRAAESKVKAFGGKIRNI
                     GAALTGLSAAILTPLAGAMKVFSSYGDEVAKMSKRTGVSVETLSELSFVASQSGTEFA
                     TLENAFRKMQKSIYDAERGLSTAVDGLADLGLKFEDLKNLSPEQQFKLMADRMSQIKD
                     PTKAAGVAMAIFGRTGTNLLPMFQQGAKGIEELQQKARELGLTMSGEDAKAAEDFTDA
                     MDSVWKQVKMVTFQVGAALGPTVTDLSGIFKRCLKTIIDWCKANKELIKTVAKVALIV
                     GGVGIAIMTLGVSIQGLGFAIGGLATVAKIVITSMAMVKVAIAALLTPIGLTLTAITG
                     LTIGVLIFTGQGAAALKFLSNAFMTLKDEAVKTFGAIGKAVAAGDLGLAAEVLWLSIK
                     LQFQKGKTEIAKIWSDFTFGLSAAFTQVKFDVCKIWAGLTAELKKLWADFTFGCQALF
                     YGLKYEISNIWLGLVNVLKNAWDGFMKWHATTVNTLSNKLAKAMLHIQGLFDKDFDVE
                     FAVKQVDSMTSDRQKEIDDEFSKKKKANDKEYADSVAENKTARDKKFTELATDNQDKL
                     QKATDEYNKALADLKKARDERFVDLTTKNQTEISEAEQALEKARSAWQSAVDRSNELS
                     KSGEGSKLSEFDPGEIADSISKATASVRGSFQHVRGFGGGSSAADRTAVATEQTAKNT
                     SKILKEQKKSDLKFT"
     gene            2134..2943
                     /locus_tag="PLK08_07615"
     CDS             2134..2943
                     /locus_tag="PLK08_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53403.1"
                     /translation="MSDTVTCEEKFDSRTRIYGESAELLYVVRGTDDDDVVFEKILET
                     SPEDYKGMLRGHPEIEPLGGGIWIGTVGYYAASLEVEDPFNTVMNFDTSGGTHHITQS
                     IKTVGRYPANAPNYKGAIGYDGKNVNGVDILAPALNLSETHDFAASMITMGYIANLSR
                     KTGMYNSDLFRGFEPGEVLFAGASGTKTEDTINPIWEVTFNFMVSRNRKNFRVGDIQV
                     SLKRGWDYMWVRYEDAVDDAAGDVIKAPAAVYIEQVYESTSFASLGINRAL"
     gene            complement(2951..3424)
                     /locus_tag="PLK08_07620"
     CDS             complement(2951..3424)
                     /locus_tag="PLK08_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS53404.1"
                     /translation="MAEFLKAFKAEITKVARKELKNLTGDMKKDIVKLKKTVATLRKE
                     VNDLSRQKRQIEREVVRNSELRQQEEEKQLKVNVDKIRVTGTMIRKLRTKLKLSQADM
                     GVLLGVTGQSIYQWERRENESLKRIRNAAKIELAKLRKMTRGEVKTLLEQFQTEE"
BASE COUNT          989 a          889 c         1002 g          910 t
ORIGIN      
        1 atgtctaaaa caaattccat tcgcgccggg aaggcgttcg tagaggtttt taccgacaat
       61 tccaaactgg tgagcggtct gcgtgcagct gagtcgaagg taaaggcgtt cggcggcaaa
      121 atccgcaaca tcggcgcggc cctgaccggc ctgtcggcgg cgatacttac tccgctggca
      181 ggtgcgatga aggttttttc cagctacggt gacgaagtcg ccaaaatgtc caagcgtaca
      241 ggcgtatccg tcgaaacgtt atccgaactg agcttcgtag cttcccagag cggtaccgag
      301 ttcgccacgc tcgaaaacgc attccgcaag atgcagaagt caatctatga cgcggaacgc
      361 ggattgtcta ccgcggttga cggtctggct gacctcggcc tgaaattcga ggacttgaag
      421 aatctttcac ccgaacagca gttcaagctg atggccgatc gcatgagcca gataaaagac
      481 ccgaccaagg cggccggcgt cgcgatggcc atcttcggtc gaaccggaac gaaccttctg
      541 cctatgtttc agcagggagc gaagggtatt gaagagcttc agcagaaagc ccgcgaactc
      601 gggcttacca tgagcggtga agatgcaaag gcggctgagg attttaccga cgcgatggat
      661 tccgtctgga aacaggtcaa gatggttacg ttccaggtcg gcgccgctct cggacctacc
      721 gttacggact tgtccggcat tttcaaaaga tgcctcaaga cgattatcga ctggtgtaag
      781 gccaataaag agttgataaa aactgtcgcc aaggttgctc tgatagtcgg cggcgtcggt
      841 atcgcaataa tgacgcttgg cgtgagtatt cagggattgg ggtttgccat cggcggtctg
      901 gctactgtgg cgaagatagt tatcacgtcc atggcgatgg taaaagttgc aatcgccgct
      961 ctgcttactc cgataggctt gacgcttacc gcaattacag gattgacgat aggcgttctg
     1021 atatttaccg gtcaaggcgc agctgcgctg aagtttttgt cgaatgcgtt catgacgctc
     1081 aaagacgagg cggtaaaaac gttcggggcc ataggcaagg ccgtcgcggc gggagatttg
     1141 ggtttggctg ccgaagtgtt gtggctgagc ataaaactcc agttccagaa aggcaagacc
     1201 gagatagcca agatatggag cgactttact ttcggcctgt cggcagcgtt cacgcaggta
     1261 aagtttgacg tgtgcaagat atgggcgggg ctgactgctg aactcaaaaa actgtgggct
     1321 gattttacgt tcggctgcca agctcttttc tacgggctca agtatgagat aagcaatatc
     1381 tggttagggt tggtgaacgt cctcaaaaat gcctgggacg gttttatgaa atggcacgcc
     1441 actaccgtaa atacgctgtc aaacaagctc gccaaagcca tgcttcatat tcaaggacta
     1501 tttgacaaag atttcgacgt cgaatttgcg gtaaagcagg tcgattccat gacgtcagac
     1561 cgccagaagg aaatcgacga cgagttttcc aaaaagaaaa aggccaatga caaggagtac
     1621 gcagacagcg tagctgaaaa caaaactgcc cgcgacaaga agtttacgga acttgcaacc
     1681 gacaaccagg acaagcttca gaaggcgact gacgaataca acaaagcctt ggctgatttg
     1741 aagaaagcgc gtgacgaacg tttcgtcgat cttaccacca agaaccagac tgaaatatcc
     1801 gaagccgagc aggcactcga aaaagctcgc agcgcatggc aaagtgccgt agacaggtcc
     1861 aacgaactca gcaaatccgg cgaaggcagc aagctctcgg aatttgaccc tggtgagata
     1921 gccgattcca taagcaaggc caccgccagc gtgcgcggga gtttccagca tgtacggggt
     1981 ttcggcggag gcagttctgc agccgacagg acggcggtcg ctacggagca gaccgcgaaa
     2041 aacacttcaa aaatcctcaa ggaacagaaa aagtctgatc tgaaattcac ctgattcttt
     2101 tttgtttttt taaaaagagg aataaggata atcatgagcg atactgtgac gtgcgaggag
     2161 aaattcgatt cccgcacgag gatttacggc gaatcggcgg aactgcttta tgtagtacgc
     2221 ggtactgatg acgacgacgt cgtttttgaa aaaatcctcg aaacctcgcc tgaagattac
     2281 aagggcatgc tgcgcggcca tccggagatt gagcctttag gcggcggcat atggatcggt
     2341 acggtcggat attatgccgc ttccctggag gtcgaagatc cgtttaacac cgtcatgaat
     2401 tttgacacgt cgggcggtac tcatcatata acgcagtcga taaagacggt cgggcgttat
     2461 cctgccaacg cgccgaacta caagggtgcg ataggctatg acggaaagaa tgtgaacggc
     2521 gtcgatattc ttgcgcctgc gttgaatctg tccgagacgc atgattttgc ggcgagcatg
     2581 ataaccatgg gctatatagc caacctcagc cgcaagaccg gcatgtacaa cagcgatctt
     2641 tttcgcgggt tcgagcctgg cgaagtgctt ttcgcaggcg ccagcggtac gaaaaccgaa
     2701 gacacgataa atcccatatg ggaagttacg ttcaatttca tggtgtcgcg caacagaaag
     2761 aacttccgcg tgggcgacat acaggtcagc ctcaaacgcg gatgggatta catgtgggtg
     2821 cgctacgaag atgctgtgga tgacgcggcc ggcgatgtca tcaaggcccc ggcagcagtc
     2881 tacatagaac aggtctacga atccacgagc tttgcttcgc tcggaataaa cagggcgctc
     2941 taagtttgcc ctattcttct gtttgaaatt gttcgagcag agtctttact tcaccccgcg
     3001 tcatcttgcg aagttttgcc agttcgattt ttgctgcatt ccgtattctt ttgagggatt
     3061 cgttttcgcg acgttcccat tgatatatgc tctgaccggt aacgccgaga aggacgccca
     3121 tatcggcctg cgatagttta agtttcgtac gcagtttgcg aatcatagtg ccggtcacgc
     3181 ggattttatc gacatttacc ttgagctgct tttcctcttc ctgctggcga agttcgctgt
     3241 tgcggactac ttcacgttca atctggcgtt tctggcggct cagatcattt acttccttgc
     3301 gaagtgtcgc gactgttttc ttcagcttga cgatatcttt cttcatgtcg ccggtaagat
     3361 tttttagctc tttgcgggcg actttggtaa tttcggcttt aaatgctttt aaaaattctg
     3421 ccattttgct tctcctgtag aaatttttta aaaagtattc tctcgaaaac gtcataagaa
     3481 tacatcgtga tgtttgaatc aactgtcatt ccatcatgcg gtttcccggt cttttgcagg
     3541 tctcataagg atagctcaaa aacttaaagg aatcaagaat tctttgaaaa ctctcaacgt
     3601 tttagcagat tttaaaaatc aaaataatgt tttttaagaa aatttttaaa aaattataaa
     3661 aaacaatggc acttaaaaaa gttttaaaag gcgacagctt cgatacgagg gccgagacgt
     3721 ggaacgcctt tatagaagcg gccaattacg tccgcaatca gcaggcactg accgccgccg
     3781 aatcaaaggg
//
LOCUS       DAONFI010000230         3790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29380_length_3790_cov_3.064793, whole genome shotgun sequence.
ACCESSION   DAONFI010000230 DAONFI010000000
VERSION     DAONFI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29380_length_3790_cov_3.064793"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLK08_07625"
     CDS             <1..338
                     /locus_tag="PLK08_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53405.1"
                     /translation="IGHIAGNKPWIMGGKCCCTPTRENYELHVKQGTIDKSVSYEDYL
                     NMYRSRGIVTDLNHHGCNNLCKYGPHVTLGGKCMATPQAGTENYRLVTYGPHKGPADS
                     VADDDKDKK"
     gene            429..1796
                     /locus_tag="PLK08_07630"
     CDS             429..1796
                     /locus_tag="PLK08_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53406.1"
                     /translation="MKSTTGFMAALLVFCVAVFMVDGSVFGQNSGLSMQQDAQRKLMA
                     HRAARADAMRQLAERIKGLRITSETTVNDFVTENDNIETSLRAWLMGMKEEGKYKDFE
                     DGTCSVEMSVTLKTVEAKLTSLYKAYYKGDKVKLQQIENLQTTNHLDKITVVGHGAMP
                     EELDDSAVVGASSAGVVPRTLNIPEIWKNHCTGQGRLMAARAARVDALRRLAERIKGL
                     RIDSETIVNDFVADSDEIQTRLNASLTGAKEVRVKYLKHELICEVTMQVKLKTILATL
                     RSAVDAEYKGDRVKLKRLDDYVENVRIDTIEETGSACPPAKYIRGYTQQQLTAVEQVS
                     GTNVPEWATRTLTVTGQAALDAKEYPNEAQARLMALRAAELVARRKLAEQVDGLRINA
                     DTTVKDFVGRNDTIRTRMMTYQVGAHVVEGSEKVRDGMAEVDVEMPLQPVWTTVSFFM
                     TRTAE"
     gene            1876..2025
                     /locus_tag="PLK08_07635"
     CDS             1876..2025
                     /locus_tag="PLK08_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53407.1"
                     /translation="MKKAIIILGLVVAMLVPAILAGCDDEVQVHQERTLAPRTVHQSE
                     VVTPD"
     gene            2142..3215
                     /locus_tag="PLK08_07640"
     CDS             2142..3215
                     /locus_tag="PLK08_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53408.1"
                     /translation="MTQESVVKVYGSGETQEEAIRDGLRKAVESVFVKVYSQSQTQDF
                     ALVRDTILTRAAGLVKEYKVLTAQTAADGVVTLELKVTVSDGRLDDLWGQVTTLLRQM
                     GRPKIMVCIVEKVDGKAQELSTLQTGLEQALLRNGFQLVDREQLKAIDKKDLEAAVAE
                     NSPTAVQSIAKRFGAQIFITGTASSDRAGKRMLYGRDFYEYQADSRVRVFRSDTAQLL
                     ASVVGSTPDASKGLQPSPNAAAAQALQRQAENVARKVTMDVLRFWTDVLTGRGEIKLS
                     VTGIADYREYVKIKNALQGIEGVSQVNATFHNGNADVSLESSLSAEMLAEKMATAMNA
                     LKITDVSQNTIKAEYSLKENGND"
     gene            3208..3564
                     /locus_tag="PLK08_07645"
     CDS             3208..3564
                     /locus_tag="PLK08_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53409.1"
                     /translation="MTETFLGKICVLTFMGCVAISIAGCNKFTMQRFDTMVYKGQTQP
                     EVEKVLGEPWMKTADMWYYRNNDNLDSAKIKFDGAGRVIDKQWDGRDVHPDAKPGAMN
                     DSPSAVKVETKEMVTP"
     gene            3582..>3790
                     /locus_tag="PLK08_07650"
     CDS             3582..>3790
                     /locus_tag="PLK08_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53410.1"
                     /translation="MAAAFEKYEKSFFAAVCLASLLFCVVAVADDVSGIWVDIKGQAA
                     GTDEHAREAAVKDAQINAIKAACGE"
BASE COUNT          985 a          877 c         1088 g          840 t
ORIGIN      
        1 gcatcggcca catcgctggc aataagccct ggattatggg cggcaaatgc tgttgcactc
       61 cgacgagaga aaattacgaa cttcacgtca agcagggcac tatagacaaa tccgtcagct
      121 acgaggatta cctgaacatg tatcgcagca ggggcatcgt cacagacctt aatcatcacg
      181 gctgcaacaa tctttgcaaa tatggcccgc atgttacgct tggcggtaaa tgcatggcga
      241 ctccgcaggc tgggacggaa aattatcggc tggtgactta tggcccgcac aaggggccgg
      301 ctgattccgt ggctgatgat gacaaggaca aaaagtagta gcaggacttt taaggtggtt
      361 ctcgaaagaa aatagttttg agaaccgttt taacaccata aatttttttt tcaagactgg
      421 aggcaattat gaaaagtacg acaggattca tggcggcgtt attggttttc tgtgttgcgg
      481 tgtttatggt tgacggaagt gttttcggcc agaacagcgg cctttcaatg cagcaggacg
      541 cccagcggaa gttgatggct catcgcgctg cgcgagccga tgccatgcgg cagttggctg
      601 aacgcatcaa aggcctgcga attacgtccg aaacgacggt caacgatttt gttacggaaa
      661 acgataatat tgaaacgtcg ttgcgggcat ggctgatggg tatgaaagag gaaggcaagt
      721 ataaggattt tgaggacggc acgtgttcag tggaaatgtc ggttactctc aaaaccgtcg
      781 aagccaaact gacgtcgctc tacaaagcct attacaaggg cgataaagtc aaactgcaac
      841 agatagaaaa tctccagacc acgaatcatc tggacaaaat aactgtcgtc ggccatggcg
      901 ccatgccgga ggagctggac gattctgccg ttgttggcgc ttcgtcagcc ggtgttgtac
      961 cacgcacgct taatatacct gagatatgga aaaatcactg taccggccaa ggccgtctaa
     1021 tggctgcccg tgctgcaagg gtcgatgccc ttcgccggct ggctgaacgc atcaagggcc
     1081 tgcggataga ttctgaaact atcgtaaacg attttgtcgc cgacagtgat gagatacaga
     1141 cacggctgaa tgcgtcgctt accggcgcga aggaagttcg cgtaaaatat ctcaagcatg
     1201 aactgatatg cgaagtgacc atgcaggtga agctcaaaac gattctggcg accctgcgaa
     1261 gcgctgttga cgccgaatac aagggcgacc gcgttaaact caaacggctg gacgattacg
     1321 tcgaaaacgt gcgaatagat actatcgagg agaccggcag tgcgtgtccg ccggcgaagt
     1381 atatccgcgg ctatacgcag cagcagttaa ctgccgtcga gcaggtcagc ggcacaaatg
     1441 tgcctgaatg ggctacgcgg acgcttactg tgaccggcca ggctgcactt gatgccaagg
     1501 aatatccgaa tgaagcgcag gccagattga tggctctgcg ggctgctgaa cttgtcgcac
     1561 gccgcaaact cgcggaacag gttgacggct tgcgtataaa cgccgataca accgtcaagg
     1621 atttcgtcgg caggaacgat accatccgca cacgcatgat gacgtaccag gtcggcgcgc
     1681 atgtggttga aggctcggaa aaagtcaggg acggcatggc ggaagtggac gtggaaatgc
     1741 cgcttcagcc ggtatggact acagtgtcgt tctttatgac aagaacggct gagtgattat
     1801 atttttcggc gtgaccagga tttgtgagtg tatgttgtac gaaaggcccc aaaattatca
     1861 ggagatattt ttattatgaa aaaggcaatc attattcttg gactggttgt ggctatgctt
     1921 gttccggcga tattagccgg ttgcgatgac gaagtacagg tgcatcagga acgtaccctt
     1981 gctccgcgca ctgtgcatca gtctgaagtt gtcacgccgg attgacgcga cttttgcgcg
     2041 tcgaaactgg attggatgtg attatgataa aaaagcatag ccttgttttt ttgctgtttg
     2101 tctgtgctgc gacagtgtcg gcatttgcac aacagcggta gatgacgcag gaaagcgtgg
     2161 taaaagtata cggcagcggc gagacgcagg aagaagcgat tcgtgatggt ttgcgaaaag
     2221 ccgtcgaatc ggtattcgtt aaggtgtact cgcagagcca gacgcaggat tttgcgttgg
     2281 ttcgcgatac gatactcacc cgcgcggctg gactggtgaa ggaatataaa gttctcactg
     2341 cgcagacggc ggcggacggc gttgtgacgt tggaactcaa agtgactgtc tcggatggcc
     2401 ggctggacga tttgtgggga caggtcacca cactgctcag gcagatggga cggccgaaga
     2461 taatggtctg catcgttgaa aaagtcgacg gcaaagcgca ggaactttcc acgctccaga
     2521 ccggccttga gcaggcattg ttgcggaacg gatttcagct tgtagacagg gaacagctca
     2581 aggctatcga caaaaaagac cttgaagccg ctgtggctga aaattcgccg acagccgtgc
     2641 aatctatcgc caagagattc ggcgcccaga tattcataac cggcacagcc agttcagacc
     2701 gtgccggcaa acgcatgctc tacggcaggg atttttatga atatcaggct gattctcgcg
     2761 tgcgtgtttt ccgcagcgac accgcacaac tgctggccag tgtggtcggc tctacgccgg
     2821 atgcgagcaa aggccttcag ccgtcgccta acgccgcagc cgcgcaagcc ttgcaacgcc
     2881 aggctgaaaa cgtcgcacga aaggttacca tggatgtgct gcggttctgg acggacgtat
     2941 tgaccggccg cggcgaaata aaactctctg tcacaggaat tgccgattac cgcgaatatg
     3001 tgaaaataaa aaatgctttg cagggtatcg aaggcgtcag ccaggttaac gcgacgttcc
     3061 ataacggcaa tgccgacgta tctctggagt cgtcgctttc ggcggaaatg ctggcggaga
     3121 agatggccac tgcgatgaac gcactgaaaa ttaccgacgt ttcgcaaaat acaatcaaag
     3181 ccgaatactc tctgaaggag aatggaaatg actgaaactt ttttgggtaa aatttgcgtt
     3241 ttgacattca tgggctgtgt tgcgatttcg atagccggct gtaataagtt cacgatgcag
     3301 cggtttgaca ccatggtcta taaaggccag actcaacctg aagtcgaaaa agttctgggt
     3361 gagccgtgga tgaagacagc cgacatgtgg tattatcgca ataacgacaa cctcgactcc
     3421 gcaaaaatca agtttgacgg cgccggccgc gttatagaca aacagtggga cggccgtgat
     3481 gttcaccctg acgccaagcc aggcgcaatg aacgattcgc cgtctgcggt gaaagttgaa
     3541 accaaagaaa tggtaacgcc atgacgcgtt ggagtttttg aatggctgct gcgtttgaaa
     3601 aatatgaaaa gtcgtttttt gccgccgtgt gtcttgcaag tctgctgttt tgcgtcgttg
     3661 cggttgcgga tgatgtgagc ggcatatggg tggacatcaa aggccaggct gccggcactg
     3721 acgagcatgc ccgcgaggct gctgtgaaag acgcccagat aaatgccata aaagccgcct
     3781 gcggagaaaa
//
LOCUS       DAONFI010000231         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29716_length_3759_cov_4.860421, whole genome shotgun sequence.
ACCESSION   DAONFI010000231 DAONFI010000000
VERSION     DAONFI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29716_length_3759_cov_4.860421"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..673
                     /locus_tag="PLK08_07655"
     CDS             161..673
                     /locus_tag="PLK08_07655"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HPS53411.1"
                     /translation="MRDKHLLHGFTHENPVPGTCEWFTPPWLFEAMDIEFDLDPASPG
                     KAAVPWIPAREHFTLNENGLVQPWHGNVWLNPPYGRQTPVWLKKILSHGRGIALVFAR
                     TDTRWFHETAVNFDAICFLRKRINFIRHDGSEGSSPGCGSMLLVCGRDNVKAVRKMKS
                     GITVEPQKGF"
     gene            677..1246
                     /locus_tag="PLK08_07660"
     CDS             677..1246
                     /locus_tag="PLK08_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53412.1"
                     /translation="MLNENYRISSFNSRWATLSWTCSEASLFSWIIINGANLYGPVIS
                     ESTERTVRVPFKAGAIVTLEVHDLCEADVPEAIYPPVNTSPTLRWNFLDGAVRYKIYH
                     RAGGTADKKVYDSVAYVNQIVSPVELEGSGELDNCQYHFLRVEAVDCYGNESTRQSWR
                     FFSKDLPASDVSLKISQGSSSGIFDITLE"
     gene            1251..2519
                     /locus_tag="PLK08_07665"
     CDS             1251..2519
                     /locus_tag="PLK08_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53413.1"
                     /translation="MATPTADKLRTYFPSRYIAADGNAMTEYVVDSATLRNITDAALN
                     EADDYWNGALGWFVGDTLTADLRGCFFHVKDFDAASDTLTLSKDLPAQPAAGDKFYMV
                     IGGNRRSGCETFGLMVASNQPELQPYHCSSITGVTIKKVSGQLGEGDVTLKYDPAAGE
                     IYLQKADEAYGVGIDVTSPLTDAIVFSADGQSYLQFDVVSTSLPAIEKTEVLTLAINQ
                     GVFTPDFEGYETSAVGGKVRYRLEILRNEDSADTMIDLIVYLKKTSGVATAITSETDL
                     TPDESEVEIEDSTDWPTNSFWVYNKTRDDCRYIKYRSGKTLYTTDAGAGLRDFAAVNW
                     VTGDEVEVMGDIDIALEVPDVSGVFSNPADESTAPGGLNFATHCGINNAIYYGDLDPG
                     EIIGIWRRETVVENHRARNSIRTDMITVWS"
     gene            complement(2532..2828)
                     /locus_tag="PLK08_07670"
     CDS             complement(2532..2828)
                     /locus_tag="PLK08_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006170555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HPS53414.1"
                     /translation="MKTKTTKYDVAEHLRTPEEMAAYLEACMEESDGDAAFVAKALGD
                     IARAKGMTQIARDSGLSRESLYKALSGERTPTFDTILKVVRALGIELHATPIQA"
     gene            complement(2839..3129)
                     /locus_tag="PLK08_07675"
     CDS             complement(2839..3129)
                     /locus_tag="PLK08_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPS53415.1"
                     /translation="MMEIRKTETFAEWLDGLRDMRARARVQVRIERLAMGLSGDAEPV
                     GEGVSELRINYGPGYRVYYKKCGKTLIILLVGGDKRTQAKDIKTALRLARNL"
     gene            3201..>3759
                     /locus_tag="PLK08_07680"
     CDS             3201..>3759
                     /locus_tag="PLK08_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPS53416.1"
                     /translation="MSTKLLLQFNNSFHDGSGNERGITTSGYFAFTDSGPFGYGLEPK
                     IGKVDVADSTDWQIGSGDFTVDFWAMVQSSAGRDQYPFELGDNRELSLRFLDYYGRIY
                     IYMNGSYVYTADNVFLNDTWFHVALIRSSGVVRLFINGSQVYEYSNTVDILPVGGFAV
                     GNRISGSYWMGGIAEFRVCDTAEMSW"
BASE COUNT          921 a          936 c          975 g          927 t
ORIGIN      
        1 acggcggtac gaaaactcca gacccgctca atccgcttca gcccatcgca tggcgtgagc
       61 atcgcgacag cacacagttc tggttgcaat ccacgtgcaa ctggtacgag tccctgcgtc
      121 aggaatattc ctgaacatga cagcaataaa ggtgaatgca atgagagata aacatctgct
      181 ccacggtttt acccatgaaa atccggttcc cggtacgtgc gaatggttca cgccgccgtg
      241 gctttttgag gctatggata tcgaattcga cctcgatccg gcgagtcccg gcaaagctgc
      301 cgtgccgtgg ataccggcaa gagagcattt tacgcttaat gaaaacggtc tagtccagcc
      361 ctggcacggc aacgtctggc tgaacccgcc gtatggtcgc cagaccccgg tatggctcaa
      421 aaaaattctt tcacacggca gaggaatcgc actcgttttc gcccgaacgg atacgaggtg
      481 gttccatgag acggcggtta atttcgacgc gatatgtttt ctgcggaagc ggatcaattt
      541 catccgtcat gacggaagcg agggttccag tccgggctgc ggttcgatgc ttctcgtctg
      601 cggcagggat aacgtgaaag ctgtcaggaa aatgaaatct ggaattacgg tagaacccca
      661 aaaaggtttt tgaaaaatgc ttaacgaaaa ttacaggata tcgtcgttta actcaaggtg
      721 ggcgactctc agctggacgt gcagtgaggc gagtctgttc agctggataa tcattaacgg
      781 cgcgaactta tacggaccgg taataagcga gtctacggaa agaaccgtcc gcgttccgtt
      841 caaggccggg gccatagtta ctctggaagt ccacgacctt tgcgaagccg acgttcccga
      901 agccatctat cctccagtca atacctctcc gacgttacgt tggaattttt tagacggtgc
      961 cgtacgctac aagatatacc atcgcgcagg cggcaccgct gataaaaagg tgtacgactc
     1021 ggtcgcatat gtaaaccaga ttgtttctcc ggtcgaactt gaagggagcg gcgaactgga
     1081 taactgtcag tatcatttcc tgcgcgtcga ggccgtagat tgctacggca acgaatcgac
     1141 gcggcagtcc tggagatttt tcagcaaaga tttaccggcg agcgatgttt ccctgaaaat
     1201 atcgcagggt tcgtcatcag gaatttttga catcactttg gagtaaaacc atggctacac
     1261 ctaccgccga taaattgagg acgtatttcc cgagccgata tattgcagcc gacggcaacg
     1321 cgatgactga atatgtcgtc gattcagcta cgttgcggaa tataaccgat gctgcattga
     1381 atgaggctga cgattactgg aacggcgcgc tgggctggtt tgtaggcgat acgctgaccg
     1441 ccgacctgcg aggctgcttt tttcacgtaa aggattttga cgcggcaagc gatacgctta
     1501 cgctcagcaa ggatttgcca gcccagcccg cagctggcga taaattctac atggtaatcg
     1561 gcggcaatcg tcgcagtggc tgcgagacgt tcggactcat ggtcgcgtct aatcaacctg
     1621 aactccaacc ttatcactgt agcagtatta ccggcgtaac tataaaaaag gtgtcaggac
     1681 aacttggcga aggcgacgtt acgttaaaat atgatccggc tgcaggtgaa atatatttgc
     1741 aaaaagctga tgaagcatac ggtgtcggta ttgacgtaac ttcgccgctg accgacgcga
     1801 tagttttttc agccgatggg caaagctatt tgcagttcga cgttgtctca accagtctgc
     1861 ctgctattga aaaaactgaa gtattgacac tcgcgataaa tcagggcgta ttcactcccg
     1921 attttgaggg ttacgaaact tccgccgtag gcggtaaggt tcgatatcgc ctggaaattc
     1981 tgcgcaatga agattcagcc gatacaatga tagacctcat cgtctatctt aaaaaaacat
     2041 caggcgtcgc tactgcgata acttccgaga ccgacctcac gcccgacgag agtgaggtcg
     2101 agattgagga ttccactgat tggccgacca attcattctg ggtctataac aagacacggg
     2161 acgattgcag atatatcaag taccgtagcg gaaaaacgct ttataccact gacgccgggg
     2221 ctggacttcg agattttgca gccgtcaact gggtaacggg cgatgaagtg gaagttatgg
     2281 gcgatatcga tattgccttg gaagtgcccg acgttagcgg ggttttcagc aatcctgccg
     2341 acgaatccac cgctccgggc ggacttaact tcgctactca ctgcggaatc aataatgcaa
     2401 tctattacgg cgacctcgac cccggcgaaa tcataggcat atggcggcgt gaaaccgtgg
     2461 tcgagaacca ccgcgccaga aactcaatac gaacagatat gattacagtc tggagctgat
     2521 tttcttccgg tctatgcctg aatgggagtg gcgtgcagtt ctataccgag tgcgcgtacg
     2581 accttgagaa tcgtatcgaa agtcggagtg cgctcgccgg aaagggcttt gtaaaggctt
     2641 tccctggaaa gtcctgaatc tctggcaatc tgggtcattc cctttgcccg tgcgatatcg
     2701 ccgagagctt tggcgacgaa agcggcgtcg ccgtctgatt cttccatgca ggcttcgagg
     2761 taggcagcca tttcttcagg agtgcggaga tgttcagcca cgtcgtattt ggtagttttt
     2821 gttttcattg ttcttctcct acaaattgcg tgcgagtcgt aatgccgtct tgatatcctt
     2881 ggcttgcgtc cgtttatcgc cgccaaccag caggattatc aatgtttttc cacatttttt
     2941 gtaatataca cggtatcccg gcccataatt aatccgcagt tccgagactc cttcgccgac
     3001 aggttcggca tcgccggata gacccatggc gagtcgctcg attctgacct gaacgcgggc
     3061 acgggcacgc atgtcccgaa gaccgtcaag ccactcggca aaggtttcag ttttacggat
     3121 ttccatcatc ttttatattg tagcctactg gctacaataa ttcaacagca aaattattca
     3181 aactttggat ttattttatt atgagcacaa aactcttact gcaatttaac aattcttttc
     3241 acgacggcag cggcaatgag cgtggcataa ctacgtctgg atattttgcg tttacggatt
     3301 ccgggccgtt cggttacggc ctggaaccca aaatcggaaa ggtcgatgtt gcggactcga
     3361 ccgactggca gataggcagc ggcgatttta ccgtggattt ctgggcgatg gtacaatcgt
     3421 ctgcaggccg cgaccagtac cccttcgagc taggcgataa ccgcgaactt tctctgcgat
     3481 ttctggacta ttacggcaga atctacatct acatgaacgg ctcatacgtt tatacggctg
     3541 ataacgtctt tttaaacgat acgtggttcc acgtcgcact tatccgctca agtggagtag
     3601 tccgcctgtt catcaacggt tcgcaggttt acgagtacag caacaccgtc gatattctgc
     3661 ctgtcggcgg atttgccgtc ggtaaccgca taagcggaag ctactggatg ggcggcatag
     3721 ctgaattccg ggtgtgcgat accgccgaaa tgtcctggt
//
LOCUS       DAONFI010000232         3749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29834_length_3749_cov_3.890633, whole genome shotgun sequence.
ACCESSION   DAONFI010000232 DAONFI010000000
VERSION     DAONFI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29834_length_3749_cov_3.890633"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLK08_07685"
     CDS             complement(<1..280)
                     /locus_tag="PLK08_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53417.1"
                     /translation="MSRFVNQNLMQITEEILASYDMDPKTQRIGERFLPSRTIIVEIL
                     EEIRRLIFPGFFGHKMLTPENVRYHVGNLIIQLSDRMTEQINYCICSSK"
     gene            464..1318
                     /locus_tag="PLK08_07690"
     CDS             464..1318
                     /locus_tag="PLK08_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPS53418.1"
                     /translation="MRTYHECRPCFTRQAVEVGRMMAADERLARQIVDETQKITDAMD
                     MTQPPPLASMNMYRMIRRLTGVNDPYHDYKRKTNRQLMGMIERLRREIRQSADPLLAA
                     VLGAVSGNMIDCGINGQVSDEQIRAAFHDINETALIGEIGTFRSEVERAEKILYLADN
                     SGEIVLDRLLIELLPAEKLTFVVRGGPIINDVLMEDAAEVGLTDMVRVMDNGSDAPGT
                     LLSQCSPEFQRAFDEADLIISKGQGNFETLNDMKGRNIIFLLKIKCEVISRHTSMPLG
                     TPLLYHVR"
     gene            1497..3188
                     /locus_tag="PLK08_07695"
     CDS             1497..3188
                     /locus_tag="PLK08_07695"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HPS53419.1"
                     /translation="MEGSAVKNNKLQTVLTISFVAFMMFLAAGCGGSPSFATSCCYDP
                     NPQQAASKAVAEAVEKLKGKPVKGLIFAVYYEKASFTPKEPGDYMPDVERECIAAETI
                     ARIAKELAVKQELGEKPTEKKQYPVVPPTVTETSPASKPAEEPASQPAVAATNPADKA
                     AAEKAAKEAAKAAAKLAAKKEAAEKEAARRAAAEKALAERIDKIQPLPNIGLRARAFV
                     DGHEMQPNSVAVLAVAGDTECTAVKIPILADRGKTGQLLAYSVKKMENIKLVFVFSEM
                     NLNFAPEAQGKTQAFIDKALAGLPKNVVLFGGNSMNNPADKQNILAGGQYFNGHQASG
                     SIVAMAIAGNMKVFTDQANEFTAVPRTVLVTESRGPWVITLDDRPAAEVYRRIADMPA
                     NEKFTSDWQHPIGIPVSRNKIYLQMVTNWIDKDGKDKDGKKLPLPAGSLRFRSDIPYG
                     TQICVLTGGNDSQKILDSLRDCTKSAIEQAHDSGYKPSLILVCECCARNMRLEAMRDA
                     SSKNSANVLTRVAGKNSPAIFGFYAFGHFAPIGGTFLKSSTHFQQHVVTSIVVAE"
     gene            3212..3721
                     /locus_tag="PLK08_07700"
     CDS             3212..3721
                     /locus_tag="PLK08_07700"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPS53420.1"
                     /translation="MITLLVAMDEDRVIGHGSELPWHLPEDLKLFKRRTMGHTIIMGR
                     KTWQSLPVSHLPGRVNMVITRQPEKYAKTVKDFDAVEGPHFITTFELAVSTAHREFPE
                     YAQEIFLIGGEQLYDLALRRNLCDRMIITHVYGKHMGDRYFPQFGPIWKIGKTEKHND
                     FDVTEYTRV"
BASE COUNT          984 a          998 c          975 g          792 t
ORIGIN      
        1 ccttcgacga gcatatgcaa taatttatct gctctgtcat cctgtccgag agctgtatta
       61 tcagattgcc tacgtgatat cgcacattct ccggcgtcag catcttgtgg ccgaaaaacc
      121 ctggaaatat cagccggcgg atttcctcca gtatttccac aattatggta cggctgggca
      181 gaaaacgctc gccgattcgc tgcgtcttgg gatccatgtc gtaactggcg agaatctctt
      241 ccgttatctg cataagattc tgattcacaa atctgctcat aagtctgtcc tcgaaaaatg
      301 ttttgcatca ttttagcttc ggcaagccgc catttccaag ctttttaaca agtgctaccg
      361 taattccgcc atttttttga gataggttca taaaaaacag gttttgagtt gagagatttt
      421 cagcatgtga tatcatgtcc gattttgcgg agtttttagc gagatgcgaa cctatcacga
      481 atgcaggcca tgtttcacgc gacaggctgt cgaagttggc agaatgatgg cggctgacga
      541 aaggctggcc cgtcaaatag tcgacgaaac tcagaaaatc accgacgcca tggatatgac
      601 ccagccgccg ccgctggcgt ccatgaacat gtatcgcatg atacgccgcc ttaccggcgt
      661 caacgaccct tatcatgatt acaagcgcaa gacgaatcgc cagttgatgg gcatgataga
      721 gcggttgcgg cgcgagattc gtcagtcagc cgacccgttg ctggcggcgg ttctcggcgc
      781 ggtcagcggc aacatgatag attgcggcat taacggccag gtctcggacg agcagatacg
      841 cgctgcgttt cacgacatca acgaaacggc acttatcggt gaaatcggga cttttcgcag
      901 tgaagtcgaa cgggctgaaa aaatactgta tcttgcggac aattccggcg aaatcgtgct
      961 tgaccgcctg cttatagagc ttctgccggc ggagaagcta acgttcgtcg tccgcggcgg
     1021 gccaatcatc aacgacgtac ttatggaaga cgccgcggaa gtgggcctga ccgacatggt
     1081 tcgcgtcatg gacaacggca gtgatgcgcc tggaacgctt ctgtcccaat gcagccccga
     1141 gtttcagcgg gcatttgacg aggctgacct gataatatcc aaaggccagg gcaattttga
     1201 aacgctcaac gacatgaaag gcaggaatat aatctttctg ctgaaaatca agtgcgaggt
     1261 aatatccagg catacgtcca tgcctctcgg cacgccgctg ctgtatcacg ttcgatgaaa
     1321 acgcggcaca atagccgatt acatgcgcct ccacgtcgca aacgggtaat tttttgcatt
     1381 ttgcctttat ttctggtatc ctgcgtcgaa ttgtactggc tgcgcgtatt tctttgaaaa
     1441 tgtcatacga cgcggttaaa atatgccccc tggtgtcatc ggattggtac agaagaatgg
     1501 aagggtcggc tgtgaaaaac aataaattac agacagttct gacaatatcg ttcgtagcat
     1561 tcatgatgtt tctggcagcc ggttgcggcg gcagcccgag ctttgcgacc tcatgctgtt
     1621 atgacccaaa tccgcagcag gcagccagca aggcggtagc agaagccgtc gaaaaactca
     1681 agggcaagcc ggtcaaaggc ttgatattcg ccgtctatta cgaaaaagcg tcctttactc
     1741 ccaaagaacc tggcgattat atgcctgacg tcgaacgcga gtgcatcgcc gcggaaacca
     1801 tcgcacgaat cgccaaggaa ctcgccgtga agcaggaact cggcgaaaag ccgaccgaga
     1861 agaaacaata tcccgtcgta ccgccgacag taacggaaac ttcgccggcc agcaaaccgg
     1921 cagaagagcc agccagccag ccagccgtag ccgctacaaa tccagccgac aaagccgccg
     1981 ctgaaaaggc ggcaaaagaa gctgcgaaag ctgccgcaaa attagccgcc aaaaaagagg
     2041 ctgcggaaaa agaagccgcc cgacgtgcgg cagctgaaaa ggcactcgcc gaacgaatcg
     2101 ataaaattca gccgctgccc aatatcggcc tgcgtgccag ggcattcgtg gacggccatg
     2161 aaatgcagcc gaacagcgta gcggtgctgg ctgtggcagg cgacacggaa tgtactgccg
     2221 taaagatacc gatactggct gaccgcggca aaaccggcca actgctggca tacagcgtaa
     2281 aaaaaatgga aaacatcaag ctggtgttcg tcttttctga gatgaatctc aacttcgctc
     2341 ctgaagccca gggcaaaacc caggcgttca tcgacaaggc tctcgccggc ctgccgaaaa
     2401 atgtcgtcct cttcggcggc aatagcatga acaatccggc cgacaagcag aacatcctgg
     2461 ccggcggcca atatttcaac ggccaccagg ctagcggcag catagtagcc atggccatcg
     2521 ccggcaacat gaaagtattc accgaccagg ctaacgaatt tactgccgtc ccacgcaccg
     2581 tcctggtgac ggaatccagg ggcccctggg ttattacgct cgatgaccgg ccggctgcgg
     2641 aagtctatcg tcgcatcgcc gatatgccgg cgaatgaaaa attcacaagc gactggcagc
     2701 atcctatcgg cataccggtc tcacgtaaca agatatatct gcagatggtg acgaactgga
     2761 ttgacaagga cggcaaggac aaggacggca aaaaactgcc gctgccggct ggctcgctgc
     2821 gattcagaag cgacataccg tacggtacgc agatttgcgt gctgaccggc ggcaacgatt
     2881 cgcaaaaaat tctggattcg ttgcgggact gcaccaaaag tgccatcgaa caggctcatg
     2941 acagcggtta taagccgtca ctgattctgg tctgcgagtg ttgcgcgaga aacatgcggc
     3001 ttgaagccat gcgggatgct tcgtcaaaga attctgccaa cgttctcaca cgcgtagccg
     3061 gcaaaaattc gccggcgata ttcggcttct atgcgttcgg acatttcgct ccaatcggcg
     3121 gaacttttct gaaaagctcc acccactttc agcagcatgt cgtcaccagc atagtggtgg
     3181 ctgagtaaaa ttattccgat ttgaggttgg catgataaca cttctggttg caatggatga
     3241 ggacagggtt ataggccatg gcagcgaact gccgtggcat ctgccggaag acctgaaact
     3301 tttcaaacgt cgcacgatgg gccataccat catcatgggc cgcaaaacgt ggcaatccct
     3361 gccggtcagc cacctgcctg gccgcgtcaa catggtcata acccgtcagc cggaaaaata
     3421 cgccaaaaca gtcaaagact tcgacgccgt cgaagggccg catttcataa ccacgtttga
     3481 actcgccgtc tctacggcac atcgcgaatt tccggaatac gcccaagaga ttttcctcat
     3541 cggcggtgaa cagctgtacg acctggccct gcgccgcaat ctgtgcgaca ggatgataat
     3601 tacgcacgta tacggcaaac atatgggcga cagatatttt ccgcaattcg ggccgatatg
     3661 gaaaatcgga aaaaccgaaa agcacaacga tttcgacgta accgaatata caagggttta
     3721 agaacatcat gcgattgacc ggacttttg
//
LOCUS       DAONFI010000233         3748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_29842_length_3748_cov_4.015164, whole genome shotgun sequence.
ACCESSION   DAONFI010000233 DAONFI010000000
VERSION     DAONFI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29842_length_3748_cov_4.015164"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLK08_07705"
     CDS             <1..370
                     /locus_tag="PLK08_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPS53421.1"
                     /translation="IIRNGGEFWEIHINGGRIPTGKYAVEWAKEVEDRGAGEIMLTVM
                     DADGTQNGYDIEITRAVSDAVRIPVIASGGAGSPEHIRQALDEGHADAALAASIFHFG
                     KYSIRETKEYLASRGVPVRL"
     gene            385..1581
                     /locus_tag="PLK08_07710"
     CDS             385..1581
                     /locus_tag="PLK08_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPS53422.1"
                     /translation="MAESTAPRPVPIPISVAVKQPEPLPTDGIEVPADVKIPAGLYKF
                     FRAMTLVNASDLHLKVGTVPHVRFESGLRKCGHPATTEAEMTEIVKSLLNERQYAVFQ
                     ETGGFDVAYQIVGGDRYRINIYRQRGLISLAVRRVTCNIPDFKQLHLPESLHDVCTYS
                     QGLVLVVGPTGSGKSTTIAAILEHINQTRPCHIVTIEDPIEFVYQDKLSLVNQREVGI
                     DVADFHQGIRSMLREDPDVVLVGEMRDQETFSAAMQAAETGHLVFGTLHAGSAPQAIS
                     RIVDLFSPESRPLLLQSMAYNLKAVICQRLLPCVRKDIDRVPAVEILLNNPVVREKLE
                     KGKENELGDVMKSNAVDGMLTFTQSLYDLIQNDMIDPRDAYAVAPSAVELKMMLKGIN
                     QSGKSF"
     gene            1695..2252
                     /locus_tag="PLK08_07715"
     CDS             1695..2252
                     /locus_tag="PLK08_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPS53423.1"
                     /translation="MIIKPYILSQINMGDFSIAGYSVAGEESVIMSPELDVCFDIGKC
                     PREALSINNVFLTHGHADHSAGLLYYFSQRDFQAIAGGRIFLPHRMLDAVEALISAWG
                     KVEGHLPPYELVGMGDGEEFEIRRDLFVKAFATRHVPGSLGFSIVEERRKLKEEFLGL
                     DSPQIVELKKKGVEISNTIELPLVA"
     gene            2253..2585
                     /locus_tag="PLK08_07720"
     CDS             2253..2585
                     /locus_tag="PLK08_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53424.1"
                     /translation="MGDTAKADYSGNPYIRDARVLLMECTFFDDEHVDRAKAGRHIHV
                     DELAEVLAGMNNEKIILTHVTRRTNLGYARRMLKKSLPAEILAKTTFLMSREHIPEKL
                     EPENESEN"
     gene            2749..3483
                     /gene="budA"
                     /locus_tag="PLK08_07725"
     CDS             2749..3483
                     /gene="budA"
                     /locus_tag="PLK08_07725"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HPS53425.1"
                     /translation="MFSGCGQQQKYGDNLYQVSTLQALMDGMYDGQVSIGELKRQGCE
                     GIGTVDALDGELVVLDGGAWHIRADGKAYPLKNSDTTPFADVCSAHSVTSLNIPSGWS
                     YEKFRELLDSKAGNMNFPLAVRVDGTFSYVKTRSVPAQKKPYPPLTEVVKHQPVFELH
                     NVRGTLVGFRMPEYFKGVNMPGYHLHFITADRSEGGHVLDFTASSAAAQVGYCTNVRI
                     TLPATEDFAKADLGRDMSAEIHKAEK"
BASE COUNT          950 a          945 c         1022 g          831 t
ORIGIN      
        1 aataatccgc aacggcgggg aattttggga gatacacatc aacggcggcc gaatcccgac
       61 cggcaaatac gccgtcgaat gggcaaaaga agtcgaagac cgcggcgcgg gcgaaatcat
      121 gcttaccgtc atggatgccg acggcactca aaacgggtac gacatcgaaa tcacgcgagc
      181 cgtttccgat gcggttcgca taccggtaat tgcatccggc ggagcgggca gtccggagca
      241 cattcgccag gcgctggacg aaggccatgc cgatgcggcg ctggcggcgt cgattttcca
      301 tttcgggaaa tattcaatac gtgaaaccaa agaatatctg gcctcccgcg gcgtaccggt
      361 cagactgtaa tatggaggaa gaaaatggct gagtcaaccg ccccaaggcc agtgccgatt
      421 ccgatatccg tagcagtgaa gcagcccgaa cctctgccga cagacggtat tgaagtgccg
      481 gctgacgtga agataccggc agggctctat aaatttttca gggctatgac gcttgttaac
      541 gcatccgacc ttcatctgaa ggttggcact gtgccgcatg tgcgttttga aagcggcttg
      601 cggaagtgcg gacacccggc caccaccgaa gccgaaatga cggaaatcgt caaaagtctg
      661 ctcaacgaac ggcagtatgc cgtttttcag gagacaggcg gattcgacgt cgcctaccag
      721 atagtcggcg gcgaccgtta tcgaatcaac atatatcgac aacggggact gatatcgctg
      781 gctgtccgcc gcgttacgtg caatataccg gacttcaaac aactgcatct gcccgaatcg
      841 ctgcatgacg tctgtacgta ttcccaaggc ctggtactcg tagtcgggcc gacgggcagc
      901 ggcaaaagca cgactattgc ggcgatactc gaacatataa accaaacgcg gccatgtcat
      961 atcgtcacaa tagaagaccc catagaattc gtatatcagg acaagctctc gctggtcaat
     1021 cagcgtgagg tcggcattga cgtggcggat tttcaccagg gtatacgttc catgcttcgt
     1081 gaagacccgg acgtggtgct tgtgggcgaa atgcgcgacc aggagacgtt cagtgcggcg
     1141 atgcaggctg ccgaaaccgg ccatcttgta ttcggcactc tgcacgccgg cagtgcgccg
     1201 caggcgatat cgcgaattgt cgacctgttt tccccggaat cgcggccgct gctgcttcaa
     1261 tcgatggcgt acaatctgaa ggctgttatc tgtcagcggc tgctgccgtg cgtcagaaaa
     1321 gatatcgacc gtgtgccggc ggtggaaatt ttgctcaaca accccgtcgt gcgcgaaaaa
     1381 ctcgaaaaag gcaaggaaaa cgaactcggc gatgtgatga agtcaaacgc cgtcgacggc
     1441 atgctgactt tcacgcaaag cctttacgac ctgatacaga acgacatgat agaccccaga
     1501 gacgcctacg ccgtcgcgcc tagcgctgtg gaactaaaga tgatgctgaa aggcataaat
     1561 cagagcggca aaagtttcta aagtttctga tgcgacgtag cctgcggaaa acatccgccg
     1621 ccatgcggac tggcgaaagc cggataaata tatgctctag actgcagaat gcgttttatt
     1681 ttttggattt ttgaatgatt ataaagcctt acatactttc gcaaatcaat atgggagatt
     1741 tttccatcgc cggttatagc gtcgccggcg aggagtccgt aataatgtcg ccggaactgg
     1801 acgtttgttt cgatatcggc aaatgtccgc gtgaggcatt gtcgataaac aacgttttcc
     1861 tgacgcacgg ccatgccgac cacagtgcgg gattgctgta ttatttttca cagcgggatt
     1921 ttcaggcgat tgccggcggc aggatttttc tgccgcacag gatgctggat gccgtggagg
     1981 cgttgatttc cgcctggggc aaggtggaag gccatctgcc gccgtatgaa ctcgtaggca
     2041 tgggcgacgg ggaggaattc gagattcgtc gcgacctttt tgtaaaagcc tttgccaccc
     2101 gacatgtccc gggctcgctt ggtttttcca tcgtcgaaga gcgtcgcaag ctcaaggaag
     2161 aatttttggg cctggatagc ccacagatag tcgaactgaa aaaaaaaggc gtcgaaattt
     2221 ccaataccat agaattgccg ctcgtggcgt aaatgggcga taccgcgaag gctgactatt
     2281 ccggcaatcc gtatatccgg gacgcccgcg tcctgctgat ggaatgcacg ttttttgacg
     2341 acgagcatgt cgacagggcc aaagccggcc gccatatcca tgtggatgaa ctcgccgaag
     2401 tgttagccgg catgaataat gaaaaaataa tcctgactca cgttactcgg cgaacgaatc
     2461 tgggctacgc aaggcgaatg ctcaaaaaat ccctgccggc ggaaattctg gcgaagacga
     2521 cgttcctcat gagccgcgag cacatacccg aaaaactgga acctgaaaat gaatcggaaa
     2581 attgaaataa cattatccat attcggttac catgcccaca caaagtgtgg gcgtgcttac
     2641 cgtagtttag agcgtacatg cccacgaggc gtgggcatgg taaccgggtt ttctgctttg
     2701 ctgttgaaac gagtatttgt gctatttatc ttgtgtgtcg gtctcacgat gttttccggc
     2761 tgcggtcagc agcaaaaata cggagataac ctttaccagg tttccacgct gcaggcgctt
     2821 atggacggca tgtacgacgg tcaggtttcc atcggcgaac ttaagaggca gggctgcgag
     2881 ggtatcggaa cggtcgatgc gcttgacggt gaactggtgg tactcgacgg aggggcgtgg
     2941 catattcgtg ccgacggaaa agcctaccct ctcaaaaatt ccgataccac cccgtttgcc
     3001 gacgtgtgca gcgctcattc cgtaacatcc ctgaacattc catctggctg gagttatgaa
     3061 aaattccgtg agctgttgga ttcgaaggca ggtaacatga atttcccgct cgccgtccgc
     3121 gtggacggta cgttcagcta cgtaaaaacc cgtagcgtac ccgcccagaa aaaaccgtat
     3181 ccgcctctga cggaagtggt taagcatcag cctgtattcg agcttcacaa cgtccgcggt
     3241 acgctggtcg gttttcgcat gccggaatat ttcaagggcg taaacatgcc cggctatcac
     3301 ctgcacttca taactgcaga ccgcagcgag ggcggccacg tgcttgattt tacagcctct
     3361 tctgccgcgg cgcaggttgg ttactgtacg aatgtgcgaa taaccctgcc ggctaccgag
     3421 gacttcgcca aagccgattt aggccgtgat atgtctgcgg aaattcataa agctgaaaaa
     3481 taatcagccc gcccgatggc tgaaaatcca atttccgggg tcaattattc aaaaaaatat
     3541 ataaaaaatg cttgaaaccg gcgggagaat ccatatcata ccggcggtta attttttggt
     3601 atagtgggca gagtttgtcc catattttta taaaaagatt ccatgaaagg ataagtgagt
     3661 cttatatgct gaaagtcaaa gcacgcggca gcgaatccat cgatcagatg ctcaaacgct
     3721 tcaaaaagat gtgtgaaaag gaaggcct
//
LOCUS       DAONFI010000234         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_30024_length_3733_cov_3.430397, whole genome shotgun sequence.
ACCESSION   DAONFI010000234 DAONFI010000000
VERSION     DAONFI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30024_length_3733_cov_3.430397"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLK08_07730"
     CDS             <1..258
                     /locus_tag="PLK08_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53426.1"
                     /translation="RTKFYLAFEKPHRPYLFGEGIAMAVTLGWLSGVVLLTEIHVNSI
                     IQLPVRDFWTLIPICIALALLASLLIGAIISLLWTAISLNK"
     gene            877..3339
                     /locus_tag="PLK08_07735"
     CDS             877..3339
                     /locus_tag="PLK08_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53427.1"
                     /translation="MRKALPRDIVRMLTTWSGMAMMVALLPVGMIFWHPGYRAWGIVA
                     VGVIIAMLIWMFRGLHERTNAVAGNPIHWVWLSMAVYLSVIQFFGQYCPKIETSSGIR
                     GFLNISVILQLLTLSLGCLLFQEFMSKGQIARWLREISAVFIIVLSLSLDSLFSPETP
                     QTAMWLTGCTGVMMAAANAFSAGSSVVVGVSGINRRHQIAIWGQLIIATAGFLCLLSM
                     CTAKSFCLFFVITATVAGIAFICRKRLAASVKFYMTLGILWWLIIIAGCLGFYMCGDG
                     VFTFWGHGEKGARYFWGIDDGITVLFNLVGFVGVGGMFAGLVAVSMAVIVKNVSRSDG
                     NFWKAVLWLTACWLATAAWMSGKTFFSVMPLPIMMMSWGLLFTFSDVRVRWHRAWRFA
                     LPMALGLFWLAIANEATIFQKAAFAFGYNDLSLHKIAGTAFCIMMAWWFARRRWWWAI
                     PAVIIAALAGGACEVFQKYFSFRSEDMKDWEAHLKGVAVAAPMLIFIFAGRTGLICRD
                     DGIVSVVGSWKKYVRMSGIAGQAVLIGIAFYFWGGLLFDALPRAAYGNIDIIATDYVI
                     ADDHGKFGKDFLFPAVCNQPLYSGSSEFFGYYKNDENMKMFTCFTLSSRKMAYMSFFV
                     GCPPEGTYRLALLGDPFSPCGYLKRNVMFQVLKPDDKLILMDTDSVLKWAAKNKDRFV
                     TAINALRKQYKVIFIHPGPWKKYCSELPTLRNIFPDIPAVGSSRNENWDFRQAVSLFR
                     SDILIHKNQPRIVCLSDSPHIADNFAASLGDYNCVRSVVVKPNYRLSGALKANHRRHL
                     FLQRDNIMAVLDELEGGGISRR"
     gene            complement(3391..3624)
                     /locus_tag="PLK08_07740"
     CDS             complement(3391..3624)
                     /locus_tag="PLK08_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPS53428.1"
                     /translation="MPIYEYTCENCGKDFEKLVSSSAAKPLCPECGSKKVQRRFSTFA
                     AHGGGAAGKCPSASSCPMSEQPGCCGCGCHGGH"
BASE COUNT          879 a          930 c          934 g          990 t
ORIGIN      
        1 cgaacgaaat tctatctggc gttcgagaag ccccatcgtc catatttatt cggcgaaggc
       61 atagctatgg ccgtcacgct cggctggctg tcaggcgtcg tactgctgac tgaaatacat
      121 gtaaattcaa tcatacaact gcctgtcagg gatttctgga cattgattcc aatatgcata
      181 gccctcgctc tgctggcatc actactcata ggcgcgataa tttctctgct gtggaccgcc
      241 atcagcctca ataaatagga attttgataa ctatggcttg aattttagat ttattctatt
      301 gaacattcgt ccaacaggta tcagcaacat ttaacgtttt aatcataacc acatctcaac
      361 acaagagtta gatggcaaaa accaaataac caacagtcga aatttttttt acaaatttgg
      421 ggtatttacc cgacactttt ttcatgtaac atgttatagt ataaagagcc gaggctaaat
      481 atgcctcggc ggctttttgt ggaaacgcag aaagccttta taatcaggac cctttcagta
      541 accacgtcgt tcctccttcg acgtggtaac cacagtggcg ccggttctcc cccggccggc
      601 gctacgaatt tgtcggttct cccccggccg acaaatctac acaggctcgc gttggtttcc
      661 cccggccagc gcgagttttt ttttgtcctt ccacacccaa aacaaaacgc cgcaaaacat
      721 caggccatat ccaaaatagt cggccaattt tttgttgaga atacttttaa ggctttttat
      781 tgtggtacaa tcaaaattga ctttgtcgga tttatgtgta tatctttcat gcggtactat
      841 ttctttcaac aggataacaa ggatatttca gcaaacatgc gcaaggcact gccacgcgat
      901 atcgtcagga tgctcactac atggtcaggg atggccatga tggttgccct tttgccggtc
      961 gggatgatat tctggcatcc tggatatcgg gcctggggaa tcgtagccgt cggagtcatt
     1021 atcgcaatgc tcatctggat gtttcgcggg ctgcatgaac gtaccaacgc ggttgccggc
     1081 aatccgattc attgggtctg gctgagcatg gcggtgtatc tgagcgtcat ccagtttttc
     1141 gggcagtatt gtcccaagat agagacgtcc agcggcatac gtggcttttt aaacatctcg
     1201 gtaatattgc agttgctgac gctttcgctc ggttgcttgc tgtttcagga atttatgtcg
     1261 aaaggccaga ttgccagatg gctgcgcgag atatcggcag tttttatcat tgttctttcg
     1321 ctgtcgctgg atagtctttt ttcgcctgag acgccgcaaa cggcaatgtg gttaaccggc
     1381 tgtaccgggg tgatgatggc ggcggcgaat gccttttccg ccggctccag cgtcgtcgtg
     1441 ggcgtaagcg gaataaaccg gcggcatcag attgcgatat ggggacagtt gattatcgct
     1501 acggcgggat ttttatgcct tctgtcgatg tgtacggcaa aatcattttg tctgtttttc
     1561 gtcataacgg cgacagtggc cggcattgcc tttatatgtc gcaaacggct cgcagcttcc
     1621 gtcaaatttt acatgacgct tggcatatta tggtggctga ttatcatagc cggctgcctc
     1681 ggcttttaca tgtgcggaga cggcgttttt acgttctggg gacatggcga aaaaggcgcc
     1741 agatattttt ggggaatcga tgacggcatt acggttcttt tcaatcttgt cggttttgtc
     1801 ggggtcggcg gcatgttcgc cggcctggtc gccgtgagca tggccgtaat tgtcaaaaat
     1861 gtcagccgaa gcgatggaaa tttctggaaa gccgtactgt ggctgacggc ctgctggctg
     1921 gccaccgccg catggatgtc cggaaaaaca tttttcagcg tcatgcctct gccgataatg
     1981 atgatgtcgt gggggctgct ttttacattt tccgacgtac gggtcagatg gcacagggcg
     2041 tggcgattcg ccctgccgat ggcgttggga ttattctggc tggctatcgc caacgaagcc
     2101 accatatttc agaaagccgc cttcgctttt ggctataatg acctttcgct gcataaaatc
     2161 gccggcacag ccttctgcat aatgatggcc tggtggttcg cacgccgacg atggtggtgg
     2221 gccattccag ccgtcataat cgccgccctc gccggcggag cctgcgaagt attccaaaaa
     2281 tatttttctt ttcgcagcga ggacatgaaa gactgggaag cgcatctgaa aggtgttgcc
     2341 gttgcagcgc cgatgctgat tttcatattt gccggcagaa ccggcctgat atgccgcgac
     2401 gacggaatag tatcggtcgt cggaagctgg aaaaaatacg tccgcatgtc aggaattgcc
     2461 ggccaggcgg ttcttatcgg cattgcgttt tatttctggg gcggcttgct gttcgacgcg
     2521 ctgccgaggg cggcttacgg caatatcgac atcatcgcga ccgactacgt aatagccgac
     2581 gaccacggca aattcggaaa agacttcctg tttcccgccg tatgcaatca gccgctgtac
     2641 tcaggttcgt cagaattttt cggatactac aaaaacgacg aaaacatgaa aatgttcaca
     2701 tgctttacat tgtcttcgcg caaaatggct tacatgagtt tttttgtcgg ctgcccaccg
     2761 gagggcacgt acaggcttgc gttgttgggc gacccgttca gtccatgcgg ttatctgaaa
     2821 cgcaacgtaa tgttccaggt actcaagccc gacgacaaac tgattctgat ggataccgat
     2881 tccgttttga aatgggccgc taaaaataaa gacaggttcg ttacggccat aaatgccctg
     2941 cgtaaacaat acaaggtaat attcatccac ccagggccat ggaaaaaata ttgcagtgaa
     3001 ctgcccacat tgcgcaatat tttccctgac attccggccg tcggttcatc ccgcaatgaa
     3061 aactgggact tccgccaggc ggtcagcctg ttccgttcag acattctcat ccataaaaac
     3121 cagccgcgca tcgtctgcct gtccgacagt ccgcatattg ccgataattt cgccgccagc
     3181 ctgggcgatt acaactgcgt caggtcggtt gtcgtcaagc cgaactaccg cttgagcggc
     3241 gcattgaaag caaaccaccg ccgacatctg tttttacaac gggataatat aatggcagtg
     3301 ctggatgaac tggaaggcgg cggcataagc agacgctgag gcagacggcg gaaatctgtc
     3361 acttttttga gtaaaaattg agaacgccct ctaatggccg ccatggcagc cgcaaccaca
     3421 acatcccggc tgttcactca tgggacacga cgacgcagac ggacattttc cagccgcacc
     3481 gccgccgtga gcggcaaatg tagaaaaacg acgttgaact tttttactgc cgcattcggg
     3541 acacagaggc ttggccgcag aagatgaaac cagcttttca aagtcttttc cgcaattttc
     3601 acacgtatat tcataaatcg gcatcagtca ttctccttgc gtacgacgac tcggattata
     3661 caatattatc ttcgtgatat tagaacctat cccaaaaaca tgggcgtcgc cgaaatcgag
     3721 caattttgct ggc
//
LOCUS       DAONFI010000235         3693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_30503_length_3693_cov_5.385377, whole genome shotgun sequence.
ACCESSION   DAONFI010000235 DAONFI010000000
VERSION     DAONFI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30503_length_3693_cov_5.385377"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="PLK08_07745"
     CDS             complement(<1..1597)
                     /locus_tag="PLK08_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPS53429.1"
                     /translation="MPAHDIFHVHSSENHMDSHALEAQTGRTTLLIVGILLGGLFVFL
                     SYASDWFFSSYTEMDGQNPYSSALALIGAILLGAPIVWHSVEEMVQGHAHMDELVALA
                     LVAAIASRDYKAAGAVAFFLLLANLIETRTALGARASIESLLQLAPKKALRLHDGDET
                     EEVDPSELRPGDMVRVLPGENIPADGLVRNGQSTVNQANITGESLPVDKAIGDEVFSG
                     TNNVSGALDIEVTRAGEDTTLGRVRKLILEAESSRHPIMRLVDRYSGFYTPTILMIAG
                     TVLYFTRDFERAVTMLVVSCPCALVVAVPTAMVAALSCAARLGILIKDVSMLEVAKDL
                     NAFVFDKTGTLTTGELSVTRMMPAPDVDAADLLSTAASAEQNSRHPVARAVVAIAEKA
                     KVAITRPTEFEELTGRGVTAKVEGSAVMVGRRSWLAESGVDFRSMEDEKFAEPEGLST
                     LYVVKDNRLLGWVGLEDRTRGEAREAIEGLHNEGIRELAMITGDRWSVARRVAREMGC
                     TEVQAEALPADKLKLVHQMKDSGY"
     gene            complement(1852..>3693)
                     /gene="leuS"
                     /locus_tag="PLK08_07750"
     CDS             complement(1852..>3693)
                     /gene="leuS"
                     /locus_tag="PLK08_07750"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPS53430.1"
                     /translation="GEDLITVYTTRPDTLFGATYMVLAPEHMLVKKITTAGQRTAVEE
                     YVSKAVNKSDLERTTESKDKTGVFTGAYAVNPVNDKKIPIWVADYVLMGYGTGAIMAV
                     PAHDTRDYDFAAAFDLPVIQVVETSDGTDWKGFTGDGVAVNSGFLDGLPTQAAKKKMT
                     EWLETNGCGTGATNYKLRDWLFSRQRYWGEPFPIVHCPKCGTVALDESQLPLTLPDME
                     DFKPAMSDDPAALPQPPLGKATDWVNTICPSCGEPAKRELNTMPQWAGSCWYYLRYLD
                     PKNSQKFCGSAASDYWMGDDKKGGVDLYVGGAEHAVLHLLYSRFWHKVLFDLGYVKTA
                     EPFRKLFNQGMITSFTYRDSRGICVPTAEVVFKDDGAYHPKTGEKLVESVEKMSKSLR
                     NVVNPDDIIHDFGADTFRLYEMFMGPLDASKPWNTRDVPGVHRFLQRVWRMIRGTDDN
                     PAIMTDEDTCELDKPLNRLIKKVGEDVESMKFNTAIAAMMEFVNACFKAGRVGRKQAE
                     KFLLVLAPFAPHIAEELWAVLGYEKSLAHESFPSFDETMLTENTVELPVQVNGKIRAK
                     ISVSSDADEQTVFEAAIADEKVAAQIGGKAVFKKILIPGRMLNIVVK"
BASE COUNT          845 a         1031 c          901 g          916 t
ORIGIN      
        1 tgtagcccga atctttcatc tggtgcacaa gtttcagttt gtcagccggc agcgcttcag
       61 cctgcacttc cgtacagccc atttcgcggg ctacgcggcg cgcgaccgac cagcggtcgc
      121 cggttatcat ggccaactct ctgatacctt cgttatgaag gccttctatt gcttcccgtg
      181 cttcgccgcg agtgcggtct tcaaggccga cccagcccag cagacgatta tccttgacga
      241 cgtacaacgt gcttaatcct tcaggttcgg cgaatttttc gtcttccatg ctgcggaaat
      301 ccacgccgct ttccgccagc caggaacggc ggccaaccat gacggctgag ccttcaactt
      361 tcgccgttac gcctctgccg gtgagttcct caaactccgt cggacgggtt atggcgactt
      421 tagccttttc ggctatagca accaccgcac gagccaccgg atgacgagaa ttctgctccg
      481 cagaggctgc cgtggaaagc aggtcggctg cgtcaacatc aggagcgggc atcatgcggg
      541 taacgctcag ttcgccggtc gtaagcgtgc cggttttgtc gaacacaaat gcgttcaaat
      601 ctttggcgac ttcgagcatc gagacgtctt ttataagaat gcccagtcgg gcggcacagc
      661 taagcgccgc aaccatagcc gtcggcactg ccaccaccaa cgcacacgga cacgaaacta
      721 ccagcatggt aacagcacgt tcaaaatcgc gggtaaaata taaaaccgtg ccggcaatca
      781 tcaatatcgt tggcgtatag aaaccgctat agcggtcgac cagacgcatt atcggatggc
      841 gggaggattc ggcttccagg ataagtttgc gaacgcggcc cagcgtggtg tcttcgccgg
      901 cacgggttac ttcgatatcc agagcgccgc tgacgttatt cgtgcctgaa aaaacttcgt
      961 cgccgatagc tttatccacg ggcagagatt cgcctgtgat attcgcctgg ttcaccgtac
     1021 tctggccgtt gcgaaccaag ccgtcggccg gaatattttc acccggcaac acgcgaacca
     1081 tatcaccagg ccgcaattcg gaaggatcta cttcttctgt ttcgtcgccg tcatgcaggc
     1141 gcaacgcttt ttttggtgca agctgcaaaa ggctttcaat gctcgcgcgg gcgccgaggg
     1201 cggtacgggt ctctatgagg tttgccagaa gcagaaagaa cgccactgcg ccggcggctt
     1261 tgtaatcgcg ggacgcaatc gccgccacca acgctaacgc aaccaattca tccatatgcg
     1321 cgtggccctg aaccatttct tcaaccgagt gccaaactat cggtgcaccc agcaggatag
     1381 cccctatcaa cgccaacgca ctgctatacg ggttctggcc gtccatttcc gtatagctgg
     1441 aaaaaaacca gtcgctggca taacttaaaa atacaaacag gccgcccaaa agaataccta
     1501 ctatcaataa tgtagtcctg cctgtctgtg cctccaatgc gtgagaatcc atgtgattct
     1561 cggatgagtg aacgtgaaaa atatcgtggg ctggcattca tctactccga ttataacttt
     1621 ttttaaaata atgaattaca gtatataaag cccgctttcg cctgccgcgg aatcagtttt
     1681 cgccaaacgt gaaaacaatt acacttaaaa agtgtacgtt tcgaatctca acaaagttca
     1741 cgaatttttc aaaaaatcag tttgaatttt tttagaacct atcacaaaaa catgggcatc
     1801 gccgaaatcg agcaattttg ctggcagcca aggtttttgg gatagattct attattttac
     1861 cactatattc agcatcctgc cgggtataag gattttttta aagacagcct tgccgccaat
     1921 ctgtgcagca accttttcgt ctgcaatcgc cgcctcaaat accgtctgct catccgcgtc
     1981 gctggaaacg gagattttgg cgcgaatctt accgttcacc tgcaccggca attcaacggt
     2041 attttccgtc agcatcgttt cgtcaaatga cgggaacgat tcgtgcgcca gcgatttttc
     2101 gtagcccagc acagcccaaa gttcctcggc gatatgcgga gcgaacggcg ccaataccag
     2161 cagaaatttt tccgcctgtt ttctgccgac gcggcctgct ttgaaacacg cattcacaaa
     2221 ttccatcatt gcagctatgg cggtgttgaa tttcatactc tcaacatctt cgccgacttt
     2281 ttttatcagc cggttcagcg gtttatccag ctcgcaggtg tcttcatcgg tcatgatagc
     2341 cggattgtcg tccgtacctc gaatcatgcg ccagacgcgt tgcaggaaac ggtgtacgcc
     2401 cggaacatct cgcgtattcc acggtttcga cgcgtcaagc gggcccatga acatttcata
     2461 cagcctgaac gtatcagccc cgaaatcatg aatgatatca tccggattga cgacgttgcg
     2521 gaggcttttg gacatttttt cgacgctctc gacgagtttt tcgccggttt tcggatgata
     2581 cgcgccgtca tccttaaaaa ccacttccgc cgtcggcacg catatgccgc gcgaatcgcg
     2641 atatgtaaaa ctcgtaatca tgccctggtt aaaaagtttc cggaacggtt ctgcggtttt
     2701 gacgtatccc aggtcaaaaa gcaccttgtg ccagaatcga ctatacaaaa ggtgcagtac
     2761 cgcatgttcc gcgccgccga cgtaaaggtc cacgccgcct tttttgtcgt cgcccatcca
     2821 ataatccgac gccgcactcc cgcagaattt ttgggaattt ttcgggtcga gatatcgcag
     2881 atagtaccag caactgccag cccattgcgg catggtattc aattcgcgct ttgccggctc
     2941 gccgcagctt gggcagatgg tattgaccca gtcagtcgcc ttgccgagtg gcggttgcgg
     3001 cagcgcggcg gggtcgtcgc tcatggctgg tttaaaatct tccatatcgg gtaacgtcaa
     3061 tggaagctga ctttcatcaa gtgcgaccgt gccgcatttc gggcagtgaa ctatcgggaa
     3121 gggttcgccc cagtaacgtt ggcggctaaa cagccagtcg cgcagtttgt aatttgtcgc
     3181 tccggtaccg cagccgttcg tttccagcca ttcggtcatt tttttcttgg cggcctgtgt
     3241 tggcagcccg tcgagaaacc cgctatttac cgcaacgccg tctcccgtaa aacccttcca
     3301 gtcagtaccg tcggatgttt cgactacctg tatcacgggt aaatcgaatg cggcggcaaa
     3361 atcatagtcg cgcgtatcgt gcgctggaac tgccattatc gcgcccgtgc cgtagcccat
     3421 aagtacgtaa tcagccaccc atatcggaat ttttttgtcg ttcacaggat tgacggcata
     3481 tgcgccggta aaaacaccgg ttttatcttt ggattccgta gtacgttcga gatcggattt
     3541 gttgacggct tttgagacgt attcttcaac ggcagtacgc tggccggcag tggttatttt
     3601 ttttaccagc atgtgctcag gcgccaatac catataagta gcgccgaaaa gcgtatccgg
     3661 ccgcgtggta tagacagtaa tcaaatcttc acc
//
LOCUS       DAONFI010000236         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_30556_length_3688_cov_4.420039, whole genome shotgun sequence.
ACCESSION   DAONFI010000236 DAONFI010000000
VERSION     DAONFI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30556_length_3688_cov_4.420039"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="PLK08_07755"
     CDS             <1..1469
                     /locus_tag="PLK08_07755"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPS53431.1"
                     /translation="VGHLNMGAGRIVKQEIVRVDEMIADGSLFKTPRLMQAVARCKQQ
                     NKAFHLMGLVQDQGVHAMGEHFSAFIKFLGSQGVKKVFVHFFADGRDTPPRSALIYLK
                     QLEDVMKQCNCGTVATVMGRFWAMDRAENWARTQKAFNLLTRGEADYTAPSAAAAIES
                     AYKRADEQIAAGKSNPETDEFICPTAIVDSAGQPVGTIQPGDAVLHMNYRQDRAIQLT
                     KAFVEDDFKGFDRGPKPDVFYMGLTRYYDEFPFELIPPMNMANLLGEVLAANRLRQLR
                     IAEFQKYKHVTSFFNGKRLEPLSGEDRIQVESITIPEDKQPEMSAYPVTDLVLKAVND
                     GIAAVRRAAQTTAGVQLELGAELEGACPLNETYDVIVLNYANGDMVGHTGSFDAARKA
                     IETVDECIGKVVPAVLARGGKVLVTSDHGNAEQMLDENGKVQTAHTVSPVHCVLVGND
                     VENVRLISHGKLADIAPTMLKLLGLKIPAEMTAQCLIK"
     gene            complement(1746..2573)
                     /locus_tag="PLK08_07760"
     CDS             complement(1746..2573)
                     /locus_tag="PLK08_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53432.1"
                     /translation="MLNMGRMCVGLVLLSLVAMAQAGSYTQNFNSIAEGSSNLGDGST
                     IVSYVGDEINVTNVYYNGTSWRALRMTEDGVGETQAAYAMPQLDTGSTVTEFTVTFSL
                     LITYNGGGEEWDVPADGFSFNFGTVNLDSLQGMECERGLYTGEGEMLSVSWETFTYAV
                     PSIRVLVNGEEIASEEDYLPIIEDSIDGASFQSVTIHWDSNGLDVTYGANTIYTDLDV
                     SGFAPTEGDIFAFAARTGACYENVFLDNVSIITMPEPLTIGLLATCSLTLLQRKNRK"
     gene            complement(2866..3300)
                     /locus_tag="PLK08_07765"
     CDS             complement(2866..3300)
                     /locus_tag="PLK08_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPS53433.1"
                     /translation="MRLILCLMAAGVLACGGCKAARPATVTIGENTWNVELAITPEQQ
                     YKGLSGRYEIAPDGGMLFINTKEGILRFCMRDCHVPIDIVFINSAMEVVNVYEMQVEE
                     DCSGNITYSSHLPALYALELAGGTIRRDGIKIGDKVKISMPQ"
     gene            complement(3312..>3688)
                     /locus_tag="PLK08_07770"
     CDS             complement(3312..>3688)
                     /locus_tag="PLK08_07770"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPS53434.1"
                     /translation="YATVLAADTIVAAAGEIIGKPADADDARHILRILCRHRHAVVTS
                     LAIISPSCERIIISVATWVKMRLLDEREIENYIESGEWRGKAGAYGIQGAADAFVEKI
                     DGSYTNVIGLPMEAVKKFIPLD"
BASE COUNT          980 a          979 c          866 g          863 t
ORIGIN      
        1 aagtaggcca cctgaatatg ggcgccggcc ggatagtcaa acaggaaatc gttcgcgtcg
       61 atgaaatgat tgccgacggc agccttttca aaactcccag actgatgcag gccgtcgccc
      121 gttgcaaaca acagaacaag gcattccacc tgatgggcct cgtgcaggat cagggcgtac
      181 atgccatggg tgaacatttt tccgcattta taaaatttct gggcagccag ggcgtaaaaa
      241 aagttttcgt acactttttc gccgacggca gagatacccc gccgcgcagc gctctgattt
      301 atctcaagca gctggaagat gtgatgaagc agtgcaactg cggaacggta gcgaccgtca
      361 tgggccgatt ctgggctatg gacagggcag agaactgggc tcgcacgcaa aaagcgttca
      421 atctgctcac cagaggcgag gctgattaca ccgctccatc cgccgctgcc gcaatcgaat
      481 cagcctataa acgtgccgac gaacagatag cagccggcaa aagcaatccg gaaaccgacg
      541 aattcatctg cccgacggcc atcgtcgatt cagccggcca acctgtcggc acaattcagc
      601 caggcgacgc cgtattacat atgaactatc gtcaggacag ggccatacag ttgaccaagg
      661 cgtttgtgga ggacgacttc aaaggctttg accgcgggcc taaaccggac gtcttctaca
      721 tgggtctgac gcgatattat gacgaattcc cattcgagct cattccgccc atgaacatgg
      781 ctaacctgct cggcgaggta ctcgccgcca acaggctgcg acagttacgc atagcggaat
      841 tccagaagta taagcacgtc acgagtttct ttaacggcaa acggctcgaa cctctgtccg
      901 gcgaagaccg catacaggtg gagtccatta cgataccgga agacaaacag cctgaaatga
      961 gcgcgtatcc ggtgaccgac cttgtcctga aagccgtcaa cgacggcata gctgccgtac
     1021 gccgtgccgc ccaaacgact gccggagtcc agcttgaact cggcgcggaa ctcgagggcg
     1081 catgcccgct gaatgaaacg tatgacgtca tcgtcctcaa ctacgccaac ggcgacatgg
     1141 tcggccatac agggtcattc gacgcggctc gtaaagccat cgaaactgtc gatgaatgca
     1201 tcggcaaggt agtgccggct gtattggcca ggggcggcaa ggtgctggta acttcagatc
     1261 acggcaatgc cgaacagatg ctcgacgaaa acggcaaagt gcagacggcc cataccgtca
     1321 gtccggttca ctgcgtgttg gtcggcaacg acgtcgaaaa tgtcagactt atatcgcacg
     1381 gcaaactcgc ggatatcgct ccgacgatgc tgaaactgct gggcctgaaa attccggcag
     1441 aaatgaccgc acagtgtttg ataaaataga acgattcccc aaaaaagtat tttcatttta
     1501 gagcagttct cgaaagaaaa taattttgaa aattgttcta aaaaccattc tggcaaatca
     1561 aaaaattgct taacgagata acaagaaggt gaatcctgca cgtgacacag gatttacctt
     1621 ttttataaac gactctctat gctaatcaag ccaatttgca ggccttttca caaacagatt
     1681 atgcaaaaca atcaaaatta caaccaatca tcattgttca acttatgatg gaacaacaga
     1741 gatagttatt tgcgattttt tctttgcaac aacgtcaaac tacaggtagc aagcaggccg
     1801 atagtaagcg gttcgggcat ggtaatgata cttacgttgt cgaggaaaac attttcataa
     1861 caggcgcccg ttcttgcggc gaaagcaaaa atatcgccct ccgtcggggc gaagccggaa
     1921 acatcaaggt cggtgtagat ggtattggct ccgtatgtca catccaagcc gttggagtcc
     1981 caatgtatcg tgacgctctg aaacgacgca ccgtctatag aatcttcgat aatcggaaga
     2041 taatcttcct cggatgcaat ctcttcaccg ttgactagaa cccggataga cggaaccgca
     2101 taagtaaaag tttcccagct gactgagagc atctcgcctt ccccggtata taatccgcgt
     2161 tcgcattcca taccctggag agagtccaga ttaaccgtac cgaaattgaa cgaaaatcca
     2221 tcggcaggaa catcccactc ctccccgcca ccattatacg ttatcagcag gctgaacgta
     2281 acggtaaact cggtaaccgt actgccggta tccagctgtg gcatggcgta ggctgcttgt
     2341 gtttccccga ctccatcctc cgtcatgcga agtgcccgcc acgaagtgcc gttatagtaa
     2401 acattcgtga cattaatttc atctcccaca tagctgacga tagtcgaccc gtctcccaaa
     2461 ttgctgctgc cttcggcaat agagttgaag ttctgcgtgt aactgcctgc ctgagccatt
     2521 gcgacaagcg ataacaagac cagacctaca cacatgcgac ccatgttgag cataatgtgt
     2581 ccctctcaaa atatgaccgg ctgccactga tcaaccctta accaattgca gacgatctga
     2641 ttcataatta aaatcaacac gacccggaca taaagcgggt acggcaactt taaaatgaaa
     2701 caagcatcag tcagctgata tgccaaccta tttgcctaag agcttattct tctctctccc
     2761 cagtcacgtt taattataag cagacgaatg ctatatgaaa ggatatttta aaaaattctg
     2821 caacttactg tcgttaaggc gctgttctga taattttgcg taatcttatt gcggcatgga
     2881 tattttgact ttgtcgccta tttttatgcc gtcgcggcgg atagtgccgc cggcaagttc
     2941 caacgcgtac aacgccggca gatgcgaaga atacgtgata ttgcccgagc aatcttcttc
     3001 aacctgcatt tcataaacat tgacaacttc cattgcggaa ttgatgaaca cgatatctat
     3061 cggaacatgg cagtcccgca tgcagaatcg cagtatgccc tctttcgtat tgatgaacag
     3121 catgccgccg tccggcgcta tttcatatct gccgctgagt cccttgtact gctgttctgg
     3181 agttatcgcc agttcgacat tccacgtatt ttcgccaatc gtgacagtcg ccggccgggc
     3241 tgctttgcat ccgccgcatg ccagaactcc agccgccatc aaacatagaa tcaatcgcat
     3301 atattatccc ttcaatcaag cggaatgaat tttttaacgg cttccatcgg caggcctatc
     3361 acatttgtat agctgccgtc tattttttcg acaaaagcgt cggctgcgcc ctgtatgccg
     3421 tatgcaccgg cttttccacg ccattcgccc gattcaatat aattttctat ctcacgttca
     3481 tccagcagac gcattttcac ccatgtggcg actgatatga taatccgttc gcatgacggc
     3541 gatattatcg ccaggctggt tacgactgca tggcgatggc ggcagaggat gcgtaaaata
     3601 tgcctggcat cgtcggcatc agcgggtttg ccgataattt ctccggcggc tgcgactatg
     3661 gtgtctgctg cgagcactgt ggcatacg
//
LOCUS       DAONFI010000237         3685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_30591_length_3685_cov_4.193113, whole genome shotgun sequence.
ACCESSION   DAONFI010000237 DAONFI010000000
VERSION     DAONFI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30591_length_3685_cov_4.193113"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..462)
                     /gene="rpmB"
                     /locus_tag="PLK08_07775"
     CDS             complement(205..462)
                     /gene="rpmB"
                     /locus_tag="PLK08_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPS53435.1"
                     /translation="MPRVCPFTGKRTQTGCNISRRGKAKRLGGVGIKVTSRTKRTFKA
                     NMQRVRAVVDGKIVRLNVSTKAIRDGIIVKPAKRDYTPAEA"
     gene            complement(804..2846)
                     /gene="fusA"
                     /locus_tag="PLK08_07780"
     CDS             complement(804..2846)
                     /gene="fusA"
                     /locus_tag="PLK08_07780"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPS53436.1"
                     /translation="MPASKPADIRNIVLLGHGGSGKTSLAEAMLFAVNATTRKGNVLE
                     GTSILDSSDIEKERQHSIDPAMCYLDYAGKTINIIDAPGYPDFIGGALAAMGGADTAV
                     LVISATAGIEVNTRRMFRAAKAAGLPISIVITKIHGEHVDLEAILKNIEDTFGHTCHP
                     LNLPIKGCSEVIDCFANQQGQSDFGNVARAHEMLIDSVVESDEEMMEAYLNGDEIPAD
                     KLSNAFGQAMVEGTVIPMFFTSARDTVGIKELLDGIVKYFPSPAMFSGTPVTAGMAED
                     AKKIDVVCDAAKPFIGHAFKITSDPYVGKLAWIRVLQGTIKGDTQYHLRDEKKTAKVG
                     HLFKAFGGETREIKSAVAGDIVALAKVEEIRFGDVLHEEAADMYRKTPAMPTPMFSLA
                     VEPKSRNDEQKISEALHRFVDEDPTFATHRDSQTHETVICGIGDLHLRMMLMKMKNRF
                     HIEVNTKPPKIPYKETITAPADGHHRHKKQTGGAGQFGEVYLRIEPLERGAGFEFVSE
                     LFGESIPRQFLPAIQKGVEDVIANGAIAGYPMQDIKVAITDGKHHPVDSKEIAFKTAG
                     KHAFIDAVTKAKPVLLEPIVDMEISVPAANMGDIASDLATRRGRIQGQEIVPGNMAVV
                     RAQAPLAEVMQYNSQLRSITGGQGSYSMELSHYEPVPANVQQQIVAAAVKRHDEDE"
     gene            3162..>3685
                     /locus_tag="PLK08_07785"
     CDS             3162..>3685
                     /locus_tag="PLK08_07785"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPS53437.1"
                     /translation="MLKIEISQKSGKARTGRLLTAHGEVDTPTFMPVGTAGTVKGLTP
                     RQIMETGSQMILGNTYHLMLRPTAQKVAALGGLHRMMGWNGPILTDSGGYQVFSLAHL
                     RRLTADAVYFQSHIDGSQVELSPEMAVDIQQLLGSDVMMQLDECTPADAAKQDVAAAV
                     ERSAVWAGRCKKRW"
BASE COUNT          815 a          952 c          956 g          962 t
ORIGIN      
        1 gttctcatct tgcgttattt tcaagaatcg ctacaaaatc tatcccaaaa acatggggcg
       61 tagacacaga tagcagtatg actgccggcc aacttaagcc aagccataaa taacaaaaaa
      121 acgccgtatc gaatgacacg gcgttctcat ttattgcaat gtattttaat tttcggtgcg
      181 ataaaagatt aatcggcagt ttttttatgc ttccgccggc gtatagtcgc gcttggccgg
      241 tttgacgata ataccgtcac ggatagcctt tgtcgaaaca ttcaaacgaa cgattttgcc
      301 gtcaacaacc gcccgaactc tctgcatgtt ggctttgaac gtgcgtttgg ttctgctggt
      361 gactttgatg ccgacgccgc cgaggcgttt tgctttacca cgacggctta tattgcagcc
      421 ggtctgggta cgttttccgg taaaaggaca tacacgaggc atttccgaac tcctgcatat
      481 tttcaattat taacgattct gcacattggc tttgtacgtt ccacatccat acggaacaga
      541 caaacacaaa tcatatgcaa ctcaactttt aactactaac atcaatttta cggcccgcat
      601 tatccctata aagcgggaga cgggtatttt atgcaaatcc gtccgatact caagattttt
      661 tttataattt tttataaaat atgaatccgg caggaatttg cggttattag cgtatatggc
      721 ctgagcggtg gtaaaaaacg cagggccgac ggtaatgact catcagccct gcgcattttc
      781 agacgcaaat agcctgctat tatttattcg tcttcgtcgt gccgcttcac cgccgcggcg
      841 actatctgtt gctggacgtt agccggtaca ggttcgtaat ggctgagttc catgctgtaa
      901 ctgccctgtc cgccggtaat tgagcgaagc tgactgttgt attgcatgac ttccgccaat
      961 ggcgcctgcg ccctgacgac agccatattg ccgggcacga tttcctggcc ctgaattctg
     1021 ccgcgacggg tggcgaggtc gctggcgata tcgcccatgt tcgccgccgg cacggatatt
     1081 tccatatcaa ctatcggttc aagcagaacg ggtttggctt tcgtcacggc atctatgaag
     1141 gcatgtttgc cggctgtctt gaaagctatt tccttcgagt cgaccggatg atgtttgccg
     1201 tcggtaattg ccaccttgat gtcctgcatc ggataaccgg ctattgcgcc gttggcgatg
     1261 acgtcctcga cgcccttctg aatcgccggc aggaactggc ggggtattga ttcgccgaac
     1321 agttcgctga cgaactcgaa acccgctccg cgttcaagcg gctcgatgcg caggtaaact
     1381 tcgccgaact ggccggcgcc gcctgtctgc tttttgtgac gatgatggcc atcggctgga
     1441 gcggtgatgg tctccttgta tggaattttc ggcggtttgg tgttgacctc gatatgaaag
     1501 cggttcttca tcttcatcag catcatccgc aggtgcaggt cgcctatgcc gcagatgacg
     1561 gtttcgtgag tctgcgaatc acgatgagtg gcgaaggtgg ggtcttcatc cacgaagcgg
     1621 tgaagggctt cggaaatttt ctgttcgtcg ttgcggcttt tgggctcgac agccagcgag
     1681 aacatcggcg tgggcatggc tggagttttg cgatacatgt cggcggcttc ctcgtgcagt
     1741 acgtcgccga agcggatttc ttcgactttc gccaacgcca cgatatcgcc ggcgacggcg
     1801 gatttgattt cgcgggtctc gccgccgaaa gccttgaaaa gatggccgac cttggcggtt
     1861 ttcttttcat cgcgaagatg atactgcgta tcgcccttta tagtgccctg caacacccgt
     1921 atccacgcca gcttgccgac gtatgggtcg gaagttatct tgaatgcgtg gccgatgaac
     1981 ggcttggctg catcgcagac gacgtctatt ttttttgcgt cctcagccat gccagccgtg
     2041 accggcgtac cgctgaacat cgccggcgat gggaaatatt ttacaatgcc gtccaacagt
     2101 tcctttatgc ccacagtatc tcgcgccgac gtaaaaaaca tgggaatcac agtgccctcg
     2161 accatcgcct ggccgaaagc attgctgagt ttgtcggcag gaatctcgtc gccgttgagg
     2221 taggcttcca tcatttcctc gtcgctctcg acaacgctgt ctatgagcat ttcgtgagcc
     2281 ctggcgacat tgccgaagtc gctctggccc tgctggttgg cgaaacagtc tatgacttcg
     2341 ctgcagccct ttatgggaag attcagcgga tgacaggtgt ggccgaacgt gtcttcgata
     2401 tttttcagaa tggcttcgag gtccacgtgt tcgccgtgaa ttttcgtgat tacgattgat
     2461 atcggtaaac cagccgcttt tgcagctctg aacatgcggc gggtattgac ttcaatgcct
     2521 gccgtggcgg agattaccag aacggccgta tccgcgccac ccatcgccgc aagagctccg
     2581 cctatgaagt ccggataccc tggcgcgtcg atgatgttta tggttttgcc ggcataatcc
     2641 agatagcaca tcgccggatc gatggaatgc tgcctttcct tttcaatgtc ggacgaatcg
     2701 agaatgcttg ttccttcaag tacgttcccc ttacgagtcg tggcgtttac tgcaaaaagc
     2761 attgcttcgg caagagaagt tttccccgaa ccaccgtgcc ccaggagtac aatgtttctg
     2821 atgtccgcag gttttgaagc cggcataaca cactctccat tcttccggag aataattggc
     2881 tcaaaaacat ccgccaatat tcttcggacg taaaagccgg ccggccagac gctgtcctcc
     2941 acagaaacaa tttcttacgc ctgcccggca taataatcgt tggcacaagc tgcccatgac
     3001 aaaatcctat agtagtgcct tcaaatatcg caggcaagtt tttttagcac gtttttgaag
     3061 aaacaaaatg atttattttt tcgcagagaa atgttcttgc tttttgtggc tggaaattcg
     3121 atttttagtg tggttctgat acgaaagatt tttttaaaaa aatgctgaaa atagaaatat
     3181 cgcaaaaatc cggcaaagcc cgtaccggca gactgcttac cgcgcacgga gaagttgaca
     3241 cgccgacgtt catgccggtg ggcacagccg gcacggtcaa aggccttacg ccgcgtcaga
     3301 ttatggaaac cggctcacag atgatactcg gcaatacgta tcacctgatg ttgcggccca
     3361 ctgcgcagaa ggtagccgcg cttggcggct tgcacaggat gatgggttgg aacgggccga
     3421 tacttaccga cagcggcggt tatcaggttt tttcgttggc tcatctgcgg cggctgacgg
     3481 ctgacgcggt atattttcag tcacatatcg acggttcgca ggtggagctt tcgcctgaaa
     3541 tggcggtgga catacagcaa ctgctcggct cggacgtaat gatgcagctc gacgaatgta
     3601 cgccggctga cgcggcaaag caggacgtcg cagccgccgt cgagcgttcc gccgtatggg
     3661 ccggccgctg taaaaaacgc tggca
//
LOCUS       DAONFI010000238         3674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_30724_length_3674_cov_4.600442, whole genome shotgun sequence.
ACCESSION   DAONFI010000238 DAONFI010000000
VERSION     DAONFI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30724_length_3674_cov_4.600442"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLK08_07790"
     CDS             complement(<1..315)
                     /locus_tag="PLK08_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPS53438.1"
                     /translation="MTYAQPSEILNLTPAEKEYIKQHPVLKVGADRAWAPIEYQADDG
                     NYYGLAMDYMNIVSRLLDIKMDVVRGLAWPDVLSNLQAGELDVLSAVDNSPERQKYML
                     FTR"
     gene            complement(514..1617)
                     /locus_tag="PLK08_07795"
     CDS             complement(514..1617)
                     /locus_tag="PLK08_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS53439.1"
                     /translation="MMGRQSIAIVDDTPANLKLLENMLREQGYRVLAFPRGDFALRGF
                     ERNQPDLILLDINMPGIDGFELCRKLKENEKLRDIPVIFISAYNETIDKVKAFSVGGV
                     DYVTKPFQFEEVIARITTHLKISNLQRELARKFKEQKELEELKDNLIHMIVHDMRSPL
                     QGMLGYLELLQNDSALLGETHRDYVNMLEQSGKMLINMISDLLDVTRLEKNNMPISRQ
                     LCDMRTIIEQSIKNLGYLAENRIDALIPESKVMLMCDRELITRVVVNIVGNALKYEPV
                     GMKVRIACSQSENNVSVSIADDGPGIPEEYHQKIFEKFGQLSADGGRKPLSSGLGLTF
                     ARLAVEAHGGNIALTSEMNKGSTFTFELPKGYE"
     gene            complement(1748..2617)
                     /locus_tag="PLK08_07800"
     CDS             complement(1748..2617)
                     /locus_tag="PLK08_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS53440.1"
                     /translation="MRKFHITSYISGNNVFHLHRANIEHYRYDAGHFHDYDEVFWVEA
                     GEIWHNVNGIEEKLSPGDVCWVKASDRHSFRAAIPNSAIINLLFSRKVLQEIERKYFT
                     GVTTAPWRMPLNHAVNHLTNKQLQLVNHQASQLAFAPQTRLSLDCFLLELIQMLVAGS
                     HLLLPYDEYPIWLGRALRKFVEQGLWDVSFDNFAKMTGRTREHVSRTVKKITGQTVSE
                     LLNSIKLEKAATDLCMTTESILYVAMNAGFNNMSHFYKLFRLRFGQSPKKFRNTRIAI
                     MQLKQPSVNKFIR"
     gene            2802..>3674
                     /locus_tag="PLK08_07805"
     CDS             2802..>3674
                     /locus_tag="PLK08_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HPS53441.1"
                     /translation="MDFFNQSRDVVRRLESEIEFAELFAKLQKKNTWDKMVIKAAAVL
                     AAAVKTENLKELKKAAGEIESLLAPVGKIAKKYTCWCVGHGHMDMNWMWNWAETVAAT
                     NDMFITVLKLMEEFPEFRFSQSQTSVYEIARKYNPELFARIKQRVAEGRWEITASEWV
                     EGDKNLASGESLVRHMLYTRRYMAEHFGLTPDDVSIAWNPDTFGHAHTIPMIYTRGGV
                     QRYYMCRGGESPKPPVFWWSGPDGSRLLVNLEHTWYNDTLGPHNIKGLLSFCEKTGLK
                     DWMLVYGIGDHGGGP"
BASE COUNT          908 a          831 c          893 g         1042 t
ORIGIN      
        1 tcgcgtgaag agcatgtatt tttgtcgttc tggcgagttg tctacagcgg agagcacatc
       61 caattcgccg gcttgcagat tcgaaagcac atccggccat gcaagacccc tgacgacatc
      121 cattttaatg tccaggagcc gcgatacgat attcatataa tccatcgcca agccgtaata
      181 attaccgtcg tcggcttgat attcaatggg cgcccatgcc ctgtcagcgc cgaccttcaa
      241 tactggatgc tgtttgatat attctttttc ggcaggcgtc aggttcagaa tttcggaagg
      301 ctgggcatat gtcaaggtga ttgctagaca aatgcaggct gtcgtcactg tgtgatacca
      361 gcgacttgac agggataaat cttttataaa tcggtaattc aaattttgcc cttttaatat
      421 atccgcggta attttacagt ttcagccgtg taatcttacc gtaattttgc agttttgtga
      481 ttgtaaaatt aatggctgaa cataatttcg ccgttattcg tatcctttgg gcaattcaaa
      541 ggtaaacgta ctgcctttgt tcatttcgct ggttaatgcg atatttcctc cgtgagcttc
      601 tactgccagc cgtgcgaagg tcaggccgag gccggacgat agcggctttc tgccgccgtc
      661 ggcggaaagc tggccgaatt tttcaaatat tttctgatga tattcctcag gtattcccgg
      721 gccgtcgtcg gcaatcgaca ccgatacatt attctcactc tgcgaacagg cgattcgcac
      781 cttcatgcca actggttcat acttcaaagc attgccgacg atattgacga caaccctggt
      841 aatcagttcc ctgtcgcaca tcaacatcac cttgctttcc ggaataagcg catcaatcct
      901 gttttccgcc agatagccga ggttttttat tgactgttca attatggttc tcatatcgca
      961 caattgtcgg ctgatgggca tattattctt ttccagcctg gttacatcga gcaggtcgct
     1021 tatcatgttt ataagcattt tgccgctttg ctccaacatg ttgacgtaat cccgatgggt
     1081 ttcgccgagc agggcactgt cgttttgcaa caattcaagg tatccgagca tgccctgtaa
     1141 cggcgaacgc atatcgtgaa caatcatgtg aataagattg tctttgagtt cttccagttc
     1201 tttttgttct ttgaacttgc gggcgagttc ccgttgcaga ttgcttatct tgagatgagt
     1261 ggtaattctg gcgatgactt cctcgaactg gaagggtttt gtcacataat ctacgccgcc
     1321 gactgaaaat gccttgacct tgtcgatggt ttcgttgtaa gcgcttatga aaatgactgg
     1381 aatatcacgt aatttttcat tttctttgag tttccggcac aattcgaagc cgtcgatgcc
     1441 gggcatattt atgtccagga gaatcaggtc cggctgattt ctttcaaagc cgcgcagcgc
     1501 aaaatccccc ctcggaaatg caaggacacg atatccctgc tccctgagca tgttttccag
     1561 cagtttaaga tttgccggcg tgtcatcgac aatcgcaatg ctttgcctgc ccatcatttt
     1621 tctcccgtaa aacggcaaaa agattttttt gaaattcgtc agccgtccta cataaaagta
     1681 tagcgcctaa cgtattgcga atcaacaact aagccgattt ttccccgctg aaatcagtcg
     1741 tatccgatta tcgtataaat ttattgaccg aaggctgttt tagctgcatg atggcgatgc
     1801 gtgtgtttct gaattttttt ggagattggc cgaagcggag acggaacagc ttgtaaaaat
     1861 gactcatgtt gttaaagccg gcgttcatcg cgacatacaa aatactttcc gtcgtcatgc
     1921 acaggtccgt cgccgctttt tcgagtttta tggaattcaa caattcgctg actgtttgtc
     1981 ccgtaatttt ttttaccgtt ctggagacgt gttcccgtgt acgaccggtc attttggcga
     2041 agttgtcgaa agaaacgtcc cataatccct gttcaacgaa ttttcgcaat gcccggccca
     2101 gccatatggg atattcatca tacggcagca aaagatggct gccggcgacc agcatctgta
     2161 tgagttccag cagaaaacag tccagggaca gtcttgtctg cggcgcgaag gcaagttggg
     2221 atgcctgatg atttaccagt tgcagctgtt tgttcgtaag atgatttacc gcatgattga
     2281 gcggcattcg ccatggggcg gtagtgacgc cagtaaagta tttacgctcg atttcctgca
     2341 gaacttttcg gctgaaaaga agattgatga ttgcggagtt gggaattgcc gcacgaaacg
     2401 aatgacggtc ggatgctttt acccagcaga cgtcgcctgg cgagagtttt tcttctatgc
     2461 cgttgacgtt atgccatatt tcgccggctt cgacccagaa tacttcatcg taatcgtgga
     2521 agtgtccagc atcgtagcgg tagtgctcga tatttgccct gtgcagatga aagacattgt
     2581 ttccggagat gtaactggta atatggaatt ttcgcataat atcaatacag catataaaaa
     2641 tgtgcctaaa aacgcaagaa tatcataatg ccaaaattta taatcatgtc attcctgatt
     2701 aggtgatttt tgaaagtatc ggccaataat taaagcctgt atttgtgaca ggtctttaca
     2761 atcagggcga aaattaaaaa ttttttgtga gagaatcgat tatggacttt tttaatcaga
     2821 gcagagatgt cgtcaggcgt ttggaatcgg aaatagaatt tgcggaactt tttgcaaaac
     2881 ttcagaaaaa aaacacttgg gataaaatgg tcataaaagc tgccgccgtt cttgccgcag
     2941 ccgtgaagac cgagaatctt aaggagttga aaaaggcggc tggggagata gagtcgcttc
     3001 ttgctccggt cggcaaaata gccaagaaat acacctgctg gtgcgtaggc cacggccata
     3061 tggatatgaa ctggatgtgg aattgggctg agaccgtagc cgccaccaac gacatgttca
     3121 taaccgtcct gaagctcatg gaagagtttc ccgaatttcg tttcagccag agtcagacct
     3181 ccgtctacga gattgccaga aaatacaatc ctgagctttt tgccaggata aagcagcgtg
     3241 ttgcggaagg ccgttgggaa atcaccgcca gcgagtgggt ggaaggagac aaaaatcttg
     3301 ccagcggcga aagtctcgtg cgacacatgt tgtatactcg ccggtatatg gcggaacatt
     3361 tcgggctgac gccggatgac gtttctatag cgtggaatcc cgatacgttc ggccacgccc
     3421 ataccattcc gatgatttat actcgcgggg gcgttcagcg ttattatatg tgccgtggcg
     3481 gcgaatctcc caagccgccg gttttctggt ggtcagggcc ggacggcagc aggttgctgg
     3541 tcaaccttga acatacgtgg tacaacgaca cgttgggccc gcataatatc aaggggttgt
     3601 tgtcgttctg tgaaaagacg ggtcttaaag actggatgct cgtttacggc ataggtgacc
     3661 atggcggcgg gccg
//
LOCUS       DAONFI010000239         3666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_30832_length_3666_cov_3.383273, whole genome shotgun sequence.
ACCESSION   DAONFI010000239 DAONFI010000000
VERSION     DAONFI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30832_length_3666_cov_3.383273"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..2237)
                     /gene="glgP"
                     /locus_tag="PLK08_07810"
     CDS             complement(21..2237)
                     /gene="glgP"
                     /locus_tag="PLK08_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPS53442.1"
                     /translation="MRPLRTFTIEPLLPQALEPLAMIAENIWWSWNHDAQDIFRRLDS
                     DLWEATHHNPVAMLGQIDQFRLEEVSKDAGYVAQLQHVAKNLEEYMAAEGWWVQKYGK
                     NEMPEIAYFCAEFGLTECLPIYSGGLGVLAGDHMKSASELDLPLVGVGLLYQQGYFRQ
                     RLNADGWQLELFPRNDFHNLPVELVSGSDGQPLKISVEFPDRNVMAQIWQVIVGRISI
                     FLMDTNVPENMPDDRRITSQLYGGDHEIRVQQEIILGIGGVRMLMALGIQPKAFHMNE
                     GHSAFLTLERIRVIMQEKKISFAEAREAVAASSIFTTHTPVPAGNDAFEPWLVEKYFS
                     HYVSQLGMGVKDLLNMGRLEPFTDDTPLNMTILALRTSCFHNGVSLLHAEVSREMWKK
                     TWPALPKHEVPIDGIVNGIHTHSWVSRDISSLFDRYLGPDWRVRPYDQSVWKRAYNIP
                     DEEIWRTHERRRERLVAFARRHMIQMLSNRGAGKSEIEAASEILDPEALTIGFARRFA
                     TYKRANLLFSDIERLKHILKSSGRRVQIIFAGKAHPKDTPGKELIRQIIHIARDEFLQ
                     NSIVFLEDYDMNVARYLVEGVDVWLNTPRRPMEASGTSGMKAAANGALNLSIPDGWWA
                     EGYSPQVGWSIGSGEMYEDEKYQDEVESRALYDLLEKEVIPLFYDRGKDNLPRQWIAM
                     MKNSIGRLAPLFSTNRMVRQYADMYYKPAMNNWQRLSADEFAGSKELSGWKRKKLT"
     gene            2643..>3666
                     /locus_tag="PLK08_07815"
     CDS             2643..>3666
                     /locus_tag="PLK08_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53443.1"
                     /translation="MQRNNYYNPRGGSDMGHALDRNPLLGSGGRNSGSGSTQYSTYTQ
                     NPDSFTDITRSRGLSNFHGNAPSINDTFYGNSSTFGTNDFDRRAVSVADVVGNPTSIY
                     NPKNAAPFYGQGETTMTLGQVERANILNVAPIPGNSNPTVDIARQNSMNELTNEMNTP
                     QYNRVVSTIMLQNTPYSAQVAASAPGTLPSPTNPQDILELQRALDRVGYSSGSFGVTN
                     PYQQTTLANEISQFDSEEDQINQQNGMVNARIDSNPIASENSVASIQNNPITGKPPVR
                     EDFLYTEQPAAQTASDVQGGIYTAQPSETTTAGFNQDAFSDLRTALQKKAVEERNAKE
                     KQRKEAM"
BASE COUNT          932 a          994 c          875 g          865 t
ORIGIN      
        1 ccttgttcgc cccggcagag tcacgtcaat tttttccgct tccagccgct gagctccttc
       61 gagccggcaa attcgtcggc ggagagccgc tgccagttat tcatagccgg cttgtaatac
      121 atgtcggcat attgccttac catgcggttg gtgctgaaca gcggagccag ccggccaatg
      181 gaattcttca tcatggctat ccactgacgc ggcagattgt ccttgccacg gtcataaaac
      241 agcggaatca cttccttttc cagaaggtcg taaagggcgc ggctttcaac ctcgtcctgg
      301 tatttttcat cttcatacat ttcgcccgat cctatgctcc agccgacttg cggcgaataa
      361 ccctccgccc accagccgtc cggaatcgag agattcagag cgccgttcgc cgcagccttc
      421 atgccgctgg tgccggaggc ctccatcggc cttcgcggcg tgttcagcca cacgtccaca
      481 ccttcgacaa gataacgggc tacgttcatg tcatagtctt cgagaaaaac tatggagttc
      541 tgcaaaaatt catcacgtgc gatatggatt atctggcgga taagttcctt accgggcgta
      601 tctttgggat gagcttttcc cgcaaatatt atctgaacgc gacggccgga tgatttgagg
      661 atgtgcttga gccgttcaat atcgctaaaa agcagattag cccgcttata cgtggcaaac
      721 cggcgggcaa aacctatcgt caaggcttca gggtcgagta tttcggatgc agcctctatt
      781 tcactcttgc cggcgcctcg atttgagagc atctgaatca tatggcggcg ggcgaaggct
      841 accaatcgtt cgcggcggcg ttcgtgcgtc cgccagattt cctcgtcagg gatattgtac
      901 gcccgtttcc agacgctctg atcatacggc cgaactcgcc aatcagggcc gagatagcgg
      961 tcaaacaaac tcgagatatc ccggctgacc catgaatgcg tatgaatgcc gttgactatg
     1021 ccgtcgatag gcacttcatg tttgggcaat gccggccagg tttttttcca catctcgcgg
     1081 gatacttccg catgcagaag gcttacgccg ttatggaagc agctcgtccg cagagccaat
     1141 atggtcatgt taagcggcgt gtcgtcggta aaaggctcca gccggcccat attcagaagg
     1201 tcttttacac ccatgcccag ctggctgaca taatgcgaaa aatatttctc caccagccac
     1261 ggctcaaaag cgtcattgcc ggcaggcact ggcgtatggg tggtgaaaat acttgaggcg
     1321 gcgacggctt cccgcgcctc ggcgaaactt atcttctttt cctgcattat gacgcgtatg
     1381 cgttcaagag tcagaaatgc cgaatggcct tcgttcatgt gaaacgcctt gggctgaatg
     1441 cccaacgcca tgagcattct gacgccgccg atgcccagga tgatttcctg ctgaacccgt
     1501 atttcgtgat cgccgccgta aagctgtgaa gttatgcggc ggtcatcggg catattttcc
     1561 ggcacatttg tgtccataag gaaaatgctt atccggccga caatcacctg ccatatctgg
     1621 gccatgacgt tgcggtccgg gaattccaca gaaatcttca gcggctggcc gtcactgccg
     1681 gaaacaagtt ccaccggaag attgtgaaaa tcattccgcg ggaaaagctc aagctgccat
     1741 ccgtcggcat tgagccgctg acgaaaatag ccctgctgat aaagcagccc gacgccaacc
     1801 agcggcaaat caagttcgga tgcgcttttc atatggtcgc cagccaacac acccaagccg
     1861 cctgaatata tcggcagaca ctcagtaaga ccgaattccg cacaaaagta ggctatctcc
     1921 ggcatttcgt ttttgccgta tttctgtacc caccagcctt cggcagccat gtattcttca
     1981 agattcttgg ctacatgctg aagctgagct acatagccgg cgtctttgga aacctcttca
     2041 agacgaaact gatctatctg gccgagcatg gccacggggt tatggtgagt cgcttcccac
     2101 aaatcggaat caagccttct aaaaatatcc tgagcatcgt gattccacga ccaccagata
     2161 ttttcggcaa tcatggctaa aggctcaagt gcctgcggca gcaacggctc tatggtaaat
     2221 gtccttaatg gtctcatctg ttgccctttt ttgtatcgtg ctccggttaa acgcttgtgt
     2281 tttaacatgg cccgtcttgc acgacaagcc tgcgattcaa acaatctcta aaaacatatt
     2341 gaactatatc attcacggag tttaactgtt actatagatt atagctgatt atatggtaaa
     2401 caatcaaaac tctcttttga gaaaaaacaa aaagtttgtc ggaacaaaaa ctgctttccg
     2461 gcgtcaattt tatagtagcc ttgcggcttt gcagtatata atataataga ggagtttttg
     2521 ggattttgcc aattatagag aataaaacca tgaaaaatat gattagcccg tcgaaagtga
     2581 catttttggc attaattgcc atgttttcat acgcttcggc tcaattgcag ctggatacga
     2641 gagtgcagcg caataattac tataatccca gaggcggctc agacatggga catgccctcg
     2701 accgtaaccc actgttagga agcggcggca gaaacagcgg cagcggctca acgcaatatt
     2761 caacttatac ccagaatccg gattcattta ccgacataac ccgttcgcgc ggcctttcca
     2821 actttcacgg caatgcgcca tcgattaatg acacttttta cggcaattcc agcactttcg
     2881 gaacaaacga ttttgatcgc cgcgccgtga gcgtagctga cgttgtcggc aatccgacat
     2941 caatttacaa tcctaaaaac gcagccccgt tttacggaca gggcgaaacc accatgactc
     3001 tcggccaggt tgaaagagcc aacatcctga atgtcgcccc tatccctggc aacagtaacc
     3061 ccacggtgga tatcgctcgc cagaacagca tgaacgagct cacaaatgaa atgaacacgc
     3121 cgcaatataa tcgcgtcgtt tcgacaatta tgctccagaa cacgccctat agtgcacagg
     3181 tggcagcttc ggcgccaggg actttgcctt cgccgacgaa tccccaggat attctggaat
     3241 tgcaacgggc tctggacagg gtcggctatt catccggctc tttcggcgtt acaaatccgt
     3301 atcagcaaac cacgcttgcc aacgaaatca gtcagttcga cagcgaagaa gaccagatta
     3361 atcaacagaa cggcatggta aacgccagaa tagacagtaa tccgatagca tcggaaaatt
     3421 ccgtagccag cattcagaac aatcccataa ccggcaaacc tccggtcaga gaggacttcc
     3481 tgtataccga acagccagcc gctcaaaccg ccagcgacgt tcagggcggc atatatacgg
     3541 cacagccgtc agaaactacg acggcaggct tcaatcagga tgcgttcagc gatttacgga
     3601 cggcacttca gaagaaggct gtcgaggaac gtaacgccaa ggaaaaacag cgcaaggaag
     3661 ctatgg
//
LOCUS       DAONFI010000240         3653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31005_length_3653_cov_3.529461, whole genome shotgun sequence.
ACCESSION   DAONFI010000240 DAONFI010000000
VERSION     DAONFI010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31005_length_3653_cov_3.529461"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLK08_07820"
     CDS             <1..300
                     /locus_tag="PLK08_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53444.1"
                     /translation="AIRQKISISWAWLCAMTQCFFAAVGGVVVATAVFAPYGFRWPEN
                     LKALEIFNQISFPVFFAIAIIIWAVFVYSMLRARYRWLLRMSGGPSICDSVKSNR"
     gene            complement(385..1731)
                     /locus_tag="PLK08_07825"
     CDS             complement(385..1731)
                     /locus_tag="PLK08_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPS53445.1"
                     /translation="MTPADRQHVLDAIRRKISEEFPAAPGVYLFEDTAGVVLYVGKAK
                     NLKNRAGSYFQPSTNLLQSRGPEISRMVTDLVCDVTFIECDSEVDALLRENRLIKDIQ
                     PRFNERQKDDKSFPYLQIRTAEDFPRVCITRKPESRGVKLYGPFVRGYDLKLALPMLQ
                     RVFKFRTCTLDISECDENRRKYRPCILYNIRQCTAPCAGRVGRDDYRRQIDHLRKFME
                     SKGTQLRRELSEKMQQAAAEKNYEKAAEYRDELKSLQSLQARGLADENVQPEVFYVNP
                     ADGLQKLAEILKLSDAPRTIEGIDIAHLQGKESCGSLVCFIDGKPFKSGYRRYKIKTV
                     DGNDDFASIREIVSRRYRLAGHGEQLFPDVILIDGGKGQLQAAHQAFMRLELQPPMLI
                     SLAKREEEIFISCADEPVRLARNNPALRLLQSVRDEAHRFAQHYHHILRRKATFDE"
     gene            1830..3236
                     /locus_tag="PLK08_07830"
     CDS             1830..3236
                     /locus_tag="PLK08_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53446.1"
                     /translation="MRTLTILSTILMTWTAAMCQDVRYTDDVNGFSLIPPVGSIQTRV
                     DEASVLAQWIKVDPKTGNTEWAFSVSRQVAEVRNTLGEKLKDSEYAALKISSFTESIL
                     SDVQNQPEVKVLSKQEQTIAARQAIVVECAGQVAANLPAEKSAENQRWMKQAWIRVTP
                     RMFLTFKIVAREKFKKRAQTVWQAIMDTLKITDDKAGSVRQQEKAINAEKILDMVVKE
                     KLLGMFSTAAQWFLISRDNIVVGWLCMGAKPQISLETRGYELRSYAEIRIPGQPVKLV
                     YQQMFTSEDLKTEQWRLWMQSGDGAEAKLFAEDGLMQDNHILCETNASGQSPNKQVMD
                     LPTLAVNMYLPKAAGTVIATRLACVGGSGYIFAEYDSARNNLAMRELTVAGPEELSTD
                     TGPIKAVKIVETSDRTALPTLYWVSPEGKILKIQNPAGMVMTSASREMVLQNFPNATA
                     MIDAIRNASQKKWKEGNQ"
     gene            3233..>3653
                     /locus_tag="PLK08_07835"
     CDS             3233..>3653
                     /locus_tag="PLK08_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPS53447.1"
                     /translation="MSEVEVLHDVIIVGAGPAGLAAGLYAARDRYSALLLEKNGLPGG
                     QIMLTEHIENYPGYEKIGGFELVEHMKSQTLRFGARIKTNQAVTSIKRQADGVFLVET
                     NKGENAFRSRAIILAPGSDYRRLGIPGETELRQQGKVS"
BASE COUNT          857 a          912 c         1036 g          848 t
ORIGIN      
        1 gccattcgtc agaagataag catctcgtgg gcatggttgt gcgcgatgac gcagtgtttc
       61 tttgccgcgg tcggcggagt ggtggtggcg acggcggttt ttgcgccgta cggctttaga
      121 tggccggaaa atctcaaagc cctcgaaatt ttcaaccaga tatcgtttcc cgtctttttt
      181 gcgatagcca taatcatatg ggccgttttt gtatattcga tgttgcgtgc gcgttaccgc
      241 tggctgctgc ggatgagcgg cgggccgtcg atatgcgatt cggtaaaatc aaacagataa
      301 attactgcgt gcgtttgtgt atgggatttt gcagtttggt atatgtcggc tgattgtaga
      361 cgaaccggaa aagatgagga gtgtttactc gtcgaatgtg gctttgcggc ggaggatgtg
      421 gtggtaatgt tgtgcgaaac ggtgggcttc gtcgcggacg gattgcagca gccgtaatgc
      481 cggattattg cgagccagcc gcacgggttc atccgcacat gaaatgaaaa tctcctcttc
      541 acgtttagcc agcgatatta gcatgggcgg ctgaagttcc agccgcataa acgcctgatg
      601 tgcagcctga agctgacctt tgccgccgtc gattagtatc acgtccggga aaagctgctc
      661 gccatggccg gccagcctgt agcgacggga aacaatctcc cttatgctcg caaaatcgtc
      721 attgccgtcg acagttttga ttttataccg cctgtaaccg ctcttgaacg gtttgccgtc
      781 gatgaaacaa accagcgagc cgcagctttc tttgccctgg agatgagcga tgtctatgcc
      841 ctctattgtg cgaggggcgt cggacagctt cagaatttca gccagtttct gaaggccgtc
      901 agccggattg acataaaaaa cctccggctg aacgttctca tcggcgaggc cgcgggcctg
      961 gagactttgg agggatttca gttcgtcgcg atactcggcg gctttttcgt agtttttttc
     1021 tgcggcggct tgctgcatct tttctgacag ttccctgcga agttgcgtgc ctttcgactc
     1081 catgaatttc cgcagatggt caatctggcg gcgatagtca tccctgccga cacggcctgc
     1141 acacggcgcc gtgcactgcc ggatgttgta tagtatgcac ggccgatatt tgcggcggtt
     1201 ttcgtcgcat tcggaaatgt ccagagtgca tgtcctgaat ttgaatacgc gctgcagcat
     1261 cggcaacgcc agtttgaggt cgtatccgcg gacgaaaggg ccgtacagtt ttacgccgcg
     1321 cgactcaggc ttgcgggtta tgcagaccct cggaaaatcc tccgccgtgc gtatttgcag
     1381 gtatggaaaa cttttgtcgt ctttctgccg ttcgttgaaa cgcggctgta tgtcttttat
     1441 cagcctgttt tcccgcagca gggcatcgac ctcgctgtcg cattctatga aggtcacgtc
     1501 gcataccagg tcagtaacca tgcgtgaaat ttcagggcct ctggattgca gaagattcgt
     1561 cgacggctga aagtaactgc cggcccgatt tttgaggttt ttggctttgc cgacgtaaag
     1621 tactacgccg gcggtatctt cgaagagata tacgcctggc gcggctggaa actcttccga
     1681 tattttccgg cgaatggcgt cgagaacgtg ttgtctgtct gctggagtca tgccggaata
     1741 atattataac tataacccgt cgggctgtac aacccaaagc ccaatacata taatagcaga
     1801 gagtttttgg ccgtataacg aggacagaca tgagaacgct aacgatactt tcgacgatat
     1861 tgatgacgtg gacggcggca atgtgtcagg acgtccgtta cacggacgac gtgaacggtt
     1921 tttccctcat tccgcccgta gggtcaatcc agaccagggt cgacgaggcg tcggttctgg
     1981 cgcaatggat aaaggtcgat ccgaaaaccg gcaataccga atgggctttt tctgtcagcc
     2041 ggcaggtggc tgaagtccgc aacacgcttg gagaaaaact caaagactcc gaatacgccg
     2101 cactgaaaat atccagcttt acggagtcga tattgtccga cgttcagaat cagcccgaag
     2161 taaaggttct ttcgaaacag gaacagacca tagccgcacg gcaggctatc gtcgtggagt
     2221 gtgccggcca ggttgccgca aatctgccgg cggaaaaatc ggctgaaaat cagcgatgga
     2281 tgaagcaggc ctggattcgc gttacgccga ggatgtttct gacgttcaag atagtggcac
     2341 gcgaaaagtt caagaaacgt gcccagactg tctggcaggc tatcatggac acgctgaaaa
     2401 ttaccgacga caaagccggc tcagtccgcc agcaggaaaa ggctatcaac gcagaaaaaa
     2461 tcctcgatat ggtggtcaag gagaaactgc tgggcatgtt cagcaccgcc gcgcaatggt
     2521 ttttgatatc cagggacaat atcgtcgtag gttggctgtg catgggggct aagccgcaga
     2581 tttcccttga aaccagaggc tatgaacttc gctcttatgc ggagatacgc attcctggcc
     2641 aaccggttaa actggtatac cagcagatgt tcaccagcga agaccttaaa accgagcagt
     2701 ggcgattgtg gatgcagtct ggcgatggtg cggaagcgaa acttttcgcc gaggatggcc
     2761 ttatgcagga caaccacatc ctttgcgaga cgaacgcaag cggccaatct ccgaacaagc
     2821 aggtaatgga tctgccgacg ctggcggtga acatgtatct gcccaaggct gccggtacgg
     2881 tcatcgccac ccgcctggcc tgtgtcggcg gcagcgggta tatttttgcc gaatatgatt
     2941 cagcccgcaa taatctggcc atgcgcgagt tgacagtagc cggccctgaa gagttgtcga
     3001 ctgataccgg cccgataaag gctgtaaaaa tcgtcgaaac cagcgaccgg actgcactgc
     3061 ctacgctgta ctgggtatcg ccggaaggga aaatattgaa aatccagaat ccggccggca
     3121 tggtcatgac ctccgccagc cgcgaaatgg tacttcagaa tttcccgaac gccacagcca
     3181 tgattgacgc aatccgcaac gcatcgcaaa aaaaatggaa agaaggtaat caatgagcga
     3241 agtagaggtt cttcatgatg tgataattgt cggcgcaggg ccggccggct tagccgccgg
     3301 cttgtatgcg gcacgtgacc gctactccgc gctgctgctg gaaaagaacg gcttgccggg
     3361 cggccagata atgctcaccg agcacatcga gaactatcct ggttatgaga aaatcggcgg
     3421 atttgaactc gtggagcata tgaaaagtca gacgctccgg ttcggcgcca ggataaaaac
     3481 caatcaggcg gtcacgtcca taaaaagaca ggctgacggc gtttttctcg tggagaccaa
     3541 caagggtgaa aacgcgttca ggtcacgtgc aataattctt gcgcctggca gcgattacag
     3601 gcggttgggc atccctggcg aaaccgaact ccgccagcag ggcaaagtca gtt
//
LOCUS       DAONFI010000241         3648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31063_length_3648_cov_3.983301, whole genome shotgun sequence.
ACCESSION   DAONFI010000241 DAONFI010000000
VERSION     DAONFI010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31063_length_3648_cov_3.983301"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLK08_07840"
     CDS             <1..670
                     /locus_tag="PLK08_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HPS53448.1"
                     /translation="VAPCTAMVLVWGYLARGNDGLTLVMVAINSLTMLLLYGLLGGFL
                     LGVGQLPVPWQALLLSISIYVALPLVAGYLSRQLMIRTKGLEWFNRKFLHWLTPVTIV
                     ALLITLVLLFSFKGAVILANPLTILWIAVPLFIQTMFIFCLGYWWAKVLRLPYQDAAP
                     AAMIGASNHFEVAIATATMLFGLSSGAALATVVGVLIEVPVMLMLVKICLRTQGWFKL
                     KVKG"
     gene            675..2219
                     /locus_tag="PLK08_07845"
     CDS             675..2219
                     /locus_tag="PLK08_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPS53449.1"
                     /translation="MTDWKTEWEKMFWLAAVFIAVFWLPVNWGRFNSGITESLQLAKW
                     YAREHVLLCLVPAFFIAGAIGVFISQASVMKYLGAKANKVLAYGVAAVSGTILAVCSC
                     TVLPLFAGIYKMGAGLGPATAFLYSGPAINILAIILTARILGLELGIARAVGAITFSV
                     VIGLMMHFIYRKEEVAKADSQLAMPEPEVSRPLWQNSIFFFIMVAILVFVNWGAPNNY
                     EFVLNNGQTFKAAITKSPEDISATGATYRVKILDGRDMGKEMDVATTSISSKNPIPGL
                     WTAVWKYKWQISSVFCIALGLVLIFWFAVAWWKVLFAAIPPAVLALWLPSDGLWVLIP
                     FTAGTVGLSVILSTDKGEGGEWFAQSWGFAKQILPLLFFGVLAAGFLLGRPDHEGFIP
                     GRYVEMLVGDSPNRMLEITGWGGGAFESFARSVWPVWTNLFASIFGAFMYFATLTEVP
                     ILQGLIGAGMGKGPALALLLAGPALSLPSMLVIASVLGARKTIVFVSLVVVMATISGM
                     IYGALF"
     gene            2277..2618
                     /locus_tag="PLK08_07850"
     CDS             2277..2618
                     /locus_tag="PLK08_07850"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPS53450.1"
                     /translation="MFFPCSGSANVGEAADKAARELMHSGKGKMFCLAGLGADIQGMV
                     QAAWDADLNLIIDGCPMGCAKKVFDRHGISNYKCITVTELGIEKAKTRCTDEQVKMIL
                     AKAEEIIEKEA"
     gene            2620..2853
                     /locus_tag="PLK08_07855"
     CDS             2620..2853
                     /locus_tag="PLK08_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPS53451.1"
                     /translation="MKKLQILGTGCPKCKKLTENVEAAAKSLGIEYTIEKVTDINRIM
                     QFGVMMTPALAVDGKVKLVGKVPDVEAIKALLV"
     gene            2868..3338
                     /locus_tag="PLK08_07860"
     CDS             2868..3338
                     /locus_tag="PLK08_07860"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPS53452.1"
                     /translation="MKTKKIISKLLMAFVFISIGFAAGREFTKARMQSQGDITAITSP
                     NADKVVVYYMHASFRCFTCNLVESIGKEIVTKDFADAVKAGRLEWKSVDFQENEKLAQ
                     KYNVGGNMIIVVKFRDGREVDVKRLDKVMALANNRSEFVDYVRPIIQEMLGDKS"
     gene            3335..>3648
                     /locus_tag="PLK08_07865"
     CDS             3335..>3648
                     /locus_tag="PLK08_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPS53453.1"
                     /translation="MTLWMVLATAFWLGLLTSISPCPLATNIAAVSFLARSTGNHRRV
                     LFSGLLYTLGRTIVYVGLGVLILLVFQSAASDGDSGSAAAGISRFLQKYMGVILGPVL
                     ILV"
BASE COUNT          850 a          763 c         1040 g          995 t
ORIGIN      
        1 cgttgcaccg tgtaccgcca tggtgttagt gtggggttat ctggctcgcg gtaacgacgg
       61 actgacgctg gtgatggtgg cgataaattc acttacgatg ctgctgcttt acgggttgct
      121 tggcggattt ctgctgggcg ttgggcagct gcccgtgccg tggcaggcgt tgctgttgtc
      181 catatctata tacgtagcct taccgttggt ggcaggatat ctcagccggc aacttatgat
      241 tcgcaccaaa ggtctggagt ggttcaatcg gaaattcctc cattggctga cgccggtgac
      301 gatagttgcg ttgctcatta cgcttgtgct gttgttcagt ttcaaaggag cagtaatact
      361 ggcgaatccg ctgacaatac tctggatagc cgttccgttg tttatccaga ccatgttcat
      421 attctgcctg ggttactggt gggcgaaagt gctgcgtctg ccgtatcagg atgctgcacc
      481 tgcggcaatg attggcgcgt cgaaccattt tgaggttgcg atagcgacgg caacgatgct
      541 tttcgggctg tcatcaggtg ccgccctggc gacggtcgtg ggtgtgctta ttgaagtacc
      601 agtgatgctc atgctcgtca aaatctgcct gagaacacag ggctggttca aactgaaagt
      661 taagggatag gaaaatgacc gactggaaaa ctgaatggga aaaaatgttc tggctggcgg
      721 ccgtatttat tgcggttttc tggctgccgg tcaactgggg caggttcaac agcggcataa
      781 ccgaatcgtt gcaactggct aaatggtatg cccgtgaaca cgtgttgttg tgtcttgttc
      841 ctgcgttttt cattgccggt gcaataggcg ttttcatttc tcaggcatcg gttatgaaat
      901 acctcggcgc gaaggctaat aaagtattag cctacggcgt agctgccgtg tcgggcacca
      961 ttctggctgt gtgttcatgt accgtgctgc cgctatttgc cggaatatac aagatgggtg
     1021 ctggcctggg gcctgcgacg gcgtttctgt attccggacc tgcaataaat atattggcca
     1081 ttatcctgac cgcgcgaatt ctcggtctgg aactcggtat cgcccgtgca gtcggagcga
     1141 taacgttcag tgtagtcata ggcctgatga tgcactttat ctatcgcaag gaagaggttg
     1201 caaaggctga cagccagctg gctatgccag aaccggaagt atcaagaccg ctgtggcaga
     1261 attcgatatt tttcttcata atggttgcga ttctggtttt tgtgaactgg ggagcgccga
     1321 acaattacga attcgttttg aataacggac aaacctttaa ggcggcgata accaagtcgc
     1381 ctgaagatat ctcggcgaca ggtgcgacat atcgcgtaaa aatcctcgat ggccgggata
     1441 tgggtaagga gatggatgtg gcgacaactt ccatttcgtc caaaaatcct attccgggtt
     1501 tatggacagc agtatggaag tacaagtggc agatttcatc cgtattctgt atagcgttag
     1561 gattggtact tatattctgg tttgccgtgg catggtggaa agttctgttt gcggcgatac
     1621 cgcccgcggt actggccttg tggttgccgt cggatggcct atgggtgctt attccattta
     1681 ctgccggaac ggtcggccta tccgtcattc tcagtaccga taagggcgaa ggcggagaat
     1741 ggttcgctca gtcatgggga tttgccaagc agattctgcc gctgctgttt ttcggcgtgc
     1801 ttgcagctgg ttttttactt ggccggcctg accatgaagg attcattccc ggcagatatg
     1861 tcgagatgct tgtaggtgac agcccgaacc gtatgctgga aatcaccggc tggggcggcg
     1921 gagcgttcga gagttttgca aggtcggtat ggccggtttg gacgaattta tttgccagta
     1981 tattcggcgc tttcatgtat tttgcgacgc tgaccgaagt gccgatactt cagggtctca
     2041 tcggcgctgg tatgggcaag ggccctgcgc ttgcgttact gttggctggc cctgcgctta
     2101 gtctgccgag tatgttggtt atcgcaagcg tacttggtgc gagaaagaca attgtttttg
     2161 tatctttggt ggttgtgatg gccactataa gcggcatgat atacggagca ttgttttagg
     2221 agtgtttaat tatggttgac tgttgctgcg gaaataatga aaaagaaaaa atcgttttgt
     2281 ttttcccctg ttcaggaagt gcaaacgtcg gagaagcagc cgacaaagcc gcccgtgaac
     2341 tgatgcattc cggcaaaggt aaaatgttct gcctggctgg gcttggtgcg gacattcagg
     2401 gaatggtgca ggcggcttgg gatgcggatt tgaacctgat tatcgatggt tgccctatgg
     2461 gttgtgccaa aaaagttttc gataggcatg gtatttcaaa ctacaaatgt attactgtga
     2521 cggaacttgg gattgaaaaa gcaaaaacga gatgcaccga tgaacaggta aaaatgatat
     2581 tggctaaagc tgaagaaatt attgaaaagg aagcttaaaa tgaagaaatt acagattctc
     2641 ggaacaggtt gtcccaagtg caaaaaactt acggaaaatg tggaggcggc agccaagtcg
     2701 ttgggcattg aatacactat tgaaaaagtt accgacatca accggataat gcaattcggc
     2761 gtaatgatga cgccggcatt ggctgtcgac ggcaaagtaa aactcgtcgg caaagtacca
     2821 gatgttgaag caatcaaggc attgctcgta tgaggtattt gacggaaatg aaaactaaaa
     2881 agataatttc aaaactgctg atggcgtttg tattcatcag tatcggcttt gctgccggca
     2941 gggaattcac gaaagcccgc atgcaatcac aaggcgatat aaccgcaatt acatctccga
     3001 acgcagacaa ggtcgtggtt tactacatgc acgcctcgtt cagatgcttc acgtgtaatc
     3061 tcgtcgaatc gataggcaaa gaaatcgtta ccaaagattt cgccgacgcg gtcaaggctg
     3121 gaagacttga atggaagtcg gtggattttc aggaaaatga aaaacttgcc caaaaatata
     3181 acgtcggcgg gaacatgatt atcgttgtga aattccggga cggcagggaa gtggacgtga
     3241 aacggctgga caaggttatg gcgttggcaa ataaccgcag tgaatttgta gattacgttc
     3301 ggcctataat tcaggagatg ctgggagata aatcatgaca ttgtggatgg tgctggctac
     3361 cgccttctgg ctgggtctgc ttacttcaat aagtccgtgt cctctggcga cgaacattgc
     3421 ggcagtgtct tttcttgccc gctctactgg aaatcatcgc agggtgcttt tttccggcct
     3481 tctttatacg cttggccgta ctatcgtgta tgtgggtttg ggagtgttga ttctcttggt
     3541 ctttcagtct gccgcatcgg atggcgattc cggcagtgcg gcggcaggaa tttcccgttt
     3601 tctgcagaaa tacatgggtg taattctggg gccggtattg atactcgt
//
LOCUS       DAONFI010000242         3643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31137_length_3643_cov_3.432553, whole genome shotgun sequence.
ACCESSION   DAONFI010000242 DAONFI010000000
VERSION     DAONFI010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31137_length_3643_cov_3.432553"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLK08_07870"
     CDS             complement(<1..281)
                     /locus_tag="PLK08_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEPR-XLL domain-containing protein"
                     /protein_id="HPS53454.1"
                     /translation="MSYSSNAVKRLQHLYNVIFKRMKFNEWLYEHGLFGRMRNASRPI
                     SALEQLEPRVFLSATTMPTDDLVLHWTADALVAGKLEDTSGYGNAYGTL"
     gene            complement(1154..1630)
                     /locus_tag="PLK08_07875"
     CDS             complement(1154..1630)
                     /locus_tag="PLK08_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPS53455.1"
                     /translation="MMGCDFFHVELLTLRGLVRCSVLFFLELNSRRVHIIPSVVNPTS
                     QWVDQQIKNHYNFDEGIFDNRKYLIHDRDPLFMAKQVQETLRTSGVKSVKIAPCVSNE
                     NAFCESWVATCRREILDKMVFTSKKQLDLALAEFETYYNRWRVHSGLNGNIIDPLP"
     gene            complement(1666..1836)
                     /gene="tnpB"
                     /locus_tag="PLK08_07880"
     CDS             complement(1666..1836)
                     /gene="tnpB"
                     /locus_tag="PLK08_07880"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HPS53456.1"
                     /translation="MLSFPSAVRIYLCREKTDMRKSFDGLSAMVRDIIGADPLSGHLF
                     AVSIPTASIKRS"
     gene            complement(1836..2261)
                     /locus_tag="PLK08_07885"
     CDS             complement(1836..2261)
                     /locus_tag="PLK08_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53457.1"
                     /translation="MAGRTEVYKSKLRLRWQHHIRQWLMSDKLIVDYCREHNIHAGSF
                     YKYKDRLGEEVLAEMMAEQGNDLSGLTLSGAGPARLIKKVRSKSSGRSPFVELKVRPD
                     EESSACIRVELPGGAKMEFPAGIGPEQVGRIICACGGNK"
     gene            complement(2444..3394)
                     /locus_tag="PLK08_07890"
     CDS             complement(2444..3394)
                     /locus_tag="PLK08_07890"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPS53458.1"
                     /translation="MKLGILLPAKPDIKWQIASQAGVKYVVTKAAPELTGQLPPWDFD
                     SLKQTQKRFNEAGFELQALEGDEFDMSRIKLGLPGRDEDIEKYQEMLRNMGRLKIPLL
                     CYNFMAGVGWYRSRTDLKERGGALTSGFFSEDMKEESLKITHEQLWSNYEYFIRSVAP
                     VAEESGVKLGLHPDDPPVSSLCGYPRILTNAAAFRRAIELADSPVAGITFCAATFKVM
                     GENVANLLDEFRHRIWFIHMRDNQPMQGGFRETFHDNGPTDIPSMFELIKKLDINCLL
                     RPDHAPTMAGENNDEPGYAILGRLFAVGYLKGIMDTLKIG"
     gene            complement(3415..>3643)
                     /locus_tag="PLK08_07895"
     CDS             complement(3415..>3643)
                     /locus_tag="PLK08_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPS53459.1"
                     /translation="VILERIASLGRDGYCMNTSYNRWRIDAMSAPLRDSSGEVAAAIT
                     LLGFDDDFTSDKIDGLGKNLKAAVDECATLI"
BASE COUNT          947 a          960 c          703 g         1033 t
ORIGIN      
        1 aatagtgttc cataagcatt accgtaaccg gaagtatctt caagttttcc cgccaccaaa
       61 gcatcggctg tccagtgtag caccagatca tccgtaggca tagttgtagc acttaagaat
      121 actctcggtt caagttgttc cagcgcagaa atcgggcgcg atgcatttct cattcgtcca
      181 aacaggccat gctcatacag ccattcattg aatttcatcc gcttgaaaat gacattgtag
      241 agatgctgta accgcttgac cgcatttgac gaataagaca tgatgtttcc ttcccggagc
      301 cccgaggccc cacaaacgac cgcttcctta aatcaacttc ccccttttca aaaaaagaag
      361 ttgtcgagaa tataccccac ccgtcatccc ataacaatcg attttttctt taaatatttg
      421 taaatacaag cgttatacat atatcgcctt aaaataacat cgggttattt ccttatagca
      481 tttaaggtgt gataaaatcc gttatttcat tgcattttcg ccgtttttgg ctataatatg
      541 gcaaagaggt acatagggta cgaaatctga acctttttta ttgtcaaaat tcttcattta
      601 ccgtcgtaaa ttcatttcaa agaatctctt accgcattcg gggcaactta tctgaaatct
      661 tttactgccg ctttacctga attcacatct caggacagtt ctatccactc tttctatctc
      721 cagaatgcca tcgggtgtcc ttaactaatt tcagttttgg caagtacctt ttgaagatat
      781 atcatatttg ctatatgata tatctttccc atattataat ctgtaataac ctatagttat
      841 tgccctatca ataagtaata catttcgtat tattcattat cttctataag ctattttcaa
      901 catactttct ttgcataaag catattgtcc agcttttcat tcatctttta gctactcgtt
      961 tgtggcacat tttcagttat caattccaca taacatcaag ttaattattt gccagccaat
     1021 tggcctatcg tgaggagtat gtcaaaactc ataactcaca tcaggcagca atatcaaggc
     1081 ttttcttaac tcgtcgatat cctttaagta ttccacctat gaactcttgg caaactattt
     1141 cgccattttc ttcctacggc aacgggtcaa ttatattacc attcagccct gagtgcacgc
     1201 gccatcggtt ataataagtt tcaaactccg ctaacgccaa atctaattgt ttcttggaag
     1261 taaataccat tttatccaat atctcacggc gacaggtcgc cacccaactt tcacaaaaag
     1321 cattttcatt tgaaacacaa ggagcaatct tgacactttt tacgccgctt gttctcagag
     1381 tttcctgcac ttgcttcgcc atgaacagtg ggtctctgtc atgtatcaaa tatttacggt
     1441 tatcgaaaat tccctcgtcg aaattgtaat ggttcttgat ttgttgatct acccactgag
     1501 acgtaggatt cactaccgac ggaatgatat ggaccctacg gctattgagt tccaggaaaa
     1561 acagcacact gcaacgaaca aggcctcgca aggttaaaag ttcgacatga aagaaatcgc
     1621 accccatcat aacatgcata tgcgacttga tgaatttatc ccatgtcagc ttctttttat
     1681 actcgcggtc gggatcgata ccgcgaacag gtgcccgctc agcgggtcgg ccccgattat
     1741 gtcccgcacc atcgccgaca ggccgtcaaa acttttacgc atgtcggtct tttcgcggca
     1801 gagatatatg cgtaccgccg acggaaaact cagcattatt tatttcctcc gcaggcgcaa
     1861 attatcctgc cgacctgctc ggggccaatg cctgccggga attccatctt cgctccgccg
     1921 ggcagttcaa cgcgaatgca tgccgacgat tcctcgtccg gccggacttt caattccacg
     1981 aacgggcttc gaccggaact ttttgaacgt acctttttga taagcctcgc cggccccgcc
     2041 ccagacaacg tcaaccccga aaggtcattg ccctgttctg ccatcatctc cgccagcact
     2101 tcttcaccca ggcgatcttt gtatttgtaa aaactgccag cgtggatatt gtgctcacgg
     2161 cagtaatcga cgatgagctt gtcgctcatc agccactgcc tgatgtgatg ctgccaccgc
     2221 aaacgcaact tgcttttgta aacttccgtc ctgccagcca tgccgcttcg ctcctgataa
     2281 acattatcaa caacgaagcc attatccccg acaaattcca cagctcaagg tgtcatcacc
     2341 ggacgcgtac aacatttgta tattttttac aaaagctatg ataattgcac tgtggttatt
     2401 gcctaaaact aatttactca agttgactga cttttacaga gcactagcca attttcaacg
     2461 tatccataat tcccttgaga tagccgaccg caaacagacg gcctagtatt gcgtagccgg
     2521 gttcgtcgtt attttcgcct gccatcgtcg gcgcatggtc aggacgcagc aggcaattga
     2581 tatccagctt ctttataagc tcaaacatag acggtatgtc ggtaggcccg ttgtcatgaa
     2641 acgtctcacg gaagccgccc tgcatgggtt gattgtcacg catgtgaata aaccatatgc
     2701 gatggcgaaa ttcatccaga aggttggcca cgttttcgcc catgaccttg aaagtggcag
     2761 cacaaaaagt tataccggcc actggactgt cggccagttc gatcgcacga cgaaaggctg
     2821 cggcattagt tagaatacgc ggatacccac acagcgacga gaccggcgga tcgtccgggt
     2881 gaagccccag ttttacgccc gactcctcag ctactggagc aaccgatctg atgaaatatt
     2941 catagttcga ccacaactgc tcgtgagtaa tcttgagaga ttcttccttc atatcttcag
     3001 agaaaaatcc gctggtaagt gccccaccgc gttctttcaa atcagttctg ctgcgatacc
     3061 acccaacgcc agccatgaaa ttatagcaga gcaacggaat tttcaaacgt cccatatttc
     3121 gcagcatttc ctgatatttt tcgatatctt catcgcggcc gggaagtcca agttttattc
     3181 gcgacatgtc gaactcgtcg ccttcgagtg cttgcaattc aaacccagcc tcgttgaaac
     3241 gcttttgggt ttgcttgagc gagtcgaaat cccacggcgg caactggcct gtgagttccg
     3301 gggcagcctt tgtaacgacg tatttcacgc ccgcctgcga agcaatctgc cacttgatat
     3361 caggcttggc aggaagtaat attccgagtt tcataatttg taccacttac attttcaaat
     3421 caatgttgca cattcgtcca cagccgcctt cagatttttc ccaagcccat caattttatc
     3481 cgaggtaaaa tcatcatcga accccagcag ggttatcgcc gccgccactt cgccggaact
     3541 gtctctcagc ggtgcggaca tagcgtcgat acgccagcgg ttgtagctgg tgttcataca
     3601 ataaccgtct ctcccgagcg acgcgatacg ctccaatatt acc
//
LOCUS       DAONFI010000243         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31203_length_3637_cov_3.912619, whole genome shotgun sequence.
ACCESSION   DAONFI010000243 DAONFI010000000
VERSION     DAONFI010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31203_length_3637_cov_3.912619"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..339)
                     /locus_tag="PLK08_07900"
     CDS             complement(115..339)
                     /locus_tag="PLK08_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53460.1"
                     /translation="MNLSISRSIFSVAINPAQSAGITVTTGFTGIRRTAEIVAGFVSA
                     AFLAIAAETQKTKTNSENTCFLNILPLLYP"
     gene            338..2116
                     /locus_tag="PLK08_07905"
     CDS             338..2116
                     /locus_tag="PLK08_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53461.1"
                     /translation="MEFVSATDLGDMMQGMAPSGFMPLFKARFGLGAGFNPEGGFAVV
                     VVNLEKYGFAPDSAKLANFNKMPMVFFIAGRNVKDVFACYAIDEKTHSMKMAKAADVF
                     FFRQVGDYIAISKDKKALDLIDEKNPSIDTVMTASQRKLMAANDMTFFTNCKTVLPYF
                     LNAASDPSVKMDARQRKEIIDEARRNEQIDGLVVGLDVGKNGMSFTLSLEYKKGSELA
                     EMSAAEANKGPVEFNRLPGGRYFMAVSAISLQRAVPGKNNRIISTIMSLFNKVNPDLS
                     YEFQEKFTQILMAAEEQIRDVQFYVGRSDKGLLAISACFNCNSADVLLSLVPQKVDFL
                     REVLHTMVDNGDVKTLTAVADKVAEKTGKPVNTDRLAGVQKIRNIEIDNVPDVAIVEG
                     VTLSAVTMDISNCEISEDASKKILEIFGDEQIRALYGKMDEHTVVVTFGGGVEFAADA
                     IKAAREGGRLDKIPEVAAALKKLPGNTNTVMLINLKNIFDTFNDIKAGCAANTPASKP
                     AATSAPADAATADDESVSGKSDFFTFKAKSPVVISDFCEGNVENIRIDIPMPIFNDII
                     SWFMSEMTKAAMEGKKSNFPLFMPGM"
     gene            complement(2247..>3637)
                     /locus_tag="PLK08_07910"
     CDS             complement(2247..>3637)
                     /locus_tag="PLK08_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPS53462.1"
                     /translation="IEQWQLQRGDVDYDTDGMVVKINELDLRQKLGTTRKYPRWCIAY
                     KYETDRARTKLKSVDFQVGRTGQITPVARFDSVPLGGTRVSNASLHNFDEIERLDVHM
                     GDTVHIEKAGEIIPQVVDVVPAERPYNAPKITPPTLCPSCDSVLQWQSVPEGFIAYRC
                     TEKTCQKFLNRILRKNMPSKCPACGGPVEQVDHLTELLCVNPHCVGILRESILFFAGR
                     NQMDIDTLGPEIVDQLISSGLVKHVTDLYDLTKNELVELERMADKSAQNLMDAIRASK
                     NRGLARLLAALGIRHVGGRAAEILADHFRNIDALMAAPADELQGLTDIGEKIAASVTE
                     YFASKNGRYTIERLKAAGVITTQDISQSTAPQTLDGKTVVVTGTLINFTRQQAQDAIK
                     AAGGRATSSVSASTSFVVAGENPGSKVDDAIKLGVDIIGEDEFIDRLDLKDFMPSKTP
                     DKTPDLPGPLFA"
BASE COUNT          862 a          804 c         1014 g          957 t
ORIGIN      
        1 ccaggctgca aaagagccga ttgcaggcag ccaaggtttt tgggataggt tctagtaagt
       61 gtggtcatat tataatagat tccagattgc caactgttgt tttatgtttg aatatcatgg
      121 ataaaggaga ggtaagatgt ttaaaaaaca tgtgttttca ctattggttt tagttttttg
      181 cgtttccgcg gcaatggcga ggaatgctgc tgagacgaaa ccggcgacaa tttcagcagt
      241 acgccggata ccggtaaaac cggttgttac cgtgattcct gctgattgtg cgggatttat
      301 tgcgactgaa aatattgagc ggctgatgga taaattcatg gaattcgtct cagcgacgga
      361 tttgggcgat atgatgcagg ggatggcgcc gtcgggattt atgcctttat tcaaagccag
      421 gtttggcctt ggcgccgggt tcaatcctga gggcggcttt gccgtggtcg ttgtaaatct
      481 tgaaaaatac ggattcgccc ccgactctgc caagttggct aatttcaaca aaatgccgat
      541 ggtatttttc attgccggca gaaatgtcaa agacgttttt gcttgttatg ccatagatga
      601 aaaaacgcac agcatgaaga tggctaaagc ggctgacgtg ttttttttca ggcaggttgg
      661 cgattatatt gccattagca aggataaaaa agcgttagac cttatagatg aaaaaaatcc
      721 gtcgatagat acggtcatga ccgcatcgca gagaaaattg atggcggcta acgatatgac
      781 gtttttcacc aactgcaaga ctgttttgcc gtattttctc aacgctgcgt cagacccgtc
      841 cgtgaaaatg gacgcacggc agcgaaagga gataattgat gaagcgaggc gtaacgaaca
      901 gatagacgga ttagtcgtcg gccttgacgt tggcaaaaac ggcatgtcgt tcacgttgtc
      961 cctggagtac aaaaaaggtt cggagctggc ggaaatgtcg gctgccgagg cgaacaaggg
     1021 ccctgttgaa ttcaacaggc tgccgggcgg cagatatttt atggcggttt cggctatcag
     1081 cctccagaga gccgtgcccg gcaaaaataa ccggattatc agcacgataa tgagcctgtt
     1141 caataaggtg aatccggatt tgtcttacga gtttcaggaa aaatttacac aaattcttat
     1201 ggcggcagag gagcagatac gggacgtgca attttacgtc ggccggagcg acaagggatt
     1261 gttggccatt tccgcctgtt ttaattgcaa cagtgccgat gtgcttctgt cgcttgtgcc
     1321 gcagaaggtg gattttttgc gggaagtttt acatacgatg gtggataacg gcgacgtgaa
     1381 gacgctcact gccgttgcgg acaaggttgc agaaaaaaca ggcaaaccgg tgaataccga
     1441 cagacttgca ggcgttcaaa aaatcaggaa tatcgaaatt gacaatgtgc cggacgtggc
     1501 aatcgtcgaa ggcgtaacgt tgagtgcggt aacgatggat ataagcaact gcgaaatttc
     1561 cgaagatgcc agcaagaaaa tattggagat ttttggcgac gaacagatac gtgcgctgta
     1621 tggcaaaatg gatgagcata cggttgtagt gactttcggc ggcggagtgg aatttgctgc
     1681 ggacgccatc aaggctgctc gcgaaggtgg caggttggac aaaattcctg aagttgcagc
     1741 cgcattgaaa aagctgcctg gaaacaccaa taccgtgatg ctgataaatc tcaaaaatat
     1801 ctttgacact ttcaatgaca ttaaagccgg ctgtgctgca aatacgcctg cgtcaaagcc
     1861 ggctgcaacc tccgcaccgg ctgatgcggc taccgctgac gatgaatcgg tttccggtaa
     1921 gagtgatttt tttacattta aagcaaaatc gcctgttgtt atatcggatt tttgcgaagg
     1981 caatgtggaa aacattcgca tagacattcc gatgccgata tttaacgaca tcatcagctg
     2041 gtttatgtct gaaatgacca aggcggcgat ggaaggcaaa aaaagcaact ttccgttgtt
     2101 tatgcctggc atgtgaatta aattggattg acagtttttc tttgacgaag acggcgaata
     2161 tgtcggttcg atgcgataaa ttttaacgcc aaagcttggc cttgtgtccg ccggtttttt
     2221 ggacggcttg tgtgaaaaag cggactctat gcgaataggg ggccaggcag gtcgggtgtt
     2281 ttgtccggcg tttttgacgg cataaaatct ttcaggtcga ggcggtcgat aaattcgtct
     2341 tcgccgatta tatcgacgcc gagtttgatt gcatcgtcca cttttgagcc agggttttct
     2401 ccggcgacga cgaagctggt tgatgcggag acgctggaag ttgcgcggcc gccggcggct
     2461 tttattgcgt cctgcgcctg ctgccgcgta aaattaatca gcgtgccggt caccaccacc
     2521 gttttgccgt ccagcgtttg aggtgcggtg ctttgcgaaa tatcctgggt tgtgattacg
     2581 ccggcagctt tgagccgttc gatagtatat ctgccgttct tgctcgcaaa atattcagtg
     2641 acgctggctg cgattttttc gcctatgtcg gtcaagcctt gcagttcatc cgccggcgct
     2701 gccatgagtg cgtcgatatt gcggaaatgg tcagccagta tttcagcggc acgtccgccg
     2761 acgtgacgga tgcccaacgc cgccaatagc cgcgcaaggc cgcggttctt tgatgcccgt
     2821 atggcgtcca tgagattctg cgcagacttg tcagccatcc gttcgagttc gaccagttca
     2881 ttttttgtaa ggtcatacag gtcggttacg tgtttgacga ggccgcttga tatgagctgg
     2941 tcgacaattt ccggcccaag cgtgtcgatg tccatctggt ttctgccggc gaagaacaaa
     3001 atgctttccc gcagtatgcc cacgcagtgc ggatttacac agagcagttc ggtcaggtgg
     3061 tctacctgtt cgaccggccc gccgcaggct gggcattttg agggcatatt tttccgcagt
     3121 atgcggttta aaaatttctg gcaggttttt tccgtacagc ggtaggcgat gaatccctca
     3181 ggtacagact gccattgcag tacggaatcg catgaaggac agagtgtcgg aggggtgatt
     3241 ttcggtgcgt tgtaaggccg ttctgcgggg acgacgtcga cgacctgcgg aatgatttcg
     3301 ccggcttttt cgatgtgtac ggtgtcgccc atatgcacgt ccagccgttc tatttcatcg
     3361 aaattgtgta acgacgcatt cgacacccgc gtgccgccca gcggaactga gtcgaatctc
     3421 gccaccggtg taatctggcc ggtacggccg acctggaaat cgacgctttt gagtttcgtt
     3481 cgcgcgcggt cggtttcata tttgtaggcg atgcaccagc ggggatattt tcgcgtcgtg
     3541 ccgagttttt gacgcaggtc cagttcgttt atttttacga ccatgccgtc ggtatcgtaa
     3601 tcgacgtcac cccgctgcaa ctgccactgt tctatga
//
LOCUS       DAONFI010000244         3617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31453_length_3617_cov_3.397529, whole genome shotgun sequence.
ACCESSION   DAONFI010000244 DAONFI010000000
VERSION     DAONFI010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31453_length_3617_cov_3.397529"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..560
                     /locus_tag="PLK08_07915"
     CDS             54..560
                     /locus_tag="PLK08_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPS53463.1"
                     /translation="MKRAAFFAQVANAMQAMNEAGFVHADMKPNNIIVDDNGNAKIID
                     LGQCCPIGTIKKRIQGTPDYIAPEQVRRFPLDARTDVYNFGASLYWTLTGKPIPTILA
                     KPGSMTVPGQNAMATPPEKLNADVPPLLSRLILDCIRLQPVERPASMREVSMRLGLIL
                     QKLLVPAS"
     gene            557..1396
                     /locus_tag="PLK08_07920"
     CDS             557..1396
                     /locus_tag="PLK08_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPS53464.1"
                     /translation="MNNPAAEIFLNAAELCMNCPQRHGNVVNLSGVGRVMVSGDIHGA
                     RVSLNKIVSRFNSSEQPILILQEIIHGPADAAGFDRSAELLLKAARLKCQHPDRIYFV
                     MGNHDLAQFAGGEITKDGSGVCAAFREGVKNEFGDEAYEVYSAAMKFLRALPLAVRFD
                     NGTLVSHSLPSPSRMKFFDPAVLDNPTLDADIKRGGAVYEMLWGRDQRPEQLDELAKM
                     LGVDFFLLGHRHITAGLLPIPRRAAAINSNALGGCIVEFDAGIDTISLADIEKYVKRI
                     AQL"
     gene            1393..1671
                     /locus_tag="PLK08_07925"
     CDS             1393..1671
                     /locus_tag="PLK08_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HPS53465.1"
                     /translation="MNGLAGKEKRTLRSRGQTLNACVSLGCEGIDEEVVALIERELER
                     SELIKVRLYDETGRQRKETAGRLAEETGSEVVGITGRSVLLFRKAKGC"
     gene            1765..2550
                     /locus_tag="PLK08_07930"
     CDS             1765..2550
                     /locus_tag="PLK08_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53466.1"
                     /translation="MKRYVSLMLLIGLAGLVGCDGDQLNMDYTTPAQKEIGMIYILPG
                     IQGVDYHYKNIRTALIGSGINMAVMIHPWGCQIPGINLMLNETDTRDDRSWGAEIAQG
                     ILQYQQQYPDKPVYMIGQSGGCAVSIFAAETLASLPGAKPLQGIILLDASVSSDYDLT
                     KAMSMCKFGIVNFYNPDDVALLEVGTAVFGNLDGGHGSSAGRVGFSGRYAKLFQVKIK
                     KDMVDDFANPHFADCSKAFTAQYIAPWIIDQEWPPAYITYAKD"
     gene            2588..>3617
                     /locus_tag="PLK08_07935"
     CDS             2588..>3617
                     /locus_tag="PLK08_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPS53467.1"
                     /translation="MPRQGGWELGMRIISVLMIFAVAVGFCGCRKEKPVPAQPLPAVT
                     AARPIQKEVIRHVYFTGRLDSFARVDITARVEGFLQKINFQDGDMVKKGQLLFEIEQD
                     QYSALVEQAQGQLKSVIAQHNDADVNYKRIQYAFKQNVATSQELSDALAKFEVTAAEM
                     ETKDGMLKQAQINLGYTKVYSPIDGMAGKHLVDVGNLVGYGQPTLLVTVIQQDPLYVY
                     FEVSEQNLLLYRKFLRSPKNNKNVEIDHVDLAVRLAGETGYTHKGKLDLTFEGNAVNS
                     STGTITWRGILENRNRLMTPGMFARVQLPIIKMKNTLLVPQVAVGKDLTGNYVFVVGS
                     DNVIEQRHV"
BASE COUNT          906 a          898 c         1009 g          804 t
ORIGIN      
        1 gatggaatat tgccctggcg tgagcgtcgc cgataatcgt ccgaccgaca tacttgaagc
       61 gtgcggcatt ttttgcgcag gtggccaacg cgatgcaggc gatgaatgag gcgggtttcg
      121 ttcatgccga catgaagccc aacaatatca tcgtagacga caacggcaat gccaagataa
      181 tagacctcgg ccaatgctgt cccatcggca cgataaaaaa acgtatccag ggtacgcctg
      241 actatattgc ccctgaacag gtacgccggt ttccgctgga tgcccgtacc gacgtgtaca
      301 atttcggcgc gtcgctctac tggacgctga ctggcaagcc catcccgacc attctggcca
      361 agccaggcag catgaccgtc cccggccaga atgcgatggc gacgccgcct gaaaaactga
      421 atgccgacgt tccgccgctg ctcagcagat tgatattgga ctgcattcgt cttcagccgg
      481 tagaacggcc agccagcatg agagaggtct caatgcggct ggggctgata cttcaaaaac
      541 ttctggtgcc ggcgtcatga acaatccagc ggctgaaatc ttcctcaatg ccgccgaact
      601 ctgcatgaat tgcccgcaga gacacggtaa cgtagtcaat ttatcaggcg tcggcagggt
      661 gatggtttcc ggcgatattc acggggcgcg tgtcagcctc aataaaattg tcagccgttt
      721 caattcgtcc gaacagccga tactcatact ccaggaaata attcacggcc cggctgatgc
      781 agccgggttc gaccgcagtg cggaactgct cctcaaggct gcacgcctga aatgccagca
      841 tcccgaccgt atatatttcg taatgggaaa tcacgatctg gcccagttcg ccggcggaga
      901 aataaccaag gacggcagcg gcgtctgcgc ggcattcagg gaaggcgtaa aaaacgaatt
      961 cggcgatgag gcctacgagg tatattcggc ggcgatgaaa tttttgcgcg ctttgccgct
     1021 tgccgtcagg ttcgataacg gtacgctggt ttcacactcg ctgccgtcgc cctcccgaat
     1081 gaaatttttt gaccctgccg ttttggacaa tcccactctg gatgcggaca tcaaacgcgg
     1141 cggagcggtt tacgaaatgc tgtggggccg cgaccagagg cccgaacagc tcgacgaact
     1201 cgcaaaaatg cttggcgtcg attttttcct gctcggccat cgacatataa ccgccggcct
     1261 tcttccgata ccgcggcggg cggctgcgat aaattccaac gctttgggcg gatgcatagt
     1321 ggaattcgac gccgggattg atacaatatc tctcgctgac atcgaaaaat atgtcaaaag
     1381 gatagcacag ctgtgaacgg ccttgccgga aaagaaaaaa gaactctgcg cagcagaggc
     1441 cagactctca atgcatgcgt atcgctggga tgcgagggca ttgacgaaga ggtcgtcgca
     1501 cttatcgaaa gagaattgga gcggtcggaa cttattaagg taaggctgta cgacgagacc
     1561 ggcaggcaaa gaaaagagac ggccgggcgg ttagccgaag aaaccggctc ggaagtcgtc
     1621 ggtataaccg gcagaagcgt gcttctgttc aggaaagcaa aaggctgctg aggctttttg
     1681 cgtatcgtcg gctcaaaaag ataaagcatg caaaatgttt ttgtaacttt tataaaaatt
     1741 acaagtaaaa cggaggtaac tgagatgaaa cggtatgtat cattgatgtt gttgattggg
     1801 ctggccggcc ttgtcggttg tgacggcgac cagcttaaca tggattacac cacgccggct
     1861 cagaaggaaa tagggatgat atatattctg cccggcattc agggggtcga ttatcattac
     1921 aagaacataa ggacggctct tatcgggtcg ggcataaata tggcagtgat gatacatccc
     1981 tggggctgcc agataccggg tataaacctg atgctcaacg agaccgacac ccgcgacgac
     2041 cgttcgtggg gtgcggaaat agcacagggc atattgcagt atcagcagca atatcccgac
     2101 aagcctgttt acatgattgg ccagtcgggc ggctgtgcgg tgagcatatt tgccgccgag
     2161 actcttgcca gcctgcctgg cgcaaagccg ttgcagggca ttatccttct cgatgcgagc
     2221 gtatcgagcg attacgacct gacgaaggcg atgagcatgt gcaaatttgg catagtgaat
     2281 ttctataatc cggacgacgt tgcattgttg gaagtcggaa cagccgtttt tggcaatctt
     2341 gacggcggtc acggctcgtc agccggccgg gtgggtttta gtggcagata cgccaaactt
     2401 tttcaggtca agataaagaa ggacatggtc gacgattttg ccaatccgca ttttgccgac
     2461 tgttccaagg cgtttacggc ccagtatatt gcgccgtgga taatcgacca ggaatggccg
     2521 ccggcgtata taacttacgc caaggattga aaccgtatag ttttcgggtt gctggcagaa
     2581 ttttcgaatg ccgcggcagg gcggctggga gcttgggatg cgtataatat ccgttttgat
     2641 gatattcgcg gtggccgtcg gcttttgcgg ctgcaggaag gaaaaacccg taccggctca
     2701 gccgttgcct gcagttactg ccgccaggcc tatacaaaaa gaagtaatcc gccatgtcta
     2761 tttcaccggc cgactggatt cttttgcccg cgtggatatt accgcacgtg tcgaaggatt
     2821 tttgcagaaa atcaatttcc aagacggcga tatggtcaag aaaggccaac tcctttttga
     2881 aatcgaacag gatcagtatt cggcattggt cgagcaggct cagggccagc tcaaaagcgt
     2941 aatcgcacag cataatgacg cggacgtgaa ttacaaacgc attcagtatg cttttaagca
     3001 gaatgtcgcc acttctcagg agctttccga tgcactggcc aagtttgagg tgaccgccgc
     3061 cgagatggaa accaaggacg gcatgctcaa acaggcgcag attaacctgg ggtacacgaa
     3121 ggtttattcg cctatagacg gcatggctgg caaacatctt gtagacgtcg gcaatctggt
     3181 gggttacggc cagccgacat tgctggttac ggtaatacag caggatccgc tttacgttta
     3241 ttttgaagtc agcgaacaga atcttcttct ctaccggaaa tttctgcgtt cgccaaaaaa
     3301 caataaaaac gtcgaaattg accacgtaga ccttgcagtg cgtctggctg gcgagaccgg
     3361 ttatacgcac aagggcaagc tcgaccttac gttcgagggc aatgccgtaa attcctcgac
     3421 cggcacgata acatggcgcg gcattctgga gaacaggaac cggctgatga cgcctggcat
     3481 gttcgcccgt gtccaactgc cgataataaa gatgaaaaat acactgcttg tgccgcaggt
     3541 ggctgtcggc aaggatctga cgggcaacta cgtgtttgta gtcggcagtg acaacgtaat
     3601 tgaacagcgg catgttg
//
LOCUS       DAONFI010000245         3607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31595_length_3607_cov_4.375282, whole genome shotgun sequence.
ACCESSION   DAONFI010000245 DAONFI010000000
VERSION     DAONFI010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31595_length_3607_cov_4.375282"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            769..1077
                     /locus_tag="PLK08_07940"
     CDS             769..1077
                     /locus_tag="PLK08_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPS53468.1"
                     /translation="MFNGKILRRWRVKREISMAKLAELVGCSANDISRYENQGRLPRP
                     ARVKKLADVLGIRENELTNSGDVVRDRLEIVLNEMTEVELAKLLADALELLEKRDKNF
                     "
     gene            1244..2422
                     /locus_tag="PLK08_07945"
     CDS             1244..2422
                     /locus_tag="PLK08_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS53469.1"
                     /translation="MKKSDDIGISRRTFDSILAEYCEKYPVFLCAVDICGNVCMADDS
                     LPGRTDDVWRQIRVHAIMESFRWGEPILYDQPAGYIIWAVPLMYNSRMTGGIVACAKE
                     DDVFDEDSRFLFDLTSACRDLFSLAVRENITNDQFLAVHRQIHLREQKRAEAIHDMKS
                     QAARDVLERYLQSEPALVSAIKRGNRGQAVEIINMTLTEIYGLGGDRLDVIKSLLMEL
                     VVTMCRAAIASGGATMELLGNNYSALVELAGIDDEEHLSGWLVQMLDRIMDSISAHLS
                     QKHVADDIERGMNYMEEHYGEDITRGQAADEAGMSESHFARLLKEKTGLSFTDFLNQV
                     RLDRAAELLRKTDRGLMQIALDTGFSDQSYFTRVFRKIMKLTPGQYRTKFRLHDEPRP
                     "
     gene            complement(2478..>3607)
                     /locus_tag="PLK08_07950"
     CDS             complement(2478..>3607)
                     /locus_tag="PLK08_07950"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPS53470.1"
                     /translation="IARIDYASKSTANLRHLQRRAVGAISALADKLLAANNLPKKNLA
                     TVLVGGNTTMQHMLLGVDPSGIAVAPFTPAFTNPREESAANVGLKIAADGKLRVMPAI
                     SGYVGGDIVGDMLAVGWACPDCDRKDTELLIDIGTNGEIVMRAGDEIIACSTAAGPAF
                     EGAKITCGTRAIPGAINSVDVVGDDIAVETIDGKKPLGICGTGLLDIVAVLLELGVID
                     AGGRMAVSAENLPSGVAKRLIPHADGGSAVVLADDVILTARDVREFQLAKAAIAAGVK
                     VLLDLKGRTPANVDAVLLAGAMGSFLRPQNAIRTGLLPYGFKPAQIKAVGNTALEGAG
                     LCLLSGEYRRSAEIISKSCQYVELSGRLDFQTAYMETMLFE"
BASE COUNT          865 a          826 c          938 g          978 t
ORIGIN      
        1 aacgattttt cacggcatat tatcagatta catttatcaa tcatttcagt gtctatgaat
       61 atattgcaag cattcactgg aatgtcgtta ttcatattac tctcttcata attatcaaac
      121 tgcaatcaaa ttataaaaag tacaacctga ctgaaactgg acgagtaatg atattacagg
      181 ctcgactctg tcattggtgt tcttttaccc ttgattttca gcttattccc ttttgttata
      241 aaatataccg agttgtgacg aaatcgcggt ggacactaat ggtttcggga atgatataaa
      301 atcgttacat tcaagtcggg cagagtcagg ttcatttatt tcaaatcaaa cgacaaggct
      361 cagacatcaa aatcgttatc atgtatgagc ttttttaaga ggaaattcct cctttgaaat
      421 cattttttcc acatttattg attgaccacg gcctacacct gctcgcggaa gtttccgcat
      481 gcctttttga cgtatctcag tcattataaa ggcattgata attatatcat cggacacgaa
      541 accctttatc ggaacttata acgtgtatta ttttatacac tttttacaaa ccatttcaag
      601 attatatata ggctttttat ggagttatga ggtatataga agtataaaat atttatttgc
      661 agaatgttat gtgagaaagt tttttcagtt tcatttatag tattcattag tttaaaacga
      721 aacaaggagg ctggtatgtg tgtttcatgt taaaagcatg gagaaagaat gttcaacgga
      781 aaaattctga ggcgttggcg agttaaacgt gaaatcagca tggcgaaact tgctgagttg
      841 gtgggatgca gtgctaacga tatttcgcgt tatgaaaacc agggccgttt accgaggccg
      901 gctcgcgtta aaaaattggc tgatgttttg ggaatcagag agaacgagtt gacaaattcc
      961 ggcgatgtcg tcagggacag gctggaaata gtcctgaatg aaatgacgga agtggagctt
     1021 gccaagctgt tggccgatgc gctggaactt ctcgaaaaac gcgacaaaaa tttttagtgc
     1081 atgttttttt tcgactgtga tattctttat ttgccggcag gcactttttt taagtttgat
     1141 gtattccgtt caatggcaga tgttttggag tggtggggca gaacggcaca tcgtatgaat
     1201 cttttatagc catcgtctta atcttctgtt atcatcttga gtaatgaaaa agagcgacga
     1261 tataggaata agtcgacgga cattcgacag catacttgcc gagtattgcg aaaaatatcc
     1321 tgtttttttg tgcgctgtgg atatttgcgg gaatgtgtgc atggctgacg acagcctgcc
     1381 gggcagaact gatgacgtct ggcggcagat acgcgttcac gcgataatgg aatctttccg
     1441 ttggggcgag ccgattcttt atgaccagcc ggccggttac atcatctggg ccgtaccgct
     1501 tatgtataat tccagaatga ccggcgggat tgtcgcttgc gcgaaagagg acgacgtttt
     1561 tgatgaggac agccggtttc ttttcgacct taccagtgcc tgccgtgatt tattcagcct
     1621 tgccgtgcga gaaaacatca ccaacgatca gtttctcgcc gttcatcggc agattcatct
     1681 tcgcgagcag aagcgggccg aggcgattca cgacatgaaa tcgcaggctg cccgcgatgt
     1741 tctggaaaga tatctccaaa gcgagcctgc tctggtttcg gcgattaaac gcggcaatcg
     1801 cggccaggct gttgagataa tcaacatgac gctgacggag atatatggcc ttggcggcga
     1861 ccggttggac gtgataaaaa gcctgctcat ggaactcgtc gtcaccatgt gccgcgccgc
     1921 catcgcttcc ggcggtgcaa ccatggaact gcttggcaac aattattccg cgctggtcga
     1981 attagccggc atagatgacg aagaacatct ttccggctgg ctggtgcaga tgctcgacag
     2041 aattatggat tcaatctccg cccacctctc gcaaaagcat gttgccgacg atattgaacg
     2101 cggcatgaat tatatggagg aacattacgg cgaagacata acgcggggtc aggctgcgga
     2161 tgaagccggc atgtcggagt cgcactttgc ccggctgctc aaggaaaaaa cggggctgtc
     2221 gtttactgat ttcctgaatc aggtacggct ggacagggct gcggagcttt tgcgaaagac
     2281 cgacaggggg cttatgcaga tagccctgga taccggattt tccgaccaga gctactttac
     2341 gcgggtgttt cgcaaaataa tgaaactcac ccccggccag tatcgtacga aattccggct
     2401 tcacgacgaa ccccggcctt gaacgttgct gttttttaaa aaacgttcca gagcatttca
     2461 aatttaacat gctctaatca ctcaaagagc atcgtttcca tgtaagccgt ctggaaatcg
     2521 agtctgccag ataattcaac gtattgacat gattttgaga ttatttcagc ggagcgtctg
     2581 tattcgccgc tcaggaggca gaggcctgcg ccttcgagtg cggtattgcc gacggctttt
     2641 atctgtgcgg gtttgaatcc gtacggcaga aggccggtgc ggatggcgtt ttgcggacgc
     2701 agaaaacttc ccattgcgcc ggcgagcaat acggcgtcaa cgttagccgg cgtacggcct
     2761 ttgaggtcga gcagcacctt tacgccggcc gctatggctg cctttgcgag ctggaattcc
     2821 cgcacgtcgc gtgccgtgag tatcacgtcg tcagccagca ctaccgccga gccgccgtct
     2881 gcatgcggaa tcaatcgctt ggctacccca gacggcagat tttctgccga tacggccatt
     2941 ctgccgccgg cgtctattac gcccagctcc agcagcacgg ctacgatgtc cagcaagccg
     3001 gtgccgcaga tgccgagcgg ttttttgccg tcgatggttt ctacggctat gtcgtcgccg
     3061 actacgtcga ccgaatttat cgcgcctgga atagctcgcg tgccgcaggt gattttggcg
     3121 ccttcgaatg ccgggcctgc ggctgtcgaa caggcgatta tttcatcgcc ggctcgcatg
     3181 acgatttcgc cgttagtgcc gatgtcgatg agcagttccg tgtcttttct gtcgcaatcc
     3241 gggcaggccc agccgacggc aagcatgtcg ccgacgatat cgccgccgac gtaaccgctg
     3301 attgccggca ttacgcgaag ttttccgtcg gctgctattt tcagaccgac gtttgcggcg
     3361 gattcttcac ggggattggt gaatgccggc gtgaacggtg cgacggctat gcccgatgga
     3421 tctacgccga gcagcatgtg ttgcattgtc gtattgccgc ctacgagtac ggtcgcaaga
     3481 ttttttttag gaagattatt ggcggcaagg agtttgtcgg caagcgcgga aattgcaccg
     3541 acggcgcggc gctgcagatg tcggaggttt gccgttgatt tcgatgcgta atctattctc
     3601 gcgatga
//
LOCUS       DAONFI010000246         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31699_length_3598_cov_4.814564, whole genome shotgun sequence.
ACCESSION   DAONFI010000246 DAONFI010000000
VERSION     DAONFI010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31699_length_3598_cov_4.814564"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLK08_07955"
     CDS             <1..337
                     /locus_tag="PLK08_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53471.1"
                     /translation="AVRIESVHDNIDKSGVCVGGSVKIDAVIVMGNLGIDDLDVELYY
                     GRLDSEGQIENGISLTMMHTENLDNSRHRYLVDMPCGRSGQAGYTVRVVPGRNARCDT
                     FLGDLVRWA"
     gene            complement(353..1819)
                     /gene="gltX"
                     /locus_tag="PLK08_07960"
     CDS             complement(353..1819)
                     /gene="gltX"
                     /locus_tag="PLK08_07960"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPS53472.1"
                     /translation="MTDRIVKTRFAPSPTGSLHVGGVRTALYSYALARKNGGRFLVRI
                     EDTDRARHQEHAVPKILEDLRWLGLNWDEGMEVGGSAGPYRQSERLDIYEKYIQKLLD
                     EGKAYYAFDTEEELEALREKAQQAKQTFRYTTPATLPTAADAKAASAAGRPVVIRLIT
                     ALHDAKFHDEVFGDMLVPAAEQDDFIIRKADGWPTYHMANVIDDALMGVTFICRGQEF
                     LGQTWRHVLLREALGFPEPQYAHLPLIMDMQGRKLSKRDGDVEVESFRKAGFLPEALL
                     NFLALLGWNPGNDIEKFTLAEFCELFSTDRIGKSNAKFDRDKLAAFNADALATVSPER
                     LLACFKDYLSRNDTPIPSGDDGLLRTLLEAHRGMKGFDDIVKKCGVLFGPDDAYTFDE
                     QAIAKNLSKADNAGWTMLETLRGELADVNDFSAQAVEKWIHDYCEKNSLGMGKVAQPL
                     RIAVTGSTVSPGIGVTLALLGKGRTLARIDRCLKIRNG"
     gene            2083..2433
                     /locus_tag="PLK08_07965"
     CDS             2083..2433
                     /locus_tag="PLK08_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPS53473.1"
                     /translation="MLRPIILLVISNIFMTFAWYAHLKNHRTSPLLWVIIASWGIAFL
                     EYCFQVPANRLLKNLDIPLAYLKAIQEIITLVVFSVFSIIYMKEEFRWNYAVGFALLV
                     AAAFFIFYTPKSSA"
     gene            2501..2833
                     /locus_tag="PLK08_07970"
     CDS             2501..2833
                     /locus_tag="PLK08_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HPS53474.1"
                     /translation="MQNAQESCDARPDEQKPVCMIVDITVIDRELYEQYIERVPQTVR
                     EFGGRYYARGGKVVTLAGEWRPERIIMVEFPSMQMLEKWFHSPQYRQIAPLREKSTIS
                     RVIVVELF"
     gene            3081..3326
                     /locus_tag="PLK08_07975"
     CDS             3081..3326
                     /locus_tag="PLK08_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53475.1"
                     /translation="MKTALLKEKLIEISEALNTGDAGCEIDPCEYLDDLTTLKSDMSI
                     DELVDHIRLQSKYLLFALEAAHRERSFLLKMLEKPQG"
BASE COUNT          818 a          864 c          960 g          956 t
ORIGIN      
        1 tgccgtccgt attgaatccg tacacgacaa tatcgacaag tccggcgttt gcgtgggcgg
       61 ctcggtcaag atagatgccg ttatcgtcat gggcaatctg ggcatagatg acctggacgt
      121 ggaactatat tatggtcgtc tggacagcga agggcagatt gaaaacggca tttcgctgac
      181 gatgatgcac acggaaaacc tcgacaattc gcgacatcgt tatctggtgg atatgccgtg
      241 cggccgcagc ggccaggcag ggtataccgt tcgtgtcgtg cccgggcgaa acgcccgctg
      301 cgatacgttc ctgggcgacc ttgtccgctg ggcgtgacat tatttttgtg gattagccgt
      361 ttctgatttt caggcagcgg tcgattctag ccagcgtgcg gcctttgccg agcaatgcca
      421 gcgttacgcc gatgccaggg ctgacagtgc tgccggtaac tgctatacgt agcggctgtg
      481 ccactttgcc catgcccagt gaattctttt cgcagtaatc gtgtatccat ttttcaactg
      541 cctgggcgga aaagtcattt acgtcggcta attcgcctct cagtgtctca agcattgtcc
      601 agccggcgtt gtcggccttt gacagatttt tcgctatagc ctgttcatcg aatgtgtatg
      661 cgtcgtccgg cccgaaaagc acgccgcatt ttttgacaat gtcatcaaac cctttcatgc
      721 cgcggtgtgc ttccagcagc gtccgcagaa gaccgtcgtc gccggaggga atcggcgtgt
      781 cattgcgaga gagataatcc ttgaagcatg cgagcaggcg ttccggactg actgttgcca
      841 gtgcgtccgc gttgaaggcg gctaatttgt cgcggtcgaa cttggcgttg cttttgccga
      901 tacggtctgt gctgaacagt tcgcagaatt cagccagcgt gaatttttcg atgtcgttgc
      961 cgggattcca gccgagcagt gcgagaaaat taagaagcgc ttccggcaga aagccggctt
     1021 tgcggaaact ctcgacctcg acgtcgccgt cgcgtttcga gagcttgcgg ccctgcatgt
     1081 ccatgataag cggcagatgt gcatattgcg gttcggggaa gcccaatgct tctcgcagaa
     1141 gcacgtgacg ccatgtctgt cccaaaaatt cctggccgcg gcagatgaac gttacgccca
     1201 tgagagcgtc gtcgatgaca ttcgccatgt gatacgtcgg ccagccgtcg gctttacgga
     1261 tgatgaaatc gtcctgttcg gcggcaggca cgagcatatc gccgaagacc tcgtcgtgaa
     1321 atttcgcatc gtgcagggcg gttatgaggc ggatgacgac agggcggccg gcggcgctgg
     1381 cggctttcgc atcggcggct gtgggtaacg tggctggtgt ggtgtaacgg aaggtctgtt
     1441 tagcctgttg tgccttttca cggagggctt cgagttcttc ttcggtgtcg aaggcgtaat
     1501 aggctttgcc ttcgtcgagc agtttctgaa tgtatttttc ataaatatca aggcgttcgc
     1561 tctggcgata ggggccggca gagccgccga cttccatgcc ctcgtcccag ttgaggccca
     1621 gccagcggag atcttccagt atcttgggca cggcgtgttc ctgatggcgg gcacggtcgg
     1681 tgtcctctat gcgaaccagg aatctgccgc catttttacg ggcaagggcg taactgtaaa
     1741 gtgctgtgcg aactccacct acgtgaagcg agccggtcgg agacggagca aaacgggtct
     1801 ttacgattct gtcggtcatt tatctatcct taaaatttct tatttcaatg ctctggttac
     1861 aatcgcatga ttgtaatggt cgggtaagta taacgcaacc ttgcgtcgcg gaaaagggcc
     1921 aaaatcggcc gaattgaata tgtagggatg attcggcatc tccgctcttg ttaaatacag
     1981 tcgtcggaat tatacttctt tttcgcaaaa atttgccgcc ggtttatatg atgcaagccg
     2041 ctatcatgac gcgggtttcc gcaggcggac tggagttaaa aaatgttgcg cccgattatt
     2101 cttttggtga tatcgaacat attcatgacg ttcgcatggt atgcccatct taaaaatcat
     2161 cgtacgtcgc cgctgttgtg ggtgataatt gcaagctggg gcattgcgtt tctggagtat
     2221 tgttttcagg tgccggctaa caggctgctg aaaaatcttg atattccgct tgcctatctc
     2281 aaagccatcc aggaaatcat aacgctggtg gttttttcag tattttccat catatatatg
     2341 aaagaggaat ttcgttggaa ttacgcagtg ggatttgcgt tgctggtggc tgccgctttt
     2401 ttcatattct atacgccgaa atcatcggcg tgagtcgtat tggcgatgaa aacacgtcag
     2461 ccggcattac tatcagaaca ttttatggag attatttatc atgcagaatg ctcaagagag
     2521 ttgtgacgcc cggccggacg aacagaagcc tgtttgcatg attgtggaca tcacagtaat
     2581 cgaccgcgag ctttacgagc agtatattga acgtgtacca cagacggtgc gtgaattcgg
     2641 cggcagatat tatgcacgcg gcggaaaggt tgttacgtta gccggcgaat ggcggccgga
     2701 acggattata atggttgaat tcccgtcaat gcagatgttg gaaaaatggt ttcactcgcc
     2761 gcagtaccgc cagatagccc ctcttcgcga aaaatccacc atttccaggg taatagtggt
     2821 tgaattattt tagagcatgt taaacttgat atgcgccatt tctgctggct gcaaaattgc
     2881 ttgatttagg cgacgcccat gtttttggga tttgtgctag aaaatcactc taacactctg
     2941 aaaaacatat aaatcgggcc catatcttag cctgaatcgc cgctgtaatt tttccctaaa
     3001 aaaatctctt tttttcaaac ctctttcggg tgttgagcgt tgaatagata gaaagcatcc
     3061 ggttttgaga ggacatggat atgaaaaccg cattactcaa agaaaagtta atcgaaatct
     3121 ccgaagccct taacaccggc gacgcaggtt gcgaaatcga tccatgcgaa taccttgacg
     3181 atttgacgac gcttaaaagc gacatgtcaa tagatgaact ggtagaccat atccggctgc
     3241 aatccaagta tttgttgttc gcccttgagg cagctcatcg tgagcgttcg ttcctgttga
     3301 agatgctcga aaaacctcag ggataagcaa tagaacgtat ccaccacccg gcagcgctca
     3361 acgtagcccc tgatcgtctg cgtccataat gacgcagacg atcgtttttt tatgccagcc
     3421 ggttttttca cggaatacag atattagaac ctatccccaa aaccttggct gcctgcaatc
     3481 ggctcttttg cagcctggcg gcgtcagaaa aacggcagaa tatttttttt aaaaaaatat
     3541 gccgccattt ttcttccttg ccagcccgta aaattgctcg atttcggcga cgcccatg
//
LOCUS       DAONFI010000247         3593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_31767_length_3593_cov_3.403618, whole genome shotgun sequence.
ACCESSION   DAONFI010000247 DAONFI010000000
VERSION     DAONFI010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31767_length_3593_cov_3.403618"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLK08_07980"
     CDS             <1..334
                     /locus_tag="PLK08_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53476.1"
                     /translation="LNGKVHPVRIEEGESPNWTLAADGLNLCLHTTLSPMQTLWIHGA
                     TSDGRHKAILAPADDYNIQAGIQGHHVNRKDSSDGRWLLIADGMSGRTMSEPLPFVNL
                     DLAGIVLN"
     gene            538..804
                     /locus_tag="PLK08_07985"
     CDS             538..804
                     /locus_tag="PLK08_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53477.1"
                     /translation="MKNVLMLIMLLVICGSGIGTLFTVCGCDDSAAMTVSRGEGVLDD
                     AHQRRRRIQCISDNYARQITDDADEIFFADANLHLNQYLVNYGW"
     gene            complement(1226..2200)
                     /locus_tag="PLK08_07990"
     CDS             complement(1226..2200)
                     /locus_tag="PLK08_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53478.1"
                     /translation="MKHLLTKYIVKMSAVLLLVAIGETAWANDEYDVEDYPAIQPVIE
                     AKNLKLPELWEKNLMPQYRLAVAREVVVAARKMAFNQLVSGVADIKLGDDMTVGSLLA
                     RSDKPDVKPEFFVRGVRENGIIYRQDSLIVEITMEVSRKSILGSIKCWLEVHLSSENQ
                     KYVIKLRDYIESLKGGELRAFGVAAADARYVRPEYAAVQQKAASLVANVPTWVADVVH
                     VRGQGEIKRMTANQQQARDDAFDEATNNARKAMIARVLELKESGDANVGKFIESVPEL
                     TAGLAEIIKSAKITKGTEVIIGDDAEVTVSLDLKKVWNMLLHYRLTGK"
     gene            2489..2926
                     /gene="rplM"
                     /locus_tag="PLK08_07995"
     CDS             2489..2926
                     /gene="rplM"
                     /locus_tag="PLK08_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPS53479.1"
                     /translation="MATKSYMAKKEQQQPKWWVVDASDKIVGRLATKIATILQGKHCP
                     EYTPHVDTGDYVVVVNADKARVTGANKKNQRVYKRFTGYPSGQKLTTMQEAIDSHPER
                     VLEAAVKRMMPKTKLGESMFKKLKVVAGPEHSFQAQQPTKLEL"
     gene            2954..3385
                     /gene="rpsI"
                     /locus_tag="PLK08_08000"
     CDS             2954..3385
                     /gene="rpsI"
                     /locus_tag="PLK08_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPS53480.1"
                     /translation="MSEKTVTSETKSAAAGKYTWGTGRRKASVARVRICQGSGKILVN
                     KRELAAYFPHIKDQFSVNAPLTIAGITSNIDIWANVSGGGNTGQASAIKLGLARAIAK
                     YMPEVERELRDKGLLTRDARSRERKKPGQPGARKRFQFSKR"
BASE COUNT          934 a          942 c          826 g          891 t
ORIGIN      
        1 cctcaacggc aaggttcatc ccgttcgcat cgaagaaggt gaaagtccca actggacgct
       61 tgccgccgac ggcttgaatc tctgcctgca taccaccctc tcgccgatgc aaacgctatg
      121 gatacatggc gcaaccagcg acggcagaca caaggcgata ctggctccgg cggatgatta
      181 caatatccaa gccggcattc aaggccatca cgttaacagg aaagattcgt cggacggcag
      241 atggctgttg atagccgacg gcatgagcgg cagaacaatg agcgagccgc tgccgttcgt
      301 caatctggat cttgccggta ttgtgctgaa ctgacggcaa acacgcagat attgacggca
      361 taacaccgtt ttgaatttga aaatttgact tgtgtgattc ggactagtca gccgataaat
      421 ttattgaatg gtaatatgtg tcgattgtcg gcataatcgg ctgttaaaaa caatgaaatt
      481 tattgcaagg atgctgatta gaattaacgg ctgatgtttt taagaggtga taaaaatatg
      541 aaaaacgtat tgatgctcat aatgctgttg gtaatatgcg gcagcggtat cgggacgctc
      601 tttaccgtct gcggctgcga cgactctgcg gcgatgaccg taagccgcgg cgaaggcgtc
      661 ctcgacgacg cacatcagcg tcgccgccgt attcagtgca tttccgacaa ctacgcccgc
      721 cagattaccg acgacgcgga cgaaatattt tttgcagatg ccaatctgca tttgaaccaa
      781 tacttagtca actacggctg gtaagttgat ttagccactt aacgtaatta ataaaaagca
      841 accccgtccg ataagggcga ggttgttttt ttattcgacg aatcatggac tcgtgaatcc
      901 agtgaatcag ccgaatggtt attttaacag tctgtgcgat aacaaccatc agctcgcatc
      961 tctgacaata gagtggttct ctaaaatggc tcacgatgtt tttactcgta tacctactaa
     1021 tgtagcagag gcaattttca cttattaagt tgaactggta taagaaatgt cggcaccatg
     1081 acaaccatct taaccgtaat gcttccgcaa ctaactacat cggcttacgc aatccatcat
     1141 ccctcgccga ctggccgagc ccgagccgac cattttacgc aatactactc gtcggcatgg
     1201 ccgcccctgc tctactgtat accatctact ttcctgtcaa tctgtaatgc agcagcatgt
     1261 tccagacttt tttgaggtcg aggctgacgg taacttccgc atcatcgccg ataatcactt
     1321 ccgtaccttt ggttatcttc gctgacttta tgatttccgc caggccggct gtgagttccg
     1381 gaacgctttc gataaatttg ccgacgtttg cgtcacctga ttctttgagt tccagcaccc
     1441 tggcaatcat tgccttgcgt gcgttgttcg tcgcttcgtc aaaagcatcg tctcttgcct
     1501 gctgctggtt ggcggtcatt cgttttattt cgccctggcc gcgtacatgc acgacgtcag
     1561 ccacccatgt cggcacattc gccacaaggc tggcggcttt ttgttggacg gctgcatatt
     1621 caggccttac gtagcgggca tctgccgccg ccactccaaa cgccctcaat tcgccgccct
     1681 tgagcgattc tatatagtct cgcagtttta tgacgtattt ctggttttcg ctgctgaggt
     1741 gtacctccag ccagcatttt atactgccca atatgctttt acgtgagact tccatcgtaa
     1801 tctcgacaat cagcgaatcc tgccgataga taatgccgtt ctcacgtacg ccgcgaacga
     1861 aaaattccgg cttgacgtct ggtttatcag acctggccag caggctgccg actgtcatat
     1921 catcgccaag ctttatgtcc gctacgccgc tgacgagttg gttgaacgcc atctttcttg
     1981 ccgcgactac aacctccctc gccacggcga gacgatattg cggcataaga tttttttccc
     2041 ataactccgg caatttcaga ttcttggctt caataaccgg ctggatagcc ggataatctt
     2101 ccacgtcgta ttcatcattc gcccatgccg tttcgccgat agctactaac agcaaaacag
     2161 ccgacatctt cacaatatat tttgtcaaca gatgcttcaa cttagctctc ctcaagtaat
     2221 tacagggaaa atattatacc caaaatccgc acttatgcca ttttttttca tatttgtacc
     2281 tcgtcagccg gcatagccgt aaaggccgct ttcttacagc agccgtccgc cggcagactc
     2341 tcctattttt ttaaaatatg cctctaaaaa cacttgacaa tcgggccctg cgacaccata
     2401 atgcgtgact ctttgtgaga gaggcgtata tttacgcctt tttaaggact ttttacaaga
     2461 ttactgattt taagcaggac cattacagat ggcgactaaa agttatatgg ctaaaaaaga
     2521 gcaacagcag cccaagtggt gggttgtgga tgccagcgat aaaatcgtag gccggctggc
     2581 tacgaagatt gccactattc tccagggcaa acactgcccg gaatacaccc cccacgtgga
     2641 tacgggcgat tacgtggtag tcgtaaatgc ggacaaggcg cgggtaaccg gcgcgaacaa
     2701 gaaaaatcag cgggtataca agcgttttac cggctatccc agcggccaga aactcacgac
     2761 catgcaggaa gcgattgact cgcacccgga acgcgttctg gaagcagccg tcaagcgtat
     2821 gatgccgaaa accaaactcg gcgagtccat gttcaagaag ctcaaggtcg ttgccggccc
     2881 tgaacacagt tttcaggctc agcagccgac gaagctcgaa ctttaaaaat atctgtcaga
     2941 ccaggaaaaa aatatgagcg aaaaaaccgt aacatcggaa acgaagtcag cagccgcagg
     3001 caaatacaca tggggcacag gccgtcgcaa agcgtccgtg gcaagagtac gaatctgcca
     3061 gggcagcggc aaaatactcg tcaacaaacg ggaattagcc gcctactttc cgcacataaa
     3121 ggatcaattt tcggttaatg ccccgctaac cattgccggc ataaccagca acatagacat
     3181 atgggccaat gtttccggcg gcggcaatac cggccaggcc agcgctataa aactcggctt
     3241 agcccgggca atagccaaat acatgcccga agtcgaacgc gaacttcgcg acaagggtct
     3301 gctgacccgc gacgcacgct ccagagaacg taagaaacca gggcagcctg gtgctcgcaa
     3361 acgcttccag ttctcgaaac gttgagcacc gtaccgagcg tggtactttg tcatcaaaaa
     3421 ccccggtatc ttcaggagcc ggggtttttt aataattcaa actgaccgat ttatgatttt
     3481 tcgcatatga ggctaaaagg taaagactta taacgcgaat cggtcacttt gagttaatat
     3541 attatccttc gtaattccaa attcccggat tcaatccggt caataaggat gga
//
LOCUS       DAONFI010000248         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_32366_length_3545_cov_3.752436, whole genome shotgun sequence.
ACCESSION   DAONFI010000248 DAONFI010000000
VERSION     DAONFI010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32366_length_3545_cov_3.752436"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1511)
                     /gene="ffh"
                     /locus_tag="PLK08_08005"
     CDS             complement(81..1511)
                     /gene="ffh"
                     /locus_tag="PLK08_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPS53481.1"
                     /translation="MFEALTDKFSDVFRKMSGRGRLTEENIHEAMREVRTALLEADVH
                     YQVAKKFCDDVAQKAVGTEVLKSLHPSQLMIKIVNDELTALMGPVDTRIYFVSPGPTI
                     IMMAGLQGAGKTTTCGKLANYLIKQGKKPLMVACDLQRPAAVDQLTIVGEQVGVPTFR
                     MEGEKNPVAVARKSINYARGNGNDVVIIDTAGRLHIDEEMMQQAQDIASAVNPHQIYL
                     VCDAMTGQDAVNSAQEFNNRLELDGVILTKFDSDARGGAALSVKAVTGKPLKFIGVGE
                     KFDALEEFYPDRMASRILGMGDVVSLVEKAQEAFDQEQAQKMQEKMAKGRFTLVDFMQ
                     QMKAMQKMGPLKQILKLMPGMGANAAALDMIKGDEFKQLEAIINSMTPKERDEPDTIE
                     ASRRRRIARGAGCQPQDVSGLVKSFGQAANMMKTMANMGIGARMRMASQMAQGGMTGM
                     GKIKQRSKRLSPKERAKRRDKKKKRR"
     gene            1707..2066
                     /locus_tag="PLK08_08010"
     CDS             1707..2066
                     /locus_tag="PLK08_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS53482.1"
                     /translation="MTQDKPRLTIEEVGGVKIVTLSDQRILDELSIAKIGKHLNDLVL
                     QNPKIKIILDFVNVTNMSSSALGMLITLHKRVREAGGELRLCNIQPTIQEVFKITRLD
                     EIFQICVSKGEAIDSVE"
     gene            2123..2533
                     /locus_tag="PLK08_08015"
     CDS             2123..2533
                     /locus_tag="PLK08_08015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPS53483.1"
                     /translation="MVKKQIIKSDYDRARVVEESIISEAQANGFSDVDVFAIRLALEE
                     ALVNAIRHGNRLDPHKKVTVEWCVRNKELEITIEDEGKGFLPNAVPDPTQDENLERPG
                     GRGLMLMKAYMDCVEYNKQGNIVRMCKKLHCCGK"
     gene            2653..3222
                     /locus_tag="PLK08_08020"
     CDS             2653..3222
                     /locus_tag="PLK08_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPS53484.1"
                     /translation="MKAVAFNGSPRVGGNTEFLLKTVLEQISAAGHETEFVQVGGKLL
                     HGCTACLKCRENKNRLCVITNDIVNDCIAKMIEADAILLGSPAYFADMTPEMKALIDR
                     AGYVCSGNGRLLKHKIGAAVVAARRGGATHVLDSINHFFLVSQMIVPGSTYWNFGIGR
                     EKGQAANDSEGVANMQDLGQTIAWLMSRL"
     gene            complement(3300..>3545)
                     /locus_tag="PLK08_08025"
     CDS             complement(3300..>3545)
                     /locus_tag="PLK08_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HPS53485.1"
                     /translation="PDDIKAYDLPTHPLKDVDKKRAQDAMKNDPFIQHYVVWQKAINH
                     MLKLGVRVEQQALAKHGLEFVANTYLPEKLRAGNFLP"
BASE COUNT          817 a          859 c          938 g          931 t
ORIGIN      
        1 tgcaatcaac tcttttgcaa ctcgccgtaa ccgtagatag caggatgact gcatgtcaac
       61 atcaggatgc tctataaaaa ttatctgcgt tttttctttt tatctcgacg tttggcacgt
      121 tcttttggcg agagtcgttt tgaacgctgc ttgatttttc ccatgccagt catgccgccc
      181 tgggccatct gagaggccat ccgcattcgt gcgcctattc ccatattcgc catcgttttc
      241 atcatgttgg cggcttggcc gaaacttttc accaagccgg aaacgtcctg cggctgacag
      301 cctgcacccc tggcgattcg tcgccggcgg gatgcttcga tagtgtccgg ctcatcacgt
      361 tctttgggcg tcatggaatt gataatcgct tcgagctgct tgaattcgtc gccttttatc
      421 atgtccagcg cggcggcgtt tgcacccatg cctggcataa gtttcagaat ctgctttagc
      481 ggccccatct tctgcatggc cttcatctgc tgcatgaaat cgacgagagt aaatcggccc
      541 ttcgccatct tttcctgcat tttttgggcc tgttcctggt cgaaagcctc ctgcgccttt
      601 tcgacgaggc tgaccacgtc gcccatgccg agtatgcggc tggccatgcg gtcggggtaa
      661 aattcctcca gcgcgtcgaa tttctcgcct acgccgatga atttaagcgg tttgccggtg
      721 acggctttca cggacaatgc cgcgccgccg cgggcatcgg aatcgaactt cgtcaggatt
      781 acgccgtcaa gctccagccg gttattgaat tcctgtgccg aattgacggc atcctggccg
      841 gtcatggcat cgcagacaag ataaatctgg tgcggattga cggctgatgc gatgtcctga
      901 gcctgctgca tcatctcttc gtctatgtgc agtcggccgg cagtgtcgat gatgaccacg
      961 tcattgccgt tgccgcgggc gtagtttatt gatttccttg ctacggcgac gggatttttt
     1021 tcgccttcca tgcggaacgt cggcacgcct acctgttcgc cgacgattgt cagctggtcg
     1081 acggctgccg gccgctgaag gtcgcaggca accatgagcg gctttttgcc ctgcttgata
     1141 aggtagttgg ccagcttgcc gcaggtggtg gttttgcctg cgccctgtaa accggccatc
     1201 atgattatcg taggccctgg cgaaacgaaa tatattcgcg tgtcaaccgg ccccatgagc
     1261 gcagtgagtt cgtcattgac gatttttatc atcaactggc tggggtggag ggatttcaac
     1321 acttccgtgc cgacggcttt ttgggcgacg tcgtcgcaga attttttggc tacctggtag
     1381 tgaacgtcgg cttccagcag ggcggtgcgg acttcacgca tggcttcgtg gatattttct
     1441 tccgtcagac ggccgcggcc ggacatttta cggaatacgt cgctgaattt atctgtaagt
     1501 gcttcaaaca tgggccaaag attaccagaa agccgcacat attgcaagaa tgccaatccc
     1561 gtctatcaaa cttttacatc ctgaaaatcc atatcgtcgg ccattacgct tgacacagag
     1621 tttttggggg agtatcatct tataaaacca gtaattatgt gttttggttc aatatgattc
     1681 tgattcgaat ttaaggagtc cagttcatga cgcaggacaa acccagactg accatcgaag
     1741 aggttggcgg agtcaaaatt gttacactgt ctgaccaacg catattggat gaactgagca
     1801 tcgccaaaat cggcaagcat ctcaacgatc tggtgctgca gaatccgaaa atcaaaataa
     1861 tacttgattt cgtaaacgtt accaacatgt cgtccagcgc actgggcatg ctgataaccc
     1921 tgcataaacg tgttcgtgaa gccggcggag aacttcgcct gtgcaatatt cagcccacaa
     1981 tccaggaagt gttcaaaatc accagactcg acgaaatctt ccagatatgc gtgagtaagg
     2041 gtgaagcaat cgatagcgtt gaatgatggc tgaagcgtgg cagttcatct gcgggaaatc
     2101 tgtcgttacc ggaatgtgaa ctttggtaaa aaaacaaatt atcaaatctg attacgaccg
     2161 tgcaagagtt gtggaagagt ctattatttc cgaggcacag gcaaacgggt tcagtgatgt
     2221 cgacgtattt gccattcgcc ttgccctcga agaggctctg gtcaacgcca tccgtcatgg
     2281 caacaggctt gacccgcata agaaagtgac tgtcgagtgg tgcgtccgta acaaagaact
     2341 ggagattacg atagaagatg agggcaaagg ttttttgccg aatgccgtgc cggatccgac
     2401 gcaggatgaa aatctcgaaa ggcccggcgg caggggactg atgctgatga aggcttacat
     2461 ggattgcgtg gaatataaca aacagggcaa tatcgtaaga atgtgcaaaa agttacattg
     2521 ctgcgggaaa tgaaaagcga atttttgcgg ggcagatggc tgtgatgaga gtgggcggat
     2581 gatgattttc aatgattcaa acaaattgaa accgcattgg tttttttgtt gcatattgtg
     2641 aggtagaaaa ttatgaaggc ggtagcgttt aacgggagtc cgagagtcgg cggcaatacg
     2701 gagtttttac tcaaaaccgt gctggaacag atttctgcag ccggccatga aactgaattt
     2761 gtgcaggtag gcggaaaact tctgcatggc tgtaccgcgt gtcttaagtg tcgcgagaat
     2821 aaaaatcgtc tttgcgtcat aaccaacgat atcgtcaacg actgcattgc caaaatgata
     2881 gaggctgacg cgattcttct gggttcgcct gcatattttg cggacatgac gcctgaaatg
     2941 aaagctctca tcgaccgcgc cggctacgtt tgcagcggca acggccgcct gttgaagcat
     3001 aaaataggtg ctgccgtagt ggctgcccgt cgcggcggag ctacgcatgt gctggactca
     3061 atcaaccatt tcttcctggt atcgcagatg attgtccctg ggtcgactta ctggaatttc
     3121 ggcataggca gggaaaaagg tcaggcggct aacgattcgg aaggagtggc taatatgcag
     3181 gatctcggcc agacaatcgc atggctgatg agcagactgt aaaaattgta gcccatccct
     3241 cattggccta aaatcagcca tttttcagcc tggtctgcgt cataaaacgg tttgcttcct
     3301 tacggcagaa agttgccggc tcgaagtttt tccggcagat aggtattggc gacaaactcc
     3361 agcccgtgtt ttgccaacgc ctgttgctcg acacgaacgc ctagtttcag catatgattg
     3421 attgccttct gccatacgac gtaatgctgt atgaacggat catttttcat ggcgtcctgt
     3481 gcgcgttttt tgtccacgtc ttttaacggg tgcgtcggca ggtcataggc tttgatgtcg
     3541 tccgg
//
LOCUS       DAONFI010000249         3542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_32404_length_3542_cov_3.868368, whole genome shotgun sequence.
ACCESSION   DAONFI010000249 DAONFI010000000
VERSION     DAONFI010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32404_length_3542_cov_3.868368"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PLK08_08030"
     CDS             <1..1326
                     /locus_tag="PLK08_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HPS53486.1"
                     /translation="ATFGPITKKVSVDDVATLNNIRKYCTGVKISDPDASGKVTVTFG
                     HVVIDLDKLEPMAAGSPSPDNIKTVRELAGQPVRQVVIGSCTNSSYQDLMLVAGVLKG
                     RTVHPDVELGIAPGSRQVMTMLGRNGALADLILAGARILESGCGPCIGQGFSPADGTV
                     SLRTFNRNFAGRTGTKGDMVWLVSPETAIAAAITGKITDPRDLNIDIKYPGVEMPQKF
                     LIDDNMLQKPLSPEDAARVEVLRGPTIVKPPAGEVLPNKLEAKVAIKVGDKITTDHIM
                     PAGSLLKYRSNVPQYARYVFNCFNEDGQPTFADRCLELRQKGVGAVIVAGDSYGQGSS
                     REHAALCPMYLGVRVVIAKAIERIHQANLVNFAILPLVFVDPADYGRIDAGDGIVLEN
                     LEQAVTSADVVKVRNATKGFDFNCRVQLAPRQRKILAAGGLLNYTRKNG"
     gene            1345..1896
                     /locus_tag="PLK08_08035"
     CDS             1345..1896
                     /locus_tag="PLK08_08035"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HPS53487.1"
                     /translation="MTAKIISVFGANDGQSGDEAYEQARSVGKFLAERGYAIANGGYG
                     GTMQAAAAGAKSAGGTTYGVVCDIWQTPPNDYIDHVVRTSTYMERLQTLIDFGKSGYV
                     VLPGATGTLMEAAMVWEHACKRFWEKADQPSRPIAFIGDFWLPLRSMMTQARPGCEKY
                     VAFLSTADELTEIFPAVKVTRMD"
     gene            1977..3260
                     /locus_tag="PLK08_08040"
     CDS             1977..3260
                     /locus_tag="PLK08_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53488.1"
                     /translation="MAAAEDGAVVPLSELKKSVILKKTPAAEQTGQPSTAASSSNDDE
                     SKPSPAIPELPSSSGGEIPAVPEMPSDNELQTLPSGPAASTTTKVMPKSTPQIETEGF
                     FDDESSLPAPMGPTTASKMDMRDMFGKKTIPVTMSRDRRIIHSDMHQLNALPENASVI
                     AQPGKIYKGSDGWYWVRYEASHGKTPRAQQRLLPCRILQSVEEQLAVKPDTLFDIYGQ
                     AMLYDGMTYLLLRRVTIEDGNSQRIEDASVKNVQDEEPDLLRPTGKSLQQTTVASVNT
                     ATTVEKSSSAAEAISKTDDLLAGMMSETEGRAIRTTNAPSRSAEENKTSVAPAGQMPL
                     TQGRENLVIDRLARVVKSKHSDWYEVHFISDNTLREPPYLVLPNSRLTESLRLNSYLG
                     YHDRKLRISGEVIFYKGRRYILLTKVFPERDMRQF"
     gene            complement(3330..>3542)
                     /locus_tag="PLK08_08045"
     CDS             complement(3330..>3542)
                     /locus_tag="PLK08_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53489.1"
                     /translation="AGKLAASFKRKIILRTALATSIFKFVARGRSQLPCERKKVRDVE
                     KGFRCSLTQPADAANSNKRKVKFNML"
BASE COUNT          853 a          922 c          984 g          783 t
ORIGIN      
        1 gcaacttttg ggccgattac taaaaaagtt tcagtcgacg atgtcgccac actgaacaat
       61 atacgcaaat attgtacggg cgttaaaata tccgaccccg acgcgagcgg caaggttacc
      121 gtcacatttg gccatgtggt gatagacctt gataaactcg aaccgatggc tgcgggttcg
      181 ccgagtcctg acaatatcaa aacggtgcgg gagctggccg gccagcctgt ccggcaggtg
      241 gttatcggtt cgtgcacgaa cagcagctat caggatttga tgctcgtggc aggcgtgctc
      301 aaaggccgaa cggttcatcc ggatgtggaa ctcggtattg cgcctggcag caggcaggtg
      361 atgacaatgc tgggcaggaa cggcgcactt gctgacctca tattggctgg cgcgcgaatt
      421 ctggaaagcg gctgcgggcc ttgcatcggg caggggttca gcccggccga cggcacggtc
      481 agcctgcgga ctttcaatcg caacttcgcc ggcagaacag gcacaaaggg cgatatggtc
      541 tggctggtgt ctccggagac cgccatcgcc gccgcaatta cgggaaaaat aaccgacccg
      601 cgggatttga acatcgatat aaaatatccg ggagtcgaaa tgccccaaaa atttctcatc
      661 gatgacaata tgttgcaaaa accgctttcg ccagaagatg ctgccagggt ggaagtcctg
      721 cgtgggccga caatcgtcaa gccgccggct ggcgaagttc tgccgaataa acttgaagcg
      781 aaagtcgcca tcaaggtcgg cgacaagata accaccgacc atatcatgcc cgccggctca
      841 cttttgaaat atcgctccaa tgtgccgcaa tacgccagat atgtcttcaa ctgcttcaat
      901 gaagacggcc aaccgacttt tgccgaccgc tgtcttgaac tcaggcaaaa aggcgtcggt
      961 gcggtaatcg tcgcaggcga cagctacggc cagggttcaa gcagggaaca tgccgctctc
     1021 tgcccgatgt atctgggcgt gcgcgttgtc atcgcgaagg ctatcgagcg gattcatcag
     1081 gctaatctgg tcaacttcgc gattctgccg ctggtgttcg tcgacccggc cgattacggc
     1141 cgaatcgatg ccggcgacgg aattgttttg gaaaatctgg agcaggctgt cacttcagcc
     1201 gacgttgtta aagtccgcaa tgcgacgaaa ggttttgatt ttaattgcag agtccagctc
     1261 gcgcctcgtc agcgaaaaat tcttgcggcg ggcggcttgc tgaattacac gcggaagaac
     1321 ggctgatttt tttgagcgaa atagatgact gcaaaaatca taagcgtatt cggagctaac
     1381 gacggccagt ctggcgacga ggcctatgag caggctcgta gcgtcggaaa atttctggct
     1441 gagcgcggtt acgccatcgc gaacggcggt tacggcggca ctatgcaagc cgctgccgcc
     1501 ggtgctaaat cagccggcgg aacaacctac ggcgtcgttt gcgatatctg gcagaccccg
     1561 ccgaacgact atatcgacca tgtcgtgcgg acgtcgacgt acatggagag gctgcagacg
     1621 ctgattgatt ttggcaaaag cggctatgtt gtactgccag gcgcaaccgg cacgctgatg
     1681 gaagcagcca tggtttggga acatgcctgc aaacgttttt gggaaaaggc tgaccagccg
     1741 agccggccga tagcgtttat aggtgatttc tggctgccgc tgcgaagcat gatgactcag
     1801 gctcggccgg gctgcgaaaa atacgtcgcc ttcctgtcaa cggccgatga attgacggaa
     1861 atctttccag ccgtaaaagt tacacgtatg gattgaacta ccctcgctgg aaaagtataa
     1921 taaaccgaat gaggtcacga cccttaatat tattgatatt gttttcgacg gcgtgtatgg
     1981 ctgcggctga agacggcgcg gttgtacctc tgtcggaact taaaaagagt gtgattctca
     2041 aaaaaactcc ggcggctgag cagacaggcc agccgtcgac ggctgcgtct tcatcgaatg
     2101 acgacgagtc caagccttcg ccggctattc ccgaattgcc gtcgtcgtcc ggcggcgaaa
     2161 ttccggctgt gccggaaatg ccttccgata acgagcttca gacactgccg tccgggccag
     2221 ccgcttcaac tacgacgaaa gtgatgccca aatcaacgcc gcagattgag acggaaggtt
     2281 tttttgacga tgaaagctct ctgcccgcgc cgatggggcc tacgacggca tcgaagatgg
     2341 acatgcgtga catgttcggc aaaaagacca ttcccgtcac aatgtcgcga gaccggcgta
     2401 taattcatag cgacatgcat cagctcaacg cactgccgga aaacgcttcc gtaatcgcac
     2461 agccggggaa aatatacaaa ggttccgacg gctggtactg ggtcaggtat gaagcgtctc
     2521 acggcaaaac gccgagggct caacagcgtc ttctgccgtg ccgcattctc cagagtgtgg
     2581 aagaacagct tgccgtcaag cccgacacgc tttttgacat ttacggccag gcgatgcttt
     2641 acgacggcat gacgtatctg ctgttgcgac gtgtgaccat agaagacgga aacagccagc
     2701 gaatcgaaga tgcgtccgtg aaaaatgtac aggatgaaga gcccgacctt ttacggccga
     2761 ctggcaaatc gctccagcag accacagttg catctgtaaa taccgcgacg acggttgaaa
     2821 agtcgtcttc tgcagctgag gctatctcga aaaccgacga cctgttagcc ggcatgatga
     2881 gcgaaactga aggcagggct atacgcacga ccaatgcgcc gtcccgttcg gctgaagaga
     2941 acaaaacatc agttgcgccg gctggccaga tgccgctgac gcagggccgt gaaaatctcg
     3001 tcattgaccg actggcacgc gtggtgaaat caaaacactc agactggtat gaagtgcatt
     3061 ttatcagcga taacacgctt cgtgagccgc cgtatctggt tctgccgaat tcgcggctga
     3121 cggagtcgct gaggttgaat tcgtatctcg gttatcacga ccgcaagctc agaatctccg
     3181 gcgaggtgat tttctacaag ggccggcgtt acatcctgct gacaaaggtt ttccccgaac
     3241 gcgatatgcg tcaattctga tttttcaaca acggcagatt cgacttgcag aaaccctgtt
     3301 tgggcttcat gtcggtatta tcgcggttct tagagcatat taaacttgac tttgcgtttg
     3361 ttgctgtttg cggcatcggc tggctgcgtc agactgcatc ggaaaccttt ttcaacatct
     3421 cttacttttt tgcgttcgca gggtaattga gaacgccctc tagctacaaa cttaaatatt
     3481 gaagttgcta gagcggttct caaaattatt ttccttttga agcttgctgc aagtttccct
     3541 gc
//
LOCUS       DAONFI010000250         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_32746_length_3516_cov_4.905230, whole genome shotgun sequence.
ACCESSION   DAONFI010000250 DAONFI010000000
VERSION     DAONFI010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32746_length_3516_cov_4.905230"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="PLK08_08050"
     CDS             complement(<1..1790)
                     /locus_tag="PLK08_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53490.1"
                     /translation="MKTIREHLVRRFPQQRRRRWKYVSPIRRRVGLGILAFLLVTIYL
                     GWYFTRDSNVRREAKKALEDLTGADVHIDRAHFSLFEGIRLYGVRVTLPDERSPYPFF
                     TAGEVVIKHRPMELIRHGRLKINEIVCMQPVVTVEYDKAGGMSNAERLFRLAVEHNRK
                     KPADRYQPMQFPRIFLRDGYLKSLVKSGDTTQRTFEEGMSCELKPIGNNLVMVTVESE
                     HENGEKIQWARIELDTSDGRIAGMSGSASHRLFQLLPPQYHKWIQQYDPQGDFSVVEG
                     EGTDPQDGRFEIRLDDFSFNMPDGDKVFAVKNVTGKLLFRRDGIEFKSVTGRPVLLGP
                     DARIRIDGHCDGYSSDSSFDLTVYVKKILLPQTSLPGRLAVICRVVNRDYQPRGLANM
                     GLRIVRDKNGDLQVAGIIKPEKLRTCCRFFPIEAQAAGGSITFTQKSIEDINLDLYRG
                     QSRFTVRGSARHIEGMNWDYDVSIDGSDVKLDDSLRDALPNQARKIYDSLSPAGKCNG
                     KVIAWKKGEDRGIKVDLRMNGFASVCYDGFPYRLENLSGEVHVENGNISVPWAQSEKG
                     DMVVRMNGRFSDGCADMDIAAKNLPLDKKL"
     gene            complement(1774..2937)
                     /locus_tag="PLK08_08055"
     CDS             complement(1774..2937)
                     /locus_tag="PLK08_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53491.1"
                     /translation="MADLESIFDASVEICPPVKDGDLAAIPAKRGVVMLSAAGGQPIL
                     VLTAADMRSRVQTRLGEPADDARGKKIDLREITRKVHYSRCASHFETDLRYLRTVVKI
                     FPHRYEQMVAWQAPWFVSLDVKSRVPRFSRRRGISASDATERYFGPFAAGRDADRFID
                     SLADAFRLCRDVNCLRDAPNHGGCTYAEMNRCVGVGVGRMSMEDYRLLLETAGDFAAG
                     RRDGLREKITADMQAHVKALEFEQAGVCKRRLERLSDFDLPAYRYVRDVKDLEFLLVH
                     RGMSAGEYRFFSVVPGEVAEPFVLEKPATPEKVQKILAYHDEHRRNASPCCGVEADLT
                     MGLVTRYLFCEPAKAGLVVPGPITQDAASVITEFVNRSLKGDGPQAEQDENNS"
     gene            complement(2956..3426)
                     /locus_tag="PLK08_08060"
     CDS             complement(2956..3426)
                     /locus_tag="PLK08_08060"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HPS53492.1"
                     /translation="MPATPFMELDKLDLNHIVADHDKIYSILPHRYEFERLTAIVHMD
                     DMSVAGYHDVGHDEFWVKGHIPGRPLFPGVMMIETAAQLVSYHALVHTSGRSGFLGFA
                     GVDGVKFRDSVLPGQRLVMLAKMVEFRPRRCTADTQGFVDGKMVFEGRITGMWL"
BASE COUNT          767 a         1064 c          851 g          834 t
ORIGIN      
        1 aaaagttttt tatccaacgg cagatttttg gcggcaatgt ccatatcggc acagccgtcg
       61 gagaatctgc cgttcatgcg gacgaccatg tcgccctttt cactctgagc ccacggcacg
      121 gaaatattgc cgttttccac gtgcacctcg ccggaaagat tttccagccg gtaaggaaaa
      181 ccgtcataac ataccgacgc aaagccgttc atccgcaaat cgacttttat acccctgtcc
      241 tcaccctttt tccacgcaat caccttgccg ttgcacttgc ccgccggcga aagtgaatcg
      301 tatattttcc gagcctgatt cggcagtgcg tcgcgaagcg agtcgtcaag ttttacgtcg
      361 ctgccgtcta tacttacgtc gtaatcccag ttcattcctt ctatatgcct ggcactgccg
      421 cgcacagtaa acctggattg gccgcgatac aaatcaagat tgatatcctc tatgctcttt
      481 tgcgtaaacg ttatgcttcc gccggcggcc tgcgcttcta tcgggaaaaa acggcagcat
      541 gtcctgagtt tttcaggttt gattatgcct gctacctgga gatcgccgtt tttatcccgt
      601 actatccgca agcccatgtt tgcaaggccg cgcggctgat aatcgcgatt caccactctg
      661 caaatgacgg cgagtcgccc cggcagagag gtctgcggca aaaggatttt cttcacgtac
      721 accgtcaggt cgaacgaact gtcggaagaa tatccgtcgc aatggccatc gattctgatg
      781 cgcgcatcag ggccgagcag cacaggccgg ccggttacac tcttgaactc tatgccatcc
      841 ctgcggaaca gcagtttgcc ggtaacgttt tttacggcaa acaccttgtc gccgtcgggc
      901 atattgaacg aaaaatcgtc cagcctgatt tcaaaacggc catcctgcgg gtccgttcct
      961 tcgccctcga ctacgctgaa gtcaccctgc gggtcgtact gctgtatcca cttgtgatat
     1021 tgcggcggca acagctggaa aagcctgtga ctcgccgagc cggacatgcc ggcaatccgg
     1081 ccgtcggacg tatccagctc tatcctggcc cattgtattt tttcgccgtt ctcatgctcg
     1141 ctttcaacgg tgaccatgac gaggttatta ccaatcggtt tcaattcgca gctcatgccc
     1201 tcttcaaacg tccgctgagt cgtatcgccc gattttacga gagatttgag atagccgtct
     1261 cgcaggaata ttcgtgggaa ttgcatcggc tgatagcggt cagcgggctt tttgcggtta
     1321 tgttcgacgg cgagccggaa tagtcgttcg gcgttgctca tgccgccggc tttgtcgtat
     1381 tccacagtaa ctaccggctg catacagact atttcgttta tcttgagtcg gccatgccgg
     1441 ataagttcca tcggccggtg ttttatgacc acttcgccag cggtgaagaa cggatacggc
     1501 gagcgttcgt ccggcagcgt gacccgcacg ccgtaaaggc gtatgccctc aaaaaggctg
     1561 aaatgcgccc tgtctatatg cacgtcggca ccggtcaaat cttccagtgc tttcttggcc
     1621 tcgcggcgaa cgttggaatc acgggtaaaa taccagccca ggtatatcgt caccagcaga
     1681 aacgccaata tcccaaggcc cactcgcctg cgaatcggcg aaacgtattt ccaccgccgc
     1741 cttcgctgct gcggaaaccg cctgacaaga tgttcacgaa ttgttttcat cctgctctgc
     1801 ctgagggccg tcgcccttca atgaccgatt cacaaattca gtaatcaccg acgcggcatc
     1861 ctgcgtaatg gggccaggca ctacgaggcc tgccttcgcc ggttcgcaga aaagataccg
     1921 cgtaacaagg cccatcgtca aatccgcctc aacgccacag cacggcgacg cattacgacg
     1981 atgttcgtcg tgatatgcca gaattttctg cactttttcc ggcgtcgcgg gtttttcgag
     2041 aacgaaaggc tcggccacct cgccagggac aacagaaaaa aatctgtatt cgcctgcact
     2101 catgccgcga tgcacaagca gaaattccaa atcctttacg tcccgtacat atctgtacgc
     2161 cggcaaatcg aaatcactca gcctctcaag tcgcctcttg cagacgccag cctgttcgaa
     2221 ctccaacgcc tttacatgcg cctgcatgtc ggcggttatt ttttcacgca aaccgtcccg
     2281 ccgtccggcg gcaaaatcgc cggctgtttc aagcagcaag cgataatctt ccatgctcat
     2341 gcggccaact cccacaccca cgcagcggtt catctccgca taagtacagc cgccgtgatt
     2401 cggcgcatcg cgcagacagt tcacatctct gcacagccga aacgcgtccg ccagcgaatc
     2461 gataaatctg tcggcatctc tgccggcggc aaaaggccca aaatatcttt ctgtcgcgtc
     2521 ggaggcagaa attccgcggc gacggctgaa ccgcggtaca cggcttttca cgtccagcga
     2581 aacaaaccac ggcgcctgcc atgcgaccat ctgctcatac ctgtggggaa aaatcttcac
     2641 gaccgtccgc aggtatcgca aatccgtttc aaaatggctg gcacaacggc tgtaatgaac
     2701 ttttcgcgtt atttcgcgca ggtcgatttt tttgcccctg gcgtcgtccg ccggctcgcc
     2761 gaggcgggtc tgcaccctgc ttcgcatatc agccgccgtc aggacaagta tcggctggcc
     2821 gccagccgcc gacaacatca ctacgcctcg tttggcaggt attgccgcga ggtcgccatc
     2881 cttcaccggc ggacaaatct ccacacttgc gtcgaatatg ctttcaaggt cagccatcgg
     2941 gtattttttt ctgctctaca gccacatgcc ggtaattctg ccttcgaata ccattttccc
     3001 gtcgacgaaa ccctgggtgt cggcggtgca acgtctcggc cgaaattcca ccatcttagc
     3061 cagcatgacc aaccgctgcc cgggcagaac actgtcgcgg aattttacgc cgtcaacgcc
     3121 ggcaaagccc aaaaagccgc tcctgccact cgtatgaacc agggcatgat agctcacaag
     3181 ctgggctgcg gtctctatca tcattacgcc cggaaaaagc ggccggccgg gtatatggcc
     3241 cttgacccag aattcatcat ggcccacgtc atgatagccg gctacggaca tgtcgtccat
     3301 atgcactatc gcagtgagtc tctcgaattc gtagcggtgc ggcagaatgc tgtaaatctt
     3361 gtcgtggtcg gcgactatgt gattcaggtc tagtttgtcc aattccatga atggggttgc
     3421 gggcataatt catttccttc ctgtcattca aacaacttac taattagaac ctatcccaaa
     3481 aaccttggct gcctgcaatc ggctcttttg cagcct
//
LOCUS       DAONFI010000251         3498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_32996_length_3498_cov_3.348243, whole genome shotgun sequence.
ACCESSION   DAONFI010000251 DAONFI010000000
VERSION     DAONFI010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32996_length_3498_cov_3.348243"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..2753
                     /locus_tag="PLK08_08065"
     CDS             138..2753
                     /locus_tag="PLK08_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53493.1"
                     /translation="MKSKDTIKNGYGRLETLLLILIAVVLAGFLAGSRAMAQDKDAAG
                     SESKSIFDDSDSGDAKSSADSKSGQAEDSFEEDEADDAKSKKKDDGILNEVRMSTDGT
                     FELHIKNADLLDVLKHLSEQGRINIIVSKNITGKVTLDLFGVSLMEAIEAVLRSNNLC
                     YRKEGNFVYVYTEKEYEEARKLAFKVFRLSYIQAEDAQKLVSAVLSDKGKITTTPPIR
                     KGIVDVATDQADQGGGMSHAADEIIVVHDYEDNIKKVEEILKSIDILPQQVLLEATIL
                     SAELDEDTSLGVNIQALSGATMGAFNATTDLTGVTFEDAAVSGLSGNTQGRFNLPFDA
                     QVADGATPMSVGFIYNNTAVFIQMLEQVTNTSIIANPKMLVVNKQRGDILIGTEEGYL
                     TTEVSETFTSQKVEFLKTGTILQVRPFIGKDGFIRLEIHPEVSEGSVRILSNASEDSP
                     ALPTKKTTTITTNVIVRDGHTVVIGGLFRERIKTQRSQVPVLGDVPYVGTAFRSTYDT
                     TVREEIIILITPHLIRHTKYEQFSEQIKDEVERFRVGTRKSIQWFTRSRIADTYINHA
                     KQLIRENRKGTALWYLDLALSIEPQEESAVKLREEITGSPYWSSVPRFSATQQFIERA
                     IMCDLGISPNSVQYPTKPLDVNLIPQKARSRLGIDPSLDKRLDALYVEKTYQGPSSDA
                     VPQPMSDTQSVPPTTPVKKTSPAAGQRQTTSPINTSHVPTKAVSPATRTTVAAQPVVV
                     TPRQTVRTVKAPLPARPVAAEVTSRSTVKTTKISDVSSHKSSYAGDVTARPAASKMES
                     LMPSRRVKLETAPLMADPKPVSLQPEVKKLSTRPISDPKPQSLDTAKTPENGESKNWS
                     VWVLWDEDTPLSKAE"
     gene            2817..>3498
                     /locus_tag="PLK08_08070"
     CDS             2817..>3498
                     /locus_tag="PLK08_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53494.1"
                     /translation="MKKIIAYLMIISFCISLFGGCNMSNKDKDLNTAKKEAYQSWAKS
                     RIELYLQLANDNYKSGRLDEAKEKIREVLKLDSENLTAHLFLAKINIEQGLFDVASKE
                     LEYVIAKNSQSYEAFYMLGVALEYKDKLDQSLANYEQAAQLNPAEMAPILAAAEVMVK
                     QDRAGEALMLLERKMNMSADKPQTYELAGRISMMLGKYDRAVDYFQKACDVDFKNKRY
                     PEMLAQAQK"
BASE COUNT          963 a          855 c          943 g          737 t
ORIGIN      
        1 ggcggatgga tgtcgcttaa aaaatacttt ttgtaaaaaa atccaaaaaa gcccgaacaa
       61 aaaccccctg aatatccgat atcttttgag tagtagtatc tttgcgcaga gaaactatga
      121 ttgtatggag tggttaagtg aagagtaagg ataccataaa aaacggttac ggcaggcttg
      181 aaacactgtt gctgatattg attgcagtgg tgttggctgg ctttttagcc ggcagtcggg
      241 ccatggctca ggacaaggac gccgcaggct cggagtcgaa aagtattttt gacgatagtg
      301 attctggtga tgccaaaagt tccgccgact cgaaaagcgg gcaggctgag gactctttcg
      361 aagaagacga ggctgacgat gccaagtcga agaaaaaaga tgacggaatt ttaaacgaag
      421 tacgcatgag tactgacggc actttcgaac tgcatatcaa aaatgccgac ctgctggacg
      481 tactgaagca tctttccgag cagggccgta taaatatcat cgtttccaag aacataaccg
      541 gcaaggttac gctggatctt tttggcgtat cgctcatgga agccatcgaa gccgttcttc
      601 gttccaacaa cctctgctat cgcaaagagg gaaatttcgt ctacgtctat accgaaaagg
      661 aatatgaaga ggcacgcaag ctggcgttca aggtttttcg tctgtcgtat attcaggcgg
      721 aagatgcgca aaaacttgta tccgccgttt tgagcgacaa gggcaagata acgacgacac
      781 cgccgatacg caaaggcata gtcgatgtcg ccaccgacca ggcagaccag ggcggcggca
      841 tgagccatgc ggctgatgaa attatcgtcg tccacgatta cgaggacaat attaaaaagg
      901 ttgaggaaat cctcaagagt atagatattc tgccgcagca ggtactgctt gaggcgacga
      961 tactttccgc cgaactggat gaagatacct cgttgggcgt gaacattcag gcgctcagcg
     1021 gtgcgacgat gggtgcgttc aacgccacta cggacctgac aggcgtaaca tttgaagacg
     1081 ctgccgtttc agggcttagc ggtaatacgc aaggccgctt caatctgccg ttcgacgcgc
     1141 aggttgcgga cggcgctacg ccgatgagtg ttggattcat ctacaacaac actgccgttt
     1201 tcatccagat gcttgaacag gtgacgaata ccagcattat cgccaatcct aaaatgctgg
     1261 tcgtcaacaa gcagcgcggc gacatcctta taggcactga agagggctat cttaccacgg
     1321 aagtgtcgga gacgttcacg tcgcagaagg tggagttcct gaagacaggt acgatacttc
     1381 aggtcaggcc ttttatcggc aaggacgggt tcatcaggct tgaaattcat ccggaagtgt
     1441 ctgaaggcag cgtgcggatt ctgtcgaacg cgtctgaaga ttcgccggct ttgccgacca
     1501 agaagaccac gaccataacc accaacgtca tagtgcggga cggccatacg gtggttatcg
     1561 gcggcctttt cagggaacgc atcaagaccc agcgttccca ggtgcctgtc ctgggtgacg
     1621 tgccgtacgt aggcactgcc ttccgcagta cgtatgatac gaccgttcgc gaagaaatca
     1681 ttattctgat aacgccgcat ctgattcggc acaccaagta cgagcagttc agcgagcaga
     1741 taaaagacga agttgagcgt ttccgtgtcg gcacacgaaa gagcatacag tggtttaccc
     1801 gcagccgcat tgccgacacc tatataaatc acgcgaaaca gcttatacgt gaaaatcgca
     1861 agggcacagc cttgtggtat ctcgaccttg cactctcaat agagccgcag gaagaatccg
     1921 ccgtcaagct gagggaagag ataaccggct cgccgtattg gtcgagcgtg ccgaggttct
     1981 cggctaccca gcagttcata gagcgggcga taatgtgcga tttgggcata agccccaaca
     2041 gcgtgcaata ccccacaaaa ccgctggacg tgaatctgat accgcagaag gcaagaagtc
     2101 gcctgggcat agatccttca ctggataaac ggcttgatgc gctgtatgtt gaaaagacgt
     2161 atcaggggcc gtcttccgat gctgtgcccc agccgatgtc agacactcag agcgttccgc
     2221 cgacaacgcc ggtgaaaaag acctcgccgg cggccggaca gagacagacg acttcgccga
     2281 taaatacgtc gcacgtgccg acaaaagcag tttctccggc aacacggaca acggtcgcag
     2341 cacagcctgt tgttgtaacc ccgcggcaga ctgtccgcac ggttaaagca ccgctgccag
     2401 ctcggcctgt tgctgccgag gtaacttcaa gaagtactgt gaagacgacg aaaatatccg
     2461 acgtttcatc gcataagtcg tcatatgccg gcgatgtgac ggcaaggccg gctgcgtcga
     2521 agatggaatc gctaatgccc agccgtcggg ttaagttgga aactgcgccg ttgatggctg
     2581 accccaagcc ggtttccctt cagccggaag tcaaaaaact ttccactcgt ccgataagtg
     2641 accccaaacc gcagtcgctg gatacggcaa aaactcctga aaacggcgaa tcgaaaaact
     2701 ggagcgtatg ggtgttgtgg gatgaggata cgccgctttc caaagcggaa tgacgacggc
     2761 aggcttgatg cccgttttga ttcttgcgat ttctggaatc ttatgaggat gatgatatga
     2821 aaaaaatcat tgcatatctg atgataattt cgttttgcat cagtctgttc ggcggctgca
     2881 atatgtccaa caaggacaag gatctgaaca cagccaaaaa agaggcgtat cagagctggg
     2941 ccaagtccag gatagaactt tatctgcaac tggctaacga caattacaaa tccggccggc
     3001 tggacgaagc taaggaaaaa atcagggaag tcctgaaact cgatagcgaa aatttgacgg
     3061 cccatctgtt tctggcgaaa atcaatatag aacaggggct ttttgatgtg gcgagcaagg
     3121 aactggaata tgtcatcgcc aaaaattccc agtcatacga agcgttctac atgttgggcg
     3181 tagccctgga atataaggac aagctcgatc agtcgctggc caactatgaa caggcggcac
     3241 agctgaatcc ggcggagatg gctccgatat tagccgccgc cgaagtcatg gtcaaacagg
     3301 acagagccgg cgaagcactg atgctcctgg aacgaaagat gaatatgtcc gccgacaagc
     3361 cgcagaccta cgaacttgcg ggcaggattt caatgatgct cggcaaatat gaccgggccg
     3421 tcgattactt ccagaaagcc tgcgacgtgg atttcaaaaa caagcgatat cctgaaatgc
     3481 tcgcccaggc acagaaag
//
LOCUS       DAONFI010000252         3493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_33057_length_3493_cov_4.362420, whole genome shotgun sequence.
ACCESSION   DAONFI010000252 DAONFI010000000
VERSION     DAONFI010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33057_length_3493_cov_4.362420"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLK08_08075"
     CDS             <1..1439
                     /locus_tag="PLK08_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53495.1"
                     /translation="RPHVDFISPMGVFQYMLVHLGILICGVKGAALTMGYLAFMPMVL
                     ISTWVICSKRMAYLPSSFITIAAVSLMLGANALGTPLPASSYETVYNRLGLAMISPLL
                     VAALLPKIRCGDFVDGICIGLMLGCAFFLKINFGIGGILLLLLGVFAKKIPISKLTLI
                     FAGFLTVTVIVAVYLHGRLDAFFFDMFRLIKNSGSNVASICVTKAWFNLLDVNFGLMI
                     LSVCLWGGIAAGNGSSISGDARKPRFFVNFTKNLTILIIAAAIVFFVMVGCMKLDKFM
                     FPYIFIFAVFGWTDAIFRNQPTWRGFVIGMLGFVCAFFFAFGQTQSTVNALRHRLLIN
                     NSARRYCLLTNPALNDMPFILDGMPVAENLNLGTELLAKNRVPKNYVVYSLTMFNNWP
                     LLYGSAYRKGDWAWLHYGLLFDGSHQPSRHELFDDVDAVLVSKKDNYANYSKEILSRY
                     GQYFYDNYYLKDENSDWRLYLKKSSAVK"
     gene            1585..2625
                     /locus_tag="PLK08_08080"
     CDS             1585..2625
                     /locus_tag="PLK08_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS53496.1"
                     /translation="MMARYLITGGAGFLGINMARFLLDKAQDVTSLDVAPFDYLERDR
                     VREITGDIRDAAKLDEALQDVHVVIHTAAALPLYKKSEIFSIDIDGTRNVMERAYAAG
                     VKRVVHVSSTAVYGVPDHHPIFENDRLVGVGPYGVAKISAEKICAEYRDKGMCVSIVR
                     PKSFIGPERLGVFALLYDWAKDGRNFPMIGSGNNRYQLLDVEDLCRAIWLCATLDEKI
                     CNDVFNIGAKDFTTMKEDFQAVLDDAGFGKHIKGFPAWPMIWTLRILEFFRLSPLYKW
                     VYETACTDSFVSVEKAEKVLGFTPEFSNKDALLRNYRWYLQNLASFQGRSGISHRVPW
                     KQGILALAKKMF"
     gene            complement(2643..>3493)
                     /locus_tag="PLK08_08085"
     CDS             complement(2643..>3493)
                     /locus_tag="PLK08_08085"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPS53497.1"
                     /translation="WISEGRVLPNDLVWAEGMENWVKAYVIMNGTTAEIKGTGGMTPC
                     KDIRSKARTALAGKWGMCALYMLGFFVITSLISAFTTYPPFFARHEYREAEVNWGQLL
                     LNGPFTLGFAMFFLGVGRGGMPSFGTFFAGFKRFIGAFCLYWCMLVFVLLWMLLLIIP
                     GIMAAYSYAMAYYVYADNPELGALGAIKRSKELMYGHRLKLFWLGLTFIGWGFLCILT
                     FGIGFLWLVPYFNTALAVFYHDIQQPALADISESAPQGQPAESTVQTENNEPPMDLSR
                     PINPQQ"
BASE COUNT          850 a          817 c          896 g          930 t
ORIGIN      
        1 agcggccgca cgttgatttc atatcgccga tgggcgtgtt tcagtatatg cttgtgcatc
       61 tggggattct gatttgcggc gtaaaagggg cggctctcac gatgggatat ctggcgttca
      121 tgccgatggt gttgatatcc acatgggtga tttgctcaaa gcggatggcg tatctgccgt
      181 cgagttttat aaccatagct gccgtttctc tcatgttggg tgcaaatgcg ctcggcacgc
      241 cgttgccggc gtcgagttat gaaacggtct ataaccggct tgggctggcg atgatttcgc
      301 cgctgttggt ggcggcgttg ctgccgaaga tacgttgcgg tgattttgtc gacggtatct
      361 gcataggcct gatgctcggc tgtgcgtttt tccttaaaat aaatttcggc atcggcggaa
      421 ttcttctgct tttgctcggt gtgtttgcta aaaaaattcc gatttcaaag ctgacgctga
      481 tttttgcggg ttttctgact gtgacggtta tcgtagccgt gtatctgcac ggccgactgg
      541 acgccttttt tttcgacatg ttcaggctga taaaaaattc cggcagcaac gtagcgtcca
      601 tctgcgtgac gaaagcctgg ttcaatcttc ttgacgtgaa tttcggattg atgatattgt
      661 ccgtatgcct atggggagga atagcagccg gaaacggctc atccatttcc ggcgacgcac
      721 gaaaaccgcg atttttcgta aattttacga aaaatctcac gatcctcatc attgccgccg
      781 caatcgtctt ttttgtgatg gtagggtgca tgaaattgga taaattcatg tttccttaca
      841 ttttcatttt tgccgtcttc gggtggacgg atgcgatttt tcgcaatcag ccgacgtggc
      901 gaggctttgt cataggcatg ctgggcttcg tgtgcgcgtt ttttttcgca ttcggccaga
      961 cgcagtcgac agtcaatgcc ttgcgtcata gattgttaat aaacaattcc gcccgcagat
     1021 attgtctgct gacgaatccg gcattgaatg atatgccgtt cattctcgac ggcatgcctg
     1081 tcgcggaaaa tctcaacttg ggtacggaac tgcttgccaa aaaccgcgtg cccaaaaact
     1141 acgtcgttta ctcattgacc atgttcaaca actggccgct gctgtacggc tctgcctatc
     1201 gtaaaggcga ttgggcctgg ctgcattacg gcctgctgtt cgacggcagc catcagcctt
     1261 cgcgacatga actgtttgat gatgtcgatg ctgtccttgt gtcaaaaaaa gacaattatg
     1321 caaattattc caaagaaatt ttaagcaggt atggccaata tttttatgat aactactatc
     1381 ttaaagacga aaattcagac tggcgcttgt atctgaaaaa gtcgtcagcc gtcaaataaa
     1441 cacgtttccg agtttttgaa taatgataag gtgtgtatta taaaacgttt tgaggttttt
     1501 aaataatgac aaggtgtatt ataaaacatt ttgagatttt taaataatga cagggtgtat
     1561 tataaaacat tttgaggttt ttaaataatg gcaagatatc ttataacagg gggggctggt
     1621 ttcctgggaa tcaacatggc cagattcctg ctcgataagg cacaggatgt gacttcactt
     1681 gacgtggcgc cgttcgatta tctggagcgc gacagggttc gcgaaatcac cggcgacatc
     1741 cgcgacgcgg caaaactcga cgaagccctt caggacgttc atgtcgtcat acacactgct
     1801 gcggcgctgc cgctgtataa aaagtcggaa attttttcga ttgatatcga cggtacgcgc
     1861 aacgtcatgg aacgggcata tgctgccggg gtgaagcgag tcgttcatgt ttcatctaca
     1921 gccgtttacg gcgtgccgga ccatcacccg atttttgaaa acgaccggct tgtcggcgtc
     1981 gggccgtacg gcgtcgcaaa aatatcagcc gaaaaaatct gtgccgaata ccgcgataaa
     2041 ggcatgtgcg tatccatcgt tcggccgaag tcgtttatag ggccggagcg gctgggcgta
     2101 tttgcactgc tttacgactg ggcaaaagac ggccgcaatt tcccgatgat aggcagcggc
     2161 aataaccgtt accagttgct tgacgtggaa gatctctgtc gggcaatatg gctgtgcgcg
     2221 acgctcgatg aaaaaatctg caacgatgtc tttaacatcg gcgccaaaga ttttacgacc
     2281 atgaaagagg attttcaggc ggttcttgac gacgccggtt tcggaaaaca tatcaagggt
     2341 tttccggctt ggccgatgat ttggacgctg cgaatcctgg aatttttcag gctttcaccg
     2401 ctttacaagt gggtttacga gaccgcctgc acagattcgt tcgtgagcgt ggaaaaagcg
     2461 gaaaaagtgc ttggattcac gcctgagttt tccaacaagg acgcgctgct gcgaaattat
     2521 cgctggtatt tgcagaatct tgccagcttt cagggccgca gcggcatcag ccatcgcgtc
     2581 ccgtggaaac agggcatact cgcgctagcc aaaaaaatgt tttgaacggt acggtttgga
     2641 ctttactgct gcggatttat cggacgtgat aaatccatag gcggttcatt attctcggtt
     2701 tgtacagtcg attccgctgg ttggccctgt ggagcggatt ctgaaatatc tgccaaggca
     2761 ggctgttgaa tgtcgtgata aaaaacggcg agggcggtat tgaagtatgg caccagccat
     2821 aaaaaaccta tgccgaatgt cagtatgcac aaaaatcccc agcctatgaa cgtcaggccc
     2881 agccagaata atttcagccg gtggccgtac atcagttcct tgctgcgttt aatcgcaccg
     2941 agtgcgccaa gttcgggatt gtcggcgtag acgtaataag ccatcgcata cgaatatgcc
     3001 gccataatgc ccggtataat cagaagcagc atccacagca gaacgaaaac aagcatgcac
     3061 caatacaggc aaaatgcgcc gataaaccgt ttaaatccgg cgaaaaaagt gccgaaagac
     3121 ggcatgccgc cgcgacccac acccagaaaa aacatcgcga agcccagcgt aaacggcccg
     3181 tttaaaagca gttgacccca gttgacttcc gcttccctgt attcatgcct ggcgaaaaac
     3241 ggcggataag tcgtaaaggc ggatattaat gatgttatta cgaaaaaacc gagcatatac
     3301 agtgcgcaca tgccccattt gccggcaagc gctgtgcgtg cttttgacct gatatcttta
     3361 catggcgtca tgccgccggt gcctttgatt tcagccgtcg tgccgttcat gattacgtaa
     3421 gccttcaccc agttttccat gccttccgcc cagacaaggt cattcggcag aaccctgcct
     3481 tcgcttatcc atg
//
LOCUS       DAONFI010000253         3490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_33092_length_3490_cov_3.852402, whole genome shotgun sequence.
ACCESSION   DAONFI010000253 DAONFI010000000
VERSION     DAONFI010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33092_length_3490_cov_3.852402"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLK08_08090"
     CDS             complement(<1..1080)
                     /locus_tag="PLK08_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPS53498.1"
                     /translation="MAGTNKLKTALIGSFRFRVLFCSVGAMVLTLVVTLMVGGIGMYH
                     ELEKNALVTIKNHVNFMAHDLNSYNDSCVAMVRTLAMCQEDGMFGRREETARVLQTIM
                     RADEDIYDLYVIYEPNADGQDEKYRNRPNHDATGRMNNCWYRRGGQIDYSPGVDMETS
                     LYYRGCKEQFLSGSSRKYMITEPYLYEGTLMYEITYPIVVNNKFVGIVGSDTLLDRMA
                     ADLRRRKQYESTEFLLLSAGGRIIACTDRPEYLTKTLDETPYAQLLTVLKDNVTRDEV
                     LKAKVGDTDDYYACASVPVGGWTLFMKVHSSDVLGGVHDMLLMQMIISIILVIILCVA
                     LAVVINSMLRPIFVATDVAQKVAQGD"
     gene            complement(1202..1987)
                     /locus_tag="PLK08_08095"
     CDS             complement(1202..1987)
                     /locus_tag="PLK08_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPS53499.1"
                     /translation="MAVINVLCIGDVVGSPGRAYIENNLPKLITDRQIDFVVCNAENI
                     AGGSGITPQLFNKLISSGVDVVTLGDHCFRKGDIYQLMGESDRIIRPANMPRNSVGKW
                     WTVAPTKSGSFQVGVTCAMGQMYMASCNSPWVAVDEALAKINDQTRISVIDFHAEASS
                     EKMGIGWYCNGRASVIFGTHTHTPTADAQILDGGTAFISDVGMTGPYDSILGRDKERV
                     LKFLTTAMPQRFDVAGGDVRMYCLLCGVQTETGKAVSVELIRV"
     gene            2161..2430
                     /locus_tag="PLK08_08100"
     CDS             2161..2430
                     /locus_tag="PLK08_08100"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPS53500.1"
                     /translation="MQQYRYIFIGDVQGVGLRWTARNIARNYEVTGYVRNLSDGTVEC
                     LIEGELREAEAVVEEILNRMDGFIRKTTCQTAPASGKFANFDVAY"
     gene            2476..2670
                     /locus_tag="PLK08_08105"
     CDS             2476..2670
                     /locus_tag="PLK08_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPS53501.1"
                     /translation="MECRKKANLEFCTCSYPGCGNKGVCCECLRYHLKNRELPGCCFP
                     DKDEKSYDRSFEAFAKAWGL"
     gene            2792..>3490
                     /locus_tag="PLK08_08110"
     CDS             2792..>3490
                     /locus_tag="PLK08_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53502.1"
                     /translation="MQVRFFVVPALISAAFLCGCNNKNDMSNAASTQPAAQTQAPHVT
                     QPADYTPELWVCGGMGSFQRIPNELYIAGILREKIEGAEPDVCITSGTQEKGPNSVMW
                     NLIDTPKWLDADAEKQAVWALRMFVDPKKDKGHFINKDGRTKIIVILAAPCTQTLPPK
                     STDKVDIEVYLMRDPRDKVPNQQFSAPRSEKYYRQFGTLYMVQSQPDMFPGLTGRKIP
                     GGIQGYHHFTGYWKP"
BASE COUNT          911 a          911 c          846 g          822 t
ORIGIN      
        1 gtcgccttga gccaccttct gggcgacatc cgtagcaacg aatatcggcc gaagcatgga
       61 atttatcaca accgccagtg caacacacaa aattatgaca agaattatcg aaattatcat
      121 ctgcatcaaa agcatgtcat gcacaccgcc aagtacgtcg gaagaatgaa ctttcatgaa
      181 aagcgtccag ccgccgactg gaacggaagc acaggcgtag taatcgtcag tatcgccgac
      241 tttagctttc agtacctcgt ccctggtgac attatcctta agcactgtca gcagttgggc
      301 gtaaggcgtt tcgtctagtg ttttggtcag gtattccggc cggtctgtac aggcgatgat
      361 tctgccgccg gcgctgagca gaagaaattc agtagattca tactgttttc gccgtcgtaa
      421 atcggcagcc atccggtcaa gcagcgtatc gctgccaaca atcccgacaa acttattgtt
      481 gacaactatc ggataggtta tctcatacat caacgtacct tcgtaaaggt acggttcggt
      541 tatcatatat tttcgcgaac tgccgctgag aaactgttct ttgcaacccc tgtaataaag
      601 gcttgtctcc atatccacgc caggcgaata atcaatctgg ccgccgcgtc tgtaccagca
      661 gttgttcata cggccggtag catcatgatt cggcctgttg cgatattttt catcctggcc
      721 gtcggcgttc ggctcgtaaa tcacgtacag gtcgtatata tcctcgtcgg ctcgcatgat
      781 agtctgtaga actctggcgg tttcctctcg ccggccaaac atcccgtctt cctgacacat
      841 ggcaagcgtg cgaaccattg ccacgcatga atcgttatag ctattaagat cgtgagccat
      901 gaagttgacg tgatttttaa tcgtaacgag ggcgtttttt tccaactcat ggtacatgcc
      961 gattccgccc accatgagcg ttaccacgag agtaagcacc atcgccccca ctgaacagaa
     1021 caatacgcga aatctgaaac tgccgataag tgcggtcttc agtttatttg ttcctgccat
     1081 aggctcagcc tcctgaaaac atgtaatgct tatcagtata tatcaaaccc gtcccgtccg
     1141 caatttcccg attggcaatg gcatatattt tttatccgtc cggaacggcg gctaatcgga
     1201 actaaacgcg gatgagttca acagagacgg ctttgccggt ttccgtctgt acgccgcaaa
     1261 gcaggcagta catgcggacg tcgccgcctg cgacgtcgaa acgctgcggc atagcggtag
     1321 tgaggaattt cagcacccgt tccttatctc tgccgagaat actgtcgtac ggccctgtca
     1381 tgccgacgtc gctgatgaac gcagttccgc cgtccagtat ctgcgcgtcg gcagtgggcg
     1441 tatgcgtatg agtgccgaaa atcacgctcg ccctgccgtt gcagtaccag ccgataccca
     1501 tcttttcgct cgacgcctca gcgtgaaaat caatcacgct tatgcgggtc tggtcgttaa
     1561 ttttggccag cgcctcgtca actgctaccc acggcgaatt acagctcgcc atgtacatct
     1621 ggcccatagc gcatgtaacg cccacctgga acgagccgga tttggtaggt gcaaccgtcc
     1681 accatttgcc gacggaattg cgtggcatat tggcaggccg gataatgcgg tcgctctcgc
     1741 ccatcagctg gtagatgtcg cccttgcgaa aacagtggtc gccgagcgta acgacatcca
     1801 cgccgctgct gatgagcttg ttgaaaagtt gcggggttat tccgctgccg ccggcgatat
     1861 tttcagcatt gcagaccaca aaatctatct gtcggtcggt tatgagtttc ggcagattgt
     1921 tttcgatata cgccctgccg gggctgccaa cgacatcccc aatacataag acattgatta
     1981 cagccattaa aaaatccttc tttccaggcc gacacatgtc gacagcagag attatagaac
     2041 ccttcgccgc acaaaacaag tcgatatcaa caagccgaaa tattataata ccatataatt
     2101 gcaatcgcca tgttgcggtt gccataacgt cgctcaagcc gacaaggatt tattgaaata
     2161 atgcaacagt atcgatatat tttcatcggc gatgtgcagg gagtcgggct tcgctggacg
     2221 gctcgcaata tcgcccgcaa ttatgaggtg accggctacg tgcggaattt atccgacggc
     2281 acggtcgaat gtctcatcga gggcgaatta cgggaggctg aggcggttgt cgaagaaatt
     2341 cttaaccgta tggacggatt tatacgtaaa accacctgcc agaccgcgcc ggctagtgga
     2401 aaatttgcaa attttgatgt cgcatattga ccgccggaat gtaaaattat tctcagccaa
     2461 atggagacga aaaatatgga atgcagaaaa aaggctaacc ttgaattctg tacgtgcagt
     2521 taccccggct gcggtaataa gggagtctgc tgcgaatgcc tgcgatatca tctgaaaaat
     2581 cgggaacttc ccggctgctg ttttcccgac aaggatgaaa aatcctacga caggtcattc
     2641 gaggcatttg ccaaagcatg gggactttag aacatgttaa acttgatatt gcgtaatact
     2701 gccacctacg acatcggctg gcaattaaaa cgaaatttcc aatttaacat gctctgacca
     2761 aaagtgtttg aaatcctcaa ggagacgtaa aatgcaagtg aggtttttcg tcgtaccagc
     2821 attgatatca gccgcatttt tatgcggctg caacaataaa aacgacatga gcaacgccgc
     2881 gtcaacacag ccggcggcac aaacgcaagc tccccacgta acccagccgg ctgactatac
     2941 gccggaatta tgggtctgcg gcggcatggg cagttttcag agaattccga atgaattata
     3001 catcgccggc attttaaggg aaaaaatcga aggcgccgag ccggacgtat gcataaccag
     3061 cggcactcag gaaaaaggcc ccaacagcgt catgtggaat cttatcgaca cgccgaaatg
     3121 gctggacgcc gatgctgaaa aacaggctgt atgggcgttg cgaatgtttg tagaccccaa
     3181 aaaagacaag ggacacttca taaacaagga cggcagaaca aagataatcg taattctggc
     3241 tgccccctgc acgcagacgc tgccgcccaa aagcaccgac aaggtcgata tagaagttta
     3301 tctgatgcgg gacccgcgcg ataaagtgcc caatcagcag ttttcggctc cccgcagcga
     3361 aaaatattac cggcagttcg gcacgcttta tatggtgcaa tcccagcccg acatgtttcc
     3421 agggcttacc ggcagaaaaa ttcccggcgg catacagggt tatcaccatt tcacgggtta
     3481 ctggaagccg
//
LOCUS       DAONFI010000254         3483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_33207_length_3483_cov_3.505251, whole genome shotgun sequence.
ACCESSION   DAONFI010000254 DAONFI010000000
VERSION     DAONFI010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33207_length_3483_cov_3.505251"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="PLK08_08115"
     CDS             <1..1492
                     /locus_tag="PLK08_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPS53503.1"
                     /translation="PSLEQEIADALGDMNIDDLIAEEEKSHSAARIKSDKIIKGKVMD
                     INGEDIFVNIGSRQDGVIGTDQFRDGELPAVGDEIEVVIEGVIPGEGLIRLSRQGAVQ
                     AAAWDTIQKGLKVEGRVTGFNKGGLDVDINGIRAFMPISQIALERIETEAMGEFVNQR
                     LECEVTEVDRANSNVIVSRKILLQREVDAKAEELWKNIHEGMIVQGTVRSIMPYGAFV
                     DIGGIDGLLHIKDMAHSRVEKPQDIVQLGQKLELKVISLDRENNRVGLGLKQNLADPW
                     DSAAVNYPADSLVTGKVVKLMDFGAFVELEPGVEGLVPISELSFGRRIGHPREVLNVG
                     DMVKLRVLKIEPENHRMSLSLKRIGDDPWVGAEVRWPAGSVAEGTVTRITEFGAFVSL
                     GEGVEGLVHISQLSPKRVNSAHEVVREGQAVKAVVLEVDEQRRRISLSLKKLQEDAAG
                     QAAAESQGTMDDLKRVNNTETKKSKKRKGGLDGGTARTPFGELRLG"
     gene            1503..2312
                     /locus_tag="PLK08_08120"
     CDS             1503..2312
                     /locus_tag="PLK08_08120"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPS53504.1"
                     /translation="MIILGNMERQDVCEAVESASKRLEKRGLLGGVFPHTVAVDDLPK
                     SDMCLVFGGDGTLLSAGRRLARLGIPFIGVNMGKLGFLAEYNLDELYRFLPALVEGKI
                     KPVERIMLDVCCCDFCSLAVNDLAISAGAPFRMIDLSFSHDENMLARFRGDGLVVATP
                     TGSTGYNLSVGGPVLQPTLDAIAVSPIAPHTLSLRPIVLDTFAPVHINVMGANAGTTL
                     IIDGQISHKLCAGQSIVVRKADVTLKIFPHPERHYTQLLSNKLKWGQSPHH"
     gene            2316..>3483
                     /locus_tag="PLK08_08125"
     CDS             2316..>3483
                     /locus_tag="PLK08_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53505.1"
                     /translation="MTSTAIHEISSKPPKLFSSYLAKTCDWLALVVAFAACILFAVRP
                     VGNADIGYHLSFGEHLWKTGSFVRESNFVYVKTDDKTDIGPGEWWDDNGVMRIVPVSW
                     GTQAVMWWFYDNWGFDGIFVLTLILVGSILGLTGWLCVRLGLPASLGGVVILLCSFAG
                     YERFYIRPELFSYIFMLCNLHVMLGFYMGRLDVLSWKKIFLLAVIQIFMVNVHSYFLL
                     GWLFVFLPLCETAILWLWQKYRKGDAAEYLPVLRRLAIAFFVVIAASFVSPSPWRLFM
                     FPFEMLLYLRQAAQDTQIPINSITELFSPFDNDNWQQTSIFHTYVISLGLVGAGCICG
                     LYRRKWLMVGLMLLMVAQSLTMRRNILPSCVVMLPMATECIWSIISPWKNNVRKV"
BASE COUNT          824 a          772 c          979 g          908 t
ORIGIN      
        1 cccgtcgctt gaacaggaga tagccgacgc gcttggcgat atgaatatcg acgaccttat
       61 cgccgaggaa gagaaaagtc attctgccgc gcgaattaaa agcgacaaaa tcatcaaagg
      121 taaggtcatg gacatcaacg gcgaagatat ttttgtaaat atcggttctc gccaggatgg
      181 cgttataggg acagaccaat ttcgtgacgg cgaattgccg gctgtcggcg atgagattga
      241 agtagtgata gaaggcgtga ttccaggcga aggtcttatc cgtctgagcc gccagggtgc
      301 cgtgcaggca gccgcatggg ataccattca gaaaggcctt aaagtcgaag gacgcgtgac
      361 tggttttaac aagggcggcc tcgatgtgga cataaacggc attcgtgcat ttatgccgat
      421 tagccagata gctctcgaac gcatagaaac cgaagcgatg ggcgaatttg ttaatcagcg
      481 gcttgaatgc gaagtcacgg aagttgaccg cgcaaattcg aatgtaatcg tgtcgcggaa
      541 gattctgctt cagcgcgaag tcgacgccaa ggctgaagaa ctgtggaaaa atatccatga
      601 aggcatgatt gtacagggta cagttcgcag tatcatgccg tatggcgcgt ttgtcgatat
      661 tggcggtata gacggcttgc tgcatataaa agatatggcg cattcccgcg ttgaaaaacc
      721 gcaggatata gttcagctcg gccagaaact cgaactcaag gttatcagcc tcgaccgtga
      781 aaacaaccgt gtgggcctcg gcctcaagca gaatctggcg gatccgtggg attcggcagc
      841 ggtgaattat ccggcagatt cgttagtgac gggcaaagtc gtcaaactga tggatttcgg
      901 cgcgttcgtg gaacttgagc ctggtgttga aggacttgtg ccgataagcg aactctcatt
      961 cggacgacgt atcggccatc cgcgtgaagt gctcaacgta ggcgatatgg ttaaactccg
     1021 cgttcttaaa atcgagccgg aaaatcaccg tatgagtctg tcgcttaaac gcataggcga
     1081 tgatccgtgg gtgggcgccg aagtacgctg gccggctggc agtgtggctg aaggtacggt
     1141 tacccgcata accgaattcg gcgcttttgt aagtttgggc gagggagttg aaggacttgt
     1201 tcacatcagc cagctttcgc caaaacgcgt gaattcggca catgaagtcg tgcgtgaagg
     1261 ccaagccgta aaagccgtcg ttctggaagt cgatgaacag cggcgtcgca tttcgctttc
     1321 acttaaaaaa cttcaggaag atgccgccgg ccaagctgct gccgaatcgc agggaacgat
     1381 ggatgatctc aaacgggtca ataacaccga aacaaaaaaa tccaaaaagc gcaagggtgg
     1441 acttgacggc ggtacggcgc ggacgccttt tggagaattg cgactcggat agaggtttga
     1501 aaatgattat tctcggcaac atggaaagac aggatgtctg tgaagcggtg gaatccgcca
     1561 gcaaaaggct tgaaaaacgt ggccttcttg gcggcgtgtt tccacatacc gtagcagtcg
     1621 atgatttgcc gaaatctgac atgtgcctag tgttcggcgg tgacggcacg cttctttccg
     1681 cgggcaggcg gcttgcgcgg ctgggcatac cgtttatcgg cgtgaacatg ggcaagctgg
     1741 gatttctggc tgaatataat ctggatgaac tttaccgctt tcttcccgct cttgttgaag
     1801 ggaaaataaa acccgttgag cgtataatgc tggacgtgtg ctgctgtgat ttctgcagcc
     1861 ttgccgttaa cgatctcgca atatcggcag gagcgccttt tcgcatgata gacctgagtt
     1921 tttcgcacga tgaaaatatg ctggcacgat tccgcggcga tggcctggtg gttgccacgc
     1981 ctacaggatc gacaggctac aatctttccg tcggcgggcc ggtgcttcaa cccactcttg
     2041 acgccatagc cgtctcgccc atagcccctc atacgctgtc attacggcct atagtgctgg
     2101 acacatttgc gccggtacat ataaacgtca tgggcgcaaa tgccggcact acgctgataa
     2161 tcgacggcca gatatcgcat aaactctgtg ccggccaatc gattgtcgtg cgaaaagccg
     2221 atgtgaccct caaaatattc ccccatcctg aacgtcatta tacgcagctg ctttccaata
     2281 aactcaaatg gggccaaagc cctcatcatt gatatatgac ttcaacggct atccatgaaa
     2341 tatcgagtaa gccgccaaaa ttgttttcgt cgtacctggc gaaaacatgc gattggctgg
     2401 cgcttgtggt tgcgtttgcg gcgtgcattc tttttgccgt acggcctgtg ggcaatgccg
     2461 atatcggcta tcatctttca ttcggtgaac atctctggaa gacgggcagt tttgtcagag
     2521 aaagcaattt tgtttatgtg aaaaccgatg ataaaactga tatcggccct ggagagtggt
     2581 gggacgacaa cggcgtgatg cgtattgttc ctgtcagttg gggcacgcag gctgttatgt
     2641 ggtggtttta cgacaattgg ggatttgacg gaattttcgt gttgaccctg attctcgtcg
     2701 gcagcatact cggattgacg gggtggctgt gtgtgcggtt gggattgccg gcttcgttgg
     2761 gtggcgtggt gattcttctt tgcagttttg ccggctacga gcgattttat ataaggcctg
     2821 aacttttttc ttatattttc atgctgtgta atctgcacgt gatgctgggg ttttatatgg
     2881 gccggctgga tgtgttgtcc tggaagaaaa tatttttatt agccgtgatt cagatattta
     2941 tggtcaatgt acacagttat tttcttcttg gctggctgtt tgtatttttg ccgttatgcg
     3001 aaactgcgat tctgtggctg tggcaaaaat atcgtaaagg cgatgctgcg gaatatctgc
     3061 cagttttgcg gcggcttgcg attgcctttt ttgtcgtgat agccgcaagt tttgtcagtc
     3121 ccagcccgtg gcgattattt atgtttccgt ttgagatgct gctttatttg cggcaggctg
     3181 ctcaggatac tcaaattccg ataaacagca taactgaatt gttcagcccg tttgataatg
     3241 acaactggca gcagacgtcg atttttcata cttacgtgat ttcactcgga ctggtcggag
     3301 caggatgtat atgcgggcta tatcgccgca aatggttaat ggtcggactt atgctgctta
     3361 tggttgcaca atcgttgacc atgcgaagga atattttgcc gtcgtgtgtg gtaatgcttc
     3421 cgatggcgac ggaatgcatc tggagtatca tatcgccgtg gaagaacaat gttcgtaaag
     3481 tgg
//
LOCUS       DAONFI010000255         3436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_33943_length_3436_cov_3.588583, whole genome shotgun sequence.
ACCESSION   DAONFI010000255 DAONFI010000000
VERSION     DAONFI010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33943_length_3436_cov_3.588583"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1015
                     /locus_tag="PLK08_08130"
     CDS             212..1015
                     /locus_tag="PLK08_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53506.1"
                     /translation="MLKTIPCMISLMVVAAGLICQPSSAGQLADVVCSYKAGKNPNTG
                     YTDGNAILQPATSDDDTSGIVSLGQWEESSLRGSPVGIIVGFSTSIANLDGNDLLIVG
                     NAMTSWYEPGYVEVAMETSGSGATADGWEDETFYLLKPSNYDQVGDPRTNALSITWDQ
                     TAFDDGDNPYTDDAWSDQSSLTGYADVTVGGDAMDISWAIDASGESVTLSSIAYIRIR
                     TVTNSTAGTFGYFSTDLDYIEYMHVVPEPFCTAMLVATATAMLIRRRRF"
     gene            1109..1936
                     /locus_tag="PLK08_08135"
     CDS             1109..1936
                     /locus_tag="PLK08_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53507.1"
                     /translation="MKNRYDNPAFTLIELLVVVAIISLLVAVIFPSLSSAKELARKAA
                     CKMSIRCLQLGNLQYEQDNDGFFAPATDSGKNLRRWFGRRTTASPNSTPFSIEEGSLL
                     EYLPGRAVRNCPSFTDFNSGFETGCGGFGYNQTFVGSYIKLVGQDEAGRGIYENGNSS
                     PSDSGNQADNFCTAAETVAFTDSAFANNGITEYSFCEPPRGAMQCETWSATLVHRPSI
                     HFRHMETANVVWLDSHVNEEKLTFSNNSAGSAYGGTKPIDLNIGFFGENNYELFDLE"
     gene            complement(2742..3167)
                     /locus_tag="PLK08_08140"
     CDS             complement(2742..3167)
                     /locus_tag="PLK08_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53508.1"
                     /translation="MSESLNNGMETRAVEGACIVVCANKDAVRQLSSSLVARGAGRIL
                     SYDNLSDFATNIPVFNVDMVVLSGDVTPQITYRMLRWIRRQWRNCVSVVVGNLGQRDV
                     EIAARRGGAFYLATPTAECDWDIILDGVRQNREYCMSQN"
BASE COUNT          931 a          864 c          811 g          830 t
ORIGIN      
        1 aacgcttcaa gctatgattg gccgacgtat ttcgcaatag acagcgttac cgtgcccgaa
       61 ccattgacgc tctgcctgct aacgttgggc agcctggcaa tgctgcggcg taaaaaaggt
      121 aaaaaataat gctccgtcgt aaaaccaaac cgcgcacaac aaatatatcc ggttcagttt
      181 tgcgacaaaa tatgaatgag gaatactttt tatgctcaaa actattccat gtatgatttc
      241 gttgatggtt gtggcggctg gcctgatttg ccagccgtcg tcagccggcc aactggctga
      301 cgtggtatgc agctataaag ccggcaaaaa ccccaataca ggttacacgg atggcaatgc
      361 aatattacag cccgccactt cagatgacga caccagcggc atcgtttcac ttggccagtg
      421 ggaggaaagc agcctccgag gttcaccggt cggcataatc gtgggtttct ccacttccat
      481 tgccaatctt gacggcaatg acctgcttat agtaggcaac gcaatgacaa gctggtatga
      541 gcctggctat gttgaagtgg caatggaaac gtccggcagc ggcgcaacgg cagacgggtg
      601 ggaggacgaa accttctacc tgctcaagcc gagcaactac gaccaggtag gcgatccccg
      661 cactaacgcc ctgtcgataa catgggacca gacggcattc gacgacggcg ataatccgta
      721 taccgacgac gcatggtcag accagtcgtc attgaccggc tatgcggatg tgaccgtcgg
      781 cggcgatgcg atggatatca gctgggcaat agacgcttcg ggagaatctg taaccctctc
      841 gagcatagct tatattcgaa tacgcaccgt gacaaacagt acggctggca cattcggcta
      901 tttttcaaca gatttggatt atattgaata tatgcacgtc gtgcctgagc cgttttgtac
      961 ggcgatgctt gttgcgacgg cgacagcaat gctcatccgc cgcagacgtt tttagaacat
     1021 gaaaatattt ttatttttgg gcgatattga ctcattttac ttttcggtag caagggagcg
     1081 ggcctgaacc acccgctccc gacagattat gaaaaacaga tacgacaatc cggcattcac
     1141 gcttatcgaa ctacttgtcg tagtggcgat aatctccctg ctggtcgccg tcattttccc
     1201 cagcctttcc tctgcaaaag agctggcccg caaagccgcc tgcaaaatga gcattcgctg
     1261 ccttcaactg ggcaatctgc aatatgagca ggataacgac ggctttttcg caccggctac
     1321 cgatagcggc aaaaatcttc gccgatggtt cgggcggcgc accaccgcca gtccgaattc
     1381 aacgcccttc agcatagaag aaggctcgct gttggaatat ctgcccggcc gcgcagtcag
     1441 gaattgtccg tcatttacgg atttcaacag cggttttgaa acggggtgcg gcggcttcgg
     1501 atataatcag acattcgtgg ggagttacat aaaactcgtc ggccaggatg aggctggacg
     1561 aggcatatac gagaacggaa attcatcgcc gtctgactcc ggaaatcagg ctgacaattt
     1621 ctgcacggcg gctgaaaccg tggcgttcac cgatagcgct ttcgccaaca acggcataac
     1681 ggaatacagt ttctgcgaac cgccgcgcgg agcaatgcaa tgcgaaacat ggtcggcgac
     1741 gctggtacac aggccgtcga tacatttcag gcatatggaa acggcaaacg tcgtatggct
     1801 ggattcacac gtcaacgagg aaaaattgac tttctcgaac aactccgccg gcagtgcata
     1861 cggcggaaca aagccgatag atttgaacat aggtttcttt ggcgaaaata actacgaact
     1921 tttcgacctg gaataaaaaa gccgacatga ctttatttaa aaatggtttt caacaggatt
     1981 tcctttaaaa accgttttta aaagtcatat cggctttttt gtgcgcaaaa aaaaggggcg
     2041 acactcggaa tgctcagttc cgtttatcgc ccttcgcctt agctacagaa gcctctccgc
     2101 tatcgatgca ttacgaagtt ccggaggagg agaaaaccgg aatccctcgt gggcgctcat
     2161 ctccacaagc tttcgcaagg ggctcaatag caacctctgt ctctaaaatc ggaatctctg
     2221 atgcaatgaa ggcataaatt aggatgttgt tgagaagaaa ggagttttca ccatccggac
     2281 tgcctttatt ttcaccgggc gctcatctcc ggagaaaccg caccgaagag actctagtgt
     2341 tttgagaggt ctcgcctctc gtcagtcgag agactttcaa tttcgcaaac tattgggttg
     2401 attggtttgg ttgggaaagg agaaagttca gcgaaatcaa aagcctttgg ctcctcgatc
     2461 tgccacttac agtggttgaa tattcgattg ttatatacag taagctttct gagcaaagct
     2521 ttaacatttg tcgttacgta tgaagatatg catctgccgt gccaaaaccg ttattttttt
     2581 tcacggcgag gataaaaaga cataacatac tgaaaaaaca atacgttacg agtataggta
     2641 tttagaaaaa tatttatcga cgatagtttt tattctttta cgactgtcgc gtcaatcaca
     2701 tgcagccgtt gcgaatgtcg caatatgcta cacctgatca atcaattctg cgacatgcag
     2761 tattcgcgat tttgccgcac gccatcgagt ataatatccc aatcgcactc agcggtgggc
     2821 gtggcaagat agaacgcccc gccgcggcga gccgcaattt cgacgtccct ctgccccaaa
     2881 tttcccacta ctacagatac gcagtttctc cactgccgcc tgatccatcg cagcatccga
     2941 taggtaatct gcggtgtgac atcaccggac aaaactacca tatctacgtt gaaaacgggg
     3001 atatttgtcg caaaatcgga caaattgtca taggacagaa tcctgcctgc cccacgcgca
     3061 accaggcttg aagaaagctg ccgtaccgca tccttattgg cacaaacaac gatacacgcg
     3121 ccttcaacag ctctggtctc cataccatta tttaggctct ctgacatctg catcgctcca
     3181 agaatcaact tagtcaacac accacttaac gcaatgctct ctctcgtcgg ctaagtattg
     3241 gcacgagatg ccggaattaa ttgatgcaat taacatgcca aacacgaaca gaattttata
     3301 gaacagcctc aaaagatttc cgaaaagaaa ttttttgagg ccgctctata acgctcaaca
     3361 tgatgtcaga aattttgttg ctagaaccta tcccaaaaac atgggcgtcg ccgaaatcga
     3421 gcaattttgc tggctg
//
LOCUS       DAONFI010000256         3424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_34114_length_3424_cov_4.173345, whole genome shotgun sequence.
ACCESSION   DAONFI010000256 DAONFI010000000
VERSION     DAONFI010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34114_length_3424_cov_4.173345"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1064
                     /locus_tag="PLK08_08145"
     CDS             279..1064
                     /locus_tag="PLK08_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HPS53509.1"
                     /translation="MNENIVNFQLFSPLHWGLMFFTVLAPLLLGMAVNRAGGAKTAMI
                     VARILVVMLLGGEVVQDCYSFCTQPSFQDFYRRSLPFHLCDFGMILTAVYMWCYPRRL
                     QRIFEVAFFWSVAGAIPAMLTPDLPADYPSFGFWQFYVSHMLIVVCNVYAIVSLRYRP
                     RAAGILWAWLATNVVFVIVAAINLLLRSNYFFICHPPQTAMPLFFLPWPWYVVFMEFL
                     TPVFFLVVYLPFGLAAGMVRRGICGLHSAGRNVIIARDSDKRK"
     gene            1064..2215
                     /gene="alr"
                     /locus_tag="PLK08_08150"
     CDS             1064..2215
                     /gene="alr"
                     /locus_tag="PLK08_08150"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPS53510.1"
                     /translation="MMFDDRFKYVTAEISAGALVENIRLCRSLVGDSQVWVLIKADCY
                     GHGLKLIWPVLAEYADGMVAATPGEAMTIREFGYDGPVLVLFPSGCEALSPDQADCVD
                     ELIRTDTHLTITCSAEADYLARRAARVGRPARIHIMVDSGMTRSGVLPCAAESLLTHV
                     NADAHLKLTGIYTHLATADEADKSFAHQQLDTFDATLEKCCLTAGCGIMRHAANSPGL
                     IELPRCRYDMVRLGLSLFGYHSSFDMENRLPLRPCLKLKSTIMHTSAVPAGSKCGYGC
                     TYVCKRDTLIGLVPIGYADGYLRQFSNRASMIVRGQVVPVIGRVSMDQTILDLTDVSG
                     AAAGDEAIIISNIASDKNSVERLAVIGDTIPQVVTSNLNPIRIRRVLTD"
     gene            2424..3278
                     /locus_tag="PLK08_08155"
     CDS             2424..3278
                     /locus_tag="PLK08_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53511.1"
                     /translation="MQRNVLSSLVILVLLTASPVMAIVTNDTATSATDPSNYSSYNLS
                     SLDWDGVYQFGTSAGKTMTAVGSYWLISSRHYTLSAGNTFTQDGVTYTIQEVVNHSAS
                     DDPNHDYDADLTLIRVDKALSTYYDIYDGASITNKVVAMIGTGHAAGTVSSTSYTWSD
                     STSRVRRWGTNKVDFAYSDYAMGTYTEDILGAYFTLGNTTYEAGAADHDSGGGWLVYD
                     TLSGTWELVGLNIAVMGTTGAYTGSVAIDLRDYADWIDSVISVPEPVTVAILTLGGCA
                     VLLRRRRK"
BASE COUNT          785 a          764 c          918 g          957 t
ORIGIN      
        1 gtatagataa tcgttttttt aaaaatcgct gcggcagcca cagtgttgca tattgcgtct
       61 ttttttaaat cgagaatcgt atatagatag ttattttaaa aaattgctgc ggcagccaca
      121 gtgttgtata ttgcgtcttt ttaaaaatct agaatcgtat atagatagtt atttataaaa
      181 aaaactgccg cgcatttaaa aaaatcgaga gtccgtaaaa atagtttttt ttaaaaaaaa
      241 ctgcctgtga aaaatagcct cgaaatcgta aggctgcgat gaatgaaaat atcgtaaatt
      301 tccaactctt cagccctctg cattgggggc tgatgttttt tacggttttg gcgccactgc
      361 tgctcggcat ggcggttaac cgtgccggcg gcgcaaagac ggcgatgatt gtcgctcgca
      421 tacttgtggt gatgctgctc ggcggcgaag tcgttcagga ttgctacagc ttctgtactc
      481 agccgtcgtt tcaggatttt taccggcggt ctctgccgtt tcacctctgt gatttcggca
      541 tgatactgac ggctgtttat atgtggtgtt atccgcggcg gctgcagagg attttcgagg
      601 tggcgttttt ctggtccgta gccggagcga taccggctat gcttacgccg gatttgccgg
      661 cggattatcc gtcattcggc ttctggcagt tttacgtatc gcacatgctg attgtggtat
      721 gcaacgtcta tgcgattgtg tcgttgcgat accggccgcg tgcggcggga atattgtggg
      781 cgtggctggc gacgaacgtc gtattcgtaa tcgtggcggc gataaatctg ctgctgcggt
      841 cgaattattt ttttatatgt catccgccgc agaccgctat gccgctgttc ttcctgccct
      901 ggccgtggta cgtggttttc atggaatttc tgacgccggt ttttttcctt gtcgtatatc
      961 taccgttcgg gttggcggcg ggaatggtga gacgcgggat atgcggctta cattcagccg
     1021 gcaggaatgt tataattgcc cgcgattctg acaaaaggaa gtgatgatgt ttgacgacag
     1081 gtttaaatac gttaccgcgg agatatccgc cggcgctctt gttgagaata tccgtctgtg
     1141 tcgcagcctt gtcggcgatt cgcaggtatg ggtgctcata aaagccgact gttacggcca
     1201 cgggctgaaa ctcatctggc cggttttggc ggagtacgcc gacggcatgg tggcagctac
     1261 gcctggcgag gctatgacga tacgcgaatt cggttatgac gggccggtgc tggttctgtt
     1321 tccgagcggc tgcgaggctc tttcgccgga ccaggcagac tgcgttgacg aacttatccg
     1381 cacggacacg catctgacga taacgtgttc cgccgaggcg gattaccttg cccgtcgtgc
     1441 cgccagggtt ggcaggcctg cacgcattca cataatggtg gattcaggca tgactcgaag
     1501 cggcgtattg ccgtgcgctg cggaaagcct ccttacgcac gtcaatgccg acgcacatct
     1561 gaaactgacg ggcatatata cgcatctggc gaccgccgac gaggctgata agtcttttgc
     1621 tcatcagcag ttggatactt ttgacgccac tcttgaaaaa tgttgcctga cggccggctg
     1681 cgggattatg cgtcatgccg ccaattcgcc agggctgata gagcttccgc gatgtcggta
     1741 tgatatggtg cggctgggac ttagcctttt cggttatcat tcgtcgttcg atatggaaaa
     1801 ccgtctgccg ctgcggccgt gccttaaact caaaagcacg ataatgcata ccagtgctgt
     1861 gccggctggc tcaaaatgcg gctacggctg cacttacgtc tgcaaacggg atacgctcat
     1921 cggccttgtg ccgataggct acgccgacgg atacctgcgg cagttcagca atcgggcaag
     1981 catgattgtc cgcggccagg ttgtgccggt tatcggcaga gtgtccatgg atcagaccat
     2041 cctcgacctg acggatgtct ccggggcggc tgcaggcgat gaagcaataa tcatatcaaa
     2101 tatcgctagc gataagaaca gcgtggaacg tcttgctgtt attggagata cgattcctca
     2161 agtcgttacc agcaatctga atccaatccg catccgtcgg gttctgaccg attaatccgg
     2221 ttatggtaca aaaaaattgt gttggttatg caataaatta taatataatg agttaatcgc
     2281 agaagagtct cttttcgctc agggaaggga cgctataaaa aaatgctcat agctcgaaat
     2341 ggaggagttg cgcaatgccc cacggcgggg cgagctcaac gcgcgtagta ttgtgcgctt
     2401 gttttataag ttagggttgg actatgcaac gcaacgtttt gagcagtctt gtcattctgg
     2461 ttttgttgac agcctcgccg gtaatggcaa ttgttacaaa tgataccgca acaagcgcaa
     2521 cagacccttc aaattattca tcttataatc tttcttccct ggattgggat ggagtgtatc
     2581 aattcggtac tagtgcgggc aaaaccatga ccgccgttgg gtcatactgg ctgatatcga
     2641 gcagacacta tacgctgagc gccggaaata cgtttacgca ggatggcgtg acttatacca
     2701 ttcaggaagt ggttaaccat tctgcatcag atgacccaaa tcacgattat gatgccgacc
     2761 ttacgttgat tcgcgtggat aaggcacttt caacatatta cgatatttat gatggcgcct
     2821 ctattacgaa taaagtggtt gccatgattg gtacaggcca cgccgcaggt actgtatctt
     2881 ccacttcgta tacgtggagc gattcaactt ccagagtaag acggtggggt actaataaag
     2941 ttgattttgc atacagtgat tatgctatgg gtacttatac cgaagatatt ttaggtgctt
     3001 attttacatt gggcaatacc acttatgaag ccggtgccgc agaccatgat tccggtggtg
     3061 gttggctggt ttatgataca ttaagcggta cgtgggaact ggttggcctt aatattgcag
     3121 ttatgggaac tactggagca tataccggtt ctgttgccat tgacctgaga gattatgcag
     3181 attggataga tagtgtcatt tccgttcctg aacctgtaac ggttgcgatt ctgacgcttg
     3241 gcggatgtgc agtattactg cgtcgtcgca ggaaatgaaa cgccaaacat aacgtaaaaa
     3301 ttcaagcctc gcatctgcgg ggcttttttt atgaaaacct atcccatgat acaatgagct
     3361 tacggtgggt gtctgttgat ttttgcggat tcgttaaaaa aatatcgatt ttgtgtgcaa
     3421 ttct
//
LOCUS       DAONFI010000257         3422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_34142_length_3422_cov_5.061776, whole genome shotgun sequence.
ACCESSION   DAONFI010000257 DAONFI010000000
VERSION     DAONFI010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34142_length_3422_cov_5.061776"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1206
                     /locus_tag="PLK08_08160"
     CDS             217..1206
                     /locus_tag="PLK08_08160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPS53512.1"
                     /translation="MAKNNNIAVTGVWLPFEEKIARLYQQIEDLEHEQIKTGRDYLSE
                     IRQIRAQYVSLLKKIYDNLTPWETVQVARHPKRPLTRDYIDMMVKNFVELHGDRRFGD
                     DKSIVGGFGRIGTEKVLFVGHHKGRDTKEKIAHNFGMSQPEGYRKALRLMRLAEKYSL
                     PVVTFIDTPGAYPGVGSEERGVAEAIAVNLMEMSRLRVPVVCIVIGEGGSGGALGIAV
                     GDRVAMLQYSYYSVISPEGCASILWRTGEKAPEAAQALKLTAPSLRDLGIINDIIEEP
                     LGGAHRNPAEAAHNLEQYIVSTLRSLKRKKIETLLNSRYESWRNKGKILQNAE"
     gene            1642..2316
                     /locus_tag="PLK08_08165"
     CDS             1642..2316
                     /locus_tag="PLK08_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53513.1"
                     /translation="MSSSASAVSVAEWDVAGNASNASKVSVLSTANGVSATDITATGV
                     NVLRAGSSKYAGVFCSNHWATSEVRDTSKYYSWSITDTVGYDLTSIVMSLSRGKYEDN
                     DNPDEPYGASKWDLYVNDQLVGTIDLDNYDGNGTAAQPDEPIVWTVDLTSVLSASLYV
                     FKLYGYSDEGGSDYAGLNESWYSHSGTNVTLYGSAVPEPLTVLSVMLGCSGLGYYLKK
                     RLSNIF"
     gene            3038..3232
                     /locus_tag="PLK08_08170"
     CDS             3038..3232
                     /locus_tag="PLK08_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53514.1"
                     /translation="MVRKINTIPFGAEIPPDLAEKFDEVIDESGWTKKRALAAAVRAF
                     CKLDESTRADLYKKAYDKLK"
BASE COUNT          918 a          733 c          841 g          930 t
ORIGIN      
        1 acggttgacg tgttttgaat tgtttttaaa accgttaaat tacggttgac gtgttaaaaa
       61 ttgtttaaaa accgttagat tacggttgac gtattaaaaa ttgtttaaaa actgttatat
      121 tacagtagac gtgttaaaaa tttaaaaaat tccggcaggc cagaatgcct gctttatccg
      181 tccgggccag agttgtccgt gttgaggatt tggaaaatgg caaaaaataa caatatagct
      241 gtaaccggtg tatggctgcc ttttgaagaa aaaatagcgc ggctttatca gcagatagaa
      301 gacctcgaac atgagcagat aaagactggc cgcgattacc tgtctgaaat ccgtcagata
      361 cgcgctcagt atgtttcact gctcaagaag atttatgaca atcttacgcc gtgggagacg
      421 gtgcaggttg cccgccatcc caagcgtccg ctgactcgcg attatatcga catgatggtc
      481 aagaatttcg ttgagcttca cggcgacagg cgctttggtg acgacaaatc gatagtcggc
      541 ggcttcggcc gcatcggcac tgaaaaggta ctgttcgtcg gccatcacaa aggcagggat
      601 accaaggaaa aaatcgccca caatttcggc atgtctcagc cggaggggta tcgcaaggcg
      661 ctgcggctga tgagacttgc tgaaaaatat tcactgccgg tcgttacatt tatagacacg
      721 cccggtgcgt atcctggagt aggttctgaa gagcgtggcg tggcggaggc tatcgcggtg
      781 aatcttatgg agatgtcgcg gctgcgcgtg ccggtagtct gcatagtcat cggcgaaggc
      841 ggcagcggcg gtgcgcttgg catagcggtc ggcgacaggg tagcgatgct tcaatattcg
      901 tattattccg tcatcagtcc ggaaggctgc gccagcatac tttggcgtac aggcgaaaaa
      961 gcgccggaag cagcccaggc actcaagctc acagccccca gcctgcgaga ccttggcata
     1021 ataaatgata ttattgaaga accgctcggc ggagcgcatc gaaacccagc cgaggcggct
     1081 cacaacctcg aacaatatat cgtctccact ctccgcagcc tcaagcggaa aaaaatcgaa
     1141 acgcttctga acagccgcta cgaatcatgg cgaaacaaag gcaaaatttt gcagaatgca
     1201 gaataatgca tgaaaactac attggccgcc gcttattggc gatgcattca tgtgaaggta
     1261 atttttaata tgtccttcgt agttctacgg tttttaaacc gtagatgaaa gtatccgccg
     1321 tgaggctgac aggcgaaagc ctgatgaata tgatgctacg ggttttaacc cgtagagatt
     1381 cactatgtcc ttcgtaattc tgcggtttta aaaccgtgga gattcccttt ttattaccct
     1441 cgcaatgcca atatgttatg tctaaaacag ggtatgcttc ttgccgtgcg ggaatttttg
     1501 taaaaactac agaaccggcg cgatttgaat tgattttgag ctgttttggc ttataatcaa
     1561 caagattaat taagagaacc atatatcatg aaaacgttaa aaacattttt gtttttcagt
     1621 gttggcatcg cagtttgtgt gttgagttca tcagccagcg cggtatcggt tgccgaatgg
     1681 gacgtggcag gcaatgcgtc caatgcctcg aaagtgtcgg tgttgagtac ggctaacggc
     1741 gtttccgcga ccgatattac cgctaccggc gtcaatgtcc ttcgcgcggg cagttcaaaa
     1801 tatgccggcg ttttctgctc gaatcactgg gcaacgtcgg aagtccgcga cacgtcaaaa
     1861 tattattcat ggtcgataac cgatactgtc ggatacgatt tgacctcgat agtcatgtcg
     1921 ctttcccgcg ggaaatatga ggataacgac aatcctgatg aaccgtacgg cgcatccaag
     1981 tgggatttgt acgtaaacga tcaattggtc ggaacgattg atttggataa ttatgacggt
     2041 aatggtacgg cggcacaacc tgatgagccg attgtatgga ctgttgattt gacgtccgtt
     2101 ttatcggcgt cgctttacgt gttcaaactt tacggatatt ccgatgaagg cggctcggat
     2161 tacgcagggc tcaacgaaag ctggtatagc cacagtggta caaatgttac tctttacggt
     2221 tcggcagtgc ctgagccgct taccgtattg agcgttatgc tgggctgttc aggattggga
     2281 tattacctca aaaaacgtct ttctaatatt ttttaaattt ttaatttttt aaataattta
     2341 aaatttttaa atattatcct tcacaattct acggttttag agcatgttaa atttgaaatt
     2401 gcgccttgtt gcgtcgttga agacgaggct ggcaaggaag gcgaagcagg caaccttata
     2461 tggtttccga tgcagcctga cgcagccagc cgacgccgca aacagcaaca aggcgcaaag
     2521 tcaagtttaa catgctctaa gagtttgcct taaaaaaacg acggtttcgt ctttgcagat
     2581 ttgcagatga tgccggaata tctgtgatta tttttcagaa ttgccatata aaattttcgg
     2641 cgatgataac gctataaaag atggggattt ttttcatcgc cgccattaga gcattcttta
     2701 gccgccgatg tcagcatcca cgttttaacg atgtcggcct ctggccgtgc ctgtactcac
     2761 atttttgccc gccgtcttgt ttgaaaaaca tactaagttg atatcgccga ctctgttttg
     2821 ctctgattat atgatattac aagacaggct attggagata taaaatagca gcgggacgga
     2881 tgcggaaaaa agcacccgcc cgccgctgtg gtgggccttg ggggcctcgt gggatttcta
     2941 tttctacatc attaaagatt ggaaatcaat ataaaactta agtttactta ataattcttt
     3001 tgttgactta attcgtgtca agtaatgaaa tcagggtatg gtcaggaaaa taaatacaat
     3061 tccttttggt gcggaaatac cgccggattt ggccgaaaag tttgacgaag tcatcgacga
     3121 atccggctgg actaaaaaac gagcgcttgc cgctgccgtc cgcgcgttct gcaagctgga
     3181 cgaatccacc cgcgccgacc tctacaaaaa agcatacgac aagctgaaat aatctggtag
     3241 ttttattgca cgcaaaattg tcaggttgcg tctctaagag tgttcatatc tgtaaaagaa
     3301 tttgtgagcc gattgtatga ttatttcatt ccgcgatatt cgaaaaaact caatcgcgat
     3361 tgccgccgtt atccgtcgat gtcggcagcc aagttttaat gacgttggcc atatggccgt
     3421 gc
//
LOCUS       DAONFI010000258         3402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_34447_length_3402_cov_3.812967, whole genome shotgun sequence.
ACCESSION   DAONFI010000258 DAONFI010000000
VERSION     DAONFI010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34447_length_3402_cov_3.812967"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLK08_08175"
     CDS             <1..350
                     /locus_tag="PLK08_08175"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53515.1"
                     /translation="YNSLSQDETPTGLWAIFDANSDGIGSVESGVWTLGDDDVLYAIG
                     ATTTGLNNIALVGDLPAYDSAYIYSYNSEQPDSYVNLPLPGINLVTGIPEPATLVLLG
                     TGLVASILARRKK"
     gene            694..1404
                     /locus_tag="PLK08_08180"
     CDS             694..1404
                     /locus_tag="PLK08_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53516.1"
                     /translation="MCLVACIVLGVGCLANIAAAETVSDFSDFSGNDSESDTYVTLTT
                     DRVNSDGLLVNGNLPESYNYKIGFTINGSDFIGYGNNANTQENGGEDNSTSWVSAYYR
                     NNSEKFFAGSSPTGLLVVLDAANDGIGTFDTNDNGTPDYEDDDTVHWTLGDDDVLYTA
                     GDVADILGSITYTGVIGRVEIGGVLDGIENLPAYSTSGSMVLPSINLITGVPEPATLA
                     LLGTGLTLAIFVRRRKSL"
     gene            1630..2541
                     /locus_tag="PLK08_08185"
     CDS             1630..2541
                     /locus_tag="PLK08_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS53517.1"
                     /translation="MNEQNDKKSQAARVVGDHRRQWRSCRFVYPVVSRRAGGLSVGIN
                     LNLDKRCNYSCMYCQVNRRIHGEKNDIDINRLHDELVETLNAFKTGEIWNEGNFVHTP
                     AEYRRLNDIAFSGDGEPTHITNFDQAVIAAVKALEDVGLKGCAKLVVITNATNLMARQ
                     VRNALSVLDANRGEFWCKLDAGTEHYFRTVNRPVPGLTLEKICENILSIAVGRPVVIQ
                     TLWFKIDGKSPSDMELEAYAGRLRWLVGCGGQIDHVQLHTIARPPSAANASAMPDAEL
                     DEIAKKIIALVPGVKFHVYYGQDVKPQ"
     gene            2557..3309
                     /locus_tag="PLK08_08190"
     CDS             2557..3309
                     /locus_tag="PLK08_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPS53518.1"
                     /translation="MEMETRAGGPGDWFWPHTQVHFQFNDKSCIVIVPENVADSRPWV
                     WRARFLGAFPYVDQDLLDRGYHIAFVNTADMFGAPAAMEIWDGFYELVRREFRLAPKM
                     TLEGFCRGGLFIYNWAARHPEKVNCIYADAPVCDIRSWPGGKGKGICDVLCWQKCLEM
                     YGLTEETVKNFHKNPIDLLEPIVAAGIPILHVAGDADDVVPIDENTYALAQRCRELGG
                     NINLIIKHGVKHHPHSLEQPDEIVKFIVENTK"
BASE COUNT          864 a          733 c          920 g          885 t
ORIGIN      
        1 actataacag tttgagtcaa gatgagactc ctacaggcct gtgggcaatt tttgatgcga
       61 atagcgacgg tattggaagc gtcgaaagtg gcgtttggac attgggggac gatgacgtac
      121 tttacgccat cggggctacc actaccgggt tgaacaatat tgcacttgtg ggcgatttgc
      181 ctgcttatga ttcggcgtat atttattctt ataattctga acagcccgat agttatgtaa
      241 atttaccctt gccagggata aatctggtta ctggcattcc ggaacctgcg acgcttgttt
      301 tgctcgggac tggtttggtt gcgtcgatat tggccagacg caaaaaatag ccggcaatcc
      361 tttggcagta ggaaagagat tagttttgac aaaaactctt tccggctgct ttttaactta
      421 ctccgaagtg accgattgac gatatttacc tttacgccta ttaatctgga aaccgttaaa
      481 tcggtcagtt tgaatacctt gtcgtgaaag ttatgttcca aacaatgttt atcttggaaa
      541 ggatttttca aaatgtccgt cggtcgttgt atttccacgt tcaggaaaat gcctcttaaa
      601 aataattccg acataatcct ggctgttttt gaatcaaaat ttcaatcaaa aataatggct
      661 gttgcgactg gagtaaaatt aacgaatatc gccatgtgct tagttgcctg tattgtcttg
      721 ggcgtcggct gtctggccaa tatcgcagcc gccgaaaccg tttctgattt cagtgacttt
      781 agtggtaacg attctgaaag tgatacttat gtaacgttga cgaccgacag ggttaattcg
      841 gacggcttgc tggttaacgg caatctgcct gagagctata attacaagat aggtttcact
      901 atcaatggca gtgattttat tggatacggc aacaatgcga atactcagga gaacggcgga
      961 gaggataatt ccacgagttg ggtaagcgct tattatcgca ataattcaga aaaatttttc
     1021 gcaggcagtt cgccgaccgg tttgttggtg gttcttgatg cggccaatga cggcataggg
     1081 acatttgata ccaacgacaa cggcacacct gactatgagg atgacgatac agtgcattgg
     1141 acgttgggag acgacgacgt actttatact gctggtgatg tagctgatat attagggagc
     1201 attacatata cgggggttat cggacgagtg gaaatcggcg gagtgcttga cgggatagaa
     1261 aatctgccgg cgtattccac ttcgggaagc atggttttgc cgtcaataaa tctgattacc
     1321 ggcgttcccg aacctgcgac gctggcgttg ctcggcacgg gtttaacgct ggcgattttt
     1381 gtcagacgca ggaaatctct gtaattgcct gaaaagcagg ttgcctgttt caacgttctt
     1441 gtcttaaaac ctttcgctga tgctctaaaa attgtgaacc acttttttga actgaatatg
     1501 aattttttta aaaggtgcgt catcgcagga ttgtgtgatg gccgcctttt ttgttccgat
     1561 tctggtataa gttgccatag ctgcggattg tgctggaaat ttgcgagtga tgggtgttag
     1621 aatgtggaaa tgaacgagca gaatgacaaa aaatcacagg ctgccagggt cgtcggcgat
     1681 catcgccgtc aatggcgtag ttgcagattc gtctatccgg tggtatcccg cagagcaggg
     1741 gggctgtccg tcggcataaa tctcaacctg gacaagagat gcaattattc gtgcatgtac
     1801 tgccaggtaa atcgtcgcat tcatggagaa aaaaacgata tcgacataaa tcgccttcac
     1861 gatgaacttg tggagacgct taatgccttc aagaccggcg aaatatggaa cgagggcaat
     1921 tttgtgcata cgccggctga gtatcggcgg ctgaacgata tcgccttcag tggcgacggc
     1981 gagccgacgc atataaccaa tttcgaccag gcggtcattg ccgcggtcaa ggctcttgaa
     2041 gacgtcggcc ttaagggctg tgcaaagctg gttgtcatta cgaatgcgac gaatctgatg
     2101 gctcggcagg tgcgcaacgc tttgagtgtg ctcgacgcca atcgcggcga attctggtgc
     2161 aagctcgacg caggcactga gcattatttc cggacggtca acaggcctgt gccgggtctg
     2221 acgcttgaaa aaatctgcga aaatattctt tcgattgccg ttggccggcc ggtggtgatt
     2281 cagacattgt ggttcaaaat tgacgggaaa agtccgtcgg atatggaatt ggaggcgtat
     2341 gccggcagac ttcgatggct tgtcggctgc ggcggccaga tagatcatgt ccagttgcac
     2401 accatagccc gtccgccgtc agcggcgaac gcatcagcca tgccggatgc cgagctcgac
     2461 gaaattgcaa aaaaaataat cgcacttgtt ccgggcgtga aatttcatgt atattacgga
     2521 caggatgtca agcctcagtg agatgcaagg tgtattatgg aaatggaaac tcgtgccggc
     2581 gggccgggag attggttctg gccgcatacc caggtacatt ttcagtttaa cgataaaagt
     2641 tgtattgtaa tagtgccgga aaacgttgct gacagccggc cgtgggtctg gcgagcaaga
     2701 ttcctgggtg cgtttccgta tgtcgaccag gatttgctgg acaggggata tcatatcgcg
     2761 tttgtgaata ctgccgacat gtttggtgcg ccggcggcta tggagatatg ggacggcttt
     2821 tacgaacttg tgaggcgcga attcaggctt gcgccgaaaa tgacgctgga gggcttctgc
     2881 cgcggcggtc tgtttatcta taactgggct gcgcggcatc cggaaaaggt gaactgcatt
     2941 tatgccgatg cgccggtgtg cgatatacgc agttggccgg gcgggaaggg caaaggcata
     3001 tgcgacgtat tgtgctggca gaaatgcctt gaaatgtacg gcttgacgga agaaaccgtc
     3061 aaaaatttcc acaaaaaccc catcgacctg ctcgaaccta tagtagccgc cggaataccg
     3121 attcttcatg tcgccggcga tgccgacgat gtcgtgccga ttgatgaaaa cacatatgca
     3181 ctagcccaaa gatgtcgcga actgggcggc aatatcaatc tgataatcaa gcacggcgtg
     3241 aaacatcacc cgcacagcct cgaacagccc gacgaaatcg taaaattcat cgtcgagaat
     3301 acaaaataga acggctctga attttatcgc catgccgtag tctttacgta gaacctatcc
     3361 caaaaacatg ggcgtcgccg aaatcgagca attttgcggg ct
//
LOCUS       DAONFI010000259         3396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_34535_length_3396_cov_3.583059, whole genome shotgun sequence.
ACCESSION   DAONFI010000259 DAONFI010000000
VERSION     DAONFI010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34535_length_3396_cov_3.583059"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLK08_08195"
     CDS             complement(<1..552)
                     /locus_tag="PLK08_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HPS53519.1"
                     /translation="MKRFFKRIIPGTARSGLLLIVVSCIIAGSLYFARSTVATPQAGE
                     EVAIVPVKNIKIVTKQPSRLEEIRRLAETDHIGLLKMALAEYDKKNIKSYTCRFIKQE
                     KLRGRLHEEQHVDVKFLAEPFCVAMTWVKNAPKADAIVYVEGKFPNSKGRCQMVVRPT
                     SGFLQWLVGGSVLRLPDGDDVMQN"
     gene            complement(824..1579)
                     /locus_tag="PLK08_08200"
     CDS             complement(824..1579)
                     /locus_tag="PLK08_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPS53520.1"
                     /translation="MQLNLVQIDNAWENPQANFDNITALLERKPPAANSLVFLSEMFA
                     TGFTDNSSLGTAIENDVSAFLASIARRWECYVSGGMVRGGKNCCVIFSPAGNLLHEYA
                     KQKLFTLSGENRHYAAGHNCSVTDICGVKVATLVCYDLRFPELFRQAIRLGAEMFIVP
                     ASWPESRQTHWRTLLQARAIENQACVVGISRIGCDPHTKHIGGSCIFDAEGRLILDAG
                     GEQGVFSCEFDPQEIRQWRRKFPALSDSGLIGL"
     gene            1699..2268
                     /locus_tag="PLK08_08205"
     CDS             1699..2268
                     /locus_tag="PLK08_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPS53521.1"
                     /translation="MQSSELDNFVTLAQQGRPDGVEWIMRQYSSRLYGYFLRSTGCMH
                     EAEDLMGELWVRLLRTLGSYSHTGRFDQWLFRIAANLVRDRIRRKVARGTIMSLDSGS
                     EGATLDHVDIDQPEPIEVLKSVEARHAVQAALAKLDEKTREMILMRHFGQMSFAELAK
                     EFNCPIGTVLARVHRGLQKLRQMLEGYEQ"
     gene            2265..2783
                     /locus_tag="PLK08_08210"
     CDS             2265..2783
                     /locus_tag="PLK08_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53522.1"
                     /translation="MNYSEKNYENVAHYMDGENVALNDIEQQLLAAVGDDIRATATVL
                     DASVDSEVMERVLRRHRNRKSPWRIYAAVAAAVLIVATAGWMFFGVNMDDAGKSPSTP
                     TSASVAVVNTTTNEVVETLAAVQSIMDAQQELDIMAMLVQNDDNTASTTTTTTSDNDI
                     WDMNAWANDLAG"
     gene            complement(2822..>3396)
                     /gene="rsgA"
                     /locus_tag="PLK08_08215"
     CDS             complement(2822..>3396)
                     /gene="rsgA"
                     /locus_tag="PLK08_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPS53523.1"
                     /translation="RFLIAAQRGGMQPILVINKTDIGRLADDAFASIRELQNTQIAYT
                     SALTGSGLDELKKILAGRRCVLAGASGVGKSTLVNSLVPQANAPTRDVRAKDLRGRHT
                     TSQAKIYSLDCGGILVDTPGIRELGLDMTADELCWYFPEIEEFAAGCKFSDCTHTHEP
                     GCAVVEAVNDGRIPHCRYETYLRILDTMTR"
BASE COUNT          788 a          849 c          904 g          855 t
ORIGIN      
        1 gttctgcatg acgtcatcgc cgtcgggcag gcgaagcacc gagccgccta ccagccactg
       61 aaggaaaccg ctcgtcggcc gaacgaccat ctggcaacgg cctttggagt tggggaattt
      121 gccttcgaca tatacgatgg cgtcggcttt gggggcgttc tttacccacg tcattgccac
      181 gcagaacggc tcggcgagga atttaacgtc gacatgctgt tcctcgtgaa gcctgccgcg
      241 gagtttttcc tgctttataa agcggcaggt atacgacttg atattctttt tatcgtattc
      301 cgccagcgcc atctttagca ggcctatgtg gtctgtttcg gcaagacgcc gaatctcttc
      361 gagcctgctg ggctgttttg ttacgatttt gatatttttt accggtacta tcgcaacttc
      421 ttcgccagcc tgtggagttg cgaccgtgct tcgggcaaaa taaaggctgc ccgcgatgat
      481 gcaacttact acgataagaa gtaatccgct tctcgccgta cccggtatga ttcgcttaaa
      541 aaaccttttc atataacacc tcattgtaaa cagcctgcca gtacattgca gcgcacgcaa
      601 gcatgtccaa caataatcgt cacattaata tctatcggcg ttttgggtca gccgcctgaa
      661 gatattatta acttggcatt atacccaatt caaagagccg gcgtttaaga tttttcaact
      721 tttttggtga agtttcgatt aaaacttttt tgacaaaaat gcctgttttt aatgtaacac
      781 attgttattg aaattgttat gaaaaatacc gtctttgcgg cgattatagc ccaatcagcc
      841 ctgaatccga caatgcaggg aattttctgc gccattgccg tatttcctgc gggtcgaatt
      901 cgcatgaaaa tacgccctgt tcgccgccgg cgtccagtat cagacggcct tccgcgtcga
      961 aaatgcaact gccgccgata tgcttcgtat gcgggtcaca accgattctg cttattccta
     1021 cgacgcatgc ctgattttcg attgcccttg cctgcagcag ggttcgccag tgagtctgcc
     1081 ggctttcggg ccacgaggca ggtacgatga acatttccgc ccccagccga atcgcctgcc
     1141 tgaaaagttc cggaaatcgc aaatcgtagc agaccagcgt cgctactttt acgccgcata
     1201 tgtccgtaac ggaacagtta tggccggcgg cgtaatgtcg gttttctccg ctgagtgtaa
     1261 aaagtttctg ttttgcgtat tcgtgcagca gattgccggc tgggctgaaa atgacgcagc
     1321 agtttttccc gcctcgcacc attccgcctg aaacgtaaca ttcccagcgg cgggcgatgc
     1381 tggctaaaaa cgccgagacg tcattttcga tggctgtgcc caagctggaa ttgtcggtaa
     1441 agcccgttgc gaacatttcg gagagaaaga ccagggaatt tgcggctggc ggtttacgct
     1501 caagcagggc ggttatgttg tcaaagttag cctgcggatt ttcccatgcg ttgtcaatct
     1561 gtaccaggtt cagttgcact gttttctcct tgagaacaac ttttttatca aatcaaaaat
     1621 tcggaatttc gcataagttt gacctatttc gatgaataat gcaataattg tccttaaaaa
     1681 cagttatact ataaaagaat gcagagttcc gaacttgaca attttgtgac acttgcccag
     1741 cagggcaggc cggacggagt ggaatggata atgaggcaat attcatcccg tctgtacggt
     1801 tattttctgc gttcgacggg ctgtatgcat gaggctgaag acctcatggg cgagttgtgg
     1861 gtacggctgc ttcgtacgtt gggcagctat tcgcataccg gccggtttga ccagtggctg
     1921 ttcaggatag cagccaatct tgtgcgtgac cgcatacggc gaaaagtggc cagaggcaca
     1981 ataatgagcc tcgattccgg cagtgaaggt gcgacgctcg accacgtcga tatcgaccag
     2041 cctgagccga tagaggttct caaaagcgtc gaggcacgcc atgctgtgca ggctgctctt
     2101 gcaaaacttg acgaaaagac ccgcgaaatg atactgatgc gacatttcgg gcagatgagt
     2161 ttcgctgaac tggcaaaaga gttcaactgt cccataggga ccgtattggc ccgcgtgcac
     2221 agaggccttc aaaaactccg gcagatgctg gaaggatacg aacagtgaac tattcagaaa
     2281 aaaattatga aaacgtagcc cactatatgg acggcgaaaa tgtggcattg aatgatatcg
     2341 aacagcaact tcttgccgca gtcggcgatg acattcgggc gactgccacc gtgctggatg
     2401 caagcgtaga tagcgaggtc atggagcggg ttctgcggcg gcacaggaac aggaaatcgc
     2461 cgtggcgtat atatgccgct gtcgcggcgg cggtgttgat agtggcgact gccggctgga
     2521 tgttttttgg ggtaaatatg gacgatgccg gcaaatcgcc gtcgactccg acatctgcgt
     2581 cggtggcggt ggtaaatact acgacgaatg aagtagtcga gacgctcgcc gccgtgcagt
     2641 ccatcatgga cgcccagcag gaattggaca ttatggcgat gctggtgcag aatgacgaca
     2701 ataccgcctc cactacgact acgacgacat ctgataatga tatctgggac atgaacgcct
     2761 gggcaaatga cctggcggga taatcaatcg ggcagctttg tgaagcgccc tggtttattt
     2821 tttatcgcgt catggtatca agtatgcgca gataagtttc atagcggcag tgtgggattc
     2881 tgccgtcatt taccgcttcg acgacagcac agccgggttc gtgcgtatgt gtacagtcgc
     2941 tgaatttgca gccggcggcg aattcctcta tttccgggaa ataccagcat aattcgtcgg
     3001 cggtcatgtc cagcccaagt tcccttatac cgggcgtgtc taccagaatg ccgccgcagt
     3061 cgagagaata aattttcgcc tgactggtcg tatgacggcc tcgcaggtct ttggcccgca
     3121 cgtcgcgagt cggagcgtta gcctgcggca cgagagaatt caccagcgta cttttgccga
     3181 cgcccgaagc gccggctaat acgcatcgtc ggccagccag gatttttttc agttcatcca
     3241 gtccgctgcc tgtcagggcc gatgtatacg ctatctgggt attctgcaat tcgcgaatgg
     3301 aggcgaatgc gtcatcggca agccggccga tgtccgtctt gttgattacg agaatgggct
     3361 gcatgcctcc gcgctgtgcg gcgatgagaa accgtt
//
LOCUS       DAONFI010000260         3389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_34643_length_3389_cov_3.819736, whole genome shotgun sequence.
ACCESSION   DAONFI010000260 DAONFI010000000
VERSION     DAONFI010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34643_length_3389_cov_3.819736"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..386
                     /locus_tag="PLK08_08220"
     CDS             213..386
                     /locus_tag="PLK08_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53524.1"
                     /translation="MKRRIKNVEKISGQMTVEWALLIAAFGLPMIAVFRLMLTALTGF
                     YQMLTCVISLPLP"
     gene            484..1095
                     /locus_tag="PLK08_08225"
     CDS             484..1095
                     /locus_tag="PLK08_08225"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPS53525.1"
                     /translation="MHIPVTLQYWPFMLAGVLLAVSAWSDYRRHVIPDAFTLPVLLIA
                     LAGHLACSFLVAKNPDNNNIFSAVGVMGFRDSLYGLLLGGVPMTFVWRAGGVGGGDVK
                     LLAAVGATLGWRWTLDIMFVAFILSAAWAIIIIIRKNIAARIFMNIWTFLAHAHSRKE
                     KNRSTANSPGIEFALAAAGGFIIAVVCNIANGMNGNFTMNFAM"
     gene            1256..2071
                     /locus_tag="PLK08_08230"
     CDS             1256..2071
                     /locus_tag="PLK08_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53526.1"
                     /translation="MSYGGGVFDGPVLRSALATPLFRFYLSIAAISLLVISVILGFAA
                     RKRRKPLPATAGTIIIEFTLVLPILLMLSLILLQSSLVLAGHLCVTYAGFCAARSAIV
                     QIPQNYTDEKTNYLNTSGDSQKMSHILDAACWAVMPVCDGSYAGSTADDRLTQGFETL
                     YSENGQTAPKWVSSYLAAKLAYAKSHTTVTVAVPEEDDYYSEHEDIKVDVTHDLYLSV
                     PYADVLLATLDSDHAVELSDDKRAIRVTTTCTLTNQGTSMEIPVESFPKTDDD"
     gene            2061..>3389
                     /locus_tag="PLK08_08235"
     CDS             2061..>3389
                     /locus_tag="PLK08_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HPS53527.1"
                     /translation="MMTDRTKIKIYPTLNNITHRRERRGAVLFIVLIAMTLLVGLVFF
                     VLNAGTQVNRRLELQDTADSTAMGGANWMARSMNVIAMDNVGMTRMIMMSAMLDSMPL
                     AAEMTIAEETGEKSLPLALTRFKTLGPAYTEMEQDNFFRRGLAEIYRQMNYEDNNTTD
                     LDLLKKIDDTFDQKDELALEDGYDVAPTTQWEYSGSVQYVIDDNDGGFATTGSWQRAE
                     LSQQYSTCCYTTTSSGATATWTKYLPKSGEYAVYARWPSSDSLNQGASYTINYSGGSQ
                     DVLRDQTRNGGKWVKLGEFSFSSDSPAQVTLTHTGTAASAADAIKFISSSNEKNRGYV
                     WRAAVAMDKLSQATVENLADRVSQNANLSGETNNASIAFCAPLKPALPCERFDFSKFE
                     SVLVDNVNYDRDTATVRKSDLVDYLYNSDDPAQAARYKTKNGVRDTVKGWG"
BASE COUNT          868 a          851 c          849 g          821 t
ORIGIN      
        1 gatactttca tctacggttt aaaaaccgta gaattacgaa ggacatatta aaataacaac
       61 atttcacaaa aatgcaacag gggtgacttt tcattggaaa acggtcagaa agtgaaataa
      121 aataattacg gtaagcgtta atcgctcaaa ttacacttac gtatgggact ttacgaaggg
      181 tgacatcaac aacgttaaga gtttttaaca aaatgaaacg tcgcataaaa aatgttgaga
      241 aaatttccgg gcagatgacg gtggaatggg cattgcttat cgccgctttc ggattgccga
      301 tgatagccgt atttcgtctc atgctgacgg cacttaccgg cttttatcag atgctcacat
      361 gcgttatttc tctgccattg ccgtaattgc aattattttc agccctgttt gaaactattt
      421 atacgtttta tgcgtttgat aacagagtaa attcaacccg accgatttga gcagaaataa
      481 gatatgcata ttcccgtgac gttacaatat tggcccttca tgctcgccgg cgtgctgctg
      541 gccgtttcgg catggagcga ttatcgccgc catgttattc ccgacgcatt taccctccct
      601 gttctgctca tcgcattagc cggccatctt gcgtgcagtt tcctggtagc aaaaaatccg
      661 gacaataata atatcttttc agccgtcggc gtgatgggtt ttcgtgacag cctttacggt
      721 ttgcttctcg gcggcgtgcc gatgacgttt gtctggcgtg ccggcggagt cggcggcggc
      781 gatgtaaaac tgctggcggc ggtcggcgcg acgctgggct ggcgatggac gctggatatc
      841 atgttcgtcg cgttcatact ttcagcagcc tgggctataa tcatcataat ccgaaaaaac
      901 atcgccgcca gaatattcat gaacatatgg acgttccttg cccatgcgca cagccgcaaa
      961 gaaaaaaatc gttcgacggc taactcgcct ggaatagaat ttgcgttggc agccgccggc
     1021 ggattcataa tagccgtcgt ctgcaacatc gccaacggca tgaacggcaa ttttacgatg
     1081 aatttcgcca tgtgatttgg ccttaaaagt attacaacgg tatttttcga ggaaatggaa
     1141 tatttttaat aactttaaat cttttaattt ttttaaaaat ttaaaaaaat ttaaaaaatt
     1201 tttaaaaaga acgctgatta ttgcggcggt gttatctgtc gcctgcattg caatcgtgtc
     1261 ttacggcggc ggcgtttttg acggccccgt tttgcgcagt gcattagcaa cgccgttatt
     1321 ccggttttat ctttccatcg ccgcaatcag cctgctggtt atctcggtta tactcggttt
     1381 cgcggcgaga aaacgcagaa aaccccttcc cgccaccgcc ggtacaatca taattgaatt
     1441 tacgctcgtt cttccgatac ttttgatgct ttccctgatt ctgcttcaga gttcgctggt
     1501 gttggccggc catctttgtg tgacatacgc aggcttctgc gccgcccgca gtgcgatagt
     1561 acagataccg cagaattata ctgacgaaaa gaccaattat ctgaacactt ccggcgattc
     1621 gcaaaaaatg tcacatattc ttgacgccgc ctgttgggcc gtcatgcctg tttgcgacgg
     1681 cagttacgcc ggcagcactg ccgatgaccg gctgactcag ggctttgaaa cgctttattc
     1741 cgaaaacggc caaaccgccc ccaaatgggt cagcagttat ctggccgcaa aactcgccta
     1801 cgccaaaagt catactacgg ttacggtggc tgtaccggag gaagacgatt attacagcga
     1861 gcacgaagac atcaaggttg acgtcacgca cgacctttac ctgtctgtgc cgtatgccga
     1921 cgtactgctc gcaacattgg actccgacca tgccgttgaa ctttcggacg acaaacgggc
     1981 tattcgagtt acgaccacat gtacgctgac gaaccagggc acaagcatgg aaattccggt
     2041 agaaagtttc ccgaagacag atgatgactg accgtacaaa aataaaaatt tatccgacac
     2101 tcaataatat cactcaccgg cgcgagaggc gaggcgcggt gctgtttata gtattgattg
     2161 ccatgacgct gctggtgggg ctggtgtttt tcgtactcaa tgccggcact caggtcaaca
     2221 ggcgtctcga gcttcaggac accgccgatt cgacggctat gggcggtgcg aactggatgg
     2281 ctcgcagcat gaacgtaatt gcgatggaca acgtgggcat gacccgcatg ataatgatgt
     2341 cagccatgct ggactccatg ccgcttgccg cggaaatgac catcgccgag gaaacgggcg
     2401 agaaatccct gccgctggcg ctgacaagat tcaaaacgct cgggccggct tatacggaaa
     2461 tggaacaaga caatttcttc cgcagggggt tggccgaaat ttacaggcag atgaattacg
     2521 aggacaacaa taccaccgac ctcgatctgc tcaaaaagat agacgatact tttgaccaga
     2581 aggacgaatt ggcgttggag gacggttacg acgttgcgcc tactacgcag tgggaatatt
     2641 caggttcggt tcaatacgtc atagacgaca acgacggcgg ctttgccacc acgggttcat
     2701 ggcagcgggc ggagctttcg cagcaatatt caacgtgctg ttatactacg acgtcttccg
     2761 gcgcgacggc gacctggacc aaatatctgc caaagagcgg ggaatatgcg gtatacgccc
     2821 gctggccgtc cagcgacagc ctgaaccagg gtgcatccta cacgataaac tattccggcg
     2881 gttcgcaaga cgtcctgcgc gaccagacaa gaaacggcgg caaatgggtc aagcttggcg
     2941 aattttcatt cagttccgat tcgccggcac aggtcaccct gacgcacacc ggcacagccg
     3001 ccagcgcagc cgacgcaata aaattcatca gttcgtcgaa tgaaaaaaat cgcggttatg
     3061 tctggcgcgc agcggtggcg atggataaat tatcgcaggc taccgtcgaa aacctggctg
     3121 accgcgtctc gcaaaatgcc aacctttccg gcgaaacaaa taatgcgtcg atagcattct
     3181 gcgccccact caagccggct ctgccgtgcg agcggttcga tttcagcaag ttcgaaagcg
     3241 tactcgttga caacgttaat tacgatcgtg atacggcgac cgttcgcaag agtgaccttg
     3301 tggattatct gtacaattcc gacgaccccg cgcaggcggc tcgctacaag accaaaaacg
     3361 gcgtgaggga cacagtcaaa ggctggggg
//
LOCUS       DAONFI010000261         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_34661_length_3388_cov_3.747075, whole genome shotgun sequence.
ACCESSION   DAONFI010000261 DAONFI010000000
VERSION     DAONFI010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34661_length_3388_cov_3.747075"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(557..628)
                     /locus_tag="PLK08_08240"
     tRNA            complement(557..628)
                     /locus_tag="PLK08_08240"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Asn,seq:gtt)
     gene            complement(813..1622)
                     /gene="proC"
                     /locus_tag="PLK08_08245"
     CDS             complement(813..1622)
                     /gene="proC"
                     /locus_tag="PLK08_08245"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPS53528.1"
                     /translation="MNDYKLGVIGGGNMGSAIVGGAVRSGFLAGGEILISEPLDAKRV
                     QLSANLGVTCVDNIPATAGCEKILLAVKPQVMPQIIPIVAANLADDTLIISIAAGLNT
                     KFFDAALNGRGRIVRVMPNTPMLVGEGASAVCSGPRATAADVEWTKKLLTAGGGTVVE
                     VEEKQMDAVTAVSGSGPAYFFYLLEAMIDAGVAEGLSRETATLLAVQTCKGSAVLQNV
                     TGESPEELRRKVTSPGGTTQQAIDVFDAEKTKETMIRAVRAAADKSRLLGR"
     gene            complement(1787..2347)
                     /locus_tag="PLK08_08250"
     CDS             complement(1787..2347)
                     /locus_tag="PLK08_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPS53529.1"
                     /translation="MPLSANSDNDAVKIAGKELIQRIERVMDDIDWSSFLKLANMIPA
                     GKRTFVTGAGRSGLVARMFGMRLMHAGLTAYIPGETITPAAGVGDIMVAISCTGQTGY
                     TSYLVKRAKEFGAKVVIITSKPDSPLASHADHLVTIPAKAEDIVLRAAVFEHVTSLCL
                     DAVFNVLSERFKIDIAAFQRRHANLE"
     gene            complement(2331..>3388)
                     /locus_tag="PLK08_08255"
     CDS             complement(2331..>3388)
                     /locus_tag="PLK08_08255"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HPS53530.1"
                     /translation="LGCADEATIRECVEAGRHYGLQVAVDLLGVADPVAFAKNAKNLG
                     VAWLDVHCAIDQQMKGMTPQNIVKEIRGATDLILAVAGGINSETAPATANAGADVVIV
                     GGAITKAIDPAAAAADIKKALATGKAVASELFKRSSASDIRKTLEKVRTSNISDGSHR
                     QPCLAGIRPLNPGQFACGPAVTVRTMPGDWAKPLEAIDVAKEGDFLVIDAAGQPPAVW
                     GELASEGAKNRKIAGLVIHGAARDTAEIRKLGFKVWSSLVTSHAGDPHGLGEINVPII
                     IASQRICPGDWIVADDDGVIVLPADKAVEMANRAADVLEAENRIRTEIQKKNSTLAKV
                     VNLRRWEKQGVGNATIG"
BASE COUNT          710 a          896 c          929 g          853 t
ORIGIN      
        1 ccaagaccgc aaagcagggc aagccgacac gggttattcc gctgtttcct gaactgtacc
       61 cgtatctctt ggattcgttt acaaaggctg aggatggtca ggaatacgtt gtcgggcatt
      121 attcgagtcg cacgctgtcc aaggcggcgt tgaggacaca ggctgtccgt ataatcaggc
      181 gtgcaggcct tgagccgtgg cccaagttgt tcaccaactg ccggtcaagc tgcataatcg
      241 acctttcgca taaatttccg attcatgttg ctaccgcgtg gatgggacat tctccaaaaa
      301 ctgcaatgac gcattatctt gccgttctgg aaagtgattt tgagaaagcg ttgaaggtcg
      361 agaaacacga cgcaaattcc gacgcattca ctggaaaaac gacttctgga atgacgcgaa
      421 atccgacgca gaacctagcc gcaatgagtg gaattgaaaa gaaagaaagt tattttggcc
      481 ttgaaaacat tgggggaaat gcgagtcatt gcgatttggt gaatgctgtt gcgagtggat
      541 gcgaacaaga accaaactcc cccggaagga ctcgaacctt caaccctgcg gttaacagcc
      601 gcatgctcta ccattgagct acaggggaat ctgcctgctc agcaagaagt gcatacttta
      661 ttgaatctgg tgtttttgtc aagtggattt tttcaaaaag tgcagcagag gcgtgatttg
      721 cataaattac tcaaaattta ttcagcggaa tcttttcaat atactggctt acgaaacatg
      781 ataaattgct aaaaatggtc gcttacgcaa aattaccttc cgagcaggcg gcttttatcc
      841 gccgccgcgc ggactgcccg tatcatggtc tctttagttt tttcggcatc aaacacatcg
      901 atagcctgct gcgtcgtacc gcctggcgac gttaccttgc gacgaagttc ttctggcgat
      961 tcgccggtga cgttttgcag gacggctgag cctttgcagg tctgtaccgc aagcagggtg
     1021 gctgtctcac gcgagaggcc ttccgcgacg ccggcgtcta tcatggcttc gagcaggtag
     1081 aagaagtacg ccggcccact gccggatacg gcggttacgg cgtccatctg cttttcttcc
     1141 acctcgacta ctgtgccgcc gccggcggta aggagttttt ttgtccattc gacatctgcc
     1201 gccgtcgcac gcgggccgga acagaccgcc gatgctccct cgccgacaag catgggtgtg
     1261 ttgggcatta cgcgaactat gcggccacgg ccgttcagtg cagcatcaaa aaatttcgta
     1321 ttcaaaccgg ctgctatgga aataattagc gtgtcatcgg caaggttggc ggcgacgatt
     1381 gggattatct gcggcatgac ctgcggcttg acggctaaca ggattttttc gcaaccagcc
     1441 gtcgccggaa tattgtctac gcacgttacg ccgagattgg ctgaaagctg aacgcgtttt
     1501 gcgtccagcg gctcggatat gagtatttcg ccgccagcca ggaatccgct gcggactgct
     1561 ccgccgacta tggctgaacc catgtttccg ccgcctatta cgccaagttt gtaatcattc
     1621 atttttaaaa cccgaataaa aaatcaaaga taaagcccaa aaatttccgc aatggccgac
     1681 aggctatatc ataccgtcaa ccgatttata atacggtatg ccctgagacg cacagtcgcc
     1741 tgcatcctgt ttgtacctga aatcgccgga ttttgaaatt caaaatctat tccaggtttg
     1801 catgtctgcg ctggaatgcg gcgatgtcta ttttaaaccg ttcggacagg acgttgaata
     1861 cggcatccag gcaaaggctc gtcacatgct cgaaaacggc ggcacgcagc acgatatctt
     1921 cagctttcgc gggtattgtc accagatgat ctgcgtggct ggccagcggc gagtctggct
     1981 ttgacgttat tatgacgacc tttgcgccga attccttggc tcgcttgacc aggtagctgg
     2041 tatatcctgt ctggccggtg cagcttattg ccaccattat gtcgccgacg cctgctgctg
     2101 gcgtgattgt ttcgccaggg atatatgccg tcaatcctgc atgcatgagt cgcatgccga
     2161 acattctggc taccaggccg ctgcggccgg cacctgttac gaaggtacgc ttgccggctg
     2221 gtatcatgtt ggcgagtttt agaaaactgc tccagtcaat gtcatccatc acccgctcta
     2281 tacgctggat gagttccttg ccggcgattt ttactgcgtc gttatccgaa ttagccgata
     2341 gtggcatttc cgactccctg cttttcccat cgccggagat tgactacctt agccagcgta
     2401 gaattctttt tttgaatttc cgttctgatt cgattttcag cttcgagcac gtcggctgcg
     2461 cggttggcca tttcgacggc tttatccgcc ggcagtacga ttacgccgtc atcgtcggct
     2521 actatccagt cgccggggca gatacgttgg ctggcaatga ttatgggtac attgatttcg
     2581 cccagcccgt gcggatctcc tgcgtgcgac gtcaccagtg acgaccagac cttgaaaccg
     2641 agcttgcgta tttcggcggt atcgcgggct gctccgtgga ttacgaggcc ggcgattttc
     2701 cggttttttg cgccttcgct ggcaagttcg ccccagacag ccggcggctg gccggcggcg
     2761 tctatcacca gaaagtcgcc ttcttttgcg acgtcgatgg cttctagggg tttggcccag
     2821 tcgccgggca tggtccgaac ggtgacggct ggcccgcatg cgaactggcc gggattcagc
     2881 ggccgtatcc ctgccagaca gggctgacga tgagagccgt cggaaatgtt gctggtgcga
     2941 actttttcaa gcgttttgcg gatgtcagac gcagaggaac gtttaaaaag ctcgctggcg
     3001 acggctttgc cggtggcgag tgccttttta atgtcagcgg cagctgcggc tgggtcgata
     3061 gcttttgtaa ttgctccgcc gactattacg acatctgcgc cggcgttggc ggtggcgggg
     3121 gcggtttcgg aattgattcc gccggcgacg gctaatatga ggtcggtcgc tccgcgtatt
     3181 tccttgacga tgttttgcgg agtcatgcct ttcatctgct ggtcgatggc acagtgcacg
     3241 tccagccatg ccacgccgag gtttttggcg tttttggcaa atgcgacagg gtcggcgacg
     3301 cccagcaggt cgaccgccac ctgcaagccg taatgtcggc cggcttcgac gcattcgcga
     3361 atggtggctt catcggcgca acccagta
//
LOCUS       DAONFI010000262         3357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35119_length_3357_cov_3.554210, whole genome shotgun sequence.
ACCESSION   DAONFI010000262 DAONFI010000000
VERSION     DAONFI010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35119_length_3357_cov_3.554210"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1097
                     /locus_tag="PLK08_08260"
     CDS             105..1097
                     /locus_tag="PLK08_08260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HPS53531.1"
                     /translation="MNLENLVIIFARSGYNPAMFKTIITVTLNPAIDRAIEVSQLEIG
                     SHAQCRTVSRIAGGKGVNVSRVLADMGVASVATGFLGSENRSVFDSVFAQGLIEDSFV
                     DVNGLTRENLTLVNPAVGAEMHLRDEGLAVGKGDIDELASRLKCRINPQCVVIFCGSL
                     PPGVGCREMSKLLDISLAGGAMVAVDASGKALQAAGGKKLWLLKPNAIELSELVGREF
                     SDDAELLDAARSLTDDVENILLSCGGDGAYLVRAEGVLQCLPGMAVDVVNTVGCGDVL
                     LGAFVGSLYRGGDYRDAIMHAVACASASAANTQTAKFQQELADKIFNGLFIKKL"
     gene            1122..1838
                     /locus_tag="PLK08_08265"
     CDS             1122..1838
                     /locus_tag="PLK08_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPS53532.1"
                     /translation="MQRINGVIFDMDGVLCDSERFIRDAAMEMMRRDYSLSVKPDDFL
                     PFIGAGESRFISGVAEKYGVKLTLPRDKMRTYQIYLELIKGSLQPLDGVLEFIGECRR
                     RGVRLAVASSADMVKVTGNLTEIGLPMATFDAVITGDDVTRHKPDPECFLKAAEALRL
                     SPSACMVVEDANNGVKAAKAAGAHCLGLTTSFTPKNLKTSGADWTAPTLAEVPQEVMA
                     ILGGEKTNETLPSDPLCSYH"
     gene            1850..3145
                     /gene="hemL"
                     /locus_tag="PLK08_08270"
     CDS             1850..3145
                     /gene="hemL"
                     /locus_tag="PLK08_08270"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPS53533.1"
                     /translation="MKTSKSREAFERACKVLVGGVNSPVRAFAAVGGQPPLISRACGS
                     HIIDLDGNEYVDYVCSYGPLILGHAQQEVVTAIHKAVLRGTSFGATTEEEVELAELVI
                     SAIPRAEKIRFTSSGTEAVMTALRLARGVTGRRMVVKFRGCYHGHCDAMLVQAGSGAT
                     TTGQPSSAGVPVGATSDTLLADYNDLDSVRCLFEEYGSEIATVLVEPVAGNMGVVPPA
                     GGFLQGLREICDAGGAMLIFDEVMTGFRVAWGGAQELYGVQADITTLGKIIGGGMPVG
                     AVAGPSEIMDRLSPLGDVYQAGTLSGNGPAMAAGIATLTAIRNDADFYSRLDENAGLL
                     ERMLRTAAGDAAVSDRLTFNRVGSMMCVFFRPGEVKNYDQALECDKNAFAKFFGKMLG
                     EGVFLPPSQYEAWFVSSSHTQEDFDLTASAAKKAFEEIF"
BASE COUNT          726 a          802 c         1024 g          805 t
ORIGIN      
        1 cctgttattt tggaaacacg gggcatttta accggaaagg gcaggaaacg gtttgccgca
       61 tcggtttcca tatatataac gtctcagcta gccggaaagt tccgttgaat ttggaaaatt
      121 tggtcataat ttttgcccgc agcgggtata atccggccat gttcaaaacc atcatcaccg
      181 tgactttgaa tcctgcgatt gacagggcta tcgaggtttc gcagcttgaa atcggctcgc
      241 atgcgcaatg tcgcaccgtc agccgcatag cgggcggcaa aggcgtaaac gtctcgcgcg
      301 tgttggctga tatgggtgtt gcttccgtgg cgacggggtt tctcggcagt gagaatcgca
      361 gcgtatttga ctccgtcttc gcgcagggcc ttatagagga ttcgtttgtc gacgttaatg
      421 gccttactcg cgaaaatctt actctggtca atccggccgt cggtgccgag atgcatcttc
      481 gcgatgaagg ccttgccgtt ggaaagggtg acatcgacga actcgccagc cggctgaagt
      541 gtcggattaa tccgcaatgc gtggtgattt tctgcggctc actgccgcct ggcgttggct
      601 gtagagagat gtcgaaattg ttggatattt ctctggctgg cggggcgatg gtggctgtgg
      661 atgcgtcggg caaagcattg caggctgccg ggggcaaaaa attatggctg ctcaagccca
      721 acgccattga attatctgaa cttgtcgggc gggaattttc cgatgacgca gaactgctcg
      781 acgcagccag gtcgctgacg gatgatgttg agaatattct cttaagttgt ggcggagacg
      841 gggcttatct tgtgcgtgcc gaaggcgtgt tgcagtgtct gccgggcatg gcggtcgatg
      901 tggtgaatac ggttggctgt ggcgatgtgc tgctgggagc gtttgttggc agcttgtatc
      961 gcggcggcga ttatcgcgac gcaatcatgc atgcggtagc ctgcgccagc gcctctgccg
     1021 ccaataccca aaccgcgaaa tttcaacagg aactggcaga taaaattttt aatggtcttt
     1081 ttataaaaaa attataaaaa agttataaaa gaggttaaat aatgcagcgt attaatggcg
     1141 tgatttttga tatggacggc gttttgtgcg attcagaacg gttcatacgc gatgcggcga
     1201 tggagatgat gcggcgtgat tatagtctgt cggtcaagcc ggatgatttt ttgccgttta
     1261 tcggtgccgg cgaaagtcgc tttatcagcg gggttgcgga aaagtatggc gtaaaactga
     1321 cccttccgcg ggataaaatg cgaacttacc aaatatatct ggaactcatc aaaggctctt
     1381 tgcagccgct ggacggcgta cttgaattta tcggcgaatg ccgccgtcgc ggcgtcagac
     1441 tcgccgtggc cagttctgcg gatatggtta aagttacggg caatcttacg gagataggtt
     1501 tgccgatggc tacgttcgat gccgtcataa caggcgacga tgtgactcgt cacaagcctg
     1561 accctgaatg ttttctcaag gctgccgagg cgttgcgtct ttcgccgtct gcctgcatgg
     1621 tcgtggagga cgccaacaac ggcgtaaaag ccgccaaagc cgccggcgct cactgtcttg
     1681 gccttaccac gagctttacc ccaaaaaacc tcaaaaccag cggtgcagat tggaccgcgc
     1741 ctactttggc tgaagttccg caggaagtta tggctatcct gggcggcgaa aaaacgaatg
     1801 aaaccttgcc atccgacccg ctttgcagtt atcattaaca aaaatggaca tgaagaccag
     1861 caaatcccgt gaagcattcg aacgtgcctg caaagtgctg gtcggcggag ttaattcgcc
     1921 ggtcagggcg tttgccgccg taggcggcca gccgcccctg atatcgcgtg cctgcgggtc
     1981 gcacataatt gacctcgacg gcaatgaata tgtggattac gtgtgcagtt atgggccgtt
     2041 gattctcggc catgcgcagc aggaggtggt gacggctatt cacaaagccg ttctgcgcgg
     2101 cacgtctttc ggcgcgacca ccgaagaaga ggtcgaactt gccgaattgg tgatatcggc
     2161 tattccgcga gctgaaaaga taagattcac gtccagcggg acggaagcgg tgatgacggc
     2221 tcttcggtta gcccgcggcg ttacgggccg gcggatggtg gtgaagttca ggggctgtta
     2281 tcacggccat tgcgacgcca tgctcgtgca ggctggcagc ggtgcgacga ctaccggcca
     2341 gccgtccagc gcgggcgtgc cggtaggcgc gacgagcgat acgctgctgg cggattataa
     2401 cgaccttgat tccgttcgct gcctttttga agaatacggc agtgaaatag cgacggtact
     2461 cgtcgagccg gtagccggca atatgggcgt agtgccgccg gctggcggtt ttctgcaggg
     2521 cttgcgggaa atatgcgacg ccggcggagc gatgttgata ttcgacgaag tcatgacggg
     2581 tttccgcgtg gcgtggggcg gcgcacagga actttacggc gtacaggctg acataaccac
     2641 gctgggcaaa atcatcggcg gcggcatgcc ggttggtgcg gtggcggggc cgtcggaaat
     2701 aatggacagg ctttcgccgc tgggcgacgt ttaccaggct ggcacgcttt ccggcaatgg
     2761 gccggcgatg gcggctggca ttgcgacgtt gacagccata cgcaatgacg cggattttta
     2821 ctccagattg gacgaaaacg ccggcctgct tgagcgtatg ctcagaacag cagccggcga
     2881 tgcagccgtc tcggacaggc tgacgttcaa tagggtaggg tcgatgatgt gcgtgttctt
     2941 ccggcctggc gaggtgaaaa attacgacca ggctctcgaa tgcgacaaaa atgcgtttgc
     3001 aaagtttttc ggcaaaatgc tcggcgaagg tgtgttcctg ccgccgagcc agtatgaggc
     3061 gtggttcgtt tcatcctctc atacgcagga agatttcgac ctgaccgcca gtgcggcgaa
     3121 aaaggctttt gaagaaattt tttaaaatgt ctcccgtagt tcaacgatga tttttacaaa
     3181 agattgagga ggtttttatc atgccagcca ttcaaacatg cgaatttaac gccgcgtttc
     3241 gtagtctgca cagggctaac ggcattttat gcctgataat tctgctctgc ctggcggctc
     3301 agatagccgg tttctgtgcg gtggaattcg gcggagttct tgataagtgt acggtta
//
LOCUS       DAONFI010000263         3355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35161_length_3355_cov_3.976970, whole genome shotgun sequence.
ACCESSION   DAONFI010000263 DAONFI010000000
VERSION     DAONFI010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35161_length_3355_cov_3.976970"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1816)
                     /gene="rpoD"
                     /locus_tag="PLK08_08275"
     CDS             complement(149..1816)
                     /gene="rpoD"
                     /locus_tag="PLK08_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPS53534.1"
                     /translation="MSEILDISVKKLIAKGKERGYLTYEEMNDDLPDSAVTPERLDSL
                     LMTLDELGIELVDEADIADESDKFSDDSLEDDEEKSPEDQLSEELAMESRRIDDPVRM
                     YLTQMGEIPLLRRDQEISLAKKIEMTRMHFRRRVLESDYCIREALNILSQVHSGDLPF
                     DRTMKISSAENLSKETITDRMPTNLTTLEKVLVANNENWGAYKTSVTGRDVAEVKKQT
                     KILWRRRRRAAKLLEELSLRTSRIQPLMKKLNQFSDKMSNIEKEIEIAPRKNTPQEDV
                     DVMREELQGLIDLVHEEPDMLRKRVKSIRKAFNDYEQAKRELSGGNLRLVVSIAKKYR
                     NRGLSFLDIIQEGNTGLMRAVDKYEYRRGYKFSTYATWWIRQAITRAIADHARTIRIP
                     VHMIETMSKLRNISKKLIQSLGREPTIEEIAHEADMSVSEARRVMKISRHPISLDRPV
                     GESEDSYFGDFIEDETAESPLESAGSEMLKDRIEDVLKTLTYREREIIKLRYGIGDGY
                     TYTLEEVGRIFKVTRERVRQVEAKAIRKLQHPVRARKLEGFLDGKHL"
     gene            complement(2065..>3355)
                     /locus_tag="PLK08_08280"
     CDS             complement(2065..>3355)
                     /locus_tag="PLK08_08280"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPS53535.1"
                     /translation="LVKYTLEGRLVVRLKGGDCFIFGRGGEELDALEAAGCQYRVVPG
                     ITAAIAAGAYGGISLTDRRYASGVAFVTGHEDPAKQTSSLNYTALAGIDTVVFYMGVG
                     HLEPITTGLIAAGRAADTPAAVVRMAGRPSQKTLIGTLGDIFGKTRDAGVEPPAVLIV
                     GQAAAKARGWYEKLPLAGKTVLVTRPLKQSVPLAGMLRRSGAEVIETPTIEIAPPENF
                     SPLDAAIEHLASYDWLVFTSPNGVIFFMSRLAVAGLDARALAGVKIAAVGPATAGKLA
                     DFGLLADLTPKCNYTTAGLAADLKLRLTPGNTVLLARSDIAPPDLKNALEPAAAKVDE
                     VCAYCTRCPAELPPDAINALRQGHVDIITFTSSSTAENFVKLLKKAEPQAFLRIINNI
                     KKAAIGPVTAMKLQELGFPADVVAAECSVAGLVAALV"
BASE COUNT          759 a          929 c          817 g          850 t
ORIGIN      
        1 acgaacaaac ccactggcgc aaaaatatga actctcaatc tttcaatgct ctaacccgcc
       61 aaccatatgc tatagaaaaa tacagacaac aaaaaaaccc gccatatcat tgatatcgcg
      121 ggttttattt tatcctacta ttattgactt acagatgctt gccgtcgagg aagccctcaa
      181 gtttgcgtgc acgtacaggg tgctgaagtt tgcgaatagc ctttgcttca acctggcgaa
      241 cacgttcgcg cgtaaccttg aaaatgcggc ctacttcttc cagcgtatat gtgtatccgt
      301 cgccgatacc ataacgcaac ttgataatct cgcgttcacg ataagtcagc gtcttgagca
      361 cgtcttcaat gcggtctttg agcatttccg agccggctga ctccaacgga ctttcagcgg
      421 tttcgtcttc aatgaaatcg ccgaaataac tgtcttcgct ttcgccgacc ggcctgtcga
      481 ggctgatagg atgacgcgaa attttcatta cgcggcgtgc ttcagacacg ctcatgtccg
      541 cttcgtgtgc gatttcttcg atggtaggct cgcgacccag cgactggatg agttttttgg
      601 aaatattccg caatttgctc atggtttcaa tcatgtgcac cggtatgcgg atggtacgcg
      661 catggtcggc aatggcacgt gtgatagcct gacgtatcca ccacgtggca tatgtgctga
      721 acttgtatcc gcggcgatat tcgtatttgt ccaccgctcg catcaagccg gtgttgcctt
      781 cctggataat gtccaaaaag ctcagcccgc gattgcggta ttttttggcg atggaaacga
      841 ccagacgcag attgccgccg gataattcac gtttcgcctg ctcgtaatca ttaaaagcct
      901 tgcgtatgga tttgacgcgc ttgcggagca tgtccggctc ttcatgaacc aggtcgataa
      961 ggccctgaag ctcttcgcgc atgacatcca cgtcttcctg cggggtgttc ttgcgaggcg
     1021 cgatttcgat ttccttttcg atattggaca tcttgtcgct gaactgatta agttttttca
     1081 tcagcggctg aatgcggctg gttcgcaacg aaagttcttc gagcaatttc gcagcgcggc
     1141 gtctgcgacg ccagagaatt ttggtctgtt tctttacttc ggcgacatcc cggccggtga
     1201 cggaagtttt ataagcaccc caattttcat tgttggccac aaggactttt tccagcgtcg
     1261 tcaggttcgt aggcatacgg tcagtgattg tttccttgga aagattttcc gcactggata
     1321 ttttcatcgt acggtcgaaa ggcaaatcgc ccgaatgcac ctgtgaaaga atgttcagcg
     1381 cttcgcgtat gcagtagtca ctttccagaa cacgacggcg gaaatgcatg cgcgtcatct
     1441 caattttctt ggcaagcgat atttcctgat cgcggcggag cagcgggatt tcgcccatct
     1501 gggtaaggta catccgcaca gggtcgtcta tgcggcggga ttccatggcg agttcttcac
     1561 tgagctggtc ttcaggcgat ttctcttcgt catcttcgag cgagtcgtcg ctgaacttgt
     1621 cggactcatc ggcaatatcg gcttcatcca cgagctcaat gccaagctca tccagtgtca
     1681 ttagcaatga gtcgagtctt tctggcgtaa cggcactgtc cggcaaatca tcgttcatct
     1741 cttcgtaggt cagataacca cgttccttgc ccttagcgat gagttttttt acgctgatat
     1801 ccagtatttc gctcataacg cctttatccg tgtctgaaat aatttcaggc tgtttatttt
     1861 ctccactggg agttttttga ggcacgatag tgttccttaa aaagtggttg tgccggatca
     1921 gcctgggcca aatcgaattc cgtttactcg ccaacgtccg gctcctagcc agacgttccg
     1981 ttacaacacg tgaaaacatc ggattctagc aatctgcacg gctgtggtca aggcatattc
     2041 aacacattag ccatattata cgagttatac gagtgccgcc accaatcctg ccacggaaca
     2101 ttccgcagcc acgacatccg ccggaaaacc cagctcctgc aacttcatgg cggtaacagg
     2161 gccgatggca gcctttttta tattatttat aatcctcaaa aaggcctgag gttccgcttt
     2221 tttcaacagt ttcacaaaat tttccgcagt cgacgacgat gtgaatgtga taatatcaac
     2281 atggccctgc cgcagggcat ttatcgcatc gggcggcaat tcggcagggc agcgcgtaca
     2341 gtaagcgcac acttcgtcaa cttttgccgc agccggctca agagcgtttt tcaaatccgg
     2401 cggagcgata tcgctgcgag ccagaagtac agtatttcca ggagttaatc gcaatttcag
     2461 gtcagccgcc aagccagccg tcgtataatt gcatttcggc gtcaaatctg ccagcaaacc
     2521 gaagtcagcc aatttcccgg ctgtggcagg ccccacagcc gctattttca caccagccaa
     2581 cgccctggca tccaagccag ccaccgccag acggctcata aaaaaaatca caccattcgg
     2641 actggtaaaa accagccaat cgtaactggc caaatgttca atcgccgcgt cgagcgggct
     2701 gaaattttca ggcggagcga tttcgatagt tggggtttcg ataacttccg caccgctgcg
     2761 gcggagcatg ccggcgagcg ggactgactg ctttagcggc cgcgtaacta atacggtttt
     2821 gccggctaac ggtagttttt cataccagcc gcgtgctttc gccgcagcct gcccgacgat
     2881 aagtaccgcc ggcggttcaa cgccggcatc gcgggttttc ccaaaaatat caccaagtgt
     2941 gccaatcagg gttttctgcg acggccgccc ggccatccgc acgacagccg ccggcgtatc
     3001 tgccgccctg ccagcggcaa tcagtcctgt cgttatcggc tcaagatgac cgactcccat
     3061 gtaaaatact accgtatcga tacccgcaag cgcagtgtag ttgagagagc tggtctgctt
     3121 ggccgggtct tcatggccgg tgacgaacgc cacgccggac gcatagcgcc ggtcagtcaa
     3181 gcttataccg ccgtacgcac cggcagctat cgccgccgtt ataccgggca caacacgata
     3241 ctgacagccg gctgcctcca gcgcgtcaag ttcctcgccg ccgcggccaa aaatgaaaca
     3301 atcgccgcct ttcagccgta cgacaagtct gccctccagc gtatatttaa ccaga
//
LOCUS       DAONFI010000264         3355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35163_length_3355_cov_3.782727, whole genome shotgun sequence.
ACCESSION   DAONFI010000264 DAONFI010000000
VERSION     DAONFI010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35163_length_3355_cov_3.782727"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..672)
                     /locus_tag="PLK08_08285"
     CDS             complement(211..672)
                     /locus_tag="PLK08_08285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPS53536.1"
                     /translation="MTWLGQLGFALPLGMGAVGSALGIGVAGSAAAGAWAKLAKQGKS
                     LDAKYLAMVGMPLSQTLYGFLVMFVILKTRVYDPQTVAANAGALFSIGLAAGLGELLS
                     AWLQGVIGAAGIRAMSESEGAPFAFTLIAMGIAETVGIFSFVLMFLMLPAA"
     gene            complement(681..2597)
                     /locus_tag="PLK08_08290"
     CDS             complement(681..2597)
                     /locus_tag="PLK08_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53537.1"
                     /translation="MIAKMSKVFVAASADKSDDFLKALRKLGVVHIVPVDADKAVPDN
                     ETLSKLNSLKTAIDTLEFVVPAGQKPQLDSMQAAEKVCDIIRREQELHNRLAAVRRNL
                     LQIEAWGDVSVESIRRLEESGLKLMLASITAAELEELKKTAQCVTVLQENKKNTLTVA
                     AVYKNSDDVTLPPSAVEIERPKIDAPTLRRQADEIKSQMEQDTEALSAAALMLDEIRR
                     EYARVDAKARFIVARNSALAGEKLFAVQGWIPSDIADSLASKLDTLGVVAAVGVMPPE
                     EGENPPTLIRYHRWVKPIIALFDILGTVPGYKEYDMAPFFMLAMPVFTAMLVGDAGYG
                     LIFTLVGLIFGRKLHRLTGNKSAGQLITIFGIFTIIWGVITANYFGVGPSDIAKAGGY
                     GCIVKGEFQPDYHAMEIADGGWADFGKIMLAPAVLWRPNDSDARDIVMKISFIIAVLH
                     LMLAHMRKMVGLFPSQQFIAEAGWMIFIFGMFELVWLMFFGAPPVGSTTLMIYFLAVG
                     MLLFSLFSFPSPNPVKRVGFGIMTNLLPIISSFGDTLSYIRLMAVGLASYYMASAFNG
                     LAMKIFHADWYLAWTGVLILLAAHALNIFLCVIAIFAHGVRLNLLEFSNNCGVEWAGY
                     PYTPFYDTLEKQGE"
     gene            complement(2594..3256)
                     /locus_tag="PLK08_08295"
     CDS             complement(2594..3256)
                     /locus_tag="PLK08_08295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPS53538.1"
                     /translation="MAAKIKLTRPELKRQRDALRRFQRYLPMLKLKQQQLQMMVRRMA
                     GEVQAAFDAINAARRKFAPIKPVLVENAGVKFDTLAKPSQVNTSLQNVAGVNIPVFSD
                     VEFPAVSYSLFATPAWVDETLRVMREISRRQAEYDVRSRALQLIERELTKIVQRVNLF
                     EKVKIPEAKEAIRRIRIKLGDEMTAAVGRSKIAKAKISVSEETIYDMPVGHVHNEGGD
                     DE"
BASE COUNT          871 a          932 c          771 g          781 t
ORIGIN      
        1 accactataa tcttgtaata cacaagtgac aattctacgt aaaaaaaata aaaaactgcg
       61 ttaatatatc cttcgtaatt ctacagttct tgcactgtga ataattcaat caccgccaaa
      121 tgcataagca aaagtcttct tttatataca accatatgat gctacggttt taactcgcag
      181 aagtttcaac tgtcacatct gtccgtatta tcatgccgcg ggaagcatga ggaacatcag
      241 cacgaatgag aatatgccga ccgtttccgc aatacccatg gcgataagcg taaatgcaaa
      301 cggcgctcct tcggactcgc tcattgcgcg aatgcccgcc gcaccgataa cgccctgcag
      361 ccatgcactc agcaattcgc ccaagccggc ggctaagccg atgctgaaaa gtgcgcctgc
      421 attcgcggca accgtctgcg ggtcgtaaac gcgggttttc agtatgacaa acatcaccag
      481 aaaaccatac agcgtctgcg aaagcggcat gccgaccatg gcgagatatt ttgcgtcaag
      541 gcttttgccc tgtttggcga gttttgccca tgcgccggcg gccgcactgc cggctacgcc
      601 gataccaagc gcactgccaa cagcacccat gccaagcggc agtgcgaacc caagttgacc
      661 taaccaagtc atatccattt ttactctcct tgtttttcca gagtgtcgta aaaaggcgtg
      721 tagggatagc cggcccactc cacgccgcaa ttattcgaaa attccaacag gttcagtcgt
      781 acaccgtgtg caaatatggc gatgacgcac agaaaaatgt tcaacgcatg tgccgccaac
      841 aatataagta cgcccgtcca cgccagatac cagtccgcgt gaaaaatttt catcgcaagg
      901 ccgttgaatg cggacgccat ataataactc gccaacccta ccgccatcag acgaatataa
      961 ctcagcgtat cgccgaatga acttattatc ggcagcaggt tggtcattat gccgaacccg
     1021 acccgtttga cgggattggg cgacggaaaa ctgaacagag aaaacagcaa catgccgaca
     1081 gccaggaagt atatcatcag ggttgtactg ccaaccggcg gtgcgccgaa gaacatcagc
     1141 cacaccaact cgaacatgcc gaaaatgaat atcatccagc cggcttcggc gataaactgc
     1201 tgagacggaa acaggcctac catctttcgc atatgtgcca gcatcaggtg caatactgcg
     1261 ataatgaaac ttatcttcat cactatatcg cgagcgtcgg aatcgtttgg ccgccaaagc
     1321 acagcgggcg ccagcatgat tttaccgaaa tcggcccagc cgccgtcggc tatttccatc
     1381 gcgtgataat ccggctgaaa ctcacccttg acgatacagc cataaccgcc ggctttggca
     1441 atgtcggacg gcccgacgcc aaaataattg gcagttatga caccccatat tatggtaaat
     1501 atgccgaaaa tcgttataag ctggccggca cttttattgc cggtaagcct gtgcaatttc
     1561 ctgccgaaaa tcagaccgac cagcgtaaat atcaatccgt aacccgcatc gccgacgagc
     1621 atggcggtaa acaccggcat cgccagcatg aaaaacgggg ccatgtcata ttctttataa
     1681 cccggcacag tgccaagaat atcgaacaat gcgataatgg gtttgaccca gcggtggtaa
     1741 cgtatcaatg tcggaggatt ttcgccctct tccggcggca ttacgccgac agcggcgaca
     1801 acgccaaggg tgtccaattt cgacgcaagc gagtcggcta tatcggaagg aatccaaccc
     1861 tgaaccgcga aaagtttttc gccggcaagc gcgctgttac gggcaactat aaatcgggcc
     1921 ttagcgtcga cacgagcata ttcacgacga atttcatcca gcataagtgc ggcggcagat
     1981 aatgcttccg tatcctgctc catctgcgat tttatctcgt cagcctgtcg gcggagagtg
     2041 ggggcatcta ttttaggtct ttcgatttcc acagccgacg gcggcagcgt cacatcatct
     2101 gaatttttgt atacggcggc tacagtcagg gtatttttct tattttcctg caacacggtc
     2161 acgcactgcg cagttttttt caattcttcc aattcggcgg cggttatcga agcgagcatc
     2221 agtttcaggc cgctctcttc aagtcggcga atgctttcga ctgaaacatc accccatgcc
     2281 tcaatctgta aaagattccg gcgaaccgcg gcaagccggt tatgcaattc ctgttcacgg
     2341 cgaataatat cacaaacctt ctcagcagcc tgcatcgagt caagctgagg tttctgcccg
     2401 gccggcacaa caaattccag cgtatctatc gccgttttga gactgtttag cttcgaaaga
     2461 gtttcattgt cgggaacggc tttatcggca tcaaccggca ctatatgcac aacacccaat
     2521 ttgcggagcg ctttgagaaa atcatcgctc ttatcggcgg aagccgcgac gaacaccttg
     2581 gacatctttg ctatcattcg tcgtcacctc cttcgttatg cacatggccg acaggcatat
     2641 cgtaaatggt ctcctcgctg actgagattt ttgccttcgc tatttttgaa cggccgacgg
     2701 cggcggtcat ctcgtcgccc agcttgattc tgatacgacg aatagcctct ttggcttcag
     2761 gaattttgac cttctcaaac aggttgacgc gctgaacaat tttagtcagt tcacgttcga
     2821 ttagctgtaa cgcgcggctg cgaacatcgt actcagcctg acgtcgcgat atttcacgca
     2881 taaccctgag cgtttcgtcc acccacgccg gcgtcgcaaa gaggctatac gaaacagccg
     2941 gaaactcaac gtcgctgaaa accggtatat tcacgccggc gacgttctgg agggatgtat
     3001 tcacctgcga cggcttggcg agagtgtcga atttcacacc ggcgttttcc accaaaacag
     3061 gtttaatcgg ggcgaatttc cgtcgcgcgg cattgatggc gtcgaacgca gcctgcactt
     3121 cgccggccat tcgacgcacc atcatctgca actgctgctg cttgagtttg agcatcggca
     3181 gataacgctg aaaacgtcgc agcgcatcac gctgacgttt gagttccggc cttgtcaatt
     3241 ttatcttagc agccatcgtt atatctcaaa aatccgaaca tcaaaaaagt aaaaacctct
     3301 actgacccag acagctcaca aaaagtcttc cttctgtgaa ccgctctaaa tcatg
//
LOCUS       DAONFI010000265         3334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35484_length_3334_cov_4.558707, whole genome shotgun sequence.
ACCESSION   DAONFI010000265 DAONFI010000000
VERSION     DAONFI010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35484_length_3334_cov_4.558707"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /gene="yajC"
                     /locus_tag="PLK08_08300"
     CDS             <1..232
                     /gene="yajC"
                     /locus_tag="PLK08_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPS53539.1"
                     /translation="RKEMIEALKKGDKVVTIGGICGTIVEAKPTEIVIKVDDNTKIKF
                     ARWAVRAAGEDAAADKKKDTNDQQNASCNNNA"
     gene            249..1520
                     /gene="purD"
                     /locus_tag="PLK08_08305"
     CDS             249..1520
                     /gene="purD"
                     /locus_tag="PLK08_08305"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPS53540.1"
                     /translation="MDVLLVGSGGREHALAWKIMQSPLCDKLYAAPGNPGIAADGGEC
                     VNISADDVQGVVNFARDKKVGIVVVGPEAPLAMGMGDAVRNAGMLCFGPVANGARIES
                     DKAYAKQLMQNVSVPTAEARIFTDFESAKTYVLSRDHGIAVKAAGLAAGKGVIICPEP
                     FQAVKPLEEIMNDKVFGDAGNVVLIEELLTGQEATVLAFVDGRNIYPLEPLQDHKPIG
                     DGDKGLNTGGMGAYSPVPIVDSKAMDRIEREILVPIVDAMRRDFGRYEGVLYTGLMFT
                     PAGPKVIEFNCRFGDPECQPLMMRFKSDLLEAMIATAEHRLDEVTIEFDTRPSLCVVM
                     SSAGYPGKYEKGKVITGIEEANAIDGVKVFHAGTAMKDGRLVTSGGRVLGVTAIADTL
                     AEAQEKAYGAVTKIQFEGAYWRNDIADKAFK"
     gene            complement(1612..2655)
                     /locus_tag="PLK08_08310"
     CDS             complement(1612..2655)
                     /locus_tag="PLK08_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS53541.1"
                     /translation="MTEQSNRTIPDFSLLPARRRDEPMPVKYWKKVGIMLTDWCNASC
                     SSCYLYCNPSRNNWMSAADAIRVWQELREISPHGCKVHLTGGEPFGNYELLLKITQEA
                     TAKGLGQTDKIETNGFWAANADIVRKRITELDAAGMRLLAVSCDPYHQQFVPMKTVRL
                     LVRTAEEIITPQRVQVRWRDYLEMGCDTNLLEEPQRDRLYMDWIKKDRDRISGRAADV
                     LSSYMEKYPPETFAGQDCRDSLLKSGNIHVSPTGVVLPGVCSGLCLGNALQEHVGDIW
                     SRVYKSHNDMPILGTLANSGPFGLLDIAKKTGFRSDPLGYAGKCHLCWEIKKYFAQNH
                     MFENELCPVLAIL"
     gene            complement(2660..>3334)
                     /locus_tag="PLK08_08315"
     CDS             complement(2660..>3334)
                     /locus_tag="PLK08_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53542.1"
                     /translation="KEKQRKEAMEKQSEELEKVIDKNYEKNAEDEELNDLSLQHDKEL
                     RKQMIVTLDKTRNVVLKNLAGNSKDLFNRYMNNAKRALAKGRYYAASQQYELAAITNS
                     RNPLPWLGGSLAKFAACEWSVAGFRLQTAMETYPQLMEVKLDLKSLLPNMNVQQQLNL
                     LENWLIAITPQPNVLVLAAYMQYQAGNIEGARRHAQAILNYPSKVAPIIKYVAEEIMK
                     KTAPKK"
BASE COUNT          777 a          817 c          906 g          834 t
ORIGIN      
        1 acgcaaggaa atgattgaag ctctcaaaaa aggcgacaaa gtcgtgacca tcggcggaat
       61 ctgcggcacg attgttgagg ctaagcccac ggaaatagta atcaaggttg acgacaacac
      121 taaaataaaa ttcgcacgat gggctgttcg ggctgccggt gaagacgcgg ccgctgacaa
      181 gaaaaaagac accaacgacc agcagaacgc atcctgcaac aacaacgcct gattgggact
      241 acagactcat ggacgtatta ctggtaggta gcggcggcag ggaacatgcc ctcgcctgga
      301 aaatcatgca atcaccgctt tgtgacaagc tctatgctgc gccgggtaac cctggcatag
      361 cggccgacgg cggcgaatgt gtgaatataa gtgccgatga tgttcagggc gttgtaaatt
      421 tcgccaggga caaaaaggtc ggcatcgtag tagtcggccc cgaagctccg cttgcgatgg
      481 gcatgggcga cgcggtacgc aatgctggaa tgctctgttt tgggcctgta gccaatggag
      541 cacgcattga aagcgataaa gcctacgcca aacaacttat gcaaaacgta tccgttccga
      601 cggcggaagc cagaattttt accgacttcg aatcagcaaa gacttatgtg ctcagccgag
      661 atcacggcat agcggtaaaa gcggctggct tagccgccgg caaaggcgtc ataatctgcc
      721 ccgaaccttt tcaagccgtc aagcccctcg aagaaataat gaacgacaaa gtcttcggcg
      781 acgccggcaa tgtggttctg atagaagaat tgctcaccgg ccaggaggcg acggtgcttg
      841 cttttgtcga cggacgaaat atttatccac ttgagccgtt gcaggaccac aagcccatcg
      901 gcgacggcga taaaggcctg aacaccggcg gcatgggtgc gtattcgcca gtgccgatag
      961 tggactcgaa ggctatggac aggattgagc gggaaatcct tgtgcctata gttgacgcca
     1021 tgcgtcgcga ttttggccgt tatgaaggcg tgctttatac cggcctgatg tttacgccgg
     1081 ctggccctaa ggttatagaa ttcaactgcc gcttcggaga ccccgaatgt cagccgctca
     1141 tgatgcgttt caagagcgat ttgctggagg caatgattgc cactgctgaa catcgtctgg
     1201 atgaagttac catcgagttc gacacgcggc cgagcctttg cgtggtgatg agttccgcag
     1261 ggtatcctgg caagtacgaa aaaggtaagg ttataaccgg catcgaggaa gcaaacgcca
     1321 tcgatggcgt taaggtcttt cacgccggca cggctatgaa agacggcagg ctggtgacta
     1381 gcggcggacg tgtgctgggc gtgacagcca tcgccgacac gctagccgag gcgcaggaaa
     1441 aagcatacgg cgcagtgacc aaaatacagt ttgaaggcgc atactggcga aacgatatcg
     1501 ccgacaaggc attcaaatga taaataacaa gtaacaagag ccaagtaaca aacaaatttg
     1561 aaatctcaat tttcaagaag cgtggccgac acaggttacg cttctttttt tttacaaaat
     1621 cgccaaaacc gggcatagtt cgttttcaaa catgtggttt tgggcgaaat attttttgat
     1681 ttcccagcag aggtgacatt tgccggcata gcccagcggg tcggaccgga agccagtctt
     1741 ctttgcgata tccagaagcc cgaacggccc actattcgcc agcgtgccga gaatcggcat
     1801 atcattgtgg gatttatata cgcgggacca tatatcgccc acgtgttcct ggagtgcgtt
     1861 gccgaggcag aggcctgaac atacgcctgg cagaacgacg cctgtgggtg aaacgtggat
     1921 atttccgctt ttgagtagtg agtcgcggca atcctggccg gcgaaagttt ccggcggata
     1981 tttttccata tatgaagaaa gcacgtcggc tgctctgccg ctgatgcggt cacggtcttt
     2041 ctttatccag tccatgtaca gcctgtcacg ctgtggttct tccagcagat tggtatcgca
     2101 acccatttcc agatagtcgc gccatcttac ttgaaccctt tgcggggtga tgatttcctc
     2161 cgccgtgcgt acgagcagcc gtacggtttt catcggtacg aactgttgat gatacgggtc
     2221 gcacgatact gccagcagac gcatgccggc ggcgtcgagc tcggttatgc gttttctcac
     2281 gatatcggcg ttcgctgccc agaagccgtt agtttctatc ttgtcagtct ggcctaaacc
     2341 tttagccgtg gcctcttgcg tgattttcag aagcagttcg taatttccga acggctcacc
     2401 gccggtcaga tgcaccttgc agccgtgggg ggatatttct ctgagttcct gccatactct
     2461 tatggcgtca gctgctgaca tccagttgtt tctggacgga ttgcaataca ggtagcatga
     2521 tgaacagctg gcgttacacc agtccgtcag cattatgccg acctttttcc aatatttcac
     2581 gggcatgggt tcatcgcggc gtcttgccgg cagtaatgaa aaatctggaa tggtgcggtt
     2641 ggattgttct gtcattattt tattttttcg gagcggtctt tttcattatc tcttcagcta
     2701 cgtacttgat gataggtgcg acttttgaag gatagttcaa tatggcttga gcgtgtcggc
     2761 gggcaccttc gatattgccg gcctgatact gcatgtaggc ggcaagaacc aatacgttcg
     2821 gctgaggcgt aatggctatc agccagtttt caagcagatt caactgctgc tgcacgttca
     2881 tattcggcag aagcgatttg aggtctagtt tcacttccat aagctgagga taggtctcca
     2941 tggcggtttg cagtctgaag ccggctacgc tccattcaca tgcggcaaac tttgccagag
     3001 agccgcccag ccacggcagc ggatttctgg aattcgtgat tgcggcaagt tcatattgtt
     3061 gcgacgcggc gtaatagcgg cctttggcga gtgcacgttt ggcattgttc atataacggt
     3121 taaaaaggtc tttgctgttg ccggctagat ttttaagcac cacgttacgg gttttatcca
     3181 gggtgactat catctgcttt cgcagttcct tgtcgtgctg gaggctgagg tcattgagtt
     3241 cttcatcctc ggcgttcttt tcgtaatttt tgtctattac cttttcaagt tcctcggact
     3301 gtttttccat agcttccttg cgctgttttt cctt
//
LOCUS       DAONFI010000266         3334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35487_length_3334_cov_3.674291, whole genome shotgun sequence.
ACCESSION   DAONFI010000266 DAONFI010000000
VERSION     DAONFI010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35487_length_3334_cov_3.674291"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..677
                     /locus_tag="PLK08_08320"
     CDS             204..677
                     /locus_tag="PLK08_08320"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HPS53543.1"
                     /translation="MRWIWIDKFVEFEPGRRAVSIKNVSLAEEHLHDHWQAFPVMPAS
                     LMIEGMAQTGGILVGQANNFEHDVLLAKVTKAEFEGIALPGDQLTYEAEIENMAPEAA
                     SIKGTVLKNGAPFGSISIMFSHADNNIQGLDLPQGFVFTGSFQRMVEPFLETGNG"
     gene            670..1989
                     /locus_tag="PLK08_08325"
     CDS             670..1989
                     /locus_tag="PLK08_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPS53544.1"
                     /translation="MAEKRVVITGLGIVSPAGAGIEENFQKLLAGHSAVKKIERFDPS
                     GFECRIAGEMAEFSARNYVPKGYRKAVKVMARDIEIAVAAADLAFRDADIITRERAKE
                     ENVQMQIEPSRLGCNIGAGLICAELDELGMAATTSKDADGNFDFKLWGEHGMTNLTPL
                     WLLKYLPNMLSCHVTIIHGAEGPSNNITCGDASGPLSVGESVTYIKRGSADAVVTGAA
                     ESKLNPMGVMRQSKLGRVCKIGNDHPATAIRPFDAAHAGLAPAEGGALVILEDFSRAT
                     SRGVRIYAEVAGFGAACDPKGMDITRPTAGNLGLAVKAALKQAGITPDDVDMIVAYGT
                     AVPGEDAAEAAEWKKAFGEKLAQIPAVSFTGLTGTMYAGGGAAQLALAAKAVEQQIVP
                     PTINFEKPADGCELNLSAKARKKDIRYAVVGAFTVGGQSGAVVLKKA"
     gene            2152..>3334
                     /locus_tag="PLK08_08330"
     CDS             2152..>3334
                     /locus_tag="PLK08_08330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPS53545.1"
                     /translation="MGQNRVVITGIGTVNPLAHDAEGFWQAILACKLAPSANELFDAS
                     TYPTKFAAQVRNFNLADYLTGESLQRHKTAGRHCQFALAAAVEAWKMSGLENCQNLNH
                     ERLGVYLGSGEGSLDFDNFIAAILGSWNGNGIDTVKWAQLAMKSMNLHRELEQESNMV
                     VSHLAGEFNAMGPAFNVLTACAASTQALGEATLQVRNGLADVMISGGAHSMIHPLGVT
                     GFNRLTALSQRNDDMPHSSRPFDLTRDGFVLGEGASILILENYEHAKARGAKMLCEVI
                     GYGASADAFRITDQDPAGDGAAAAMRAALTDAELSPKDIDYVSAHGTGTKQNDQVETL
                     AMKQVFGQTKQEVPPVSSVKSMVGHLIAAAGATELITCILAIRDQILPPTANLHTPDP
                     DC"
BASE COUNT          848 a          910 c          941 g          635 t
ORIGIN      
        1 gcgaattcga gaaaaaccct gacgtcagca agatggctga acttttcaag gttcagacca
       61 taatcaacta tgtcatgggc aaaattaacg gctgaacctg agagtttgac ggaaaatatt
      121 gcaagggatg gcgacaccgt gaacagttcc gccgacgaac ggcggacggg tgtggtgttt
      181 ccatcccttg tctggactaa tgaatgcgtt ggatatggat tgacaaattt gtggagttcg
      241 aacccggccg tcgagcggta tcgataaaaa acgtctcgct ggcggaggaa catctgcacg
      301 accattggca ggcgtttccc gtcatgccgg cgtctctgat gatagagggc atggcacaga
      361 ccggcgggat actcgtcggc caggcgaata attttgaaca cgacgtactg ctggctaagg
      421 tgacgaaagc tgaattcgag ggaatcgccc tgccgggcga ccagcttacc tatgaggcgg
      481 aaatcgaaaa catggctccc gaagcagcca gcataaaggg cacagtgctt aaaaacggcg
      541 ccccgttcgg cagcatatcc ataatgttct cacacgcaga caacaacatt caaggcctcg
      601 atttgccgca aggttttgtt ttcaccggtt ctttccagcg catggtagaa cctttcctgg
      661 agactgggaa tggctgaaaa acgcgtcgtt atcacaggac tcggaatcgt cagccctgcg
      721 ggcgcaggca tcgaggagaa ttttcaaaaa ctgctggcgg gccactcggc ggtcaaaaaa
      781 atcgaacgtt tcgaccccag cggtttcgaa tgccggatag ccggcgaaat ggccgaattc
      841 tcggcgagaa actatgtgcc aaaaggctat cgtaaagccg tcaaagtcat ggctcgcgat
      901 atagaaatag cagtcgctgc ggcagacctt gcgttccgcg acgcagacat catcacccgt
      961 gaacgggcta aggaagaaaa cgttcagatg cagatagagc cttcgcggct gggctgcaat
     1021 ataggggcag gcctgatttg cgcggaactc gacgaactcg gcatggcggc tacgacgtcc
     1081 aaagacgccg acggcaattt cgatttcaag ctgtggggcg agcacggcat gacgaacctg
     1141 acgccgctgt ggctgttgaa atatctgccg aacatgctct cctgccacgt gaccataatc
     1201 cacggcgctg aagggccgag caataatata acctgcggcg acgcatccgg cccattgtcg
     1261 gtcggcgaaa gcgtcacgta catcaaacgc ggttcagccg acgcagtcgt caccggcgca
     1321 gcggaaagca agctcaatcc gatgggcgta atgcgacaat ccaaacttgg ccgggtctgc
     1381 aaaatcggca atgaccaccc agccacggca atacggcctt tcgacgcggc acacgccggc
     1441 cttgctcctg ccgaaggcgg agcgctcgta attctggaag acttcagccg tgcaacatcg
     1501 cgcggcgtac gaatatatgc tgaagtagcc ggcttcggag cggcatgcga cccgaaaggc
     1561 atggacataa cgcggccgac agccggtaat ctggggttag ccgtcaaggc tgctctcaaa
     1621 caggctggca ttacgccgga cgacgtggac atgatagtcg cctacggcac ggctgtgcct
     1681 ggcgaagacg cggctgaagc ggctgaatgg aaaaaggctt tcggtgaaaa actggcacag
     1741 ataccggcgg tttcgttcac cggcttgacc ggcacgatgt acgccggcgg cggagcggca
     1801 cagctggctc tcgcggcaaa ggctgtcgag cagcagattg taccgccgac gataaatttt
     1861 gaaaaaccgg cagacggctg cgaactgaat ctctcggcta aggcacggaa aaaagatatc
     1921 cgttacgccg tagtcggagc ttttaccgtc ggcggccaaa gcggtgcggt cgtattgaaa
     1981 aaggcttaag cgacgcgagc agataaattc atacgggcca aaaatattag aatcataatt
     2041 ttagagatgt cgcctgtctg cctgatggcg gacactttta gccgcggttt tttctttttt
     2101 ttaaaaaccg tagaattacg aatgacatat taaaaacttt aggtaaacga catgggtcaa
     2161 aacagagttg tcataacggg tataggaacg gtaaatcccc tggcacacga cgccgaggga
     2221 ttctggcagg cgattctcgc atgtaaactc gccccaagcg cgaatgagct tttcgacgcc
     2281 agcacctacc cgactaaatt tgcagcccag gtcaggaatt tcaatctggc ggactacctc
     2341 accggcgaaa gcctccaacg tcacaaaacc gccggccgcc actgccagtt cgcactcgcc
     2401 gccgcagtgg aagcatggaa aatgtcaggc cttgaaaatt gccaaaacct caaccatgaa
     2461 cgactgggcg tatatctggg ctctggcgaa ggcagcctgg atttcgacaa cttcatagcc
     2521 gccattctcg gctcatggaa cggcaatgga atagatacgg taaaatgggc tcagctcgct
     2581 atgaaaagca tgaacctgca tcgcgaactt gagcaggaat cgaacatggt ggtttcccat
     2641 ctggccggcg aattcaatgc gatggggccg gcgttcaacg tgcttaccgc gtgtgcagcc
     2701 agcacgcagg cactcggcga agccacactt caggttcgca acggcttggc tgatgtgatg
     2761 atttccggcg gagcgcacag tatgattcat ccgctgggcg tgaccggttt taaccggctg
     2821 acagcccttt cgcagcgcaa tgacgatatg ccgcactcat cacggccctt tgacctgacg
     2881 cgcgacggtt tcgtgctcgg cgaaggcgcc agcatactga tactggaaaa ttacgaacac
     2941 gcaaaagccc gcggcgcaaa aatgctctgc gaagtcatag gatacggtgc aagcgccgac
     3001 gccttccgca taacagacca ggatccagcc ggcgacggcg cggcggctgc gatgcgagcc
     3061 gctctgactg acgcggaact atcgcccaaa gatatcgact acgtctccgc acacggcaca
     3121 ggaacgaagc agaacgacca ggtcgaaacg ttggccatga agcaggtctt cggccagacg
     3181 aagcaggaag ttccgccggt atcttccgta aaaagcatgg tcggccatct catagctgcc
     3241 gccggcgcta cggaactcat cacgtgcata ctcgccatcc gcgaccagat actgccgccg
     3301 acggcaaatc tgcatacgcc ggaccccgac tgcg
//
LOCUS       DAONFI010000267         3330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35545_length_3330_cov_3.416183, whole genome shotgun sequence.
ACCESSION   DAONFI010000267 DAONFI010000000
VERSION     DAONFI010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35545_length_3330_cov_3.416183"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..2040)
                     /locus_tag="PLK08_08335"
     CDS             complement(52..2040)
                     /locus_tag="PLK08_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS53546.1"
                     /translation="MAKKGYFRQFVEMADMKRVPDEYPRSKNSHADVLYIMKRFYSPF
                     MMPLILLSVIAMILSYSQFFYTWCTRYATDNIVQINLADKPTRTIIDPSAMDESRRFE
                     ISDSNRNTSWSGRWDNFGGLLRSQKISRLIFLLVVACTWVGIDYLGNRVYGTIMARIS
                     RKVQYRMRHRIYDSLHALPMEYHDRQAVGSLMTNLFTDVQVIVDSVNILHHHSVFYLC
                     SIIIGAFILLSIDTRLAMITFCSMPFYLITYKWFRSRLMVVYDNLRYQEGMLNAYVNN
                     RIKNFYLVKSFVRELGEVRNFMGKTREIIHNSLGANVLGTLFTVACGIISGFCLVIIL
                     YMGTNAVFSGKITTGELLMFYTSAGLLFNPIAVLSSLVSQYHILATRCQKVHRLLNQP
                     ITLANVQNPIQIPAAMAELRFEDVVMHYGSARNPALDGISFTLPAGAKMCVMGSSGSG
                     KTTLAKLSARIYDPVQGRILLDGHDIKEYRLQELRQYIGYVNQEPIIFDGTLKANIAY
                     GSYGSQPQEIMTAARHAQIHDYITTLPERYETITAERGLTLSGGQKQRVNLARVLLAD
                     PQLLILDDCTSALDAETEARLIKEFDQVLKNRTAMIVSHRISMALQCDYVMLLKNGKI
                     LEFGEPEELLEQSGEFKRMMDEQIARSAMNLFKKEGAA"
     gene            complement(2056..>3330)
                     /locus_tag="PLK08_08340"
     CDS             complement(2056..>3330)
                     /locus_tag="PLK08_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPS53547.1"
                     /translation="KMTLIVLTAVGLYVVNYKYFVPRIRRLQRRYQCKMDNLSAIAQE
                     KLSGIVVVKSFGNERFESREFTKKNFAAERVLHRIMILNNRYGSFIVLLASGTYPILL
                     IFGTYLAIDGQMTFGAVTAACAFAVRLLTPAAMLAEFSNQIQQGKVSLARIFELLQAD
                     EDLFNPAGYKPHAKLIGEVEFRDVSFHYRPQKPVLRNINLKIQPGWTVALVGQTGCGK
                     STIINLLYRFYDVCGGSLLVDGKDIREYDTQAYRRNLAIVPQEPIILDETIANNIAYG
                     RPDASREEIRRAAQMAELEEVITRYPQGLDTLLGEKGIRLSVGEKQRICIARAILADP
                     AILILDEATSSLDTKSETMIQSAMKKVMAGRTNFVVAHRLSTIVDADLIVVLEDGQIT
                     EMGNHRQLLNRPDGKYHKLYMTQIAASPYVAV"
BASE COUNT          773 a          835 c          838 g          884 t
ORIGIN      
        1 atagctcgtt tcgccaacgc ataaagtgtc gtatctcaag tttaacatgc tctaggctgc
       61 gccttctttt ttaaacaggt tcatcgccga gcgtgcaatc tgttcgtcca tcattctctt
      121 gaactcgccc gactgttcaa gaagttcttc cggctcgccg aattccaata ttttgccgtt
      181 cttgagaagc atgacgtaat cgcattgcaa cgccattgat atgcgatgcg aaacaatcat
      241 cgcggtacga tttttgagca cctggtcgaa ttcttttata agcctggctt ccgtttcggc
      301 atccagcgcg ctcgtgcagt cgtcaagtat cagcaactgc gggtcagcca gcaggacacg
      361 ggcgagattg acgcgctgct tctggccgcc ggaaagcgtc aaaccacgtt cggcggttat
      421 ggtttcatat cgctccggca gggtggttat gtagtcatgt atctgtgcgt ggcgggctgc
      481 ggtcattatt tcctgcggct gagagccgta tgagccgtat gcgatattgg ctttcaatgt
      541 gccgtcaaat ataatgggct cctggttgac gtaaccgata tattgccgca attcctgtaa
      601 ccggtattcc tttatatcat ggccgtcgag aagaatgcgg ccctgtaccg gatcataaat
      661 tctcgcagat aacttggcca gcgtggtttt gcccgacccg cttgaaccca tgacgcacat
      721 ttttgcacca gccggcaacg taaagctgat gccgtcgagt gccggattcc gggctgagcc
      781 gtaatgcatc actacatctt caaaccgcag ttccgccata gctgccggta tctgtatcgg
      841 gttctgcacg ttcgcgagag ttatgggctg attcaggagg cgatggactt tctggcatcg
      901 tgtggcaagg atgtgatatt gcgatacgag gctgctgagg acggctatcg ggttgaacag
      961 caggccggct gacgtataaa acatcagcag ttcaccggtg gttattttcc cgctgaatac
     1021 agcattcgta cccatgtaga gaattatcac caggcaaaag ccgcttatta tgccgcatgc
     1081 caccgtaaaa agcgtaccga gtacgtttgc cccgaggctg ttgtgaatta tttcacgggt
     1141 tttgcccatg aaatttctga cttcgccgag ttcacgaacg aaagatttta caagatagaa
     1201 attttttatg cggttgttga cataggcgtt aagcatgcct tcctgatacc gcaggttgtc
     1261 gtataccacc atcaggcggc tgcgaaacca cttgtatgta atcaggtaga acggcattga
     1321 acagaaagtt atcatcgcaa gtcttgtatc gatggacagg agaatgaatg cacctatgat
     1381 aattgaacac aaataaaaaa cgctgtggtg atgtaagatg ttcaccgaat ccacaattac
     1441 ctgaacgtcg gtgaacaggt tggtcatgag cgaaccgaca gcctgcctgt catgatattc
     1501 catcggcagg gcatgaagcg aatcgtatat tcgatgtctc atgcgatatt gtacttttcg
     1561 ggatattctg gccattatcg tgccgtacac cctgttgccg agataatcta tgccaaccca
     1621 tgtacatgcc acgaccagca gaaaaatcag ccgtgaaatt ttctgcgacc gtaaaaggcc
     1681 gccgaaatta tcccatctgc ccgaccagct ggtgtttcga ttcgagtcgc ttatttcaaa
     1741 acgccgcgat tcgtccatgg cagacgggtc gattattgtc cgggtcggct tgtcggccag
     1801 atttatctgc acgatattgt ccgtggcgta tcgcgtacac caggtgtaga agaactggct
     1861 gtacgaaagt atcatggcga ttacgctcag gaggatgagc ggcatcatga aaggactgta
     1921 aaaccgtttc ataatataca aaacgtcggc gtgcgaattt tttgaccgag gatactcgtc
     1981 cggcacacgt ttcatgtcag ccatttcgac gaattgtctg aaatatcctt ttttagccat
     2041 tctgcactta tccggttata cggctacgta gggacttgcc gctatctggg tcatatacag
     2101 tttgtgatat ttcccgtcgg ggcgattcag cagttgcctg tgattgccca tctccgttat
     2161 ttggccgtct tccagaacga caatcaggtc tgcgtccaca atggtgctaa gacggtgtgc
     2221 cacgacaaag ttcgtacggc cagccatgac ttttttcatc gccgattgta tcatggtctc
     2281 cgacttggta tccagggagc tggtcgcttc gtccagaatc agaattgccg ggtctgccag
     2341 tattgcgcgg gcgatgcaga ttcgctgttt ttcgccgacg gacaggcgta ttcctttttc
     2401 gccgagcagg gtatcaaggc cttgcgggta acgcgtgatg acttcttcga gttccgccat
     2461 ttgggcggcg cgccggattt cttcgcgcga cgcgtcaggc cggccatagg cgatgttgtt
     2521 agctatcgtc tcgtcaagaa tgatgggttc ctgcggcact atcgcaagat ttctgcggta
     2581 agcctgcgta tcatactcgc gtatatcctt gccgtcgacc agcagcgaac cgccgcagac
     2641 gtcgtaaaat ctgtaaagca ggttgattat ggtacttttg ccgcaacctg tctggccgac
     2701 gagcgctacc gtccagccgg gctgaatctt caggtttata ttacggagta caggtttttg
     2761 cggccgatag tggaaagaca catctctgaa ttccacttcg ccgattaatt tagcatgcgg
     2821 cttatagccg gccgggttga acaggtcttc atcagcctgc aaaagttcga atatcctggc
     2881 cagtgacacc ttgccctgct gtatctgatt ggaaaattca gcgagcatcg ccgccggcgt
     2941 cagcagccga acagcaaacg cacacgccgc cgtaacagca ccaaatgtca tctggccgtc
     3001 aatcgcaaga tatgtgccga aaatcaacag aataggataa gttccgctgg ctaaaagtac
     3061 tatgaacgaa ccatacctgt tgttcagtat cattatacgg tggagtaccc gctcggcggc
     3121 gaagtttttc ttggtgaatt cgcgagattc gaaccgctca ttgccgaaac ttttcaccac
     3181 cactatgccg gaaagctttt cctgagctat ggcgctgaga ttgtccatct tgcattgata
     3241 acgccgttgc agccttctga ttcgcggaac gaaatattta tagttgacta catacaggcc
     3301 gaccgccgtc agcactatca atgtcatttt
//
LOCUS       DAONFI010000268         3319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35717_length_3319_cov_3.674326, whole genome shotgun sequence.
ACCESSION   DAONFI010000268 DAONFI010000000
VERSION     DAONFI010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35717_length_3319_cov_3.674326"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1323)
                     /locus_tag="PLK08_08345"
     CDS             complement(145..1323)
                     /locus_tag="PLK08_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPS53548.1"
                     /translation="MTVKKYISDIHDELVGIRRELHANPQVGFHETFASGLVQDYLRK
                     CGIEFQAGLAETGVVGWLLPEDISKRELPGVALRADIDALTVEEKTGLTYASHNEGCM
                     HACGHDGHTVMLLGAANVLSKYRRQLTRPVKFIFQPAEESFGGALPMIRQGVLDERTG
                     GIRVNRIFAVHNWPALPLGRIGLRSGPFWASVDGFRVIIKGKGGHGARPQTAINPLIP
                     AAKAVLEIDSIKNSSLPASAGPVVLSVCSINGGTKSNVIPDDVIIQGTIRTFDDGLAS
                     EIKRRISEIAADIAAAAACKSAFEVIYGYPVVVNDSQATDEMSQAAKTVLGMENVLRD
                     YPQVSVAEDFSFYLQKVQGCIAVLGCGPSEDLHNPHYDFNDALIPTGVELFVRLAMQE
                     "
     gene            complement(1519..2190)
                     /locus_tag="PLK08_08350"
     CDS             complement(1519..2190)
                     /locus_tag="PLK08_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPS53549.1"
                     /translation="MFKEIINAFRNRDVVREMSQRIGTMLDSGKWMLEQASNVLLRKD
                     DWNAVADNLYKKDKEINAIERDIREQIITHLSLSVVGQANVIPCLVLMSVVKDAERIG
                     DYCKNIFEVGKFYRDPYRHREFIEPLEEIRTTLVNLFDPVKDAFINNREDVADACIKK
                     TAEIARKCDMIIQQLLSMQGNFDADEAVAYVLMARHYKRVSSHLGNIATSVVSAVPLI
                     DFRHN"
     gene            complement(2258..>3319)
                     /locus_tag="PLK08_08355"
     CDS             complement(2258..>3319)
                     /locus_tag="PLK08_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HPS53550.1"
                     /translation="TGLHLAATDPAGEKFLKWIFGLVSDPFTGLFVGLLVTSIVQSSS
                     FTTSMTVGLVATGDVTLVAAIPIIMGANIGTSVTALIVSLAHMGRREEFRRSFSAALL
                     HDNFNMLSVLLFFPLEYKFHIISTPVLWIAKNLAKMKMFDSDPTKQIGIFKHIFNVVG
                     DFGKWLMTDVLHMDAKIGGCVLAALAIVLLFAALGYLVKILRSVMMDRLSGAFNKTIF
                     RNQPIAFIFGLIATATIQSSSVTTSLVVPLVAAGVLQHAQIFPYMMGANIGTTATAMM
                     AAIGMGSPVAIACACAHLLFNLYGTAVFWPLQMIPINISKFIALKASRNRAIAIIFVV
                     GMYFVLPVAALTLMKMHGK"
BASE COUNT          853 a          899 c          764 g          803 t
ORIGIN      
        1 gccttttgct gaagggattc cgtaacaacc accaaaggcc tgccagccat tttcaagtct
       61 ccataataaa aacgtcccga taaagtcccg ataaatcaag ttcgcgaatt ctgaaaaccg
      121 accttgcagg ctgtaaagcc gctctcattc ctgcatagcc agcctcacaa aaagttcaac
      181 gcctgtggga attaacgcat cgttgaaatc ataatgcgga ttgtgcaaat cctccgacgg
      241 cccacaaccg aggacggcga tacatccctg aactttttgc aaataaaatg aaaaatcctc
      301 cgccacgctc acctgcggat aatcccgcaa gacgttttcc atgcccagga cggttttggc
      361 agcctgggac atttcatctg tcgcctgtga atcgttgaca accaccggat agccgtaaat
      421 aacctcgaac gccgacttac acgccgcagc cgcggcaata tcggcggcaa tttcgctgat
      481 tcgtcgcttg atttcactcg ccaggccgtc gtcaaacgta cgaatcgtgc cctgtataat
      541 cacgtcgtcc ggaatcacgt tggatttcgt tccgccgttt atactgcaaa ccgataacac
      601 aaccggccct gctgacgccg gcagcgatga gttttttatg gaatcaatct caagaacagc
      661 tttcgccgcc ggtatcaatg gattaatagc cgtctgcggc ctcgcgccat gaccgccttt
      721 gccctttatt atcacgcgaa atccgtccac gctagcccag aaaggcccgc ttcgcaaacc
      781 aatcctgccc aacggcaatg ccggccagtt atgaacggcg aaaatcctgt tcacccttat
      841 gccgccggtt cgctcatcca gcacgccctg acgtatcatg ggcagagctc cgccgaaaga
      901 ttcctccgcc ggctgaaata tgaacttaac cggcctggta agctgccgcc tgtattttga
      961 aagcacattc gccgcaccaa gcagcatgac ggtatggccg tcgtgcccgc aggcatgcat
     1021 acagccttcg ttgtgcgagg catatgtaag accggtcttt tcttcaaccg tcagggcatc
     1081 gatatccgcc cgcaaggcta ccccaggcaa ctcacgcttc gatatatcct ccggcaacag
     1141 ccagccgact acgccagtct ccgccaggcc agcctgaaat tcaatgccgc acttacgcag
     1201 ataatcctgt acaaggccgg acgcaaaagt ctcgtgaaat ccgacctgcg gattggcgtg
     1261 cagctcgcga cgaatgccga caagttcgtc atgaatgtca ctaatatatt tttttactgt
     1321 cattttgatt ttcgccgccg ttcacaaaaa aaatcatgcc cgtatttcgg gcatgatttc
     1381 aatgtaactt ataaccgtat ccgtttcaat agcacagata ccattcaaac ggcatatatt
     1441 tctagaacct atcccaaaaa catgggcgtc gccgaaatcg agcaattttg ctggctggca
     1501 aggtttttgg gacgggtttt agttatgccg gaaatctatc agcggcacgg ctgaaacaac
     1561 gctcgtggca atgttgccca gatggctgga aacacgtttg tagtgacgag ccatgagaac
     1621 ataggcgacg gcttcgtcgg catcaaaatt accctgcatg ctgagaagct gctgaataat
     1681 catgtcgcac ttccttgcaa tctcagccgt cttttttatg catgcgtcag ccacgtcttc
     1741 cctgttgttt atgaacgcat ccttgacggg gtcaaagaga ttcacaagcg tggtacggat
     1801 ttcttccagc ggctcgataa attcacgatg gcgatacggg tcgcgataga atttgccgac
     1861 ttcaaaaata tttttgcagt aatcgcctat tcgttcggca tccttcacta cgctcatcag
     1921 tacaaggcaa gggatgacat tcgcctggcc gacaacggaa agacttaaat gcgttataat
     1981 ctgctcgcgg atatcgcgtt ctatggcgtt gatttccttg tcttttttgt agagattgtc
     2041 cgcaaccgca ttccagtcat ccttgcggag caaaacgttg ctggcctgct caagcatcca
     2101 tttgccggag tcaagcatgg taccgattct ctgcgacatt tcccgtacaa cgtcacgatt
     2161 acgaaaagcg tttattattt ctttaaacat atagtcttac ctcaaattcg tattatctcc
     2221 gtcctgaacc tttcacaaat attttatata tgtatcgtca tttgccgtgc attttcatta
     2281 gagtcagcgc tgcaaccgga agcacgaaat acatgcccac cacaaagatt atcgcgatgg
     2341 ccctgtttct tgatgccttg agagcgataa attttgaaat atttatcgga atcatctgta
     2401 acggccagaa taccgccgtg ccgtaaagat tgaataaaag gtgtgcacag gcgcaggcta
     2461 tggcaaccgg cgaccccatg ccgattgcag ccatcatagc cgtcgccgta gtgccgatat
     2521 tcgctcccat catatacgga aatatctggg cgtgctgcaa gactccggct gcgacgagag
     2581 gaactaccag gctcgtagtg acggatgaac tctgtatcgt cgcagtcgca ataagtccga
     2641 aaatgaaggc tatcggctga ttgcggaaaa tagtcttgtt gaacgcaccg ctgaggcgat
     2701 ccatcatgac gcttcgcaaa attttgacca ggtatccaag tgccgcaaaa agcagtacaa
     2761 tcgccaaagc cgccagaacg cagccgccta ttttggcatc catatgcaat acgtctgtca
     2821 tcagccactt gccaaaatcg ccgactacgt taaaaatatg tttgaagatt cctatctgtt
     2881 tcgtagggtc ggagtcgaac attttcatct ttgcaaggtt tttggcgatc cacagaaccg
     2941 gcgtggatat gatatggaat ttgtactcga gcgggaaaaa cagcagcacg gaaagcatat
     3001 tgaaattatc atgcagcagt gccgccgaaa atgagcgacg gaattcctcc cgccgaccca
     3061 tatgtgccag cgaaactata agcgccgtaa cgctcgtccc gatattcgca cccattatta
     3121 taggtatggc cgcaacaagc gttacgtcgc cggtagccac aaggccgacc gtcatggacg
     3181 ttgtaaaact gctggactga acgatactgg tgaccagcag gccgacgaac aggccggtaa
     3241 aagggtcgct gacaaggccg aatatccatt tgaggaattt ttcgccggct gggtcggtgg
     3301 cggcaagatg caggccggt
//
LOCUS       DAONFI010000269         3316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_35769_length_3316_cov_3.246243, whole genome shotgun sequence.
ACCESSION   DAONFI010000269 DAONFI010000000
VERSION     DAONFI010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35769_length_3316_cov_3.246243"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PLK08_08360"
     CDS             complement(<1..1478)
                     /locus_tag="PLK08_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPS53551.1"
                     /translation="MANMNALNIPIDQLRGRPLGRVLVKMGKITKEQVYQALERQKQQ
                     SGPIGQHLVELGFIDDATLSLALGAQVGMEFVDLTTMDIPESVINLIQAQVATSYKII
                     PLEHDPDTNHLTIAVCTPNNFRAVDDLQTLMGFSGVTAKLASPDALENALMKYYDVGN
                     ESISDILNDLATDDTLSLSEGRGESIDLETLREMAEANPVKRLVNMVLLEAIRNRASD
                     IHFEPFEDDFKMRYRIDGVLYEMLPPPKSIAVAIASRIKVMSNLDIAERRLPQDGRIE
                     LVVNGNPVDLRVAVLPTMFGESVVMRILDRSNVSLDLERLGIRDDDLRTFRQLIHRPH
                     GVIIVTGPTGSGKTTTLYSALNELNDISVKILTAEDPVEYDIDGLIQCQINEDVGLTF
                     ARCLRSFLRQDPDIVLVGEIRDLETAKIAVEASLTGHLVFTTLHTNDAPSSIARLVDL
                     GLETFLITATLEAIVAQRLVRRICPKCKEEFQPTDDMLMELDL"
     gene            complement(1499..2596)
                     /locus_tag="PLK08_08365"
     CDS             complement(1499..2596)
                     /locus_tag="PLK08_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPS53552.1"
                     /translation="MATLHIDRLLETCIKSGASDLHLCVGLPPMLRLRGRLRPLETKT
                     LTPEDTTELMKSITPERSQQELQEVGGTDFGFEFGDKGRFRTSVFMQKGHISLVLRLI
                     PNKLFSLDEIGLPEITRTLLRRPRGMFLVTGPTGSGKTTTLASMINYINENFDKHIIT
                     VEDPVEYYHPHKKSIVVQREVGVDVPSFSEALRRALRQDPDVMLVGELRDLETIEAAL
                     RAAETGHLVFGTLHTSSAQGTITRIVDQFPVDQQEQVRVQLAESLIAVLCQALMPRCD
                     VKGMVAAYEFMVVTSAISNMIRENKIFRIPSTIQTGKKLGMQLLDDHLYELFAAGKIS
                     EEDALDRSQTPADLLEKIKRLKATQSGAATK"
     gene            complement(2728..>3316)
                     /locus_tag="PLK08_08370"
     CDS             complement(2728..>3316)
                     /locus_tag="PLK08_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPS53553.1"
                     /translation="RSMLRQAPNIILVGEIRDLEVAEVAIQAALTGHLVFSTLHTNDA
                     PSALTRLIDMGVKPFLVASSVQAIMAQRLVRRICPDCKSVDESPNLRTLKLLGFRPEE
                     IENSTFYVGKGCNSCNGSGYRGRQGIFEIMELTSQIRQMTFERATLAEIRKAAKAAGM
                     KNLLEDGKMKIKNGITTPEELLRITQVEELVSEEE"
BASE COUNT          700 a          865 c          917 g          834 t
ORIGIN      
        1 aggtcgagtt ccatgagcat atcgtccgtc ggctggaatt cttccttgca tttcgggcat
       61 attcgccgca caagacgctg ggcgacgatt gcttcgagag tggcggttat aaggaatgtc
      121 tccagtccca ggtcgaccag acgcgcgatg cttgacggcg cgtcattggt gtgcagggtc
      181 gtgaacacca ggtggccggt aagcgaagcc tccaccgcga ttttggcggt ttcaaggtca
      241 cgaatttcgc cgacgagcac gatgtcgggg tcctgacgca ggaaacttcg caggcagcgg
      301 gcgaatgtaa ggccgacgtc ttcgttaatc tgacactgga ttaggccgtc gatgtcgtat
      361 tcgaccggat cttccgccgt caggattttt acggatatgt cgttaagttc gttcagggct
      421 gaataaagcg tcgtggtttt gcccgaacct gtcgggccgg tcacgattat cacgccgtgc
      481 ggccgatgaa tgagctgacg gaacgtccgc aggtcgtcgt cgcgaatgcc cagccgctca
      541 agatcgagcg aaacgttcga acggtcgaga atacgcatta cgacgctttc gccgaacatc
      601 gtcggaagaa cggcgacacg caggtcgact ggattgccgt ttacgacgag ttcaattcgg
      661 ccgtcctgcg gcagacgacg ctcggcgatg tccagatttg acatgacctt gatgcgcgac
      721 gctatggcta ccgcgatgga tttcggcggc ggcagcatct cgtacaatac gccgtctatg
      781 cggtatcgca ttttaaaatc gtcttcgaac ggttcgaagt gaatgtcgga ggcacggttg
      841 cggatggctt cgaggagaac catgttgacc agccgcttga cggggttggc ctccgccatt
      901 tcacgcaggg tttcaaggtc tatgctctcg cctctgcctt cagaaagact caatgtgtcg
      961 tctgtggcga ggtcgtttaa tatgtccgat atcgattcat tgccgacgtc gtaatatttc
     1021 atcaacgcat tttcaagggc atcgggcgag gccagcttgg ctgtcacgcc tgaaaagccc
     1081 atcagcgtct gaaggtcgtc gacggctctg aagttgttgg gcgtacagac ggctatcgtc
     1141 agatgattcg tatcggggtc gtgttccagc gggataatct tgtagctggt ggctacctgc
     1201 gcctggataa ggtttatgac cgattcgggg atatccatcg tcgtgaggtc gacgaactcc
     1261 atgccgacct gagcgcccag ggctaacgac agggtggcgt cgtcgatgaa gcccagctcc
     1321 acgagatgct gtcctatcgg gccgctttgc tgtttttgcc gttccagagc ctgataaacc
     1381 tgctctttgg ttattttgcc catcttgacg agaacacggc ccagcggcct gccccgcaac
     1441 tggtctatcg gaatgttgag tgcattcatg tttgccattt gacgactccg ttcattcatt
     1501 acttcgttgc tgcgccgctt tgggtagcct tgagacgctt gattttctca agcaggtcgg
     1561 ctggcgtctg tgacctgtcg agcgcgtctt cctcggaaat cttgccggcg gcgaacagtt
     1621 cgtaaaggtg atcgtcgagc agctgcatgc ccagcttttt gccggtctgt attgtcgatg
     1681 gaatacggaa tatcttgttt tcgcgaatca tgttcgatat tgcactggtg acgaccatga
     1741 attcgtaagc tgcgaccatg cctttcacgt cgcatcgcgg catgagagcc tggcagagta
     1801 cggcgatgag cgattccgcc aactgcacgc gaacctgttc ctgctggtcg acggggaact
     1861 ggtcgacgat acgcgtgatt gtgccctgag cggacgaagt atgcagagtg ccgaagacga
     1921 ggtggccggt ttcggcggct cgcagtgcgg cttcgatggt ttcaaggtcg cggagttcgc
     1981 cgacgagcat tacgtcgggg tcctgtcgca acgcacggcg aagggcttcg gaaaagctcg
     2041 gcacatccac gccgacctca cgctgaacca cgatggattt cttgtgcgga tggtaatatt
     2101 ccaccggatc ttcgacggtt atgatatgtt tgtcgaaatt ctcgtttatg taattaatca
     2161 ttgacgccag cgtggtggtt ttgccggaac cggtcggccc cgttaccagg aacatgccgc
     2221 gtggccgtcg cagcaacgta cgcgttatct ccggcagacc gatttcgtca agcgagaaga
     2281 gcttgttggg tatcagacgg agaaccagtg agatgtgacc tttctgcatg aacaccgacg
     2341 tacggaaacg gcctttgtcg ccgaattcga aaccgaagtc tgtgcccccg acttcctgca
     2401 gttcctgttg agaacgctca ggcgttatgg acttcatgag ctcagtcgta tcttcaggcg
     2461 taagagtttt cgtctccaac ggacgaagcc ggccacgaag acgaagcatc ggcggcagac
     2521 ccacacaaag atgcaagtca ctggcgccgc tcttgataca agtttccaac agtctgtcaa
     2581 tgtgcaatgt cgccatattt ttcatccacc tcttctgtca gcctaattat tcgttcccaa
     2641 ttacactcaa aagtaatacg gaaatttcaa acggctgcca ttttgttctt aatatattgt
     2701 taataaaacg ttatgaaaaa taaaaaatta ctcttcttca gagaccaatt cttcgacctg
     2761 ggtgattcgt agcagttctt ccggcgtggt aatgccgttt tttatcttca ttttgccatc
     2821 ttcgagcaga tttttcatgc ccgccgcctt tgccgccttg cgtatttccg caagcgttgc
     2881 gcgttcgaat gtcatctggc gaatctggct ggtcagttcc atgatttcga aaatgccctg
     2941 acggcctcgg tagcctgagc cgttgcagga attgcaacct ttgcctacat aaaatgttga
     3001 attttcaatc tcttccggcc ggaagccgag gagcttcagc gtacgcagat tgggcgattc
     3061 gtccacactt ttgcaatccg gacaaatgcg tcgcacaagc cgctgagcca taattgcctg
     3121 aaccgagctt gccacaagga acggtttgac gcccatatca ataagacgcg tcaaagccga
     3181 cggcgcatcg ttggtatgta gtgtactgaa aaccaggtgt cctgtcaaag cggcctgaat
     3241 tgccacttct gcgacttcaa ggtcacgaat ttcgccgacg agaatgatgt tcggcgcctg
     3301 acgtaacatt gacctg
//
LOCUS       DAONFI010000270         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_36079_length_3296_cov_3.641160, whole genome shotgun sequence.
ACCESSION   DAONFI010000270 DAONFI010000000
VERSION     DAONFI010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36079_length_3296_cov_3.641160"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLK08_08375"
     CDS             <1..697
                     /locus_tag="PLK08_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HPS53554.1"
                     /translation="RLTGLLLLLPLSLGLLAGCDGEISADKQEVAYLPYYELPSAHVT
                     NASFVQTLASHHVTAVKPNHIAKHSTSSASPWYVNGNRPWKYIVIHHSASPSGNSEKF
                     LAMHLARGWDEMGYHFVITNGNGGPDGAVEIGSRWLKQKWGAHTGGTPGNEYNNFGIG
                     VCLVGNFDETRPTPAQLHSLEQLVRFLVKTYNIDTDNIIAHKDAPNAHTRCCGQSLYT
                     YIHSQEFKQKVAK"
     gene            694..1032
                     /locus_tag="PLK08_08380"
     CDS             694..1032
                     /locus_tag="PLK08_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPS53555.1"
                     /translation="MTASCRTNLFDVDGMSMNMPQEFLQILAEGKNVRIERIVSASHS
                     SPKNFWYDQQQSEWVAVLAGQAEIEFANGNISAMSAGDAILIPAHQRHRVKSTAADRP
                     TIWLAVFFEE"
     gene            1397..3052
                     /gene="lysS"
                     /locus_tag="PLK08_08385"
     CDS             1397..3052
                     /gene="lysS"
                     /locus_tag="PLK08_08385"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPS53556.1"
                     /translation="MITKVQADRQAKLEKLRDMGVDPYGWRYDDVERATSIQNRYSDD
                     APEGSQHARTAGRITLLRDMGKMMFITLRDSTGHIQTALRKDALGEQPWEVARLLDLG
                     DIIGVEGMLQRTKTGEITIWADKLKLLSKSLLPMPEKFHGLTDLEMRYRQRYLDLMTN
                     PESMQNFKIRIAIIEKIRSLLKARGYTEVETPMLQPIYGGAAARPFTTHHNALDMLLY
                     MRISPELYLKRLLVGGMEKVFEINRNFRNEGLSTRHNPEFTMMELYEAYADYNVMMEI
                     AEELFTTIADEICRPRLIAQRDARAAEIEQIKIDAERMLANKRIPLSDEQRKAKYDEA
                     VARYEAEKIAFPKTPLYKAAAACADGNLVLPYGDILLDFKKPWRRAKYADLLMEYANV
                     NINDIAAVRSKARELKIDEARLDDVVVVNEVFEATVEEHLVQPTFVCDYPAALCPLTR
                     RDPNNPDIALRFELFAAKMELANAYTELNDPKIQEDNLAGQIKGEEEETMRVMDEDFV
                     TALKHGMPPAGGMGIGIDRMVMLLTDTQSIRDVILFPLLKPEA"
     gene            3113..>3296
                     /locus_tag="PLK08_08390"
     CDS             3113..>3296
                     /locus_tag="PLK08_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPS53557.1"
                     /translation="MAKPLMLLIRDGWGIGNGGPGDAVAAAKTPNMDALLAKYPTCTL
                     DASGISVGVRAGSQGSS"
BASE COUNT          877 a          887 c          842 g          690 t
ORIGIN      
        1 gcgattgacc ggacttttgt tgttgctgcc gctctctttg ggcctgctgg ctggatgcga
       61 cggagaaatt tccgcagata aacaggaagt agcctatctg ccgtattacg aacttccgtc
      121 agctcacgtc accaacgcct ctttcgtgca gactctcgcc agccatcacg tcaccgcagt
      181 caagccgaac catatcgcca aacattcgac ctcgtcggcc agcccgtggt acgtcaacgg
      241 caacaggccg tggaaatata tcgtcatcca tcattccgca tcgccgtcgg gcaattcgga
      301 aaaatttctg gctatgcacc tcgctcgcgg ctgggatgag atgggatatc atttcgtcat
      361 aacgaacggt aacggcgggc cggacggagc ggtggaaatc ggcagccgat ggctgaaaca
      421 gaaatggggc gcacataccg gcggtacgcc gggcaacgaa tataacaact tcggcatcgg
      481 cgtatgtctc gtagggaatt tcgacgaaac acgtcctacg ccggcacaac ttcacagcct
      541 tgaacaactc gtccgctttc tcgtcaaaac atataacatc gacactgata acatcatcgc
      601 gcacaaggac gcccccaacg cacacacccg ctgctgcggc caaagcctct atacttacat
      661 ccattcgcag gaattcaaac aaaaggtcgc caaatgaccg catcatgcag gacgaatctg
      721 tttgacgtcg acggcatgag catgaatatg ccgcaggaat ttctgcagat tttggctgaa
      781 ggcaaaaacg tgcggataga acgtatagtg tcagccagcc attcgtcgcc caaaaacttc
      841 tggtatgacc agcagcagtc tgaatgggtg gctgtgctcg cgggacaagc cgaaatcgaa
      901 tttgccaacg gtaatatatc tgccatgtca gccggcgatg cgatactcat ccccgcacat
      961 caacgccatc gtgtaaaatc aacggcggcg gacagaccaa caatctggtt ggccgtcttt
     1021 tttgaagaat gaaaaaatca gcctcgccgg ccttgcatct tttctcaaaa atcattgact
     1081 ttcgggattg ttttggggat tatctctaga gcattgcaaa gttgagagtt cctatttttg
     1141 cgctagtggg tttgttcgtg gcaaggaagg aaaatgaggt atatttgatg atattcctaa
     1201 aaaaattctg acacagccac gaacaaagca aaccagcaaa aaaggaagtc gcaactttaa
     1261 gatgctctaa ttccgatttt tgaaatttca aaaatcgcag gcaaaacagg ccaattctgt
     1321 atgcaaataa ccctacaggt taaaatccga cggttaaaat ctgcggcaga cggctgataa
     1381 gtgattggaa acaaagatga taacaaaagt acaggctgac aggcaggcta aactggaaaa
     1441 gctccgcgat atgggcgttg acccctatgg ctggcggtat gatgacgttg aacgtgcaac
     1501 aagcatccag aatcgttaca gtgacgacgc tcccgaaggt tcgcaacacg cccgcaccgc
     1561 cggacgtata accctgctgc gcgacatggg caaaatgatg tttataacgc tgcgtgattc
     1621 caccggccat atccagactg ctctgcgcaa ggatgcactc ggcgagcagc cgtgggaagt
     1681 cgcaagatta ctcgatttgg gcgatatcat cggcgtggaa ggcatgcttc aacgtacgaa
     1741 aaccggcgaa ataaccatct gggccgacaa attgaaactg ctctccaagt cgcttctgcc
     1801 gatgccggaa aaattccacg gcttgacgga cctggaaatg cgttaccgtc agcgttatct
     1861 cgacctgatg acgaatcccg aatcaatgca gaatttcaag attcgcatcg ccataataga
     1921 aaaaatacgc tcgctgctca aggctcgcgg ctacacggaa gtggagacgc ccatgcttca
     1981 gccgatttac ggcggtgcgg cggcgcggcc gtttacgacg catcacaacg cgctggacat
     2041 gctgctgtat atgcggataa gtcctgagct ttatctcaaa cgactgctcg tgggcggcat
     2101 ggaaaaggtt tttgaaatca atcgcaattt ccgcaacgaa ggtttaagta cgcggcacaa
     2161 ccctgagttc accatgatgg aactctacga agcctacgcc gactacaacg tgatgatgga
     2221 aatcgcggag gaacttttca cgacgattgc cgatgaaatc tgtcgtcccc gtcttatcgc
     2281 ccagcgcgac gcccgcgcgg cggaaatcga acagataaaa atcgacgccg aacgcatgtt
     2341 ggctaacaag cgtatccccc tgagcgacga gcagcgcaag gctaagtacg atgaagccgt
     2401 cgcccgttac gaagcggaaa aaatcgcatt cccgaaaact ccgctctaca aagctgccgc
     2461 cgcctgtgcc gacggcaacc tcgtcctgcc gtatggcgat atactgctcg acttcaaaaa
     2521 accgtggcgt cgcgccaagt acgccgacct gctcatggaa tacgccaatg tgaacataaa
     2581 cgacattgcc gcagtccgca gcaaggcacg cgaattgaaa atcgacgagg cccggcttga
     2641 cgacgtcgta gtcgtaaacg aagtttttga agcaaccgtc gaagaacacc tggttcagcc
     2701 gactttcgta tgcgattatc cggctgccct ctgcccgctt actcgtcgcg acccgaataa
     2761 tcccgacatc gccctgcggt tcgaattgtt cgccgcgaaa atggagctgg cgaacgcata
     2821 taccgaattg aacgacccga aaattcagga agacaacctc gccggccaga ttaagggcga
     2881 ggaagaggaa accatgcgtg taatggatga agatttcgtt acggcgctca agcacggcat
     2941 gccgccagcc ggcggcatgg gcattggcat tgaccgcatg gtcatgctgc tgaccgatac
     3001 gcaatccata cgcgacgtca tattgtttcc cctgctgaaa ccggaagcgt aacgaaaccc
     3061 attttgattt tagttcactg acacagtcag gcttgaaagg atttttgcaa aaatggccaa
     3121 acctttgatg ttattgattc gtgacgggtg gggcatcggc aacggcggcc ctggtgacgc
     3181 ggtggctgcg gcgaagacgc cgaatatgga cgcgctcctc gcaaaatacc ccacatgcac
     3241 gctcgacgct tcgggcatct ccgtcggcgt tcgtgcaggc tcgcagggtt caagcg
//
LOCUS       DAONFI010000271         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_36253_length_3284_cov_4.567978, whole genome shotgun sequence.
ACCESSION   DAONFI010000271 DAONFI010000000
VERSION     DAONFI010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36253_length_3284_cov_4.567978"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1784
                     /locus_tag="PLK08_08395"
     CDS             63..1784
                     /locus_tag="PLK08_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPS53558.1"
                     /translation="MITMEINGKTVEAHEGETILKVLRREGINVPTLCYMDGLTPTGA
                     CRMCVVEVEGARGLVPSCAFPVGNGMKIQTHSPRAIRARKTIIELLLADHPDDCLFCV
                     RNGTCQLQSLASELGVRERRFQGEKSVFPQDISSPSIVRDPEKCILCGKCVRMCEEVQ
                     GVSAIDFINRGSKAIVGTAFNQGMNCSTCINCGQCIQVCPTGALREHSDLKKVSEALA
                     DPEKVVIVQHAPAVSVTLGEEFGCKAGTDVMGLMTAAFRKLGFSRVFDTSFSADLTIM
                     EEGSEFVKRLTAGGTLPMFTSCSPGWIKFVETFYPEFIGNLSTCKSPQQMLGAIIKSY
                     YADKEGIDPKNIFSVSVMPCTAKKFEARRMEMGREGIFDIDAVLTTRELARLIRMRHI
                     DFNKLQPEVADNPFGERTTAGKLFGASGGVMEAAIRSAYFLVTGRELEELKVEAVRGL
                     DGVKEASIDIDGTKVNVAVVSGLANARKLLEEIKAGRKDLHFIEVMTCPGGCINGGGQ
                     PYGTNLDSVQQRMKALYRIDKDEPVRVSHKNKSVAKLYEDFLGKPLGEKSHHLLHTHY
                     HVRDEVM"
     gene            1861..2265
                     /locus_tag="PLK08_08400"
     CDS             1861..2265
                     /locus_tag="PLK08_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPS53559.1"
                     /translation="MIDFKIKLPCRILVIDDDPDFLAQMKMQLGDNGIDVITADSSAE
                     AGRLLAEETFDMAIVDIMMEEEDSGFALCYQIKKHNPDMPVIIATSVASKTGIVFDAI
                     TAEERKWIKADVILDKPFREEQLLREIERLRR"
     gene            2508..3245
                     /locus_tag="PLK08_08405"
     CDS             2508..3245
                     /locus_tag="PLK08_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPS53560.1"
                     /translation="MNDNFEKNGGDLNIGLAHMMAHELKSPLSVIEGYLYILKKKSRA
                     DDFNPQDLQVILDRCTARLEQIRHVIDDLVALDCADRSRDETLRTDVAKVALDCVEQI
                     QETADKSRVKISFDVSPAEVAATPGELSMIISNLLCNAVKYNRPEGKVSLTVKPEDNS
                     VLLEVGDDGLGIAPENISKIFKPLWREKTPAKRDIPGTGVGLSIVEKMVKILGGVISV
                     TSTQNEGTMFRIVLPKAAGCNGIKESN"
BASE COUNT          847 a          830 c          909 g          698 t
ORIGIN      
        1 cttacgacgt gtcgtttcgg cgcagttgta aaagagtaac ccgaaaacag gacgtataaa
       61 acatgataac aatggaaata aacggcaaaa cggttgaggc ccacgagggc gaaaccatat
      121 tgaaagtgct tcgccgcgaa ggcatcaacg ttccgacgct gtgctacatg gacggcctta
      181 cgcctaccgg cgcatgccgc atgtgcgtag ttgaggtcga gggcgccaga ggccttgtgc
      241 cgagctgtgc ctttccggtc ggcaacggca tgaaaatcca gacccattcg ccgcgagcca
      301 tccgtgcacg caaaaccata atagaactgc tgctggctga ccatccggac gactgcctgt
      361 tttgtgtccg caacggcaca tgccagttgc agagcctcgc cagcgagctc ggcgttcgtg
      421 aacgtcgttt ccagggcgaa aagagcgtct tcccgcagga catttccagt ccgagcattg
      481 tacgcgaccc cgaaaagtgc attctctgcg gcaaatgcgt gaggatgtgc gaagaggttc
      541 agggcgtatc cgcgatagat ttcatcaatc gcggcagcaa ggcgattgtg ggcactgcct
      601 tcaatcaggg catgaactgc tccacctgca tcaactgcgg ccaatgcatc caggtctgtc
      661 cgactggcgc tctgcgcgag catagcgacc tgaagaaagt ctccgaagcg ctggctgacc
      721 cggaaaaggt cgtcatagta cagcacgcgc cggcggtgtc ggtcacgctt ggcgaagagt
      781 tcggctgcaa ggccggcact gacgttatgg gcctgatgac ggcagcgttc cgcaaactgg
      841 gattttccag ggtgttcgat accagctttt ctgccgacct cacgataatg gaggaaggct
      901 cggaattcgt caaacggctg accgccggcg gcacgctgcc gatgttcacc agctgctcgc
      961 cgggctggat aaaattcgtc gaaacttttt atcccgaatt catcggcaat ctttcgacct
     1021 gcaagagtcc gcagcagatg ctcggcgcaa tcatcaaatc gtactatgcc gacaaggaag
     1081 gcatcgaccc gaaaaatatt ttcagcgttt cggtcatgcc ctgcaccgcc aagaagtttg
     1141 aagcccgtcg catggaaatg ggtcgcgaag gcatatttga tatcgacgca gttctaacca
     1201 ctcgtgaact ggcgcggctg atacgcatgc ggcatatcga cttcaacaaa cttcagccgg
     1261 aagtggcgga caatccgttc ggcgaacgta ccacagccgg caaacttttc ggcgcgtccg
     1321 gcggcgtaat ggaagcagcc atccgcagtg cgtacttcct cgtaaccggc agggaactcg
     1381 aagaacttaa ggttgaggcg gtgcgaggcc ttgacggcgt gaaagaagca agcatcgata
     1441 tcgacggcac aaaagtcaac gtagccgttg tcagcggctt ggcgaacgca cgcaaactgc
     1501 tcgaagaaat caaggccggc cgcaaagacc tgcatttcat cgaagtgatg acctgccctg
     1561 gcggctgtat caacggcggc ggccagccgt acggcacgaa cctggattcc gtccagcagc
     1621 gaatgaaagc cctctatcgt atagacaagg acgaacccgt cagagtctcg cacaagaaca
     1681 agtccgtggc gaaactttac gaagatttcc tgggcaagcc gcttggcgaa aagtcgcacc
     1741 acctcctcca cacgcattac catgtgcgcg acgaggtcat gtgatacagt gtgttaaccg
     1801 caacccccgc atgcgtgcct gtgaaacagc ccgcatagag gattttggag attttttaac
     1861 atgatagatt ttaagataaa attgccgtgc aggattttgg tgatagacga cgacccggac
     1921 ttcctggcac agatgaagat gcagctcggc gataacggaa tcgacgttat aaccgctgat
     1981 agcagcgcgg aagccggccg ccttcttgcg gaagaaactt tcgacatggc aattgtggac
     2041 ataatgatgg aagaggaaga cagcggcttt gccctgtgct accagattaa aaagcacaat
     2101 cccgatatgc ccgtcatcat tgccacgagc gttgccagca aaaccggcat agtattcgac
     2161 gccataacgg ctgaagaacg caagtggata aaggctgacg ttatactcga caagcctttc
     2221 cgcgaagagc agcttttgcg ggaaatagag cgtctgcgtc ggtaacgggc agggtattgc
     2281 agaaggcggc tgcaggattt tttttgtaaa aaaacttgcg gacgacttga attgctggaa
     2341 tactggaaac ctgtttcaaa gactgctttc gtggtgaagc tggaatattt attacggctt
     2401 aaccatgcag ggcggcatat tggaacatat gtcttgacgc aggctggctg cgaaacgcca
     2461 gccgtagcgt aatagaggtt tgacaatttc cgttgaccgg tgattcaatg aacgataatt
     2521 ttgaaaaaaa cggcggcgat ttgaacatag ggctggcaca catgatggcc catgaactca
     2581 aatcgccgct gtccgttata gaggggtatc tctacatact taaaaaaaag tcccgggcgg
     2641 atgatttcaa tccgcaggac ttacaggtca tactcgaccg ctgtacggcg agacttgaac
     2701 agattcgaca tgtcattgac gaccttgtcg ctctggattg tgcggatcgt tccagggatg
     2761 aaactctgcg aaccgatgtg gcgaaagttg cgctggactg cgtcgagcag attcaggaaa
     2821 cggctgacaa atcgcgcgtt aaaatctctt tcgacgtatc gccggctgaa gttgccgcca
     2881 cgcctggcga actttcaatg ataatatcga atctgctttg caatgcggtg aaatacaatc
     2941 gtccggaagg gaaagtttcg cttactgtca agccggaaga taatagtgtg cttctcgaag
     3001 tcggcgacga cggtttgggg attgcgcctg aaaacatttc aaagattttc aaaccgttgt
     3061 ggcgtgaaaa gacgcccgca aaacgcgaca taccaggtac aggagtaggc ttgtcgatag
     3121 tcgaaaaaat ggtcaaaatc ctcggcggag taatctccgt caccagcacg cagaacgaag
     3181 gaacgatgtt tcgcatagtc ctgccgaagg cggcgggatg taacgggatt aaagagtcaa
     3241 attagagcag ttcaatcttg agagttcctt ttttgctggt ttgc
//
LOCUS       DAONFI010000272         3280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_36313_length_3280_cov_4.066977, whole genome shotgun sequence.
ACCESSION   DAONFI010000272 DAONFI010000000
VERSION     DAONFI010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36313_length_3280_cov_4.066977"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLK08_08410"
     CDS             <1..858
                     /locus_tag="PLK08_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPS53561.1"
                     /translation="SGNIIARMWQASETVFNGIPTDGFLHVKGRVEDYRGMPQMIIEA
                     CRPVSADKVDVSEFIPTSPYDVDEMWGEITEILREIKNTPLKLLVKKILEDKKLVTAI
                     KRAPAAVQMHHPYAGGLVEHTRNVMRNAVVLLPFYPQLSVDLVLAGTFLHDLGKSAEL
                     SSGINIFYTDRGQLIGHLTIAVVWIQEKAKLVEEETSQKFPHETITLLQHIVLSHHGE
                     YEYGSPKLPAIPEAFFIHFLDNLDAKMWMTENAIESDPDNESRWTTYLKQLDTKIFKP
                     HKELPEMES"
     gene            878..1690
                     /locus_tag="PLK08_08415"
     CDS             878..1690
                     /locus_tag="PLK08_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPS53562.1"
                     /translation="MSLTDWLSPKRRRRKKILAKPFPQEWLAIIEKNVPYYRHLDPDE
                     QDELQRHVQIFVAERHFIGCGGLDINDEIRVTIAAQACVLLLHRDREYRSSLKTIFVY
                     PHHYIARGLQQTGDGLFLEGPSIRLGESWHRGPVVLSWDDVLKNAADVHDGHNVVFHE
                     FAHQLDDENGSADGAPPLPKRSMYLAWARVLGHDYEQLVENVKHHHKTFIDAYGATNP
                     AEFFAVASETFFEKPIQLRHKNPDLYEQLKICYNQDPAGREMRHKNHHENNN"
     gene            1674..2591
                     /locus_tag="PLK08_08420"
     CDS             1674..2591
                     /locus_tag="PLK08_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HPS53563.1"
                     /translation="MKTIIEFLLCGVIVCIATNLLHGVFHRHETARESDVGSVLYAQI
                     ATPVFNTPDVRAVFGGKDGNTLKVDNQGLIRAVETVLLPGQSLRVRGETTINGIKMYR
                     VTSAAYPYPAKDGLYVDARFVGRQKPYCDLSMPANDKIISNMLSMVGSEYIWGGNYHD
                     GIMQMLQFYPPQNPLDGVQSSRWTLAGVDCSGLLYEATNGNTPRNTSSLITFGRPVGI
                     AGLSAKDIAARLKPLDLIVWKGHVLIAIDSGNIIESTMAPGPGGETGVRVTDAVTRLS
                     QIMHTRTPADSFSHAGQFVIRRWYGVDDK"
     gene            2982..>3280
                     /locus_tag="PLK08_08425"
     CDS             2982..>3280
                     /locus_tag="PLK08_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53564.1"
                     /translation="MKRMLWLFIMLLGISTVLMADTLSPSAGTLNATGYSSSLSTYTN
                     ALSVNFNMASAGNALVFSTFSCKTTGNSTTGTWQLSGSSGSSQAVQRYLSGSNDAG"
BASE COUNT          866 a          842 c          847 g          725 t
ORIGIN      
        1 tccggcaata ttatcgcgcg catgtggcag gcgtcagaga cggtgtttaa cggcattccg
       61 acggacgggt ttctgcacgt caagggcagg gtggaagatt atcgcggcat gccgcagatg
      121 ataatcgagg cctgccggcc cgtttcagcc gacaaggtgg acgtgtcgga attcattccc
      181 acctcgccgt acgatgtgga tgagatgtgg ggcgaaataa cggaaatcct tcgcgagata
      241 aaaaatacgc cgctcaaact gctggtaaag aaaatcctcg aagacaaaaa actagtcaca
      301 gccatcaagc gcgcgccggc ggctgtccag atgcatcacc cctacgccgg cggcctcgta
      361 gaacatacgc gaaacgtcat gcgcaacgcc gtagttttac tgccattcta tccgcagctc
      421 agcgtcgacc tcgtgctggc tggtacgttt ttacacgacc tgggaaaatc cgccgaactc
      481 tccagcggga taaacatttt ctacaccgac cgcggccagc tcatcggcca tctgacgata
      541 gccgtcgtat ggatacagga aaaagcgaag ctggtcgaag aggaaacgtc tcaaaaattt
      601 ccgcacgaga cgattacgct gttgcagcat atcgttctgt cgcaccacgg cgaatatgaa
      661 tacggcagtc cgaaactgcc cgcgatacct gaagcctttt tcattcactt cctggacaat
      721 ctcgacgcaa aaatgtggat gactgaaaac gcaatagagt ccgaccctga caatgaaagc
      781 cgctggacga cttacctcaa acagctcgac actaaaattt tcaagccgca caaggaactc
      841 cccgaaatgg agtcgtaaaa aacggagcat gaaattcatg tcgctgacag actggcttag
      901 cccgaaacga cggcgtcgca aaaaaatcct ggccaagccg ttcccgcagg aatggcttgc
      961 tataatagaa aaaaatgtgc cttattaccg tcatctcgac ccagacgagc aggatgaact
     1021 ccaacgtcat gtacagatat tcgtcgccga gcgtcatttc atcggctgcg gcgggctgga
     1081 cataaacgac gaaatccgcg tgaccatcgc ggctcaggca tgtgtgctgc tgttgcatcg
     1141 cgacagggag tatcgcagtt cgctgaaaac catcttcgtt tatccgcacc attacattgc
     1201 ccgcggctta cagcaaactg gcgatggact gttccttgaa gggccgtcga tacgtctggg
     1261 cgagagctgg catcgcgggc cggtggtgtt aagctgggat gacgtcctca agaacgccgc
     1321 cgacgtgcac gacggccata atgtcgtatt tcacgagttc gcgcatcagc tcgatgacga
     1381 aaacgggtcg gctgacggcg caccgccgtt gcccaaacgc tcaatgtatc tggcatgggc
     1441 ccgcgtgctc ggccatgatt acgagcagct tgtagaaaat gtaaaacatc accataagac
     1501 gtttatcgat gcatatggcg cgacgaaccc ggctgagttt ttcgcagtag ccagcgaaac
     1561 tttttttgaa aaacccatcc agctcaggca caagaatccg gatttgtacg agcagttgaa
     1621 aatctgttac aatcaggacc ccgccgggcg ggagatgcgg cacaagaatc accatgaaaa
     1681 caataattga atttctgttg tgcggcgtaa tcgtttgcat agcgacgaat cttcttcatg
     1741 gagtgtttca tcggcatgag acagcccgtg aatcagacgt cggcagcgtt ttatatgcac
     1801 agattgcgac gcccgtgttc aacacgccgg acgtgcgtgc cgttttcggc gggaaggacg
     1861 gcaatacgct gaaggtcgac aatcaggggc tcatccgcgc ggtggagaca gtactgctgc
     1921 caggccaatc gctgcgcgtg cgcggcgaga cgacaatcaa cggcatcaag atgtaccgcg
     1981 tgacgtctgc cgcctatccg tatccggcaa aggacggcct ttatgttgac gcgcgtttcg
     2041 tcggccgaca aaaaccgtat tgcgacctgt ccatgccggc taatgacaag ataatttcga
     2101 acatgctttc gatggtcggc agcgaataca tctggggcgg caactatcac gacggcataa
     2161 tgcagatgct gcaattctat ccgccacaaa acccgctcga cggcgtacag tcgtccagat
     2221 ggacgctcgc aggcgtagac tgttccggcc tgctctatga agcaaccaac ggcaatactc
     2281 cacgcaacac gtccagcctc ataaccttcg gccggccggt cgggatagcc ggtctttctg
     2341 ccaaagacat cgcagccaga cttaagcctc tcgacctgat agtgtggaaa ggccatgtcc
     2401 tcatagccat agatagcggt aatattatcg aaagtaccat ggccccaggc cctggcggcg
     2461 aaacaggcgt tcgcgtaacc gacgccgtca cacgattatc acaaataatg cacacacgca
     2521 cgccagccga cagtttctcc cacgccggac aatttgtcat cagacgatgg tacggagtgg
     2581 atgacaagta aaatactccc gaaaaagaaa gttttgtctg atataaaaat acggtatttg
     2641 ccctataaac atcaaaaaaa gggctgtttt tggaaaaatt ctgattgaca gacctgttat
     2701 tcggtttatc attatgaatg tttgagagcg ccggaggtag cctccgacaa atcgtccaat
     2761 agagcttggc gataagatac gccgctacaa ttcggaattg agggattatt tacttactta
     2821 tttgacgttt tgcataagaa gtaaagttgt ctttatgcca aagggtaaac atttagggtg
     2881 aacggaatcc ggcgaattgt gtggataacg tggctgtggg aaattgaggt cggctgttgg
     2941 ttttgaaccg acgagatatt caatgacaaa ggagaattct tatgaaacgc atgctatggc
     3001 tttttattat gttgcttggg ataagcacag tgcttatggc tgatacgttg tcacccagtg
     3061 cagggacttt aaatgctaca ggatattcaa gcagtctgtc aacttatacc aatgcgcttt
     3121 cggtaaattt caatatggcc agcgccggca atgcgctggt cttcagcaca ttttcatgca
     3181 agacaacagg taattcaaca accggcacat ggcaattatc gggttcaagc ggttcttctc
     3241 aggcagtcca gagatatctt tccggcagta acgacgcggg
//
LOCUS       DAONFI010000273         3270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_36483_length_3270_cov_4.524417, whole genome shotgun sequence.
ACCESSION   DAONFI010000273 DAONFI010000000
VERSION     DAONFI010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36483_length_3270_cov_4.524417"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1205)
                     /locus_tag="PLK08_08430"
     CDS             complement(210..1205)
                     /locus_tag="PLK08_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS53565.1"
                     /translation="MSVYVSLTPHCPFRCRHCSVTSRRPSEMTHELTLDVLSQIRRLG
                     ACIVGFTGGEPLLRKDLPELIASLQGDVTSMVFTTGWGLTPDLARQLKAAGLDCLTIA
                     IESASPKTHDAARNFEGSFQAAELAIGVARDAGLYTAVSTVGTHAKISSGELEKIYDK
                     TRQWGVQEFRLVASVATGEKAGCPSFMLRPEEHEKLANFHRDNNAAKAGGPTVASSAY
                     LESDEFFGCGAGYNHLFIDSAGEVCPCDFMPLSFGNCTQKPLAEIWKEMNAYFPRPRC
                     GCIMNRLAGKIKPDSILPLPVSQSQTLISPPARNAPLPQGYLRFLKKDVARYGRV"
     gene            complement(1769..2941)
                     /locus_tag="PLK08_08435"
     CDS             complement(1769..2941)
                     /locus_tag="PLK08_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPS53566.1"
                     /translation="MNEVRIGVIGCGGRGAVAENAVLPGVGGRLVAGADILDSALKQF
                     QEKYGADTFVTKDYRKLLERKDIDAVFVTSPDFMHEEHAMAALDSGRHVYLEKPMTIT
                     IEGCDRLLRKAFEKKLKLYLGHNMRHMSFVQKMKNLIDDGAIGQVKAGWCRHFVAYGC
                     DAYFKDWHADRSRSTSLLLQKGAHDIDVLHWLCGGYTVRTTAIGNLSVYNQITDRHSP
                     KERGDASWSDDNWPPLSQKGLNPIINVEDISMMLMNLDNGVLCSYQQCHYTPDAWRNY
                     TIIGTEGRIENFNDAPGDCVVRLWNKRKSYNPYGDQQFFIPGVNGTHGGADSSIVAEF
                     IRYIREGGKIKTSAIAARNSVAAGCCAAESLRSGSIPINIPPIPVEVKEYFTKDVE"
     gene            3037..>3270
                     /locus_tag="PLK08_08440"
     CDS             3037..>3270
                     /locus_tag="PLK08_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53567.1"
                     /translation="MRKKFNNICTNPLSETMDSLADSLFNNPLPYTNDVLPTAKNRQP
                     NRVLIFSHKQGFRHNATSYHERFVLVLMLRGSGT"
BASE COUNT          895 a          884 c          685 g          806 t
ORIGIN      
        1 cgctttcagc gacaaggcct tcgacctgcc gatgaatcga aattatctgc cgccgcttgg
       61 cacttccatt cgtatttctc gcgtcaaaac tttcatgccg acaaatccct tcacaatacc
      121 aagacactct ggcattctag ttttgccgcc gacgttctac aagtcaagtt taatctgaaa
      181 tacgctgaaa atgttcagat ttcatgcctt cagacacggc catagcgagc gacatccttt
      241 ttcagaaacc gtaaatatcc ctgtggtaac ggtgcatttc tcgcaggagg gctaatgagg
      301 gtttgagatt gcgaaacagg caacggcaat atggaatcag gtttgatttt accggctaac
      361 ctgttcatga tacagccgca acgcggccgt ggaaaatatg cgttcatttc tttccatatt
      421 tcagcgagcg gcttttgagt acaattgccg aatgacagcg gcataaagtc gcacgggcag
      481 acttctcctg cggaatcgat gaaaagatga ttatatccgg cgccacagcc gaaaaactca
      541 tccgattcca ggtaagccga acttgccacc gtcggcccgc cggctttggc ggcattgttg
      601 tcacgatgga aattagcgag cttttcgtgc tcttcaggtc ggagcatgaa cgagggacag
      661 ccggcttttt ccccagtcgc aacagaagca acaagacgaa actcctgcac gccccattgt
      721 cgggttttat cgtaaatttt ctccaactca cctgaagata ttttggcatg cgtaccgaca
      781 gtcgaaactg ccgtgtaaag gccggcatca cgcgcaacac cgatggctaa ctcagccgcc
      841 tggaacgatc cctcaaaatt tctggcagcg tcgtgagttt tcggagatgc cgactcgata
      901 gcgatagtca gacaatcaag gccggcagcc ttcaactgcc tggcaagatc gggcgtaagc
      961 ccccaaccag tcgtaaagac catgctcgta acatcgccct gcaatgaagc tataagttcc
     1021 ggcaaatctt tacgcaataa cggttcaccg ccggtaaacc ctacaataca cgccccaagc
     1081 cttcgtatct gggaaagcac atccaacgta agttcatgag tcatctcgga agggcgtcgg
     1141 ctggtaacgg aacagtgcct gcacctgaac ggacagtgcg gcgtaagaga aacatatacg
     1201 ctcaacggca cacgtcgtct attggcaaca tgatcaaaaa aacgactgaa tgcagggctt
     1261 ggccatggcg ggaaaaaact attgatacgt acctgagagt caaatctgtg cagcttttcg
     1321 cattgcattt tccgccaaaa ataccataaa tgccccatcg gcacccttcc gacaagtccg
     1381 ccgaacattt ttacaagttc gaacattttt acaagtacac ctccttcatt ctataattcg
     1441 ccaaaaatga cacgacccca taaaaatcaa caaaaagccc cgattaaaaa gcaagaaaac
     1501 agactcaaga ctccattttc tttaaaatat atcgggaaat atggctagaa caaaaaaaat
     1561 gacgcccgtt acttccccgt cgcgctgtat tataaccatt cacatataag gatgcaaaat
     1621 attacggcta actatatatg aaaaagtgtc actacgaaca gtggcacttc catcaggtct
     1681 ttatattatt tcagcagcaa aacataccct atagccgaca atcatcgcag ttctgccgcc
     1741 tgattcattc gcaaaacact cgttcatttc actccacgtc ttttgtaaaa tattctttca
     1801 cttctaccgg tatcggcgga atatttatgg gaatcgagcc actacgcagc gattcggcgg
     1861 cacagcagcc ggcggcaacg gagttacgtg cagcgatagc cgacgtcttt atttttccgc
     1921 cttccctaat ataccttatg aattcggcaa ctatactgga atcagccccg ccgtgagtgc
     1981 cgttcacacc tggaatgaaa aactgctggt cgccatatgg attgtacgac ttgcgtttgt
     2041 tccacagcct tacaacgcaa tcgccaggtg catcattaaa attctcaata cggccttcag
     2101 tgccgattat tgtataattc cgccatgcgt caggcgtata atgacactgc tgatagctgc
     2161 ataaaacgcc attatccaga ttcatcagca tcatgctgat atcttctaca tttataatcg
     2221 gatttaaacc tttctggctg agcggcggcc agttgtcatc gctccagctt gcatcgccgc
     2281 gttctttcgg cgaatgccgg tcagttatct ggttatatac gctgagattg ccgatagccg
     2341 tcgtacgtac cgtatatccg ccgcacaacc agtgcagtac gtcaatatca tgcgcccctt
     2401 tctgcaaaag cagcgaagtc gacctggagc ggtcggcgtg ccagtccttg aaataggcat
     2461 cacagccgta ggcgacgaaa tgcctgcacc agccggcttt gacctggccg attgcaccgt
     2521 cgtcgataag atttttcatc ttctgtacaa acgacatgtg ccgcatattg tggccgagat
     2581 acagcttgag ttttttctcg aacgcttttc tgagcagccg gtcacagcct tctattgtaa
     2641 ttgtcatcgg cttttcaaga tagacatgcc tgcccgaatc cagagcagcc atagcgtgtt
     2701 cctcatgcat gaaatcggga ctcgtcacaa aaacagcatc tatatccttc cgctccagca
     2761 gcttccgata gtctttcgta acaaacgtat cggcgccgta tttttcctgg aactgtttca
     2821 aagctgaatc aagtatatct gcaccagcga ccagtctgcc gcccacgcct ggcaaaaccg
     2881 cattctcggc tacagcgccc cggccgccac agcctataac acctatgcga acttcattca
     2941 tggcaaaact cctcaaaaaa tttcatcaaa ggcaaaactc cgtcctctat ctgaatattt
     3001 atgttataat gagtctaaat aataagaaat cacattatgc gtaaaaaatt taacaatata
     3061 tgcactaatc cgctgtcgga aacaatggac agccttgccg acagcctatt caacaatccc
     3121 ctgccgtata caaacgacgt tctgccgacg gcaaaaaatc gccagccaaa ccgcgttctg
     3181 atattctcgc ataaacaggg ctttcgtcac aacgccacaa gttatcacga aagattcgtg
     3241 ctggttctga tgttgcgcgg cagcggtacg
//
LOCUS       DAONFI010000274         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_36511_length_3268_cov_5.286959, whole genome shotgun sequence.
ACCESSION   DAONFI010000274 DAONFI010000000
VERSION     DAONFI010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36511_length_3268_cov_5.286959"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PLK08_08445"
     CDS             complement(<1..1085)
                     /locus_tag="PLK08_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPS53568.1"
                     /translation="MSKLMLINTIEGHECRIAVLAENKLQELYSERASAASQVGNIYK
                     GRVTNIEPSIQAAFIDYGGPKNGFLHISDLHPKYFPTPSRQLESVGRRSSHKSRPPMQ
                     ECLRPGQEVVVQMTKEGIGTKGPTMTTYLSIPGRLLVTMPDMAHIGISRKIEDEEDRD
                     KIKEILAQLEVPENLGVIVRTAGIGRTKRDLQRDLAYLTRLWKAVNDRIAATKAPAEI
                     YKESDLVIRTLRDVYDADIKRIICDSRDVAQRVCEFLDFAGPRTKCKIQYYTGDQGLF
                     REFGVEEEIRKINSRRVELSNGGSIVIDQTEALVAIDVNSGSYRQHKSAELNALYLNL
                     AAADEIARQLRLRDMGGVIVIDFVDMR"
     gene            complement(1347..1943)
                     /locus_tag="PLK08_08450"
     CDS             complement(1347..1943)
                     /locus_tag="PLK08_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPS53569.1"
                     /translation="MMRAIERLAVRSRLPLHYSQGFNPRPVISLPAARPVGIAGESEI
                     LCINFDAELDSAELLDSLNRNALPGMIFESAMPLATAKATPQITAIVYSMKLAASEVT
                     PLSHKVDELKNSEQWLIHRSVKPSSRRGGRNSREESFVDINLKPRVDVMEICGENFTF
                     TLVPCGDSWAKPCEVLALAGIGGPKNVARLHRVEVRTD"
     gene            1942..2079
                     /locus_tag="PLK08_08455"
     CDS             1942..2079
                     /locus_tag="PLK08_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53570.1"
                     /translation="MSWCEQNRRSPVTANLIAHLKLNIAMPFMRSFSRWSLSVKISRH
                     G"
     gene            complement(2413..>3268)
                     /locus_tag="PLK08_08460"
     CDS             complement(2413..>3268)
                     /locus_tag="PLK08_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPS53571.1"
                     /translation="EFLFLSSDTEPTEEDHYIAYRKVIEQCAGKPVIIRTLDLGADKY
                     TQSRARNPERNPFLGCRSIRLCLQNLDMFKHQLRAILRASVGANVQILFPLVTTITEL
                     RQAKALVRDVSEDLEDEGVEHNADIPIGIMVEVPAAALQAAAFAKEVDFFSIGTNDLV
                     QYTLAVDRGNEKVANMFTAAHPAVLRLIKEVIRTGQQNDIPVSLCGEIAGDPEYTLLL
                     LGYGLRKFSCTPLAIPEIKKVIRSVTLTQAMEVARIVARLDTDQEISSYLRAVTKKVL
                     PQVYGDGE"
BASE COUNT          784 a          852 c          749 g          883 t
ORIGIN      
        1 tcccgcatat cgacgaagtc tatgactatc acacccccca tatcccgcag cctcaactgt
       61 cttgcaattt catctgccgc cgccagattc agatacaacg cattcagctc cgcactttta
      121 tgctgacgat agctgccgga attgacgtcg atagctacca atgcctcggt ctggtcgatg
      181 actatcgacc cgccgtttga aagttccact ctgcgggagt tgattttgcg gatttcctct
      241 tccactccga attcgcggaa aaggccctgg tcgccggtat aatactgaat tttacatttc
      301 gtacgtgggc cggcaaaatc aagaaattcg caaaccctct gcgcaacatc tcggctgtca
      361 cagataatac gttttatgtc agcgtcatag acatctcgca acgtacggat gacaagatcg
      421 gattccttat aaatttccgc cggcgctttc gtagccgcga tgcgatcatt gactgctttc
      481 cacagtcgtg tcagatacgc caaatctctc tggaggtctc gtttggttct gccgatgcct
      541 gccgtacgaa cgattacgcc cagattttcc ggcacttcaa gttgcgcgag gatttccttg
      601 attttatcgc gatcttcttc gtcttcgatt ttgcggctta tgccgatgtg agccatatcg
      661 ggcatggtca cgagcagccg gcctggtatc gaaagatacg tcgtcatggt cgggcctttt
      721 gtgccgatgc cctccttggt catctgtacc acaacttcct ggccgggccg gagacattcc
      781 tgcatgggcg gccgcgactt gtgcgagctg cgacggccga cgctctcaag ctgtctggac
      841 ggcgtaggaa aatacttggg gtgaaggtcg gagatatgca aaaaaccgtt tttcgggccg
      901 ccgtaatcta taaatgcagc ctgtatcgac ggttcaatgt tcgttacgcg gcctttataa
      961 atattcccga cctggctggc agcacttgca cgttctgaat ataattcctg cagtttgttt
     1021 tcggccaaaa ccgctattct gcattcatgg ccttcaatgg tgtttatcaa catgagtttc
     1081 gacatatttt gaatatcctc tataaataaa ttattcaaac tgaccgattt aacggtttcc
     1141 agcatatcag ccttaaaggt aaatgtttat tttatatatg gttatgattc aacagcaatc
     1201 gtttatcaat ctatttttgc caaattgaca atttatgtaa ctttttaaaa taagtattta
     1261 ccgaaccaac tatcgtcaac cggtcacttc gagtagatta caaatttttt cccaaatcaa
     1321 ttttgagagt tattttaaaa aatatttcag tcagttctga cttcaacgcg atgtaatctg
     1381 gcgacattct tcgggcctcc gatgccagcc agagccaata cctcgcacgg ttttgcccaa
     1441 gaatctccgc acggcacaag cgtaaacgta aagttctcgc cacatatctc cataacatcc
     1501 acacgaggtt tcaggttgat atcgacaaaa ctctcttcac ggctattgcg tccgccgcgg
     1561 cgagaagacg gctttacgct gcgatgaatc agccattgtt ccgaattttt caattcatcc
     1621 accttgtgcg aaagcggagt gacttcactc gcagcaagtt tcatggaata gactatggct
     1681 gtaatctgcg gggtggcctt agccgtcgcg agcggcattg cggattcaaa aatcattccc
     1741 ggcagagcat ttcgattaag tgaatccagc aattcggcgg aatcgagttc cgcgtcgaaa
     1801 ttgatacaga gaatttccga ttcgccggct attccgacgg gccttgcagc aggcagggag
     1861 ataaccggcc gcggattgaa tccctgcgaa taatgcagcg gcaatctgct gcgcaccgcc
     1921 agccgttcta tggctctcat catgtcatgg tgcgaacaga atcgcaggtc acccgttacg
     1981 gccaacctga tagcccatct gaaactcaat atcgccatgc ctttcatgcg aagtttttcg
     2041 cgatggtcgc tgtcggtaaa aatttcccga cacggctgac ctcagcggca gattcgcaat
     2101 aaatatctgc gcttccggca gcctgagaaa gtccggatta ttttttatag cagcttctga
     2161 aaataaataa ccgtgaatgt tcgataacat ctgcaaacat gaatccgtcg ttttacgctg
     2221 tcgttactgc atattatgga attaaccata ccaggcgaaa cggcagatta ttcacgttat
     2281 ttcaaattac ttcaaaaact actccggacg ttcttgcata accatatcgc aattgtaaga
     2341 ccggttcccc aaaaattccg gtttcaccgc ttatgccggt agcagtgaaa cgggaacggg
     2401 aacttggtac tgtcattctc cgtcgccata gacctgaggc agaactttct tggtcacagc
     2461 ccgcaggtaa ctggatattt cctggtcggt atccagtctt gcgacgattc tggcaacttc
     2521 cattgcctgc gtaagtgtta cggaccggat aacttttttt atttccggta tagccagcgg
     2581 cgtacatgag aacttccgta agccataacc caacagaaga agcgtatatt ccgggtcgcc
     2641 cgcaatttca ccgcaaaggc tgaccggtat gtcattctgt tggccggttc ggatgacttc
     2701 cttaataagt ctcagcaccg ccggatgtgc cgcagtaaac atgttagcga ccttttcgtt
     2761 gcctctgtcg accgcgagcg tatactgaac gagatcgttc gttcctatcg aaaagaagtc
     2821 tacttctttc gcgaacgccg cagcctgaag cgcggcggct ggcacttcaa ccataattcc
     2881 tatcggaatg tccgcgttgt gttccacgcc ttcgtcttca agatcttcgc tgacgtcacg
     2941 caccagagct ttggcctgcc gaagctcggt aatcgtcgtg accaacggaa ataatatctg
     3001 aacattagcg ccgacgctgg ctcgcagtat cgcgcgaagc tgatgtttga acatatccag
     3061 attctgcagg caaagccgta tcgaccggca gcccagaaac ggattgcgct caggattacg
     3121 ggcgcggctt tgagtatatt tgtctgcgcc gaggtcgagc gtgcggatta tcaccggctt
     3181 gccggcgcac tgttctataa ccttgcgata ggcgatataa tggtcttctt ccgtcggctc
     3241 ggtgtccgaa ctgagaaaca ggaattca
//
LOCUS       DAONFI010000275         3247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_36875_length_3247_cov_3.112782, whole genome shotgun sequence.
ACCESSION   DAONFI010000275 DAONFI010000000
VERSION     DAONFI010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36875_length_3247_cov_3.112782"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLK08_08465"
     CDS             <1..466
                     /locus_tag="PLK08_08465"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS53572.1"
                     /translation="AELADRWQLLIPAGRTQSEKLRNALDAANVSYVCPEYINRMELA
                     YAAADLVLCRGGAVTVAELTAVAKPAVILPYPYHRDRQQYLNAQPLCTAGAAICIDDR
                     IDPAANRELFKKVLFPLMASEEKLNEMHSHALKRTNRGAAAMIARWLTSSDN"
     assembly_gap    756..765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <766..1539
                     /gene="queG"
                     /locus_tag="PLK08_08470"
     CDS             <766..1539
                     /gene="queG"
                     /locus_tag="PLK08_08470"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPS53573.1"
                     /translation="DVETRFYPKKKFPLAKSVIVLAMSFAPSQISTPNPCEGYICLYA
                     RGRDYHKVLKKRAEKLCTQMRDNFGTFDYKICVDTAPVAERSLAVRAGLGFVGKNCLL
                     IIPGSGSMVVLCEIFCSLEIQSTQSDEKFSCGECRKCLDACPTNALNGLGELDASRCI
                     NALTIEYDGKIPQKIAEKMGGRIFGCDDCMLACPHNTAIAPGDEELRNNDFVAPQIKD
                     VLNWTHDDWDSFTRGHSTRRARLEQFIRNAKIADDNIKS"
     gene            complement(1552..2730)
                     /gene="trpB"
                     /locus_tag="PLK08_08475"
     CDS             complement(1552..2730)
                     /gene="trpB"
                     /locus_tag="PLK08_08475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPS53574.1"
                     /translation="MSGCFGQYGGQHVPPQLKAVLDELAKAFNDAWNDESFKAEYYRS
                     LKEIANRPSMLYLAKNLTAKLGGAKIYLKREDLNHLGAHKINNTLGQGLLARRMGKKR
                     LIAETGAGMHGAATAATAAVLGMECEVHMGAVDVKRQEINVMRMELMGTKVVPVTFGQ
                     QTLAEAVDSAIAAWMEHIDDTFYVLGSVCGPHPYPLIVREFQKIIGEETRRQMLETEG
                     RLPDYAIACVGGGSNAAGMFYSMLEDSTKLIGVEAMGRGPQPGQHAASMTLGKLGILH
                     GSKCYLIQNEDGTPGNVYSISAGLDYPGVGPEHCQWKDSGRVQYESATDDEAIDALVE
                     LCRTEGILPAVESAHAVAYAIKLAPTLGKDKIIVINLSGRGDKDCMQIRDALKQRGKI
                     "
     gene            complement(2883..>3247)
                     /locus_tag="PLK08_08480"
     CDS             complement(2883..>3247)
                     /locus_tag="PLK08_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53575.1"
                     /translation="FVDHSDAVEQTAVFTSMKNIASSMILFKVNNGDYPADRNPAQCP
                     AGFADYTQLDWVNEQPLGGQWDWDKGVFGFTAGISVYMPDKETSYMQEMDRRFDDGNL
                     NTGSFRARSQGYIYIIEP"
BASE COUNT          743 a          831 c          870 g          793 t
ORIGIN      
        1 ggcggaactt gctgaccggt ggcagctgct gataccggcc ggcaggacac aaagcgaaaa
       61 acttcgcaac gcactcgatg cagcaaacgt aagttacgtc tgtccggaat atataaacag
      121 gatggagctt gcatatgctg ccgccgattt ggtactgtgc aggggcggag cggtcacggt
      181 ggctgaactg acagccgtcg caaaaccggc tgtaatcctg ccatatccat atcaccgcga
      241 caggcaacag tacctcaacg cccagccgct atgtacggcc ggcgctgcaa tatgcatcga
      301 tgaccggata gacccagccg ccaacagaga actatttaaa aaagtgcttt tcccgctaat
      361 ggcatccgag gaaaaactga atgaaatgca ttcacacgca ctcaagcgga caaaccgcgg
      421 agcggcagct atgattgccc gatggctgac gtcgtctgat aattaacgac tctcaaaatt
      481 tttattttga cgtcatgccg atgcaacgaa gctgcctaag cataggaaat gtgataattc
      541 ggattcttcg cctcgttttc tacatgacaa tctgaatttg ataattgcaa taatatggtg
      601 tctcactcat ctacagaacg gcttgaattc gtccggcgga tggcggcggc attggggttc
      661 tgcgcgtgcg gcgtgacaac ggctcagccg ctggacgatg aatgcattac acgatatcgt
      721 cattggctgc agtgcggcaa agctggggaa atggannnnn nnnnngatgt cgaaacacgt
      781 ttttacccca aaaaaaaatt tccgctggca aaaagcgtaa tcgtcctggc gatgagcttt
      841 gcgccgagtc agatttcaac accgaatccc tgtgagggtt acatctgtct ttacgcccgc
      901 ggccgggatt atcacaaggt gctcaaaaaa cgagccgaaa agctgtgcac gcagatgcgc
      961 gataacttcg ggacgtttga ttataaaata tgcgtggata cagctccggt ggcggaaagg
     1021 tcactggctg tgcgggcagg gttgggtttt gtggggaaaa attgcctgct gataattcca
     1081 ggctccggca gtatggtcgt attgtgcgaa attttctgct cacttgaaat ccagtctacg
     1141 cagtctgatg aaaaattttc atgcggcgaa tgcagaaaat gtctcgacgc ctgtccgacg
     1201 aatgccctga acggtttggg cgaacttgac gcttcacgtt gcatcaacgc acttacaata
     1261 gagtacgacg gaaagattcc gcaaaaaatc gcagaaaaaa tgggcggcag aatcttcggc
     1321 tgcgacgact gcatgttagc ctgcccgcat aataccgcga ttgcgcctgg cgacgaagaa
     1381 ttgcgcaaca acgatttcgt cgctccgcaa atcaaagacg ttctgaactg gacgcacgat
     1441 gattgggact cttttacgcg cggccattca acgcgacgtg ccagacttga acagttcatc
     1501 cgaaatgcaa aaattgcgga tgacaatata aaatcataag ccgccgtcga ttcagatttt
     1561 ccctcgctgt ttgagagcgt cgcgaatctg catgcaatcc ttatcgcctc tgccggacag
     1621 attgatgacg attattttat ctttacccag tgtcggtgcg agttttatcg catacgccac
     1681 ggcgtgtgcg ctctcgacgg ctgggaggat gccttccgtg cgacagagtt cgacgagtgc
     1741 gtcgatggct tcatcgtcgg tggcggattc gtactgtacg cggccggagt ctttccactg
     1801 gcagtgttca gggcctacgc ctggataatc caggccagcc gatatcgagt acacattgcc
     1861 cggcgtgccg tcttcgttct gaatgaggta gcacttgctg ccgtgcagta tgcccagctt
     1921 gccgagcgtc atggatgcgg cgtgttgtcc tggttgcggg cctcggccca tggcttcgac
     1981 gccgatgagt ttcgtactgt cttccagcat cgaatagaac atgcccgctg cgttactgcc
     2041 gccgccgacg caggcgatgg cgtagtcagg cagacggccc tcggtttcaa gcatctgacg
     2101 gcgggtttcc tcgccgatga ttttctgaaa ttcacgcact atcagcggat acggatgcgg
     2161 cccgcatacc gagccgagca cgtaaaacgt atcgtcaata tgttccatcc atgcggcgat
     2221 ggcggaatcg acggcttcag ccagcgtctg ttggccgaag gtgacaggta cgactttcgt
     2281 gcccatgagt tccatacgca taacgttgat ttcctggcgt ttaacgtcca ccgcgcccat
     2341 gtgcacttcg cattccatgc ccagtacggc agctgtcgct gcggtggctg cgccgtgcat
     2401 gccggcgccg gtttcggcta tcagccgttt tttgcccatg cgccttgcga gaagaccctg
     2461 gcccagagtg ttgtttatct tgtgagcgcc gagatgattg aggtcttcac gcttgagata
     2521 tatcttcgcg ccgccgagtt tggcggtgag atttttggct aagtaaagca tgctgggacg
     2581 gttggcgatt tctttcagag agcggtagta ttccgccttg aacgattcgt cgttccatgc
     2641 gtcgttgaac gcttttgcca gctcatcgag aacagccttg agttgcggcg gtacgtgctg
     2701 gccgccgtat tggccaaaac agccggatat attgggtttg gtaaacatat tttaaacaac
     2761 ctttcctttt tccataaaat ttacgaaaac atgattatat tgcgacactg ccgtcaggca
     2821 agtcacaaaa caactttatt tgagtgaaaa tatcggcagc tgtcggcaat ggcagctgat
     2881 gttcagggct cgatgatgta gatatatccc tggcttcttg cccgaaaact tccggtattg
     2941 agattgccgt cgtcgaatcg ccggtccatc tcctgcatgt aggaggtttc tttatccggc
     3001 atatatacgc tgatgcctgc ggtgaatccg aacacgccct tgtcccagtc ccattggccg
     3061 ccgagcggct gttcgttcac ccaatccagt tgagtataat ctgcaaagcc ggctggacat
     3121 tgtgccggat tcctgtcggc aggataatcg ccgttgttga ctttgaacag aatcatcgac
     3181 gacgcgatgt ttttcatgct cgtgaagacg gccgtctgct cgacagcatc gctgtggtct
     3241 acgaatt
//
LOCUS       DAONFI010000276         3217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_37360_length_3217_cov_4.224541, whole genome shotgun sequence.
ACCESSION   DAONFI010000276 DAONFI010000000
VERSION     DAONFI010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37360_length_3217_cov_4.224541"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PLK08_08485"
     CDS             complement(<1..1401)
                     /locus_tag="PLK08_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPS53576.1"
                     /translation="MYLEFYRLKEFPFALTCDERFFYESAVHSETLANMIYTVQSHKG
                     MVLITGDVGTGKTFLVNMLISRLAGNCIAVNMQNPPQSGKQLVRGLAVQLGMRIDPTQ
                     DKISLLENIERHLARAHQRGRLVALLLDEAQDLPDDTLREIRLLANWERNGQRLLQII
                     LVGQPELRNRMLEPQWESFRQRIVLTYNLGHMSRDDVSAYIDYRLKIASGEGCLAKFS
                     AQAKEDIYFATQGIPRLINVLCDNALLTGYTRKVTEISHELMTDVLKEASLWDFGAPP
                     TEAKPPQEEHPLTPQPAAATTDDTAASEPVFVHMDKTTPPPSSDEVINLKDIEEELDR
                     ASKDIAQSDLPSLGDKPSLQESSPEDDETVDFQPGYHIVTPPEPVKTPQRTPIYELSQ
                     DHITPQGVLSAMSAARPTAKQRSIDQIDNKLHMSDLLIAHKDRGGPITEEFRALRTSL
                     LATATDERFCYLITSPE"
     gene            complement(1413..3083)
                     /locus_tag="PLK08_08490"
     CDS             complement(1413..3083)
                     /locus_tag="PLK08_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53577.1"
                     /translation="MPEQTTSISFNEILRVFMKYRRVCLLCAALFCIIALQVAQRVMP
                     KKYTSSAVFERRSDVATQQTNDKSSESFDTIKKTLPQDFITQSAMEQLVDELEADGLI
                     KPLEREYDTGQLTNKGRRERQQLIDNMKKNISIEFEVRSQNVDYVIVAYKATDPLLAA
                     KVPNELIKIYINNISQSIRTRLQDSLDYLISRKQQAEKDYSIINEKRGDFEQKNVGIT
                     MGTPIVILQHIQETKAKKAALESQLMLAQKEYAKLMKAREESDSNNSPSQVIYGPNPE
                     LAQMDKDLQNYRNQLETARVLDHMTDEHPVVKSLQASIKMMEDRIKRTPAEIVVQKIY
                     SQGVGLQSFAAMAAGKQEEIEQIKANLTLATTQLKGFEAAQESYVEGKKIYGQLMEDW
                     QSKRNDYDYWDRQVKNVKDILAAEVSKRRTLLSEINRAEVPLGPSSPNILMIFAFAFV
                     GGIAFGMAVIFLLYKLDRTVSTPEEAARIFNLPVYGVISEIIPPSVKARRKFSTWLIE
                     PAVALVLLAAIAVSTYSTVLWLQEPDEYARWNSSPIEFIQHKIEKTMN"
BASE COUNT          626 a          815 c          909 g          867 t
ORIGIN      
        1 ttcaggtgaa gttatgagat agcagaaccg ctcgtccgtt gccgtcgcaa gcagggaggt
       61 acgcaacgcg cgaaactcct cggtaatcgg gccgccgcga tccttatgcg caatgagcag
      121 gtcggacatg tgaagcttgt tgtctatctg gtctattgaa cgttgtttgg cggtcggcct
      181 ggctgccgac atggccgaca ggacgccctg cggcgttatg tgatcctggg acaactcata
      241 tatgggcgta cgctgcggtg tttttaccgg ttcgggcgga gtgacgatgt gataaccggg
      301 ctgaaaatcc acggtttcat cgtcttccgg cgacgattcc tgcagcgaag gcttatcgcc
      361 gagtgaaggc agatcgctct gggcgatatc cttcgatgcg cggtcgagtt cttcttcaat
      421 atccttgaga tttatgactt catcgctcga cggcggcgga gtagttttgt ccatgtgaac
      481 gaagaccggc tcggaggcgg cggtatcgtc cgtggtagcg gcggcaggtt gcggcgttaa
      541 ggggtgttct tcctgcggcg gcttagcctc agtcggcgga gcgccgaaat cccacaaaga
      601 ggcttccttg agaacgtccg tcattaactc gtgacttatc tctgtgactt tacgggtata
      661 accggtaagc agtgcgttat cgcaaagcac gtttataagg cgaggaatgc cctgggtggc
      721 gaaatatatg tcctctttag cctgggctga aaattttgcc aggcagcctt cgccggacgc
      781 tatttttaaa cgataatcta tatatgccga aacatcatcc ctggacatgt ggccaaggtt
      841 atacgtcaat actattctct ggcggaacga ttcccactgc ggttcgagca tacggttgcg
      901 aagctccggc tggccgacca ggattatctg caacagccgc tggccgttac gctcccagtt
      961 cgccaataat cgtatttctc gcaacgtgtc atccggcaga tcctgtgctt catccagcag
     1021 cagcgccacg agacggcctc gctgatgcgc cctggccaga tgtctttcta tgttttcgag
     1081 aagcgatatc ttgtcctggg tcgggtctat cctcatgccc agctgcacgg caaggccgcg
     1141 cacgagctgt ttgccggatt gcggcggatt ctgcatgttg acggctatgc agttgccggc
     1201 cagacggctt atcagcatgt tgacaagaaa agttttgccc gtgccgacgt cgccggttat
     1261 caggaccatg cctttgtggc tctggacggt gtatatcatg ttggccagag tctcggagtg
     1321 aaccgcgctt tcataaaaga accgctcgtc gcacgtcagt gcgaacggga attctttcag
     1381 cctgtaaaat tccagataca tctgcgaaag cctcagttca ttgtcttttc aattttgtgt
     1441 tgtatgaatt ctatcggcga cgaattccat cgtgcgtatt catccggctc ctgcagccac
     1501 aaaacggtgc tgtacgtgga gacggcgatg gcggcaagca gtacaagggc gactgccggc
     1561 tcaatcagcc atgtagagaa ttttctgcga gccttgaccg acggcggtat tatttcgctt
     1621 atcacgccgt agaccggcag attgaatatg cgagcagcct cttcaggcgt tgaaacggta
     1681 cggtcaagtt tgtataacag gaaaattacg gccatgccga atgcaatgcc gccgacgaat
     1741 gcgaaagcga aaatcatgag tatgttcggc gacgacgggc ccagcggcac ttccgcgcgg
     1801 tttatttcgc tcagcagcgt gcgacgcttg gaaacctcgg cagccaggat gtccttgacg
     1861 tttttgacct gccggtccca gtaatcgtaa tcgttgcgtt tggactgcca gtcttccatt
     1921 agctggccgt aaatcttttt gccctcgacg tagctttcct gggcggcttc gaagcccttg
     1981 agctgcgttg tggcgagagt caggttcgcc tttatctgtt ctatttcttc ctgtttgccg
     2041 gctgccatcg ccgcgaatga ctgcaaaccc acaccctgcg aataaatttt ctgtaccacg
     2101 atttccgccg gcgtacgctt tatgcggtct tccatcatct ttatgctggc ctggagggat
     2161 ttgacgacgg gatgctcgtc ggtcatgtga tccagaaccc tggcggtctc aagctggttg
     2221 cgataattct gcaaatcctt gtccatctgc gccagttcgg gattggggcc gtaaatgacc
     2281 tgcgagggcg agttgttcga atcgctctct tcacgagcct tcatgagttt ggcgtattct
     2341 ttctgggcga gcatgagctg gctttccagg gcggcttttt tggctttggt ctcctggatg
     2401 tgttgaagta tgacaatcgg cgtgcccatg gtaatgccga cgtttttctg ctcaaagtcg
     2461 ccgcgttttt cgttgatgat ggagtaatct ttttcagcct gctgtttgcg gcttatgaga
     2521 tagtcgaggc tgtcctgcaa tcgggtgcgg atgctctggc tgatgttgtt tatatagatt
     2581 ttgataagtt cgttgggaac tttggcggct agaagggggt cggtggcttt atatgcgacg
     2641 atgacgtagt cgacgttctg cgagcggacc tcgaattcga tggaaatgtt tttcttcatg
     2701 ttgtcgatga gctgctgacg ttcacgacgg cctttgttgg taagttggcc ggtgtcgtat
     2761 tcccgttcca gcggctttat aaggccgtcg gcttcgagtt cgtcgacaag ctgttccatc
     2821 gcgctttgcg taataaaatc ctgcgggaga gtctttttga ttgtatcgaa cgattcgctg
     2881 cttttatcgt tggtttgctg cgtggccacg tcactgcggc gttcgaatac ggcactggag
     2941 gtgtattttt tcggcattac ccgctgggcg acctgcaggg ctatgatgca gaaaagcgct
     3001 gcgcagagca gacataccct gcggtatttc atgaataccc gcagaatttc gttaaacgaa
     3061 atggatgtcg tctgttccgg catatatcac cgcgtgttta ttggcctccg ccgcctccgc
     3121 cgccgaccca tacgccgttg tcgtatgacg ggtcaaagat ggctcgaatg tcccatatgc
     3181 cctgcctggt tgcatcgagc ggcgaaacag cgttcca
//
LOCUS       DAONFI010000277         3214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_37410_length_3214_cov_4.021842, whole genome shotgun sequence.
ACCESSION   DAONFI010000277 DAONFI010000000
VERSION     DAONFI010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37410_length_3214_cov_4.021842"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PLK08_08495"
     CDS             complement(<1..1031)
                     /locus_tag="PLK08_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53578.1"
                     /translation="MIDTDKQQATRVVTVIKTALIALPIAAMAVLTLTSSLFDNPSDP
                     SGTYKTPITAPQPQNRLFDTNAIPESLRKYRQINNLPTGLTRPMDIAWYDGRLYIAGD
                     DCIIVMDPDNGQILQKFRIPRHPTAISVNDSFVAAISQDSLYSMPTGGSEFLQVAQFP
                     ADTSNLVAVAANFENFFVADSFSGTIYKIGLNGNILNTIHPQTGDMKFNVTSTGDFDV
                     IFGAEGMLWIADPGRGQVMAFNTSGENSRNLNWGKHGERLANFSGAYNPVHIVMLPNT
                     NIVTAELDPPRIKVYSDAGTLLAVVAWENTLPKHIRRLNLSTDAAGHIYVLDDVSNTI
                     RIYEKLTPA"
     gene            1252..1656
                     /gene="speD"
                     /locus_tag="PLK08_08500"
     CDS             1252..1656
                     /gene="speD"
                     /locus_tag="PLK08_08500"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPS53579.1"
                     /translation="MEQTITQAPPAVEYCGNNGYIKFAGIHLIIEIWQGRHLDDAQKI
                     EQILREAIDACEANLLYMYLHNFGEGAGITGVGVLSESHISIHSWPEFEYAALDLFVC
                     GTKDPYKALPVFQKGFGGKMQVTEHKRGVMPQ"
     gene            1653..2507
                     /gene="speE"
                     /locus_tag="PLK08_08505"
     CDS             1653..2507
                     /gene="speE"
                     /locus_tag="PLK08_08505"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPS53580.1"
                     /translation="MKASAAYAREKWPDILEVSYELKDSFHSEQTPFQSMELVETRRF
                     GRMLLLDGMVQTTEKDEFIYHEMMTHVPIISHGHVRKVLIIGGGDGGILRETLRHSDV
                     EKVTIVEIDSRVVDFSQKFLPSISAGAFDDSRTELVIGDGAGFVASTDEKYDVVIVDS
                     PDPIGPASVLFSREFYKNVKNCMTQYGIMTRQCGSTWMQPDELPAAYAIVKPIFTYNT
                     AYVFAVPTYIGGFFSSLMCTNGSEHVLDLATLEKRFEAIKDQTRYYNPGVHFGAFALP
                     GYVKEKLV"
     gene            2504..2923
                     /locus_tag="PLK08_08510"
     CDS             2504..2923
                     /locus_tag="PLK08_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HPS53581.1"
                     /translation="MSCCSNNGTEVITHGQVPVGRNVVFGWELVLDLYDCDPAIMSSE
                     KELRRFTLELCKVINMTPYGEPWTPYFGENCEHTKGYSLLQFIETSSITGHFSEGTKT
                     AYINIFTCKAFDEKKAEEFTVKFFKARTHVSRFMTRA"
     gene            2978..>3214
                     /locus_tag="PLK08_08515"
     CDS             2978..>3214
                     /locus_tag="PLK08_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPS53582.1"
                     /translation="MSWKVAIMGATGAVGTEFLKILEERNFPLKSLKLLASERSAGKK
                     LPFRGEMLPVELLTEESFKGIDIVFASAGAGTSKE"
BASE COUNT          807 a          710 c          864 g          833 t
ORIGIN      
        1 atggctggag tcagtttttc ataaattcgt atcgtgtttg atacgtcgtc gagaacgtag
       61 atatggccgg cggcgtcggt ggagagattc aggcgacgga tgtgtttcgg cagggtgttt
      121 tcccatgcaa ctaccgcaag cagcgtgcct gcgtcggaat aaacctttat acgcggcggg
      181 tcgagttcgg cggtgacgat atttgtgttc ggcagcatga cgatgtgtac cggattgtat
      241 gcgccggaaa aattcgccag cctttcgccg tgtttgcccc agttgagatt tcgggaattt
      301 tcgccgctgg tattgaacgc cataacctga ccgcggccgg ggtcggctat ccacaacatg
      361 ccctctgcac cgaatatgac gtcaaaatcc ccggtactgg tgacattgaa tttcatgtcg
      421 ccggtttgag gatggatggt attcagaata ttgccgttga ggcctatctt ataaattgtg
      481 ccgctgaaag aatcggcgac gaaaaaattc tcgaaattgg cggctacggc tacaagatta
      541 ctcgtgtcgg ctgggaattg tgcgacttgc aggaactcgc tgccgccggt tggcatggaa
      601 tacaggctgt cctgagagat ggctgcgaca aatgaatcat tcacgctgat ggcagtgggg
      661 tgacgaggga ttctgaattt ctgtaaaatc tggccattat ccgggtccat gactatgatg
      721 cagtcatcgc cggcgatata aagtcggcca tcataccacg ctatgtccat cggcctggtt
      781 aaaccagtcg gcaagttatt tatttgccgg tacttccgca gtgattccgg tatcgcattg
      841 gtatcgaaca gccgattttg cggctgcggc gcagttatcg gggtcttata agtccctgat
      901 gggtcagaag gattgtcgaa cagggaagat gtcagagtca gaacagccat tgccgcgatg
      961 ggcagagcaa tcagagcggt ttttatgacc gtgacgactc tggttgcctg ttgtttgtca
     1021 gtgtcaatca aggctgcagc ctccttgcaa tatcactgcc ggcaatatta tcaaaaaaaa
     1081 gcataaatcg cgagtttttt tgatgaaaag atgaatttta cagctaaaat atttttgcaa
     1141 aagtgtagga attgaagatt gacagagagc gaaattgcgt tatattcatc tctcttttaa
     1201 agaaaaggta aaggtatata tatgggtttc agctagcgag aggatacttt attggaacag
     1261 accatcactc aagccccgcc ggcggtcgag tactgcggca ataacggtta tatcaagttt
     1321 gcaggcattc atcttattat cgaaatctgg cagggcaggc atctcgatga tgcccaaaag
     1381 atagagcaga ttctgcgcga ggcgatagac gcctgtgagg cgaatctgct ttatatgtat
     1441 ctgcacaatt tcggcgaagg tgcgggtatc acgggcgtag gcgtgctgag tgagtctcat
     1501 ataagtatcc attcgtggcc ggaattcgaa tatgcggcgc tggatctttt cgtttgcggc
     1561 acaaaagatc cgtacaaggc gttgccggtt ttccaaaaag gttttggcgg caaaatgcag
     1621 gttaccgagc ataaacgagg tgtaatgccc caatgaaggc ctctgccgca tatgcccgtg
     1681 agaaatggcc tgatattctg gaagtcagtt acgaactgaa agactccttt cattccgaac
     1741 agacgccgtt tcagtccatg gaacttgttg aaaccaggcg attcggccgc atgctcctgc
     1801 tggacggcat ggtgcagacg actgaaaagg acgagttcat ctatcatgag atgatgacgc
     1861 atgttccgat aatatcgcac ggccatgttc gtaaggtgct cataatcggc ggcggcgacg
     1921 gcggcattct ccgcgagacg cttcgtcatt ccgacgtcga gaaagttacg attgtcgaga
     1981 ttgattccag ggttgtggat ttttcccaaa aattccttcc ctcgataagt gccggcgcat
     2041 tcgacgacag tcggacggaa cttgttatag gcgatggcgc cggctttgtg gcgagtacgg
     2101 atgaaaaata cgacgttgtc atagttgatt cgccggatcc gatagggccg gcgagcgtgc
     2161 ttttcagccg tgaattttac aaaaacgtta aaaattgcat gacgcaatat ggcataatga
     2221 ccagacaatg cggttcgaca tggatgcagc cggatgaact tccagccgct tacgcaattg
     2281 ttaaacctat ctttacgtat aataccgcat acgtcttcgc cgttcccacg tacatcggcg
     2341 gctttttcag cagtttgatg tgtacgaacg gttctgaaca tgttttggat ctggccacgc
     2401 tcgaaaaacg gttcgaagcg attaaggacc agaccaggta ttataatccc ggcgttcatt
     2461 ttggggcgtt tgccctgccc ggctatgtca aggagaaatt ggtatgagtt gttgtagcaa
     2521 taacggtact gaagtcatca cgcacggcca agtgcctgtg ggtcgcaatg tcgtcttcgg
     2581 ctgggaactt gtgctggacc tctatgattg cgatccggca atcatgtcgt ccgaaaaaga
     2641 gcttcgcagg ttcacgctcg aattgtgcaa ggtcatcaac atgacgcctt acggcgagcc
     2701 gtggacgcct tatttcggcg aaaactgcga acatacaaaa ggctatagcc tgcttcagtt
     2761 tattgaaacc agttccatta ccggtcactt ttccgaagga actaaaaccg cctatatcaa
     2821 catatttacc tgcaaggcgt ttgacgagaa aaaggcggaa gagtttactg taaaattctt
     2881 caaagcccgg acgcacgtct caagattcat gactcgtgca tgattcggtt gtgtctgaat
     2941 acagacagtt tatgagaata ggataaaggt cgttaagatg agttggaaag ttgccatcat
     3001 gggtgccacc ggtgcggtcg gcacggaatt tttaaaaata ctcgaagaac gtaattttcc
     3061 gttaaaaagc ctcaagttat tggcttcaga gcgttcagcc ggcaaaaagc tgccttttcg
     3121 tggcgaaatg ctgccggtgg agcttttgac ggaagaatcg ttcaagggta tcgatatcgt
     3181 atttgcttcg gccggtgccg ggacctccaa agaa
//
LOCUS       DAONFI010000278         3208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_37502_length_3208_cov_4.770377, whole genome shotgun sequence.
ACCESSION   DAONFI010000278 DAONFI010000000
VERSION     DAONFI010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37502_length_3208_cov_4.770377"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..954)
                     /locus_tag="PLK08_08520"
     CDS             complement(202..954)
                     /locus_tag="PLK08_08520"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53583.1"
                     /translation="MLFSKNVRFLSQKFYSTKRVKPSAIGFTLIELLVVIAIISLLVS
                     ILMPSLNVAKELARRAVCSANLRTVSTGMMMYADDGAGKYPNYSSIHWPVGYFYYDLN
                     PADSTAIARTKTYAGFKQLVPDYIDSTGIFYCPSQKQTDFLAATYPRRNWLNDGYLVY
                     TGYCYWANYIYDSIGLTDEVVTTGVLSNPDTVMASDIMCRDSTPQWNNHEGSNFEGGN
                     VLCNDSHVEWRGDNAVELRINSFSPQPGDFWF"
     gene            complement(1177..1941)
                     /locus_tag="PLK08_08525"
     CDS             complement(1177..1941)
                     /locus_tag="PLK08_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53584.1"
                     /translation="MKHAVAAIIALAGIMSLACGDTVGYWKFDEGSGTTIVDTTGLNA
                     NGTLDEWNGSAWVTQTNPTWVQGASGWAVGFNGTNQGAIVPDSSALDVTTAVTLEAII
                     KPSTFSGTGDIISKDSAYQLKLGGDLKSINFLFRSGSSWYGGGVILSTALSSDEWTHI
                     AITYDSTLESNNIKVYVDYELVSQKTVTGTINTTTSLLTFGAEQVGVEYYKGDIDAIR
                     ISNVALSSSEFLPIPEPATLSLIAAGSLALLGCRKK"
     gene            complement(2024..3196)
                     /locus_tag="PLK08_08530"
     CDS             complement(2024..3196)
                     /locus_tag="PLK08_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPS53585.1"
                     /translation="MDYVAVKKSLSCASVKPRYFRLAEAIQDCIESGSLKRSDKIPSQ
                     RELMTNLGLSDSTVNRAIRELEARGIVYSRQGQGTFVADKVKSQATTIVALVPSRPNV
                     SYDTEIVWHAEQAAHRGGYELMVCNTEDNFSKVDTYLDRLLSKDANMILGVLYVPVAT
                     NCDDYYERNMERIARIQRLGIKVVLCDRNFLNQPDAIAAYKLDAVYSDDMRGSRAVVE
                     HFISLGRRRIALLSGPMDSNVENRITGYRQALHDNGLSYMSELVKFVDSYERPEDVAP
                     MVDELLATPDGIDAIFAINDRAAEAVLECLQSRGVCVPDDVSLSGYDNMEHSRYLSIP
                     LTTVDRANGEIGEKAVELLLEQVENPRNYPRHIALPTQLIIRQSSCSSEQLAEMKA"
BASE COUNT          963 a          805 c          576 g          864 t
ORIGIN      
        1 ctgccataca aggcaaacat gaaacaaccg cggctagaaa catcacaact cttcggcgtg
       61 aattaaataa ttctgacatc aaaaatctcc ctataataat caaactataa aatacgagac
      121 gaaactgctg aaaacagcac aaaattattt cctacaaata aagattgcta tatttataat
      181 ggagagtatg gaaaaagatt attaaaacca gaaatcgcca ggctgaggtg aaaatgaatt
      241 tatgcgaagc tcaaccgcat tatcaccacg ccattcgaca tgcgaatcat tacacaacac
      301 attgccccct tcaaaatttg aaccttcatg attattccat tgcggagttg aatcccgaca
      361 cataatatca gaagccataa cggtatcagg attacttaaa acgcctgtag ttactacttc
      421 atctgtcagc ccgatactgt catatatata atttgcccaa taacaatacc ctgtatacac
      481 caaataacca tcattcaacc aattcctacg agggtaagtt gcagcaagaa aatcggtttg
      541 tttttggctg gggcaataga atattccagt actgtctatg taatcaggaa ctaattgttt
      601 aaacccggcg taagttttcg tacgtgcgat agcggtacta tccgcaggat ttaagtcata
      661 ataaaaatat ccgacaggcc aatgaattga actatagtta ggatatttgc cggctccatc
      721 gtcggcatac atcatcatgc cggtcgacac agttcgcaga ttcgcagaac agaccgcacg
      781 ccgggcaagt tccttggcaa cgttcagcga cggcatcaat atggaaacta aaagcgaaat
      841 aatcgcaatt acaaccaaaa gctctataag agtaaaccct atggctgatg gttttactct
      901 ttttgtagaa taaaatttct gcgataaaaa cctgacattt ttggaaaaca gcatctcagg
      961 tactcctaaa acatagtgtc agtttttcgc gaattaatac aagccataca cattagaact
     1021 tttgtttctt ttaatactac tgtttacaaa cagacatata cgccatacat acttgccata
     1081 atccgcttaa atggcaaaac atatataatt ttcactatac cattatattc taattacaaa
     1141 cttgcaatca gaaattttca tatgttcgta tatcacttat ttttttctgc agcccaacaa
     1201 ggctaaacta ccggcagcaa tcaatgataa cgtagccggt tcaggtatgg gtaaaaattc
     1261 actcgacgac aatgcgacat tcgaaatacg tatcgcatct atatcgccct tgtaatattc
     1321 gacaccaact tgctctgcac caaaagttaa caaactggta gtcgtattta tggtaccggt
     1381 aacagtcttc tgactaacca actcgtaatc cacataaact ttaatattat ttgactccaa
     1441 tgtactatca tatgttatgg caatatgtgt ccattcatcg ctcgataatg ctgtcgaaag
     1501 aattactccg ccaccgtacc aacttgaacc actgcgaaat aaaaaattga ttgatttgag
     1561 atcgccgccc aatttaagct gataagccga atctttactt ataatgtctc cagtgccgga
     1621 aaatgtagaa ggtttaataa tagcttcaag agtcaccgcg gtagtcacat ccaacgcact
     1681 gctatcagga acaatcgcac cctgatttgt gccgttgaaa ccgacagccc agccggacgc
     1741 accttgaacc catgtcggat tcgtctgcgt tacccacgca ctgccgttcc attcatcaag
     1801 agtgccattc gcattcaagc ccgtcgtgtc aacgatagtc gtcccgctac cttcgtcaaa
     1861 cttccaataa ccgaccgtat cgccacatgc aagtgacata atgccggcta acgcgataat
     1921 tgctgccaca gcgtgtttca taattttctc ctgttaaatt tcattgattt taatactctt
     1981 acgttacctt aacataattc atattcagta atgagttttg ccatcatgct ttcatttccg
     2041 ccagttgttc acttgaacaa cttgactgac gtattatcag ttgcgtcggc aaggcaatat
     2101 gcctgggata attccgcgga ttttcgacct gttccaatag taactcgaca gccttttcac
     2161 cgatttcgcc attagcccta tctaccgtcg taagcggaat ggaaagatat cgcgaatgtt
     2221 ccatattgtc ataaccgctt agcgaaacat cgtcaggcac gcacactcca cgcgactgga
     2281 gacattccaa gacagcctct gccgccctgt cattaatcgc aaatatggca tcaatgccat
     2341 ccggagtagc cagaagttca tcaaccatag gcgcgacatc ttccggccgt tcatatgaat
     2401 ccacgaattt caccaattcg ctcatataac taaggccatt gtcatgtaat gcctgcctgt
     2461 accccgttat gcggttttcg acgttcgaat ccataggccc gctcagcaat gctattcgcc
     2521 ggcggcccag tgaaatgaaa tgctccacaa ccgcccttga gccgcgcata tcgtccgaat
     2581 acaccgcatc caacttatat gccgctatcg catccggctg attgagaaaa ttacggtcac
     2641 aaagaacaac tttgattcca agacgctgaa ttctggcaat tcgttccatg ttgcgttcgt
     2701 aatagtcgtc gcagttcgtt gccaccggaa cgtaaagcac ccccaaaatc atgtttgcat
     2761 ctttactcag aaggcgatca agatatgtat cgactttact gaaattatct tcggtattgc
     2821 agaccatcag ttcatatccg ccgcgatgag ccgcctgttc agcatgccac actatttcag
     2881 tatcatatga gacgtttggc cgcgacggca caagagccac aatcgtcgta gcctggctct
     2941 taaccttgtc cgctacaaaa gtcccctggc cctgccgcga ataaacaatg ccgcgtgcct
     3001 ccaactcgcg aatagcccga ttcacagtag aatcgctcaa accgagattt gtcatcaatt
     3061 cgcgttggct tggaatttta tcgctgcgtt tgaggctgcc ggattcgata caatcctgaa
     3121 tagcctccgc caagcggaaa tatcgcggct taactgacgc acaagaaaga ctctttttta
     3181 ctgcaacata gtccacatga aactccta
//
LOCUS       DAONFI010000279         3191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_37770_length_3191_cov_4.159120, whole genome shotgun sequence.
ACCESSION   DAONFI010000279 DAONFI010000000
VERSION     DAONFI010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37770_length_3191_cov_4.159120"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLK08_08535"
     CDS             complement(<1..183)
                     /locus_tag="PLK08_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HPS53586.1"
                     /translation="MRRNILHMGAGSLTYAIREIVQVARRFEDMGLNITWENIGDPIQ
                     KGEKIVPWIRDIMHEIV"
     gene            316..1236
                     /locus_tag="PLK08_08540"
     CDS             316..1236
                     /locus_tag="PLK08_08540"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HPS53587.1"
                     /translation="MVAAIGFRMYNHSPAMMIAKEKAVIVNADDFGLSEGISEGILRA
                     ATEGILTSTTIQANQPLCEKCLRFLKSAPTHLGVGVHLTINGGRPLSIAGQALADENG
                     KLSLTASQTFLTLFKKPSLLAAAEAEFEAQITWALDHGIKPTHLDSHRHSHAYPPLFK
                     RVVRLAKKYNIPFIRQYGEKFIGEGWPGYDPRNLKAHRILNFFGWYVAHRWPSMRGTI
                     GTWGVLQTGRMSAEWLVRAAQAATQVDGVVEIMTHPGLTYDLTPQITRMRDSRREELD
                     ALTNPAVKEAFHQCGIKLINYRDLLNFVAT"
     gene            1381..2199
                     /locus_tag="PLK08_08545"
     CDS             1381..2199
                     /locus_tag="PLK08_08545"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPS53588.1"
                     /translation="MKNEDTFAPLDVARQLLAIESLLGSLDFLPASRNPLKMPKLPAP
                     DIFVQQANEAKFSSSAVIIDEIPVHLTVEEKIARLAELEVEVGKCTGCELCKTRNKVV
                     FGERNPAARLVFVGEAPGADEDAQGRPFVGRAGELLTKMINAMGLRREDVFICNMLKC
                     RPPQNRTPNPQEIQACWSFLVRQLQIIRPEVIVTLGNPATQGLLNTKMGITRLRGEWQ
                     ALPEIAPGLGGIPVMPTFHPAYVLRNYSVDTRKKVWADLQQVMARLNLKTPKKQ"
     gene            complement(2334..2750)
                     /gene="nikR"
                     /locus_tag="PLK08_08550"
     CDS             complement(2334..2750)
                     /gene="nikR"
                     /locus_tag="PLK08_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPS53589.1"
                     /translation="MSELVRFSVSIERPLFDKFEKLVRKAEYENRSEYIRDLIRESII
                     EQNWQSRKQQLGTITITYNHHTRGLVERLLDIQHHFSGKVLANTHVHLEEHICAEMIM
                     VRGTGKEIQSLANHLRREKGVLHAQLAVGCGEEAFE"
     gene            complement(2823..>3191)
                     /locus_tag="PLK08_08555"
     CDS             complement(2823..>3191)
                     /locus_tag="PLK08_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53590.1"
                     /translation="EQAEVDAIIRQYGYSGTENIMARWRACPDGGDLKDLTHATAHLI
                     HGSTDGRFTVTYAPGNLTENEIRGVNFNYADITTTLRRYDIKNMKNGYNKMPDGEEVY
                     FISTPSAGLWSTEEKLGSRV"
BASE COUNT          789 a          790 c          765 g          847 t
ORIGIN      
        1 cactatctcg tgcattatat cgcgtatcca cgggactatt ttttcaccct tctgtatcgg
       61 gtcgccgata ttttcccagg tgatgttcag gcccatatcc tcgaagcgtc gggcgacctg
      121 tacgatttcg cgtatggcgt aggtcagaga gcctgcgccc atatgaagta tgtttcgtct
      181 catttttttg gccttttccg tcaatattct aattcggctg aagcgaatgg tgtcaaaata
      241 tcgccagcct gacggacact tttgggctat catacaattt tgtgacgaca ttgcaaccgc
      301 tttgccgaaa attcaatggt tgccgccatc gggttcagaa tgtataatca cagtccagcc
      361 atgatgatag cgaaggaaaa agctgttatc gtcaatgctg acgacttcgg gcttagcgag
      421 ggcatcagtg aaggtatcct gcgcgcggcg acagagggta ttttaacgtc cacgactatt
      481 caggctaatc agccgctttg tgaaaaatgt ctgcgttttc tcaaaagcgc tccgacgcat
      541 ctcggcgtcg gcgtgcatct gaccataaac ggcggtcgtc cgctcagcat tgccggccaa
      601 gcccttgcgg atgagaacgg caaactttcc ctgacggcat cgcagacgtt tttgacgctt
      661 tttaaaaaac cttcactgct cgctgctgcg gaagctgaat tcgaagccca gattacatgg
      721 gcactcgacc acggaataaa acctacccat ctggacagcc atcgtcattc gcatgcatat
      781 ccgccgctgt tcaagcgtgt cgtgagactg gcaaaaaagt acaatattcc atttatacgt
      841 cagtacggtg aaaaatttat tggcgaagga tggcctggat acgaccctcg caatcttaag
      901 gcacacagaa tattgaactt ttttggctgg tacgtcgctc atcgctggcc ttccatgcgc
      961 gggactatcg gcacgtgggg agtgttgcag actggacgca tgtcggctga atggctggtt
     1021 cgcgcagcgc aggctgcgac gcaggttgac ggcgtggtgg aaataatgac tcatcccggc
     1081 ctgacgtacg accttacgcc gcagataaca cgcatgcgcg acagccgccg tgaagaacta
     1141 gacgctctta caaatccggc ggtaaaagaa gcctttcatc aatgcggcat aaaactcatc
     1201 aactatcgcg accttttaaa ttttgttgca acgtaataaa gcaggtttca ccttgcagca
     1261 gttgtatgtc agcttttgac tcaaaaagct gtagtaaaat tgtaaaaaat tctaaaacat
     1321 tcagaaaata gcaaaaactt gcgggttgat aaaattctgt ttgcaactta caattccgtc
     1381 atgaaaaatg aagatacatt cgcaccgctt gatgtagctc gtcagttgct ggcaatagaa
     1441 tcactgctcg gcagtctgga ttttcttcca gccagccgta atccgctaaa aatgcccaaa
     1501 ttgcctgcgc cggatatttt tgtccagcag gcaaacgagg cgaaattttc ctcttccgcc
     1561 gtaattattg atgaaatacc ggttcatctc acggttgaag agaaaatcgc ccgacttgcc
     1621 gaattggaag ttgaggtcgg gaaatgtacc ggctgcgagt tgtgcaaaac gcgcaacaag
     1681 gttgttttcg gtgagcggaa tccggcggcg cggctggtat ttgtcggcga agcgcctggt
     1741 gccgatgaag acgcacaagg ccggccattc gtaggccgtg ccggcgagct attgacaaag
     1801 atgataaacg ccatgggcct tcgtcgcgaa gacgttttta tatgcaacat gctcaaatgc
     1861 cgtccgccgc agaaccgcac acccaatccg caggagatac aggcttgctg gagtttcctc
     1921 gtgcgacagc ttcagataat caggccggaa gtcatagtga ctttgggcaa tccggcgacg
     1981 cagggtctgc tcaataccaa gatgggaata acgcggttac gcggcgaatg gcaggcgttg
     2041 ccggaaatag cgccaggcct cggcggcata ccggtaatgc cgaccttcca tccagcctat
     2101 gtattgcgga attattccgt cgatacgcgc aaaaaagtct gggcggattt gcaacaggtt
     2161 atggcgaggc tgaacctgaa aactcccaaa aaacagtgaa ttaactcaaa gtgaccgatt
     2221 gacgatattt tgttcagtaa atacttgttt tacaaaaagt tacacaaatt gtcaatttga
     2281 caaaaacaga ttgacaaatg attgccgtta aatcgggctg tttgaagaat gaattattcg
     2341 aacgcttcct caccgcagcc gacggctagc tgcgcgtgca aaacaccttt ttcgcggcgt
     2401 aagtgatttg ccagtgattg tatttctttt cccgtcccgc gaaccattat catttccgcg
     2461 cagatgtgct cttcgagatg cacatgcgta tttgcgagta cctttccact aaaatgatgc
     2521 tgtatgtcta gcagccgctc gaccagtccc ctggtatgat gattatatgt aatcgttatc
     2581 gtgccaagct gctgtttcct tgactgccag ttctgctcga ttatgctttc gcgtatcagg
     2641 tcgcggatat actccgagcg gttttcgtac tctgcctttc gtacgagttt ttcgaattta
     2701 tcgaatagcg gccgttcaat cgacacactg aatcgtacaa gttccgacat gaaattttcc
     2761 tcttttttct gtctgtcctg cttataatac gcaacattaa gcaagtgctg tccaataaac
     2821 tttcaaaccc ggctacccaa tttttcctcc gtagaccaca ggcccgctga tggagtgctg
     2881 ataaaatata cttcttcgcc atccggcatc ttgttatacc cgtttttcat attttttatg
     2941 tcatagcggc gtagcgttgt cgtaatatcg gcgtaattga agtttacgcc gcgaatttca
     3001 ttttccgtca gattaccagg tgcgtacgta accgtgaatc tgccgtcggt tgaaccgtgt
     3061 atgagatgag cggtcgcgtg tgtgaggtct ttcaaatctc cgccatccgg acatgcccgc
     3121 caacgagcca ttatattctc cgtgccggaa taaccgtatt gtctgattat cgcgtcgact
     3181 tcagcctgtt c
//
LOCUS       DAONFI010000280         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38007_length_3177_cov_3.200833, whole genome shotgun sequence.
ACCESSION   DAONFI010000280 DAONFI010000000
VERSION     DAONFI010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38007_length_3177_cov_3.200833"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1184)
                     /locus_tag="PLK08_08560"
     CDS             complement(291..1184)
                     /locus_tag="PLK08_08560"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPS53591.1"
                     /translation="MSIHDRQYTQDDYGGGMPTHGNMMWGLPRLTPAVKWLLIINAAT
                     FIVQYFLDGNMLISTLFGVSVERFWAFWTYLTFQFLHGGVWHILLNMLGVYFLGTTLE
                     NLWGSKPFLRFYLGCGAAAGLLYVIIGAITQTNPAAILIGASGGVFGLLLACAVYCPQ
                     FRLVFFVFPVPIRPAAVVIFGLMLISVLNAAVNGAIDSVMSNVAHLGGAFAAAFRIWA
                     WPVMREKKSDSRLNGSNKGAWKRRMAAAAGEEREVDRILQKIQREGIGSLNDKERKTL
                     QNATKRQRETDRRINDTFKNR"
     gene            complement(1181..1720)
                     /locus_tag="PLK08_08565"
     CDS             complement(1181..1720)
                     /locus_tag="PLK08_08565"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPS53592.1"
                     /translation="MSAKIIKEERLHKSFHFNLDRLTLQTHAGQEINRDVIRHPGAAL
                     ILPILTDGSMVLIRNYRYPAADYLVELPCGTIDNGEPPIECARRELLEETGYTAEIFE
                     ELASWYSCPGYSDEVIYAFVASGLKRGSQKLEEYEDISVIIRSDAEVRNMLADNQIRD
                     AKTISALGTYWIRKELSKR"
     gene            complement(1814..2713)
                     /locus_tag="PLK08_08570"
     CDS             complement(1814..2713)
                     /locus_tag="PLK08_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPS53593.1"
                     /translation="MKELVVISGKGGTGKTSIVASFAAMADEAVLADCDVDAADLHLV
                     LEPQIIRREEFYGGRREEINPAKCTGCGRCAELCRFDAVSAGRPEISHPIHTGYFSID
                     PISCEGCGVCAWFCPAEAIDFSPAINGEWYVSQTRFGPMVHAKLGIGEENSGKLVSTV
                     RREAHSIADERGMKLVIIDGSPGIGCPVVASITAADTVLIVTEPTLSGQHDMARVAEL
                     TRHFGIKTLLCVNKWNINPKITDEIESHARNNSLTVVGRIRYDKSVTAAQLCRKAVVE
                     YTPAGCSEDIKNLWAVVAESMKM"
     gene            complement(2710..>3177)
                     /locus_tag="PLK08_08575"
     CDS             complement(2710..>3177)
                     /locus_tag="PLK08_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HPS53594.1"
                     /translation="TAGKIDFVKGLLNVGEVMSPPVIRCVKAAAPNAELIILDCPPGT
                     SCPVIEAIRDSDFVLLVTEPTPFGLNDLRLAVETVRKLKLPFGVIINRADCGDNCTKK
                     YCDDESIAILAEIPDVREVAEAYSRGIMAAEVSQHYRKCFEKLLTALIKDEQR"
BASE COUNT          704 a          852 c          799 g          822 t
ORIGIN      
        1 ctggccgatt ttccgttttt ttcgcgtata aagctcaaag accacttttg ttataaacgc
       61 tattgaaatt ctcagacgca gaagacaaag aaatctttat tgagccatat aacgcgaatc
      121 ggccattttg aatcacaaca taaattacat tataaataaa caaatcccgc cgttcggcga
      181 gaaaacattc cggaactata aaatttacag tatcacagcg gaattatcaa gccttttaac
      241 ctcttttcga tgcctgaaaa attttaaccg gcgaattcct gatgcctgat ttatcgattc
      301 ttgaacgtgt cgtttatgcg gcggtctgtt tcgcgttgac gtttggtggc attctgaagg
      361 gttttgcgtt ctttgtcgtt caatgaacct ataccttccc tctgtatttt ctggagtatc
      421 cggtctactt cgcgttcttc cccggcggct gcggccattc gacgtttcca tgcgccttta
      481 ttgcttccat tcaaacggct gtcggatttt ttttcgcgca tgaccggcca ggcccatatc
      541 ctgaatgccg ccgcaaacgc tccgcccaga tgtgcgacgt tgctcattac ggaatcgatg
      601 gcgccgttga cggcggcgtt cagtacgctt atcagcataa gcccgaagat taccaccgct
      661 gccggccgta tcggaacggg aaatacgaaa aaaaccagcc tgaattgcgg acaataaacc
      721 gcgcatgcca acagcaatcc gaatacgccg cccgacgcgc ctatcagaat cgccgcagga
      781 tttgtctgcg tgattgcacc gattattacg tacagcaggc cagccgccgc gccgcagccg
      841 agataaaatc gcaaaaacgg cttactgccc cacaggttct caagcgtagt gcccagaaaa
      901 taaacgccca acatgttcag cagaatgtgc cacacgccgc catgcaggaa ctgaaacgtc
      961 agatatgtcc agaacgccca gaaccgctca acgcttacgc cgaacagcgt gctgatgagc
     1021 atgttcccgt ctaaaaaata ctggacgatg aacgtcgcgg cgttgatgat gagcagccat
     1081 ttgactgcgg gcgtaagcct cggcagcccc cacatcatat tgccgtgcgt cggcatgccg
     1141 ccgccgtaat cgtcctgcgt gtattgccgg tcgtgaatgc tcatcgtttg gacaattcct
     1201 tccttatcca gtacgttccc aatgcggaaa tggttttggc atcgcgtatc tgattgtcgg
     1261 caagcatgtt gcgaacttca gcgtcgctgc gtatgattac ggagatatcc tcgtattctt
     1321 cgagtttctg gctgccgcgt ttaaggccgc tcgccacgaa ggcgtatatg acttcgtcgc
     1381 tgtagccggg gcatgaatac cagcttgcga gttcttcaaa aatttctgcc gtgtagccgg
     1441 tttcttcgag cagttcgcgg cgtgcgcatt ctatcggcgg ttctccgttg tcgatagtgc
     1501 cgcagggcag ttccaccagg taatcagccg ccggatagcg atagttgcgt attaaaacca
     1561 tgctgccgtc ggttaatatt ggcaggataa gcgcggcgcc tggatgacgg atgacgtcgc
     1621 gatttatctc ctggccggcg tgagtctgca gcgtcaacct gtcaagattg aagtgaaaac
     1681 ttttatgcag ccgctcttcc ttgattattt ttgcactcat gccgggaatt ataacaaaac
     1741 gctgcgaaag cgtaaacccg tcagccggca caaatgaaac gaataacaga ttttttgaag
     1801 aaaaaccccc atgttacatt ttcattgatt ctgcgacgac ggcccataaa ttttttatat
     1861 cttccgaaca gccggccggc gtatattcca ctaccgcttt tctgcacagt tgggctgctg
     1921 tgacggattt gtcgtagcgt atgcggccga cgaccgttag gctgttattt cgggcgtgtg
     1981 attcaatttc gtctgttatt ttcgggttta tgttccattt gttgacgcac aggagcgttt
     2041 taattccgaa gtgtctggtc agttcggcta cgcgggccat gtcgtgttgg ccgctgaggg
     2101 tgggttcggt tacgatgagt accgtgtcgg cggctgttat tgaagcgaca accggacagc
     2161 ctatgcccgg cgaaccgtct ataatgacca gtttcatgcc tcgctcatct gcaatgctgt
     2221 gagcctctcg ccgtaccgta cttacgagct tgccggagtt ttcttccccg atgccgagtt
     2281 ttgcgtgaac catcggcccg aatcgcgtct gcgagacata ccattcgccg ttgatagctg
     2341 gcgaaaaatc aatcgcctcc gccggacaga accacgcaca gaccccgcag ccttcgcatg
     2401 aaattgggtc tatggaaaaa taccccgtgt gtatgggatg agaaatttcg ggcctgccgg
     2461 cggagacggc atcgaatcga cacagttccg cacatctgcc gcagccggtg catttggccg
     2521 gatttatttc ctcccgcctg ccgccgtaaa attcttcgcg acggattatc tgcggttcga
     2581 gtacgagatg caagtcggcg gcatcgacgt cgcaatcagc cagcaccgcc tcgtccgcca
     2641 ttgctgcgaa cgaggcgact atggacgtct tgcccgtgcc gcctttgccg ctgattacga
     2701 ccagttcttt catcgctgtt cgtcctttat cagagccgtc agcagttttt cgaaacattt
     2761 tcggtaatgc tgcgatactt cggcagccat gattccgcgg gaatatgctt ccgccacttc
     2821 acgtacgtcc ggaatttcag cgagtatcgc gatggactca tcgtcgcaat atttttttgt
     2881 gcaattatcg ccgcagtccg cgcgattgat tatgacgccg aagggcagtt tgagttttcg
     2941 cactgtttcg accgccagcc gcaggtcatt caggccgaaa ggagtcggtt cggttaccag
     3001 caatacgaaa tcgctgtcgc ggatagcttc aataacaggg catgacgtgc ctggcggaca
     3061 gtccaggatg ataagttcgg cgttcggggc tgcagccttt acgcatcgta tgacaggcgg
     3121 gctcatgact tcgccgacat taagaaggcc tttgacgaaa tctatcttgc cggctgt
//
LOCUS       DAONFI010000281         3173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38069_length_3173_cov_3.609044, whole genome shotgun sequence.
ACCESSION   DAONFI010000281 DAONFI010000000
VERSION     DAONFI010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38069_length_3173_cov_3.609044"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLK08_08580"
     CDS             <1..1215
                     /locus_tag="PLK08_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53595.1"
                     /translation="ELITSRLGGYIKKCDIVSANISDGITTVAIRALVDRDTAGDDIS
                     AIQYAARTADNPRIAVVVAQITNDLFEDMVKNKTVERDTQVKINVERRRLEDAKAASL
                     HELDEGGTRVSGNFGFWNRRPRPRNWTTWTAQERSAWITQTAENEYRNAVAEISNSES
                     FRKQKSVWSKVTDSVQQDGMVQSVIEKYFVEQGLNLVSQSQIDIARQREMMLAGLSGN
                     PDEMTAVRASLGADILITGTATASRVSEIKVGDAKLYRYNGEIVVRAYNVDNGRLLAV
                     ENFGQVVNSTLPTGGADDALRTVARTAAISLADSIANIWTRQWQSGRAITLSVSGMTF
                     DDWQKFKAQVQNWPRVTQVRLRSIAAGRADIEITTELGGEYVASRIAGMKTPAIEVTG
                     FEQNNIRGKIRR"
     gene            complement(1255..2658)
                     /locus_tag="PLK08_08585"
     CDS             complement(1255..2658)
                     /locus_tag="PLK08_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53596.1"
                     /translation="MRSASTALTYIWLAAAGFIVGCEPEKIDAYRNPLDVMEKKLPAS
                     AGKSDYILARMEYGQSAMDLGCYLRAEPALEEARQQFSADKSNIAATVSSEKHKFYKG
                     ETYERACLCNYLGYIKYLNGDFNNARIYFSNAVNEDRKAVGENGNPLYGDDFSLSYYW
                     LGRAYMKLGDGDNAAIAMKKAARESVREQADLDNEKKDDQNFFEKKYKKMLDGEEWCW
                     KTFNNPEKPELVVKDMINLTKVSQNISQADVVQKNAAAKSPLRTAAKTRDEFFTTDFQ
                     NKANLIMTIEVGNPPYKTLAGAKNELTQINKLHIQPFYAYVYVDGHPLGKAFRVLELW
                     DQATTQSRVGAKDAAQVTKAVLMTVAEQLIGTSWDVSGDVRYWHSLPGRVFISAVDAE
                     PGIHTVQIDFYDINGKFLPRWTNVYPGVKVTDKKETLMLLRPTFDGNNSITPIEYANA
                     RKAGANPYGWKYPDKPE"
     gene            complement(2686..>3173)
                     /locus_tag="PLK08_08590"
     CDS             complement(2686..>3173)
                     /locus_tag="PLK08_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPS53597.1"
                     /translation="LMLGGLSSKDFRGVCFKMTQSLITLPQIQNAQRPPKIAFAKVTN
                     KSDEIIDTQGFLEKMRTELIKNSNGKVVFIDRENIGQIMDERDMKDAGQFTGETTKKM
                     LGADYLLAGTITGITSARGRKSTNYMRLAFRLTDANTGVIIWEDDYEMKYYHKAGLYD
                     R"
BASE COUNT          716 a          775 c          840 g          842 t
ORIGIN      
        1 gaactgataa cgtcgcgtct tggcggatat attaaaaagt gcgatatcgt ttctgcaaac
       61 atttctgacg gcattaccac cgtcgccatc cgcgcacttg ttgacaggga caccgccggt
      121 gacgatatct ccgccattca atatgccgcc agaactgccg ataatccccg aatagcagtg
      181 gtcgtcgcgc agattacgaa cgacctcttc gaggatatgg taaagaacaa aacggttgaa
      241 cgcgatacgc aggtgaaaat caatgttgaa aggcgtcggc tggaagatgc caaggctgcg
      301 tcactgcacg aactcgacga aggcggcacg cgggtgagtg ggaatttcgg gttttggaat
      361 cgccggccca ggccacgcaa ctggactaca tggactgcac aggaacgctc cgcatggata
      421 acgcaaactg cggaaaatga atatcgcaac gcggtagctg aaatcagcaa ttcggaatcg
      481 ttcaggaaac agaaatcggt atggagtaaa gttacggact ccgtgcagca ggatggcatg
      541 gttcagtctg tgattgaaaa atattttgta gaacaggggc tcaacctcgt cagtcagtcg
      601 cagatagata tcgcacggca gcgtgagatg atgttagccg gccttagtgg aaaccccgat
      661 gaaatgaccg ccgttcgcgc ctcgctcgga gcggacatac tcataaccgg aacagcgacg
      721 gccagtcgtg tttcagaaat aaaggtcggc gacgcgaagc tgtatcgcta taacggtgag
      781 attgtggttc gtgcgtacaa tgtcgataat ggccgactgc tggcggtgga aaatttcggc
      841 caggtggtca attccacgtt gcctaccggc ggtgctgatg atgcgttgcg gactgttgcc
      901 agaacggctg ctatctcatt agccgacagt attgcgaata tatggactcg ccaatggcag
      961 agcggccgtg cgattacgct gagtgtatcg ggcatgactt ttgatgattg gcagaaattc
     1021 aaggctcagg ttcaaaattg gccgcgtgtg acgcaggtac ggctgcgcag catcgcggct
     1081 ggcagggctg atattgaaat aactactgaa ctgggcggcg aatatgtcgc cagccgaatt
     1141 gccggaatga aaacgccggc aattgaagtc acaggttttg aacaaaacaa tattcgcggt
     1201 aaaattcgcc gataattttc agagtggctc tcttcacaaa cactctatca gcaattattc
     1261 cggtttgtca ggatacttcc agccatacgg attcgcgccg gctttgcgtg catttgcata
     1321 ttcgattggc gtgatgctgt tattgccgtc aaacgtcggc ctcaacagca tgagcgtctc
     1381 ttttttgtcc gtcaccttta ctccagggta gacatttgtc caccgcggca gaaacttacc
     1441 gttgatgtcg taaaaatcta tctgtaccgt gtggatgccg ggttccgcat cgacagccga
     1501 tatgaatacc cgccctggca gtgaatgcca gtaccgcaca tcgccgctga cgtcccacga
     1561 agtaccgata agctgttccg ctaccgtcat aagtacggct ttcgtaacct gagctgcgtc
     1621 ttttgcgccg actctcgatt gcgtggtagc ctggtcccac agttcaagta cgcgaaaggc
     1681 tttgcccagc ggatggccgt cgacgtatac atatgcataa aacggctgaa tatggagctt
     1741 attgatttgg gtaagttcat ttttcgcacc ggcgagagtt ttgtatggcg gattgccgac
     1801 ctcgatggtc ataatcagat tagccttgtt ctggaaatcg gtcgtgaaaa attcatcacg
     1861 ggtttttgcc gcggttctga ggggtgattt tgcggcggcg tttttctgaa cgacgtcagc
     1921 ctgcgagata ttctgcgaaa ctttagtaag attaatcatg tctttgacca caagttcagg
     1981 cttttcggga ttattgaaag ttttccagca ccactcttcg ccgtcgagca tttttttgta
     2041 tttcttttca aaaaaattct ggtcgtcttt cttttcgtta tcgaggtcag cctgctctcg
     2101 cactgattca cgtgcagcct ttttcatggc gatggccgcg ttgtcgccgt cgccgagttt
     2161 catgtatgcc cggcccagcc agtagtatga caacgaaaaa tcatcgccgt atagcggatt
     2221 gccgttttcg ccgacggctt tgcggtcttc atttacggcg ttgctgaaat agatgcgtgc
     2281 gttgttgaaa tcgccgttca gatattttat gtagcccaga taattgcaga gacatgcacg
     2341 ttcgtatgtt tcgcccttgt agaatttgtg tttttcagag ctgacggttg cggcgatatt
     2401 ggacttgtcg gcggagaact gttgtcgcgc ttcttccagc gcgggttcag ctctcagata
     2461 gcagcccagg tccatggcgg attggccgta ttccattctc gccagtatgt aatccgactt
     2521 gccggctgac gcgggcagtt tcttctccat gacgtcaagc ggattacggt aagcgtcgat
     2581 tttctccggt tcgcagccga caataaaacc tgccgcagct aaccatatgt atgtcaatgc
     2641 ggtcgatgct gaacgcatga aactctcctt gtatacgtga ggggattatc ggtcgtagag
     2701 gccggcttta tggtaatact tcatttcgta gtcatcttcc catattatta cgccggtgtt
     2761 ggcatcggtc agccgaaaag ccagacgcat ataattggtc gacttacggc ctctcgcact
     2821 ggttatgccc gtaatcgtgc cggccaacag gtagtcggct cctagcatct tcttggtagt
     2881 ctcgccggtg aattggccgg catccttcat gtcacgctcg tccataatct ggccgatgtt
     2941 ttccctgtct atgaacacga ccttgccgtt ggagttcttt atgagctcgg ttcgcatttt
     3001 ttccaaaaat ccctgcgtgt cgataatttc atcgcttttg ttcgtcactt tcgcgaaagc
     3061 gattttcggc ggacgctggg cgttctgtat ctgcggaagt gttatgagcg actgggtcat
     3121 tttgaagcat acgccgcgaa aatccttgct gctaagcccg ccgagcatca ggt
//
LOCUS       DAONFI010000282         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38161_length_3168_cov_4.251526, whole genome shotgun sequence.
ACCESSION   DAONFI010000282 DAONFI010000000
VERSION     DAONFI010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38161_length_3168_cov_4.251526"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="PLK08_08595"
     CDS             complement(<1..1744)
                     /locus_tag="PLK08_08595"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPS53598.1"
                     /translation="MTLKLRQKIILLALIAGLLPVLVIVVGSYRIKMKTGATLINELT
                     VLSKATVTQVAGDVYALCETVNNMIQSNLHDYLIITEKTIEHDGGLALSPEKTVTWTL
                     INKDTDKTETITLPQMMLGDTPIEPVYDLADDSPQPFIHIVDGMRVLGGTCTIFQRMN
                     EEGDMLRIATSVVNADGRRSIGTYIPHTSEVVKKVLAGQMAIGRFMVLNKWVMASYKP
                     YYRDGRIIGMLYCGMAIDEIKSIEKEISGIKFGKSGYVTVILGEGPERGKYIIAPQDK
                     NLIGKNIFHEHSGNNNYFNQLISRAISSEPGKVIEGGYTIKLANFSSPRENIAAVVFY
                     KPWNWIIVAGTFLDELNDTRASAEHSIQIVLLRFVAVGLALFILILIAIFFFSGTISK
                     PISRVAAITRNVSAGNISLAADLTEEYFSDYIKQPESARRDETSQLIYAVSEMTLNLN
                     ELVGQVQHSSVQLLSNATQVAATAKQQEGSLSELGSSTSEIAASIKQISATSSDLAGT
                     MNKVTAVADETTSLADDGRQGLVQMEDQMRELATATSSISSKLALINDKTANISNVVT
                     TINKIAEQTNLLSLN"
     gene            complement(2013..3017)
                     /locus_tag="PLK08_08600"
     CDS             complement(2013..3017)
                     /locus_tag="PLK08_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53599.1"
                     /translation="MAKSALQKAILVGIIIAAVLAFLTPAFMLLSGLFAPNSTGQLVL
                     WSVGMFCSYLLGLTGIFFLVLACIALASNQKLLYSSVTTTNRLESLLEKQSETFRMLI
                     NLSQLSDKVKSLIYREQEVEALYESFNSFLFHQDYQSAENLVKQMEVGLGLVDEAARM
                     REEIEQNRRATIDDKLATAIEKIRDIIDKYDWPRAKREAARLGKLFPDNPKIAALPQD
                     IVKAFNVRKRDLLKEYEKAVKVNDVEKGIDLLKELDRYLTPEEGAALTESARDVFKKK
                     LHNLGVQFAIAIADQQWQVAVKTGEQIAQEYPNSRMAREVREKMDLLKQYAAAADVSV
                     "
BASE COUNT          678 a          782 c          820 g          888 t
ORIGIN      
        1 cgttcagact gagcagattg gtctgctcgg ctatcttgtt gatggtcgta acgacgttgc
       61 tgatgttagc cgtcttgtcg tttatcaagg ccagcttgga tgaaatcgac gacgtagccg
      121 tcgccaactc acgcatctgg tcttccatct gtaccaggcc ctgacggccg tcgtcggcta
      181 gcgaggtagt ctcgtcagcc accgccgtca ccttgttcat cgtgccggca agatcgctgc
      241 tggtggcgga tatctgtttt attgaagcgg ctatctcgga tgtggacgaa cccaattcac
      301 tcaaagagcc ttcctgctgt ttcgccgtcg cagccacctg cgtcgcattg ctcagcaact
      361 ggacgcttga gtgctgaacc tggccgacga gttcattcag atttagcgtc atttccgata
      421 cggcgtaaat aagttgcgac gtttcgtcac gcctggctga ttccggctgt ttgatgtaat
      481 ccgaaaaata ttcttcggta agatcggcgg ccaggctgat atttccggcg gagacattcc
      541 tggttatggc ggctacgcgt gatatgggtt tggatattgt tccgctgaag aagaatatcg
      601 caatcagaat caatatgaac agggcaaggc cgacggctac gaaacgtagc agaactatct
      661 ggatgctgtg ttcggctgag gcacgagtgt cattcaattc gtccaaaaat gtgccagcca
      721 cgattatcca gttccacggc ttgtaaaata cgactgccgc gatgttttct ctcggactgc
      781 tgaaattggc aagtttgatg gtataaccgc cctcgatgac tttgccaggc tcggatgata
      841 tggcccttga gataagctga ttgaaatagt tattattgcc gctgtgctcg tgaaagatat
      901 ttttgcctat cagattttta tcctgcggtg cgattatata cttgccacgt tccggccctt
      961 cgccgagtat tacggtcaca taaccgctct tgccgaattt gatgcccgaa atttcctttt
     1021 ctatggattt tatctcatcg atagccatcc cgcaataaag catgccgatg attctgccgt
     1081 cgcgatagta aggtttatac gacgccatca cccatttgtt cagaaccatg aatctgccga
     1141 tcgccatttg gccggcgagc acttttttta ccacttccga cgtgtgcggg atatatgtgc
     1201 cgatgctgcg acggccatcg gcattcacga cgctggtggc tatgcggagc atgtcgccct
     1261 cttcattcat acgctggaat atcgtgcacg ttccgcctaa aacccgcatg ccgtcgacta
     1321 tatggatgaa aggttgcggc gagtcatcgg ctaaatcgta gaccggttcg atgggggtat
     1381 cgcccagcat catctgcggc agggtgatgg tttcggtctt gtcggtgtcc ttattgatga
     1441 gagtccatgt gacggttttt tccggagaaa gagcaaggcc gccgtcatgt tcaatagttt
     1501 tctccgttat tatcagataa tcgtgaagat tactctgaat catgttgttt actgtttcgc
     1561 agagagcgta tacgtcgccg gcgacctggg tgacggtggc tttgcttagg acggtgagtt
     1621 cgtttatgag agttgcgccg gttttcatct ttatgcggta agagccgacc actatcacaa
     1681 gcaccggcag caggccggcg atgagtgcga gcaatatgat tttctgtctc agcttcaagg
     1741 tcattttccg tctcctgaaa tatatcggac tgccggtcgg caaattggcg gcattgaggc
     1801 agacaggacg atacaaatga caaccgccgc taaaagcaaa aattatttct taacattact
     1861 gcaatgcgca ttcgttgaag caacatatca taatatacgt aaacgtcggc tggattatgc
     1921 ggcacacgtt ttatccagcc ggcgtttttt aataccgcgg gttttagagc aagctctagc
     1981 ctgtagcagt ttattgaaat cgaaatcttt ccttatacgc tgacatctgc cgccgcggcg
     2041 tattgtttca ataaatccat tttttcccgc acctcgcgtg ccattctgct gtttggatat
     2101 tcctgtgcaa tctgttcgcc ggtctttacc gcaacctgcc actgctggtc ggcgatggcg
     2161 atggcgaact gtacgccgag gttatgcagt tttttcttaa aaacgtctct tgcggattcc
     2221 gtgagtgctg cgccttcttc cggcgtaagg taacggtcaa gttccttgag caggtcgatg
     2281 cctttttcca cgtcgttaac cttgacggct ttttcgtatt ccttgagcag gtcgcgtttc
     2341 cgcacgttga atgcttttac gatatcctgc ggcagagcgg cgatttttgg attatcgggg
     2401 aacagtttgc cgagccgggc ggcttcccgc ttggcacgcg gccagtcgta tttgtcgatt
     2461 atgtctctga ttttttcaat cgcggtagcc agtttgtcgt ctatggtcgc acggcggttc
     2521 tgttcgattt cctctcgcat gcgggctgct tcgtcgacga ggccaaggcc gacttccatc
     2581 tgctttacga gattttcggc agattgataa tcctggtgga acaggaacga gttgaaactt
     2641 tcataaaggg cttcgacttc ctgttcacgg tagatgagac ttttgacctt gtccgacaac
     2701 tgcgagagat tgattaacat acggaacgtt tccgactgtt tttcaagaag cgattccagc
     2761 ctgtttgtcg tcgttacgct ggaatatagc agtttctggt ttgacgccag cgcgatgcac
     2821 gccagcacga gaaaaaatat ccccgtcagt ccgagcagat aactgcagaa catgccgaca
     2881 gaccataaca cgagttggcc ggtcgaattt ggtgcaaaca gtccggaaag cagcatgaat
     2941 gccggcgtga gaaacgccag tacggctgca atgataatac caaccaggat agctttctgc
     3001 agagcacttt tggccatgat tgtctccttg ttctgccctc acctcggatt atcgcctgtc
     3061 gcatggggcg tcacgacggc ggcgtgtatt gacatttccg tgcaggttcg ccggagaagg
     3121 gcgtcttacg cagggttctt ctggctgccg ccgaaaatgt tggaaaat
//
LOCUS       DAONFI010000283         3166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38201_length_3166_cov_3.906140, whole genome shotgun sequence.
ACCESSION   DAONFI010000283 DAONFI010000000
VERSION     DAONFI010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38201_length_3166_cov_3.906140"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLK08_08605"
     CDS             <1..242
                     /locus_tag="PLK08_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53600.1"
                     /translation="QAAKEPIAGSQGFWDRFYCQFDNICPQKCHFGRNFPQNDSQKIL
                     IFNAWKSFSGRKTLWVLFWPCMLETLNRMKDLWNI"
     gene            239..688
                     /locus_tag="PLK08_08610"
     CDS             239..688
                     /locus_tag="PLK08_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HPS53601.1"
                     /translation="MKIRGHHLVCVYCFYGSGEKTAEAFFSLKNAIPELLLKLRQNPE
                     MEITVAANLDDVCDMCPLRKPNGCGRTSDPVRQNEKLCGWDRALLEVLGLHEGDRIKA
                     QKLEDRIRERIPDISKFCTNCTSASPSGWSEYKKAIREGLWPDKTKN"
     gene            complement(883..1371)
                     /locus_tag="PLK08_08615"
     CDS             complement(883..1371)
                     /locus_tag="PLK08_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPS53602.1"
                     /translation="MRPGWDEYFMEVADAIAKRATCDRGRSGCVIARDRQILVTGYVG
                     SPPGLPHCDEVGHQFKKVIHEDGSITEHCVRTVHAEQNAICQAARNGIALDGATLYCR
                     MTPCRQCAMLIISCGIKRVVCEYRYHAGEESEKLFRQAGVELQFMNPQILEYSRQKAP
                     EQ"
     gene            complement(1368..1676)
                     /locus_tag="PLK08_08620"
     CDS             complement(1368..1676)
                     /locus_tag="PLK08_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53603.1"
                     /translation="MTHRAKEKIGTIGTLVAAAILGAAAWEAVSGLVRPAIAQPAGST
                     MDDPRNATMFRLESMNQYKILNSEVRRIREILESGKLKVVADVPQLEQDADDSKRKSR
                     "
     gene            complement(1697..3133)
                     /locus_tag="PLK08_08625"
     CDS             complement(1697..3133)
                     /locus_tag="PLK08_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPS53604.1"
                     /translation="MIKTLDRYIIRTFLHSLLMWFIVVLVLRIVVDMFTNMDEFTEGD
                     PTVMELIKNVLIYYSTQMLVYIAEMGGIIIVASAAFTIARMQHTNELTAMLASGVSLY
                     RVVWPIICAAMIMGAIIVVDREVAMPSVRQYLVLKPDEIATMDNSRFSLSFIVDNDGT
                     CWNGQAYDSAKNTIEYPLVIMRDGQTYQYVAHASGRLAEPGMVNGQRGWFVTDGSLMK
                     MDIRTAHGGEAMKMWRQSQGSNAVYTTIGPEVLVKIAKRLFEQKNKIPFPANRAFTAV
                     SYIHDFDRHYNMELRAPRLELKNDSEGNIIAGRLESPQFEFYAEKKRLIAIITAKNAI
                     WIPGSAVQSRWQLNGGRIFICSDLTPDEVELKQSERWLNYLTSNEMTRMLQLKRATDP
                     SSIRMMKFNRVVEPFNSLVMLLLGLPFILSRQRNIKASALRCVLVVGLFYVFIQFCQY
                     VLPDAWAAFMPLMIFGPISILMLDSIKT"
BASE COUNT          777 a          793 c          784 g          812 t
ORIGIN      
        1 gccaggctgc aaaagagccg attgcaggca gccaaggttt ttgggataga ttctactgtc
       61 aatttgataa catttgtccc caaaaatgcc atttcggcag aaattttcca cagaacgact
      121 ctcaaaaaat cctcattttt aacgcctgga aaagtttttc tggtagaaaa acgctatggg
      181 tgttattctg gccatgcatg ctggaaacac ttaatcggat gaaggactta tggaatatat
      241 gaaaatcaga ggccaccatc tcgtttgcgt gtactgtttt tacggctctg gcgaaaaaac
      301 agcggaagcg ttcttttcat tgaaaaatgc cattccggaa ttgttgttaa aattgcggca
      361 gaatccggaa atggaaatta ccgtggctgc gaatcttgat gatgtatgcg atatgtgtcc
      421 gctcagaaaa ccgaatgggt gcggacgcac ttccgaccct gtccggcaaa atgagaaact
      481 ttgcggctgg gacagggcgt tgctggaggt gcttggcctt cacgaaggcg accgcatcaa
      541 agcccaaaaa ctcgaagacc gtattcgtga aagaatacct gatataagca agttctgtac
      601 aaattgtaca agtgcttcgc caagcgggtg gagtgaatat aaaaaagcaa ttcgtgaagg
      661 tttatggccg gataagacaa aaaattaatt ttgagaatcg cgataatata tgtcactgaa
      721 cgaagtgaag agcctgacgg caattaaggt tcaatagaca aaaccaattt ataagcaact
      781 tcgaatatgc aagttttctg aaacaccttt ttgaaaaatc gcagtggttt tcaaaaacat
      841 gcatggatag tttttcccgc ttttgcgtag taaatttatc gttcactgtt ccggagcttt
      901 ttgtcgcgaa tattccagaa tctgcgggtt catgaattgc agttccacac cagcttgccg
      961 gaagagtttt tccgattctt cgccggcatg gtaacgatac tcgcacacca ccctttttat
     1021 gccgcaggat attatcagca tggcgcattg cctgcagggt gtcattcggc agtaaagcgt
     1081 cgcgccgtcg agtgcaatgc cgtttctggc cgcctggcag attgcgtttt gctcggcatg
     1141 gacggtacgc acgcaatgct cggtaatgct gccgtcttca tggatgactt tcttgaactg
     1201 gtggccgact tcatcacaat gcggcagccc tggcggcgaa ccgacataac ccgtcaccag
     1261 tatctgccgg tcacgcgcaa taacgcagcc gcttcggccc ctgtcgcacg tggcgcgctt
     1321 ggcgatggcg tccgccacct ccatgaaata ttcgtcccag ccgggtctca tctggatttt
     1381 ctcttcgaat cgtcggcgtc ctgttcaagc tgtggaacat cggcaacgac cttgagtttg
     1441 ccgctctcca ggatttcacg gatgcggcga acctcactat tgagaatttt gtactgattc
     1501 atgctttcaa ggcgaaacat cgtagcgttt cgcgggtcgt ccattgtcga gcctgccggt
     1561 tgcgcaatcg ccggccggac aagaccagaa actgcttccc acgccgccgc accgagaatc
     1621 gcagccgcca ccagcgtgcc gattgttccg attttctctt tagcccgatg agtcattttg
     1681 tacacctccc accagttcag gtttttatgc tgtccagcat cagtatggaa ataggcccga
     1741 aaatcatcag gggcatgaac gccgcccacg cgtcgggcag cacgtattga cagaattgaa
     1801 tgaaaacgta gaacaggcct actacgagca cgcaacgcag tgcggaagct tttatgttcc
     1861 gctgccgcga aagaataaac ggcaggccca gcaaaagcat aacaaggctg ttgaacggtt
     1921 cgacaacgcg attgaatttc atcattcgta tgctgctggg gtcagtggcg cgttttagct
     1981 gcagcatccg cgtcatttcg ttgctggtga gatagttcag ccatcgctcg gactgtttca
     2041 attcgacttc atccggcgtt aaatctgaac atatgaatat tctgccgccg ttgagctgcc
     2101 acctggactg tacggctgag ccgggtatcc agattgcatt tttggcggtt attatggcga
     2161 tgagtcgctt tttttctgca taaaactcga attgcggcga ttcaagccgg ccggctatta
     2221 tattgccttc cgaatcgttt ttaagctcca gccgcggcgc gcgaagttcc atattgtaat
     2281 gacggtcaaa gtcatgaata taactcacgg cagtaaacgc gcggttagcc ggaaacggta
     2341 ttttattttt ctgctcgaaa agccgcttgg cgattttaac gagcacttcc ggccctatgg
     2401 tcgtataaac ggcattgctg ccctgagact gtcgccacat tttcatcgct tcgccgccgt
     2461 gagccgtcct gatgtccatc ttcatcagcg agccgtccgt aacaaaccag ccgcgctggc
     2521 cgttcaccat gcccggctca gccagccggc cggatgcatg cgcaacatat tgatacgtct
     2581 ggccgtcgcg cataatcacg agcggatact caatggtgtt tttggcggaa tcgtatgcct
     2641 ggccgttcca gcatgtgccg tcgttatcga ctatgaagct cagcgaaaaa cgggaattgt
     2701 ccatcgttgc gatttcgtcc ggttttaaaa ccagatattg tcgcacggac ggcatggcta
     2761 cctcgcggtc gacgacgatt atcgcgccca taatcatggc tgcgcagatg ataggccaga
     2821 ccacgcgata aaggcttaca cccgacgcca gcatggcggt aagttcgttg gtgtgctgca
     2881 tgcgagcaat ggtgaacgcg gcggaggcga ctattattat gccgcccatt tcggcaatgt
     2941 acacaagcat ctgcgtcgaa taatatatca gaacattttt gatgagttcc atgactgtcg
     3001 ggtcgccttc cgtgaattca tccatgttcg taaacatgtc gacgacaatt cgcagaacca
     3061 gtacgacgat gaaccacatc agcagcgaat gcagaaacgt gcgaattata tatctgtcca
     3121 gcgtttttat catggtttta tctcctgctt atcctgagat aaacca
//
LOCUS       DAONFI010000284         3156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38379_length_3156_cov_3.786198, whole genome shotgun sequence.
ACCESSION   DAONFI010000284 DAONFI010000000
VERSION     DAONFI010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38379_length_3156_cov_3.786198"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLK08_08630"
     CDS             <1..230
                     /locus_tag="PLK08_08630"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HPS53605.1"
                     /translation="SRILRLEEKLAFQEKTIADLDEVVTAINLKFSELTRGFEDITKM
                     VRQLDADRAERHRNGDEKPPHYGGLGSAAHK"
     gene            399..1514
                     /locus_tag="PLK08_08635"
     CDS             399..1514
                     /locus_tag="PLK08_08635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPS53606.1"
                     /translation="MADNPSNTRYGGQRQIPGYQLLEKLGSGAMAVVYKARQISLDRV
                     VAIKILSSRLSKDEEYVKRFYAEGQIAAKLNHPNIVQSIDVGQAGEYYYFVMEYVEGK
                     TVYEELAAGKKYAEAEALDIAIQMAKALQHAHEQGLIHRDVKPKNIMITPGGVAKLMD
                     MGLARVADDAKAIESEAGRLFGTPYYISPEQILGRKDIDFRCDIYSLGATLYHMLTGR
                     PPFQGKTSKEVMIKHIKKAPRSPDRYNMELSRGICAVVEKMLAKNPAHRQSSTKELVE
                     ELQSVEFLLDTSDSADTAAGIENIADNIRKIAEIADKKSSVHKPDPGKNSLKTPLKNE
                     QPSVIRVAERNPLNKLLLAGFIASLILNLILLLLWLK"
     gene            1652..1894
                     /locus_tag="PLK08_08640"
     CDS             1652..1894
                     /locus_tag="PLK08_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53607.1"
                     /translation="MDIHNIAIKKPQRTYVLRYAEGSEEQAVEEVMRWADDPSHEIDW
                     QDAADISWRILNSVAADINRIAEDSAHHALKGGSQV"
     gene            1891..2868
                     /gene="xerC"
                     /locus_tag="PLK08_08645"
     CDS             1891..2868
                     /gene="xerC"
                     /locus_tag="PLK08_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPS53608.1"
                     /translation="MIITLTNEFLDYIQVKRNFSAHTIRSYAADLMQFCQYLAAAGSG
                     DPLQLGETATSLDKLPPLEEADVAAVEARILSVEPVEIRAYLGVLSENKYSRSTIARK
                     LATLRSFYKYLVRTGRLESSPVSVIRTPRQDRRLPKFLDISQIDRLLDAPDKNTLLGA
                     RDSAILETIYSAGLRISELVGLNIEDMDENEGVLRIRGKGRKERLALLGSKAVDALRH
                     YRRKRDAALGPTNQGAMFLNRDGTRISDRSIRRKLDKYMAIAGLPSHISPHTLRHTFA
                     THMLNAGADLRCVQEMLGHENLSTTQIYTHLTTRQLRQVYDKAHPLMKS"
     gene            2881..>3156
                     /locus_tag="PLK08_08650"
     CDS             2881..>3156
                     /locus_tag="PLK08_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53609.1"
                     /translation="MMMIIWCFCIYFTGRGLYRMWSSVIGGAVTDWLMLPATLLSELL
                     YSGGRLMTGRPVYAGIIAPQNVTADPCRVAISGKNGFWVEMLSAGLAM"
BASE COUNT          790 a          769 c          871 g          726 t
ORIGIN      
        1 aatcgcgaat actcaggctt gaggagaaac tggcgtttca ggaaaagacc atcgccgacc
       61 ttgacgaggt cgtcacggcg attaatctga aattttcaga gctgacgcgc gggtttgagg
      121 acattacaaa aatggtgcgg cagttggatg ctgacagggc tgagcggcat cggaacggcg
      181 acgaaaagcc gccgcattac ggcggtttgg gctcggcagc tcataaatga cagcccaaat
      241 tgtgcagagc gatgcaatgt tgaacgcctg cgatagaaat gacatgacga tattttcttt
      301 ttaatctgtg tgaaatggaa tgtttatcat gccggcaagc cgtaagaggc ttggcttttt
      361 tgaattatgg ttttgattta ttgttttaag cgggaaaaat ggcagataat ccatccaata
      421 ccagatatgg cggccagcgg cagataccgg gttatcaact gctggaaaaa cttggttcgg
      481 gagcgatggc tgtggtttac aaggccagac agattagcct tgaccgcgtg gtagccataa
      541 aaattctctc aagccgtctt tccaaagatg aagaatacgt caaacgcttt tatgcggaag
      601 gccagatagc cgccaaactc aatcacccca atatcgtaca atccatcgac gtcggccaag
      661 ccggcgaata ttactatttc gtcatggaat acgtcgaagg caaaacggtt tacgaggaat
      721 tggctgcagg gaaaaaatac gctgaagccg aggcgctcga tatcgccata cagatggcca
      781 aagctcttca gcatgcccat gaacaggggc tgattcatcg cgacgtaaag ccgaaaaaca
      841 taatgattac gccaggcggc gtggcgaagc tcatggatat gggacttgcc cgcgtcgccg
      901 atgatgcgaa ggctatcgag tcggaggctg gccggctgtt cggtacgccg tattacatca
      961 gtcccgaaca gattctcggc cgcaaagaca tcgatttccg atgcgacata tattctctcg
     1021 gcgcgacgct ttatcacatg cttaccggcc ggccgccttt tcagggcaaa acctcaaaag
     1081 aagtcatgat aaagcacatc aaaaaggcgc cgcgttctcc ggacaggtat aatatggaac
     1141 tgtcgcgcgg catatgtgcg gtggtcgaaa agatgttggc taaaaatccg gctcaccggc
     1201 agtcgtcaac gaaggaactg gtcgaagagc ttcagtcggt cgaatttctg ctggatacga
     1261 gcgactcggc ggatacggct gccggaatcg aaaatattgc cgacaatatc cgtaaaatcg
     1321 cggaaatcgc cgataaaaaa tcgtccgttc ataagcctga ccctggtaaa aactccctga
     1381 aaacgccgct gaaaaacgaa cagccgtctg taatacgtgt agccgaacgg aatccgttaa
     1441 ataaacttct gctggccggt tttattgcaa gtctgatact caacctgatt ctcctgctgt
     1501 tatggctgaa gtgatgagcc atttccatac gcggtattta tggatgcagg gatatccgtt
     1561 ttttctttaa gtattgccgc cggaatgtcc gataattttc gtagaggctt gactaagcag
     1621 gcctgattct gggaattatc gcggtttgga tatggacatt cataatatag ccataaaaaa
     1681 accccaacgg acttacgtcc tgaggtatgc agagggttcg gaagagcagg ccgtcgagga
     1741 ggttatgcga tgggcggatg atccgtcgca tgaaatcgac tggcaggatg cagccgacat
     1801 cagttggcgc atacttaact ccgtcgcagc cgacatcaac cgcattgccg aagattcggc
     1861 acatcacgca ctaaaaggag gcagtcaggt ttgattatta cactgaccaa tgaattcctg
     1921 gattatatcc aggtcaagcg gaatttttct gcacatacaa ttcggtcgta tgccgcggat
     1981 ttgatgcagt tctgtcagta tctcgccgcc gccggcagcg gcgatccgct gcaactcggc
     2041 gaaactgcga cgtcgcttga taagcttccg ccgctggaag aggccgatgt cgccgctgtt
     2101 gaggctcgca tattatccgt cgaacctgtg gaaatccggg cgtacctggg cgtgttgagc
     2161 gaaaataaat attcacgttc gacgatagcc cgcaaacttg ccactttgcg aagtttttac
     2221 aaatatctcg ttcgtaccgg ccggctggag tcttcgccgg tcagcgtcat tcgtacgccg
     2281 cggcaggaca ggcggctgcc gaaatttctg gatatttcgc agatagaccg tctgcttgac
     2341 gcacccgaca aaaacactct gctcggcgct cgcgatagcg ccatattgga gacgatttat
     2401 tccgccggct tgcgaatcag cgagcttgtg gggctgaaca tcgaggatat ggatgagaac
     2461 gagggtgttt tgagaattcg cggcaagggc aggaaggagc gtttggcgct tctgggcagc
     2521 aaggctgtcg atgcgctgcg tcattacagg cgaaagcgcg atgcggcgtt ggggccgacg
     2581 aatcagggcg ccatgttcct gaaccgtgac ggcacgcgca tatccgaccg ttccatacgt
     2641 cgcaagctcg ataaatatat ggctatcgcc ggcttgccgt cgcacataag cccgcacacc
     2701 ctgcggcaca cgtttgcgac gcatatgctc aacgccggcg cagacctgag atgcgtccag
     2761 gagatgctcg gccatgaaaa tctttcgact acgcagatat atacccacct tacgacgcga
     2821 caactgcggc aggtctatga taaggctcac ccgcttatga aaagttgaaa catgctttat
     2881 gtgatgatga taatttggtg tttttgcatt tactttaccg gcagaggtct gtatcgcatg
     2941 tggtcgtccg taatcggcgg ggcggtgacg gactggctga tgctgcctgc gacgttgctg
     3001 tccgaactgc tttattccgg cggcaggctg atgacgggta ggccggtcta cgcgggcata
     3061 atagcgccgc agaatgtaac tgccgacccg tgcagggtgg ctatttcggg caaaaacgga
     3121 ttctgggtcg aaatgctctc agccggcctg gcgatg
//
LOCUS       DAONFI010000285         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38398_length_3155_cov_4.078387, whole genome shotgun sequence.
ACCESSION   DAONFI010000285 DAONFI010000000
VERSION     DAONFI010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38398_length_3155_cov_4.078387"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLK08_08655"
     CDS             <1..396
                     /locus_tag="PLK08_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPS53610.1"
                     /translation="KEVLTELATMLAEQLKYDAAAMVNAVFEREGLMSTGIGHGLAVP
                     HVRLEGLAEPALAVSVQPAGISDYESLDSMPVAVIVLIAAPAGQHEMYIRLLAQVADV
                     LKRDELREKVIAAATRGEADTVFKILTGK"
     gene            405..2102
                     /locus_tag="PLK08_08660"
     CDS             405..2102
                     /locus_tag="PLK08_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPS53611.1"
                     /translation="MLAEFDINHLPMLLVVGLSLFVGGLGARLFQKMRIPQVVGYITI
                     GLLIGRMGLKILNSEAIAQMRLFNYVALGVIGFMIGGELKKSVFQRYGRQFMAILFGE
                     GLTAFVFVSALVTLGGYLFTQNFATSMALGLILGAISSATAPAATVDVLWEYKTRGPL
                     TTSVFAIVALDDGLALVLYGLAASFAKHLIGTDVSGRGLLASVGTACYELVGAAILGG
                     IIGILLNFLLRFIKEHDKNLIFTLGALIVTLGLSMLMGVDVILSAMALGTTLTNLAPR
                     RSQETFRIIERFAPPIYVLFFVIVGARLTVGDMPAWMWVVAVLYVIGRTAGKMLGAFT
                     TARLTKSPQAVQKYLGLCLFSQAGVAIGLAIISSENFTGKYAYVGNAVIMIVTATTFL
                     VQIIGPPCVKLAVARAGEIGLNVTREDLIASYKVGDVMIRDPLVFRQATPFNVIMQTI
                     NETDYNTYPVVDDGNHLLGVITLNELKGGLGNIGLAQLLLAYDLMQPPSGIVQPNVNL
                     GEAMTKMREEDFEYLIVADYPDETGKCKLIGLVELRKVDRLLNRELIRRRDAADGQS"
     gene            2817..>3155
                     /locus_tag="PLK08_08665"
     CDS             2817..>3155
                     /locus_tag="PLK08_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPS53612.1"
                     /translation="MRVCFVGPTQSGKSSLFAAIVEAGGSNVDISRPDQPHVAMVKVP
                     DNRLVWLSEFYKPKKNTPAELELLDLPGFDLADEAGRTRSKTHWPAMRQSDMLVFVVR
                     SFRNDSVAAYR"
BASE COUNT          716 a          710 c          896 g          833 t
ORIGIN      
        1 aaagaggtgc tcacggaact ggccacgatg ctggctgagc agttgaaata tgacgccgcc
       61 gctatggtca atgccgtctt tgagcgtgag gggctgatga gtaccggcat aggccatgga
      121 ttagccgttc cgcatgtaag gcttgaaggc ttggctgagc cggcgcttgc cgtatctgtt
      181 cagccggctg gcatcagcga ctacgaaagc cttgattcca tgccggtggc agtgatagtg
      241 cttattgccg caccagccgg ccagcatgaa atgtatattc gcctgctggc acaggtggcc
      301 gatgtgctca aacgcgacga attgcgggaa aaggtcattg ccgcagctac gcgcggcgaa
      361 gccgacacgg tattcaaaat tcttacaggc aaatgagagg cgtcatgctc gccgaattcg
      421 acataaatca tcttcccatg cttctcgtag tggggctgtc gctgttcgtg ggtggcctgg
      481 gggcgcggct gtttcagaaa atgcgaattc cgcaggttgt gggatacatc acaatcggtt
      541 tgcttatcgg ccggatgggc ctgaaaatcc tcaacagtga agccatcgcc cagatgaggc
      601 tgttcaatta tgtggctttg ggcgttatag ggttcatgat tggcggcgaa ctgaagaaat
      661 ccgtttttca gcgatatggc cggcagttca tggcgatact tttcggcgaa ggccttacgg
      721 cgttcgtttt cgtaagtgct ctagttacgc tgggcggcta tctttttacc caaaactttg
      781 ccacgagcat ggctcttggc cttattcttg gcgcgatatc gtctgcgacg gctcctgcgg
      841 cgacggtcga cgttttgtgg gaatataaaa cccgcgggcc gcttacgacg tcggtttttg
      901 cgattgttgc gctggacgac ggtctggcgt tggtgctgta cggcttggcg gcgtcttttg
      961 ccaaacatct catcggtacg gacgtgtcag gcaggggatt gttggcgtcg gtggggacgg
     1021 cgtgttacga gcttgtgggg gcggcaattt tgggcggcat tatcggcata ctgctgaatt
     1081 tcctgctgcg gtttatcaag gaacacgaca aaaaccttat attcacgctt ggtgcgctta
     1141 tcgtaacgct ggggttgtcc atgttgatgg gagtggacgt gatactgtcg gcgatggcgt
     1201 tgggaacgac gctgacaaat ctggcgcctc gtcgcagcca ggagacgttc cggattatag
     1261 agcggttcgc accgccgatt tacgtgcttt tctttgtgat tgtgggcgcc aggctgaccg
     1321 tgggtgacat gccggcctgg atgtgggtgg tggcggtgct gtacgttatc ggccgtactg
     1381 ccggaaaaat gctgggtgcg tttaccactg ccagattgac caaatcgccg caggctgtgc
     1441 agaaatatct gggactctgc ctgttcagcc aggctggcgt agccatcggc cttgcaataa
     1501 tatcgtcgga aaattttacc ggcaaatatg cgtacgtcgg caatgcggtg attatgattg
     1561 tgaccgccac gacgtttctg gtgcagataa taggcccgcc atgcgtcaag ctggctgttg
     1621 ccagggcagg cgaaataggg ctcaatgtta cgcgggaaga tcttatcgcc agttataaag
     1681 tcggcgacgt catgatacgc gacccgctgg ttttcaggca ggctactccg tttaacgtca
     1741 taatgcaaac catcaatgaa acggattata atacctatcc ggtggtggac gacgggaatc
     1801 atctgcttgg ggtgattacg ctgaatgaac tcaagggtgg gctggggaat atcggcttgg
     1861 cacaactgct gctggcgtat gacctcatgc agccgccgtc gggcatcgtt cagccgaacg
     1921 tcaatctggg cgaagcaatg acgaaaatgc gggaagaaga ttttgagtat cttatcgttg
     1981 cggattatcc ggatgaaacc ggcaaatgta agcttatcgg gctggtggag ttaagaaagg
     2041 tagacaggct gctgaaccgt gaattgattc gtcggcgcga cgcggcggac ggccagagtt
     2101 aaaaaacggt tttttttaaa aaaagcttat tgagtgaaac gggttttttt atagcttttt
     2161 attctgctgt tgtctggtaa tggggctaaa aaactcattg agtgggatga atttttgaat
     2221 cctttttatt ctactcctgc ttggcggcga taaggttaaa aaaatgtcca ttaaaaaaga
     2281 ccccgttgga cgagagggct tttttcgcca tcttcgttct actgccgcct ttaatatcgc
     2341 tgggtgcgga gtagcagggt ctttttcgcc gaacagggct ggccttgtgg gcctctttcc
     2401 tgttcattaa attataggcg cctgataatg caataccaat taacagttga aattatttaa
     2461 tgcaaccgta tatagacgga aattgcaagg ttcaagaaac aagcgataag caaaacctcc
     2521 aacttttcag atatcgtata acgattgatg cggttcttag gcggaaattt tttgagaacc
     2581 gctttaaacc aactgtccca taatgccgta gcaataaaag aagcgaatct tgttgtctgc
     2641 tgcgagtttt tttatatgat gacatatctt gaaatgcagt aaaatctgta gagcgtattg
     2701 gtaaatcgtt atttgagtta cccctggcat tcggtcaata gctggctgca tcgcattttt
     2761 ggtaacgctc ttattatatt actataaatt gtttaaaaat tgatgaggta tcctgtatga
     2821 gagtttgttt tgtaggcccg actcagtcgg gaaagagttc gttgtttgcc gccatagttg
     2881 aagccggcgg ctctaacgtg gatatttctc ggcctgacca gccgcacgtt gccatggtca
     2941 aagtgcccga caaccggctg gtatggctga gcgaatttta caagcctaaa aaaaacacgc
     3001 ctgccgaact ggaactgctc gatttaccag gattcgacct tgccgatgaa gccggcagaa
     3061 cccgcagcaa gacacattgg ccggctatgc gccagagcga catgctcgta ttcgtcgtcc
     3121 gctcattcag gaacgattcc gtcgccgcgt accgc
//
LOCUS       DAONFI010000286         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38399_length_3155_cov_3.529677, whole genome shotgun sequence.
ACCESSION   DAONFI010000286 DAONFI010000000
VERSION     DAONFI010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38399_length_3155_cov_3.529677"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLK08_08670"
     CDS             complement(<1..316)
                     /locus_tag="PLK08_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53613.1"
                     /translation="MVATNDEKPSENALGIIDAMTPIGRKFEVGENILEYFGMDEQGN
                     IRYSENNGPLVVMSVDSWNANKAQHVEGYTDQVNPEVTDDNIADVSEPAEPEAIATEQ
                     PEE"
     gene            complement(405..668)
                     /locus_tag="PLK08_08675"
     CDS             complement(405..668)
                     /locus_tag="PLK08_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53614.1"
                     /translation="MKRNDWWIITKVRVNHAVTTGNFLSRDDARTYARHLSGYSTVEV
                     LNTAAFRRKYPRMAAERGLFAEQPQSIQKKTKAAPTLFPRACC"
     gene            complement(658..891)
                     /locus_tag="PLK08_08680"
     CDS             complement(658..891)
                     /locus_tag="PLK08_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53615.1"
                     /translation="MVAIIPAAFAPKDAAAYLGITLSKFYAEVMPSLEEMQLKPVFYG
                     YSQRAMWPRDVLDEYVRRCPSRPRTKKGVENEA"
     gene            complement(885..1079)
                     /locus_tag="PLK08_08685"
     CDS             complement(885..1079)
                     /locus_tag="PLK08_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53616.1"
                     /translation="MTVEGYKCQSCKYEFTDENAGVIMTSRASWDSAGGCPAEYEACC
                     PNCGKIVDDGDAADIEVEEW"
     gene            complement(1079..1405)
                     /locus_tag="PLK08_08690"
     CDS             complement(1079..1405)
                     /locus_tag="PLK08_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53617.1"
                     /translation="MNKVTYTPGPWDYEPYGDWYSFAGKDGRQDYTFRVEFDGDVSEK
                     EQLADVHIIAAAPELLEEYQNLLGRIEAMKSAMIRGGLRNWVKDWLSDDILDGNAITK
                     ATGEKD"
     gene            complement(1409..1690)
                     /locus_tag="PLK08_08695"
     CDS             complement(1409..1690)
                     /locus_tag="PLK08_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53618.1"
                     /translation="MIFTVTETPWTSCELADIHGTSAGRAKEQQTKIDDWILKIIARE
                     YVAAGTRSDQAANSIRCRIARLNKLFTKSPQRAMQHVTRLITIAKESRR"
     gene            complement(2026..2097)
                     /locus_tag="PLK08_08700"
     tRNA            complement(2026..2097)
                     /locus_tag="PLK08_08700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2063..2065),aa:Met,seq:cat)
     gene            2359..2760
                     /locus_tag="PLK08_08705"
     CDS             2359..2760
                     /locus_tag="PLK08_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPS53619.1"
                     /translation="MFDRKALRKFRAERNLSQAQLAKMVGCSSNMIAMYERGENNPRE
                     RQILRIAKALDVPVTALQADNESRDTVLDKVIMSLERMTPAERAEMLTHALRIEERRQ
                     KEKPVEYNTDNIIDPNFEKHMGTHKKKNQVG"
     gene            2772..>3155
                     /locus_tag="PLK08_08710"
     CDS             2772..>3155
                     /locus_tag="PLK08_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPS53620.1"
                     /translation="MPQIKIDKEYRKNLRDTKNMIAMAEKQDCNEAETRRRIERMFET
                     LMGYDPFRHLSRERAVRGAGETEHVDFSIELGEGEDKKPVIMIEIKRVCIDLAPKHLK
                     QVTSYAIDTGCEWILLTNSKQWNLYH"
BASE COUNT          787 a          780 c          758 g          830 t
ORIGIN      
        1 gctcttcggg ttgctctgtg gcgatcgctt ccggctcggc cggctcggag acgtcggcaa
       61 tattgtcgtc tgtgacttcc ggattcactt ggtcggtgta gccttctaca tgctgggctt
      121 tattggcgtt ccaactatca actgacatga cgaccagcgg gccgttattc tccgaatacc
      181 gtatattgcc ctgttcatcc atcccgaagt attcgaggat attttccccg acttcgaact
      241 tcctgccgat tggcgtcatc gcgtcaatta ttcccagtgc gttttcgctg ggtttttcat
      301 cgttagttgc taccattttt tgttactcca ttacgtgatt taaggtcatg ccggactctt
      361 ttgtgcgtta tcccaaccca ctggtcgccg gcgttattct gttatcagca gcaggcccgc
      421 ggaaacagcg tcggggccgc tttggttttt ttctgtatcg attgtggctg ttccgcgaac
      481 aagccgcgtt ccgctgccat tcgtgggtac ttccgcctga aggcagcggt attgagcacc
      541 tcaaccgtgg aataaccgga taaatgcctg gcgtaagtcc gggcgtcgtc gcggctcaaa
      601 aaattacccg tcgtcaccgc gtgattcacc ctcactttgg taataatcca ccagtcgtta
      661 cgcttcattt tcgactcctt tcttcgtcct cggccggctg ggacaacggc ggacgtattc
      721 gtccaaaacg tcacgcggcc acatcgcccg ctgcgaatat ccgtaaaaaa ccggtttgag
      781 ctgcatttct tcaaggctgg gcatcacttc cgcataaaac tttgacaggg ttatgcccag
      841 gtacgccgcc gcgtctttcg gggcaaacgc cgccggaata atcgctacca ttcttccacc
      901 tcaatatctg cggcatcgcc gtcatcaacg attttgccgc aattcggaca gcaggcctcg
      961 tattccgcgg gacagccgcc cgccgaatcc caactggcac ggctggtcat aatcacgccg
     1021 gcgttctcgt cagtgaattc gtatttgcag gattgacact tgtaaccttc gacggtcatt
     1081 agtctttttc tcccgtggct tttgtgattg cattgccatc aagaatatca tccgacagcc
     1141 aatccttaac ccaattacgc aaacctccgc gaatcatggc ggatttcatt gcctctattc
     1201 gtcccagcag attttgatat tcttccagca attcaggggc ggcggcaatg atatggacat
     1261 cggcaagttg ctctttttct gacacatcgc cgtcaaactc cactctaaat gtataatctt
     1321 gcctgccgtc ttttccggca aaactatacc aatcgccata tggttcatag tcccacggcc
     1381 ctggtgtgta agttacttta ttcatggttt acctcctgct ctcttttgcg atggttatca
     1441 gccgcgtaac gtgctgcatg gcacgctgag gggatttggt aaaaagttta ttgagccgtg
     1501 cgatacggca tctgatggag tttgccgcct ggtctgaccg agttccggcc gcaacgtatt
     1561 cgcgagctat gattttcagt atccagtcgt ctatcttggt ctgctgctct ttcgcccgcc
     1621 ctgccgatgt gccgtgtatg tccgccagtt cgcagctcgt ccacggggtt tctgtgaccg
     1681 taaatatcat cgttactgcc cgtcggttaa aatgcctttt caccccatac gtcctgtgga
     1741 gtgaaaagat ttttaaaaca gccgtaacgt ccgtgttagg tactcgtggg gtgaaaagtt
     1801 ttgactgcac aaaaaacgcc gtgcagctat gattgttaaa aaatgtggca ggccccgacg
     1861 cagggggaaa cgtcgaggca ctgccggccg gccggaggaa accggcatta acactgctgc
     1921 ggaaggaggt ggccgcaaca gactgaaatg gtctaaaggt cggacgggcg aaaaatccgg
     1981 attcgcccgt ccgcgagcct gtctggtgga gggagaccag acaattagcg ggagcgggaa
     2041 tcggacccgc agtcttcagc gcatgaggct gacgtgttgc cgttacacta tcccgcgata
     2101 taacggggag tgtgaacccc ccgccaagga gtccgattac gcaacaacca cgaagagtat
     2161 tcggacattt attggcttga tttgtgtttc gagttgtggt tgttgcgttg ttcatatgag
     2221 taataataca agtttctata aattaagtca acaaatattt acaagtaata ataaacaata
     2281 ttttatagat acttgtaaat attgcaaatt tctatattta tgatttttta aaaaagttgg
     2341 tttgatatga tatttttaat gtttgataga aaagcattgc ggaaatttcg ggcagaacgc
     2401 aatttaagcc aggctcagct tgccaaaatg gtaggctgtt caagcaatat gattgcaatg
     2461 tatgaacgcg gggagaataa tccgcgagaa cgtcaaatat tgagaattgc aaaggcttta
     2521 gatgtaccgg taactgcctt acaggccgat aacgaatccc gcgacacagt actggacaag
     2581 gttatcatgt ctctggagcg tatgaccccc gcagaacgtg cagaaatgct tactcacgca
     2641 ttgcgtatcg aggagcgtcg ccagaaagag aagcctgttg aatacaacac cgacaatata
     2701 attgacccca atttcgaaaa acatatggga actcacaaga agaaaaatca agtgggatag
     2761 aaaggaaaaa aatgcctcaa attaaaatcg ataaagaata caggaagaat ttgcgtgaca
     2821 caaaaaatat gatcgccatg gcagaaaaac aggactgcaa tgaggccgaa acccgtcggc
     2881 gaatagaaag aatgtttgaa accctcatgg gctacgaccc attccgccat ctttctcgcg
     2941 agcgcgccgt tcgtggggca ggtgaaaccg aacacgtcga tttttccata gaactcggcg
     3001 aaggtgaaga caagaaaccg gtcataatga tagaaatcaa aagggtctgt attgatttag
     3061 caccgaaaca ccttaaacag gtaacctcat atgccatcga tacaggttgt gaatggattc
     3121 tgctaacgaa ttctaaacaa tggaatcttt atcat
//
LOCUS       DAONFI010000287         3139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_38708_length_3139_cov_6.527562, whole genome shotgun sequence.
ACCESSION   DAONFI010000287 DAONFI010000000
VERSION     DAONFI010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38708_length_3139_cov_6.527562"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1223)
                     /gene="dnaN"
                     /locus_tag="PLK08_08715"
     CDS             complement(126..1223)
                     /gene="dnaN"
                     /locus_tag="PLK08_08715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPS53621.1"
                     /translation="MKVIAQTQALQDALNVLGSIVSTRTPKPVLECIKLVATANGLEL
                     MGTDLEVGCRYLVTAVQVEQAGEALVPATRFSGIVREATGDDSLTIESDKEATIVKGT
                     GSRFKIFGFDPAEYPAIDQFPAAGDFKVPAAVLKDMITKTLFATAKAHSHYAISGVLW
                     EATGKNLKLVATDGHRLAQAMGMLESASSSSATAIVPAKFMGLILRVCNPEDMLDVKI
                     EDNQILVKSSNATLMSTLVQGNFPKYSDVIPQNCDRHADINTGEFQHRVRQAALLTND
                     ESRGVKLSFQADSLIMSSRAPEAGEAEVQCPIVLNGNPMDIGFNPQFLLDALKVIDTD
                     MLRFEMSAPNKPAMIKAGPDFLYVLMPVDLG"
     gene            complement(1730..3091)
                     /locus_tag="PLK08_08720"
     CDS             complement(1730..3091)
                     /locus_tag="PLK08_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53622.1"
                     /translation="MSVKGKGQAFFKRGDEVADTGNWDFAIEMYVEGIQREPANIENG
                     YNKLREVALKRKAQGGKPPGILEQIKRRSGKDLEKNLANACYLWAKDPGSMLSMEQVI
                     IAAQKLELNEVIVWMAKLMFEAAKTTQKPQKRSFIFLTDIFEQTNELKLAVEACRDAV
                     NLSPQNSDPQLENRLNTLLANYTIQQGQYGQEGEGLRGVRDMEKQKELMQQDSLVKSE
                     DYLEQQIRKTKREYEESPDVPGKISGYVDALLKIETPENQQTAIDVLQQAYKATNTYN
                     YKMRIGDIKMRQHVIAVRQAKEAGNQEAYKKAYIDQLKFEITEFDERARNYPTDLGYK
                     FELGRRLFTIGKYDEAIAALQGAQNDPRRKLLVANYLGQSFMKKQWWQEAVDTFEKAL
                     SADIPEERTKDIRYYLANCYENLGDLKNAQESYSAIAQIDFNFKDVRSRLQTIRDKLK
                     QQV"
BASE COUNT          651 a          746 c          803 g          939 t
ORIGIN      
        1 gagaaattca ctgaatatct cgcgaaatgg tatgacataa atatcggcgt gaaaaggaaa
       61 tgattgaagc gactctcaaa attttttctt tttgagagcc gctccatatt tttcttaaaa
      121 aaatatcagc cgaggtcgac cggcataagt acatagagga aatcggggcc ggcctttatc
      181 atcgccggct tgtttggggc gctcatttca aaccgcagca tgtctgtatc gattaccttg
      241 agtgcgtcga gcagaaattg cggattgaaa ccgatgtcca tgggattgcc gttgaggacg
      301 attggacatt ggacttcggc ttcgccggct tctggggctc ggctggacat tataagcgag
      361 tccgcctgga agctcagttt cacgccgcga gattcgtcgt tcgtaagcag ggccgcctgc
      421 cgcacgcgat gctggaattc gccggtgttg atatcggcgt ggcggtcgca gttttgcggg
      481 atgacgtcgg aatatttggg gaaattgccc tgtacgagcg tactcatcag cgtggcgttg
      541 gatgatttta cgagaatctg gttgtcttca atcttgacgt cgagcatatc ttccggattg
      601 cagactcgca gaataaggcc catgaacttc gccggcacta tcgccgtcgc cgaagatgag
      661 cttgccgact cgagcatgcc cattgcctgt gccagccgat ggccgtccgt ggcaacgagc
      721 ttcagattct tgcccgtcgc ttcccataaa acgcctgata tggcgtaatg actgtgagct
      781 tttgcagtgg cgaagagcgt tttggttatc atatccttca aaacggcggc tggaactttg
      841 aaatcgccgg cggctgggaa ctgatcgatg gctggatatt ccgcagggtc gaacccgaaa
      901 atcttgaaac ggctgcctgt gcccttgacg atggtggctt ccttatcaga ttcgatagtc
      961 aggctgtcgt cgccggtggc ttcgcggacg atgccgctga atcgggttgc cggcacgagt
     1021 gcttcgccgg cttgctcgac ctgtactgca gttaccagat agcggcagcc cacttccagg
     1081 tctgtgccca tgagctccag gccgtttgcc gtcgcgacca gtttgatgca ctcaagcacc
     1141 ggcttgggcg tgcgggttga tactatactg cccagtacgt tcagtgcatc ctgcaatgcc
     1201 tgcgtttgtg ctataacttt cattttcccg ccatccttcc ttgtatacaa gataatgtat
     1261 tatagctgta aattcatacc ataaggtgta cggagctttt atacgatttt ttcagccgtc
     1321 ttgcaagaaa aaatgacggt ttatggggat attctgcaat ttcattttag agcaatatag
     1381 agttgtttgt catttttcgt tgcgacatta ttcaatagcg atttttacga aaaaataagc
     1441 ccgaataccg ctagagcata ttaaacttga ctttgcgctt gttgctgttt gcggcgtcgg
     1501 ctggctacgt caggctgcat cggaaaccat ataaggttgc ctgcttcgcc ttccttgcca
     1561 gcctcgtcct caacgacgca acaaggcgca atttcaaatt taacatgctc taaagcattc
     1621 gggcttattt gtctttgtac gtagaaatct attccaaaaa tcttggttgc ctgcaaaatg
     1681 gctcaatttt gacggcatcc atgttttggg atgggttcta ataaaaatat cagacctgtt
     1741 gcttgagttt gtcgcgtatt gtctgaaggc ggctgcgaac gtccttgaag ttaaaatcta
     1801 tctgggcgat ggcagagtag ctttcctggg catttttgag gtcgcccaga ttttcatagc
     1861 agttagccag ataataacgg atatcctttg tgcgttcttc agggatgtcg gcagagaggg
     1921 ctttttcgaa tgtatcgaca gcttcctgcc accactgttt tttcataaac gactggccga
     1981 gataatttgc caccagcaac ttacggcgtg gatcattttg tgcaccctga agtgcagcga
     2041 tagcttcgtc gtatttgccg attgtgaata accgtcggcc cagctcgaat ttatagccca
     2101 ggtcggtcgg atagtttctg gctcgttcgt cgaattccgt gatttcgaac ttgagctggt
     2161 cgatatatgc ctttttgtaa gcttcctggt tgccggcttc cttcgcctgc cgtacggcga
     2221 tgacgtgttg acgcatcttt atatcgccga ttcgcatttt gtaattgtac gtgttggttg
     2281 ctttgtaggc ttgctggagc acgtctatag ccgtctgctg attttccggc gtttctattt
     2341 tcagcagggc gtctacgtaa ccggatattt tacccggcac gtcaggcgat tcttcatatt
     2401 cgcgtttcgt ctttcgtatt tgctgctcga gatagtcttc gctttttacg agcgaatcct
     2461 gctgcatgag ttctttctgt ttttccatat cgcgtacgcc acgaaggcct tcgccttcct
     2521 ggccgtactg gccctgttgt atggtgtaat tagccaaaag cgtgttaaga cgattttcaa
     2581 gttgcgggtc gctgttttgc ggcgaaaggt ttacagcgtc acggcatgct tcgacagcca
     2641 gtttgagttc attagtctgt tcgaagatat ccgtaagaaa gatgaaggag cgtttttgag
     2701 gtttttgggt ggttttggcg gcttcgaaca tcagcttagc catccatacg atgacttcgt
     2761 tgagttccag tttttgcgcc gcaatgatta cctgttccat ggataacatc gagcccgggt
     2821 ctttagccca gaggtaacag gcgtttgcga ggtttttttc cagatccttg ccggagcggc
     2881 gtttaatctg ttccaatatg cctggcggct tgccgccttg cgctttgcgt ttgagggcca
     2941 cttcgcggag tttgttgtag ccgttttcta tgtttgccgg ctcacgctga atgccttcaa
     3001 catacatctc gatggcgaaa tcccagttgc cggtatcagc cacttcatcg ccgcgtttga
     3061 aaaaagcctg tcccttgcct tttacgctca tcgcatgcct cttacagctt gagtaacatt
     3121 atttaagctg gccgatttt
//
LOCUS       DAONFI010000288         3108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_39298_length_3108_cov_3.426138, whole genome shotgun sequence.
ACCESSION   DAONFI010000288 DAONFI010000000
VERSION     DAONFI010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39298_length_3108_cov_3.426138"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..2638)
                     /locus_tag="PLK08_08725"
     CDS             complement(191..2638)
                     /locus_tag="PLK08_08725"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPS53623.1"
                     /translation="MSSIPKIWWVAPISALLALAAAAFFFHQVKKAHAGDANMIEIAG
                     HVTEGAMAYLKRQYKVVGIFFIVVSAILYVMGQIGVQHKIVFVAFLTGGFFSGLCGWI
                     GMKTATMASSRTAQGAKEGLNRGLQVAFRAGAVMGLVVVGFALIDISGWFVALRAYGM
                     NLVDITVVMLTFGMGASSQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAT
                     IADNVGDNVGDVAGMGADLYESYCGSILATAALGVSGAVALGMDSSTQLKMLATPMVI
                     AGIGILLSIVGVFMVRCKEGASMRQLMGALNIGVFGSSLLIAVASYFVCDYMLGGIAG
                     VSSTGIWGAIIVGMISGIIIGKATELYTSYDFQPTKNVAENAQTGPATVIISGLAEGM
                     KSTWMSLLTIIVAIILAYIMAGGRDEMLMGLYGVGIAAVGMLATLGITLATDAYGPIA
                     DNAGGNAEMTHQDPEVRRRTDALDSLGNTTAAVGKGFAIGSAALTALALLAAYVEEVR
                     VGQIREVENSPAVVKTVEVDRFVHIGNGKFAIAVKDKNGVERPRAFMALTGRYDDIVD
                     KKFTMKEITEPNGEDVRTLTLTAKDEKGDVFTRQISVVSSAKASLRQFMDYYGVNLMN
                     PKVLCGLFAGVLLVFLFSAMTMKAVGRAAMAMVMEVRRQFKEIPGILEGTAKPDYASC
                     VDISTRGAQKEMLAPALLALAVPVVVGLVFDVAGVLGLLAGGLACGFAMAIFMANSGG
                     SWDNAKKFIETGQYGGKGSQAHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVSVVFA
                     GLIVKFAPVISQWLHLD"
BASE COUNT          766 a          931 c          746 g          665 t
ORIGIN      
        1 cagcggcatt gtatcgtcaa aacggctatc ctgcaatcgc gaatttatgg cacattaact
       61 tggccctgtt ctaaaagcct tttaagccgc cggcagctat cggtttttcc caaacgcaaa
      121 ataaaactgc ggacagccgg cgtttaattt catccggtcg tccgcagttt tcatgcgtta
      181 tttaaggcca tcagtccaga tgcagccact ggctgattac aggggcaaac ttgacaataa
      241 ggcctgcaaa gacaactgaa accatgctca tcagcttgat gaggatgtta aggctgggcc
      301 ctgacgtatc tttgaagggg tcgccgacgg tatcgccgac gacggcggct ttgtgagcct
      361 gactgccctt gccgccatat tggccggttt cgatgaattt tttggcgtta tcccatgagc
      421 cgcctgagtt agccatgaaa atcgccatgg caaaaccaca cgcaaggccg ccggcaagca
      481 ggcccaacac gccggctaca tcaaatacaa ggcccacaac aaccggcact gccaacgcca
      541 ataacgccgg agcgagcatc tctttctgcg cgcccctggt ggatatgtcc acacagctgg
      601 cgtaatcagg tttggctgtg ccttcaagaa tgccggggat ttccttgaac tggcgacgaa
      661 cttccataac catggccatc gcggctctgc cgacggcttt catcgtcatg gcgctgaaca
      721 ggaacaccag caatacgccg gcaaacaggc cgcagagtac cttcggattc atgaggttaa
      781 cgccgtagta gtccatgaac tgccgcagcg acgccttcgc gcttgaaacc acgctaatct
      841 ggcgtgtaaa tacatcgccc ttttcatctt ttgccgtcaa tgtcagcgtg cgaacatctt
      901 cgccgttcgg ctcggtgatt tccttcatgg tgaatttttt gtcgactatg tcgtcatacc
      961 ttccggttaa cgccatgaac gctctggggc gttccacgcc attcttgtcc ttgacggcaa
     1021 tggcgaattt accgttgccg atgtgcacaa acctgtctac ttcgacagtc ttgacgacgg
     1081 cgggtgaatt ttccacttca cgtatctgac cgacgcggac ttcttccaca tacgccgcca
     1141 gtagtgccag cgctgtaagc gcggctgaac cgattgcaaa tcccttgccg acggctgccg
     1201 tagtattgcc cagcgaatcg agcgcatcgg tacggcgacg gacttcagga tcctggtgcg
     1261 tcatttccgc gttaccgccg gcgttgtcgg cgataggccc gtaagcgtcg gtagccagcg
     1321 ttatgcccag cgtagccagc atgccgacgg ctgcaatgcc cacgccgtaa aggcccataa
     1381 gcatctcatc gcgtccgcca gccatgatat acgccaatat gatggcaacg atgatggtca
     1441 gcagactcat ccacgtggac ttcatgccct cggcgaggcc ggagattata accgtcgccg
     1501 ggccggtctg ggcgttttca gcaacgttct tcgtcggctg gaaatcataa ctcgtataga
     1561 gttcggtggc tttgccgatg atgatgccgg aaatcatgcc cactataatt gcgccccata
     1621 ttcccgtgct gcttacgccg gcgattccgc caagcatgta atcgcacacg aaatacgacg
     1681 ccacggcaat aagaaggctc gaaccgaaaa cgccgatgtt caaagctccc atgagctgcc
     1741 gcatcgacgc gccttccttg cagcgaacca tgaacacgcc gacgattgac agcaatatgc
     1801 cgatgccggc gatgaccatc ggcgtggcga gcattttcag ctgcgtcgat gaatccatgc
     1861 caagagccac cgcaccggat actcccaacg ctgcagtagc cagaatcgaa ccgcaatacg
     1921 attcgtagag gtcggcaccc atgccggcga cgtcgccgac gttatcgccg acgttatcag
     1981 cgatggtagc cggattgcgg gggtcgtctt cgggaatgcc ggcttcaacc ttgccgacaa
     2041 ggtctgcacc tacgtcggcg gctttcgtgt atatgccgcc gccaacacgg gcaaacagcg
     2101 cctggctgga cgcacccatg ccgaatgtca gcattaccac agttatatcg accaggttca
     2161 tgccgtaagc acgcagggca acgaaccagc cggaaatgtc aatcaacgca aaccccacga
     2221 ctaccaggcc cataaccgcg ccggcacgga acgcaacctg caagccgcgg ttaagacctt
     2281 ccttagcacc ctgagccgta cggctggagg ccatcgttgc ggtcttcatg cctatccagc
     2341 cgcaaagtcc gctgaaaaaa ccgccagtca aaaatgccac aaaaactatt ttatgttgaa
     2401 cgccaatctg acccatgacg taaaggatgg ctgataccac gataaaaaat atgcccacaa
     2461 ccttgtattg acgcttcaga tacgccatcg caccttcagt aacatggccg gcgatttcaa
     2521 tcatgttcgc atcgccagcg tgcgcttttt tcacctgatg gaagaaaaac gccgccgccg
     2581 caagtgccag caacgcagat atcggcgcca cccaccatat tttaggaatc gacgacaggg
     2641 cgcatgcacc cttcaccagc gaagatgcgc tttccggcac aacaacggtc tgctccgtca
     2701 cactcacctg agccgtctgg gcaaaagccg atgacgaaaa aatcgccata cacgccgtag
     2761 ccgtcatcac ggctgccaga acttttccgt ttacgattct cagcagggaa ttcacttttt
     2821 cactactaac aaaagtgcag ctaaaacaag ggacgcaacc tttgcacccc tgttgcattt
     2881 tctaatatga ccttttagag catattaaac tcactctagc actctgtaac agagcacatt
     2941 aaattcactt tcatcatccg cccgaaataa ccacgcgcac acaaaaacca caagactgaa
     3001 tagaatccag aaaacatctt atgtgaaaca gccgaataac acctttcggc ctatctgatt
     3061 tatcatatgc atccaaccga cgttaaagcc ggctgacgcc tgaaaagc
//
LOCUS       DAONFI010000289         3100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_39416_length_3100_cov_5.337931, whole genome shotgun sequence.
ACCESSION   DAONFI010000289 DAONFI010000000
VERSION     DAONFI010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39416_length_3100_cov_5.337931"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLK08_08730"
     CDS             complement(<1..374)
                     /locus_tag="PLK08_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPS53624.1"
                     /translation="MSKVIENIIAKARSAGKTIVLPEADDQRVLLAAQKILAEGYAKI
                     ILLGDPAKIQADAKACSADIAGAQIVNPLTDSARDSYIDTLWEKRKAKGMTREDAEKM
                     MNQVVFYGGAMLAAGRADGVVVG"
     gene            407..1546
                     /locus_tag="PLK08_08735"
     CDS             407..1546
                     /locus_tag="PLK08_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53625.1"
                     /translation="MGKNRLPAIGMCVASRFSGCRNKANRIQLFYLDVESQMTRNKYL
                     LAEKIVVYGAAAAMAAVIYFAFAPLILPVDPTGPIAPLLTGEFGYEILLVSVLLAAGL
                     AFAAGGVLVRPGSFSLVLLAGMAGVSMKSGMFRVVLWQKGTDLGIWVCMLAELLILAA
                     AMLIVDFAASLFRRRAIRRGGFTALSARLSDEQLASLAEDKLILPERQMLWSRDVLPN
                     MAVENHFLSLLLLLCGLNRFKSSHRTLAVMALKNAAGFTVVGMAVGLVAAYVLAGSVA
                     RGQLVFAAFGAFWLAAEAGYYLYPVPDSFPAWLLPVMVGAAYYVIAMFTSSDLNPDQW
                     TKLKNVFQILPVDWIFAGGAGAMFSLWSSDRSKEVRIIEKLDIQE"
     gene            complement(1564..1998)
                     /gene="ybeY"
                     /locus_tag="PLK08_08740"
     CDS             complement(1564..1998)
                     /gene="ybeY"
                     /locus_tag="PLK08_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPS53626.1"
                     /translation="MSKIIINIDRRNGGVKLTKKTIADLVSFVAASEHKRVAELDLAI
                     VSSAEMAEMNHRFLGHEGDTDVITFDLTEDDENIVVQLIVCDEVAFRVAEENGLTPVQ
                     ELSLYIIHGLLHTMGYDDITPAKFRRMKKRQNELLNKFRNFA"
     gene            2022..>3100
                     /locus_tag="PLK08_08745"
     CDS             2022..>3100
                     /locus_tag="PLK08_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPS53627.1"
                     /translation="MKSAIRQWQSWAFFSKVSAMNNNEKHGGWNWPMSLDWPADPKKR
                     KQVKIIRIAAVTMFVVLLFIPTLQLELGTIKNQRKAAKYDRLMAAGKLTSQQIAAGRP
                     KSHKGAVNRWRVACEQFWQGKNIYLTPDQASKLPESSENDVTLHPNMPFIVILLSLFA
                     MPPMAVGGLIFTILKIIAILLGVLATAEIVSDVNKKVPDWIIGVALAFSVLFFIGDIQ
                     HANTNGFVFAFVAAHLWLYRRGRDYAAGAMLAVAICIKMTPALFILYWLYQRNWKLLG
                     GCIAAGILFAILVPAMFMGPAHYCELTHSWLDNLIFKGVGGAWYPVHINQSLPAVLGR
                     YLLGDQQGANYLWNADDNPNGISEFA"
BASE COUNT          673 a          722 c          882 g          823 t
ORIGIN      
        1 cccacgacta cgccgtcggc acggccggcg gctaacatgg ctccgccgta gaaaacaacc
       61 tgattcatca ttttttccgc atcttcacgc gtcatgcctt tggctttacg cttttcccaa
      121 agagtgtcga tgtacgaatc gcgggctgaa tcggtaagcg ggttgacgat ttgtgcgccg
      181 gcaatgtcgg cggagcaggc ttttgcgtca gcctgtatct ttgcagggtc gcccagaagg
      241 ataatcttcg cataaccttc agccaggatt ttctgtgcag ccagcagtac acgttggtcg
      301 tccgcttccg gcagcactat ggttttgccg gcggagcggg cttttgcgat aatgttttca
      361 ataactttac tcattttttt tgcctttgcc tttgaccttg agtaacatgg gtaaaaacag
      421 actgcccgca atcgggatgt gtgtggcgtc ccgtttttcg ggatgcagga acaaggccaa
      481 caggatacag cttttttacc tcgatgtcga gagccagatg acaagaaata aatatctttt
      541 ggcggaaaaa attgtggttt acggcgcggc ggcagcgatg gcggcggtga tttatttcgc
      601 gtttgcgccg ctgattctgc ccgtcgaccc gacaggcccc atagccccgc tgcttactgg
      661 agagtttgga tacgaaatct tgctggtaag tgttttactg gcggcaggtt tagcctttgc
      721 cgccggcgga gtgcttgtgc ggccgggcag tttttcgctg gtattgctgg cgggcatggc
      781 gggagtgtcc atgaaaagcg gcatgtttcg tgtcgtgctt tggcaaaaag gaacagactt
      841 gggaatatgg gtctgcatgt tagccgaatt gctgatattg gcggcggcga tgctgattgt
      901 ggattttgcg gcaagtcttt ttcgcaggcg tgcaataaga cgcggcggat ttacggcgct
      961 ttcagccaga ttgagcgacg aacaactggc gtcgctggcg gaggataaat tgattctgcc
     1021 tgaacgtcag atgctctgga gtcgcgatgt gctgccgaat atggccgtgg aaaatcattt
     1081 tttatcattg ctgctgctgt tgtgcggttt gaacaggttt aaatccagtc acaggacgct
     1141 ggcggtcatg gcactcaaaa acgcggctgg tttcactgtt gtgggaatgg cggtgggatt
     1201 ggtggcggcg tatgtgttgg ctgggtctgt tgccagggga caactggttt tcgcggcatt
     1261 tggagcattc tggctggcgg cggaggcggg gtattacctg tatccggtgc cggattcgtt
     1321 tccggcgtgg ctgttgccgg ttatggtggg tgctgcgtat tacgttatcg ccatgttcac
     1381 gtcgtcggac ttaaatcccg accagtggac aaaactgaaa aacgttttcc agatattgcc
     1441 cgtggactgg atattcgccg gcggagcggg cgccatgttc agcctctgga gttccgaccg
     1501 ctcaaaagaa gtccgaatca tcgaaaaact cgacattcag gaatagtgac gcaaaaagcg
     1561 gaatcacgca aaattcctga atttgttgag cagttcgttc tgtcgttttt tcatgcgacg
     1621 aaattttgcg ggggtgatgt cgtcgtagcc catcgtgtgc agaaggccgt gaatgatgta
     1681 caacgaaagt tcctgtaccg gcgtcaagcc gttttcttcc gcgactcgaa aggctacctc
     1741 gtcacatact atcaactgta ccacgatgtt ttcatcgtct tcggtaaggt cgaaagttat
     1801 cacatccgtg tcgccctcat ggcccagaaa acggtggttc atctccgcca tttctgcgga
     1861 cgatactatc gcaaggtcga gttctgcgac gcgcttgtgc tcgctggcgg ctacgaatga
     1921 caccaggtcg gctatcgttt tttttgtcag cttcacgccg ccgttgcgtc ggtcgatatt
     1981 tatgatgatt ttcgacatgt gtctgaggat accagcgagg tgtgaaatct gcaatacgcc
     2041 agtggcaaag ttgggcgttt tttagtaaag tatcagccat gaataacaac gaaaaacacg
     2101 gcggttggaa ttggccgatg tctctcgact ggccggctga cccgaaaaaa cgcaaacagg
     2161 tcaaaataat ccgtattgcg gcggtgacga tgtttgttgt gctgcttttc atcccgacgt
     2221 tacagttgga gctcggcacg ataaagaatc agagaaaagc tgcgaaatat gacaggctga
     2281 tggctgccgg aaaacttact tcgcaacaga tagcggctgg ccggccaaaa tctcacaaag
     2341 gcgcagtcaa ccggtggcgc gttgcgtgtg aacagttctg gcagggcaaa aatatctatc
     2401 ttacgcctga ccaggcgtca aaacttccgg aaagttcaga aaatgatgta acactgcatc
     2461 cgaatatgcc tttcatcgtc attcttcttt cgctttttgc gatgccgccg atggctgtcg
     2521 gcgggctgat attcacgata ttgaaaataa tcgcaatatt gctcggtgtg ctggcgaccg
     2581 cggagatagt atccgacgtc aataaaaaag tcccggactg gataatcggc gttgcactcg
     2641 cgttctccgt gctctttttc ataggagaca ttcagcacgc caataccaat ggatttgttt
     2701 ttgcgtttgt cgcggcgcat ctgtggcttt atcggcgagg cagggattat gcagcgggcg
     2761 ccatgcttgc cgtggcgata tgcataaaaa tgacgccggc attgttcata ttatattggc
     2821 tgtatcagcg gaactggaaa ctgctcggcg gatgcattgc ggcaggaatt ttattcgcga
     2881 ttttagtgcc tgcgatgttc atggggcctg cacattattg cgaacttaca cattcctggc
     2941 tggataacct gatattcaaa ggtgtaggcg gcgcgtggta tccggtacat atcaaccagt
     3001 cgttgccggc tgtgctgggc agatatctgc tcggcgacca gcagggtgcg aattacctct
     3061 ggaatgccga tgacaatccg aacggcataa gtgaatttgc
//
LOCUS       DAONFI010000290         3100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_39422_length_3100_cov_3.534319, whole genome shotgun sequence.
ACCESSION   DAONFI010000290 DAONFI010000000
VERSION     DAONFI010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39422_length_3100_cov_3.534319"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLK08_08750"
     CDS             complement(<1..652)
                     /locus_tag="PLK08_08750"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPS53628.1"
                     /translation="MARYGRLKTLSLMKEIGMIPVFYSADFEIAKNVIAACADGGAKV
                     IEFTNRGDRAIEVFTQLEKYCAASRPDIVLGVGSIVDAPTAGLYISSGANFVVGPVLD
                     EAVAVLCNTRKIPYSPGCGSATEINKAHSLGVEICKVFPGSCVGGPDFVKSVRGPMPW
                     SEIMPTGGVSPTKESLTEWFKAGIVCAGMGSNLLTKELIAKNDYAGITRKVRETMEL"
     gene            complement(906..2024)
                     /gene="dprA"
                     /locus_tag="PLK08_08755"
     CDS             complement(906..2024)
                     /gene="dprA"
                     /locus_tag="PLK08_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPS53629.1"
                     /translation="MRDEKKIREYLRLTLADGVGPKTFKQLVEFFGSPLDVALAGSFS
                     WQRVEGVGPKTVAAMQSVTDEVVDDELAVASRHDTRIICLDDDDYPPLLKRIHNPPPV
                     LYVRGCMEKEDAVAISIVGSRRCTHYGLEQAERFGGLLGRAGFTVISGGARGIDTAAH
                     RGAFYSGGRTIIVQGTGLANPYPRENVPFFDTILAGGSGAMISELPMRTTPGPGNFPA
                     RNRIISGMSLGVIVVEAGLPSGALITAREALEQNREVFAVPGRVDSPQSTGTHQLLRD
                     GATLAADLDDILKNLGQVGAELDRREPLPSPVKPQNLTPTQDALYSLLAEKAMTLDEL
                     CRATSGQPGQIAGDLTLLALRGAITQQPGNVFTLKNRH"
     gene            2300..>3100
                     /locus_tag="PLK08_08760"
     CDS             2300..>3100
                     /locus_tag="PLK08_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPS53630.1"
                     /translation="MAETFHTLIAKNKRNSFILMTLFMIFFVGLGLLIGTVWGSGEDR
                     SGEYQVNWHFSIMIAVIAGGIAFLLTLLSFYGGHSVLMAVSGAREIRKSDDPQLFNVI
                     EELAIAAGQPMPKIYVMDTAAMNAFATGRDPQHASVAITAGLRQKLTRDELQGVMAHE
                     MSHVRHYDIRYATLMAVMVGCVVMLSDIFLRSMIFSGGRRSRRDSNSGGGAQAILMVI
                     ALILAILSPILAKIIQMALSRQREYLADAGAVELTRNPEGLCSALQKIS"
BASE COUNT          768 a          850 c          746 g          736 t
ORIGIN      
        1 tcaattccat cgtttcacgg acttttcgcg ttatgccggc ataatcattc ttcgcaatca
       61 attctttcgt aagaagattc gaacccatgc cggcacagac tatgcctgct ttaaaccatt
      121 cggtgaggct ttccttcgtc ggagaaactc cgccggtggg cattatttcg ctccacggca
      181 tggggcccct gacggacttg acgaaatccg ggccgccaac gcagcttccg ggaaacacct
      241 tgcatatttc aacgcccagc gaatgagcct tgttgatttc cgtagcactg ccgcagcctg
      301 gcgaatatgg aattttgcga gtgttgcaca gaacggcaac ggcttcgtcc aaaacagggc
      361 cgactacgaa attggcgccg gaggatatgt aaaggccagc cgtaggagca tccactatcg
      421 agccgacgcc aagaacaata tcgggccttg aggcggcaca atacttctca agctgggtaa
      481 aaacctctat cgccctgtcg ccacgattgg taaattctat cactttcgct ccgccgtcag
      541 cacaggcggc gattacgttt ttggcaattt caaaatccgc actgtaaaaa accggtatca
      601 tgccgatttc tttcatcaaa ctcagcgttt tgagtcttcc gtatctggcc atttttttcc
      661 ttctttcaaa gttcattcat ttacatggag tgtccggcag aatattgtgt gacagacggc
      721 aactattggc tacaaatctg caaaataacg gcagtaatat taaacgattt tccaaacatg
      781 ttcaagcaga agctgtttaa aaatcgtccg ttccgacaaa ccagcaatac tggcctgatt
      841 ttttaaaaaa attctgaaaa tttacgaaaa ttatttgtta tagatgtgtt tttcgaaacc
      901 cgtttttaat gccggttttt cagtgtaaac acatttccag gctgctgggt tatcgctcct
      961 cgcagagcca gcaacgtcaa atcgcccgca atctggccgg gttggccgct ggtggcacgg
     1021 cagagttcgt cgagagtcat ggctttttcg gcaagcaggc tgtaaagagc atcctgtgtg
     1081 ggggtgagat tctgcggctt gaccggcgaa ggcagcggtt cgcgtctgtc gagttctgcg
     1141 ccaacctggc cgaggttttt caaaatatca tccaaatccg cagccagcgt cgctccgtca
     1201 cgcagtaatt gatgcgttcc ggtggactgc ggcgaatcaa ccctgccggg cacggcaaaa
     1261 acctcacgat tctgttccaa cgcttccctg gcggttatga gcgcgccgct gggcaggccg
     1321 gcttcgacga ctataacgcc caggctcatg ccggaaatta tgcgattacg cgcaggaaag
     1381 tttcccggcc cgggcgtagt tcgcatcggc aactcggata tcatcgcccc gctgccacct
     1441 gccagtatcg tgtcgaaaaa cggcacgttt tcacgcggat agggattcgc caggcccgtg
     1501 ccctgtacga ttatcgtccg gccgccggaa taaaacgccc cgcgatgagc cgccgtgtca
     1561 atgcctcgcg cgccgccgga tatcaccgta aaaccggctc ggccaagcaa gccgccaaat
     1621 cgctccgcct gctccaaacc ataatgagta caccgccgcg aaccaactat ggaaatcgca
     1681 accgcatcct ccttttccat gcaacccctg acgtaaagaa ccggcggcgg attgtggatg
     1741 cgtttgagca gcggcggata atcgtcgtcg tcaaggcata ttatccttgt gtcatgacgg
     1801 ctggcaactg ccaactcgtc atctacgact tcatcggtca ccgactgcat ggcggctaca
     1861 gttttcggcc cgacgccctc tacccgctgc caactaaaag agccagctaa cgccacatcc
     1921 agcggcgaac caaaaaactc gacaagctgc ttgaatgttt tagggccaac gccgtcagcc
     1981 agcgtaagcc gaagatattc tcgtattttc ttctcatccc gcatgtcaaa gcattatccg
     2041 aatttacaaa aaatccgcaa tattttttaa aaaaggtctt atgcctatga agttcataaa
     2101 acatttttaa aatacattca cacttattaa cgcttatcag gcctgttaaa atatttttaa
     2161 aacattttta aaaataacgc atatttccaa aaattcgata aatcttatac ctgtaatttc
     2221 acgttaaagc ggggaaatgg acttcaattt tcgtacgatt atgatatact tccggactga
     2281 attccggagt ttttagaata tggcggaaac tttccataca ttgatagcca aaaacaagcg
     2341 aaacagcttc atattgatga cgctgttcat gattttcttc gtcggcctgg ggttgctgat
     2401 aggcactgtc tggggcagcg gcgaagaccg gtctggcgaa taccaggtga actggcactt
     2461 cagcattatg atagccgtaa tcgcaggcgg catagcattc ctgctgacgc tgctgagttt
     2521 ttacggcggc cacagcgtgc tgatggctgt cagcggcgca cgtgaaatac gaaaatccga
     2581 cgaccctcag cttttcaacg tgattgagga gctcgcaatc gccgccggcc agccaatgcc
     2641 caaaatatac gtcatggaca ctgcggccat gaacgcattc gccaccggcc gcgacccgca
     2701 gcatgcgtcg gttgcaataa cagccggcct acgccagaaa ctgacgcgag acgaactcca
     2761 gggcgtgatg gcccacgaga tgagccacgt cagacactat gacatccgct atgcgacgct
     2821 gatggctgtc atggtcggct gcgtcgtgat gctttccgac atctttttac gcagcatgat
     2881 attcagcggc ggccgaagga gtcgtcgcga cagcaacagc ggcggcggag cacaggcgat
     2941 actgatggtc atcgcactga tattggcaat actctcgccc atattagcca aaattatcca
     3001 gatggcgctg tcccgccagc gggaatacct cgccgatgcc ggtgccgttg aactcacccg
     3061 caaccccgaa ggcctgtgca gcgcgctgca gaaaatatcg
//
LOCUS       DAONFI010000291         3090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_39584_length_3090_cov_3.739044, whole genome shotgun sequence.
ACCESSION   DAONFI010000291 DAONFI010000000
VERSION     DAONFI010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39584_length_3090_cov_3.739044"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2967)
                     /locus_tag="PLK08_08765"
     CDS             complement(<1..2967)
                     /locus_tag="PLK08_08765"
                     /inference="COORDINATES: protein motif:HMM:NF000282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HPS53631.1"
                     /translation="MYSRFIIERPVLAAVISIVIIIAGLVTMSALPIDLYPDITPPTV
                     TVTTTYPGADAVTLNDTVAQVIEEQVNGVEGMIYMSSTCANDGTYTLTVTFEVGMDLD
                     MATVLTQNQVTIATPQLPDEVQRQGVSTQKKSTNFVLAVNLYSPNNTYNDLELSNYAS
                     INMVDTLARVPGVGNVTIFGSKDYGMRIWLNPDAMHARQLTVTDVTDAVRSQNVQVAA
                     GNLGQPPCPKGTEFQYTVTVTGRLSTPEEFKNIVVKVGPDGHPVKIKDIGTVELGSQL
                     YNSYSTYNGKPSATIAIYQLPGSNAIDVEKGCKAELERLASRFPKDLKYNVTYDSTWF
                     IHASINEITVTLFQAVGLVVLITFVFLQDWRTSLIPALTIPVSLIGTFIVMALLGFSI
                     NTLTMFGLVLAIGIVVDDSIVVVENTQRKIDELGLAPREAAIAAMNEVIGPCIATTLV
                     LLSVFVPTAFMGGISGRLYQQFSLTIAASTAFSTVNALTLSPALCGVLLRPTSKTKFF
                     FFDWFNKIFAATERFYGGIVKMLVRKLVIVAILFLGITVLTWLGFVTLPTGFIPTEDQ
                     GYFMAVIQLPDAASLERTSAVTTRATDMVMKTPGVANVISIGGMSMIDSTISSNTASL
                     IVMLDDWDKRKTPELQINAIVEKLADEFKTIEEARIMSFVPPTIPGLGTTGGLQMQLQ
                     NYGNMPLPDLQAASVRLMQMAAGNPEVKDVFTTFRANVPQIHLDIDRDKVMTQGIALG
                     DVFDTLQVYLGSLYVNDFNLYGKTFQVKMQALSQYRVNPEDVGLLDVRDKNGNMVPLN
                     ALLVAKPSFGPQTITRYNMYESATFLGDSGEGYSSGQAMDAMTDMVHELPGFGLEWTG
                     MSFQEQAAKGQAPFIFALGAVFVYLFLAAQYESFLLPISVILSVPFALLGAVAANWMR
                     EYDNNIYTQIGFVLLIGLCSKSSILIVEFAKAKHEEQGQDRFTAAIEAARLRFRAILM
                     TAFSDIFGWFPLVVATG"
BASE COUNT          741 a          836 c          811 g          702 t
ORIGIN      
        1 gccggtggca accacgagcg gaaaccagcc gaatatgtcg gaaaacgcag tcatgagtat
       61 cgcgcggaat cgcagacgcg cggcttcgat ggcggcagtg aaccggtcct ggccctgctc
      121 ttcgtgtttg gctttggcga attcaacgat aagaatcgaa ctcttgctgc aaaggcctat
      181 cagcagaaca aagccaatct gcgtataaat attgttgtca tactccctca tccagttagc
      241 cgccaccgca ccgagcaacg cgaacggcac agacagaatg accgatatcg gcagtaaaaa
      301 actttcatat tgtgccgcca gaaacagata gacgaatacc gcgcccaggg caaatatgaa
      361 cggcgcctgg cctttggcgg cctgctcctg aaaactcatg cccgtccatt caaggccaaa
      421 gcccggcagc tcatgaacca tgtcggtcat ggcatccatc gcctggccgg aactgtaacc
      481 ttcccctgaa tcgccgagaa aagtcgccga ctcatacata ttgtaacggg tgatggtctg
      541 tgggccgaaa gacggcttgg cgacaagaag tgcgttgagc ggcaccatgt ttccgttttt
      601 gtcacgcacg tcaagcaggc cgacgtcttc aggattcacg cgatattggc taagtgcctg
      661 catcttgacc tgaaaagttt tgccgtaaag attgaagtca ttcacgtaaa gcgagccgag
      721 ataaacctgc agcgtatcaa aaacatcacc gagcgctatg ccctgcgtca tgaccttatc
      781 gcggtcgata tccaggtgta tctgcggcac attcgcacgg aaagtcgtaa aaacgtcttt
      841 gacttccgga ttgccagccg ccatctgcat tagcctgacc gaagcagcct gcaagtcggg
      901 aagcggcata ttcccataat tctgcaactg catctgtaat ccgccagtcg tacccaaacc
      961 gggaatcgtc ggcggcacaa acgacattat cctggcttcc tcgatggttt taaactcgtc
     1021 ggcgagtttt tcaactatcg cattaatctg caattcaggc gttttacgct tgtcccaatc
     1081 atccagcatc acgataagcg acgccgtatt agacgatatg gtactgtcaa tcatggacat
     1141 gccgccgata gatattacgt tggcgacgcc aggcgttttc atgaccatat ccgttgcgcg
     1201 ggtagtcacg gcggaggtac gttcgagcga agccgcatca ggcagctgga tgacagccat
     1261 gaagtaaccc tggtcttcag tggggataaa tcctgtcggc agcgttacga aacccagcca
     1321 cgtcagaacc gtaatgccca aaaacaatat cgctacaatg accagcttgc gaacgagcat
     1381 ttttacaatt ccgccgtaaa accgttccgt tgctgcgaat atcttattaa accagtcgaa
     1441 gaagaaaaat ttagttttgg acgttggccg caacagtacg ccgcaaagcg ccggactgag
     1501 cgtaagcgcg tttacggtgc tgaacgccgt cgacgcggcg attgtcagcg aaaactgctg
     1561 atacagtctg cccgaaatac cgcccataaa cgccgtcggc acgaagaccg acaacaacac
     1621 cagcgtggtt gcgatgcaag ggccgatgac ttcgttcatg gctgcgatag ccgcttcacg
     1681 cggcgcaagg cccagttcgt ctatcttgcg ctgggtgttc tcgacaacca caatggagtc
     1741 gtcgactact atgcctatcg ccagcaccaa tccgaacatc gtcagagtat tgatggaaaa
     1801 tcccaacagt gccatgacga taaatgtgcc tatcagcgaa accggaatgg taagcgccgg
     1861 aatcagcgac gtccgccaat cctgcaggaa tacgaacgtt atcagcacca ccagccccac
     1921 agcctgaaac agcgtgacgg taatctcatt aatcgaggca tggatgaacc acgtgctgtc
     1981 gtaagttacg ttgtacttga ggtctttggg gaagcgcgag gccaatcgct caagctcagc
     2041 cttgcagccc ttttcaacat cgatggcgtt cgagccgggt aactgataga ttgcgatggt
     2101 ggcggacggc ttaccgttat acgtagaata tgaattgtaa agctgactgc cgagttcgac
     2161 cgtgccgatg tcttttattt tcaccggatg gccatccggc ccgactttaa cgacaatgtt
     2221 tttaaattcc tcaggcgtac tgagacgtcc cgtgacggta acggtatatt ggaactcagt
     2281 gcctttcggg cacggcggct ggccgagatt gccggctgcc acctgcacgt tctgcgagcg
     2341 aaccgcgtca gtcacatcgg tgactgtcaa ctgcctggca tgcatggcgt ccggattcag
     2401 ccatatccgc atgccataat ccttgctgcc gaaaatggtg acgttgccca cgccaggcac
     2461 acgggcgagg gtgtcaacca tattgattga ggcgtagttg ctcaattcca aatcgttata
     2521 ggtattattc ggcgagtaaa gattgacggc taagacgaag ttggtggatt ttttctgcgt
     2581 cgacacgccc tgcctttgaa cttcgtccgg cagttgcggg gtggcgatgg taacctgatt
     2641 ctgcgtcagg acggtagcca tgtccagatc catgccgact tcaaaagtga ccgtcagcgt
     2701 atacgtgccg tcgttggcac acgtgctgga catatatatc atgccctcga cgccgttgac
     2761 ctgttcctct atcacctgcg cgacggtatc attgagcgtc acagcgtcgg cgcctggata
     2821 ggtcgtcgtt actgtaactg tcgggggcgt tatgtcaggg taaaggtcta tcggcagcgc
     2881 cgacatggtg acaaggccgg cgatgatgat gacgatggaa atgaccgccg ccagtacagg
     2941 tctttcaatt atgaagcgac tgtacatggt tagcctttct tcttcgcctg aactggtttc
     3001 gtcgtcggct gtgattttac agcggctggc ctggcctgcg gcgcagctgc cgctttttca
     3061 tccgtactgc cagggttgaa aatacccggc
//
LOCUS       DAONFI010000292         3085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_39689_length_3085_cov_4.374587, whole genome shotgun sequence.
ACCESSION   DAONFI010000292 DAONFI010000000
VERSION     DAONFI010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39689_length_3085_cov_4.374587"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="PLK08_08770"
     CDS             <1..1829
                     /locus_tag="PLK08_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53632.1"
                     /translation="PFAAWARLGRLFWESGRKATLARKNDNKEAMFEALSRGTGSSMR
                     LPDWAKPSSPQPFEHPEMLEQPTKQKAAGKSEPQAVPEITIKIKAAAAGIARKLKSLK
                     TRLDEKRLAAAERRLAAIEKARQDAEIRMFEAEQAQLKAEEEELVMAAATAAERQEAQ
                     PQPNETNAADTSDADEQASTSHDEAFSPKKVAEDDFFEEEEQPAAATEEYETGEQAEA
                     DESAKNVDEAEDADQNFPAEEETPFEAEEQSLFTSLQTEEEQTKESDDAEEFIEEIVI
                     EEVEFEEAAETKPTPAENRQPPKHEKPQQPGEEAGLKTDEIKIPPSVSADDSQKSGQP
                     AGDSSQDNDVAAGKTRPIEVIPSVTATSIQQQVNLAEDNALISQTLSARQAALETTPE
                     DSVHITISRRAFIGIIGSALGIIIVVFLLGILISGGDSPKKPAAEPAAEENTPYQAQP
                     QPSQETEPAEETAVAEEKPVKETPSADETPADTQDAPLVIAEENKRDPKRYYIVIQQL
                     LSTGAKDRTEALNIAEFCSERGLPAEAVILDGKKLVVWSLAGLKSTDDEVARKHALDV
                     EKLGKEYFKINRTYDFRQRMRDGRLRPYYIKGKPYATSADGK"
     gene            1863..2024
                     /locus_tag="PLK08_08775"
     CDS             1863..2024
                     /locus_tag="PLK08_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HPS53633.1"
                     /translation="MSIDSSLKKRGSLRGKRSVLTRAERIEKMKAAGKFNPTETSPLG
                     MPKCRVEKD"
     gene            2489..>3085
                     /locus_tag="PLK08_08780"
     CDS             2489..>3085
                     /locus_tag="PLK08_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS53634.1"
                     /translation="MSLEPLQIASRMDKLDASGIRKVFDLAAKLKDPINFSIGQPDFD
                     VPEPVKARAIEAIKQGKNSYTQTQGTAALRGALAELCRREFGWADAHERAYLVAAGVS
                     GALHLAIQVLVNPGDEVIFTDPYFVMYAHLVNLAGGVPVTVDTYEDFRIDLNRLEDAI
                     TPRTRLLILNSPANPTGMVYTREELQGIAQIARKHDLLI"
BASE COUNT          948 a          751 c          805 g          581 t
ORIGIN      
        1 taccattcgc cgcctgggca cggctgggta ggcttttctg ggaatcgggc aggaaggcaa
       61 cgttggccag aaaaaatgac aataaagaag cgatgttcga agcattaagt cgcggcacag
      121 ggtcgtcgat gcggcttccc gactgggcaa agcccagctc gccccagcca tttgaacatc
      181 ccgaaatgct ggaacagccg acaaaacaaa aagcggcagg aaagtccgaa ccgcaggctg
      241 taccggaaat tacgataaaa atcaaagcgg cggctgcggg aatagcccgc aagctgaaaa
      301 gtttgaaaac caggcttgac gaaaaacggc ttgcagccgc ggaacgcagg ctggcagcta
      361 tagaaaaggc tcgccaggat gccgaaatcc gcatgtttga agccgaacag gctcaactga
      421 aggctgagga agaagaactc gtcatggcgg ctgctacggc ggcggaacgg caggaagctc
      481 agccgcaacc gaacgaaaca aacgcggcag atacttccga cgctgatgaa caagcttcaa
      541 cttctcacga cgaagctttt tcgccaaaaa aagttgctga agatgatttt ttcgaagaag
      601 aagaacagcc agccgccgcg acagaggaat atgaaaccgg cgaacaagcc gaagcggatg
      661 agtctgctaa aaacgtggac gaggctgaag acgcagacca gaattttccg gcagaggaag
      721 aaacgccttt cgaagcggaa gaacagagtc tcttcacatc gcttcagact gaagaagagc
      781 agacgaaaga atctgacgac gccgaagagt tcatagaaga aattgtcata gaagaagttg
      841 aatttgagga agcggctgaa acaaaaccaa cgccggctga aaacagacag ccgccaaaac
      901 acgaaaagcc gcaacagcca ggcgaagagg ccggcttaaa aacagatgaa ataaaaatac
      961 cgccatccgt ctcagccgac gattcacaaa aatccggaca gccggcaggt gattcgtcgc
     1021 aagacaacga cgtcgcggca ggcaaaaccc ggcctatcga ggtcattcca agcgtaacgg
     1081 cgacctctat acagcagcag gtaaacctgg cggaagataa cgcactgata tcgcagacgc
     1141 tttcagccag acaggcagcg ttggaaacta cgccggaaga ctccgtgcat ataaccataa
     1201 gccgcagggc atttataggc ataataggca gtgccctagg tataatcatc gtggttttcc
     1261 tgctgggcat actgatatcc ggcggcgatt cgcccaagaa gccggcggct gaaccggcgg
     1321 cagaggaaaa tacgccttac caggcacagc cgcagccgtc gcaggagact gagccggcag
     1381 aagaaaccgc cgtcgcggaa gaaaagcctg ttaaggaaac gccatctgcc gatgagacgc
     1441 ctgccgatac gcaagacgcc ccgctggtca tagccgaaga aaacaaaagg gacccgaaac
     1501 ggtattatat tgtaatacag cagcttttgt ccaccggcgc aaaagaccgc acggaagcgt
     1561 tgaatatcgc agaattctgc agcgaacgag gcctgccggc ggaggcggtc atattggacg
     1621 gcaaaaaact ggtcgtctgg agcctggcag gcttgaaatc tacagacgac gaagtggcaa
     1681 gaaaacatgc ccttgacgta gaaaaactgg gcaaggaata ttttaaaata aacaggacgt
     1741 atgatttcag gcagcggatg cgtgacggaa ggctgaggcc ttactacata aaaggcaaac
     1801 cttacgcaac atcggctgac ggcaaataaa gataatacga ttttttacag gagttcgtga
     1861 aaatgagcat agacagttca ctgaagaaac gcggcagcct ccgcggcaaa cgtagcgtgc
     1921 ttacccgtgc ggaacgtatc gaaaagatga aagccgccgg caaattcaac ccgacagaaa
     1981 ccagccctct gggcatgccc aaatgccgcg ttgaaaaaga ctgattcacg caaaacagtc
     2041 accacggatt tttaaaacaa ttttttaaca tttttttaca attgctcaaa gtgaccggtt
     2101 taacgttata aaaggtcagt aaagttttta aaaaacttca aaaaacttca aaaaatttta
     2161 aagttttgat tttttgattt tttgattttt ttaaaaaaga cttatacacg gtcgcgatat
     2221 tcatggcaaa tggttcatgc gatattgcca ttgaaaactg cggtaatgta ttttattgca
     2281 aaataagccg ttatctttcg cagcaaatgc aaaactgtta aatcggtcag cttgataaca
     2341 ataagtccga aattctggag ggtgaatttc ggatttattg ttattcagaa tggttgtcgt
     2401 atgatattga gggcaatcgt catcacgacg ttggaataat gcggggccgc cattttgaaa
     2461 tgtcaacgag ataaggaaat aatggaaaat gtcacttgaa ccgcttcaga tagcttcgcg
     2521 tatggacaag ctcgacgcat cgggcattcg taaggttttc gacctggcgg cgaagctcaa
     2581 agacccaata aatttttcca taggccagcc ggatttcgac gtacccgaac ctgtaaaggc
     2641 tcgcgccatc gaagccataa agcagggcaa aaacagttat acgcagaccc agggcacggc
     2701 agcccttcgc ggcgcgctag ccgagttgtg caggcgtgaa ttcggctggg ccgacgccca
     2761 cgaaagagcc tacctggtgg cagccggcgt aagcggggcg ctgcatctgg cgattcaggt
     2821 actggtcaac cccggcgacg aagtgatatt caccgaccct tattttgtca tgtacgcaca
     2881 tctggtgaac ctcgccggcg gcgtgccggt gacggtagac acatacgaag acttccgcat
     2941 agacctcaat cgtctggaag acgccataac gccgcgaacc agactgctga tactcaactc
     3001 gccggctaat ccgacgggca tggtctatac ccgcgaggaa ctccagggca tcgcacaaat
     3061 cgccagaaaa cacgacctgc taatc
//
LOCUS       DAONFI010000293         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_39912_length_3073_cov_4.500994, whole genome shotgun sequence.
ACCESSION   DAONFI010000293 DAONFI010000000
VERSION     DAONFI010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39912_length_3073_cov_4.500994"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLK08_08785"
     CDS             <1..301
                     /locus_tag="PLK08_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPS53635.1"
                     /translation="EPVPVFVKGDGREHMVAIEVKKLHEIRPGDILQTQPDDGALPAE
                     VNVGVVDRVEDDAQNAGMVKLYIKPLTNMAALREVMIVIPALGRLETVGHRGTQR"
     gene            298..858
                     /locus_tag="PLK08_08790"
     CDS             298..858
                     /locus_tag="PLK08_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53636.1"
                     /translation="MIRWIPFLLLTYVLVLVQTTVGRILTMEQFSVGPFGPDLLIPLA
                     VFVAFYAKDVYDAMLCGWVIGIFIDLTTAGGGGAPTRVGPMAIILALAMWVTFNARDW
                     LYREKVLPQFIVGGLLCFTTHFLWCVVQIFFASRGWGLLGSLCIQVLLSSLYTALLTP
                     PAFMLLWLCRKWLFAMSTSSRSRRNR"
     gene            878..2842
                     /locus_tag="PLK08_08795"
     CDS             878..2842
                     /locus_tag="PLK08_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPS53637.1"
                     /translation="MYKFRLKILLSLIIIVFLAMLGRLVQMQLVEGHKYREKAKRSLQ
                     TFSLIPAQRGRIFDRNGAILATDEACHDFCMDYRFMTANQPWRDRQIRNIAKKEDISR
                     QRATEEFESREAYTWQLAEQAAQQSGEDLRESVGGIRRTIERMKNRTGRNIREEIVSH
                     PVIKGLTESQVNALRPAMEKTVGVSLTPSHKRFYPFADVACHIIGTTRRVFYEDLQQR
                     NVNDEDVDWLERMKRNYAPTDTIGESGVERACEEQLRFTRGYKRENYSGDVLAYEPPK
                     NGKDVQITLDIELQRVLTKIFADTKHTGSIVVMDVPTGEILAMVSWPTYDNNNFRQLY
                     PQLLADKVNLPLHHRAVAATYAPGSTIKPFTGLIASELGRLSADEIITCTGENRLARN
                     GKPRCFIYKEYHTTHGDLTLSDALKVSCNIYFVTAADRIGSAMLAQKLHDFGFGEYPH
                     TGLPEERDGIVGDETYLRKNFNRGFYRSDAWFIGMGQGVFTASPIQIAAAVATIARDG
                     VYLSPKLMRGDEFPQKVRQIPIPQHTLDMVREGMGRVVNEQGGTAYKTWNLGEVPDVT
                     VCGKTGTAQTPPMRIDSNHDGKITTDDTIVKTGDMSWMMAFAPKDNPQIAVSVLVEYS
                     DGGGGADCGPIVKEVVRQCEKFGYIKGAAQ"
     gene            2839..>3073
                     /locus_tag="PLK08_08800"
     CDS             2839..>3073
                     /locus_tag="PLK08_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53638.1"
                     /translation="MSKTGPRLIGLFIMLAMMLLMTIGISAIHTSELAEGVSSGYCEK
                     QIIFAAIGIAVFFAFSVIPYPRFGQIAYAFFGFS"
BASE COUNT          764 a          836 c          872 g          601 t
ORIGIN      
        1 cgaacctgtg ccggtgttcg taaaaggcga cggccgtgaa cacatggtgg ctatcgaggt
       61 gaaaaaatta cacgaaatac ggcccggcga cattctgcag acccagccgg acgacggcgc
      121 gctgccggct gaggtgaatg tcggcgtggt agaccgcgta gaagatgacg ctcaaaacgc
      181 cggcatggtc aagctgtaca tcaagccgct gacgaacatg gccgcactgc gggaagtgat
      241 gatagtaatt ccggcactgg ggcggctgga aactgtgggc cacaggggga cgcaacgatg
      301 atacgatgga taccgtttct gttgctgacc tacgtgctgg tactcgtgca gacgacggtc
      361 ggccgcatac tgacaatgga gcagttcagc gtcgggccgt tcgggccgga tttgctgata
      421 ccgctggcgg tgttcgtggc gttttacgcc aaagacgttt acgacgcgat gctctgcggc
      481 tgggttatcg gcatattcat agacctcaca accgccggcg gaggaggcgc gccgacgcgg
      541 gtagggccga tggctataat actcgcactg gcgatgtggg ttacgttcaa cgcacgcgac
      601 tggctgtatc gcgaaaaagt tctgccgcaa ttcatagtcg gcgggctgct gtgtttcacg
      661 acgcatttcc tgtggtgcgt ggttcagata ttttttgcat cgcgaggctg ggggctgctg
      721 ggcagcctct gcatacaggt actgctgagc agcctgtata cggcactgct tacgccgccg
      781 gcattcatgc tgctgtggct gtgcagaaaa tggctgttcg ccatgagcac gtcgtcgcgt
      841 tcgcggcgaa accgttgata ccggagattt ttcagaggtg tacaaattcc gtttaaaaat
      901 cctgttaagc cttataataa tcgtcttcct tgcaatgctg ggacggctgg tgcagatgca
      961 gcttgtcgaa ggtcacaaat acagggaaaa agccaaacgc tccctgcaga cgttctcatt
     1021 gataccagcg cagcgcggcc gaattttcga ccgtaacggc gcaatcctcg ccaccgacga
     1081 agcctgccac gatttctgca tggactaccg cttcatgacc gccaatcagc cgtggcgcga
     1141 ccgacaaata cgaaatatcg ccaaaaagga agacatctca cggcagcggg ctaccgaaga
     1201 atttgaatcc agggaagcat atacctggca gctcgccgaa caggcggccc agcagagcgg
     1261 cgaagacctc cgcgaaagcg tgggcggcat acgaagaacc atcgaacgga tgaaaaatcg
     1321 caccggcaga aacatacgcg aagaaatcgt ctcccatccg gtgattaaag gccttaccga
     1381 atcgcaggtg aacgccctgc ggccggcgat ggaaaaaacc gtcggcgtat cgctcacgcc
     1441 cagccacaaa cgattctacc cattcgccga cgtcgcatgt cacatcatcg gcacaacgcg
     1501 gcgggtcttc tatgaagacc tacagcagcg aaacgtaaac gatgaagacg tcgactggct
     1561 ggaacgcatg aaacgcaact atgcaccaac agacaccatc ggagaaagcg gcgtcgaacg
     1621 cgcatgcgag gaacagttgc gattcacccg cggctacaag cgggaaaact acagcggcga
     1681 cgttctggcg tatgagccgc cgaaaaacgg caaagacgta caaataacgc tggatatcga
     1741 actgcagcgc gtgcttacca agatattcgc ggacacaaag cataccggca gtatagttgt
     1801 catggacgtg ccgactggcg aaatactcgc catggtatca tggccgacgt acgacaataa
     1861 caatttccgc caactgtatc cgcaactgct ggcggataag gtcaatctgc cgttacacca
     1921 tcgcgcagta gcggccacgt atgcccctgg ttcgacaatc aaaccgttca ccggccttat
     1981 cgcgtcggaa ctgggcaggc tatccgccga tgaaataata acctgcaccg gcgaaaaccg
     2041 gctggcccgc aacggcaagc cgcgatgttt tatctacaag gaatatcata cgactcacgg
     2101 cgaccttacg ctcagcgacg cactgaaagt gagctgcaat atttatttcg tgacagccgc
     2161 cgacaggata ggctcggcaa tgcttgccca aaaactgcac gacttcggtt ttggcgaata
     2221 tccacacacc ggcctgccgg aggaacggga cggcatcgtc ggcgatgaaa cgtatctgcg
     2281 caaaaacttc aatcgcggat tctatcgcag cgacgcatgg ttcatcggca tgggccaggg
     2341 cgtattcaca gcatcgccga ttcagatagc cgccgcagtc gcaaccatcg cgagagacgg
     2401 cgtatatctt tcgccaaaac tgatgcgcgg cgacgaattt cctcagaaag tcagacagat
     2461 tcctattccg cagcatacgc tcgacatggt gcgggaaggc atgggcaggg tcgtcaacga
     2521 acagggcggc actgcatata aaacgtggaa tctcggcgaa gtgccggatg ttacggtctg
     2581 cggcaaaacc ggcacggctc agacgccgcc gatgcgtata gactccaacc atgacggcaa
     2641 aatcacgacc gatgatacga ttgtaaaaac tggcgacatg tcgtggatga tggctttcgc
     2701 gccgaaagac aatccgcaga ttgcagtctc cgtactggtg gaatattccg acggcggcgg
     2761 cggtgcggat tgcgggccta tcgtcaagga agtagtaagg cagtgtgaaa aattcggcta
     2821 tatcaagggg gcggcgcaat gagcaagacc ggcccacggc tgattggcct gtttatcatg
     2881 ctggcgatga tgcttctaat gaccatcggc atctcagcca tccatacatc ggaactggcg
     2941 gaaggcgtat cgagcggtta ttgcgaaaag cagataatat tcgccgccat cggcatagcc
     3001 gtattcttcg cattcagcgt cataccttat ccgcgtttcg gccagatagc gtatgccttt
     3061 ttcggctttt cac
//
LOCUS       DAONFI010000294         3070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_39971_length_3070_cov_5.978109, whole genome shotgun sequence.
ACCESSION   DAONFI010000294 DAONFI010000000
VERSION     DAONFI010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39971_length_3070_cov_5.978109"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLK08_08805"
     CDS             complement(<1..286)
                     /locus_tag="PLK08_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53639.1"
                     /translation="MTDSNTNSTTFQPGHTIGRYEVTAVLGIGGQGVVYRCKDPILGR
                     DVAIKHIATHLIADPTYRQRVMQNINNLARLGEKSDAIVTIHDMFETEQGL"
     gene            complement(293..832)
                     /locus_tag="PLK08_08810"
     CDS             complement(293..832)
                     /locus_tag="PLK08_08810"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPS53640.1"
                     /translation="MQATWHYWVLGGMLIAAGIDDVVAGKVHNVITLPCLAAGLLGHA
                     AISGGMSLQDAIIGMAAGFLPAFVAWKFGAIGGGDVKLLAAVGALAGWRFTITAMFFG
                     LCVAAVAAVAMLVAKKQLSATFRRIGFFAYMAAAGANPPSPTDQTSRKLPLGLALCVG
                     CAIVMIIELAGLHCRFIWM"
     gene            complement(832..1044)
                     /locus_tag="PLK08_08815"
     CDS             complement(832..1044)
                     /locus_tag="PLK08_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HPS53641.1"
                     /translation="MKLYGKIRNELRSLMKDEQGATMIEYILIVAAIALPLLAVILWF
                     KDDISKWFGEQYTDVKSGNASDDPTD"
     gene            complement(1041..2162)
                     /locus_tag="PLK08_08820"
     CDS             complement(1041..2162)
                     /locus_tag="PLK08_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPS53642.1"
                     /translation="MSKGNLNIFEATVEHFLAPVMEYMHDDTVSEIMINSANDIYIER
                     AGKLIKTNAGFEDEESLLAAVNNVLQYTGKRIDPERPLVDSRLPDGSRVHVVLSPLCR
                     NGTCMSIRKFSRLNLNADHLLQYGSWTKEVMDYLRVCVLAEKNLLVAGGTSSGKTCLL
                     NVISSFIPHDQRIVTIEDSAELELQQSHVVSLESRPPDRWGKGEITIRDLFRSSLRLR
                     PDRIVIGEVRGGEALDMIQAMTSGHAGSMSTLHANTAFDALNRLETMALMSKVELPLH
                     ALRSQISSAMDVIVLMTRLKDGRRCITEISEVLPLEEDGHYRVQKMFSFDVKDESDVG
                     RLAWAGQRSTFYDEPKIRINRPEWRDAAKFFSQQTGENT"
     gene            complement(2576..>3070)
                     /locus_tag="PLK08_08825"
     CDS             complement(2576..>3070)
                     /locus_tag="PLK08_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53643.1"
                     /translation="APLPADHPQAWRVYNERVMRLVNNLTESLNPDKTVSHGLVLLRT
                     QTTQNHTRRALSVARTYYEVIRSGWALVAISKNGLDMLLMGPNSALNEEILSDIRNKI
                     KYDPDIYLVPIEELWKAYPGVAPIRIFDPNGERLDDSPQLEGLQNYLGKTRTVLEMNQ
                     KKEN"
BASE COUNT          639 a          823 c          805 g          803 t
ORIGIN      
        1 acaggccctg ctcggtttcg aacatgtcgt ggatggtgac tatggcgtcg gacttctcgc
       61 ccagccgcgc aaggttgttt atgttctgca tgacccgctg acggtacgtg gggtctgcaa
      121 tcagatgggt tgcgatgtgc tttatggcga cgtccctgcc aagtatgggg tctttgcagc
      181 gatagaccac tccctggccg cctattccga ggacggcggt gacttcatag cggcctatcg
      241 tgtggccggg ctgaaacgtt gtcgaattgg tattgctgtc agtcattttt gattacatcc
      301 atataaaacg acagtgaagt cctgcaagtt cgattatcat gactattgcg catccgacgc
      361 atagtgcaag gcccagcggc agtttgcggc tggtttggtc tgtcggcgag ggcggatttg
      421 cgccggctgc agccatgtat gcgaagaaac ctattcttcg gaatgtggca gacagctgtt
      481 tttttgctac gagcatggcc accgccgcta cggctgcgac gcacagtccg aagaacatgg
      541 ctgttatcgt gaatcgccag ccggcgagtg cgccgacggc ggctaacagt ttaacgtctc
      601 caccgccgat tgcaccgaat ttccacgcta cgaacgccgg cagaaatcca gccgccatgc
      661 cgatgatggc atcctgcagg ctcatgccgc ccgatattgc cgcatggcca agcaggccgg
      721 ctgccaggca cggcaaggtt atgacgttgt gaaccttgcc tgcgacgacg tcgtcaatgc
      781 ccgccgcaat cagcatgccg ccaagcaccc agtaatgcca cgtagcctgc atcaatcggt
      841 cgggtcgtcc gatgcgttgc cgctctttac gtcagtgtac tgctcgccga accatttgct
      901 gatgtcgtcc ttgaaccaca ggatgaccgc aaggagcggc aatgcaatcg ccgccacgat
      961 gagtatgtac tctatcatgg ttgcaccctg ttcgtctttc atcaggctgc gaagttcgtt
     1021 gcgaattttt ccatacaact tcatgtattt tctcctgtct gctgcgagaa aaactttgcc
     1081 gcgtcccgcc attcgggccg gttgattcgt attttcggtt catcgtaaaa ggtgcttctt
     1141 tggccagccc acgcaagtcg gccgacgtcg gattcatcct tgacgtcaaa cgagaacatt
     1201 ttctgcaccc tgtagtggcc gtcttcttcc agcggcagca cttcggatat ctccgttatg
     1261 cagcggcggc cgtctttcag tcgcgtcatg agcactatca catccatcgc cgatgatatc
     1321 tgactgcgca gggcgtggag gggcagttca accttgctca tgagggccat cgtttccagc
     1381 cggttcagcg cgtcaaacgc ggtgttggcg tgcagcgtgc tcatgctgcc ggcgtggccg
     1441 gaggtcatcg cctgtatcat gtcgagcgcc tcgccgccgc gaacttcacc gatgactatc
     1501 ctgtccggcc gcaaacgaag cgatgaccgg aaaagatcgc gaatcgttat ttcgcccttg
     1561 ccccagcggt caggcggtcg ggattcaagc gaaaccacgt gcgactgctg caactcaagc
     1621 tcggcggagt cttctatggt aactattctc tggtcgtggg ggatgaaact gcttatcacg
     1681 ttgagcaggc aggtcttgcc gctggatgtg ccgcctgcca ccagcagatt cttttccgcc
     1741 agcacacaaa ctcgcaaata gtccatgacc tcttttgtcc atgagccgta ttgcagaagg
     1801 tggtcggcat tgagattcaa tctcgaaaat ttgcgtatcg acatgcatgt gccgttcctg
     1861 cagagcggcg ataacacgac atgcacccgg ctgccgtcgg gcagacggga atccacgagc
     1921 ggcctttcgg gatctatgcg tttgccggtg tattgcagca cattgttgac ggcggcaagc
     1981 agggattctt catcttcgaa gccggcgttg gtcttgatga gtttgccggc tcgctctatg
     2041 tatatgtcgt tggcggaatt aatcattatc tcggagacgg tgtcgtcgtg catgtattcc
     2101 atgaccggag caaggaaatg ctccaccgtc gcctcaaaaa tgttcagatt gcctttcgac
     2161 accacaaaat ctccatattt cccgtttaac gccggaaacg ataaaatatt aaaaacattg
     2221 caaccgtctc caaaacggaa tcttacgtca aaaatagcgc aaaatccagc tttttattac
     2281 atcctcacag aaaaccactg gcttgccgga aggtctctta ttgaatcttg agaattggaa
     2341 gttgcttgta attttgtatc ttgcaacttg tgtagtttat agcggttaaa aacagttcag
     2401 agtcggtaca ggatgctaga gcagttcaat cttgagagtt ccttttttgc tggtttgctt
     2461 tgtccgtggc tgcgtcagaa aaacttcaga atactgccaa gtatgcctca ttttccttcc
     2521 ttgccacgaa caaacccact ggcgcaaaaa tatgaactct caatctttca atgctctaat
     2581 tttccttttt ttggttcatt tccagtacgg ttctggtttt tcccaggtaa ttctgcaagc
     2641 cttccaactg cggcgaatcg tccagtcttt ccccgttggg gtcaaatatc cgtatcggag
     2701 cgactcccgg atacgctttc cacagttcct ctatcggcac gagatatata tccgggtcgt
     2761 acttgatttt attacgtatg tccgagagta tttcttcgtt aagggccgaa ttcgggccca
     2821 tgagaagcat gtccagcccg tttttgctta tcgcaaccag cgcccagccg ctgcggatta
     2881 cttcataata tgttcttgcg acgctaagtg cgcggcgggt gtgattctgg gtggtttgag
     2941 ttcgtagcag aacaaggccg tgcgatacgg ttttatcggg attgaggctt tccgtgagat
     3001 tgttcaccag tcgcatgacc cgctcgttat atacccgcca ggcttgtgga tggtctgccg
     3061 gcaggggggc
//
LOCUS       DAONFI010000295         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_40066_length_3066_cov_2.941216, whole genome shotgun sequence.
ACCESSION   DAONFI010000295 DAONFI010000000
VERSION     DAONFI010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40066_length_3066_cov_2.941216"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLK08_08830"
     CDS             complement(<1..458)
                     /locus_tag="PLK08_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPS53644.1"
                     /translation="MSELLKLAAENLPKLARRPRDGHKGVFGKILVLGGSRRMIGAPA
                     LAGQGALRGGAGLVTIATPGPVQLAVAMLCECATTVPLACDTSNRLAQQALSQFSRAI
                     DACDVIVAGCGFAVGTEQQNFIRMIIEQSKPVVLDADGLNNLSQISDWPSL"
     gene            complement(451..897)
                     /locus_tag="PLK08_08835"
     CDS             complement(451..897)
                     /locus_tag="PLK08_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53645.1"
                     /translation="MNNEKWQPVSGPSGNGQNDYDSNRKAEYSMGDTSLEELRKTYQN
                     MRTMRKVIEKNTTHWKTTFMLAGSLVGSMLYLVGLIQGGLSYPEGIHLATLSAIVGLM
                     VFGLFFPLFVCLYFSRGFLDFIKRIEDKVFPNDPVFRGVQEEDEDE"
     gene            complement(985..1740)
                     /gene="larB"
                     /locus_tag="PLK08_08840"
     CDS             complement(985..1740)
                     /gene="larB"
                     /locus_tag="PLK08_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPS53646.1"
                     /translation="MDEISLKKMLEEYRLGRLNQQEVISKLQTLPFESLSHARVDHHR
                     KLRCGYGEVIFCQNKTPQQVCEIFARLAAEGDVLATRATAEMYEAVKKVCAGAVFYPI
                     SRAIIMMKEQKRPCGLVAIVSAGTSDMAVAEEARLTAEFMGAEVSCHYDVGVAGLHRL
                     LADLDDIRKANAVVAVAGMEGALASVIGGLVSVPLIAVPTSVGYGASFGGVAALLAML
                     NSCAAGVSVVNIDNGFAGGYNAALINRLAVRKR"
     gene            1890..2999
                     /locus_tag="PLK08_08845"
     CDS             1890..2999
                     /locus_tag="PLK08_08845"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPS53647.1"
                     /translation="MNSESDVKKHHSMPKLVGRGVFGGFLMGMANLVPGISGGTMLVA
                     AGIYKDFIDAIAEVTTFRCRLRSMVLFLSVVIAAAAAIVVFAGMVKGLVVDCRWVMYS
                     IFIGLTLGGVPVIWKMIKGGAGAGFWIGAAVGLACMAVLAWYQQKNHGAICSAEGFGF
                     IFMAGIIGAVAMILPGISGGYMLLLLGVYVPVLGAIDACKIALKTHELAAAKEPFIQY
                     ILPMTLGVILGIVVISNILKMVLAKFEKPTLGVLMGLLIGAVMGLWPFQQGVVPKAGD
                     VLKGEQVIVIQSGDVINGNMVVEIDREIFVLDGSRKVKYPAGLALAVSGKPIPQDKYP
                     TEFFKPAMWQAFAAAGLVLAGFFTTFVISRISADE"
BASE COUNT          719 a          755 c          826 g          766 t
ORIGIN      
        1 agcgatggcc agtcggaaat ctgcgaaaga ttgttcaatc cgtcggcgtc gaggacgacg
       61 ggttttgact gctctataat catgcggatg aagttctgct gttccgtgcc gacggcgaaa
      121 ccgcagccgg cgacgatgac gtcgcatgcg tcgattgccc ttgaaaattg cgaaagagcc
      181 tgttgtgcga gtcggtttga cgtatcgcag gctaacggca cggttgtggc gcattcgcag
      241 agcattgcaa cggcaagctg aactgggccc ggggtagcta tggttacgag gcctgccccg
      301 cccctgagag ctccctggcc tgccaacgca ggcgcgccaa tcatccggcg agagccgccg
      361 agtacgagaa tcttcccgaa aacgccttta tggccgtcgc gcggacgcct ggccaacttg
      421 ggcagatttt ccgcggcgag cttcaataat tcactcatcc tcgtcctctt cttgcacgcc
      481 gcggaagacg ggatcgttcg ggaatacttt gtcttcgata cgctttatga agtccagaaa
      541 accgcgggag aaatacaaac acacaaatag cgggaaaaac agcccgaaaa ccataagtcc
      601 aactattgcc gagagagtcg ccagatgtat tccttcggga tagctcaggc cgccctgtat
      661 cagtcccacc aggtatagca tggaacctac cagtgaccct gcgagcatga acgtggtctt
      721 ccagtgtgtg gtattttttt ctatgacttt tcgcatggtg cgcatattct gatacgtctt
      781 gcggagttct tcaagacttg tgtcgcccat gctgtattct gcttttctgt tggagtcata
      841 gtcattctgt ccgttgccgg acggccccga cacaggttgc catttttcat tattcataaa
      901 aaattccttt atattttcgg ccatgctgat aatggaaacg ctgtgcggct gtaaaaaaaa
      961 atccaacgcg ttctgtcgtc gaaatcatct tttacgaaca gcgagcctgt ttattaaagc
     1021 ggcattataa ccgccggcga aaccgttgtc tatattcact acgcttacgc cggcggcaca
     1081 gctgttgagc attgccagca gtgctgcgac gcctccgaag ctggcgccgt agcccactga
     1141 cgtaggcaca gcgattaagg gcacgctcac caatcctccg attacgcttg ccaaggcgcc
     1201 ttccatacca gcgacagcca ctacggcgtt ggccttgcgg atatcgtcca ggtctgccag
     1261 cagcctgtga aggcctgcca cgccaacgtc gtaatggcag ctaacttctg cgcccatgaa
     1321 ttcagctgtc agccgtgctt cctcggcgac ggccatgtca ctagtcccgg ccgatactat
     1381 cgccacaagc ccgcaaggac gcttttgctc cttcatcata attatagcgc gggaaatggg
     1441 ataaaacact gcgccggcac aaactttttt aacggcttca tacatttcgg ctgtcgcacg
     1501 ggtggcgaga acgtcacctt cggcggcgag cctggcaaaa atctcgcaaa cctgctgcgg
     1561 agtcttgttc tgacagaaga tgacttcgcc gtaaccgcat cgcagcttgc gatgatgatc
     1621 cacccttgcg tgcgacaacg actcaaacgg cagtgtttga agctttgaaa tgacctcctg
     1681 ctgattcagt cggcccagcc tgtattcttc cagcattttt tttaatgaaa tttcatccat
     1741 ccgcataaag ataccctaaa aaaaggcagt gaatcaagca gtttttggct gattggattg
     1801 cggagagtga attttcttgt tttttagccc ataaaatcta tgatttcagg tttgtaagga
     1861 aattaaacaa ccccaataaa ggaaaatata tgaattctga atctgacgta aaaaagcatc
     1921 atagtatgcc gaaactggtt ggcaggggag ttttcggcgg ttttttaatg ggcatggcta
     1981 atcttgtgcc gggaataagc ggcggtacaa tgcttgtggc tgccggcatt tataaggatt
     2041 ttatcgacgc catcgccgaa gtgaccacat tccgctgtcg tttgcgcagt atggtattgt
     2101 ttttgagtgt tgtaatagcg gctgcggctg cgatagtggt ttttgcgggg atggtcaaag
     2161 gcctcgtggt tgactgccgt tgggtgatgt actcaatatt tatcggcctg acgcttggcg
     2221 gcgtgccggt gatttggaaa atgatcaaag gcggcgcagg ggcgggattc tggataggcg
     2281 cagccgtcgg ccttgcatgc atggctgtac ttgcgtggta tcagcaaaaa aatcacggcg
     2341 cgatatgttc ggctgagggg tttggattca tattcatggc tggaattata ggggcagtcg
     2401 ccatgatact gccgggcatc tcgggcggct acatgctgct gctgctgggc gtatatgtgc
     2461 cggtgctcgg cgcgatagac gcatgcaaaa ttgcccttaa gacgcatgaa ctggcggcgg
     2521 cgaaagaacc ctttatccaa tacatcctgc cgatgactct cggagtgatt ctgggaatag
     2581 tcgtcataag caacatcctg aaaatggtgc tggcaaaatt tgagaaaccg actctcggcg
     2641 tgctgatggg actgcttatc ggtgcggtaa tgggtctttg gccgtttcag cagggcgtag
     2701 taccgaaggc tggagacgtg ctcaagggcg agcaggtaat cgtcatacaa tcaggcgacg
     2761 tcatcaacgg caacatggtt gtggagatag acagggagat atttgttctg gacggcagca
     2821 gaaaggtgaa atatccagcc ggccttgcat tggcggtcag cggcaaaccg ataccgcagg
     2881 acaaataccc aacggaattt ttcaaacctg cgatgtggca ggcgtttgcc gcggcaggcc
     2941 ttgtccttgc cggatttttt acgacatttg tcatatcgcg aataagtgcc gatgaatgaa
     3001 tttttaaatt aactcaaagt gaccgattga cgatatttgg ttcggtaaat actggtttta
     3061 cacaaa
//
LOCUS       DAONFI010000296         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_40098_length_3064_cov_4.090728, whole genome shotgun sequence.
ACCESSION   DAONFI010000296 DAONFI010000000
VERSION     DAONFI010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40098_length_3064_cov_4.090728"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1641)
                     /locus_tag="PLK08_08850"
     CDS             complement(271..1641)
                     /locus_tag="PLK08_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPS53648.1"
                     /translation="MQERFLDELSATATENNRVNPENYDKFEVKRGLRNANGSGVLAG
                     LTVVGDVHGYIFDEGDRVPRPGRLRYRGIDVAAMTAGFRKDDRFGYEETAYLLLFAML
                     PTSEQLERFNKMLGACRKLPENFTEDMILSAPSPDIMNKLARCTLASYSYDRNPDDNS
                     IRNVLRQSMELISRFPTMMAYAYQAKRHYYDSKSLFIHMPKEELSTAENILRLIRPTA
                     EYTMSEAKLLDIMLVLHAEHGGGNNSSFTARVITSADTDTYSAIAGAVGALKGSKHGG
                     ANNRVMGMMTDLKAHVNDWTNEGQLRDYLLKILNKEAYDHSGLIYGLGHAIYTLSDPR
                     AVILKAQAEELATEKGRMDEFNLYKTVEQLAIDTFCEFKKTARTISVNVDFYSGFVYD
                     MLGIPPELYTPLFATSRIAGWCAHRIEEIVSGGRIYRPAFKCVMPPIKYVPLDKRVKG
                     QKPVAE"
     gene            1931..>3064
                     /locus_tag="PLK08_08855"
     CDS             1931..>3064
                     /locus_tag="PLK08_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPS53649.1"
                     /translation="MNDIIVALSSAAGTAERNIVRISGPGALKLAGRIFFHSDKQWNL
                     EEQAGFTFVDGVIKLSGYVQFPCRIYVFRKPASYTRQDIVEFHIPGGLACPAAVMEAL
                     LAGGARAASAGEFTARAFLSGRIDLSQAQAVADVIAAGDEAHLRASLAMLGGRLFKIC
                     RRFADAAAESLALVEASIDLAEEDISLAEPGRVADDLRKCSDGMRELAANAGDMPELT
                     NCPQVVIVGQPNVGKSSLLNALCGRERAIISDLAGTTRDVLREQIALPCGSVVDMIDV
                     AGFGRTGDALCDHAGTAARNAVAQADLMLFVINATCGFTEENRRLFADVREFNPAAEV
                     VCVYNKSDIADEIFACDDKDFAAVIRTSTISGAGLDELKAAITL"
BASE COUNT          647 a          734 c          854 g          829 t
ORIGIN      
        1 cgaacaaacc cactggcgca aaaatatgaa ctctcaatct ttcaatgctc tagcaattag
       61 agcagttttc gaaaaaaaga cttttgaaaa ctgcttttgc gtccgcagga taattgagaa
      121 caacctctaa gtttcatcag atatgggtta ttcgtaaaaa acttgaaact tgtttctttg
      181 acgttacttg taagttgttt tcttgtttct tgaaacttat attatgaccg caactggagt
      241 attatgctct agcattgggg ttctagccgt ttattctgcg actggttttt ggccttttac
      301 gcgtttgtcc agcgggacgt actttattgg cggcatgacg catttaaagg ctggccggta
      361 tatccttccg ccggatacga tttcctctat gcgatgcgcg caccagccgg cgatgcgact
      421 cgtagcgaat agcggcgtat aaagttccgg cggaatgccg agcatgtcgt atacaaaacc
      481 actgtaaaaa tcgacattta cgcttatggt tctggccgtc ttcttgaact cgcagaaagt
      541 gtcgatggcc aactgttcga ccgttttgta aagattgaat tcgtccatgc gtcctttttc
      601 cgtggcgagc tcttccgcct gagccttaag tatgacagcc cgcgggtcgc ttagtgtgta
      661 tatagcgtgg ccgaggccgt aaatcaggcc gctgtggtca tatgcttcct tgttgagtat
      721 tttgaggaga taatcgcgca gctggccttc gttcgtccag tcgtttacgt gagccttgag
      781 gtcagtcatc attcccatga cccgattgtt cgctccgcca tgctttgagc ctttcaacgc
      841 tcctacggcg cctgcgatgg cggaataggt gtccgtgtcg gctgaggtta ttacgcgggc
      901 ggtgaacgac gaattattgc cgccgccgtg ttctgcgtga agtaccagca ttatatcgag
      961 cagttttgcc tcagacatgg tgtattcagc agtcggacgt atcagccgca gaatgttttc
     1021 cgccgtcgat agttcttcct tgggcatgtg aatgaagagg cttttgctgt cgtaataatg
     1081 tctctttgcc tgataggcat aagccatcat cgttggaaac cgcgatataa gttccatcga
     1141 ctgacgcagt acgttgcgga tggaattgtc atccggattc ctgtcgtacg aatagctcgc
     1201 cagcgtgcag cgtgcgagct tgttcattat gtccggactt ggggcgctga gtatcatgtc
     1261 ctctgtgaaa ttttccggaa gtttacggca tgcgccgagc atcttattga atcgttcgag
     1321 ctgctcgctg gtgggcaaca ttgcgaacag cagaagatac gccgtctctt cgtatccgaa
     1381 ccggtcgtct ttcctgaagc ccgccgtcat tgccgcgacg tctatgccgc ggtagcgcag
     1441 ccggccaggc cgcggtacgc ggtcgccttc gtcaaatatg tatccgtgca cgtcacctac
     1501 gactgtcagc ccggcaagca ctcctgaacc gttggcattg cgcagaccgc gtttgacctc
     1561 gaatttatcg taattttccg gattgacacg gttgttttcc gtggctgtgg cggaaagttc
     1621 gtcgagaaaa cgttcctgca tctgcactat gtcggcatcc aagttactag tgggcatggg
     1681 aatatcctgc aaaaaatatg gaaaatccaa aagacactgc actgaggcat ccatccagag
     1741 ttcagttccg agtgacgtat tatacagata cgctgccgga atgtaaaggg ggtttggccg
     1801 tgaggttttt tattatttaa ccgtgtggtt gtcggcggga aacttttgaa ataggttcta
     1861 tttggaagcg gtcagttttt gaatttttga attatggagt agaaaaagat ataattcccg
     1921 catgtttgag atgaatgata ttattgttgc gctttcttcc gctgccggca cggctgagcg
     1981 gaacatcgtg cgcataagcg gccctggagc gttgaaactt gccggccgca tattttttca
     2041 ttcagataaa cagtggaatc tggaagaaca ggctggcttt acttttgtgg acggcgttat
     2101 aaagttgtcg ggctacgtcc agtttccctg ccggatttac gtttttcgta agccggcgag
     2161 ctataccagg caggatatcg ttgaatttca cattcctggc gggctggcgt gtccggcggc
     2221 ggttatggag gcgttgttag ctggcggtgc gagagctgcg tctgccggcg aatttaccgc
     2281 cagggcgttt ttgagcggac ggatagactt gtcgcaggca caggcggtgg ctgacgtgat
     2341 agccgccggc gacgaggctc atctgagagc gtctcttgcc atgctgggcg gccggctgtt
     2401 caaaatctgt cgccggtttg cagatgccgc ggctgagtcc cttgcacttg tggaagcgtc
     2461 gatagatttg gcggaggagg atatttcgct ggctgagccg ggcagggtgg ctgacgattt
     2521 gcgaaaatgc tctgacggca tgcgggaact tgcggcgaat gccggcgata tgcctgaact
     2581 tacgaactgt ccgcaggtgg tcatcgtcgg ccagccgaac gtagggaaaa gttccctgct
     2641 caatgcgctt tgcggacgag agagggctat catatccgac ctcgcaggca cgacgcgcga
     2701 tgtactccgt gagcagattg cactgccgtg cgggtcggtt gtcgatatga tagacgttgc
     2761 cggctttggc cgtaccggcg acgcattgtg cgaccacgcc ggaacagccg ccagaaacgc
     2821 cgtagcccag gctgacctca tgctgttcgt tatcaatgcg acttgcggat ttacggagga
     2881 aaaccgcagg ttattcgccg atgttcggga atttaatccg gcggctgagg ttgtttgcgt
     2941 ctataacaag tcggatattg ccgacgaaat cttcgcctgc gatgacaagg attttgccgc
     3001 cgtaattcgc acttcgacga tttcaggtgc gggccttgac gagctcaaag ccgccataac
     3061 gctt
//
LOCUS       DAONFI010000297         3046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_40420_length_3046_cov_4.808091, whole genome shotgun sequence.
ACCESSION   DAONFI010000297 DAONFI010000000
VERSION     DAONFI010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40420_length_3046_cov_4.808091"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="scpB"
                     /locus_tag="PLK08_08860"
     CDS             complement(<1..777)
                     /gene="scpB"
                     /locus_tag="PLK08_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPS53650.1"
                     /translation="MSEESNEQTDLPAKNEEVCNSDESTAEQMSDVSAAPADADNAPL
                     TPQSEQEVQQAALDEMASTIEAILFSSGSPVPPAKLSTIAELPVRLLKKAILALNERY
                     QKGGCSFRVMNIAGGYQLMTLPEYNDVLKKLLNVHKESRLSQAALETLAIVAYRQPIL
                     RADIESIRGVACGEVLRSLMERQLIKIVGRAEVIGRPMLYGTSRKFLEIFGLGSLEDL
                     PRAEELRQPADKAVKKQEKPEGNQEEKTIDEEKGMKKKFED"
     gene            complement(1010..>3046)
                     /locus_tag="PLK08_08865"
     CDS             complement(1010..>3046)
                     /locus_tag="PLK08_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53651.1"
                     /translation="TWYIILPPAKARFFISSQYVQKIGETSTDTAPESEYEEPAATTE
                     SAETKSTVAVTPETPAVSSTPKRIEEKTATGGSAAVAPDVRLVNKKPEPGVRSESDML
                     RQIRILEKNLMAENAKPMEQRDMHKLLAEAQAINVPDGSRFKPIYDSMVDFIKDEIKI
                     ADETRRTQELVSKTIREVNEPVKPQPAKPQTPAENVVIGKIQPSRVFDRYDSAIGQIY
                     ILRNPNTGEILTFARPADSTIDVNAYIGQVVRISGRKDFDRKAGVQIIKISDISPASA
                     EDAVAKIAVEEQPESARPATNISEPQQEQQTEPATAENVAVKTPAVPAAKAQNENEAG
                     QLTPEDLGMAKGISKPSIEDIDLPASSKQAQTPAAQTKPAESMKDVAIKKPELPPVES
                     LPQPAPKTPEVAEKPSIPTPATVEPVEQPSTVVKPIASVTPEEEGPAPVEVNKIAAPN
                     PVAATKESEAANVESPKIPEVETLEPSPTVAIEPKKTSQEAIPAVPEVKSLDAPVAID
                     TETEKPATKEIAVPPVVVKTPEVKPIEAPAVPTDITPKTAVAQTPSTPDLKQLTPPSL
                     DENTTEVVKIDEEKLPTAPALKSITAPSENETAENESTETVKPIETPMAEETAIAKTT
                     AAGPDIAAIPVPAENPAEATHRPKALKNVAVPTADMPIKGSATPVESMYEVEWD"
BASE COUNT          560 a          754 c          811 g          921 t
ORIGIN      
        1 atcttcaaat tttttcttca tccctttttc ctcatcaatg gttttttctt cctgattgcc
       61 ttcaggtttt tcctgttttt taactgcctt atcagccggc tgtcgaagtt cttccgcccg
      121 cggcagatct tcaaggctgc ccaggccgaa tatttccaga aattttcggc tggtgccgta
      181 cagcatcggc cggccgatta cttccgctct gccgacgatt tttatcagct gtcgttccat
      241 gaggctgcgt aaaacttctc cgcatgctac gccgcggatt gattctatgt ccgcccgcag
      301 aatcggctga cggtaggcga ctatcgccag tgtttccaga gccgcctgac tgaggcggga
      361 ctccttgtga acgttcagca gctttttgag gacgtcgtta tattccggca gggtcatgag
      421 ctgataaccg cctgcgatgt tcatcacgcg gaaggaacag ccgccttttt gataacgttc
      481 gttgagagcc agtattgcct ttttcaacaa acggacgggt aattctgcga tagtggagag
      541 ttttgccggc ggaaccggac tgccggatga aaacagtatt gcctcgattg tgctggccat
      601 ctcatcaagg gcagcctgct gcacttcctg ttcgctttgc ggagtgagcg gcgcgttgtc
      661 tgcgtctgcc ggcgcagccg aaacatccga catctgttcc gctgtcgatt catccgaatt
      721 gcagacttct tcgtttttcg ccggcaagtc ggtctgttcg ttgctttctt cgctcatcaa
      781 tatctccatg ccgtccgtgg cagggtacgc taaaaaccca aatcgcaaat atcaaactgc
      841 ctctaggata ataaaataat aaaaaatctc aaggcgcaaa aaaacaacag ccgccttaga
      901 agcgtctgtt gttgtttaac atgctctaaa aagttccatc gtgattcaga tgatgcattt
      961 cttattagcg gtcgattgca tatcccaaaa aactcgtcaa atcggctttt tagtcccatt
     1021 caacttcata catactctcg acaggcgtcg ccgagccttt tatcggcata tcagccgtcg
     1081 ggacggcaac gtttttaagg gctttgggcc gatgagtcgc ttcggcaggg ttttctgcgg
     1141 gcactggaat ggcggcgata tcagggccgg cggcggttgt cttggcgatg gcggtttctt
     1201 ctgccattgg cgtttcaatc ggttttactg tttccgtcga ttcgttttcc gcagtttcat
     1261 tttcagatgg agcggttatc gacttcaacg ccggagccgt gggcagtttt tcttcatcta
     1321 ttttgacgac ttccgtcgta ttttcatcga gtgacggcgg agtaagctgt tttaaatccg
     1381 gcgtagacgg cgtttgggcg acagcggttt tcggggttat atctgttgga acagccggag
     1441 cttctatagg tttgacttcc ggcgttttta ctactaccgg cggaacggct atctctttag
     1501 tcgctggttt ttccgtttct gtatctatgg caaccggcgc atcaagactt tttacttccg
     1561 gcacggctgg aatagcttcc tgcgaagtct ttttgggttc gattgctact gttggcgaag
     1621 gctcaagcgt ttcgacttcc ggaattttgg gagactccac atttgcagcc tcagattcct
     1681 ttgtcgcggc gaccggattt ggcgcggcaa tcttgttgac ttcaactgga gccggtcctt
     1741 cttcttctgg tgttacggaa gcaatcggtt ttacgacagt tgacggctgt tcaacaggct
     1801 caactgttgc cggcgtcgga attgatggtt tttccgctac ttcaggagtt tttggagcag
     1861 gctgaggcag ggattccacc ggcggaagtt ccggtttttt tatggcaaca tctttcatgc
     1921 tttcggcagg cttggtttgt gcagccggcg tctgtgcctg tttggaagaa gcgggcagat
     1981 ctatatcttc gatggacggt tttgaaatgc ccttcgccat gcccaaatct tctggcgtta
     2041 gttggcctgc ttcattttca ttttgagctt ttgcagcagg aacggcaggc gtcttgactg
     2101 cgacattttc cgcagttgca ggttctgttt gctgttcctg ttgcggttcg gagatgtttg
     2161 tcgccggccg ggctgactcg ggctgttctt cgacggcgat ttttgctacc gcgtcttcag
     2221 ccgatgctgg tgaaatgtca gaaatcttta ttatctgtac gccggctttg cggtcgaaat
     2281 cctttctgcc gcttatgcgg acaacctggc cgatgtaggc attgacgtcg atagtcgaat
     2341 cggcaggccg ggcgaaagtg agtatctcgc ctgtgttggg attacgcaga atgtatatct
     2401 ggccgatagc cgaatcgtaa cggtcaaaaa ctcggctggg ctggattttg ccgatgacga
     2461 cattttctgc cggcgtctgc ggcttggcgg gttgcggctt taccggctca ttgacttccc
     2521 tgatggtttt tgagacgagt tcctgtgtac gacgggtttc gtcggcaatc ttgatttcgt
     2581 ccttgatgaa atcgaccatt gaatcgtaaa tcggcttaaa acggctgccg tcaggaacgt
     2641 tgatagcctg ggcttctgca agcagtttgt gcatgtcgcg ctgttccatc ggcttggcgt
     2701 tttcagccat cagatttttt tcaaggatgc gtatctgccg cagcatatcc gattcactgc
     2761 ggacgcctgg ttcaggtttt ttgtttacga gccgcacgtc aggtgcaacg gcggcagacc
     2821 cgccggttgc cgtcttttct tctatccgct tcggcgtcga tgaaaccgcc ggtgtttcag
     2881 gtgttacggc gacagtcgat tttgtttcgg cggactcagt cgtggcggct ggttcttcgt
     2941 actcgctttc cggcgcagtg tcagttgacg tttcgccgat tttctgaaca tattgcgatg
     3001 aaatgaagaa tctcgcctta gccggcggca atattatgta ccaggt
//
LOCUS       DAONFI010000298         2989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_41558_length_2989_cov_3.763122, whole genome shotgun sequence.
ACCESSION   DAONFI010000298 DAONFI010000000
VERSION     DAONFI010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41558_length_2989_cov_3.763122"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLK08_08870"
     CDS             complement(<1..923)
                     /locus_tag="PLK08_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53652.1"
                     /translation="MRSSISNSELRRPDMNYARLWLLAAVMTLAILGSCDVLIRMYGV
                     TPSLVDDMKLWEYTRDGMLTGTASANQVIFIGTSRANLGIDPGIFEKETGCDVLKLGI
                     AGSEAWPLLRDIAENTDFRGTIFYDFRADACSRNDIAQITEKYIREYYKNYSNLGKYD
                     KILNEKIAIFFQSSLAIVDIAPENLNRIFLPEKARADNYVVIGKDRATKAYYRTKLTA
                     KGIAKNRKKRIREAREACAGKCHDDSMWQCRIREIGKWVKLVQDRGGRVVLVRFPTSG
                     EHWELDSAAYPREKYWNKIAGLTGAVTIHFK"
     gene            complement(898..2310)
                     /locus_tag="PLK08_08875"
     CDS             complement(898..2310)
                     /locus_tag="PLK08_08875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPS53653.1"
                     /translation="MLFNSYSFFIFFAVVLIISRLNWPWRGKKVFLLVMSYVFYAAWN
                     PPFVVLLWISTVADWYFAGWMGKTGNRTKRKLLMFGSLFVNLGMLGFFKYGTFAADNF
                     TAMVQAFGFDFVPAKADIILPVGISFYTFQTLSYTIDVYRGQLKPWHSFLDYSLYVTF
                     FPQLVAGPIVRAAEFLPQCEEPKKATAGQLGWGLCLFIVGLFNKVVIADHFMAGTVEQ
                     IYGSTAHVGFFGAWAGTLAFSMQIFCDFAGYSTCAIGVAMCLGFVLPDNFRFPYSAIG
                     FSDFWRRWHISLSTWLRDYLYIPLGGNRHGAFMTYRNLFLTMLLGGLWHGASWTFVVW
                     GGLHGIYLVIERLLKTFVADIPLWHGRIFKAFFWFLTFLGVCFAWVFFRAQTFGSAWA
                     ICRSMAGITDDFSISGFSRVYAFVAVIVGLHIYMRNRSLEYLVSKVPVPVISLILAVM
                     IFLIMMCLNGDENAFIYFQF"
     gene            complement(2300..2752)
                     /locus_tag="PLK08_08880"
     CDS             complement(2300..2752)
                     /locus_tag="PLK08_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPS53654.1"
                     /translation="MKTLLVCIDFSDVTECLISNAIKLAQAFEEKIFLLHVTEPDPDF
                     IGFSTGSKTVREQWAKKFSREHAALCEMQKRLHSTSLEVEGIVVQGAVADKILQHVAS
                     LHADMILMGTHGHGGLYNLLVGSVTGNVLKRATCPVVLVNAKEEKNAI"
BASE COUNT          852 a          784 c          639 g          714 t
ORIGIN      
        1 tccttgaaat gaatagtgac cgctccggtc agaccggcaa tcttattcca atatttttca
       61 cgcggataag ctgctgaatc cagttcccaa tgctcgccgg atgtcgggaa acgcaccagt
      121 acgacgcggc cgcctctgtc ctgaacaagt ttcacccatt tgccgatttc cctgatgcgg
      181 cattgccaca tggaatcatc atgacatttt ccggcacaag cctcgcgggc ttcccgtatg
      241 cgtttcttac ggttttttgc aatgccttta gccgtcaatt tcgtacgata atacgccttc
      301 gtcgctctgt cttttccgat gacgacataa ttatcggctc ttgccttttc gggcaaaaat
      361 attctgttca gattttccgg agcaatatca actatggcaa ggctgctttg gaaaaaaatc
      421 gcaatttttt cattcagaat tttatcatat ttcccaaggt tggaataatt tttataatat
      481 tcgcgtatat atttttcggt tatttgtgct atatcattgc ggctgcaggc atcagccctg
      541 aaatcataaa aaatcgtgcc gcgaaaatcc gtattttcgg caatatccct gagcaacggc
      601 catgcttcag aaccagcaat gccaagcttt aaaacatcgc agccggtttc tttctcgaaa
      661 ataccaggat cgattcccag atttgcccgc gatgttccga taaatatgac ctgattcgcg
      721 gaagcagtac cggttaacat tccatctctc gtgtattccc acaatttcat gtcatccacg
      781 aggctgggag tcacgccgta catacggata agcacatcgc aacttccgag tatggccagc
      841 gtcattacgg cggcaagcag ccagagtctg gcataattca tatccggccg tcgcaactca
      901 gaattggaaa tagatgaacg cattctcatc tccattgagg cacatcatta tcaaaaaaat
      961 cataacggca agaatcaatg aaatgaccgg cacggggact ttcgatacca gatattccag
     1021 tgaccggttt cgcatgtaaa tatgtaagcc gactatgacg gcgacgaatg cgtatacccg
     1081 gctgaagccg gaaattgaaa aatcatccgt aattccagcc atgctcctgc agatagccca
     1141 cgcgctgcca aaagtctgag ccctgaaaaa cacccacgca aaacatacgc ccagaaaggt
     1201 aagaaaccag aaaaaagcct taaaaattct gccgtgccat aatggtatat cagccacaaa
     1261 cgtcttcaac agccgctcaa tcacaagata aattccatgc aagccgcccc atacgacaaa
     1321 cgtccagctt gcgccatgcc acaatcctcc cagcagcatc gtaaggaaca gatttctgta
     1381 agtcatgaac gctccgtgcc tgttgccgcc caacggaata tacagatagt cccgcaacca
     1441 cgttgaaaga gaaatatgcc accttctcca aaaatcggaa aaaccaatcg cgctatacgg
     1501 aaagcggaaa ttgtccggca gaacaaaacc caggcacatc gccacgccta ttgcacatgt
     1561 ggaatagcct gcgaaatcac agaatatctg catgctgaac gccaacgtcc ctgcccatgc
     1621 gccgaaaaat ccgacatgag ccgtcgagcc ataaatctgt tccaccgtgc cagccatgaa
     1681 atggtcggca atgacaacct tgttaaaaag gccgacgata aagagacaca atccccagcc
     1741 gagttggccg gctgttgcct ttttgggttc ttcgcattga ggcagaaatt ccgccgcgcg
     1801 cacgataggc ccagccacca actgggggaa aaatgtaaca tacagcgaat aatcgagaaa
     1861 actgtgccac ggcttgagct ggccgcgata tacgtcgata gtgtaactca atgtctggaa
     1921 cgtgtaaaaa cttatgccga cggggagaat tatatcggct ttggccggga cgaaatcaaa
     1981 gccgaacgcc tgcaccatcg ccgtgaaatt atccgccgca aacgtgccgt acttgaaaaa
     2041 gccgagcatg ccgagattca caaataaact gccgaacata agcagtttac gtttcgtacg
     2101 atttcccgtt ttgcccatcc aaccggcgaa ataccagtcg gcgacggtgg aaatccacag
     2161 cagaacgaca aacggcggat tccaggcagc ataaaaaaca taactcataa caagcaggaa
     2221 gacttttttt ccccgccacg gccagttcag cctgcttatt atgaggacta cggcgaaaaa
     2281 tatgaaaaaa gaataagaat taaatagcat tcttttcctc ttttgcgtta acaagaacta
     2341 cggggcaggt tgcacgtttc aaaacattcc ctgtaacgct gccgaccagc aaattataaa
     2401 gtccgccatg gccgtgagta cccatcagaa tcatatcagc atgaaggctg gcgacatgct
     2461 gaagaatctt gtccgccacg gcgccctgaa cgactatgcc ctcgacttca aggctggtcg
     2521 agtgcaatct tttctgcatt tcgcaaagtg ccgcgtgttc tcgggaaaat tttttcgccc
     2581 attgctccct tacggtcttt gagccggtgg aaaacccgat aaaatcagga tccggctcgg
     2641 taacatgcag aagaaatatc ttttcttcaa acgcttgggc aagttttatg gcattagaaa
     2701 tcagacattc agtaacgtct gaaaaatcta tgcaaaccag cagtgttttc atcggccaca
     2761 tctccttttt cggcagagtc tatcaaatcg aatcttttgt ttcaccctta ttaattttaa
     2821 aaagatgaaa aatttcaata cagggcttga cacagccgga attaatttta ttattgttac
     2881 tccctgagtg cctttttcat tcgcacgggg cgccccgaca ggaaggtcgg gagtggacag
     2941 gtcgaggttg cggccgcctg ttcaggcctc cgaatgaagc ccccgccgc
//
LOCUS       DAONFI010000299         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_41886_length_2973_cov_4.473955, whole genome shotgun sequence.
ACCESSION   DAONFI010000299 DAONFI010000000
VERSION     DAONFI010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41886_length_2973_cov_4.473955"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLK08_08885"
     CDS             complement(<1..906)
                     /locus_tag="PLK08_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPS53655.1"
                     /translation="MIDAVMEMVHKRNNAETEFHQAVREVMESIEPVIKRHPEFVKAR
                     IIERLIEPERQVIFRVPWVDDKGEIHVNRGFRVEFNSAIGPYKGGLRFHPSVYLGIIK
                     FLGFEQIFKNALTTTPIGGGKGGSDFDPKGKSDMEVMRFCQSFMSELFRHIGPDTDVP
                     AGDIGVGAREIGYLFGQYKKLTNSFSGVLTGKGLSYGGSLVRTEATGYGLAYFVTHML
                     TSRGTNWDGKNVVVSGSGNVAIYATQKVHQLGGKVVAMSDSSGYIYDPQGIRLGTVKR
                     IKEIERGRIRQYAAEHNSAQYFEDCQ"
     gene            1226..2239
                     /locus_tag="PLK08_08890"
     CDS             1226..2239
                     /locus_tag="PLK08_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HPS53656.1"
                     /translation="MDKKNLKKIAILNVLKEQAKPVSSARITQLLNASGYDFSERTVR
                     FYLKELDRDGLTVSCGRKGRNITEQGLSEFHSSQIMHRTGYLSARIDQMMYRMDFDLP
                     RRSGKVVVNTSVADPQMLLGYADEICQVFDKGYAMGKLLTLLSPGETIGETVIPDGKV
                     GFCTVCSITVNGVLLKHGVPVSSRFGGLLELVGGKATRFVEMIHYDGTSIDPLEVFIR
                     SGMTDYHGAIRDGNGQIGGSFRDIPADSRELVSHISERLDSIGLGAIMELGLPGQPLL
                     DIPVNYGQIGAIVVGGLNPIAILEEQECRVVSRALNGLMDYSRLFYFDEFPLRLKKII
                     AES"
     gene            complement(2598..>2973)
                     /locus_tag="PLK08_08895"
     CDS             complement(2598..>2973)
                     /locus_tag="PLK08_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53657.1"
                     /translation="GQSLGSGADRLDVIWAEPTFWNLYGTNYIAAGWWESVCAGVIGI
                     WVNLLLGVLAAYLITYLASSSTAIHFILRNECESTNYDCIYMENEDDLPLDVKPAEKP
                     QEETSTPETPEEPEEEPMPDGC"
BASE COUNT          686 a          767 c          773 g          747 t
ORIGIN      
        1 ctggcagtct tcgaaatact gtgccgaatt gtgttcggcg gcgtactggc gtatacggcc
       61 gcgttcgatt tccttgatgc gcttgaccgt gccgagccgt atgccctgcg ggtcgtagat
      121 gtatccgctc gaatcgctca tggccacgac cttgccgccg agctgatgta ccttctgcgt
      181 ggcataaatg gctacattgc ccgagccgga caccactaca ttcttgccgt cccagttcgt
      241 gccgcgactc gtgagcatgt gcgtcacgaa atacgccaag ccgtaccccg tagcctccgt
      301 acgaaccagc gaaccgccgt aggataatcc cttgcccgtc aatacgccgg aaaaagagtt
      361 ggtcagcttt ttatattggc cgaacaggta gccgatttct cttgcgccta cgccgatatc
      421 gcccgccggc acgtcggtat cagggccgat gtgacggaac aactcgctca tgaagctctg
      481 gcagaatcgc atcacttcca tatcgctctt gcctttgggg tcgaaatcac tgccgccctt
      541 gccgccgcct atgggcgtcg tggtcagagc atttttgaat atctgttcga aacccaggaa
      601 ttttattatg cccaggtaga ccgacggatg aaaacgcagc ccgcccttat atgggcctat
      661 cgcactgttg aactcaacgc ggaagccgcg gttcacatga atctcgccct tgtcgtccac
      721 ccacggcaca cggaatatta cctgccgctc aggctcgatg agccgttcga ttatcctggc
      781 tttgacgaat tcaggatgac gttttatgac cggctcgatg gattccatca cctcgcgaac
      841 cgcctgatga aattccgtct ccgcattgtt ccgcttgtgg accatttcca ttactgcgtc
      901 tatcatgcat gtttcctttc gcttataaag attcatatcg gaagccgtcg gcgtcggatt
      961 aacggtaata ttttttcatg tttacggtaa tatattgccg ttaatcaagc cgcaaaagaa
     1021 taaatatcac acttattatc aatgtcaata gggataatct gctgaggtga tattatggtt
     1081 atcggcaaaa ttgccgcaac tgccgttttt cggtaaaaag ttttccttat tgccgaaaag
     1141 ttattataat aacctgaaat agctgttgca gatggcggga caacggctat tttgccgaaa
     1201 cggcttggta taaggcgaat taaccatgga taaaaaaaat ttaaaaaaaa ttgcgatact
     1261 caacgttttg aaagaacagg ccaagcctgt gagctcagcg agaataacgc aactcctcaa
     1321 cgccagcggt tacgacttca gtgagcggac ggtgcggttt tatctgaagg aactggaccg
     1381 cgacggcttg accgtgtcgt gcggccgtaa gggccgcaac attaccgagc aggggctgag
     1441 cgaatttcac tcgtcgcaga taatgcatcg caccggttat ctgtcggcaa gaatcgacca
     1501 gatgatgtat cgcatggatt tcgatttacc gcgtcgctcc ggtaaagttg tcgttaacac
     1561 gtcagtggct gacccgcaga tgcttttggg ttacgccgac gaaatctgcc aggtcttcga
     1621 caagggttat gccatgggca agctgcttac gctgctgagt cctggagaga caatcggcga
     1681 aacagtaatt cctgacggca aggtgggatt ctgcacggtc tgttcgataa ccgtgaacgg
     1741 cgtattgctc aaacacggcg taccggtgtc gtcgcggttc ggcggcctgc tggaactggt
     1801 cggcggcaaa gcgacgcgat tcgtggagat gatacattac gacggaacga gcatcgaccc
     1861 gctggaggtt ttcatacgca gcggcatgac tgattatcac ggggcgataa gggacggcaa
     1921 cggccaaatc ggcggaagtt tccgcgacat accggctgac agccgcgaac tggtttcgca
     1981 cataagcgaa cgacttgatt cgataggcct cggcgcgata atggaactcg gcctgcctgg
     2041 tcagccgttg cttgatatac ccgtaaatta cggccaaata ggcgccatag tagtcggcgg
     2101 cctgaatccg atagcgattc tggaagaaca ggaatgtcgc gtcgtatcac gcgcacttaa
     2161 cggcctgatg gattacagtc gcctgttcta ttttgacgaa tttccgttgc ggctgaaaaa
     2221 aatcatcgct gaaagttaga gggtgttctc aattaagctg tgagacgcaa atgagtggca
     2281 attgtgcaga tgcaaggaac aaaaacgatt gcgaatttgt agattcgctg agtttttgtg
     2341 acgccgcaga tgcacgattg ccgctcacaa cctttaatat attatttttt gcggccgcag
     2401 ggtaattgag aacgccctct agagcatttt aaacttgata tgcgtcattt ctgcttgctg
     2461 caacatcgcg ctgctgcatc aggctgcatc ggaatacggg cagtattcct gctttgcctt
     2521 ccttgcttgg ctcgtttcgc caacgcataa agtgtcgcat ctcaagttta aaatgctcta
     2581 tattttccgg acgcatatca gcatccgtcg ggcataggtt cttcttccgg ttcttcggga
     2641 gtttcgggcg tagatgtttc ttcctgcggt ttttcggcgg gtttgacgtc gagcggcagg
     2701 tcgtcttcgt tttccatgta gatgcagtcg tagttggtcg attcgcactc gttacggagt
     2761 atgaaatgga tagccgtcga actgcttgcc agatacgtaa taaggtacgc cgccagcact
     2821 cccagaagca gattaaccca tatgccgatg acgccggcac aaacggactc ccaccagccg
     2881 gctgctatgt aattcgtgcc gtacagattc cagaacgtcg gctcagccca tatcacgtcc
     2941 agccggtctg cgccgctgcc cagcgactgg ccg
//
LOCUS       DAONFI010000300         2953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_42299_length_2953_cov_3.835749, whole genome shotgun sequence.
ACCESSION   DAONFI010000300 DAONFI010000000
VERSION     DAONFI010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42299_length_2953_cov_3.835749"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1757)
                     /locus_tag="PLK08_08900"
     CDS             complement(219..1757)
                     /locus_tag="PLK08_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53658.1"
                     /translation="MNIEREKIRTILNEYLDGELTPQQADEVAAALAADSQLAAEYAE
                     LQATRATIGSLGSITAPPDMTERIMHATEHHIFRDAETPRRSGTFEKIFHVQVFAAAA
                     VVLLAVGMGLYLINTLSKSQEMKPAVAIGKASPTENSAKQIFDQEKLKRDEATAYEKA
                     ISPKMVKVVPVCTQKLKSTSVAAAKGISDSRIAGPGSQTKITIAALEDKIRTKESDSR
                     TAVADIKPVIEPEPSLAMNDYKSRENYDSAWEQHAKKTEEASPTLPQPAITPPVLKTP
                     RPVGEIAAAGTPKKVNGMSFYKGDASGKAKTISSRAALAKSVAIGKTTQDMPDSRHTQ
                     NIIIETTNVGQTQMKLENYMKSNRIQIVSSQKFHEAAVVDARKSELNQNIQSLATWAD
                     KEPVKLEYHIKTSETQACQINADLAQNFPNAQNILPSIKNSGLAVRENDGERMRGKLA
                     ESNLADSSVTNAIDANPIMMTITIQQRSQNQAIPTATCKPASVTTSAPATQPLSQPTT
                     APAK"
     gene            complement(1754..2353)
                     /locus_tag="PLK08_08905"
     CDS             complement(1754..2353)
                     /locus_tag="PLK08_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPS53659.1"
                     /translation="MEKHKASHDFADEAAIIRRCCEGESAAFEVLVRRYQDRIFRMAF
                     GLLGSYADAEEVTQEVFLKAFEKLGTFRQKSSFYTWLFRIATNEALTHRRQKKRVSFF
                     SIFAGKDDDPPGDLPNKKQPQPDRPMIENENIIAVRNALSKLDMDFRTVVVMRDMENM
                     NYNEISQVLGIPVGTVKSRISRARLMLRNLLVDVVRNDS"
     gene            2654..>2953
                     /gene="ssrA"
                     /locus_tag="PLK08_08910"
     tmRNA           2654..>2953
                     /gene="ssrA"
                     /locus_tag="PLK08_08910"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          660 a          680 c          745 g          868 t
ORIGIN      
        1 catgaggtgc cagcgataca ggctccactt gggcgggcca tccttgccgg catgcgtcac
       61 gccgaatgtt tttaaagtgc ttacggaaaa attcaatatc atgcccatat ccacgccgca
      121 aaaagacatc agggcaatac ttggatagag aaggcctcaa tgaaataaag cagggcggca
      181 gggacgttgg ctcaccttgc gtattttcag aaattcagtt attttgccgg tgcggtagtc
      241 ggttgagaca gcggctgagt tgcaggtgcg gaggttgtca cggacgcagg cttgcaagtg
      301 gctgtaggaa tagcttggtt ttggctacgc tgctgaattg taattgtcat cattatcgga
      361 ttggcatcta ttgcattggt aacggatgaa tcggctaagt tgctttcagc caatttcccc
      421 ctcatgcgtt cgccgtcatt ttcccgaact gccagtcctg aattcttaat agacgggagg
      481 atgttctgtg cgtttgggaa attttgggca aggtcggcat taatctggca ggcctgcgtt
      541 tccgatgtct ttatatggta ttccaatttt acaggctctt tatcagccca tgtcgccagc
      601 gattgaatat tctgattcaa ttcgctttta cgtgcatcaa ctacggcggc ttcatgaaat
      661 ttttgcgacg aaacaatctg aattctgttt gatttcatat agttttcgag cttcatctgc
      721 gtctgaccga cgttggttgt ttcgatgata atgttttggg tgtgtcggct gtcgggcata
      781 tcctgagtcg ttttaccgat agcgacagat ttggctaacg cagcgcgaga cgatatcgtc
      841 tttgccttac ctgacgcgtc gcctttataa aaactcatgc cgtttacttt tttcggcgtt
      901 ccggcggcag ctatttcgcc gaccggtctg ggggttttca atacaggggg tgttattgca
      961 ggttgaggaa gggttgggga agcttcttca gttttttttg catgttgctc ccatgcagag
     1021 tcgtaatttt cccgagactt gtaatcgttc atcgcgagtg acggctcagg ttcgataaca
     1081 ggttttatat ccgctacggc agtcctggag tctgattcct tggttcggat tttatcttca
     1141 agagcagcta tggttatttt tgtttgcgaa ccagggccgg cgattctgga atcggagatg
     1201 cccttagccg cggcgacgga tgtcgatttc aatttttgag tacaaacagg aacgactttt
     1261 accattttcg gagagatagc tttttcgtag gctgttgctt cgtcgcgttt taatttttct
     1321 tggtcaaaga tttgtttcgc cgaattttcc gtcggggagg ccttgccgat agctactgct
     1381 ggtttcattt cctgtgattt tgaaagcgta tttattagat acagtcccat gcctacagcc
     1441 agcagcacaa cggcagccgc ggcaaaaacc tgcacgtgaa aaattttctc gaatgtgccg
     1501 ctacgccggg gtgtttctgc atcgcgaaag atatgatgtt ccgttgcgtg catgatacgt
     1561 tccgtcatat ccggcggagc ggtaatgctg ccaagagaac ctatagtcgc tctggtggct
     1621 tgcaattcgg catattcggc ggctaactgc gaatccgcgg cgagagcggc agcgacctcg
     1681 tcagcctgct gcggcgtcag ttctccatcg agatattcgt taagaatcgt cctgattttt
     1741 tcacgttcaa tattcatgag tcattccgca caacgtcaac aagcaggttt cgcaacatca
     1801 gccgcgcacg gctgatgcgg ctcttgacag tacccacagg tatgcccaaa acctgcgata
     1861 tttcattata gttcatgttt tccatgtcgc gcattactac gactgtccga aaatccatat
     1921 caagcttcga caaggcattt ctgactgcga taatattttc attttcaatc atcggcctgt
     1981 caggctgcgg ctgtttttta ttgggcaaat cgcctggcgg gtcgtcgtct ttgccggcga
     2041 atatcgaaaa aaaactgaca cgttttttct ggcgtcggtg cgtaagagcc tcatttgtcg
     2101 caatcctgaa cagccatgta taaaaactgc ttttttgcct gaatgtgccg agtttttcaa
     2161 acgccttcag gaatacttcc tgcgttacct cttcggcgtc ggcataactg ccgagcagtc
     2221 caaacgccat gcggaaaatt ctgtcctggt atcgcctgac cagaacttca aacgccgcag
     2281 actctccttc acagcatcgc cggattatgg cggcttcgtc ggcgaagtcg tgggaagctt
     2341 tgtgcttttc caacggctgt tctttaatat ttgactgcgt ttcgctcaaa aagttcccca
     2401 aaatgataga aaggacaact ttttatgaca atatctcgtt tattcagccg acataaacgc
     2461 acgacaagcg tttgattaat gccggccgtt ttgtaaaaca tctaacaggt ttattttaat
     2521 gactctctgc cgaagataca atataaacat gctaatctgt atggtgctta aaaatgatga
     2581 tatgcctgga taatgatgtg gtactgccgt ggaaatatag tataataagg agttgaaata
     2641 cttcgatgaa ttcgggggcg aattggattc gaccggatgg gttgaggtat cggttgcgtg
     2701 tcgaggttcc aggtggcctc gttaatccct ggaaaacttt agttgccaaa ccgcaactta
     2761 gaatggccgc ttaattaagc tggcccccgt ttcagctgcc gcctgagcgg ctgattccgg
     2821 tgaaacattc gggcttaccg cagagagtgg ccgggttact ttgcgggaca tttatccggc
     2881 tggctctgac ggatgtttgc cggcctgctg ccgcggagcg agattcaact gaccggctac
     2941 acacgtagat gcc
//
LOCUS       DAONFI010000301         2928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_42810_length_2928_cov_4.493213, whole genome shotgun sequence.
ACCESSION   DAONFI010000301 DAONFI010000000
VERSION     DAONFI010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42810_length_2928_cov_4.493213"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PLK08_08915"
     CDS             complement(<1..175)
                     /locus_tag="PLK08_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPS53660.1"
                     /translation="MQGSRLILASASPQRKKLMKEAGYEFDVCVSPLDEPNHMARQMH
                     PASHSEALAWFKCA"
     gene            complement(375..854)
                     /gene="smpB"
                     /locus_tag="PLK08_08920"
     CDS             complement(375..854)
                     /gene="smpB"
                     /locus_tag="PLK08_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005528259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPS53661.1"
                     /translation="MAKGKKQPEHHGVRIGNRRAFHDYHIHEKLECGIELLGTEVKSL
                     RAGQAKIDDAYARITTAGEVYLVGANIAQYPQAAEGMQHDPTRKRRLLLHRRQIEKLE
                     VHVKQKGKTIIPLAIYFKKGWAKCELGVAEGKRDYDKREAIRTRQQQMDIKRELKRY"
     gene            complement(893..2275)
                     /locus_tag="PLK08_08925"
     CDS             complement(893..2275)
                     /locus_tag="PLK08_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPS53662.1"
                     /translation="MENGQNNSARDLAVKALRDRGGNVSAHLSRVADEIGLSSADRAF
                     AHELAIGTLRRRATLDAILKSYLNMPGRKLNPPLQEIMQVALYQLLFLDRVPAFAAVN
                     EAVRQAHAFRQKKQAGLINGVLRNIIREMSAPSEHALSPQPAANIIPISPTKDRRMER
                     NVFPEPERNRALWLSAAYSLPAELAADWVGQFGWYEQVTAIAIHSCSRPPLIARVNSL
                     KAKNADVLARLVAAGMDAKLHENGLSIVLDSVEGMNSLAEFQEGLLQPQDPTATSVVA
                     AAKIRPGMRVLDFCASPGTKTTHIAERMENSGLIIAADVSGEKLKRIEENALRMGINI
                     IKTIRAEDVGGLEPRSFDLVLADVPCSNSGVLARRVEARWRYSKADISKLVRDQQFLA
                     QAAAQFVRPGGQLIYSTCSINPDENSRVVKFLAKNVSHLKLKFEKLTLPAGADDITRW
                     HDGGYMAIFE"
     gene            complement(2579..>2928)
                     /locus_tag="PLK08_08930"
     CDS             complement(2579..>2928)
                     /locus_tag="PLK08_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPS53663.1"
                     /translation="TATMSSTSAHFYLIMSNFYTDSSMVVNVNGSSQTFTTIFGSTSG
                     SVSKLFDISIADVDAGDTLTVTLNNYTVNSSWGNLVPWAAAMTTVVPEPLTAGLLAVG
                     GAFIFIRRRRNRF"
BASE COUNT          737 a          734 c          685 g          772 t
ORIGIN      
        1 cggcgcattt gaaccacgcc aacgcctccg aatgagacgc aggatgcatc tgtctggcca
       61 tatgattcgg ctcatcaagc ggtgaaacgc agacgtcgaa ttcatacccc gcctctttca
      121 tgagtttttt acgctgcggc gacgcagagg ctaatattaa tcgtgaacct tgcaaatttt
      181 cagcccggaa tgaattttta agtgttaaaa cggttttcta aaaaattttt attttgaaaa
      241 tgtagaattt acgaatgatt tatttaaaaa atttacagtt ttaaaagcgt aaaattacaa
      301 atgacatatt taaaaaattt gcggttttaa aaacgtggaa ttacgaatga catatttaaa
      361 aaattcgtaa aatatcagta acgtttgagc tcgcgtttga tatccatctg ctgctgccgc
      421 gtacgaatag cctcgcgttt gtcatagtcg cgtttgccct ccgcaactcc caactcacac
      481 ttggcccagc cttttttgaa ataaatcgca agcggaataa tggtctttcc cttctgcttg
      541 acgtgaactt cgagtttttc tatctggcgg cgatgcagaa gcaggcggcg ttttcgcgta
      601 gggtcgtgct gcatgccttc ggcggcttgg gggtactggg cgatgtttgc cccgacgagg
      661 tagacttcgc cagccgtcgt tatgcgggca taagcgtcat ctatcttggc ttggccggca
      721 cgcaacgact tcacctccgt tccgagcagc tcgataccgc attccaattt ttcatggatg
      781 tgataatcat gaaacgcccg acggttgccg attcgtacgc cgtgatgctc aggttgtttt
      841 ttgcctttcg ccatttaaaa ttcactgtat ccagctatcc gcaatgaaaa aatcattcaa
      901 aaatcgccat gtaaccgccg tcgtgccatc tggtaatatc gtctgcgcca gccggcagtg
      961 tgagtttctc aaatttcagc ttcagatgcg aaacattttt cgccagaaat ttcacgaccc
     1021 gcgaattttc atcggggttg atgctgcaag tgctgtaaat cagctggccg cctggacgta
     1081 cgaactgtgc ggcagcctgg gccagaaatt gctggtcgcg tacgagtttt gagatatcgg
     1141 ctttgctgta gcgccaacga gcctccaccc gtcttgccag cacgccggag ttactgcacg
     1201 gcacatcagc caacacaagg tcgaaactcc gcggctcaag tccgccaaca tcttcggctc
     1261 ggatggtttt tataatattg attcccatcc gcagggcatt ttcttctatg cgtttaagtt
     1321 tttcgcctga aacgtcagcg gcaattatca ggccggaatt ctccatccgt tcggcaatat
     1381 gcgtggtctt cgtgccaggc gatgcgcaga aatccagcac ccgcatgccg ggacgaattt
     1441 tggccgccgc cacaacagac gtagccgtcg gatcttgcgg ctgaagcaga ccttcctgaa
     1501 actcagccaa cgaattcata ccctcaacgg aatccagaac gatactcaaa ccattctcat
     1561 gcaacttcgc atccatgccg gcggctacca gcctggctaa cacatcggcg ttttttgctt
     1621 ttaacgaatt tacgcgagca atcagcggcg gccgtgagca ggaatgtatc gctatcgccg
     1681 tcacctgttc ataccagccg aattgtccga cccaatcggc ggcaagctcc gccggcaggg
     1741 aataagccgc cgacagccat aacgcccgat ttctttccgg ctcaggaaag acgtttcgtt
     1801 ccattcgacg gtctttggtg ggcgatatcg gtattatgtt cgcagccggc tgaggtgaaa
     1861 gagcgtgttc agacggagcg gacatttcgc ggataatgtt acgcaaaacg ccgttaatca
     1921 ggccagcctg ttttttctgt ctgaacgcat gagcctgccg gacggcttca ttgacggcgg
     1981 caaacgccgg cacacggtca agaaacagaa gctgataaag cgcaacctgc atgatttcct
     2041 gcaacggcgg attgagctta cggcccggca tattcaggta cgatttcaga atcgcgtcca
     2101 gagtagccct ccgtcgtaat gtgccgatgg caagctcatg tgcaaatgcc ctgtcggcag
     2161 aactcaggcc gatttcatct gccacccgcg aaagatgtgc cgatacattc ccgccgcggt
     2221 cacgcaatgc cttcaccgcc aggtcgcgag ccgaattgtt ttgtccgttt tccatagttg
     2281 ttactttctt ttgcgggtaa tattagtttt atcgcaaaag cattgcaagg ctcatagtta
     2341 cattttgtgt ttccaaaaaa tcaaaagcaa agcgatacgg gctaaagcct gcgtttccat
     2401 atggttatga gttttggggt gtcactgaat gacgagtgct ttccatcagc tatcggttaa
     2461 aatcgcaaaa ttaagaatga catattaaaa aaccccggac gttttgccgt cggggctttt
     2521 tttaaaacaa acacaaattt taatgaaagc tattttgaaa aagtttctat ttattaatct
     2581 agaaacggtt tcttcttcga cgaataaata taaatgctcc accaacagct aacagacctg
     2641 ctgttagagg ttcaggaaca accgtagtca tggctgccgc ccacgggaca aggttacccc
     2701 aacttgagtt aaccgtgtaa ttgttgagag tcaccgtcaa tgtatctccg gcatccacgt
     2761 ctgcaatcga tatgtcaaag agcttcgaaa ccgaccctga cgttgaaccg aatatggtag
     2821 tgaatgtctg tgaacttcca ttgacgttaa cgaccatgct ggagtcagta taaaaattcg
     2881 acattatcag atagaaatgt gccgaggtcg acgacatggt ggctgtga
//
LOCUS       DAONFI010000302         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_42899_length_2924_cov_3.544789, whole genome shotgun sequence.
ACCESSION   DAONFI010000302 DAONFI010000000
VERSION     DAONFI010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42899_length_2924_cov_3.544789"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLK08_08935"
     CDS             <1..462
                     /locus_tag="PLK08_08935"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPS53664.1"
                     /translation="SVGEGHAAMCDVLDDIDALLPHDKPRYLMGVGEPRDIIAAVLRG
                     IDMFDCVMPTRNGRNAQAFTWNGKLRLRNAQYAEDTAPLDENCACYTCRTFSRGTIRH
                     LFMAREMLGPILLTLHNLHFFADFMRAIRESITAGTLETDSLKWLAAIYGK"
     gene            607..966
                     /locus_tag="PLK08_08940"
     CDS             607..966
                     /locus_tag="PLK08_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPS53665.1"
                     /translation="MEIDVEKRNGYVVITPICGQIDAGNYDELRKQIGSVLKTDHNIL
                     LNLENVQFLDSSAASVILYCYRNIQLHQGNFEICAPSIAVNVTLKLLKMDRLTKVFHS
                     LEEAGRAFEDKINATQK"
     gene            979..1569
                     /locus_tag="PLK08_08945"
     CDS             979..1569
                     /locus_tag="PLK08_08945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPS53666.1"
                     /translation="MKSDLIKRTLDFIIGTVCAVFFLPIFLICVAIVKFSSPGPVIYC
                     QTRVGRYGRPFKMYKLRTMRHNAESQTGAVWACNNDPRIIPACRWMRLSHMDELPQLI
                     NVIRGDMSIVGPRPERLEILLNIDMEDRSKVIRRLDVKPGITGLAQIRSGYTVKRSSF
                     LRKLNYDLIYIKHYSPLFDLWIIFKTFPKLHDNKAN"
     gene            1702..1800
                     /gene="ffs"
                     /locus_tag="PLK08_08950"
     ncRNA           1702..1800
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLK08_08950"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2017..>2924
                     /gene="dnaX"
                     /locus_tag="PLK08_08955"
     CDS             2017..>2924
                     /gene="dnaX"
                     /locus_tag="PLK08_08955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPS53667.1"
                     /translation="MSEYLVLARKYRSETFEEVVGQEPICQTLINAIQTGRVAHAYLF
                     MGTRGVGKTTVARILAKALNCMNADGPTATPCNKCEACKSISRGEDVDVVEIDGASNR
                     GIDEIRELRANAVFRPARCRYKIYYIDEVHMLTKEAFNALLKTLEEPPSHVKFIFATT
                     EVDKVPATILSRCQRFDFRNIPTSKIAEHLAAICKTEKVPADSDALYRIARAAAGSMR
                     DSLSLLDQLLAGADKVTDDEVIRILGTPPDERVMAIVTAVADGNSAAALNELAAILDG
                     GITFASLLGAMGDVYRNMMLAVTCGPT"
BASE COUNT          761 a          771 c          735 g          657 t
ORIGIN      
        1 tcggtcggcg aaggccatgc ggcgatgtgc gacgtgctgg acgacatcga cgccctgctg
       61 ccgcacgaca agccgcgata tcttatgggc gtcggcgagc cgcgggatat catcgccgcg
      121 gtactgcgcg gcatagacat gttcgactgc gtcatgccga cccgcaatgg ccgcaatgcc
      181 caggcgttta catggaacgg caaactgcgg ctgcggaacg cacaatatgc cgaagatacc
      241 gccccgctgg acgaaaactg tgcctgttat acatgcagaa ctttttcccg tggtacgata
      301 cgtcaccttt tcatggcacg ggagatgctc ggcccgattc tgctgacgct gcacaacctg
      361 catttttttg cagacttcat gcgggcgatt cgggagtcaa taaccgccgg cacgcttgaa
      421 accgattccc taaaatggct agccgccatt tacggaaaat aaaccagccg cctttaccat
      481 ccgccaccga gtgtcaccct accgattttg aagcggctgc cgcttttaaa atatattgtg
      541 caactttcgc gtattccgct tgctagtgcg atttttggca tctataatgt tctgcaaggg
      601 agacaaatgg agatagacgt cgaaaaacgg aacggttatg ttgtaataac ccctatatgc
      661 ggacagattg acgcagggaa ctacgatgag ctgcgcaaac agataggttc tgtgctcaag
      721 actgaccaca atatacttct gaacctggaa aacgtccaat ttctggacag ttcagccgcc
      781 agcgtgattc tctactgcta tcgcaatatc caactgcatc agggcaattt cgaaatctgc
      841 gctccgtcga ttgccgtcaa cgtcacgctg aagctgctga aaatggacag gcttacaaaa
      901 gtgtttcatt ccctcgaaga agccggcagg gcatttgagg ataaaataaa cgctacacaa
      961 aagtgatttt atcgttaagt gaaaagtgac cttatcaaaa gaacgttgga ttttatcatc
     1021 ggcactgtct gcgccgtttt tttcctgccg atatttctca tatgcgtcgc aatcgtgaaa
     1081 ttttccagcc ctggccccgt tatttactgt cagacccgcg tcggcagata tggccggcca
     1141 ttcaagatgt acaaattgcg taccatgcga cataatgccg aatcgcagac cggcgcagta
     1201 tgggcatgca acaacgaccc gcgaatcatt cctgcgtgca ggtggatgcg tctgagtcac
     1261 atggacgaac tgccgcagct catcaacgta atcagaggtg atatgtccat cgtcggccca
     1321 cggccggagc ggctggaaat tctgctgaac atcgatatgg aagaccgctc taaggtcatc
     1381 aggcggctgg acgtcaaacc aggcataacc ggcctggcac agatacgcag cggttatacc
     1441 gtaaaacgca gcagtttttt acgcaaattg aattacgacc taatctatat aaaacattat
     1501 tcgccgctgt tcgatttgtg gataatattc aaaacatttc ccaaattgca cgataacaag
     1561 gcaaattgac gtcatcagcc gctatcgtcc gcgattctca acccgcccat cgccaacgtt
     1621 caaagaaagc cttgtttttt gaagcaaact ttcgtaaact catcagcccg ttgagttgcg
     1681 gaagcgactc tccgactggc ggcgtcgggg ccgggcaatg ccggcccgcg aacctggtca
     1741 ggtccggaag gaagcagcca taagcgataa gtctgcgtgt gtccgatttc ctggcgtcgc
     1801 cagtcggcga gtcgaccgca ttaaacgttc catgtgtaac tgtaatttag ggcatatttt
     1861 taaaaaaatg tcagattatc gataagatta cggaagacat gttaaaaaat tacgaaagac
     1921 atattaaaaa attgccaaag ttatcgataa gattacgaaa gacatattaa aaaattgcta
     1981 aagttatcga taagattacg aaagacatat taaaaaatgt cggaatacct tgtattagcc
     2041 cgaaaatacc gcagcgaaac ttttgaagaa gtggttggcc aggagcccat ctgccagacg
     2101 ctgataaatg ctattcagac cggcagggtt gcacacgcct acctgttcat gggtacgcgc
     2161 ggcgtaggca agactacggt agcccgaata ctcgccaaag ctcttaattg catgaatgcc
     2221 gacggcccta cggctacgcc gtgcaacaag tgcgaagcct gcaaatccat cagccgcggt
     2281 gaagacgtgg acgtggtgga aatcgacggt gcatccaatc gcggcataga tgaaattcgc
     2341 gaactccgtg ccaacgcggt tttccgtcca gcccgctgcc gctataaaat atactatatc
     2401 gacgaagttc acatgttgac gaaggaagcg ttcaacgccc tgctcaaaac gctggaagaa
     2461 ccgccttcgc acgtcaaatt catcttcgcc actacagaag tcgacaaagt gcccgccacg
     2521 attctctcgc ggtgtcagcg gttcgatttc cgcaatattc cgacgtcgaa aatcgccgag
     2581 catctggcgg caatctgcaa gacggaaaaa gttccagccg actccgatgc gctttaccgg
     2641 attgcacggg cggctgcggg ctcgatgcgc gattcgctca gcctgcttga ccagctcctc
     2701 gccggcgcag acaaggtgac cgacgatgaa gtcatccgaa tactcggtac gccgccggat
     2761 gagcgggtga tggctatcgt gaccgccgtc gccgatggca acagcgcggc agccttgaac
     2821 gaattagcgg cgatactcga cggcggcatt acgttcgcca gccttcttgg tgcgatgggc
     2881 gacgtttatc gcaacatgat gctggctgtc acctgcgggc cgac
//
LOCUS       DAONFI010000303         2921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_42961_length_2921_cov_3.873343, whole genome shotgun sequence.
ACCESSION   DAONFI010000303 DAONFI010000000
VERSION     DAONFI010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42961_length_2921_cov_3.873343"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLK08_08960"
     CDS             complement(<1..267)
                     /locus_tag="PLK08_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53668.1"
                     /translation="MLQRPAPNSILIARNFIMAIMRHIGEIGENVRKTARNFKWPPNV
                     KNWLRFVLRLVKIALSLYTSGVCFRQSRTYPKNMGVAEIEQFCWL"
     gene            367..840
                     /locus_tag="PLK08_08965"
     CDS             367..840
                     /locus_tag="PLK08_08965"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPS53669.1"
                     /translation="MIIRHARVPDAQDICRLVNYYAEQGLMLHRSLESVYECLREFFV
                     GEEAGKIIGCVAVDIYWANSAEIRSLAIAPDYRGKGAGKALVMQCLADAREIGLSQIF
                     AMTYNKKFFAAIGFEEVDIKTLPEKVWRECLEWYSKGHRHESAMLYNFTEADNAR"
     gene            830..1618
                     /locus_tag="PLK08_08970"
     CDS             830..1618
                     /locus_tag="PLK08_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPS53670.1"
                     /translation="MPDDRGRNVLISVRGVHKSFGPTPVLRGADIDIYEGQTTVVLGP
                     SGCGKSVLLKHMIGLLSCDEGCIKYDGNDVTHLRENHWAAIRREMGFLFQSAALFDSK
                     TVEQNIIFPMIYHSVGDDASRIQRCRDVLAMVGLVGMENRYPAELSGGQQKRVALARA
                     MILNPRIIFYDEPTTGLDPIRSDIINELILKLKHSMGNTAVVVTHDMTSARKVADRIV
                     MLHEGRFILDTTPQQIDHVQDENVRRFLEGRAGQKELCALGAVN"
     gene            1763..2755
                     /locus_tag="PLK08_08975"
     CDS             1763..2755
                     /locus_tag="PLK08_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPS53671.1"
                     /translation="MNEKNRNIAIGFTVIVALGILVVMILIFAGIPEALTGGTKIIIH
                     APHTGNLHKGDKIYLRGMRIGEITDVEFTQDNPTMGVTITGIIDNGICLPKNTLVSVE
                     TQGLTSMANLAVWPGNDDGRGFYKKGEVVVIDGIGQSGSSLIPKEVSDAMTDFAKLAR
                     SLNELVSGAATSQPAAISTTQNAATTTAPAIASTQPSADRPGLAGTVARLNRTLDSIY
                     SVSGNAQTQENIRQAIANLKVATDQMKTLLATADNLSRELITDAEQTGKLLKSFQKAV
                     DSLNEGNGTAGKLLNDPQVYNNLVEVTVELQGLIKDLRRLSDQWERNGVGVKLK"
BASE COUNT          814 a          781 c          730 g          596 t
ORIGIN      
        1 cagccagcaa aattgctcga tttcggcgac gcccatgttt ttgggatagg ttctactctg
       61 tcggaaacag acgccggaag tatacaaact taacgcaatc ttcacaagcc gcaaaacaaa
      121 tcttaaccaa ttcttaacat tcggtggcca tttgaagttt cgtgcagttt tacgcacgtt
      181 ttcaccgatt tccccgatat gccgcataat cgccattata aaatttcggg caattaagat
      241 gctgttcggg gctggccgct gcaacatttt acagaaatcc ggcaattccg ttcgggtaat
      301 cgattcattg atgcccgttc aagtttttgc cctgttattt aatgtcgata ttggtaaaat
      361 gcttccatga ttataagaca tgcaagagta cccgacgcac aagacatatg ccggctggta
      421 aattattacg ccgagcaggg ccttatgctt catcgttcgc ttgaaagtgt ttatgaatgt
      481 ctgagggaat ttttcgtcgg cgaagaagca ggcaaaatca tcggctgcgt agccgtcgac
      541 atttactggg ctaacagtgc cgaaatacgc tctttagcca tcgcgcccga ctatcgcggc
      601 aaaggcgcag gcaaggcatt agtcatgcaa tgccttgcag atgcccgcga aataggttta
      661 tcccagattt tcgccatgac ttacaacaag aaattctttg cagccatagg ttttgaagag
      721 gtggacatca aaacgctgcc ggaaaaagtc tggcgtgaat gtctcgaatg gtacagcaag
      781 ggtcatcgtc acgaatcagc catgctttat aatttcacgg aggctgacaa tgccagatga
      841 ccgcggcaga aacgtactca taagcgttcg cggcgtacac aaaagtttcg gcccgacgcc
      901 ggtgttgcgc ggagccgaca tcgacattta cgaaggccag acgaccgtcg tcctcggccc
      961 aagcggctgc ggcaagagcg tgctgctcaa acacatgata ggcctgctca gctgcgacga
     1021 aggctgcata aaatacgatg gaaacgacgt cacgcacctg cgggaaaatc actgggccgc
     1081 aatccgccgc gaaatgggat tcctcttcca gtcagccgcc ctgttcgact caaaaaccgt
     1141 cgagcagaat ataatatttc cgatgatata tcacagcgtc ggcgatgacg cctcccgcat
     1201 acagcggtgc agggatgtcc tggcgatggt gggattggtc ggtatggaaa accgttatcc
     1261 ggcggagctt tccggcggcc agcagaaacg ggtcgccctc gcgagggcaa tgatactcaa
     1321 ccccaggatt attttttacg acgaacctac gaccggcctg gacccgatac gctccgacat
     1381 aataaacgaa ctgatactga agctcaaaca ttccatgggc aacaccgccg tggtcgtgac
     1441 gcacgatatg acgtcggcac ggaaggtcgc agaccgtatc gtcatgcttc atgaagggcg
     1501 tttcatactc gacaccacgc cgcagcagat agaccacgta caggatgaaa acgtaaggcg
     1561 tttcctcgaa ggccgcgccg gccagaagga actctgcgca ctgggcgcag taaattgaca
     1621 aatggaaagt cggattttac gataccggaa gctgtttcaa agcatgcctt cgtcgcaagg
     1681 ccgagttttt tatgacagcc cggctacagc ggaaatgagt ttttaaaaaa agacacagct
     1741 tctacataca ggatagcctg acatgaacga aaaaaacagg aacatcgcaa taggttttac
     1801 ggtaatagtg gcgctgggca tattggtggt gatgatattg attttcgccg gcatacccga
     1861 agcgctgacc ggcggaacga aaatcatcat acacgcgccg cataccggca atcttcacaa
     1921 aggcgacaag atatacctgc gcggcatgcg aatcggcgag attaccgacg tggaattcac
     1981 gcaggataac ccgacgatgg gcgtgacgat aacgggcata atagacaacg gcatctgtct
     2041 gccgaaaaac accctcgttt ccgtcgaaac gcaaggcctt accagcatgg ccaacctcgc
     2101 ggtatggccc ggcaatgacg acggcagagg tttttataaa aaaggtgaag tcgtcgtaat
     2161 agacggcatc ggccagagcg gctcatcgct aatacccaag gaagtctccg atgccatgac
     2221 agacttcgca aaactcgcaa ggagtcttaa tgagctcgtg agcggcgctg cgacctcaca
     2281 gccggccgcc atctccacca cccaaaatgc cgccactacg acagcgccag ccatcgcatc
     2341 aacccagccg tcagccgaca gacccggcct cgccggaact gtggccagac tcaacagaac
     2401 cctcgactca atttattccg tcagcggcaa cgcacaaacg caggaaaaca tcaggcaggc
     2461 catcgccaat ctgaaagtcg caaccgacca gatgaaaacc ctgctcgcca cagccgacaa
     2521 tctttcgaga gaactcatca cggacgccga acagaccggc aagctgctca aaagtttcca
     2581 gaaagccgtc gactctctca acgaaggcaa cggcacagcc ggcaaactgc tcaacgaccc
     2641 gcaagtttac aacaacctgg tggaagtgac ggttgaactg cagggcctta taaaagattt
     2701 acgccgactc tcagaccaat gggaacgaaa cggcgtaggc gtcaagttga aatgaaaacg
     2761 gattttcgca cgctgcatta acacccagta ccagagttac cgccgtaaac ggaaatgcca
     2821 ataccaatgc gtaaaatcac cagccttaga gcattttaaa cttgctctag caacggcggc
     2881 tgatgcggat tgatgaatat atcaggctgc ggtggaaaag c
//
LOCUS       DAONFI010000304         2914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_43110_length_2914_cov_3.653026, whole genome shotgun sequence.
ACCESSION   DAONFI010000304 DAONFI010000000
VERSION     DAONFI010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43110_length_2914_cov_3.653026"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLK08_08980"
     CDS             complement(<1..606)
                     /locus_tag="PLK08_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53672.1"
                     /translation="MAENMQNTKPALGEETKAQTKGGVCGGMFEVSSFPTPPAGTFET
                     YRKMRGNPTVALARIAATAPIRAAEITVTAHADAPQAWKDFIDDQFKTLWPTLCKEML
                     RGLDYGFQAFEKVWDVADGMMVYRKLKPLLPEMTKIKTTDDHGIFAGLENNGTVLNVD
                     KSFIYTYDDEAGNPYGRSRHENIRETAWHPWNEISKKAEQYI"
     gene            complement(629..2080)
                     /locus_tag="PLK08_08985"
     CDS             complement(629..2080)
                     /locus_tag="PLK08_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HPS53673.1"
                     /translation="MIDPSAMLYDAWRWTPHTYARHASGGKWIPYPYLKLMGNMIAQT
                     VFDGGHLIVNLPPRHGKSELISFWTPVWFLDNFPDRNIILSSYGAELAEGFGRRVKNE
                     LQNNTCCRAKLSVDSKSSARLNTSEGGGMYSVGVGGPLSGRGGHLMIIDDPYKNWEDS
                     HSPAYRQTIRDWYRSTFRSRLEPGGAIIIAMTRWADDDLCGWLLEEFPEDWRVLSLPA
                     IAGENDVLDRKTGEALCRQRFDEEKLAQLRKESGSIVWDAMYQQKPKPMGIGVIYCNF
                     SAANIDTGCELIAGQPLHVSFDFNIDPGMHAEIGQYDNKADIFTCCHEIYDDRLSVRG
                     AMERFLKWFNTQNLKFPEIHVFGDASGDNEWAGTAESCYTIVRQYLDKLSIQYRMRVM
                     KKNPPVLERINTFNEALCDVDKKIHYRVHPRCKRLIADFKKLKSDAGGLIDKREHALS
                     HASDAEGYRVHYLRPLRSVSKMKDEARFIVSST"
     gene            complement(2077..2565)
                     /locus_tag="PLK08_08990"
     CDS             complement(2077..2565)
                     /locus_tag="PLK08_08990"
                     /inference="COORDINATES: protein motif:HMM:NF039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HPS53674.1"
                     /translation="MRTCLKKHGVIFSDKGKNPNSIKNLDLGKFKPGQSGNPAGRPKG
                     YASLSERLKLLLEKSVGDIITESEKTVILKNQGKGFLELSIADAIVKTAVRESLKGDF
                     KFIDMLWQRIDGKVAERIAGHDGGPLQTDSPAVNILAKAAGNPKLAKSIIELANACED
                     ES"
     gene            complement(2639..>2914)
                     /locus_tag="PLK08_08995"
     CDS             complement(2639..>2914)
                     /locus_tag="PLK08_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53675.1"
                     /translation="RLMSMADTQAEVKRSLADLSHHIAGNGKPGLTERVRKLEWWHGL
                     KVKAAVWLLGIAATIGTGYIVYKITRTEYHESETSKKEICTNQNASL"
BASE COUNT          639 a          761 c          676 g          838 t
ORIGIN      
        1 gatatactgc tccgcctttt ttgatatttc gttccacggg tgccatgcag tttcgcggat
       61 gttttcatgg cggcttcgcc cgtatggatt tccagcctca tcgtcgtacg tgtagataaa
      121 cgatttgtcg acgttcagga cagttccgtt attttcgaga cctgcgaaaa tgccgtggtc
      181 gtcggtagtt tttatcttgg tcatttccgg cagcagcggc tttaatttac ggtaaaccat
      241 cattccgtcc gcaacgtccc aaactttttc aaacgcctga aaaccgtaat cgagcccccg
      301 cagcatttct ttgcagagcg taggccagag tgttttgaac tggtcgtcaa taaaatcctt
      361 ccatgcctgc ggtgcgtcgg catgggccgt gacagttatt tccgccgctc gtatgggtgc
      421 agtggcggca attctggcaa gtgcgacagt gggattgcct cgcattttac ggtatgtctc
      481 aaacgtacct gcgggcggtg tcggaaaact cgacacctcg aacattccgc cgcaaactcc
      541 gcctttagtc tgggctttcg tctcttcgcc gagggccggc tttgtatttt gcatattttc
      601 agccattttt attaaatctc cagttcggtt atgtagagga tacgataaaa cgggcctcgt
      661 ccttcatctt ggacactgac cggagcggac gaagataatg cacgcggtat ccttcagcgt
      721 cgctggcgtg gcttaatgcg tgctcccgtt tgtcgataag accgcccgca tcgctcttta
      781 attttttaaa atccgctatc agacgtttgc atctagggtg tacgcggtaa tggatttttt
      841 tgtccacgtc gcatagcgct tcattgaacg tgtttatacg ttcaagtacc ggaggatttt
      901 tcttcataac ccgcatgcgg tactggattg acaacttgtc gaggtactgc ctcacgatgg
      961 tgtagcagct ctcagccgtg ccggcccatt cgttgtcccc tgatgcgtcg ccgaagacgt
     1021 gtatttcggg aaatttcaaa ttttgtgtat tgaaccattt taaaaaacgt tccatagctc
     1081 cacggacgct caatctatcg tcgtaaatct cgtggcaaca tgtgaaaata tcggccttgt
     1141 tgtcgtattg cccgatttcg gcgtgcatgc cagggtcaat gttgaaatcg aagctgacgt
     1201 gcagcggctg accggctatc aattcgcaac cggtatcaat attcgccgcc gaaaaattac
     1261 agtaaataac gccaataccc ataggtttcg gcttttgctg atacatagca tcccatacaa
     1321 ttgaaccaga ttcttttcgt agttgggcaa gtttttcctc gtcgaaccgc tgccggcaaa
     1381 gggcctcgcc tgtttttctg tcgagaacat cattttcgcc cgctattgcc ggcagactta
     1441 aaaccctcca atcctccggg aactcctcaa gtaaccagcc acaaaggtca tcgtctgccc
     1501 agcgtgtcat cgcaataata atcgccccgc ctggttcgag ccggctccga aatgtggagc
     1561 gataccaatc ccgtatcgtc tgtcgataag ccggactatg cgaatcctcc cagtttttat
     1621 acgggtcgtc gataatcatc aaatgcccgc cgcggccgct taacggcccg ccgacgccga
     1681 cgctgtacat cccgccgcct tcggatgtgt tcaggcgtgc ggacgatttt gagtccacgc
     1741 tcaatttcgc ccgacaacat gtattattct gcaactcgtt ttttactcgt ctgccgaatc
     1801 cctcggcaag ttccgccccg taactggaga gtatgatatt tctgtcagga aaattatcca
     1861 aaaaccatac cggcgtccag aatgaaatca gctccgattt gccgtgccgc ggcggaagat
     1921 tgacgattag atgtccccca tcaaaaacag tttgtgcaat catattaccc ataagtttaa
     1981 gatacgggta tggtatccat ttcccgccgc ttgcgtgtct ggcgtatgtg tgaggtgtcc
     2041 accgccacgc atcataaagc atggctgatg ggtcaatcat gactcatcct cgcatgcgtt
     2101 tgccagctca ataatgcttt tcgcaagttt tgggttgccg gcggcttttg ccaaaatatt
     2161 gacggctggc gaatcggtct gcaacgggcc gccgtcgtga ccggcaatgc gttctgcaac
     2221 tttcccgtca atccgctgcc agagcatgtc gataaactta aaatcaccct taagcgactc
     2281 gcggactgct gtcttaacaa tagcgtccgc gatggacagt tccagaaagc ctttaccttg
     2341 gtttttaagt atgaccgttt tttctgattc tgtaatgata tcgccgactg atttttccag
     2401 taacaatttc aatctttcgc tgaggctggc gtatcccttt ggccggccag ctgggttgcc
     2461 gctttgacca ggcttaaatt taccaaggtc gagatttttt atgctgtttg ggtttttacc
     2521 tttgtccgaa aaaattactc cgtgcttttt taggcatgtg cgcatagtag tagtgctgac
     2581 cttgtgtcta tccgccaatt caattaaagg cgtaccggag tcatacgatt cgatcagttc
     2641 agagacttgc gttttggttg gtgcaaattt cttttttact tgtttccgat tcatgatatt
     2701 ctgttcgagt aattttataa acgatgtaac cagtgccgat tgttgccgct ataccaagca
     2761 gccatacagc cgccttaact ttgagcccat gccaccactc caatttgcgg acacgctcag
     2821 tcaatccagg ctttccgttg ccagcaatat gatgtgacaa atcagccaac gagcgtttaa
     2881 cctcggcttg ggtatcagcc atcgacatta gtcg
//
LOCUS       DAONFI010000305         2901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_43392_length_2901_cov_4.668658, whole genome shotgun sequence.
ACCESSION   DAONFI010000305 DAONFI010000000
VERSION     DAONFI010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43392_length_2901_cov_4.668658"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="PLK08_09000"
     CDS             <1..1440
                     /locus_tag="PLK08_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPS53676.1"
                     /translation="ILEDIHWLGFDWGRHLYYASDYFDRMYEYAVELIKAGKAYVDDQ
                     TAEEISLTRGAPNVPGRESPYRNRSVEENLDLFTRMKNGEFPDGTKVLRAKIDMASPN
                     FNLRDPIMYRILHASHHRQGDKWCIYPMYDWAHGLEDSIEGITHSLCSLEFENHRPLY
                     DWFLNQLPHLHHPQQTEFARLNLTYTVMSKRKLLRLVREKFVSGWDDPRMPTLSGMRR
                     RGYSPQSIRNFCKLIGVNKFNSTVDMQLLEHCLRDDLNKTSARVMAVIDPLKVVITNY
                     PEGQVEELDAVNNPEDPTAGTRKVPFSRELYIERDDFLENPPKKFFRLSPGAEVRLRY
                     AYFVTCTDVVKDANGNITEIHCTYDPATRGGDSPDGRKVKGTIHWVSAEKAIDAEVRL
                     YEQLFTKENPEDVAEGEDFTVNLNPNSFKRITTCKVEPSLKKAEPLDRFQFERIGYFC
                     MDKDSAPDKPVFNRTVTLKDSWARIQQKL"
     gene            complement(1642..2022)
                     /locus_tag="PLK08_09005"
     CDS             complement(1642..2022)
                     /locus_tag="PLK08_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPS53677.1"
                     /translation="MEYSQKVLDLFENPRNVGTMDENDPDVGTGTQGSPACGDVMKVQ
                     IKVENGKITDAKFKTFGCCAAIASSSLATEWLKGKTLEEAATLKNSVLAEELQLPAVK
                     VHCSVLAEEAVKAAINDYNTKHQK"
     gene            complement(2050..>2901)
                     /locus_tag="PLK08_09010"
     CDS             complement(2050..>2901)
                     /locus_tag="PLK08_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPS53678.1"
                     /translation="ITLMLANNEIGTLHPVKEIGVVAHERGVIFHCDATQAVGKLPID
                     VQAMHMDLLSMSGHKLYAPKGIGALYVRRRNPVVQLAIQMHGGGHERNMRSGTLNVPG
                     IVALGKACEIAATEMTDEWTKMLKLRDRLEKGLMSRIKYVKINGHPTERLANTSNLSF
                     AYAEAESIFMRARDIAVSSGSACTSATLKHSYVLDAIGVSPAMANCSLRISMGRFNDE
                     ADIDYAVEKIAGSVQMSRDLNPDYETHLRLGILDENNPDTWMPSEEKCKSLCSDKLCC
                     RLKNLTD"
BASE COUNT          640 a          755 c          781 g          725 t
ORIGIN      
        1 attcttgagg acattcactg gctgggtttc gactggggca gacatcttta ctatgcgtcg
       61 gattatttcg acaggatgta cgaatacgcc gtcgaactca tcaaagccgg caaagcctac
      121 gtggacgacc agaccgctga agaaatcagc cttacccgcg gcgccccgaa cgtgcccggc
      181 cgtgaaagtc cgtatcgcaa ccgcagcgtc gaagagaatc tcgacctctt cactcgcatg
      241 aagaacggcg aatttcccga cgggactaag gtgttgcgcg ccaagataga catggccagc
      301 ccgaatttca atcttcgcga ccctattatg tatcgcatat tgcacgcctc tcatcatcgc
      361 cagggcgaca agtggtgcat ttatccgatg tacgactggg cgcacggcct tgaggacagc
      421 atcgagggca taacgcattc actctgttcg ctggagtttg aaaatcatcg gccgttgtat
      481 gactggtttt taaatcagct gccgcatctt catcatccgc agcagactga gttcgcgcgg
      541 ctgaatttga cgtacacggt gatgagcaaa cgtaagctgt tgcggctggt gcgggaaaaa
      601 ttcgtcagcg gctgggacga cccccgcatg ccgacgcttt ccggcatgcg ccgccgaggt
      661 tacagcccgc agtcgatacg caatttctgc aagttaatcg gcgtaaacaa gttcaattcc
      721 accgtcgaca tgcagctcct cgaacactgc ctgcgcgacg acctcaataa aacgtcggcg
      781 cgcgtaatgg ctgtaatcga cccgctgaag gtggtcatta cgaattatcc tgaagggcag
      841 gtcgaggaac ttgacgccgt gaataatccg gaagacccga cggctggcac gcgtaaagta
      901 ccgttcagcc gcgaactgta catcgaacgc gacgactttt tggaaaatcc gccaaagaaa
      961 tttttccggc tgtcgccagg cgcggaagtg cggctgagat atgcgtactt cgtgacctgt
     1021 acggatgtgg tcaaggatgc gaacggcaat attacggaaa ttcactgcac ctacgacccg
     1081 gctacgcgcg gcggagattc gccggacgga cgcaaagtca agggcacgat tcactgggtg
     1141 tcggcggaaa aggcgattga tgcagaggtg cgtctatacg agcagctttt tacaaaagaa
     1201 aatcccgaag atgtcgccga aggtgaagat tttaccgtaa atctgaatcc gaattcgttt
     1261 aaacgcataa cgacgtgcaa ggttgagccg tcgcttaaaa aggcagaacc gctcgacaga
     1321 ttccagtttg agcgaatcgg ttatttctgc atggataagg attccgcgcc ggataagccg
     1381 gtcttcaacc gcacggttac gctcaaagat tcctgggcca ggattcagca gaaactctga
     1441 tgataaaaaa ttcaggtgtt acccgtcaga catgccggta gagatttgct ttgattatat
     1501 taattgtagc taacggcgta ttatgcaggg cttttttgta cacatctcac tgccatgcct
     1561 gttgcggttg gctggcgtga tttggtgtaa aaaaatgtcg gcagggcgtg ttatatcatc
     1621 ggcttttgca gcggtaaaaa atcatttctg atgtttcgtg ttgtagtcgt tgatggcggc
     1681 ttttacggcc tcttccgcca gcacggaaca atgaactttt acagcaggca gctggagttc
     1741 ttccgccagc acggaatttt tgagcgttgc ggcttcttcg agcgttttgc ccttcagcca
     1801 ttccgtcgcc agcgagcttg atgcgatagc ggcgcagcag ccgaatgtct tgaacttcgc
     1861 gtccgtgatt ttcccgtttt ccacttttat ctggactttc atgacgtcgc cgcaggctgg
     1921 cgagccctgc gtgcctgtgc cgacgtccgg atcattctcg tccattgtgc cgacattccg
     1981 cggattttca aacagatcca agactttttg tgaatattcc ataaaacact tcctgtgatt
     2041 tgaataaatt taatcggtca gattttttag ccggcagcat agcttgtcgg aacagaggct
     2101 tttgcatttc tcttccgacg gcatccacgt gtccgggttg ttttcgtcca gtattcccag
     2161 ccgcagatga gtctcgtaat cgggattcaa atcccgcgac atctgtactg agccggcgat
     2221 tttttcgact gcgtaatcga tgtcagcctc gtcgttgaag cggcccatcg atattctcag
     2281 cgagcagttc gccatagccg gcgatacgcc gatggcgtcc agcacgtagc tgtgtttaag
     2341 cgtggcgctc gtgcaggctg agccgctgga aaccgcgatg tcgcgggcac gcatgaaaat
     2401 gctctccgcc tccgcatatg cgaaactgag attggacgtg tttgccagcc gctcggtagg
     2461 atggccgttg atttttacat attttattct gctcatgagg cctttctcaa ggcggtcgcg
     2521 cagcttgagc atttttgtcc attcgtcagt catttcggtg gctgctattt cgcaggcttt
     2581 gccgagtgcg actatgcccg gcacgttcag tgtgccgctt cgcatgttcc tctcatggcc
     2641 gccgccgtgc atctgtatcg caagctgtac caccggattg cgacggcgga cgtaaagtgc
     2701 gcctatgcct tttggggcgt agagcttgtg gccggacatg ctcagcaaat ccatgtgcat
     2761 agcctgaacg tcgatgggca gtttgccgac ggcttgagtg gcgtcgcagt ggaaaatcac
     2821 gccgcgctcg tgggctacta cgccgatttc cttcaccgga tgtaatgtgc cgatttcatt
     2881 attagccagc atcagcgtga t
//
LOCUS       DAONFI010000306         2869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_44144_length_2869_cov_3.524520, whole genome shotgun sequence.
ACCESSION   DAONFI010000306 DAONFI010000000
VERSION     DAONFI010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44144_length_2869_cov_3.524520"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLK08_09015"
     CDS             complement(<1..561)
                     /locus_tag="PLK08_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS53679.1"
                     /translation="MSKYLVTGGAGFIGSNLVRYVLNKGCEVVVLDNLATGKKENLTE
                     VMDSIEYVEGDVRDYDTCVKAMAGCTAVFHLAALGSVPRSVEEPRTSHDVNVNGTVNI
                     LEAARSLGIKRISFSASSSAYGDQKVSPKVETMAPAPISPYAANKLACEGYMQAYAAA
                     YGMETVSLRYFNVFGPRQDPGSPYSGV"
     gene            complement(586..1551)
                     /locus_tag="PLK08_09020"
     CDS             complement(586..1551)
                     /locus_tag="PLK08_09020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPS53680.1"
                     /translation="MKILITGGAGFIGSHLAGHFLSSGDEVTVLDNLSTGRLENVEIF
                     SQNPRYFFVKGDVCDAAVISPLIKNCDMIYHLAAAVGVQLIVEQPVHTITTNIFGTET
                     VLKAAAEFDKKLMLASTSEVYGKNSKIPFSEEDDTTLGCTRLNRWSYACSKMIDEFLA
                     LAYHEKYSLPVVICRFFNTIGPRQTGRYGMVVPRFVRSALAGRPIEIYGTGRQSRCFC
                     NVSDIIRAMTSLMACPEAYGRVINVGSRELITIIDLAKKVIELTGSASEIKYLTYEQA
                     YGRAFDDMQTRMPDLSRIGELIGYSPQKNLDETLQEIIAFEKEQC"
     gene            1645..2634
                     /locus_tag="PLK08_09025"
     CDS             1645..2634
                     /locus_tag="PLK08_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPS53681.1"
                     /translation="MNKRIAKILSMTEYRVNLEIYNGPLDLLLYLIRRDEVDIYDINI
                     THITEQYMHYLEILQQLDPNLAGDFLVMAATLMEIKSRMLLPGPEIAEGGEDELNIDP
                     RADLVRQLLQYKAFKDAAGELSHAAEMQSLRYPRKPALPDAENEKEIDIEDAQIWDLF
                     DAFRNVMSSIGATTRNHEVIYDDTPLELHQADLLDRLQRYGKMTFRQVFEGITRRTEV
                     VGLFLAMLELCRLRQVAVLQDSIFGEIYLEHVNPDELEINRPQNMVTTETGGIGFDDD
                     GEGVWHVADVDVQTELADNGDDDEKYDDNLDDIKVDFDIDEKLEHGAVNDDGK"
     gene            2621..>2869
                     /locus_tag="PLK08_09030"
     CDS             2621..>2869
                     /locus_tag="PLK08_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPS53682.1"
                     /translation="MTASRKLAGMGLALPQMAEPVGAYVPAVRAGSLVFTSGQLPLSH
                     GELIARGKVGRDVSITEAQAAARQCVLNALAAVAYICGS"
BASE COUNT          721 a          732 c          739 g          677 t
ORIGIN      
        1 gactcctgag tacggagacc cgggatcctg ccgcggcccg aaaacgttga agtaacgcag
       61 cgaaacggtc tccatgccgt aagccgcggc atatgcctgc atgtaaccct cgcaggcgag
      121 cttgttggcg gcatacggac ttataggcgc aggagccatc gtttcaacct tgggcgatac
      181 tttctggtcg ccgtaggcgc tgctcgacgc ggaaaaactg atacgtttga tgccgagaga
      241 gcgggcggct tccagaatgt tgacagtgcc gttaacgttc acgtcgtggc tggttcgcgg
      301 ttcttcgacg ctgcgaggca cagaacccaa tgccgccaga tgaaatacag cagtacagcc
      361 agccattgcc tttacgcaag tgtcataatc ccgcacatcg ccctcgacgt actctatcga
      421 gtccatcact tccgtgagat tttccttctt gccagtagca agattgtcca gcacgacaac
      481 ttcgcaacct ttgttcagta cgtatcttac cagattactg ccgataaaac cggctccgcc
      541 agttacaaga tattttgaca tttcttcgag tcctttatat agaaatcagc attgttcttt
      601 ttcaaaagct ataatttcct gcaacgtctc gtccaggttc ttctgtggcg aatagccgat
      661 aagctcgcct attctcgaaa ggtccggcat tcgcgtctgc atgtcgtcaa acgccctgcc
      721 gtaagcctgc tcgtaagtca gatattttat ttcgctggca ctgccggtca actcaataac
      781 tttttttgcc aggtcgataa tcgtaatcag ctcacgagaa ccgacgttta tcactctgcc
      841 gtatgcttcc ggacaggcca tcaatgaagt catggcgcgt atgatatcgg aaacgttgca
      901 gaagcagcgg ctttgtcggc ctgtgccgta aatctcgata ggccggcccg ccagggcgct
      961 tcgcacgaac cgcggcacga ccatgccgta gcggccggtc tgacgggggc ctattgtatt
     1021 gaaaaatcga cagataacaa ccggcaacga atatttttca tgatacgcca acgccagaaa
     1081 ttcgtctatc atcttgctgc atgcgtaact ccagcgattc agacgggtac agcccaatgt
     1141 ggtatcgtcc tcttcgctga atggaatttt ggaatttttt ccataaacct ccgacgtgga
     1201 tgccagcatg agttttttgt cgaattcagc ggcggctttc aggacggttt ccgtgccgaa
     1261 tatattggtg gttatggtat gcacgggctg ttcgacgata agctgtacgc ctacggcggc
     1321 ggcgagatga tatatcatat cgcaattttt tatgagaggc gagataacgg ctgcgtcgca
     1381 aacgtcgccc ttgacaaaaa aataacgtgg attctgactg aaaatctcca cgttttccag
     1441 cctgccggtg gaaagattgt ccagcaccgt cacttcatcg ccgctgctta aaaaatgacc
     1501 cgcaaggtgc gaacctatga aaccagctcc gcctgttata agtattttca aaactgtaac
     1561 ctcgtggcgg catttccgcc gtaaaacaaa atgaaaacat attttattgc ccacagccgc
     1621 aagtgcaaac aatttgtatg gtaaatgaac aaaaggattg ctaaaattct ttccatgact
     1681 gaatatcgtg taaatcttga aatctacaat ggcccgctgg acctgctgtt atatctcatc
     1741 cggcgcgacg aagtggacat atacgatatc aacataacgc atataaccga gcagtatatg
     1801 cattatctgg aaattcttca gcagctcgac ccgaatctgg caggcgattt cctggtaatg
     1861 gcggcgacgc tgatggaaat aaagtccaga atgctgcttc ccggccctga aatcgcggaa
     1921 ggcggcgaag atgaactcaa catcgacccg cgagccgacc ttgtgcgtca acttcttcag
     1981 tataaagcat ttaaagacgc tgccggcgaa ctttcccacg cagcagaaat gcaatcgttg
     2041 cgttatccgc gaaaaccggc tctgccggat gccgaaaacg aaaaggaaat cgatatcgaa
     2101 gacgcccaga tatgggatct tttcgatgcg ttccgcaacg tgatgagttc aatcggggcg
     2161 acaacccgca atcacgaggt gatttacgac gataccccgc tggaacttca tcaggctgac
     2221 ctgctcgacc ggttacagcg atatgggaaa atgacgtttc gacaggtttt cgagggcatt
     2281 acgcgacgaa ctgaagtcgt gggccttttc ctggctatgc tggaactctg tcgcctacgg
     2341 caggtagctg tcctgcagga ctccattttc ggcgaaatat atctggagca cgtcaatccg
     2401 gacgaacttg aaatcaatcg gccgcagaat atggtcacca cggaaacagg cggtatcgga
     2461 tttgatgacg atggcgaagg tgtatggcac gtcgccgatg tagacgtaca gacggaactt
     2521 gccgacaacg gcgatgacga tgaaaaatat gacgacaatc tcgacgatat aaaggtggat
     2581 tttgatatcg acgaaaaact tgaacatgga gcagtcaacg atgacggcaa gtagaaagct
     2641 ggctggcatg ggactggcgc tgccacagat ggctgaaccg gtcggggcgt atgttccggc
     2701 ggtgcgggct ggcagtctgg ttttcacgtc tggccaactg ccgctgagcc acggcgaatt
     2761 aatcgccagg ggcaaggtcg ggcgggatgt atcaataact gaagcccagg cggcggctcg
     2821 acagtgcgtg ttgaacgcgc tggctgccgt agcgtacata tgcggctcg
//
LOCUS       DAONFI010000307         2864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_44242_length_2864_cov_4.110360, whole genome shotgun sequence.
ACCESSION   DAONFI010000307 DAONFI010000000
VERSION     DAONFI010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44242_length_2864_cov_4.110360"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLK08_09035"
     CDS             <1..889
                     /locus_tag="PLK08_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPS53683.1"
                     /translation="TNQWDTELLEAVGIPSNILPPIADAPTILGKIKADNAPHPKLAG
                     VPVVISAGHDTAAASFAAGPLDEHSLFLSEGTYAMLGCISEKSAISAKTADSGCACSE
                     LARRQWGLFASITGLWLFQECCKQWEQNGWPVSYDTLAKDAMISRYRGRIPLSSAGVD
                     FSGDACGRLVDACANAGLKPPADKSEFARLIFESMAEEHAAAAQTLAKLTGHDFRNIR
                     MVSGGSRNTCLSQLLANALELPLAAGPAEASTIGNIMLQARAMGVVTSDNDAFDIIKN
                     SFPSIIYNVERPFNHELPR"
     gene            873..2357
                     /locus_tag="PLK08_09040"
     CDS             873..2357
                     /locus_tag="PLK08_09040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPS53684.1"
                     /translation="MNYLDELILLRNSYRNVKAALPWLDASVWQGEKTDFPNCAKSLQ
                     TPCVPAETISPSQAGQIASIKAGHGNCIFRYNPSISGSLETISPLMPELASARIPLVI
                     LHTDMPISEIRDFAKSYPAATFILESGPRKILYFISQVAELLTSCPNVFLSTYNFSNW
                     MGIEQFCQKGFASRLLYGSHWPKYDTGVAMGPIITANIPWEAKCDIAGNNMRRLLGQQ
                     AMTTKETPFIPASPFIIDSHCHNIDAGVQSPCNFPLPDEHMSPADWIDFMNRLSIDKI
                     FSTPTLPLYGLDSASHASQKMRKYAPSRFYYYEVFNPNIAAEAERLATDVKKSGCAGI
                     KIHPSQHNVAADSDAYSPVYELAARYNLPILTHSWEISEYNPCQYLSHPDRFRKHLER
                     HKNIHFILGHAGGRPSAIDATTKLCKDFPNVVADLAGDYFHNGVVEAMVERLGNSKVL
                     FASDMNWFDPSCMLGAVLGSSLPDKHVLNILRNNATAFFSVIHK"
     gene            2654..>2864
                     /locus_tag="PLK08_09045"
     CDS             2654..>2864
                     /locus_tag="PLK08_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53685.1"
                     /translation="MNQFEVDFKTPNRRYAIYPIIHDRIARQPDSTDSDEKISNVFNF
                     VWCRDLNAPELLAYYEKVGFGGVVGN"
BASE COUNT          767 a          721 c          677 g          699 t
ORIGIN      
        1 gactaaccaa tgggatacgg aacttcttga agcggtcgga attccatcaa acattcttcc
       61 gcctatcgcc gatgcaccga cgattttggg caagattaaa gcggataatg cgcctcatcc
      121 gaaacttgcg ggagttccgg tggtcatttc agccggccac gacaccgccg cggcaagttt
      181 cgcagccggc ccgctggatg aacattcgct gtttttgagc gaaggcacat atgcgatgct
      241 cggctgcatt tctgagaaat ctgccatatc cgccaaaaca gccgactcag gctgtgcatg
      301 ttcggagttg gcgaggcggc aatggggcct gtttgcgtcg attactggat tatggctgtt
      361 tcaggaatgc tgcaaacagt gggaacaaaa cggctggccg gtttcatatg acaccttagc
      421 caaagacgca atgatatccc gatacagggg cagaattccg ctgtcatcag ccggagtcga
      481 tttttccggc gacgcctgcg gcagactcgt cgatgcctgc gcgaatgcag gcctgaaacc
      541 gccggcggat aaaagtgaat tcgcccgcct gatttttgaa agtatggcag aagaacatgc
      601 cgcagccgcc caaacgcttg caaaactgac cggccatgat ttccggaata tccggatggt
      661 cagcggcggc agcagaaata catgcttgtc ccaactgctg gctaacgctc tggaattgcc
      721 tcttgccgcc ggcccggcgg aggcgtcgac aatcggcaat atcatgctac aggcacgtgc
      781 gatgggcgtg gtaacctcgg acaatgacgc atttgatatc ataaaaaatt ctttcccctc
      841 aatcatatat aatgttgaaa ggccttttaa tcatgaatta cctcgatgaa ctcatattac
      901 tacgcaactc atatcgtaac gtgaaggctg ccctgccgtg gctggatgca agcgtgtggc
      961 aaggagaaaa aacagatttc ccaaattgtg ccaagtcgtt gcagacgccg tgcgtccctg
     1021 ccgaaacaat cagtccctcg caagccggcc aaatagcttc cattaaagca ggtcacggta
     1081 attgcatatt ccgatacaac ccttcaatct caggttcact cgagacgata tctccgctca
     1141 tgccggaact ggcatctgcc cgaataccgc tggtaatatt gcacacggat atgccgatat
     1201 cggaaattcg cgattttgca aaatcatatc cggcggcaac cttcattctt gaatccggcc
     1261 ccagaaaaat actttatttc atctcgcagg tcgcggaatt gctgacgtca tgtccgaatg
     1321 tcttcctttc cacgtataat ttctctaact ggatgggaat cgaacaattc tgccaaaaag
     1381 gtttcgcgtc caggctgttg tacggcagtc attggccgaa atatgacaca ggcgtagcca
     1441 tggggccgat aataacggct aatatcccgt gggaagcgaa atgcgatata gccggcaata
     1501 acatgcgacg gctgctcggc caacaggcta tgacgacaaa agaaacgccg ttcatacccg
     1561 cttcgccgtt catcatagat tcgcattgcc ataatatcga cgccggcgtt caatctccct
     1621 gcaacttccc gctgccggat gagcatatgt cgcctgccga ctggattgac ttcatgaatc
     1681 ggctgtcgat tgataaaata ttctccacgc ctacacttcc gttatacggc ctggatagcg
     1741 ccagccatgc ttcgcagaaa atgcgtaaat acgcaccttc acgtttttat tactatgaag
     1801 tttttaaccc aaatatagct gccgaggcgg aacgacttgc gacggatgta aaaaagagcg
     1861 gctgcgcagg cataaagatt catccgtccc agcataacgt cgcagcagat tcggatgcgt
     1921 attcgccggt ttatgaactc gcggccagat ataatctgcc gattctcaca catagctggg
     1981 aaatatcgga atataatcca tgtcagtacc tttctcatcc cgaccgtttc cgcaagcatc
     2041 tcgaacgtca caaaaacatt cacttcattc tgggccatgc cggcggacgt ccttcagcca
     2101 tcgacgcgac aacgaaactc tgcaaagatt ttccaaacgt agtcgcagac ctggccggcg
     2161 actattttca taacggcgta gtggaagcca tggtcgaacg attgggcaat tcaaaggtgc
     2221 tcttcgccag tgatatgaac tggtttgacc ccagttgcat gctcggagcg gtattaggct
     2281 cgtcgcttcc ggataaacac gttttgaata ttttgagaaa caacgcaaca gcttttttct
     2341 ctgtaattca taagtaattt tctaccggtt agagtggttc tctaaagaat tttctttttg
     2401 agcgtcagcg aaagttttcc tgctaaggac gtcgaagcag gcaacctgca tggtttccga
     2461 tgcagacgga caatagcagg gaaacttgca gcaagcttca aaaggaaaat aattttgaga
     2521 accgctctaa accacgtaat aaaaaaatat tctttgggca gacacttttt aaaaccgtag
     2581 aatcccgaag gacatataaa aaaatgacgc attgtatgcg tcaataatcc attaacagta
     2641 aaaggaattt gttatgaatc agtttgaagt tgatttcaaa accccgaatc gtcgttatgc
     2701 gatatatcct atcatacacg acaggatagc tcgccagccg gattccaccg atagcgacga
     2761 aaaaatcagt aacgtgttta attttgtttg gtgccgtgat ttgaacgcgc ctgagttgct
     2821 tgcatattac gaaaaagttg gattcggcgg cgtagtcggc aacg
//
LOCUS       DAONFI010000308         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_44459_length_2855_cov_3.766786, whole genome shotgun sequence.
ACCESSION   DAONFI010000308 DAONFI010000000
VERSION     DAONFI010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44459_length_2855_cov_3.766786"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..516
                     /gene="tsaE"
                     /locus_tag="PLK08_09050"
     CDS             94..516
                     /gene="tsaE"
                     /locus_tag="PLK08_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPS53686.1"
                     /translation="MKFFISNSIDDTFQLGRRLGSRLAAGDCVALDGELGAGKTAFVR
                     GLAAGMGCDERLVSSPTYVLMQEYPGPIPLYHLDLYRMGDAAAEFTDMFPEELLENGV
                     MVIEWAGMAENVLPLPRTHITIKILSETSRKFTINEIA"
     gene            679..1467
                     /gene="truA"
                     /locus_tag="PLK08_09055"
     CDS             679..1467
                     /gene="truA"
                     /locus_tag="PLK08_09055"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPS53687.1"
                     /translation="MTDSVRNLMLVIAYDGRNYHGWQRQADGIETVQLRVEDALRHVL
                     RHPLTIQGCSRTDAGVHAQGQVVSVKTGNSAIPAEGLRRALNSRLPADIVVRSVRDVP
                     LSFNASGNACGKTYRYRIYTAPTRPVELAGQVYCYGRRLDAEKMRQAAARLTGRHDFA
                     AFASAGEERENTVRTIFSCTISDVGDEVEIWVRGDGFLYNMVRNIAGTLVEVGRGHWP
                     VSRIDDILAGCDRVLAGPTAPPDGLTLMCVHYPPDAFYEDNKNE"
     gene            1460..1759
                     /locus_tag="PLK08_09060"
     CDS             1460..1759
                     /locus_tag="PLK08_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53688.1"
                     /translation="MNNVNFSTTWKNILGLTAAGFLGMAVFVMIIIAISPILLIAGIW
                     LFIQKIKFNHMLRKAAGHGNVVEYAVDVNPSQNASDGFTAKHVDVTVIDESGNVL"
     gene            1812..2405
                     /locus_tag="PLK08_09065"
     CDS             1812..2405
                     /locus_tag="PLK08_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPS53689.1"
                     /translation="MTAYFISIFLKIFFLLTPFAAVSGFISMTSGCSSGEKRKIALQT
                     TVAMLAIVSLVFFFGQYIFDLFGITLNAFRVGGGALLFLAGVSLVRGGHAYEKASDED
                     DIAVVPMAIPLIVGPGTLAALFIMGAEVVNMREKMVALSAVLLAVLCVGGMLFLTVSV
                     ERHIKQKFIIMLTKITGLILASLAAQIIFDGIKGFLG"
     gene            2673..>2855
                     /locus_tag="PLK08_09070"
     CDS             2673..>2855
                     /locus_tag="PLK08_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53690.1"
                     /translation="MLCMSVRCPKCRTVLGVPDGYGGNRFLCGKCGTRFKVPALSDSE
                     IVDLIGIRNLDETSAGN"
BASE COUNT          743 a          689 c          744 g          679 t
ORIGIN      
        1 agcagccaag gttttgggga taggttctaa aaagactgaa atcctgtata tcatcgggcg
       61 catgtttttt gcgcattatt tcacatcaca gccatgaaat tttttatatc caacagcata
      121 gatgacactt ttcagctggg ccgccggcta ggcagccgcc tggccgcagg tgactgcgtc
      181 gcgctggacg gcgaactcgg cgcaggcaag acggcgttcg tacgcggact ggcagcgggc
      241 atgggttgcg acgaacggtt agtatccagc cctacatatg tgcttatgca ggaataccca
      301 gggccgatac cgctgtatca ccttgacctc taccgtatgg gcgacgcagc cgccgaattt
      361 acggacatgt tcccggaaga actgctcgaa aacggcgtca tggtcataga gtgggccggc
      421 atggcggaaa atgtcctccc cctgccgcga acccatataa ccataaaaat actctccgaa
      481 accagccgca aattcacaat caacgaaatc gcatgataaa aaaccccgca aaaaccggcc
      541 gacttcggga gcggttctcg aaatcatttt aaaacttcaa aaatatttta aaatttcaga
      601 cttattggtt atgtttacat ttcggatatt cgggattaat ttttttgtac tatcaggcta
      661 caggttaaaa ttatcaggat gaccgattca gttcgcaatc tgatgcttgt catagcgtat
      721 gacggcagga attatcacgg ctggcagcgg caggctgacg gcatagagac ggtacagctt
      781 cgcgtggaag acgcgctcag gcacgtgctg cgtcatccgc tgacgataca gggctgcagc
      841 cgcactgatg ccggcgttca cgcgcaaggc caggttgtca gcgtgaaaac cggcaatagc
      901 gcaattccgg cggaaggcct ccgtcgggcg ttaaattcac gactgccggc ggatatcgtc
      961 gtcagaagcg tgcgcgacgt gccgctgtca ttcaatgcgt cgggaaatgc gtgcggcaag
     1021 acgtatcgtt accgcatata taccgcaccg actaggccgg tggaactggc tggccaggtt
     1081 tactgttatg gccgacgtct cgatgcggaa aaaatgcgac aggctgcagc cagactcacc
     1141 ggcagacacg acttcgcagc cttcgcctcc gccggcgaag aacgcgaaaa taccgttcgt
     1201 acaatctttt catgcacaat cagcgacgtc ggcgacgaag tggaaatatg ggtacgcggc
     1261 gacggttttt tatataatat ggttcgcaat atcgccggca cgctggtgga ggtcggcaga
     1321 ggccactggc cggtcagccg gattgatgat atacttgccg gctgtgaccg tgtgttagcc
     1381 ggcccgacag ctccgccgga cggcctgacg ctcatgtgcg tacattatcc gccggacgct
     1441 ttttacgagg ataacaaaaa tgaataacgt aaatttttca accacatgga aaaacatact
     1501 gggattgacg gcggctggat ttctcggcat ggcggtcttc gtcatgataa ttatcgccat
     1561 atcgccgata ctgctgattg caggcatatg gctgttcatc cagaaaatca aattcaacca
     1621 tatgctgcgc aaggcagccg gacatggaaa cgtcgtggaa tacgccgtag acgtaaatcc
     1681 gtcacaaaac gcatcggacg gctttacggc caaacacgtc gatgttacag taatcgatga
     1741 gtcgggcaat gtactataaa aatagctgcc tgtttcattt tatcagacgc cattttaaaa
     1801 aagggtaaaa aatgaccgca tatttcataa gcatattttt aaaaatattt ttcctgttga
     1861 cgccttttgc cgctgtttca ggattcattt ccatgaccag cggctgcagc tccggcgaaa
     1921 agcgtaaaat agccctgcag acgaccgtcg ccatgcttgc gatagtatcg ctggtatttt
     1981 ttttcgggca atatattttc gacctctttg gcattacgct gaacgcgttc agggtcggcg
     2041 gcggcgcgct gctgttttta gccggcgtaa gccttgtacg aggcggccac gcatatgaaa
     2101 aggcgtccga cgaagacgat atcgccgtag tgcctatggc tataccgcta atcgtaggcc
     2161 ctggcacgct ggcagcgctg ttcatcatgg gtgccgaagt cgtgaacatg cgagaaaaaa
     2221 tggttgcgct gtcggcagtg ctgctggcgg tactatgcgt cggcggcatg cttttcctga
     2281 cagtgtcagt ggaacggcac atcaaacaga aattcataat catgcttaca aagataaccg
     2341 gccttattct tgcaagcctg gcggcacaga ttatctttga cggcataaaa ggctttttgg
     2401 ggtgacttcc ctacaaaaaa atatcctctg ctgccgataa aaaaaatatc aaaaaaattg
     2461 tcttcgagat gcgacaatgt tattatattc ataagaagaa gtttcgcaaa aaaacgcaag
     2521 agaggatggg cagcatcgtt attcgtatta aggcaggaat cgtaattcat ataactgaat
     2581 aactgtcgca taaaatgcaa aaagtggtgc tatgaagtct ccctcgttca ggcataatat
     2641 tttcatattt atcatagttc aggagacaga aaatgctttg catgagtgta cgttgtccca
     2701 agtgtagaac tgtgctggga gtgcccgacg ggtacggcgg caacaggttc ctgtgcggca
     2761 aatgcggcac aaggttcaaa gtgccggcat tgagcgactc cgagattgta gacctcatcg
     2821 gcattcgcaa tctggatgag acgtcggccg ggaat
//
LOCUS       DAONFI010000309         2852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_44517_length_2852_cov_3.914909, whole genome shotgun sequence.
ACCESSION   DAONFI010000309 DAONFI010000000
VERSION     DAONFI010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44517_length_2852_cov_3.914909"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1437)
                     /locus_tag="PLK08_09075"
     CDS             complement(151..1437)
                     /locus_tag="PLK08_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HPS53691.1"
                     /translation="MMITMHKKISLAMLVLFFMNLPLYAGVMKKYEAKYHIIYSDLPQ
                     EDVYEAISRVNAMAEQYHNRTKGFGGKITRKLPFFLYANYDDYQTAIGGQLAGSVGVY
                     TGKELRAAADSAKFSKEDVWHTVQHEGFHQFVDMVITNGRHDVMPIWLNEGMAEYFGE
                     AKWTGDKLIPGVVDTGKLFKKGKNLYKRSGRMQRVQESIKDGKFKPFPRMVTMSHDAW
                     NSQMDIANYDQAWSMVHFFVHAQDSAGNYKYRKKFQKYLESVVKGNDNAKSFCRYLGS
                     PTKLEREYNEWWLSWDKNPSQDICDRILIETLVNFMSRADAVCKMKFQKADDFFSAAK
                     KDKIKIDSSRFRNLWLPQSLLDEAVSQAELNNRDCTWDVKREKGKTVFIMIHKDGSEV
                     QCLYKTRGASLPLVKTTFISADDTGKKSKNADDDDD"
     gene            complement(1453..2181)
                     /locus_tag="PLK08_09080"
     CDS             complement(1453..2181)
                     /locus_tag="PLK08_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53692.1"
                     /translation="MNDNEKILKDGLRVSHYLWLGRVVMFLMLIIAGSMVFGGCAAVG
                     WFASLFPTADLPAQYEIPKDQSVLVLVEDPKNIVTYSPIKYELARSVNKLLEEHKACS
                     ELISPDSVLHLKSSCTEFSQLSTAEIGKKLDADLVLYIQITTFQLKDSPYDPVWNGKM
                     GATVRVISVNDGRLWPKDTLGGYPVDDVVRDRHMDTSDNYTEKLSADMAEELAQNIGE
                     LFYPHPAPEHTDLPKKQVNSLNQP"
     gene            complement(2222..>2852)
                     /locus_tag="PLK08_09085"
     CDS             complement(2222..>2852)
                     /locus_tag="PLK08_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53693.1"
                     /translation="AISAALSGCEGARWFTYLFQPREETLVKPEYPIDDKTVAVVVYT
                     DQATLYEYPNVRLTMGTKLCYELTHSLDDVKTISPFVVSRFQDDHVNWEAMDPRELCK
                     ELNADIILMVTLSEYRTREPGLMNTYQARILAEAKLYDKDSKDSEPVWELDKNIEVVY
                     PKEPHYSPQAEPLIAEQAEKMLAEDLVRKFYKHTYTVNDEGEKEYIKEN"
BASE COUNT          678 a          695 c          642 g          837 t
ORIGIN      
        1 cagtcgtttt tgttccttgc atctgcataa ttgccactca tttgcgtctc acagtctaat
       61 tgagaacgac ctctaggttc cggcgggaaa tatcgggcga tactgatacc tgtaataata
      121 tgcttttctt aactggaagc acttgccaaa tcaatcatca tcgtcatcgg cattttttga
      181 ttttttgccg gtatcgtcgg cggatatgaa tgtcgttttc acaagcggca gcgatgctcc
      241 ccttgttttg tacaaacact gcacttcaga accatccttg tgaatcatta tgaaaactgt
      301 tttgcctttt tcacgtttga catcccaggt acaatcacga ttattgagct cagcctgcga
      361 tactgcctcg tcaagcagcg actgcggcag ccagagattg cggaaacggc tggaatcgat
      421 ttttatcttg tcctttttcg ccgccgaaaa gaaatcgtca gccttttgaa atttcatctt
      481 gcaaactgcg tcggcacgcg acatgaaatt taccagcgtt tcaatgagta ttctgtcgca
      541 aatatcctgg cttggatttt tatcccatga cagccaccat tcgttatatt ccctttccaa
      601 cttggtcgga ctgcccagat acctgcaaaa acttttggcg ttgtcgttgc ccttcacaac
      661 tgactccaga tatttctgga attttttccg atatttgtaa ttgcctgccg aatcctgcgc
      721 atggacgaaa aaatgtacca tagaccacgc ctgatcgtaa ttggcgatat ccatctgcga
      781 attccatgcg tcgtgggaca tggtcaccat tctggggaaa ggcttgaatt tgccgtcttt
      841 tatggattcc tgcacgcgtt gcattctgcc gctgcgttta taaagatttt tgcctttttt
      901 aaacagtttg ccggtatcga ctacgccagg tatgagtttg tcgccggtcc atttggcttc
      961 gccgaaatat tccgccatgc cctcgttcag ccatatcggc ataacatcgt gtcggccatt
     1021 ggttataacc atatcgacga actggtgaaa accttcatgc tgcacggtat gccagacatc
     1081 ttcttttgaa aatttggctg aatcggcagc tgcccgcagt tccttgccgg tatagacgcc
     1141 cacgctgccg gcaagctggc cgccgatagc cgtctgataa tcgtcataat tcgcataaag
     1201 aaaaaagggc agtttgcgtg tgatttttcc gccaaaaccc ttggtacgat tgtgatattg
     1261 ctcagccatg gcgttgacgc gggagatggc ttcataaacg tcctcttggg gaaggtcgga
     1321 atatatgatg tgatatttgg cttcgtattt tttcattacg ccggcataca gcggcagatt
     1381 cataaaaaac aacaccagca tcgctaaaga aatttttttg tgcatcgtaa tcatcatcaa
     1441 aggcatccaa tattacggtt gattaagtga attgacctgc tttttgggta aatcggtatg
     1501 ttccggcgca gggtggggat agaacaactc cccgatattc tgggcaagtt cttccgccat
     1561 gtcagcagaa agtttttcag tatagttgtc ggaagtatcc atatgcctgt cccgaactac
     1621 gtcatccaca ggataaccgc caagcgtatc cttcggccat aatcggccgt cattgacgct
     1681 tatgactcga accgtcgcac ccatcttgcc gttccaaaca ggatcatacg gcgaatcctt
     1741 cagctggaac gtggtaatct gaatgtaaag aaccaggtcg gcatcgagtt ttttgcctat
     1801 ttcagccgtg gaaagctggc tgaattccgt acacgacgac ttcagatgca acaccgaatc
     1861 gggagatatg agttcggaac acgccttatg ttcttccagc agcttgttga cgctgcgggc
     1921 aagttcatat tttatcggtg aatacgtgac gatattttta gggtcttcca ccaaaacaag
     1981 gactgactga tctttgggaa tttcatattg cgcaggcagg tcggctgtag ggaacagcga
     2041 cgcaaaccat ccgacggctg cacagccgcc aaaaaccata ctgccggcta tgattaacat
     2101 caaaaacatg acaacacggc caagccacaa ataatgtgaa actctcaagc cgtcctttag
     2161 tattttttca ttgtcattca tcggtattgt acctgtaacg tcaaatccgc acataggtcg
     2221 gttaattttc ctttatatat tctttttcac cctcatcgtt aacggtgtat gtatgtttgt
     2281 aaaattttct caccagatct tcggcgagca ttttttccgc ctgttcggcg attaacggct
     2341 cagcctgcgg cgaataatgc ggctctttcg gatatacaac ctctatattt ttatccagct
     2401 cccacactgg ttcggaatcc ttggagtctt tgtcataaag tttcgcttca gccaatatcc
     2461 tggcctgata ggtattcatg aggcctggtt cgcgagtcct gtattccgaa agcgtaacca
     2521 ttagaatgat gtcggcgttg agttccttgc agagttccct ggggtccatg gcttcccagt
     2581 tgacgtggtc gtcctggaat ctggagacga cgaacgggct gatagttttt acgtcgtcaa
     2641 ggctatgggt gagttcgtag cagagttttg ttcccatcgt cagccgcacg ttgggatatt
     2701 catacaacgt agcctgatcc gtatatacga cgacggcgac ggttttatcg tctattggat
     2761 attcaggctt taccagcgtt tcttcgcgcg gctgaaaaag atatgtgaac caccgtgccc
     2821 cctcgcagcc gcttagggct gctgatatgg ca
//
LOCUS       DAONFI010000310         2824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_45126_length_2824_cov_3.992416, whole genome shotgun sequence.
ACCESSION   DAONFI010000310 DAONFI010000000
VERSION     DAONFI010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45126_length_2824_cov_3.992416"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..400)
                     /gene="acpP"
                     /locus_tag="PLK08_09090"
     CDS             complement(152..400)
                     /gene="acpP"
                     /locus_tag="PLK08_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPS53694.1"
                     /translation="MPTNAEIEAKVIEIVAEQMGVDKSEITRETSFVNDLNADSLDTV
                     ELVMEFEDAFDLSIPDEEAEKIQTVGQAIDHISEHCSK"
     gene            complement(555..1307)
                     /gene="fabG"
                     /locus_tag="PLK08_09095"
     CDS             complement(555..1307)
                     /gene="fabG"
                     /locus_tag="PLK08_09095"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPS53695.1"
                     /translation="MAKSKSVPDDQKIAIVTGGSRGIGFACCKELLSMGMTVIAAARS
                     AENLQKAAAELAGEKLETYVIDVADSAAVTKMIDYVVEKYGRLDVMVNNAGITRDQII
                     VMMEDADWNAVINVNLNSAFYGTREASKIMMRQRCGSVVNMSSFSGLEGNRGQANYAA
                     AKAGMIAMSKSAAKELAKRNVRVNCVCPGFIETDMTSVLPQEVKDIAQQIIPMQRMGT
                     ADEIAKAVAFLASDASSYVTGQTLSVDGGMHM"
     gene            complement(1387..2334)
                     /gene="fabD"
                     /locus_tag="PLK08_09100"
     CDS             complement(1387..2334)
                     /gene="fabD"
                     /locus_tag="PLK08_09100"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPS53696.1"
                     /translation="MAKTAFLFPGQGAQYVTMGKDICDAHECAREIFERAQAVTGLPI
                     MRLCFEGPEDELNATDIAQPAIFTVSAAMLAVMKKILGDDMPKPAVCAGLSLGEYTAL
                     YAAGAMDFETGVKLVARRGQLMQAAATARPSGMVAVMGLDEQQAVELCQKAAQGQILT
                     PANFNCPGQIVLSGENEPCQRAAELAPTMFPDACKGATVLKVAGAFHSEIMTPAAEEL
                     AKTLAEIKFNEPQIPVIANVDAKYYASAAEIPQKLLAQLTGAVRWQQSMEALLADGMD
                     APYEIGPGKVLFGLMRRISRDCKVTQVNELAKVENLKKC"
     gene            complement(2587..>2824)
                     /locus_tag="PLK08_09105"
     CDS             complement(2587..>2824)
                     /locus_tag="PLK08_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HPS53697.1"
                     /translation="MSAGPLAGVDAALSTMQMPPGIPVACMAIGSAGAQNAAIFAAEI
                     LGVTDTAIAEKLQAFKKEMAEKCAAKDAKLRQEL"
BASE COUNT          545 a          757 c          745 g          777 t
ORIGIN      
        1 aggcaggaac gtcgttggct acggggttaa ccgtacccag acccgttatt actacgcgtc
       61 ttttacccat gtaagggttc cttcctctgc gcaataccaa ccgtgcagcc gccggcacat
      121 ccaacggaag cacggttgat tgtcatactt cttacttgct gcaatgttct gaaatgtggt
      181 cgatagcctg gccgacggtt tgaatctttt cggcttcttc gtcgggtatc gacagatcga
      241 aagcgtcttc gaattccatg accagttcga ccgtgtccag cgaatcggcg ttgaggtcgt
      301 tgacgaatga ggtttcgcgg gtgatttcgc tcttgtcgac acccatctgc tcagcgacga
      361 tctcgattac ctttgcttcg atttctgcat tggtgggcac ttctattctc ctattaaagt
      421 tcgttgttaa gccggctaac atctgccata gccggcagcc cgttggcgtt acttcgggcg
      481 gttactatat acgctagcaa tgcccaaagc aaccattttt tatttttgcg cctaactccc
      541 tgtcaggcat gatattacat atgcattccg ccgtccacgc tcagtgtctg gccggttacg
      601 tagctgctgg cgtcgcttgc gaggaaggcg actgctttgg cgatttcgtc agctgtgccc
      661 atgcgctgca tggggattat ctgctgggcg atatccttga cttcctgcgg cagtacgctg
      721 gtcatgtcgg tttctatgaa gcccgggcat acgcagttga cgcgaacgtt acgtttggcc
      781 agttcttttg ctgcggactt gctcattgct atcatgccgg ctttggcggc ggcgtagttc
      841 gcctggccgc gattgccttc aagcccgctg aaactcgaca tgttgactac cgagccgcaa
      901 cgctgacgca tcatgatctt cgacgcttcg cgtgtgccgt aaaaggcgct attgaggtta
      961 acgtttatga cggcgttcca gtcggcatct tccatcatga cgattatctg gtcacgggtg
     1021 atgccggcgt tgttgaccat cacgtccagt cggccgtatt tttctacgac gtaatcaatc
     1081 attttggtaa ctgcggcgga atcggctacg tcgattacgt aggtttccag tttttcgccg
     1141 gcgagttcgg cggctgcctt ttgcaggttt tcagcgctgc gggcggcggc tatcactgtc
     1201 atgcccatcg agagcagttc cttgcagcac gcaaagccta tcccgcggct gccgccggtc
     1261 actatggcga ttttctggtc gtcaggtacc gattttgatt tagccatttt ttaatatctc
     1321 cgatttttcc tcagaaatcc gataaacttt tttcatcctg aatgatatcg tcgccgaagg
     1381 cggcatttaa cattttttca aattctcaac tttggcaagc tcatttacct gcgttacctt
     1441 gcaatcgcgg gaaattcttc gcataagtcc gaaaagcacc ttgcctggcc cgatttcata
     1501 tggagcgtcc atgccgtcag ccagcagcgc ttccatagat tgctgccatc gtaccgcgcc
     1561 ggttaattga gccagcagtt tttgcgggat ttcagctgcg gaagcgtaat attttgcgtc
     1621 aacattggcg atgacaggta tctgcggttc gttgaactta atttcggcga gagttttggc
     1681 gagttcttca gccgccggag tcattatctc gctgtgaaac gcgccagcca ctttcagaac
     1741 ggttgcgcct ttgcaggcgt ccggaaacat tgtcggggcg agttcggcgg cgcgctggca
     1801 gggctcattt tcgcccgaca gcacaatctg gccggggcag ttgaaattag ccggcgtaag
     1861 aatctggcct tgtgcagctt tctgacataa ctcgacagct tgttgttcgt caagtcccat
     1921 taccgcaacc atgccgctgg gccttgccgt agcagcagcc tgcattagct ggccgcggcg
     1981 ggcgacgagt tttacgccgg tttcaaagtc cattgcgccg gctgcgtaaa gagcggtgta
     2041 ttcgccgagg ctcaggccgg cgcagacagc aggtttcggc atatcgtcgc cgagtatttt
     2101 tttcattacc gcgagcatgg cggcggaaac tgtgaaaatc gccggctggg cgatgtcggt
     2161 cgcgttgagt tcgtcctcag ggccttcgaa gcataaccgc attatcggca gaccggtaac
     2221 cgcctgcgcc cgttcaaata tctcccgtgc acattcatgt gcgtcgcaga tgtccttacc
     2281 catcgtgaca tactgcgcac cctgccctgg aaacagaaat gccgtctttg ccataaaaaa
     2341 tcctttatat ttcgttatcc gccaatcagg gcggacaccg ttttgtcggc ccctgctgtg
     2401 ggggcctttt gctccgttta catccgtcgg agcagttgag ggacattatg cgaacaaaat
     2461 tacggttcgc aagcattttt tactgttttt aaaccaaaaa ttaacgacta gcccgaaagt
     2521 cgaacttcca tttgatgtgg gaaatccgaa taaattcccg atgattgttg taaaacgcgt
     2581 tggtatttac aattcctgcc gtagttttgc gtcttttgcg gcgcattttt cagccatttc
     2641 tttcttgaat gcctgcagtt tttcggcgat ggcggtgtcg gtgacgccga gtatttcggc
     2701 ggcgaagata gctgcgttct gagcgccggc tgagccgata gccatgcagg ctaccggaat
     2761 gccgggcggc atctgcatgg tgctcaatgc cgcgtccacg ccagccagcg ggccggctga
     2821 catc
//
LOCUS       DAONFI010000311         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_45352_length_2814_cov_3.488945, whole genome shotgun sequence.
ACCESSION   DAONFI010000311 DAONFI010000000
VERSION     DAONFI010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45352_length_2814_cov_3.488945"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLK08_09110"
     CDS             complement(<1..785)
                     /locus_tag="PLK08_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53698.1"
                     /translation="MRMPEKSKIQVSSICLIATLFCVFSPTSTAASKPWEAAAEVYNA
                     SLKLYKLNQWQDALAALQGFVEDFPTNENVPLAYLQIASCYNFMHKDDEELKILEKIT
                     KEFPDTMAAFYAWSTIYYDKFKKAQEVKSQAESGKFSKEFKDFVLFLENMEKQFGGLF
                     IAPINQIHWPVISDYYWHYSPGRITWWPYYEVLGYEIELYPLNGWNYWTTRFPDQRMT
                     QSILTLAKNPAMAEELLRVLQPCFRKLDDDLPACWKMMHVQLL"
     gene            complement(910..2103)
                     /locus_tag="PLK08_09115"
     CDS             complement(910..2103)
                     /locus_tag="PLK08_09115"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPS53699.1"
                     /translation="MKFFAIFLCVIASVELIEWLLRNILALKYWRAQQHLRPSSPCDL
                     DKTCPPTVSIVVAAKDEQDNIENCLRSLLSQDYPALEIICVNDRSTDATAAVVEKIMK
                     EDPRVRLINIEKLPDGWCGKNHAMQHGIRQSSGDWICMTDADCIMTAPNTISVAMRYA
                     LDEKADMISVVPTLKMHSFWETLLQPICGAVLMVWFPPMKVNDPAKSHAYANGMFMFI
                     SRGAYEKIGTHKAIAGSLIEDIDMARRVKNAGLCLRMIPSEDMISVRMYTSLSQIIRG
                     WVRIFIGSFPRLWPQTRALMVLIGKGLTLYIAAVLGWIMLAFGAEDVAWWKGCAIIGT
                     IGSFIETVMTCRFFIHVCGKWPLGMLYPLGCIMTASILGRAIFKHLTGGTIVWRNTSY
                     KAGPQ"
     gene            complement(2106..>2814)
                     /locus_tag="PLK08_09120"
     CDS             complement(2106..>2814)
                     /locus_tag="PLK08_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53700.1"
                     /translation="VGTVPPRNKMRQVIDAAGRLTDAYHGRTFMVQYLLDDNDLPSAI
                     ACGDPLPVHEMLTKQSAVSCGVVCGTQADILITNSHPRDHDLWQCFKAIPNTCWAARP
                     GGVVICLARCPEGLNEMKTLNWPLSPLWTRRIIRWIGAGNIAQMVDRMVTRIAGDSSW
                     FLRLATQILQRNPIYMVSPSLMVRSEGFPGIALFGTVEEALDSARRLLGGGPQRVAVY
                     PWGGASYPITADITVQQ"
BASE COUNT          706 a          742 c          643 g          723 t
ORIGIN      
        1 tcaagaagct gcacatgcat cattttccag catgccggca gatcgtcatc cagttttctg
       61 aagcacggct gaagtacgcg aagcaactct tcggccatcg ccggattttt cgccagcgtc
      121 agaatactct gagtcattct ctggtcagga aacctggtcg tccagtaatt ccatccattc
      181 aggggataaa gttctatctc atatcccaat acttcataat aaggccacca tgtaattctg
      241 cctggagaat aatgccagta ataatcacta atcaccggcc aatgaatctg attgattggt
      301 gcgatgaaca acccgccgaa ctgtttttcc atgttttcca gaaacagtac gaaatccttg
      361 aattcctttg aaaatttacc cgattcagcc tgcgatttca cctcttgggc ctttttaaat
      421 ttatcgtagt atatggtgct ccatgcataa aaagcagcca tcgtatcggg aaattccttg
      481 gttatttttt caagaatttt gagctcttca tcatccttat gcatgaagtt atagcaagag
      541 gctatctgga gatatgccag cggcacattt tcatttgtag ggaaatcttc gacgaaaccc
      601 tgaagagccg ccagagcatc ctgccactga ttcaatttat atagtttcaa ggatgcatta
      661 tagacttcgg cggcggcttc ccacggcttg gaggctgctg ttgaagtagg ggaaaaaaca
      721 caaaacaacg ttgcaatcag acaaatggaa ctgacttgaa tttttgattt ttccggcata
      781 cgcataaaaa ctctcctgcc atgaaagaca actttttaac cgtttccgcg ccctgcatac
      841 ataaagacgt tattctaagg tcattagaat caaaattcaa ataaaacttg gttttcggga
      901 tttgtggaac tattgagggc cggctttata ggaagtattt cgccagacta tcgttccgcc
      961 ggtaagatgt ttgaaaattg ccctgccgag tatgcttgcc gtcatgatac agccaagcgg
     1021 ataaagcatg cccagtggcc atttgccgca gacatgtatg aaaaacctgc aagtcatgac
     1081 ggtttctatg aagctgccaa tcgtaccaat gattgcacag cctttccacc acgcaacgtc
     1141 ttcagccccg aaggctaaca ttatccaccc cagtaccgca gcaatatata gcgttaaccc
     1201 tttgccgata agtaccataa gagcgcgcgt ctgcggccat aatcgcggaa acgagcctat
     1261 gaatatccgc acccagccgc ggattatctg ggaaagcgat gtatacatcc gtaccgatat
     1321 catatcttct gaaggtatca tccgcagaca gaggccggcg ttcttcaccc gccgcgccat
     1381 gtcgatatcc tctatcaaac tgcctgcgat agccttatga gtgccgattt tctcatatgc
     1441 cccgcgactt atgaacatga acatgccgtt tgcgtatgca tgcgatttgg cagggtcgtt
     1501 cactttcatc ggcggaaacc ataccatcag cacagcgccg catatcggct gaagaagcgt
     1561 ctcccagaat gagtgcatct taagcgtggg gactacgctt atcatgtcgg ctttttcgtc
     1621 cagcgcgtag cgcatcgcca cggaaatcgt attcggtgcg gtcattatgc agtcggcgtc
     1681 ggtcatgcaa atccagtcgc ccgatgattg acgtatgccg tgttgcatgg catgattttt
     1741 cccgcaccag ccgtccggca atttttctat atttatcagc cgtactcgcg ggtcttcctt
     1801 cattattttt tcgacgacag ccgccgtagc atccgtactg cggtcattca cacagataat
     1861 ctccaatgcg ggataatcct ggctaagcag cgaacgcagg caattttcga tattatcctg
     1921 ttcgtccttg gcggctacca caatgctgac tgtcggcggg caggttttat ctaggtcaca
     1981 tggcgaagaa ggccgcaaat gctgctgcgc ccgccagtat ttcaacgcca gaatgttccg
     2041 taacaaccat tcaatcagtt ctaccgacgc gataacgcac aaaaatattg caaaaaattt
     2101 cattcttact gctgcaccgt gatgtccgcc gtgattggat aactggcacc gccccacgga
     2161 tataccgcga cgcgttgagg gccgccgcca agaagccttc gggcggaatc gagagcttcc
     2221 tcaacagtgc cgaacaatgc tatgcctgga aaaccctctg accttaccat caacgagggc
     2281 gaaaccatat atatgggatt gcgctggagt atctgcgtgg caagccgcaa aaaccacgac
     2341 gaatctccgg caattctggt aaccattcta tcaaccatct gagcgatatt tcccgcgcca
     2401 atccatcgta ttattcttcg ggtccatagc gggctcaacg gccaattcag ggttttcatt
     2461 tcattcaaac cttccgggca gcgtgccaga catatcacta cgccgcctgg cctggcggcc
     2521 cagcaggtat tcgggatggc tttgaagcac tgccataaat cgtggtcgcg aggatgcgaa
     2581 ttggttatga gaatgtcagc ctgagttccg cagactacgc cgcatgatac tgcgctctgc
     2641 ttggtgagca tttcgtgtac cggcaacggg tcgccgcagg cgatggctga cggaaggtca
     2701 ttatcgtcaa gaagatactg taccatgaac gttctgccgt gatatgcgtc cgtcagtcgg
     2761 ccggctgcgt ctatcacctg acgcatttta ttccgcggcg gcacagtgcc cacc
//
LOCUS       DAONFI010000312         2809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_45477_length_2809_cov_3.360930, whole genome shotgun sequence.
ACCESSION   DAONFI010000312 DAONFI010000000
VERSION     DAONFI010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45477_length_2809_cov_3.360930"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1787)
                     /locus_tag="PLK08_09125"
     CDS             complement(60..1787)
                     /locus_tag="PLK08_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53701.1"
                     /translation="MKYLPLLAILISCCVIQADIVYLSDGSEVEGITSQNDGQVSVEL
                     RDGQKKTFSESEILYVAKGPLRDNPTPTQTAGTPQTQPSQQTTSDPTSQPATTKPVDE
                     KPNDDLGPVKIDSDSDESQAKLQKCARDPADMLYEYLRRKISSPTPGIEDLINTARIR
                     IHDRMYRIGSDWVKPEDFERHRQAFEKCLSEAQAIYKDAAKLSDKDEKESKQKQAMLE
                     NAQAKLLQAAKSWLDPLTRWFLLGIAAYNSQDYEKAIGNFSRCASQAPWVAAFFQGLG
                     DAMMAVNRHEDAMSPYIRMSELTDDRSVAANILRDALQKTPGTKIMSEKVVNAKKFLE
                     QLQQSMKSSRGGNSQRQAWLLPGKAVSCKPDSLPQLPFDMLTFKQGVAVPVTQQALLV
                     GTDIVKDADTVIIVLRSWLYVTAKVEIPRNEPIAILWIPSNIQLTPVKISPKNSLKRG
                     QLITAVTADISPAMSTQAREASANVKDIDTLGNVSLYGGLLPGEGTSPALDSSGELAG
                     FIAGKLDTDKDSFPDRLITLSSQSMVGRRLMRYSKYSKSRPNQPAGMNINGQSFMVFA
                     VGTLKLEKK"
     gene            1880..>2809
                     /locus_tag="PLK08_09130"
     CDS             1880..>2809
                     /locus_tag="PLK08_09130"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HPS53702.1"
                     /translation="MKKYFFLFNFAAICWISTAAAQFGQSLSSADSAVEPSVKVKVVA
                     DRQTVRAGQAVTIAVEVTIPDGWVYYGPVQKPDTVVPCSLNVTAGGMELEELRYPKPH
                     LKPTEWGQGVVEIPVYSGKVIFFAVLRAPADAEQGGDYKIVLEPQGQICRDVCVYIPV
                     LPAAVIVKVGAENIANPAWPADMDRLFADSFVTTQLHQATPAAGEEFRISGRSDYTLW
                     GAVWLAFLAGLLLNVMPCVLPVLPLRILSVVQMAGGSRRRYVTLGVAFAGGVLAFFAC
                     IAALNIVLRLAVGRAVDLNEGYQSPAVIGGLCAI"
BASE COUNT          555 a          698 c          807 g          749 t
ORIGIN      
        1 ccatacacag tttattacat ataaagtcaa aagtcaaaca tcgcgcatgc cgcgatatat
       61 catttctttt ccagtttcag cgttcctacg gcaaagacca tgaatgattg gccgttgata
      121 ttcattccgg caggctggtt gggacggctt tttgaatatt ttgaatatcg catcagccgc
      181 cggccgacca tgctttgcga cgagagcgtg ataagacggt ctggaaatga gtccttgtcg
      241 gtgtcgagct tgccggcgat gaagcctgcg agttcgcccg atgaatccag cgccggcgat
      301 gtgccctcgc caggcagaag gccgccgtac agggatacat ttcccaaagt gtctatatct
      361 ttcacgttgg cggatgcttc acgtgcctgc gtactcatcg cgggggatat atcagccgtc
      421 accgctgtga taagctggcc gcgcttcaga gaatttttgg gcgatatctt caccggggtc
      481 agttgaatgt tcgacggaat ccagaggatg gctatgggtt cgttacgcgg aatctccact
      541 tttgccgtga cgtacagcca gcttcgcagg acgattatga ctgtgtcggc gtccttgacg
      601 atgtctgtac cgacaagcag tgcctgctgg gtcaccggaa cagctacgcc ctgtttgaac
      661 gtgagcatgt cgaaaggcag ttgcggcagg gagtcgggct tgcagctgac ggctttgccc
      721 ggcagcagcc atgcctgccg ctgcgagttg ccgccgcggg atgatttcat gctttgctgg
      781 agctgctcga gaaatttctt ggcatttacg accttttcgg acattatttt tgtgccgggc
      841 gttttttgaa gggcgtcgcg cagtatattg gcggcgacag acctgtcgtc cgtaagttcc
      901 gacattctta tgtaaggaga catggcgtct tcatggcgat tgacagccat catggcatcg
      961 cccagtccct gaaagaatgc cgccacccat ggcgcctgcg aggcgcacct ggaaaaattc
     1021 ccgattgctt tttcgtagtc ctgtgaatta tacgcggcta tgcccagcag aaaccatcgc
     1081 gttagcgggt cgagccatga tttggcggcc tgcagcagct tggcctgcgc attttcaagc
     1141 atagcctgct tctgcttgga ctctttttcg tccttgtcgc tgagcttcgc agcatccttg
     1201 tatatggcct gagcttctga caggcatttt tcaaaagcct gacgatgccg ctcgaaatct
     1261 tcaggcttga cccaatccga gcctatcctg tacatgcggt cgtgtatacg gattctggcg
     1321 gtgtttataa ggtcttctat tccgggcgtc ggcgaagaaa tttttcgtcg aagatactcg
     1381 taaagcatgt cggcagggtc gcgtgcgcat ttttgaagtt ttgcctgcga ttcatcggaa
     1441 tcggagtcta tttttaccgg gccgagatcg tcgttgggtt tttcatcgac gggttttgtc
     1501 gtagctggtt ggctggtcgg gtcggatgtg gtttgctggg acggctgtgt ttgcggcgtg
     1561 cctgcggttt gtgtgggggt ggggttatca cgtagagggc ctttggcgac gtacagaatt
     1621 tccgattcgg aaaaagtctt tttctggccg tcccgcaact cgacgcttac ctggccgtca
     1681 ttctggctgg ttatgccctc tacctcactg ccgtctgaca ggtaaacaat gtcagcctgt
     1741 attacgcagc acgaaatcaa tattgccaaa agcggtagat atttcatttc aaaacctttc
     1801 ctgcagctgc acacatgaca ttttagtcat ctccgtctat aatgtgaaga atgaaaacat
     1861 tttaaatatc ggagagagca tgaaaaaata cttttttttg ttcaattttg cggcaatatg
     1921 ctggatttcg acggctgcag cccagtttgg ccagagtttg tcttccgctg attctgccgt
     1981 tgagccgtcg gtaaaagtga aagtcgtcgc tgaccgccag actgtccgtg ccggccaggc
     2041 tgttacgatt gcggtggaag tgaccattcc ggatggctgg gtatattacg gtcctgttca
     2101 gaagcccgac accgtagtgc cgtgttcact gaacgtcaca gccggcggca tggagctgga
     2161 agaacttcgc taccccaaac cgcatctcaa gcctaccgaa tggggccagg gtgttgtgga
     2221 aattcccgtg tattcgggga aagtcatatt ttttgccgtt ctgcgggctc cggctgacgc
     2281 tgaacagggc ggcgattaca aaatagtgct agaaccgcaa ggtcagatat gcagggacgt
     2341 ttgcgtgtac ataccggttt tgccggcagc cgttattgtg aaagtcggag cggaaaatat
     2401 cgccaatccg gcgtggccgg cagacatgga cagacttttc gccgattcat tcgtaaccac
     2461 gcagcttcac caggcgactc ccgcagccgg cgaagagttc cgcataagcg gccggtcaga
     2521 ttatacgttg tggggtgctg tttggctggc atttctggct ggcctgctgc tgaacgtaat
     2581 gccgtgcgtt ctgccggtac tgccgctgcg gatactttcc gtggtacaga tggccggcgg
     2641 ttctcgcagg cgttatgtga cgctgggcgt tgcctttgca ggcggcgtgt tggcgttttt
     2701 cgcatgcata gccgcattga atattgtgct gcggctggct gtcggcaggg cggttgacct
     2761 caatgaaggt taccagtcgc cggcggtgat tggcggactg tgcgcgata
//
LOCUS       DAONFI010000313         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_45530_length_2807_cov_4.605015, whole genome shotgun sequence.
ACCESSION   DAONFI010000313 DAONFI010000000
VERSION     DAONFI010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45530_length_2807_cov_4.605015"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLK08_09135"
     CDS             complement(<1..240)
                     /locus_tag="PLK08_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPS53703.1"
                     /translation="MSVKRKQFPVELKDVEITGGLIGQRAEKNAKISIADQYRKCLKT
                     GRIDAIRLKWKAGQPDKPHQFWDSDIAKWIEAASYS"
     gene            577..1692
                     /locus_tag="PLK08_09140"
     CDS             577..1692
                     /locus_tag="PLK08_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPS53704.1"
                     /translation="MDFVNQEFLSNKCFEWILLFLTIFIFMLAGKILSVFFEKQARHF
                     RCKKHFTTLAILFESMSKPIMLFSFGFGLKIGDAFIWMAQDVDLIYKRSVLFLLAISV
                     IWFFFRLVDVLEHMMLKVSHKAGSLLDVQLIPIIRKSMRIFVVIVGALYVTSKVFGGD
                     IGTILAGMGIGGLAFALAAIDMLANFFGSITILFDKPFRPGDHIRIGNYEGTVDEIGF
                     RSTRVRMLDGHYVSIPNQNMTNDAVENISIRPYIRRDFTVGIEYSSSPAQLSRAIGII
                     EEMLKSRQKDFPENKPGKVYFMSLASASLEIGVTYWYTPAQWFDYLSFSSDFNMELFE
                     RFCKEGISFAFPTQTLYLRKDSANDVVAGKSEGCKDA"
     gene            1689..2777
                     /gene="nadA"
                     /locus_tag="PLK08_09145"
     CDS             1689..2777
                     /gene="nadA"
                     /locus_tag="PLK08_09145"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPS53705.1"
                     /translation="MNKAVHDKPSAGELSARISDLRRELGQRVCILAHHYQKDEIVAQ
                     ADFVGDSLKLSQIAAGQNSQYIVFCGVHFMAESAEILAGENQTVVMPKTSAGCDMADM
                     ATADDMAAAIEILQKLDNGKHRVIPVTYVNSTAAVKAVTGQYGGSCCTSSNAGQVVRW
                     AMEQAGESGKIIALPDQHLIRNTAFQLGFTEDDICMFDHEMPEGGIDASTFRRSKFIL
                     WPGYCYVHQKFTPKHISAVRKEHPDVKVVVHPECPHEVVELADMAGSTEMMMSAINGS
                     PAGSCWAVGTESTFVGRLAKNNPDKTVLNLNPQKSFCEQMGKTDLVSLKNCLEAILGQ
                     KKSAGIIEVPYEEAVTARKALEKMISIV"
BASE COUNT          719 a          622 c          722 g          744 t
ORIGIN      
        1 cgagtagctt gccgcctcta tccacttggc gatgtcggag tcccagaatt ggtgcggctt
       61 gtcgggctgg ccagccttcc acttgagccg gatggcatcg attctgccag tcttaagaca
      121 tttgcgatat tggtcggcaa tgctgatttt ggcatttttt tcagcccgct ggcctatgag
      181 gccgccggtt atttcaacgt ctttcaactc gacaggaaat tgttttcgct ttactgacat
      241 ttttaaaact cccgtacgca taaaatgggt attatatttg aatttatcgg cttggcggcg
      301 gggtattccg cagaaaaatg gcaacaactt ccattatttg ctgatttcgt aagatgccgg
      361 ctgtcggaag atatgtcgta actcttttta tatcatatat aaaagaaacg gaccgggcgg
      421 ctagtttaat gggttatggc gttcaaatag aatggaaatt gacaggaaaa aggaataaaa
      481 tataaaaccg tttaacaatt atgccgggca atctgtaagc ggttgaaaat tcagactgga
      541 gatgcaatgg actggacggc gatttggaaa aatactatgg atttcgtaaa ccaggaattt
      601 ttgagcaata aatgctttga atggatattg ctgttcctta ccatattcat ttttatgctg
      661 gccgggaaaa ttctgtccgt atttttcgaa aagcaggctc gtcatttcag gtgcaaaaag
      721 catttcacga ccttagccat acttttcgaa tccatgagca agccgataat gctgttttca
      781 ttcggtttcg gcctcaaaat aggcgacgca ttcatatgga tggcacagga tgtggatctg
      841 atatacaagc gttcggttct gtttttgctg gcgatatccg ttatatggtt ttttttccgg
      901 ctggtggatg tgctcgagca catgatgctg aaggtttcgc acaaggccgg ttcacttctg
      961 gatgtccagc tcattccgat tatccgcaag tccatgcgga tttttgtggt tatcgtcggc
     1021 gctttgtatg ttacgtcaaa agttttcggc ggcgacatag ggacgattct ggctggcatg
     1081 ggaataggcg gcttggcctt cgctcttgcg gcaatagaca tgctggctaa ttttttcggg
     1141 tcgataacca tactttttga caaaccattc aggcccggcg accatattag aataggcaac
     1201 tacgagggaa cagtcgatga gataggattc cgttcgacgc gggtacgcat gctcgacggg
     1261 cattacgtgt cgattccaaa tcagaacatg accaatgatg ccgtggaaaa catttccata
     1321 aggccttata tccgccgcga ttttaccgtc ggcatcgaat attcatcctc gccggcgcag
     1381 ctcagccgcg ccatagggat tattgaagaa atgctcaaat cgcgacaaaa ggattttccg
     1441 gaaaacaaac ctggaaaggt atattttatg tcgctggcct cggcgagcct ggaaatcggc
     1501 gtgacgtact ggtacactcc ggcccaatgg tttgattatc tgagtttcag cagcgacttc
     1561 aatatggaat tgttcgaacg gttctgcaaa gaaggaattt catttgcatt cccgacccag
     1621 actttgtatc tccgcaagga ttctgcgaat gatgtcgttg caggcaaatc agaaggatgt
     1681 aaagacgcat gaataaagca gtgcatgaca agccgtctgc cggcgaactt tccgcccgca
     1741 tcagcgacct gcggcgtgag cttgggcagc gggtgtgcat attggctcat cactatcaga
     1801 aagatgaaat cgtggctcag gctgattttg tcggcgacag ccttaaactt tcgcagatag
     1861 ccgccggaca gaactcgcaa tacatagtat tttgcggcgt gcatttcatg gctgagtcgg
     1921 cggagattct cgccggtgaa aatcagaccg tcgtcatgcc caaaacttca gccggctgcg
     1981 atatggctga catggccact gcggacgaca tggctgccgc aatcgaaatt ctgcaaaaat
     2041 tggataacgg caaacatcgc gtgataccgg taacatacgt caattcgacg gctgccgtaa
     2101 aagctgttac cggacaatac ggcggttcat gctgcacgag ttcaaatgcc ggacaagtcg
     2161 tcagatgggc tatggaacag gctggcgaat caggcaaaat cattgccctg cctgaccagc
     2221 accttattcg caacacggca ttccagctgg gttttacgga agacgacatc tgcatgtttg
     2281 accacgaaat gccggaaggc ggtattgatg ccagtacttt cagacgttca aaattcatac
     2341 tttggccagg ctattgttac gttcaccaga agtttacgcc caaacatatt tcagccgttc
     2401 gcaaggagca tccggacgtt aaggtggtgg tgcatcctga atgtccgcat gaagtggtgg
     2461 aactggcgga tatggctggc agtactgaaa tgatgatgtc ggctataaac ggttcgccgg
     2521 caggttcatg ttgggccgtg ggtacggagt cgacgtttgt gggccggctg gcaaaaaata
     2581 atcctgacaa gacggtgttg aacctcaatc cgcaaaaatc tttctgcgag cagatgggca
     2641 agacggacct tgtttcgctg aaaaactgcc tggaagccat acttggccaa aaaaaatctg
     2701 ccggcattat tgaagtgccg tatgaggaag cagtcaccgc ccgaaaggcg cttgaaaaga
     2761 tgatatcgat tgtctgataa caaaatttta cggatttgta ttttcgg
//
LOCUS       DAONFI010000314         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_45574_length_2805_cov_3.336000, whole genome shotgun sequence.
ACCESSION   DAONFI010000314 DAONFI010000000
VERSION     DAONFI010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45574_length_2805_cov_3.336000"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..976)
                     /locus_tag="PLK08_09150"
     CDS             complement(176..976)
                     /locus_tag="PLK08_09150"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HPS53706.1"
                     /translation="MKPFLKWAGGKYKIIDRIIRVLPQGTRLIEPFAGSSAVFLNTKY
                     EKYLIADSNIDLINVYKQLKKDGTDFIAYCNELFIKANNKEKVFYRLREEFNTISDST
                     RKAALFIYLNRHCFNGLCRYNSRGLFNVPFGSYKNPPFPEKEMLDFVKKSKKAQFMAA
                     DFVETMGKAVVGDVIYCDPPYVPLTETSNFTNYTGSGFGQQEQDKLVALAKKLANKGI
                     PVIISNHDTDFTRNSYSSAQIDFFDVRRFISSKVTQRNKVSELLAVFA"
     gene            complement(1119..>2805)
                     /locus_tag="PLK08_09155"
     CDS             complement(1119..>2805)
                     /locus_tag="PLK08_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPS53707.1"
                     /translation="PVSARDTVNKLSLSGMADQVSCHASRQNGRLDFNVDMKLQDVSW
                     KYAEWPYAVEGASGDITITPDAVTLRNLQGHHGMGRITLDGDIKYAASRKLACELLVQ
                     GASVMFDGDFFAALPPAMRSRLEEFSLGGLADFRVKIKSDGTDAPVDFNVAIDASSMD
                     VCYKKIPIPLTQVSGRILIKPGRLELNNVQGVAGQDGDVMSVAASGYIENTPSSRQAN
                     LALNIVNVELDKTLMDSFSSETGNFLKRFRPGGRFSGKIRQFFYNKPKDKPAEWKITG
                     GFAFKDIEMDFGFGWRKLTGTWAGWLWREEKGMGMKAQCKVDRLKWDKYVASKLSGDL
                     TMQPGSDLIFINKLVGRSMGGRIAGKASLDIGTPLKYNMNLAMEDVDLAELFRSADGN
                     SPKDVSGKLRGEVEFSMIGGDKPMKQAAGEIEIYKASMYKMPVILGMLNVIYLTVPGD
                     GTFNSGFLRYHMKNEKLRLEEIFLTGSTLSVIGSGDLNLSDDTLNLTFLTGPPGKMPR
                     LNELATDLLSAISQELVEIRVSGTLKKPKMETVSFRSLETIIKRLVGTAAEQN"
BASE COUNT          765 a          668 c          588 g          784 t
ORIGIN      
        1 aggatgcttt tctacaattt tagccatgat ttcaagacaa tcatcacaat acaaaaaaat
       61 accctgctca tgcaacgcaa atacaggatg atgagttaag acaaatgaat cgaatttcat
      121 tagcaccttt tgtttgatta atagattcct taggggaaaa ctctagctat cagatttatg
      181 caaatacagc caataattca gaaactttat ttcgctgggt aactttactt gaaataaatc
      241 gccgaacatc aaaaaaatct atttgggcag acgaatacga attacgtgta aagtcagtat
      301 cgtgattcga aatgattaca gggatacctt tgtttgccaa tttttttgcc agtgcaacaa
      361 gtttatcttg ttcctgttgc ccgaatccac tgccagtgta atttgtaaaa tttgaagttt
      421 ccgtaagagg aacatagggt gggtcacaat aaatgacatc gcctacaact gctttcccca
      481 tagtttctac aaaatcagct gccatgaatt gtgctttttt gcttttttta acaaaatcca
      541 acatttcctt ttctggaaaa ggaggattct tgtagctccc gaatggaacg ttaaacagac
      601 ctcttgaatt atatctgcac aaaccgttaa agcaatggcg attcaaatat ataaatagtg
      661 cagcctttct ggttgaatca gatattgtat taaattcttc ccgcaatcgg taaaatactt
      721 tttctttgtt atttgcctta ataaataatt cgttacaata agcaataaaa tcagttccgt
      781 ctttttttaa ctgcttatat acattaatca aatcgatatt cgaatcagca atgagatatt
      841 tttcgtattt ggtatttaaa aaaacagcag atgagcctgc aaatggttca ataaggcgag
      901 tgccttgagg caaaacgcga ataattctgt caatgatttt atatttacca cctgcccact
      961 ttaaaaatgg tttcataaga gaattccgca taaaatactg attaaaatat aactacctat
     1021 acaacaagca ataaagtaaa aaacaatata caatatattg agagattaca attaagtttg
     1081 gtatatgtca agtatttaat aacaataaca actttaatct aattctgctc tgccgcagtg
     1141 ccgacgagac gttttattat ggtctcaagg ctgcgaaagc tgacggtttc cattttgggt
     1201 ttcttcagcg tcccggacac gcgtatttcc acgagttcct ggcttatcgc gctgagcagg
     1261 tcggttgcaa gttcgttcag ccgcggcatt ttgccgggag ggccggtcag aaacgtcaga
     1321 ttgagcgtgt cgtccgaaag attcaaatcg ccagaaccta tgaccgataa cgtactgccg
     1381 gtcaaaaaaa tctcttccag ccgcagtttc tcatttttca tatgataccg caaaaagccg
     1441 ctgttaaacg taccgtcgcc aggcactgtc aggtaaatca cattgagcat gccgagaatg
     1501 acgggcattt tatacatgct ggctttgtag atttcaattt cgccggcagc ctgcttcatg
     1561 ggcttgtcgc cgccaatcat gctgaattca acttcacccc tcaatttgcc agaaacgtct
     1621 ttgggcgagt tgccgtcagc cgaccggaac agttccgcca agtcgacgtc ttccatggca
     1681 agattcatgt tgtattttaa cggcgtgccg atatccaggc tggccttgcc ggcaattctt
     1741 ccgcccatcg aacggcctac aagtttgttt atgaatatca aatcactgcc cggctgcatg
     1801 gtaaggtcgc cggataattt gctcgcgacg tacttgtccc acttgagtct gtcgaccttg
     1861 cattgagcct tcatgcccat gcccttttct tcacgccaca gccagccggc ccatgtgccg
     1921 gtgagctttc gccagccaaa cccgaaatcc atctcgatgt ccttgaacgc aaatccgccg
     1981 gtaattttcc attcggccgg cttgtctttg ggcttattgt agaaaaactg tcgaattttg
     2041 ccgctgaacc ggccacccgg ccgaaatcgt ttcaaaaaat tgcccgtctc cgaagaaaag
     2101 ctgtccatga gagttttatc aagttcgacg ttaacgatat tgagggcaag gttggcctgg
     2161 cggctggagg gcgtattctc tatatatcca gatgccgcca cggacatgac atcgccatcc
     2221 tggccggcga caccctgaac attattcaat tccagccggc cgggcttgat aagtatccgg
     2281 ccgcttacct gagttaacgg aatcggaatt tttttatagc acacgtccat actgctggca
     2341 tctatggcaa cgttgaaatc caccggagca tccgtgccgt cggattttat cttcacgcgg
     2401 aagtccgcca gtccgccaag cgaaaattct tcaagcctgc tccgcatggc aggcggcaga
     2461 gcggcaaaaa aatcgccgtc aaacatcacg gatgccccct gaacgagcag ttcacaggca
     2521 agtttacggc tggcggcata tttaatatcg ccatccagcg ttatcctgcc catgccgtga
     2581 tggccctgca ggtttctgag cgtaacggca tccggcgtta tggtaatatc gccggatgca
     2641 ccttcgacgg cgtaaggcca ttctgcgtat ttccacgaaa catcctgcag tttcatgtcc
     2701 acattgaaat ccagcctgcc gttctgccgg ctggcgtggc aggaaacctg gtcagccatg
     2761 ccggaaagcg aaagtttatt gacagtgtcg cgggcagata ccggt
//
LOCUS       DAONFI010000315         2787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_46023_length_2787_cov_4.100659, whole genome shotgun sequence.
ACCESSION   DAONFI010000315 DAONFI010000000
VERSION     DAONFI010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46023_length_2787_cov_4.100659"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="PLK08_09160"
     CDS             <1..1470
                     /locus_tag="PLK08_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPS53708.1"
                     /translation="IISDDKWSNWWSRARSAVRKSANLTIEGKNPAVVVLHEQALTLE
                     DELAPARTAARAPKEFFDLIKTYYSEAAKRSVDVQSDFVDDIISMLAALATQFSQNCP
                     ADALEVTLMIDHAVRLGANAPTNCPTTQQLIEASQNAAEMIVQIKDVALQKDAFDALA
                     QRPDAADQFERLMLISPADLLDDIVSRLEKMGRNDAAERAIDVALNEPLDHTQIIIWL
                     WNKPGVEIANLPSHSTILIKLLEVLNDIDHRLVAPNEEKKARRQAIRAALSASKFKIY
                     NETIDAMDEAMASVIRTKIDRLHNLAITMYEAMMGKIRENHFALFVKKKIAPWLDENV
                     IWTTQAAYNARGEELNHIKEVLMPANAKQIGLAAEQGDLRENADWQAAIEERDMLVSR
                     VRKINSEIAMARVLERSDVPRDSVGIGSKIMIRRLDNAAETEMTFLGPWDSNPDEKIY
                     SYTTKLAQNLMGLAAGEKIELDFEGEVYEYEIVGIEAAI"
     gene            1497..>2787
                     /gene="argH"
                     /locus_tag="PLK08_09165"
     CDS             1497..>2787
                     /gene="argH"
                     /locus_tag="PLK08_09165"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPS53709.1"
                     /translation="MAKESKKSWQGRLKADADAATARLLASIDVDCRLWRYDIAGSIA
                     HAEMLCKEGIINKAEFARIKKGLLDIAADFEKNAITPDVSLEDIHMVIEAELIRRIGP
                     VGGKLHTGRSRNDQVALDLRLWARDAADNLIDAVRRVQKAFVSLAKKQGQLVMPAYTH
                     LQRAQPIVAGHSLLAYVEMLERDAERLADARRRINVSPLGSGAVAGTSIPLNREFTAA
                     ALGFENITRNSIDATSDRDFLAELCFDVALMGVHLSRWAEDWIIYSSGEFALISLHDA
                     YCTSSSMMPQKKNADTLELIRGKSAAGIAQLIGMLTLIKGLPLAYDRDMQDDKRFAFA
                     SVATMSDVLAVMPGLIESTTFCEKSITANIDKGFLDATSLAEYLVNKAVPFRTAHHIV
                     GRAVAAADAVGKSLAELSLAELQNFSPVFGDDVRNSLT"
BASE COUNT          711 a          696 c          763 g          617 t
ORIGIN      
        1 atcatatccg acgacaaatg gtccaactgg tggtcgcgtg cgcgcagtgc ggtacgcaaa
       61 tcggcgaatc tgacgataga aggtaaaaat cctgccgtcg ttgttcttca tgaacaggcc
      121 cttacgctgg aagatgagct tgcaccggct cgtacggcgg cgcgcgctcc taaggaattt
      181 tttgacctca tcaaaacata ttattctgaa gcggccaagc gttccgtgga cgttcagtct
      241 gattttgttg acgatataat ctcgatgttg gctgcacttg cgacacagtt ttcgcaaaac
      301 tgtccggccg acgcacttga agttactctg atgatagacc acgcagtcag gcttggcgcc
      361 aatgctccga caaattgccc gactacgcaa caacttatag aggcatccca aaatgctgcg
      421 gagatgatag ttcagataaa agacgtcgcc ttgcagaaag atgcctttga cgcacttgca
      481 caaaggcctg atgccgcgga ccagtttgag cgacttatgc ttatttcgcc ggcagacctt
      541 ctggatgata ttgtcagtcg ccttgaaaag atgggtcgta atgatgccgc cgagcgggct
      601 atcgatgttg ccctgaatga accgctcgac cacacgcaga taataatatg gctgtggaac
      661 aaacctggcg tggaaattgc gaatctgccg tcccattcga ctatactgat aaaactgctg
      721 gaagtgctta acgatatcga ccaccggctc gttgcgccga atgaggaaaa gaaggctcgt
      781 cgtcaggcta tacgcgcggc gctgtctgcg tccaaattca aaatatacaa tgagaccata
      841 gatgccatgg acgaagccat ggccagcgtc atccgtacaa aaatagatcg cctgcacaac
      901 ctggccatca ccatgtatga agccatgatg ggcaaaatcc gcgaaaatca ttttgctctt
      961 tttgtgaaaa agaaaattgc gccgtggctg gatgaaaacg ttatctggac cacgcaagcc
     1021 gcctataacg cacgcggcga agaactcaac catataaagg aagtgctcat gccggcgaac
     1081 gccaagcaga taggccttgc cgccgaacag ggtgaccttc gtgaaaatgc cgactggcag
     1141 gcagctatcg aagaacgcga catgcttgta tcgcgcgtgc ggaaaataaa ctctgaaatc
     1201 gccatggccc gcgtactcga acgttccgat gtgccgcgcg atagtgtcgg cataggctcg
     1261 aaaattatga tacggcggct tgataacgcg gctgaaaccg aaatgacttt cctgggcccg
     1321 tgggacagca atccggacga aaagatttat tcatacacga cgaaactggc tcagaaccta
     1381 atgggcctgg ctgccggcga aaaaatcgaa ctggacttcg aaggcgaagt gtatgaatac
     1441 gaaatcgtcg ggattgaagc tgcaatatga aaacatattt gcggagatga attgcgatgg
     1501 ccaaagaatc caaaaaatca tggcagggcc gcctgaaggc tgatgcagat gcggcaacgg
     1561 ctcggctgct ggcgagcata gacgttgact gtcggctttg gcggtatgac atcgccggct
     1621 cgattgcaca tgcggaaatg ctctgcaaag aaggtatcat aaacaaggcc gaattcgccc
     1681 gtattaaaaa aggcctgctc gacattgccg cagattttga aaaaaatgct ataacgcccg
     1741 atgtctcgct tgaagatatt catatggtaa tcgaggctga gcttatacgt cgcataggtc
     1801 ctgtcggcgg caagctgcat accggcagaa gccgcaacga tcaggtcgct ctcgacctgc
     1861 ggctgtgggc acgcgatgcc gccgataatc tcatcgatgc cgttcgtcgg gtgcagaagg
     1921 catttgtgtc acttgcaaaa aaacagggtc agcttgtcat gccggcgtat actcacctgc
     1981 agcgagcgca gccgatagta gccggccatt cattgcttgc atatgtggag atgcttgagc
     2041 gggacgccga gcggcttgcg gacgcacgca ggagaatcaa tgtttcgccg cttggcagcg
     2101 gagcggtcgc aggtacgagt atcccgctca accgcgaatt taccgctgcg gcactcggtt
     2161 tcgagaatat tacgagaaac agcatcgacg ccacgagcga ccgtgatttt ctcgcggaac
     2221 tctgtttcga cgtagccttg atgggagtgc atctgtcacg ctgggctgaa gactggataa
     2281 tttattcgtc cggcgagttt gcactcatca gcctgcacga tgcttattgt accagtagtt
     2341 cgatgatgcc gcagaagaaa aacgccgata cgcttgaatt gatacgcggc aaaagtgctg
     2401 ccggcattgc ccagcttatt ggcatgttga cgcttataaa aggtctgccg ctggcgtatg
     2461 accgtgatat gcaggacgac aaacgtttcg cgttcgcgtc ggtggcgaca atgtcggacg
     2521 tacttgccgt catgcctggc ctgattgaaa gcaccacatt ttgcgaaaag tccataacgg
     2581 ccaacattga caagggtttt cttgacgcaa cgagtcttgc ggaatatctt gtgaacaagg
     2641 ctgtgccgtt ccggacggcg catcatatcg tgggcagggc ggtggcggcg gctgacgctg
     2701 tcggcaaatc gctggcggag ctttcgcttg cggaattgca gaatttttca cccgtcttcg
     2761 gtgatgacgt cagaaacagt ctcacgg
//
LOCUS       DAONFI010000316         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_47459_length_2731_cov_4.277280, whole genome shotgun sequence.
ACCESSION   DAONFI010000316 DAONFI010000000
VERSION     DAONFI010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47459_length_2731_cov_4.277280"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLK08_09170"
     CDS             complement(<1..1387)
                     /locus_tag="PLK08_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPS53710.1"
                     /translation="MTRKMEMMDGNQAAAHVAHRVSEVIAIYPITPSSPMGEFSDSWS
                     SLGQKNLWGTVPLVAEMEHEGGAVAASHGALQTGSLTTTFTASQGLLLMIPTLYKLAG
                     ELTSTCFHVTARSLACQALSIFGDHGDVMAVRQTGVAMLVSNSVQEAHDMALIGTAAT
                     LKSRLPFIHFFDGFRTSHEIQKIECLNDEDIRSMIDDDLVLAHRARGLSPDKPVVRGT
                     SQNPDVYFQGRETVNRYYNACPAIVQQYMDDFAKITGRQYHLFDYVGAKDADRVIILM
                     GSGAEAADEAVESLLAGGEKVGIVKVHLYRPFCNEAILAAIPASCRKIAVLDRTKEPG
                     SAGDPLYLDVVNALVEGGRTDIKVVGGRYGLSSKEFTPAMVKGIYVELGKAQPKNHFT
                     VGIVDDVTNTSIDYDKNFHTEQSGMVQAMFYGLGSDGTVGANKNSIKIIGSETDNYAQ
                     GYFVYDSKKAGA"
     gene            complement(1746..2459)
                     /locus_tag="PLK08_09175"
     CDS             complement(1746..2459)
                     /locus_tag="PLK08_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53711.1"
                     /translation="MSIKICRAAIKILSLSAICVFLSFGACTMCGCEEQDATISAASN
                     SKVMPSANEARQAMDTISQVKRTSQTLQAKQESLMKLMIFTLSIIGIALPLLFWLLQR
                     KFMAEQEQRLARRLEQREIDVKNEIVDLERKMMDVTQQQRSQLIAELEQLRQKSLELD
                     RTTREMFSEALHNFKNTRILKKPGFIARLFGFGKNRMNIPENTTIVQINRPQNESQSF
                     FGKDSSSSSSQNFSDKADN"
     gene            complement(2508..>2731)
                     /locus_tag="PLK08_09180"
     CDS             complement(2508..>2731)
                     /locus_tag="PLK08_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HPS53712.1"
                     /translation="LDTAGVPDPDLVIRTSGEMRLSNYLLWQLSYAEFYITPEHWPDF
                     NEAEFQKALIAYSRRNRRFGGLTQMLPDS"
BASE COUNT          565 a          707 c          753 g          706 t
ORIGIN      
        1 tggcgccggc tttcttcgag tcgtacacga aatagccctg cgcgtagttg tcggtttcac
       61 tgccgatgat ttttatggag tttttgttcg caccgaccgt gccgtcactg ccgaggccgt
      121 agaacatcgc ctgaaccatg ccggactgct cggtgtggaa atttttatcg tagtcgatgc
      181 tggtattggt cacgtcgtcg actatgccga ctgtgaaatg gtttttcggc tgggctttgc
      241 cgagttcgac atagatgccc ttgaccatgg caggggtgaa ttccttgctg ctcaggccat
      301 agcggccgcc gacgactttt atgtcggttc tgccgccttc gacgagggcg ttgacgacgt
      361 ccagatacag cgggtcgccg gctgagccgg gctctttcgt ccggtcgaga actgcgattt
      421 tcctgcagct tgccggaatc gccgcgagaa tagcctcatt gcagaacggc cgataaaggt
      481 gtaccttgac aatgccgact ttttcgccgc cggcaaggag cgattcaacc gcttcgtcag
      541 ccgcctcggc gcctgaaccc atgaggatta ttacgcggtc ggcatctttc gcgccgacgt
      601 aatcgaagag atggtactgg cggccggtaa ttttggcaaa atcgtccata tactgctgaa
      661 cgattgcagg gcaggcgttg tagtatctgt taacggtttc acggccctgg aagtagacgt
      721 cgggattctg gcttgtgccg cgaacgaccg gcttgtcggg cgacaacccg cgggcacgat
      781 gcgccaatac aaggtcgtcg tctatcatcg agcgaatgtc ctcgtcattg aggcattcga
      841 ttttctgtat ttcatgcgag gtgcggaaac cgtcgaagaa gtggatgaag ggcagtcggc
      901 tcttgagagt ggccgccgtg ccgatgagcg ccatgtcgtg tgcttcctga acgctgttcg
      961 agacgagcat tgccacgcct gtctggcgga cagccatcac gtcgccgtgg tcgccgaaga
     1021 tgctcaatgc ctggcaggct agcgaacgag ccgttacatg aaagcatgtg ctggtgagtt
     1081 cgccggcaag tttgtatagt gtgggaatca tcagcagaag gccttgcgac gcggtgaatg
     1141 tcgtcgtaag cgagccggtc tgcaacgcac cgtgcgacgc ggcgactgct ccgccttcat
     1201 gctccatttc cgctacgagc ggtactgtcc cccaaagatt cttctggccg agggagctcc
     1261 aactatcgga gaactcgccc atcgggctgg atggcgtaat cgggtagata gcgataactt
     1321 cgctcaccct gtgggcgacg tgtgcggcgg cctgattgcc gtccatcatt tccattttac
     1381 gtgtcatgac gtttcctcaa aaaaatattg tgtagaacaa ccatgcccct gcgcctggtg
     1441 tggacgccga gcaacataat ttctgcccct gcgcctggtg tggacgccga acagatgccc
     1501 tttccgcgaa tgcagaaact ggaacttgtc attctcgcaa attttgcgtc tgaattcaac
     1561 gcttttcgga ggctgtgtaa aaatagctga gtggaaatat cggcatggag tggttcgcga
     1621 atttttttgc ttttagaact ttcaaaatat gactgcattc ttcatgggtt aaaaacgcgg
     1681 cggcaaagtt tttacacttc aacaatttac gacgtactca aagcgctgtc cggctgacgt
     1741 gattttcagt tgtcagcctt gtcggaaaaa ttttgtgaag aagatgatga tgagtctttc
     1801 ccgaaaaacg actgcgattc attctgcggc ctgtttatct gcactatcgt cgtgttttcc
     1861 ggaatattca tgcgattttt accgaatccg aaaagccgcg cgatgaaccc cggcttttta
     1921 agtatccgcg tattcttgaa attatgaagc gcctcgctga acatttcccg cgtagtacgg
     1981 tcgagttcga gcgatttctg acgcagctgt tcaagctcgg cgataagctg cgaacgctgt
     2041 tgctgagtca catccatcat tttccgttcg aggtcgacga tttcgttttt gacgtcgatt
     2101 tcgcgctgtt caagacgcct ggccagccgc tgttcctgct cggccatgaa ttttcgctgc
     2161 aacagccaga acagcagcgg cagggcgatg ccgataattg acagcgtgaa aatcatcagt
     2221 ttcattaggc tttcctgctt ggcctgaagc gtctggctgg ttcgcttgac ctgcgagatg
     2281 gtgtccatcg cctgccgggc ctcgttggct gagggcatga ctttcgagtt agacgccgcc
     2341 gatatggttg cgtcctgctc ttcgcagccg cacatcgtac atgcgccgaa actcaaaaaa
     2401 acgcatatcg ccgacaacga cagaatttta atcgcggctc tgcaaatttt tatggacatt
     2461 gtgtcatctc ctttgcgaag acattttgca ctttcatcgc catgatttca agagtccggc
     2521 agcatttgcg tcaagccgcc gaatcgtctg ttgcgacggc tgtatgcgat gagggctttt
     2581 tggaattcgg cttcgttgaa atcgggccaa tgttccggcg taatatagaa ctccgcatac
     2641 gaaagctgcc agagaagata attgctcagc cgcatttcgc cggatgtacg tattacgagg
     2701 tcaggatccg gtacgccggc tgtgtccagc g
//
LOCUS       DAONFI010000317         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_47894_length_2714_cov_3.300489, whole genome shotgun sequence.
ACCESSION   DAONFI010000317 DAONFI010000000
VERSION     DAONFI010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47894_length_2714_cov_3.300489"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1097
                     /locus_tag="PLK08_09185"
     CDS             186..1097
                     /locus_tag="PLK08_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPS53713.1"
                     /translation="MNLTVLKTFCDLVDSGSFSRAAESNYVSQSAVSQQLSKLESTLS
                     VQLISRGGGIVVPTEAGKAFYRGAKEICRRYEQLIGEVRSVSDSVRGTLRVGTIYSVG
                     FYLLDPYVREFFQKHSEANLYVSYTSWNQIYASVIDGEMDMGIVACPDANRSLDIIPL
                     EDEQLVVVCPPGDPLAHRESVVASDLEGRKFAAFSGNIPTRRHIDKLLKAAKVRVNIT
                     LEFDNIELLKRVIMIGSAVSILPEESVRSEALRGDLAFVPFRYPKRWTRPVGIVRRKD
                     RQPTPAEVQFLTILAPKTKKKFFKVEE"
     gene            1136..1984
                     /locus_tag="PLK08_09190"
     CDS             1136..1984
                     /locus_tag="PLK08_09190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPS53714.1"
                     /translation="MSYEIKRMSLLDVEEAIEWAAAEGWNPGLGDAPVFYSADSRGFF
                     MAFDGSQPVGVISAVAYDDKFGFIGFFIVRPDLRGHLIGAKLGQTALEYLGDRNIGVD
                     GVEDKMEKYTHFGFMLAYHNYRYGGRTRSVRKLPDGVTQFDEPSDFVDILAYDRLCFP
                     AQREKFMRGWLGMAGSYSVVASDKNGRLQGFATARKCRVGYKIAPLFADDVRIAEKIL
                     AGLQCLMPAESAMFLDIPGPNKQAIELARSLDMECVFGTARMYSKCQPDIDVNRIFGV
                     TSFELG"
     gene            complement(2030..>2714)
                     /locus_tag="PLK08_09195"
     CDS             complement(2030..>2714)
                     /locus_tag="PLK08_09195"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HPS53715.1"
                     /translation="SRGGKVIIPSFSVGRTQNVVYYLVNAINEGILEPLPIFVDSPLS
                     TNATAVFKNHPECFDEAAKDFWLQQGDLFGGKLVQYITDVNASKALNSLKEPHVIIAA
                     AGMCESGRILHHLKNNVEDENNSICIVGFMAQHTLGRRIVERRDEIKIFGKMYRLLAR
                     VEILNGFSSHADTNDFRLLLGPLAKNLKGAFVVHGEDSQLTAMKDLLESYGCRNVNIP
                     NPGDIFTLE"
BASE COUNT          641 a          631 c          748 g          694 t
ORIGIN      
        1 gctatctgct tcgccttcct tgccagccaa tcaaccaact acgcctcggt gcgacgtctc
       61 attttattag aagctcttat aaattccatt atgcctttta gggcattttt tttgagatgt
      121 tgcattagaa aaattaaagt cgctattcaa tatatctaat tattggcgat tttcaggagc
      181 gtataatgaa ccttaccgtg ctcaagacat tctgcgacct cgtcgattcc ggcagttttt
      241 cccgcgccgc cgagtcgaat tacgtgtccc agtcggctgt ttcgcagcag ttatccaagc
      301 tggagtcgac gttgtcggtg caattaatat cacgtggcgg aggcatagta gtgccgacgg
      361 aggcgggcaa ggcattttat cgcggtgcaa aggaaatatg ccgccgctat gaacagctca
      421 tcggcgaagt tcgcagtgtc agcgattccg tccgcggcac attgcgggtc ggcacgatat
      481 attccgtagg cttttatctg ctcgaccctt acgtgcgtga atttttccaa aaacattcgg
      541 aagcgaacct gtacgtctcg tataccagct ggaatcagat ttatgcgtcg gtcatcgacg
      601 gcgagatgga tatgggtata gtggcttgtc cggatgccaa ccgctcgctt gatatcatac
      661 cgctggagga tgaacagctg gttgtcgtct gcccgcctgg cgatccgctg gcacataggg
      721 aaagcgtggt ggcgagtgac ctggagggca gaaaatttgc cgcgttttcc ggcaacatac
      781 cgacgcggcg gcatatcgat aagctcctga aagccgccaa agtacgcgtc aacataacgc
      841 tggaattcga taatattgaa ctgctgaaac gtgtgataat gattggctca gccgtgagca
      901 tattgcccga agaaagcgtt cgcagcgagg cgttgcgcgg cgatcttgcg tttgtgccgt
      961 tccgatatcc caaacggtgg accaggccgg tcggcatcgt ccgccgcaaa gaccgtcaac
     1021 ctacgccggc agaagtgcag ttcctgacca tactcgctcc gaaaacgaaa aagaaatttt
     1081 ttaaagtcga agaataaaaa gcggctgacg gaatttttgt gtatacaatt aaagcatgag
     1141 ttacgaaata aaacggatgt cgctgctcga cgtcgaagag gcgatagagt gggcggcggc
     1201 ggaggggtgg aatccaggct tgggtgatgc gccggttttt tattcagccg attcgcgggg
     1261 atttttcatg gcgtttgacg gcagtcagcc ggtgggagtg atatcggcgg tggcgtatga
     1321 cgacaaattc ggatttatcg gttttttcat agttcggccg gacttgcggg gacacctcat
     1381 aggcgccaaa cttggacaga ctgcgctgga gtatcttggc gacagaaata tcggcgtgga
     1441 cggcgttgaa gacaagatgg aaaaatatac gcatttcgga tttatgctgg catatcacaa
     1501 ttatcgctac ggcggcagga ctagaagtgt ccgtaaattg ccggacggtg tgacgcagtt
     1561 tgacgagccg tctgattttg ttgatattct tgcgtatgac aggctgtgtt ttccggcgca
     1621 gcgggaaaaa ttcatgcgtg gctggctggg catggctggt tcatatagcg tagttgccag
     1681 cgataagaac ggacggctgc agggattcgc aacagccaga aaatgcagag ttggatataa
     1741 aatcgcaccg ctatttgcag atgacgtgcg gattgcggaa aaaatcctgg ctggcttgca
     1801 atgtctcatg ccggcggaga gtgcgatgtt tctggatatt cctggcccga ataaacaggc
     1861 tatcgaactc gcaaggtcgc tagatatgga atgcgttttc ggcacggcaa ggatgtactc
     1921 gaaatgtcag ccggatatcg acgtcaatcg tatcttcggc gtaacgtcgt tcgaactggg
     1981 ataaattttt ggacgttaag catcctgaag ttgacatgca gtcatcctgc tattccagtg
     2041 taaaaatatc gccggggttg gggatattca cattgcgaca tccgtaggat tccaataaat
     2101 ctttcatcgc cgtcaactgc gaatcttcac cgtgaaccac gaacgcgcct ttcaggtttt
     2161 tcgccagcgg cccaagcagc agccggaaat cgtttgtgtc ggcatgcgag ctgaatccgt
     2221 tcagaatttc aactctggcc aacaggcgat acattttgcc gaagattttt atttcgtctc
     2281 gacgttccac tattcgccgg cccagcgtgt gttgcgccat gaagccgacg atgcagatgg
     2341 agttattctc gtcttccacg ttatttttga ggtgatgaag aatacggccg gattcgcaca
     2401 tgccggctgc cgcgattatg acgtgcggtt cttttagaga attcaacgcc tttgacgcat
     2461 ttacgtccgt gatgtactgc actagtttgc cgccgaacag gtcgccctgt tgcagccaga
     2521 aatccttggc tgcttcgtcg aaacattccg gatgattttt aaataccgcg gtagcattgg
     2581 tggaaagcgg agaatcaacg aatatcggca gcggttcgag tatgccttcg tttatcgcgt
     2641 tgacgagata gtagacaaca ttttgcgttc tgccgacgct gaaagacgga atgatgacct
     2701 tgccgccgcg gctg
//
LOCUS       DAONFI010000318         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_48186_length_2702_cov_4.218738, whole genome shotgun sequence.
ACCESSION   DAONFI010000318 DAONFI010000000
VERSION     DAONFI010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48186_length_2702_cov_4.218738"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..801)
                     /gene="deoC"
                     /locus_tag="PLK08_09200"
     CDS             complement(121..801)
                     /gene="deoC"
                     /locus_tag="PLK08_09200"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPS53716.1"
                     /translation="MLPINRYIDHAVLKPELTRDETVRQIESGLQFNVRTVCVKPADI
                     LIAQKILDSSQTEVSCVLSFPHGNSLPASKSDEASRYIDLGVAEIDMVVNYGLIRSGL
                     WDELRKDILAVTTQTKLAGVKVKTILETACLTAEQIDTATKIAVECNADFVKTSTGFG
                     PGGATEEAVRVMLKAADSRIGVKASGGIHDFSTAATYLKMGVTRLGIGAATTPVICNA
                     ACADVENF"
     gene            complement(1029..1907)
                     /gene="rsmA"
                     /locus_tag="PLK08_09205"
     CDS             complement(1029..1907)
                     /gene="rsmA"
                     /locus_tag="PLK08_09205"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPS53717.1"
                     /translation="MQPVKKIQQLLDAHDARPMGRFGQCFLIDLNYMAKVLDIAAVDA
                     KQCTLEVGPGTGSLTEELLKKSAKVVAAEIDRKFCEILREIFGERENFTLIEGDALDG
                     KHAIAEKILHAVSPRCSLVANLPYNAATSLVIDALIESCLAVSQPANHVTFDSLTFTV
                     QKEVADRLAAVHGDDYGLVSVIVALMSDIKLGPVIPPSAFWPQPKIDSRIVRIDFNPQ
                     KAVKIKSLDTLRKLLHMAFTQRRKHLLAAAKAKNSPFTPQQLATALLQLNMPQTMRCG
                     ETSPEFFLNLSNILAD"
     gene            complement(1909..2607)
                     /locus_tag="PLK08_09210"
     CDS             complement(1909..2607)
                     /locus_tag="PLK08_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53718.1"
                     /translation="MPKTTFYILAVVAAGLCGCVPQHVKPMEPEPATQAERNFNALWE
                     SSRELLKKYDYEIDRMDKREGIITTYATTGGQFFEVWRKDSGNFFDWRENSVKTMLRA
                     VRITIFRVGDSDRYDFQLEVRMARTDLPNAVLSDTSQTNNIIENGGPAAKVHQVEYYR
                     RKDAFDHLTFEDLSRVPDKSESHDLPAMKTSNAASIIIPMGRDADLEAKMTTRLKDAV
                     ETYSPRRAGTWVSF"
BASE COUNT          594 a          634 c          716 g          758 t
ORIGIN      
        1 aatacgcgtt tgtcgagcag gacggcaact gggctgcgtc gcctatggac tcggcgaaat
       61 taagcctgaa atatctgcaa tcctgaatat atctgagcag tgtagcggaa caatcatcta
      121 tcaaaaattt tcgacgtctg cgcaggcggc attacatatt accggtgtcg tcgcggcacc
      181 gatgcccagg cgagtgacgc ccatttttag atatgtcgcc gcggtagaaa agtcgtggat
      241 tccgccggac gcttttacgc cgattcggct gtcggcggct ttgagcatta cgcggacagc
      301 ctcttcagtg gccccgccag ggccgaaacc cgttgaagtt ttcacgaaat cagcattgca
      361 ctccacggcg attttcgtag ccgtatctat ctgctcagcc gtcaggcagg cggtttccag
      421 aatggttttc acttttacgc cagccagttt tgtctgagtt gttacggcca atatatcttt
      481 gcggagttca tcccacaggc cgctacgaat caaaccgtaa ttcacgacca tgtcgatttc
      541 cgctacgccc aggtcgatat aacggctggc ttcgtcggat ttcgacgcgg gaagtgagtt
      601 cccgtgcgga aaagacaaca cgcatgatac ttcagtctga ctgctgtcga gaattttttg
      661 ggcaattaat atatccgccg gtttgacgca gaccgtgcgg acgttaaatt gcaggcctga
      721 ttctatctgg cggacagtct cgtcgcgggt cagttccggt ttcaacactg cgtggtcaat
      781 atagcgattt attggtaaca tttctttgtt aaaatttata aaaatccgaa gcaactcatg
      841 gatattgaaa ttcaaaattt acagatagaa ttttaagaca ccgtcttctt gaattcaaat
      901 gccaatttat gatttgaaat tttttgtaaa gaaataacgc gataatgcag ccaaagaacc
      961 gctttgacag gcattgccgc ttccggccac tatattcgtc gttggcgttc catcgtatgg
     1021 ttgctgcatc agtcagccag aatatttgaa agattcaaaa aaaattccgg cgatgtttcg
     1081 ccgcatcgca tggtttgggg catattcagt tgcagcaggg cggttgcgag ctgttggggg
     1141 gtgaacggag aattttttgc tttagccgca gccagcagat gtttgcgacg ttgggtgaag
     1201 gccatgtgca gaagttttcg caacgtatcg agagatttga tttttacggc tttttgtgga
     1261 ttaaaatcta tgcgaactat ccttgaatct attttaggtt gcggccagaa tgcgcttggc
     1321 ggtatgacgg gcccgagttt tatatccgac ataagagcca cgatgacgct gaccagtccg
     1381 taatcgtcgc cgtgtaccgc tgccagccgg tcggcgactt ctttttgtac agtgaatgtc
     1441 aggctgtcga acgtaacgtg gtttgccggc tgcgagacgg caaggcagga ttcgatgagt
     1501 gcgtcgatta ccagtgacgt tgccgcattg tacggcagat tagccacgag cgagcatcgc
     1561 ggcgagacgg catgcagtat cttttcagcg atggcatgtt taccgtccag cgcgtcgccc
     1621 tctatgagcg taaaattctc gcgttcgcca aaaatttctc gcaggatttc gcaaaatttg
     1681 cggtctattt ctgccgctac cacttttgcc gattttttca gcagttcttc agtgagtgaa
     1741 ccggttcctg gcccgacttc aagcgtacat tgtttagcgt cgacggctgc gatatccaga
     1801 accttggcca tgtagttgag gtctatcagg aagcactggc cgaagcggcc cataggcctg
     1861 gcgtcgtgtg cgtcgaggag ttgctgaatt ttctttaccg gctgcatttc agaatgaaac
     1921 ccatgtgccg gcgcggcggg gcgaatatgt ttcgacggcg tctttgaggc gcgtcgtcat
     1981 tttggcttcc aggtcggcat cgcggcccat tggaataata atcgaagcgg cgtttgatgt
     2041 tttcatcgcc ggcaggtcat gcgattcaga cttgtcggga acgcggctga gatcttcgaa
     2101 cgtcaggtgg tcgaacgcgt ctttgcggcg ataatattcg acttgatgta cctttgcggc
     2161 tgggccgccg ttttctatta tgttatttgt ctggctggta tcggataaca cggcgttggg
     2221 caaatcggtt ctggccatcc gcacttcaag ctggaaatca tacctgtcgg aatcgccgac
     2281 gcggaaaatg gttatcctga ctgcgcggag cattgtcttt acggaatttt cacgccagtc
     2341 gaaaaaattg cccgaatctt tccgccagac ttcaaaaaac tgtccgccgg ttgtggcgta
     2401 tgtggttatg atgccttcac gtttatccat gcggtctatt tcgtagtcgt attttttcag
     2461 caattcacgg ctggattccc agagggcgtt gaaatttctt tctgcctgcg tggccggctc
     2521 gggttccatt ggtttgacgt gttgcggcac gcagccgcat aagccggcgg ccacaacagc
     2581 cagaatgtaa aatgtcgttt ttggcatgga aataggataa actattcttc tcaaagtgca
     2641 aagttttttc tttttatcgg ctgggctgtt tttggaagat tagctgttgc atgggcttat
     2701 cg
//
LOCUS       DAONFI010000319         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_48555_length_2689_cov_4.555429, whole genome shotgun sequence.
ACCESSION   DAONFI010000319 DAONFI010000000
VERSION     DAONFI010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48555_length_2689_cov_4.555429"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLK08_09215"
     CDS             <1..357
                     /locus_tag="PLK08_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPS53719.1"
                     /translation="IETNGSMNLEGVDGRVRKIMDIKCPLSGQADSFFPSNVKQLTRT
                     DEVKFVVAGREDFDYAAGIINKYDLCSICEVVFSPVMPDMPPRDLAEWIIKSGLDVKL
                     GLQLHKIIWPDIQRGV"
     gene            361..723
                     /gene="queD"
                     /locus_tag="PLK08_09220"
     CDS             361..723
                     /gene="queD"
                     /locus_tag="PLK08_09220"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPS53720.1"
                     /translation="MEVELVKTFEFAAAHSLDRAPDGHKCRNFHGHSYRVDVHVTGPV
                     NPQRGWVIDFGMLKELVQPLIDRLDHSNLNEVEGLDNTTSEMLAKYLWDRIKPAMPLL
                     SAITVWESATSRCTYRGE"
     gene            complement(1073..1432)
                     /locus_tag="PLK08_09225"
     CDS             complement(1073..1432)
                     /locus_tag="PLK08_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53721.1"
                     /translation="MKAVRMILVASLILGFTTCAVQAEGNGNKKAKCKMVAGTVSAVS
                     ATSITIKPKKKDAEDVTIAINDETKIKVNGKEAAASDIKEGMKATIKHIDGTATQINA
                     NDKKDKTKKKEKPEPKE"
     gene            1715..1906
                     /locus_tag="PLK08_09230"
     CDS             1715..1906
                     /locus_tag="PLK08_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53722.1"
                     /translation="MKDKKRRISESESDAFFEVDEGVWDDDGNDGRKKRDSGKRRHRR
                     DYGDSGDSGDSRSSRGFNA"
     gene            complement(1947..>2689)
                     /locus_tag="PLK08_09235"
     CDS             complement(1947..>2689)
                     /locus_tag="PLK08_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HPS53723.1"
                     /translation="KLCEFIDCCDAFAGVRLEGIQFHTSWNMNPSAQVAFLSVLGDEL
                     KKLSATHRNRIKFLDIGGGFWPEQGEWLTPEGTALGRVLKELAPELLADDRRRFRIPA
                     VPIEDFAGAITTGLREYVFPYVECGIYAEPGRWLINSGMHILIKVLDVKDGGNMVITC
                     GGINAVGWERFETDYCPVLNLSSPAPNRQYCELAGSLCTPHDIWGREFWGKSIAKGDI
                     LLIPEQGAYTYSLKQEFIKPLPACVVTE"
BASE COUNT          654 a          628 c          661 g          746 t
ORIGIN      
        1 atcgagacca acggcagcat gaacctggaa ggcgttgacg gacgggtacg gaaaatcatg
       61 gatataaaat gtcccctgag cggccaggct gattcatttt ttccgtcgaa cgtcaaacag
      121 cttacgcgga ccgacgaagt caaattcgtt gttgccggcc gtgaagattt tgattatgcc
      181 gccggtatta taaataaata cgacctatgc tcgatatgcg aggttgtatt ttcaccggtc
      241 atgccggata tgccgccgcg tgacctggca gaatggataa taaaaagcgg tctcgacgta
      301 aaactcggcc tgcaactgca taaaataatc tggcccgaca tacagcgtgg agtgtgaatc
      361 atggaagttg aacttgtaaa gacttttgag tttgcggcgg ctcattcgct tgacagggcg
      421 cctgatggcc ataaatgtcg taattttcac ggccattcgt acagggttga cgtgcacgtg
      481 acagggccgg tcaatccgca gcgcggctgg gtgatagatt tcggcatgct caaggagctc
      541 gttcagccgc tgatagaccg gctggatcat tccaatctca atgaagtgga aggccttgat
      601 aataccacaa gcgaaatgct cgccaaatat ctatgggata gaatcaaacc cgcaatgccg
      661 ctgctttcgg cgataacggt gtgggaatct gctacgtcca gatgcactta ccgcggcgaa
      721 tgatttcaga acctatccca aaaactcact gaccgttctg atgccgtttc agatttgcgt
      781 gctgcccggg aggataccaa actctgtcca cggcattcat ttacacttca aaatagcgat
      841 aaaaaaagtg cggctatcat gtagttggca ataatagtcg cactttaaaa atttcttcaa
      901 caactaactc aaagtgattg atttcagatt atcatgctcg ataaagaact atcttgcata
      961 cagtctgtaa ttttaaacaa tcgtcgcgct gctggctgat tgagcagatt ccattgcaga
     1021 atagcgttgt gtgtcatggc gttgaaaatt tgaatcagcc agtttaaaac aatcattctt
     1081 tgggttcagg cttttccttc tttttggttt tgtctttctt gtcatttgca tttatttgcg
     1141 tggcagttcc gtcgatatgt ttgatggtgg ctttcatgcc ttccttgata tcgctagctg
     1201 cagcctcttt gccgttaact ttaattttcg tttcatcatt tatggcaatg gttacgtctt
     1261 cggcatcttt ctttttgggt ttgattgtaa tagaagttgc gctgacagcc gaaactgtcc
     1321 cggcgaccat tttgcatttt gcctttttgt ttccgttgcc ttcagcctgg acggcacacg
     1381 tcgtgaaacc caatatcagg ctcgccacga gaatcattct tacagctttc atttctacat
     1441 ctcctgaaaa taccatattt gcgccggtac acccgacgta attgctttga tgataaataa
     1501 aacgcacggc aatccagaat ataaaaaagc attcccgaaa ttatacctcg cggcaggcta
     1561 ggaaacaaat aatttgctga ctcctgcgtt atgccgtaag gccagccgag tgaaaaattc
     1621 cgttgttttt cgccgattct ggtataaaat atatgttcat acccggcgtt tgagcatatg
     1681 tttattttcg tgcaatgcaa ttgaggatat ccgtatgaag gacaagaaac gcagaatatc
     1741 cgagtcggaa tcggatgcat tttttgaagt tgacgaaggc gtatgggatg atgatggcaa
     1801 cgacggccgt aaaaaacgcg acagcggcaa gcgtcgtcat cgccgtgatt atggagattc
     1861 gggtgacagt ggcgattcgc gttccagccg cggttttaat gcataattaa agttgggtag
     1921 tgggtcaatt cgaaataaag tgacggttat tccgttacga cgcaggctgg cagcggtttt
     1981 atgaattcct gtttgaggct gtatgtatat gcgccttgtt cagggataag cagaatatcg
     2041 ccttttgcta tggactttcc ccaaaattct ctgccccaga tgtcatgcgg cgtacagagt
     2101 gagcctgcga gttcgcaata ctgtcggttt ggggccggac tgctcaggtt cagtacgggg
     2161 cagtaatcgg tttcgaatct ttcccagccg accgcgttga tgccgccgca ggttataacc
     2221 atattgccgc cgtcttttac gtccagcact tttatgagta tatgcatgcc gctgtttatc
     2281 agccagcggc ccggctctgc gtaaatgccg cactccacgt aaggaaagac gtattctcgc
     2341 aagccggtcg ttattgcgcc tgcaaaatcc tctattggca cggctggaat cctgaaacgg
     2401 cgacggtcgt cggccagcag ttccggtgca agttccttga gcactctgcc gagggctgtg
     2461 ccttcgggtg tcagccattc gccctgttca ggccagaagc cgccgcctat atccagaaac
     2521 tttatcctgt ttctgtgagt ggcggagagt tttttgagtt catcgccgag taccgacagg
     2581 aacgccacct gtgccgacgg attcatattc cagctcgtgt gaaactgtat gccttccagc
     2641 cgtacgccgg caaaagcgtc gcaacagtcg atgaactcgc agagttttg
//
LOCUS       DAONFI010000320         2680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_48807_length_2680_cov_4.188952, whole genome shotgun sequence.
ACCESSION   DAONFI010000320 DAONFI010000000
VERSION     DAONFI010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48807_length_2680_cov_4.188952"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLK08_09240"
     CDS             complement(<1..337)
                     /locus_tag="PLK08_09240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS53724.1"
                     /translation="MRIALVIERLDSRRGGRELSTVELAAALAEAGQDVTVLTMEAAD
                     HEILGRVHVDVLAACGAGKAERFGHFAAAVAERKKDFDIVHGMTAMPCLDVYQPRGGS
                     YREAMLQNAE"
     gene            complement(334..1155)
                     /locus_tag="PLK08_09245"
     CDS             complement(334..1155)
                     /locus_tag="PLK08_09245"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPS53725.1"
                     /translation="MKESPLAPAIVDFIRKHRLDTLDGAFSFSDGSDMVKPGLGRRRR
                     TRVEFHDDDGRGWILFIKRYEAPSFWRRLCRLAAFRRHWCEAKNEFDNICAVRQADVP
                     TMRAVICGAHLGFWGVGRNFIVVTAVPGQSLEKSGDAFFEKYQQNHDMLDSFNRQILR
                     LVKTFHAAGFVHRDLYASHIFMHEHDGLLELNLIDMARVFRPVRRKFRWRVKDLAQLK
                     YSMPAFWVEKYFNRFLEAYLGTSVPKVMKRWQRAIDRRVASMARRAERKRTESVK"
     gene            complement(1152..2252)
                     /gene="waaF"
                     /locus_tag="PLK08_09250"
     CDS             complement(1152..2252)
                     /gene="waaF"
                     /locus_tag="PLK08_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPS53726.1"
                     /translation="MTGRTINRIMVFLPNWVGDVVMATPVLREIRQKNFPDAKVAFFG
                     RAVSLAVMAGSSWADVIIEDTSRKKPRLRNFLSTVRAVRQFAPDVTLLLPNSFRSALI
                     ARLGRCGRIIGYARSGRTLLLDEKIMPVFSADSPTGFKPMPMLDYYSRLLEPLGMNCV
                     NRTMELPVTPDGEEQACRLLAECGYDAARPLVMLNPGASFGTSKLWPAERFAALADAL
                     ADEFSAQIIINAAPNPNEQAIAAKVSASMKTRPLVDFASRNNSIASLKSLVKRCALMV
                     TNDTGTRHIAAAFGCGVVTIFGSTDPVWAQIDYPRERIVRVDVPCGPCQKKFCPLPAG
                     PSHHRCVTGVSVDMVLAACRELLGSESVEKRL"
     gene            complement(2249..>2680)
                     /locus_tag="PLK08_09255"
     CDS             complement(2249..>2680)
                     /locus_tag="PLK08_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53727.1"
                     /translation="EAAAALRSISRRANIIYLTRRDSTTQNNNHEWFQRAGLPDGPIL
                     LWQKQRWHIVREGRFNLPRIVIESRLDSQLDSLKTVFPNFRVGICQSSLAGKAFREAG
                     LMPIFVGKTPPLEFRRSEIRTDWKDLEARGLDGMLSSDEGK"
BASE COUNT          614 a          802 c          680 g          584 t
ORIGIN      
        1 tttcggcatt ttgcagcatg gcctcccggt aacttccgcc gcgcggctga tagacgtcca
       61 ggcacggcat agccgtcatg ccgtgaacaa tgtcgaaatc ttttttacgt tcagcgactg
      121 cggcagcgaa atgtccgaat cgttcggctt tgccggcgcc gcaggctgca agcacatcga
      181 catgaacccg tcccaaaatt tcatggtcag cggcttccat agtcagcacg gtaacatcct
      241 gtcccgcctc tgcaagcgca gccgccaatt ccacggttga aagctccctt ccgccgcgac
      301 gcgaatccag ccgttctatc acgagcgcaa ttctcatttg acgctttccg tacgttttct
      361 ttctgcgcgc ctggccatgg acgcaacacg gcggtctatc gcccgctgcc agcgtttcat
      421 gaccttcggc acggacgtac cgagatacgc ctccagaaac ctgttaaaat atttttccac
      481 ccagaacgcc ggcattgaat atttgagttg cgccaggtct ttgactctcc agcggaactt
      541 gcgacgtacg ggcctgaata cgcgagccat atctatgaga ttgagttcaa gcaagccgtc
      601 gtgttcatgc ataaaaatgt gcgaagcata taaatcgcga tgcacgaaac cggcggcgtg
      661 aaacgttttt accagccgca gaatctggcg attgaaactg tcgagcatgt catggttctg
      721 ctgatatttt tcaaaaaacg catcgccgct tttttcgagc gattggcctg gcacggctgt
      781 gacgactatg aaattccggc ctacgcccca gaaacccaga tgcgcgccgc agatgacagc
      841 cctcattgtc ggcacatcag cctgcctgac cgcacagatg ttgtcaaatt cgtttttagc
      901 ctcgcaccag tgacgtctga acgccgccag ccgacataac ctgcgccaga acgacggagc
      961 ttcatatcgc ttgatgaaca atatccagcc tctgccgtca tcatcatgga attccacgcg
     1021 agtccttcgc cggcggccca acccgggctt gaccatatcg ctgccatcac tgaagctgaa
     1081 cgccccgtcc agagtatcca ggcggtgctt tcgtatgaaa tcaactatcg caggcgcaag
     1141 cggcgactct ttcataaccg tttctccaca ctttcactgc cgagcaattc cctgcatgcg
     1201 gcaagtacca tatccacact tacgccggtg acgcaccgat gatgcgacgg gccggccggc
     1261 agaggacaga attttttctg acaaggcccg cacggcacat ccacgcgaac tatacgttca
     1321 cgcggataat ctatctgtgc ccagaccggg tccgtactgc cgaatatcgt caccacgcca
     1381 cagccgaacg ccgccgcgat atgccgcgtg ccggtatcgt tcgtcaccat aagtgcacaa
     1441 cgtttgacga ggctcttgag cgacgctatc gaattgttcc gtgacgcgaa atccacaagc
     1501 ggccgtgttt tcatgctcgc cgaaactttc gccgcaatag cctgctcatt gggattaggg
     1561 gcggcattga tgattatctg ggcagaaaat tcgtcggcaa gtgcgtcggc aagagcggcg
     1621 aaccgctcag ccggccaaag tttgcttgtg ccgaatgacg caccaggatt gagcatgacc
     1681 aatggcctgg cggcatcgta tccgcactcc gcaagcagac gacaggcttg ctcttcacca
     1741 tctggcgtaa ccggcagttc catcgtgcga ttaacgcaat tcatgcccag cggctcaaga
     1801 agacgggaat aatagtccag catcggcatg ggcttgaaac cggttggact gtcggcactg
     1861 aacacgggca tgattttttc atcaagcaga agagttctgc cgctgcgggc ataaccgatg
     1921 attctcccgc atcgcccaag ccgcgctatc agcgcggaac gaaaactgtt cggcaacagc
     1981 agcgtaacgt cgggagcaaa ctgccgcact gcccgaaccg tggataaaaa attcctcagc
     2041 cgcggttttt tgcgagacgt atcttctata attacgtcag cccaactgct gccggccatc
     2101 accgccagag aaacggctct gccgaaaaac gccactttcg catccggaaa atttttttgg
     2161 cgaatctcgc gcagcacagg cgtagccatc accacgtcgc caacccagtt gggcagaaaa
     2221 accatgattc tgtttatggt tcggccggtc atttgccctc gtccgatgaa agcatcccgt
     2281 ccaagccgcg agcttcaagg tccttccagt cggtgcgaat ctcgctcctc cggaattcaa
     2341 gcggcggagt ttttccaacg aatatcggca tcaaaccggc ttcgcggaaa gccttgccgg
     2401 ccaggctgga ctggcagata ccgacgcgga aattcggaaa aaccgttttg agcgaatcaa
     2461 gctggctgtc cagccgcgac tctatcacta tgcggggcag attgaatcgg ccttcccgca
     2521 ctatatgcca cctctgcttc tgccacaaca atatagggcc gtcgggcaga cctgcacgct
     2581 gaaaccattc gtgattgtta ttctgggtcg tcgaatctct gcgggtaaga taaattatgt
     2641 tggcacggcg ggatatggaa cggagagcag cggcggcttc
//
LOCUS       DAONFI010000321         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_48994_length_2674_cov_4.567392, whole genome shotgun sequence.
ACCESSION   DAONFI010000321 DAONFI010000000
VERSION     DAONFI010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48994_length_2674_cov_4.567392"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1423
                     /locus_tag="PLK08_09260"
     CDS             272..1423
                     /locus_tag="PLK08_09260"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HPS53728.1"
                     /translation="MSFTGKATYTAGSTLPEMAEDVSDIVSIVSPYETPFLDHIGDPQ
                     RAAGSTYHEWLEDTLLPNKDVIADSSIDSSSYDTTITVAHGSRFRVGDQLRAEDSGEL
                     MLVTGVNGNDLTLIRGYGGTTSENLSNAQVIHILGNAALEGDDAPSVRFTNRIRTGNW
                     TQIFTAAVRVSGSDLAVRKLAVADELDYQKQERLRELLRDLENTVINGAAPASDKQGS
                     ASVRRTMHGIIPSITTNVFVPGEDSFPSGALSETQLNLALRMVWENSASRVDTILVNG
                     FQKRKINSFITSTRGYTSKEDTYRDMVSIYESDFGICRVILSRHVPADSVLLLDSSRI
                     DVLPLAGRSFYYKPLASAGDYEAGEVIGEYTLEFRNEMAHGVISGLTTA"
     gene            1626..2273
                     /locus_tag="PLK08_09265"
     CDS             1626..2273
                     /locus_tag="PLK08_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53729.1"
                     /translation="MFCQDRDILCIEPVVYQGQATPPISQPASGTDGEIITSTFTSLS
                     ADFISSGVQAGMVLCTTGTIISEGNVWEIIEVVSSNQLTISSLRASSDGPVISAGNMN
                     GAKYYVRTLAAMIKQVSLELSERFRRLGEAEGISADDFVDSEILAAATAYGVLAKVYR
                     SMADSSISDVRWLKAEHYAEEYERMCNRLCLSVDVNGDGVAEQTRSLGNVRLKRD"
     gene            2285..>2674
                     /locus_tag="PLK08_09270"
     CDS             2285..>2674
                     /locus_tag="PLK08_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53730.1"
                     /translation="MASLENDILQNIIDVLRSCGEFGTVSLGEPRATDKGCLVTILNH
                     SSERVDTGKSRLNPDTWMRLKIQVSISTRSTSSGEGLSRVTALGGIAADVLLADESRG
                     GICSSGIVGRGTEIVKLELAENKGELRE"
BASE COUNT          743 a          649 c          670 g          612 t
ORIGIN      
        1 caggcggatt aatgccgtcg cctactcgtg cctccagagt atcgcaggca tcggaagcat
       61 cgcaactagc cggcctggct cagcgggctg cggtctccgg cagcagaaaa gatatcgctg
      121 aatatctgcg gctgagacga catatttcga caatagcatg agcatgctat tcaaacagcc
      181 aattcatttc taagacatat agacgaaacc ttatagcaag aactggttgt attctctttt
      241 aaaaaatttt caaaatttga aaggaaaaat catgagcttt acaggaaaag caacctacac
      301 cgccggcagc accctgccgg aaatggctga agacgtatcg gacatcgtca gcatcgtcag
      361 cccatatgaa actccatttc tcgaccacat tggcgatcct cagagggcag ccggcagcac
      421 ttaccacgaa tggctggaag acacccttct gccaaacaaa gacgttatcg ctgatagttc
      481 tatcgactca agcagctatg acacgacgat tactgttgca catggctccc gttttcgtgt
      541 tggcgatcag cttcgcgccg aagacagcgg cgaacttatg ctggtaaccg gcgtcaacgg
      601 taacgacctc acgctgattc gcggttacgg cggaacaact tcagaaaatc tttctaacgc
      661 acaggtaatt catatcctcg gcaatgccgc actcgaaggc gacgatgctc cgagtgtacg
      721 attcaccaat cgcatccgca ctggtaactg gacgcagatt ttcacggcag cagttcgtgt
      781 cagcggcagc gacctggctg ttcgcaaact tgcggtagca gacgaactcg attatcagaa
      841 acaggaacga ttgcgtgaac ttctgcgcga tctggaaaac accgtaataa acggtgccgc
      901 tccggcaagt gataaacaag gctcagccag tgtccgcaga accatgcacg gcataattcc
      961 gtcaattaca acaaacgtgt tcgttccagg tgaagacagt tttccgtcag gcgctctgag
     1021 cgaaacccaa ctcaatctcg cgctgagaat ggtatgggaa aattccgcca gccgtgttga
     1081 taccattctg gtcaacggct tccagaaacg taagattaat tcgttcataa cttcgacccg
     1141 cgggtatacc agcaaagagg atacctatcg cgacatggtc agcatttacg agagcgattt
     1201 tggcatatgt cgcgtaattc tctcgcgtca cgtgccggca gactccgtat tactgctcga
     1261 ttcaagccgc atcgatgtcc tgccgctcgc cggccgcagc ttctactata aaccgcttgc
     1321 ctccgcaggc gactatgaag caggtgaagt cattggtgaa tatacgcttg aattccgtaa
     1381 tgaaatggcg cacggcgtta tttcagggct aaccactgcg taatgtcaac gccagggtgc
     1441 gggtgcagtc gttttgcggc tgcacccgcc agcgaaacaa atttcaaaat gaccgattcg
     1501 ccaatatgtc aaaacttttt taaaagcggt tcagcttttt cgactttaca tatcaaaaac
     1561 gaatgcaggt ctgtctgaaa tataaaatcc atacgaaact cacaaactaa tgacaggtga
     1621 agaatatgtt ttgccaggat agagacattc tttgcataga gccggtcgtt taccagggac
     1681 aggcaacgcc gcccatatca cagccggcat caggtacgga cggtgaaata ataacatcga
     1741 catttacaag tctttctgcc gattttattt cgtcgggcgt gcaggcaggc atggtacttt
     1801 gtacgactgg cacaataatt tccgaaggaa atgtctggga gattattgaa gtcgtatcat
     1861 ctaatcaact gactatatca tcgttgcgag cttcgtcgga cgggccggtg atatcggcag
     1921 gaaatatgaa cggggccaaa tattacgttc gtactcttgc cgcgatgata aaacaggtca
     1981 gcctcgaact ttcagaaagg ttcaggcgat tgggtgaagc agagggcata tcggcggatg
     2041 atttcgtcga ttcagaaatt ctggcggctg caacagctta cggagtgctt gcaaaagtat
     2101 atcggagcat ggcggattca tccatcagcg atgtgagatg gctaaaggca gaacattacg
     2161 ccgaagaata cgaacgcatg tgcaatcgct tatgtctgtc agtcgatgta aatggggacg
     2221 gtgtggcaga acagacccgc tcattaggca atgtacgact caaacgcgac tagaggaaca
     2281 aagaatggcc tcgttggaaa atgacattct gcaaaatatt atcgatgttt tgcgcagctg
     2341 cggcgaattc ggaacggttt cactcggtga accgcgtgca acagataaag gctgtctcgt
     2401 cacaatactt aatcactcaa gcgaacgtgt tgacaccggt aagtcccgac tgaatccaga
     2461 tacctggatg cgattgaaaa tacaggtatc tattagcaca cgcagcactt cgtctggcga
     2521 gggtctgtca agagtaaccg cgttaggtgg gattgccgca gatgttctgc tcgcagatga
     2581 aagccgcggc ggcatatgca gcagtgggat tgtcggccgg ggaacagaaa tagtaaaact
     2641 ggaactcgct gaaaataaag gcgaactaag ggaa
//
LOCUS       DAONFI010000322         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_49718_length_2648_cov_4.026996, whole genome shotgun sequence.
ACCESSION   DAONFI010000322 DAONFI010000000
VERSION     DAONFI010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49718_length_2648_cov_4.026996"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..906
                     /gene="lpxA"
                     /locus_tag="PLK08_09275"
     CDS             82..906
                     /gene="lpxA"
                     /locus_tag="PLK08_09275"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPS53731.1"
                     /translation="MSTIHTTAIIDPTARIGENVEIGPYVVIERDTVIGDGCVIRAHS
                     VIGRHTRMGKNNFVDASVSLGGLPQDLKFPHDCVTYVTIGDNNTFREFVTVNRATKEG
                     TSTIVGNNTYWMTTSHAGHDCIIHDNVIMTAGTKAAGHCEIFDGVIMPANGALHQFVW
                     VGKKVMFQGGSQASMHVPPYVLCAQDNNVINLNLVGLKRDASLSMEDIAQIHEAFNLT
                     YRSHLTPTAALEAMDKCTDWGSAAGEFREFVRKVLNAKPPRAKGLCPKISRLAGRR"
     gene            995..1558
                     /locus_tag="PLK08_09280"
     CDS             995..1558
                     /locus_tag="PLK08_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53732.1"
                     /translation="MKKFPVVLFVFTASTLMLAGCTKSKPNVDTAKIDLLAKKLLTSI
                     SQNTPKIPEFYKNNFADSFKERISEKDWKIMAERYRKHLGAMIELDRYSTRIEATEYT
                     IEASCTYCVKWEKEDGKLELSLTQENGQDWKIVSMSITSPKIANLSKMSENENMDAYK
                     KEAESMAAKKASNINTTAPSIPTGEEK"
     gene            1662..2315
                     /locus_tag="PLK08_09285"
     CDS             1662..2315
                     /locus_tag="PLK08_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53733.1"
                     /translation="MNQFKHISPPPPPPIQQSIDGQPLSPANYEQLEYASRQSKKIRN
                     AAGVAAFNGYTFAIFAAISAMAAAFSLTTGEFDFIGIIITLGLGAIAHNELKGRRMLL
                     NFDRRAPKRLGWNQLALLMLIIFYCTWNIAASRTGQSDFDRELGKNSQFNDLYGSEIR
                     SMEKNFTIAIYGTVIIASIIFQGINAIYYFTRKRIMDEYLSKTPPWAIEVIRRTIRT"
BASE COUNT          817 a          646 c          583 g          602 t
ORIGIN      
        1 gcatattcga cacccacccg ccagtatcgg ccaggataaa acgcctgcgc cagttaatgt
       61 gaaatattaa ggattccagt aatgagcacc attcacacca cagcgataat agaccccacc
      121 gcccggatag gcgagaatgt tgaaataggt ccttatgtcg taattgagcg tgacacggtc
      181 ataggtgacg gctgcgttat aagggcacat tccgtcatag ggcggcatac ccgtatgggc
      241 aagaataact tcgtggacgc gtcggtatcg ctgggcggcc tgccgcagga cctgaaattc
      301 ccgcatgact gcgttacata cgtaactatc ggcgacaata acacgtttcg tgaatttgtc
      361 acggtaaatc gcgcgacaaa ggaaggcacg agcaccatcg tcggcaataa tacctattgg
      421 atgaccacat cgcacgccgg ccatgactgc ataattcacg acaatgtaat aatgaccgcc
      481 ggcacgaaag ccgccggcca ctgtgagata ttcgacggcg tcataatgcc agccaacggc
      541 gcactgcatc agttcgtatg ggtcggcaaa aaagtcatgt tccagggcgg ctcgcaggca
      601 agcatgcacg taccgccgta tgtcctctgc gcacaggaca ataatgtcat aaatctcaac
      661 ctggtgggcc tgaaacgcga tgcatcgcta agcatggaag atattgcaca gatacacgaa
      721 gccttcaatc tgacctatag aagccatctg acgccgacgg ctgcattgga ggcaatggat
      781 aaatgtaccg actggggttc ggcagccggc gaattccgtg aattcgtcag aaaagtcctt
      841 aacgcaaagc cgcccagagc aaaagggctc tgcccgaaaa tttcacggct ggccggcaga
      901 agataggcaa aatatcagtt gacttgtacg aaaatatatg gaaaaatctt cctgatatct
      961 caacccacgc aatattttga aatggagtga taaaatgaag aaatttccag tcgtcttgtt
     1021 cgtcttcacg gcatcgacac taatgttagc cggctgcaca aagagcaaac cgaacgtcga
     1081 tactgccaaa atagatttgt tggctaaaaa actgctgacg tccatttctc agaatacacc
     1141 aaaaattcca gagttttaca aaaacaactt cgccgacagc ttcaaggagc gcatttccga
     1201 aaaggactgg aaaatcatgg ctgaacgata ccgcaagcat ctcggcgcca tgattgagct
     1261 cgacagatat tccacccgaa tcgaagcgac ggagtatacc atcgaggcat cgtgcacata
     1321 ctgcgtaaaa tgggaaaaag aggacggcaa acttgagctg tcgctgaccc aggaaaacgg
     1381 gcaggactgg aaaatagtct caatgagcat aacgtccccg aaaatcgcca acctctccaa
     1441 aatgtcggaa aatgaaaata tggacgccta caaaaaagag gctgaaagca tggccgccaa
     1501 aaaggcgtcc aacataaata ctacggcgcc gtccatcccg accggcgaag aaaaatagcg
     1561 tcacattacg acaactccaa aagacctctg tacttccgca gaggtctttt tgtttccaca
     1621 gtcattgact tgcataaacg acagactatg attatcaggt aatgaaccag ttcaagcata
     1681 tttcgccgcc gccaccgccg ccgattcaac aaagcatcga cggccagccg ctatctccgg
     1741 caaattacga acagctggaa tacgcatcac ggcagtcgaa aaaaatccgt aatgccgccg
     1801 gcgtagcggc gttcaacggc tatactttcg caatattcgc agccatttcc gctatggccg
     1861 cagcattcag tctgacaacc ggcgaatttg attttatcgg aataataata actctcggcc
     1921 ttggcgcaat agctcataac gaactcaaag gcagacgcat gctgctgaac ttcgaccgtc
     1981 gtgcgccgaa acggctagga tggaatcagc ttgccctgtt gatgctgata atattttatt
     2041 gcacatggaa tatcgcagca tccagaaccg gccaaagcga tttcgaccgt gaactgggca
     2101 aaaactcgca atttaacgac ctttacggtt ctgaaatacg aagcatggaa aaaaatttca
     2161 ccatcgcaat ttacggaacg gtaataatcg caagtataat attccagggc ataaacgcaa
     2221 tttactattt cactcgaaaa cgaattatgg acgaatatct ttcaaaaacg cctccatggg
     2281 caatcgaggt tatccgccgc acaattagaa cctgatatca ccataccgtt atattattgc
     2341 atatcgaggc ctaaagcata taattctatc tttcacgaca attattgtaa tattttcaaa
     2401 ctgcccgatt tgacagattt tcgcgtacga tgcgaaaata ttttgcaata atgcaataaa
     2461 atatattacc gcggttttca gcagcaacat cgcataaacc gtttgccata tatttcacaa
     2521 ccgcttgcaa atttttaaat tttaagtttt ttaaagtttt tttaaaaaaa ttatcgaacc
     2581 ttatggtgta acgtaagtaa gaggtggcag gatgaaaagg ttttatagcg taatttcagc
     2641 cgtttgtt
//
LOCUS       DAONFI010000323         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_49960_length_2639_cov_4.581656, whole genome shotgun sequence.
ACCESSION   DAONFI010000323 DAONFI010000000
VERSION     DAONFI010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49960_length_2639_cov_4.581656"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1004)
                     /gene="thyA"
                     /locus_tag="PLK08_09290"
     CDS             complement(162..1004)
                     /gene="thyA"
                     /locus_tag="PLK08_09290"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPS53734.1"
                     /translation="MAYIMDAYDDALRYILKHGVKRPDRTGTGTLSVFGYQTRFRINE
                     NFPIITRRKVWPRSIFCELLWLLSGSSDNKDLQEMGVNFWTPWVSEEFEKKHGFNSGE
                     LGPVYGFQMRHFDGDYRTKSGGADQLAYLINEIRTNPTSRRMLVSYWNPNQLDAMRLP
                     PCHYTFQVYIDDEGRMSGMLTQRSADYPVGVPANIQFYSAFIYMLAQQTGYTPWEFVH
                     NTGDTHIYLDQIPAVEAYLSQPAPDSPKLVLNKAADIYSYKPEDFELIDYNPGPVIKI
                     PISV"
     gene            1116..2015
                     /locus_tag="PLK08_09295"
     CDS             1116..2015
                     /locus_tag="PLK08_09295"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HPS53735.1"
                     /translation="MKAAYYLKLARPHQWVKNTLVFAAPVFANHLHIRYAAEWLKSFE
                     AFCAFCCIASATYIVNDLIDCKSDILHPVKRHRPIASGKVGAAEAIIIALIFAIAGFL
                     FAGGCGPLEMTLAAYVVLQIFYNAIFKNRMLADVICIAIGFVLRAIAGAVIISVTISP
                     WLIICTFCLCLFLGFCKRYCEINTLGTDAAAPHRRTLLRYTPALLMHLITLSATLAIV
                     TFLLYAMSDQTLAKFHTQAFIYTLPVIIYCICRAAMLSMNGGFAGPTELLLRDRGLII
                     GVLVWIAMIVLIILFPSKISFLN"
     gene            2027..2398
                     /locus_tag="PLK08_09300"
     CDS             2027..2398
                     /locus_tag="PLK08_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53736.1"
                     /translation="MRYWIFLSIALVLNALANILMKVGAVSEKPVASSSSFLAKTAGF
                     LNVATVAGIILFAANVLVYRKALSGLPISVAYPVMVSVGLLIVVLLARFIPMLDERVS
                     WLQIAGMIFIIAGVWMVSQTR"
     gene            2395..>2639
                     /locus_tag="PLK08_09305"
     CDS             2395..>2639
                     /locus_tag="PLK08_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53737.1"
                     /translation="MKNMADRTPDAMIDNSLSSPEKSAYVFLFRDSGRVLIVSLLILF
                     IWTVALAGMRWYTGSLPFADEGARAGVHLLEQGHRVFS"
BASE COUNT          582 a          647 c          697 g          713 t
ORIGIN      
        1 cagcctcgtc ctcaacgacg caacaaggcg caatttcaaa tttaacatgc tctaggcact
       61 gtctcaaagc ctttaccggc ccgcaaaatt gctcgatttc ggcgctgccc atgttttggg
      121 gataggttct ataaaatatt taaaaaatat tttaaaaaaa ttcagacaga tatcgggatt
      181 ttaattacgg ggccgggatt gtaatcaatc aattcaaaat cttcaggttt gtaactgtag
      241 atgtcggcgg ctttatttag gacgagtttt ggtgaatccg gcgcaggctg tgagaggtat
      301 gcctcgacgg ctggtatctg gtccaggtat atatgcgtgt cgccggtgtt gtggacgaat
      361 tcccacggcg tatagccggt ctgctgtgcg agcatgtaaa tgaacgcact gtaaaactgt
      421 atgttagccg gcacgccgac gggataatcg gctgaccgct gcgtaagcat gccgctcatt
      481 ctgccttcgt cgtcgatgta gacctgaaac gtataatggc acggcggcag acgcatagcg
      541 tcgagctgat tgggattcca gtaactcaca agcatccggc gggacgtcgg atttgtcctg
      601 atttcgttaa taagatacgc caactggtcg gcgccgccgg acttcgtcct gtaatcaccg
      661 tcaaaatgtc gcatctggaa tccgtagacc ggcccaagtt cgccggagtt gaagccgtgt
      721 tttttctcaa attcctcgct gacccacggc gtccagaaat tgacgcccat ttcctgaagg
      781 tctttattgt cgctgctgcc ggagagcagc catagcagtt cacagaagat actgcgaggc
      841 cagactttgc gacgggtgat tatcgggaaa ttttcattta ttcggaatcg cgtctgatag
      901 ccgaacacgc tcagtgtgcc cgtgccggtg cggtcggggc gtttgacgcc gtgtttgaga
      961 atgtaacgca gcgcgtcgtc gtaggcatcc atgatatacg ccatcggtcg tcctttctcc
     1021 tgagcgattg gttgatgagc atcaggatac atcgctgcgg cggcacagtc aataccgctt
     1081 aaggaatcgt cacgattatg ccgatataat accggatgaa agcagcatat tacctcaaac
     1141 tcgccaggcc gcatcagtgg gtgaagaata cccttgtttt cgctgcgccg gtgttcgcaa
     1201 accatctgca tataagatat gccgccgaat ggctcaaatc cttcgaagcg ttttgcgcct
     1261 tctgctgcat agcaagcgct acatatatcg taaacgacct gatagactgt aaaagtgaca
     1321 ttcttcatcc cgtcaagcgg cataggccga tagccagcgg gaaagtcggc gctgccgaag
     1381 cgataataat cgcgttgatt tttgcgattg caggtttttt gttcgcaggc ggctgtgggc
     1441 cactggagat gaccctggca gcgtatgtgg ttttgcagat tttttacaac gccatattca
     1501 aaaatcgcat gttagccgat gtgatttgta ttgcaatcgg gtttgttttg cgggctatcg
     1561 ccggagcggt aatcatcagc gtgaccataa gtccgtggct gataatctgc acgttctgcc
     1621 tgtgcctgtt cctgggcttc tgcaagcggt attgcgaaat aaacacgctt ggtacggatg
     1681 ccgcggcgcc gcatcgcagg acgcttttgc gatatacgcc tgcgcttctg atgcatctga
     1741 taactctttc cgcgaccctg gcaatcgtga cgtttctgct gtatgccatg agtgaccaga
     1801 cgctggccaa atttcacacg caggcgttca tatatactct gccggtaatt atttactgca
     1861 tatgccgcgc ggccatgctg tctatgaacg gcggtttcgc aggccctacg gaactgctgc
     1921 tcagagacag gggccttata atcggcgtat tggtatggat agccatgata gttctcataa
     1981 tcctctttcc gtcaaaaata tcatttctga attgaggaca attatcatgc gatactggat
     2041 ttttctgtca atcgcactgg ttctgaacgc tttggcaaac atattgatga aagtcggagc
     2101 cgtttcggaa aaacctgttg cgtcaagctc ttctttcctg gcgaagacgg ccggttttct
     2161 caatgttgca accgtagcgg gcataatcct ttttgcagca aacgtgctgg tctatcgcaa
     2221 agccctctcc ggcctgccga tttcagtggc gtatccggtc atggtcagcg tcggcctgct
     2281 gattgtggtt ctgctggcca gattcattcc gatgctcgac gagcgggttt cgtggttgca
     2341 aatcgccgga atgatattta tcattgccgg agtatggatg gtgtcacaaa caagatgaaa
     2401 aacatggctg accggacgcc ggacgcgatg attgacaatt cgctttcgtc gccggaaaaa
     2461 tcggcatatg tttttctttt ccgcgactcc ggccgtgtgc tgattgtatc gctgttaatt
     2521 cttttcatat ggactgttgc gctcgccggc atgcggtggt acaccggctc gctgccgttt
     2581 gcggacgagg gtgcaagagc cggcgttcat ctccttgagc agggccatcg ggttttttc
//
LOCUS       DAONFI010000324         2599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_51060_length_2599_cov_3.027516, whole genome shotgun sequence.
ACCESSION   DAONFI010000324 DAONFI010000000
VERSION     DAONFI010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51060_length_2599_cov_3.027516"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="PLK08_09310"
     CDS             complement(<1..1527)
                     /locus_tag="PLK08_09310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPS53738.1"
                     /translation="MTLSAADILAPGGLIEKNLPNYEYRHEQLQMSQAVESAFLQKNH
                     LIVEAGTGVGKSFAYLAPAIIAAAENKRRIVISTFTISLQEQLIRKDLPFLHDILPVE
                     FSAVLGKGRRNYLCFRRLAMTMKGRDKIFASDRQQDQLDLIADWAMRTSTGEYQELDF
                     PVDAAVWNRVCSEPGSCRGGKCPSYHNCHLQSARRKMLAADIVVVNHAMFFADLALRQ
                     DGSQLIGPYDIAVIDEAHTAEDVAGEHFGCHVSSANIGYLLKELYNDETDRGILALAG
                     DAEAISTVKTAAAATDDYFERLAEYHGDGITTSGRFRKEEIIPDTLTQPLKRVADALA
                     RLARSTKDDDQQAELSSLALKAMEFSAGAHNIIFQENEGYAYWKTSHYTSRGNAVHTL
                     ASAPIDVSDILSRVLFSEVQSVILTSATLATARGNSHGFEYIRKRLGVYECSELLLAS
                     PFDYRTQAKLYIETRLGDPNDLESFVPEAARAIGHYALKSAGRCFVLATSYSLLNALA
                     D"
     gene            complement(1524..1997)
                     /locus_tag="PLK08_09315"
     CDS             complement(1524..1997)
                     /locus_tag="PLK08_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53739.1"
                     /translation="MLDMRTKICIWIIVLGMLNFLAYTIGYTIVGGEAIRGRIYVNPE
                     TSQITYFLDSGQQVSHGAFLYSGIHSISIWVTVGAIMLAMLTLAKDRISDSLREAMLR
                     GRTLCSVLAVLIGICTAGLTFQFIHEFIHHFQNPQQLTSQSQPASQPKIIFREDK"
     gene            complement(2032..>2599)
                     /locus_tag="PLK08_09320"
     CDS             complement(2032..>2599)
                     /locus_tag="PLK08_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPS53740.1"
                     /translation="LRNARLGRELLEGIGNPGASWVKLEVLGDKKTLLPDPIGTLEAL
                     EVLKKEGFTVLCYTSDDPIIAMRLKNAGADCVMPAGAPIGSGQGILNQYSIRIILEQL
                     KDGDATYPIIVDAGVGTASDVVFAMELGADGVLLNTGIAGAKDPVAMAVAMNHAMQAG
                     ILARKAGRIPRKLYATASSPIEGVIGTR"
BASE COUNT          533 a          671 c          750 g          645 t
ORIGIN      
        1 atcggcaagg gcgttcagca gcgaatagct ggtggctaat acgaaacagc ggccggcgga
       61 tttcaatgca taatggccta tcgcacgcgc ggcttccggc acgaacgatt ccaggtcgtt
      121 gggatcgccg agtctggttt cgatatagag ttttgcctgc gtccggtaat cgaacgggct
      181 ggccagaagc agttcggaac attcataaac acccagccgc ttccttatat attcaaagcc
      241 gtgagaatta cccctcgccg tagccagcgt agcactcgtg agaattacgc tttgaacttc
      301 ggaaaaaaga acacgactga gtatgtcgga aacgtctatc ggcgccgacg ccaacgtatg
      361 caccgcattg ccgcgggacg tgtagtgcga cgtcttccag taagcgtaac cctcattttc
      421 ctgaaatatg atgttgtgtg ctccggcgga gaattccatt gcctttaaag caaggctgga
      481 aagttcagcc tgctggtcgt cgtcttttgt cgaacgggcc aatcgtgcaa gtgcgtcggc
      541 gacgcgtttg agcggctggg tcagcgtgtc gggtatgatt tcttccttcc tgaatctgcc
      601 gcttgtggta atgccgtcgc cgtgatattc cgccagccgt tcaaaatagt cgtcggttgc
      661 ggcggctgcg gtttttactg tcgatattgc ttcagcatcg ccggccaggg caagtatgcc
      721 cctgtcggtt tcgtcattat aaagttcttt aagaagataa ccgatattgg ctgacgagac
      781 atggcagccg aaatgttcgc cggctacatc ctcggcggta tgtgcttcgt caatgacggc
      841 tatatcgtat gggccgataa gctgtgagcc gtcctgcctg agagcgaggt cggcaaaaaa
      901 catggcgtga tttacgacta ctatgtcagc cgccagcatt ttcctgcgag ccgactggag
      961 atgacagttg tggtaactgg gacatttgcc gccgcggcag cttccaggct cgctgcagac
     1021 gcgattccag acggcggcat cgacggggaa gtcgagttcc tggtattcgc cggtggatgt
     1081 gcgcatggcc cagtcggcaa tcaggtcgag ttggtcctgc tggcggtcgg atgcgaaaat
     1141 tttatcgcgg cctttcatgg tcatggccag ccgcctgaag cagaggtaat tccgccgccc
     1201 cttgccgaga acagccgaaa attccacggg cagaatgtcg tgcagaaaag gcaaatcttt
     1261 ccggatgagc tgttcctgca gcgagatggt gaatgtgctt atgacgatgc gacgtttgtt
     1321 ctctgcggcg gcgattatgg ccggggcgag gtaggcgaaa cttttgccca cgccggtgcc
     1381 ggcttcgact atgagatggt ttttttgtaa aaaagcactc tcgacggctt gtgacatctg
     1441 aagctgttca tggcgatatt catagttcgg caggtttttc tctatcaggc cgccaggcgc
     1501 gaggatgtct gccgccgaca atgtcattta tcttccctga agatgatttt gggctggctg
     1561 gccggctgtg attgcgaagt caattgctgc ggattctgaa aatgatgtat gaactcatgg
     1621 atgaactgga acgtcaggcc ggctgtgcag atgccgataa gcaccgccaa tacgctgcaa
     1681 agcgtgcggc cacgcagcat ggcttcccga agcgagtcgg aaatccggtc tttggccagc
     1741 gtcagcatgg caagcatgat tgcaccgacc gtgacccata tgcttataga atgtatgccg
     1801 ctgtacagga atgcgccgtg gctgacctgt tggccgctgt ccagaaaata ggttatctgg
     1861 ctggtttcag gattgacgta tatcctgccg cggatggctt cgccgcctac gattgtgtaa
     1921 ccgattgtgt aagccagaaa attcaacatg cccagcacga ttatccatat gcatattttc
     1981 gttctcatgt ccaacataat caggtgttct cccgtgtatt tttagggcag tctatcttgt
     2041 gccgatgacg ccttctattg ggctggaagc ggtggcatag agcttgcggg gtatacggcc
     2101 ggctttgcgg gcgagtatgc cagcctgcat agcgtgattc atcgctacgg ccatggcgac
     2161 ggggtccttg gcgccagcta tgccggtatt caggagtacg ccgtccgcgc cgagttccat
     2221 cgcaaatacg acgtcacttg ctgtaccgac gccggcgtcg acgattatcg gatacgtcgc
     2281 gtcgccgtct ttcaactgct caagaattat acgtatgcta tattggttga gtatgccctg
     2341 gccgctgcct atcggcgcgc cggccggcat gacgcagtct gcgccggcgt ttttcagacg
     2401 catggcgatt atcgggtcgt cactggtgta gcagagtacc gtaaagcctt cttttttgag
     2461 aacttccaaa gcttccaatg tgcctatggg gtcgggcaga agcgtctttt tatcgcccag
     2521 cacttccagc ttgacccagc tggcgccggg attgccgatg ccttcgagca gttccctgcc
     2581 cagccttgca ttacggagg
//
LOCUS       DAONFI010000325         2598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_51079_length_2598_cov_3.472277, whole genome shotgun sequence.
ACCESSION   DAONFI010000325 DAONFI010000000
VERSION     DAONFI010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51079_length_2598_cov_3.472277"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLK08_09325"
     CDS             <1..481
                     /locus_tag="PLK08_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPS53741.1"
                     /translation="AACMDYDPNYKVNPPLRSANDVEALRRGIADGTVDCLATDHAPH
                     SKEEKEHEFAVAPFGIISLDCALGLFVKALIEPGIIDWPAMIKCMTVGPAKVIRKPLG
                     TLTPGAAADVTIIDPNMKWTVDVDKFVSKSRNCPYDGWQLGARAVATIVGGKVKFRL"
     gene            complement(482..988)
                     /locus_tag="PLK08_09330"
     CDS             complement(482..988)
                     /locus_tag="PLK08_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53742.1"
                     /translation="MAKKILIAFYSRSGVTLKACSTLAKVLESLGVSATCEEIVDHKS
                     RAGMGGYVIAGKDAALKKTTTIDEPQNIPAEYDMLVLAMPVWAFTVPPAIRAYVDKTK
                     DRLPAKISALCTEGSSGDKRAFCELEKLIDIKLAATVTLIDKKVKTDSAEEYIEPLNV
                     FAKKLLSL"
     gene            complement(1047..2024)
                     /locus_tag="PLK08_09335"
     CDS             complement(1047..2024)
                     /locus_tag="PLK08_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPS53743.1"
                     /translation="MKYKPFHIGSIEIDFPVILAPLAGYSDAPFRSICRQLGAPYATS
                     EMVLDRCVTAKTRKPRPILEKWPNESPVAAQIVGDDPEEMVAAAKAMLQRGYDVIDMN
                     FACPVHKALSRRRGGYMMSQPQRVLEIIRAVRDAIPSPVTLKVRRKFRDEDSEENFWQ
                     IARGAREAGLDAITVHARSVEQKYTGVADWDFLRRVKQQFQDWTVIGSGDVLTAAAAL
                     KMLDETGVDAAAVARGVLGNPWIFRQARELAAGKPEKWPSIAEQKAIMLRHMDDVVRL
                     YGLKGPGMMRKFGIKYARLHPQAKKVRMAFVNIKTQEQWNAVLDEYYNE"
     gene            complement(2021..2317)
                     /locus_tag="PLK08_09340"
     CDS             complement(2021..2317)
                     /locus_tag="PLK08_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53744.1"
                     /translation="MTDVHPSPSAVTTATSSQSAVQTPGEHPQPIRNIERLSTDESQA
                     IRKDPAILEMLDIFKGEIRDIFKDLPAKNATNQPATGTSQPIHSADTDDYDESE"
     gene            complement(2314..>2598)
                     /locus_tag="PLK08_09345"
     CDS             complement(2314..>2598)
                     /locus_tag="PLK08_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53745.1"
                     /translation="GPTSDLIELPETRKKQLAELASRFSLPVIVQSVGILQNVARNIR
                     GSSVSRALVEAAIVRLAEADKFIDPQSIVARLEQLAAGGMAMTVDAKKKI"
BASE COUNT          532 a          684 c          699 g          683 t
ORIGIN      
        1 cgcagcctgc atggattacg acccgaatta caaggttaat ccgccgctgc gttccgcaaa
       61 cgacgtcgag gcgttgcgac gcggcatagc cgacggcact gtagactgcc ttgccaccga
      121 ccacgcgccg cactcaaaag aggaaaaaga acacgaattt gcggttgcgc ctttcgggat
      181 tatttcgctg gactgtgcgt tgggtctgtt cgtcaaggcg cttatcgagc ctggcataat
      241 agattggccg gctatgatta agtgcatgac cgtcggcccg gcgaaagtca tccgcaaacc
      301 gctgggcacg cttacgccag gcgccgcggc agacgttacg ataatcgacc cgaacatgaa
      361 atggacggtt gacgtggata aattcgtttc gaaaagccgc aactgtccgt acgacggctg
      421 gcaacttggc gctagggcgg ttgcgactat agtcggcggc aaggtcaagt tccggctgtg
      481 attacagcga taagagtttt tttgcaaaaa cgttcagcgg ctcgatatat tcttcggctg
      541 agtcggtttt gacctttttg tcgataagcg tgacagtggc tgccagcttg atgtctataa
      601 gtttttccag ctcgcaaaag gctcttttat cgccggaact gccttccgta cagagagcgg
      661 aaatttttgc cggcagccgg tcttttgttt tatcaacata tgccctgatg gctggcggaa
      721 cggtgaacgc ccacaccggc atagccagca ccaacatgtc gtactctgcc ggaatatttt
      781 ggggctcatc gatagttgta gtttttttta acgcggcatc tttgccggcg attacatacc
      841 cacccatgcc ggcacggctt ttatggtcta cgatttcttc acatgttgca gacacgccga
      901 ggctttcgag aactttggca agagtcgaac atgccttgag cgttacgccg cttcgcgaat
      961 aaaacgctat taaaatcttt tttgccatgg cagactcctt ttttcaatgc tctataaaat
     1021 accggtaaaa attacgcttg catgttttat tcgttataat actcgtcgag aacggcgttc
     1081 cactgttcct gcgtttttat atttacgaac gccattcgta ctttttttgc ctgcggatgg
     1141 agtcgtgcgt attttatgcc gaattttcgc atcatgcccg gccccttcag cccgtaaagt
     1201 ctgacgacat cgtccatatg tcgtaacatt atagccttct gttccgcaat cgacggccat
     1261 ttttccggtt tgccggcggc taattcgcgt gcctgccgga aaatccacgg atttcccaat
     1321 acgccgcgtg cgaccgctgc cgcgtccacg ccggtttcgt cgagcatttt caaggctgcc
     1381 gccgcggtaa gtacgtcgcc tgaacctatt accgtccagt cctggaactg ctgctttacc
     1441 cgtcttaaaa aatcccagtc agctacgccg gtatatttct gttccacgct gcgggcgtga
     1501 acggtgatgg catccaggcc ggcctccctg gcgccacggg ctatctgcca gaaattttct
     1561 tcggaatctt catccctgaa ttttcggcga actttgagcg tgacgggcga tggtattgcg
     1621 tcgcggactg ccctgatgat ttccagaacc cgctgcggct gcgacatcat atatccgccg
     1681 cgtcgccgtg aaagggcttt gtggacagga caggcgaaat tcatgtcgat tacgtcgtaa
     1741 ccgcgttgaa gcatggcttt tgcggcggcg accatttctt cggggtcgtc gcctacaatc
     1801 tgagccgcca ccggcgactc gttcggccat ttttccagta tgggccgcgg cttgcgagtt
     1861 ttggcggtta cgcaccggtc gagcaccatt tcactggttg cgtacggcgc gcctaattgg
     1921 cggcatatcg aacggaacgg cgcatccgaa taaccggcta acggcgcaag tatgaccgga
     1981 aaatctatct caatactgcc gatatggaaa ggtttgtact tcattctgat tcatcatagt
     2041 cgtcggtatc ggcggaatgt atcggctgcg atgttccggt ggcaggctgg ttggtcgcat
     2101 tttttgcggg caaatcctta aaaatgtccc taatttcgcc cttgaaaatg tcgagcattt
     2161 ccaatatggc cgggtctttg cgtatcgcct gagactcatc agtgctaagc cgctcgatat
     2221 ttctgatggg ttgtggatgt tcaccgggcg tctgaacggc cgattgagat gatgtggcgg
     2281 ttgtaacggc ggagggcgac ggatgaacgt cagtcaaatt ttttttttag cgtcaactgt
     2341 catcgccatg ccgccggcgg caagctgttc gagcctggcg acaatgcttt gcgggtcgat
     2401 gaacttatca gcctcggcga ggcggactat ggcggcttca accagggcgc ggcttaccga
     2461 tgagccgcgg atatttctgg ctacgttctg cagtatgccg accgattgta cgataaccgg
     2521 cagagaaaat cggctggcca gttctgcgag ctgttttttg cgagtttccg gcagttctat
     2581 aaggtcgctg gtcggccc
//
LOCUS       DAONFI010000326         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_51104_length_2597_cov_4.708497, whole genome shotgun sequence.
ACCESSION   DAONFI010000326 DAONFI010000000
VERSION     DAONFI010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51104_length_2597_cov_4.708497"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..2292)
                     /locus_tag="PLK08_09350"
     CDS             complement(94..2292)
                     /locus_tag="PLK08_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53746.1"
                     /translation="MALRFFRKRRKLILIIMIVLMVSFLVSIQGLSSVLSSKAGSEVV
                     GTSDAGKVTLGRMQEGKMELDIIGRLARSGAFERVPSQIYAYYALQQNKADRQTLAYV
                     LLIESAREQGIDVTAGDVDSAINAYKEANFPFDRFMDQMVTFYSFTRSQVKDIIANWL
                     MVVKAYQANNSNYSPSREELQHVYVDINSKMQVKAIRIPASDFVSKIPAPSMDKIREQ
                     FQALKNRPAGDFTGVDSFRFGYLIPAKLNVAWLMADYNAFARVEQPTAEQIEDYYNKN
                     REMFAKDPKAEKKQYKSLSEVRWEIFTQLQMQMAQTTYAEAVTLAGAAVRDELAGAQA
                     ASVAKKSDYSQVMKAARAELVKPADEMLSRIIPVIYIEKAPLAEAINQIASLAQPRLK
                     GISFPYGLEGGKIRIDPGISVSLNAQNITVGDALAKLIKDNKLGLDVGWIRFAPMDNV
                     LFCDALAPVNCGDTGMITLEELNADAFLSSCFSREARATLPMLAASVKELGTNSEFTA
                     GILGPVLQSFNPDKAGEIYWMITKVEPAHAPEELTADIAKEIGDDIRTSEALSLAVKV
                     ADEIRTPAQFDEYIKKNKVMPILTEPFSLMILSHSAYGQSGFGQGSVPNMKFCNAAVD
                     KVFIEKAFEILAPKDIAKPYTQASDNLMQLKLPCEDCVVVARRVDFMPALANDFDEQA
                     KEYRKILDGAQMAITLLNWYNLDNIVKRTGFQETQQGYLLQKTGQDDGND"
BASE COUNT          658 a          690 c          548 g          701 t
ORIGIN      
        1 cctcgtcctc aacgacgcaa caaggcgcaa tttcaaattt aacatgctct aaaattaccg
       61 tacagggacc gtctcaataa atctcatcaa caatcaatca ttaccatcat cctgaccagt
      121 cttctgcaac aaataaccct gctgcgtctc ctgaaacccg gtacgcttta caatattatc
      181 cagattatac cagtttaaca gcgttatcgc catctgtgca ccatcgagaa tttttctata
      241 ctctttagcc tgctcgtcaa aatcattcgc caatgccggc ataaaatcga ctcgccgtgc
      301 cactacaaca caatcttcac acggcaattt caactgcatc aaattatcag atgcctgcgt
      361 ataaggcttg gcaatatcct tgggcgccaa aatttcaaat gccttttcaa taaacacctt
      421 gtcaacagca gcattgcaga acttcatatt cggcacactg ccctgaccga aaccactctg
      481 cccatacgcc gaatgcgaaa gtatcataag actgaacggt tctgtcaata tcggcataac
      541 tttatttttc ttgatatact cgtcaaactg tgccggcgtt ctgatttcat cggcaacttt
      601 taccgcaagg ctaagtgcct cagaagttcg gatgtcatca cctatttctt tcgcaatatc
      661 ggcggtaagt tcttcaggag catgagctgg ttcgactttt gtaatcatcc agtaaatttc
      721 gccggcttta tcggggttaa aactttgcag aacagggccg agaatgccag ccgtaaattc
      781 gctattcgta ccaagttctt taacactggc cgccaacatg ggcagggtag cgcgggcttc
      841 acgagaaaaa caactgctca gaaatgcatc ggcattgagt tcttcgagag taatcatgcc
      901 ggtatcccca cagttgacag gcgcaagcgc atcgcagaat aatacattat ccattggtgc
      961 aaaccgaatc cagccgacgt caagacccag tttgttatct tttatgagtt tggccagcgc
     1021 atcgccgacc gttatattct gggcatttag cgaaacgctt atgccagggt ctattctgat
     1081 tttgccgcct tccaggccgt acggaaagct gatacctttc aaccgcggct gtgccaggct
     1141 ggctatctgg tttatcgctt ctgccagcgg ggctttttct atataaatca caggaataat
     1201 ccggctgagc atttcgtcgg caggcttgac aagctcggca cgggcagcct tcataacctg
     1261 gctatagtcg cttttctttg cgacgctcgc agcctgcgca cccgcaagct catcgcggac
     1321 agccgcacca gccagcgtaa cagcctctgc atacgtcgtc tgagccatct gcatctgaag
     1381 ctgcgtgaaa atttcccaac gaacttccga aagcgattta tactgtttct tttcagcctt
     1441 cgggtctttg gcgaacattt cacggttttt attataataa tcttcaatct gttcggcagt
     1501 cggctgttcg acacgggcaa aggcgttgta atcagccatc agccacgcga cgttcaactt
     1561 cgccggaata agatagccaa atctgaaact atccacgccg gtaaaatcgc cggcaggacg
     1621 atttttcaat gcctgaaact gttcccggat tttatccatc gaaggcgccg gtatttttga
     1681 tacaaaatcc gacgcaggaa tacgaatcgc ctttacctgc attttggaat ttatatcgac
     1741 ataaacgtgc tgaagttctt cccgactagg tgaatagttg gaattgttgg cttgatacgc
     1801 ttttaccacc atcagccagt tggcaatgat gtccttcacc tgtgaccgcg tgaaagaata
     1861 aaacgtcacc atctgatcca tgaaccggtc aaacgggaag tttgcctcct tatatgcgtt
     1921 gattgccgag tcgacatcgc cggcagtgac atcgatgccc tgttccctgg ctgattcaat
     1981 cagcaggaca tatgccagcg tctgccggtc agccttgttt tgctgcagcg cgtaatatgc
     2041 ataaatctgc gacggcacac gttcaaaggc accgctgcgg gcaagtcggc cgatgatgtc
     2101 cagttccatc ttgccttcct gcatccggcc gagcgttact ttgcctgcat cacttgtgcc
     2161 gacgacctcg ctgccggctt tgctgctaag aaccgacgag agtccctgaa tggataccag
     2221 aaaactgacc atcaggacta tcatgattat caatatcagt tttctgcgtt tgcgaaagaa
     2281 cctcaacgcc atttcattct ccttgccggc tggcagccgg taaactataa tttaattaca
     2341 acgcgaaaca gcaatgatag ttttttttac ttattttgca agtttttgca gtgaatttct
     2401 gcggattgaa tctcttaacg ttttcaagcc gccgaattac gaacagccgt ttagagcagt
     2461 tctccaaaca ccaccagaaa ggacttattt gcctatgctg cctggttcac atttaagcgt
     2521 agccggcgga cttgccaacg cattgaccgc cgcggaagat tacaaatttc aatcggtggc
     2581 actttttgtg cgaaatc
//
LOCUS       DAONFI010000327         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_51525_length_2584_cov_3.952946, whole genome shotgun sequence.
ACCESSION   DAONFI010000327 DAONFI010000000
VERSION     DAONFI010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51525_length_2584_cov_3.952946"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1484)
                     /locus_tag="PLK08_09355"
     CDS             complement(90..1484)
                     /locus_tag="PLK08_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPS53747.1"
                     /translation="MLGVGCVLLFLAIRKGFEPLLLIPIGFGCILSNIPLAGITDCTP
                     PATEYNYFLAQEYSKMDNAKIVAIADNADPNNNLLASIEKEYINHMVARAEGEQAKAA
                     AKKTPTVSEKAGKMVINPDFASYLRTQKSVAVAAFNSFYAAKAAAAGEKFKPYQETDG
                     FLTIIYETGVATGLFPLIIFMGVGAMTDFGPLLANPKTALLGGAAQLGIFTTLFGALM
                     LGFDINQASAIGIIGGADGPTSIFVARKLAPDLLGAIAVAAYSYMALVPIIQPPIMRL
                     LTTKKQRAIKMEQLRHVSKLEKILFPLVVLGLCAVLLPSACPLIGALMFGNLAKESGV
                     VERLSTTMANALINIVTIFLGLSVGMKLAADKFLNLQTIYILVLGLIAFSFGTAGGVI
                     MAHIMNMFSKNKINPLIGAAGVSAVPMAARVCNKVGQEEDPHNFLLMHAMGPNVAGVI
                     GSAVAAGVLLALCK"
     gene            complement(1595..>2584)
                     /locus_tag="PLK08_09360"
     CDS             complement(1595..>2584)
                     /locus_tag="PLK08_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HPS53748.1"
                     /translation="YFMPPEASAVEPLIPWSPMPGGALTANTQMLRDNGIMDKYDDCI
                     KAMSEVVCRGGFGTSVTPVSQFYFQQAFNNVMFGPWKKIAEGYGKMVLGYFGKTPVAP
                     DEEIVKLAADQLKLEPTTQDPRLLNDGNPAKGVAAAKKKLTEAGITDLSDENIFIAAA
                     CGDKGIAFLKGNCTLGVRKNEPASKAATGTAASVKSANANGAYCVTLNGKKYNVQLDG
                     GKAVVNGRSYNYAVGEGFAAAVGSGNGNGNGGVAGLSSAADHVVKAPLPGVIVRMEAA
                     EGDSVSEGDVLMVMEAMKMETEIKSQFSGTIARVLVKQGDQINASDELVHISA"
BASE COUNT          594 a          741 c          639 g          610 t
ORIGIN      
        1 aaaaacccgc atgcgacgac ttccgccgct acacgcgggt caatgttttg ccttacgata
       61 tcttaaaatc cggcgtttac agccgtcctt catttgcaca gtgccagaag aacgccagcc
      121 gccaccgctg acccgataac gccagccaca ttcgggccca tggcatgcat gagcagaaaa
      181 ttgtgcgggt cttcttcctg gccgacctta ttgcagacgc gggcagccat cggcacggcg
      241 gatacgcccg cagctccgat aagcggattt atcttgttct tgctgaacat attcattatg
      301 tgtgccataa tcacgccgcc ggctgtgccg aaactgaacg ctatcaggcc cagaaccaat
      361 atgtatatcg tctgaagatt aaggaatttg tccgctgcca gcttcatgcc gacggaaagg
      421 cccaggaaaa tcgtaactat attgataaga gcgttggcca tcgtcgtgct cagacgctca
      481 accacgccag actccttggc cagattgccg aacatcagtg cgccgataag cggacatgca
      541 ctgggcaaca gcaccgcaca aaggcccaaa actaccaacg ggaaaagaat cttttccaac
      601 ttggaaacat gcctgagctg ttccattttt atggctcgct gctttttggt agtcaacagc
      661 cgcattatgg gcggctgaat tatcggcaca agggccatgt aactgtatgc cgccacggca
      721 attgcgccca gcaaatccgg cgccagcttg cgagccacga atatcgacgt cgggccgtcc
      781 gcaccgccga taatgcctat cgccgacgcc tggttaatat cgaagcccaa catcaacgca
      841 ccaaaaagcg tggtaaaaat gcctaactgt gcagctccgc cgagtagtgc cgtcttggga
      901 ttggcaagca gcgggccgaa gtcggtcata gctcctacgc ccatgaaaat tatcagcgga
      961 aacaggcccg tagctacgcc ggtttcataa ataatcgtta aaaaaccgtc ggtttcctga
     1021 taaggtttga atttttcgcc ggcagcggcg gctttggcgg cataaaagct gttgaacgcc
     1081 gccacagcta ccgacttctg cgttcgtaaa tagctggcaa aatcggggtt tataaccatt
     1141 ttgccggcct tttcgctgac agtgggagtc tttttcgcgg cagctttagc ctgctcgcct
     1201 tcagccctcg ccaccatgtg attgatatat tccttctcaa tgcttgccag cagattgttg
     1261 ttcgggtcgg cattgtcggc gatggcaact atttttgcgt tatccatctt tgaatattcc
     1321 tgggcaagga aataattgta ttccgtcgcc ggcggcgtac agtcggtaat gccggctagc
     1381 ggaatattcg agagtatgca gccaaagcct attggaatca gcaacagcgg ctcaaacccc
     1441 ttgcggatag ccagaaacag cagcacacag cctacgccca gcattaccag attctgccag
     1501 cctgtgcccg tgacgaactg actcaggccg gtctgcaacc acaggtcgtg gaaacattct
     1561 actatattca tgatactgct cccttatata ttgctcaggc gctgatatga acgagttcat
     1621 cgctggcatt tatctggtca ccctgcttga caagcacacg ggcaatagtg ccgctgaact
     1681 ggcttttgat ttccgtttcc atcttcatgg cttccataac catgagcacg tcgccttccg
     1741 aaacgctatc gccttccgca gcttccatgc gaactataac acccggcaga ggcgctttca
     1801 cgacatggtc agccgccgat gaaaggccgg cgacaccacc attgccgttg ccgttaccac
     1861 tgccaacagc tgcggcaaaa ccttcaccaa cggcataatt gtaacttctg ccgttgacaa
     1921 ccgccttgcc gccgtcaagt tgcacattat acttcttgcc attgagcgta acgcagtatg
     1981 cgccgttcgc attcgcactt ttgacggatg cggctgtgcc ggtggcggct ttggaagcag
     2041 gctcgttctt gcgaacgccc agcgtgcaat tgcccttcag gaacgctata cccttatcgc
     2101 cgcaggctgc ggcaataaat atattctcat cgctcaggtc ggttatgcct gcttcggtaa
     2161 gtttcttttt agctgcagcg acgcctttgg ccggattgcc gtcattgagc agacgaggat
     2221 cctgcgtagt cggttcgagc ttgagctggt cggcggctaa ttttacgatt tcttcgtccg
     2281 gcgcaaccgg cgtcttgccg aaataaccca aaaccatttt gccgtaaccc tcggcgattt
     2341 tcttccacgg cccgaacatg acgttgttga acgcctgctg gaaataaaac tgacttaccg
     2401 gcgtaacgct cgtgccgaaa ccgccgcggc aaacgacttc gctcatggcc tttatgcaat
     2461 cgtcgtactt atccataatc ccgttatcgc gcaacatctg cgtattggcg gtaagtgcgc
     2521 cgccgggcat gggcgaccac ggaataagcg gttcgacggc agaggcttcg ggcggcatga
     2581 aata
//
LOCUS       DAONFI010000328         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_51784_length_2576_cov_4.071400, whole genome shotgun sequence.
ACCESSION   DAONFI010000328 DAONFI010000000
VERSION     DAONFI010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51784_length_2576_cov_4.071400"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLK08_09365"
     CDS             <1..532
                     /locus_tag="PLK08_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53749.1"
                     /translation="DTPCPGYVACYKQISAPPTKLVLQYTTLLYPFEGTKRPDASFKD
                     GVVKIGQNREGLYMRKGEKTDGDCAFVGKEDGAINRILLVTGSRISNAVQLDVPADFM
                     IVTRQGESLDIQLMESLSKKVNRIELAGFQGVSKVMVNGKSCKVSETDGKLVIVGPFE
                     KIVPNPDNPDLFWIVK"
     gene            915..1967
                     /locus_tag="PLK08_09370"
     CDS             915..1967
                     /locus_tag="PLK08_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPS53750.1"
                     /translation="MGTLVSQIAKKAGVAVSSAYEILSHPENSRYQEKTCKKVLEASR
                     ELDYRPHAAAKAIRTGRFECITLLMSTAYTHSYLPNNLLYGIHDELDRRNIHLTINRL
                     SDEMLTNEKYLPKILRYQMSDGLLINYTNYLPPQVESVIKQSCLPAVWINFKRDFCCV
                     YPDSFLAGRQATEYLLKMGHRRIAFLDYLFNQAEFVGAHFSINERCKGYETAMQEAGL
                     SPRIIRPESGRIEPGEEIAYTQNWLRQPDRPTAVIAYWVASALPVMQGVLAAGLSVPR
                     DLSIVTFNSETFGGNLLRESGLYINSMIEPEFEMGTQAALMMIKKIKKPSDEIPSCRL
                     EFKLEEFGTCCPPKAK"
     gene            2040..>2576
                     /locus_tag="PLK08_09375"
     CDS             2040..>2576
                     /locus_tag="PLK08_09375"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HPS53751.1"
                     /translation="MEKMKIKLKKISVIAILTCVFATCCGAFGDTVTLAPTDDTYVNQ
                     QWAASNFGTATTVGIGFGLDTPANRQGWIYLKFGLDSIPDGATITSATVYLYKPWVHA
                     NNDFTAGIYNVSDDSWTESTLKWTNKPDTAVTATDNQSITTASVQWYSWDVTTLVASN
                     NINGDDILSVCLKENPQQT"
BASE COUNT          758 a          521 c          607 g          690 t
ORIGIN      
        1 cgatacgccc tgtccgggat atgtcgcatg ttataagcag atttcggcac cgccgacaaa
       61 gctggttctt cagtatacga cgctgctata tccgtttgaa ggtacgaaac ggccggatgc
      121 ttcatttaaa gatggcgtag tcaaaatcgg tcaaaatcgc gaaggcctct atatgcgcaa
      181 aggcgaaaag accgacggcg actgtgcgtt cgtcggcaaa gaagatggag cgattaatcg
      241 catacttctt gttaccggta gccgcatatc caacgcggtt cagctcgacg ttcctgctga
      301 ttttatgatt gtgacgcggc agggcgagtc gctggacata caattaatgg aaagtttgtc
      361 caaaaaagtc aatcgcatag agctggctgg ctttcaaggc gtcagcaaag taatggtgaa
      421 tggcaaatca tgcaaggtat ccgaaactga cggcaaattg gtaattgtcg ggccttttga
      481 aaaaatcgtg cccaatcccg ataatcccga tttattctgg attgtaaaat aatcccaagc
      541 cgtaagccat tgttgctatt atcgttaagt taaaaatagc ctgacttctg gcgtatgtgc
      601 attatattgc cctgtacgtc aggagtcagg ctatctgtat tttgtcttaa aaaatatctc
      661 tgaagtataa ctgtgggaaa ttatcgcatt taacgcatat atcacgtaaa tttcagtttg
      721 aaaagaaatt ttttacttga ttatcgtaac gtttcgatta atatatgtgg taacgttatg
      781 attaattggt aaccaaccag tgaatagtta aagttcaaaa tgatagtatc ccaggcttcg
      841 ccaagctgac atgttataat attatagata gagtaaatgt tatcaattat tgattattta
      901 ggaaacatgg cataatggga acgttagttt cacaaatagc gaaaaaagcc ggcgtagctg
      961 tttcaagcgc atatgaaata ttgtcgcacc ctgaaaactc caggtatcag gagaaaacgt
     1021 gtaaaaaagt actggaagcc tcgcgtgagc ttgattatcg gccacatgcc gcggctaagg
     1081 ctatccgtac cggcagattt gaatgcatta cactcctgat gagtaccgca tatactcaca
     1141 gttaccttcc gaacaacctg ctttacggca tccatgacga gcttgacaga cgtaatattc
     1201 atctgactat aaaccggctg tcagacgaaa tgctgacgaa cgagaaatac ctgccaaaaa
     1261 ttttgcgtta tcagatgtct gacgggctgc ttataaacta caccaactat ctgcctccgc
     1321 aggtggagtc tgtcataaaa cagtcctgtt tgccggcggt atggataaat tttaagcgtg
     1381 atttttgttg cgtttatccc gatagttttc tggctggccg acaggcgacg gaatatctgt
     1441 tgaagatggg ccatcgtcgt attgcttttc tggactatct gttcaatcag gcggaatttg
     1501 tgggggcaca tttcagtata aacgaacgct gcaaaggtta tgaaacagcc atgcaggaag
     1561 ccggcttgtc gccgcgtatc attcggcctg agtccggcag aattgagcca ggcgaagaaa
     1621 tcgcttatac gcagaactgg ctgaggcagc cagacaggcc tacagcggta atagcgtact
     1681 gggttgccag tgcactgcct gtcatgcagg gagtgttggc ggctggccta agtgttccac
     1741 gcgatttatc tatcgtgact tttaacagtg aaacgttcgg cggcaattta ttgcgggagt
     1801 caggtctcta tatcaactca atgattgaac ctgagtttga aatgggaact caagcggcat
     1861 tgatgatgat aaaaaagata aaaaaacctt cagatgaaat tccttcctgc aggctcgaat
     1921 tcaagttgga agaattcgga acatgctgtc cgccaaaggc taaataacat ttttaagcgt
     1981 aaaaacatga agcgtataaa aaattcagca acgaaaacca gtttagaaag gaaatggcca
     2041 tggaaaagat gaaaatcaaa ttgaaaaaga tttcggtaat tgccatattg acatgtgttt
     2101 ttgcgacatg ttgcggtgcg ttcggtgata ctgtgacatt ggctcctact gatgatacgt
     2161 atgtcaatca gcaatgggct gccagcaatt ttggtacagc tacgacagtg ggaataggct
     2221 tcggcctaga tacgccggct aatcgtcaag ggtggattta ccttaaattc ggcctggatt
     2281 caatcccgga tggtgcaacg ataacatcgg ctaccgttta tttgtataaa ccttgggttc
     2341 acgccaacaa tgattttacc gcaggtattt ataatgtctc tgacgatagc tggacggaaa
     2401 gcactcttaa atggactaac aaacctgaca ctgcggttac ggcaactgat aatcagagta
     2461 ttacgactgc aagcgtacaa tggtacagtt gggacgtaac gacactggta gcttcaaaca
     2521 atataaacgg cgatgatatt ctgtccgtat gtttgaaaga aaatccacag cagacg
//
LOCUS       DAONFI010000329         2554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_52411_length_2554_cov_4.762305, whole genome shotgun sequence.
ACCESSION   DAONFI010000329 DAONFI010000000
VERSION     DAONFI010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52411_length_2554_cov_4.762305"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..2433)
                     /gene="fusA"
                     /locus_tag="PLK08_09380"
     CDS             complement(343..2433)
                     /gene="fusA"
                     /locus_tag="PLK08_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPS53752.1"
                     /translation="MKRRGKIRNIGISAHIDSGKTTLTERILFYTGRIHRMQEVHGSG
                     DGATMDFMELEKERGITITSAATSVHWKDNGINIIDTPGHVDFTVEVERSLRVLDGAI
                     MVLCSVGGVQSQSVTVDRQMKRYKVPRIAFINKMDRQGADPMRVRADMVEKLGLNVVP
                     VQINMGAGDDFVGVVDLIRMKALTFEGSQGENVVVSDIPAEYADRAAEARTEMLDALS
                     MFNDQMMELMLEDNHVPEEMIEDTIRQATINRDIVPLMMGSAFKNKGVQPLIDAVCRY
                     LPSPLEAPSFARDNNNSGAEVKLECDPDKPLVAMAFKIVDETFGQLTYTRIYQGKVAK
                     GQTYRNSRTNQNVRIGRIVKMHADDREDIPEALAGDIVAFVGVDCASGDTFCSPEISY
                     SLESIYVADPVISLAVIPESSKDQDQMAKALNRFMREDPTFRVSSDPETGETIIAGMG
                     ELHLDVYVERMKREYKAAVTVGKPNVSYREAPTKETPYDYRHKKQTGGSGQFAHVAGK
                     MIPLPEDSEEMYVFEDLISGGAIPREYIPAVDKGFREALKKGPVAGYEIVGVKMTLED
                     GSFHAVDSSEMAFRICAMDAFKQAFEKSKPCLLEPIMLVEIETPSDFQGAIVGDLNSR
                     RGMILNTENRENYSIIKAEVPLANMFGYATIIRSLSKGMATFTMEMSKYAKVPNNIAE
                     EVIEERRKQHEKKK"
BASE COUNT          531 a          659 c          632 g          732 t
ORIGIN      
        1 cggatacttt catctacggt ttaaaaaccg tagaattacg aaggacgtat taaaaaaatt
       61 caatctgcag gtattgcaag caacaagatt cgacgaccaa tttttaagca atttttaatt
      121 ttcaatttct tctaactttc aagatggaac ggcgtccaat cgcatttctt gaaacttgtc
      181 atttttaaat tgcttgtaat ttgtttcttg ctacttgaac ctatcccaaa acagtatttt
      241 atcaattttc accctatcgg gcaattccca atcccgtcaa taaaaaaacg cccagccatg
      301 tggtcgggcg ttttttgtgg atttgtctca ttgccgaaaa aattatttct tcttttcatg
      361 ctgttttctg cgttcttcga tgacttcctc ggcgatgttg ttgggcactt tcgcatactt
      421 gctcatttcc atcgtgaatg tagccatgcc cttcgatagc gagcggataa tcgtcgcata
      481 accgaacatg ttagccagcg gcacttcagc ctttattatg gagtagtttt cacggttttc
      541 agtattcaga atcatgccgc ggcggctgtt gaggtcgccg acgatagcac cctggaaatc
      601 gctgggcgtt tctatttcaa ccagcatgat aggttcgagc aggcatggtt tggacttctc
      661 gaatgcctgc ttgaaggcgt ccatcgcaca gatacggaaa gccatttcac ttgagtcgac
      721 cgcgtggaaa ctgccgtctt ccagcgtcat cttcacgccg acgatttcat atcctgccac
      781 aggccctttt ttcaaggctt cacgaaagcc cttgtcgacg gctggaatgt attcacgtgg
      841 aatcgcaccg ccgctgataa ggtcttcaaa gacgtacatt tcttccgaat cttccggcag
      901 cggtatcatt ttgccggcga cgtgcgcaaa ctggccgctg ccgccggtct gttttttatg
      961 acgataatcg taaggagttt ctttcgtcgg cgcttcacgg tagctgacgt tcggtttgcc
     1021 gacagtgacg gcggctttgt attcgcgttt catgcgttcg acgtagacgt ccaggtgcag
     1081 ctcacccatg ccggcgatga tggtttcgcc tgtttcaggg tcgctggaga ccctgaaggt
     1141 ggggtcttca cgcatgaagc ggttaagcgc cttggccatc tgatcctggt ctttggaact
     1201 ttcgggtatg actgccaggc ttatcaccgg atctgccaca taaatactct ctagcgaata
     1261 gcttatttcc ggcgagcaga acgtatcgcc gctggcgcaa tccacgccga cgaaggctac
     1321 tatatcgccg gcgagcgctt cggggatatc ttccctatcg tcagcatgca tttttacgat
     1381 tcgtcctatg cggacgttct ggttcgtgcg gctgttgcga taggtctggc ccttagccac
     1441 tttgccctga taaatacggg tgtaggtaag ctggccgaaa gtctcgtcga ctatcttgaa
     1501 cgccatggcc acgagcggct tgtcgggatc gcattccagt ttgacttcag cgccgctgtt
     1561 gttattgtca cgcgcaaaac tgggggcttc gagcgggctg ggcagatagc ggcataccgc
     1621 gtctatgagc ggctgaacgc ccttgttctt gaacgctgaa cccatcatca gcggcacgat
     1681 gtcgcgattg atggtcgcct ggcgaatagt gtcttctatc atctcttcag gaacatgatt
     1741 gtcttcaagc atcagttcca tcatctggtc gttaaacatg ctcagcgcgt ccagcatttc
     1801 cgtgcgagcc tcggcggcac ggtcggcgta ttccgccgga atatcgctta cgacgacatt
     1861 ttcgccctgc gaaccttcaa acgtcaacgc cttcatgcga atgagatcta ccacgccgac
     1921 aaaatcgtcg ccggctccca tgttaatctg cacgggcacg acgttcaggc caagtttttc
     1981 aaccatgtcg gctctgacgc gcatggggtc ggcaccctgg cggtccattt tgtttatgaa
     2041 tgctatgcgg ggaactttgt agcgtttcat ctggcggtct acggtgacgg actgactttg
     2101 tacgccgccg acgctgcaga gaaccattat tgcaccgtcg agaacgcgaa ggctgcgttc
     2161 cacctcgacg gtgaaatcaa cgtggccggg cgtgtcgatg atgttgatgc cgttgtcttt
     2221 ccaatgtacc gacgtagcag ccgaggtgat ggtaatgccg cgttccttct ccagttccat
     2281 aaagtccatg gtcgcaccgt cgccgctgcc gtgtacttcc tgcatgcggt gaatgcggcc
     2341 ggtgtaaaaa agtatacgct cggtcaaggt cgttttgccg gagtcgatat gtgcggaaat
     2401 gcctatgttc cgaatcttgc cacgtcgttt cattttatct attccttctt cctattttta
     2461 agacaagtag tccagagcat ttcaaacttg atacttccgt ttttgctggt ttgctttgtt
     2521 cgtggctgcg tcagaatttt tttaggaata tcac
//
LOCUS       DAONFI010000330         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_53006_length_2535_cov_3.727823, whole genome shotgun sequence.
ACCESSION   DAONFI010000330 DAONFI010000000
VERSION     DAONFI010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53006_length_2535_cov_3.727823"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLK08_09385"
     CDS             <1..592
                     /locus_tag="PLK08_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HPS53753.1"
                     /translation="KESVFPFNRFPGVDAMLGPEMKSTGEVMGIDTDFCLAYAKSQQA
                     SGTKLPISGTVCISVKRADRAAMAPIVAKLKEMGFNILATEGTAKTLAENGVESKRIN
                     KVGQGQPHIVDAIKNGEIDLIINTPSGRRPRADEQAIRISAVNNGISLITTVTAASAC
                     VDSIERLRETHLAAVPIQNYLKDRINAVNLPVKPKK"
     gene            649..2241
                     /locus_tag="PLK08_09390"
     CDS             649..2241
                     /locus_tag="PLK08_09390"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPS53754.1"
                     /translation="MTEELHHECGVAALYWLNEAYNGSRDGQASELVVNDDVTPLMSP
                     LLLDLQNRGQLAAGISSYNPKRVQVLDTFKDVGRVSEVFRMSHPGKYKAIMEEYAGRA
                     VIGHTRYATCGADDSRYAQPFERHHGRAWKWFTFAFNGNLSNYRELRERLLSRRDYII
                     TLDTDTEIIMHMLAYQLRHDSPPDLAKVMSELAVDFDGAYNIVFLDAKGRMFVCRDPL
                     GLRPLCWAVQGKLFGVASESMALSNLGFDDIHSLEPGMMGIVEEGRLRFERFAPSTRK
                     AHCFFEWVYFSNVGSVQDDSSVYLSRSRAGKILAAKEDQNVKGDDVVVVPVPDTAKAA
                     ADSFAFALGIPCMEGLIRNRYVGRTFIQPGSSRGMTVRSKYTPQPALLKGRKVFLVDD
                     SIVRCTTMKALVKRLKEFGKAREVHVRVACPPIIAPCFYGIDMSTLGELFAHRYIDEN
                     YAGDLTPQQEKTMAAELGVDSLRYLSVDDLSASILMPKNHLCTGCVLGKYPTDYGNRL
                     MCAARKNDAEGKIGRTYESCNS"
     gene            2286..>2535
                     /locus_tag="PLK08_09395"
     CDS             2286..>2535
                     /locus_tag="PLK08_09395"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HPS53755.1"
                     /translation="MDIQLDTPVQYVKRVGPARAEQLAALNIHNVEDLIMHFPRRFDL
                     RRQAQPIASLNDGNEATVVGTVITAKLGGPRKKPSFSAT"
BASE COUNT          653 a          681 c          677 g          524 t
ORIGIN      
        1 caaggaatcg gtattcccgt tcaaccgctt ccctggcgtc gacgcaatgc tgggacctga
       61 aatgaaatcg acgggcgagg tcatgggcat agataccgat ttctgcctcg cctacgccaa
      121 gagccagcaa gcctccggca caaaactgcc catcagcggt acggtctgca tttccgtcaa
      181 acgagccgac agagccgcca tggcccccat cgtggccaag ctaaaggaaa tgggtttcaa
      241 catcctcgcg accgaaggca cagccaaaac gctggcggaa aacggcgtgg agtccaaacg
      301 tatcaacaag gtcggccagg gccagccgca tatcgtcgac gcgataaaaa acggcgaaat
      361 agacctgatt atcaatacgc cgtcaggccg ccggccaaga gcggacgaac aggcgatacg
      421 cataagcgcc gtcaacaacg gcatatcgct gataaccacc gtcaccgccg ccagcgcgtg
      481 cgtggacagc atcgagcggc tgcgggaaac gcatcttgct gccgtgccga ttcagaatta
      541 tctcaaagac agaataaacg cggttaatct gccggttaaa ccgaagaaat agaactgcgc
      601 atcaaggctg tttgttaaaa cggcttttta tgatataatc ggtgagttat gactgaagaa
      661 ttgcatcacg aatgtggcgt tgcagccctt tactggctga acgaagcgta taacggctca
      721 agggacggac aggcgtcgga actggtggtc aatgacgatg ttacgccgct gatgtcgccg
      781 ttgttgctgg acttgcagaa tcgcggccag cttgccgccg gcataagctc atataaccca
      841 aaacgggtac aggttctcga taccttcaag gacgtcggca gggtcagcga agtttttcgc
      901 atgtcgcacc ccggaaagta taaggcgata atggaggaat acgccggccg tgccgttatc
      961 ggccatacga gatacgcaac ctgcggcgcg gacgattccc gttacgcgca gccgttcgaa
     1021 cggcatcacg gcagagcatg gaaatggttt acattcgcat tcaacggcaa cctttcaaac
     1081 tatcgcgaat tgagagagag actcctcagc cgccgcgatt atataattac gcttgatacc
     1141 gatactgaaa tcatcatgca tatgctggct taccagctgc ggcacgattc cccgccggat
     1201 cttgccaagg tcatgagcga attagccgtc gattttgacg gcgcgtacaa tatagtcttt
     1261 ctcgatgcca agggacgtat gttcgtatgt cgcgacccgc tgggcctacg gccgttgtgc
     1321 tgggccgtcc agggaaaact tttcggcgtc gccagtgaat cgatggcatt gtccaatctc
     1381 ggttttgacg acattcattc gcttgaacca ggcatgatgg gtatagtaga agaaggcagg
     1441 ctgcgattcg agcgattcgc gccttcaacg cgcaaggcac actgcttttt tgaatgggta
     1501 tatttttcca acgtcggcag cgttcaggac gattcgagcg tttacctgtc gcgcagccgg
     1561 gcaggcaaaa tactcgcagc caaggaagac cagaacgtca aaggcgacga cgtagtcgtc
     1621 gtgccggttc ccgacaccgc gaaagccgct gcggattcat ttgcattcgc attgggcatt
     1681 ccctgcatgg aaggccttat acgcaatcgt tacgtcggca ggacgttcat tcagccaggt
     1741 tcatcgcgcg gcatgacagt acgcagcaag tacacgccac agccggctct gctgaaaggc
     1801 agaaaagttt tcctggtaga cgattcaata gtgcggtgca cgaccatgaa ggcgctggtc
     1861 aaacggctca aggaattcgg caaagccagg gaagtgcacg tccgcgtggc atgtcctccg
     1921 attatagcgc cgtgttttta cggcattgac atgtcgactc tcggtgagct ttttgcgcac
     1981 agatacatag acgaaaacta tgcgggtgac ctcacgccgc agcaggaaaa aaccatggct
     2041 gccgaactgg gcgtggattc gctgcgatat ctcagcgtgg acgacctttc ggccagcata
     2101 ttgatgccca aaaatcatct ttgcaccggc tgtgttttgg gcaaatatcc aacagactac
     2161 ggcaatcgcc tcatgtgtgc cgccaggaaa aacgacgccg aaggtaaaat cggcagaacc
     2221 tacgaaagct gcaattccta gaaaaatttt gcgccgttaa aaactcacga tttttaattt
     2281 ttaatatgga cattcaactc gatacgccgg tgcaatatgt aaaacgggtc ggcccagcca
     2341 gagccgagca acttgcggca ttgaatattc acaacgtcga agatctcata atgcacttcc
     2401 ctcgcagatt cgatcttcgc aggcaggcac agccgatagc atcgctcaac gacggcaatg
     2461 aagcaacggt agtcggcacg gtaataaccg ccaaactcgg cggcccaagg aaaaagccca
     2521 gcttttcggc tacca
//
LOCUS       DAONFI010000331         2518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_53546_length_2518_cov_3.193260, whole genome shotgun sequence.
ACCESSION   DAONFI010000331 DAONFI010000000
VERSION     DAONFI010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53546_length_2518_cov_3.193260"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /gene="dnaG"
                     /locus_tag="PLK08_09400"
     CDS             <1..1628
                     /gene="dnaG"
                     /locus_tag="PLK08_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPS53756.1"
                     /translation="CFACGAGGDAIGFVMKYEKLSFMEAVRMLAEQAGMAMPQLDHQN
                     FAPAQPGMGKNDLAAVTEFAARFYQNQLGSPSGQGALAYAHSRGLTDETIQKFRIGFA
                     PDSWDAFITAARQQGFAEQQLFAAGMMRQRENSSGGYDWFRNRLMFPIRDLGGRVIGF
                     GGRALADGENAKYLNSPESVLFDKSSLVFDMSLARESIVASRQAVVVEGYMDVIVPHQ
                     AGVTNLVATLGTALTEQHVRLLARYAEEVVLMFDADTAGAAAAQRALELFIRQQFNVR
                     VATIPSGKDPCDMVLAQGPDALRSLIAGAPDALQYVWDRRYEQYSHADNPAARRKIIE
                     DFLQLIASSAAYGAVDAVRQQNISQHIAHLLNMSAIDVNGQIRRLVRKIKPRGLAAQP
                     QTPSQSNSTMSLSPERQILEVLLDKPELFDEIVERIDPPHFEDPLCRAIAEQIWHIGV
                     AGKFTLESLMAQPQFTEWQGLLAELSFAGQRRGNHSATLNDAVNKILQREHYDELTQL
                     KNNLSDDDSLRSLGEKLSHAAKQTNRLKRPRIF"
     gene            1797..1869
                     /locus_tag="PLK08_09405"
     tRNA            1797..1869
                     /locus_tag="PLK08_09405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1830..1832,aa:Gly,seq:tcc)
     gene            2318..>2518
                     /locus_tag="PLK08_09410"
     CDS             2318..>2518
                     /locus_tag="PLK08_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53757.1"
                     /translation="MTFYPERNSDQKSFEIAIEAYKKQISIAAKAVAVFSENIKSLGN
                     RRFGTYTLTRNSNFSVNHQLYNF"
BASE COUNT          606 a          586 c          647 g          679 t
ORIGIN      
        1 aatgcttcgc ctgcggtgcc ggcggtgatg ccatcggttt tgtgatgaaa tacgaaaagc
       61 tctcgtttat ggaggcagtt cgtatgcttg ccgagcaggc tggcatggct atgccccagc
      121 ttgaccatca gaattttgct ccggctcagc cgggaatggg caaaaacgac ctggcagccg
      181 ttactgaatt tgccgcgcga ttttatcaga atcagcttgg cagtccgtcg ggacaggggg
      241 cgctggctta tgcccattcc cgcggtttga ccgatgagac catacaaaaa ttccgcattg
      301 gttttgcgcc cgattcatgg gatgcgttca taaccgctgc gaggcagcag ggttttgccg
      361 aacagcagtt atttgccgcc ggcatgatgc ggcagcgtga aaattcgtct ggcggttatg
      421 actggtttcg taatcgcctg atgtttccca tacgcgacct cggcggccgt gttatcggtt
      481 ttggcggcag ggcgctggct gacggcgaaa acgccaaata tctcaacagc cccgaaagcg
      541 tgctgttcga taagtcgtcg ctggtattcg acatgagcct ggcacgcgaa agtatcgttg
      601 ccagccgaca agccgtagtc gtcgaaggct atatggacgt gattgttccg catcaagccg
      661 gcgtgacgaa cctcgttgcg acattgggca cagccctgac cgaacagcat gtccgtctgc
      721 tggctcgcta tgccgaggaa gtcgttctga tgttcgacgc cgacaccgcc ggcgcggctg
      781 ctgcccagcg tgcgttggaa ctttttatcc ggcaacagtt caatgtgcgg gtggcgacga
      841 taccgtccgg taaagatccg tgtgatatgg ttctggcgca gggccctgat gcgctgcggt
      901 cgcttattgc cggcgcaccc gacgcactgc aatacgtctg ggacagacgt tacgagcaat
      961 attcgcatgc cgacaatcct gccgcgcggc gaaagattat tgaagatttt ttgcagttga
     1021 tagccagctc ggcggcttat ggtgctgttg atgcggtacg gcagcagaat atttcgcagc
     1081 atattgcgca tttgctgaat atgtcggcga tagacgtaaa tggtcagatt cgcaggctgg
     1141 tcaggaaaat caagccgcgc ggcctggctg cacagccgca aacgccttcg caatcaaaca
     1201 gtaccatgtc actcagcccc gaacggcaga tactcgaagt tttgctggat aagccggaac
     1261 tttttgatga aatcgttgag cgtatagatc cgccgcattt tgaagatccg ctctgccgcg
     1321 caattgccga gcagatttgg catatcggcg tggctggcaa gtttacgttg gaaagcctta
     1381 tggctcagcc gcagtttact gaatggcagg gccttcttgc ggagctttcc tttgctggcc
     1441 aacggcgggg caatcattcc gccacgttaa acgacgcggt aaataaaatt cttcagcggg
     1501 aacattatga tgaactgacg cagttgaaga ataatctgtc tgatgacgac tcgctccgca
     1561 gccttggcga aaaactctcc catgccgcca aacagaccaa ccgcctcaaa cggccgcgaa
     1621 tcttttgatt ttgtgaatga aataatggag atagttggct gaatattggt taggcgagaa
     1681 tctttcccaa tgatttttta ttataaaaca tttcacattg ggatttttgt atcataatct
     1741 tgacaaatca ttttagatat ggaagatacg ctttattgaa ttggctaatt ttgaatgcgg
     1801 gcgtaactca atggctagag tgtcagcctt ccaagctgaa tgttgagggt tcgagtccct
     1861 tcgcccgctt atctgaattt aactgtaagt ctttattctc aaaaggagtt acagttttct
     1921 ttttatttcc atcaaaaacg cccgacttgc taaaatttac caaactttta catatcttat
     1981 cagagtttcg agtcgaataa cgggtagaat tttctgcccc gtccgtaccg gttatagcta
     2041 acgcttgatg aagctcaaat tccggcagct tggcaatagc tgaagcctta tcttccatgc
     2101 tcaaatgcgt gtaataagcc agagtcaagt ccatgtttga atgcctcatc aagcgagttg
     2161 ctgtacgtct tcttacaaaa tggtagtttt aaggccgtta attaagagag actttttgct
     2221 gttggatttt cctaatccgt aaccgtttac gggaatatgc tatgagttga gggtatggtg
     2281 tgcctgtaag ggttgagttg acggcagcct tgaaataatg acattttatc ctgaaagaaa
     2341 ttccgaccag aagtcttttg aaatcgccat tgaagcttac aagaagcaaa tttctatagc
     2401 tgctaaagcg gtggcggtgt ttagtgaaaa tataaagagc ttagggaatc gtcgctttgg
     2461 tacttatact ttaacaagaa attccaattt ctcagttaat catcaactct acaatttt
//
LOCUS       DAONFI010000332         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_53576_length_2517_cov_3.881397, whole genome shotgun sequence.
ACCESSION   DAONFI010000332 DAONFI010000000
VERSION     DAONFI010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53576_length_2517_cov_3.881397"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /gene="secG"
                     /locus_tag="PLK08_09415"
     CDS             <1..574
                     /gene="secG"
                     /locus_tag="PLK08_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPS53758.1"
                     /translation="VVLLQKGRGGGLGAAFGAGAGSAFGTKTGDVLTWVTIALTVLFL
                     GLTLLSVVAIKPTKMPSEMPAFNPEHPITERTFVQITCPTAKAAIHFTTDGSEPNEKS
                     SKYNNSAIRIRPGQTLKAIAYATGMTPSPVKSYTYLTPEDAARQEAEKAQKAAEEKAE
                     TTTKKLPTDTTPEIPVLPAAPATDKPAKSK"
     gene            617..1498
                     /locus_tag="PLK08_09420"
     CDS             617..1498
                     /locus_tag="PLK08_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPS53759.1"
                     /translation="MLTSMTGFGSVAGKVDTVEYSIEIKSVNNRYLKLSTRMPDGLHM
                     LEPQIEALLRQKIQRGSVTLCIRMKIADENAASTINTAAINSYMKQLQMLEVDANPMM
                     RVDLATLLTLPGVCTPPALDELAEKTSQGLLCLIDRVIDILIAMRNTEGAALAKDLLS
                     QTAVISEKLESIASRTDEVLQLYHSKLKARVEELTATAKIAINEDSLAREVALFAERS
                     DIAEEITRLRTHMTEFVKVVNAGGPVGRKLDFIAQEMLREANTIASKSSDADIARLVV
                     DIKTAVDRIKEQAANVE"
     gene            1491..2054
                     /gene="gmk"
                     /locus_tag="PLK08_09425"
     CDS             1491..2054
                     /gene="gmk"
                     /locus_tag="PLK08_09425"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPS53760.1"
                     /translation="MSEIKGKLVVVSGPSGVGKSTITRRAMRLVGAVYSVSATTRPPR
                     PGETDGVDYRFVTRNEFEKMAAADELLEWAEVFGNFYGTPAEPVRRLIKQGKTVVLEI
                     DVQGGLQVFKKCPDACFILVVPPSEESLRQRLIGRGTEDAISIEKRFGKAKEELQTAR
                     RSGAYTHVVVNDDLDEAVNEVARIMGE"
     gene            2071..2304
                     /locus_tag="PLK08_09430"
     CDS             2071..2304
                     /locus_tag="PLK08_09430"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPS53761.1"
                     /translation="MIEALKSEEIVNKVGGRFKLTALIQRRIVELMQGSRPLVERKPG
                     MTDMELAIEEILADKLAIDYENSDVPSPAELEA"
     gene            2309..>2517
                     /locus_tag="PLK08_09435"
     CDS             2309..>2517
                     /locus_tag="PLK08_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HPS53762.1"
                     /translation="MTARPGKKLADYEILLCVTGGISAYKAAELVSRFVQADAGVTVA
                     MTENAQKFVGPSTFQALTRRQVFTS"
BASE COUNT          673 a          647 c          710 g          487 t
ORIGIN      
        1 ggtcgtattg cttcagaagg gccgcggcgg cgggcttggc gccgcgttcg gcgcaggcgc
       61 gggcagtgcg ttcggcacaa aaaccggcga cgtgctgacg tgggtgacaa tagcactgac
      121 cgtgctgttt ctcggcctga cgcttctttc cgtagtcgcc atcaagccga ccaaaatgcc
      181 ctcagaaatg ccggcgttca atcccgaaca tccgataacg gaacgaacgt ttgtacagat
      241 tacctgtccg actgccaagg cagccataca cttcacgacc gacggcagtg agcccaacga
      301 aaagtcgtcg aagtataaca attccgccat tcgtatcagg cccggccaga cgcttaaagc
      361 catcgcctat gcaaccggca tgactccctc gcccgtcaag agctatacgt atcttacgcc
      421 ggaggacgcc gccaggcagg aagccgaaaa agctcaaaaa gctgccgaag aaaaggctga
      481 aactacgaca aaaaaactcc caaccgacac aacgccagaa atacccgtct tgcctgcggc
      541 acctgccaca gacaagcctg caaaatcaaa atagcgaata ttttaatatt tttacacaca
      601 gcggaatgga tttatcatgt tgacgtcaat gacaggtttc ggctcggtag ccggcaaagt
      661 ggacacggtg gaatattcca tcgaaatcaa atcggtgaac aaccgctatt tgaaactgtc
      721 cacgcgcatg cccgatggcc tgcatatgct tgaaccgcaa atagaagccc tgttgcgaca
      781 gaaaatccag cgtggcagcg ttacgctgtg catacggatg aaaatagccg acgaaaacgc
      841 cgccagtacc atcaacaccg ccgcaatcaa cagctatatg aaacagttgc aaatgctgga
      901 ggtggatgca aatccgatga tgcgtgtcga ccttgcgacg cttctgacgc tgccaggcgt
      961 ctgcacgccg cctgcgcttg acgagttggc ggaaaagacg tcgcagggcc tgctgtgcct
     1021 gatagacaga gttatcgaca tcctgatagc catgcgaaat actgaaggcg ccgcactggc
     1081 aaaagacctt ctcagccaga cggctgtgat atccgaaaaa cttgaatcaa tcgccagccg
     1141 aacggatgaa gtgctgcagc tttatcactc gaaactcaaa gcgcgtgttg aggagttgac
     1201 ggcaacggct aaaatagcga taaatgaaga ctcgctagcg agagaagtgg cgttattcgc
     1261 cgagcgtagc gacatagccg aagaaatcac ccgtctgcgt acgcatatga cggaattcgt
     1321 caaggtcgtc aacgccggcg ggccggtcgg ccgaaagctg gacttcatcg cccaggagat
     1381 gctgcgggag gctaatacca tagcgtccaa atcttccgat gccgacattg cgcggctggt
     1441 ggtggacata aagacggctg tagaccgcat caaggagcag gcggccaacg ttgagtgaga
     1501 ttaagggaaa actggttgtc gtcagcggcc cgtcaggggt cggcaagagt acgataaccc
     1561 gccgggccat gcggctcgtc ggagcggttt acagcgtatc ggcaacgaca cgtccgccgc
     1621 ggccgggaga aacggatggc gtagattatc gcttcgtgac gcgaaacgaa tttgaaaaaa
     1681 tggctgccgc cgatgaattg ttggaatggg cggaagtttt cggcaatttt tatggcacgc
     1741 cggcagaacc ggtacgccgg ctgataaagc agggcaagac ggtcgttctg gaaatagacg
     1801 tccagggcgg cttgcaggtt tttaaaaaat gtcccgatgc ctgctttatt ctggtcgtgc
     1861 cgcccagtga ggagtcgttg cgacagcggc tcatcgggcg aggcacggaa gacgcgatat
     1921 cgatagaaaa acgcttcggc aaggcaaaag aagaattgca gacagcccgc cgcagcggag
     1981 catatacaca tgtggtcgtc aacgatgacc ttgatgaagc agtaaatgaa gttgcccgta
     2041 ttatgggcga ataacacgga gataatacag atgattgaag cactcaagag cgaagaaatc
     2101 gtcaacaagg tcggcggcag gttcaaactt accgcattga ttcagaggcg tattgttgaa
     2161 ctcatgcagg gttcgcggcc gctggtggaa cgtaaaccag gcatgacgga tatggaactt
     2221 gccatcgagg aaatacttgc cgacaagtta gccatcgatt acgagaacag cgatgttccg
     2281 tcgccggctg aattagaggc ctgatgtgat gaccgccagg ccgggcaaaa aacttgccga
     2341 ctatgaaata ctgctttgcg taacgggcgg tatttccgct tataaagctg cggaacttgt
     2401 tagccgtttt gtgcaggctg acgccggcgt aaccgtggcc atgacggaaa atgcgcaaaa
     2461 atttgtaggg ccgtcgacat ttcaggctct cacccgcagg caggttttca cgagcat
//
LOCUS       DAONFI010000333         2511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_53768_length_2511_cov_4.436889, whole genome shotgun sequence.
ACCESSION   DAONFI010000333 DAONFI010000000
VERSION     DAONFI010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53768_length_2511_cov_4.436889"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1027
                     /locus_tag="PLK08_09440"
     CDS             113..1027
                     /locus_tag="PLK08_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPS53763.1"
                     /translation="MINYGIIVGLLSALLQSVSYLFARKFSSEYQNSNIRLLVSSQIL
                     MGIAGLFALPFCWPAHIDWNWSWIQPVILCVFFSLAGQLLIFFALYSAEASKISPLLA
                     LKVVMIAFLTWWMMGIPLNYLHWLGVLLCLVGTFILNFSGGGISRRAIIGVLGACLFY
                     SLSDINITILVENLESQMSSRWGAVFFSVCAIYILCGVLCAFCMFFVKEAKWSDLKGS
                     AVGFSLAWLIAMVFMFATFSIVGTVYGNILQSMRGIISIFLGVLIASKGHQHIEAHMP
                     RKVFLRRLIAALIMTAAIAAFGAGGLHA"
     gene            1645..>2511
                     /locus_tag="PLK08_09445"
     CDS             1645..>2511
                     /locus_tag="PLK08_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPS53764.1"
                     /translation="MEHSTTESTESNQKNLPVFRIDEQQVQNHLGKMVRENVEQFINS
                     MLDAEADELCKASRYERSPERIDTRAGHYKRKLHVAAGEVTLKVPKLRSLPFETQIIE
                     RYRRRESSVEESLVEMYLAGVSVRRVEDITEALWGTRVKPGVVSSLNQKIYERIEAWR
                     NAPLTNSHPYVYLDGIWLKRSWGGEVKNIAILVAVGVNDDGFREILGVCEGCKEDEQS
                     WLAFLRHLKKRGLNGVRLVISDKCLGLVEALGEVFPNTDWQRCTVHWYRNVLSKVPSD
                     RKKEGAAILKAIH"
BASE COUNT          660 a          512 c          656 g          683 t
ORIGIN      
        1 caaagacatc ctcgtaaaat gaagatacat accatatatt gaaaagtgtt cagatatttt
       61 tgtgtttttg aaaaggatat ttattgccct tgctggcgag taagtgtgga aaatgataaa
      121 ttatggaata atcgtaggtt tgctgtcggc tttgttacaa tcggtatcgt acctttttgc
      181 tcgcaagttt tcatcagagt atcagaactc aaatatacgt ttgttggtct ccagccaaat
      241 tcttatggga attgccggct tgtttgcact gccgttctgt tggcctgcgc atattgactg
      301 gaattggagc tggatacagc ctgtaatact gtgcgtgttt ttttctctcg ccggacaact
      361 gctgatattc tttgccctgt attccgccga agcgtcaaaa atttcaccgc ttttggcatt
      421 aaaagtggtt atgattgcgt tcctgacatg gtggatgatg ggtattccgt tgaattatct
      481 gcattggctg ggagtattgc tatgcttggt cggtacgttt atattgaatt tcagcggagg
      541 cggaataagc cgtcgagcca taatcggcgt acttggcgcc tgcctgtttt attcattgtc
      601 ggacataaac ataacaatat tggtcgaaaa tctcgaaagt caaatgtcaa gccgttgggg
      661 tgcagttttt ttcagtgtgt gcgcaatata tattctgtgc ggagtattat gtgcattttg
      721 catgttcttt gtaaaggaag caaaatggtc tgatctaaag ggttcggctg ttggattttc
      781 attggcatgg ctgatagcca tggtttttat gttcgccact ttttcaattg tcggaacagt
      841 ttatgggaac atattacaat caatgcgtgg aataatttca atctttctcg gtgtgctgat
      901 agccagcaag ggtcatcagc atattgaagc tcacatgcca cgcaaagtct ttctccggcg
      961 attgattgca gctctgataa tgacggctgc gatagctgct tttggagcag gaggattaca
     1021 tgcataatat attgaaaatt actcaaactg cccgattaag ccgcaaggag tttttccaag
     1081 cggtggatga ctgattttca tgcgatactt ttatgacatt taaaaatttt cacaatgagg
     1141 ggccaggcag ccataggcgt catcaacaag gcgaagtgtc tttcaatcac gctgtaccat
     1201 cgcacgcagg agtacatcaa aacgcgagtt aatgaaattt ctattgaccc cgctgtaaaa
     1261 aggtgaattt ctctgggatc tgagggctat ttcaaattgc atctgcctcg atattcgata
     1321 ttatatccat cttgaaggga ctctaaccct tggtagctcg caatgaaaaa ggtaccgggt
     1381 ggatggggta ctaatcatcg aaaaaggggc cacggataat tgaaaattcg taagatgttc
     1441 ttatttttta gagaaggata ggtacccgcg tgaaccggcg gctgtccgcg tagcaatcgt
     1501 gcggctgtgg gttcgaatgg ccatgtctat tgataacgta tactattttt cgcacatttg
     1561 ggctgaggat gtagacaagc agtctccagg ttggtggaat gtagattgat ttcgataaac
     1621 aattcactaa cacaggagac tgcaatggaa catagtacta cagaatcgac tgaaagcaac
     1681 caaaagaatc tgccggtctt tcgaatcgac gagcagcagg tacaaaacca cctcggcaaa
     1741 atggtccgcg agaatgtcga gcagttcatt aacagcatgc tcgatgccga ggctgacgag
     1801 ctttgcaagg ccagcaggta tgaacgaagt cctgaacgaa tcgacacgcg ggccggccac
     1861 tataagcgga agctgcacgt ggcggcaggc gaagttacgc tcaaagttcc caagctccgt
     1921 agtctgccgt tcgagacgca gataatcgaa cgctatcgcc gccgtgaaag cagcgtcgaa
     1981 gaatcgctcg tggagatgta ccttgccggc gtaagcgtgc ggcgtgttga agacatcacg
     2041 gaggcccttt ggggcactcg ggtaaaaccc ggcgtggtca gcagcctgaa ccagaagatt
     2101 tatgaacgca tagaggcatg gcggaacgca ccgttaacga atagccatcc gtatgtttac
     2161 ctcgatggaa tatggctaaa acgcagttgg ggcggcgagg taaaaaacat cgcgatactc
     2221 gttgccgtcg gtgtgaatga cgacggattc cgtgagatat tgggcgtatg cgaaggctgc
     2281 aaggaagacg agcagtcgtg gctggcattc ctgcgacact tgaagaaacg cggattgaac
     2341 ggggtgcggc ttgtaataag cgacaagtgc ctcggccttg tcgaagcgtt gggcgaagtt
     2401 tttcctaata ccgattggca acgctgcacg gttcattggt atcgtaacgt gttatcgaaa
     2461 gtgccgtcgg acagaaagaa ggaaggagcc gcgattctca aggccatcca t
//
LOCUS       DAONFI010000334         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_54647_length_2484_cov_3.988884, whole genome shotgun sequence.
ACCESSION   DAONFI010000334 DAONFI010000000
VERSION     DAONFI010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54647_length_2484_cov_3.988884"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="PLK08_09450"
     CDS             complement(<1..1587)
                     /locus_tag="PLK08_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53765.1"
                     /translation="MMAVTNLKKLPNVKRVKSRPVVFGVCATGDPRVDAASRERCVNI
                     IEMVATAIAKNVVMPCGTPVDVVWSPVLIDGEKEADVVARQFRDAGVNAVVIAPDTWA
                     FPQLTILSLLSQLPQDMPVNITCGNSGPKPGVVFAQAINGALAQCGRLSAMNVGTWPD
                     TGINPQMTESTAKSLIDWCYAALTFAGLKGRRVLVFGHDSMGMETALAHVLPTRRTFG
                     LEITRLDMKLLADMLRKGSYSEKELAELRGWIDKNIGSRLTLRNEKDSQAFNDSLAMY
                     LIVRDLMAELNAVGGGFMSQLEWGSDRRGIPMPVADAMESIFNSNFDHNGSKTVMPFA
                     TEADVQGLLTMLFFTFLSGGNPPLFMDFRKVWEPWEIQAMAKKMKIGFSTKDVWAMRG
                     FVDGNNSGSASFNWAGRPGETPATLMKRISMPLAEDFYFPGGGNSVAFFTPGGIEGIA
                     GRMAYCAVNDRFSLIWDEAITTEMPEKLSQAIGKTSNITWPHTFIVPKYADMIEYKQY
                     APANHFHMTWNLPVARLQHWM"
     gene            complement(1883..2281)
                     /locus_tag="PLK08_09455"
     CDS             complement(1883..2281)
                     /locus_tag="PLK08_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53766.1"
                     /translation="MTLFVIILSLLVIATVMSLPGTLVVKIGSRLMGHFTTSFHTAYT
                     AVVSSYLVYFAIQVVFNMFFKMRLSVGVLLGMMYLLHAWTYFRRVRSDEGKVLNIPAS
                     LIMALFQVLITGLVIVFIIKLPLGMSYSDT"
BASE COUNT          633 a          673 c          579 g          599 t
ORIGIN      
        1 catccagtgc tgcagccgcg ccaccggcaa attccacgtc atgtgaaaat gattcgccgg
       61 agcgtattgc ttgtattcta tcatgtccgc atattttgga actataaaag tatgcggcca
      121 tgtgatattt gacgttttgc cgatagcctg cgaaagtttt tccggcattt cagtggtaat
      181 cgcctcgtcc catatcaggc tgaacctgtc gtttacggca cagtacgcca tgcggcctgc
      241 gatgccctct atgccgcccg gcgtaaaaaa tgcgacgctg ttgccgccgc cgggaaaata
      301 aaaatcttca gccagcggca ttgaaatgcg tttcataaga gttgccggcg tttcgccagg
      361 ccggccagcc cagttaaagc tggctgaacc gctgttattg ccgtccacga aaccccgcat
      421 cgcccaaacg tctttggtgg agaatccgat tttcattttt ttggccatcg cctgtatttc
      481 ccacggctcc cagaccttgc ggaaatccat gaacagcggc ggattgccgc cggaaagaaa
      541 tgtgaaaaac agcatcgtca gaaggccctg cacgtcagcc tccgtggcga aaggcatgac
      601 cgtctttgag ccgttgtggt cgaagttgga attgaaaatc gactccattg cgtcggcgac
      661 cggcatgggt atgccgcgac ggtcgctgcc ccattcaagc tggctcatga agccgccgcc
      721 aaccgcattc agctcagcca tcaggtcgcg cactatcaga tacatcgcca gcgaatcatt
      781 gaatgcctgc gaatcttttt cattccgcaa cgtaagacgg ctgccgatat ttttgtcaat
      841 ccagccgcgc agctctgcga gttccttctc cgagtatgaa cctttcctga gcatatcggc
      901 aagaagtttc atgtctaacc gcgttatctc aagcccgaaa gtgcggcgag tcggcagaac
      961 gtgtgcgagg gcggtctcca tacccataga atcgtggccg aagaccaaca cacgccggcc
     1021 ctttagccct gcgaaggtaa gagccgcata acaccaatca ataagcgatt tggcggtcga
     1081 ctccgtcatc tgcgggttta tgcccgtatc cggccatgtg ccgacgttca tcgccgacag
     1141 cctgccgcat tgcgccagcg cgccattgat agcctgcgca aaaaccacgc ccggcttggg
     1201 cccgctgttg ccgcaggtga tattgacggg catgtcctgc ggcagttgcg atagcaagct
     1261 gagaatcgta agctgtggaa acgcccacgt atccggcgct atgaccacgg cattcacgcc
     1321 ggcgtcacgg aactgacgtg caacgacgtc agcctctttt tcgccgtcga taagcaccgg
     1381 agaccatacc acgtcgaccg gcgtgccgca gggcataacc acgtttttgg ctatcgcagt
     1441 agcgaccatt tcaattatat tgacgcaacg ttcacggctg gcggcatcca cgcggggatc
     1501 gccggtcgca caaacgccga aaaccacagg cctgctcttg acgcgcttga cgttgggaag
     1561 ttttttaaga ttggtgacgg ccatcatatt ctcccttcaa aaggattttt cctgaaaata
     1621 aagtgattgc acagcatgga acgattctca aaatattttt ctttcgcgaa ccactccaga
     1681 gcattttaaa gttgatattg cgacatacct gctaccgcgg ctacggcggg caacgcgtta
     1741 agatacaatc tcaacgttga gatgctctaa aaacattatc gcagttaacc tttttacggt
     1801 aactgcaact ttgcgcattt ttttcaagaa tttgcgcaaa cagtaattgt ctccattttc
     1861 gcatttgccg gccgtctcag tcttacgtgt ctgaataact cattcccagc ggaagtttta
     1921 ttatgaacac gatgacaagt cccgttatca acacctgaaa taacgccata ataagcgacg
     1981 ccggaatatt taatactttc ccctcgtcac ttctgacgcg gcgaaaatac gtccacgcat
     2041 gaagcaggta catcattcca agcagaacgc caacgctgag tcgcatttta aaaaacatgt
     2101 tgaaaacgac ctgaattgcg aaatatacga gatatgatga aactaccgcg gtatacgccg
     2161 tgtgaaaaga cgtggtaaaa tggcccatca gccgcgagcc gattttgacg accaacgtcc
     2221 ccggcagtga catgaccgta gctatcacca gcaatgacaa tataattaca aatagggtca
     2281 tagaagcatt cccgcatgaa caaaatccat taaattatag cccttttttt tcaaaaaact
     2341 ccggttatgt agaattttct tgtccattta cggctaaaat tgtaatatgc ccgcgtcatg
     2401 tttaatatgg agctacaaaa gacatattaa aaaagtgaat ctctacgggt taaaacccgt
     2461 agcatcatat tcatcaggct ttcg
//
LOCUS       DAONFI010000335         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_55124_length_2470_cov_4.307660, whole genome shotgun sequence.
ACCESSION   DAONFI010000335 DAONFI010000000
VERSION     DAONFI010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55124_length_2470_cov_4.307660"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLK08_09460"
     CDS             <1..688
                     /locus_tag="PLK08_09460"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPS53767.1"
                     /translation="LFADLEQVTTRIEKLEVSTKKPTPKRDEQLRELELMKRMRDALE
                     NDRPISEVFSNDAEEKLVRSFGFLSQKPALVVINCSEDQAADDSSEPVAGLPSLKLSA
                     QIEQEIAQLPAEDRGEFLKDLGLSASVADRFIRACYMGMNLVSFLTAGDDECRAWTIA
                     KGDDAVEAAGKIHSDIARGFIRAETVAFDDFKAAGGSMKDVKAAGKMRLEGKTYVVQD
                     GDIINFRFNV"
     gene            642..1877
                     /gene="dinB"
                     /locus_tag="PLK08_09465"
     CDS             642..1877
                     /gene="dinB"
                     /locus_tag="PLK08_09465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPS53768.1"
                     /translation="MSFRTETSSTSGSMSDIRRIIHVDMDEFFAAVEKLDNPELRGRP
                     LLIGGSPRSRGVVSTASYEARAFGCHSAMPMATAIRLCPQAVVLPVRGSRYSQVSDQV
                     FEIFERFTPLVEPLSCDEAFLDVTGSVRLFGGGEKIGRRIKQTIRDELGLVASVGVAP
                     NKFLAKLASDLDKPDGFVVLRPQTYQSVIDSLPLTKLWGVGPSTLKTFERYNLKTIGQ
                     LRMLSQKDVAEWFGDGGEFFWRLANGLDDRPVVPDHQAKSIGQEQTFASDVADVVELR
                     RVLNDHVERITRRLRRHELLAKTITLKLRYGDFVTVTRSVSLKVPADTTDDFLKLAND
                     IFDRWCDDGFKPLRLIGATLSNLRHRQGRQMSLFGEAEEQKKRRLDSVIDDIADKFGS
                     DVLRRGGNILKNHRDESDE"
     gene            complement(1909..2388)
                     /gene="rpiB"
                     /locus_tag="PLK08_09470"
     CDS             complement(1909..2388)
                     /gene="rpiB"
                     /locus_tag="PLK08_09470"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPS53769.1"
                     /translation="MKIAVGSDHRGHGAKEKIMVLLGELGHEVQDVGTHNKKSCDYPD
                     SAYAAAMSVSKGESDCGILICGSGIGVSICANKVKGIRAALCHDELTAELAKRHNQAN
                     ILCLASDVLGDEIIRRIVTCWLNTEAENSPRHIRRIQKITMIEQGVDPREYQTQQEE"
BASE COUNT          552 a          630 c          703 g          585 t
ORIGIN      
        1 gctctttgcc gacctggaac aggttacgac gcgaatagaa aagctggaag tttcgaccaa
       61 aaagcccacg ccgaaacgtg acgaacaact ccgcgaactt gagctgatga agcgcatgcg
      121 cgacgccctt gaaaacgacc ggccgattag cgaggttttt tccaacgatg ctgaagaaaa
      181 gctcgttcgc agtttcggtt ttctgtctca aaaaccggca ctggttgtca ttaactgtag
      241 tgaagaccag gcggccgacg attcatcaga gccggtagcc ggtttgcctt cgctgaagct
      301 cagcgctcag attgagcagg aaatcgccca gcttcctgct gaagacaggg gcgagttttt
      361 gaaggatctc ggcttgagtg cgtcagttgc cgacaggttc attcgtgcgt gttacatggg
      421 catgaacctt gtgagcttcc tgaccgccgg tgacgacgag tgccgcgcct ggacgattgc
      481 caaaggcgac gatgctgtcg aggctgcggg caagattcac tccgacatcg cccgcgggtt
      541 catacgtgcc gaaacagtcg cctttgacga tttcaaagcc gccggcggca gcatgaaaga
      601 cgtcaaagcc gccggcaaaa tgcggcttga gggcaaaacc tatgtcgttc aggacggaga
      661 catcatcaac ttccggttca atgtctgaca tcaggcggat aatccatgtc gacatggacg
      721 agtttttcgc agccgtggaa aagctcgaca atccggagct tcgcggcagg ccgctgctta
      781 tcggcggttc gcccaggtca cgcggcgtag tcagtacggc aagctatgaa gcccgcgcgt
      841 tcggctgtca ttccgcaatg ccgatggcta cggcgatacg gctgtgcccg caggctgtcg
      901 tcctgccggt gcgcggcagc cgttattcgc aagttagcga ccaggtcttt gaaatattcg
      961 agcgtttcac gccactggtt gaaccgctta gctgcgacga agcgtttctg gacgtgaccg
     1021 gcagtgtacg gctgttcggc ggcggggaga aaatcggcag acgcataaaa cagacgatac
     1081 gcgacgaact tggcctggtg gcatcggtgg gtgtggcgcc gaacaagttt ctggccaagc
     1141 tcgcgagcga tttggacaag ccggacggct ttgtcgtact taggcctcaa acatatcagt
     1201 cggttataga ttctctgcct ttgactaaac tctggggcgt tggcccgtca acgcttaaaa
     1261 ctttcgagcg gtacaacctc aaaacgatag gccaactgcg aatgctgtcg cagaaagatg
     1321 tcgctgaatg gtttggcgac ggcggcgagt ttttctggcg gttggctaat ggccttgacg
     1381 ataggcctgt cgtaccggac catcaggcca aaagcatcgg ccaggaacag acattcgcca
     1441 gtgacgtggc tgatgtcgta gaattgcgac gcgtcttgaa cgatcatgtg gaacggataa
     1501 caaggcggct gagacgacat gaactgctgg caaaaaccat taccctgaag ctgcgatacg
     1561 gagatttcgt gacggtaacg cggtcggttt cgctaaaagt gccggctgat actacggatg
     1621 acttcctgaa attggcgaac gatatttttg acagatggtg tgacgacggt tttaagccgc
     1681 tgcggctgat aggggcgaca ctgagcaatc tgaggcacag gcaggggcgg cagatgtcgc
     1741 ttttcggcga agcggaggaa cagaaaaaaa ggcggcttga ttcggttatc gacgatatcg
     1801 ccgataaatt cggctcagat gtgctcagaa gaggcggaaa tatcttaaaa aatcatcgcg
     1861 acgaaagcga cgagtgaata gaccaaccgg ctggcagatg cactttgctt attcttcctg
     1921 ctgggtctgg tattctctgg ggtctacgcc ctgttctatc attgtgattt tttgaattcg
     1981 ccgtatatgc ctgggagagt tttccgcctc agtattcagc cagcatgtga ctattctgcg
     2041 aattatctcg tcgcccaata cgtcgctggc caggcagagt atgttcgcct gattatgccg
     2101 tttagccaat tctgccgtaa gttcgtcatg acagagcgcg gctcgaatgc ctttgacctt
     2161 gttggcgcat atgctcaccc ctatgccgct gccgcagata agtatgccgc agtcgctttc
     2221 gcctttcgat accgacatgg cggcggcgta tgccgagtcg ggatagtcgc agcttttctt
     2281 gttgtgtgtg cctacgtcct gcacttcatg tccaagctcg ccaagcagca ccattatttt
     2341 ttccttggcg ccgtggcctc tgtggtccga accgactgcg attttcattt tcacttcctg
     2401 caatttataa ataaccctga attagacggt ttcttatagc tttttctatc tggcaggcag
     2461 cctgtcgata
//
LOCUS       DAONFI010000336         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_55275_length_2466_cov_5.144753, whole genome shotgun sequence.
ACCESSION   DAONFI010000336 DAONFI010000000
VERSION     DAONFI010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55275_length_2466_cov_5.144753"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLK08_09475"
     CDS             complement(<1..616)
                     /locus_tag="PLK08_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53770.1"
                     /translation="MKLRRSILTNLHPAQMQRHVKPRLAISPQVFRGFTLIELLVVIA
                     IISLLVSILMPSLNKAKDLARQVTCSANIKNINLGFNLYVNDWEGYFPLVRETVAPTG
                     TWADKLYNDYIKDVNVFMCPSVPERTFTPNVSQGARLMAYGMEWWLGGGANIFASSWK
                     GRRLIDIANPSITVLLGENNNSSTCHGYGLHNYLWPDNIIYQWGW"
     gene            complement(638..>2466)
                     /locus_tag="PLK08_09480"
     CDS             complement(638..>2466)
                     /locus_tag="PLK08_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HPS53771.1"
                     /translation="MSNPWGQSLEVAQRLNNWTAFNEYFVDSPDITPEFYYRFLVSLL
                     EQTRYMYAIENMGFLAGNWPIIECGGLAQAAIMFPEFKEAPKWWAKVKQLTKMQMRVT
                     VFPDGVQVERSPGYHTWCYGQFNNLRLLGAMNNIELPEGFDKTVKGMYEYRHYKIAYP
                     DRSGEPAMADGSGVSWWVKSGKAAKGSLNSQSLKNLWNLGLVEYETLKKFEKSTPPYT
                     STELTSAGFYVMRSGWGDKERFLLFDCATPSTAGGHWHSSALDVDIYAFGRPLIVDPG
                     ASGYSSVSYLDYFRRARAHNIIEFCSEENRRDPKLIRWLRSKDFCLAEGAVEGPNEAS
                     MTRRVLFVGGDYWIIDDLANVPSRHPVITREYWHLNSQSVVAGGKQVQLAFEGSGFKK
                     QCWAGEDGQDLSFYTNDPNTGNILVVPDGSLRYASLMLSADTPCLGYVASYEQLPVPP
                     VKIALRYTTLLYPFEGTKRPDVSFKDGVVKIGKNREGLYMRKGEKTDGDCAFVGKEDG
                     AINRILLVAGSRISGTVQLDVPADFMIATRQGESVEIQLMEGSSKKVNRIELPGLQGV
                     SKVMVNGKTCKISETDGKLVIAGPFEKISPNPDNLDLFWIVK"
BASE COUNT          656 a          668 c          508 g          634 t
ORIGIN      
        1 gccaacccca ttgataaata atattatccg gccacaaata attatgcaaa ccataaccgt
       61 gacaagttga actattgtta ttttcaccca ataaaacagt tatcgacgga ttagctatat
      121 cgatgagccg acgccctttc caactcgatg caaatatgtt agcacctccg cccagccacc
      181 attccatacc gtaagccatc agcctcgctc cctggcttac attcggcgta aaagtccttt
      241 ccggaacact gggacacata aaaacattta cgtcttttat gtaatcatta tacaacttgt
      301 ctgcccacgt accggtaggt gctaccgttt ctcgtaccaa cgggaaatag ccttcccaat
      361 cattaacata caggttaaac cctagattta tgtttttgat attcgcagag caggtaacct
      421 ggcgggccag gtctttggct ttattcaagc tgggcatcag aatcgatacc aatagcgata
      481 taatcgctat cactaccaac aattctataa gagtgaaccc acgaaagact tgcggagata
      541 tggcaagtcg gggcttaaca tggcgttgca tttgcgccgg gtgcagattc gtcaatatag
      601 aacgtcgcag tttcataatc tgcctccatc tattatttta cttcacaatc cagaataaat
      661 caagattgtc ggggttggga gagattttct cgaaaggccc ggcaattacc aatttgccgt
      721 cagtttcgga tattttacaa gttttgccgt tcaccattac tttactgacg ccttgcaagc
      781 caggcagctc tatgcgattg acttttttgg aagaaccttc cattaattgt atttcaaccg
      841 actcaccttg acgcgttgct atcatgaagt cagcaggaac gtctagctga acagtacccg
      901 atatacggct gccggcaacc agaagtatac gattaatcgc tccatcttct ttaccgacga
      961 acgcacagtc gccgtcggtc ttctcgcctt tacgcatata gagaccttcg cgatttttac
     1021 cgattttgac cacaccgtcc ttgaaggaaa catccggccg ctttgtaccc tcaaacggat
     1081 acagcagcgt cgtatagcga agagctatct ttaccggagg aacaggaagc tgttcataac
     1141 tggcgacata acccagacaa ggagtgtctg cactcaacat cagggacgca tatctcagtg
     1201 aaccatcggg aacaaccaat atgttgcctg tgttaggatc attcgtatag aacgacaaat
     1261 cctggccgtc ctcgccggcc cagcattgtt ttttgaatcc gctaccttca aatgccagct
     1321 gaacctgctt gccgccggca accacggatt gggaattcaa atgccaatat tcacgagtta
     1381 taaccggatg ccgcgaagga acattagcca aatcgtcgat aatccaataa tcgccgccga
     1441 caaacaatac ccgccgcgtc atggatgcct cattcgggcc ttcgaccgca ccttcagcca
     1501 ggcagaaatc tttcgacctc agccagcgta tgagcttcgg gtctcgtcgg ttttcttcgc
     1561 tacagaactc gattatatta tgtgccctgg cgcgacggaa ataatccaga taactgacgg
     1621 aagaatagcc cgatgcacct gggtctacaa tcagcggccg accaaacgca taaatatcga
     1681 catccagcgc actactgtgc caatggccac cggcggtaga cggtgttgca caatcgaaaa
     1741 gaagaaatct ttccttatcg ccccaacctg accgcatcac gtaaaaacca gccgacgtca
     1801 attcggtcga agtatacggc ggagtagatt tctcaaattt tttcagtgtt tcatattcca
     1861 caaggccaag attccacagg tttttcagac tttgactatt gaggctgcct ttggcggctt
     1921 tcccactttt tacccaccat gaaacgccac tgccgtcagc catggccggc tcaccactcc
     1981 tgtcagggta cgcgatttta taatgccgat attcgtacat gcccttgaca gttttgtcaa
     2041 aaccttccgg cagttcgata ttgttcattg cccctaacag ccgcaaatta ttaaactgtc
     2101 catagcacca cgtatgatag cctggcgacc tctcaacctg tacgccgtcg gggaaaaccg
     2161 tcacgcgcat ctgcattttc gtcagttgtt tgacctttgc ccaccatttt ggggcttcct
     2221 tgaattcagg gaacattatc gccgcctgag ccaggccgcc gcattctatt atcggccaat
     2281 tgccggcaag aaagcccatg ttttcgatag catacatgta acgcgtttgt tccagcaggc
     2341 tgacaagaaa acgatagtaa aattcgggag ttatatcagg cgaatctacg aagtattcat
     2401 taaatgccgt ccaattattc agccgctgtg ccacctccaa cgactgcccc cacggattgg
     2461 acatca
//
LOCUS       DAONFI010000337         2462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_55409_length_2462_cov_3.802243, whole genome shotgun sequence.
ACCESSION   DAONFI010000337 DAONFI010000000
VERSION     DAONFI010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55409_length_2462_cov_3.802243"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLK08_09485"
     CDS             <1..416
                     /locus_tag="PLK08_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HPS53772.1"
                     /translation="RADVIHSDRLQRERKQVMDDFRSGKTPILVASDIAARGIDVDDI
                     SHVINYELPRDPEVYIHRIGRTGRAGETGVAISFCDIEERLMLDEIQKLIGHNIPVIE
                     NHPYPSHVPLKREEEPKPLATGIHSNRRPGRRRRF"
     gene            complement(468..2000)
                     /locus_tag="PLK08_09490"
     CDS             complement(468..2000)
                     /locus_tag="PLK08_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPS53773.1"
                     /translation="MTLQSIEESNEVEYGTIASVQAVPVRVDDVSSDNNSLAANMTGY
                     SKSSSGGKFWTVGTLRYTTRGLIVLFGWLLIGDFVWSMRDRAIGAVSQVMLKKYGASD
                     FMVGLLLVSLPQAIALFLGPTVSCWSDRCRTRWGRRIPFLIIMAPIGALAVIGFAFSP
                     MIGKWFHNLIGASPNTADIFAIATFGVFWTIFDISSITANTVFGGLVNDVVPHNIIGR
                     FYGLFRAASLLAGIIFFYVLMGKAEDYYMAIYLGLGVLYGVGFTSMCFNVKEGGYPAP
                     PSMSGSKGVHPLKSVKSYFYECFSSSYYVWYYMMQAMSALAMTATFIFSVYAARSFGL
                     SLGDYGLCLVFTYIGSLVLSYPLGIMVDKFHPLRMTLLALFLHVLITFCGFFFIVSPT
                     AFAIIFTIQGICAGTYGTCASGLPTRLLPKSRFTQMVSAGGMVASILTIVFAAIFGKF
                     LDIVDHQYQYTFGVGCLFTVLALIAGIYLYRGFLSLGGLKNYVPPEPASIADGENINK
                     LE"
     gene            complement(2074..>2462)
                     /locus_tag="PLK08_09495"
     CDS             complement(2074..>2462)
                     /locus_tag="PLK08_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53774.1"
                     /translation="ASTLDKTAEAAKNPPVRIVRKDDKIHAIEKSDSSCATGVFFDAG
                     ECKIGGLDKPGYLAWEKKGSNVNVAVFMPEAGEVKVTLPFAIDTKKLPSSIKVKTVND
                     KFSTVTITVASRVQSTICLPLISANK"
BASE COUNT          756 a          591 c          499 g          616 t
ORIGIN      
        1 tgcgtgcgga tgtgattcat tccgataggc tgcagcgtga acgcaagcag gttatggatg
       61 actttcgcag cggaaaaacg ccgattctcg tcgccagcga tattgccgca aggggcattg
      121 acgtcgatga tatttcgcac gtcataaact atgaattgcc tcgcgacccg gaagtgtata
      181 ttcatcgtat aggccgtacc ggcagggcgg gtgaaaccgg agtggcaata tcattctgcg
      241 atatagaaga acggctgatg cttgatgaaa tccagaaact tataggccat aatattcctg
      301 taatagagaa tcatccctat ccatctcatg tgccgctgaa aagggaagaa gaacccaagc
      361 cgttggctac cggcattcac agtaaccgtc ggcccggccg tcgacgcaga ttctagtact
      421 ctgtccaggc taggttgttt ggtataatct tttcagtttg attctggcta ttcgagttta
      481 ttgatatttt ctccgtcggc tatgctggcc ggttccggtg gaacatagtt ctttaagccg
      541 cccaaagaga gaaaaccacg ataaagatat ataccagcta tcaaggccaa tacggtaaac
      601 aaacaaccca cgccaaatgt atattgatat tgatgatcta ctatatcaag aaatttaccg
      661 aaaatcgcag caaaaactat cgtcaatatg ctggctacca taccaccggc ggaaaccatc
      721 tgcgtaaacc gtgacttcgg aagcaatctt gtgggtagtc ccgacgcaca tgtaccatat
      781 gtgccggcac atatgccctg aatcgtaaag ataattgcaa aagcggtcgg actcacaatg
      841 aaaaagaatc cacaaaatgt tatcaaaacg tgtaaaaata aagctaacag agtcatacgt
      901 aacggatgaa atttatcaac cattattcca agcgggtacg acaacactag cgaaccgatg
      961 tatgtgaaca ctaggcaaag gccataatca cctaatgata aaccaaaact tcgtgcggca
     1021 tatacactga aaatgaaagt cgccgtcatt gccaatgctg acatcgcctg catcatataa
     1081 taccatacgt aatatgacga tgaaaaacat tcgtaaaaat acgacttaac ggatttgagc
     1141 ggatgaacgc cctttgagcc ggacatcgac ggcggtgcag gatatccacc ctccttgaca
     1201 ttgaaacaca tggacgtaaa acctacaccg taaagaacgc ccaggcctaa atatatcgcc
     1261 atgtaataat cttcagcctt ccccatcaat acatagaaaa agattatgcc ggcaagcaga
     1321 ctggcggcac gaaataatcc gtaaaaccga cctattatat tgtgtggcac gacgtcattt
     1381 accagcccgc cgaataccgt atttgccgtt attgatgaaa tatcaaaaat cgtccagaac
     1441 accccaaatg tagctattgc gaatatatct gctgtatttg gactggcacc aatcagattg
     1501 tggaaccatt ttccaatcat aggactgaat gcaaaaccga taacggctaa cgctcctatg
     1561 ggagccataa tgatgagaaa cggaatacgt ctgccccagc gtgttcggca tcggtcactc
     1621 cagcaactta cagtcggacc gagaaataat gcgatagcct gcggcaagga aacgagcaac
     1681 aagccaacca taaaatcgga tgccccatac tttttgagca ttacctgaga taccgcacct
     1741 attgcacgat cgcgcatcga ccacacaaaa tcgcctatca gcagccatcc aaacagaact
     1801 atcaatcccc gagtcgtata acgaagagtc ccgaccgtcc aaaatttacc gccggaagag
     1861 ctttttgaat aacccgtcat attggcagcc agagaattat tgtcagatga cacatcatct
     1921 actcttacag gtacagcctg cacactcgca attgtgccat attcaacttc attactttcc
     1981 tcaattgatt gcagcgtcat ttgtattcaa tcctgacaat tccaaggtat tgacgtaaaa
     2041 tagttcgatg atattactat ctgcatttaa tactcattta ttagcggaaa ttaacggtag
     2101 acatatggta ctttggactc ttgaggcaac agttatggtt acagttgaaa atttatcatt
     2161 cactgtttta actttgatgg aagatggcag tttttttgtg tcgattgcaa aaggcaatgt
     2221 aactttaact tcaccggctt ctggcataaa aacagcaacg tttacatttg agcctttttt
     2281 ctcccaagcc aagtaaccag gcttatccaa cccgccaatc ttacattcgc cggcatcaaa
     2341 aaatacgcct gttgcacaac ttgaatccga cttctctatg gcatgaattt tatcatcttt
     2401 tcgaacgatg cgaacaggcg gattcttagc tgcctccgcc gtcttatcca atgtcgacgc
     2461 tg
//
LOCUS       DAONFI010000338         2437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_56322_length_2437_cov_3.834593, whole genome shotgun sequence.
ACCESSION   DAONFI010000338 DAONFI010000000
VERSION     DAONFI010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56322_length_2437_cov_3.834593"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1390)
                     /locus_tag="PLK08_09500"
     CDS             complement(125..1390)
                     /locus_tag="PLK08_09500"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPS53775.1"
                     /translation="MQLQEAKEFISDIARQSLKASATLAISSGAKRNEALHACANALR
                     DARADLQSENRKDLAQAKELGISPAMLDRLTLTDARVEGMAIAMEEIAAQTDPVGEAI
                     AAYNRPNGLRIEKRRVPIGVIGIIFESRPNVTADAAGLCIKSGNACILRGGREAIRSN
                     LAIAAAIRKGLAAAGLPEACVTVLDRTDHELVTAMATAEGLIDLIIPRGGENLIRAVT
                     QAATIPVIKHYNGNCHVYVHKSADPEMAKKIAMNAKTQRTGVCNAAESFLIDAAVADE
                     FLPTIGRALIDAGVEIRGCRKTCKLLPAAKPATEDDWAKEYLDMIIAIKIVDDIDAAI
                     EHINRYSSKHTDAIVTRDLAAAEKFATAIDSASVMINASTRFSDGGEYGLGAEIGIST
                     DKLHARGPMGAADLTTYKWIVTGNGHIRT"
     gene            1500..2141
                     /locus_tag="PLK08_09505"
     CDS             1500..2141
                     /locus_tag="PLK08_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPS53776.1"
                     /translation="MGVEIKWLGHSCFRMADGSLAVYTDPYNLAAAVNDGDVICISHA
                     HYDHCSPDDIARIAKPDATIVCTEDVVSQVGNFLKNIVVISPGRTVNVGSLKIEGVAA
                     YNIGKKFHPQDSGWCGYVLNIGGRRIYYAGDSDRIPEMKNLKNIDLAILPVGGTYTMT
                     PQEAAAAVDDIGCVEAIPCHWGSVIGSEADAKNFISLIKCEGILLKFGQSTKI"
     gene            complement(2314..>2437)
                     /gene="pheS"
                     /locus_tag="PLK08_09510"
     CDS             complement(2314..>2437)
                     /gene="pheS"
                     /locus_tag="PLK08_09510"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375398.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPS53777.1"
                     /translation="PEKYTGWAFGFGIERLVMRKHAITDIRMLFESDIRFLEQF"
BASE COUNT          556 a          602 c          648 g          631 t
ORIGIN      
        1 tggtgacagc cgccaatatg cccgtcaaga caaccacagt caacaactcc accagagtga
       61 acgcagtata actgtctgtc agatggcgac acttcatatg cttttcctga aaaccgtaga
      121 ttcgtcaggt tcttatatgg ccgttgccgg tgactatcca cttgtacgtg gtcaggtcag
      181 ccgctcccat cggcccgcgg gcatggagtt tgtcggtact tatgccgatt tctgcgccaa
      241 ggccatattc tccgccgtcg ctgaaacggg tggacgcatt tatcattacg ctggcggaat
      301 caatcgcagt ggcgaatttt tccgcagcgg caaggtcgcg ggtgacgatg gcgtcggtat
      361 gtttcgaact gtagcgattt atatgctcta tggcggcgtc gatgtcgtcg acgattttta
      421 tggcgattat catgtccagg tattctttgg cccagtcatc ttccgtcgcg ggttttgccg
      481 ccggcagcag tttgcaggtc ttccggcagc cgcgtatttc aacgccggca tcgatgagtg
      541 ccctgcctat cgtcggcaga aattcgtccg ctaccgccgc gtctatcaaa aaactttcag
      601 ccgcattgca tacgcccgta cgctgggtct tggcgttcat ggcgattttt tttgccattt
      661 cagggtcggc ggatttgtgg acgtaaacgt ggcagttgcc gttataatgt tttatcaccg
      721 gtatggtggc tgcctgggtg acggcacgta tcagattttc gccgccgcgg ggaattatca
      781 aatctatgag tccttcggcg gtagccatgg cggtgacgag ttcatggtcg gttctgtcga
      841 ggactgttac gcacgcttcg ggcaggccgg ccgcggctaa gcctttgcgg atagccgcag
      901 cgatagccag attggaacgg atagcctccc tgccgccgcg aagaatacag gcattgccgc
      961 tttttatgca aaggccggcc gcgtcggctg tgacgttggg ccgcgattcg aaaattattc
     1021 caatcacgcc gattggcacg cgacgttttt ctatgcgaag gccattgggc cggttgtatg
     1081 ctgcgatggc ttcgccgact gggtcggtct gggctgctat ctcttccata gcgatggcca
     1141 tgccttcgac gcgggcgtct gtgagcgtca acctgtcaag catagccggt gaaattccga
     1201 gttctttcgc ctgagcaagg tcttttctgt tttcgctttg caggtcggcg cgggcgtccc
     1261 gcaaggcgtt cgcacatgca tgcaaagcct cattacgttt cgcgccgctg ctgatggcta
     1321 atgtcgcact tgccttgagc gactgccttg cgatatcgct tatgaattct ttagcttcct
     1381 ggagttgcac ttgaactctc cggtaatcat ttgaaaaaga acctttttaa cactaaagtg
     1441 tatatgataa taacaagcgt cgaattttcc aagcactctt tgtcataagg agttgtaaaa
     1501 tgggtgtaga aataaagtgg ctggggcatt cgtgtttccg catggcagac ggctcactgg
     1561 cggtttatac cgacccttac aacctcgccg ccgctgtgaa tgacggcgac gtcatctgca
     1621 tatcgcacgc acactacgac cattgttcgc cggacgatat cgcccgcata gccaagccgg
     1681 acgcgactat cgtctgcact gaagacgtcg tcagtcaggt cggaaatttt ttaaaaaata
     1741 tcgtggttat ctctcccggc aggacggtca atgtcggcag cctgaaaata gagggtgtag
     1801 cggcgtataa catcggcaag aaatttcatc cccaagacag cggctggtgc ggatatgtgc
     1861 tgaatatcgg cggcagaagg atttattacg ccggcgattc ggacaggata ccggagatga
     1921 aaaatttgaa aaatatcgac cttgccatac tgccggtggg cggcacttat accatgacgc
     1981 cgcaggaggc tgccgccgct gttgacgaca tcggctgcgt agaagccatc ccgtgccact
     2041 ggggcagcgt tatcggcagt gaggcggacg caaaaaattt catcagcctt ataaagtgcg
     2101 agggcatttt actgaaattc ggacaatcga cgaaaatata aaaaatcgct tcgaaacggc
     2161 aactgactat ataactaata gatgttttaa aattgtcaaa ataaactgtg aattttggat
     2221 aaacattttt aaaaattgtc taaataagct gcaaatttcg gataggcgtt ttaaaaattg
     2281 tctaaataaa cgataggttt cagataaaca tttttaaaat tgttcgagga agcggatgtc
     2341 agactcgaac agcattcgga tatctgtaat ggcatgcttc cgcatgacca gccgctcaat
     2401 gccgaatccg aacgcccagc ctgtgtattt ttccggg
//
LOCUS       DAONFI010000339         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_56926_length_2420_cov_3.544609, whole genome shotgun sequence.
ACCESSION   DAONFI010000339 DAONFI010000000
VERSION     DAONFI010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56926_length_2420_cov_3.544609"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="PLK08_09515"
     CDS             complement(<1..2359)
                     /locus_tag="PLK08_09515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPS53778.1"
                     /translation="MSQNGKKIVIVGGVAGGASAATRARRMDENASIVMFERSGYVSF
                     ANCGLPYHIGETISKREKLLVTTAKALWERFRIDARVRHEVTAINRSRKTVTVKKLDD
                     GSIFEQPYDALLLSPGGKPTLPDIPGIHLPNVHALRVIEDMDAIKSAVDRSGTKSAVV
                     VGGGFIGLEVAEELVRRNLAVTMLQSNRQVFSQCDYEMASPLIQQLLSHGINLRVKTT
                     AKAIAAIDGGRLAVTTSHDDTIEADLVVIGIGVRPEAHLAGDAGLELGPTGGIKVDKS
                     MRTTDPNIWAVGDAVEVTNFVTGKPVLIPLAGPANRQGRLAADAMLGRTVEYNASQGT
                     AICKVFDATVAVTGISERIAKAQNIPCQAVFVHPQNHAGYYPGAVPMTVKLVFSPQDG
                     KILGAQVVGTEGVDKRIDVFATALRAGLTVFDLEELELAYAPPYGSAKDPVNMAGFAA
                     ANVMRGDTAICHSQDIIDRDRQKVFLLDVRTPEEFALGTIDDAKNIPLDSLRERLNEL
                     PKDKELIVFCQAGLRGYIASRILLQNGFTECKNLSGGYKIWKMYNQPAPNSTPITACG
                     KEMEAIKMNEQAAPAGGASEIVRDIDACGLQCPGPIMQLKAAIDEVKTGQSVRIRTTD
                     AGFIADVPAWCGSTGNTLINLGDAEKGVYSAVITKGHAAATQETKSAATEPAGRDKTI
                     VVFSNDFDKVMAAFIIANGAAAMGSKVTMFFTFWGLNVLRRKEKIGVRKNLVEKMFGW
                     MMPRGSDKLPLSKMNFAGLGKRTMLGIMRKKNVATLDELIGSARLA"
BASE COUNT          416 a          670 c          691 g          643 t
ORIGIN      
        1 cggccagcct ggcgctgccg attagttcat ccagcgttgc gacatttttt ttacgcatta
       61 tgccgagcat tgtgcgtttg ccgaggccgg cgaaattcat cttcgagagc ggcagtttgt
      121 ccgagccgcg cggcatcatc catccgaaca ttttttcgac taaatttttc cgtacgccga
      181 ttttttcttt tcttcgcaat acgttcagcc cccagaatgt gaagaacatc gtaactttcg
      241 aacccatagc ggctgcgccg tttgctatga tgaatgccgc catcaccttg tcgaagtcgt
      301 tgctgaatac gacgatggtt ttatccctgc ctgccggctc tgtagcggcg gattttgttt
      361 cctgtgtcgc cgccgcatgg ccttttgtga tgacagcgct atagacgcct ttttcggcat
      421 caccgaggtt tatcagcgtg ttgccggtgc tgccgcacca tgccggcacg tcggcgatga
      481 atcccgcgtc ggtagtgcgt atgcggactg actggccggt tttgacttca tcgattgcag
      541 ccttgagctg cattatcggc cctggacatt gcagcccgca ggcgtcgatg tccctgacga
      601 tttcagatgc tccgccagcc ggagcggcct gttcattcat cttgatagcc tccatttcct
      661 ttccgcacgc ggttattggc gtggagttcg gggccggctg attatacatc ttccagattt
      721 tgtaaccgcc ggagagattt ttacattctg taaaaccgtt ctgcagaagt attctgctgg
      781 cgatgtatcc tcgaaggcca gcctggcaga agacaatcag ttctttatct ttcggcagtt
      841 cgttcagccg ttcccgcagg ctgtccaggg ggatattttt cgcgtcgtcg attgtgccca
      901 gggcgaattc ttccggcgtt ctgacgtcga gaagaaatac tttctggcgg tcgcggtcga
      961 ttatatcctg gctgtggcag atggcggtat cgccgcgcat cacgtttgcc gcggcaaaac
     1021 cggccatgtt tacagggtct ttggctgagc cgtacggcgg cgcgtaagcc aactcaagtt
     1081 cttctaggtc gaataccgtc aagccggctc tcaatgccgt cgcaaaaacg tctatgcgct
     1141 tgtctacgcc ttccgtgccg accacctggg cgccgagaat ttttccgtcc tgcggcgaaa
     1201 ataccagttt gacggtcatc ggcactgcgc cagggtagta tccggcgtgg ttctgcggat
     1261 gcacgaagac cgcctggcag gggatatttt gagcttttgc gatgcgttcc gaaatgccgg
     1321 taacggcgac ggtggcgtcg aagaccttgc agatggctgt gccctgagag gcgttatatt
     1381 cgaccgttct gccgagcatg gcgtctgcgg cgagtcggcc ctgccggttt gccggcccag
     1441 ccagcggtat gagcaccggc ttgccggtga cgaagttcgt aacttccacc gcgtcgccga
     1501 cggcccatat gttcgggtcg gtggtgcgca tggatttgtc gacttttatt ccgccggtcg
     1561 ggccgagttc gaggccggcg tcgccggcca gatgtgcctc cggccgtacg cctatgccga
     1621 ttaccaccag gtcagcctcg attgtatcgt catgcgatgt cgttaccgcc agccgtccgc
     1681 cgtcaatcgc ggcgatggcc tttgccgttg tttttacgcg aagatttatg ccgtggctga
     1741 gaagctgttg aatcagcggg ctggccattt cgtaatcgca ctgcgagaat acctggcgat
     1801 tggattgcag catagtgacg gccaggttgc ggcgtacgag ttcttccgca acttcaaggc
     1861 cgatgaagcc gccgcccaca acgacggctg attttgtgcc gctgcggtcg acggcagatt
     1921 taattgcgtc catgtcttcg attacccgca gcgcgtgaac gttgggcaga tgtatgcctg
     1981 gaatgtccgg cagagtcggt ttgccgcccg gcgagagcag cagggcgtcg tacggctgtt
     2041 caaatatgct gccgtcgtcg agttttttga ctgtcacggt tttgcggctg cggttgatag
     2101 ccgtcacttc atgccgcacg cgggcgtcga tgcgaaacct ctcccacaac gccttcgctg
     2161 tcgttacgag gagtttttca cgtttggaga ttgtttcgcc gatgtgatac ggcaggccgc
     2221 agttggcaaa tgagacgtac ccgctgcgtt cgaacatgac gatgctggcg ttttcatcca
     2281 tgcgtcttgc tcgcgtggcg gctgatgctc cgcctgcgac gccgccgact atgacgattt
     2341 ttttaccgtt ttgcgacatt tttttaaaaa ctcctatttt ttctttgacg cagtgttcca
     2401 ttcgggacag ttttgttgca
//
LOCUS       DAONFI010000340         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_57368_length_2407_cov_4.869898, whole genome shotgun sequence.
ACCESSION   DAONFI010000340 DAONFI010000000
VERSION     DAONFI010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57368_length_2407_cov_4.869898"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1803)
                     /locus_tag="PLK08_09520"
     CDS             complement(130..1803)
                     /locus_tag="PLK08_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPS53779.1"
                     /translation="MSNFGNMQKYHYSCIAELATELCSGLSRLRQGFIDRSEEMLDVI
                     EPEKSYPLDFVVFRITNYRQTDAAAKPMLDGKKLRHDLINLILEVSESFDLSTGDYEE
                     QVFDTAGLAERFNVSTKTIQRWRNRGLTARKMLFPDGKKRIGFLESSADSFSSTFEDI
                     LERSSRFSQMSESEKNEILRRARRMVSFTDTNLMEVARRIARKSGRAVETIRYTIRNH
                     DNLSPNEAIFPKLLNILSKEEKQRIYQKFLTGTSARSLAQEYGRTRGSIHRIVNEVRA
                     LQLLERSITYIYSELFEDQGMEKDMLRMATMEVSEKDLVRSRPPEGLPQYLKDLYQVP
                     LLSAPQEKALFYLYNFYKYRADHLRKRIDPTHIQAHQVKRVESLLLEANVINNRIVKS
                     NLRLVVSIAKKHVSGSIDLFELVSEGNIALMRAVEKFDFSRGFRFSTYASWAIMRNFA
                     RSVPKEQNQLARFSTGHEEILDIAAALGNYDPGSESMGEVRETLESMLSHLSPRERTI
                     LTEHYGLAESAQASTFEQIGKKLNISKERARQIELQALEKLRDIAGPHQ"
     gene            complement(1860..>2407)
                     /locus_tag="PLK08_09525"
     CDS             complement(1860..>2407)
                     /locus_tag="PLK08_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53780.1"
                     /translation="QKEFCANVDAGQNGFEKSADSLKRALQNASDDFRNSMQLKQAEL
                     ALAAASIELDMIAYKKQVENLQNTLTAGNIGIAVDESVKKIAADALAEAAVKNCQDSI
                     STLERVLRDTPSTGKWRMHSALASANITLSRSFRAAGNASLADNALKNAQTQLAEARN
                     KSKWLAKASLDAIETQIRQAK"
BASE COUNT          479 a          580 c          646 g          702 t
ORIGIN      
        1 agcctttaat atcttatttt ttgcggccgc agggtaattg agaacggcct ctagagcata
       61 tcaagtttaa catgctctaa atatcatttt ggacgatatg gccgttttac ggcagatgat
      121 tggaccgatt tattgatgtg ggccggcgat gtcccgaagt ttctcaagag cctgcagttc
      181 tatctggcgt gcgcgttcct tgctgatgtt gagttttttg cctatctgtt cgaacgtgct
      241 ggcctgagcc gattcggcaa ggccgtaatg ttccgtcaga attgtccgct cccgcggcga
      301 aagatgacta agcatggatt ccagcgtttc acgtacttcg cccatgcttt cgctgcctgg
      361 gtcgtaattt cccagggcgg ctgctatgtc cagaatctct tcatggccgg tggagaacct
      421 cgcgagctga ttctgttcct tcggaacgga acgggcgaag ttgcgcatga tagcccatga
      481 tgcataggtg gaaaatctga acccgcgcga gaagtcgaac ttttccacgg cgcgcatgag
      541 cgctatgttg ccttcgctta cgagttcaaa gagatctatc gagccggaaa cgtgcttttt
      601 tgcgatagac accaccagcc gcagattcga cttgacgatg cgattattga tgacgttagc
      661 ctccagcagc agtgattcga cgcgtttgac ctgatgcgcc tgaatgtgcg ttgggtcaat
      721 tcgtttgcga aggtggtcgg ctcgatattt gtaaaaattg taaaggtaaa aaagggcttt
      781 ttcctgcggc gccgacagca gcggcacttg gtagaggtct ttgagatatt gcggcaggcc
      841 ttcgggcgga cgggaacgga cgaggtcttt ttcgctgact tccatcgtcg ccatcctgag
      901 catgtctttt tccatgccct ggtcttcgaa gagttcgctg taaatatagg ttatggaacg
      961 ttccagcagt tgcagtgcgc gaacttcatt gactatcctg tgaatgctgc cgcgggttct
     1021 gccgtattcc tgtgccagcg acctggcaga cgtgccggtc aggaatttct gataaatccg
     1081 ctgcttttcc tcctttgaga ggatgttgag cagtttgggg aaaatcgctt cattcggcga
     1141 cagattgtcg tggtttcgta tcgtgtaacg gatggtttca acggcacggc cgcttttgcg
     1201 ggctattcga cgggccacct ccatcagatt ggtgtccgtg aatgaaacca ttcttctggc
     1261 acggcggagg atttcattct tttcactttc ggacatctgt gaaaaacggc tggaacgttc
     1321 gagtatgtct tcaaacgtcg aagagaacga gtcggctgag ctttcgagaa agcctatccg
     1381 ttttttgccg tccggaaaga gcatttttct ggcggtcagc ccgcggtttc tccatcgctg
     1441 tattgtcttt gtcgatacgt tgaaccgttc agccagcccg gcggtatcga aaacctgctc
     1501 ttcataatcg ccggtcgaca aatcgaaact ctcggatact tcgagtatga ggtttatgag
     1561 atcatgcctg agttttttgc cgtcgagcat cggtttggct gcggcgtcgg tctggcgata
     1621 gttggttatc cggaagacca cgaaatccag cggataactt ttttcaggct cgattacgtc
     1681 cagcatctct tcggaacggt cgatgaagcc ctgacgcagg cgtgacagcc ctgagcacaa
     1741 ttctgtggcc aattctgcaa tgcaactata atgatatttc tgcatatttc cgaaattact
     1801 catatatttg ccggcgcgtc aggcaattgc cgatttgcag aacggtccgg tcaaaaaaat
     1861 cattttgcct gtcttatctg cgtttcgatt gcgtccagcg acgccttggc cagccatttg
     1921 gatttatttc gtgcttcggc taattgtgtt tgggcgtttt tcagggcgtt atctgcgagc
     1981 gacgcattgc cggctgcgcg aaatgagcgg cttagcgtta tattcgcgct ggccaatgcg
     2041 ctgtgcattc tccatttgcc ggttgacggc gtatcgcgca gtacccgttc cagggtggat
     2101 atggaatcct ggcagttttt gacggcggct tcagccagtg cgtcggctgc gatttttttt
     2161 acgctttcat ctacggcgat gccgatattg cctgcggtga gagtgttttg aagattttca
     2221 acctgttttt tgtaggctat catgtctagt tctattgatg ccgccgccaa cgccagttcc
     2281 gcctgtttga gttgcatgga atttctgaaa tcatccgatg cgttctgcag agctcgttta
     2341 aggctgtccg ccgatttttc aaagccgttc tggcctgcgt cgacatttgc gcagaattcc
     2401 ttttgca
//
LOCUS       DAONFI010000341         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_57896_length_2393_cov_4.041916, whole genome shotgun sequence.
ACCESSION   DAONFI010000341 DAONFI010000000
VERSION     DAONFI010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57896_length_2393_cov_4.041916"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2050)
                     /locus_tag="PLK08_09530"
     CDS             complement(<1..2050)
                     /locus_tag="PLK08_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPS53781.1"
                     /translation="MNWMWTWPETVAATNDMFITVLKLMEEFPEFCFSQSQASVYEIT
                     REYNPALFSKIKSRIAEGRWEVTASEWVEGDKNLPSGESLVRHLLYTRRYMAENFGLK
                     PEDVSISWNPDTFGHACTIPMIYTRGGVSRYYMCRGGETPKPPIFWWQGADGSRLLVN
                     LEHTWYNDVIGPHNIKGLLSFCEKTGLNDWMLVYGVGDHGGGPTRRDLHMCIEMNSWP
                     IYPNFKFSTTRKYYEILEKHSQKFPVVNGELNYEFTGCYTSQSDIKKTNRLSECLLER
                     AEFAASLANVILGASYPNEVLRNAWQATLFGHFHDILPGSGIKTTREYHSGQFQNTAA
                     ATGMIITNSLRAIAGDIDTSFAGESEAIKDDVHFGAGMGLGGNVNIGGISTACHVSGV
                     KRGFVVFNPTAIRRSEVIKLTAWDAEHDSVHTKKFVVRTSDGNEIPAQRLYSSTLWGH
                     NYVELAVPVTVSAMGYSAFVLDTIGENEPVVENSQGYQRLQKEEVGLAKNFAFPRPLR
                     EYFAGDWGMENEFIRVAFDKTTGGITELVDKTTGVNLVAAGSQTAILEYFLEQPGSMS
                     AWLMRPARKSVCPIETISFQPAKFGPHLTAYEGVYKINDSTIKMTYSLSAGSPRVDIN
                     VEVDWLERGGPQIGTPALRMQFPFALENTMGRYEIPFGSIERKPQAGQEVPSQKFVDV
                     T"
BASE COUNT          634 a          622 c          477 g          660 t
ORIGIN      
        1 ctgtaacgtc aacaaatttc tgcgacggca cttcctggcc agcctgcggt ttgcgttcaa
       61 tcgaaccgaa cgggatttca tatcgaccca ttgtattttc aagtgcaaac ggaaattgca
      121 tcctcaatgc aggagtgccg atttgcggcc cgccgcgttc aagccagtcg acttccacat
      181 ttatatcaac tcttggagaa cccgcagata aagaataagt catttttatc gtcgagtcgt
      241 taattttata aactccttca tacgccgtca gatgtgggcc gaatttagca ggctggaaag
      301 atatcgtctc aatcggacaa acactcttcc gtgccggccg catcaaccaa gccgacatgc
      361 tgccaggttg ttccaaaaaa tactccaata tcgcagtctg tgaacctgcc gcaacaagat
      421 tgacgcctgt cgttttatcg acaagttcag taatgccgcc ggtagtttta tcgaaggcaa
      481 ctcggataaa ttcattctcc attccccaat caccggcaaa gtactcacgc aaaggccgcg
      541 gaaaggcaaa atttttagca aggcctactt cttctttctg taaccgctga tatccctgcg
      601 aattttcaac gacaggttcg ttttcgccaa ttgtatccag cacaaacgca gaatagccca
      661 tcgccgatac cgtcacaggc acagccagtt cgacatagtt atgtccccac aatgtactgc
      721 tgtaaagccg ctgagcagga atctcattcc catcagatgt tcgaacaaca aatttcttcg
      781 tatgtacgct atcatgttcc gcatcccacg ccgtcaattt tatgacctca cttcgtcgta
      841 ttgcagtcgg attgaacaca acaaaaccgc gtttgacgcc ggaaacgtga catgccgtag
      901 atattccgcc tatattgacg ttcccgccta atcccatgcc tgctccgaaa tgaacgtcat
      961 ctttgatagc ttctgactca ccggcaaacg atgtatcgat atcgccggca attgcacgca
     1021 gagaattggt tattatcata cctgtggcgg ctgcggtatt ctggaactgc ccgctgtgat
     1081 attcccgtgt tgttttaata ccagaccctg gcagaatatc gtgaaaatgt ccgaaaagcg
     1141 tagcctgcca tgcattccgc aacacctcgt tcggataaga cgccccaagt ataacgtttg
     1201 caagtgaagc ggcaaattcc gcacgttcaa gcaggcactc cgaaagtcgg ttcgtttttt
     1261 ttatgtcact ctgcgacgta tagcagccgg tgaattcata attaagttca ccattgacaa
     1321 ccggaaattt ctggctgtgt ttttcaagaa tttcgtaata ttttcgcgtc gtggagaatt
     1381 tgaaattcgg atatatcggc cagctattca tttcgatgca catgtgtaaa tcgcgtcggg
     1441 tcggcccgcc gccgtggtcg ccgacgccgt aaacaagcat ccaatcatta aggccggttt
     1501 tttcgcagaa agacaacagc cctttgatat tatgcgggcc gataacatcg ttataccatg
     1561 tgtgttccag attcaccaac aaacggctgc catctgctcc ctgccaccag aatatcggag
     1621 gttttggcgt ttccccgcca cgacacatat aatatctact cactccgccg cgagtatata
     1681 tcatgggtat cgtacatgca tggccaaatg tatcaggatt ccaggagatt gaaacatcct
     1741 caggcttaag cccgaaattc tcagccatat atcgccgtgt atagagaagg tgacgaacca
     1801 gactctcgcc gctaggcagg tttttgtcac cttcgaccca ctcactggcg gttacttccc
     1861 aacggccttc agctatgcga ctttttattt tcgaaaacag tgcgggatta tattcgcgtg
     1921 taatttcata aaccgacgcc tgactctggc tgaagcagaa ttctggaaat tcttccatca
     1981 gttttagaac cgtaataaac atgtcgtttg tggcggcgac agtttccggc catgtccaca
     2041 tccaattcat gtccatatgc gcatgagcca cgcaccaaca ggtatacttt tttgcagcct
     2101 tcccaatcgg cgaaagaatg gtttcaattt cgttccgcaa ctttaataaa accggtaaac
     2161 tttcagtcga cacggctaaa ttgagttttt cggcagccgt ttcaataatt ttatcccagg
     2221 aaggtttttt cagtaatttc gccaaacctc tggcaaactc aatctcagaa ttcagccgac
     2281 gaattacttt ttcactttca acaaattttt gcatcataaa ttatccaata ataaattttt
     2341 atactttaac aagaaattcc aatttctcag ttaatcatca actctacaat ttt
//
LOCUS       DAONFI010000342         2391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_57971_length_2391_cov_6.901969, whole genome shotgun sequence.
ACCESSION   DAONFI010000342 DAONFI010000000
VERSION     DAONFI010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57971_length_2391_cov_6.901969"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLK08_09535"
     CDS             <1..843
                     /locus_tag="PLK08_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPS53782.1"
                     /translation="KYDNVIVQMGNLLDGVAAHMADYPEYQAFRPADKPSATKEDICK
                     LLRDSGAEILMNYLPVGSQKATEFYAECCLETGISLINCIPVFIVSNPVWGKRFKDAG
                     IPCIGDDIKAQVGATITHRVLARLMTERGAKIDNTYQLNWAGNTDFLNMLERSRLKHK
                     KISKTEAVQSQLKNPLPADNVHIGPAEFVPWQKDNKVCELRIEARGFGNVPLNIEARL
                     SVEDSPNSAGETIDAIRVCKLARERKCVGPLLDVSAFTMKHPPVQYTDSEAKLKLDAF
                     IADK"
     gene            964..1425
                     /locus_tag="PLK08_09540"
     CDS             964..1425
                     /locus_tag="PLK08_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPS53783.1"
                     /translation="MSEKKYIVELTTKERSHLEEMIKAPRMMAHKRHHAQMLLKLDEG
                     PHGPAWADTKVAEAFDCTANSAKRLRRRLVEGGMAAALEHGNRGSTRAKSLDGVAEAH
                     IIAMACGDAPEGRNRWSVRLLADQAVASGIVDSCSKSSIHRILKKMNLSLT"
     gene            1440..2096
                     /locus_tag="PLK08_09545"
     CDS             1440..2096
                     /locus_tag="PLK08_09545"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HPS53784.1"
                     /translation="MPPDKNAEFVAAMEDVLDVYLRPENESKPLVCLDEASKQLISET
                     RSSLPCKSGNVRKVDAEYRRNGTASIFMMNVPLKGKRYVRVCEQRKREDFAEVVRELC
                     DKIYPDAEKIVLVMDNLNTHNTASLYQAFKPEEAKRLADKLEIHYTPKHGGWLNMAEI
                     ELSVLSRQCMKDYFETREQLADAIASWECSRNNMNAGINWRFTTADARIKLKSLYPTI
                     "
     gene            2201..2335
                     /locus_tag="PLK08_09550"
     CDS             2201..2335
                     /locus_tag="PLK08_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53785.1"
                     /translation="MKVLTREIWMEVPHRRQIISIHRQVEGLVAESGVREGLALINTK
                     "
BASE COUNT          671 a          557 c          630 g          533 t
ORIGIN      
        1 aaatacgata acgtcatcgt ccagatgggc aacctgctcg acggcgttgc tgcgcacatg
       61 gcggattatc ccgaatatca ggcatttcgg ccggcggata aaccctcggc tacaaaagaa
      121 gacatctgca agctgcttcg cgacagcggt gcggaaattc tgatgaacta tctgccggtc
      181 ggcagccaga aggctaccga gttttacgca gaatgctgcc tcgaaaccgg catcagcctc
      241 ataaactgca taccggtgtt cattgtttcc aaccctgtgt ggggcaaacg tttcaaagat
      301 gccggcattc cctgtattgg cgatgacatc aaggctcagg tcggtgcgac aattacacac
      361 cgcgtgcttg cgcggctgat gacggaacgc ggagcgaaaa tagacaacac atatcagctc
      421 aactgggcgg gcaatacgga ttttctgaac atgctcgaaa ggtcacgtct gaagcacaag
      481 aaaatctcga agaccgaggc tgttcaaagt cagctcaaaa atcctctgcc ggcagataac
      541 gtacacatcg gcccggctga gttcgttccg tggcagaaag acaataaagt ctgcgaactc
      601 agaatagagg cgcgcggctt tggaaacgtg ccgctgaata tagaagcccg cctgagcgtg
      661 gaagattcgc caaattcagc cggcgaaaca atcgacgcca tccgtgtctg caagttggct
      721 cgtgaaagaa aatgcgtcgg ccccctgctg gacgtaagcg cttttaccat gaaacatccg
      781 ccggtacaat atactgacag tgaagcgaag ctgaaacttg atgcattcat cgccgacaaa
      841 taaatttttg taacataact caaagtgacc gactagtgct ttgtcccgca aataatgtac
      901 agttcatatg gatcttttgc cgataaggct gatagaacct ttaccacggc aaggaggccg
      961 aaaatgtccg agaaaaagta cattgtagaa ttgacaacca aagaacgttc ccatctggaa
     1021 gagatgatca aggcccctcg aatgatggca cacaaacgtc atcacgccca gatgctgcta
     1081 aaacttgacg aaggtccgca cggcccggcg tgggctgaca cgaaagtggc tgaagccttc
     1141 gattgtacag ccaacagtgc caagcgtctt cgtcggcgtt tggtcgaagg cggcatggcg
     1201 gcagcgttgg agcatggcaa tcgcggcagc acacgagcca agtcgttaga tggagttgcc
     1261 gaagcccata ttattgcgat ggcgtgcggc gacgctcccg aaggccgtaa tcgttggtcg
     1321 gttcggctgc tggccgacca ggctgtagcg tcgggcatag ttgattcatg cagtaaatca
     1381 tctatccatc gcattttgaa aaaaatgaac ttaagcctca cctgaacaag tactggtgca
     1441 ttcctcctga taagaatgcc gagttcgtgg ctgcgatgga agacgttttg gacgtgtatt
     1501 tgcggcctga aaatgaaagc aagccgttgg tatgccttga cgaagcgtca aagcagctta
     1561 taagtgaaac gcggtcgagt ctgccgtgca aatcaggaaa cgtcagaaaa gttgacgcgg
     1621 aatatcgccg caacggaaca gccagcattt ttatgatgaa cgtaccattg aaaggcaaac
     1681 gttatgtgcg ggtttgtgag caacgcaagc gtgaagattt tgctgaggtg gttcgtgaat
     1741 tgtgtgacaa gatttatccc gatgcggaaa agattgtact cgtgatggac aatctcaata
     1801 cgcataacac cgccagcctg tatcaggcct tcaaacccga agaagccaaa cggctggccg
     1861 ataaacttga gattcattat acgcccaagc atggcggctg gctgaatatg gcggagatag
     1921 aattgagcgt gttgagccgt cagtgcatga aagattattt tgaaactcga gaacagcttg
     1981 ctgatgcgat tgcaagctgg gaatgttcaa gaaacaatat gaatgccggc atcaactggc
     2041 gattcacgac agccgatgcc agaataaaac tgaaaagctt atacccgaca atttaatcgg
     2101 gacaaagtac tagcctgttt tcaatgaatt ccttgatttt ccgttgtttt ttatgtttca
     2161 tatacatgta tttgtccttg caatgatggg aaggtacggc atgaaagttt tgacgcgaga
     2221 aatatggatg gaagtaccgc atcggcggca gataatttcg attcatcggc aggtcgaagg
     2281 ccttgtcgct gaaagcggag tccgcgaagg gttggcactc ataaacacga agtaaattta
     2341 gaacctatcc caaaaacatg ggcgtcgccg aaatcgagca attttgcggg c
//
LOCUS       DAONFI010000343         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_58021_length_2390_cov_4.163169, whole genome shotgun sequence.
ACCESSION   DAONFI010000343 DAONFI010000000
VERSION     DAONFI010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58021_length_2390_cov_4.163169"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="carA"
                     /locus_tag="PLK08_09555"
     CDS             complement(<1..841)
                     /gene="carA"
                     /locus_tag="PLK08_09555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPS53786.1"
                     /translation="MTQRAILAMEDGSVFEGTAFGAAGECSGEVVFNTSMTGYQEVLT
                     DPSYRGQIVAMTYPLIGNYGVNAADAESKGLWLSGFIVRELSRITSNFRSEKDLDTYL
                     RENGVMGIEGIDTREITKKLRTKGALRGVISTEETNHATLVKQAKNSPSLEGRNLVRE
                     VTAGEVFSWTQGLDGTSDVVEEKYNIVMMDAGAKYGIMRCLRSLGAKVTVVPFDTPAK
                     EIAAMNPDGIMLSNGPGDPEPVTEVIQTVRELMFNPPAGKVIPMFGICLGQQMIGLAL
                     GGKT"
     gene            complement(1272..>2390)
                     /locus_tag="PLK08_09560"
     CDS             complement(1272..>2390)
                     /locus_tag="PLK08_09560"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPS53787.1"
                     /translation="EETAVADALAAMNSKNRRRVEQAGDEISTTIFMADKMPDGVKAL
                     CPKCGGELTEPRMFNLMFETYVGAIRSDEAKAYLRPETAQGIFLNYKNIVDTMRVKMP
                     FGIAQIGKSFRNEVTPRNFIFRSREFEQMEMEWFCQPADARVWYDFWKEQRMNWWESL
                     GIEKSRLRYRDHDKEELAHYSQACVDVEYLYPFTSPDFGELEGIAHRGNFDLTQHQTH
                     SKVKMEYFDQDTNERYIPHVIEPASGLTRAVLVLLCEAFTPDANRPSQMFFKFKPKFA
                     PIKAAILPLVARDGMPEVAEKLYLALRDRHIVQLDVKQNIGKRYARQDEIGTPFCFTV
                     DGQTMQDNTVTVRDRDTLEQTRINLDQVEKFLADRIAE"
BASE COUNT          491 a          623 c          650 g          626 t
ORIGIN      
        1 atgttttgcc gcccagcgca aggcctatca tctgctggcc caggcatatg ccgaacatgg
       61 gaatcacttt gcccgccggc ggattgaaca tcagttcccg cacggtctga atgacttccg
      121 tgaccggctc agggtcgcca gggccgttgg agagcattat gccgtctgga ttcatggcgg
      181 cgatttcctt tgccggcgta tcgaacggca cgacggtgac cttggcgcca aggctgcgca
      241 ggcagcgcat gatgccgtat tttgcgccgg catccatcat gacgatgttg tatttttcct
      301 ccacgacgtc gcttgtgccg tcgaggccct gcgtccatga aaaaacctcg ccggcggtta
      361 cttcgcggac gaggtttctg ccctccaagg atggagagtt tttcgcctgt ttaaccagcg
      421 tcgcatgatt ggtttcttcc gtcgaaatta cgccgcgcaa tgcgcctttt gttctcagtt
      481 ttttggtgat ttcgcgcgtg tcaatgccct cgatgcccat cacgccgttt tctcgcagat
      541 aggtatcgag gtctttttca gagcggaaat tgctggtgat tctgctgagt tcgcggacga
      601 tgaatccgct cagccagagg cctttgcttt cggcatccgc ggcgttgacg ccgtaattgc
      661 cgatgagcgg ataagtcatg gcgacaatct ggccgcggta tgacgggtcg gtcaatacct
      721 cctgatagcc cgtcatagag gtgttgaaaa ccacctcgcc ggagcattct ccggcggccc
      781 cgaaggccgt tccttcgaac acgcttccat cttccatcgc cagaatagca cgctgggtca
      841 taaaaaaact cctgctgttt ttacctgttt tcatcaaaat acttgaaaaa cagcgttttc
      901 gccgtcaaaa ccggcgattt cttaacatgg gccaaggcct cgaaaaatat ccgcggcgca
      961 cgcgcacacc gcttactctc ggaatttaga ggatattagc aaaactcagt catttattca
     1021 atccatgtgt cgcaaattgc tcaatatttc cccaattttt tacttcgcgc gacaaaattg
     1081 taagttttaa tatgtccttc gtaattctac ggtttttaaa ccgtagatga aagtatccgc
     1141 cgtgaggcgg acaggcgaaa gcctgatgac tatgatgcta cgggttttaa cccgtagaga
     1201 ttcactattg cttatagcgg ttctcaaaaa attttagaga gccgctatgg tttatcgcat
     1261 aacataaaaa atcattcggc gattctgtcg gccaggaatt tttcgacctg gtcgagattg
     1321 atgcgggtct gttcaagcgt atcgcggtcg cgaacggtga cggtgttgtc ctgcatggtc
     1381 tggccgtcga cggtgaagca gaacggcgtg ccgatttcgt cctgccgcgc ataacgtttg
     1441 ccgatgttct gtttgacgtc gagttgtacg atatgtctgt cacgcagggc gaggtagagt
     1501 ttttcggcta cttcgggcat gccgtcgcgg gcgacgagcg gcaggatggc ggcttttatg
     1561 ggagcgaatt tcggcttgaa tttgaaaaac atctggctgg ggcggttggc gtccggcgta
     1621 aacgcttcgc aaagcagcac gagcacggca cgggtcaagc cgctagccgg ctcgataacg
     1681 tgcggaatgt accgctcgtt cgtgtcctgg tcgaaatatt ccatcttgac ctttgaatgc
     1741 gtctgatgct gagtcaggtc gaaattgccg cgatgcgcaa tgccctccag ttcgccgaaa
     1801 tccggcgatg tgaacgggta caaatattcc acgtccacgc aggcttgcga atagtgggcc
     1861 agctcttctt tgtcgtggtc acgatatcgc aggcgggatt tttctatgcc cagcgattcc
     1921 caccagttca tgcgctgttc tttccaaaaa tcgtaccaga cgcgggcgtc agccggctgg
     1981 cagaaccatt ccatttccat ctgctcgaac tcgcggctgc ggaagatgaa atttcggggc
     2041 gtgacttcat tgcgaaaact tttgccgatt tgcgcaatgc cgaacggcat tttaacgcgc
     2101 atggtgtcga ctatgttttt atagttcagg aaaatgccct gtgccgtttc cggacgcaga
     2161 taagctttgg cctcatcgct gcggattgca ccgacgtaag tctcgaacat gagattgaac
     2221 atgcgcggct cggtgagttc tccgccgcat ttggggcaaa gtgccttgac accgtcgggc
     2281 attttgtcgg ccatgaatat agtcgtggaa atttcatcgc cagcctgttc gactcggcgt
     2341 cggtttttgg agttcatggc ggccagggcg tcggcgacgg cggtttcctc
//
LOCUS       DAONFI010000344         2374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_58599_length_2374_cov_5.379474, whole genome shotgun sequence.
ACCESSION   DAONFI010000344 DAONFI010000000
VERSION     DAONFI010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58599_length_2374_cov_5.379474"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..733)
                     /locus_tag="PLK08_09565"
     CDS             complement(17..733)
                     /locus_tag="PLK08_09565"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPS53788.1"
                     /translation="MDRKLTRQEVRAIDRYAMERLGVSGLVLMENAGRNAADAIEGYL
                     ILRRSDANRLAVVAGGGNNGGDGFVIARHMAMRGFAVTIFIVVPRERIEKSADAWANL
                     RIAENFGLDVRFIDETAIAENLADELSLFHGIIDAIGGTGIVPPLTGPQALAAQAINR
                     AADANAEINVFAIDIPTGLDCDSGQADGSTVKADLTVSYVSQKAGFANDAAKAFTGDV
                     VTADIGIDSKAVIAMIDAEK"
     gene            complement(947..1279)
                     /gene="trxA"
                     /locus_tag="PLK08_09570"
     CDS             complement(947..1279)
                     /gene="trxA"
                     /locus_tag="PLK08_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPS53789.1"
                     /translation="MALEITDSNFEETVLNSGKPALVDFWAEWCMPCKMLAPTIEELA
                     KEYDGKVVIGKVDTDNNRGISMKFGISAIPTVIIFNAAGEMVKKFVGLQQKADFKAAL
                     DELLGATI"
     gene            complement(1419..2090)
                     /locus_tag="PLK08_09575"
     CDS             complement(1419..2090)
                     /locus_tag="PLK08_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53790.1"
                     /translation="MTEEMRKTTGKSASDLWNYSKPGISLWLGFGVLAYFGMMLFYYL
                     SMRGYQAEPHWLYDRRNHINQFLEDTSVFLPMAAAIIYWLKALWTRNMTYVVLAALCA
                     SLLLRELHWSHEIKVAIYPLLAMWLFWGIVWGDIMDKPSENPRHSRLLFAALFTFFLS
                     QLVEKKVFKFMPYGNTPLHTQYEEVVEFSGHLLIFLAAVFANIKRKKVENVYLQTDSR
                     RQMQP"
     gene            complement(2193..>2374)
                     /locus_tag="PLK08_09580"
     CDS             complement(2193..>2374)
                     /locus_tag="PLK08_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HPS53791.1"
                     /translation="LTAKAIGMKTVALTGKSGGELAKLADTAIRAPETETYKIQELHL
                     AIYHWLCMAVENEFF"
BASE COUNT          552 a          593 c          564 g          665 t
ORIGIN      
        1 aatatcagtt ggcggtttat ttttccgcgt ctatcatggc tatgacggct tttgagtcga
       61 tgcctatgtc agcggtgacg acgtcgccgg tgaaggcttt ggcggcgtcg ttggcgaagc
      121 cggctttctg cgatacatag ctgacggtaa ggtcggcttt tacggttgag ccgtcggctt
      181 ggccgctgtc gcaatcaagg cctgtcggaa tgtctatcgc aaagacattt atttcagcgt
      241 tcgcgtcagc tgcccgattt atcgcctgtg cggcaagtgc ctggggaccg gtgagcggcg
      301 gcactatgcc cgtgccgccg atagcgtcta ttatgccgtg aaaaagactc aattcgtcgg
      361 caagattttc agcgatagcc gtttcgtcga taaatcgcac gtcaaggccg aaattttccg
      421 caatacgcag attagcccag gcgtctgcgg atttttctat cctctcccgc ggaacgacga
      481 tgaatattgt taccgcgaaa cctctcatgg ccatgtgcct ggcgattacg aagccgtctc
      541 cgccgttatt gccgccgcct gcaacgacgg ctaacctgtt ggcgtcgctg cgacgaagga
      601 tgagataacc ttcgatggcg tcggctgcat ttctgccggc gttttccata agtaccagtc
      661 ctgatacgcc aagccgttcc atcgcgtaac ggtctatggc ccgaacttcc tgccgcgtaa
      721 gttttctgtc catgattttt accgtagcat attaaaatat ttaaaaaatg tcccaaaacc
      781 ttggttgcct gcaaaattgc tcgatttcgg cgtgtttttt aaaaaattgt cccaaaacct
      841 tggttgtctg caaaatggtt cgattttggc gtgttttttt taaaataaat acggcgttcc
      901 cgtgtttggc ggaaacgccg cgtgtttcta aaaaaacgtc aatttttcat atcgtcgcgc
      961 cgagcagttc gtccagggcg gctttgaagt cggccttctg ctggaggccg acgaatttct
     1021 tgaccatttc gccggctgca ttgaaaatga ttaccgtagg tattgcgctt atgccgaact
     1081 tcatggatat gccgcgattg ttatcggtgt ccactttgcc tatgacgact ttgccgtcgt
     1141 attccttcgc aagttcttcg atagtcggtg cgagcatttt gcagggcatg caccattcag
     1201 cccagaaatc caccagagcc ggcttgcctg agttcaaaac agtctcttca aagttggagt
     1261 ccgtaatttc aagtgccatt atccattctc cttgactaaa agctggaaac ttcaatacta
     1321 ccttccgcgt tagagcagtt ctctaaaaaa atttaaaagt cactctaatc gtaggcagct
     1381 aaataagaaa tgtcaaccat tttaagtgta gcgggaattc agggctgcat ttgccgccgg
     1441 ctgtccgttt gcagatagac gttttccact tttttgcgtt tgatgtttgc gaaaacggcg
     1501 gcgaggaaaa tcagcagatg gccggaaaat tccaccacct cttcgtattg cgtgtgtaac
     1561 ggagtattgc cgtagggcat gaatttgaac acttttttct ctaccagttg cgacagaaag
     1621 aaagtgaaca gggctgcgaa cagcaatctg ctgtgtcgcg gattttccga cggtttgtcc
     1681 attatgtcgc cccacacgat gccccagaac agccacatcg ccagtaacgg gtaaattgcc
     1741 acttttattt cgtggctcca gtgcagctcc cgcagaagca ggctggcgca cagcgcagcc
     1801 agtacgacgt aggtcatgtt tcgggtccaa agcgccttta gccagtaaat tatcgctgcc
     1861 gccatcggca gaaacacgga cgtatcttcc aaaaactgat ttatgtgatt gcgtctgtcg
     1921 tacagccagt gcggctcggc ctggtagcct cgcatggaaa gatagtaaaa cagcatcatc
     1981 ccgaaatatg cgagtacgcc gaaccccagc cagagcgaaa tgcccggctt tgaatagttc
     2041 cataaatcac ttgcagattt ccccgttgtt ttcctcattt cttctgtcat ttcatttccc
     2101 actcttaata ccctgatata actatgaaaa ctgttaaact atgtttaata aaagtctaaa
     2161 attaacttcg ttgcttgaaa agtcaaaaaa tatcagaaaa attcgttttc gacagccatg
     2221 cacaaccagt gatatatcgc cagatgtaat tcttggattt tatatgtttc cgtttccggc
     2281 gcacggatag ccgtgtcggc cagttttgcg agttctccac cggattttcc cgtcagtgcg
     2341 acggttttca tgccgatggc ttttgccgtc agca
//
LOCUS       DAONFI010000345         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_59147_length_2359_cov_2.963542, whole genome shotgun sequence.
ACCESSION   DAONFI010000345 DAONFI010000000
VERSION     DAONFI010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59147_length_2359_cov_2.963542"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1482)
                     /locus_tag="PLK08_09585"
     CDS             complement(316..1482)
                     /locus_tag="PLK08_09585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS53792.1"
                     /translation="MTPTILAILAALPSLIWISRHFRISRERKHGFVLTPEYAGPPSP
                     RPKISVIVAAKEEEENIRSCLETLAAQDYGDFEIIAVNDRSTDATGKIIDDFAAAHPN
                     VKAVHIKNLPDGWKGKNHAIHTAIPQATGEYFLFTDADCKMTSSRTLSVAMQLLKDHN
                     VGMLSVLPNLDTKSFWEDVIQPVCSAVLMLWFEPAKVNNPKKAVAYANGAFMLVSRHA
                     YDIIGGHAAIKNDIQEDMTMARHIKENHQGLFVTRNSGLYSVRMYRSLPEIINGWTRI
                     FYGSFRKLTRVLTTMFLLTFASVLPPAMVIIALLAYLGDPTHWLPCVITALASTSLEI
                     SVIYRYHKICGANKYLACMYPIGCLILDYILIRSSLKHRRGATIAWKNTSYTQK"
     gene            complement(1479..>2359)
                     /locus_tag="PLK08_09590"
     CDS             complement(1479..>2359)
                     /locus_tag="PLK08_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HPS53793.1"
                     /translation="SQVHNTTIDNIHFHEVGAVDSIIDIVTAAVALDELGIQDVFCSA
                     IPLGSGTIRCDHGIMPVPAPATARLLADAHAATVPGPNTGEATTPTAAAILTTLCKNI
                     GTMPAMTVTSTGYGAGSRQDGPAPNLLRVIIGETTSPDEGDCDCVLELSANIDDISGE
                     IIGAAIDSLMQAGALDAWASPIYTKKNRPAWTLGAICRLTDEKNIEELFFRCAGTFGL
                     RKKVVSRKKLHVEFESVETIYGPIRIKTGSLNGEIMAMKVEFCDALQAAQSHNATIIE
                     ITDTAKAAFLQKRNTQ"
BASE COUNT          488 a          550 c          675 g          646 t
ORIGIN      
        1 ggttatgatg ccactatgca taagtatgca tgttacacga catggtcggt ggatgatccg
       61 acaacttttg ttgatgaagc gttggaatgg tatgatttca cttccgtggc acaaggccag
      121 gtatttggca tatctgccgg tattactgtt gagattattg ctgcatgaga aatcggaacg
      181 aatgttttga tgccgtttgg catcaaaata cttgtaccgt ttatttcaaa agagttgatg
      241 ctgagaaata ttgttcccta tccattttat caaatgggta gggaactttt ttttatattg
      301 ccttgaaaag cggggttatt tttgagtata gctggtattt ttccaggcta tcgtagcgcc
      361 gcgacggtgc ttgagtgaac tgcgaatcag gatgtaatcc agtatcaggc agccgatggg
      421 gtacatgcag gccagatatt tgttcgcgcc gcagattttg tgatatctat aaattacgct
      481 tatctccagc gatgtgctgg ccagtgcggt gatgacgcac ggcagccagt gcgtcgggtc
      541 gccgaggtat gccagcagtg cgataatcac catggcaggc ggcaggacgc ttgcgaatgt
      601 cagcagaaac atcgtcgtaa gtacgcgcgt cagcttgcgg aacgagccgt aaaaaatccg
      661 cgtccagccg ttgatgattt ccggcagtga gcggtacatc cgcacagaat acaggccgct
      721 gttgcgcgtc acgaaaaggc cttgatggtt ttcctttata tggcgggcca tcgtcatgtc
      781 ttcctgtatg tcgtttttga tggctgcatg gccgccgatg atgtcgtagg catggcggct
      841 gacgagcatg aaagcaccat tggcgtaagc gacggctttt ttgggattgt tgaccttagc
      901 cggctcgaac cacagcatta aaactgcact gcataccggc tgaatgacgt cttcccagaa
      961 acttttggta tccaggttcg gcagaacgct gagcatgccg acgttgtggt ctttgagcag
     1021 ttgcatagcc accgacagcg tgcgtgatga tgtcatttta cagtcggcgt cggtgaatag
     1081 aaaatattcg ccggttgcct gcgggatagc tgtatgaatg gcgtggtttt ttcccttcca
     1141 gccgtcgggc aggtttttta tatgaaccgc cttgacgttc gggtgtgcgg cggcgaaatc
     1201 gtctattatt ttgcctgtcg cgtcagtgct gcggtcattg accgcgatga tttcaaaatc
     1261 gccgtaatcc tgggccgcca gcgtttcaag gcaggagcgg atgttttctt cctcttcctt
     1321 ggcggcgact attaccgaaa ttttcggacg gggtgacggt gggccggcgt attcgggcgt
     1381 gagcacaaaa ccgtgctttc gttcccgcga tatgcggaag tgcctgctta tccagattaa
     1441 cgatggcaat gccgccagta tggccaatat ggttggtgtc attgggtgtt ccgtttttgg
     1501 agaaatgccg cctttgcggt gtccgtgatt tcaataattg ttgcgttatg cgattgggca
     1561 gcttgcaggg catcgcagaa ttcgaccttc atagccatga tttcgccgtt caatgagccg
     1621 gtttttatcc tgatgggccc ataaatcgtt tcgacagact caaattcgac atgcagtttt
     1681 ttgcgtgaga cgactttttt acgcaggccg aatgtgccgg cgcatcggaa gaaaagctcc
     1741 tcgatgtttt tttcgtcggt cagccggcaa attgcaccaa gtgtccatgc cggccgattt
     1801 tttttcgtgt aaatcggcga tgcccacgca tccagcgcgc cagcctgcat gaggctgtcg
     1861 atggctgccc cgatgatttc gcctgaaatg tcgtcgatat ttgccgacag ttccagtacg
     1921 cagtcacaat cgccttcatc agggctggtg gtttcaccaa ttatcactcg cagcagattc
     1981 ggggctgggc cgtcctgtcg gctgccggcg ccgtaacctg ttgaggtgac tgtcattgca
     2041 ggcatcgtgc cgatgttttt acatagtgtc gtcaggatag ctgccgcggt aggggtggtt
     2101 gcttcgccgg tgttgggacc gggaacagtg gcggcatggg cgtcggctaa cagccttgcc
     2161 gtcgcaggag ccggcacggg catgatgccg tggtcacacc ttatcgtacc gctgccgagc
     2221 ggtatggctg agcagaaaac atcctgtatg ccgagctcat ccaatgcgac ggcggcggtg
     2281 actatgtcaa tgatgctgtc taccgcgccg acttcgtgaa aatgaatatt gtcaatggtg
     2341 gtattgtgaa cctgagatt
//
LOCUS       DAONFI010000346         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_59461_length_2350_cov_5.444009, whole genome shotgun sequence.
ACCESSION   DAONFI010000346 DAONFI010000000
VERSION     DAONFI010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59461_length_2350_cov_5.444009"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLK08_09595"
     CDS             complement(<1..900)
                     /locus_tag="PLK08_09595"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HPS53794.1"
                     /translation="MMRKKGIGVSPGVSIAQVVVVDNEEFDIPRRHIPPEQTTAEFER
                     LMRAVESSKTELRELQDRTTERIGEEASRIFDFHLGMLNDKLLFGKLEETIVSGCLTA
                     EYAVTTVLRAYASEFESMPEYLSERVKDVYDIERRLLRNLTGQQRQTLSHLTQNVIVV
                     THDLTPSQTASMDRKHIKGIAMDMGGPTGHTAIVARALGIPAVVGLENFTGNVNSGDN
                     LIIDGTTGLAILNPDEPTLQQYRKLAQAQYEFIHSLDSIASLPAITKDGHEIQLMGNI
                     EFPDESKTVLEKGGDGIGLYRTEF"
     gene            complement(1078..1365)
                     /locus_tag="PLK08_09600"
     CDS             complement(1078..1365)
                     /locus_tag="PLK08_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPS53795.1"
                     /translation="MAQVEKEITIINKYGLHARPAMQVVQLAQKYASNIEVAKDALKV
                     DAKSIMSVMRLAATKGTKLKISADGEDAQQAIDALTQLIADGFGELEPAGE"
     gene            complement(1434..1901)
                     /locus_tag="PLK08_09605"
     CDS             complement(1434..1901)
                     /locus_tag="PLK08_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPS53796.1"
                     /translation="MKLADIIVKDAICPQLKAATREEAISELVKALADAGAVTKNSVD
                     DVIKSVLQREKQATTGIGKGIALPHAKVLTVKRPIAAIGCATGGLDFSSLDSKPVYAV
                     ILMLSSPDKPDEHLQAMETIFKHVQRDMFRKFLYQAKTKQDVIDLMEEADEMV"
BASE COUNT          581 a          580 c          539 g          650 t
ORIGIN      
        1 gaattccgtg cgatacagcc caattccgtc accgcctttt tccagaacgg ttttggattc
       61 gtcgggaaac tctatgttgc ccataagctg tatttcatgg ccgtctttgg tgatagccgg
      121 cagagacgcg atggaatcaa ggctatgaat gaattcatat tgtgcctgag ccaatttgcg
      181 atactgctga agcgtaggct catcaggatt cagaatcgcc aagccggtcg taccgtctat
      241 tatgagatta tcgccgctat ttacgttgcc tgtaaaattc tctagtccca cgacagccgg
      301 tatgccaagc gcgcgtgcaa cgatagcggt gtggccggtc ggcccgccca tgtccatagc
      361 tatgcccttg atgtgcttgc gatccatgct agccgtctga ctgggcgtaa gatcgtgcgt
      421 gacgactatg acgttctggg tgaggtgcga aagagtctgt cgttgctggc cggtaagatt
      481 tctcaacagc cgccgttcaa tatcataaac gtccttgaca cgctcggaga gatattcggg
      541 catggactcg aattcactgg catacgcacg aagtacggta gtgacggcat attctgcggt
      601 aagacagccg gagactatgg tttcctccag tttgccgaaa aggagtttgt cgttgagcat
      661 tccgagatga aagtcaaaaa ttctggacgc ttcttcgcca atacgctctg tggtgcgatc
      721 ctgaagttcg cgaagttctg tttttgaact ttccacggct cgcatgagtc gttcgaattc
      781 cgccgtagtc tgttcagggg gaatatggcg tcttgggata tcgaattcct cgttatcgac
      841 gacaacgacc tgggctatcg aaacgccagg cgaaactccg ataccttttt ttctcatcat
      901 agatttgcta tcgtactcta ccaccttttt aaatccgtaa gccctctgag ggccgcctcc
      961 gttatcgctc ggattgacgc cagcaattag cggcggcatc atttatccga tagaccctga
     1021 attctgttat aaatataaaa tacaaaaatt tttacagccg ctcaaatgaa gcggcggtta
     1081 ttcacccgcg ggttccaatt cgccaaaacc atccgcaata agctgggtca gcgcatcaat
     1141 cgcctgctgg gcatcctcgc cgtcggctga tattttaagt tttgtgcctt tcgtggcagc
     1201 cagccgcatg accgacatga tgctcttggc atcaaccttc agcgcgtctt tggcgacttc
     1261 gatattgctg gcatatttct gggcaagctg tacgacctgc atcgccggcc tggcgtgcaa
     1321 cccgtacttg tttattatcg ttatttcttt ttcaacctgt gccacgaaaa aacactccgg
     1381 taaacaatat cgccggaatc ctcaaaaaca gttgatagtt cgttaaaaag gaatcagacc
     1441 atctcgtcgg cttcttccat caggtcgatg acatcctgtt tcgttttagc ctgatagaga
     1501 aacttcctga acatgtcgcg ctgcacgtgt ttgaaaatag tttccatcgc ctgcagatgt
     1561 tcgtcgggct tatccgggct tgacaacatc agtattacgg cgtaaacggg cttggaatca
     1621 aggctggaaa aatccaggcc cccggttgca cagcctatcg cggcaatagg tctttttaca
     1681 gtaagcactt tggcgtgcgg cagtgcaata cccttgccta tgcctgtagt tgcctgcttt
     1741 tcccgctgga gaacgctctt gattacgtca tcaacggaat tcttggtaac cgcgccggcg
     1801 tcagccagtg ctttgaccaa ttctgaaata gcttcttcac gtgtagcagc tttcagttgt
     1861 ggacatatcg catctttaac tataatatct gcaagcttca taatcagctc ctgtcgtggc
     1921 acatttactt gtaaaaaagt cgtcgagcct gacatcgtcc gctgattgca acaggtgtga
     1981 cgtaatcgca ggttcaacag cttggacaag tttacaaaaa aatattccgg aattccagtg
     2041 tccatatgga tttgtcatga aatttcattc ataatttgtt ttcaaacctg ctgccagccg
     2101 taaaagaaca cgcctcaaga aaaacagtca aaatatcctc tcttgcctct cataaaatct
     2161 ggaaataaga aaaaggccga cagttttggc ccaattcata aaaaaacggc tgataaaatg
     2221 aaaataaacc acttttcaaa aatttttcca tataaacggc acgacataga aggggaattt
     2281 ttttgaaaaa atcttttcat gcgtgtgatt agcgccttgt ggccggcttt tacgtgtaac
     2341 cgtcggctac
//
LOCUS       DAONFI010000347         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_60112_length_2334_cov_3.932865, whole genome shotgun sequence.
ACCESSION   DAONFI010000347 DAONFI010000000
VERSION     DAONFI010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60112_length_2334_cov_3.932865"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="PLK08_09610"
     CDS             complement(<1..1312)
                     /locus_tag="PLK08_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS53797.1"
                     /translation="MNRQNDTSYTAAAEHMTAAQVGKVYQHEFSPSEDADKSPARTSD
                     GYLTSAEYLRAAMKNISADSTADAIKLNFHHPVEDWLRKKWVRRILGAISRPDEKGHT
                     RLERILRSYRNPHAGVWSRIKYLPIHFAISRLVGPKGWDDFRHRSKQFGATVRGLNVV
                     ARSIAEFGLTVPQKFTSPLFVVWNFTNICNLRCKHCYQYSEHSTLADELTLEEKLDFV
                     DQMGRLNIPMLAISGGEPTLSKDLIPVIKRAREYDMHISLATNGTTMTPQRAVELRDA
                     GLRYVEISLDSTKPERHDAFRGIAGMWEKTVQGMRNVVATDGLRLGVAMCVHQGNIDE
                     VRDMIKFAEEEIGASCLAHFNFIPVGRGLKMVSGDITPSQREELLAILNEKMQAGGMG
                     IISTAPQLGRVCLAGAALDDGVVTCSHAGGGSGVKARVVAKYLGG"
     gene            1523..2332
                     /locus_tag="PLK08_09615"
     CDS             1523..2332
                     /locus_tag="PLK08_09615"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPS53798.1"
                     /translation="MKLTLGRKLAAIFCGVGMICIAMICAGCEVDAAERYMTDVRKEN
                     GLVIILPGIEGESEFNHNIRRGLVSAGSYRAMPIYNWGRPIPGLGMLLNQMDVIGNRA
                     AGRRIAQMIEQYEDAYPGRPVYIIGHSGGGGVAVFAAEELSKDYKIDGLVLIAASISD
                     NYNLNKALKSCRNGIVNFYNPEDAGLLGVGTTVMGNVDGGHGPSAGLNGFNKNYNNLY
                     QVKVTESMTAASGGGSHDAATRPRFVATYVAPWVLSSRWPPSTLASYNPYR"
BASE COUNT          506 a          602 c          681 g          545 t
ORIGIN      
        1 agccgccgag atacttggcc accacgcggg ctttgactcc gctgcctccg ccggcgtggc
       61 tgcacgttac cacaccgtca tccagcgcgg caccggcaag gcagacgcgt ccaagctgcg
      121 gtgcggtgga aattatgccc atgccgccgg cttgcatttt ttcattcagt atcgcgagca
      181 gttcctcacg ctgcgacggg gtgatgtcgc cgctgaccat cttcaagccg cggccgacgg
      241 gtatgaaatt aaaatgagcc aggcagcttg caccgatttc ttcctcggcg aattttatca
      301 tatcgcggac ttcgtcgata ttgccctggt gtacgcacat ggcgacgccc agccgcaggc
      361 cgtccgtggc gacgacgtta cgcatgccct ggacggtttt ttcccacatg ccggcaatgc
      421 cgcggaatgc gtcgtgccgt tccggctttg tggagtcgag cgatatttcc acgtatcgca
      481 ggccggcgtc gcgcagttcg acggcacgct gcggcgtcat ggttgtgcca ttcgttgcta
      541 acgaaatatg catgtcgtat tcgcgagccc gctttatgac agggatgagg tccttgctga
      601 gagtcggctc gccgccggag atggctagca tgggaatgtt cagccggccc atctggtcta
      661 cgaagtcgag cttttcctca agcgtaagtt cgtcagccag cgtgctgtgt tcggaatact
      721 ggtaacaatg cttgcacctg agattgcaga tgttggtgaa attccagacg acgaacagcg
      781 ggctggtgaa tttttgcggc acggtaaggc cgaactcggc aatcgagcgg gctaccacgt
      841 tcaagcctcg cacggttgcg ccgaactgct ttgaacgatg acgaaaatca tcccagcctt
      901 ttggaccgac cagccgactt attgcaaaat ggattggcag atattttatc ctgctccaga
      961 cgccggcgtg cggattacgg taactgcgca aaatacgttc cagccgtgta tggccctttt
     1021 cgtccggacg cgatatcgcg ccgagaatgc ggcgaaccca tttttttctc aaccagtctt
     1081 cgacgggatg atgaaaattc agcttgatgg cgtcagccgt tgaatcggcg gagatatttt
     1141 tcatggctgc gcggagatat tcggcgctgg tgagatatcc gtcggacgtt cgtgcaggag
     1201 atttgtcggc gtcttccgac ggcgaaaatt cgtgttgata gacttttccg acctgggctg
     1261 cggtcatgtg ctcggctgcg gcggtgtacg aagtatcgtt ttgtctgttc atgcgtacct
     1321 cttgttggaa gctacacgat attattaaac ggcgacgatg tcaattgtct ggctgaatgt
     1381 atttttcagg gacaaaacag aagatttatt ctggcaaatc gtcggggcga gagctaaaat
     1441 gggctcgtta tgcggtttgg gatgttgcga cgattttacc ggtttgtacc agactaaaaa
     1501 gggctaaaga gaggacagga taatgaaatt gactctcgga cgaaagttgg cggcgatttt
     1561 ctgcggcgtg ggcatgattt gcatagccat gatttgcgcc ggctgtgaag tggatgctgc
     1621 cgaacgctac atgaccgatg ttcgcaagga gaacgggctg gtcataattc tgcccggcat
     1681 agagggcgaa agcgaattta accacaatat ccgccgcggg ttagtatcgg ctggaagcta
     1741 ccgtgcaatg ccaatctaca actggggtcg gccgataccc ggcctgggca tgctgctcaa
     1801 ccagatggat gtaatcggca atcgtgccgc cggcagacga atcgcccaga tgatagaaca
     1861 atatgaagat gcatatcctg gccggccggt ttatatcatc ggccacagcg gcggcggcgg
     1921 cgtggcggtg tttgcagcag aggaactttc gaaagattac aagattgacg gcctggttct
     1981 tatcgccgca agcatatcgg acaattataa tctcaataag gcactcaaaa gctgccgtaa
     2041 cggcatcgtg aatttttaca atcctgagga tgccggcttg ttgggcgtgg gcacgactgt
     2101 catgggcaac gtagacggcg gccatgggcc gtccgccggc ttgaacggtt ttaataaaaa
     2161 ctacaacaac ctctaccagg taaaagtcac cgaatccatg acagccgcca gcggcggcgg
     2221 ttcgcacgat gcagctacgc ggcccagatt cgtcgcgacg tatgtggcgc cgtgggtgct
     2281 gtcaagccgt tggccgccaa gtacgctggc atcctacaat ccgtatagat aatc
//
LOCUS       DAONFI010000348         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_60596_length_2322_cov_3.844288, whole genome shotgun sequence.
ACCESSION   DAONFI010000348 DAONFI010000000
VERSION     DAONFI010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60596_length_2322_cov_3.844288"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLK08_09620"
     CDS             <1..369
                     /locus_tag="PLK08_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53799.1"
                     /translation="KLIALQDSAQRLSPEDAANITAELDIVDKFMAAPDKFAPQVSLY
                     PMAGVTPGCCGLLLTPNTSTAVITGPAVPTRNHMERGMVWEECLDLEEALKAMSELYE
                     LADIIIPGFDNICFSPGRMF"
     gene            454..1749
                     /locus_tag="PLK08_09625"
     CDS             454..1749
                     /locus_tag="PLK08_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPS53800.1"
                     /translation="MATLDAGFCAESCAEVAALCVLLCASAFFSGTETALFVLTRVEL
                     RELSQGGSWAGKLVGRLMKRPERVLNTLLLGNMIVNVAFASISAMLLLRLQNERDLPA
                     WVVAAGSLCPLAAVIFFGEVTPKMLAYSVAGRWAKLSAWPVFLLQQILRPAIWVMEFF
                     IVKPATKIILPSGRTPETIRPEEMAAMMELSASRGNLDSDAGEMLREIVWLSQKKVRD
                     VMVPRVDVIQFDVNDPPEELDAMFRKTHLRKIPVFDARKDHTLGAVRAREFLLRKGEN
                     SIRDFIRPVIYIPENANLTQALDTFRRTSKQMAIVVDEYGGTAGLVTLEDILEEIVGD
                     IPDRHELPEKPVEKISDSQYLVSGELPVHDWPAAFGLKQFDKRISTVGGLVMLLLERL
                     PLTGDSVVLGNLRLTVREMRNNRVETVLIDVGGRKGAVK"
     gene            1746..>2322
                     /locus_tag="PLK08_09630"
     CDS             1746..>2322
                     /locus_tag="PLK08_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HPS53801.1"
                     /translation="MTAVTTFRDWLIWGGLAAFGVMMVGIYCSLETSLYVLNRIRLEL
                     KSQTGNRRAKYLRAMYRKPNQLLTVLLIGTNVFSYLATFAVSILVLHVGIPDDRAEFY
                     TIAIAAPLLFAFSDAVPKNVSRIIAERIAYGLCWVLRISDAVFTFTGVRPLLQVLSGM
                     WVRLLGRKSGQTDMGLAAVISEAHASGVITSS"
BASE COUNT          500 a          599 c          668 g          555 t
ORIGIN      
        1 aaactcatcg cactgcagga ttccgcacag cgattgagcc ccgaagatgc cgcgaatatt
       61 accgccgaac ttgacatagt cgacaaattc atggctgccc ccgataaatt cgctccgcag
      121 gtcagccttt atccgatggc tggcgtgacg cctggctgct gcggtctgtt gctgacgccg
      181 aatacttcga cggctgtcat aaccggcccg gctgttccga cgcgcaacca tatggagcgg
      241 ggcatggtct gggaagaatg tctcgatttg gaagaagcac tcaaagccat gtctgaactt
      301 tatgaactgg cggatataat aattccaggt tttgataaca tctgtttttc tccggggcga
      361 atgttctgac atgaatgcgt cgtatggaaa agaagattgc ggcggctctc ggttagagca
      421 tatgggcgga aaggctgggc ggccgctgtg ataatggcaa cgctggatgc cggtttctgt
      481 gctgaatcat gtgcggaggt tgccgcatta tgcgttctgc tgtgcgcgtc ggcatttttc
      541 agcggcaccg agacggcgtt gtttgtccta acccgcgttg agttgcgcga actttcgcag
      601 ggcgggagct gggccggcaa actggtgggg cggctgatga aaaggcccga acgtgttctc
      661 aatactctcc tgctcggcaa tatgatagtg aatgtcgctt ttgcgtccat ttctgcgatg
      721 ctgctgcttc gtttgcagaa tgaaagggat ttgccggcct gggtggttgc agccggcagt
      781 ctgtgtcctc tggcggcggt gatttttttc ggcgaagtga cgcccaaaat gcttgcatac
      841 tccgtagccg gccgctgggc gaaattatcg gcatggccgg tatttctgct ccagcagatt
      901 ctgcgaccag ctatatgggt tatggagttt ttcatagtca aaccggctac caaaatcatc
      961 ctgccgtccg gccgaacgcc tgaaactatc cggccggagg agatggctgc catgatggaa
     1021 ctctccgcca gccgcggcaa tctggactcg gatgccggcg aaatgcttcg cgaaatagtc
     1081 tggctgtcgc aaaaaaaagt gcgggatgtc atggtgccgc gcgttgatgt catccaattc
     1141 gacgtaaatg accccccaga ggaactggac gccatgttcc gcaagacgca tctgcgcaaa
     1201 ataccggttt tcgacgccag gaaagatcac accctcggtg cggttcgtgc ccgcgaattt
     1261 cttcttcgca agggcgaaaa ttcaatacgg gacttcatac ggccggtgat ttatattccg
     1321 gaaaacgcaa atcttacgca ggctctggac acattccggc gcacaagcaa gcagatggct
     1381 atcgtggtcg atgaatatgg cggaacagcc ggccttgtta cgcttgaaga tattttggaa
     1441 gaaatcgtgg gcgatatccc tgaccgtcat gaactgccag aaaagcctgt ggaaaaaatt
     1501 tccgattcgc aataccttgt cagcggcgaa ctgccggtac atgattggcc ggcggcgttt
     1561 ggcctaaaac agttcgacaa acgcataagt accgtcggcg gccttgtgat gctgctgctc
     1621 gaacgtctgc cgctgaccgg cgacagcgtg gtgctgggta atctgcggct gacagtcagg
     1681 gaaatgcgga acaatcgcgt tgagacggta cttatagacg tcggcggcag gaagggggct
     1741 gtaaaatgac agccgtcact acttttcgcg attggctgat atggggcggc ctggctgctt
     1801 tcggcgtaat gatggtcgga atatactgca gccttgaaac cagtctgtat gtgctcaatc
     1861 gtattcgcct tgaacttaaa agtcagaccg gcaacagacg ggcgaaatat ctgcgggcaa
     1921 tgtaccgcaa gccgaatcag cttttgactg tactactcat cggcacgaat gttttttcgt
     1981 acctggcgac gtttgccgtg agtattcttg tcctgcacgt cggcatacct gacgaccgcg
     2041 cggaatttta caccatcgcc attgccgcgc cgcttctgtt cgccttcagc gacgctgttc
     2101 cgaaaaatgt ttcgcgtatc atagctgagc gcatcgccta cggcctttgt tgggtacttc
     2161 gcatatccga tgccgtattc acgtttaccg gcgtccggcc gctgcttcag gtgctcagcg
     2221 gcatgtgggt caggctgctt ggccgcaaaa gcggccagac ggatatggga cttgcggcag
     2281 ttatctccga agcccatgca agcggcgtga taacttccag cc
//
LOCUS       DAONFI010000349         2306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_61256_length_2306_cov_3.941804, whole genome shotgun sequence.
ACCESSION   DAONFI010000349 DAONFI010000000
VERSION     DAONFI010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61256_length_2306_cov_3.941804"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLK08_09635"
     CDS             complement(<1..652)
                     /locus_tag="PLK08_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53802.1"
                     /translation="MKRERIKQALADKDYSAAEDLWMDAIVDSVSPTFASFVLDAFMQ
                     AGRTTSAETMGWALLDAHKKSPDEKQLSLAKAVLLAVPASEELRKITSELYRKVNSGR
                     ENFDMLWKAADLEGKQRPQRALRTLDTCLDVKPGSYMANRYNNSVIRIERLNAMDEYE
                     YTGDDGDGSLDPKKLADEYEVLGDDDFRVLKRVNPQGMSDTFAENPLKALVSICKSR"
     gene            complement(683..2140)
                     /locus_tag="PLK08_09640"
     CDS             complement(683..2140)
                     /locus_tag="PLK08_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HPS53803.1"
                     /translation="MKDYKSIKEILEKHSQQHLLKFFDELGPDHQQLLLEQLSEIDFD
                     FMDKLTREYVFDKPAVEVPRDMTPPQTIPASPKPGLPLTGIYAQARKLGEKLISEGKV
                     AAFTVAGGQGTRLGYEGPKGCFECTPVVNKPLFRLFAEQIRSASLRFGKTIPWYILTS
                     PTNDTATQAHFRQNDYWAFDPKDIIFVKQGTMPAIGYDGKLLLGRKYRLAVSPDGHGG
                     SLTALRHSGALADMARRGIEYISYFQVDNPLVRAIDPIFIGLHAMNGAEMSAKSLPKR
                     EPLEKLGNFCVVDGKTCVIEYSDMPQSLAMACNQDGTLKFSAGSIAIHIFSRAFMEKL
                     TDGGKCMLPFHRADKKVPYVNDAGELVKPENENAVKLEMFVFDAMPLAEKTVIYETRR
                     EEEFSPIKNAEGNDSPATCLHDQVRRWASWLDAAGVKIPRDASGLVAAAIEISPLFAN
                     SADELADKLKKLGKEITIAPGQNFYLGSKGQIGGA"
BASE COUNT          503 a          612 c          591 g          600 t
ORIGIN      
        1 cgcgggattt gcatatcgat accagcgcct taagcggatt ttcggcaaat gtatcagaca
       61 tgccctgcgg attgacccgc ttcagaacac gaaaatcgtc atcgccgaga acttcatatt
      121 cgtcggcaag ttttttggga tcgagcgaac catctccgtc gtcgccggtg tattcgtatt
      181 cgtccatggc gttgagccgt tctatacgaa tgacggagtt gttatagcga ttagccatat
      241 aactgccagg cttgacgtca agacatgtat ccagcgtacg aagggcccgt tgcggacgct
      301 gtttgccttc aagatcggcg gctttccaga gcatgtcgaa attttccctg ccggaattca
      361 ccttgcgata aagttccgag gtgatttttc gaagttcttc gctggctggc actgcaagca
      421 ggacggcctt tgcgaggctg agctgttttt catccggcga cttcttatgg gcgtcaagca
      481 gcgcccagcc cattgtttca gccgaggttg tccggccagc ctgcatgaac gcatccagca
      541 caaacgacgc gaacgtcggc gaaacgctgt ccacgatggc atccatccac aaatcttcgg
      601 cggcactgta atccttgtct gccagtgcct gtttgattct ttctcttttc atttgtaaat
      661 ctgttcttcc tgctgatact gcttatgctc cgccaatctg gccttttgag ccgagataga
      721 aattctgtcc cggcgctatc gtgatttcct tgccgagttt tttcagctta tcggcaagtt
      781 catccgcact gttggcgaat agtggtgaaa tttcaatcgc agccgccacg aggccactcg
      841 cgtcacgagg tattttgacg ccggctgcgt ccagccagct tgcccagcgc cgcacctgat
      901 cgtgcaggca ggtagccggc gaatcattgc cctccgcatt ctttatgggg ctgaactctt
      961 cttcacgacg tgtttcatat ataaccgtct tttccgccag cggcatggcg tcgaaaacga
     1021 acatctcaag tttgacggcg ttctcatttt caggcttgac gagttcgccg gcgtcgttga
     1081 cataaggcac tttcttgtcc gcgcgatgga acggcagcat gcatttgccg ccgtcggtga
     1141 gtttttccat aaaagcgcgg ctgaagatgt gaatcgcaat cgagccggct gaaaatttca
     1201 gtgtaccgtc ctgattgcac gccatcgcca gcgattgcgg catatcggaa tattcaatga
     1261 cgcaggtttt gccgtcgact acgcagaagt tgccgagctt ttcaagcggc tcgcgtttgg
     1321 ggagcgattt ggctgacatt tcggcgccgt tcatggcgtg caggccgatg aatatcgggt
     1381 cgatagccct gacgagcgga ttgtcaacct ggaaatagct gatatattct atgccgcgcc
     1441 tggccatgtc cgccagtgca ccgctgtgac gcagcgccgt cagactgccg ccgtggccgt
     1501 ccgggctgac cgccagacga tatttgcggc caagcagcag tttgccgtcg tagcctatcg
     1561 caggcattgt gccctgcttg acaaaaatga tgtccttggg atcgaacgcc caataatcgt
     1621 tctggcgaaa atgcgcctgc gtggcggtat cattagtagg gctggtgaga atgtaccacg
     1681 gaatggtttt accaaaacgc aaagacgccg agcgaatctg ctcggcaaaa agacggaaca
     1741 gcggcttgtt gactaccggc gtacattcaa aacacccttt ggggccttca tagcccagcc
     1801 gcgtaccctg gccgccagct accgtaaagg ctgcaacctt accttccgag ataagttttt
     1861 cgccgagttt gcgggcttga gcgtaaatgc ccgtaagtgg caggccgggc ttgggagagg
     1921 cgggaatggt ttgcggaggc gtcatgtcac gcggcacttc cacggcaggc ttgtcaaaca
     1981 cgtattccct ggtgagtttg tccataaaat caaaatctat ttccgaaagc tgttcaagca
     2041 acaactgctg atgatccggc ccgagttcat cgaagaactt caacaagtgc tgctgcgaat
     2101 gtttttcgag tatttctttt atgctcttgt agtccttcat atgtttgtct ccaatttttt
     2161 aatatgtcct tcgtaattct acggtctttc aaaaaaaaga cccgcagatg aaagcatccg
     2221 ccatcagacg gacaggcaat aaccttaaaa ctaaatacaa cctatgaccc gtagtaattc
     2281 atttatcgtg tattattacg acctgt
//
LOCUS       DAONFI010000350         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_61934_length_2289_cov_4.127574, whole genome shotgun sequence.
ACCESSION   DAONFI010000350 DAONFI010000000
VERSION     DAONFI010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61934_length_2289_cov_4.127574"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..807)
                     /locus_tag="PLK08_09645"
     CDS             complement(193..807)
                     /locus_tag="PLK08_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPS53804.1"
                     /translation="MNVSRRELMVFGLLAALAVCVMSLTNTVAQSSPAKVGPTSVAVC
                     NIMEIFTKYNRATDLMKELEKKQQAIQSENEKKTKAIEAAQQELGSYNPGKPEYQRVQ
                     EKIKRLAIERQVWLRMQENSLLEEHLNLTKDMYEEVRKAVAAVAKYNGVDIVLQIEPN
                     DFSVENATQMVNLISTRKVVYSADRLDLTPSVLQYLNMAYRAKP"
     gene            complement(974..>2289)
                     /locus_tag="PLK08_09650"
     CDS             complement(974..>2289)
                     /locus_tag="PLK08_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPS53805.1"
                     /translation="MLLPFVLQSAEVMRRSVELLQPYMNSAGGEEKTVKILLATVAGD
                     VHDIGKNLVDIILSNNGYKVYNIGIKMPAEEIIAKAREYDVDVIGLSGLLVKSAMTMK
                     ADMPIYANAGLTQPILLGGAALTQQFVACECVPNYTTGKVVYCSDAFDALRAMHRFEA
                     GTLESSSWSAQQAAPQTKAGEFSISHDNPVPQPPFVGKRIITNWTLDDILPYLNEQVL
                     FRVRWGYRMGKLSRNEYEDMLANEVRPLFDSLIRRCREERIITPMVSYGYFSCASDGE
                     KLTVSSADGTGQSVFTFPRQADAPHMCVADYFHTARSGGDILPGFVVTVGESILNEAR
                     RLYEADEYRDYLLLYALGMELAEAMANRIHRHIRRELGLDEKTSARYGFGYSSCPDLS
                     LQAELFRLLGADEIGLRLTESFEIVPELSTAAIVACHPQARYFTV"
BASE COUNT          523 a          653 c          557 g          556 t
ORIGIN      
        1 ctgcgcggcg ggcatcggtg gcgaacgtga cggactgcgg cgtggcgctt tccagcgacg
       61 ctatttcgca gacgctcaat tcgccgtcgc cttcgagacg gcctgcgagc agttcagcca
      121 gtctggcagc ttttaatggc gtttcaaaca tgcaatgcta tctttcgtta atcgggctga
      181 ttggcggctg tatcacggct tggcgcgata agccatattc aggtattgca gaaccgacgg
      241 cgtcagatcc aaccggtcgg cggaatacac aactttcctt gtgcttatga gattgaccat
      301 ctgagtggcg ttttcaacgc tgaaatcgtt cggctcaatc tgcagcacga tatcgacgcc
      361 gttatacttg gccaccgcgg cgacagcctt gcgaacttcc tcatacatat ctttggtgag
      421 attcagatgc tcttccagca gtgaattctc ctgcatgcgc agccagacct gacgttcgat
      481 ggcaaggcgt ttgatttttt cctgcacgcg ctgatactca ggcttgccgg ggttgtagct
      541 gccgagctcc tgctgggcgg cttcgatagc cttggttttc ttctcatttt ccgactggat
      601 agcctgctgc ttcttctcca gctctttcat aagatctgtc gcacgattgt atttggtgaa
      661 tatctccatg atattgcaca cggctacgct ggtcgggccc accttcgccg gcgaagactg
      721 agccacggta tttgtcagcg acattacaca tacggctaac gccgccaaca gtccgaaaac
      781 catcaattct ctgcgtgaaa cgttcatttc gtaatccttt ttaaaaaact tccgtcaagc
      841 tgtcaacaaa tccaacaagc gtaatagttt accgcacaac gcggcggcaa tcaactaatc
      901 agacgttttc catcgggcaa aatcctgtca ggcagccttc gcaaaatcaa agcgtgaatt
      961 ctttaaaatt ccgtcagact gtaaaatatc tggcctgcgg atgacacgca actatcgctg
     1021 ccgtcgatag ttccggcaca atttcaaaac tctccgtcag ccgcaatcct atttcatccg
     1081 caccaagcag ccggaaaagc tcagcctgta aagataaatc cggacacgac gaataaccga
     1141 aaccatatct ggctgacgtt ttttcatcca gccccagttc gcggcgaata tgcctgtgta
     1201 tccggttggc catcgcctcc gccaactcca tgcccaacgc ataaagcagc agataatcgc
     1261 gatactcgtc agcctcgtat aaccgcctcg cctcattcag aatactctcg cccacggtaa
     1321 cgacgaaacc aggcagaatg tctcctccgc ttctggccgt gtgaaaataa tcggctacgc
     1381 acatatgcgg cgcgtcagcc tgccgcggga acgtaaaaac cgattgaccc gtcccgtcgg
     1441 cggaactgac cgtcagcttc tcgccgtcgc ttgcacaact gaaataaccg taactgacca
     1501 tcggcgtgat tatgcgttct tcacggcagc gacggataag gctgtcaaac agcggacgta
     1561 cctcattagc caacatgtct tcatattcgt tccgcgagag tttgcccatc ctgtatcccc
     1621 atcgcacacg gaaaagcacc tgttcgttga gatagggcaa tatatcgtcc agcgtccagt
     1681 tcgtaataat tcgtttgccg acaaacggcg gctgaggtac aggattgtca tgcgatatcg
     1741 aaaattcgcc ggccttcgtc tgcggagcag cctgctgcgc agaccagctg gacgattcga
     1801 gcgtgcctgc ctcaaagcgg tgcatagccc gcaaggcatc gaacgcatcg ctgcaatata
     1861 cgacttttcc ggtagtataa ttcggcacac actcgcaggc tacaaactgt tgagtgagag
     1921 cggcgccgcc gagcagtatc ggctgcgtga ggccggcgtt ggcgtagata ggcatgtcgg
     1981 ctttcatggt catcgccgat ttcaccagca ggccgctgag gcctatcacg tcaacatcgt
     2041 actcgcgagc cttggcaatg atttcctcag ccggcatttt tatgccgata ttatacacct
     2101 tgtaaccgtt attactcaaa atgatgtcaa cgaggttttt gccgatgtcg tgtacgtcgc
     2161 cggctacggt agccagtagg attttgacgg ttttttcctc gccgccagca ctgttcatgt
     2221 acggctgaag aagttccacg ctgcgacgca tgacttcggc ggactgcaac acgaacggca
     2281 gcagcattt
//
LOCUS       DAONFI010000351         2236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_64287_length_2236_cov_2.782669, whole genome shotgun sequence.
ACCESSION   DAONFI010000351 DAONFI010000000
VERSION     DAONFI010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64287_length_2236_cov_2.782669"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..865
                     /locus_tag="PLK08_09655"
     CDS             344..865
                     /locus_tag="PLK08_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPS53806.1"
                     /translation="MNRFVEMILSRCSVREFTGESVKKCDLEEMLKAGMSAPSAVNIQ
                     PWAFVVITDKSKMQNICRQLPYAKMLVNAGAAIVVCGLPGKDAQFAPKFWPLDCSAAT
                     ENILLAAHALGYGAVWTAVYPEADRIEAVRRECHLPQDVIPLNVIPIGVPSQRSAKPI
                     DKFKPDNIHWDNW"
     gene            complement(884..>2236)
                     /gene="cls"
                     /locus_tag="PLK08_09660"
     CDS             complement(884..>2236)
                     /gene="cls"
                     /locus_tag="PLK08_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPS53807.1"
                     /translation="LGFFLAIILISHLIRQRRSPSGTMAWLMGLILIPHIAVPLYLIF
                     GGRKMRYKQSLKQNISFPGMTTALVDHEARRLSELISSFGMPLPARGHRITLCSDSCE
                     TYHKLMELLESAEKSIDFATYVFEHDATGQSVLEVLTRKARSGVNVRLLVDDIGAHML
                     KRRHVKDFIAAGGQFARFMPLVHITRRADLRNHRKITLIDDSTVMAGGMNIGDEYMGP
                     TFNPSRWKDLSFVMQGPCVGVYGEIFRQDWLFATGRQDKDEQITPDGQPHFEEDQIAQ
                     VVPSGPDVKGDILYNALVAAIHAAEERVSIITPYFIPDDSMMQSLTLAAHRGVNVEVI
                     VPQVSNQRLANLARGPYLRDLTAAGVKVRYYLPGMMHAKVVLIDDDVAMVASANFDLR
                     SMQLNYEVAMFAYSAQTINAVAAWVDGLRRQTIANTPLQIPYLRETVEGVARLLAPLL
                     "
BASE COUNT          508 a          566 c          616 g          546 t
ORIGIN      
        1 tcgtaacaag gtagccgtag gggaacctgc ggctggatca cctcctttct agaggataca
       61 tctaggtgtc gatggctgga tttgcagttt cacgactgta tgatttcagc cggaagagta
      121 atcttcccga cataattaaa gatgtcagca cagcctcgtc cggcgtagcc ggatgactgc
      181 atggcgacat gcaactaaga caactcactg tttcgatatt ttgaagagcc gccggaataa
      241 actccggcgg ttttttcatg cgcatttttt ttataatcgc tttaaaataa tgcatgatta
      301 tattacatgt tatggtgatt ctattttttc gggagtaagc attatgaaca gatttgttga
      361 aatgatactt tcgaggtgta gtgtgaggga gtttacgggc gagtcggtga agaagtgtga
      421 cttggaggaa atgttgaagg cgggaatgtc ggcgccgtca gccgtcaaca ttcagccttg
      481 ggcgtttgtg gtcattacag acaaatcaaa aatgcagaat atttgccgcc aacttcctta
      541 cgcaaaaatg ctcgtcaacg ccggtgccgc cattgttgtc tgcggcctgc caggtaagga
      601 cgcccaattt gcccccaaat tctggccgtt ggattgctcc gccgccacgg aaaatattct
      661 tctcgccgct catgctctcg gttacggcgc cgtctggact gccgtctacc ctgaagcgga
      721 cagaattgag gctgtccggc gcgagtgcca tctcccccag gatgtcattc cactgaatgt
      781 gattcccata ggcgtgcctt cccaaaggtc cgccaagccc atagataaat ttaaacctga
      841 caatattcat tgggataact ggtaacgaaa gttttttgat atcttacagg agtggggcga
      901 gcaatcgtgc gacgccttct acggtctcgc gcagatatgg aatttgcagc ggcgtatttg
      961 caatggtttg ccggcgaagt ccgtctaccc atgccgccac ggcattgatg gtttgggcgg
     1021 aatatgcgaa catggccact tcatagttca gctgcatgct gcgcaaatca aaattggctg
     1081 acgcgaccat ggctacgtcg tcgtcgatga ggacgacctt ggcgtgcatc atgccgggca
     1141 gataatatcg cacttttacg ccggcggctg tgaggtctcg cagatacggc ccgcgtgcca
     1201 ggttcgccag ccgctgattc gacacctgcg gcactatcac ctcgacgttt acgccgcgat
     1261 gtgccgccag cgtaagcgac tgcatcatcg agtcgtcggg aatgaagtag ggcgttatta
     1321 tgcttacgcg ttcttcggcg gcgtggatag ctgccacgag tgcattatac agtatgtcgc
     1381 cctttacgtc ggggccggag ggcactacct gggcaatctg gtcttcttcg aaatgcggct
     1441 ggccgtcagg ggtgatttgt tcatccttat cctgcctgcc agtggcgaac agccagtcct
     1501 gccggaaaat ttcgccataa acgcccacgc agggcccctg cattacgaaa gacaaatctt
     1561 tccatcgcga aggattgaac gtcggcccca tatattcatc gccgatattc atgccgccgg
     1621 ccatcacggt cgagtcgtct atcagcgtaa ttttgcgatg attacgcagg tcggcacggc
     1681 gggtgatatg taccagaggc atgaatctgg caaactggcc gccggcggcg atgaagtctt
     1741 tcacgtgccg ccgcttgagc atgtgtgcgc ctatgtcgtc aaccagcaga cggacgttta
     1801 cgccgctgcg ggctttgcgg gtcagaacct ccaatacgga ttggccggtg gcgtcatgtt
     1861 cgaaaacgta agtcgcgaaa tctatcgact tttccgccga ttcgagcagt tccatgagtt
     1921 tgtgataagt ctcgcagctg tcgctgcata gcgtgatgcg atggccccgt gcgggcaggg
     1981 gcatgccgaa gctgcttata agctcggata accggcgggc ttcatggtct acaagtgcag
     2041 tcgtcatgcc gggaaatgaa atattctgct tgagcgactg cttgtaacgc atctttctgc
     2101 cgccgaaaat aaggtacagc ggcacggcga tgtgaggtat cagaataagg cccatcagcc
     2161 acgccatcgt gcctgacgga ctgcgacgtt gacgaataag atgagaaatg agaatgatgg
     2221 caagaaaaaa ccccag
//
LOCUS       DAONFI010000352         2235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_64329_length_2235_cov_2.832110, whole genome shotgun sequence.
ACCESSION   DAONFI010000352 DAONFI010000000
VERSION     DAONFI010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64329_length_2235_cov_2.832110"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /gene="pstB"
                     /locus_tag="PLK08_09665"
     CDS             <1..166
                     /gene="pstB"
                     /locus_tag="PLK08_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933578.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPS53808.1"
                     /translation="TIVIVTHSMQQAARVSNRTAFFYKGELIEYGLTKLIFTNPADSR
                     TEDYITGRFG"
     gene            206..850
                     /gene="phoU"
                     /locus_tag="PLK08_09670"
     CDS             206..850
                     /gene="phoU"
                     /locus_tag="PLK08_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPS53809.1"
                     /translation="MLELQKKLQSLMLRLDYMGMRVEQQVHDAVESLRKKDADAAKRI
                     IEFDEKVDREEVEIEQECIRLLALYQPAAIDLRTICTIIKVNNDLERIADRAVNLAKQ
                     VKHIIASNIDMEDYKGLLPLEETVFDILNRTMRMINTTEVHTAKEVIEMDDMVDSHYK
                     ELLQMILSRNESAAKAITANNIAKAFERIADLCTNISEDIIFLRTGEIVRHADI"
     gene            1181..2200
                     /locus_tag="PLK08_09675"
     CDS             1181..2200
                     /locus_tag="PLK08_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPS53810.1"
                     /translation="MKMTITKLAKLANVSCGTVSRALNNKAEVNPATKEKVIRLATQL
                     GYEPNGMAKSLRSRQTNALGLIITEIVDPFFAELTQAIEKKADENGFHLVLGVVGNKV
                     RREAEYVRFFQAGRVDGMMIVPASGILGFSELDYIFELKRANFPFVVLGSTNQLETNC
                     VGYDDELGAYKLTSHLIDLGHKKISFIYDISMIGATQPRLNGYRRAFFDHGIAVPEDM
                     LVGVSGTSLNNVGCETARILNRFERPTAIIGINDLVALGILRGIRETGLRVPDDVAVA
                     GFDNIEFLQSLDIPLTTVALPIDELAQKAVDLLITIIHSGSQEKMERIILEPELIIRH
                     STQRK"
BASE COUNT          688 a          468 c          506 g          573 t
ORIGIN      
        1 caccattgta atagtcacac attcaatgca gcaggcggcg agagtaagca atcgcacggc
       61 gttcttttat aaaggtgaac tgatagaata cggcttgacc aaattgatat tcacgaatcc
      121 ggctgactca cgcacggaag actatataac gggccgcttt ggataatcct gaatcacgca
      181 aacgaggata tagaggatat aaaatatgct ggaactgcaa aaaaaacttc agtcgcttat
      241 gctgcggctg gactacatgg gcatgagagt ggaacagcag gtgcacgacg cggtagaatc
      301 tctccggaaa aaagatgctg atgcggcaaa acgtataatc gaattcgatg aaaaagtcga
      361 ccgcgaagaa gtggaaatcg aacaggagtg catacgcctg ctggcgctgt atcagccggc
      421 tgccatcgac ctgagaacca tctgcacgat tataaaggtc aataacgatc tcgaacgaat
      481 agccgaccgt gcggtaaacc tggcaaaaca ggtcaagcac atcattgcaa gtaacataga
      541 catggaggac tacaaaggct tgctccccct tgaagaaacc gtattcgata ttctcaatcg
      601 tacgatgcga atgattaata caacggaagt tcatacggct aaagaagtga tagaaatgga
      661 cgacatggta gacagtcatt ataaggaact gttgcaaatg atattgtcgc gtaatgaatc
      721 agccgcgaag gctataactg ccaacaacat cgccaaagcc tttgaacgca ttgccgacct
      781 gtgtacgaac atttccgagg atattatctt cctgcgaacc ggcgaaatag tacgccacgc
      841 agatatctga tgcatctcaa tatttcgaat gccgccacgc aaaacgattt gtaaattgtt
      901 ctgttacata atgttacggc agtttgccat gcacatatct acaaaaatgg aatattatcc
      961 tatattaggt gatatttctg cattttcacc gagcatttta aactcaaatt gaatttaaaa
     1021 aagtattgac aaaattgatt tgataacata ttatatggtt atggaaacgt tttcggaaaa
     1081 gctgtattat aataatttag cccaatattt attgaattat ccttttaaaa acgtttccat
     1141 aaatggccta tttttacaaa cgtttccgga caaaatttaa atgaaaatga ccatcacaaa
     1201 attagcgaaa ttggcgaatg tatcatgcgg cacagtttcc cgcgcgctga ataacaaggc
     1261 tgaagtcaat ccggctacaa aagagaaagt catccgttta gctacgcagc tgggttatga
     1321 acccaatggc atggcaaaat cactgcgaag tcgtcagaca aatgctctgg ggcttatcat
     1381 taccgaaatc gtagacccgt tttttgcaga attgacgcaa gccatcgaaa aaaaggcaga
     1441 cgaaaatgga tttcatctcg ttcttggcgt agtcggcaat aaggttcggc gtgaagcgga
     1501 atatgtacgt ttcttccagg ctggcagagt tgacggaatg atgattgtgc cggcgagcgg
     1561 aattctcggc ttttccgaac ttgactacat ttttgagtta aagcgagcta attttccttt
     1621 tgtcgtgctg ggcagcacaa atcagcttga aaccaactgc gtcggctatg atgacgaact
     1681 cggtgcatat aaactgacgt cgcatcttat cgatttgggc cataaaaaaa tatccttcat
     1741 ttatgacatt tcgatgattg gagcaactca gccacggtta aatggttata ggcgggcgtt
     1801 tttcgaccac ggcattgcag tgccggaaga tatgcttgtc ggagtaagcg gcacgtcttt
     1861 gaataacgtc ggctgcgaaa cggccaggat tctgaacaga ttcgaacggc ctaccgcaat
     1921 tataggcatt aatgaccttg ttgccctggg cattttacga ggcattcgcg aaaccggctt
     1981 gcgagttccg gatgatgtgg cggtcgcagg ttttgacaat attgaatttc tacaatcgct
     2041 cgatattccg cttacgaccg tagccctgcc gattgatgaa cttgcacaaa aagccgtcga
     2101 ccttctgatt acgattattc attcaggcag tcaggaaaaa atggaacgta tcattcttga
     2161 gccggaactg ataatcaggc actctacgca acgaaagtga ttaggctaat gaatcagctt
     2221 cttattaaaa atcgt
//
LOCUS       DAONFI010000353         2232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_64424_length_2232_cov_4.700046, whole genome shotgun sequence.
ACCESSION   DAONFI010000353 DAONFI010000000
VERSION     DAONFI010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64424_length_2232_cov_4.700046"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PLK08_09680"
     CDS             complement(<1..1377)
                     /locus_tag="PLK08_09680"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPS53811.1"
                     /translation="MSIAEMTTWTDVLQNVFILIYTVLLVVVSVYGLHRYALVFLWWK
                     HKHKAPKPASRFGEMPVVTVQLPMFNERFVAQRIIRETCELDYPKDKLQIQVLDDSID
                     DTTEIAAKAVDEACRQGFDVELIHRTDRTGFKAGALVNGMKTAKGEFIAIFDADFTPK
                     KDFLRNTMDYFTDPRVCVVQTRWEHINRGSSLLTEAQAIFLDGHFAIEHVSRNRSGRF
                     MSFNGTGGIWRRAAIDDAGGWQHDTLTEDLDLSYRAQMKGWKFVFLPNETSPAELPPE
                     MMAFKAQQFRWTKGGTQSCIKLLPLVMKSNLPLKVKAECFFHLTGFCLHIFMFLLVLL
                     MFPAMFSQTLPLKTGTFIRGLADIGIFVLATMSGSVFYCMGQWALSGKWYKPVIYFPY
                     MVALGVGLCVSNTKAILEAIAGKESVFVRTPKYGSASMKEYSDKTEEKSCKNKSSSQM
                     RLMVVEFIM"
     gene            complement(1486..1992)
                     /locus_tag="PLK08_09685"
     CDS             complement(1486..1992)
                     /locus_tag="PLK08_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPS53812.1"
                     /translation="MPESTIVIVKTNRGDMEIELDDVKAPKTVDNFLTYVDEGFFDGT
                     IFHRVIPGFMIQGGGFTSEMEQKPTNAPIQNEATNGLKNERGTIAMARTPNPHSASSQ
                     FFINLVDNDFLNFTGQNMQGWGYCVFGRIVKGMDVADEIAKVRTGNHGMHGDVPVETV
                     EIISIKRK"
     gene            complement(2052..>2232)
                     /locus_tag="PLK08_09690"
     CDS             complement(2052..>2232)
                     /locus_tag="PLK08_09690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPS53813.1"
                     /translation="NVLQNNGRIAGQLVPHVHFHIIPRYGAAGEFRFDWPAGKYQDGK
                     MQEFADKIKSHLSGK"
BASE COUNT          590 a          589 c          471 g          582 t
ORIGIN      
        1 catgatgaat tcaacaacca tcagccgcat ttgcgacgac gatttatttt tgcatgattt
       61 ttcttccgtt ttgtctgaat attccttcat ggaagcactg ccgtatttcg gcgtacgaac
      121 gaacacgctc tccttgccgg caatcgcctc aagaatcgcc ttcgtattcg acacgcacag
      181 ccctacgcca agagcaacca tatatggaaa atagattact ggtttatacc atttgccact
      241 aagagcccac tggcccatgc aataaaaaac cgaaccggac atggtcgcga gcacaaatat
      301 gcctatatct gccaagccgc gaatgaacgt acccgttttg agcggcagag tctggctgaa
      361 catggccgga aacatcagca acaccaacag aaacatgaag atgtgcagac agaaaccggt
      421 caggtgaaaa aagcattccg ctttaacttt cagcggcaga ttacttttca tcacaagcgg
      481 caggagtttt atgcaacttt gagtgccgcc cttcgtccag cggaactgct gagccttgaa
      541 tgccatcatc tccggcggaa gctcagccgg actcgtctcg ttcggcagga agacaaactt
      601 ccagcccttc atctgggcac gataactcaa gtcaaggtct tccgtaagag tatcatgctg
      661 ccagccgccg gcatcgtcta tagcagcacg acgccatatg ccacccgtgc cgttaaaact
      721 cataaaacgg ccactgcgat tgcggcttac atgctcaatg gcaaagtggc cgtcaaggaa
      781 aatggcttgc gcctcggtaa gcaggctgct gccgcggttg atatgctccc agcgggtctg
      841 cacaacacaa actctggggt cagtgaaata atccatcgta ttacgtaaaa aatctttctt
      901 gggagtaaag tcggcatcaa aaatcgcgat aaattcgcct ttcgccgtct tcatgccgtt
      961 cacaagagca ccggctttga aacctgtccg gtcagtgcga tggataagtt ccacatcaaa
     1021 accctgacgg caagcctcgt cgacggcttt tgcggcgatt tccgtcgtat catctatgct
     1081 atcgtccagc acctgtatct gaagtttgtc cttcggataa tccaactcgc acgtttcgcg
     1141 tattattcgt tgtgccacaa aacgctcatt aaacatcgga agctgaacgg tcaccaccgg
     1201 catttcgccg aagcgtgatg ccggcttggg agctttgtgc ttgtgtttcc accagagaaa
     1261 aacaagcgcg tatcgatgaa gcccatatac ggacaccacc accagcaata ccgtataaat
     1321 caatataaac acgttttgaa gaacatcagt ccaggttgtc atttccgcaa tagacatttt
     1381 attccttcta accgtatccg gaaaggtccg gattcttttt caaaaacctg aaccgccgat
     1441 ttcaaagccc ccatattcag gaaatcggcg aatcggacaa ttccattact tacgtttgat
     1501 agagattatt tctacggtct caaccggcac atcgccatgc attccatgat tgccggttcg
     1561 cacctttgca atttcatccg ccacatccat gcctttgact atcctgccga aaacgcagta
     1621 tccccagccc tgcatattct ggccggtaaa attcaaaaaa tcattatcaa caagattgat
     1681 gaaaaactgg ctcgatgcgc tatgaggatt gggggtacga gccattgcta ttgtgccgcg
     1741 ttcattttta aggccgttag tcgcctcgtt ttgtatcgga gcattggtgg gtttctgttc
     1801 catttcagac gtaaacccgc cgccctgtat catgaaacca ggaatgacgc gatgaaaaat
     1861 cgttccgtca aaaaaacctt catccacata agttaaaaaa ttatccacgg ttttcggcgc
     1921 tttgacgtca tcgagttcga tttccatatc accgcgattt gtttttacta ttacgattgt
     1981 cgattccggc ataaaaaatc cttattttag tattttgtaa ttttaacctt tttgccgtca
     2041 gccggcagta attatttccc cgaaagatgg cttttaattt tatcggcaaa ttcctgcatc
     2101 ttgccatcct gatatttgcc cgccggccaa tcgaagcgga actcaccggc tgcaccatat
     2161 cgcggaataa tgtgaaaatg cacatgcggc acaagctgtc ctgctatacg gccattgttt
     2221 tgaagcacat tg
//
LOCUS       DAONFI010000354         2232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_64426_length_2232_cov_3.954525, whole genome shotgun sequence.
ACCESSION   DAONFI010000354 DAONFI010000000
VERSION     DAONFI010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64426_length_2232_cov_3.954525"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLK08_09695"
     CDS             <1..1197
                     /locus_tag="PLK08_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPS53814.1"
                     /translation="SEEEFEDLEKIAGESPVIRYVNYVISSAVGEGASDIHIEPGEKT
                     LKVRFRMDGILFDQPSPPLSMHAAIISRLKIMANLDIAERRLPQDGRIRATVKRRNID
                     LRVSILPTVHGEKCVIRLLDNRSISVGLENLGFSSETLTLFKNQILQPHGVVLVTGPT
                     GSGKSTTLYSALQIMDRERMNISTVEDPVEYELGGINQVNVIDSIGMSFAAALRSLLR
                     QDPDVIMVGEIRDPETARIAVQAALTGHLVLSTLHTNDAPSSVTRLINIGVEPFLISA
                     SVNAILAQRLVRRICPNCKVEVPQNNVREGEYLRRNGIEDMTLFRGEGCEQCRHTGYK
                     GRVGIYELLVIDDVIRTMIPQNPSVTELTKLAIEHGMISLKMDGLNKVAKGLTTVEEI
                     MRVTES"
     gene            1220..>2232
                     /locus_tag="PLK08_09700"
     CDS             1220..>2232
                     /locus_tag="PLK08_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPS53815.1"
                     /translation="MEIHELLRTALTSSASDVHLVPGQPPIMRVHTMLEPIPGYDVFT
                     KDVAEELIKTLVEPARMERLKNHLDLDFGLESPGNGRFRVNAHYQKNGLGFAFRAIPQ
                     KVPALADLNLPAGVQEFVKYPRGLVLVTGATGSGKSTTLASMINAMNQSINRHIITLE
                     DPIEYELVSKTCLIEQREVGQDVESFASGLKHALRQDPDVILVGEMRDLETTSAAITA
                     AETGHLVLSTLHTQSAHQTLARIIDIYPPAQQNQIRSMLATTLRAVVTQTLFRRHDIK
                     GMAPACEIMVCTSAIANCIRENRLHEIPNIIETSRQQGMQLLDDSIKGLVLNGLISPK
                     EALT"
BASE COUNT          592 a          604 c          581 g          455 t
ORIGIN      
        1 agcgaagaag aatttgaaga cctcgaaaaa atcgccggcg aaagtcctgt catacgatac
       61 gtcaactacg tcatatcgtc cgccgtcggc gaaggcgcca gcgacattca catcgaacca
      121 ggcgaaaaga cattgaaagt ccgattccgc atggacggca tactgttcga tcagccgtcg
      181 ccgccgctga gcatgcatgc agcaatcatt tcgcgtctga aaatcatggc gaacctcgac
      241 atcgccgaac gccgcctgcc gcaggacggc agaatccgtg ctacggtcaa acgtcgcaat
      301 atcgaccttc gcgtaagcat actgccgacg gtacacggcg aaaaatgcgt cattcgtctg
      361 ctcgataatc gttcgataag cgtaggcctg gaaaatctgg gattttcttc ggaaacgctg
      421 acgctcttta aaaaccagat acttcagccg cacggcgttg tgcttgtaac gggaccgacc
      481 ggcagcggca aaagtacgac gctctattcc gcgcttcaga taatggacag ggaacggatg
      541 aacatctcca ccgttgaaga cccggtggaa tatgaactcg gcgggataaa ccaggttaac
      601 gtcatcgact cgataggcat gtcgtttgcc gccgcgctta gaagccttct gcgacaggac
      661 cctgatgtta taatggtcgg cgaaattcgt gaccctgaaa cggcccgcat agcggtgcag
      721 gccgcgttga ccggccacct ggtgctttcg actctccata ccaacgacgc accttcaagc
      781 gtgacgcgac tgataaacat cggcgttgag ccgtttctga tttcggcatc ggtcaacgcc
      841 attctggcac agcggcttgt gcgaagaatc tgcccgaact gcaaggtcga agtgcctcag
      901 aacaacgtgc gggaaggtga atatctgcgt cgaaacggca tagaggacat gacgctcttt
      961 cgcggcgaag gatgcgagca atgcaggcat acgggttata aaggtagggt cggcatttac
     1021 gagcttctgg ttattgacga tgttatacgc acgatgatac cgcagaatcc atccgtgacc
     1081 gaattgacca aactggccat tgagcacggc atgataagcc ttaaaatgga cggccttaac
     1141 aaggtcgcca aaggccttac gactgttgaa gaaataatgc gagttaccga atcctgatac
     1201 gtcggagatt ttctgtcaaa tggaaataca tgaacttctt agaactgcgt tgacgtctag
     1261 tgcgagcgac gtgcatctag tccctggcca gccgcctata atgcgcgtac ataccatgct
     1321 cgaaccgata ccgggttatg acgtatttac aaaagacgtt gctgaagaac tgattaaaac
     1381 tctcgtcgag ccggccagaa tggaacgtct gaaaaatcat ctcgacctcg atttcggcct
     1441 tgagtcgccc ggcaatggcc gttttcgcgt caacgcccat taccagaaaa acggcttggg
     1501 tttcgcattt cgtgccattc cgcaaaaggt gccggcgctg gcagacctga acctgccggc
     1561 cggcgtgcag gaattcgtca aatatcccag gggtctggta ctcgtaacgg gcgcgacggg
     1621 aagcggcaaa agtacgactc tggcgtcaat gataaacgcc atgaaccagt ccatcaacag
     1681 gcatatcata acgctggaag acccgataga atatgaactg gtaagcaaaa catgtcttat
     1741 cgagcagcgt gaagtcggcc aggacgtgga aagtttcgca agcgggctca aacatgccct
     1801 gcgtcaggat ccggacgtca ttctcgtcgg tgaaatgcgc gaccttgaaa ccacgtcagc
     1861 cgccataacc gccgccgaaa ccggccacct ggtgctttcg accctgcata cgcaaagtgc
     1921 gcatcagacg ctggcacgta taatcgacat ctatccgccg gcacagcaga atcagatacg
     1981 cagcatgctg gcaacgactc tgcgggcagt cgttacgcag acgcttttca gacgtcacga
     2041 cataaaaggc atggcgccag cctgcgaaat aatggtatgt acgtcggcaa tagcgaattg
     2101 cattcgtgaa aaccgcctgc atgaaattcc gaacatcatt gaaacaagcc gccaacaggg
     2161 catgcaactg ctggatgatt ccatcaaggg cctcgtactc aacggcctca tttcacccaa
     2221 agaagcactt ac
//
LOCUS       DAONFI010000355         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_65661_length_2204_cov_4.140065, whole genome shotgun sequence.
ACCESSION   DAONFI010000355 DAONFI010000000
VERSION     DAONFI010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65661_length_2204_cov_4.140065"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLK08_09705"
     CDS             complement(<1..366)
                     /locus_tag="PLK08_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPS53816.1"
                     /translation="MEKLKIDGFDVVVRRCETLIVGAGAAAMNCAVHLAEFNRTNGKA
                     DFAAGVKIITGGIPLGTSRMSGSDKQTYYKLGTSAETADSAMSMAKSLTAAGCCHEDL
                     ALIESITSLREFFHLVRSGV"
     gene            complement(368..1144)
                     /locus_tag="PLK08_09710"
     CDS             complement(368..1144)
                     /locus_tag="PLK08_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HPS53817.1"
                     /translation="MSDKKVAVVTGSGRGIGMAIAVQLAGQGYDIVVADVCSPEDAAE
                     AIAEIQKAGSKGFFCKCDISDSRSRQELRDFTREKFGCCNLLVNNAGVAPITRMDILE
                     TTQESVERLLRINLEGTFFMSQLFANWMIEQKNAKPDGEFRIVNISSMSAYTSSTSRG
                     EYCISKAGISMVTKLFADRLAEFGIGVFEIRPGIIETKMTSGVKEKYDKLIAEGITPI
                     KRWGKPQDIAKAIGAIAAGNLDFSTGQVINVDGGFHLRRL"
     gene            complement(1775..2110)
                     /locus_tag="PLK08_09715"
                     /pseudo
     CDS             complement(1775..2110)
                     /locus_tag="PLK08_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin oxidoreductase/NADH oxidase"
BASE COUNT          460 a          623 c          547 g          574 t
ORIGIN      
        1 cacgccgctg cgcaccagat gaaagaactc ccgcaaagac gttattgatt ctataagcgc
       61 caggtcttcg tggcagcagc cggctgcagt gagactttta gccatggaca ttgcgctgtc
      121 agccgtttcg gctgacgtgc ccagcttgta ataagtctgc ttgtccgagc cgctcatccg
      181 gcttgtgccg agcggtatgc cgccggtaat gatttttacg ccggcggcga aatcggcttt
      241 gccgttggtt ctgttgaatt ccgcaagatg cacggcgcaa ttcatcgccg cggcgccggc
      301 tccgacgatg agtgtttcac agcgtcgcac gacgacatca aaaccgtcta tctttaattt
      361 ttccatatta cagcctccgc aggtggaatc cgccgtctac gtttattacc tggccggtcg
      421 agaaatccag attgccggcc gctattgcgc caattgcctt ggcgatgtcc tgaggtttgc
      481 cccaacgctt tatcggcgtt atgccttcgg cgatgagctt gtcatatttt tcctttacgc
      541 cggacgtcat cttcgtctcg ataatgccgg gccgtatctc aaaaacgcct atgccgaatt
      601 ccgcgagccg gtcggcaaag agtttcgtga ccatggaaat gccagctttg cttatgcaat
      661 attcgccacg cgacgtggac gacgtatagg ctgacatcga gcttatgttc actatgcgaa
      721 attcgccgtc aggtttcgca tttttctgct ctatcatcca attcgcaaaa agttgcgaca
      781 tgaaaaacgt gccttcaaga ttgattcgca acagccgctc gacactttcc tgcgtcgttt
      841 cgaggatatc cattcgcgtt atcggcgcaa cgccggcatt gttgaccagc agattgcagc
      901 agccgaattt ttcgcgtgtg aaatcgcgca gttcctgccg cgaacggctg tctgaaatat
      961 cgcatttgca aaaaaagcct ttgctgccgg ctttttgaat ttcggctatc gcttcggcag
     1021 cgtcttcagg gctgcaaaca tccgcgacga cgatatcata accctggccg gcaagttgga
     1081 cggctatcgc catgcctatg cctctgcctg aacccgtaac tacagccact tttttgtcac
     1141 tcattttttt atctccattc tgtttagcct tttgccggcc ctgccggaaa ataggcccgt
     1201 aaatttccga atctcttatc gggcagcctg ccatgccgcc gtcgcgcatt atctgcgtac
     1261 aactgctgca cgcgatgcaa actttattcg ggtcgagttc gcctttggca attatgtccg
     1321 cggcaaaatc gggatatgca aacgccagcc ggccgacgcc gactattttg gccaatttcc
     1381 gttcgataac tccggctgcc acctgcggca tgaactgccg cagccaggaa taccccgtcc
     1441 cgattatcgt catgtcagga tgtttttgct gaatttcgcc ggccaggttt atcagccgtg
     1501 ccactcctgc aagcggatgc tccggccgcg gctggccgcc ggcggtgggc ttttcaaacg
     1561 gccgattata aaacggatta taataaggat tcgccagcgt gacgtttatc atgtttatgc
     1621 cggcgtcttc gtgtaattgc gtcacaagcc tgtccggctc ggcaaggtca gccttcaggt
     1681 aatcatcttt gcaaactccc cagccgtgag gatattcgat ggcatcaaaa agtcccagcc
     1741 gtgtcgtaat tgatttttcc ttgccgaacc gtttgcggat tttagccgtc acttcacgca
     1801 acatgcgagt gcgattttca aaactgccgc cgtatcggcc ttcccgcaga tggccgctca
     1861 ggagttcatg cagcagatag ccgtgacagc attttacgtc cacagcgtca aaccccacag
     1921 aatacgccag ctcggcagcc tccagaaaat gagcctgcaa ttcgtccagg taatcatcgc
     1981 tgacgacagg ataatcgtgc ggcagtttaa ttgccgcgtc gcgattgggg tcgtgatgag
     2041 ctataatcgg ctccggtttt ccagacggtt ttgaataacg gcctgaatgc gtcagttgcg
     2101 caactatcat aagccggtgt tcaccgccaa accgttctgc cgccgttttg cgcatcatat
     2161 cgacaagttc gcaaaatgaa tccgcgttgt tgcggtttat gtgg
//
LOCUS       DAONFI010000356         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_65963_length_2198_cov_4.342977, whole genome shotgun sequence.
ACCESSION   DAONFI010000356 DAONFI010000000
VERSION     DAONFI010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65963_length_2198_cov_4.342977"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1252)
                     /gene="ilvE"
                     /locus_tag="PLK08_09720"
     CDS             complement(377..1252)
                     /gene="ilvE"
                     /locus_tag="PLK08_09720"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HPS53818.1"
                     /translation="MKVWLDGKLVDEADAKISVFDHGLLYGDGVFEGIRAYHGSIFQC
                     QAHLDRLFESAEIISLDMPYTRQQLRDATYETLRANKLDDSYIRMVITRGKGTLGLNP
                     KKCSEASAFIITGNIKMYPEEMYRNGMPVIIGKTIRTSVTMFNPAVKSLNYLNNIMAK
                     LEGIAAGVEEVLMTNDKGNIAEASGDNVFIIKDGVLMTPPKEAGILLGVTRSVVMHMA
                     RKLGMKVLEINITPKDVYQADECFLTGTAAEVISVTSVDGKIIGCGKAGAVTQKIRAA
                     FKEFVKTDEQIPYIG"
     gene            1473..>2198
                     /locus_tag="PLK08_09725"
     CDS             1473..>2198
                     /locus_tag="PLK08_09725"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPS53819.1"
                     /translation="MKLGELAELIGGRVDGDASVEITGLTTLTLAGSSDVSFLTNIRY
                     QHQLAETKAAAVIVGDDQAAVPGLTLVHCKDPYFSFRQAMVKIYGFRRHPFSGVSSQA
                     SVSSSAKLGKNVTVAPFAVVCDNVVVGDDTVLYPGVFVGPRCRIGDGCVLYPNVVVYD
                     DCILGNRVTLHAGCAVGVDGFGYSTHKCEDGVVRHDKIPPAGNVVIEDDVELGACCVI
                     QRATIGSSTIGAGTKFADLIGIGH"
BASE COUNT          547 a          534 c          533 g          584 t
ORIGIN      
        1 cgcaaaattg ctcgatttcg gcgacgccca tgtttttggg ataggttcta cttattgtac
       61 tgttttcgta cgcttcaaga acataaatga tataagtgaa tgtctaagga ttaaaatcgt
      121 aacaaaatta agaaagccat agtaaaagta gccttttatt gaagtatccc acaaaatttt
      181 gaaaaagaaa aaccagagcc tcacaaaaat acgactattt tttgaaatgg cggccgccaa
      241 ccgttaattc aacaaaccag ccctaccggc atatcaaact ccagcctccc ataacaacgt
      301 cagatttgcc caatactaaa tataggatat tcggcagcga taagaatctt taaaaaaaat
      361 gcgtaatctg tttttatcag cctatatacg gtatctgctc gtcggttttg acgaattctt
      421 taaacgcggc gcgtattttt tgggtaacag cgccagcctt gccgcagccg atgattttgc
      481 cgtccacgct ggtgacggat atgacttccg ccgccgtgcc ggtcaagaaa cattcgtcag
      541 cctgataaac atctttcggc gttatgttta tctccagcac tttcatgccg agttttctcg
      601 ccatatgcat caccaccgag cgcgtcacgc caagcagtat gccagcctct ttcggcggcg
      661 tcatgagcac gccgtccttg attatgaaca cgttgtcgcc ggaagcctcg gcgatattcc
      721 ccttgtcatt tgtcatgagt acttcctcga cgccggctgc gattccttcg agtttagcca
      781 ttatattgtt gaggtagtta agactcttga cggctggatt gaacatggtc acgctcgtcc
      841 gtatggtttt gccgataatc accggcatgc cgttacggta catttcttcg ggatacattt
      901 ttatattgcc ggtgatgatg aatgccgacg cttctgagca ttttttgggg ttaaggccga
      961 gcgttccctt gccgcgagtg ataaccatgc gaatatacga gtcgtcaagt ttattggctc
     1021 tgagcgtttc atatgtggcg tcacgaagct gctgacgggt atagggcatg tccagagata
     1081 taatttccgc cgactcgaaa agcctgtcca gatgagcctg gcactggaaa atactgccgt
     1141 gatacgcgcg aatgccttca aacacaccat cgccgtaaag caggccatgg tcaaaaaccg
     1201 atattttcgc atccgcctca tcaaccagtt taccgtccag ccaaactttc atcgcaaaac
     1261 tcctgaatgg gaacattgaa tacctggatt ttaccgtaac cgttcaatat atccattcag
     1321 gcgattactt taaagttttt tccgtagtta atagccaaaa aacctttaag tcggcggttt
     1381 atttgagtaa tctagtatcc tatggcagta tttgggtaat tgtttccagt aatgggatta
     1441 taaaagcata gctgccaaat ggagttattc ggatgaaact aggtgaattg gcggaactta
     1501 tcggcggccg ggtggacggc gatgcctcgg tagaaataac cggcctgacc acattaacac
     1561 ttgccggcag cagcgacgtg agttttttga ccaacatacg ctatcagcat caattggctg
     1621 agacgaaggc tgcggcggtg atagtcggcg atgaccaggc cgccgttccc ggcctgactc
     1681 tggtacattg caaagaccca tatttttcat tccggcaggc gatggtgaaa atttacggtt
     1741 tccggcggca tccgttcagc ggcgtttcgt cgcaggctag tgtgtcaagt tcggcaaaac
     1801 tcggcaaaaa cgtcactgtc gcgccttttg cggttgtttg cgataatgtt gtcgtcggcg
     1861 acgacaccgt gctgtacccg ggggttttcg taggcccccg atgcaggata ggcgacggct
     1921 gtgtgcttta tccaaatgtt gtcgtctatg atgattgcat cctgggcaat cgcgtcacgt
     1981 tgcacgccgg ctgtgccgtc ggcgtcgacg gattcggtta ctcaacgcac aaatgcgaag
     2041 acggcgtggt acgacacgac aaaataccgc cagccggcaa cgtcgttatc gaagacgacg
     2101 tcgaactggg cgcatgctgt gtaattcaga gagccaccat cggttcgagc accatagggg
     2161 ccggtacgaa atttgcggat ttgataggca tcggccat
//
LOCUS       DAONFI010000357         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_65971_length_2198_cov_3.690154, whole genome shotgun sequence.
ACCESSION   DAONFI010000357 DAONFI010000000
VERSION     DAONFI010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65971_length_2198_cov_3.690154"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLK08_09730"
     CDS             <1..193
                     /locus_tag="PLK08_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53820.1"
                     /translation="AICFAFLASQSTNYASVRYLILLEALKAVSGNHASAASQKLSQL
                     TNSLSFLFITSSQKTTGLT"
     gene            complement(390..1472)
                     /gene="recA"
                     /locus_tag="PLK08_09735"
     CDS             complement(390..1472)
                     /gene="recA"
                     /locus_tag="PLK08_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPS53821.1"
                     /translation="MARKTATKAEEVSESDKNPQRSAALDAALKQIEKNYGVGSIMTL
                     SNASAVQIPGISTGAISLDLALGGNGVPQGRICEMYGPEGSGKTTLALHVIASAQKTG
                     GVAAFIDAEHALDPTWARKIGVKLDSLLVSQPDNGEQALEICEMLVRSNSVDVVVVDS
                     VAALIPKAEIEGEMGDSHVGLQARLMSQAMRKLVGAVAKSRTTVIFINQIRMKIGVMF
                     GNPETTTGGNALKFYSSVRLDVRRISTLKNGDDPIGNRVRVRVVKNKIAPPFKSAEFD
                     IMFDSGISFIGDLLDLAVAQDIIQKQGAWFSYGDVRLGQGRENSKKFLAENPDLVEEV
                     KQKIMQAVQNAGEMKEVGEVTASGDE"
     gene            complement(1587..>2198)
                     /locus_tag="PLK08_09740"
     CDS             complement(1587..>2198)
                     /locus_tag="PLK08_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPS53822.1"
                     /translation="ELNVLIEAAPAIPENFRDSEYVFLANTHPALQMELLSQCPAAKL
                     VVADTMDLYINTEKTALTELLKKIDGVIVNDSEALLYTGRKNIIEAGLEISRLCKRFC
                     VIKKGEHGCILFIGGCAYPMPGYPASKVVDPTGAGDSFAGGMMGYISQQDKVDDATLR
                     RAMAYGTVVASLELEDFSLNRLRKITRNEIDDRFSGFAKITQF"
BASE COUNT          514 a          601 c          473 g          610 t
ORIGIN      
        1 ggctatctgc ttcgccttcc ttgccagcca atcaaccaac tacgcctcgg tgcgatatct
       61 cattttgtta gaagctctaa aggctgtgag tggtaatcat gcatctgcgg cgtcacaaaa
      121 actcagccaa ttgacaaatt cgctgtcgtt tttgtttatt acatcctcac agaaaacaac
      181 gggcttaact taagggtatt tttataaaac ccatccctaa aaccttggct gcctgcaatc
      241 ggctcttttg caacctggcg gcatcagaaa aacggcagaa tattttttaa aaaaaatatg
      301 ccgccacttt ttcttccttg ccaatccgca aaattactcg atttcagcga cacccatgtt
      361 tgggggacag gttctataac cttttcacgc tattcatcgc cgctggcggt gacttcacct
      421 acttctttca tctcgcccgc attctgaacg gcttgcatga ttttctgttt tacttcttca
      481 acgaggtcgg gattttccgc taaaaacttc ttggaatttt ccctgccctg gccgagccgg
      541 acatcgccat agctgaacca tgcaccctgt ttctggataa tatcctgagc gacggctaaa
      601 tcgagcaggt cgccgataaa actgatgccg ctatcgaaca taatgtcaaa ctcagccgac
      661 ttgaacggcg gtgcgatctt gttcttaaca acgcgaactc gaacgcggtt gcctatcggg
      721 tcatcgccat tcttgagcgt cgaaatgcga cgtacgtcaa gccgcaccga cgaataaaat
      781 ttcaaggcgt taccgccggt cgtcgtctcc ggattgccga acatcacgcc tattttcata
      841 cgaatctggt ttataaaaat aacggtagtc cggctttttg ccactgcacc gaccagttta
      901 cgcatggctt ggctcataag ccttgcctgt aaacccacgt gcgaatcgcc catttcgccc
      961 tcgatttcag ccttgggtat cagtgccgca accgagtcga ccaccacaac gtctaccgaa
     1021 ttactacgaa cgagcatttc gcatatttcc aacgcctgtt cgccgttatc cggctggctg
     1081 acaagcagcg aatccaattt tacgccaatt ttacgcgccc aagtggggtc aagcgcgtgt
     1141 tccgcgtcaa tgaacgccgc cactccaccg gttttctgtg cactggcaat gacatgtaac
     1201 gccagagtcg tcttgccaga accttcaggg ccatacattt cgcagattct gccctgcggt
     1261 actccattac cgcccaatgc caggtcaagg ctgatggcac ccgtgcttat accaggtatc
     1321 tgtactgcac tggcgttgga cagcgtcatt atactgccga cgccgtaatt cttttctatc
     1381 tgtttgagag ccgcatccaa agcagctgac cgctggggat ttttgtcgct ttccgatacc
     1441 tcttctgcct ttgtcgccgt cttccgtgcc atttttatat tcctttgttt atgttatgtt
     1501 cggatgaagt gtaagctgaa tgttagtaat ttgcaagaat attttccgaa caattcccaa
     1561 atttttttat ccgcagtttc aatgcctcaa aactgcgtta ttttcgcaaa accgctgaaa
     1621 cggtcgtcaa tttcgttgcg ggttatttta cgaagtctgt tcagtgagaa atcctctagt
     1681 tccaggctag ccacgacagt accgtaggcc atcgcccgtc gaagggtcgc atcatcgact
     1741 ttgtcctgct gcgaaatata acccatcatg ccgccggcaa aactgtcgcc tgcgccggtt
     1801 gggtctacaa ccttcgatgc cggatagcca ggcatcggat acgcacagcc gccgatgaac
     1861 aatatgcacc cgtgctcgcc cttctttatg acgcagaaac gcttgcacag acgcgatatt
     1921 tccagaccag cctctattat gtttttcctg ccggtgtaga gcaacgcctc agaatcgttc
     1981 actattacgc cgtctatttt tttcaacaat tcagtaaggg cagttttttc ggtattgatg
     2041 tacaaatcca tcgtatcggc aacaacaagt ttggcggctg gacattggct caaaagctcc
     2101 atctgaagtg ccggatgggt gttagccagg aaaacatatt cactatcgcg gaaattttcc
     2161 ggaatggctg gagcggcttc gataagcacg ttcagttc
//
LOCUS       DAONFI010000358         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_66385_length_2189_cov_3.813964, whole genome shotgun sequence.
ACCESSION   DAONFI010000358 DAONFI010000000
VERSION     DAONFI010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66385_length_2189_cov_3.813964"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLK08_09745"
     CDS             <1..482
                     /locus_tag="PLK08_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53823.1"
                     /translation="PSKNPTRYFTSLLYPFEGDKRPDVSFKDGVVKIGNDRVDNYFRK
                     GEQTDGDCALVSRQKEKLKHVLLVRGSFIKDVIHSDGSVQFLIINRTDDATLDIKVIG
                     GDKATRLNLTGFDGIKQATVNGQAANLAYDKNMLIINGPFMKVTPDPNNTKLFWIVK"
     gene            756..829
                     /locus_tag="PLK08_09750"
     tRNA            756..829
                     /locus_tag="PLK08_09750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:790..792,aa:Val,seq:gac)
     gene            1288..>2189
                     /gene="groEL"
                     /locus_tag="PLK08_09755"
     CDS             1288..>2189
                     /gene="groEL"
                     /locus_tag="PLK08_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPS53824.1"
                     /translation="MAAKQLAFDTEVREKLLTGVQKLARAVKSTLGPRGRCAILDKGW
                     GAPTVTKDGVTVAEEITLKDKNENLGAQLVKEAASKTSDAAGDGTTTATVLAEAIFSE
                     GYRNLAAGADAMALSRGIAKAVDAITAELAKMSIPVSGDKAKIRNIAAVSANNDAVIG
                     KMLAEAMEKVGQDGVITVEEGKGIETSIEVVEGMQFDRGYLSPHFVTDQESMECVYEK
                     PLILIYEDKISNIQALVPLLEKVAKTSRPLMIISEDVEGEALATLVVNKLRGVLNVVA
                     VKAPGYGDRRKAMLQDIAILTGGEV"
BASE COUNT          599 a          467 c          548 g          575 t
ORIGIN      
        1 ttccgagcaa aaatccgacg agatatttta cgtcattgtt atatccgttt gaaggggata
       61 aacgcccgga cgtgtccttt aaggacggcg tagtcaaaat cggcaatgac agggtggata
      121 attacttccg caaaggtgaa caaaccgatg gcgactgcgc ccttgtcagc cgtcaaaagg
      181 aaaagttaaa acacgtctta ctcgtacgcg gcagttttat caaagacgta atacatagcg
      241 atggcagtgt ccaatttctt ataatcaatc gtaccgatga tgcgacattg gatataaagg
      301 ttattggcgg cgataaggca accaggctga atttgactgg ttttgatgga ataaaacagg
      361 caaccgttaa cggccaagct gcaaatctgg catatgacaa gaatatgctt attattaacg
      421 ggccgttcat gaaggtaacg cctgacccga ataacaccaa actgttctgg atagtgaaat
      481 agaacattag tatttccgta tatctgtcgc agaaaatcag gaaatataag gtataaccga
      541 gagacttcca acagtggctg actttgtgag cggcaaagac agccactgtt ggttatttaa
      601 ggtgtggttt gtgaatgtct ttgtcaaact gtatggagat gttttttatg gtttatccgc
      661 cgacgcatca aaaatgaaaa aacatatccg actctttttt atgaaagttt gcttgactat
      721 cggcagggca gaatatatat tgacgcccca cacatgggct gttagctcag atggctagag
      781 cgcctgctcg acacgcagga ggtcgttggt tcgagtccaa tacggcccag ttgtttttaa
      841 aggcatcatt tttgttgcga atctttgaat tattaattat tcatgcctgc tttaatttct
      901 gttaatcata tggcttatct gttccatccc gtatcttggg tagtttctta gctttgcctg
      961 ctattatttt gagaactgct agaaaatgcc aatataagcc cacctctaat tgactgtcaa
     1021 tttttgcttt atgtatattc aacctgtcat ctgtgcaggc tttgttttgc cgtaacaaat
     1081 ctatttgtat ttaacatctt gttgaatata tagttatata gatatcgttt tttggcacgt
     1141 tagttgcatg cgtttattga tggttgccgt gcgcagaaag tgcaggcact ccataaacgg
     1201 cggctcgtgg ccgtctgcat ggcaattaca ccaacaagtg ttgttcaaca attataaaat
     1261 tcttttattt gtcgaaagga gatgtcgatg gcagctaaac aactggcatt tgacacggaa
     1321 gttcgcgaaa agcttttgac aggcgttcag aagctggctc gcgcggtaaa aagtactctt
     1381 ggaccccgcg gcagatgcgc gattcttgac aagggctggg gtgcgccgac tgtgaccaaa
     1441 gacggcgtaa ccgtggcgga agaaattacg ctgaaagata agaacgaaaa tcttggtgcg
     1501 caacttgtga aagaagccgc cagcaaaaca tccgatgccg ccggcgatgg cacaacgacg
     1561 gctacggtac ttgccgaagc gattttctcg gaaggatatc gaaaccttgc ggctggcgcc
     1621 gacgccatgg cattgtcgcg cggcattgca aaggctgtcg atgcaatcac ggctgaactg
     1681 gcgaaaatgt cgattccggt ttccggcgac aaggctaaaa ttcgcaacat agcagctgta
     1741 tccgcaaata acgatgccgt catcggcaaa atgctggctg aagccatgga aaaagtcggc
     1801 caggatggcg ttataacggt tgaggaaggc aagggcattg aaacctccat agaagtcgtg
     1861 gagggcatgc agttcgaccg cggttatctc agcccgcatt tcgtgaccga ccaggaaagc
     1921 atggaatgtg tctatgaaaa accgctgatt ctcatctacg aggacaaaat ttccaatatt
     1981 caggctctcg tgccgctact ggagaaggtt gccaagacca gccggccgct gatgataatc
     2041 tccgaagacg tcgaaggcga agcgttggct acgctggttg tcaacaagct gcgcggcgta
     2101 ttgaacgttg ttgccgtgaa agcacctggt tatggcgacc gtcgcaaggc aatgctccag
     2161 gatattgcca tactcaccgg cggtgaagt
//
LOCUS       DAONFI010000359         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_67276_length_2170_cov_4.245863, whole genome shotgun sequence.
ACCESSION   DAONFI010000359 DAONFI010000000
VERSION     DAONFI010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67276_length_2170_cov_4.245863"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLK08_09760"
     CDS             complement(<1..783)
                     /locus_tag="PLK08_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53825.1"
                     /translation="MKMYTQARKTNTGLYVTILALAGVLLAVVFWPSGRKPAENPPAL
                     EPAPQPGYPKISGPEEFSTADKTQQKNDTAQEHAQKSQPVTAYSEKSAAKDTDKAYGP
                     KGMTVALSRAIGPSPLTTPRINADAARQAYAQGWVLYQQNKDYIQARTLLNRALNSGF
                     LAEPQAVIARKALEDLANRTVLNPSPYVNPKDPYLISYKFKSGDTLNSIRGAGGKITR
                     EGIISHYDLCVPARMIPYVNGFGLSARNFQAGRNYKLVRGPFH"
     gene            complement(885..1445)
                     /locus_tag="PLK08_09765"
     CDS             complement(885..1445)
                     /locus_tag="PLK08_09765"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPS53826.1"
                     /translation="MRVMMLNDQKRKIRSDVRAALAKLCEPELQCKSDVICRRLADTP
                     GFLQAACIMLYLPIDGEVRLGGLARQAWRAGKTVCAPTSPLDREMRAIEIRPENMSLF
                     SPEMGLRSPLGREIFIDEIDFIVVPGLAFDRNGGRLGRGGGFYDRFLKKTDLRAEKIG
                     AAFIEQIQPVLPMDINDALVDKVVTD"
     gene            complement(1399..>2170)
                     /locus_tag="PLK08_09770"
     CDS             complement(1399..>2170)
                     /locus_tag="PLK08_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPS53827.1"
                     /translation="AEISDGDARRALTALEVGVMSIIRSAGRDDVKVKFDLNVAQESI
                     QAKALQYDRTGDMHYDAISAFIKSMRGSDPDAAVYWLARMLSAGEDPRFIARRIAILA
                     SEDIGNADPRAITVAASCYQIVERIGMPECRITLSQAAIYMATAPKSNASYMAVDAAM
                     ADVRNNRTIPVPRHLRNAPHPGMREVFGNHVGYQYSHSYEGGISPDQDYLGVEKTYYQ
                     PTDRGYEAHIAKYLEWAQSMRTADASNDAERPKKKNTK"
BASE COUNT          452 a          606 c          569 g          543 t
ORIGIN      
        1 atggaagggg cccctgacga gtttgtaatt acggccagcc tgaaaattcc tggccgacaa
       61 gccaaagccg ttgacatacg gaatcatcct ggccggcaca caaaggtcgt aatggctgat
      121 aatgccctcc cgcgttattt tcccgcccgc gccgcgaatc gaattgagcg tatcgccgga
      181 tttgaatttg tagctaatca gatacgggtc tttggggttg acgtacggag aaggattcag
      241 gacggttctg ttcgccaggt cttcgagagc ttttctcgcg atgacagcct gcggttcggc
      301 cagaaatccg gaattgagcg ccctgttcag cagcgtgcgc gcctgtatgt aatccttatt
      361 ctgctggtaa agtacccagc cctgggcata cgcctgcctg gctgcgtcgg cattgatgcg
      421 cggcgtagtc agcggcgatg ggcctatcgc cctcgacaac gccacagtca tgcctttggg
      481 gccataagct ttgtcggtat ctttggcggc ggatttttca gaatatgccg taacaggctg
      541 tgatttttgc gcatgttcct gtgccgtgtc gtttttctgt tgagttttgt cggctgtcga
      601 aaattcctcc ggccccgaaa ttttcggata acccggctgc ggcgccggtt cgagtgcagg
      661 tggattctcc gccggcttgc ggccggacgg ccagaataca acagccagca atactcctgc
      721 cagcgccaat attgtcacat ataagccagt gttggtcttc ctggcctgcg tatacatctt
      781 catggagata cctccagtct tttccagaca atttcagaca attccaaacg acggaatata
      841 aaatttttta aacgggaatt gacgcgccag ctcacgtcgc catgtcaatc cgtaactacc
      901 ttgtccacaa gcgcatcgtt tatatccatc ggcaaaaccg gctggatttg ttcgataaaa
      961 gcggcgccga ttttttcagc ccgcaaatca gtttttttca gaaaacggtc ataaaagccg
     1021 ccgccgcggc ccagtcggcc gccgtttctg tcaaacgcca accccggcac aacgataaag
     1081 tctatttcat caataaaaat ctctctgccc agcggactgc gaagacccat ttcggggctg
     1141 aaaaggctca tattttcagg cctgatttct atcgccctca tttcacggtc gagcggactt
     1201 gtaggggcgc atacggtttt cccagcccgc caggcctgcc gggccaatcc gccgagcctg
     1261 acttcaccgt caatcggcag ataaagcatt atgcaggctg cctgcaaaaa cccaggcgta
     1321 tcggctaatc gccggcagat tacatcgctt ttgcattgca attccggctc gcaaagtttt
     1381 gccaatgctg cccgcacatc acttcgtatt tttctttttt ggtcgttcag catcattact
     1441 cgcatcagca gtccgcatgg attgcgccca ctccagatat tttgcaatgt gggcttcata
     1501 gccccggtcg gtcggctgat aataagtttt ttccacgccc aggtaatcct ggtccggcga
     1561 aatgccgcct tcataactgt gcgaatattg gtaaccgacg tgattgccga aaacctcgcg
     1621 catgccgggg tgcggggcat tccgcaaatg tctcggaacg ggtatcgtgc gattgttgcg
     1681 aacgtcagcc atggcggcgt cgacagccat atacgaggcg ttggacttgg gtgcggtggc
     1741 catataaatt gcggcttgag ataatgttat gcgacattcc ggcatgccta tccgttcgac
     1801 tatctgatag cagctcgcag cgacggtaat ggctcgcggg tcggcattgc cgatgtcttc
     1861 actggcgagg atggctattc ggcgggcaat gaaccgcggg tcttcgccgg cggagagcat
     1921 gcgagccagc cagtaaaccg ccgcgtccgg atcgctgccg cgcatggatt ttatgaacgc
     1981 acttatggcg tcgtaatgca tatcgcctgt acggtcgtac tgcagggcct ttgcctgtat
     2041 ggattcctgt gccacgttca aatcaaattt cactttgacg tcatctctgc cggctgagcg
     2101 gattatgctc atcacgccaa cttccagggc cgtcaatgcg cggcgggcgt ctccatccga
     2161 tatctccgcc
//
LOCUS       DAONFI010000360         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_67419_length_2167_cov_4.864110, whole genome shotgun sequence.
ACCESSION   DAONFI010000360 DAONFI010000000
VERSION     DAONFI010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67419_length_2167_cov_4.864110"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1128)
                     /locus_tag="PLK08_09775"
     CDS             complement(208..1128)
                     /locus_tag="PLK08_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS53828.1"
                     /translation="MNSRERVLCALSHKTPDRVPVDMWFNDDSWAKFSNAVGAEDLSD
                     FFRTDVRRIFFNPVNPLAALEPYPFPDRKFAFCTVENLKAKTEAIHEKNLAVEGCPGS
                     VCFEIATAKCGGLENILVSLAEGDSSLKKLLKEIADAKIDMMMKYASAGVDVIGTGDD
                     FGTQRGLMMSPDMWREWFKPFIREAVEKVKNINPETAVKFHSDGNIWDIIPDLIECGI
                     DIINPVQPECMNIGELKKSFGNDICFWGGIGTQHVLPFGTPDEVKEDVRKTCSIMGGS
                     GGFILCSSHMVQEQTPLENVFAFFEAAKQC"
     gene            complement(1177..1962)
                     /locus_tag="PLK08_09780"
     CDS             complement(1177..1962)
                     /locus_tag="PLK08_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPS53829.1"
                     /translation="MITIDLTGKVALVIGGSRGIGAGIVKTFADAGAAVAFTHTGNPA
                     YAAQLGALLDEQRADGHLVTAFACDAQNSKETQNVVDQVLKLYGKIDILVANVGKNIA
                     RSVEATTDEDWIQSVDINLSSTFYAVRAVIRPMLDAGGGRIFLIGSSAAFDGGGGSLD
                     YAAMKAALAGMMRYLMKNYSSKKILTNVIHPCVIETDLLKVRYSDSEKKKQLISQVPV
                     GRLGTPDDIGRLAAFLASDFGDFICGQEILVDGGRTFFRPAGT"
     gene            complement(1973..>2167)
                     /locus_tag="PLK08_09785"
     CDS             complement(1973..>2167)
                     /locus_tag="PLK08_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53830.1"
                     /translation="PESKIPLCKETKQIRDEGMKRGAIFGLAGVRKNLLKIKPPLIIT
                     TSECDEVLRIFGEALKAVLR"
BASE COUNT          561 a          598 c          458 g          550 t
ORIGIN      
        1 atggcggaat tggcagacgc gcaaggttta ggtccttgta tccgtaagga tgtatgggtt
       61 cgactccctt tcctcgcaat atcctgaaaa acacagcgga aaaattttaa ttccggcatc
      121 cacgtcgaac agagttaaaa gtaacgacgg ccgacttttt tattagaacc tatcccaaaa
      181 acatgggcgt cgccgaaatc cggcaggtca gcactgtttg gccgcttcaa aaaaagcaaa
      241 aacattttcc aacggcgtct gctcctgaac catgtggctg gagcatagta taaaccctcc
      301 gcttccgccc atgatggagc acgttttgcg aacatcttcc ttaacttcgt ccggcgtacc
      361 aaacggcaaa acatgctgag tccctatgcc gccccaaaaa cagatatcgt ttccgaaact
      421 ttttttcaat tcgccgatat tcatgcattc aggctgtacc ggattgatta tatcaatgcc
      481 gcactctatc aaatcgggaa tgatatccca gatattaccg tccgaatgaa actttacggc
      541 tgtttcagga ttgatgtttt taactttttc aacagcctcg cgaataaacg gtttaaacca
      601 ttctcgccac atatccggac tcatcatcaa gccgcgctga gtgccaaaat catcgcctgt
      661 gccgataaca tccacgcctg cgctggcata tttcatcatc atatctatct tggcatcggc
      721 aatttctttc aaaagttttt tcaacgacga gtcgccttca gccaaactga ctaaaatatt
      781 ctccaacccg ccacatttcg cagtggcaat ttcaaagcac accgagcctg gacagccctc
      841 tacggccaga ttcttttcat gaatagcctc tgttttcgcc ttgagattct caacagtaca
      901 aaaagcaaat tttctatcgg ggaaaggata cggctctaaa gccgccagag gatttaccgg
      961 attaaaaaag attctccgta catccgtccg gaaaaaatcg ctcaagtctt cagccccgac
     1021 agcattggaa aatttcgccc atgagtcatc attaaaccac atatcaaccg gaactctgtc
     1081 cggcgtttta tgggacaacg cacacaagac tctttctctt gagttcattt catttctcct
     1141 gaagataaag tataacacgc atacgtcatt atcatgttac gtgccagccg gacggaaaaa
     1201 agttctgccg ccgtcaacga gtatctcctg gccgcaaata aaatcgccga aatcgctcgc
     1261 cagaaacgcc gccaatcttc caatatcatc cggcgtgcca agcctgccga ccggaacctg
     1321 cgatatcagc tgttttttct tttccgaatc gctataccgt actttaagca agtcggtctc
     1381 tatcacgcac ggatgaatga cgttcgtcag gatttttttt gaagaatagt ttttcatgag
     1441 gtatcgcatc atgccggcca acgccgcctt catcgcggcg taatcaagcg atccgccgcc
     1501 gccatcgaac gccgctgaag aaccgataag aaaaatccgg ccgccgccgg catcaagcat
     1561 cggccgtatt acggctctga cggcataaaa tgtagacgaa agattaatat cgacggattg
     1621 aatccagtct tcatcggtgg tggcttcgac cgagcgggcg atatttttcc cgacattcgc
     1681 caccagaatg tcgattttgc cataaagttt caatacctgg tctacgacgt tctgcgtttc
     1741 cttggaattc tgcgcgtcac aggcaaaggc tgtcaccaga tgcccgtcgg cgcgctgctc
     1801 gtcaagcagc gcgccaagct gggccgcata tgccggattg cctgtatgag taaaggccac
     1861 ggctgcaccg gcgtcggcaa aggttttgac tatccccgcg ccaatgcctc gcgagccgcc
     1921 gattacaagc gccaccttac cggtcaaatc aatcgttatc atagcttttg cctcaacgca
     1981 atacggcttt aagggcttcg ccgaaaatac gcagaacctc atcgcattct gacgtcgtta
     2041 taatgagcgg aggttttatc ttcaaaaggt ttttacgcac gccggctaac ccgaagatgg
     2101 cgccgcgttt catgccttca tcacgaatct gcttcgtctc tttgcacagc ggtatcttgc
     2161 tttcagg
//
LOCUS       DAONFI010000361         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_67425_length_2167_cov_3.715436, whole genome shotgun sequence.
ACCESSION   DAONFI010000361 DAONFI010000000
VERSION     DAONFI010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67425_length_2167_cov_3.715436"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2167
                     /locus_tag="PLK08_09790"
     CDS             <1..>2167
                     /locus_tag="PLK08_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPS53831.1"
                     /translation="RCRRPVQGTANRPLKSLTDMIKGKQGRFRENLLGKRVDYSGRSV
                     IVVGPELKLNQCGLPKKIALELFQPFIIRKLKERGLADTIKSAKKMLERRDQEIWDVL
                     EEVIYQHPVMLNRAPTLHRMGIQAFEPILVEGNAIRVHPLVCKGFNADFDGDQMAVHL
                     PLSVEAQTEAHVLMLATNNIFSPANGSPMMAPTQDMVLGIYYLTSDRGKTFADKSLQR
                     VEYKGDKYPVFCNLHQAITAHAMGRIGMHALVLTRVEKGLEVHESSGTVFAKDGRIIT
                     TVGRMIFNDILPKGIGYYNYPLGSKGAGKVISNCYERLGRAATLELLDNIKSIGFRYS
                     TLAGLSFGITDARIPDNKKEIIDAAQKRVDQIQADYEHDAITEGERTNQIVDVWIHAR
                     EEVTGSLFKALKEDKGPNPANDSGYLNPVFLMTDSGARGKIDQIRQLAGMRGLMSKPS
                     GEIIETPIKANFREGLNVLEYFSSTHGARKGLADTALKTADSGYLTRKLADVAQNVIV
                     NEIDCGTLRGVKKVAIYKGEQIDVPLRDLIIGRVARETIADPVHDRIIVKENQIITAE
                     AAKAIEELGIEEISVRSPLTCESPLGCCAKCYGLDMSTGRMVEEGMAVGIIAAQSIGE
                     PGTQLTMRTFHTGGTATRAVIESEWLAPVSGKVVYRELGAVPAPDNKKDFVALKRQGE
                     IAIVDEKNRELEKYKVSYGSHIVIADGGKVKAGQRVVWWD"
BASE COUNT          532 a          588 c          603 g          444 t
ORIGIN      
        1 cgttgccgtc gtcctgttca gggcacggct aaccggccgc tgaaatcgct gacagacatg
       61 ataaaaggca aacagggccg cttccgtgag aatctgctcg gcaaacgagt ggattattcc
      121 ggccgaagcg ttatcgtcgt tggccctgag cttaaactca accagtgcgg cttgcccaag
      181 aaaatcgcat tggaactgtt tcagccgttt atcatccgca aactcaaaga gcgcggcctt
      241 gccgatacaa tcaaatcggc caagaaaatg ctcgaacgtc gcgatcagga aatatgggac
      301 gttctggaag aagtcatata tcagcatcct gtcatgctca accgtgctcc taccctgcac
      361 cgaatgggta tccaggcgtt tgagccgatt cttgtcgaag gcaacgccat ccgcgttcat
      421 ccgctggtct gcaaaggatt taatgccgac ttcgacggcg accagatggc tgtccatctg
      481 ccgctcagcg ttgaggcgca gacggaagcc catgtgctca tgcttgcgac caacaacatc
      541 ttttcgcctg ccaacggcag tcctatgatg gctccgacgc aggacatggt gctcggcata
      601 tattatctca ccagtgaccg cggcaagacg tttgcggaca aaagccttca gcgcgtcgaa
      661 tacaagggcg acaaataccc cgtattctgc aacctgcatc aggctatcac agcccatgcg
      721 atgggccgta tcggcatgca tgccctggtg cttacgcgtg ttgaaaaagg ccttgaagta
      781 cacgaatcca gcggcacggt ctttgcgaaa gacggcagaa tcatcacgac cgtcggccgc
      841 atgattttca atgatatatt gcccaagggc ataggctatt acaattatcc gctgggtagc
      901 aagggcgcag gcaaggtcat cagcaactgt tacgagcggc tgggccgtgc ggcgacgctc
      961 gaattgcttg acaatatcaa gtcgataggg ttcaggtatt cgacgctggc gggcttgtcg
     1021 ttcggcatta ctgacgccag aattccggat aacaagaagg aaatcattga cgcggctcag
     1081 aaacgcgtcg accagattca ggcggattat gagcacgatg ccataaccga aggcgaacgt
     1141 accaaccaga tagtcgacgt ctggattcac gcgcgtgaag aagtcacggg cagccttttc
     1201 aaggctctca aggaagacaa gggccccaat cctgcgaacg acagtggtta tctgaaccct
     1261 gtgttcctga tgacggattc cggcgctcgt ggcaagatag accagattcg acagttagcg
     1321 ggcatgcgtg gcttgatgtc aaagccgtcg ggcgaaatca tcgaaacgcc aatcaaggct
     1381 aacttccgcg aaggcctcaa cgtgcttgaa tatttctcat ccacgcacgg tgcccgcaaa
     1441 ggcttggctg ataccgcgtt gaagacggct gactctggtt accttacccg taaactggcg
     1501 gacgtggcgc agaatgtcat agtcaatgaa atcgactgcg gcactcttcg aggcgtcaag
     1561 aaggtcgcca tatacaaggg cgagcagatt gacgtgccgt tgcgtgacct catcatcggc
     1621 cgtgtagccc gcgagactat cgccgacccg gtacatgacc gcatcatagt caaggaaaac
     1681 cagataatca ccgctgaagc cgccaaggca atcgaggaac tcggcataga ggaaatttcc
     1741 gtccgcagcc cgctgacttg cgaaagcccg ctcggctgct gtgccaagtg ttacggcctt
     1801 gatatgtcca ccggccgaat ggtcgaagaa ggcatggctg tgggtataat tgcggctcag
     1861 tccatcggcg agccaggcac gcagttgaca atgcgtacgt tccataccgg cggtacggct
     1921 acccgtgcgg ttatcgagag tgaatggctg gcaccggttt ccggcaaggt cgtatatcgc
     1981 gaactcggcg ctgtgcctgc tccggacaat aagaaggact ttgtcgctct gaaaaggcag
     2041 ggcgaaatcg ccatcgtcga cgagaagaac cgcgaactcg aaaaatacaa ggtttcttac
     2101 ggttctcata tcgtgatagc cgacggcggc aaggtcaaag ccggccagcg cgtcgtctgg
     2161 tgggatc
//
LOCUS       DAONFI010000362         2154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_68078_length_2154_cov_4.422582, whole genome shotgun sequence.
ACCESSION   DAONFI010000362 DAONFI010000000
VERSION     DAONFI010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68078_length_2154_cov_4.422582"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLK08_09795"
     CDS             complement(<1..439)
                     /locus_tag="PLK08_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53832.1"
                     /translation="MIQHAGRVHIIENVCDNDGHGKKVLVALSGGVDSAAAACILLER
                     GFEVTGAFLCMERPAEDCSARTCCSPQDAWDAAAVADKLGIRLLKIPAAGYFEKIMSD
                     FADEYATGRTPNPCIHCNVLLKFGHLAKIADDAGIDYIATGHYA"
     gene            complement(436..675)
                     /locus_tag="PLK08_09800"
     CDS             complement(436..675)
                     /locus_tag="PLK08_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53833.1"
                     /translation="MNVQYFFIHEIRDVLFAYRVSSRLAAAQFGRVNNIILSTLEDLR
                     EIAATRNKSLYLAGFPNERRTVQNAANALSAVSAA"
     gene            complement(917..>2154)
                     /locus_tag="PLK08_09805"
     CDS             complement(917..>2154)
                     /locus_tag="PLK08_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS53834.1"
                     /translation="TTARAVPIYATSSYVFHDADHAANLFGLREFGNIYSRLMNPTND
                     VLEKRLAALDGGAAGLAFASGQAAITAAIVTITHSGQNFISSTNIYGGTWTLFVHTLQ
                     KLGIEVRFFDPAHPEQIASLVDENTRLVYIESVGNPKNDVPDFKAIADAAHAKGLPLV
                     VDNTVLTPYLFRPIEHGADIVVYSTTKFIGGHGVHIGGAIVDAATFPWADNPKKWPEF
                     NAPDPAYHGAIFTEALKPIGNISYIIHIRTHWLRDTGAAQSPFASFLFLLGLETLHVR
                     LERHSSNALKVAQWLSNHPAVEWVNYPGLPSHKQHENAKKYLKKGFGAIVGFGIRGGA
                     AAGVKFINNVKLASHLANIGDAKTLVIHPASTTHSQLTEQELASSGVSPDFVRLSIGI
                     EDVEDIIADFDQALKASQA"
BASE COUNT          502 a          566 c          553 g          533 t
ORIGIN      
        1 gcgcataatg gccggttgcg atgtaatcaa ttcctgcgtc atcagcaatt tttgcgagat
       61 gtccgaattt tagaagtacg ttgcaatgaa tgcacgggtt gggtgtacgc cccgtggcat
      121 attcatcagc aaaatccgac attatttttt caaaatatcc agctgcaggt atttttaaca
      181 gtcgtattcc aagtttgtcg gcaacggctg ccgcgtccca cgcatcctgc ggcgagcagc
      241 atgtgcgcgc ggaacaatcc tccgcaggtc tttccatgca gaggaatgct ccggttactt
      301 caaatccgcg ttcaagcaat atacaagcag cggcggcaga gtcaactccg ccgctcaatg
      361 ccaccagcac ttttttaccg tgcccgtcgt tatcacatac attttctatt atatgtactc
      421 tcccggcatg ctgtatcacg ccgcagaaac tgccgacaag gcattggcag cattctgcac
      481 agttcttcgt tcattcggaa aacccgcaag atatagcgat ttgttgcgag tcgctgcaat
      541 ctctcgaagg tcttccaatg ttgacagaat tatattattc acacgaccga actgcgccgc
      601 agccaatcgc gaactgaccc gatatgcaaa aagcacatca cgaatttcat gtatgaaaaa
      661 atattgtacg ttcatttttg tttctcctgt gtacgatgtt caaatacgcc ttgccggtta
      721 cacaaccggc ctgcttgtca aaatgttcaa aacattaatg acagtaacac atggagaaac
      781 acattcgaaa aaaactgctg cggcgcaatc tgctgttcaa tccggcaaaa actgccgacg
      841 aggcagcctt gtcccgtagt tgttccagat ttacttgttg cagattacgc cgcatttcac
      901 actttcctta acaaactcaa gcctgtgagg ccttgagtgc ctgatcgaaa tcggcgatga
      961 tgtcttccac atcctcaatg cctattgaca ggcggacgaa atccggcgat acgccagacg
     1021 atgccaactc ctgttccgta agctgcgaat gcgtggtcga cgcaggatga atgaccagtg
     1081 tcttcgcatc gccgatatta gccagatggc tggccagttt gacgttattg ataaacttca
     1141 ctccggcagc cgctccgccg cgaataccga aacctacgat tgcgccgaaa ccttttttga
     1201 gatatttttt ggcattctcg tgctgcttgt gcgacggcag gccgggatag ttcacccatt
     1261 ccaccgctgg atgattgctc aaccactgcg ccaccttgag cgcgtttgaa gagtgccgct
     1321 ccagtcgaac gtgcaacgtc tccagtccca acaggaacag gaacgatgca aacgggcttt
     1381 gcgccgcgcc ggtatcgcga agccagtgcg tgcggatgtg aatgatatat gaaatgttcc
     1441 cgattggctt gagggcctcg gtgaaaattg cgccgtgata cgccgggtct ggtgcgttga
     1501 attcaggcca tttcttcggg ttatcggccc agggaaatgt tgcggcatcg acaatcgcgc
     1561 cgccgatatg cacaccatga ccgccgatga attttgtggt ggagtatacg acgatatcag
     1621 cgccatgctc gataggccgg aaaagatatg gcgtgagtac cgtattgtcc actaccagcg
     1681 gcaaaccttt ggcgtgtgcg gcgtcggcta tcgctttgaa gtcgggcacg tcgttcttgg
     1741 ggttgccgac tgattcaata taaaccagcc gcgtattttc atccaccagc gaggctatct
     1801 gttcaggatg tgcagggtcg aagaaccgca cttcaattcc gagtttttgc agcgtgtgaa
     1861 caaacagagt ccacgtgccg ccgtaaatat tcgtagagct gatgaagttc tggccgctgt
     1921 gtgtgatggt cactatcgcc gctgttattg cagcctgtcc gctggcaaat gccaggcctg
     1981 ccgctccgcc atcaagtgcg gcaagacgct tttcgagcac gtcgttggtg gggttcatca
     2041 ggcgcgaata gatgttgccg aattcacgca gcccgaacag gttggcggcg tggtcggcat
     2101 cgtgaaacac gtagctggac gtggcgtaga tgggcacggc gcgagccgtg gtgg
//
LOCUS       DAONFI010000363         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_68131_length_2153_cov_6.725929, whole genome shotgun sequence.
ACCESSION   DAONFI010000363 DAONFI010000000
VERSION     DAONFI010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68131_length_2153_cov_6.725929"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..133
                     /locus_tag="PLK08_09810"
     CDS             2..133
                     /locus_tag="PLK08_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPS53835.1"
                     /translation="MVRRDNKFIVPRGQTVIKPYDTLLVMATDGDFRVIDQLLNDEK"
     gene            complement(141..2096)
                     /locus_tag="PLK08_09815"
     CDS             complement(141..2096)
                     /locus_tag="PLK08_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPS53836.1"
                     /translation="MAYCVATADVKPASIFSSDMVLQQEKPIKIWGTAAPAETVTVTL
                     DNESQETTAAANGKWAVTFQPRKASFSPITLTIAGNNKISFENVLVGEVWICSGQSNM
                     QFRLCEAKNAQQEIAAADYPNIRLCQVSQNVQLAPRDNFTAKWTPCSPVTAPKFSAVG
                     YFFGRDIYKSLNVPVGLISSNWGGTIVEAWTPLEYLQTDESMQHRLTVDKPLLDEKTK
                     AQRVQKYQDAMKKYYANVAECKKAAENPESAAKYSSIDFDDNAWKTMNIPCIIRQGGL
                     PNYYGYVWFRKVIDVPADWAGRDIVLHLGPIDETDATFFNGIEVGHSYVPKLRKQVRN
                     YVVPGKLVKAGKNILAVRMMNVTSYGGLYGEPANKLFAEPASAAGKDAKISLAGTWKF
                     LPAVKFAPKPGNPVNPNICSVLYNGMINPMAGFAVRGAIWYQGESNGGRGYQYRRLMP
                     LMIKAWRDRWNDQSIVFIQTQLANYLERQTQPKQSAWAELREAQLLTAQNDPLGGMAV
                     IIDIGEGNNIHPLNKQDVGKRLALQAEKIAYGKDLPFEGPIYKSMKIESNKIRLNFDG
                     VYDGLAAKGGNLKGFAIAGQDKKFFWAAAEIDGRDIIVWSDKVPAPVAVRYAWADNPE
                     CNLYNSADLPATPFRTDDWKGLSADKK"
BASE COUNT          479 a          550 c          532 g          592 t
ORIGIN      
        1 gctggtcagg cgtgacaaca aattcatcgt gccgcgcggc caaaccgtaa taaagccgta
       61 cgatacgctt ctggtaatgg ccaccgacgg cgattttcgc gtaatcgatc agttgctgaa
      121 cgatgaaaaa tgaagacggt ttattttttg tcggcggata agcctttcca gtcgtcagtt
      181 ctgaacggcg tcgcaggcag gtcagccgaa ttgtaaagat tacattccgg attgtcagcc
      241 catgcgtaac gaacagccac gggagccgga actttatccg accagactat gatatccctg
      301 ccgtctattt cagcggcggc ccagaagaat tttttatcct gtcctgcgat ggcaaaaccc
      361 ttcagattgc cgcccttagc cgcaaggcca tcatatacgc catcaaaatt caacctgatt
      421 ttgttgcttt caatcttcat ggatttataa atgggacctt cgaacggcag gtctttgccg
      481 taggctattt tctcggcttg caatgccagc cgtttgccga cgtcctgctt gttgagcgga
      541 tgaatgttat ttccctcgcc gatgtcgata atcaccgcca tgccgccaag cgggtcgttt
      601 tgtgccgtga gcagttgtgc ttctcggagt tccgcccatg cgctttgctt gggctgagtc
      661 tgccgttcta ggtaattggc gagctgggtt tggataaaca cgattgactg gtcattccag
      721 cggtcacgcc aggcttttat catcagcggc attagccggc gatactggta accccttccg
      781 ccgttcgatt cgccctgata ccatatcgcg ccgcgaacgg caaaaccggc catggggttt
      841 atcatgccgt tgtacaaaac actgcatata ttcggattta ccggattgcc cggtttgggt
      901 gcgaacttaa cagccggcag aaacttccat gtgccggcga gagatatttt tgcgtctttg
      961 ccggcggctg atgcgggttc ggcaaaaagt ttgtttgccg gctcgccgta taggccgccg
     1021 taagacgtca cattcatcat acggacagca agtatgtttt tgccggcttt gacgagtttg
     1081 ccgggtacaa cgtaattacg gacttgcttc cgtaatttcg gaacgtaaga atggccgact
     1141 tcaatgccgt taaaaaatgt cgcgtccgtc tcatcaatcg ggcccagatg caggacgatg
     1201 tcacggccag cccaatcggc tgggacatcg attactttgc gaaaccagac ataaccgtaa
     1261 taattcggca agccgccctg tctgatgata cacggaatat tcatggtctt ccatgcattg
     1321 tcgtcgaaat caatcgatga atacttggcg gcagattcgg gattttccgc agcctttttg
     1381 cattccgcga cgttcgcgta atattttttc attgcatcct gatatttctg aacccgctgg
     1441 gctttggttt tttcgtccag caacggtttg tcgacagtga gtctgtgctg cattgattcg
     1501 tcggtttgca ggtactccag cggcgtccat gcctcgacta tcgtgccgcc ccaattactt
     1561 gaaatcagac ctacaggcac attgagcgac ttgtagatat ctctgccgaa gaagtaacca
     1621 actgcggaaa atttcggtgc agtcacaggg ctgcatggag tccattttgc agtaaaattg
     1681 tccctggggg caagttgtac gttctgggag acctgacata accgtatgtt cggataatcc
     1741 gccgccgcaa tttcctgttg tgcattcttt gcttcacata gacggaattg catatttgac
     1801 tggccgctgc atatccacac ttcgcctaca aggacatttt cgaatgagat tttattattt
     1861 cctgcgatag tcagagtaat cggcgaaaaa gaggcctttc gcggctggaa agtcacagcc
     1921 catttgccgt ttgcggcggc agtggtttcc tgagattcgt tgtccagcgt gactgtaaca
     1981 gtttccgccg gagctgccgt accccatatt tttatgggct tttcctgttg aagaaccata
     2041 tcgctactga aaatggatgc tggtttgacg tcggctgtcg ccacgcagta tgccatcaaa
     2101 caaacggctg aaattacgct ataaaacctt ttcatcctgc cacctcttac tta
//
LOCUS       DAONFI010000364         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_68475_length_2146_cov_4.196557, whole genome shotgun sequence.
ACCESSION   DAONFI010000364 DAONFI010000000
VERSION     DAONFI010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68475_length_2146_cov_4.196557"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..2027
                     /locus_tag="PLK08_09820"
     CDS             270..2027
                     /locus_tag="PLK08_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53837.1"
                     /translation="MIVAHVTHEAVENIGGIGTVIAGLVTAEAYRKKVSRSFLIGPKF
                     AGEKTLADRLGPHGEVLYCRADGKIGGEYEKIIRPVEERYGCEIDFGRRLIIEPYSGK
                     NVLIDVILVDVSVVNIERLNTFKGRLFEKFGVTSDRFENIWEYEQYVRLAEPACDTLR
                     ALINGARNPVVILGHEFMGMPTVLKAKLDMPESRAYFHAHEVASVRPVVEKRSGHDTM
                     FYNVMSAARRKGLTLEEVFDEVAVNFKHRLVKAAKYCDGVLAVGDFVREELEFMSSDF
                     TESNVHLVYNGIPSNHITLDDKIRSRTMLCDYARKLLGYCPRWVFTHVARPVLSKGIW
                     RDLRVMHELDAMLQQRGETAVYFMLGTLGGVRRPSDVMRMEKDYDWPVTHRLGYPDLC
                     GGEDVISDNIDAFNVSHQAVHAILINQWGFNRQACGERMDVDTTIADLRKGCDIEFGM
                     SVYEPFGISQLEGLTYGALSVISNICGCAGFVGLTERNQPTGNVIVGDFVRQKNIADE
                     IYRKNPDKAVQTLLSLGREQRDQIEKMEGRRLARQIDSLLPRSMGEQQKMLEGGLELA
                     DKMSWQRVVSEMLMPVIAG"
BASE COUNT          526 a          478 c          619 g          523 t
ORIGIN      
        1 gcgtttatcg aaataggcga cggtacgctg atgatatctg tgccttaccg tgatgaacgt
       61 aaatggaatt ataatctctg gcttgacaaa gccttgtgca tcgccagggc tctggctgcc
      121 agcctggaag aaaatgcatc atttcaaatt tcgtaatgac acagcccgcc atgtatttgt
      181 ataatacccc tctggtttgc tgcggctgag ccgcaggaac tgctctagtc aagccggatt
      241 ttgtttaaat tgattggata tttcatccca tgatagtagc acatgtaaca cacgaagctg
      301 ttgaaaacat tggcggaata ggtacggtca tcgccggcct tgttaccgcg gaagcatatc
      361 gaaaaaaagt ttcgaggtca tttctgatag gccccaaatt cgccggcgaa aaaacgctgg
      421 ctgacaggct tggcccgcat ggcgaagtgc tgtactgcag ggcggatggc aaaattggcg
      481 gcgaatatga aaaaataatt cggcctgtcg aagaacggta tggctgtgaa atcgattttg
      541 ggcgacggct gataattgag ccttacagcg gcaaaaatgt gctgatagac gttattcttg
      601 tcgatgtgtc cgtggtgaat atcgagaggc tcaacacgtt caagggccgg ctgttcgaga
      661 aattcggagt gacgtccgac aggtttgaga atatatggga gtatgagcag tatgtccggc
      721 tggctgagcc ggcctgcgat acgttgcgtg cgcttatcaa cggcgcccgc aatcctgtgg
      781 tgattctcgg ccatgaattt atgggcatgc ctactgttct gaaagccaag ctcgatatgc
      841 ccgaaagccg cgcctatttt catgcccatg aagtcgcatc cgttcggccg gttgtcgaaa
      901 aacgctccgg ccatgatacc atgttctata acgtcatgtc tgcggcacgc cgcaaaggat
      961 tgactctgga ggaagttttt gacgaggtgg ccgtcaattt caaacatcgg ctcgtcaagg
     1021 cggctaaata ttgcgacggc gtgttggcgg tgggcgattt cgttcgtgaa gaacttgagt
     1081 ttatgtcgag cgattttacc gagtcgaacg ttcatcttgt ttataacggc ataccgtcta
     1141 accatattac actggacgac aagattcgca gccgtacaat gttgtgcgat tatgcccgca
     1201 agctgctggg ttattgtccg aggtgggttt tcacgcatgt ggcgaggcct gtgctgagca
     1261 aggggatatg gcgagacctt cgcgtaatgc acgagcttga tgccatgttg cagcagcgcg
     1321 gcgaaacggc tgtctacttc atgcttggta cgcttggcgg cgtcagacgg ccctcggacg
     1381 ttatgcggat ggaaaaggat tacgattggc cggttacgca tcggctggga tatccagacc
     1441 tgtgcggcgg cgaagatgta atatcggaca atatcgacgc gttcaatgtt tcgcatcagg
     1501 ctgtccacgc gatactcata aatcagtggg gctttaaccg ccaggcttgc ggcgaacgca
     1561 tggatgtcga taccaccatt gcagacctcc gcaaaggctg tgatatcgag ttcggcatga
     1621 gcgtttacga gccgtttggc ataagccagc tggaaggcct tacctatggt gcgttgagcg
     1681 tcatttcgaa catctgcggc tgtgccggct ttgtcggatt gaccgagcgg aatcagccga
     1741 ctggaaacgt gattgtcggc gactttgtcc ggcagaaaaa tattgccgat gaaatatata
     1801 ggaaaaatcc tgataaagcc gtccaaaccc tcctatcatt aggtagagaa cagcgcgacc
     1861 agattgaaaa aatggaaggc cgacggctgg cgagacagat tgacagcctt ttgccgcggt
     1921 cgatgggtga gcaacagaaa atgctggaag gcgggttgga actggctgat aaaatgagtt
     1981 ggcagcgcgt ggtaagcgag atgctcatgc cggtaatcgc cggctagtta aaattgtttt
     2041 tttacggggg gcgtggagag aagaactaaa aatgagattg tcaccggcag ctgagttggc
     2101 gataagaggc tcactgattt tggcaaaacg ccataatcag gggcct
//
LOCUS       DAONFI010000365         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_68476_length_2146_cov_4.019608, whole genome shotgun sequence.
ACCESSION   DAONFI010000365 DAONFI010000000
VERSION     DAONFI010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68476_length_2146_cov_4.019608"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLK08_09825"
     CDS             <1..343
                     /locus_tag="PLK08_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS53838.1"
                     /translation="GNPICAAAALASAELIEEENLVQAAIDNGRETLEILTNAKLPGV
                     MEIRGKGMMLGIAFDKNVKAAAAAAAALDRGLLTCTGKNNTLRFVPALNIDGNTLHKG
                     LELLIAAIKSI"
     gene            575..1486
                     /locus_tag="PLK08_09830"
     CDS             575..1486
                     /locus_tag="PLK08_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPS53839.1"
                     /translation="MTGPQLVVMAAGMGSRYGGLKQIDPIGPNGEIIIDYSIYDAMQA
                     GFEKVIFIIRRDIEKDFRDMVGRHIEPIIDTQYVFQQLDMLPAGMNVPENRVKPWGTG
                     HAVLCAKDAVTAPFAVINADDFYGRGSFAAIGNYLKNARDARYYDYSMVGYTLKNTVS
                     EFGHVARGCCQVKDDKLASIVERTRIQKFTDGIKYSDDDGTTWINISPETIVSMNMWG
                     FTLSFMDELGPRFERFVRDNAANPKAEFFIPLAVNQLMEEDKAAVTVLPCNEKWYGVT
                     YREDKPVVERAIRNMIAQKTYPKNLWA"
     gene            1505..>2146
                     /locus_tag="PLK08_09835"
     CDS             1505..>2146
                     /locus_tag="PLK08_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mucin desulfatase"
                     /protein_id="HPS53840.1"
                     /translation="MNYDLRKIAEQFDIEGKFVSAVPYGTGHINDTFRSIFSNGVERH
                     FIHQRINHKIFTEPEKLMDNISRVTKHLRKKIVAAGGNPMRQCLTLVPTKDGRNFCIM
                     DGNYWRTYVFIEGAKTYDLVEKPQHIFNAAWAFGNFQKLVSDLTQPRLHDTIPDFHNT
                     IRRFEQFETALNGDIASRAAGCKPEIDFAMARREMAGVIVNALKNGEIPERVTH"
BASE COUNT          623 a          561 c          527 g          435 t
ORIGIN      
        1 cggcaatccg atatgtgcag cggcggcact tgccagcgcg gaactcatag aagaggaaaa
       61 cctcgtacag gcggctatcg acaacggcag ggaaacattg gaaattctca caaatgccaa
      121 gctgccaggc gttatggaaa tacgcggcaa aggcatgatg ctgggcatag ctttcgacaa
      181 aaacgtaaaa gcagcagctg cagcggcagc tgcactagac agaggcctct taacctgcac
      241 gggcaaaaac aataccctgc gtttcgtgcc ggcattaaat atcgacggta ataccctgca
      301 caaagggctt gaattgttga ttgccgccat caaaagcata taaaagataa aaaaaatccc
      361 acatccaaaa ttcggctgtc cctgcaatta atgtagcggc gccggaggag agagtggatg
      421 tcgttgtttc cagctttgcg acaggccttt taacttgctt ttctggcagt tttgacatat
      481 tttgtaattt ttatgcgcaa tgtgcagaga agctctctgt gctgccggta tatgcaatat
      541 tcacaaaatt acaggacaga taaggaaaac agcaatgaca ggaccgcagc tggtagttat
      601 ggccgccggc atgggcagcc gctacggcgg actcaaacag atagacccga taggcccaaa
      661 tggcgaaata atcattgatt attcgatata cgacgccatg caagccggct ttgaaaaggt
      721 aatatttata atccgccgcg atatcgaaaa agacttccgc gacatggtcg gccgccatat
      781 cgagccgatt atcgatactc aatacgtatt tcagcagctc gatatgctgc cagccggcat
      841 gaacgttccc gaaaatcgcg tcaaaccctg gggcacgggc catgccgtgc tttgcgcgaa
      901 agacgcggtg acggctccgt tcgcagttat aaatgccgac gatttttacg gcagaggcag
      961 ttttgcggct atcgggaatt atctcaaaaa tgcccgcgac gccaggtatt acgactattc
     1021 aatggtcggt tacacgctca aaaacactgt gagcgaattc ggccacgtag cccgcggctg
     1081 ctgccaggta aaggatgaca aactggctag catagtcgaa cgcacacgaa tccagaaatt
     1141 caccgacggc ataaaataca gcgacgacga cggcacgacg tggataaaca tttcaccgga
     1201 aaccattgtc tccatgaaca tgtggggatt caccctgagt ttcatggacg aactcggccc
     1261 acgattcgag cgtttcgtgc gggataacgc cgccaacccg aaggccgagt tcttcatccc
     1321 gctggcggtc aaccagttga tggaagaaga caaagccgcc gtaaccgttc tgccctgtaa
     1381 tgaaaaatgg tacggcgtaa cgtatcgcga agacaaaccg gtcgtcgaac gggctatcag
     1441 aaacatgata gcccaaaaaa cttatcccaa aaatctctgg gcgtaaaaga gaggaacatc
     1501 cgtaatgaac tacgacctca gaaaaatcgc cgaacaattt gacatcgagg gcaaattcgt
     1561 ttctgccgtg ccttacggga cgggacacat caacgatact tttcgcagca tattcagcaa
     1621 cggcgtcgag cgacacttca tacatcagcg cataaatcac aaaatcttca ctgagccgga
     1681 aaaactcatg gacaacatct cgcgggtcac caaacacctg cgaaagaaaa tcgtcgccgc
     1741 cggcggaaat cccatgcgac aatgcctcac gctcgtgccg acgaaagacg gccgaaattt
     1801 ctgcatcatg gacggaaatt attggcgtac ttacgtattt atcgaaggcg ctaaaactta
     1861 cgacctcgtt gaaaaaccgc aacacatctt caacgcagcg tgggcgttcg gcaattttca
     1921 aaaactcgtt tccgacctca ctcagccgcg tctgcacgac acgataccgg attttcataa
     1981 cacgatacgg cgattcgaac agtttgaaac ggcgttgaac ggcgatatcg ccagccgcgc
     2041 ggccggctgc aaaccggaaa tcgattttgc catggccagg cgcgaaatgg ccggcgtaat
     2101 cgtcaatgcc ctgaaaaacg gcgaaatccc cgaacgcgtc acgcac
//
LOCUS       DAONFI010000366         2138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_68835_length_2138_cov_3.811810, whole genome shotgun sequence.
ACCESSION   DAONFI010000366 DAONFI010000000
VERSION     DAONFI010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68835_length_2138_cov_3.811810"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLK08_09840"
     CDS             <1..330
                     /locus_tag="PLK08_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HPS53841.1"
                     /translation="YKMPGMSGLDVLKEVKQIWIYKKNTQPLAKRNAGCVFKNPRGMS
                     AGALIDKAGMKGKRVGGAYVSEKHANFIIADEGATAFDVLRLIDMIRDAVVREFNVNL
                     EKEIEVW"
     gene            389..1366
                     /locus_tag="PLK08_09845"
     CDS             389..1366
                     /locus_tag="PLK08_09845"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPS53842.1"
                     /translation="MVSFEAQPVIEFYDITVLMGGISSEREVSINSGTAIADALEKVG
                     HTVTRADISPQDTSALDREGMDLVFIALHGEFGESGEVQQLCEDRKLRYTGSGPHASA
                     MGMDKAAAKMIIRQGGLRTADWAVIEEYDSQDAIDAKLAPLGKSLVVKPVDSGSSVDV
                     HLCKGRAGLRESLASVVDIYGRALVETFIEGREFSVSLLIDRALPVMEIKSTHEMFDY
                     EAKYVDNTTTFTFEHDLPEKVQAEMRCMALSMFNLLGCRDFSRVDFMVDRENKPYILE
                     INTIPGFTSHSQLPSAARRIGMEMTGLVDLVVREAMKRDICNCCVASEN"
     gene            1374..>2138
                     /locus_tag="PLK08_09850"
     CDS             1374..>2138
                     /locus_tag="PLK08_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53843.1"
                     /translation="MAAKTSKNRRRQSNLLRRLSEWRKTENVRGNVLVVMILLLAAGV
                     LAAVLFCEMQRKMLSMRQEKVKVCYGVVFLEDDVPAWMPPQLLQIIRDEISPAKLDFN
                     DEKLCPKVLALAKKNPWIRKIKEIRKIRKGEKIGQVLVSAEFRRPFVRVSYNHMNYFV
                     DDEGVLLPARQVPQWKAVVDGEVFYYIDPEDIPVAVRPLLERFMIICGAEAPAPQVGQ
                     KWPGDDIQAGLKLAKLVTTRSYARQISVIDIRNHKKR"
BASE COUNT          551 a          509 c          604 g          474 t
ORIGIN      
        1 tacaaaatgc ccggaatgag cggactggat gttctcaaag aagtcaagca aatctggatt
       61 tacaagaaaa atacccagcc gctcgccaaa cgcaacgccg gctgcgtatt caaaaatccg
      121 cggggtatga gcgcaggagc gcttatcgac aaggctggca tgaaaggaaa acgcgtcggc
      181 ggagcttacg tgtctgaaaa acatgccaac ttcataatag ccgatgaagg agcgacggct
      241 ttcgatgtgt tacgactcat cgacatgata cgcgacgccg tcgtacgcga attcaacgtt
      301 aatctcgaaa aggaaatcga ggtatggtga cggatttctg tgcgtacatg ttcatattta
      361 ccccaacggc caggccatgg agggcatcat ggtcagtttc gaggcacaac cagtgattga
      421 attttacgat attaccgtat taatgggcgg catttcgtcc gaacgtgaag tttccatcaa
      481 cagcggcact gccattgcgg atgcccttga aaaagtcgga cacaccgtaa cacgtgcgga
      541 tatttccccg caggacacgt cagctctcga ccgcgaagga atggatttgg tatttatcgc
      601 gcttcacggc gagttcggcg aatccggcga agttcagcag ctttgcgagg acaggaaact
      661 gcgttatacc ggctccggtc cgcatgcaag cgcgatgggc atggacaagg ccgccgccaa
      721 gatgataatc cgccagggcg gattgcgcac cgccgattgg gctgttatcg aagaatatga
      781 ttcgcaggat gctatcgacg caaaactcgc tccgcttggc aagtcgctcg tagtcaaacc
      841 cgtggacagc ggttcgagcg tggatgtgca tctgtgtaag ggtcgcgcgg gcttgcgaga
      901 atcgttggcg tccgtggtgg atatttacgg cagagcgctg gttgaaacat tcatcgaagg
      961 cagggaattt tccgttagtc tgcttatcga ccgtgcactg ccggtgatgg aaataaaatc
     1021 cacgcatgaa atgttcgact acgaagcgaa gtacgtcgac aacacgacca cgtttacctt
     1081 cgagcatgat ttgccggaaa aagtgcaggc tgaaatgcga tgcatggctt tgagcatgtt
     1141 caatctgctc ggctgccgcg atttttcacg tgtggatttc atggtggaca gggaaaacaa
     1201 accgtatata ttggaaataa atacaattcc aggttttacg tctcattcgc aactgccgtc
     1261 ggcggcaagg cggattggca tggaaatgac aggccttgtt gacctcgtcg tacgcgaggc
     1321 gatgaaacgc gacatctgca actgctgtgt cgcgtcggaa aactgagctg aatatggcgg
     1381 caaagacatc aaaaaacaga cggcggcaaa gcaatctgct cagacggttg tctgagtggc
     1441 ggaagacgga gaatgtccgc ggcaatgtgc tcgtggtcat gattcttctg ctggctgccg
     1501 gcgtactggc cgccgtattg ttctgtgaaa tgcagcgtaa aatgctttca atgcggcagg
     1561 agaaagtcaa agtctgctat ggcgtggtgt tcctcgaaga tgacgtgccc gcctggatgc
     1621 cgccgcagct tctgcagatt atccgcgatg aaatttcgcc ggcaaaactg gatttcaacg
     1681 atgaaaaact ttgcccgaaa gtgcttgcgc ttgcgaaaaa gaatccctgg atacgcaaga
     1741 ttaaggaaat acgtaaaatc cgcaagggcg agaaaatcgg ccaggtgctg gtgtcggctg
     1801 aatttcgaag gccattcgtg cgtgtgtcgt ataatcacat gaattatttt gtggatgacg
     1861 agggagtgct tctgcctgcg cggcaggtgc cgcagtggaa ggctgtagtt gacggcgagg
     1921 tattttatta catcgacccg gaagacattc ccgtagcagt acggccattg ttggagcggt
     1981 ttatgataat ttgcggcgcc gaagcacctg cgccgcaggt cggccagaaa tggcccggcg
     2041 acgatatcca ggctggcctt aaacttgcaa aactcgtaac cacccgctca tatgcccgcc
     2101 agataagcgt aatcgacatc cgaaatcaca aaaaacgc
//
LOCUS       DAONFI010000367         2135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_68985_length_2135_cov_4.065385, whole genome shotgun sequence.
ACCESSION   DAONFI010000367 DAONFI010000000
VERSION     DAONFI010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68985_length_2135_cov_4.065385"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLK08_09855"
     CDS             <1..266
                     /locus_tag="PLK08_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53844.1"
                     /translation="MREVTPAIEALYARMDEIIEEELQRAAVKLASHPDNTEDMEIIK
                     ASLWRAFRKFCHPAAENLRKASAAKTAAAQAKILKQLFDLNCE"
     gene            336..1691
                     /locus_tag="PLK08_09860"
     CDS             336..1691
                     /locus_tag="PLK08_09860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPS53845.1"
                     /translation="MNEEVAYTVEAVCKKLLAAGYIFHDTETDAWPACASLLCKNDVS
                     LIVTPLPDVIGEVHMNVLSHFTSKHSGVIMVGTLPAGSPAIRNLFSRLGGRYVYINPQ
                     EKSYLRHVSWGLFTPAPRTLGESWCKSYASGEPTPAEAAVDVRTELHKHMASHAETSA
                     FIDKVNQATGFRRPQVTWAMIIIFAAVYVIMALLYHTTQFTTAQLLKWGADQGQKIVD
                     GEYWRLLTAGFVHANFMHIFFNCLGCYYFGSAVEQYQGRARLVGIFLFAVVTGNIAGL
                     WMHPESVAIGASGGLFGLIGMMAAMTIRYYRQMPHMLRNALKKWIASILVYNLFFMII
                     PQVDISAHAGGFAGGFIMGLIAARPLGSKDRPSIATLAMAAILLILTANLGMKVIAGL
                     PAKLKKAEPQLIINLTPEQIDYLRQNNSNGMIAPMPGKPGHFYVIKLHPEYAPSVKPK
                     K"
BASE COUNT          591 a          535 c          530 g          479 t
ORIGIN      
        1 acatgcggga agtcacgccg gcgatagagg ctctttacgc acgcatggat gaaatcatcg
       61 aggaagaact ccagcgggca gccgtcaaat tagcatctca tcccgataac acggaagata
      121 tggaaataat caaggccagc ctctggcgtg cgttcagaaa attctgtcat ccggcggcgg
      181 aaaatctccg caaagcctca gccgccaaaa cagccgccgc acaagctaaa atcctcaaac
      241 agctattcga cttgaactgc gaatgacagc tccggatttc taaaaaaaat tcctttggct
      301 ggaaatgccc ggggctaaat gctatagtta cccgcatgaa tgaagaagtt gcatacacgg
      361 tggaagctgt ctgcaagaag ctgctggcgg cgggatatat ttttcacgat accgaaacag
      421 atgcatggcc ggcgtgcgcg agccttttgt gcaaaaatga cgtttctctc atagtcacgc
      481 ccctgccgga tgtcatcggc gaagtgcaca tgaatgtgct gtcgcatttc acgtcaaaac
      541 acagcggcgt gataatggtc ggcactttgc cggccggctc gccggcgata cgcaatcttt
      601 tcagccgact cggcggcaga tacgtttaca tcaatccgca ggaaaaatca tacctgcgac
      661 acgtttcatg gggcctcttt acgcccgcgc cgagaacgtt gggtgaaagc tggtgcaaat
      721 cctacgcaag cggcgaaccg acgcctgccg aagcggccgt cgacgtcaga accgagcttc
      781 acaagcacat ggccagccac gcggaaacgt cggcattcat agataaggtc aaccaggcta
      841 caggctttcg tcggccgcag gtcacgtggg ccatgataat aattttcgca gccgtctacg
      901 tcataatggc gctgctttat catacgacgc agttcacgac cgcacaactc ctcaaatggg
      961 gcgcagacca gggtcagaaa atagtcgacg gcgaatactg gcggttgttg accgcggggt
     1021 tcgtccacgc gaatttcatg catatcttct tcaactgtct gggctgttat tacttcggca
     1081 gcgcggtgga gcaatatcag ggacgtgcaa ggcttgtggg cattttcctt tttgctgtcg
     1141 tgacgggcaa catcgcaggc ctctggatgc atcccgagtc ggtagctatc ggcgcatcgg
     1201 gagggctgtt cggcttaatc ggcatgatgg cggcaatgac aatccgctac taccggcaga
     1261 tgccgcacat gcttcgtaac gcccttaaaa aatggatagc ctcaatactg gtgtataacc
     1321 tgtttttcat gataataccg caagtagaca tatcggctca tgcaggcgga tttgccggcg
     1381 gattcataat gggcctgata gcggcaaggc cgctagggtc gaaagaccgg ccgtcaatcg
     1441 caacactggc tatggcggcg atattgctga tacttacggc taacctggga atgaaagtaa
     1501 tcgccggact gccggcaaaa ctcaaaaaag cagaacctca actcattatc aatctgacgc
     1561 ctgaacagat agattatctg cggcagaata attcgaacgg aatgatagca cctatgccag
     1621 gcaagccggg gcatttttac gtcataaaac tccaccctga atacgcacca tccgttaagc
     1681 ctaaaaaatg atttggtata aaaattattt tcttccgaga gccccaaaca ctgctctcca
     1741 aaaactttca cattacaaaa aaaacgttag acattgacat gccagccagt tatacttctt
     1801 ggcccttgta aaatatttat tgatttaagc ttttttactt aagaggtgag taatgccaaa
     1861 gcccagacgc agacggaaaa taggttccaa aaaacgccgt gaacgccgga atcgccgtaa
     1921 gaaataacct atattttgca ttttgactac gtgaaatcaa aacccggctg gtgtgccatt
     1981 tacgccagcc gggttttagt gcattgaaaa gttgagagtt tcgatttttg cgccagtggg
     2041 ttcgttcgtg gcaaggaagg aaaatgaggc atatttgatg atattcctaa aaaattctga
     2101 cacagccacg aacaaagcaa accagcaaaa aagga
//
LOCUS       DAONFI010000368         2130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_69260_length_2130_cov_3.746024, whole genome shotgun sequence.
ACCESSION   DAONFI010000368 DAONFI010000000
VERSION     DAONFI010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69260_length_2130_cov_3.746024"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..97)
                     /locus_tag="PLK08_09865"
     tRNA            complement(15..97)
                     /locus_tag="PLK08_09865"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Tyr,seq:gta)
     gene            304..1386
                     /locus_tag="PLK08_09870"
     CDS             304..1386
                     /locus_tag="PLK08_09870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPS53846.1"
                     /translation="MVSHRIRIVAATICLLLPCMVLRADTANAASENIIPENTIAQLQ
                     SEPQKMSADMTPQQRQDYIHDFLTKRLAEADAVIAEYPGAVNVSKAMIIKLTACFMLA
                     QQNKLAYEQYSPEILKIADAIVDDKSADQDSKMLAIMYKYRTKIHLTEDKNEIKNMLD
                     EMLGIIKKDYNPAMGFMLAIRMAMEKNNPDLAKAYVTQALVEFPDNESFIRIQKMLDK
                     PAFVGQPFVADVKLLDGKSLKLPADMKGKVVVVDFWASWCGPCRQFAKYLKKFYDKYK
                     DKGVVVLGINMDTDKSAMDAYLKEHSDYTWLQSFSGKGWQDPTAMKYGIEGIPSVWVI
                     GKDGNVVSDNARGDMEGIVDRALSAK"
     gene            1526..1951
                     /locus_tag="PLK08_09875"
     CDS             1526..1951
                     /locus_tag="PLK08_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HPS53847.1"
                     /translation="MSFNRADIFKCTVEGCTAVVDVMEPCCCDTECGLSCCDKVMEKL
                     TPKTADFKTEKHVPVVEKVEGGFKVTVGSTPHPMTDEHYIQMIELRTANRLCRAYLKP
                     GDKPEAFFPIAECCCKSAGCEMPEARELCNIHGLWTNKK"
BASE COUNT          599 a          406 c          574 g          551 t
ORIGIN      
        1 taagatgctg gaattgggag cggatggatt cgaaccatcg aaggcgtctg ccgtcagatt
       61 tacagtctga tccctttgtc cactcgggca cactccctaa acggggatga ataatgtagt
      121 tgatagtgct cctgacgtca agtgaagttt tatgaaatga aacaaaaata attcgaaatc
      181 tggcgatgga agatatccgg gccgctataa ttaaaatatg tcaaaattaa tggatgacat
      241 ttgtgaaatg agaaccagcc ggtaaaatca ttgtttggga aaacttatcg aaggagataa
      301 tttatggtat cgcacagaat tagaattgtg gccgctacga tatgcctgct gttgccgtgt
      361 atggttttac gggcagatac cgccaacgcc gccagtgaaa acatcattcc cgaaaatact
      421 atagctcagc tgcaatccga gccgcagaag atgtctgccg atatgactcc acaacagcga
      481 caggattata tacatgattt tttgaccaag aggcttgccg aggcggatgc ggttatcgct
      541 gaatatcctg gtgcggtgaa cgtttcgaag gcgatgatta taaagttgac ggcatgtttc
      601 atgttggcgc agcagaataa gctggcgtat gaacaatatt cgccggaaat tctcaaaatc
      661 gcggatgcca tagttgacga taaaagcgcc gaccaggatt ccaagatgct tgccatcatg
      721 tacaaatatc gcacgaagat acatcttacg gaggataaga atgaaatcaa gaatatgctt
      781 gacgagatgt tggggataat caaaaaggat tacaatcctg ctatggggtt catgttggcg
      841 atacgcatgg cgatggagaa gaacaatccc gaccttgcga aggcttacgt gacgcaggct
      901 cttgtggaat ttcccgataa cgaaagtttt attcgcatac agaaaatgct tgataagcct
      961 gcgtttgtcg gccagccgtt tgttgcggat gtcaaactcc tggacggcaa atcgttaaaa
     1021 ttgcccgccg acatgaaggg caaggtggtg gtagtggatt tctgggcgtc gtggtgtggg
     1081 ccgtgccggc agtttgctaa gtacctcaag aaattttacg acaaatataa agacaagggt
     1141 gtggtggttc ttggcatcaa catggatacg gataaaagtg cgatggatgc ctatttgaag
     1201 gaacattccg attatacctg gttgcagtca ttcagcggta aggggtggca ggatccgact
     1261 gcgatgaaat atggcattga aggaattccg agcgtatggg taatcggcaa ggacggcaac
     1321 gtagtgtccg acaatgcgag aggtgacatg gaaggtatag tggacagggc gttgagtgcc
     1381 aagtgaattt ctgcgggtta aattgcgtcg taacattgtg tataaataag aaagtttttg
     1441 tctgattatg gcggatgcgt tcacttgtgg ttttaagccg agaaattatg aagggcatat
     1501 aaaaataagg ataaggagag ctaagatgag ttttaacaga gcagacatat tcaagtgcac
     1561 ggtcgaaggc tgcaccgcgg ttgtggatgt gatggagcct tgctgctgtg acaccgagtg
     1621 cggactgtcg tgctgcgaca aagttatgga aaaacttacg ccgaagacgg ctgatttcaa
     1681 gaccgaaaaa catgtaccgg tcgttgaaaa ggtggaaggc ggtttcaagg tcacggtcgg
     1741 ttctacgccg catccgatga ccgatgaaca ctatattcag atgatagagc ttcgtaccgc
     1801 gaatcgttta tgtcgcgcat atcttaaacc tggcgataag cctgaggcgt ttttcccgat
     1861 tgccgaatgc tgctgcaaaa gcgccggctg cgaaatgcct gaagcccgcg aattgtgcaa
     1921 cattcacgga ctctggacga ataaaaaata aaccgccaga cccgctatgg tatgcgtttg
     1981 aaaattcctt gctggtacag gctgttgaat ttgtttgtat ttataaaata gaaccggaat
     2041 gacttttttt atgggtcatt ccgttttttt tttgctgttg tgttttattt tggacgaatc
     2101 agacgatgcc ctgggcaagc atggcttccg
//
LOCUS       DAONFI010000369         2121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_69739_length_2121_cov_3.385770, whole genome shotgun sequence.
ACCESSION   DAONFI010000369 DAONFI010000000
VERSION     DAONFI010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69739_length_2121_cov_3.385770"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLK08_09880"
     CDS             <1..613
                     /locus_tag="PLK08_09880"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HPS53848.1"
                     /translation="DRTTVTIDLQKFNSSLGTLTGITLSLKVTLSNANIQMDNDTESV
                     STATAKVNSQVLSFTSEVTLLKTDYTSFDSINLQVDEAQGFTLQATSGDPTNQFNATG
                     LSDYATWSPGDIESGESGSIASVVWAQYTGSDTFSIDLKSTFGTSASFTGDPCYFQGN
                     TPTAVFSGDVTYTYEPVPEPSTLALLGTLGTIFIIRRYRRKGE"
     gene            complement(701..1165)
                     /locus_tag="PLK08_09885"
     CDS             complement(701..1165)
                     /locus_tag="PLK08_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53849.1"
                     /translation="MSQNDPMPINPDDMEIDDGPQPVPVPIPAPASEDAPEPVPVPVH
                     PKKNEDVEEPIALVDDFDEDSPSQRRAFGSTSTVLQNEFTFKRPLNIDGSGATRCKLF
                     HSKISDGPLSHMQQMINEWLDSEKVEVKFVTQSIGVMEGKHAEPNVIVLMWY"
     gene            1331..>2121
                     /locus_tag="PLK08_09890"
     CDS             1331..>2121
                     /locus_tag="PLK08_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53850.1"
                     /translation="MKRNIIVMLAFAIAVLIWACFRNHPIPPAAHAQGDNSPNAVASR
                     KPSIKNLGKICGISLQIHSGDKTWPTEKLIDEIAATNANTVCLVVHGYQENASSNSIF
                     IDTRKTPSAGLLANSIRHAHMKGLRVMVMPVVLLSKRRGNEWRGQIAPSSWDEWWNDY
                     TAFILYYAQVAQNEGAEFFCVGSELITTETQTYRWARLIADVREVYGGLLTYSANWDH
                     YEVPAFWKDLDLVGMTTYYDLCGISAPTLQTLLESWQKFRKNILT"
BASE COUNT          566 a          557 c          494 g          504 t
ORIGIN      
        1 tgacagaaca acagtcacaa tcgatctgca aaagttcaat tccagcctcg gcacgctgac
       61 tggaattact ctttcgttaa aggtcacatt aagtaacgcc aatattcaga tggataacga
      121 tactgaatcg gtaagcacgg ctaccgcaaa ggtaaacagc caggtattga gttttacatc
      181 ggaagtcact ctgttaaaaa ctgactacac atcttttgat tccataaatc tgcaggttga
      241 cgaagcccaa ggttttaccc ttcaggcgac atcgggcgat ccgacgaatc aattcaatgc
      301 aacagggcta agtgactacg ccacgtggag tcctggcgat atcgaatctg gcgaatcagg
      361 cagcatagcc agcgtggtat gggcacaata tacaggctca gacacattca gcatagacct
      421 taagtccaca tttggcactt cagcgtcctt caccggcgat ccctgctatt tccagggcaa
      481 tacgcctacc gcggttttct caggcgacgt cacttataca tatgaaccag ttcctgaacc
      541 gtcaacattg gcgttattag gaactctcgg cacgattttc atcatacgtc gttatcgccg
      601 caaaggagaa taaagtcacc ttttgaaact gacctgaaca gccattttca aagcagactg
      661 catcgacata tggaaaacgg cgatattttc gcaaaaaacg tcaataccac attaggacaa
      721 tgacgttcgg ctcggcatgc ttgccttcca tgacgccgat gctctgcgta acgaatttca
      781 cctccacttt ttcggagtcc agccattcgt taatcatctg ttgcatatgc gatagcgggc
      841 cgtcggaaat tttggaatga aacagcttgc aacgcgtcgc accgctgccg tcgatattga
      901 gcggccgctt gaatgtgaat tcattctgta aaaccgtgct agtcgaaccg aacgcacgac
      961 gctggctggg agaatcctca tcaaaatcat cgacgagcgc tataggctct tcgacatctt
     1021 cgtttttttt cggatgaacc ggtacgggaa ccggctcagg agcatcctcg ctcgccggag
     1081 ccggaatggg aaccggcaca ggctgcgggc cgtcgtcaat ttccatatcg tccggattaa
     1141 tcggcattgg gtcgttctgc gacatatttt gcactccttt tatagggaac aaaacgcgga
     1201 aacacttccc gcaaaaatcc aaaaatcctc gcttcaggtt ttaacggcat catgcaaaat
     1261 ccaaaatgga ttttttcagc cgtttccatt ataattcttc gatattcaac gtcaagtaat
     1321 tggaaaaatt atgaagcgta atattatagt catgctggcg ttcgccattg ccgtgttgat
     1381 atgggcctgt ttcaggaatc acccaatacc gcctgctgcc catgcgcagg gcgataactc
     1441 accaaacgcc gtggcgtcaa gaaagccgtc gattaagaat ctgggcaaaa tatgcggcat
     1501 aagccttcag atacattccg gcgataaaac atggccgacg gaaaaactca tagacgaaat
     1561 cgccgccaca aacgcaaaca ccgtctgcct ggtggttcac ggatatcagg agaatgcgtc
     1621 gtccaacagt atttttatag acacgcgcaa gactccgtcg gctggcctgc tggcaaattc
     1681 catccgccac gcgcatatga aaggtctgcg ggtcatggtc atgcccgtgg tgcttctctc
     1741 caagcgtcgc ggcaacgaat ggcgcggcca gatagcccca tccagttggg acgaatggtg
     1801 gaacgattac accgcgttca tactctacta cgcgcaagta gcgcagaacg aaggcgccga
     1861 atttttctgc gtcggctcgg aacttatcac aacggaaacg caaacctacc gctgggccag
     1921 gctcatagcc gacgttcgtg aagtttacgg cggccttctg acatattcag ccaactggga
     1981 tcattatgaa gtgcccgcgt tctggaaaga cctcgacctc gtgggcatga ccacgtacta
     2041 cgatttatgc ggcatatcgg ccccgactct tcagacgctg ttggagtcgt ggcaaaaatt
     2101 ccgtaaaaat atccttacgt g
//
LOCUS       DAONFI010000370         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_70409_length_2108_cov_4.730638, whole genome shotgun sequence.
ACCESSION   DAONFI010000370 DAONFI010000000
VERSION     DAONFI010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70409_length_2108_cov_4.730638"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            719..2053
                     /gene="rho"
                     /locus_tag="PLK08_09895"
     CDS             719..2053
                     /gene="rho"
                     /locus_tag="PLK08_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPS53851.1"
                     /translation="MVTEESSQSHVETPEELNAKYEEIKRGALHINQLQKMAVPELHE
                     LARKEGITDHAAMNRKELVFHIIKARIMQNGMTFGEGVLEILPDGFGFLRSSDYDFLP
                     SPDDIYVSPSQIRRFGLKQGNIVSGQIRPPKDIEKYFALLRVEAVNHESPDRLDNQTG
                     FEELTPMFPERRIFLETDPKDLNTRVVNLVTPIGFGQRMLIVAQPRTGKTILMKKIAN
                     AITTNHPDAHVVILLIDERPEEVTDMRRNTKAEVISSTFDEPSTRHIQIADMVLAKAK
                     RMVEFGKDVVILLDSITRLARAYNANAPHSGKILTGGVDASALQEPKRFFGAARAVEE
                     GGSLTVIGTALIDTGSRMDEVIFEEFKGTGNSELHLDRRLSDSRIYPAIDINRSGTRK
                     EELLLDPTENKLVQRMRRVLADMQPAEAMELLLTRLAKTSSNAEFLLSMKID"
BASE COUNT          569 a          577 c          545 g          417 t
ORIGIN      
        1 gtgatattcc taaaaaaatt ctgacgcagc cacgaacaaa gcaaaccagc aaaaaatgaa
       61 ttatcaaggt taaaatgctc tggaggcctg catgccgtca aatgcttaca tggtatactt
      121 gcgataaata cgtattatta tatttttcag gagattgtcg aacatggcca aagctgccaa
      181 taaaacaagt gctgcgcccg aaaagaaggc tgtcagaaag aagtcgactg cccaatccgg
      241 cccggcgaaa gccgtaaaaa aaacgtccga gcaaaaggct atgaccggca gccttttcgg
      301 agagatgaat gaatcgtcgg cagttggcat ccaagccgtg gaagaaatgg ctgatgccgt
      361 cgcacaggtt cgccaggcaa tgcaggctga aacggcaggt cagcctttgg ctactgcgac
      421 tcagccggaa gagaaaccag ccaaaaaacg caggggccgc cctcctaaaa gcgccaaatc
      481 ccccgattct gctccgcagg aagcgttgtt gccgcaagac gctccgacgg cagtcgaagt
      541 gcctcagatt caagccgatt cccagcctgc tgaagaaaag cctgcccaaa aacgcagagg
      601 ccgtccgccg aaaaataaac agacgccgcc gctgaccacc ccttcccaaa catcgtctgc
      661 cgacgaggaa accagccccg taaccgtagc ggacgaaaaa caggctgccg atgacgttat
      721 agttacggag gaatcgtcgc aatcgcatgt tgaaacgccc gaagaactca acgccaagta
      781 tgaggaaatc aaacggggcg ctttacatat aaatcagtta cagaagatgg ctgtgccgga
      841 attgcatgaa ctggctcgca aagagggcat taccgaccat gcagccatga atcgcaagga
      901 actggtgttc catataatca aggcacgcat aatgcagaac ggcatgacgt tcggcgaggg
      961 cgtgctggag attctgccgg acgggttcgg tttcctgcga agctccgatt acgattttct
     1021 gcccagtccg gatgacattt acgtttcgcc gagtcagatt cgtcgtttcg gcctcaaaca
     1081 gggcaatata gtctctggcc agattcggcc gccgaaagac atcgaaaaat attttgccct
     1141 gcttcgcgtt gaagccgtca atcacgaatc ccccgacagg ctggataacc agacgggctt
     1201 tgaagaactt acgccgatgt ttcctgaaag acgtattttt ctggaaaccg accccaaaga
     1261 cctcaatacg cgggtggtca atctggttac gcctatcggc tttggccaac gtatgttaat
     1321 cgtggctcag ccgcgcacgg gcaaaaccat acttatgaaa aaaatcgcca acgcgataac
     1381 gactaaccat ccggacgccc atgttgtaat tctgctgata gacgaacggc ctgaggaagt
     1441 aaccgacatg cgtcgcaaca ccaaagccga ggtcatctcc agtacgttcg atgagccgtc
     1501 cactcgtcac attcagattg ccgacatggt tctcgcaaaa gccaagcgca tggtggaatt
     1561 cggcaaggat gtcgtgattc tgctggacag cattacgcgt ctggcacgtg cgtacaacgc
     1621 caacgcgccg cattccggca agattcttac cggcggcgtc gatgcttctg cattgcagga
     1681 gcccaagagg tttttcggcg cggctcgcgc ggttgaggag ggcggctcgc ttacggttat
     1741 cggcacggca ctcatcgaca ccggcagtcg catggacgag gttatttttg aagaattcaa
     1801 aggcaccggc aacagtgagc ttcaccttga ccgccgcctg agcgacagcc gcatttatcc
     1861 ggctatcgac atcaaccgct ccggcacgcg aaaggaagaa ctgctgctcg acccgactga
     1921 aaacaagctg gttcagcgca tgcgtagagt gctggctgac atgcagccgg ctgaggcgat
     1981 ggaacttctc ctgacgcgac tggctaaaac ttccagcaat gccgaattcc tgctgtccat
     2041 gaagatagac taaaacctat ccccaaaacc ttggctgcct gcaatcggct cttttgcagc
     2101 ctggcggc
//
LOCUS       DAONFI010000371         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_71556_length_2087_cov_4.361713, whole genome shotgun sequence.
ACCESSION   DAONFI010000371 DAONFI010000000
VERSION     DAONFI010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71556_length_2087_cov_4.361713"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1046
                     /locus_tag="PLK08_09900"
     CDS             279..1046
                     /locus_tag="PLK08_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53852.1"
                     /translation="MEIFGRLLMLLVVCVLAAGCNRTSRKVYDETPGEQQTSGVGKSI
                     YSTETVDVDAVYEIKEPQTAVAPKPEVKPKPEPAKPAKKTPAPAAPKPLAAKPAPTTA
                     PVAASPTTKPATAPKTKTAAKPVTAAKKIAAKPVAKKTVTPESTVAAATAVEPPAPVK
                     APSAAAPTDADKTKKKTIDAATAKNIEKAKKQIQKKVGPITPEMLQKIQQLVVEGKIQ
                     QADMAKVAAIKLKVTSGKALNKQDLQYLDALKKKYKL"
     gene            1065..>2087
                     /locus_tag="PLK08_09905"
     CDS             1065..>2087
                     /locus_tag="PLK08_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPS53853.1"
                     /translation="MADTRENWGSRAGFVLAAIGSAVGLGNLWGFPYKLYKTGGGAFL
                     IPYVIAMLFVGIPLLIAEFSLGHITQRSTPSAFARVKRPYAVVGWWQIILSFIIITYY
                     AVILAWCGSFLVYSIEGIFQGTLPWAGVGVEGVKNASEFFYGKYLGHTSGFALGKMQL
                     HVVVALFITWLAMYLCIFRGVKLVSKIVLWTVPLPWLMLLILTIRGLTLPGATQGMEY
                     YLEPNFSQLADPQTWRLAFGQMFFSMSLAFGVMITYSSFLHRKSDLNNNAAIIGLADM
                     GTSFVAGIAVFATLGGMAFATQQAGNPIPVDRIADGGPGLAFVAFPYALAQLPYAPYF
                     SVVFFLA"
BASE COUNT          515 a          511 c          566 g          495 t
ORIGIN      
        1 attcggaccc ggagccaatt ggcgcatact tgtgggccga tataactaca gcgtttatat
       61 cgacaggcag ggcccggagc tctcgtcgtt tccgaaattg ggcaagccga attatcatct
      121 ttgcgacgaa tacggcagag tggtatttgt gaagtgacga cgcagcctgc ggaattgatt
      181 tgcataaacc gccgtgaaag gctaaaaaaa tatggaagat tttcaacaaa aacgggttcg
      241 gcctaaaaat aagtttaatg tgtgcgagga aaattgagat ggaaatattt ggcagattat
      301 taatgttgct tgtggtatgc gttctggcag ccggctgtaa caggacgtcg cgaaaggttt
      361 acgacgaaac gcctggcgaa cagcagacgt caggtgtcgg caaaagcata tattcgactg
      421 aaactgtgga tgtcgacgct gtttatgaaa taaaagagcc gcaaaccgcc gtcgctccca
      481 agcctgaagt aaaacccaaa cctgaaccgg ccaagcctgc gaaaaagact cccgctcctg
      541 ctgcgcccaa gccgttagcc gccaaaccgg ctcccacgac agctcctgtc gcagcatccc
      601 cgacaaccaa acctgcaact gcaccgaaaa ctaaaacggc tgccaaacct gttactgctg
      661 caaagaaaat tgcggccaaa cccgtggcca aaaaaacagt tacacctgaa tcgactgttg
      721 ccgccgcaac tgcggtagag ccgccggcgc cggtaaaagc accatctgcc gccgctccga
      781 cagatgccga caagacaaag aaaaaaacca tagatgccgc cacggctaaa aatatcgaaa
      841 aggctaaaaa gcagatacag aaaaaagtcg gcccgataac gcctgaaatg ttgcagaaga
      901 tacagcagct cgtggttgaa ggcaaaattc agcaggcgga tatggccaaa gttgctgcca
      961 tcaagcttaa agtgacgtca ggcaaggcgt tgaacaagca ggacttgcaa taccttgatg
     1021 cgttgaagaa aaaatataaa ctttgatgat ttttgggaga aacaatggcg gatacccgtg
     1081 agaattgggg ttcgagagcc ggtttcgttc tggcagctat tggctcggcg gtagggcttg
     1141 gcaacctgtg gggttttcct tacaaacttt acaagaccgg cggcggtgct tttctcatac
     1201 cgtacgtcat tgcgatgctt tttgtgggca taccgttgct catcgcggaa ttttcacttg
     1261 gccatattac gcagcgttcc acgcccagtg cgttcgcccg cgtcaaaagg ccatacgccg
     1321 tcgtcggctg gtggcagata atactcagtt tcatcatcat aacgtattat gcggtcatac
     1381 tggcgtggtg cggaagtttt ttggtttatt cgatagaggg aatttttcag ggcacgctgc
     1441 cgtgggccgg cgttggcgta gagggcgtca aaaatgccag cgaatttttt tacggcaaat
     1501 atctcggcca tacttcggga tttgccctgg gcaaaatgca gttgcacgtc gtcgtggcgc
     1561 tgttcattac atggctggct atgtacctgt gcattttccg cggcgtcaag ctggtcagca
     1621 agatagtttt gtggactgtg ccgctgccgt ggttgatgct gcttattctt actattcgtg
     1681 gccttactct gcccggcgct acgcagggca tggaatatta tcttgagccg aatttttcgc
     1741 agcttgccga cccgcagacg tggcgtctgg cgttcggcca gatgtttttc agcatgtcgc
     1801 ttgccttcgg agtgatgata acgtattcga gctttctgca tcgcaagagc gacctcaata
     1861 acaatgccgc cataatcggc ttggctgata tgggtacgag tttcgtcgcc ggcatagcgg
     1921 tttttgcaac gctcggcggc atggcatttg cgacgcagca ggctggcaat ccgataccgg
     1981 tggacaggat tgccgatggc gggccggggc tggcattcgt ggcgtttccg tatgcgctgg
     2041 cacagctgcc gtatgcccca tatttctcgg tggtattttt tcttgcg
//
LOCUS       DAONFI010000372         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_71814_length_2082_cov_3.560434, whole genome shotgun sequence.
ACCESSION   DAONFI010000372 DAONFI010000000
VERSION     DAONFI010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71814_length_2082_cov_3.560434"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..303)
                     /locus_tag="PLK08_09910"
     CDS             complement(1..303)
                     /locus_tag="PLK08_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53854.1"
                     /translation="MSQYSSKDSSSGKCAYKDGVHEAFDVNGHDNDHVPARDVLKLLD
                     KELAAKLTAALYSSPLIRKDLVNRVKMEILAGIYETPERIEGTIDRLLEEFNDNWL"
     gene            complement(688..>2082)
                     /locus_tag="PLK08_09915"
     CDS             complement(688..>2082)
                     /locus_tag="PLK08_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HPS53855.1"
                     /translation="KNPQYRDIPSRVKDYFEIPLPLSEEDILIQAARCMDCGVPFCHA
                     VGCPLHNRIPEFNDLVYSGRWKEACDVLHSTNNFPEITGRICPALCEAACTINIAGEN
                     AEPVTIRHIECQVAERGWKEGWIVPQPAEKKSGRKVAIVGSGPAGLAAAQQLARCGHD
                     VVVFEKDNNVGGLLRYGIPNFKLEKEILDRRVKQLEAEGVEFRTGVTVGEDISMGYIQ
                     KFSDAILLTMGAGEPRDLAVDGRGLENVHFALEYLSQQIRRMENSLDKDEKLISANGK
                     NVIVIGGGDTGSDCVGTARRQGAKSIWQLEILPKPPADYNPETPWPNYPRILRTSTSH
                     EEGCERRWSVTTKKLVGSGVKVNKIICCEVDWINKNGKWEMKEIPNSTFELDADLVLL
                     AMGFLHVAHKGVVDAFGLKTDSRGNLAINNYQTSRAGIFAAGDTTRGASLVVWAIQEG
                     RNAAEAIDSYLKSL"
BASE COUNT          436 a          553 c          529 g          564 t
ORIGIN      
        1 tcataaccag ttgtcgttga attcttccaa aaggcggtcg attgtgcctt cgatgcgttc
       61 cggcgtctcg taaatgccgg cgagtatctc catcttgacg cgattgacca ggtcttttcg
      121 gataagtggc gaactgtata aagcggcggt tagtttggcg gctaattcct tgtcgagaag
      181 tttgagaaca tctctggcgg gaacatggtc attatcgtgg ccgtttacat cgaaggcttc
      241 atgaacaccg tctttatacg cacatttccc ggacgacgaa tccttagaag agtattggga
      301 catcctgata ttgctcctga cagcttacac agccgccgct ggggcaagtg gcgaactccc
      361 attcatccac tgctgagtct tttctgatga taccgttcct ggtgccggcc acacattcct
      421 ttggcgaacg acatcgaaac ctcgctggta tatatatcgg aattttcggg aagcgacatt
      481 agaagatttt tcactgcaac gattcccaat gtagccgcaa atgccgggca tgatatttgg
      541 tgtcattcca agcgtagcgg ggaaatcgaa ccgtcataag tcattgcgat tcaggcactt
      601 tgaaatgtct ggcggttata atgcggcacg cagcaatttt tcacaaaact caatggtttt
      661 tgaacgcggt tttcaaaata ttttctttca gaggcttttg agatacgagt ctatagcctc
      721 cgcggcattg cggccttcct gtatagccca aacaaccagg cttgcaccgc gagtggtatc
      781 gccggcggca aatatgccgg ctctcgatgt ctgataatta tttatcgcca ggttgccgcg
      841 gctgtcggtt ttaaggccaa acgcatctac tacgcctttg tgcgctacat gcaggaaacc
      901 catcgccaac aggacaaggt cggcgtccag ctcaaacgtg ctgttcggga tttccttcat
      961 ttcccatttg ccgtttttgt taatccaatc gacttcgcag cagatgattt tgttcacctt
     1021 tacgccgctg ccgacgagtt ttttagtcgt taccgaccac cgccgttcac agccttcttc
     1081 gtgtgaagtg ctggtacgaa gtattcgcgg gtaattgggc cacggcgttt caggattgta
     1141 gtcagccggc ggtttgggca gtatttcaag ctgccagatg ctttttgcgc cctgccgtcg
     1201 tgctgtgccg acgcagtcgc tgcccgtgtc gccgccgcct atgacaatga cgtttttgcc
     1261 gttggctgag atgagttttt catccttatc cagagaattt tccattcgcc gaatctgttg
     1321 cgagagatat tcgagtgcga agtgaacatt ttccaggcct ctgccgtcta ccgccaggtc
     1381 gcgcggttcg cctgcgccca tcgtcagcag gatggcgtcg gagaattttt ggatatatcc
     1441 catggaaata tcttcgccga cggtgacgcc cgtgcggaat tccacacctt cggcttcaag
     1501 ctgttttacg cggcggtcga ggatttcctt ttccagcttg aaattcggaa tgccgtatcg
     1561 cagcaagccg ccgacgttgt tatctttttc aaaaaccacc acatcgtggc cgcaacgggc
     1621 aagctgttgt gcagcggcta aaccagccgg gccggagccc actatcgcca ctttacggcc
     1681 gctctttttc tccgcaggtt gcggcactat ccagccttct ttccagcccc gctcggcaac
     1741 ctgacattca atgtgacgaa tggttaccgg ttcggcgttt tcgccggcga tgttgatggt
     1801 gcatgccgcc tcgcaaagtg ctggacagat tctgccggta atttcgggaa aattgttagt
     1861 cgaatgcagg acgtcgcacg cttccttcca gcggccgctg tataccagat cgttgaattc
     1921 cggtatgcga ttatgcagcg gacagccgac cgcgtggcag aacggcacgc cgcaatccat
     1981 acagcgagca gcctgtatca gtatatcttc ctcgctcagc ggaagcggta tttcaaaata
     2041 atccttaact cgcgacggga tgtcacgata ttgcggattt tt
//
LOCUS       DAONFI010000373         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_72959_length_2062_cov_3.715496, whole genome shotgun sequence.
ACCESSION   DAONFI010000373 DAONFI010000000
VERSION     DAONFI010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72959_length_2062_cov_3.715496"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="guaB"
                     /locus_tag="PLK08_09920"
     CDS             complement(<1..1152)
                     /gene="guaB"
                     /locus_tag="PLK08_09920"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPS53856.1"
                     /translation="MKDKIIGDGITFDDVLLIPARSSVHPSEIDTSTRVTRNIRINIP
                     LVSAPMDTVTESALAIALAQEGGLGIIHRNLSIEMQALEVEKVKRSENGILTDPITLP
                     PHESTSVVREAMNKNKVSGFPIVEKDGTLVGILTQRDMKFLTEDTEISKVMTKTNLVT
                     APPNTTLDEAEKILSRAKVEKLLLVENGKLKGMITMADIEKTRNHPQSCRDERGRLRV
                     GAAVGVMDYERIAALIEKDVDIICVDTSHGHKEIVMQTVREIKAKWDIEVIAGNIATA
                     AAARDLIEAGADGLRIGIGPGAICTTRVVTGVGVPQITAVSEAARVADAFDVPVIADG
                     GIRHSGDITKALAAGASCAMMGSLFAGMEESPGDLVLFRGRRYKGYRGMG"
     gene            complement(1354..1869)
                     /locus_tag="PLK08_09925"
     CDS             complement(1354..1869)
                     /locus_tag="PLK08_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPS53857.1"
                     /translation="MDILIACGLLIVNVICLAITAMTFPGNWMMVTIACLVAWWRWDE
                     HFFGIPVLVLIVLLAALGEVLEFATSAVHVKKAGGSKSGSWGSLIGAVIGLTAGTFLI
                     PVPVLGTLIGACGGAFLGAMLCEKHIAGKDIRSATGSGWAAAKGRFMGTVYKFICGVI
                     IYFVIGVAAFA"
     gene            1862..>2062
                     /locus_tag="PLK08_09930"
     CDS             1862..>2062
                     /locus_tag="PLK08_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53858.1"
                     /translation="MSIFTSNQLRAVSRRIIVVEPIPKTLAACNRLFCSLAASEKRQN
                     ILKYVAIFLPCQPAKLLDFGDAH"
BASE COUNT          450 a          566 c          504 g          542 t
ORIGIN      
        1 acccatgccg cgatatccct tgtaacgtcg gcctctgaat agtaccaggt cgccgggcga
       61 ttcttccatg ccggcgaaca ggctgcccat catggcacat gacgcgccgg cggcgagagc
      121 ctttgtgata tcgccgctgt gacgtatgcc gccgtcggct atcacaggca cgtcgaacgc
      181 atctgcgacg cgggcagcct cagaaacggc tgttatctgc ggcacgccta cgccggttac
      241 cacccgcgta gtgcatattg cgccagggcc tatgccgatg cgtaagccgt cggcgccggc
      301 ttctatgagg tcacgtgctg cggctgcggt agcgatattg ccggcgatta cctctatgtc
      361 ccacttagcc ttgatttccc gaaccgtctg catcacgatt tccttgtggc cgtgggatgt
      421 atcgacgcaa ataatatcga cgtctttttc tatcagtgcc gctatacgtt cgtaatccat
      481 gacgccgacg gctgcgccta ctcggagacg gccgcgctcg tcgcggcagc tctgcgggtg
      541 attgcgggtt ttttcaatgt cagccatcgt tatcatgccc ttgagcttgc cgttctcaac
      601 gagcagaagt ttttccacct tcgctctgga gagtatcttt tccgcttcgt ccagcgtggt
      661 gttcggcggt gcggtaacga ggttggtctt cgtcatcacc tttgaaattt cagtatcttc
      721 cgtcaaaaac ttcatatccc gctgggtgag tatgcccacc agtgtgccgt ctttttcgac
      781 tataggaaaa cctgaaacct tgtttttatt catcgcttcg cggacgacgg atgtggattc
      841 atgcggcggc agagttatcg ggtcggtcaa tatcccgttt tcagaacgtt tgactttttc
      901 aacctccagc gcctgcattt ctatgctgag gttgcggtga attattccaa gtccgccttc
      961 ctgggccaac gcaatagcca gtgcggactc cgttaccgta tccatcgggg cggataccag
     1021 cggtatgttt atgcgaatat ttcgcgttac cctggtggat gtgtcaatct ccgagggatg
     1081 aacgctcgaa cgcgccggaa tcagcagcac gtcgtcaaac gttatcccgt cgccgattat
     1141 cttgtctttc atgtttactc cgcatttttt atgtggatat acttcaaccg tgattggcag
     1201 ctaattttac atatttccag cctgcaatgt aaggctggag ttgacaagac gtaaaataaa
     1261 ttttaatttt tccgatgtgg cggattcgta tcatcagttt cgataaagag ttattctgca
     1321 tatattattg aaaagcatga aaagggggtc tttttatgcg aaagccgcca cacctatgac
     1381 gaaatatata atcactccgc atatgaactt gtaaacagtg cccatgaatc ttcccttggc
     1441 ggcagcccag ccgctgcccg ttgcgctgcg gatgtccttg ccggctatat gcttttcgca
     1501 gagcattgct cctaaaaacg caccgccgca tgcgcctata agcgtgccga gaacgggaac
     1561 aggtatcaga aaagtgccag ccgtcaagcc tatgaccgca ccgattagtg aaccccagct
     1621 tccggatttg ctgccgccgg cttttttgac gtgtactgcg gaagttgcaa attcaagcac
     1681 ctcgcccaat gccgccagaa gcactatcaa aaccagcacg ggtatgccga aaaagtgctc
     1741 atcccaccgc caccatgcaa cgaggcaggc gatggttacc atcatccagt tgcctggaaa
     1801 cgtcatcgcc gttatcgcca ggcatatgac attcactatc agcagtccgc aggcaattaa
     1861 aatgtccatt tttacctcga atcagctcag ggctgtaagt cggagaatta ttgttgtaga
     1921 acctatccca aaaaccttgg ctgcctgcaa tcggctcttt tgcagtctgg cggcgtcaga
     1981 aaaacggcaa aatatcctta aatatgttgc catttttctt ccttgccagc cagcaaaatt
     2041 gctcgatttc ggcgacgccc at
//
LOCUS       DAONFI010000374         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_73749_length_2048_cov_4.439538, whole genome shotgun sequence.
ACCESSION   DAONFI010000374 DAONFI010000000
VERSION     DAONFI010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73749_length_2048_cov_4.439538"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1711)
                     /gene="ilvB"
                     /locus_tag="PLK08_09935"
     CDS             complement(14..1711)
                     /gene="ilvB"
                     /locus_tag="PLK08_09935"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPS53859.1"
                     /translation="MTRKTKYKGLNGAQLLNQILIEQGVTTLFGYPGGVLLPIFDVLY
                     DSPLKFVLTRHEQGACHMADGYARSSGKVGVVIATSGPGATNLVTGLATANMDSVPIV
                     AITGQVKSDLIGNDAFQEADLTGITRPITKHNVLVKDVRDLGRVLNEAFYIANSGRPG
                     PVLVDIPVDITTAILEEEPDVEPRLPGYKPRGGEGNERQAKLAAKTINAAKKPVLYVG
                     GGVILSGASDVLREMAEKGRLPVTTTLLGLGAIDETSELSLKMLGMHGTAYANHAVQN
                     CDCLVAIGARFDDRVTGDVKRFAPHAKIIHIDIDPSSISKNVQVDIPLVGDARSIIEQ
                     MLPHIESVDRHEWLAQIADWKKRFPLKYKSTGAIKPQEVIDMLGEKTGHDAIIATGVG
                     QHQMWTSQYYGWRKPRQILTSGGLGTMGYGVPAAIGAQFAFPDRQVIDIDGDGSFTMT
                     MVEVITSVQYGLKTKFIVLDNEYLGMVRQWQQLFYHKRYSSVSHPCPDFAMIARGYGA
                     EAATVRQREELPAAMDKMLASDKTFVLHIQVAKEENVYPMVAAGKALDDMIHSEELI"
     gene            1832..>2048
                     /locus_tag="PLK08_09940"
     CDS             1832..>2048
                     /locus_tag="PLK08_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53860.1"
                     /translation="MKFSAYNGLTNGNHAQSRHGYDAASYPYNAQKHHIVSHAGIHDA
                     DMLHPAIENAIFFFKNGSSRPIYTIDLI"
BASE COUNT          434 a          587 c          542 g          485 t
ORIGIN      
        1 ttatcgtcag tcatcatatg agttcctcgc tgtgaatcat gtcgtccaac gccttgcctg
       61 cggcaaccat cggatagacg ttttcttcct tagccacctg gatgtgcagc acgaacgttt
      121 tgtccgacgc aagcatttta tccatagcgg ctggcagttc ttcccgctgc cggacagtag
      181 cagcctccgc accgtaaccg cgagctatca tggcaaagtc gggacacgga tggctgaccg
      241 aagaataacg tttgtggtaa aacagctgct gccactggcg aaccatgccg agatattcgt
      301 tgtccaggac gataaacttc gtcttcaggc cgtactggac ggaggttatg acttcaacca
      361 tcgtcatggt aaaactgccg tcgccgtcga tatcgattac ctgcctgtcc gggaacgcaa
      421 attgcgctcc gatggcagcc ggcacgccgt aacccatcgt ccccaagccg ccggacgtca
      481 gaatctgcct cggcttgcgc cagccataat attgcgaagt ccacatctga tgctggccga
      541 cgccggtggc aattatggca tcgtggccgg tcttttcacc aagcatgtcg atgacttcct
      601 gcggttttat cgcgccggtg gacttatact taagcgggaa gcgttttttc cagtcggcaa
      661 tctgcgccag ccattcatgc ctgtcgacac tttctatatg cggcagcatc tgctcgatta
      721 tcgacctggc gtcaccgacg agcggtatgt caacctgaac gtttttggat atcgaagacg
      781 ggtcgatatc tatatggatt atcttcgcat gcggggcgaa ccgtttgaca tcgccggtaa
      841 cgcggtcgtc gaaacgcgcg ccgattgcta ccaggcagtc gcaattctgc acggcgtgat
      901 ttgcatacgc agttccgtgc atacccagca ttttcaggct gagttcgctg gtttcgtcga
      961 tagcgccaag gcccagcagc gtcgtggtga cgggcagtct gcccttttcc gccatttcgc
     1021 gcaggacgtc gctggcgccg gagagtatga ctccgccgcc gacgtacagc acgggctttt
     1081 tcgccgcatt tatcgtctta gccgcaagtt tggcctgccg ttcattgcct tcgccgccgc
     1141 gaggcttgta acccggcagc cgcggctcaa cgtccggctc ttcttccaga atcgctgtag
     1201 tgatgtcgac aggtatatcc acaaggaccg ggccgggccg gccggaattg gcgatgtaaa
     1261 acgcctcgtt caacacgcgg ccgaggtcgc gaacatcctt gacgagaacg ttatgcttcg
     1321 taatcggccg ggttatgccg gtgaggtcgg cttcctggaa cgcatcgttg ccgatgagat
     1381 cgctcttgac ctggccggtt atggcgacta tcggcacgga gtccatattc gccgtcgcaa
     1441 ggccggtgac caggttcgtc gcaccaggcc ctgaggtggc gataaccacg ccgactttgc
     1501 cgctcgacct cgcatacccg tcagccatgt ggcatgcgcc ctgttcgtga cgggtaagaa
     1561 cgaattttaa gggcgagtcg tacagcacgt cgaatatcgg aagcagcacg ccgcccgggt
     1621 aaccgaacag ggttgtgacg ccctgctcaa tcaatatctg gttgagcagc tgtgctccgt
     1681 taaggccttt gtatttcgtt ttcctggtca tattataaat cctcagtcta aaacttgaaa
     1741 gtcatccgaa tcagatgaat atacggaaat tccgccggaa atcaaagaga gttttcgaag
     1801 gattgccgtc acgccggttc ggccgccgca ggtgaaattt tcagcctaca acggcctgac
     1861 taacggtaac cacgcgcaga gccggcacgg atacgacgct gcgtcctacc cttacaacgc
     1921 acaaaagcac catattgtgt cccatgccgg tattcatgat gccgatatgt tacatccagc
     1981 cattgaaaat gcaatctttt ttttcaaaaa cggctcaagc cggccgattt atacaataga
     2041 cctgattt
//
LOCUS       DAONFI010000375         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_74243_length_2039_cov_7.549395, whole genome shotgun sequence.
ACCESSION   DAONFI010000375 DAONFI010000000
VERSION     DAONFI010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74243_length_2039_cov_7.549395"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLK08_09945"
     CDS             complement(<1..486)
                     /locus_tag="PLK08_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPS53861.1"
                     /translation="MTYMNYILNLLKPHRKRIVLASAALLMLMLADLGTPIIIALLID
                     DVIGQSQHALLVPLMIFFLILPLYASVMRFISNYIIAIISQRIILDLRLDLYRHIQRL
                     NCNYLHSTTTGRIMERMRGDVIKLQNVLTSQTLQLGVQVVTGLIMVVTMLAISVKMTL
                     IV"
     gene            complement(1289..>2039)
                     /gene="argG"
                     /locus_tag="PLK08_09950"
     CDS             complement(1289..>2039)
                     /gene="argG"
                     /locus_tag="PLK08_09950"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPS53862.1"
                     /translation="LTSREAAIEYAKAHNIPIHQSKKKIYSRDRNLWHISHEGADLED
                     PANEPKDDLFVMSVPLSKAPAKPEYVEIEFLKGIPVAVNGKKMQGHDLIAMLNEIGGR
                     HAIGQEDLVENRLVGIKSRGVYETPGGEILYRAHQALETITLDKDTLHFKQKIALEYA
                     QLVYDGKWFCPLRESLDVFIENTQRKVTGKVKIKCFRGRPTLAGVVSPNSLYSHNLAS
                     FTMGEEYTPSDATGFIKLFGLQMKGRGGKIK"
BASE COUNT          522 a          459 c          498 g          560 t
ORIGIN      
        1 cactatcaat gtcattttga cgcttatcgc cagcatcgtg acgaccatga tgaggccggt
       61 caccacctgc acgcccagtt gcagagtctg ggaagtcaac acgttctgaa gtttgataac
      121 gtcgccgcgc atgcgttcca tgattctgcc ggtcgtggtg gaatgcagat aattgcagtt
      181 cagccgttga atatggcgat acagatcaag acgcaggtcg agaattattc tctgcgaaat
      241 tatggctatt atatagttgc ttataaagcg cattacgctg gcatacagag gcagtatgag
      301 gaaaaatatc atcaggggca caagcagggc gtgttgactc tggccgatga catcatcgat
      361 aagcagggcg atgataatcg gagtgcccaa atcagccagc atcagcataa gcaacgccgc
      421 actggcgaga actattcttt tgcgatgtgg tttcaggaga ttgaggatgt aattcatgta
      481 tgtcatgact tacaggcacg cacaacgtga gacgtttcgg ttcaaaacag gatttgctgt
      541 gtcagattga agaaaaccag ctggaaatgc actgttccca ttattgctga gaggggattg
      601 tacccggagc actgagagga aaagttgcag acagcacacg taagagactc cagggaacaa
      661 tttaacggct gatgttcaat ccgcttaacc ctgaaaagtt gtgcctatgc caggctcctt
      721 aaaggagacg taaaatacaa ccacatgaat gagccgccat ttcatgtctt tcgcatatcc
      781 ggctaagccg cctcaaactt ggccagtatt gcgaaatatc ctacaaaaac ggggattata
      841 tcaccatatt atgactatac aagatattaa taacattttt tgacggaaaa ttcttcctgt
      901 caaatgtcac attatagcac acatccgaca caattatatc gtcattccgt taattcttga
      961 ttaaaagttt tttttagagc atcttaaagg tttcagaatt tttttagagc atgttaaatt
     1021 tgaaattgcg ccttgttgcg tcgttgagga cgaggctggc aaggaaggcg aagcaggcaa
     1081 ccttatatgg tttccgatgc agcctgacgt agccagccga cgccgcaaac agcaacaagc
     1141 gcaaagtcaa gtttaatatg ctctaaaggt gtataaaagt atatggcgaa gttccccaaa
     1201 gaagtctttt ctattaagaa atgttataaa aaacgcccgt atgtcgtatt acgggcgttt
     1261 tcgtttctct aagttgatta tagagcgatt attttatttt gccgccgcgg cctttcatct
     1321 gcaaaccgaa gagttttata aatccggtgg catcggacgg cgtatattct tcgcccatgg
     1381 taaagctggc cagattgtgc gaatacaggc tgtttggcga tacgacgcca gccagcgtcg
     1441 gccggcccct gaaacatttt atctttacct tgccggtgac tttgcgctgc gtattttcaa
     1501 taaaaacgtc aagcgactcg cgcagcgggc agaaccattt gccgtcatag acgagttgcg
     1561 cgtattcgag ggcgattttc tgtttgaaat gcagcgtatc cttgtcgagc gtgatagtct
     1621 ccagggcctg atgggcacga tagagtattt cgccgcccgg tgtttcatat acgccgcggg
     1681 attttatgcc gactagccgg ttttccacca gatcttcctg accgatggca tgtcggccgc
     1741 cgatttcgtt gagcatcgct atcaaatcat ggccttgcat ctttttgcca tttacggcta
     1801 cgggtatgcc cttgagaaat tcgatttcga cgtattccgg tttggcaggt gctttggaca
     1861 gcggcacgct catcacgaac aggtcatctt tgggttcgtt cgccgggtct tccaggtcgg
     1921 cgccttcgtg cgagatatgc cacaggttgc ggtctcgcga atagattttc tttttgctct
     1981 gatgaatcgg tatattatga gccttcgcgt attcgatagc tgcttcacgg ctggtcagt
//
LOCUS       DAONFI010000376         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_74252_length_2039_cov_3.926915, whole genome shotgun sequence.
ACCESSION   DAONFI010000376 DAONFI010000000
VERSION     DAONFI010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74252_length_2039_cov_3.926915"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PLK08_09955"
     CDS             complement(<1..1198)
                     /locus_tag="PLK08_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPS53863.1"
                     /translation="MRSMKYVLPYKLNIVAAFLLVVMIACLWGGGIGMALPALKILLS
                     EEGLHGWAWDSISEDKLMAKLTSRASSEIIQEKYNIPTIIVDVIDVSSDSPAKTAGVK
                     RNWWIIGTIDENGKIRQVRGDLLLREIAKNKSDKITLEMFNPSDGQMKTVSFVPGCVH
                     TSSRMLAYVANIFPEPAKFSDRWPMLFWLLVIVVIITVLRDLLRFSQEYLVETTICKA
                     IMDLRCENYNAALHLPISYYTANGTSDTMSRFIADTNSLSKGLVTLFSKTMVEPAKAV
                     ASIIGAFLLMPKFMLIVCLAGPPVVIMIKLLGKRMKKASRRALENWAVMLAVLEETLV
                     GIRVVKTYTMEAAERKRFYRTNKTLFKQQRRMARIDAAIAPLVEAFGITAGMVAAGFA
                     GWAVLKS"
     gene            complement(1246..>2039)
                     /locus_tag="PLK08_09960"
     CDS             complement(1246..>2039)
                     /locus_tag="PLK08_09960"
                     /inference="COORDINATES: protein motif:HMM:NF020680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HPS53864.1"
                     /translation="SFLIDPAYQAASMGRGRIFVVEETDLMSIAAQNSLLKTLEEPPA
                     GVTIILLTDKPDQLLPTTRSRCAFLRFVPLPHDFVIARLVENEVSQEEAQFWAAFTDG
                     SVGLALEYAKQNFYPVKRELISDIARLAEGYVPLGDDWSKIADSLADAQVGVVKKEAN
                     ANLSKNLAMRQVTGIMLRLIAGAFADALTLATGSTPPLMHADQSQEIQRIAKNHRAAD
                     LAMIIERLSEYERLLWRNVNAKTVWDNAAISCSAGVGNITAEIFK"
BASE COUNT          484 a          539 c          500 g          516 t
ORIGIN      
        1 cacttttgag cacagcccag ccggcgaatc ccgcagccac catgccagcc gttatgccga
       61 acgcctccac aagcggagca atggctgcgt ctatgcgggc catgcgacgc tgctgcttga
      121 aaagagtttt gttggtgcga taaaaacgtt tgcgctcggc ggcttccatg gtataggttt
      181 tgacaacacg tatgccgacg agagtctcct ccagcacagc cagcatgact gcccagtttt
      241 caagtgcgcg acgcgacgcc tttttcatac gtttgcccaa taattttatc attatcacta
      301 ccggcgggcc ggcgaggcag acgataagca tgaatttcgg catgagcagg aaggcaccga
      361 ttattgacgc gacggctttg gcaggctcga ccatggtttt actgaacagc gtaacaaggc
      421 ctttggacag cgaattggtg tcggcaataa aacggctcat ggtgtcgctt gtgccattgg
      481 ctgtataata acttatcggc agatgaaggg cggcattgta attttcacaa cgcaaatcca
      541 tgatggcttt gcagatggtg gtttcgacca gatattcctg gctgaaacgc agcaggtcgc
      601 gtaaaaccgt tattattaca actatcacca acagccagaa aagcatcggc caacggtcgg
      661 agaatttcgc aggctcagga aatatgttgg cgacgtaagc cagcattcgt gacgaagtat
      721 gtacgcaacc cggcacaaat gaaacagtct tcatctgccc gtctgacggg ttgaacattt
      781 ccagcgttat tttgtcggat ttgttttttg cgatttcgcg caaaagcaag tcgccgcgaa
      841 cctgtctgat tttcccgttt tcgtcgattg tacctattat ccaccaatta cgtttgactc
      901 cggcagtttt ggccggcgaa tccgagctga cgtctatgac gtctacgatt atggtcggta
      961 tattatattt ttcctgtata atttccgaac ttgcgcggct ggtgagcttg gccatgagtt
     1021 tgtcttcact gatggaatcc catgcccagc cgtgcaggcc ttcctcgctc agaaggattt
     1081 ttaacgccgg cagagccatg ccgataccgc cgccccaaag acaggcaatc atgactacaa
     1141 gcaaaaatgc cgccacgata ttgagcttat agggaagaac atatttcatt gatcgcataa
     1201 aatatttcat atcgccatcc aatttatttc aggaattttg agtaattatt taaaaatttc
     1261 agcagtaata ttcccaactc cggccgagca ggaaatagct gcgttatccc aaaccgtttt
     1321 cgcgtttacg ttccgccaga gcagccgttc atattcgctc agccgttcga taatcatcgc
     1381 cagatctgcc gcacgatgat tttttgctat tctctgtatt tcctgcgact ggtcggcatg
     1441 catgagcggc ggtgtcgaac cggtagccag cgtaagcgca tcggcaaacg cacctgctat
     1501 cagccgcaac attatgcccg tcacctgccg catcgcaaga tttttggaaa gattggcgtt
     1561 agcctctttc ttcacgacgc ccacctgcgc atcagccagc gaatcggcga tttttgacca
     1621 gtcgtcgccc aaaggaacgt atccctcggc cagcctggcg atatcgctta tcaattcgcg
     1681 tttgacggga taaaagttct gttttgcgta ttccagcgca aggccgacgc tgccgtcagt
     1741 aaatgccgcc cagaattgcg cctcttcctg cgatacttca ttttcaacca gcctggcgat
     1801 tacgaagtca tgcggcagcg gcacgaatct caaaaaagca caacgactgc gggttgtcgg
     1861 cagaagctgg tcgggcttgt ctgtaagcaa aatgatagtc acgccagccg gcggttcttc
     1921 aagcgttttt aacagggagt tctgcgcggc gatactcatg aggtccgttt cctcgacgac
     1981 gaatatccgg cccctgccca tcgaagccgc ctggtaggca gggtctatca ggaaacttc
//
LOCUS       DAONFI010000377         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_74645_length_2032_cov_3.431968, whole genome shotgun sequence.
ACCESSION   DAONFI010000377 DAONFI010000000
VERSION     DAONFI010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74645_length_2032_cov_3.431968"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..620)
                     /locus_tag="PLK08_09965"
     CDS             complement(141..620)
                     /locus_tag="PLK08_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPS53865.1"
                     /translation="MSKVTNIIVAGLGGQGVLKCSDIIADAAFLNGCDVKKAEVHGMS
                     QRGGSVACDVRFGDEVLSPMVPLGQADYLLVLSDGQTENNIHNLKKGGVLITADLIDV
                     EKLENKRSLNVAILGLLSNYLTISEETWLKAIKRQLPEKIHAVNINAFELGRKSSRP"
     gene            complement(626..>2032)
                     /locus_tag="PLK08_09970"
     CDS             complement(626..>2032)
                     /locus_tag="PLK08_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPS53866.1"
                     /translation="AGAAFGGARVIVTMKHVGLNVAADPLFTLAYTGVSGALVIVSAD
                     DPGMASSQNEQDNRRYALAAGIPMLEPADSQEAYDFTAKAIELSEKFATAVILRMTTR
                     VCHSRTVVAPHANPSKPKQPQFVRDRQKQVMIPAYARLAHKRLRQRLADIQAWNETSD
                     LNRIYGDDSELGIIASGITAIHTREAASTAKILKIGMTYPLPIETVRKFAGSVNRCLV
                     IEEGDPFIFENIMAAGIAVEKAPKMYRFGELSVHRIQRILANDTSPEAAPPKGKAPEL
                     CPGCPHRKTFEMLRDMGCIVAGDIGCYTLGVLPPFEAIDTCVCMGASIGVGLGLRHVL
                     NEEQARKVVSVIGDSTFMHSGITGIVEMAYNPPKTGHVVLILDNSTTAMTGLQENPAT
                     GRKLDHTPANRVSIENLCRSLGVKNIFVFDPVTQGEELRNCVMDAMASNDLTVVIVRR
                     SCILAAKKYAELKKAAGE"
BASE COUNT          364 a          544 c          582 g          542 t
ORIGIN      
        1 agcgataccg tcgccatgag ccgcctgagc tgcaactccc gtaattcctc cgacggcaga
       61 taatccatcg ccgaggcagg attgaaattc tttttattgc tgtcgcaact gcacgtacat
      121 tctgaagtca tatcgtcacc tcagggccga ctgctttttc ggcccagttc gaacgcattg
      181 atgttaacag cgtgaatctt ttcgggcaac tgtcttttga ttgcctttag ccatgtttct
      241 tcacttatag tcagataatt gctcagcagg ccgagtatgg ccacgttgag cgaacgtttg
      301 ttttcaagtt tttcgacgtc gataaggtcg gcggttataa gcactccgcc ctttttgaga
      361 ttgtggatgt tgttttccgt ttggccgtcg ctgaggacaa gcaggtaatc cgcctggccg
      421 agcggcacca ttgggctgag gacttcgtcg ccgaatcgca cgtcgcaggc tacgctgccg
      481 ccgcgctggc tcatgccgtg aacttcagcc ttcttgacgt cacaaccgtt cagaaacgcc
      541 gcgtcagcga tgatgtcgga acattttaaa acgccctggc cgccgagtcc ggccactatt
      601 atgttggtta ccttgctcat ggttgtcatt ctcctgccgc ttttttgagt tcggcgtatt
      661 tttttgcggc cagtatgcac gaacggcgga cgatgacgac cgtcaggtca ttactggcca
      721 tcgcatccat gacacagtta cgcagctctt caccctgcgt gacggggtcg aatacgaata
      781 tgttttttac gccgagcgac cggcagagat tttcaatgct tacccgatta gccggcgtat
      841 ggtcgagctt tctgccggtg gctggattct cctgcaagcc ggtcatcgcc gtcgtgctgt
      901 tgtccagtat caaaaccaca tggccggtct ttggcgggtt gtacgccatc tcgactatgc
      961 cggtaatgcc gctgtgcatg aacgtgctgt cgcctatgac gctcactact ttgcgagcct
     1021 gttcctcgtt cagcacatgt cgtaacccga ggcctacgcc gatgcttgcg cccatgcaga
     1081 cgcaggtgtc gatggcttcg aaaggcggca gtactccgag cgtatagcac ccgatgtcgc
     1141 cggcgacgat gcagcccatg tcgcgcagca tttcgaacgt cttgcgatgc ggacagccgg
     1201 ggcatagttc cggtgcctta cccttgggcg gggcggcttc gggagaagtg tcgttcgcca
     1261 ggatgcgctg tatgcgatgt acgcttaatt cgccgaagcg gtacattttt ggggcttttt
     1321 ccacggcgat gccggcggcc atgatgtttt caaaaataaa cgggtcgcct tcttctatta
     1381 cgaggcagcg gttgacgctg ccggcgaatt tgcggactgt ttcaatcggc agcggataag
     1441 tcatgccgat tttgaggatt ttggcggtcg atgcggcttc ccgcgtatga atagccgtta
     1501 tgccgcttgc gattatgccc agttcactgt cgtcgccgta tattctgttg aggtctgacg
     1561 tttcgttcca cgcctgaata tccgccagcc gttgtcgcag gcgtttgtgt gccagccttg
     1621 catatgccgg tatcatcacc tgtttttgac ggtcgcggac gaattgcggc tgtttcggct
     1681 ttgatggatt tgcatgcggc gcgacgactg tacgcgaatg gcagacgcgg gtggtcatgc
     1741 ggagaattac ggcggtcgcg aatttttcgg agagttcgat tgcttttgcg gtaaaatcgt
     1801 aggcttcctg gctgtccgca ggttcgagca tgggtatgcc ggctgcgagt gcataacggc
     1861 ggttatcctg ttcgttctgg ctggacgcca tgcccgggtc atcggcggat actatcacga
     1921 gtgctccgct tacgccggta tatgcgagtg tgaacaacgg gtcggctgct acgttaaggc
     1981 cgacgtgttt catggtgacg atgactcttg ctccgccgaa ggctgcgccg gc
//
LOCUS       DAONFI010000378         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_76796_length_1996_cov_4.866048, whole genome shotgun sequence.
ACCESSION   DAONFI010000378 DAONFI010000000
VERSION     DAONFI010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76796_length_1996_cov_4.866048"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLK08_09975"
     CDS             complement(<1..1181)
                     /locus_tag="PLK08_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53867.1"
                     /translation="MRKYPLCDTAIYICIAAMISATLPARTLADGNPLDIVKKLISDK
                     SEKTSPSDKSESNAKIPSEPSRLGEFCVISGYDGSLRTGRCNGAVGGGLFIAPTSALG
                     DKITQGMVIYKGQIYPVNAVYKDSDYGVTVLFADADACGKSMTMGKFPEAGSKLLVNS
                     QTKFIGEVRDIYGIGAMRAIQLSGEVDEENFVGCEVYDARGRVVGIVTSCEVVYDKNK
                     RADICYILPSSCMNQMANDAFKNKVSLQKPAENILSKITSAQFQSYSQARQMLYLRRY
                     PQAIKLLRKLLNSKPVSAYAYRQLGDMYFEQHDFPRAAECYKNAIDIQPSLLACYPQM
                     AQAMGKTGQPAKALDILLKCSKSIMGDEMKQTFAESFRLVGLDLIRSKLPQKAVVALQ
                     NA"
     gene            complement(1525..>1996)
                     /locus_tag="PLK08_09980"
     CDS             complement(1525..>1996)
                     /locus_tag="PLK08_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HPS53868.1"
                     /translation="RVCLAGAAVDDGVVTCSHAGGGSGVKARVVAKYLGGCGAGRCYI
                     AAEPNGNITPCVYLPHRVMGNVREKPLERIFRESVFWEILNDRKRRLGHCEVCAFSDY
                     CGGCRARADAYFGKLHAADPGCIFNQKHWDKLVEDGIAIVETESADAKTEISSQ"
BASE COUNT          429 a          509 c          505 g          553 t
ORIGIN      
        1 gcattctgca gggcgacgac agccttttgc ggcagtttcg acctgatgag gtcaaggccc
       61 acaagcctga acgattcggc gaacgtctgc ttcatttcgt cgcccatgat gcttttggag
      121 cacttcagga gtatgtcgag cgctttagcc ggctggccgg ttttgcccat cgcctgcgcc
      181 atttgcgggt aacacgccag caatgacggc tgaatatcta tggcgttttt gtagcactct
      241 gcggctctgg ggaagtcgtg ttgctcgaaa tacatgtcgc ccaactgccg gtaggcgtag
      301 gcgctgacgg gtttggaatt cagaagttta cgcagcagtt ttatcgcttg cggataacgc
      361 cgaaggtaca gcatctgcct ggcttgcgaa tacgattgaa actgtgcaga cgtgattttt
      421 gacagtatgt tttcagccgg cttttggagt gaaaccttat ttttaaaggc gtcattcgcc
      481 atttgattca tgcacgacga aggcagaatg tagcatatgt cggctcgctt gttcttgtcg
      541 taaaccactt cgcaactggt tacgattccg actactctgc ctctggcgtc gtagacctcg
      601 cagccgacga aattttcctc gtcgacctca ccggagagct gtatggcccg cattgcgccg
      661 atgccgtaaa tatctcgcac ttccccgata aatttcgtct gtgagttaac cagcagttta
      721 gagcctgctt cgggaaattt ccccatagtc atcgattttc cgcaggcatc ggcgtcggcg
      781 aaaagcaccg ttacgccgta atcggaatct ttgtaaacgg cgtttaccgg atatatttgg
      841 cccttatata tgaccatgcc ttgcgttatt ttgtcgccca aagcgctggt tggcgcaata
      901 aacaaaccgc cgccgacagc tccgttgcat cggccggtcc gcaatgagcc gtcataaccg
      961 cttatgacgc agaattcacc caatctcgaa ggttcggagg gtattttggc gtttgattcg
     1021 gatttgtcgg atggcgatgt tttttctgat ttgtctgaaa tcagtttttt gacgatatcc
     1081 agcggattgc cgtcggcgag agtccgtgca ggtaatgtcg ccgatatcat cgctgcgata
     1141 cagatatata ttgccgtgtc gcacaaagga tacttccgca ttcttcacct cgtttcaaat
     1201 acgctcctgc gggttatttc agagcacttc aaattcctgc gcatcagttt gaacaattat
     1261 ctcaaagaga ccaattcgcg ttataaggtt cggtaaaaac ttattttgca agcatttacg
     1321 atatgcatga caaataattc tgcgacgtcg cctttgtgag ccgcggtaat gtattttatt
     1381 gcaaaataag tcttaccttt tagcctcata tgcgaaaaat cgtaaatcgg tcagtttgaa
     1441 caattatata tcgtcatgat gacggcagat actgaattat ataccggata ttctttcgat
     1501 accaacttct ctgtttgtcg caggttattg tgaagatatt tctgtttttg catcggcgga
     1561 ttcagtttcc actatcgcta tgccgtcttc cacgagttta tcccagtgtt tctggttaaa
     1621 tatgcagcct gggtcggcgg cgtgcagctt gccgaaatat gcgtccgcgc gtgcgcggca
     1681 gccgccgcaa taatctgaaa atgcgcagac ttcgcaatgg cccagccgcc gtttgcggtc
     1741 attgagtatc tcccagaaga cgctttcgcg gaaaatacgt tcgagcggtt tttcccgcac
     1801 attgcccatg acgcggtggg gcagataaac gcacggcgtt atgttgccgt tgggctcggc
     1861 ggcgatgtag catcgtcctg cgccgcagcc gccgagatat ttggctacca cgcgtgcctt
     1921 gactccgctg ccgccgcctg cgtggctgca cgtcacgacg ccgtcatcga cggctgcgcc
     1981 ggctagacat acgcgg
//
LOCUS       DAONFI010000379         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_78035_length_1976_cov_4.260281, whole genome shotgun sequence.
ACCESSION   DAONFI010000379 DAONFI010000000
VERSION     DAONFI010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78035_length_1976_cov_4.260281"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..996)
                     /gene="argF"
                     /locus_tag="PLK08_09985"
     CDS             complement(64..996)
                     /gene="argF"
                     /locus_tag="PLK08_09985"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPS53869.1"
                     /translation="MKTDFINLTDFSSTQLQKLIDRTIEEKPLYRAGCGPAPLERKTL
                     AIIFEKPSLRTRVSFEVAMTQLGGTTTYLDQMSIGIGVRESVRDIARVLSRMCDGIMA
                     RTFSHKLVEELAKFSSVPVINGLTDYSHPCQAMADLVTVQETFGSLKGRTLVFVGDGN
                     NVARSLAIGCSKLGMNFVLSAPEGYTLEEDFFPQLKKENSLFDCQLIANPLEAVRNAD
                     VIYTDTWVSMGQEDEKAERLKIFRDYQVNMKLLAAAPEHVIVLHCLPAYKGFEITEDA
                     FEKHAEVIMEQAENRLHFQRTLLAALMGKDKKSD"
     gene            complement(1032..1904)
                     /gene="argB"
                     /locus_tag="PLK08_09990"
     CDS             complement(1032..1904)
                     /gene="argB"
                     /locus_tag="PLK08_09990"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPS53870.1"
                     /translation="MQKAIEKAHALIEALEYIQKYAGKVIVIKFGGSVMDDPVAMKNI
                     LTDVVFMSSSGMMPVIVHGGGKAISKAMEERNLEVQFVQGRRYTDQRTLTVVEHVLCN
                     EINADIVKTLNSLGAKALPLHTLSSCVLFGEKLFLEEDGRKIDLGQVGKITRVGAEVI
                     RSITSDHIIPAIAPLAIDHKGGKLNCNADTAAGEIAAELRAEKFVVISDTHGIRKNVK
                     DPNSMIPEITQDEIRNLIADGTITSGMLPKVEACCRALDGGVKRTHIIDGNIEHALLL
                     EIFSRKGVGTLIQK"
BASE COUNT          381 a          485 c          559 g          551 t
ORIGIN      
        1 gcaggcagcc aaggtttttg ggataggttc tatttacgaa tggacgatta atggccggcg
       61 gaatcaatca gattttttat ccttgcccat caatgccgcc agcagcgtac gctggaagtg
      121 caggcggttt tcagcctgct ccataatgac ttcggcgtgt ttttcgaatg cgtcctcagt
      181 tatttcaaag cctttgtatg ctggcagaca gtgaaggact atgacgtgtt ccggcgctgc
      241 tgcgagaagt ttcatgttga cctggtaatc gcggaagatt tttaaacgtt cggccttttc
      301 gtcttcctgg cccatggaga cccatgtgtc ggtgtagatt acgtcggcgt tacggacggc
      361 ttcaagcggg ttggcgataa gctggcagtc gaagagggag ttttccttct tgagctgcgg
      421 gaagaaatct tcctccagcg tataaccttc gggtgcggac agcacgaaat tcatgcccag
      481 cttgctgcag ccgatagcca ggctgcgggc tacgttgttg ccgtcgccca cgaatacgag
      541 cgtcctgcct ttgaggctgc cgaaggtttc ctgcacggtt acgaggtcag ccatcgcctg
      601 gcaggggtgc gaataatcgg tcaagccgtt gatgaccggc acgcttgaaa atttggccaa
      661 ctcttccacg agtttatggc tgaacgtgcg agccataatg ccgtcgcaca tgcgcgacag
      721 aacgcgcgct atgtcgcgga cgctttcgcg aacgccgatg ccgattgaca tctggtccag
      781 atacgtcgtc gtgccgccga gttgagtcat ggcgacttca aagctcacgc gagtacggag
      841 ggaaggcttt tcgaaaatta tggcgagggt tttgcgttcc agcggggcag ggccgcagcc
      901 ggctcgatag agcggctttt cctctatcgt ccggtctatt agcttctgaa gctgggtgga
      961 tgaaaaatcc gtcaaattta tgaagtctgt tttcatggtt gtattccgtt tcaataattg
     1021 tcggttacgg attacttttg tatcagtgtt cctacgcctt tgcggctgaa tatttccagc
     1081 agcagggcgt gttctatatt gccgtcgatg atgtgggtgc gttttacgcc gccgtcgagt
     1141 gcgcggcagc acgcttcgac tttcggcagc atgcccgacg ttatagttcc gtcggcgatg
     1201 agattgcgga tttcatcctg tgtgatttcc ggtatcatgc tgtttgggtc ttttacgttt
     1261 ttgcgtatgc cgtgcgtgtc ggatatgacg acgaattttt cagccctgag ttcggcggct
     1321 atttcgccgg cggcggtgtc ggcgttgcag ttgagtttgc cgcccttgtg gtctatggct
     1381 aacggcgcta ttgcgggaat tatgtgatcg ctggttatgg agcggatgac ttccgcgccg
     1441 acccgcgtaa ttttgccgac ctggccgagg tcgattttgc ggccgtcttc ttcgagaaaa
     1501 agtttttcgc cgaacagcac gcaactggag agggtgtgca gcggcaatgc cttggcgcca
     1561 agcgagttga gcgtctttac tatgtcggcg ttgatttcgt tgcaaagcac gtgttctacg
     1621 acggtaagcg tgcgctggtc ggtgtagcga cggccctgca cgaactggac ttccaggtta
     1681 cgttcttcca tcgccttgct gatggctttg ccgccgccgt gaacgattac cggcatcatg
     1741 cccgatgaac tcatgaaaac aacgtctgta agaatatttt tcatcgccac cgggtcgtcc
     1801 atgaccgaac cgccgaattt gatgactatt accttgccgg cgtatttctg gatatattcc
     1861 agcgcctcaa tcagggcatg tgctttttct attgcttttt gcatcttttg tccttttgcc
     1921 acaataagaa ccgtaaaagc taacatttgg gcccttttgt gtcaaatgaa tatcag
//
LOCUS       DAONFI010000380         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_78677_length_1965_cov_4.618848, whole genome shotgun sequence.
ACCESSION   DAONFI010000380 DAONFI010000000
VERSION     DAONFI010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78677_length_1965_cov_4.618848"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1057..1848
                     /locus_tag="PLK08_09995"
     CDS             1057..1848
                     /locus_tag="PLK08_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53871.1"
                     /translation="MKRKAFTLIELLVVIAIIALLVSILMPSLNTARELAKRAMCCTN
                     LNGIGKALMLYSSSNDDKYPGYLKKSTDTTTGSPDGSYPASYITYSETGSNTLNANSY
                     ALLAKDGFVSYAIFKCPSVSDTETAQPSTSDDEFGFTGSKQVSYGFQPASGSSATAQS
                     ADISLSEDLGGTVIIAGDRYGSAASITTTSGQTNVSSTQNHVSGKKGQENMLTSGGGV
                     KSEKFNDYSSTTCKSNWGSCDDDYIFKLDNSTEVGKYDSLLIYIK"
BASE COUNT          546 a          408 c          441 g          570 t
ORIGIN      
        1 gcaatatatt gtatacttca agtaaaaata tcccaaatat tgcgtatttt ttataatacg
       61 ttgtgaatta tgcatttacg aacaatactt tttgcgagtt tttgaattat caacaatttt
      121 ttacatccct tcgcataagg ctatacaata aatggtatgc tcttcaggga actctactat
      181 ctattgtacc acaacttcgc gacaatatag ccgtaattcc tgaatgaaat cacaaaagct
      241 cccttgattg gataacatga atcggtcatt ttgagtaatg tcctataaaa aatgcttcaa
      301 tatgggttat tgataaattt tacaacttat gttataattt ctgtgaataa agtggatggt
      361 tgccggaggc gttttttgtg gggcaaaccg ctataagtcg aataataaat taaatcgaac
      421 ttctggataa ccacttctgc tttactctta ttgttgtaca tgcaaacggt ttttatggct
      481 gtcgaaacta tattagccgc agttgacgtc agcctgccgg gtggcaacta tatgaacggt
      541 tgccaaaaaa gtgctttttt ggcgaaaaac cgcttgcttg gcttgaaaat atttgataat
      601 ctgcttcttt tgtattattt cagctcgtgt tttaacgccg gctgctaata aggaaattcc
      661 gtgataggcg catttcggaa tctgaacatt gacaaataaa tgacggctgg ttgcggttgt
      721 tcgggcggag tcgtccggct gcggccagtt ggagatggac gcgaagttgt cgtcttcttc
      781 aaaaaactcg cgtacggcat cggcatatca gaatcgtttt gctgcaacag ctataataat
      841 tttaaaaaaa cacgccgagc tggagggaga gtgtttttga atgagcagac gtcatcgtcg
      901 gggaagtctc cgcacaacgc aacaactgcc ttgaaaaatt ggttcgatgc atgcgccgca
      961 gaagatggaa cctgccatat tagataattt atgctgggcg tttgtggcgt ccaaacctca
     1021 aggtaatacg ttgcgaatta tgtaaggagt tttacgatga aacgtaaagc gttcacgctg
     1081 attgaactgc tcgtcgttat cgccatcatc gcgttgttgg tgtcgatatt gatgcccagc
     1141 ctcaatacgg caagagagtt ggccaagcga gccatgtgct gcaccaacct caacggcatt
     1201 ggcaaagccc tgatgctgta ctctagttca aatgacgaca aatatccggg ctatctcaaa
     1261 aagtcgactg atacgactac tggcagtccg gatggttcgt atccggcaag ctacataacc
     1321 tacagcgaga ctggcagcaa tactcttaat gccaatagtt atgcgttgct cgccaaggat
     1381 ggatttgtct cttatgccat cttcaaatgt ccttcggtca gcgatactga aacggcacag
     1441 ccgagtacga gcgatgacga gttcggattc accggttcaa agcaggtttc atatggattt
     1501 cagcctgcta gcggttcttc ggcgacggct caatcagccg atatctcgct gtctgaagat
     1561 cttggcggaa cggttattat tgccggcgac agatacggtt ctgcagccag tatcacgact
     1621 acttcaggcc agaccaatgt atcttcgacc cagaaccatg tttcaggcaa gaaaggccag
     1681 gaaaacatgc tgacatccgg cggcggagtc aaaagcgaaa aattcaatga ctacagctcc
     1741 acgacgtgca aaagcaactg gggctcgtgt gacgatgatt atattttcaa acttgacaat
     1801 tccacggaag ttgggaagta tgattcactt ctgatttata tcaagtgatg attttatcac
     1861 tttttctttc aggcaaaacg attcttaaaa ggcggtcaga tttggccgcc ttttttaata
     1921 tgtccttcgt agttctacgg tttttaaacc gtagatgaaa gtatc
//
LOCUS       DAONFI010000381         1952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_79561_length_1952_cov_4.007380, whole genome shotgun sequence.
ACCESSION   DAONFI010000381 DAONFI010000000
VERSION     DAONFI010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79561_length_1952_cov_4.007380"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLK08_10000"
     CDS             <1..652
                     /locus_tag="PLK08_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53872.1"
                     /translation="IQPGDVTVSPAGVETSYSLAKRGRHLCIHFMPQRRTHNIISLPL
                     HIRLGYRTQQAAELFSQVSVAFMQYKMTGSDYAKLSASSCLQRLLAYLAEFVNSGDDR
                     AVRGLDYSVAERLAGIIDSEYVEPLSVPALARRAGMSQNYLARLFRVRYGVTIPRYIL
                     TVRIEKAKELLEITNLKVGCVGQYVGLRDPHYFNKMFRKLAGMNPSEYRKISRVDA"
     gene            801..1403
                     /locus_tag="PLK08_10005"
     CDS             801..1403
                     /locus_tag="PLK08_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPS53873.1"
                     /translation="MIVHEKYEWSNNWWNNADDKLLKRVLIIGDSISCCYHPVVIKKL
                     RGKVNVDRVANSRSLDDPMLFGEVSLALENCRYQVVHFNNGLHGWHLTDAGYRRLLAR
                     YVELIREKAPRSRLIWATSTPVTKINKPHLPCMEKNPRVIRRNEIAVKIMAKYDIEVN
                     DLYSLVFGNAGIRAKDGYHYNAGGAELMGRAVAGAIRRYL"
     gene            1479..>1952
                     /locus_tag="PLK08_10010"
     CDS             1479..>1952
                     /locus_tag="PLK08_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53874.1"
                     /translation="MKKIVKKSWPLLCALLIFWTAVAVLMFMSVRKTGGHLIYPLDDT
                     YIHMAIAKNVALHHVWGVTGYGFSSSTSSPLWTGLLAAGYSVFGVNDVLPFILNLLFG
                     TFAVIAAYELILKYTGGRVKIFIILTSAILAAPLPALTISGMEHVLHAFLPICFIY"
BASE COUNT          471 a          427 c          519 g          535 t
ORIGIN      
        1 aattcagccc ggcgatgtaa ccgtttctcc ggctggcgtt gaaacgagtt attctctcgc
       61 caaacgcggc cggcatttat gcattcattt catgccgcaa cgacgtacac ataacattat
      121 atcgctgccg ctgcatatac gcctgggtta caggacacaa caggcggctg aacttttcag
      181 ccaggtaagc gttgctttca tgcaatataa aatgacgggg tcggattatg cgaaactttc
      241 tgcgtcgagc tgcttgcagc ggctgcttgc gtatttggcg gaatttgtaa atagcggcga
      301 cgatagggct gttcgcgggc tggattattc cgtcgccgaa aggcttgccg gcatcataga
      361 ttcggaatac gtcgagccgt taagcgtacc ggctttggct cgtcgcgctg gcatgtcgca
      421 aaactatctg gcgaggctgt ttcgcgtacg ttacggcgtg acgataccgc gatacatact
      481 tacagtgcgg attgagaagg ctaaggagct tctggagatt acaaatctga aggtaggttg
      541 cgtcggtcag tatgtcggct tgcgagaccc gcattatttc aacaagatgt tcagaaaact
      601 ggctggcatg aatccgtcgg aatatcgtaa aatcagcaga gttgatgcct gaaaagtgga
      661 attttttgga caaaaccaaa ggctgatgct ttaatatgtt ttcgcggctg gcgcgattct
      721 caaaagaaaa ttttttaaga accgttcagc ctaaatgttt tcgtataagt attttgttat
      781 tttttaatgc aggtggaaaa atgattgtac atgaaaaata tgaatggtct aacaactggt
      841 ggaacaatgc cgatgataaa ttgttgaagc gggtgctgat tatcggcgat tcgataagtt
      901 gctgttatca tccggttgtg ataaagaagc tcaggggaaa ggtcaacgtc gacagagtgg
      961 ctaactcgcg cagcctcgat gatccgatgc ttttcggcga ggtcagcctt gcgttggaaa
     1021 attgcagata tcaggttgtg cattttaaca atggtttgca cggctggcat ctgacggatg
     1081 ccggctatcg caggctgttg gccagatatg ttgaacttat cagggaaaaa gccccccgtt
     1141 ccaggcttat atgggctact tccacgcctg ttacgaaaat caacaaaccg catctgcctt
     1201 gcatggaaaa gaatccgcga gtcataaggc gcaacgagat tgccgttaaa atcatggcga
     1261 agtacgacat tgaagtgaat gacctgtatt cgctggtttt cggcaacgcc ggtattcgcg
     1321 ccaaagacgg ttaccattat aacgccggtg gcgcagaact tatgggccgc gcggtggctg
     1381 gcgccataag acgatacctt taatcccatt tttgaaacag cttccgtatg taatagtgcc
     1441 atgctgatta gccggtagcc ttgataacct ggagcgcgat gaagaaaatc gttaaaaaga
     1501 gttggccgtt attgtgtgcg ttgctgatat tctggacggc tgtggcggta ttgatgttca
     1561 tgtccgtcag aaaaaccggc ggtcatttga tttatcctct ggacgacaca tacattcaca
     1621 tggccatagc caaaaacgta gcgcttcatc acgtatgggg cgtgaccgga tacggctttt
     1681 catcctccac ttcttcgccg ttgtggacgg gtctgcttgc cgccggctat tccgtgttcg
     1741 gggtgaatga cgttctgccg ttcatattga acctgctgtt cggaacgttt gccgtaattg
     1801 ccgcgtatga gttgattttg aaatataccg gcgggcgtgt gaaaattttc attattctga
     1861 cgtcggcgat tctggctgcg cctctgccgg cgttgacgat aagcgggatg gagcacgttc
     1921 ttcatgcatt tcttccgatt tgcttcatat at
//
LOCUS       DAONFI010000382         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_80016_length_1945_cov_3.296296, whole genome shotgun sequence.
ACCESSION   DAONFI010000382 DAONFI010000000
VERSION     DAONFI010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80016_length_1945_cov_3.296296"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PLK08_10015"
     CDS             complement(<1..1090)
                     /locus_tag="PLK08_10015"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPS53875.1"
                     /translation="MSRIDKKGYKSGSIVWADRQTAEELRSCLSMFSSPASAGWNRIK
                     QNPSRTVWQGRLPNGGEVFLKQFHSRSTLHNLKSRLTKSDAKREFEFSRILAANGIAT
                     ARVLAYRNDNLSQWILTESLNPAMPLDKWHEQQLNGGSAGKQRIRVCINMLAEFLAKM
                     HAAGIEHRDLHCGNIFLTKNGNDVRLAVMDLHRIRLHGRMHRRRRSINMAMLMHDRLA
                     STTRTERLKFLRTYMKAAKVKGTLRGWQWLVEFFWAKHAGRLYRQRDRRVLGRKNLYF
                     ARLKLKNHWKGHVILASKRRMLNSRAAKMEFRITDWQQLLRQPEKLLYGDDVEVVKES
                     ASVRVIRRIITIGDKQVGMPRGGCTTGLF"
     gene            complement(1098..>1945)
                     /locus_tag="PLK08_10020"
     CDS             complement(1098..>1945)
                     /locus_tag="PLK08_10020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPS53876.1"
                     /translation="NAIFHVWSYSLLELAAHAAELCGAKVLLSLPALPLEKKPQKYLP
                     WDLGRFRAGLTLPTHTARQTLVEMHADPKHIFVLPPAAEIISDRLERRTRMRASLGIS
                     SATAVLATAGPLYRMGPQKMAAWALGILRNVMSDLKLIYPGKGPDEKNIRRFAATTGY
                     IHDIFYTGERFDYIDAMCAADVVLCCNQQDVGINGAAAALAASCATIVSDTPDLQDFC
                     ADKKDAVLTCRAGDPAAVASEILKLLDDEQLRDTLSQRAANTMHGNSPDAVRGTLENI
                     YAGLK"
BASE COUNT          360 a          537 c          545 g          503 t
ORIGIN      
        1 tgaacaagcc ggtggtgcag ccgccgcgcg gcatgccgac ctgcttgtcg ccgatggtta
       61 ttattcgccg tattacccgt accgaggcgg attccttgac cacttccacg tcatcgccgt
      121 agagcagttt ttccggctgc ctgagcagtt gctgccagtc ggtaattcgg aattccatct
      181 ttgcagccct gctgtttagc atgcgtcgtt tcgaggcgag tattacgtgg cctttccagt
      241 ggtttttgag tttcaggcgt gcaaaataga gatttttcct gcccagcaca cgtcggtcgc
      301 gctgacggta tagccggccg gcgtgtttgg cccagaaaaa ttccaccagc cactgccagc
      361 ctctcaatgt gccctttacc ttcgccgctt tcatgtatgt acgcagaaat ttcaatctct
      421 ccgttcgcgt cgttgacgcc aggcggtcat gcatgagcat cgccatgttt attgaccggc
      481 ggcggcgatg cattctgccg tgcagacgta tgcggtgcag atccatcacc gccaatcgca
      541 cgtcattgcc gtttttagtc agaaagatat tgccgcagtg caggtcgcga tgttcaatgc
      601 cggcggcgtg cattttagcc aaaaattcgg cgagcatgtt tatgcatacc cgtattcgtt
      661 gtttgccggc tgagccgccg tttagctgct gttcgtgcca cttgtccagc ggcatggccg
      721 gattgagcga ttcggtgagt atccattggc tgagattgtc gttgcgatac gccagtaccc
      781 tggctgtggc tatgccgtta gccgccagta tgcgtgaaaa ttcaaactca cgttttgcgt
      841 cgctttttgt caggcggctt ttgagattgt gcagagtgct gcggctgtgg aactgtttga
      901 ggaacacttc cccgccgttc ggcaggcggc cctgccagac ggtgcgagac ggattctgtt
      961 ttatcctgtt ccagccggct gacgccggcg agctgaacat cgacagacaa cttcggagtt
     1021 cttctgcggt ctgcctgtca gcccatacga tactgccgga tttgtagcct tttttgtcta
     1081 ttctgctcaa aatttcctca ctttaatccg gcgtatatat tttccagagt gccgcgaacg
     1141 gcatccggag agtttccgtg cattgtgttt gcagcccgtt gcgagagagt atcgcgaagc
     1201 tgttcgtcat ccagcagttt cagtatttcg gatgcaactg ccgccgggtc gcctgcgcgg
     1261 cacgtcagga cagcgtcttt cttgtcggcg cagaaatcct gcaaatccgg cgtgtcggac
     1321 actatggtgg cgcaggacgc cgccagagct gccgctgctc cgtttatgcc tacgtcctgc
     1381 tgattgcagc acaatactac atcggctgca cacatggcgt ctatatagtc aaaacgttca
     1441 ccggtgtaaa aaatatcgtg aatgtaaccg gtcgtggcgg cgaagcggcg aatgtttttt
     1501 tcgtccggcc ccttgcctgg atatattagt ttgaggtcgc tcatgacatt ccgcaagatg
     1561 cccagcgccc aggcagccat cttttgcggc cccattctgt acagcgggcc ggctgtcgcc
     1621 aacacggctg tggctgacga tatgcccagc gatgcacgca tgcgggtccg gcgttcaagt
     1681 ctgtcgctga taatttcggc ggctggcggc agcacgaaga tatgtttcgg gtcggcgtgc
     1741 atttctacca aagtctgtct ggcggtgtgc gtcggcaggg tcagcccggc gcgaaagcgg
     1801 cccaaatccc acggcagata tttttggggc tttttctcaa gcggcaaagc cggcagggac
     1861 agcaggactt tcgctccgca taattccgcc gcatgtgcag ccagctcaag aaggctgtac
     1921 gaccagacgt gaaatatggc gtttt
//
LOCUS       DAONFI010000383         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_80075_length_1944_cov_3.288512, whole genome shotgun sequence.
ACCESSION   DAONFI010000383 DAONFI010000000
VERSION     DAONFI010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80075_length_1944_cov_3.288512"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLK08_10025"
     CDS             <1..732
                     /locus_tag="PLK08_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPS53877.1"
                     /translation="KNVITSLMADNNDIVTPWQEQLAELSVVVGGLAHEIKNPLSTIT
                     LNLKLLGEDLERFDDDEHRRLLRRLLCVQEETARIQRILDDFLQYAGKVELHAADTDI
                     CEVVREMRDFFTPQAEASRIVFRTQLPAQPVICKIDVNLIKQALLNLMINAVQAMNTG
                     GELIVRVTTKGAFANIEVIDSGPGIPPENLNLIFKAYFSTKKSGSGLGLPTARRILKE
                     HGGDMIVESEQGKGTCFLLKLPIVH"
     gene            866..1219
                     /locus_tag="PLK08_10030"
     CDS             866..1219
                     /locus_tag="PLK08_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53878.1"
                     /translation="MMKPYFCPKCGKAYSQKDCLINDGKMYCYDCPKTKLAFTGGPLM
                     LIGAFLGVLFISLQLNHTVQAIQYSKWAVLLAACGFVFTCILRNQQAYNARKHAQNNS
                     TQAEPEEKQGPISAE"
     gene            1377..>1944
                     /locus_tag="PLK08_10035"
     CDS             1377..>1944
                     /locus_tag="PLK08_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPS53879.1"
                     /translation="MKQLGIILASLTIVIAAGCDNAKKDFDDKLVEMGWKKKPEETIV
                     KERKAVIPSDIKGTIAEVASLVTGGDAPVGGIGVVVGLVKEGSSEVPPQFRSQLIKYL
                     KEEIHIGSAISDTSSVTPSMFLDDLDTAVVRVDAVIPPGVPRGTKIDVLISALPRTQT
                     QSLQGGVLLPLNLTWDPTGDISTRDLKTL"
BASE COUNT          557 a          464 c          465 g          458 t
ORIGIN      
        1 aaaaatgtta taacttcact catggccgat aataatgata tcgtcactcc atggcaggaa
       61 cagcttgccg aactatcagt cgtagtgggc ggtctggctc acgagataaa aaatccactt
      121 tccacaatta cactcaatct caaattactg ggtgaggatt tggaaagatt tgacgacgat
      181 gaacatcgca ggcttttgcg acggctgctc tgcgtacagg aggaaacggc ccgaattcag
      241 cggattctgg acgattttct gcaatacgca ggcaaggttg aactgcacgc cgccgatacc
      301 gatatatgcg aagtcgtacg ggaaatgcgc gattttttca ctccgcaagc cgaagcctca
      361 agaatagtct tcagaacaca gttgccagcc cagccggtaa tctgtaaaat tgatgtaaac
      421 cttataaaac aggcactgtt gaacctgatg ataaatgccg tgcaggctat gaacacggga
      481 ggcgagctca ttgtcagagt aacgacaaaa ggagcgtttg ccaacattga agtcatagat
      541 tccggcccgg gaataccgcc ggaaaatctg aatctgatat tcaaagccta tttttcaaca
      601 aaaaaaagcg gctcaggcct cggccttcca accgccagaa gaattttgaa agaacacggc
      661 ggcgacatga tagttgaatc ggaacagggc aaagggacgt gttttttgct taaacttccc
      721 atcgtgcact aaccgataat aagagaggta tttatttgct acacataatg ttttatctgt
      781 tcgcaagacc gagcgttatg taaatgttga aaaattcagg aacgtatcct gatatacatc
      841 acatttgtag gaacggagaa tgaaaatgat gaaaccatat ttctgcccca aatgcggcaa
      901 agcctacagt cagaaagatt gtcttataaa cgacgggaaa atgtactgct acgactgccc
      961 gaagacaaag ctggcattta cgggcgggcc tttgatgtta ataggcgcat ttctgggcgt
     1021 tcttttcatc tcgcttcaac tgaatcacac cgtccaggcg atacaataca gcaaatgggc
     1081 agttctgctt gccgcatgcg gttttgtttt cacctgcatt cttcgcaatc aacaggcgta
     1141 taatgcaaga aaacacgcac agaataattc cacgcaggca gaacccgaag aaaaacaggg
     1201 cccgataagc gcagaataaa actgacggct ctaaaaattc ctgtttgaat tcacaattcc
     1261 ccgctttcgc aaaaaggcgt ggagcttttc ttttttgttt ttatcacgct gtatgattgg
     1321 cttttcgcag tgattttgct atcattatcg caacctctgg catggagatt gtaacgatga
     1381 agcagctggg catcatttta gcgtcgctga cgatagtcat tgccgccgga tgtgataacg
     1441 caaaaaaaga tttcgacgac aaactggtag aaatgggctg gaagaaaaag cccgaagaaa
     1501 ccatcgtcaa agagcggaaa gccgtcattc cgtccgatat caagggcact atcgcagagg
     1561 ttgcgtcgct ggtaacaggc ggcgatgctc ctgtcggcgg cataggcgtc gtggtcggcc
     1621 ttgtcaagga aggctcgagt gaagtgccgc cgcaattccg ttcgcaactc ataaaatatc
     1681 tcaaggaaga aattcatatc ggcagtgcca tttccgatac aagttccgtg acgccgtcga
     1741 tgtttcttga cgatctcgac accgccgtcg tcagggtgga cgcggtgatt cctcccggcg
     1801 taccgcgagg cacaaaaatc gacgttctca tcagtgctct gccccgcact cagacccaaa
     1861 gtctgcaagg cggagttctg ctgccgctca atctaacatg ggaccccacc ggcgacatct
     1921 caacgcgcga cctaaaaacg ctgg
//
LOCUS       DAONFI010000384         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_80245_length_1941_cov_4.341994, whole genome shotgun sequence.
ACCESSION   DAONFI010000384 DAONFI010000000
VERSION     DAONFI010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80245_length_1941_cov_4.341994"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..994
                     /gene="holA"
                     /locus_tag="PLK08_10040"
     CDS             44..994
                     /gene="holA"
                     /locus_tag="PLK08_10040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPS53880.1"
                     /translation="MTANQTKPVYAIVGEDAFLKSQARQNIISAAIGDADPQMCVVQY
                     DPEVELATVLDELRTMPFLAPHRVVVVCEAEAFVAANRDGLQKYLEKPSSGGTLVLMM
                     NTLDKRLTVTKQIAAAGEVVDCNSPAGAELCKWVQDAVTAEGKKMSVAAAAMLCDMVP
                     ESVGELRNEIDKLVTYAWNRPEITEKDIAAIVTARSAPEAFAVVNAIIAADVASALKA
                     TGSAMAVRGAEFGLLGQLRWNIQRSLQAQQRISGGENQQAVFRSLRIFKDREFAAYLR
                     RRGLQGLQGDMKKLLQADLSMKSGTEPKTAMQQLIVALCN"
     gene            1045..1656
                     /locus_tag="PLK08_10045"
     CDS             1045..1656
                     /locus_tag="PLK08_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPS53881.1"
                     /translation="MSHIKAVLSDIGNVIMPVQTEFFIKNLVEKSGADMTVVNDVVRK
                     TIAVEHEINRGRRTIRQAFDEFIAQPLNIGYEEFLDSWYALLGEEYPQVREAYERLNV
                     PLYVLSNINDVHADYLHGRWFEEISIACWMSNEIGMDKPSREVFEFVLGQIKCQPSEL
                     LFFDDIAENVQSAASLGINAVLVKNPDDVVKKLEECKLLVGKS"
     gene            complement(1669..>1941)
                     /locus_tag="PLK08_10050"
     CDS             complement(1669..>1941)
                     /locus_tag="PLK08_10050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPS53882.1"
                     /translation="GVTSALLDGAMTNCLFAHGIVAVTAEMNVRFRHPIKLGMPLTLR
                     AKITHQQSPLYIVEAGLIQENQLRAKSVGKFMEQRDESERQLSMFS"
BASE COUNT          496 a          509 c          520 g          416 t
ORIGIN      
        1 agccttgtaa tccggcggcg ggttttgtat ccttgaaaca ccaatgacag caaatcagac
       61 aaaacccgtt tatgccatcg tcggcgaaga tgcatttctg aaaagccagg ctcgacagaa
      121 catcatatca gccgccatcg gcgatgccga cccgcagatg tgcgtcgtcc aatatgaccc
      181 tgaagttgaa ctggcgactg tgctggatga gttgcgcacc atgccgtttc ttgctccgca
      241 tcgcgtggtg gtagtgtgcg aggcggaagc gttcgtcgcg gcaaatcgcg acggcttgca
      301 gaaatatctc gaaaaacctt cgtccggcgg cactctcgta ctgatgatga acacgctgga
      361 caagcggctg acggtgacaa aacaaatcgc cgcagccgga gaagtcgtgg actgcaactc
      421 acctgccggc gcggagcttt gcaagtgggt tcaggacgcg gtaacggctg agggcaaaaa
      481 aatgtcggtg gctgctgctg cgatgctgtg cgacatggtg ccggaaagcg tcggagaact
      541 gcggaacgaa atcgacaagc tcgtcaccta tgcatggaat cgtcccgaaa taacagaaaa
      601 ggacatcgcc gctatcgtca ctgcccgttc tgcgccggaa gcgtttgctg tagtcaatgc
      661 gataatcgca gccgacgtag cgtccgcgct gaaagccaca ggttcggcga tggctgtccg
      721 tggggctgaa ttcggcttgc tgggacagct gcgatggaat atacagagat ctctgcaggc
      781 acagcagcga atatccggcg gagaaaatca gcaggcggtc ttccgttctt tgaggatttt
      841 caaggacagg gagtttgcgg cctatcttcg tcgccgcggc ttgcaaggcc ttcaaggcga
      901 tatgaaaaaa ctcctgcagg ctgacctgtc tatgaaatcc ggcacagaac caaaaacagc
      961 catgcagcaa ctcatagtag ctctctgcaa ttgaattttc cgatatgatt ttccgaaaac
     1021 gcgtagtgga aacaaggtag aaaaatgtca catataaaag ccgttctttc tgatatcggc
     1081 aacgttataa tgcccgtgca aactgagttt tttattaaaa atctcgtgga aaaatccggt
     1141 gcggatatga cggtggtaaa cgacgtcgtc aggaagacca tcgccgtcga acacgaaatc
     1201 aaccgcggcc gacgaaccat ccgccaggca ttcgacgaat tcatagccca accgctgaac
     1261 atcggctatg aagaatttct cgattcgtgg tatgccctgc tgggcgaaga gtatccgcag
     1321 gtgcgcgagg cgtatgagcg gctgaatgta ccgctgtacg tgctgagcaa cataaacgac
     1381 gttcacgctg actatctgca cggccggtgg ttcgaggaaa taagcatcgc atgctggatg
     1441 tccaacgaaa tcggcatgga caagcccagc cgcgaagtct ttgagttcgt actcggccaa
     1501 ataaaatgtc agccgtcaga actgctgttt ttcgacgata tcgcagaaaa cgtacaatca
     1561 gccgcttcgc tgggcataaa cgccgtgctc gtcaaaaatc ctgacgacgt ggtcaaaaaa
     1621 ctcgaagaat gcaagctgct cgtcggaaaa tcataactct gcgcgagttc acgaaaacat
     1681 ggacaactgc cgctccgatt cgtcacgctg ttccatgaat ttgccgacgg attttgcgcg
     1741 tagctgattt tcctgaatca gtcctgcctc gacaatatac agcggcgatt gttgatgtgt
     1801 gattttcgcc cgcaacgtaa gcggcatgcc gagttttatc ggatgcctga accgcacgtt
     1861 catctccgcc gtcaccgcca ctatgccgtg cgcaaaaaga cagttcgtca tcgccccgtc
     1921 caacaatgcc gacgtcacgc c
//
LOCUS       DAONFI010000385         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_80248_length_1941_cov_3.792153, whole genome shotgun sequence.
ACCESSION   DAONFI010000385 DAONFI010000000
VERSION     DAONFI010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80248_length_1941_cov_3.792153"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLK08_10055"
     CDS             complement(<1..730)
                     /locus_tag="PLK08_10055"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPS53883.1"
                     /translation="MNLGVQYYRPPFPVQRFWEDDFKKIADSGLNTVQLWILWSWVEP
                     KPGEFHFDDYDRLVELAEKNGLGIVLSTIAEVQPYWIHREVPGSEMIDHMGRKVVSSN
                     RGECHFGITPGGCIDNPGVWAGMKNFFNAAVSRYCKSPALRGWDAWNELRWNVNADGM
                     VCFCEHTLAAFRKWLSDKYGGLDGLNKAWIRRYGCFDEVMPGKMCGRPYTEMMAFEHF
                     LTWRANRHANLRYDFIKSIDPQHPV"
     gene            958..1749
                     /locus_tag="PLK08_10060"
     CDS             958..1749
                     /locus_tag="PLK08_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPS53884.1"
                     /translation="MTVAREFKFKPHKHKGFFEIDYVFEGVLTEEINNRKYVFPAGTL
                     VFIREKDTHSLYGSDLKLLNIVFSRKLMDTSISAAKLFNGIDVPYVTIPQKNRETFEA
                     ALNELFLKQGTPECELLSNRFFYDVLLEWFLPGRYDRIPAAPEWLRRGIEEMEKADVA
                     KWLVKDVVRKCCKSREHTARTFQAVFGCSISGYINRRRLEKAKKQLIYTNMSISDICF
                     DTGFNNVNYFYRLFKRFFFITPADYRRKNTHKSWAIITGKSTELY"
     gene            complement(1750..>1941)
                     /locus_tag="PLK08_10065"
     CDS             complement(1750..>1941)
                     /locus_tag="PLK08_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53885.1"
                     /translation="RNRAILRAGKEEKWQHTFLKNIFCRFSDAARLQKSRLQAAKVFG
                     IDSSYLRRNGSLFTEFFEN"
BASE COUNT          545 a          429 c          431 g          536 t
ORIGIN      
        1 gtactggatg ttgcgggtct atcgatttga tgaaatcgta gcggagattt gcatgacgat
       61 tggctcgcca tgtaaggaaa tgttcgaagg ccatcatctc ggtgtaaggg cggccgcaca
      121 tcttgcccgg catgacttca tcgaaacagc cgtacctgcg tatccacgcc ttgttcaagc
      181 cgtccaaccc gccgtattta tcggacagcc atttgcgaaa cgcggcaagc gtatgctcgc
      241 aaaagcagac catgccgtcc gcattgacgt tccatcgcaa ttcattccat gcgtcccagc
      301 cgcgtaatgc agggcttttg caatatcgac ttaccgcggc gttgaagaaa tttttcatgc
      361 cggcccagac gcctgggttg tcgatgcagc cgccaggcgt aatgccaaaa tgacattcgc
      421 cgcggttaga agagacgacc ttgcgaccca tgtggtctat catttcgctg cctggcactt
      481 cgcgatgtat ccaatatggc tgtacttcgg cgatggtgct gagcactatg cccaagccat
      541 ttttctctgc aagttctacc aggcggtcat aatcatcgaa atgaaattca ccaggctttg
      601 gttcgaccca cgaccatagt atccagagtt gtacggtatt caggccggag tcggctattt
      661 ttttgaaatc gtcttcccag aatcgctgta cgggaaacgg cgggcgataa tactgcactc
      721 ccaaattcat aattcctatt cctttcgaca gattttgctg aaagcctgcc attcagactg
      781 tgcagcgata aaaaattaaa cgaacatcat tttaaacagc ataaaattat atcattgccg
      841 taaaaaagcc tatagaatgg tattgacatt ttattgaacg tatgtgacat taaattaatg
      901 aaacataaaa acgtaacatg tttgaatttt gccagaataa gactgcccga atatcacatg
      961 acagttgccc gggaatttaa attcaagccg cataagcata aaggattttt tgaaatagat
     1021 tatgttttcg agggagttct tacggaagaa ataaataaca gaaaatatgt atttcctgca
     1081 gggaccctgg ttttcatacg cgagaaagac actcacagcc tgtacggttc agatttaaaa
     1141 ctgctgaata tcgtatttag ccgtaaactt atggatacct ccatatctgc cgcaaagctt
     1201 tttaatggaa tcgatgtgcc atatgtgact attcctcaaa aaaatcggga aacctttgaa
     1261 gctgctctca atgagctgtt tctcaagcag ggtacgcctg agtgcgaact tctatccaat
     1321 cggttttttt acgatgtgct gctggagtgg ttcttacctg gcagatacga ccgaattccg
     1381 gctgcgccgg agtggctcag gcgaggaatc gaagaaatgg aaaaggccga tgttgcaaag
     1441 tggctggtaa aagatgtggt aagaaaatgt tgtaaatcaa gagaacatac ggctcgtact
     1501 tttcaggctg tttttggctg ttcgatatct ggatatatca atcggagacg acttgagaag
     1561 gctaaaaaac agttgattta caccaacatg tccataagtg acatctgttt tgatacggga
     1621 ttcaataacg tcaattattt ctatcgactt tttaaaaggt tttttttcat aacgccggct
     1681 gattaccgac ggaaaaatac ccataagagt tgggccatca taacaggcaa atcgacggaa
     1741 ttatattgat taattctcga aaaattccgt gaataggctg ccgtttctcc tgagataact
     1801 agaatctatc ccaaaaacct tggctgcctg caatcggctc ttttgcagcc tggctgcgtc
     1861 agaaaaacgg cagaatatgt tttttaaaaa agtatgctgc catttttctt ccttgccagc
     1921 ccgcaaaatt gctcgatttc g
//
LOCUS       DAONFI010000386         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_80309_length_1940_cov_3.846154, whole genome shotgun sequence.
ACCESSION   DAONFI010000386 DAONFI010000000
VERSION     DAONFI010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80309_length_1940_cov_3.846154"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1335
                     /locus_tag="PLK08_10070"
     CDS             283..1335
                     /locus_tag="PLK08_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPS53886.1"
                     /translation="MIFESLLGMFSVDMGIDLGTCNTLVCVRGQGIVLNEPSVVAVKK
                     GTNRVLQNGNAVGIVAKEMLGKTPGSISAIRPMKDGVIADFDITEAMLSYFIRKVHRN
                     RSLIKPRVVIAIPSGITAVEKRAVLNSAERAGARKVYLVEEPMAAAIGAELPVTEPTG
                     SMIVDIGGGTTEVAIISLADISVCESIRVAGDDMDEAIANHMKRTYNLMIGEQTAERI
                     KIEIGSASPMETELTLEVRGRDMISGLPRKANISSEEVREALKEPVGAIIDCVTRTLE
                     KAEPELAADLVDNGITLAGGGGLLRGMDTVLANATGLDVRVAESPLTCVARGTAVYLE
                     NLEIWKDTMDSDEDEF"
     gene            1552..>1940
                     /locus_tag="PLK08_10075"
     CDS             1552..>1940
                     /locus_tag="PLK08_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53887.1"
                     /translation="MKTAPTIYLSSIFVLLALVLFGVAGCGGYYIMTVPDQVSRTGGD
                     VVPVARLQRNDFFILAIEVKDTPLVFRIDKGPERASYTDNMGYAGVELNVPRSPGKYN
                     LNVFLQDFEGEIVQQNVPVFVFDPSEPV"
BASE COUNT          466 a          458 c          543 g          473 t
ORIGIN      
        1 atcataaaat ttatcaacac tccagccagt gtccactcca tctgcggata acatcgacac
       61 tttttttcaa gaaccgctat aaaaccgtga aaagaatatt gtgaaacatt gatgccggcg
      121 gttgtccggg ttgtcggcat attttaaaat tccaactaaa aggccgattt cgctcaagac
      181 gaagtcggcc ttttgtcgat attatgggtg gtattatatc tttgcgcgca acgggcgtgg
      241 aaggaacgta ataggcctcc tattcaaacg ggaaggacga atatgatatt cgaatccttg
      301 ctgggaatgt tttcagttga catgggcata gacctgggca catgcaacac gctggtgtgt
      361 gtgcgcgggc agggtatcgt tctgaacgag ccttccgtag ttgccgtcaa aaaaggtact
      421 aaccgcgtat tgcagaacgg caatgcggtg ggcatcgtag ccaaggaaat gctgggcaag
      481 acgcccggct cgataagtgc gattcggccg atgaaagacg gcgtcattgc ggatttcgac
      541 ataaccgagg cgatgttaag ctatttcata cgcaaggttc accggaatcg ttcgctgata
      601 aagccccgcg tggtcatcgc cattccgtcg ggcataacag ccgttgaaaa acgtgccgtt
      661 ctgaattcgg ctgaacgtgc aggtgcccgt aaggtttatc ttgttgaaga gccgatggct
      721 gcggctatcg gtgcagagtt gcccgtgacc gagccgaccg gctcgatgat agtggatatc
      781 ggcggcggca cgacggaggt cgccataatt tcgctggcgg acatttccgt ctgcgaatcc
      841 atacgcgttg ccggcgatga catggacgaa gcaatcgcca atcacatgaa gcgcacctac
      901 aacctcatga ttggcgaaca gactgcggaa cgaatcaaga ttgaaatcgg ctcagccagc
      961 cctatggaga ctgaattgac gcttgaagtc agaggccgcg atatgatttc cggcctgccg
     1021 cgaaaggcta acatttcgtc tgaagaagtg cgggaagccc tcaaagagcc tgttggggcg
     1081 attatcgatt gtgttacccg tacccttgaa aaggctgagc cggaattagc cgccgacctt
     1141 gtggataacg gcattacctt agccggcggc ggcggactgc tgagaggcat ggataccgtg
     1201 ctggctaacg ccaccggcct ggacgtacga gtcgcggaat cgccgcttac ctgcgttgca
     1261 cgtggcacgg cggtttatct tgagaacctg gaaatctgga aagataccat ggatagcgac
     1321 gaggatgaat tttagcacgc tgtaacatgg aattcgtcgg gcaatagcag gtgctccgta
     1381 ccggcaggtc agatgcaaga gacgaaggcg gcggtatttt ttaaatcgga attcacggtt
     1441 ttttcttgcg gggcaagttc ccaatcatcc gtctgcacaa tacatcaaaa tcgatgtctt
     1501 tgtgccattt cttttgacat tgcaggcttg tgagtttaac ctgtagcaac tatgaaaaca
     1561 gccccaacga tatatttgtc gtcgattttc gtgttgctgg cacttgttct gtttggagtg
     1621 gccggctgcg gcggttatta cataatgacc gttccagacc aggtttcccg cactggcgga
     1681 gacgtagtgc ctgtggcacg tctgcagcgg aacgactttt tcatactggc gatagaggtc
     1741 aaggatacgc cgctggtttt ccgtatagat aaaggccctg agcgggcgtc gtataccgac
     1801 aatatgggtt atgcaggcgt ggaattgaat gtgccccgca gccctggcaa atataacctg
     1861 aacgtctttt tgcaggattt tgaaggtgaa atcgttcagc agaatgtgcc ggttttcgtt
     1921 ttcgacccct ctgagccggt
//
LOCUS       DAONFI010000387         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_81141_length_1928_cov_3.060331, whole genome shotgun sequence.
ACCESSION   DAONFI010000387 DAONFI010000000
VERSION     DAONFI010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81141_length_1928_cov_3.060331"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..993
                     /locus_tag="PLK08_10080"
     CDS             145..993
                     /locus_tag="PLK08_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPS53888.1"
                     /translation="MFEVIKNEVLADNLHRLVLKAPRIASARKPGQFVIIRSSTEAER
                     IPLTIADADAQAGTITIIVQAVGQGTREICDTPQGESLRDVAGPLGRPTHLEKWGRVA
                     CVGGGVGTAVLYPLAAGAAKLGNEVTTIIGARNASLVILKDELARFSKEVLIATEDGS
                     MGTKGFVTTVLGELIADESRRPHAIFAVGPVPMMAAVSEQTRPYGIPTFVSLNPIMVD
                     GTGMCGGCRVTVGGEIKFACVDGPEFDAHKVDFKELSSRQSTYRDEHFCRLDAKIEQL
                     RKEAGK"
     gene            990..>1928
                     /locus_tag="PLK08_10085"
     CDS             990..>1928
                     /locus_tag="PLK08_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPS53889.1"
                     /translation="MSENGLTPKERMAIPRTSMREQPASQRVHNFDEVNLGLLEEAAV
                     REANRCLECKNRPCVEGCPVSVRIPDFLSKLAKRDFAGAAEILLADNALPCTTGRVCP
                     QEKQCESKCVRGLKGDAVAIGWLERFVADWAAAHSARPTPPAKKTGWKVCVVGSGPGG
                     LTAAGELARIGHDVTVFEALHASGGVLRYGIPQFRLPKDIVDREVKNLGELGVKFEYN
                     VIVGKTVTLNQIMKEFDACFIANGAGLPMFLNVPGENLKGVYSANEYLTRVNLMGAYK
                     RGSRTPIVRGTKAVVFGGGNTAMDAVRTARRLGADPA"
BASE COUNT          439 a          533 c          575 g          381 t
ORIGIN      
        1 cgtaatatga aagtatccgc tgtgaggcgg acaggcgaac gcctgatgaa tatgctctaa
       61 cccgtagaga tttaatgaat tgcctgaaaa attgtaatct gatatttcca gccgccggct
      121 gtgagaagga gaaaaatttt aacgatgttc gaagtaatca agaatgaagt gcttgccgat
      181 aatttacatc ggctggtact caaagcccca cgaatagcat ctgcccgcaa gcctgggcag
      241 ttcgttatta tacgttccag taccgaggca gagcggatac cgctcaccat cgccgacgcc
      301 gacgctcaag ccggcacgat aaccataatc gtccaggctg tcggccaggg cactcgtgag
      361 atatgtgata cgccgcaggg cgaatctctg cgtgacgtcg caggccctct tggccggccg
      421 acccatcttg aaaagtgggg tcgcgtcgcc tgcgtcggcg gcggcgtcgg gacggcggtg
      481 ctttacccgc ttgccgccgg agcagccaaa ctcggcaacg aggtgactac aatcataggc
      541 gcccgcaacg cctcgctggt cattctcaaa gacgagcttg ccagattcag caaagaagtc
      601 ctcattgcca cggaagacgg gtcgatgggt actaaaggtt ttgtgacaac cgttcttggc
      661 gaactgatag ccgatgaaag ccgtcggccg cacgccatct ttgccgtcgg gcctgtgccg
      721 atgatggctg ccgtctcgga gcagacgagg ccttacggca ttccgacttt cgtgtcgctt
      781 aacccgataa tggttgacgg cacgggcatg tgcggcggct gtcgtgttac cgtcggcggc
      841 gaaatcaagt tcgcttgcgt cgacgggcct gaatttgacg cccacaaggt ggatttcaag
      901 gaactttcaa gccgtcagag cacatatcga gatgaacatt tctgccggtt ggacgcgaaa
      961 atagaacaac ttcgcaagga ggccggcaaa tgagcgaaaa cggacttacc ccaaaagaac
     1021 ggatggctat tccacgtacc agtatgcggg aacagcctgc ctcgcaacgc gtgcacaatt
     1081 tcgatgaagt gaacctcggc ctgctggaag aggctgccgt ccgtgaagcc aatcgttgtc
     1141 tggagtgcaa aaaccggcca tgcgtagagg gttgtccggt ttcggtccgg atacccgatt
     1201 ttctttcaaa gctggcgaag cgcgattttg ccggcgcggc tgagatactt ttggccgaca
     1261 acgccctgcc ctgcacgacc ggccgcgtct gcccgcagga aaaacagtgc gagtccaaat
     1321 gcgtcagagg ccttaaaggc gatgccgttg caatcggctg gctggagcgt tttgtagccg
     1381 actgggctgc ggctcattcc gccaggccga ctccgccggc aaagaagacc ggctggaaag
     1441 tctgcgtggt cggcagcggc cctggcggcc tgacggcagc cggcgaactt gcgagaatcg
     1501 gccatgacgt cacggttttt gaagcactgc acgcttccgg cggcgtattg aggtacggca
     1561 ttccgcagtt ccgtctgccg aaggatatcg tcgaccgcga agtgaaaaac ctcggcgaac
     1621 tgggcgttaa gttcgagtac aacgtaatcg tgggcaaaac ggtcacgctt aaccagataa
     1681 tgaaggaatt cgacgcatgc ttcatcgcca acggcgcagg cctgccgatg ttcctgaacg
     1741 ttcctggaga aaacctcaag ggcgtctatt cagccaatga atatctcacc cgcgtcaacc
     1801 tgatgggcgc atacaagcgc ggcagtcgca cgccgatagt tcgcggcacg aaggcggtgg
     1861 tattcggcgg cggcaatacg gcgatggatg cagtccggac ggccaggcgg ctcggcgcag
     1921 accccgcg
//
LOCUS       DAONFI010000388         1924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_81399_length_1924_cov_4.728197, whole genome shotgun sequence.
ACCESSION   DAONFI010000388 DAONFI010000000
VERSION     DAONFI010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81399_length_1924_cov_4.728197"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1717)
                     /locus_tag="PLK08_10090"
     CDS             complement(92..1717)
                     /locus_tag="PLK08_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPS53890.1"
                     /translation="MCCTFAGCVQPGKTDVDDVYRLQQSIVEHSPQKRGQAGLGPMIP
                     GYNGLHAIAPSGRTPEGKTVYSMSMEQVISYALANNTDVAVVSYNPSISREQVIQAAA
                     EFDYTIFGGYEYQNQNTSQRHYLDYDDAYSNDFTFGVKQKTITGADVSLSSTITRSLN
                     EGLVSNMGPWKNSTLDFSINQPLLRDAGPEVNLAKLNIARLSYKMSMAEFSQKAQEIV
                     VQTMSTYYQLIEVRKRVAVLWQLHKEAQDTYKKVLARRRMDATQVEISQSESAIQLRY
                     AALINAIKTAQDTQDQLVELIGDDTLNLTKKNIYIDTKTQWAVLPVKIDPEDQMLTAI
                     KYNPELEQARLAIEQGDIRVMVAKNQMLPKLDASFNISSDGGDPSSMSTALSQMSQGD
                     YLSYGAGIRFEYPIGNRERESFLREMKLKRRQSISTMQQKADAIALKINERIREVDKQ
                     YEEIVVRKRAVESITKQLRAITALEQIRKKMTPEFLQLKLNTQATLAEAKVQLLDSTL
                     QYNIALLELAKATGTVLELNKVQIATPPVLERN"
BASE COUNT          390 a          455 c          511 g          568 t
ORIGIN      
        1 gctcaggtta cgacgcagag aataatcgaa cggttttttc cggatatttt gtgagaactg
       61 ctctgacaaa agtgcgaggc aagggctgga gtcagtttct ttccaggacg ggcggagtcg
      121 ctatctgaac cttgttgagt tcgaggactg tgccggtggc ttttgcgagt tccagcaggg
      181 cgatattgta ttgaagcgtt gaatcaagaa gctgaacctt cgcttcagcc agcgtcgcct
      241 gggtgttgag tttcagttgg agaaattccg gcgtcatttt tttccgtatc tgttccaggg
      301 cggttatggc tctgagctgt tttgtgatgg attccaccgc ccgctttcga accacgattt
      361 cttcatattg cttgtcgact tcgcggatac gttcattgat tttcagtgct atggcgtcgg
      421 ctttctgctg cattgtggag atgctctggc ggcgtttgag tttcatttct cgtaaaaacg
      481 attcccgttc gcggttgccg attgggtatt cgaaacgtat gcctgcgccg tagctcaaat
      541 aatcgccctg cgacatctgc gataacgccg tactcatgga acttgggtcg ccgccgtctg
      601 aagagatatt gaacgatgcg tccagttttg gcaacatctg gttctttgcc accattaccc
      661 gaatgtctcc ctgttctata gccagccgtg cctgttcaag ttccggattg tattttatcg
      721 cagtcaacat ctggtcttca gggtctattt tgaccggcag aacggcccat tgcgtttttg
      781 tgtcgatata gatgtttttc tttgtcagat taagcgtgtc gtcgccgatt agttcgacga
      841 gctggtcctg tgtatcctgg gcggttttga tggcgttgat gagggctgcg tagcgaagct
      901 gtatggctga ttcggactgg gatatctcca cctgcgtggc gtccattctt cgtcttgcga
      961 ggactttttt gtacgtatcc tgcgcttcct tgtgaagttg ccacagaacg gccacccgtt
     1021 ttctgacctc gattaactga taataggtgc tcatggtctg gacgacgatt tcctgagcct
     1081 tctgactgaa ttcggccatg ctcattttat agctcagccg cgcgatattg agcttggcca
     1141 gatttacctc cggcccggcg tcgcggagca gcggctgatt tatgctgaaa tcaagcgtgc
     1201 tgtttttcca cggccccatg tttgatacca ggccttcgtt gaggctgcgt gttatcgtgg
     1261 atgaaaggct gacgtccgcg ccggttatgg ttttctgttt tacgccgaat gtaaaatcat
     1321 tcgaatatgc gtcgtcgtaa tccagataat gccgctgact ggtattctga ttttgatatt
     1381 cgtatccgcc gaatatcgtg taatcgaact cggcggcagc ctgtatgacc tgttcgcggc
     1441 ttatggacgg attatagctt accactgcga cgtctgtgtt gttggccagc gcgtagctga
     1501 tgacctgttc catcgacatg gaatagacgg tttttccttc cggcgtcctg cccgacggcg
     1561 cgatggcgtg caggccgtta tagcctggaa tcatcgggcc gagtccggcc tggcctcgtt
     1621 tttgcggcga atgttcaacg atggactgct gcagcctgta tacgtcgtcg acgtcggttt
     1681 tgcctggctg gacgcagccg gcaaatgtgc agcacaaaac agacgcaatt gcgccaaaag
     1741 agattaaatt gtaacggttc aggtacaaat acaccctttt tccgattccc gaaaccattc
     1801 ttaaaacaaa gattttaaaa aagttaggga actgatgcaa gattttagag cagttctctg
     1861 aagaaataat tttagaacct atcccaaaaa catgggcgtc gccgaaatcg agcaattttg
     1921 cggg
//
LOCUS       DAONFI010000389         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_81837_length_1918_cov_3.792807, whole genome shotgun sequence.
ACCESSION   DAONFI010000389 DAONFI010000000
VERSION     DAONFI010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81837_length_1918_cov_3.792807"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..>1918)
                     /gene="recG"
                     /locus_tag="PLK08_10095"
     CDS             complement(115..>1918)
                     /gene="recG"
                     /locus_tag="PLK08_10095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPS53891.1"
                     /translation="TISIRWFFGGYLAKTIQEGTVIAVSGKVCRYRGALQFINPGHQV
                     IYDPHGTSLDRDELMPVYPASGKLSSNIIAGIIKIVLPEVPRLIRPWFDKAFLAKRSL
                     MDRPTAITAMHNPDDREHWLQARRRLAYDECMLMQLGIALMRTREVSRPAHSLKWTPA
                     IDSHIRARFPFELTAAQNGAIKEIVSDLAKNHPMNRLVQGDVGSGKTVVAVYAALTAV
                     ANKKQVAIMAPTEILARQHYEKITRYLDGSQVRTALLVGAMPGNAADRIRAELANGDV
                     HIVVGTHALLGETVKFRELALVIVDEQHKFGVEQRTGLRGKGFAPHYLVMTATPIPRT
                     LAMTVFGDLDVSTIDSLPPGRGVTHTSCVTPSKFDEVIDFTANKIRQGQQAYFIYPLV
                     TPSPTLELQAAEQALKKLSAGPLGEFGVGMIHGQMKAEQKNDIMRAFAAGRIKILVAS
                     VVVEVGVDVSQANVIVINHAERFGLAQLHQLRGRVGRSADDANCILVASPNNPIAKRR
                     MEILCETSDGFKISEEDLRLRGPGEIFGTRQHGMPELKVADLVEDFDLLRLARRDAFK
                     IIEEDPDLAAPHNQQLRRMMLRMYAGKLTFITGA"
BASE COUNT          391 a          531 c          530 g          466 t
ORIGIN      
        1 cagccgacgc cgcaaacagc aacaagcgca aagtcaagtt taatatgctc taatggggaa
       61 aaactcgatg acatctacaa gagtggccgg ctgggcggaa attttgaaag ccggttatgc
      121 tccggttatg aaggtcaatt ttccggcgta cattcgcagc atcatacggc ggagttgctg
      181 attatgcgga gcggccaggt cggggtcttc ttctattatt ttgaaagcgt cccgcctcgc
      241 cagccgcagc agatcgaaat cttccacaag gtcagccacc ttcaattccg gcatgccgtg
      301 ctgacgtgtg ccgaaaatct ctccaggccc tctcagccgc aagtcttcct cggaaatttt
      361 aaatccgtct gacgtttcac acagaatttc catgcgtcgt tttgcaatgg ggttatttgg
      421 cgatgcgacg agtatgcaat ttgcgtcatc ggcgcttctg ccgacgcgac ccctgagctg
      481 atgaagctgg gctaagccga aacgttcggc gtggtttatt acaattacat ttgcctgcga
      541 cacatctacg ccgacttcga ccaccactga tgccacgaga atttttatgc ggccggccgc
      601 gaatgcccgc atgatatcgt ttttctgttc cgccttcatt tggccgtgaa tcattcccac
      661 gccgaattcg cccaatgggc cggcagagag ttttttcagc gcctgctcgg cggcttggag
      721 ttccagcgtc ggcgacggcg ttaccagcgg atagatgaag tatgcctgct gaccctgccg
      781 gattttgttt gctgtgaaat cgataacttc gtcaaatttt gacggcgtca cgcaactggt
      841 atgcgtaacg ccgcggccgg gcggcagaga atctatcgtc gaaacgtcca ggtcgccgaa
      901 aactgtcata gccagcgttc gcggtatcgg cgtcgccgtc atgaccaggt aatgcggcgc
      961 gaatcccttg ccgcgaaggc cggttcgctg ctccacgccg aacttgtgct gctcgtccac
     1021 tattacaagt gccagctcgc ggaatttcac cgtttcgccg agcagtgcat gagtgccgac
     1081 tacaatatgc acatctccat ttgcgagctc agccctgatg cggtcagccg cattgccggg
     1141 cattgcaccg accagcagtg ccgtgcgaac ctggctgccg tcgaggtatc gggtgatttt
     1201 ttcgtaatgc tggcgggcaa gaatttctgt tggcgccatt atggcgacct gctttttatt
     1261 cgctaccgca gtgagtgccg cataaaccgc gacgactgtt ttgccgctgc cgacgtcgcc
     1321 ctgtaccaac cggttcatcg ggtgattttt tgcaaggtcg gatactattt ccttaatggc
     1381 gccgttctgg gcagccgtca attcaaacgg gaatctggcg cggatgtgcg aatctatcgc
     1441 cggtgtccat ttaagcgagt gggccggcct ggaaacttcc cgcgtgcgca taagtgcgat
     1501 tccaagctgc atcaacatgc attcgtcgta ggctaaccgc cgccgcgcct gcagccaatg
     1561 ctcacggtcg tcgggattgt gcatggctgt tatcgcagtg ggcctgtcca tgaggcttcg
     1621 cttggcgagg aatgccttgt cgaaccaggg acgaatcagc cgcggcactt ccggcagaac
     1681 gattttgatt atgccggcta tgatattgct ggagagtttg ccgctagccg gatataccgg
     1741 catgagttca tcgcggtcga gagacgttcc gtgcgggtcg taaattacct gatgtccggg
     1801 attgatgaac tgcagcgcgc cgcgataacg gcagactttg cccgataccg ctatgaccgt
     1861 gccttcctga atcgtctttg cgagatatcc gccgaaaaac cagcggattg aaatggtc
//
LOCUS       DAONFI010000390         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_82550_length_1908_cov_3.256881, whole genome shotgun sequence.
ACCESSION   DAONFI010000390 DAONFI010000000
VERSION     DAONFI010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82550_length_1908_cov_3.256881"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLK08_10100"
     CDS             <1..229
                     /locus_tag="PLK08_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPS53892.1"
                     /translation="EIQQPTTWTIQFSAPVALFEEYMDKKLGRLGGQTWRANFYKCGD
                     KTSHPHWASWTEIGSELNFHLPERFGTIIFE"
     gene            386..1576
                     /locus_tag="PLK08_10105"
     CDS             386..1576
                     /locus_tag="PLK08_10105"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPS53893.1"
                     /translation="MKYAIIIPDGAADLPLDDLGGRTPFEAAKIPTIDSIVAMGRLGT
                     VRNVPDGYPAGSDVAIMSLLGYDPTEYYSGRAPLEAAAMGIKVTDDEWIFRCNLVTII
                     DDIMEDYSAGHISTEEASAIIAALQSTLNDTSVHLYPGVAYRHIMTVSGDMDVTTTPP
                     HDILGQGIARHLPRGEGSEVLLALMDRARYILADCDINAVRKDLGENPATDIWLWGQG
                     KMPKLDPFFQKYGVHGAAISAVDLVRGIARLMDWDVLTVEGATGYVDTNYEGKGRMAV
                     QALDHYDMVCVHVEGTDEAGHNADFKGKIHALEQIDKYIVAPVLERLKKEGDDWRILI
                     LPDHPTPCDVRTHTREAVPFVIAGRGIESGPAERKFTEAQAAQSDLHVEKGCNLMEYF
                     LTVY"
BASE COUNT          438 a          470 c          533 g          467 t
ORIGIN      
        1 tgaaatccag cagccgacta cctggaccat acagttttcc gctccggtag cgttatttga
       61 ggaatatatg gataaaaaac tcggccggct tggcggtcag acgtggcggg cgaattttta
      121 taaatgcggc gacaaaacgt cacatcctca ttgggcgtcg tggactgaaa tcggcagtga
      181 attgaatttt catctgcctg aaagattcgg cacgataatc tttgaatgac caggagcgtc
      241 tggcaaagtg ccgctgtatt cggccccttg ccgcgctgcc gcgttaaatt ttgttggaag
      301 atatagcggc cagcgcgtta aattttgtca aatagttttt tatggaaact tgtaagaacg
      361 ccctcgtgcg gatatcatgc cgcatatgaa gtatgcaatt ataattccgg acggggcggc
      421 agatttgccc ctggacgatc ttggcggcag aacgccgttt gaagctgcca aaattccgac
      481 aatcgattcg atagtggcca tgggccggct cggcacggtc aggaacgtgc ccgacggcta
      541 tcccgccggc agcgacgtcg ccatcatgtc ccttctgggc tacgacccga ccgaatatta
      601 ttctggcaga gctcctctgg aagccgccgc catgggcata aaagttaccg acgacgaatg
      661 gatattccgc tgtaacctgg tgacgattat cgacgacata atggaagatt attccgccgg
      721 ccatatttca acggaagagg cgtcggctat catcgccgct ttgcagagta cgctcaacga
      781 caccagcgtt cacctgtatc ccggcgtggc gtaccgtcat atcatgaccg tttccggcga
      841 tatggacgtt actaccactc cgccgcacga tattctcggc cagggtatcg ccaggcatct
      901 gccgcgcggc gaaggttcgg aagtgcttct tgcgcttatg gacagggcga ggtatatttt
      961 ggcggattgc gatatcaacg ccgttcgtaa agatttgggc gagaatccgg ctaccgacat
     1021 atggctgtgg ggccagggca aaatgccgaa gctcgacccg tttttccaga aatacggcgt
     1081 tcacggcgca gccatttcag cggttgatct tgtacgcggc atagcgcggc tgatggattg
     1141 ggacgtgctt accgtcgaag gcgcaacagg ttacgtggac acgaattacg agggcaaagg
     1201 ccgcatggcc gtacaggctc tcgaccatta cgacatggtt tgtgtgcatg ttgaaggtac
     1261 ggatgaagcc ggccataacg ccgatttcaa aggcaaaatc cacgcccttg agcagataga
     1321 caaatatata gtagcccctg ttcttgaacg tctgaaaaaa gagggcgacg actggcggat
     1381 tctgattctg cccgaccatc ctacgccgtg tgatgttcgc acgcataccc gtgaggctgt
     1441 gccgtttgtc atcgccggcc gcggcataga gagcggcccg gctgagcgga aattcaccga
     1501 ggctcaggct gcccagtctg atttgcatgt ggaaaaaggc tgcaacctca tggagtattt
     1561 tctcactgtg tactgatttt ccgtattaga gcgaagttgc agccagcaga aatgacgcat
     1621 ctcaaattta aaatgctcta agcgggaata tgcgatgttt gtgcattaat ccttgacttt
     1681 tcgggatttt gcgaatattt ttatcttggt tttctgtgaa attgtggata gcaggagagc
     1741 aattatgtct ttgaagtttt gggtgagatt gtcggcagta tgcggacttg ttgcattatt
     1801 ggctgttatg cccgtgatgg cggctgctcc tcagcctgac ccggcgaacg acggctggga
     1861 gttcgacatc cagtacggta tgccgcagag catactgctg acgctgcc
//
LOCUS       DAONFI010000391         1883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_84312_length_1883_cov_2.833151, whole genome shotgun sequence.
ACCESSION   DAONFI010000391 DAONFI010000000
VERSION     DAONFI010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84312_length_1883_cov_2.833151"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLK08_10110"
     CDS             <1..780
                     /locus_tag="PLK08_10110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPS53894.1"
                     /translation="RKYGLQKAVRYLTQKRSYYYALRHSAANAYISRYMCELANAQYP
                     PAKERNYPVQNGGLQCPFSDEEKDFEINRQRKPVIVAVQSDGEYKQSDLLLYMLREIK
                     SRMPDVPWKLIIIGTVYQPENFQNLIDELDCRENVELTGYLPSVRIHDYFRNAFCHVL
                     PSRLEGFGNTPLEAMAMGCPVVSSDCTAIPEVVGDAGILVPADDPKAFANGVMELYNN
                     PDLRKGCIKKGFMRVKEFSWTKSARLMYEIFCKAAGRKTGE"
     gene            781..1839
                     /locus_tag="PLK08_10115"
     CDS             781..1839
                     /locus_tag="PLK08_10115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPS53895.1"
                     /translation="MEQLTLLFTGDIYPGDIAVSPELLRKMSAADYRIGNLEGVMGRF
                     ELSEAQAGKTVLPCVSPGCVDFLKSVGLDYVALANNHMFDYGVAGFHGNVEILESAGI
                     SYSGAGENLAQARKPHIFEKNNIKTAFVSCAWNGTEARYATENSAGCAPLDEKWLMEM
                     PQKLKQDGIDHVILYVHWGYCGYSYPLPEHRELAMRLAGYGYAAVIGHHNHQMQGLLT
                     VDNSLIAFGLGDFVFAKYRTADGRIITQKGINSESAVLCLTLDKRGILSHDVIFTVQN
                     GDAVELDNRERQQKFFEQISKKLSVTSYERFWKYTVFKRLTLRVLHWINPFNWRHIKP
                     RTFNALVIMIKNIFGKKG"
BASE COUNT          515 a          378 c          493 g          497 t
ORIGIN      
        1 cgaaaatacg gacttcagaa agcagtgcga tatctgaccc agaaacgttc gtattactat
       61 gccttgcgac attctgcggc taatgcgtat atttcaagat atatgtgcga attggccaat
      121 gcccaatatc cgccggcgaa agaacgcaat tatcctgttc agaacggcgg attgcagtgt
      181 ccgttttccg acgaggaaaa agattttgag ataaatcgcc agcggaaacc ggtaatagtg
      241 gctgttcaga gcgatggcga atataaacag tccgacctgc ttttgtacat gctgcgggaa
      301 ataaaatccc gaatgccgga cgttccgtgg aagttgataa ttatcggcac ggtgtatcag
      361 ccggaaaatt ttcagaatct tatcgacgaa ctcgattgca gggaaaatgt cgaattgact
      421 ggttatctgc cgtcggtacg gatacacgat tattttagaa atgcgttctg tcatgttctg
      481 cccagccggc ttgaagggtt cggtaatacg ccgctggagg ctatggcgat gggctgtccg
      541 gtggtgtcga gcgattgtac ggctattccg gaagtcgtag gggatgctgg tattctggtg
      601 ccggctgacg accccaaagc gtttgccaat ggcgttatgg aactgtataa taatcctgat
      661 ttacgcaagg gatgtataaa aaaaggtttt atgcgcgtaa aagaattcag ttggacaaag
      721 tctgcgcggc tgatgtatga aattttctgc aaggcggcag gccgaaaaac aggcgaataa
      781 atggaacagt tgactttatt atttaccggc gatatatacc ctggcgacat agctgtatcg
      841 ccggaacttt tgcgaaaaat gtctgccgct gattacagaa tcggcaatct tgagggcgtg
      901 atgggccgat ttgaattgtc tgaggctcag gctggcaaga cggttttgcc gtgtgtgtcc
      961 cctgggtgcg tcgattttct gaaatcagtc ggcctggatt atgtggcgct ggcaaataat
     1021 cacatgttcg actacggcgt agcgggattt cacggtaatg tggaaattct tgaatcagcc
     1081 ggcatttcat attccggtgc cggtgaaaat ctcgcacagg ctcgcaaacc gcacattttt
     1141 gagaaaaaca atataaaaac ggctttcgtg agctgtgcct ggaacggcac tgaagcgcgg
     1201 tatgcgactg aaaattcagc cggctgtgcg ccgttggacg aaaaatggct gatggaaatg
     1261 ccgcaaaaac tcaaacagga cggtatcgac catgtgattc tgtacgttca ttggggctat
     1321 tgcggatatt catatccttt gcctgaacat cgggagcttg caatgcggct ggccggttat
     1381 ggatatgctg ccgtcatcgg ccaccataat catcaaatgc agggattgct gactgtcgac
     1441 aacagcctga ttgctttcgg gttgggcgat tttgtttttg caaaatacag aacggcagac
     1501 ggcagaatca taacgcagaa gggcattaac agtgaatctg cggttttatg cctgacgctt
     1561 gacaaacgcg ggatattatc gcatgacgtc atttttaccg ttcagaacgg tgatgccgtc
     1621 gaattggaca acagggaacg gcagcagaag ttttttgaac aaatatcaaa aaaattgtct
     1681 gtaacgtcgt atgagagatt ttggaaatat acggttttca aacggttgac attgagagtt
     1741 ctgcattgga taaacccgtt taactggaga catatcaaac ccagaacttt taatgcactg
     1801 gttataatga taaaaaatat ttttggcaaa aaaggttaaa acctatccca caatgggcgt
     1861 tgcctgaatg attaatatat ttt
//
LOCUS       DAONFI010000392         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_86229_length_1856_cov_3.556913, whole genome shotgun sequence.
ACCESSION   DAONFI010000392 DAONFI010000000
VERSION     DAONFI010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86229_length_1856_cov_3.556913"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1148)
                     /locus_tag="PLK08_10120"
     CDS             complement(87..1148)
                     /locus_tag="PLK08_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPS53896.1"
                     /translation="MTSKERVRAFFSHQPVDRVPIDALWNPGILHKTMEYFGVKTEIE
                     LKKSLGCDFQAVSVNYVGPKLHADITGKFVDSIFGWHTREIQHESGSYFDYCDFPLAN
                     AGVDEVAAWPIASPDDFDYDLLPARCEEYKDFGLYTGGPGLPDVINSAGCIRGVEQVL
                     IDLITDEPAGVLLFKRMADFKYQQLAKILEVIGDRIDFIWMGEDLGTQIAPMISMDVF
                     RKHILPVHKRFIDLAKKYNLPTMLHTCGSSSWAYEDFINAGLSGVDTLQPEATNMSPK
                     YLKQTFGGRLVFHGCISTTGKLTSGTPDEVREECRKTLEIMMPGGGYALCPSHQIQDN
                     TPLENVLAMYETANKYGWY"
     gene            complement(1295..>1856)
                     /locus_tag="PLK08_10125"
     CDS             complement(1295..>1856)
                     /locus_tag="PLK08_10125"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HPS53897.1"
                     /translation="GRIANGKVAGCAVLNDSKYGYSLDESTLTCTLIRSSYEPDPLPE
                     QGKFEMKFALMPHGRKISTGELIYTGNAFNQPLQVVATDIHKGHLPAVCDDFISCDSG
                     HVVITGIKKAEDEDAFIVRLLETSGKTAAAIVKVNSGFIAEIASAVEIDLLERPLPVS
                     SIKKLDNGFCVNVPANAIVNVKISIK"
BASE COUNT          508 a          476 c          353 g          519 t
ORIGIN      
        1 agcctcgtcc tcaacgacgc aacaaggcgc aatttcaaat ttaacatgct ctaatccatc
       61 cggacgccat tatgagagcg ggtttttcaa taccagccgt acttattggc ggtttcatac
      121 attgccaaca cattttccag cggcgtattg tcctgaatct gatgcgatgg gcacaaggcg
      181 tagccgccgc ccggcatcat tatttcaagc gttttacgac attcttctct aacttcatcc
      241 ggcgtaccgc tagtcaattt tccagttgtg cttatacagc cgtgaaaaac tagtcgcccg
      301 ccaaacgtct gtttcagata tttcggcgac atgttcgttg cctcaggctg aagcgtatct
      361 acgccggaaa gcccggcgtt gataaaatct tcatacgccc aactgcttga accacacgtg
      421 tgcaacattg tcggaagatt gtacttcttt gcgaggtcta tgaacctctt atgaaccggc
      481 agaatatgct ttcggaacac atccatgctt atcatcggag ctatttgcgt acccagatcc
      541 tcgcccatcc atatgaagtc aatcctgtca ccaataacct caagaatttt cgctaattgc
      601 tgatatttaa aatcagccat ccgcttaaaa agcagcactc cagccggctc atcggtaatg
      661 agatctataa gtacctgctc aacaccgcga atgcagccgg cggaatttat gacatccgga
      721 aggccaggac caccagtgta taacccaaaa tccttatatt cttcacaacg tgccggcaat
      781 aggtcataat cgaaatcatc cggcgacgcg ataggccatg cggcaacttc atcaacgccg
      841 gcatttgcaa gaggaaaatc acaatagtca aaataactgc ctgattcatg ttgaatttca
      901 cgtgtatgcc agccaaaaat cgaatccaca aatttcccgg taatatcggc gtgcagtttt
      961 gggccaacat aattgacact taccgcctga aaatcacagc ctaaagattt tttaagctct
     1021 atctccgttt ttacgccaaa atactccatt gtcttgtgca aaattccagg attccaaagt
     1081 gcatctatcg gcacacggtc aaccggttga tgactgaaaa acgcacgtac gcgttcttta
     1141 gaagtcatgg tctccctgcc agtaaccaat gtttttgcag acatactttt cctttcaaac
     1201 aaattttcaa atacggaatc agacatttta ctgcataaca gaataaaact acatcgaact
     1261 cagactacca gatttggggg tataatttca atttttattt tatgctaatt ttcacattca
     1321 ctatcgcatt cgccggtaca ttgacacaaa aaccgttatc gagttttttg atactactga
     1381 caggcaatgg cctttcaagc aaatcaattt ctactgctga tgcaatttca gctataaatc
     1441 cagaatttac tttcacaatc gccgccgcag ttttgccgga tgtctccaac aaccgtacta
     1501 taaatgcatc ttcatcttcc gccttcttta taccggttat gacaacgtgt ccggaatcgc
     1561 atgaaatgaa atcatcacat acggcaggca gatggccttt atgaatatcg gtagccacaa
     1621 cctgcaacgg ctgattaaac gcatttccgg tatatataag ctcacctgtt gatatttttc
     1681 tgccgtgcgg catgagtgcg aatttcatct caaatttgcc ttgttcaggc aacgggtccg
     1741 gttcgtaact cgaccgtatc aatgtgcacg tcagtgtcga ttcatccaga gagtatccgt
     1801 atttactgtc gttgagcact gcgcagccgg cgacttttcc attggcgatt ctgcct
//
LOCUS       DAONFI010000393         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_88593_length_1826_cov_2.961039, whole genome shotgun sequence.
ACCESSION   DAONFI010000393 DAONFI010000000
VERSION     DAONFI010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88593_length_1826_cov_2.961039"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLK08_10130"
     CDS             <1..1114
                     /locus_tag="PLK08_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HPS53898.1"
                     /translation="TGMFEWLATLIAKAVRGNALLIFMLFLIITAVISSVLDNVTTVI
                     LIAPITILVCQVLELPAIPFLVFEALFSNIGGTATLVGDPPNVIIGSQAKLSFNEFIM
                     HLTPVIVIVMIISLAVVYLWFGRKFKVRQSAREQIMKTNPSLAIIDRNNMIKGLCIFA
                     LVLAGFFLGRVLDIEPGIVALAGGMLMALVCRVEIHRVLGKVEWNTIMFFVGLFILIG
                     SLEVNHVFEKMGQGMLWLTNGNLLMTVIVLLWFSAIASAIVDNIPLVIAMIPLIKSII
                     PDFAHNMGLDVASHQAQILISDPLYWSLALGACLGGNGTMVGASANVVICQIAKRNNY
                     NITFMGFTKLGMPMMVLSLVICTCYLWLRYFVFNHV"
     gene            1175..>1826
                     /locus_tag="PLK08_10135"
     CDS             1175..>1826
                     /locus_tag="PLK08_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPS53899.1"
                     /translation="MKVYLETLGCQMNRLDSELIGGMLRAEGYSFTDDPRSADVLLYN
                     TCSVRDHAEQKVYSRLGADARRKSAGKKLIVGVLGCMAQKDREGLARYPAVDIICAPG
                     QIYRLCDMLAQAASSKGLQIALDPKRNEPVDEAARQAIDEMDILRDISVTPSRSQAFV
                     RVMRGCDNFCTYCIVPYTRGPEASRSPHDVYEEVRRLVDAGRSEITLLGQTVNSYHH"
BASE COUNT          396 a          419 c          497 g          514 t
ORIGIN      
        1 gactggcatg ttcgaatggc tggcgacgct tatcgccaaa gcggtgcgcg gcaatgcgtt
       61 gctgattttc atgctgtttc tcataatcac cgccgtcatt tcttcggtat tggataacgt
      121 cacgaccgtc atactgattg ctccgattac aatacttgtc tgccaggtgc tcgaactgcc
      181 ggcgataccg tttctggtat tcgaggcgct gttttcaaat atcggcggta cggcgacgct
      241 tgttggtgac ccgccgaacg ttattatcgg ttcgcaggct aaattgagct tcaatgaatt
      301 cataatgcac cttacgccgg taatagtaat tgtgatgata atttcccttg cggtagtgta
      361 tctatggttt ggccgcaagt tcaaggtgcg acagagcgct cgcgaacaga taatgaaaac
      421 caatccgtca ctggcgatta tcgaccgcaa caacatgata aaaggcctgt gcatttttgc
      481 tctggtgctg gctggatttt tcctcggcag agtgcttgat attgaaccgg gtatagttgc
      541 attggccggc ggcatgctta tggcgctggt ctgtcgggtg gagattcatc gcgtgctggg
      601 caaagtcgag tggaacacca taatgttttt cgtcgggctg ttcattctca tcggctcgct
      661 tgaagtgaat cacgtgttcg aaaaaatggg acagggaatg ctgtggctga cgaacggcaa
      721 tcttcttatg acggtaatag ttctgttatg gttcagtgcg attgcgtcgg ctattgtgga
      781 caatattccg ctggtaattg ccatgatacc actcatcaaa tccataatac ccgatttcgc
      841 gcataatatg ggactcgatg tcgcttcgca tcaggcgcag attctgatat ccgatccgtt
      901 gtactggtcg cttgctcttg gggcgtgttt gggcggcaat ggtacgatgg ttggtgcgtc
      961 ggctaacgtc gtcatatgtc agatagccaa acgcaacaac tataacataa cgtttatggg
     1021 tttcacgaaa ctcggcatgc ccatgatggt gcttagcctt gttatatgta cctgctattt
     1081 gtggttgcgt tatttcgtgt tcaaccacgt gtaagacgga ctgcgaaatg agttttcatt
     1141 ttttgatttt tttgcgtaca atatccgtct tgccatgaaa gtatatcttg aaacgttggg
     1201 ttgtcagatg aatcggctcg atagtgagct gataggcggc atgttgcgcg cggagggtta
     1261 ttcctttacg gatgacccgc ggtcagctga cgtgctgtta tataacacct gttccgttcg
     1321 cgaccatgcc gaacagaagg tctattccag gctcggtgcc gatgctcgca ggaaatcggc
     1381 gggtaaaaaa ctgatagtcg gcgttctggg ctgtatggcg caaaaggatc gtgaagggtt
     1441 ggccaggtat ccggcagtgg atattatttg tgcgccaggg cagatttaca ggctgtgcga
     1501 tatgcttgcg caggcagctt catcgaaagg ccttcagatt gcacttgacc caaagcgcaa
     1561 cgaacccgtc gacgaggctg ccaggcaagc cattgatgag atggatattt tacgcgatat
     1621 ttctgttacg cccagccgca gccaggcgtt tgttcgcgtt atgcgcggct gcgacaattt
     1681 ttgcacttac tgtattgtgc cgtatacccg cggccctgaa gccagccgtt cgccgcacga
     1741 cgtttatgag gaggttcgcc gtctcgtcga tgccggccga agtgaaataa cccttcttgg
     1801 ccagacggtc aacagctatc atcatt
//
LOCUS       DAONFI010000394         1825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_88658_length_1825_cov_3.164407, whole genome shotgun sequence.
ACCESSION   DAONFI010000394 DAONFI010000000
VERSION     DAONFI010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88658_length_1825_cov_3.164407"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLK08_10140"
     CDS             <1..1118
                     /locus_tag="PLK08_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53900.1"
                     /translation="GLNVASEVAMKETQDLVNNTDYIKVGGMQSGLVPETKLLVTKIM
                     EKEKAIQQNSDTITSLTKQLSAKEEDIKKIQDDAKASLDKLEGQFENLNKMYQDDHAG
                     FAEKAKEFSDNLSDARKTLRNDAKKYEQKIEKLTLDNHSLKREITKLNAQIDDLNERI
                     HGGGKTTRKADGIVSRVVPSSGLCYINLGSENNVQDGMTFAVFGPNDDNAAAPKAKLR
                     AINVSPTFSECQVVESTDGKQVEDNDVVTNLVYDPNRKYVFVVKGVFDLYGDGRASQD
                     QTNEVIQAIKLHGGEIGKEVNVQTDYVVMGVEPTKPIKPSDDAPDSVQLAYKKQMQAY
                     DDYFKTKKAAEGLKIPVLNTNRFLDLTGYMPEKRPQE"
     gene            1223..>1825
                     /locus_tag="PLK08_10145"
     CDS             1223..>1825
                     /locus_tag="PLK08_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPS53901.1"
                     /translation="MKLSQRIQNVSASPTIAITAKAKQMKNEGIDVVSFGAGEPDFDT
                     PAFIVDACIAALKSGDTRYVPQKAASLKNAIAAKLEKENHITVDPSQVVVTFGGKHAL
                     YDAFQAVINPGDKVLLPAPYWVSYPEQIKLAGGEVVFLETGPEHNFKITPKQILDNAD
                     AKVLVINSPSNPSGLMYTPDELKAIADAVLKTDMMVFSDEI"
BASE COUNT          576 a          463 c          440 g          346 t
ORIGIN      
        1 ccggcctcaa tgtcgcttcg gaagtcgcga tgaaagagac ccaggacctc gtcaacaaca
       61 ccgattatat caaagtcggc ggcatgcaga gcggactggt gcctgaaaca aaactccttg
      121 tcaccaaaat aatggaaaag gaaaaggcta tccagcagaa ttcagacacc ataaccagtc
      181 tgaccaagca gctctccgca aaggaagagg atatcaaaaa aattcaggac gatgcaaaag
      241 catcactgga caaactcgaa ggccagtttg agaatctgaa caagatgtat caggacgatc
      301 atgccggctt cgccgaaaag gctaaagagt tcagtgacaa cctctccgac gcccgcaaaa
      361 cactccgcaa cgatgcaaag aaatatgaac agaaaattga aaaactcacg ctcgacaatc
      421 acagcctcaa gcgtgaaata accaaactca atgcacagat agatgacctc aacgaacgaa
      481 tccacggcgg cgggaagacc actcgcaaag ccgacggcat agtcagccgg gtcgttcctt
      541 cgtcgggcct gtgctatatc aacctcggca gtgaaaacaa tgtgcaggac ggcatgactt
      601 ttgcggtctt cgggcctaat gacgacaacg ccgcagcccc gaaagccaaa ctgcgtgcga
      661 taaacgtctc ccccacattt tcagaatgcc aggtggttga aagtaccgac ggcaaacagg
      721 tggaagacaa tgatgtcgta acgaatctcg tatatgaccc gaatcgcaag tacgttttcg
      781 tagtcaaggg cgtattcgat ctttacggtg acggcagagc ttcacaggat cagacgaatg
      841 aagtgattca ggctatcaag ctgcacggcg gcgaaatcgg caaggaagtc aacgtccaga
      901 ccgattacgt agtcatgggc gtagagccga caaaaccgat aaaaccatca gacgacgctc
      961 ctgattccgt ccagcttgcg tataaaaaac agatgcaggc atacgacgat tattttaaaa
     1021 caaaaaaagc ggctgaaggg ctcaagatac ctgtcctgaa tacgaacaga tttcttgatc
     1081 tgaccggtta catgcctgag aaacgcccac aggaataagg gtaaaactga atggaaaata
     1141 aaaggcggtt ccggcggaca agtcggagcc gcctttttta cggctacttt tcagaaaagg
     1201 aatttttgag gaatcgaaaa taatgaaact atcgcaaaga atccagaacg tatcagcaag
     1261 tccgaccatc gccataaccg cgaaggccaa gcagatgaaa aacgaaggca ttgacgtagt
     1321 gagtttcggc gcaggcgagc cggattttga cactccggca tttattgtcg atgcatgcat
     1381 cgcggcgtta aaatcaggcg acacccgtta cgtgccacaa aaagcagcca gcctgaagaa
     1441 cgccatcgcc gccaagctcg aaaaagagaa tcacattacg gtcgacccat ctcaggtggt
     1501 tgtgactttc ggcggcaaac acgcgttata cgatgccttt caggctgtca taaaccctgg
     1561 cgacaaagtc ctgctgccag ccccatactg ggtcagttac cctgagcaga taaagctcgc
     1621 cggcggcgaa gtggtgtttc tggaaacagg ccctgaacat aatttcaaaa taacgccgaa
     1681 acagattctg gataatgccg atgcaaaagt gctcgtcata aacagccctt caaatccatc
     1741 cggcctcatg tacacgccgg acgaactcaa agccattgcg gacgccgttc tgaagaccga
     1801 catgatggta ttttcggacg aaatt
//
LOCUS       DAONFI010000395         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_89332_length_1816_cov_3.833617, whole genome shotgun sequence.
ACCESSION   DAONFI010000395 DAONFI010000000
VERSION     DAONFI010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89332_length_1816_cov_3.833617"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..854)
                     /locus_tag="PLK08_10150"
     CDS             complement(81..854)
                     /locus_tag="PLK08_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPS53902.1"
                     /translation="MNFGDILRKRREELGLTQDALALLTGISKPYLSNIETGRAKNPP
                     TDGVLSELEKHLQMAAGELTRLAHLARTPCDVLQEHETLSAENVKLKKMLSQLLKESP
                     DQRAAQFQEVLGRDENFPAISAGIVVPVINRVAAGYPQAFTDLDYPVSVADEYVRCPD
                     IRDQQAFATRVVGDSMEPKYHEGDIVVFSPNTQPRPGDDCFVRFGYDEGTTFKRFYQD
                     SPESVRLQPLNSKYPSVTYPTESITGIWPAVFRIESLRR"
     gene            1205..1639
                     /locus_tag="PLK08_10155"
     CDS             1205..1639
                     /locus_tag="PLK08_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53903.1"
                     /translation="MPVSLRETIARKEDLPRTDSVLMGRAELLSPADRDLLEAVLVRS
                     QPVAALGRIMNVDARALRTRIHRLGRRLSSRRFLDAARALPYLDNDSARLARLHFCQK
                     ISVVDLAGLEGISRYTMRRRMERIAAQIQAVRRVSRIRNQSF"
BASE COUNT          436 a          427 c          479 g          474 t
ORIGIN      
        1 ttccgatgca gcctgacgca gcagagcgat gttgcagcca gcagaaatga cgcatctcaa
       61 gttaaaatgc tctaaagcac tcatcgtctc agtgattcta tcctgaaaac tgccggccag
      121 atgccggtta tggattccgt cgggtaagtt acggaaggat atttgctgtt cagcggctga
      181 aggcggactg attctggcga atcctgataa aaacgtttaa aagtcgtgcc ctcatcgtag
      241 ccgaatcgca cgaagcagtc gtcgccgggc cgcggctgag tgttgggcga aaaaactacg
      301 atgtcgcctt cgtggtattt gggctccatg gaatcgccga cgacccgcgt ggcgaaggcc
      361 tgctggtcgc gtatgtcggg acagcggacg tattcgtcgg ctacgctcac ggggtagtcc
      421 aggtctgtaa atgcctgcgg atagccggct gctacacgat ttatgaccgg cacgactatg
      481 ccggctgaaa tagccggaaa attctcatcc cggcccagga cttcctgaaa ttgggcggct
      541 cgctggtcag gcgattcttt cagcagttgg ctaagcattt tcttgagctt gacgttttca
      601 gccgacaatg tttcgtgttc ctgcaagacg tcgcacggcg ttcgcgccag atgagccagg
      661 cgggtcagct cgccggctgc catctgcaga tgtttctcaa gttccgacaa tacgccgtcg
      721 gtagggggat tttttgcgcg gcctgtctcg atattggaga ggtacggctt gcttatgccg
      781 gtaagcaggg caagcgcatc ctgcgtcagg cccagctctt ctcgccgctt acgtaaaatg
      841 tcgccaaaat tcatcttgca cctccatgca cccgccgccg gcggaaattt ttttataaca
      901 acacaacaac gttgcctgca aatgctgaaa aatatttttt taagttttta tgtaagagca
      961 atacataaaa acgcggctgt taatgaaagg cattttactc caattaccca aaaaaagttc
     1021 cgattatgct gaattataga cgttgttaat caaaaagtca attttcattc tttaaatgtt
     1081 aattaaaaaa caaatttttt atttcgcttg actttgtatg ttttctgatt atcatacata
     1141 aaagtatcat ttgtgttttt catacaagaa tagggtaaca gggaaaagca taggaggtag
     1201 atttatgccg gtttctctac gcgagactat agcgaggaag gaagaccttc cccgtacgga
     1261 ttcagttcta atgggccgag ccgagctttt atcgccagct gaccgcgact tgttggaggc
     1321 ggttttggtt cggtctcagc cggtagctgc gttgggccga ataatgaacg ttgatgcgcg
     1381 tgcgttacgg acgcgcattc acaggctggg ccggcggctg tcgagtcggc gtttcctgga
     1441 tgccgcccgc gcgctgccat atctcgataa cgattctgct aggctggccc ggctgcattt
     1501 ttgtcaaaaa atatccgttg tcgacctggc cggcctggaa ggcatatcgc gatatacgat
     1561 gcgccgccgc atggagcgga tagccgcaca gatacaggct gtccgacgcg tcagccgtat
     1621 caggaatcaa tcattctgag gacgatggcg aatttcaaac tgctcttgaa aaacggcgga
     1681 atcatttttt ttaaaaaaaa acggttttgg aaaatttttg ggattttcag ggagagttac
     1741 aatgactgca tgtttacata cgaatcaatg gcggacgaat gtggattttg aaatcgtcgg
     1801 ccgattgccg tcgagc
//
LOCUS       DAONFI010000396         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_90523_length_1801_cov_3.621420, whole genome shotgun sequence.
ACCESSION   DAONFI010000396 DAONFI010000000
VERSION     DAONFI010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90523_length_1801_cov_3.621420"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PLK08_10160"
     CDS             complement(<1..1269)
                     /locus_tag="PLK08_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPS53904.1"
                     /translation="METLREQYVPSATESYFAGFREVIRAGWPASLTMLNSTIIAFTD
                     GLMVSRVNAVSLNAQFAAGISSFVFEAFFVGVLGIVNTFVSQNYGSRRYDMTSQYVHA
                     GMAISVMASLLFAPLAFFPEPFFRLMSSDYADAGQFVSLATMYFRYMMLTLVLRLLTT
                     VLCQFFYGVQRQRVVLLASMLAMVVNVVGNYIFIFGHLGFRPMGLEGAAWGSVLAFAS
                     QFVLLGAIFLSGNYNRLYQTRRHWRISWKCVRDIMSVGWPAGVQFFSDIFCWMIFTGF
                     LVGKKFGPEHLTGTVIAMRYMNVSFMPAIGIGIATTSLVGKYIGAKRPELVRRRARQG
                     CMLAVVYMGVCGLLFWIFGRQMAEFYVQQDVLAGGATAGNAEAVINISRRLLMCAAAF
                     QIFDGIGIVYSGALQGAGDTWWKMLCAIILT"
     gene            complement(1271..>1801)
                     /locus_tag="PLK08_10165"
     CDS             complement(1271..>1801)
                     /locus_tag="PLK08_10165"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPS53905.1"
                     /translation="YWVRHVADSLSVGRVCPEIFSADCGLKIADVGCGGGFPILPLAC
                     VNPQLDITGIECRGKKTQFISEAARELGLPNVKFVTAQAREVGRAEGHAGVYDVILMR
                     AVGESGRMLREVRNLVAAGGRVINYKTPQAVAGERLILAREAGKFGFDIVESMPFELP
                     CGGGLRQFVILNRNNG"
BASE COUNT          479 a          559 c          432 g          331 t
ORIGIN      
        1 cgtaagaatt atcgcacaca gcatcttcca ccacgtgtcg cccgctccct gaagcgcgcc
       61 gctgtacact attcctatgc cgtcgaatat ctggaacgca gccgcacaca taagcagccg
      121 gcggctgatg tttatgaccg cctcggcatt gccggcagtc gcaccgccgg caagcacgtc
      181 ctgctgcacg taaaactccg ccatctgtcg gccgaaaatc cagaacagaa ggccgcacac
      241 tcccatataa accacggcaa gcatgcaacc ctggcgcgca cgccttctga ccagctcagg
      301 acgcttcgcg ccgatgtatt tcccgaccag cgacgtcgtg gcaatgccga ttccaatcgc
      361 cggcataaaa gaaacgttca tataccgcat cgcaataaca gtaccagtca gatgttcagg
      421 gccgaatttt ttgccgacga gaaagcccgt aaaaatcatc cagcagaaga tatcgctgaa
      481 aaactgcacg ccagccggcc agcctacaga catgatgtcg cgaacgcact tccacgaaat
      541 ccgccagtga cggcgtgtct ggtagagccg attataattg ccggataaaa atatcgcgcc
      601 gaggagcacg aactgcgacg cgaatgcaag tacagacccc cacgccgcgc cttccagccc
      661 catcggccga aaacccagat gaccgaaaat gaatatgtaa ttgcccacga cgttgacaac
      721 catcgccagc atcgaagcca gcaaaacaac cctctgccgc tgtacgccgt agaaaaactg
      781 acagagcacc gtcgtcagca gccgcaacac aagcgtcaac atcatgtatc tgaaatacat
      841 cgtggcgagt gaaacaaact ggcctgcgtc ggcataatcg ctgctcatca ggcgaaagaa
      901 cggctcgggg aaaaaggcca gcggcgcaaa aagcagagat gccataaccg atatcgccat
      961 gccggcatga acgtactgcg acgtcatgtc gtatcgccgt gagccgtaat tctggctgac
     1021 gaaagtattg acgattccca aaacacctac gaaaaatgcc tcgaacacaa acgaagatat
     1081 gccggcggca aactgggcgt taagtgaaac ggcattaacc cgcgacacca tcaagccgtc
     1141 cgtgaatgct attatcgtac tgttgagcat cgtaaggcta gccggccagc cggcccttat
     1201 gacttcccta aaaccggcga aataactttc cgtcgccgac gggacgtatt gttcccgcaa
     1261 tgtttccata tcaaccgtta ttcctgttta atatcacaaa ctgtctcaat ccgccgccgc
     1321 acggcaattc aaacggcatg gactcgacga tatcgaaccc gaatttgccg gcttccctcg
     1381 caagtatcag ccgttcgcca gcaacagcct gtggagtttt gtagtttatc acccgaccgc
     1441 cggctgcaac gagatttcgg acttcgcgca acatacggcc gctctcgccg acggcgcgca
     1501 tgaggatgac gtcgtatacg ccggcatggc cttcggcacg gccgacttca cgagcctggg
     1561 ctgttacgaa cttcacgttc ggcagaccaa gttcgcgggc agcctcggaa atgaactgag
     1621 tcttctttcc gcgacactcg atgcctgtta tgtcaagctg cggattgacg cacgcaagcg
     1681 gcagaatcgg aaatccgccg ccgcagccga cgtcggcaat ttttaatccg caatcagcgg
     1741 aaaaaatttc aggacagacc cgccccaccg acaacgaatc cgccacatga cgtacccagt
     1801 a
//
LOCUS       DAONFI010000397         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_90526_length_1801_cov_3.548683, whole genome shotgun sequence.
ACCESSION   DAONFI010000397 DAONFI010000000
VERSION     DAONFI010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90526_length_1801_cov_3.548683"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1801
                     /locus_tag="PLK08_10170"
     CDS             <1..>1801
                     /locus_tag="PLK08_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53906.1"
                     /translation="MRPVLTDKMAVVIVLAGLVAWLCTMGILWGWAYDTSGEITMRWL
                     FMVALCVTAALYKRHKIAHLTLRLIRGMRIFSRIPLEDSFGGNLGSSRQDVQKRTSLM
                     AISIVTAVSCFIFSTIWIILARLVGTLIKSYFLFWDWTWSNIENFFLFIGAIPVAAGL
                     FAYFYASKVVRESSGRDVYAGCYRDWIWSAALGFFIFALSWWFGANLIVLVFSIAGVL
                     LVGAFTEISRMEMATHPRKAMLPFGPPAPQTQWMLTLLHAGLTLVLIAQCRMLSDIFS
                     LSMTRRCLWLFMSLGLLGYFLGRVDRKGKTLSSLQTTGAVIGLGAGLLGQATELILCN
                     YLKVGVGITVFFAIATQIALASLAAELISHQRRTFAVSGGTIGGFLLAVAGGITGGIT
                     IFGLLTWLPATGKIAGAIPIAMIIAGSYFGVKHLRKDDHRKPYIIWCSLLAVSFTWGL
                     IDSCRKAAANIDGEVSIGSWLTSIVKDTPKAKTHVQNGVLPANAVKCNEIITRCLYEA
                     REDKDGKIMEDGLFSHRRGKWWVVATDAADMPHDTPYRFYAFGSNPQPPHVAKKLKHR
                     YRPLSYSCPDYLEYASLNFTAGLGCDFYDGIFLA"
BASE COUNT          392 a          492 c          483 g          434 t
ORIGIN      
        1 atgcgtccgg tacttactga caaaatggct gtcgtaatcg tgctagccgg cctggtggca
       61 tggctgtgca caatgggaat tttgtggggc tgggcatacg acacttctgg cgaaataacc
      121 atgagatggc tgttcatggt ggcgttgtgc gtgacggcgg cactttataa acgtcacaaa
      181 attgcacacc tgacgttgcg actcattcgc ggcatgagaa ttttttcccg tataccgctg
      241 gaggattcct tcggcggcaa tctcggctca agccggcagg acgtccagaa acgcacctcg
      301 ctgatggcga tttcaatagt aacggctgtg agctgtttca tattctcgac aatatggata
      361 attttggcca gactcgtcgg cacactgata aaatcatatt tcctgttctg ggactggacg
      421 tggtccaata tagagaactt ttttctgttc atcggcgcca tacccgtggc agccggcctt
      481 tttgcatatt tttatgcgtc aaaggtggtt cgtgagtcgt caggccgtga cgtatacgca
      541 ggctgttacc gcgactggat atggtctgcg gcgctgggct ttttcatttt cgcattgtcg
      601 tggtggttcg gcgcaaatct catagtcctg gttttcagca tagccggcgt tctgctggtc
      661 ggcgcattta ccgaaataag ccgcatggaa atggccaccc acccgcgtaa agccatgctc
      721 cccttcggac ctccagcccc gcaaacgcag tggatgctca cccttctcca cgccggcctt
      781 acgctggtcc tcatcgcaca atgccgcatg ctttcggaca ttttctcgct gagcatgacg
      841 aggcgatgcc tgtggttatt catgtcgctg ggtctgctgg gttatttttt gggcagagtc
      901 gaccgcaagg gcaagacgct ttcatcgctt cagacgaccg gcgcagtcat aggccttggc
      961 gccggccttc tcggccaggc gacggaactg attttatgca actatctgaa ggtaggagtc
     1021 ggcataacgg tatttttcgc catcgccaca cagatagcgc tagccagcct ggctgctgaa
     1081 ctcatctcgc accagcgacg gacttttgca gtttccggcg gaacaatcgg cggatttctg
     1141 ttggctgtgg caggcggcat tacgggaggc attacgattt tcggcctgct gacatggctg
     1201 ccggctacgg ggaaaatcgc cggcgctatc ccgatagcca tgattatcgc cggcagttac
     1261 ttcggcgtca aacatctgcg aaaagacgac cacaggaaac cttatataat atggtgcagc
     1321 ctgctggctg tcagtttcac atggggactt atcgacagct gccgcaaagc ggcggcaaat
     1381 atcgacggcg aagtgtccat cggttcatgg ctgacgtcga tagtaaaaga cacccccaaa
     1441 gccaaaaccc acgtccagaa cggcgtactg ccggcaaacg ccgtcaaatg caacgaaatt
     1501 ataacccgct gtctttacga agctcgcgaa gataaggacg gcaaaataat ggaagatggc
     1561 ctgttttcgc atcggcgagg caaatggtgg gtcgtagcca ccgacgccgc cgatatgccg
     1621 catgatacgc cctaccgctt ttacgcattc ggctcaaatc ctcagccgcc gcacgtggca
     1681 aaaaaactga aacaccgcta ccggccgctg agctattcct gtccggatta cctggaatac
     1741 gccagcctga atttcaccgc aggcctcggc tgtgattttt acgacggcat attccttgcc
     1801 c
//
LOCUS       DAONFI010000398         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_91396_length_1790_cov_3.131412, whole genome shotgun sequence.
ACCESSION   DAONFI010000398 DAONFI010000000
VERSION     DAONFI010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91396_length_1790_cov_3.131412"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PLK08_10175"
     CDS             <1..815
                     /locus_tag="PLK08_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPS53907.1"
                     /translation="YQTASYTKTIVDLIESQKPQICLFGATSIGRDLAPTIASKVRCG
                     LTADCTDLQIGDHEDTKNNVTYSNLLYQIRPAFGGNIIATIVNPKRWPQMATVREGVM
                     PMSDPDAARKGEIVDIKAALGDLILPLTINECHRQAKKVNLKAARIIVAGGAGVGSKE
                     NFKLIWDLAHCLGGAAAGSRAAVDLGFIDHDHQVGQTGTTVRPALYIAVGISGAIQHL
                     AGMQESAKILAINSDPSADIFSVAHYGIVGDLNEVVPLLIKSIRNGMSVKTK"
     gene            995..>1790
                     /locus_tag="PLK08_10180"
     CDS             995..>1790
                     /locus_tag="PLK08_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPS53908.1"
                     /translation="MANFYNDNDDIKFLFKHFDVARLAELMEEGFRFNAEFPHAPENA
                     ADAIDNYQRMLANIGEICGDHIAPTAEETDNVGNVLNEDGSVTYAPGIEKALKLFAQA
                     DAMGFTLPYRFGGINCPQFVFTMSNDIVSRSDAALMNIYGLQGNADTLNAFASEEIKA
                     HYLPKMAAGEWTGAMVLTEPDAGSDLQAVKVRGYQDESGQWYIHGVKRFITNGCGKVL
                     LVLCRTEPEISDGRGLSLLVCENGPQVKIRRLEHKLGINGSPTCEIF"
BASE COUNT          485 a          528 c          452 g          325 t
ORIGIN      
        1 attatcagac cgccagttat acaaagacga tagtggacct catcgaatcg cagaaaccgc
       61 agatttgcct cttcggtgcg acgagcatag gccgcgacct cgccccgacc atcgccagca
      121 aagtccgctg cggccttacc gcagactgca ccgacctgca aatcggcgac cacgaagaca
      181 cgaaaaacaa cgttacgtac tcgaacctgt tataccagat tcgtcccgcg ttcggcggca
      241 acatcatcgc caccatcgtg aatcccaagc gatggccgca aatggcgacc gtccgcgaag
      301 gcgtcatgcc catgagcgac ccggacgcgg cccgcaaggg agaaatcgtc gatatcaaag
      361 cagccctggg cgacctgatt ctgccgttga caatcaatga atgtcaccgc caggccaaga
      421 aggtcaacct caaagccgcc cgcatcatcg tcgccggcgg cgcaggcgtc ggcagcaagg
      481 aaaatttcaa gctcatatgg gatttggctc actgtctcgg cggagcagcc gccggcagcc
      541 gagcagccgt tgaccttggc ttcatagacc acgaccacca ggtcggccag accggtacga
      601 ccgtgcgtcc ggcgctttac attgcagtcg gcatcagcgg tgcgattcag cacctcgcgg
      661 gcatgcagga gtcggcaaag atattggcca tcaactccga cccgtcagcc gacattttca
      721 gcgtggcaca ttacggcata gtaggcgacc ttaacgaagt cgtaccacta ctgattaaat
      781 caatccgtaa cggcatgagc gttaaaacga aataacgaaa attgcaaatt gtaaattaca
      841 gattgctaat tgaaaaatac acagagtgaa tccaaagaaa tattggcaat aacaaccgca
      901 acgcggaaat ctgcaaaaaa aagcagaagc acatgcaagc gttgacaatt tgaaattggc
      961 aatttgaaat taaaaataat ggagaaaata cgatatggcc aacttttaca atgacaacga
     1021 cgacatcaag ttcctgttca agcatttcga tgtcgccagg ctggcggaac tgatggaaga
     1081 aggcttccgc ttcaacgccg aattccctca cgcacctgaa aacgcagccg acgccataga
     1141 caactatcag cgtatgcttg ccaacatcgg cgaaatctgc ggcgaccaca tcgccccgac
     1201 ggctgaagag accgacaacg tcggcaacgt cctcaatgag gacggcagcg taacttacgc
     1261 acccggcatc gaaaaagccc tcaagctttt cgcgcaggca gacgcaatgg gctttacgct
     1321 gccctaccgt ttcggcggca tcaactgccc gcagttcgtc ttcaccatgt ccaacgacat
     1381 cgtcagccgt tccgacgcgg cgttgatgaa catctacggc ctccagggca acgccgatac
     1441 gttaaatgcc ttcgccagcg aagaaatcaa ggcccactac ctgccaaaga tggccgccgg
     1501 cgaatggacc ggcgcaatgg tactcaccga gccggacgcc ggcagtgacc ttcaagccgt
     1561 caaggttcgc ggctatcagg atgaatccgg ccagtggtat atccacggcg taaagcgttt
     1621 catcacgaac ggctgcggta aggtgttgct ggtgctctgt cgcactgagc cggaaatatc
     1681 agacggccgc ggccttagcc ttctcgtctg cgaaaacggc ccacaggtca aaattcgccg
     1741 gctggaacac aagctcggca tcaacggcag tccgacatgc gaaatctttt
//
LOCUS       DAONFI010000399         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_92233_length_1780_cov_5.019710, whole genome shotgun sequence.
ACCESSION   DAONFI010000399 DAONFI010000000
VERSION     DAONFI010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92233_length_1780_cov_5.019710"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLK08_10185"
     CDS             <1..187
                     /locus_tag="PLK08_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53909.1"
                     /translation="VSEFLPQLASTGPWLAAAVFVMCFAVLIVRRFESGAWKKFNLLG
                     PQEDAAVEALSEAPPVV"
     gene            268..804
                     /locus_tag="PLK08_10190"
     CDS             268..804
                     /locus_tag="PLK08_10190"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPS53910.1"
                     /translation="MSRSDAGFYSVFTTAWGPMGAFADGAGAIMGVILPHYSKDDLLA
                     MLAWDCKKAIPNDGPFEKFIAEARRYFNGEVPDFDGMELNMSDAGNFYGKVYRACMAI
                     PFGRTVSYRELAVTIGSEDAARPVATAMSRNPLPLIVPCHRVIHSDGTPGGFSAAGGI
                     DMKLRMINHEKMHLKNSN"
     gene            1233..>1780
                     /locus_tag="PLK08_10195"
     CDS             1233..>1780
                     /locus_tag="PLK08_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPS53911.1"
                     /translation="MMAKNDNQNIYKDTLNLPQTTFDMRANLLEKEPRLQAWWQEQNL
                     YDKICAARKDAEQFILHDGPPYANGNIHMGTALNKILKDIVIRSKNMAGFKVPYIPGW
                     DCHGLPIEAKVMEELGAKARELSPLEIRHKCRAYAEKFVKLHSGQFQRLGVMGEFDNP
                     YITMSPQYEADALEVFARLIEQG"
BASE COUNT          447 a          372 c          478 g          483 t
ORIGIN      
        1 ggtttcggaa tttctgccgc agcttgcgag cacggggccg tggctggctg cggcggtgtt
       61 tgtaatgtgc tttgccgtgt tgattgtgcg acgcttcgag tcgggagcgt ggaagaaatt
      121 caacctgctt gggccgcagg aagatgccgc tgtagaggca ctttcggaag caccgccggt
      181 ggtttaagat attgtcggta gaacggctga gatttttaat ttttcagcca ctgccgctgc
      241 gttcggaatg aaaaggaatt ttggaaaatg agcagaagtg atgcaggatt ttacagtgtg
      301 tttactacgg catgggggcc tatgggcgcg tttgctgacg gggccggcgc gattatgggc
      361 gttattctgc cgcattattc caaagatgat ttgctggcga tgttggcgtg ggattgcaaa
      421 aaagcgattc cgaacgacgg gccttttgaa aagtttatcg ctgaggcgcg acgatatttt
      481 aacggcgaag tgccggattt cgacggcatg gagttgaaca tgtcggatgc cgggaatttt
      541 tacggcaagg tttatcgtgc ctgcatggcc atacctttcg gccggacggt gagttatcgt
      601 gaactggcgg tgacgattgg cagcgaggac gcagcacggc ctgtcgccac tgccatgagc
      661 cgtaaccctc tgccgcttat tgtaccctgt catcgcgtga ttcactccga cggtacgcca
      721 gggggatttt ccgccgccgg cggaatcgac atgaaattgc ggatgataaa tcacgaaaaa
      781 atgcatctta aaaattcaaa ctgaccgatt taacggtttt cagtatatta tgcgtaaagg
      841 taaatactta ttttacatat tgttatgatt cgactgcaat cgtttgtcaa tctattttgt
      901 caaattgaca atttatgtaa ctttttttaa atgagtattt accgaaccaa atatcgtcaa
      961 tcggtcactt tgagttaaaa aaaatggaag cgtttataaa attccgacgg gcttttgaga
     1021 gccgttccaa tcggtttgtt tttcagcctt tgccgcgtaa aatgacggaa tatcgataaa
     1081 aaatgtcttg ctcttttatt ttacggctga cggtaaaaaa tctttcagct gagccgtgaa
     1141 agcagttttt gaatgacttt taaatgtttt gtttactttt tctgcaaaaa acggtatatt
     1201 tcctgcctaa ttttggtgac ttttcggagt agatgatggc aaagaatgac aatcagaata
     1261 tttacaagga tacgctgaat cttccgcaga cgacgttcga tatgcgggcg aatctgttgg
     1321 aaaaagagcc tcgcctccag gcttggtggc aggaacagaa tctttacgac aaaatctgtg
     1381 cagcccgcaa ggacgccgag cagttcattc tgcacgatgg cccgccgtac gccaatggca
     1441 atatacacat gggcacagcc ctgaacaaga ttttaaaaga catagtaatc cgcagcaaga
     1501 atatggctgg ttttaaagtg ccctatattc ccggctggga ttgtcacggg ttgcctatcg
     1561 aggctaaggt catggaggag ctcggagcta aagcccgcga gctttcgccg ctggaaatcc
     1621 gtcacaaatg ccgcgcttat gcggaaaaat tcgtcaaact tcatagcggg cagtttcagc
     1681 gtctgggcgt tatgggcgag ttcgacaacc cgtacataac catgagtccg caatatgagg
     1741 cggacgcgct ggaagtgttc gcgagattaa tcgaacaggg
//
LOCUS       DAONFI010000400         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_96994_length_1727_cov_4.604067, whole genome shotgun sequence.
ACCESSION   DAONFI010000400 DAONFI010000000
VERSION     DAONFI010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96994_length_1727_cov_4.604067"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..837)
                     /locus_tag="PLK08_10200"
     CDS             complement(133..837)
                     /locus_tag="PLK08_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPS53912.1"
                     /translation="MRTKKAFSLIELLMVVGIISLLASILLPSLSQAKEMSRKILCQV
                     NLGNIYKAIEMYRSNYNDIYPARQDPVSTSPFYWTWMGRGMRDVMADELAMHAGISGA
                     HSVMLCPADPAAKTTYDATSYAYSMSFYHSLKQINDMVDKVYSYTCQSEYPTIVQNGN
                     ALTYTSKKILSGEWNGNHDDFASDNGWWSDYGARNFVFADGSVRFLRAGEMHTANDGY
                     PDPNLTTDGISGQDVD"
     gene            complement(891..>1727)
                     /locus_tag="PLK08_10205"
     CDS             complement(891..>1727)
                     /locus_tag="PLK08_10205"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPS53913.1"
                     /translation="VLGLGVFDVAASAALENANVKATDLDLIIVATCTPDMPLPSTAC
                     FVQKAIGATEVPCFDLAAACSGFIYGLTVGSQFIETGKYKKILVIGADSLTTMTDYQD
                     RGSCIIFGDGAGAVILEPTADENVGVLYTVLKADGEGWDFICTPAGGSKTPASHETVD
                     ARDHFIKMKGREVYKFAVEKMQWLLGDCMEKTNLTVNDVDLVVPHQVNIRIIESATEK
                     HKFPIEKVFVNIDKRGNTSAASIPLALDEAARHGLLSSGKTIVMVAFGAGLTWAGAVI
                     KF"
BASE COUNT          432 a          434 c          412 g          449 t
ORIGIN      
        1 ctcgtcctca acgacgcaac aaggcgcaat ttcaaattta acatgctcta aaaaatccaa
       61 gtggtatttt tctgaacgat aatataacgt tttttaaaaa ttactttcct cttaaaaaaa
      121 tattttaacc tgtcaatcaa catcctgccc tgaaataccg tcggtagtaa gattcggatc
      181 agggtaaccg tcgttcgcgg tgtgcatttc cccggcacgc aaaaaccgga ccgacccgtc
      241 agcgaaaaca aaattccttg cgccgtaatc gctccaccag ccgttatccg atgcaaaatc
      301 gtcatggttg ccgttccatt ctcccgaaag tatctttttt gacgtatatg tcagtgcatt
      361 gccgttctgc acaatggtgg gatattccga ttgacacgta taggagtata ccttgtcgac
      421 catatcgttt atctgtttta aagaatgata aaaactcatg gaataggcgt aactcgtagc
      481 gtcgtatgtc gtcttggcgg ctgggtcagc cggacagagc atgacggaat gcgcaccgga
      541 tataccggca tgcatggcaa gctcatcagc catcacgtcg cgcatgccgc ggcccatcca
      601 cgtccagtaa aacggcgacg tcgaaacagg atcctgcctg gccgggtaaa tatcattata
      661 attactgcga tacatctcga ttgctttata aatattgccc aaattgacct ggcagaggat
      721 tttgcggctc atctccttag cctgcgaaag cgacggcaga agaatgctgg ctaataatga
      781 tattatcccc acgaccatca gaagttcgat tagtgaaaag gctttttttg tgcgcatatt
      841 ccgactcccg taataatgga ttccgagatt ttccgtaaca ggcgtgcgtt tcagaatttt
      901 ataactgcgc cggcccaggt aaggcctgcc ccgaaagcaa ccatgacaat ggttttgccg
      961 gaactcagga gaccgtgcct ggcggcttcg tccaatgcaa gcggtatcga tgccgccgac
     1021 gtgttgccgc gtttgtcgat gttgacgaaa actttttcta tcggaaactt gtgcttttcc
     1081 gttgccgact cgattatgcg aatgtttacc tgatgcggca cgacaagatc aacatcgttt
     1141 actgtcagat tggttttttc catgcagtcg ccgagcagcc attgcatttt ttctacggca
     1201 aacttgtaga cttcgcggcc tttcatcttt atgaaatggt cgcgggcatc cacggtttca
     1261 tgtgacgcag gagttttcga cccgccggct ggcgtgcaga tgaaatccca gccttcgccg
     1321 tcggctttca ggactgtata aagaacgccg acattttcgt cggcagtagg ttcaagaatc
     1381 accgctccgg cgccgtcacc gaatatgata caactgcccc tgtcctgata atcggtcatg
     1441 gtcgtaagcg agtcggctcc gataacgagg atttttttat atttcccggt ttcgataaac
     1501 tggctgccta cagtcaaacc gtaaatgaaa ccactgcagg ctgccgccag gtcaaaacat
     1561 ggtacttcgg tagcaccgat ggctttctgg acaaagcagg cggtcgaagg aagcggcata
     1621 tcgggagtgc aggtggctac gataatcagg tcaaggtcgg ttgcttttac gttggcgttt
     1681 tcaagcgcgg cagaggcagc tacgtcaaag accccaaggc cgagaac
//
LOCUS       DAONFI010000401         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_97698_length_1720_cov_3.664865, whole genome shotgun sequence.
ACCESSION   DAONFI010000401 DAONFI010000000
VERSION     DAONFI010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97698_length_1720_cov_3.664865"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="mutL"
                     /locus_tag="PLK08_10210"
     CDS             complement(<1..1028)
                     /gene="mutL"
                     /locus_tag="PLK08_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPS53914.1"
                     /translation="MPKISQLSQHLVNKIAAGEVIERPASVIKELVENAIDAGATRID
                     IAIEDGGKKLIQVTDDGGGMERDDAALAFAPHATSKLRDEDGLFNICTMGFRGEALAS
                     IASISHAHIRTRRRKDANGWEISASGENITAVTPCPAAAGTTITVRDLFFNTPARRKF
                     LKTASTEFGHITEQVARLSLPHPQISFTVRHNGRETVNLPACQSTLQRITDLFTPDTA
                     ENLIPIPQRNAITTINGLIGTPAAARNSTKWQYVFLNGRYIRDRLISHAIKEAYRGRI
                     MPAKCPVAFIFINIDPHEVDVNVHPTKIEVRFRNSNGVYGDVLAALKETLSHSNLAPD
                     MQTQNFFD"
     gene            complement(1028..>1720)
                     /locus_tag="PLK08_10215"
     CDS             complement(1028..>1720)
                     /locus_tag="PLK08_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53915.1"
                     /translation="KTTKDYFKLKRLLSLTDQLQPNIKNLWAKPEIGADSLKISFKNK
                     PSICTTFISSYCMVTFAMIFIMAFKYGFEMSLEQTLTWIKIALSISVVGVILSIIKYI
                     FSRPEVLTIDRVTGEIVFESKKENWRLSLSDIKNLRLKIDVSSFQRNNKQVKIYRLEA
                     IDKSNMWHTIHQRKISLNSDWLLLNKSMLAGRTLAAFIGCPMDDQVEINDRNKKIPPF
                     PKKFIKIPDKIN"
BASE COUNT          359 a          376 c          453 g          532 t
ORIGIN      
        1 ctgtcgaaga aattttgtgt ttgcatgtca ggcgcgagat ttgagtggct gagcgtttcc
       61 tttaacgcag ccagtacgtc gccgtatacg ccgttggagt tgcggaatcg cacttcgatt
      121 tttgtcggat ggacgtttac atccacttcg tgagggtcga tattgatgaa tatgaacgcc
      181 acggggcact tggccggcat aattctgccg cggtaagctt cttttattgc gtggcttatc
      241 agcctgtcgc gaatatatcg cccattcagg aatacgtact gccatttggt actgttgcgt
      301 gcagccgccg gcgtgccgat taacccgttg atggtagtta tggcgtttcg ttgtgggatg
      361 gggatgaggt tttctgccgt gtcgggcgtg aagaggtctg ttatgcgttg cagggtggac
      421 tggcaggctg gcagatttac ggtttcacgt ccgttatgac gaacggtgaa tgatatttgc
      481 ggatgcggca gcgacagccg cgcaacctgc tcggttatat ggccgaattc ggtggaggcg
      541 gttttcagaa atttacggcg tgccggcgta ttgaagaaca ggtcacgcac cgttattgtc
      601 gtgccagccg ccgccggaca tggcgtgacg gcggttatat tctcaccgct ggctgaaatc
      661 tcccagccgt ttgcgtcttt gcgccgtcgc gtgcggatgt gagcgtgcga aatgctcgct
      721 atgctggcta gcgcctcgcc gcgaaaaccc atcgtacaga tgttgaacag gccgtcctcg
      781 tcacgcagtt tgctggtggc gtgcggggcg aaggcaagag cggcgtcgtc tcgttccatg
      841 ccgccgccgt cgtcggttac ctggatgagc tttttgccgc cgtcctctat ggcgatgtcg
      901 atgcgcgtag caccggcgtc gattgcgttt tcgacgagtt ccttgatgac gctagccggc
      961 cgttctatga cttcgccggc tgctatctta tttacaaggt gctgcgatag ttgggatatc
     1021 tttggcatca gttaatctta tcaggaattt ttataaattt ctttggaaag ggaggtattt
     1081 ttttatttct gtcatttatt tcaacttggt cgtccattgg gcaaccgata aatgccgcca
     1141 gtgttctgcc ggcaagcatg gatttattca gtaacaacca atcactgttt aaggaaattt
     1201 tgcgctgatg tattgtatgc cacatattgg atttgtctat tgcttccaac cgatagattt
     1261 ttacttgttt attgtttctt tgaaaggacg atacatcaat ttttaaccgc agatttttaa
     1321 tatcgctcag tgacagccgc cagttttcct ttttcgactc gaatactatt tcacccgtga
     1381 ctctgtctat ggttaaaact tcgggccggc tgaaaatata ttttataatc gaaagtatta
     1441 ccccaacaac gcttatggag agagcgattt ttatccacgt taatgtctgt tccagagaca
     1501 tttcaaagcc gtatttgaag gccattatga atatcattgc aaaagttacc atgcagtagc
     1561 tgcttatgaa tgtcgtgcat attgacggtt tgtttttaaa agaaatcttg agcgaatcag
     1621 ctccgatttc aggtttggcc cagaggtttt tgatatttgg ctggagctga tcagtaagac
     1681 tcagtagtcg tttcaattta aaatagtctt ttgtcgtttt
//
LOCUS       DAONFI010000402         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_98076_length_1716_cov_4.460566, whole genome shotgun sequence.
ACCESSION   DAONFI010000402 DAONFI010000000
VERSION     DAONFI010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98076_length_1716_cov_4.460566"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /gene="pilO"
                     /locus_tag="PLK08_10220"
     CDS             <1..500
                     /gene="pilO"
                     /locus_tag="PLK08_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPS53916.1"
                     /translation="GNRAVLAALGHVDAEAASVQGARAGESQRHLHHEDLASLMSLKD
                     KAAARLPQSEDMGPVIDELSKIANVNKLKISKMQNVALSDKEKLELADTYYGIQRLDV
                     ELEGNYVGFYKFLTDMEKQPRIIRIQNLDIQRINEGDRQGDVKVRMDLRVYYGKAKAD
                     QAVKK"
     gene            497..1135
                     /locus_tag="PLK08_10225"
     CDS             497..1135
                     /locus_tag="PLK08_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53917.1"
                     /translation="MSEPANNLSEYQGPGPVSRTESVELPPIVDAGKKHDIKYIRSTL
                     VMLLMFCAGLGWVYFEGRQGIKAAGQDARLVTVKSALAAMRPNALSGEDRKLSREKLL
                     SPLEFMPTQCQIPATQLIFNPFEIYLYRQAVQQEASKPKESEKTKVVEEKAPSVKKMV
                     LESVLLGSRPAAMINGKTVYIGQKVGGWTLQEIKYNRVILRWKNRTHVLKME"
     gene            1138..>1716
                     /locus_tag="PLK08_10230"
     CDS             1138..>1716
                     /locus_tag="PLK08_10230"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53918.1"
                     /translation="MEDNNKESKSDKIYGLENLWSPDESANRSVGPERILLEKGRITP
                     GQLEQARQRQQDDPRLSLMEALVRINAVSSEETLEAIAEYFRLPFRRVNYADVQKKAF
                     NVLPVEYLRAKKVLPIEFYNDGIVIAISDPADIFLVDDFRRRLNMKVELVVTPVTDIR
                     TAIDELSAGPGQKVDDIVADFDDSSVEVVEESE"
BASE COUNT          480 a          434 c          461 g          341 t
ORIGIN      
        1 acggcaatcg tgcggtgctg gcggccctcg gtcacgtcga cgccgaggcc gcgagcgtgc
       61 agggtgcgcg tgccggcgag tcgcagcggc atctgcatca cgaagacctc gcctcgctga
      121 tgtcgctcaa ggataaagcc gccgcccgtc tgccgcagtc cgaagacatg ggccccgtaa
      181 tcgacgaatt gtccaaaatc gccaatgtaa acaaactcaa aataagcaaa atgcagaacg
      241 tcgctctttc cgataaggaa aaactggaac ttgcggatac atattacggc attcagcggc
      301 tggatgtgga gctggaaggc aattatgttg gtttttacaa atttttgacg gatatggaaa
      361 aacagcccag aatcatccgc atacagaatc tggacattca gcgcatcaat gaaggcgacc
      421 gccagggtga cgtaaaagtg cgaatggatt tgcgtgtcta ttacggcaag gctaaagcag
      481 accaggcggt gaaaaaatga gcgaacctgc aaataacttg tcggaatacc agggtcccgg
      541 gcctgtctcc agaacggaat cagtagaact gccgccgata gtggacgccg gcaaaaagca
      601 tgacataaaa tacatacgca gtacgctggt aatgttgctg atgttctgtg ccggccttgg
      661 ctgggtctat tttgaaggcc gtcagggcat caaagccgcc ggccaggatg cacggctggt
      721 cacggtaaaa tctgctctgg ctgcaatgcg gcccaacgct ctgagcggtg aagacagaaa
      781 gttatccaga gaaaaactcc tctcgccgct ggaattcatg cccacgcaat gccagatacc
      841 tgctacccaa ctgatattca atccgtttga aatatatctt taccgccagg ctgtccagca
      901 ggaggcgtca aagcccaaag aatccgaaaa aaccaaagtg gtcgaggaaa aagccccgtc
      961 cgtcaaaaaa atggtactgg aatcggtgct tctcggcagt cggccggcgg cgatgataaa
     1021 cggaaaaacc gtatatatcg gccagaaagt aggcggctgg acgttgcagg aaatcaaata
     1081 caaccgcgtc attcttcgct ggaaaaacag aacgcacgtc ctgaaaatgg aatgagaatg
     1141 gaagataata ataaagaatc aaaatccgat aagatatacg ggctggaaaa tttgtggtcg
     1201 ccggacgaat ccgccaacag gtctgtcggg ccggaacgga ttttacttga aaagggccgc
     1261 ataactcccg gccagcttga acaggccagg cagcgacaac aggatgaccc gcggctgagc
     1321 ctgatggaag cgcttgtcag aataaacgcc gtctcctccg aagaaacgct cgaagccatc
     1381 gccgaatatt tccgtctgcc gtttcgtcgc gtgaattacg ccgacgttca gaaaaaagcg
     1441 ttcaacgttc tgcctgtgga atatctccgc gcaaaaaaag tccttccaat cgaattctat
     1501 aacgacggca tagtgatagc gatttccgac cctgccgata tttttcttgt ggacgatttc
     1561 cgccggcggc ttaacatgaa ggttgaactt gtcgttacgc cggtcactga cattcggacc
     1621 gctatcgacg aactttcagc cgggccgggt cagaaagtcg acgacatagt ggctgacttc
     1681 gacgacagca gcgtcgaggt cgtcgaggaa agcgaa
//
LOCUS       DAONFI010000403         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_99395_length_1702_cov_3.041894, whole genome shotgun sequence.
ACCESSION   DAONFI010000403 DAONFI010000000
VERSION     DAONFI010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99395_length_1702_cov_3.041894"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLK08_10235"
     CDS             <1..536
                     /locus_tag="PLK08_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPS53919.1"
                     /translation="AQAQKEACDTDTAAETMRRILSSRTPAECQPWMYMFLGDCEMQN
                     GNPEKAFSAYRTACKLDRQDISTWVSMAQACIVMKKYYTAANATVEALRIKPDDLQAN
                     LVYAYSLMKLNRAKQAAERLEQLTRLYPQNGTLCYLLARAYQMQGDNERARRLLGVAM
                     QGNPESQLARQMLAKGN"
     gene            complement(892..1056)
                     /locus_tag="PLK08_10240"
     CDS             complement(892..1056)
                     /locus_tag="PLK08_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53920.1"
                     /translation="MKSLTLRLTLASTMPAILPETINYDNYDSGTTTIRWFVPQLAMT
                     DILPCERPLF"
     gene            1081..1446
                     /locus_tag="PLK08_10245"
     CDS             1081..1446
                     /locus_tag="PLK08_10245"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53921.1"
                     /translation="MKKAFTLIEILIVVILLGILAAVVIPQFVDQTAVVNKNAAATEV
                     ANLNSAWQQYVAAGMADSDDATPINAATFTAACQALLDGHYIDTLPTSTKYEGWTLEF
                     TQGATPQQSKFAATEVAAG"
BASE COUNT          453 a          381 c          443 g          425 t
ORIGIN      
        1 tcgcccaggc acagaaagaa gcctgcgaca ccgacaccgc cgctgaaacc atgcgccgta
       61 ttctgagttc ccgcacgccg gccgaatgtc agccgtggat gtatatgttc ctcggcgatt
      121 gcgagatgca gaacggcaat ccggaaaaag ccttttccgc atatcgcacc gcatgtaagc
      181 ttgaccggca ggatatttcg acatgggtga gcatggcaca ggcatgtata gtcatgaaga
      241 aatattatac cgccgccaat gccaccgtcg aggcattgcg aataaagccc gatgatttgc
      301 aggctaatct ggtttacgcg tattcgctta tgaagctgaa tcgtgcgaag caggcagctg
      361 agcgtctgga gcagctgacg cggctgtatc ctcagaatgg aacgctctgc taccttctcg
      421 cccgtgccta tcagatgcag ggcgacaatg agcgggctcg ccgtttgctc ggcgtggcga
      481 tgcagggcaa tcctgaaagt caattagcca ggcaaatgct ggcaaaagga aattgatgtt
      541 gaatttccaa attcgaagct gatgcaacca agtgtccaat tagcgtagat atgagcaatt
      601 atgaggtttt tagcgggttg atttgcgaat tttcaaaaaa aaatgttcag tcattggttt
      661 tattggaata attgtgtata gaataattat gcggcgatga ggccgctaag gcttttgaga
      721 tttacaattt acgattttca aaggtcagta ttatcgcgac tcctggagcg tgcgggacaa
      781 aatagattaa tatccgccgc tcgcgagcga tggcccttgg gcatggcagt ctgatgcgat
      841 acccgtttga acgattgttt caatagaaaa taaaaagtcc ggaaaggagc atcaaaaaag
      901 aggacgttca cacggaagaa tgtcggtcat ggccaactga ggaacaaacc atcgtatcgt
      961 agtcgtgccg ctatcataat tgtcataatt tattgtttcc ggaagaatcg ccggcatggt
     1021 gctggcgagt gtcagccgga gggttaatga tttcaaaaaa aattttgaaa ggatcgagaa
     1081 atgaaaaagg catttacact tattgaaatt ctgatcgtgg ttatccttct gggtatcctc
     1141 gcagcggttg tcattccgca gttcgtagac cagacggcag tcgtcaataa gaatgcggca
     1201 gcgacagaag ttgcaaatct gaattcagca tggcagcagt atgtagctgc tggcatggct
     1261 gatagcgatg atgcaacgcc aattaatgct gcaacgttta cggctgcatg ccaggcactg
     1321 cttgatggcc actatattga cactctgccg acctcgacta agtatgaagg ttggacactt
     1381 gagtttaccc aaggtgcaac gccgcagcag agcaagtttg ctgcaacaga agttgctgct
     1441 ggctaaccac tgattcagac atgaactgaa aaatcccgac gggttttggc ccgccgggat
     1501 ttttttatgt tttataattt cctcacataa aatcccagcc ttgccagggt atcttacgtg
     1561 tgcggttttc aaaactgcca gttgagcatt acgctccatg tgccgtcggc atcggcattg
     1621 gcgaaattgc cgaacagtcc gtacgcaccc tgcagagtga gattgtcggt tatgatgata
     1681 tctgccgtga tagcctgcca gt
//
LOCUS       DAONFI010000404         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_101713_length_1678_cov_3.802834, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000404 DAONFI010000000
VERSION     DAONFI010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101713_length_1678_cov_3.802834"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..348
                     /gene="rpmF"
                     /locus_tag="PLK08_10250"
     CDS             166..348
                     /gene="rpmF"
                     /locus_tag="PLK08_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPS53922.1"
                     /translation="MVPKYKISRSRTRRRRSHNAHVPANYVDCPRCNSPKLPHAACDN
                     CGYVRPGLSLKVNEEK"
     gene            395..1378
                     /gene="plsX"
                     /locus_tag="PLK08_10255"
     CDS             395..1378
                     /gene="plsX"
                     /locus_tag="PLK08_10255"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPS53923.1"
                     /translation="MGGDRGISVVVAGAVAAQSRLDSGDRIVLFGDRDAITAELAKAD
                     DVSLIEVVHTSQVVAMDDSPVEAIRSKKDSSLVVMTVQHREGEIDACISAGNTGACVA
                     AAQMYMRRLKGVARPGIMVVAPTFKGIFALCDVGANVNCRPQNLLQYGIMCSEYLKYT
                     HNVENPRVGLISIGEEDVKGNDLVKQTNELMRAEKRIRFVGNVEGRDILRGVADVMVC
                     EGFVGNVILKVIEGMAESIVKGMLQKFEEIMPDQLGRVLQVVGKLAEEFDSNNYGGAP
                     LLGMDGIWLICHGASSSSGIMNAILKAKIFGSQDVNQHILEHLANDVEVTV"
     gene            1543..>1678
                     /locus_tag="PLK08_10260"
     CDS             1543..>1678
                     /locus_tag="PLK08_10260"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547226.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HPS53924.1"
                     /translation="MAGVRAKITGFGSAVPSRVMTNKDFEKIVDTTDEWIVQRTGIRQ
                     R"
BASE COUNT          411 a          388 c          498 g          381 t
ORIGIN      
        1 ggtttttttt cttgacaaag aggcgtttag cgggtatttt tcccgctccg tacgtaaaac
       61 gggcgcgcag gtgttgtgcg caaaatgatt tttgcaaggt tatatgcggg ctggcattgc
      121 gctgcacagc ccattttggt tttacaggat acaggaacag gaaaaatggt accgaaatat
      181 aaaatcagcc gttcacggac acgtcgccgt cgtagccata atgctcacgt accggctaac
      241 tatgtagatt gcccccgttg caattctccc aagctgcccc atgcggcgtg tgataattgc
      301 ggctatgtcc ggcccgggct gagcctcaag gtcaacgaag aaaagtaagc cagaaactat
      361 ctggagcgag tatatgcgta ttggtgtcga tgctatgggt ggtgatcgcg gcatatcagt
      421 cgtggtcgcc ggcgcggtag ccgcccagtc caggttggac tcaggcgacc ggattgtcct
      481 gtttggcgac cgtgatgcta ttaccgccga attggccaag gctgacgatg tctcgctgat
      541 agaagtagtc catacgtcgc aggtcgtggc gatggacgat tcgccggttg aggcaatccg
      601 ttcgaaaaag gattcctcgc tggtggttat gactgttcag catcgtgaag gcgaaattga
      661 cgcctgcata tcggcgggca ataccggcgc atgcgtggcg gctgcccaga tgtatatgag
      721 acgtctgaag ggcgttgcca ggccgggcat aatggtggtg gcaccgacgt ttaagggcat
      781 attcgccctg tgcgacgtcg gggctaacgt aaactgtcgg ccgcagaatc tgctgcaata
      841 cggcatcatg tgctcagagt atctcaagta tacgcacaat gttgaaaatc cccgcgtcgg
      901 tcttataagc atcggcgagg aggacgtcaa aggcaacgac cttgtaaagc agactaatga
      961 attgatgcgg gcagaaaagc gcatccggtt cgtaggcaac gtcgaagggc gggacatcct
     1021 gcgcggcgta gccgacgtga tggtctgcga agggtttgtg ggcaatgtca tcctcaaggt
     1081 tatcgagggg atggcggaaa gcatagtcaa gggcatgttg cagaagtttg aggaaatcat
     1141 gcccgaccag ctcggccgcg ttttacaggt cgtcggcaag cttgcggaag agtttgattc
     1201 gaacaattac ggcggtgcgc cgcttctggg catggacggc atatggctga tatgccacgg
     1261 tgccagcagt tccagcggca taatgaacgc catattaaaa gccaaaattt tcggcagtca
     1321 ggatgttaac cagcatattc tggaacatct ggcgaatgat gttgaagtaa cggtctgaaa
     1381 acagccgccg cttgaatttt ttattgcaga atgaatcttt acttttgcga aaaatgcacc
     1441 atgagccggc cagccggaat cggctgactg gaaaagtagg aacaaaaaaa ttctggcggg
     1501 gcccccataa tggggccttt tacttaatga ttggagcaat caatggcagg tgtcagagca
     1561 aagataaccg gcttcgggtc ggctgtcccg tccagggtga tgaccaacaa ggattttgag
     1621 aagatagtcg acaccaccga cgaatggata gttcagcgca ccggcatacg tcaacggc
//
LOCUS       DAONFI010000405         1668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_102658_length_1668_cov_4.122753, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000405 DAONFI010000000
VERSION     DAONFI010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102658_length_1668_cov_4.122753"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..676)
                     /locus_tag="PLK08_10265"
     CDS             complement(71..676)
                     /locus_tag="PLK08_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53925.1"
                     /translation="MAIYCEKRGFTLIELLVVISIISLLTVVLLPSMNKARQISKRTA
                     CRALLSGVGRAIDIYKGQYNSLPWARELSTITSSQTDLLYLENVPISKALEEFADEKQ
                     FKCPADKGPIGDTDESKEERASFGNYESYFERERTSYDYFQLFQMESTAPEIYKNMLS
                     HGDSKILVMNDFEPFHGERLKSGSRNGLYGDGHVSDTVTGD"
     gene            complement(789..>1668)
                     /locus_tag="PLK08_10270"
     CDS             complement(789..>1668)
                     /locus_tag="PLK08_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53926.1"
                     /translation="DDVITLPGRKIKLAAHCDIEDAQNEALVVTYSLDGKELGRVNVA
                     AEESTVIINWTPPAAGIYNIAVTANYKEKKTFLPKMRAVNLIVCVAAADSPMMIVDLD
                     HTVVGSGFFKVVMRMAQPMPGAAAVLGKFSQNYTIVYLTHRPAMLTSLSKKWLMANNF
                     PMAPLLANQDNRFSSEEYKSCRIKELRQDFGNIQVGIGDKDSDMTAYRNNNMRAFWII
                     KYKDKRKTIEKIIKKIQPFRRDKNVQVVFAWEQIEQAMNGQRTFPVELVEKQLKIRAE
                     ELKKDKKHEDDDDDDE"
BASE COUNT          395 a          430 c          348 g          495 t
ORIGIN      
        1 tgccattttt cttccttgcc agcccgcaaa attgctcgat ttcggcgacg cccatatttt
       61 tggataggtt ctaatcaccg gttaccgtat cagagacgtg gccatcgccg taaaggccat
      121 tccgagaacc gctcttaagc cgctcgccat ggaaaggctc aaaatcattc atgaccagaa
      181 ttttcgaatc gccgtgcgac aacatatttt tataaatttc aggcgcagtg gactccatct
      241 gaaacagctg gaaataatcg taactggttc gctcccgctc aaaatacgat tcgtaatttc
      301 caaaagacgc ccgttcctcc ttggattcat ccgtatcgcc tatcggtccc ttgtcagccg
      361 ggcatttgaa ctgcttttca tcggcgaatt cttccagtgc ctttgaaatc ggcacatttt
      421 caagatacag aagatccgtc tgggacgaag ttatggttga caactcacgc gcccagggca
      481 gggagttgta ctggccttta tatatgtcga tagccctgcc gacgccgctc agcagtgcgc
      541 ggcaggcagt acgtttgctt atctggcgag ccttgttcat cgacggaagc aaaacgaccg
      601 tcaacagaga gatgatggat attaccacca gtaactctat taacgtaaat cctctttttt
      661 cgcagtatat ggccatatag tctcagttaa actaacgcac tgacggctga tttatttcaa
      721 tcactaaaaa catgaaaaat cacacactta tttatgtatt ataccatttt ccagcattct
      781 cagataggtc actcgtcgtc atcgtcatcg tcttcatgtt ttttgtcttt tttaagctct
      841 tcggcacgaa ttttcagctg tttttctacc agttccaccg gaaatgtcct ctggccattc
      901 atcgcctgtt ctatctgttc ccacgcgaaa acgacctgca cgtttttgtc acggcggaaa
      961 ggctgtattt ttttgatgat tttttcaatg gttttacgtt tgtctttgta tttgattatc
     1021 cagaatgcgc gcatattgtt gttgcgataa gccgtcatgt ccgaatcctt atcgccgatg
     1081 cctacctgga tattcccgaa atcctggcgt aattctttta ttcgacagct cttatattct
     1141 tcgctgctga acctgttgtc ctgattggcc agcaatggcg ccatgggaaa attattggcc
     1201 ataagccatt ttttcgagag actcgtcaac atcgccggcc tatgggtaag ataaactatc
     1261 gtataattct gtgaaaattt ccccaaaact gccgccgcgc ctggcatcgg ctgagccatt
     1321 cgcatgacaa ccttgaaaaa tccgctgccg acgacggtat ggtcaaggtc gacaatcatc
     1381 atcggcgaat ccgccgccgc aacgcatact ataagattga ccgcccgcat tttgggcaaa
     1441 aatgtctttt tttccttata attcgcagtg accgcgatat tgtaaattcc cgcggccggc
     1501 ggagtccagt ttatgattac cgtactttct tcagccgcaa cattgactcg gccaagttct
     1561 ttgccgtcaa ggctgtacgt gacgaccaaa gcctcgtttt gtgcgtcttc aatatcgcaa
     1621 tgcgccgcca atttgatttt tcggccgggc agcgtgataa catcgtct
//
LOCUS       DAONFI010000406         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_102867_length_1666_cov_4.833644, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000406 DAONFI010000000
VERSION     DAONFI010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102867_length_1666_cov_4.833644"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLK08_10275"
     CDS             <1..351
                     /locus_tag="PLK08_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53927.1"
                     /translation="PVVFAIICGFIMSIFRFNGCDGDCGQCEFRKEGETHSHDMPTEQ
                     CRGGLEGWKLAGSAALVFLFPILTALAGVLLYGHDADSQLGGGIAGAAAGLLVAMFII
                     KVLFRKRNRKNQEK"
     gene            357..>1666
                     /gene="rsxC"
                     /locus_tag="PLK08_10280"
     CDS             357..>1666
                     /gene="rsxC"
                     /locus_tag="PLK08_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPS53928.1"
                     /translation="MTTSTTAMGTFRNGVHPPERKGLAADSIIEPLPTPVEVKVPMLQ
                     HVGAPCELKVKLKQELSWGEIIGSSESFVSSPVHATVSGVAGMACSVTLPNGRRVQAL
                     PITVNDKMPSPEDVLKNVLGGEWPLAGLEQYDSEDIIAAIKAAGIVGQGGAAFPTFIK
                     LIRNEKKPIDTVLVNGCECEPYLTADYRLMVEVPDAILCGAILAARACGAGRIVVAVE
                     DNKPLAVDILRRHAAGTDVEIAELLTKYPQGGEKQTVYAALGREIPTGGLPLDVGVGV
                     INVGTACAIARAVTRGKPLTHRVVTVSGEGVVRPRNILVPIGTKIRRLIEFCGGVKQN
                     AAKIIAGGPMMGFTIGDLDTPVTKGTSGITVLTQDDVDTAQPTNCVRCGRCVDACPMR
                     LVPQRLALGSRNRNWDVVKKYNILSCVECGCCAYVCPAGIELVQL"
BASE COUNT          382 a          442 c          499 g          343 t
ORIGIN      
        1 cctgttgttt ttgctattat ctgcggtttc attatgagca tcttcaggtt taacggctgt
       61 gacggagact gcggtcaatg tgaatttcgc aaggaaggcg agactcacag tcatgacatg
      121 cccacagagc aatgccgcgg cggccttgag ggctggaaac ttgccggcag tgctgcactg
      181 gtgtttttat tcccgatact tacggctctt gcgggagtgt tgctatacgg ccatgacgcg
      241 gacagccagc ttggcggcgg aattgccgga gctgcggcag gcctgcttgt tgcgatgttc
      301 ataatcaagg tgttattcag aaaacgtaat agaaaaaatc aggagaaata actgaaatga
      361 cgacatcgac tacagctatg ggtacatttc gtaacggcgt acatccgccg gaacgcaaag
      421 gcttagctgc cgacagcata atcgaaccgc tgccgacgcc ggttgaagtc aaagtaccca
      481 tgcttcagca cgtcggcgcg ccttgcgagc tgaaggtcaa gctcaagcag gaactttcct
      541 ggggtgagat aatcggctcg agtgaatcgt tcgtgtcgtc gcccgtgcat gcgacggtaa
      601 gcggcgttgc cggcatggcc tgttccgtga cgctccccaa tggccgacgc gtccaggcgc
      661 tgccaatcac agttaacgac aaaatgccat cgcctgaaga tgttttaaaa aatgtcctcg
      721 gcggagaatg gccgctggcc ggcctggaac agtacgattc ggaagatatc atcgccgcta
      781 taaaagccgc cggcatagtc ggccagggcg gcgcagcctt tccgacgttc atcaaactca
      841 tccgcaatga aaaaaaacca atagataccg tgctggtcaa cggctgcgag tgtgagccgt
      901 atcttaccgc agattacagg ctgatggtcg aagtgcccga cgcgattctc tgcggggcta
      961 ttctcgcagc ccgtgcgtgc ggcgcaggca gaatcgtcgt cgcggtggaa gacaataaac
     1021 cgctggctgt cgatattctc cgacgtcatg ccgctggcac ggatgttgag atagcggaac
     1081 tgttgaccaa atatccgcaa ggcggcgaaa agcagaccgt ctacgccgca cttggccgtg
     1141 aaatccccac aggcggtttg ccgctggatg tcggggtggg cgtaataaac gtcggcactg
     1201 cctgcgcaat cgccagagcc gtcactcgcg gcaagccgct tacgcatcgc gtcgtgacgg
     1261 tcagcggcga aggtgtagtt cggccgcgaa acatcctcgt gccgataggc acgaaaattc
     1321 gacggcttat agagttctgc ggcggcgtga agcagaacgc agccaaaatc atagccggcg
     1381 gcccgatgat gggctttaca atcggcgacc tcgacacgcc ggtgactaag ggtacgagcg
     1441 gcataacagt tctgacgcag gacgatgtcg ataccgctca gccgacgaat tgcgtgcggt
     1501 gcggccgttg cgtggatgca tgtcccatga gactcgtgcc gcagcggctg gcgctgggca
     1561 gcaggaacag aaactgggat gtcgtcaaaa aatacaacat ccttagttgc gtcgagtgcg
     1621 gctgctgtgc ctacgtatgt ccggctggca tagaactggt gcaact
//
LOCUS       DAONFI010000407         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_102868_length_1666_cov_4.541279, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000407 DAONFI010000000
VERSION     DAONFI010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102868_length_1666_cov_4.541279"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..673
                     /gene="lptE"
                     /locus_tag="PLK08_10285"
     CDS             137..673
                     /gene="lptE"
                     /locus_tag="PLK08_10285"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPS53929.1"
                     /translation="MNKKLLTVVVSCVVASFAALSGCSSDPNSGYTGKSMYRTNIKTV
                     AVPMATRGEDVYRRENEMRLTEALVKRIELNTPYKVTVQSRADTTLTCSIEQINQAVL
                     SYNPDTGDPRDISVTFVVAFKWVDNRTGKILAQEKNYPITGTYISDSPFNEKFFQGSE
                     TVINKVAERIVEHMERDW"
     gene            732..>1666
                     /locus_tag="PLK08_10290"
     CDS             732..>1666
                     /locus_tag="PLK08_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPS53930.1"
                     /translation="MPEENNNQNTKPPLPSEKSDETKPSGDPQLPQQLRNLRPPMPPK
                     GQRGFMGAMLIIMLLLFGMMFYNDMVNPKRKISMTQFWTYVDNNQLTGELKVHPDSIE
                     GRVANVPLAKDQPAAFYVDYRYETDQNFSEQLMVRLRKAGSKVEVVYPPASWWSTGML
                     QNLIFTVVLFGLLWFFLFRKIGTAGGAGFLGNFGRSRHRMMTKEQSKIKFSDVAGIDD
                     AKDEVAEIVEFLKNPGRFSRLGGRVPRGVLLIGPPGCGKTMLAKAIAGEADVPFFSIS
                     GSDFVEMFVGVGASRVRDLFKQAKDPSPCIIFLDE"
BASE COUNT          427 a          423 c          441 g          375 t
ORIGIN      
        1 tgatgaaata aacaggacaa tggaaacgca gccgacacct gcgggtgaaa acaaaacttc
       61 cgattcagaa tcgccgatac ggctggaaga cctccgcaag agactgcgta acaataattg
      121 atgaggtatc aacaacatga ataaaaaact tttgacagtc gtcgtttcat gcgtcgttgc
      181 ttcgtttgcc gcactgtccg gctgcagcag cgacccgaat tccggttata ccggcaaatc
      241 catgtatcgc accaacataa aaaccgttgc cgtgccgatg gctacccgcg gcgaggacgt
      301 ttatcgccgt gaaaatgaaa tgcggctgac ggaggccctc gtcaagcgaa tcgaactcaa
      361 tacgccgtac aaggtcaccg tgcaaagccg tgccgatacc actctcactt gttccatcga
      421 acagattaat caggcggttt tgagctataa ccccgatacc ggcgatcctc gcgatatcag
      481 cgtgactttc gtcgtcgcgt tcaaatgggt ggacaaccgc actggcaaga tattggcgca
      541 ggaaaaaaat tatccgataa ccggtacgta tatttcggat tcgccgttca atgaaaaatt
      601 cttccagggc agcgaaaccg tgataaacaa ggttgcagaa agaatcgttg aacacatgga
      661 acgcgactgg tagaattttt ctatgcgtcg cgaagaatca taataaacgt aaatttacgg
      721 attacacccg catgccagaa gagaacaata accagaatac gaaaccgccg ctgccgtcgg
      781 aaaagtccga cgaaaccaaa ccttcaggag acccgcagct gccgcagcag ctcaggaatc
      841 ttcgtccgcc catgccgcct aaaggccagc gtggattcat gggggccatg ctgattatca
      901 tgctgctgct tttcgggatg atgttttata acgatatggt caacccgaag cgtaaaatca
      961 gcatgacgca gttctggact tatgtcgaca acaatcagct taccggcgag ttgaaggttc
     1021 atcccgacag cattgagggc cgcgttgcga acgtcccgtt ggcgaaggat cagccggcgg
     1081 ctttttatgt tgattaccgt tatgagaccg accagaattt cagcgaacaa cttatggtgc
     1141 gtctgcgaaa ggccggctct aaggtcgagg tggtttatcc gcccgcaagc tggtggtcga
     1201 cgggcatgct gcagaatctg atatttacag tggtgctgtt cgggctgttg tggtttttcc
     1261 tgttccgcaa aattggcaca gccggcgggg cgggcttttt gggcaacttt ggccgcagcc
     1321 gccatcgcat gatgaccaag gaacagtcga aaatcaaatt ttccgacgtc gccggcattg
     1381 acgacgccaa ggatgaagtt gcggagatag tggagtttct caaaaatccc ggccggttca
     1441 gccgtctcgg cggccgtgtg ccgcggggcg tattgcttat aggccctcct ggatgcggca
     1501 agaccatgct cgcaaaagcc atcgccggcg aagccgacgt tcccttcttc agcatttccg
     1561 gcagtgattt tgtggaaatg ttcgtcggtg tgggtgcatc gcgtgtgcgt gacctattca
     1621 agcaggccaa agatccctcg ccgtgcataa tatttctgga tgaaat
//
LOCUS       DAONFI010000408         1637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_105904_length_1637_cov_3.454488, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000408 DAONFI010000000
VERSION     DAONFI010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105904_length_1637_cov_3.454488"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PLK08_10295"
     CDS             <1..1466
                     /locus_tag="PLK08_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPS53931.1"
                     /translation="TDRLARIIEKRVEKFRKSATPGQIDNFKNIVANVGTSSGNIGSS
                     SSDSGSHTADLTMVFPDYEDRIKPSAEVIADVRHELEDFPGAEVKVEKRKEGPPTGSA
                     VTVRIIGEDMGLLEKLSEEVAAKIKGTPNMVNMRSDLEAAKPELIFIPDRVQAAILET
                     DTNSISQFLKTAIYGTKVGEYQQFNDDYDIRLRPAVSKRRGVDDLLKLRVPTLDGGAV
                     PLTSLGQFEYRPGLGTIHRINRKRVVTITADAEGRPGLEVLADVQKKLADMQLPAGYR
                     IEYAGEKEEDDKARAFLSKAFIIALLLIVAILVAQFNTLSVPLIIMSTVILSLIGVFV
                     GLLIFRQPFGVVMTGVGVISLAGVVVNNAIVLLDYTRQLQQRGYTLKDAAVEAGITRL
                     RPVLLTAGTTVLGLVPMVTGISFDFHTMSIAWRSSSSQWWSSMAVAVIFGLGCATMLT
                     LLVVPSLYVLTSKIAEKMGMNTAPQIHHKSMPSYEDF"
BASE COUNT          406 a          408 c          447 g          376 t
ORIGIN      
        1 aaacggacag gctggctcgc ataatcgaaa aacgcgtcga aaaatttcgc aaatccgcga
       61 cgccgggcca gattgacaat ttcaagaata tcgtggctaa tgttggtacg agcagcggca
      121 acatcggcag ttcatcgtcc gattccggct cgcatacagc cgacctgacg atggtatttc
      181 ccgattatga agaccgcata aaaccatctg cggaagtgat agccgatgtc cgccatgaat
      241 tggaagattt cccgggtgcg gaggttaagg tcgaaaaacg caaggaaggc ccgccgaccg
      301 gctcagccgt gacggtgcga atcataggcg aggatatggg cctgctggaa aaactcagtg
      361 aggaagtcgc cgcgaaaata aagggcactc ccaatatggt caacatgcgc agcgacctgg
      421 aagccgccaa acccgaactg atattcatac ccgaccgtgt gcaggctgcc atccttgaaa
      481 ccgacacgaa ttcgatttcc caatttctga aaaccgcgat ttacggaacg aaggtcggcg
      541 agtatcagca gtttaatgac gattacgaca ttcgtttacg gccggcggtt tccaagcggc
      601 gcggcgtaga tgatctgctc aaattgcgag tgccgactct cgacggcgga gctgtgccgc
      661 tgacttcgct tggacaattt gaatatcggc ctggcctggg cacgatacac cgcatcaacc
      721 gcaaacgcgt ggtcacgata accgccgacg ctgaaggccg gcccggcctg gaagtgttgg
      781 ctgacgtgca gaaaaaactc gccgatatgc aactgccggc tggctatcgc atagaatacg
      841 ccggcgaaaa ggaagaagac gacaaggcac gcgccttcct gtccaaggca ttcataatcg
      901 cactgctgct catagtggcc atactcgtcg cgcagttcaa cacgctctcc gtgccgctta
      961 tcatcatgtc tacggtcata ctttcgctta tcggcgtttt cgttggcctg cttatattcc
     1021 gccagccgtt tggcgtggtg atgacgggtg tgggcgtaat cagcctcgca ggcgtcgtcg
     1081 tcaacaatgc cattgttctg ttggattaca ccaggcagtt gcagcagcgc ggctacacgc
     1141 tcaaggatgc cgcagtcgaa gcgggcataa caagacttag gccagtactg ctgaccgcag
     1201 gaactaccgt gctgggtctg gttccaatgg ttacaggaat cagcttcgat tttcatacca
     1261 tgtccattgc atggcggagt tcgtccagtc agtggtggtc aagcatggcg gtggcggtta
     1321 tcttcggcct gggctgtgcg acaatgttga cgctgctggt tgtgccgtcg ctgtatgttc
     1381 tgaccagtaa aattgccgaa aaaatgggta tgaacacggc gccgcagata catcacaaat
     1441 ccatgccgag ttacgaagat ttttgaaatg tgatggaatt tttatgcgaa ctttttgaaa
     1501 tattaatctg tgaagcataa aattaaaaag gtggagaatg gccaccaaag tattattatc
     1561 agacggtgat tgtaaaatct gcttttagag catattaaac ttgactttgc gcttgttgct
     1621 gtttgcggcg tcggctg
//
LOCUS       DAONFI010000409         1624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_107272_length_1624_cov_3.286807, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000409 DAONFI010000000
VERSION     DAONFI010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107272_length_1624_cov_3.286807"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLK08_10300"
     CDS             complement(<1..252)
                     /locus_tag="PLK08_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPS53932.1"
                     /translation="MSITIGKFKFENPLFVGTGKYATYELMAEALDACGCQVVTVAVR
                     RERMLDAGGRNILDFINPAKYTLLPNTAGCFCADDALRNA"
     gene            complement(302..592)
                     /locus_tag="PLK08_10305"
     CDS             complement(302..592)
                     /locus_tag="PLK08_10305"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPS53933.1"
                     /translation="MPMTKKQLEKMSRIYHMMSDPSRLKIVLCLSKNETNVTALCKKL
                     HMSQPTVSRHLSLLKLTGIAEARRNGKEIYYSILPESRKTLNVIIAKGMELI"
     gene            complement(744..1343)
                     /locus_tag="PLK08_10310"
     CDS             complement(744..1343)
                     /locus_tag="PLK08_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53934.1"
                     /translation="MTGLLFATSLFCLYKSYTAHSILSPYVIGTYRLIATIFCSSLAF
                     KNGIPPHAVLAGIILGTYSASISRIGQERFRQTIRKLGIFAWWPTILAGIVGISCVVR
                     NFAFYGATAPAWMSGYMGVWLLFRTFYISNAVKGWCTPSRLFQADGLLVRGMIPLQAA
                     VFMLFATSQPWNAIAAAILAMILSTFQTIACKLLPTPEF"
BASE COUNT          431 a          350 c          403 g          440 t
ORIGIN      
        1 tgcattacgg agggcatcat ccgcacaaaa acatccggcg gtattgggca gaagggtata
       61 tttggcggga ttgataaaat cgagtatgtt tcggccgccg gcgtccagca tgcgttcacg
      121 gcggacggcg acggtgacga cctggcagcc gcaggcgtca agggcttcag ccatcaattc
      181 gtaggtggcg tattttccgg ttccgacgaa aagcggattc tcaaacttaa acttccctat
      241 cgtaatgctc atcttacaaa acctcttctt ccggcaggga ttgggtataa tgtcgtataa
      301 tttatataag ttccatgcct tttgcaataa tcacgttcaa tgttttgcgg ctttctggca
      361 gaatggaata atatatttcc ttgccgttgc gtcgggcttc tgcgatgccg gtaagtttga
      421 gcaggctgag atgccgcgaa accgtcggct gactcatgtg cagtttcttg cacagtgcgg
      481 tgacgttggt ttcattcttc gacaggcaga gaactatctt aagccgcgaa ggatcgctca
      541 tcatgtgata tattcgcgac attttttcaa gctgtttttt ggtcatcggc attttatatc
      601 tcctggaagt taaacatatc aacacaatag aataagaaaa attaaatcaa agtccagatt
      661 tttttatgct taatgtatcg ccgtttaaat attatcacgg atttgtaagt tctgaaatga
      721 agtaaggacg atatgcttat cattcagaat tcaggtgtgg gcagcagctt gcaggcgata
      781 gtctggaagg tcgacagtat catcgccaga attgccgccg caatggcatt ccacggttgt
      841 gacgtcgcaa acagcatgaa gacggcggcc tggagcggta tcatgccgcg taccagaagg
      901 ccgtccgcct ggaaaagtcg cgacggcgta caccagcctt ttacggcatt tgatatataa
      961 aacgttcgga ataacaacca cacgcccata tatccgctca tccatgccgg tgccgtagcg
     1021 ccataaaacg caaaatttct tactacgcat gatattccga caatgccggc tagaatcgtg
     1081 ggccaccatg cgaaaattcc cagttttcgt atggtctgac ggaatctttc ctggccgatt
     1141 cggcttattg aggcggaata tgtgcccagt atgatgcccg cgaggacggc gtgcggcgga
     1201 atgccgtttt taaaggcgag agacgaacag aaaatcgtgg caataagtct gtatgttcca
     1261 atgacgtatg gcgacagaat cgaatgagcg gtataactct tatacaggca aaacagcgat
     1321 gtcgcgaata gaagccctgt catggaagcg agggttgtac caagaggcgt aaaattttcc
     1381 gaagggcctg cttgtaacgc caaaatctct tgtcaatgtg acgagagaag tagcattatt
     1441 gctaaattat cagggcattg caagcgttcg gtgtcgtttt tgcattgctc aggaagagat
     1501 acaaagttag ctttaacttt aatagaacac ccctgccaat atgaaaattt gcaatcgttt
     1561 taggtttttt ttctttcaga ttcgtggctt tttggagcgt gcaaaaaaaa atggtaagtt
     1621 tact
//
LOCUS       DAONFI010000410         1617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_108061_length_1617_cov_3.822023, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000410 DAONFI010000000
VERSION     DAONFI010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108061_length_1617_cov_3.822023"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLK08_10315"
     CDS             <1..290
                     /locus_tag="PLK08_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPS53935.1"
                     /translation="DEELIKLESAAAGDDDGFEMERDELFDESVEIILSARRGSVSLL
                     QRRLQIGYGRASRIIDQMAAAGILGDHKGSQARECLMTLDEWLEMKEATGE"
     gene            319..843
                     /locus_tag="PLK08_10320"
     CDS             319..843
                     /locus_tag="PLK08_10320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPS53936.1"
                     /translation="MIIRQEEPKDFSRIYELVKTAFQTAKVSSGEEQDLVERLRKSSG
                     YIPELAMVAEEEDGRLVGYIMLTRMYVHGDDGKKYPTLLIAPLAVAIEYRDKGIGSAL
                     VKESLWAAAELDYRSVFLVGDPKYYKKLGFRAARKFGITHPGIPAEYVMACELSPDAL
                     KGIHGTVDCRGLDE"
     gene            1251..>1617
                     /locus_tag="PLK08_10325"
     CDS             1251..>1617
                     /locus_tag="PLK08_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPS53937.1"
                     /translation="MIKKRLFTPGPTEVPAEVLVEMAKPIFHHRTSQFKEMFAKVNEG
                     LKKIYRTKNDVLTIAGSGTAGMEAAISCAVPRDKKVLVANSGKFGERWVKVAKLYKLD
                     VDEVVTEWGTPITAQTVKEN"
BASE COUNT          434 a          390 c          456 g          337 t
ORIGIN      
        1 tcgatgagga actcataaaa ctcgaatctg cggcagccgg cgacgacgac ggatttgaaa
       61 tggaacgcga cgaacttttt gacgaatctg tggaaatcat actttccgcg cggcgcggca
      121 gtgtgtcgct gctgcagcgc agattgcaga taggttatgg ccgagccagc cgcataatag
      181 accagatggc tgccgctgga atactcggcg accacaaagg ctcgcaggcc cgcgaatgtc
      241 ttatgacgct cgacgaatgg ctggaaatga aagaagccac cggcgaatga gacgattgag
      301 aatgacagga atgaaaccat gattatcaga caggaagaac ccaaagattt tagccgtata
      361 tatgaattgg taaaaacggc gtttcagact gcaaaagttt ccagcggcga agaacaggat
      421 ttggttgagc ggcttcgtaa aagcagcgga tacattcctg agttggcgat ggtggctgaa
      481 gaagaggacg gcagactggt gggctacata atgctgacca ggatgtacgt gcacggcgac
      541 gacggaaaaa aatatccgac gcttttaatt gcgccgctgg cggtcgctat tgaatacagg
      601 gacaagggga tagggtctgc cctcgtcaag gaaagcctct gggctgccgc cgagctggac
      661 taccgttccg tatttctcgt cggcgacccg aaatactaca aaaaattagg attcagggcg
      721 gccagaaaat tcggcattac gcatcctggc ataccggccg aatacgtgat ggcgtgcgaa
      781 ttgagtcccg atgcgctgaa aggcattcac ggcactgtcg attgtcgcgg cctggatgaa
      841 tgatttttgg cggcgcggca gttatgatgg ttcatatttt tggataggtt ctaaaaactt
      901 tcgaaaactg cgataaaaaa tccacttgac ggttttattc aggcgggata aaatttatct
      961 ttccaatcgg ttgcggacag atttccaagc cgtctgcaac ggtggcccat gattcgattt
     1021 gcgagcggga ttcgcagagt cgatggcttg ttcagggttc gccgcattca ggcttggcat
     1081 atcgccgcag ctatcccaca attgaatatt tttttaaaaa atgataaggc aatcggtggc
     1141 aaccgtggca aggggctgcg gcaatgccgg ctagtaccga gtgcataacc cttttgggtc
     1201 ggctgaaaag tttttgcaga aactcctgcc gccccagcaa ggagatagaa gtgattaaaa
     1261 aacgtctctt cacccccggc ccgacggaag tcccggctga agttctggtc gaaatggcta
     1321 agccgatttt ccaccaccgc acgtcgcagt tcaaggaaat gttcgccaag gtcaacgagg
     1381 gcctcaaaaa aatatacagg acgaagaacg acgtcctcac aatcgccggc tcaggcactg
     1441 ccggcatgga agcagccatc agctgcgccg tgccgcggga caaaaaagtc ctcgtcgcca
     1501 acagcggcaa attcggcgaa cgctgggtca aggtggccaa gctctacaag ctcgacgttg
     1561 acgaggtagt caccgagtgg ggcacgccca taacggccca gaccgtcaaa gaaaatc
//
LOCUS       DAONFI010000411         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_108740_length_1611_cov_2.926093, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000411 DAONFI010000000
VERSION     DAONFI010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108740_length_1611_cov_2.926093"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /gene="pheS"
                     /locus_tag="PLK08_10330"
     CDS             complement(<1..891)
                     /gene="pheS"
                     /locus_tag="PLK08_10330"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPS53938.1"
                     /translation="MSQIDELKKLQSNAMSELAAMNSAEALEQWRIKYLGTKGLVKAA
                     MGALKDLPGEIKGQYGKTANQMKNELTAAFDAAKTSVSSATSSAAPKEDVTLPGRKPR
                     IGHIHIITQTIRELTDIFGRMGFETVYGPEVEDEKHNFIDLNIPESHPARDPLDNFYI
                     DVETMLRSQTSTVQIRTMETRKPPIRVVAPGRVYRPDAVDATHSFMFYQLEGLCVDEG
                     ITMVDLKTSVDQFCKAFFGEDVETRFRPSFFPFTEPSAEVDMLFRFKDGRTQWIEMGG
                     CGMVDPNVLNTVGIDPEKYTG"
     gene            complement(1064..1137)
                     /locus_tag="PLK08_10335"
     tRNA            complement(1064..1137)
                     /locus_tag="PLK08_10335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1101..1103),aa:Ile,seq:cat)
     gene            1274..>1611
                     /locus_tag="PLK08_10340"
     CDS             1274..>1611
                     /locus_tag="PLK08_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPS53939.1"
                     /translation="MIFYGIILGLIAAFSQSMSYVFARQFSSGRTSPQARLLVASHMA
                     MGIAGLVILPLVWPAGIHWDWHWGRPLILCAVFYLLGQGLMFVALHAAEASKISPLLA
                     LKVVMLAFLT"
BASE COUNT          326 a          372 c          451 g          462 t
ORIGIN      
        1 gcctgtgtat ttttccgggt ctatgccgac cgtgttcagc acattcgggt ctaccattcc
       61 gcagccgccc atctctatcc actgcgttcg gccgtctttg aatctgaaga gcatgtcaac
      121 ttcagccgac ggttcggtaa acgggaaaaa acttgggcgg aatcgcgttt cgacgtcttc
      181 gccgaagaag gctttgcaga actggtcgac gctggttttg aggtctacca tcgttatgcc
      241 ctcgtccacg cagaggcctt caagctgata gaacatgaaa ctgtgcgtgg cgtcgactgc
      301 gtcggggcga tatactctgc caggtgccac cacgcggatt ggcggcttgc gtgtttccat
      361 cgtgcgaatc tgtacagtcg acgtctgtga gcgcagcatg gtttccacgt ctatgtaaaa
      421 attgtccaac gggtcgcgag ccggatgtga ttcagggata ttcaggtcga tgaaattatg
      481 tttttcatct tcgacttcgg ggccgtaaac ggtttcaaaa cccatccggc cgaaaatatc
      541 ggtcagttcg cggatggttt gcgtgatgat atgtatgtgg ccgatgcgcg gcttgcggcc
      601 gggaagggtt acgtcttcct tcggcgcggc ggacgatgtg gcggagctga ccgatgtttt
      661 ggcggcgtcg aaggcggctg tcagttcatt tttcatctga ttggcagttt tgccgtattg
      721 accttttatt tccccgggca ggtctttcag ggcgcccatg gcggctttta cgaggccttt
      781 cgtgccgaga tattttatac gccactgttc aagagcctcg gctgagttca tcgccgcaag
      841 ttcagacatg gcattgcttt gcaatttctt caattcgtca atctgagaca tttttctttt
      901 ccttactgac aggcctgcaa agtatagcgt ttttgcttga cggaagcaag ccggcggctg
      961 aagttgtcga cgaattggcg gttaaagcaa tatgtacaga ccggagttct gcgcataaaa
     1021 aaactccgta ttttcaggtt acggagtttt tttagtgaat taatgggcgc tgagggactc
     1081 gaacccccga ccaaaagatt atgagtcttc tgctctaacc aactgagcta agcgcccaag
     1141 gggatggtta atgtaacttt gggacggcgg agtgtcaaac aaaaatttag gacgataaag
     1201 attgttattt tgattgcatg ttctgccggc aaaggggtat gatacccgtt ttttatgagg
     1261 tttttgagac aatatgatat tttacggaat aattctcggc ttgattgcgg cattttcgca
     1321 gtcgatgtcg tatgtttttg cccgccagtt ttcctctggc cgtacaagcc cgcaggctag
     1381 attgctcgtc gccagccata tggctatggg gatagcaggg ttggtgatac tgccgctggt
     1441 ctggccggct ggcattcatt gggactggca ctggggccgg ccgcttattt tatgtgcggt
     1501 gttttatctc ctcggccagg ggttgatgtt cgtagcattg catgcggcgg aagcgtccaa
     1561 aatctccccg ctgttggcgt taaaagtagt catgctggcg tttttgacat g
//
LOCUS       DAONFI010000412         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_114909_length_1558_cov_3.200266, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000412 DAONFI010000000
VERSION     DAONFI010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114909_length_1558_cov_3.200266"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..158
                     /locus_tag="PLK08_10345"
     CDS             21..158
                     /locus_tag="PLK08_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53940.1"
                     /translation="MEYITQRYTTDLENVIRRAPEQYLWTHRRWKHRPKGEEQPADGI
                     A"
     gene            608..>1558
                     /locus_tag="PLK08_10350"
     CDS             608..>1558
                     /locus_tag="PLK08_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53941.1"
                     /translation="MLKKSSYSVIGLACVAIWTLGCLSGCSDSKENIARKELVNATRL
                     SDEFCRAASAEMVNPLADANNGQIAEFLDKARQQLTAAINKNAQYAGELTEELAMARA
                     SLAQIENVEAQYKFLLASRQMIVCDSMMNEIFNLFSVARATASAAKAAKKQTEFDSAT
                     FTAKIDQNAQQVKATKNRLQNIKAALDEKKDSLDKLTAEMNDKSLQAQRLMRESQLLP
                     AGKSGSKLDQALKLNDEIGKIQKSVTVGEGELAGLAMLIESLELKIKNQEEYGKSLDD
                     SQKIMNDLAIEAKARADVLDTAATAAHKRLAEKCSEFNTLQ"
BASE COUNT          461 a          400 c          369 g          328 t
ORIGIN      
        1 ggaaaggcgt cgataagccg atggaatata taacccagcg ttataccacc gaccttgaaa
       61 acgtaatccg ccgcgcgccg gaacaatatc tctggacgca tcgccgttgg aagcatcggc
      121 caaaaggcga agaacagccg gctgacggca tagcttgaag aaaaattcga atttcgggct
      181 caggcttacc gaatggctct cgaagttaca gtcaatgccc cgtcgcttcg atttatcatg
      241 gttatcgcca ggatatgcag taattaatgg tagcttgaat ctccggtcat acctttgcgg
      301 atataaaatg ccggaacatc cacattattt tcaagaaccg ctcaagtagc tcgccacccc
      361 tgttgtctga atttacccaa aacacaaact gtctttgaaa attttatatt ttttagagca
      421 gttcaagaaa tcgcctggcc attcacgccg ccactcgatg accgccgccg aaacaggttt
      481 tctgtcatat tttgttgcaa aagaggtttc gggctgttac attttttcat agaacggttt
      541 tcaaaatgac tttttgggaa ctgctctaat tcgttattta acgttcgtct taataggaga
      601 catcgccgtg ttaaaaaaat cctcttacag tgtaattggt ctggcttgcg tagccatctg
      661 gacacttggt tgcctttctg gctgcagcga cagcaaggaa aatattgccc gcaaggaact
      721 ggtaaacgcc acacggctgt ccgacgagtt ctgtcgtgca gcctctgctg aaatggtcaa
      781 cccgctggcg gacgcaaaca acggtcaaat cgcagaattt ctcgacaaag cccgccagca
      841 actcacggca gctataaata agaacgccca atatgccggc gaacttacgg aagaactcgc
      901 gatggccagg gcgtctctgg ctcagataga aaacgtcgaa gcgcaataca aatttctcct
      961 ggccagccgg cagatgatag tctgcgattc gatgatgaat gaaatattca atctcttcag
     1021 cgtcgccagg gcaaccgcat ctgccgccaa agctgccaaa aagcagaccg aattcgactc
     1081 agccacgttc acggcgaaaa tcgaccagaa tgcccagcag gtgaaggcga cgaagaatcg
     1141 tcttcaaaat ataaaggctg cactcgacga aaagaaagac agcctcgaca aactcacggc
     1201 tgaaatgaac gataaatccc tgcaggcaca gcggctgatg cgtgagagcc agcttcttcc
     1261 agccggcaag agcggctcaa aactcgacca ggccctgaaa ctcaacgacg aaatcggcaa
     1321 gatacaaaaa tccgtcaccg tcggcgaagg cgaactggct gggctggcga tgctgataga
     1381 atcgctcgaa ctgaaaataa aaaatcagga agaatacggc aaatcgctgg atgactcgca
     1441 gaaaatcatg aacgacctgg cgatagaagc caaagcacga gccgacgttc tggataccgc
     1501 cgcaacggca gcacataagc gactcgccga aaaatgcagc gagttcaata cgctgcaa
//
LOCUS       DAONFI010000413         1538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_117316_length_1538_cov_7.034390, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000413 DAONFI010000000
VERSION     DAONFI010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117316_length_1538_cov_7.034390"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1081)
                     /locus_tag="PLK08_10355"
     CDS             complement(338..1081)
                     /locus_tag="PLK08_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPS53942.1"
                     /translation="MKLRRSILTNLHPAQMQRHVKPRLAISPQVFRGFTLIELLVVIA
                     IISLLVSILMPSLNKAKDLARQVTCSANIKNINLGFNLYINDWEGYFPLVRETTAPTG
                     TWADKLYNDYIKDVNVFMCPSVPERTFTPNVSQGARPMAYGMEWWLGGGANITTSGWK
                     GRRLIDIANPSMTVLLGENNNSSTYHGYGLHNYLWPDNIIYQWGWPDDERHSGVSNIL
                     FVDGRIAPYTREEVCGDFFWYTQTNWMNS"
     gene            complement(1103..>1538)
                     /locus_tag="PLK08_10360"
     CDS             complement(1103..>1538)
                     /locus_tag="PLK08_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53943.1"
                     /translation="EGTKRPDVSFKDGVVKIGKNREGLYMRKGEKTDGDCAFVGKEDG
                     AINRILLVAGSRISDAVQLDVFADFMIVTRQGESLDIQLMESSSKKVNRIELPGLQGV
                     NKVTVNGKTCKISETDGKMVIAGPFEKISPNPDNLDLFWIVK"
BASE COUNT          455 a          390 c          277 g          416 t
ORIGIN      
        1 ttctgccgct gattcgattt ccattatgtt cctttcctgc aggataaaac tacaaaacca
       61 caatacaatc ttgaaagtaa tataaaaaaa acctggcact caccacatat actaatatgg
      121 catgcctatg cagagcaaat atgaaacaga ccaagtaccc aaaacaagtt tgaggtataa
      181 aaaataccga cacattgctt ttacataaca ctacacacgg tatttctata tataatcaag
      241 aaattcttac acgtcaaaca aaaattgata taagaactta tttttgatta cccaagccag
      301 ggaaattccc ctcacaaacc cccaaatata ttattgttca cgaattcatc caattagtct
      361 gggtatacca gaagaaatca ccgcaaacct cttcacgcgt atacggagca atacggccgt
      421 cgacaaaaag tatattcgaa acgcctgaat gacgttcgtc atccggccaa ccccattgat
      481 aaataatatt atccggccac aaataattat gcaaaccata gccatggtat gttgaactat
      541 tgttattttc acccaacaag acagtcatcg acggattggc gatatcgatg agccgacgcc
      601 ctttccaacc tgatgtggtt atgttagcac ctccgcccag ccaccattcc ataccgtaag
      661 ccatcggcct cgctccctgg cttacattcg gcgtaaaagt cctctccgga acactgggac
      721 acataaaaac atttacgtct tttatgtaat cattatataa cttgtctgcc cacgtaccgg
      781 taggtgcagt agtttctcgt accaacggga aatagccttc ccaatcatta atatacaggt
      841 taaaccctag atttatgttt ttgatattcg cagagcaggt aacctggcgg gccaggtctt
      901 tggctttatt caagctgggc atcagaatcg ataccaatag cgatataatc gctatcacta
      961 ccaacaattc tataagagtg aacccacgaa agacttgcgg agatatggca agtcggggct
     1021 taacatggcg ttgcatttgc gccgggtgca gattcgtcaa tatagaacgt cgcagtttca
     1081 taatctgcct ccatctatta ttttacttca caatccagaa taaatcaaga ttgtcggggt
     1141 tgggagagat tttctcgaac ggcccggcaa tcaccatttt gccgtcagtt tcggatattt
     1201 tacaagtttt gccgttcacc gttactttat tgacgccttg caatccaggc agctctattc
     1261 gattgacttt tttggacgaa ctttccatca actgtatatc cagcgactcg ccctgccgcg
     1321 tcacaatcat gaaatcggca aaaacgtcga gttgaaccgc gtcagatata cggctgccgg
     1381 caaccagaag tatacgatta atcgctccat cttctttacc gacgaacgca cagtcgccgt
     1441 cggtcttctc gcctttacgc atatagagac cttcgcgatt tttaccgatt ttgaccacac
     1501 cgtccttgaa ggaaacatcc ggccgcttcg taccctca
//
LOCUS       DAONFI010000414         1532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_118113_length_1532_cov_4.330399, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000414 DAONFI010000000
VERSION     DAONFI010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118113_length_1532_cov_4.330399"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1282)
                     /locus_tag="PLK08_10365"
     CDS             complement(203..1282)
                     /locus_tag="PLK08_10365"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPS53944.1"
                     /translation="MKSMKAESFKFLESLLTSCSPSGFEASTQKIWCDYVRKFADEVH
                     TDNYGNAVAVLNPGADTKILVDGHIDEVGLMIKYIDDKGFIYFQSIGGVDPALIRSKR
                     VNIHTGKGIVRGVVGATAIHLIPKDQEQKSPKMHELFIDIGAKDAKEAKRKVSLGDPI
                     TFVDSFEMLDENIAVSRALDNRIGCWVAAEVLRIASVSRDKLNCTLYACSSIQEETGC
                     NGAQMNVMNVKPDAAIAVDVTHATDTPGIDVKLHGEVKLGGGPTYTVGRENHPVLIDR
                     LKKVAARKKIELQTEIFSKTGGTDAMVMWTKNGGTPSAIVSPPTRYMHSTIEMMDLRD
                     LQKTADWLAAFVIDVKKGEKFKVKV"
     gene            complement(1321..>1532)
                     /locus_tag="PLK08_10370"
     CDS             complement(1321..>1532)
                     /locus_tag="PLK08_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53945.1"
                     /translation="LCLCCCVLNWQAALLTGPFLLMFAFGFFYVAAMTSKAMKAPKVN
                     RELEAQHAGCKKSVSDDNTEVEPAM"
BASE COUNT          375 a          394 c          339 g          424 t
ORIGIN      
        1 aaattaaaaa acaggttcgc cgcaaaggct atggcatcac attcatgttg ttgtaaccat
       61 aaataaaaag tgcttttttt atttttaaag caattcttga aaataccctt tggcaactcg
      121 tggcattctg taccgcatgg aagcaataaa aaagccccga attaacgggg cttttttgat
      181 tattcagagt ggttcgcaac tgtcagacct tgactttgaa tttttcacct ttcttgacat
      241 cgatgacgaa cgcagccagc caatctgccg ttttttgcaa atcgcgtaaa tccatcatct
      301 caatcgtaga gtgcatatat cgcgtcggcg gcgaaactat cgccgacggc gtaccgccat
      361 ttttcgtcca catcaccatc gcatccgttc cgccggtttt cgaaaaaatc tctgtctgta
      421 attcgatttt cttccttgct gccaccttct taagccggtc tatcagtaca ggatgatttt
      481 ctctgccgac ggtatacgtc ggcccgccgc caagtttcac ttcgccgtga agttttacat
      541 ctatacctgg cgtatcggta gcgtgggtca cgtcaacggc aatagcggcg tccggcttga
      601 cgttcatgac gttcatctga gcgccattgc agcctgtctc ttcctgaatg ctgctgcacg
      661 cataaagggt gcagttgagt ttgtcgcggc tgacggaggc tatacgcaaa acctcagccg
      721 caacccagca gccaatacgg ttatcaaggg cacgtgaaac ggcgatgttc tcatcaagca
      781 tctcaaagct gtccacgaat gttatcggat cgcccaatga aacttttctc tttgcttcct
      841 tggcgtcctt tgcgccgata tcaataaaca gctcgtgcat cttcggagat ttctgttcct
      901 ggtctttggg tataaggtgt atcgcggtcg cgccaaccac gccgcgaact attcccttgc
      961 cggtatgtat gttcacgcgt ttggaacgaa tgagcgctgg atcaacgccg ccaatgctct
     1021 ggaaataaat aaaccccttg tcgtcgatat atttaatcat gaggccgact tcatcaatat
     1081 ggccgtcgac taaaattttg gtatccgcgc caggattgag cactgccaca gcattgccat
     1141 aattgtcggt atgcacttcg tcggcaaatt tacggacata gtcacaccag attttttgcg
     1201 tggatgcttc aaaaccgctc ggcgaacagc ttgttaaaag cgattcaaga aatttgaacg
     1261 attccgcttt cattgatttc atattaacgc tccattatat gtccggcggc aaccattatg
     1321 ctacattgca ggttcgactt cagtattgtc atcggataca cttttcttgc agccggcatg
     1381 ctgtgcttcc agttcacggt tgactttcgg ggctttcatg gcttttgagg tcatagctgc
     1441 aacatagaaa aaaccgaagg caaacatcag caggaacggc cccgtcagca gcgcagcctg
     1501 ccagttgagc acacagcagc acaggcaaag ca
//
LOCUS       DAONFI010000415         1531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_118248_length_1531_cov_3.713415, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000415 DAONFI010000000
VERSION     DAONFI010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118248_length_1531_cov_3.713415"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLK08_10375"
     CDS             complement(<1..634)
                     /locus_tag="PLK08_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53946.1"
                     /translation="MSSRLQRINSSVTKNRKFKGKSLRRYKQWHSSFVEGRDVDAIAG
                     DRQKFSGRAVKLREDSFCDSAADLEHLKKVAGLVTGVFQRGVLVQCDGFAKDDLFCSL
                     AKTYRPPEGSENYSPLAIGDDVTVALIPEEHADGQLGMDRNRMDGMILSRSPRRSLLA
                     RPQPRSRKRNNEYADDTQIKVIAANMDTLLIVSAVEQPKFRPGLVERFLIA"
     gene            664..1284
                     /gene="coaE"
                     /locus_tag="PLK08_10380"
     CDS             664..1284
                     /gene="coaE"
                     /locus_tag="PLK08_10380"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPS53947.1"
                     /translation="MNDSVKSVPVIGLVGGVGSGKSAAADIFRSLGCEVIDADAVGHE
                     VLQLPQVAAELGKIFGRQIFDAENRISRPALAKKVFGCRNAENLHRLEAVVHPVMWER
                     FMAAIRDAANKGVIAVVLDAAVLFEAGWDSLCSCTIFVDSAADVRRRRVLQTRGWDEN
                     KWRQRENSQFSLDKKAAQCCYKLYNDSDIPHLKLQVKSLLDQITGN"
     gene            complement(1337..>1531)
                     /locus_tag="PLK08_10385"
     CDS             complement(1337..>1531)
                     /locus_tag="PLK08_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPS53948.1"
                     /translation="FLLVCFVRGCVRIFLGISPNMPHFPSLPRTNPLAQKQELSTFQC
                     SKPSAFIAKTQKACKPEELK"
BASE COUNT          329 a          370 c          433 g          399 t
ORIGIN      
        1 cggcgatgag aaaccgttcg acgaggccgg gccggaattt cggctgttcg acggctgaga
       61 ctatcagcag cgtgtccatg tttgcagcga tgacttttat ctgtgtatcg tctgcatatt
      121 cgttattccg tttgcggctg cgcggctggg ggcgggccag cagggaacgc cgcggactcc
      181 gcgagagtat catgccgtcc atccggttgc ggtccatgcc cagttggccg tcggcgtgct
      241 cttcgggtat aagtgcgacg gtgacgtcgt caccgatggc cagcggcgaa taattctcag
      301 acccttctgg cggccgataa gttttcgcca ggctgcagaa caggtcgtct ttggcaaatc
      361 cgtcgcattg caccagaacg ccgcgctgaa atacgcccgt gactaggccg gcgacttttt
      421 tcagatgttc caggtcagcg gcactgtcgc aaaagctgtc ttcgcgaagt ttgacggctc
      481 ggccggaaaa cttctgtctg tcgccggcta tcgcgtcaac gtccctgcct tcgacgaaac
      541 tgctgtgcca ctgcttgtag cgtcgcaggg atttgccctt aaattttctg ttttttgtca
      601 cagatgaatt tatacgctgc aatctgcttg acaacaactc tttttccgcc gaaaattccg
      661 gtgatgaatg attcggtaaa atccgttcct gtcataggcc ttgtcggcgg tgtaggcagc
      721 ggcaaaagtg cggcggctga tatttttcgg tcgttaggct gcgaagtcat agatgccgac
      781 gcagtcggcc atgaagttct gcagctgccg caggtggcgg ctgaacttgg taaaattttc
      841 ggccgacaga tttttgatgc ggaaaaccgt atcagccggc cggctttggc gaaaaaagtg
      901 ttcggctgtc ggaatgctga aaatctccac agacttgagg ctgtcgttca tcctgtgatg
      961 tgggagcggt tcatggctgc catccgtgac gcagcgaata aaggcgttat cgccgtcgtg
     1021 ctcgatgcgg cagtgctgtt cgaagccggc tgggacagcc tctgttcatg cacgattttt
     1081 gtggattcgg cggctgacgt ccgccgccgg cgagttttgc agactcgcgg ctgggatgaa
     1141 aataaatggc ggcagcggga aaattcacag ttttcacttg acaaaaaagc cgcgcaatgt
     1201 tgttataaac tctacaacga cagtgacatt ccccacctga aattacaggt aaaaagccta
     1261 ctggatcaaa tcaccggcaa ctaagttttt tgcatcctgt tgtttttccc gtgttgaggg
     1321 caatggcgga gtgttgttat ttcaattctt ccggtttgca tgccttttgt gttttggcga
     1381 taaatgccga tggcttagag cattgaaaag ttgagagttc ctgtttttgc gctagtgggt
     1441 ttgttcgtgg caaggaagga aaatgaggca tatttggtga tattcctaaa aaaattctga
     1501 cgcagccacg aacaaagcaa accagcaaaa a
//
LOCUS       DAONFI010000416         1520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_119692_length_1520_cov_3.767235, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000416 DAONFI010000000
VERSION     DAONFI010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119692_length_1520_cov_3.767235"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLK08_10390"
     CDS             complement(<1..585)
                     /locus_tag="PLK08_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPS53949.1"
                     /translation="MKRKNVKSLAGLRVSVIGLGRFGGGVGVTQWLCGQGAKVTVSDA
                     ASPESLAESIAALDRFDVELHIGGHREDDFTKADLLIVNPAVPKESPLLKLAEKADVP
                     RTSEINLFLERCPAKVVGITGSVGKSTTTAMTGKILATKYRTFVGGNIGKSLLNELDD
                     MNGKDIVVLELSSFQLEDLPIVGISPCVALVTNLA"
     gene            complement(582..>1520)
                     /locus_tag="PLK08_10395"
     CDS             complement(582..>1520)
                     /locus_tag="PLK08_10395"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPS53950.1"
                     /translation="DLLGDILRPSLRQTDFDTEKGVILEEIAMYDDMPHFRVYDNLMA
                     DHFSGHPLGNSILGTKESIKALKRDQMQHYFDRRYSPTNVTLVAVGRIDFDALVKKAE
                     EICGGWKPFDAPRSVTPCDGSRSRKIIVDAKLARENMGFMSPAPSFMSEERFAADVLA
                     GILGDATGSRLYYALIETGLADEASCQYSCMDGSGALMTFISCDPQNAQKVADITSNE
                     YRKFLADGPSEAELAAAKNKFATGATLKGELPMGRLTAVGFDWVYRGEYIPLHDQIQK
                     LLDVTCKDVVALAKECEIDNPTALVLGPTEKMELFE"
BASE COUNT          286 a          448 c          407 g          379 t
ORIGIN      
        1 cgccagattc gtcaccaacg ccacgcaagg acttatgccg actatcggca ggtcttcaag
       61 ctgaaaactc gaaagctcca gaacgactat gtctttcccg ttcatgtcgt cgagttcgtt
      121 tagcagactt ttgccgatgt tgccgccgac gaacgttctg tatttcgtcg caagtatctt
      181 gcccgtcatc gccgtggtgg tgcttttgcc caccgaccct gttatgccga ccacctttgc
      241 cggacagcgt tccaggaaaa gatttatttc actggttctc ggcacgtcag ccttttcggc
      301 gagttttaac agcggcgatt ctttcgggac agccggatta actatgagca ggtcggcttt
      361 ggtgaaatcg tcttcgcgat gtccgcctat gtgcagttcc acgtcgaagc ggtcgagggc
      421 ggctatggac tcagcgagcg attccggcga ggctgcgtcg gataccgtaa ctttcgcacc
      481 ctgcccgcac agccactgcg tcacgcccac accgccgccg aaacggccca gccctatgac
      541 gctgacccgc aaccctgcta gcgatttgac gtttttccgc ttcattcaaa caattccatt
      601 ttttccgtcg gccccagcac aagagccgtc gggttgtcaa tctcgcattc cttcgcaaga
      661 gccacaacgt ccttgcatgt cacgtcgagc agtttctgaa tctggtcgtg cagcggtatg
      721 tattcgccgc gatacaccca gtcgaagccg accgccgtca gcctgcccat cggcaattcg
      781 cccttgagtg tggcgcccgt ggcgaatttg tttttcgccg ccgccaattc cgcctcggac
      841 gggccgtctg cgaggaattt gcgatattcg ttacttgtga tatcagcgac tttttgtgcg
      901 ttttgcgggt cgcacgagat gaacgtcatc agtgcgccgc tgccgtccat acatgaatac
      961 tggcagcttg cttcgtcggc caggccggtt tcaatcaagg cgtagtacag gcggctgccg
     1021 gtggcatcgc ccaaaattcc ggccagtacg tcggcggcga accgttcttc ggacatgaac
     1081 gacggtgccg gactcataaa gcccatgttt tcacgagcca gcttggcatc gacgatgatt
     1141 tttctgcttc gcgagccgtc gcacggcgtt acgctgcggg gtgcgtcaaa cggtttccag
     1201 ccgccgcaga tttcttcagc cttttttacc agcgcatcga agtctattct gcctacggct
     1261 accagcgtga cattggtcgg cgaatagcgg cggtcgaagt agtgctgcat ctggtcgcgt
     1321 ttgagggcct ttatggattc ctttgtgccg agtatgctgt tgcccagcgg atggccgctg
     1381 aaatggtcag ccataaggtt gtcgtaaaca cgaaaatgcg gcatatcatc gtacattgcg
     1441 atttcttcga ggatgacgcc tttttccgtg tcgaagtcag tctgccgcag gctcggccgc
     1501 agaatatcgc ccaacaggtc
//
LOCUS       DAONFI010000417         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_3847
            NODE_121155_length_1509_cov_2.721458, whole genome shotgun
            sequence.
ACCESSION   DAONFI010000417 DAONFI010000000
VERSION     DAONFI010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865818
            Sequence Read Archive: SRR5007244
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 00:50:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,082
            CDSs (total)                      :: 2,051
            Genes (coding)                    :: 2,040
            CDSs (with protein)               :: 2,040
            Genes (RNA)                       :: 31
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121155_length_1509_cov_2.721458"
                     /isolate="AMR_MDS_3847"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="35.92 N 139.63 E"
                     /collection_date="2015-05-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLK08_10400"
     CDS             complement(<1..705)
                     /locus_tag="PLK08_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPS53951.1"
                     /translation="MMKYGIIADFLSSTIGLEPSSLGESGLAHAAQNLMKNTGCENEV
                     ALLEKVKSDTTVLDRLITATTVPETWFFREPQSFAAMEEIATDFYRKNPAGVFRILSM
                     ACCSGEEPYSAAMVLTTAGVPKENYRIDAFDINIFSLEAARRGIYNAKSFRQSSKSYD
                     HFRSRYFTPGNEDAQFILDPEILTQVNFSQGNLTDPCSMPSRYKYNMIFCRNVMIYLN
                     AQARQDVLDYIQNSLFV"
     gene            complement(706..1158)
                     /locus_tag="PLK08_10405"
     CDS             complement(706..1158)
                     /locus_tag="PLK08_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPS53952.1"
                     /translation="MLLMGFNLGSYAYAISCENVVEVIPLIEFRPLPGAPEYVTGIFD
                     YRGSIVPTIDLKMLSMKTPCSQTFGTRIIVIDYPSWDGNKRRIGIAAENVTTTFTAGE
                     NDFENSGVDLKNAPYLGKIVRHDGELVQLISVENLIAPEVQELIFAKR"
     gene            complement(1160..>1509)
                     /locus_tag="PLK08_10410"
     CDS             complement(1160..>1509)
                     /locus_tag="PLK08_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPS53953.1"
                     /translation="TGVMEMDKFSEAMRTGVEQTVRLAGQFSAIITHVEQLTPKFQAV
                     SDGMQSQSQGAQQINDAMIQLTESTRHTLDAVRELNDAADFMRQAVQALGQIIQKFNV
                     VNTAENVREANEE"
BASE COUNT          323 a          370 c          395 g          421 t
ORIGIN      
        1 gacgaaaagt gaattctgta tgtaatccag aacatcctgg cgggcctgtg cgttgagata
       61 tatcatcacg ttgcggcaga atatcatgtt gtatttgtaa cgcgagggca ttgaacatgg
      121 gtctgtcagg ttaccctgtg aaaagttgac ctgcgttaat atttccgggt cgagtatgaa
      181 ctgggcgtcc tcgttgccgg gtgtgaagta gcgactgcgg aaatggtcgt atgattttga
      241 cgactgcctg aacgatttgg cgttatatat gcctcgcctg gccgcttcca gcgagaaaat
      301 gtttatgtca aaagcgtcta ttcgataatt ttctttaggc acgccggctg tcgtcagaac
      361 catagctgcc gaataaggtt cttcgccgct gcaacaggcc atgctcagta tcctgaaaac
      421 tcctgcggga tttttgcgat aaaaatccgt agcaatctcc tccattgccg caaaagactg
      481 cggctcacgg aaaaaccatg tttcaggtac ggtcgtcgcc gtgattagcc ggtccagaac
      541 ggtcgtatcg ctttttactt tttcaaggag agcgacttca ttttcgcagc ctgtgttttt
      601 catgagattc tgcgctgcgt gagccaggcc gctctcgccg agagaggacg gctcaaggcc
      661 tatggtgctg ctcagaaaat ctgcgataat gccgtatttc atcatttaac gctttgcgaa
      721 aataagttcc tgcacctcag gggcgatgag attttcgaca ctgataagct gtacaagttc
      781 accgtcgtgg cggactattt tgcccagata aggtgcgttt ttcaaatcga cgccggaatt
      841 ttcaaaatca ttttcaccag ccgtgaatgt ggtcgtgacg ttttccgccg ctatgccgat
      901 acgtcgttta ttgccgtccc aagacgggta gtcgattact atgattcgcg ttccgaacgt
      961 ctggctgcac ggggttttca tggacagcat tttgaggtcg atagtcggga cgatgctgcc
     1021 gcggtagtcg aaaatgccgg ttacatattc cggcgcgcct ggcagcggtc ggaactctat
     1081 cagcggaatg acctccacta cgttttcaca cgatatggcg tatgcgtatg agcccagatt
     1141 gaatcccata aggagcattt tattcttcgt ttgcctcccg tacgttttcg gcggtattga
     1201 cgacgttgaa tttctggatt atctggccca acgcctgtac tgcctgccgc atgaagtctg
     1261 ctgcgtcgtt aagttccctt accgcgtcaa gagtatgccg cgtgctctcg gtaagctgta
     1321 tcattgcgtc gtttatctgc tgcgcgccct gcgattgaga ctgcatgccg tcggaaacag
     1381 cctggaattt cggcgtcaac tgttcgacat gcgttatgat tgcactgaac tggccggcca
     1441 gacggacggt ctgttctacg ccggtacgca tggcttccga aaatttgtcc atttccatga
     1501 cgccggttg
//
